LOCUS       DAOSCH010000001        68437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_312_length_68437_cov_6.005177, whole genome shotgun sequence.
ACCESSION   DAOSCH010000001 DAOSCH010000000
VERSION     DAOSCH010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 68437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68437
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_312_length_68437_cov_6.005177"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..360
                     /locus_tag="PLZ54_00005"
                     /pseudo
     CDS             211..360
                     /locus_tag="PLZ54_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            739..3357
                     /locus_tag="PLZ54_00010"
     CDS             739..3357
                     /locus_tag="PLZ54_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ65354.1"
                     /translation="MLNVNAAYACQTINEGWQFVKTPGNKTVIPTFENAEKVHLPHTW
                     NMADVWDDVKGYYRDAAWYRKSILVADSLKNKKIFLFFEGANQEAAVYVNGQKAGEHK
                     GGYTAFRVDITNLVDFGKSNRIDVMVDNSYSTEIAPISGDFSMMGGIYRDVYLEVSEK
                     IHFDKMNYAGQGVFVKSFLDGANAGFQIDANIVNSSGETKKLMLRTRITDQKGKIISA
                     QISKSINLKNAMQQNVKQLFAIPDYQLWSPDNPYLYTVECEILDAKTKTVLDTYCVRS
                     GIRTIAKDEKGNILLNGKVIKLIGVNRHQDFDGLSNALPDAIHRRDVEIIKEIGANFL
                     RTSHYPHDPAIYDACDELGILVWSEISVVNQIGINEHFEQNTLNNMKEMILQHFNHPS
                     VVFWGFMNEVLLAANSFPENERGNLFVKTRELAQKLNDVAKSLDNTRLTTIAHHGDFE
                     KYEKAGLNNITDVCGYNLYFGWYVNGFGGFDDFVDNFKQKYPTKPLIISEYGAGSNKG
                     IYARLPRRFDNSMQWAEKLHEIYLDKIIRNPKIAGSSLWNFADFSSEGRKDTDPHMNN
                     KGLLYYDRTPKDVFYLYKAKLQNEPALKIAAGKWLKRPVEVPVEARTFTDTIKIYSNC
                     QQVTLQLNGKTVGNFQPDSICRIFAPLNFVNGKNQLVAIGRSTNGKTITDVVNFDYHF
                     IPFPLNNNSFRELCVNVGGHFDYVDTKSNEVWMPDRPFRPGGWGYVGGEEFKIWKGQR
                     TGGERVIYNTINDPLYQTQRVGLTEYRFDVAAGWYEVELLFAELQSAKELKDAFLNNL
                     GNETKTELLKDGRIFNVSINNQLVLSELNLLESVGENTAANFTFRVKAADNGVVVKFD
                     KIRSETVLNGIKIRKF"
     gene            3360..4901
                     /locus_tag="PLZ54_00015"
     CDS             3360..4901
                     /locus_tag="PLZ54_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOJ65355.1"
                     /translation="MRKFFYVLIACLLVADLSFSKELFYITKFGAKADSKTNNTKAIQ
                     KAIDAAAVKGGIVIVPAGEFLSGTIFLKSNTELHLMQGAKLIASPRIEDYAHLSWGHH
                     EDRTPWHLIVAKDQKNVTISGKGEIYGNGPAYRDPNRKNEFSFFREIEYRPSPLIEIQ
                     GCQQVQIKDILITESPGWTIHLYDTDNAKVRGITIENSLYGPNSDGIDITGSHEVIIS
                     DCNIKSGDDAIAVKTTEDSRSCERIVVTNCVFETNCVAVRIGFESRKDISDITVSNCV
                     IKNTSRAIDVRTVEGGNIRNVFVSGITGKIDSGWAVDRIIEVHSNRIDNPYDIAIKEH
                     PNFGKAKPVEKAGVIENIHFSNMHFRTSGRILVSAISEGLIDNISFENINLTYNLLED
                     AREIGKVAQSVGFARNLPFVRSQNATFVVENAANVRIQNYTVKWPVYPVPDDCLLLTS
                     PNRLYNKWFFDGNEAKIKSGEMRVKYDVFAFRNAKNIQLQNTTSVNSENGAEYFSSEN
                     SIILK"
     gene            5032..6570
                     /locus_tag="PLZ54_00020"
     CDS             5032..6570
                     /locus_tag="PLZ54_00020"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOJ65356.1"
                     /translation="MKRNFALLFILSIFIFSANAIEINVLNEGVVSDSNLVQTAKIQQ
                     LIDKCDKAGGGKIIFPAGAYKTGSLVLKSNITFRVETGATIIASRDTNDYKAVQNNSG
                     DIIPCLLYAKDAQNISIEGNGVIDGKATHYWGDLDMVDGFIKDITENAQKAGVPMKRY
                     YHIKPYTCMIYFENCKFVSIRDITLNASQFWTLHARWTDYMTISGVKVFSDLEKGINA
                     DGIDIDGCRNVTISDCIVETGDDAIVLKTTNLHGESRSCENVTVNNCVLQSSSTALKL
                     GTESHADFRYISFNNCVIRNSNRGLSIVIRDGGTAENIQFSNIMVECSRRHFNWWGNA
                     DPIWLVVLKRRPDSRVGSIRNITFNNIIAHGMGTSKIEGFEDKPLENIRMNNVQFFMH
                     PENYIDKRADDAFRAVNVNDLWLTDVSVKWDTTSVEPLWRNAFTFEKVNVLVLDKLSG
                     EEAPTAKGSFIELRNTDNVLIENCITNKESNVFLKSVSKTNKRVSVGKNIGIGKSKLT
                     ILKK"
     gene            6574..8145
                     /locus_tag="PLZ54_00025"
     CDS             6574..8145
                     /locus_tag="PLZ54_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ65357.1"
                     /translation="MPKLHTISFIVTLFLVSTVFGKKTTSVDICIYGANSGGVIAAYT
                     AQKSGKSVLLIEPSSNLGGLSTDGLGYTDIGNKYVVKGLSRDFYRRLGTHYGKLEQWI
                     FEPSVADGIFNDYVKEADLNVLYNMRLNKVNKSKNTIQSIVIENSIKPSKATNQTISA
                     KIFIDCSYEGDLMARAGASYTIGRESNTLYNETYNGVQLLDAHQFPDGIDPYKEKGNP
                     TSGLLWGISPNPVLPNGTGDKKVQAYNYRICLTDKPSNKVEITKPDNYQPEKYELLIR
                     LKEKQPWNSLNDIFIWSMMPNRKTDINNRGGFSTDMIGMNWNYADASYDERKRIIKEH
                     EDYTKGLLYFVGHDERIPLKIRNEMLKWGYPKDEYIGNSNWSPQLYVREARRLVGEKV
                     MTQHHCQGKEIVSDPIAWAAYTMDSHNCDRHVVNGMVKNEGNVEVGGFPPYPISYQSL
                     VPKRKEASNLIVPVCMSASHIAYGSIRMEPVFMVMSQSAAIEACQAIDAKVGVQDVDA
                     NKVLSEQPMLYYLSL"
     gene            8202..10508
                     /locus_tag="PLZ54_00030"
     CDS             8202..10508
                     /locus_tag="PLZ54_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOJ65358.1"
                     /translation="MNKTFLLICLFLFQLFNYSPLSAKTKFPAYKDPSQPIDNRVKDL
                     LKRMTLEEKIGQMSQISSSELNDSKPGDPKSRRFRPYLDPEKAKKLIKDYHIGSFLVG
                     FAVEPEQWYKFTYELQSIAIQNSRLGIPMIYGNDHIHGANYVIGATIFPQALGLGCTF
                     NKSLAAKMGEVTALEIADLGQPWNFAPVLDLARNPYWPRMYESFGESTHLISEMGTSY
                     IKAMQTAQAALPYKIAATAKHFIGYGESKTGWDRTPAVISDQELYEYHVPPFREAIKA
                     GVKTFMINSSEVNGVPVHASQRLINGLLRKELGFDGVVVTDWADILQLIGQHKVARDE
                     REATKMALLAGIDMSMIATQTTFCDVVKDLVLSKEVPMSVIDKSVSRILKLKFELGLF
                     ENPYPRNDRFKRIGLPENNLAALQAATESMVLLQNDGVLPLKDIKNILAVGPTAHSKR
                     NICGAWTLEWGGAPEEMFPADMETVFTALLKKYPSAVVDTISNPLINLSAFQNKAQIS
                     DIIIVAVGEEPHAEGRGNIEDLTLPVDQLEIIKIVQATGRKHILSIFSGRPRVITSVA
                     EKANAIIWAGLPGYEGATALANILNGDANPSGKLCFTYPRSVGHITPYNVKVHDTYTY
                     KWPFGHGLSYSKYEYGNLILSDSIVKPGQTLKISVQVTNKSEVGGSEAVLWYLSDVVR
                     SISPARTNLVGFDKIYLEPGESKTVNFDITPEKQLTFPNEKGEMLLEDGEFVIKVGDK
                     KQSFYFTKSGHTTPKSGKEGRSHLQEEI"
     gene            10549..12066
                     /locus_tag="PLZ54_00035"
     CDS             10549..12066
                     /locus_tag="PLZ54_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOJ65359.1"
                     /translation="MRKKFVLFATVVTLFSTTPTAYPNIYKPTQLGAIPDGKTLNTAV
                     IQQAIDKANSHGGGTVLLDSGVFVSGTIFLKSNVTLHIAAGATLLGSPRIEDYTELTW
                     GHNVDRQPYHLIVADNAQNITIEGKGTIDGNGEYFWEEYERNADGNMVIPRWIKAKKL
                     KVSPLIEITRSQNIYIHDVTIKTGGGWNLHLFDCDVAKIHGVNIINNLYSPNSDGIDI
                     TGCNDIIISDCYIKTCDDAICLKTTPDSRSCHRVTVTNCVIETLCVALKLGCTESSKD
                     ITDVTFNNCVVNKSSRAVMIGVRDGATYDRIAIINIVANTNAPLVFNRPIQILVNKLN
                     PDSKAGTVRNVLISNFICETEGRILLTSAEGGLIENVILRDIVLRYPMIESPQRYIGA
                     KVSSQFPKPTDHPEAVTANAALVAENIKNLVIENMIVDWPLTDSTPINWQHPERIENG
                     TSHIHKPDYTKARQTEFSAIWARNVQGGYFRTPLLKSSSKTKPVFDIKKSNIKIL"
     gene            12109..14292
                     /locus_tag="PLZ54_00040"
     CDS             12109..14292
                     /locus_tag="PLZ54_00040"
                     /inference="COORDINATES: protein motif:HMM:NF020121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase trimerization
                     domain-containing protein"
                     /protein_id="HOJ65360.1"
                     /translation="MKKLLFILILIPILLPAQKNDEFLHALYDKLHDSPMQQKIREIA
                     PMPFGVVFLPWAGCTEQDMRQHFRMMKRLGFHNLKQTMATPEWSEKEILRIALEEDVI
                     PFWYGEGGWEPITTDLLRKLNIPLSATKEEIRKNPKMLEYQKNVFQKQLADWKKPISD
                     TTLIPYRHEPDAYLRPSDINLFKQWVKNKYQSIDQLVTAWNQYEVGIKKIPYRSWNDF
                     ENDKFLFSAPSNDEIIPSSGREYGFVKDVLRFKADMYLKNVKNFVVSNHPEQPVRAGG
                     EMGLFLPFASRAIDMEGIADVMKDRGSFYPSIHLAWHFEEVDYEVTRCVYMQSSLATD
                     WFKGGWAAVWESTGGPQQFSGGKGWDRKGAESTAGFTVDGGTITQMLLSYLAGGLKGV
                     GLWAWNYRRAGWESGEYALLNRQNEPGERAIRAGQIAQAAEKYRDELWAAHKEPYVGV
                     FVNWDNEAIWAACAVPNRTHFKNYPVQARIGISRALINANIPWEHVTPSDLKAGLAMR
                     YKVIYLPAQVAINEDLFPIFEQYVRDGGRLVLDAPGAWWNEQGKVLDTGKGSAFERIF
                     GASIADYQYANNVKPQLANLNLTGFLLDIKPTSADVLQKFNNNAAAVTEHRLDKGSAI
                     ILSPDASFSMKNKGNEPMENWVINVIMQDIRKPYECKNAIVYRLGSEMADHYFILNDG
                     SAKNVSLKLNRYKYRHAIDAVTQQKVSISSINIEANGGRWIRCEK"
     gene            14341..15852
                     /locus_tag="PLZ54_00045"
     CDS             14341..15852
                     /locus_tag="PLZ54_00045"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOJ65361.1"
                     /translation="MSKFSWFIVVFLFLSSCKSLSGYYDVTDFGAKNDSTKLSTKAIQ
                     KAIDKANANGGGKVVFPQGVYLTGTIVLKDNVTLYFNEGSRLKGSDKHADYLFHKTKI
                     SVYPNPNRAYLIYADGVKNISIEGKGVIDGNGTKFWEPFDTLPRWIKPLNGRVSNMIE
                     INNSKNIVIKGVTLSNSPEWTCHLFDCDDVLVEGIRLVNNLYGPNNDGIDISGCNNVM
                     IANSYIETCDDAVCIKTFPSSRESSNITVTNCIMKTTCVALKLGENFKRIADVTFSNC
                     IIYESSRAIGIYGDCGGTTENINISNIVCNTNAPLILNRPIQIGIWKGKGNPGKIRNI
                     SISNFTATTEGRIMLTAIEGSLIENITLRDVNLVYPMIEDPSKYAEGTTSNQFSSIDI
                     DAKKAKAAIVAQNVNGLVIHNLNITWPHDTIPTQWKFVERIENGSNRVHRPDYATPKA
                     TEFSIVWAKNVDSGYIYAPLAKPSAHNVKDYLLINSNIKIKQTDTIRYDEKPL"
     gene            15870..17204
                     /locus_tag="PLZ54_00050"
     CDS             15870..17204
                     /locus_tag="PLZ54_00050"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HOJ65362.1"
                     /translation="MRFLFITLLFSNFVLSSICQQNHTLHPDKRWFKDAKYGVFVHWT
                     LNVSHKDRERMPYEEFQIKCFDEAKKFTANNYDPKLWAKMFKSWGAKYVVLTTKHHLG
                     FALYDSEYNQFTALKSTPANRDLVREFCDAMRYEGIKVGLYFSLPDKFNYDYPTLVTQ
                     NNGKEVIWQQSDFVAWKRFTDNMLNEIKHLCTNYGRIDLFWFDGDWERNADMWRSKEI
                     VDTINKYQPWAVLNNRLRSANIGDFSTPEMVIPIDKRNGMWELCTTLGYNWDGPESQK
                     SIKKPSETVRILGDVITQGGNLLLNISPDYSGSISQPQIKSMELLGKWITEHAEAIYD
                     TEQGLPLGLFNGGSTRRGSTIYLIAYETAPELVLKNIEGEIESIVHLKSGKHLTWRNM
                     DDYHSDNNRKGWRFISLPTNLIEPYATVLKITFKDKKVKVKKHDGTYLEWKE"
     gene            complement(17731..19374)
                     /locus_tag="PLZ54_00055"
     CDS             complement(17731..19374)
                     /locus_tag="PLZ54_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65363.1"
                     /translation="MKKLVLLAFIFSTIFAFGQLPTFKISKKVIADIDDKLFGQFMEK
                     PSWDGEIGGDGAIDKKTGSVSPDVLAYLKEMKIPIIRYPGGTDVDYYQWYNLIDNVPG
                     KHQKRPAYRNYSGTGKITSDNRLGLDAFLTLCSELKSEPLLVVNIGDAFFRKISIEES
                     AKNAAGFVAYCNSKVGTKVSEGMPDWPAIRAKNGHTKPYNVRYFEIGNEIQLFKSMKE
                     KDVNDSLVNHYVNCLEAIVNAMLDVDPNIKIITDGEIKEVSEQIKRRLGNKISYLACH
                     YYLPWEINTVKLDTTTIDIKSLTQADIWNAWVATPYIHPETGQSYFPKTGKYNHAADA
                     GYDLAITEWNWNGWWNKKILPDAALNSLFAKGIGAAGFIHAMMRDAGRMKIGCQSMLV
                     GNVWDINAIRVDTTGKMKARFFPTGKVTTLYSKYHGNQLLEMSEKNQLFYTQPYKMNS
                     IEAVPRVTYVDALASRNETTLYISFINRNFANDMDVEIDLSDFKTDSKYKQHIFTTND
                     MITNELGYFEDKTEKVKNHKPLIVHLPKHSLSIFEIKIF"
     gene            complement(19522..20823)
                     /locus_tag="PLZ54_00060"
     CDS             complement(19522..20823)
                     /locus_tag="PLZ54_00060"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOJ65364.1"
                     /translation="MLTAQNPICPPGLNIADPSARVWNDGRLYVYGSRDESFNYYCSF
                     DYWVLSTDDLIHWQYTPNVFASKGQYDQVPFNDNLLFAPDCMYKNGTYYLYYCQPDGI
                     QGVATSLSPIGPFTNAKEIYLKNKNQIDPAVFVDDDGQAYYMWGQFQAKIAKLKPNMT
                     EIDTTTIVDNLVTEKEHFFHEGGYMVKRNGIYYFVYAHVGRKNMPTCIGYSTSKSPLG
                     PFKYGGVIIDNDGCDPANWNNHGSIVEFKGQWYVFYHRTTNGCVNSRKACIEPIYFNT
                     DGSITEVEMTSQGAGKPLDAFSKIQAEHACLLSGHVRVESYSFDTNNPLNPTNSDQLG
                     QIEDKDRAMYKYIDFGEGVDSVSIRIAPGLNSGKISFILDNSWSLPFAEIAVDGGGDG
                     KTWKIFSCKVKRVNGIHALNLTFSSTGSNTFNIDWFTFKHQ"
     gene            complement(21326..22612)
                     /locus_tag="PLZ54_00065"
     CDS             complement(21326..22612)
                     /locus_tag="PLZ54_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 140 protein"
                     /protein_id="HOJ65365.1"
                     /translation="MSYKNGDPFFWLGDTAWELFHRLKLEEIETYLENRKQKGFNVVQ
                     VVVLPEMDGLRVPNRYGDVPLIDMDPTKPNEKYFQFVDTVVKLAAKKGIFMGLLPTWG
                     DKVTQLWGEGPVVFNPENAYKYGKWIGERYKSYKNIIWILGGDRPPMKDSADWRPVWR
                     NMAKGIEKATNHSALISYHIWGGEMSTSQYIHSEKWLDINMMQSGHGSGKDVPVWEWI
                     KRDYCMKPTKPTLDSEPNYEDHPVNPWPSWNPENGYFRDYDVRKQTYRSVFAGACGVT
                     YGNHSIWQFRNERDPNINFAERYWTEALDRPGAFQVGYLKKLIESRPSSSRIPDQSII
                     LEGQGEKEEYICAFRNRSNDYAMIYIPVGKTITIKTSFIKTKKLKISWFNPQTCETIQ
                     SIETINTGKMCVTTPTTGFGKDWVLIVEKDLVNTAK"
     gene            complement(22717..24153)
                     /locus_tag="PLZ54_00070"
     CDS             complement(22717..24153)
                     /locus_tag="PLZ54_00070"
                     /inference="COORDINATES: protein motif:HMM:NF013377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HOJ65366.1"
                     /translation="MKKVSRYYFIFSALALLLLFSGCRSSQKNSKTSKTGFTPEAYTA
                     LYADKWSAINDSVKAGWKNHSGKPSNNAVPDTFAYAFNEWTLFYWDLYFTQIGLLKHN
                     ELKLAKGGINNLKAELDKRGFIPNANTDWGDNRSQPPYFGIMVKEFYDFTKDKKWLKT
                     MYAAVLKEYKFWTDTSANRIEANTTELGLQRYFHHATETELLELYDQELTSRFDFPKD
                     IEKSKKLNIASQFAAEAESGMDFTPRFENRCSEFIAVDLNSNLYLYEKYLAYFEQELK
                     IGNGQQWLAKARKRKALIQKYCWNDERGLFLDYDFVNNRSSKVAAATAFSPLFAGLAS
                     KYQADRMRENLHLFESENGIVACESTDEKRIYQWDHISVWSPMQSLVIMALDNYGFKE
                     DACRIAMKHLDLITANFFNPNPSTYVDKSGKKFYRLPGGIYEKYTRFGTINDREYNAS
                     LMYGWSAGAFAFAYDYLIVNSQHNFTIK"
     gene            complement(24166..25884)
                     /locus_tag="PLZ54_00075"
     CDS             complement(24166..25884)
                     /locus_tag="PLZ54_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ65367.1"
                     /translation="MKFSFLSSLSLIIVSANICLSGFAQCGKGEYTISAFPCLNCAVT
                     CQKPAGPEWIHNATFYQIYPQSFKDTNGDGIGDLKGVISQLDYVKSLGVDAIWINPFY
                     VSPFRDAGYDVADYYHVDPRYGTDQDAKDLFRIAKQKGLRIIVDFVPGHTSIDHPWFK
                     ASCSSTPTKYSNWYIWTNGTWFDDENGKYKANFIQGFCDRDGNYMINFFWHQPALNYG
                     YGQPDPKQPWQLSTTHPDVVALRTEMKNVMRYWLEMGCDGFRVDMAGSLVKNDPEGSI
                     KYFWQDIRKMLDQEYPDAFMVSEWSESATAVNAGFHADFMLWQEGYQDLFMHENSYFR
                     SAGIGSISHFMWRYNEQSTETNGKGYISLTVGNHDLERINNRGRNRADLEIIQVFNFT
                     MPNIPFIYYGDEITMRQLPLVTGKEGCYGTRAGARTPMQWDTTANLGFSTSAPERLYL
                     PVDSSRNAPNVLAQENDPDSFLNFMRKLVLLHKTEPALTNYAKFVPVYVQKNKYPFIF
                     MRIFGNEKLLVILNPDSKPATAIVEGNLKGKRKLLLGKLPVWKQNTGTTEIQVDGQSY
                     SVIKLK"
     gene            26153..26320
                     /locus_tag="PLZ54_00080"
     CDS             26153..26320
                     /locus_tag="PLZ54_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65368.1"
                     /translation="MVEWITDDATPYNNLTKNVKSHHAQVIKKDDMPKILPWLYIAIG
                     NVKRLLLDVHH"
     gene            26551..29283
                     /locus_tag="PLZ54_00085"
     CDS             26551..29283
                     /locus_tag="PLZ54_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ65369.1"
                     /translation="MKKILFTFSLLLCYLLSWGQIITTNPTFITKDYPGEIEIIYDAT
                     QGTAGLKDYTGDVYAHTGVITSSSTSPSDWKHAPVWGDNSPKYKLTSLGNNKWKLLIT
                     PNMAGYYNLSPGEVVRKLAFVFRNSTGTLQGKDIGGADIFVTVYESNFNVAFTFPTSN
                     QSVAIGSSIDFTVSSSMSAELKLLLNNTVLATQQAATSLTYSYNFSVAGDYTFIASAK
                     VDNNTVYDTLLVCVPEPVVNQTRPAGTKLGINYIDNTTVTLVFHAPNKTNVFLIGEFN
                     DWAQLNAYQLKKDGEYWWITLTGLTPGKIYAYQYLVDGTIRCSDPYTELVLDPWNDKW
                     INQYYTIYPNLKPYPEGKTEGLVATFQTAKPAYNWEITNFSMPAKENLVIYEMLLRDF
                     TTEKSLQAAIGKLDYLKNLGITAVELMPIQEFDGNNSWGYNPNHYFAPDKAYGTPEMY
                     KKFIDECHKRGMAVILDMVFNQASGICPFALLYWDSTKNRPAADNPWMNPVAPHQFSV
                     LNDFNHSYSGTKEYFKRVLQYWITEYKVDGYRMDLTKGFTQNSGTESTWDQTRINNLK
                     DYYYAVTDVKPDALFILEHFVGGSEEDYLAAQGMYLWRNLNNAYSQAAMGFQSNSDFS
                     GMNTSPRRWVGYAESHDEERNFYKAKTYGNGTVKTDSLYRISRVPLVVAFATLMPGPK
                     MLWQFEEMGYDYSIDSFGGRTNPKPHAWGWLNLPHRKAAADACAKIISMRKLYPNAFL
                     NGNYSLQVAYNDWSQGRRIALSHADLNMVVLGNFQSDASITANPSFQRTGTWYNLLTG
                     ETLNVSNTNMTINMQPGQLLIFTDRNVNFTNDNREISYNVSDYVYPTLTSGKLFISST
                     ANIQSIRIYNLQGSLLKQQNNDIKEIDLSMLKKGFYLVELETANGKSAHKIIKE"
     gene            complement(29624..31342)
                     /locus_tag="PLZ54_00090"
     CDS             complement(29624..31342)
                     /locus_tag="PLZ54_00090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOJ65370.1"
                     /translation="MKNYVVSARKYRPTTFKSVVGQTALTNTLKNAIKSNHLAHAYLF
                     CGTRGVGKTTCARIFAKTINCEHLTEDFEACNECESCKAFNEQRSYNIHELDAASNNS
                     VEGIRSLIDQVRIPPQIGKYSVYIIDEVHMLSSSAFNAFLKTLEEPPAHAIFILATTE
                     KHKIIPTILSRCQIYDFNRISVSDIVGHLQYVAHEEGITVDVNGLNVIAQKCDGGMRD
                     ALSIFDQLVSLCGNNISYQDVLTNLNVLDYDYYFQLIEAFMKNDVNTSLLIFNDIIQH
                     GFDAHHFVSGLSSHFRDLLVSKDPQTIQLLEVGADIGKHYQMQAQQCPEDFIFKALKI
                     CNDCDLNYNISKNKRLLVEIMLIRLCQLVKQEDKLNGEEKNEPLKKVVDELKTNNVVL
                     AKNSPSSTKVQDSTSNPMSTDVDAKQVKTERPTSISIKNNVVSSPSSSYEAPQKEQPD
                     IRNEIFTQDDLQKAWAELAKTLVDEARMTAFMQSNRPELISNTEFRILVTNELQEKEL
                     KRLKPTILNFLKTALKNSNIKMSIQIQEDRSVQKGRTPEEKYKIMAEENPALAKLRND
                     LQLEID"
     gene            complement(31915..32913)
                     /locus_tag="PLZ54_00095"
     CDS             complement(31915..32913)
                     /locus_tag="PLZ54_00095"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOJ65371.1"
                     /translation="MSKINAVITGVGGYVPDYVLTNEELSTMMDTNDEWITTRVGIKE
                     RRILKDKELGTSFMASKAVEDLLSKSGTNPDEIDMVLCATTTPDYIFPACAAIVTQNV
                     GIKNAVAFDIQAACSGFIVGLTTATGLIESGRFKKILVIGAEKMSSITNYEDRTTAPL
                     FGDAAGAVLVEPTTENVGIIDSILFTDGVGISHLNLKGGGSVYPASHETVDKRMHYIY
                     QEGQFVFKHAVSDMSDATVKILERNHLTKDDIAWLIPHQANLRIIDAVAQRIGLEYEK
                     VIINISRYGNTSAATIPLCMWESEKKFKKGDNIILTAFGAGFTWGAVYLKWGYTKE"
     gene            complement(33024..33209)
                     /gene="rpmF"
                     /locus_tag="PLZ54_00100"
     CDS             complement(33024..33209)
                     /gene="rpmF"
                     /locus_tag="PLZ54_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOJ65372.1"
                     /translation="MAHPKRKHSNQRTAKRRTHDKAKLPTLAICTNCGATYIYHTVCG
                     ECGYYRGKLAIEKLANA"
     gene            complement(33229..33798)
                     /locus_tag="PLZ54_00105"
     CDS             complement(33229..33798)
                     /locus_tag="PLZ54_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOJ65373.1"
                     /translation="MSKFELYNIVLKDLTNEKRIVEFDLDDAYFQKIDSPEVQQGNVH
                     AKITVQKKTTVYKLDFELNGFITLPCSRCLEDMQQLIHYKESLEVKFGNKFSEENDIV
                     IVPEADGAINVAWFLYEFIVLNIPLKHVHAPGECNKAMITKLKKHISSTKEYEDETSL
                     ELEDTNEDDYFSNNQFIDLEFDNLQNIDN"
     gene            complement(33922..35076)
                     /locus_tag="PLZ54_00110"
     CDS             complement(33922..35076)
                     /locus_tag="PLZ54_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ65374.1"
                     /translation="MFSFNKIYRLSQRLRLVFIGSAALIVTASLFFTNRLTRQLATEE
                     HKKMEIWAGAMRQVILAGENTDINFLSEIISGNTTIPVIMTDENDNLISSLNVNEPAS
                     KTNDYYRREIERLKKKNSHIEVRIDNTLQHIYYDDSLLLKQLRYFPYIQLGVIFIFLL
                     IAYLAFSGTKKAEQNQVWVGLSKETAHQLGTPISSLLAWLDLLRIRNDDKLLTEMEKD
                     VNRLKIIAERFSKIGSAPDLQLVKLNETITNAVQYISHRSSQKVIITCHFQTSDNDLI
                     RMNVPLFEWVIENLCKNAIDAMNGNGKIDIFVQKKSNEYIVDVKDTGKGIEKNKFKAV
                     FSTGYTTKPRGWGLGLSLAKRIIEEYHKGKIFVKQSEIDKGTTFRILMKA"
     gene            35484..36908
                     /locus_tag="PLZ54_00115"
     CDS             35484..36908
                     /locus_tag="PLZ54_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric [FeFe] hydrogenase"
                     /protein_id="HOJ65375.1"
                     /translation="MAFLNNTLIVRRDLMTKLVSLFNEGVLLSEIDRVPLKMSPKKAQ
                     ARGRCCIHKERAVLKYKIMPILGYSIEEEEDELTPLSEYARKALLRTEKNKSIFTVVD
                     EACTACVKTNYVVTNLCKGCVARACYMNCPREAVFFKENGQAGIDHNKCINCGICKEA
                     CPYHSIVFVPVPCEESCPVKAIQKDENGIEHIDESKCIFCGKCINACPFGSIFEISQI
                     FDVLSAIQRNEKITAIVAPSVQSQFEHSFSDIATAIKNIGFSEIIEVALGAMETAFHE
                     GEELQQKLEQGQQFMTTSCCPSYVELTKKHLIEMKPFVSQTNSPMYYASKIAKNKYPD
                     SKVCFIGPCVGKRKEAADDQTIDYVLTFEELDAILKGMKIEIIPTENKDDEEIKAGRG
                     FAQSGGVIAAVMESYPNVGIKPLIVTNIDKKNIALLKAYAKGKAPGNFIEVMACEGGC
                     ISGPCTINTYKDAQKIFKKVVEEN"
     gene            37035..38120
                     /gene="prfA"
                     /locus_tag="PLZ54_00120"
     CDS             37035..38120
                     /gene="prfA"
                     /locus_tag="PLZ54_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOJ65376.1"
                     /translation="MAEKTLLEKLEGLQNKFDEISTLITDPNVISDQKRFAKLNKEYH
                     DLREILNVKNEYELALSHLTEAKQMVETESDTEMRELARSEIEELEQKIPELENKLKF
                     LLIPADPEDRKNAIVEIRGGTGGDEAAIFAGDLFHMYAKFCESKGWKIEVTDYNEGTA
                     GGYKEIIFTVSGENVYGTLKYESGVHRVQRVPQTETQGRIHTSAATVAVFPEAEEFDV
                     DLKESDIRVDIYCSSGPGGQSVNTTYSAVRLIHIPTGITVQCQDEKSQHKNKAKAMIE
                     LRSRIYAIEHQKYLDEIGSRRKTMVSTGDRSAKIRTYNYPQGRVTDHRINYTIYNLPA
                     FMNGEIQTMIDQLMIAENAERLKDAEL"
     gene            38150..38986
                     /gene="pyrF"
                     /locus_tag="PLZ54_00125"
     CDS             38150..38986
                     /gene="pyrF"
                     /locus_tag="PLZ54_00125"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOJ65377.1"
                     /translation="MNRKELFENIKRKKSFLCIGLDTDINKIPIYLKEKSSNPIFDFN
                     KVIVDATADLCVAYKPNLAFYESLGIEGLKALNETVAYIRNHHPDQFLIADAKRGDIG
                     NTSAMYAQAFFEKMNFDAITVSPYMGEDSIKPFLSYNSKWTIILALTSNKGAFDFQLI
                     KEENTRERLFEKVLKTSTNWGTEENIMYVVGATKTEYLKSVREIVPEHFLLIPGIGAQ
                     GGSLEETAKFGLTSQCGLLVNSSRQIIYASSDENFAEAARFEALQLQQAMENILLKSG
                     II"
     gene            complement(39066..40286)
                     /locus_tag="PLZ54_00130"
     CDS             complement(39066..40286)
                     /locus_tag="PLZ54_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ65378.1"
                     /translation="MKKILLSVMLLAVAVTYASAQSTAETKAKADIAHWSAGIKAGVD
                     YYRVTPSSTVTDYLFFENSWARYANNAAWTAPQIYVEYTFNPLFGLGADFSYLTYGRN
                     TGAGHTLDFTLYGSTNLSNLLAPKRAGFWGKVNIYGNLGAGLGFYSYSGVAGVTDGDN
                     LKSPLAFAALNAEYRLSKVLSLGVEGQYRYYMRENLGGLSNNSLGSDALIGTIGLRYK
                     FGGATKSHVRDMSLEEYYPAPAPVVIEKTVQDGKVDANTENRLKALENDNAMIKNKLQ
                     QLESDLKALSQKSTGTVNATFQNIEFEFGSSKLTKQAYDLLDQIYAILNSNSTAWTKI
                     IVSGHTDNIGPKSVNQQLSIARAQAVKDYLVKKGIPASAITVAGYGEDKPIASNDTPA
                     GRQKNRRVEFEISK"
     gene            complement(40676..41305)
                     /gene="pdxH"
                     /locus_tag="PLZ54_00135"
     CDS             complement(40676..41305)
                     /gene="pdxH"
                     /locus_tag="PLZ54_00135"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOJ65379.1"
                     /translation="MLKDLRKQYDRSTLNESNILKNPFQQFKLWLSEAINTEESEPNA
                     MVLSTVDAEWQPHGRMVLLKELTDEGFVFFTNYKGNKGLQILHNQRVSLLFFWPVLER
                     QVRITGNAEKIAEQASDEYFKMRPLFSQIGAWASPQSRKIPSRDFLDKQFEYYKHKFE
                     TEVPRPPFWGVFLVRPKTFEFWQGRPDRMHDRLFYTKNDQQWEISRLAP"
     gene            complement(41411..42292)
                     /gene="era"
                     /locus_tag="PLZ54_00140"
     CDS             complement(41411..42292)
                     /gene="era"
                     /locus_tag="PLZ54_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009228927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOJ65380.1"
                     /translation="MHKSGFINLVGNPNVGKSTLMNALVGEKLSIITSKSQTTRHRIL
                     GIVNEENYQMIFSDTPGILKPKYALQESMLQFSRSALTDADILMYVTDVYDAEPKDMD
                     FVQKINSDNVPLLVLINKIDLINQEKLELLVEKWHGIFPRGEILPISALEKFNVDYLM
                     KRIKELLPESPPFFDKDQLTDKPVRFFISEIIREKVLLNYQKEVPYAVEVEIEEFHEE
                     ENLVRIGAVIYVERDSQKGIIIGKGGKSLKKVGVQARKDIEEFLGQKVFLSLYVKVEK
                     DWRNKEKKLKDFGYLHD"
     gene            42646..43101
                     /gene="rplM"
                     /locus_tag="PLZ54_00145"
     CDS             42646..43101
                     /gene="rplM"
                     /locus_tag="PLZ54_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOJ65381.1"
                     /translation="MDTLSYKTISANKNTVQKEWVLIDASDEILGRMGTKVAKLLRGK
                     YKPNFTPHVDCGDNVIIINASKIKLTGNKWTDRIYLRHTGYPGGQRETTPAQMLAKDP
                     ERLIRKVIKGMLPKNRLGAKLLKNLYVFPNAEHNMQAQQPKQIDLNSLK"
     gene            43107..43493
                     /gene="rpsI"
                     /locus_tag="PLZ54_00150"
     CDS             43107..43493
                     /gene="rpsI"
                     /locus_tag="PLZ54_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOJ65382.1"
                     /translation="MEIVNALGRRKAAVARVFVNEGTGKIIINKRELSVYFPSLLLQY
                     IVKQPLDKLNVTEKYDIKVNLKGGGFKGQAEALRLAIARALVKINPEDKPALKSEGFL
                     TRDPRIVERKKPGRPKARKRFQFSKR"
     gene            43662..44498
                     /gene="rpsB"
                     /locus_tag="PLZ54_00155"
     CDS             43662..44498
                     /gene="rpsB"
                     /locus_tag="PLZ54_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOJ65383.1"
                     /translation="MSRTNFEELLEAGCHFGHLKRKWNPAMAPYIFMERNGIHIIDLH
                     KTAAKIDEAAQAMKQIAKSGRKILFVATKKQAKDLVAEKAKAVGMPYITERWPGGMLT
                     NFPTIRKAIKKMSTIDKMMTDGTFDNISKREKLQITREREKLEKNLGSITDLTRLPAA
                     IFVVDVMKEHIAVKEAEKLGIPIFGIVDTNSDPSNIDFVIPANDDATKSIEVILNALI
                     DAIQEGIEERKLEKVETEAAEAPVVKERKSRVNKRPRTQREDDEALKANVVNKFIKEE
                     EN"
     gene            44718..45536
                     /gene="tsf"
                     /locus_tag="PLZ54_00160"
     CDS             44718..45536
                     /gene="tsf"
                     /locus_tag="PLZ54_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOJ65384.1"
                     /translation="MAVTLQEITKLRTMTGAGLMDCKNALIEANNDLEKAIEIIRKKG
                     QAIAAKRSGREASEGCVLAAAKDGFAAVLALKCETDFVAKNADFMALTQSILDVAMDK
                     KPATLDELKAFTINGRTIADLVVDESAITGEKVELDDYNFVKGGTTAAYIHQGNKLAT
                     IVAFKEENVDHQVARDIAMQIAAMNPIAIDRASVPAHIIETELEIGREKAREEGKPEN
                     IIDKIAEGRLNKFFQESVLLEQTSVKDSKITIGEYLKAHHATIIDFKRVSLNLD"
     gene            complement(45639..46982)
                     /locus_tag="PLZ54_00165"
     CDS             complement(45639..46982)
                     /locus_tag="PLZ54_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOJ65385.1"
                     /translation="MSKIYDFTLSYSLFKSYVMFAFRKFFSRYIVMGKENIPETGPVI
                     FAPNHLNALMDALAVISVVPHKMPVVFLARSDYFNNKLIARFLHFCKILPAFRMREGL
                     ENVDKNNEVFEVCVDVLNHNQAIGIMPEGNQGSKRKLRPLAKGIFRIAFMAQQKYGNQ
                     PGVKIVPVGLDWGNIDKYGEHIIINVGKPIEVCEYMNEYEENQPAAINQLRDRLRDGL
                     SEVTLDIATEKNYECFETATIAASAAMVEKMKLPNTEKYRFLARQKTARQLLEMEKNK
                     PETIDKLNVICKEYRENLRKLNVPSWLLDKNLKVSSLLLESLLLVITFPFFLIGLVLN
                     ALPFFSPDIIMKAFHINFPGGKGSLRFGLGMVTFPLFYILQSVLFYKKVKDSLWAVAV
                     FLPLQYYLGKCAFLWHKKFRKLKVKFRYHKLQVKNSLILQRTKLLHEEILQLVQS"
     gene            complement(47250..49088)
                     /locus_tag="PLZ54_00170"
     CDS             complement(47250..49088)
                     /locus_tag="PLZ54_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ65386.1"
                     /translation="MFKFFELLKRFIPPYKKYVFLNLLFNLLSTVFSLFSFAAIIPVL
                     QILFGLQQSNLSYQAWTWGDGWNQIISALKNNIFYYLEQMMTTAGPSMTLLYLGLFLI
                     VMTVFKTGTSYLSSYYIIPIRTGVLRDLRNQLYKKILSLPIGFFTEERKGDIMSRMTS
                     DVTEIETSIISSLEMIFKNPIIILIYLTVMFAMSWKLTFFVLILLPLSGWLIGMIGKS
                     LKQRSTLGQQQTGELLSQIEETLSGLRVIKAFNAEKKLEKRFAALNEKLRRTLNRINR
                     RYTLAHPLSELLGTVVIAILLWFGGVLILENHSSINAAEFIYYLVIFYSIINPAKDLS
                     KAGYSVQKGLASLERVDKILKAENTLPEPTESKKINSFNDRIEFKNVNFRYQSDWVLQ
                     DINLTIPKGKTVALVGQSGAGKSTLVDLLPRFYDVTEGEILIDGINIKDLNTKDIRSL
                     MGNVNQEAILFNDTFFNNIAFGVENVTLEQVMEAAKIANAHDFIMASENGYQTTIGDR
                     GSKLSGGQRQRISIARAILKNPPILILDEATSALDTESEKMVQEAIEKLMMNRTTLVI
                     AHRLSTIKRADEICVMSEGKIVERGKHDELIALNGYYKRLYDMQSF"
     gene            complement(49126..50469)
                     /gene="purB"
                     /locus_tag="PLZ54_00175"
     CDS             complement(49126..50469)
                     /gene="purB"
                     /locus_tag="PLZ54_00175"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOJ65387.1"
                     /translation="MNLSPLTAISPVDGRYRSKTEKYAEYFSEFALIRFRVMVEVEYF
                     ISLCEIPLKELQSVPKDCFDKLRAVYIHFQESDAEKIKEIEQTTNHDVKAVEYFLKQQ
                     FDLLGLSDYKEFIHFGLTSQDINNTAVPVSIKKALEDVYYPEMENLLKKMRELADEWK
                     DIPMLARTHGQPASPTRLGKEIMVFVYRLDKQLSLLRAVPISGKFGGATGNFNAHSVA
                     YPSIEWKNFADKFLAEKLGIQREEWTTQISNYDHLAALLDSLKRIDTILIDFCRDVWT
                     YVSMEYFKQKIKPGEVGSSAMPHKVNPIDFENAEGNLGMANAVLDFLSAKLPISRLQR
                     DLTDSTVIRNIGLPFSYILIATQSLMKGMNKLVLNESAIRRDLENNWAVVAEAIQTIL
                     RREGYPAPYETLKALTRTNEVITEQTIHDFIDSLQVSEEVKNELRKISPFSYTGI"
     gene            50761..51432
                     /locus_tag="PLZ54_00180"
     CDS             50761..51432
                     /locus_tag="PLZ54_00180"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOJ65388.1"
                     /translation="MKKSIRIHKEGWFILTGLLLLLFAVNLIIYMEFPGPAMAINTVL
                     SAIILCFFVFFFRNPKRIVEIDDPSFVIAPADGTVVVVEKTLEPEYFGDERIQVSIFM
                     SIFNVHANWYPVSGTVVKTEHHNGRHLAAYLPKSSRENERSTVVIETPGKTQILVRQI
                     AGALAQRIVTYAHPGKECHVNEHLGFIKFGSRVDLFLPLDSEIFVQVGEKTTGNETII
                     ARLNE"
     gene            51444..51770
                     /locus_tag="PLZ54_00185"
     CDS             51444..51770
                     /locus_tag="PLZ54_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOJ65389.1"
                     /translation="MTLQEAQQIVDNWIKTYGVRYFGELTNMAILTEEVGELARVMAR
                     TYGEQSYKAEDEKYDLADEMADVLWVLLCLANQTGVDLTDAFSKNIEKKTLRDAQRHI
                     QNKKLY"
     gene            51794..52681
                     /gene="deoC"
                     /locus_tag="PLZ54_00190"
     CDS             51794..52681
                     /gene="deoC"
                     /locus_tag="PLZ54_00190"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOJ65390.1"
                     /translation="MKDRYEELLELYQCESVDDEAIKIDIDNILAEHFAENASKDVYA
                     KCLNLIDLTSLNSTDTEGEIVSMVEKVNHFRGTFPHLSNVAAICVYPALIPAVKSHLT
                     EENVGIASVAAGFPSSQTFIEVKIAETSMVVNEGASEVDVVISVGKFLEGNYGEVYEE
                     LSEIKAACRNAHLKVILETGALKNSVNIKKAAILAMSAGADFVKTSTGKIQVSATLEA
                     TYIISHAIKEWYEKTGQKIGYKPAGGISTTEEAVKHYTLVKHILGEEWLNNRLFRFGA
                     SRLANHLLSSIEGKEIKYF"
     gene            complement(52686..53660)
                     /locus_tag="PLZ54_00195"
     CDS             complement(52686..53660)
                     /locus_tag="PLZ54_00195"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ65391.1"
                     /translation="MNLIEKIRKPIEKEIEQFEKSMTEELQTENPLLKNISNYLQLGK
                     GKQLRPILVILSAKLCGEVTESTIYGAISLELIHTASLIHDDVVDNTFQRRGQPSINA
                     QWNNKVAILVGDHILSKSLSSGIKADNIKILETIANVGMNLTDGELLQLDKVQHAQIS
                     EEDYLEIIQKKTALLFSSCALIGGLSVKADKNTLKHLYHFGEYAGICFQMKDDLLDYS
                     ENNEIGKPTRNDIKDGKITLPLIYALKNATNHQKHEIEQWIENSDFSDLHIEKIVQFV
                     QENGGLEYTLQKMEEYKNKAIEELNGFAEGELKQSMILFAEFMSNRDY"
     gene            53914..56781
                     /gene="polA"
                     /locus_tag="PLZ54_00200"
     CDS             53914..56781
                     /gene="polA"
                     /locus_tag="PLZ54_00200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOJ65392.1"
                     /translation="MKKLFLIDAHGLIYRSYYAFIRSPRINSKGLNTSAIFGFVNTLE
                     EVLKNEQPTHLAVVFDPEGKTFRHEAYEQYKAQREKTPEDIRKALPIIKELVAAYNIP
                     AIEVTGYEADDVIGTLAKQAEKKGFEVYLLTSDKDLGQLVSDHIFMFRPHHNGGFEVL
                     GPDQIKEKYQLENQQQVIDLLGLMGDSSDNIPGCPGIGEKTAVKLLQEFGSIDNLLQN
                     TDKLKGSLKTKIEENKDLINFSRFLATIKTDVPVALDEEALKVEPADSEKLRALFQEL
                     EFRTLLNNKFGNTFSSFPPDYPLLQTPSGNKKSSKAEQMNLFGETNRPQKTEPEKEKI
                     LSKPEENIEEISPVASNGFLALQNIPHRYQLIDDKVKRSYLVSQLFLQKSVCFDTETT
                     GLDVFSADLVGLSFCFSEGEAYFVTLPDNREEATKILNEFKAFFLSEQIEKIGHNMKF
                     DLLMLSNYGIDVKGKLFDTMIAHYLLQPELRHGMDYLAEIYLHYHTIHYEDLVGAKGK
                     NQLDIRNVEISKLTEYAAEDADVTFRLKQILEKELAANELEKLFYEIEMPLMKVLVIM
                     EKTGVRIDSEALRQSSEILTENMLKLEKEIYEMAGGEFNINSPVQVGEVLFDRLHLDD
                     KAKKTKTGQYSTSEDVLEKLRHKHPIVDKILEYRGLKKLLSTYIDALPQLIHPKTGKI
                     HTSYNQTNTATGRLSSSNPNLQNIPIRDELGKEIRKAFIPEENSLFLSADYSQIELRV
                     MAHLSNDQNMIEAFRNGLDIHAATAAKIYKVPLEQVTSEMRRQAKTANFGIIYGISAF
                     GLAERLGISRTEAKTLIDEYFLAYPKIKTYIESAIKKAKENGYVETLLGRKRFLPDIN
                     SQNSVVRGFAERNAVNAPIQGSAADIIKIAMVNIQRKLEKENLQAKMILQVHDELNFT
                     VPVNELETVRELVVYEMEHAFPLSVPLIVDCGVGNNWLEAH"
     gene            complement(57038..57988)
                     /gene="corA"
                     /locus_tag="PLZ54_00205"
     CDS             complement(57038..57988)
                     /gene="corA"
                     /locus_tag="PLZ54_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOJ65393.1"
                     /translation="MSELRIFYHENDGKLKISKSIDTLKKINLKDIIWIDMNDVSENI
                     ESELEQFLKIYIQEDEEIEEIEMSSRYIQTDDSIVANSNFLQDNYVMQPVSFIIKNGI
                     LVSVRNTELKSFNETVKKIFVNTSNFPSGYHVMVSLFETRVEYDADMIEDVTDQITNL
                     SKNLNTESDLDQDILLQIKELQEKVMIIRQNIVDKQRVVSNMLKCNFFPEDLIPRLNI
                     ILKDINSLFEYTRFDFDRLDYLQDTFLGLVNLQQNNIIKIFTVVSVIFLPPTLIASMY
                     GMNFKYMPELDWKFGYPFSILLMISFTVGVLVFFKRKKWL"
     gene            complement(58062..58844)
                     /gene="rsmA"
                     /locus_tag="PLZ54_00210"
     CDS             complement(58062..58844)
                     /gene="rsmA"
                     /locus_tag="PLZ54_00210"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOJ65394.1"
                     /translation="MTLVRPKKYLGQHFLKSMDVARRIAGSLPVGSPASVLEIGPGMG
                     MLTQFLLQQPQIDLKVVEIDHESVVYLQQHFPQLQILEKDFLQLDLNQIFSHPFSIIG
                     NFPYNISSQIFFKVLDNKDLIPSVVGMVQKEVAERLASPPGNKTYGILSVLLQANYDI
                     NYLFTVDETVFYPPPKVKSAVISLKRKENPEVDYDEQLFRRVVKTAFNQRRKTLRNSL
                     KSIIPSHSMYAEPLFDLRPEQLDVKTFVELTNKIGSALKEKQ"
     gene            59109..59552
                     /locus_tag="PLZ54_00215"
     CDS             59109..59552
                     /locus_tag="PLZ54_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOJ65395.1"
                     /translation="MPLQFFRYGVSGAANMVFDWVLYFVVYNFVLRHQMLHLGFITMS
                     SHIAALALTFPVSFMTGFLLQKYVTFSASYLRGHVQLRRYLLVVIINLGLNYAGLKFF
                     VEIAHFYPTPSKMLTTIITTLFSYFAQKKFTFKTASLSLNKESNH"
     gene            59586..60578
                     /locus_tag="PLZ54_00220"
     CDS             59586..60578
                     /locus_tag="PLZ54_00220"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOJ65396.1"
                     /translation="MAVTTKMTTDKSKLWNIFKWSCLTAAFLFLGYRLICFQHYGRLL
                     SQFKEISFWKTWYLWGVILLLPANWLLESVKWQRLVRNSEKINMALAFQSVMAGFLTA
                     FFTPNRVGELAGRIAYLQPENRKAGITLSFVNSITQNIIMAICGIPAAILFFLSNKNS
                     EFAGENNFFGYFLIVCGILLLLSGIYFSLPPLVGRIKQKKLSAKWINFIACLADYSFA
                     DLLKILLLSFLRYLVFSFQFYLMLRFFGVEINGLAAAIAIPTTYLFVTFTPSMAFSEA
                     AIRSSYSVLVIGAFSGQTVQIILASTFIWVINFVIPMFVGSFVMMQTHPLRSNK"
     gene            complement(60588..61436)
                     /locus_tag="PLZ54_00225"
     CDS             complement(60588..61436)
                     /locus_tag="PLZ54_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HOJ65397.1"
                     /translation="MKTIQNTAILVFLLAFNSILYGGERIKLTILHTNDTHSQIEPTE
                     KSNLPVSDKGGYARRMGIIEQIRQQNKNVLLFDAGDFWQGTPYFNFYNGRIEVDGMNK
                     MKYDAVTLGNHEFDNGLDTMATVLKNAQFPILTSNYQMDSTAMATLVKPYLIIRKAGL
                     KIGVMALNVNPKGLIFRNNYKKMNYIDPVETAINISNFLKRSEKCDLIICLSHLGADT
                     LKQTLTDYDIIRKTRYIDVMIGGHSHQIITQTNLKNAAGKPVVLAQMGKSGLYLGRID
                     LELEKK"
     gene            complement(61449..62204)
                     /locus_tag="PLZ54_00230"
     CDS             complement(61449..62204)
                     /locus_tag="PLZ54_00230"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF014871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOJ65398.1"
                     /translation="MKVNFCTLFVLFNILFYSCSPKTWQVMEATSVKIPIDSTTEKFA
                     DKEYEAALQPVKERIDAEMAVVIGYSAENMRSFAPESPLSNFCADVFRRAASDFLHQE
                     VDVSVVNLGGLRTEIPAGNITIRNIYELMPFENELVILWVRGDKLIQLFQFFASVGGE
                     GVSGISMKIKNGKATQILVGTNPIDPEKIYTIATSDYLAEGNDQMTALSEYEKISGTG
                     LKLRNVLIDFIRNETLKGHVIQSKIDGRISMEN"
     gene            complement(62360..63424)
                     /locus_tag="PLZ54_00235"
     CDS             complement(62360..63424)
                     /locus_tag="PLZ54_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HOJ65399.1"
                     /translation="MLQIFQPFRIKDITLKNRLGMSPMCQFSAQDGLANDWHLVHYGT
                     RGVGGIALIIFEATGVSPVGRISPEDLGLWKDEQIEPLKKIVDFIHQQDSKAGIQIAH
                     AGRKGSRSSPFKGEKQLTNKEGGWQTIAPSAIPFRPEEIPPHELTKSEIKKVVNQFKD
                     AALRAFQAGFDVLEIHAAHGYLIHEFLSPISNFRKDEYGGSFKGRIRLLQEIVSAVQT
                     VWPERLPLFVRLSATDWTDQGWCLEDTVELAKILYLEGVDLIDCSSGGILPNVHIPLA
                     PCYQVPFSEQIRKTGILTAAVGLITKHEEIQDILRTEKADIVLLGRELLRNPYFALHC
                     ARHVKENSKWPQQYLRAKLE"
     gene            63663..65135
                     /gene="rpoN"
                     /locus_tag="PLZ54_00240"
     CDS             63663..65135
                     /gene="rpoN"
                     /locus_tag="PLZ54_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOJ65400.1"
                     /translation="MLKQQLQQKLQQKLSPAQIQVIKMLEIPTLELEERIRQELEENP
                     VLEIGLEPEEEKKELDDFGEDYEETGNDEGESNDEIDFEEYMSDDDIPDYKLKANNTS
                     KDDKYEEIPFSAGSTFHDFLMEQLNLVPLSERDRALAEFVIGNIDEEGYLRRTPEALA
                     DDVAFQANMDTNVKEIEKIVKIIQQFDPPGVGASSLQECLLLQLEKKRNEKEIEIPKR
                     IIQDYFEEFSKKHYDKIINSLEIDGNTLKNAINEIIRLNPKPGNAWSGNILEKNMSII
                     VPDFIVENNDGELTVHLNNSNIPYLRVNAAYSEMFQDYTSNKENQTPQMKEAILFVKQ
                     KIDSARWFIDAIKQRQQTLLTTMTAIVEFQKDFFIEGDEIYLKPMILKNIADRTGYDI
                     STISRVSNNKYVQTEFGVFPLKYFFSEAMTNDAGEEISTREIKKILQECVNNEDKRHP
                     LNDEMLAEVLKQKGYLIARRTVAKYREQLNIPEARLRKEI"
     gene            65173..65778
                     /locus_tag="PLZ54_00245"
     CDS             65173..65778
                     /locus_tag="PLZ54_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65401.1"
                     /translation="MKILYKIISIILQPILLPTYGILLLIQIPVFANLPMQWKAIAVG
                     GTFLFTTLLPSIPVVIMYFRKEVSDLFISNREERTLPYLFSFLAYVFWTLFLVRTMHL
                     PMFIGGLGIGSSISILLITFINVKWKISAHLASLGGLAGGIFGICYRYSINPLWLFIL
                     ILLLAALSAISRVALKAHTPMQTIAGFALGFLCVFLPVLLL"
     gene            complement(65801..67840)
                     /locus_tag="PLZ54_00250"
     CDS             complement(65801..67840)
                     /locus_tag="PLZ54_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ65402.1"
                     /translation="MHKNFFFYLIEILFLFFFIPVRAEILFQDMGMDTAKTYSIPEIV
                     VVEKFQNSEIRSAMPLQILSAKKLENLNVLQLSDAIKYFAGVNVKDYGGIGGLKTVSV
                     RSLGAEHTAVSYDGIMLTDYQTGQIDLGRFSLDNVDMVSLNNGQSDNIFLPARSFASS
                     SVLNIRTLQPRFEEEKSIHGKITCKVGSFGLINPSLFIRQQMSRKISAVFSGEWLTAE
                     GEYPYLLIYGTQTGDSSSIETRKNTDVETLRLEGTIFANFFHRGSGYLKTYFYESERG
                     LPGAVIYYMNENTNHQRSKDQTFFTQAHYESSFSSLLSFQANTKYQNGYLHYVDPNYN
                     NSGGKIDNTYSQQEFYFSASFLYKAFENLSFSASTDYSLNTLNANLPEFAFPKRNTVL
                     MVLNAKYVSNQFLATAGGLQTFVNEKVKFGSSPNDKKHFSPFFSLSVKPFEQYDFRIR
                     SFYKNVFRMPTFNDLYYSYVGNRNLEPENAQQFNAGITWSFSSENISLLSVTLDAFHN
                     AIRDKIVAFPNKDIFNWTMKNCGKVSINGLDVAMESGISITRNIGLIFGGSYTYQQAL
                     NVTDPNAEDYLHQIPYTPRISGSGKAALEMPWVNLSYSFIWSGHRYTLFQNYASNRLP
                     GYADHNLSLSRSFKKSSYSCFFKLEINNFLNKNYEIVRGFPMPGRSFRSIVAIEF"
     regulatory      complement(67941..68181)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT        21800 a        12653 c        12954 g        21030 t
ORIGIN      
        1 atcagacctt taaaaaataa attatattct gatgaaaatt gtcaaaaaac tttttaaata
       61 tttttcctcc tttttatgtc aatgtcttaa aataaccaat tctctttgag taacttttcg
      121 gaggagattc atataataaa acttgcaaat atatacactt ttttaattta ttttattgat
      181 aatcttttgt ttatacgctt tttaagaaca gactgtttac aatactacct caatgagttc
      241 ttttacaaat ttaaccgacg ttatttcggt gaagctatct tcgacacatt gcttgtagct
      301 gctgtaagct acaactccga tttcaaacca agaatttaca ataggaatct ttgtggataa
      361 tcataattta gaagtgaaaa aacatatatt acaagtttta aattgaaaca ttataacatt
      421 ctcactatta aattgatgaa tagaaaaact caaattacat ttgattattc ttgaacctaa
      481 ttaaggtacg ttttattttt tttcgtcaat attttatatt cgtaagtcta gatgtggtgc
      541 gaattctaat tcatagatgt atgcctatct ttttatgaat tgatatgttt ctcaagcagt
      601 tttatttaaa tagtcgaatt aaatgattaa atttcaatca atattaaatg taatgcaatg
      661 aattatttct tttttttatt taaacgtttt tgtaaaaaaa atagtgtaaa gcttatcgta
      721 tcaataattt tattgttgat gttaaatgta aatgcagctt atgcatgtca aaccattaat
      781 gaaggttggc aatttgtaaa aacacccgga aataaaacag taattccaac tttcgaaaat
      841 gccgaaaaag tccatctccc gcacacatgg aacatggccg atgtgtggga tgacgtaaag
      901 ggatattatc gcgatgctgc ttggtatcgt aaatcaatac ttgtagctga ttcgttgaaa
      961 aacaaaaaaa tattcttatt ttttgaagga gcaaatcagg aggctgccgt ttacgtaaac
     1021 ggacaaaaag cgggtgaaca taaaggagga tatacagctt tcagagtgga tattactaac
     1081 cttgttgatt tcgggaaatc gaatcggatc gacgtaatgg ttgacaacag ttattctact
     1141 gaaattgccc ccatatcggg cgatttcagt atgatgggtg gcatttaccg tgatgtttat
     1201 ttagaagtaa gcgaaaaaat acatttcgat aaaatgaatt atgccggtca gggagtattt
     1261 gtgaaaagct ttttagatgg cgcaaatgca ggttttcaga tagacgccaa catagtgaat
     1321 agttccggcg aaacgaaaaa gctcatgttg cggacccgta ttaccgatca gaaaggtaaa
     1381 attatttcag cgcaaatcag taaaagtatt aatctgaaaa acgctatgca gcaaaatgtt
     1441 aagcagctgt ttgcaattcc tgattatcag ctttggtcac ccgacaatcc gtatttgtac
     1501 accgttgaat gtgaaatttt ggatgctaaa accaagaccg ttttagacac ttactgtgtg
     1561 cgttcgggta ttcgtaccat tgccaaagac gaaaagggaa atattctgct caacggcaaa
     1621 gtaataaaat tgattggcgt aaatcgtcat caggattttg atgggttaag caatgcgctg
     1681 cctgacgcta ttcatcgtcg tgatgtagaa ataataaaag agataggggc taatttttta
     1741 cgtacctcac attatccgca cgaccctgcc atttacgatg cctgcgatga gttgggtatc
     1801 ttagtttgga gtgaaatttc ggttgtgaac caaatcggaa taaatgagca ttttgaacaa
     1861 aatacactaa acaatatgaa ggaaatgatt ttacaacact tcaatcatcc ttcggttgta
     1921 ttttggggat ttatgaatga agtgttgttg gccgcaaact cttttcctga aaatgaacgg
     1981 ggaaatttat ttgttaaaac ccgtgaactg gcacagaaac tgaacgatgt agcaaaatca
     2041 ttggataaca cacgacttac aaccatagct catcatggcg attttgagaa atatgaaaaa
     2101 gccggcttga ataatatcac cgatgtgtgc ggttacaatt tgtattttgg ctggtatgta
     2161 aatggatttg gtggatttga tgattttgta gataatttca aacaaaaata tccgacaaag
     2221 cctctaatta tcagcgaata cggtgcgggt tctaataaag gaatttatgc ccgtttgcca
     2281 cgccgctttg ataactcgat gcagtgggct gaaaaactac acgaaattta tttggataaa
     2341 attatacgga atcctaaaat tgccggcagt tctttatgga attttgccga tttcagttcg
     2401 gaaggccgaa aagataccga cccgcatatg aataacaaag gtttgttgta ttatgaccgc
     2461 actcccaaag atgtttttta tctgtataag gccaaattgc aaaacgaacc ggcgctgaaa
     2521 attgctgccg gaaaatggct gaagcgaccg gttgaagtgc ctgtggaagc aagaactttt
     2581 accgatacca ttaaaattta cagtaattgt cagcaggtta cacttcaact caatggcaaa
     2641 acggttggca attttcaacc cgatagtata tgtcggattt ttgctccgct taatttcgta
     2701 aatggtaaga atcagttggt agcaattggc aggtcgacaa atggaaaaac aattacagat
     2761 gtggtcaatt ttgattatca ctttattcca tttccgctta acaacaattc atttcgggaa
     2821 ttatgcgtca atgtgggcgg tcatttcgat tatgtggata cgaaaagtaa cgaagtgtgg
     2881 atgcccgaca gaccttttcg ccccggtggt tggggttatg tgggcggcga agagtttaag
     2941 atctggaaag gacagcgcac cggtggcgaa cgggtgattt ataataccat taacgatccg
     3001 ctttatcaaa cgcagcgggt tggtctcacc gaatatcgct tcgatgtggc ggcaggttgg
     3061 tacgaggtgg aattgctttt cgccgagctg caaagtgcta aagagctgaa agatgcattt
     3121 ctgaataatc tgggtaacga aaccaaaacc gagctgttga aagatggtcg gatttttaat
     3181 gtaagtatta ataatcaatt ggttttaagt gagttgaatt tattggaaag tgtaggtgaa
     3241 aatacagcag ccaattttac tttcagggtg aaagctgctg ataatggtgt ggttgtgaaa
     3301 tttgacaaaa tacgttccga aaccgtattg aatggtatta aaatcagaaa attttagaaa
     3361 tgagaaagtt tttttatgtg ctgatagcct gcttattggt cgctgatctg tcgttttcga
     3421 aagagctgtt ttacattaca aagttcggtg caaaggctga tagcaaaacc aacaatacca
     3481 aagccataca aaaagcaatc gatgcagcag ctgtgaaagg tggaatagta atagtgccgg
     3541 ccggtgaatt tttgagtggt actatttttc tcaaaagcaa tactgaattg catttaatgc
     3601 aaggcgcaaa acttattgct tcgcctcgta tcgaagatta tgcgcacctt tcctggggac
     3661 atcacgaaga tcgtactccc tggcatttaa ttgttgccaa agatcaaaaa aatgtaacga
     3721 taagtggtaa aggtgagatt tatggaaacg gacctgctta cagggaccct aatcgaaaaa
     3781 acgaattttc tttttttcgt gaaatagagt atcgaccttc cccgttaatt gaaattcagg
     3841 gatgccaaca ggtgcaaatt aaggatattc tcatcaccga atcgccgggg tggactatac
     3901 acctgtacga cactgacaat gccaaagtgc gtggaataac catcgaaaat tcgttgtatg
     3961 gacccaatag tgatggaata gacattactg gaagtcatga agtaattatc agcgattgta
     4021 acataaaatc aggcgacgat gcaatagctg tgaagaccac cgaagattca cgtagttgtg
     4081 aacgaatagt agttacaaat tgcgtgtttg aaacgaattg tgtggccgtt cgtattggtt
     4141 tcgaaagccg taaagatatt tcggatatta cggtaagcaa ttgtgtgata aaaaatacct
     4201 cgcgtgccat tgatgttcgt acagtggaag gcggaaatat ccgcaatgtt tttgtttcgg
     4261 gcataaccgg aaaaattgac tcgggttggg ctgtggacag gattattgaa gtacattcga
     4321 accgtattga caatccatac gatattgcta taaaggagca tcccaacttc gggaaagcaa
     4381 aaccggttga aaaagccgga gtaattgaaa atattcattt ttcgaatatg catttccgta
     4441 cttcggggcg aattttggta agtgccattt ccgaaggact gattgacaat attagtttcg
     4501 agaatattaa cctcacctac aatctgttgg aagatgcacg agaaattggg aaagtggcac
     4561 aatcggttgg ttttgcccgt aatttaccgt ttgtgcgttc gcaaaatgcc acatttgttg
     4621 ttgaaaatgc agctaatgtt cgtattcaaa actacacagt taaatggcct gtatatcctg
     4681 ttccggacga ttgtttgttg ttaaccagtc cgaatcgctt gtataataaa tggtttttcg
     4741 atggaaatga agcaaaaatt aaaagtggtg aaatgcgtgt aaaatacgat gtctttgctt
     4801 ttagaaatgc caaaaatatt cagttacaaa acacaacatc agtaaattct gaaaatggtg
     4861 ctgagtattt ttcatcggaa aattccatta tattaaagta aaataacgtt ttttctttgt
     4921 gaaactttgc ataacccttt gtgatacttt gtgttataaa ctgttatttc acaaaacaaa
     4981 caaaactgat tcaaaaaaca aaaagaaatt atcttaaaac catacaaaca aatgaaacga
     5041 aattttgcac tactttttat tctttcaata tttatttttt cagctaatgc tatcgaaatt
     5101 aatgtattga acgaaggcgt tgtcagtgat tcaaatttag ttcaaactgc taaaattcag
     5161 caattaatag ataaatgcga caaagccgga ggtggcaaaa ttatatttcc tgcgggagct
     5221 tacaaaaccg gtagtttggt gcttaaaagt aatatcactt ttcgtgtcga aacgggagct
     5281 acaataattg ccagtcgcga taccaatgat tataaagcag tacaaaacaa cagtggtgat
     5341 attatccctt gtttgttgta tgcgaaagat gcacaaaaca ttagcattga gggaaatggt
     5401 gtgattgatg gtaaggcaac acactattgg ggcgatttgg acatggtgga tggatttatc
     5461 aaagatatta ctgaaaatgc acaaaaagcc ggagtgccaa tgaaacgcta ctatcatatc
     5521 aagccctata cgtgtatgat ttatttcgag aattgcaaat ttgtttctat tcgggatata
     5581 acactaaacg catctcagtt ttggactttg catgcacgct ggaccgatta tatgaccatt
     5641 tcaggtgtga aagttttttc tgatttggaa aaaggaatta atgccgatgg aattgatatc
     5701 gatggttgtc gaaacgttac gatttcagat tgcattgtgg aaacaggtga tgatgccatt
     5761 gtgttgaaaa cgacaaattt acatggagag tcccgttcgt gcgaaaacgt taccgtgaac
     5821 aattgtgtgc ttcagtcttc ttcaactgca ttgaaattag gaacagaaag tcatgccgat
     5881 ttccggtata tttcgtttaa taattgtgtt attcgaaatt caaaccgtgg tttaagcatt
     5941 gtaattcgcg atggtggaac tgctgaaaat atacagtttt caaatataat ggtcgaatgc
     6001 tcgcgtcgcc atttcaactg gtggggaaat gctgacccca tctggttggt tgtgctgaaa
     6061 cgccgaccgg attcccgcgt tggtagtatt cgtaacatta cattcaacaa cattatagcg
     6121 catggtatgg gaacttcaaa aatcgaagga tttgaagata aaccattgga aaatatccga
     6181 atgaacaatg tgcagttttt tatgcatcct gaaaactaca tagataaacg tgctgatgat
     6241 gctttcagag ctgtaaatgt gaatgatttg tggcttacag atgtgtcggt aaaatgggat
     6301 accacatcgg tagagccgtt gtggcgcaat gcttttactt ttgaaaaagt aaatgtatta
     6361 gtgctcgaca aactctccgg tgaagaagcc ccaacagcaa aaggctcatt tatagaactt
     6421 cgcaacacag acaacgtact gattgaaaac tgtattacaa ataaagagtc gaatgtattt
     6481 ttgaaatcag tttcaaaaac aaacaaaagg gttagtgtag gtaaaaacat tggaattgga
     6541 aaatcaaaac taactattct aaaaaaataa aacatgccaa aattacatac gatatcattt
     6601 attgttacat tgtttcttgt ttctacggtt tttgggaaaa aaacaacttc tgttgatatt
     6661 tgcatttacg gtgcaaattc cggtggtgta attgcagcct acactgcgca aaaatcgggc
     6721 aaaagcgttt tgctcattga gccatcttca aacttgggcg gattatcgac ggacggattg
     6781 ggatataccg acattggcaa taaatatgtg gttaaaggac tttcgcgtga tttttatcgt
     6841 cgcttaggaa ctcactacgg caaacttgaa caatggatat ttgaaccatc ggtagccgat
     6901 ggaattttta atgattatgt aaaagaagca gatctgaacg tgttgtataa catgagatta
     6961 aacaaggtca acaaatctaa gaacacaatc caatccattg ttatagaaaa ctcaattaaa
     7021 ccttccaaag caacgaatca aaccatcagc gccaaaatat ttatcgattg cagttacgaa
     7081 ggcgatttga tggcaagagc aggtgcttca tatacaatag gccgcgaaag caacacgctt
     7141 tacaatgaaa catataatgg ggttcaactt cttgatgcac atcagtttcc cgatggtatt
     7201 gatccctaca aagagaaggg aaatccaact agtggattac tttggggcat atcgcccaac
     7261 ccggttttac ccaatggtac cggtgataaa aaagtacaag catacaatta tcggatttgc
     7321 cttaccgaca agccatcaaa caaagtagaa atcacaaaac cagataatta ccagcctgaa
     7381 aaatatgaat tgcttatccg gctaaaagaa aagcaaccat ggaattcgct caacgatatt
     7441 ttcatttggt cgatgatgcc caatcgaaaa acagatatta ataaccgtgg ggggttctct
     7501 accgacatga ttggcatgaa ttggaattat gccgatgcaa gttatgacga acggaaaaga
     7561 attattaaag aacacgaaga ttatacaaaa ggcttactgt atttcgtagg acatgacgag
     7621 cgaatcccac ttaaaatccg taacgagatg ttgaaatggg gttatcctaa ggacgaatac
     7681 ataggaaata gcaactggtc acctcagctt tatgtgcgtg aagcccgtcg gctcgttggc
     7741 gaaaaggtta tgacacagca ccattgccaa ggaaaagaaa tagtttctga ccctatagca
     7801 tgggcagcat ataccatgga ttcgcacaac tgcgaccgtc atgtagtgaa tggaatggta
     7861 aaaaatgaag gtaatgttga agtgggtggc ttcccacctt accccatttc gtatcaatcg
     7921 cttgtaccaa aaagaaagga agccagcaat ctaatagttc cggtttgtat gtctgcttca
     7981 cacattgcat acggttctat tcgtatggaa ccagtattta tggttatgtc ccagtcggca
     8041 gccattgaag cctgtcaggc tatagatgca aaagtgggtg ttcaggatgt cgatgcaaac
     8101 aaagtactta gcgaacagcc gatgctgtat tatttatccc tgtaaaagat taaaatttat
     8161 ttttcagttg ttttaataac taacttacgt atttttaaat catgaataaa acatttttgc
     8221 ttatctgcct ttttttattt cagttgttta attatagccc tttgtctgcc aaaacaaagt
     8281 ttccagcgta taaagaccct tcgcaaccaa ttgataatcg cgtaaaagac ttactgaagc
     8341 gaatgacact ggaagagaaa attggccaaa tgtcacaaat cagttcgagt gaattgaacg
     8401 acagcaaacc aggtgacccc aagtcgcgtc gtttccgtcc gtatttagac cccgaaaaag
     8461 cgaagaaatt aattaaagat tatcatatag gttcttttct ggttggcttt gccgtagagc
     8521 ctgagcaatg gtataaattc acttatgagc tacaatccat agccatccaa aattcacggt
     8581 tgggtattcc aatgatttat ggcaacgatc atattcatgg tgccaactac gtaattggcg
     8641 ctacaatttt tcctcaggct ttaggtttgg gatgtacatt taataagagt ttggctgcca
     8701 aaatgggcga agttactgca ttggaaatag ctgatttagg gcagccatgg aactttgccc
     8761 ctgttcttga tctggctcgc aacccttact ggccacgcat gtacgagtct tttggcgaat
     8821 caacgcattt gatttcggaa atgggaacct cctatattaa agctatgcaa accgcacaag
     8881 cagctttgcc atataaaatt gcagctacag caaaacattt tattggctat ggcgaatcca
     8941 agactgggtg ggacagaaca ccggctgtga taagtgatca ggaattatac gaatatcacg
     9001 tgccaccatt ccgtgaagct attaaagcag gcgtaaaaac ctttatgata aatagctcgg
     9061 aagtaaacgg tgtacctgtt catgcctcgc aacgtttgat aaatgggtta ctgcgtaaag
     9121 aattaggatt cgatggagta gttgtaacag actgggctga tattcttcag ttaattggcc
     9181 aacataaggt tgcacgcgat gaacgcgagg ctaccaaaat ggctttacta gcaggtattg
     9241 atatgagtat gatagctaca caaactactt tctgcgacgt tgtcaaggat ttggttttat
     9301 ccaaagaagt gccaatgagt gtgatagata aaagtgtgtc tcgtattcta aaattgaagt
     9361 ttgaacttgg gttgttcgag aacccttatc cacgcaatga tagatttaag cgtataggac
     9421 ttcctgaaaa caacttggct gcattacagg cagccaccga aagtatggtt ttgttacaaa
     9481 acgatggagt gttaccactg aaagatatta aaaatatctt ggcggttggt ccaacagctc
     9541 actctaaacg aaatatctgt ggagcatgga cattggaatg gggtggtgct cccgaagaaa
     9601 tgtttcccgc cgatatggaa actgttttta ccgctttgtt aaaaaaatat ccgagtgcag
     9661 ttgttgatac aatttctaat ccattaatta acttaagtgc atttcaaaac aaagctcaaa
     9721 tatccgatat cattattgtg gcggtaggtg aggaacctca tgccgaaggt cgtggcaata
     9781 ttgaagattt gacattgcct gttgaccaac ttgaaataat aaaaattgta caagccactg
     9841 gtcgcaaaca cattctttcc attttcagtg gtcgaccacg tgtgattact tcagtggcag
     9901 aaaaagcaaa tgctattatt tgggcaggat tacccggtta tgaaggtgcc actgctttag
     9961 caaatattct caacggtgat gccaatccat cgggtaaatt atgttttacc tatccgcgtt
    10021 cggtagggca tataacgcca tataatgtaa aagtacacga cacatacact tataaatggc
    10081 cattcggaca tggtttatct tattcaaaat atgaatacgg aaatcttata ttatccgact
    10141 ctatcgttaa acccgggcag actttgaaaa ttagcgtgca ggttacaaat aaatctgaag
    10201 tcggtggcag cgaagctgtt ttatggtatt tgagcgatgt ggttcgaagc atctcgcctg
    10261 cacgcaccaa tttagtgggt tttgataaaa tttatttgga gccaggcgaa tcaaaaaccg
    10321 taaattttga cataacacct gaaaaacaat tgacatttcc aaacgaaaaa ggcgaaatgc
    10381 tattggaaga tggtgagttt gttataaagg tgggtgataa aaagcaatcc ttttacttta
    10441 caaaatctgg tcatactaca ccgaaatcag gaaaagaagg tcgttcgcac ttacaggagg
    10501 aaatctgacg ttcatttaaa cagaatattt gttaataaaa aaatttttat gcgaaaaaag
    10561 tttgttttgt ttgctacggt agttactttg ttttcaacaa ctccaacagc ttatcccaat
    10621 atatataaac caacgcaact gggtgctatc cctgatggta aaaccttaaa caccgctgta
    10681 attcagcaag ctattgataa agctaatagt catggcggcg gtactgtact tttagatagt
    10741 ggtgtattcg tttcgggaac aatattttta aagtcgaatg taacgctgca tattgctgcc
    10801 ggagctactt tgcttggctc accacgtatc gaggattata ccgaacttac ctggggacat
    10861 aatgtcgatc gtcagcccta tcatctgata gtagccgata atgcacaaaa catcacaatt
    10921 gagggaaaag ggactattga cggaaatggc gagtattttt gggaagaata tgaacgcaat
    10981 gccgatggca atatggttat accccgttgg atcaaagcta aaaaactcaa ggtgagcccg
    11041 ctaattgaaa ttacacgctc gcaaaatatt tatatccacg atgtaacaat taaaacaggt
    11101 ggtggttgga acctgcatct gttcgattgc gatgtggcga aaattcacgg agtaaacatc
    11161 atcaacaatt tgtacagccc aaattccgat ggcatagata ttactggttg caacgatata
    11221 ataatttcgg attgttacat aaaaacatgc gacgacgcca tttgcctgaa aacaactccc
    11281 gacagccgtt catgccaccg tgttacggta acaaattgtg tgattgagac cttgtgtgta
    11341 gcattgaaat taggatgtac cgaatcgagt aaagacatta ccgatgttac gtttaataat
    11401 tgcgtagtaa ataaatcatc gcgtgccgta atgattgggg tgcgagatgg tgccacttac
    11461 gaccgcattg ctattattaa cattgttgcc aatactaatg caccattggt ttttaatcgc
    11521 ccaattcaaa ttttagtgaa taaattaaat ccagacagca aagcaggtac agttcgcaat
    11581 gtattgatta gcaattttat atgcgaaacc gaaggacgta ttttactcac aagtgccgag
    11641 ggaggattga tagagaatgt aattttacgc gatatcgttt tgcgctatcc tatgattgaa
    11701 agccctcagc gatatattgg tgccaaagta agttcacaat tcccaaaacc aaccgatcat
    11761 cccgaagctg taactgcgaa tgctgcttta gtggccgaaa atattaaaaa cctggtaatt
    11821 gaaaatatga tagtcgattg gcctcttact gactcaactc caatcaattg gcaacacccc
    11881 gaaaggatag aaaacggcac atcgcacatt cataaacccg actatacgaa agcgcggcaa
    11941 accgagttca gcgctatttg ggcacgaaac gtacaaggtg gttatttccg aacacctttg
    12001 ctcaaatctt catcgaaaac aaaaccggta tttgatatca aaaaatccaa tatcaaaatt
    12061 ctgtaatctt tataaattca attaaaacga catcaaatac acgtacaaat gaagaagtta
    12121 ctatttattc tgatactcat acctattctg ttaccagctc aaaaaaatga tgaattttta
    12181 catgcacttt acgataaatt gcatgattcg cctatgcagc aaaaaatacg tgaaattgcg
    12241 ccaatgcctt ttggggtggt ttttctaccc tgggcaggtt gtaccgagca agatatgcgt
    12301 caacacttcc gcatgatgaa aaggctgggt tttcataacc tgaagcaaac catggctaca
    12361 cctgaatggt ctgaaaaaga gatattgcga atagccttgg aagaagatgt tattcctttt
    12421 tggtatggag aaggaggctg ggagcctatc acaacagact tattacgtaa actcaacatt
    12481 ccgcttagtg ccacaaaaga agaaatccgt aaaaatccga aaatgcttga ataccaaaag
    12541 aatgtttttc aaaagcaact tgccgattgg aaaaagccca tttctgatac aactctgata
    12601 ccctacaggc atgaaccgga tgcatacctt cgaccatctg atataaacct ttttaaacaa
    12661 tgggtaaaaa acaaatatca atcgattgat cagcttgtaa cggcgtggaa tcaatacgaa
    12721 gttggaataa aaaaaatacc atatcgttcc tggaacgact ttgaaaatga taagtttctt
    12781 tttagtgctc ctagtaatga tgaaattatt ccctcttcag gtcgtgaata tggttttgta
    12841 aaggatgtat tgcgttttaa agcggatatg tatctaaaaa atgtaaagaa ctttgttgtc
    12901 tccaatcatc ctgaacaacc ggtaagagct ggaggtgaga tggggttatt ccttcctttt
    12961 gcatcgcgtg ccatcgatat ggagggtatt gccgatgtga tgaaagaccg gggctctttt
    13021 tacccatcca ttcatttggc ctggcatttc gaagaggtgg attacgaggt tacacgatgt
    13081 gtttatatgc aatcgtcatt agctaccgat tggtttaaag ggggttgggc agctgtatgg
    13141 gagtctacag gtggacccca gcagtttagt ggaggtaaag gttgggatcg caaaggagct
    13201 gaatcaacag ccggttttac ggtcgatggt ggtaccatta cacaaatgct tctttcatat
    13261 ttagccggag gactcaaagg agtgggtcta tgggcgtgga attacaggag agcaggttgg
    13321 gaatcgggcg aatatgcttt gctcaaccgc cagaacgaac cgggcgaaag agcaattcgg
    13381 gcaggacaaa tagcacaggc cgctgaaaaa taccgtgatg aactttgggc agcacacaaa
    13441 gaaccttatg tgggtgtttt tgtaaattgg gacaatgaag ccatttgggc tgcttgtgcc
    13501 gtaccaaacc gcacacattt caaaaattat ccggttcagg ctcgtattgg tataagtaga
    13561 gcacttatca atgccaacat accttgggaa catgttacgc cttccgactt aaaagcggga
    13621 ttggctatgc gttacaaagt aatctatctt ccggcacaag tggcaatcaa tgaagattta
    13681 ttcccgattt tcgagcaata tgttcgcgat ggcggccgat tggtacttga tgcgcccgga
    13741 gcatggtgga acgagcaagg caaagtgctc gataccggaa aaggttctgc atttgaacgt
    13801 atttttggtg cttctatcgc cgattatcaa tatgctaaca atgtaaagcc ccaattggca
    13861 aacctaaacc tcacgggttt tttactcgat attaaaccca catctgctga tgttttacaa
    13921 aaatttaaca acaatgcagc ggctgttaca gaacatagac tcgacaaagg ttcagcaatt
    13981 attttatcac ccgatgcatc tttttctatg aaaaacaaag gaaatgaacc aatggaaaat
    14041 tgggtaataa atgtaataat gcaagacata aggaagccgt atgaatgtaa aaatgccatt
    14101 gtgtatagat tggggagcga aatggctgac cattacttta tattgaatga tggcagtgcc
    14161 aaaaatgtat ctttgaaact caaccgttat aaataccgcc atgctatcga tgcggttaca
    14221 caacaaaaag tttcaatctc atctataaat atcgaagcca atggtgggcg ttggattcga
    14281 tgcgaaaagt aaaaaataat tatcataatg tgtaaagtcg tttttttata aataattttt
    14341 atgagtaaat tttcatggtt cattgttgta tttttgtttc tgtcctcatg caagtcgtta
    14401 agtggatact atgatgttac agattttgga gctaaaaacg actctaccaa attgtcgacc
    14461 aaagccatac aaaaggcaat tgataaggca aatgcaaatg gcggaggtaa agttgttttt
    14521 ccacaagggg tatatcttac aggaactatt gttctgaagg ataatgttac gctttacttc
    14581 aatgagggat cgcgcctgaa agggtcagac aagcatgccg attacctttt tcacaaaaca
    14641 aaaatcagtg tttatccaaa tccaaaccgt gcatacctga tatacgcaga tggtgtaaaa
    14701 aatatatcga tagaaggaaa aggggtgatt gatggtaatg gcacaaaatt ttgggagcct
    14761 tttgatacat taccccgctg gataaaacca ttaaatgggc gggtaagcaa tatgattgaa
    14821 atcaacaatt cgaagaatat cgttattaaa ggagtaacat tgagcaatag cccagagtgg
    14881 acatgtcatt tgtttgattg tgatgatgtt ttagtagaag gaatacgttt ggtaaataat
    14941 ttatacggac ccaataacga tggcattgat atatcgggct gtaacaatgt aatgatagct
    15001 aactcgtata tcgaaacttg cgatgatgca gtatgcatta aaaccttccc aagtagtagg
    15061 gagtcgagta atattaccgt tacaaattgt ataatgaaaa caacttgcgt cgctttaaaa
    15121 ttgggcgaaa attttaaacg cattgcagat gttacattta gcaactgcat tatttatgaa
    15181 tcgtcaaggg caattggaat ttatggcgat tgcggaggaa cgacggaaaa catcaatata
    15241 tccaatattg tatgtaatac caatgcgcca ctgatattga accgtccgat tcaaattggc
    15301 atttggaaag ggaaaggtaa tcccggtaaa atcagaaata tttcaatcag caattttacg
    15361 gctactaccg aaggacgaat tatgttaacg gcaattgaag gttcactaat tgaaaacatc
    15421 accttgcggg atgttaattt ggtatatcct atgatagaag acccaagcaa atatgccgaa
    15481 ggaacaacca gtaatcagtt ttcgtcaatt gatattgatg ctaaaaaggc aaaagctgct
    15541 attgtagcac aaaatgttaa tgggctggta attcacaatt tgaatattac atggccacac
    15601 gatacaatac ctactcaatg gaaattcgta gaacgtatcg aaaatggcag caatcgcgtg
    15661 catcgaccgg attatgcaac cccaaaagcc actgaattta gtatagtttg ggctaaaaat
    15721 gtcgattcgg gatatattta tgcaccttta gcgaaaccat cggctcataa tgtaaaagat
    15781 tatctgctaa taaatagtaa tattaagata aaacaaaccg atactataag gtatgatgaa
    15841 aaacctctct aaaaaataac aattgaagaa tgagattttt atttatcaca ttgctcttta
    15901 gcaattttgt tttaagtagt atttgtcagc aaaaccatac gcttcatccc gataaacgtt
    15961 ggtttaagga tgcaaaatac ggtgtttttg ttcattggac tttaaatgtg tcgcacaagg
    16021 atcgtgaaag aatgccctac gaagagtttc aaattaagtg ttttgatgaa gctaaaaaat
    16081 ttactgctaa taactatgac cctaaactat gggctaaaat gtttaaatca tggggcgcta
    16141 aatatgtagt tctaacaact aagcatcact tgggatttgc cctgtacgat tcggaataca
    16201 atcaattcac ggctctgaaa agcactcctg cgaaccgaga tttagtaaga gaattttgcg
    16261 atgccatgcg ttacgaggga ataaaagtag ggttgtactt tagtttacct gataaattta
    16321 attatgatta tcccacactc gttacacaaa acaatggtaa agaagtgatt tggcaacaaa
    16381 gtgattttgt tgcatggaaa cgcttcaccg acaacatgct gaatgaaata aaacatttat
    16441 gtaccaatta tggccgtatc gatctatttt ggttcgatgg cgactgggag cgcaacgctg
    16501 atatgtggcg aagcaaagaa attgtcgata cgattaacaa gtatcaaccc tgggctgtat
    16561 tgaataatcg attacgaagt gcaaatattg gtgatttttc aacgcctgag atggtaattc
    16621 caatagataa acgcaatggc atgtgggagc tgtgtactac cttgggatac aattgggatg
    16681 gtcctgagag tcaaaaaagt ataaaaaaac catcagaaac agtccgtatt ttgggagatg
    16741 ttattactca gggagggaat ctgctactaa acatttcgcc tgattatagc ggtagcattt
    16801 cacaacctca aataaaatcg atggaacttc taggaaaatg gataacggaa catgccgaag
    16861 caatttatga taccgaacaa ggcttaccat tgggtttgtt taatggtggc tctacccgaa
    16921 gaggttcaac catttatctt attgcttacg aaacagctcc ggaattggtt ttgaaaaata
    16981 ttgaaggtga aattgagagc attgtccatt taaaatcggg gaaacatcta acttggcgta
    17041 atatggatga ttatcacagc gataacaacc gcaaaggctg gaggtttatc agcttgccaa
    17101 ccaatttaat tgaaccatac gctactgtac taaaaattac ttttaaggat aagaaagtaa
    17161 aagtaaaaaa acatgacgga acttatttgg agtggaaaga atgataaaaa acatgtcgaa
    17221 tagaggattt ataataaaat aatgacctgt aaataaagta tttgtggcca aagcatcaga
    17281 gtttgttcag tattaacatc tgggttttga ctgtttctct caattaacat attcttttct
    17341 cggctacaac gacactggta gtccctgaac gattcttttt cttacgtaca aacatacgca
    17401 aatgtaatac tttttcctct tgcgtcaccc atttcggtga tgcgaaaaat acaaatatcc
    17461 cattgtgtga tcgttacaaa atacctcttg aatcgtgtcg aagtcaggaa gttgctgaaa
    17521 tttacaaagc gttaaagtac aaacaacagc tattcaaacg acgaaaaatt tgtagtaccc
    17581 aaactcatcc tccagacagt atatttactt gtttagcaat gagttccggc taatgaatgg
    17641 aaagttgggt taaatcagct gcttaatcta aaaaagaaag tgtccttttg gaacaccctc
    17701 ttttaatttt ttcacgcaaa gaacttattt ctaaaaaatt ttaatctcaa aaatcgataa
    17761 tgagtgtttt ggaagatgaa caatcagcgg cttatggttt ttaacttttt cagtcttatc
    17821 ttcaaaataa cccaattcgt ttgttatcat atcgtttgtt gtaaaaatgt gctgtttata
    17881 ttttgaatca gttttaaaat cagataaatc aatttccacg tccatatcat tcgcaaaatt
    17941 tcgattgatg aacgatatgt aaagcgtagt ttcgttgcgc gatgctaatg catcgacata
    18001 tgtcacccgg ggtacagctt cgattgagtt cattttatat ggctgtgtgt aaaacaactg
    18061 attcttttca ctcatctcca ataattggtt tccgtgatat tttgagtaaa gtgtggtaac
    18121 tttacccgtt gggaaaaatc gagccttcat ctttccggta gtgtccaccc gaatggcatt
    18181 aatatcccat acgttgccaa ccaacatcga ctgacaacca attttcatcc gtccggcatc
    18241 acgcatcatt gcatgtataa aaccagctgc acctattcct tttgcaaaaa gcgaatttaa
    18301 tgctgcatct ggtaagatct ttttgttcca ccaaccattc cagttccatt ctgttatagc
    18361 caaatcgtat ccggcatcgg ctgcatgatt atatttgcct gttttgggaa aataagattg
    18421 tcctgtttcc ggatgaatat aaggcgttgc cacccatgca ttccaaatat cagcttgggt
    18481 taatgatttt atatctattg tagtggtatc caattttacc gtattaattt cccatggtaa
    18541 ataataatga catgctaaat aactgatttt attgcccagt ctgcgtttta tttgttcaga
    18601 aacttctttt atttctccat cggtaataat cttgatattt gggtcaacgt caagcatcgc
    18661 attcacaatt gcctccaagc aattgacata atgatttacc aaactatcgt tcacatcttt
    18721 ttctttcatc gacttaaata attgaatctc attcccaatt tcgaaataac gaacattgta
    18781 aggcttcgtg tgaccgttct ttgccctgat tgctggccaa tcaggcatcc cttccgaaac
    18841 ttttgttccc accttgctat tgcaatatgc tacaaatccg gctgcatttt ttgccgattc
    18901 ctcaatactg attttcctga aaaatgcatc tccgatgttg accaccaaaa gaggttcgga
    18961 tttaagctct gaacaaagag ttaaaaacgc atccaaccca aggcgattat cggaagttat
    19021 cttgcctgtc ccagaataat ttcggtaagc cgggcgcttc tggtgttttc ccggtacatt
    19081 atctattaaa ttataccact gataataatc aacatcggta cctcccggat agcgtataat
    19141 cggaattttc atttccttta gataagccaa tacatccggt gaaacagaac ctgttttttt
    19201 atcaattgca ccatcgcctc ctatttcgcc gtcccagctg ggtttttcca taaattggcc
    19261 gaaaagttta tcatcaatat cagcgatgac ttttttacta attttaaatg ttggtaactg
    19321 accgaaagca aaaattgtgg aaaaaataaa tgcaagcaat actaattttt tcattaattt
    19381 aaagtgttaa ttattattac aagaactgtg ttatttaagt tcaaaaaatg aaaataaaat
    19441 gaccatgata caaaagaaat acctttttta ttttttttat ttaagctttg aaataaatat
    19501 aatattggtt attaagttga attattgatg tttaaatgta aaccaatcta tattaaaagt
    19561 attgcttcca gttgaactaa atgtgagatt cagagcatga ataccattaa cacgcttaac
    19621 tttacagcta aaaattttcc atgttttacc atctcctccc ccgtccacag ctatttctgc
    19681 aaaaggtaat gaccatgaat tatccagtat aaagctaatt tttccggaat ttaatccggg
    19741 tgcaatacgt attgagacgg aatcgactcc ttctccgaaa tcaatgtatt tatacatagc
    19801 acggtcttta tcttcaattt gccccagttg atcgctgtta gtcggattta aaggattatt
    19861 tgtgtcgaag gaataagatt ctacccggac atgacccgaa agcagacaag catgctctgc
    19921 ttggatttta gaaaaagcat caagcggttt tcctgcgccc tgcgaagtca tttctacttc
    19981 ggttatactt ccatccgtgt tgaaataaat cggttcaata caggctttcc gtgaattgac
    20041 acatccgttg gtagttctat gataaaaaac ataccattga cctttaaatt cgacaattga
    20101 tccatgattg ttccagttag caggatcaca accatcgttg tcgataataa ctcctccgta
    20161 tttaaatgga cccaaaggag attttgaagt ggaataacca atgcaggtag gcatattttt
    20221 ccgcccgaca tgggcataaa caaaatagta gattccgttt cttttgacca tatagccgcc
    20281 ttcgtggaaa aaatgctctt tttcagtaac cagattatca acaatggtag tggtatcaat
    20341 ctctgtcata ttaggtttta gtttggcaat ttttgcctga aattgacccc acatataata
    20401 cgcttgacca tcgtcatcca caaaaacagc aggatctatt tgattcttat ttttcagata
    20461 aatttcttta gcatttgtaa acggaccgat tggtgaaagg ctggtagcaa ctccttgaat
    20521 gccatccggc tggcaataat acaaataata agtgccattt ttatacatac aatcaggtgc
    20581 aaacaacaaa ttatcgttaa atggcacctg atcgtattgt cctttagacg caaaaacatt
    20641 cggtgtatat tgccagtgaa ttaaatcatc agtggacaaa acccagtaat caaaagaaca
    20701 ataataatta aaactttcgt cgcgtgaacc gtacacatat agccgtccat cattccagac
    20761 tcgtgcagaa gggtcggcaa tattcaaacc gggagggcaa ataggatttt gagctgtaag
    20821 catctcaaaa tatattagaa gcagtaataa taatgtgttt tttttcattc agaactgggt
    20881 tcaaattatc tatcgattca caaatgcttg acttataaaa taatattccg aatattacaa
    20941 taattatatt gcgatataat tacttgacaa tcaaaaaaaa taattgtcat tttttggtaa
    21001 agaccttgaa atttttcttt atagctgact aacaatcggt tataatgaaa acaactggat
    21061 taaatttaga agacactctt ttgagggtta tttataggat taaaaaactt gtcgctatag
    21121 taaccattgc attttgttgg gcatatctgg tcgaaatttt ttttacacga acatgaatag
    21181 ccaatcacaa tgctgaacaa tggtagaatg ggaaaaagtt atttcaaata tgaacttcat
    21241 tgcaatagtg ctttaaatgc gggttttaaa tcaaattctg atatttttaa atttttgtca
    21301 tgtacttaga aataaaaata aaatcttact ttgctgtatt tactaaatct ttttctacta
    21361 tcaaaaccca atctttacca aaaccggttg ttggagttgt aacacacatt ttaccggtat
    21421 taattgtttc tatgctttga attgtctcac atgtttgtgg attaaaccaa ctgattttca
    21481 gttttttagt tttaataaat gatgttttga ttgtaattgt tttaccaaca ggaatataaa
    21541 tcatggcata atcatttgat cgatttctga aagcacaaat atactcttct ttttctccct
    21601 gaccttccaa aataatggat tgatctggaa tcctgcttga tgatggtcgt gattcaataa
    21661 gttttttcaa atatccaacc tgaaatgcac cgggtctgtc caatgcttca gtccagtatc
    21721 tctccgcaaa attaatattt gggtcgcgtt cattcctgaa ctgccagata ctgtgattgc
    21781 cgtatgtaac accgcatgct ccggcaaaaa cagaacgata ggtctgcttg cgaacatcgt
    21841 aatctctgaa ataaccattt tcaggattcc aacttggcca cggattcaca ggatgatctt
    21901 cgtaatttgg ttccgaatct agagtgggct ttgttggctt catacaataa tcccgtttta
    21961 tccattccca cacaggaaca tcttttcccg aaccatgacc cgactgcatc atatttatat
    22021 ccagccattt ctcagaatga atatattgtg atgttgacat ttcacctccc cagatatgat
    22081 aagaaataag tgctgaatga ttggtagctt tttctattcc tttggccata tttctccaaa
    22141 caggtcgcca gtcggcacta tccttcatgg gtggtcggtc gccaccaagt atccatataa
    22201 tatttttata actcttatac ctttcgccta tccattttcc atatttatag gcgttttcgg
    22261 gattgaaaac caccggacct tcaccccaga gctgagtaac tttatctccc caagtgggta
    22321 gcaaacccat aaaaatacct ttttttgctg ctaattttac aaccgtatcg acgaactgaa
    22381 aatatttttc attaggtttt gtaggatcca tatctataag gggaacatcg ccgtatctgt
    22441 ttggaactct caatccatcc atttcaggaa gtacaactac ttgaacaaca ttaaatcctt
    22501 tttgttttct gttttccaaa tatgtttcaa tctcttccag ttttaaacga tgaaataatt
    22561 cccaagctgt atctccaagc cagaaaaatg ggtccccgtt tttataactc aaataatgat
    22621 ggtccgaagt tatttttaat tcagactgac ataaaatttt ttgtccaaac aatgaaatga
    22681 acaaaaaagt aattataaat aaaattctga ttttcttcat tttatagtaa aattatgttg
    22741 actattaaca attaaataat cgtatgcaaa agcaaaagct cctgccgacc aaccatacat
    22801 tagacttgca ttatattccc tgtcgtttat tgtgccaaat cgggtgtatt tctcataaat
    22861 tcctcctggc aatctgtaaa atttttttcc gcttttatct acataagttg atggatttgg
    22921 gttaaaaaaa ttggctgtta tcaggtctag atgtttcatg gcaatgcggc aggcatcttc
    22981 cttaaaacca taattatcaa gcgccatgat aaccaatgat tgcattggcg accagacgga
    23041 aatatgatcc cattggtaaa ttctcttttc atccgtgctt tcgcaggcta caattccgtt
    23101 ttcactctcg aacagatgca gattttcacg cattcggtct gcctgatatt tcgaggccag
    23161 acctgcaaag agcggtgaaa atgctgttgc agctgctact ttactgcttc tgttgtttac
    23221 aaaatcataa tccagaaata acccacgttc atcgttccaa cagtacttct gaataagcgc
    23281 ttttcttttt cgggcttttg ctaaccattg ctgaccatta cctattttaa gttcttgttc
    23341 aaaataagcc agatactttt cgtacaaata tagattggag ttcaaatcga cagctataaa
    23401 ctccgaacag cgattttcga aacggggcgt aaagtccatc cctgattcag cttcagcagc
    23461 aaattgcgaa gcaatattca attttttgga tttttcaata tcttttggaa agtcaaaacg
    23521 agaagtgagt tcctgatcat acagttcaag tagctctgtt tcggtggcat ggtgaaaata
    23581 gcgctgaaga ccaagttcag ttgtatttgc ctctattcgg ttcgccgatg tatctgtcca
    23641 gaatttgtat tctttcaaaa cggcagcata catcgttttt aaccattttt tatcttttgt
    23701 aaaatcgtaa aattctttta ccattatgcc aaaatagggt ggttgcgaac ggttgtcccc
    23761 ccaatccgta ttcgcattgg gtataaatcc acgcttgtcc agttccgctt tcaggttatt
    23821 tatccctcct ttagctagct ttagttcgtt atgtttcaat aatccgattt gtgtaaaata
    23881 caaatcccaa taaaataatg tccactcgtt aaatgcatac gcaaatgtgt cgggcacagc
    23941 attgttagat ggttttccgc tgtgattttt ccaaccggct ttcacactgt cgtttattgc
    24001 cgaccactta tcggcataca aagccgtata agcctcagga gtgaagcctg ttttactggt
    24061 ttttgagttt ttttgcgaac tacgacaacc tgaaaacagc aataaaaggg ctaacgctga
    24121 gaaaataaaa taatatcggc tcaccttttt catcaataaa aagacttatt tgagttttat
    24181 aactgaatag gattgtccat ctacctgtat ttcggttgtt cctgtatttt gtttccaaac
    24241 cgggagtttg ccgagcaata attttcgctt gcctttcagg tttccttcaa caattgctgt
    24301 tgccggttta ctatcgggat taagtattac caacaatttt tcgttaccaa atatccgcat
    24361 gaaaatgaaa ggatatttgt ttttttgaac atatacggga acaaattttg cataatttgt
    24421 caatgctggt tcagttttat gtaacaggac taatttccgc ataaaattta aaaatgaatc
    24481 cgggtcattt tcttgcgcta aaacattagg agcattcctt gaactgtcga ctggcaaata
    24541 gagtctttca ggagcactgg tcgaaaagcc aagattcgct gtggtatccc attgcattgg
    24601 tgtacgagct cctgctctgg taccgtagca tccttctttt cctgtaacga gcggcagttg
    24661 acgcatggta atttcatctc cgtaataaat aaacggaatg ttaggcatcg taaagttaaa
    24721 cacctgaatg atttccaaat cagccctgtt gcgccccctg ttatttattc tttccaaatc
    24781 gtgatttcct acagttaacg aaatatatcc tttgccattt gtttcagtgc tttgttcgtt
    24841 gtatcgccac ataaaatgac tgatacttcc gattcccgca gacctgaaat aactattttc
    24901 gtgcataaac aagtcctgat aaccttcctg ccaaagcata aaatcggcat gaaaaccggc
    24961 attgacagca gtagctgatt cactccactc tgacaccatg aatgcatcgg gatattcctg
    25021 atcaagcatt tttcgaatgt cctgccaaaa atacttaata cttccttcgg ggtcgttttt
    25081 taccagcgaa ccagccatat caaccctgaa tccatcgcag cccatttcaa gccaataacg
    25141 cataacattt ttcatttcgg tacgtagtgc aacgacatcg ggatgagtgg tgcttaattg
    25201 ccagggttgt ttcggatctg gctgaccgta accataattt aatgctggct gatgccagaa
    25261 aaagttaatc atgtaattgc catccctgtc gcaaaagccc tgaataaaat tcgctttata
    25321 cttgccgttt tcgtcatcga accaggttcc atttgtccat atataccagt tgctgtattt
    25381 tgttggcgtc gaactgcacg aggccttaaa ccacgggtga tcaatggagg tatgtcctgg
    25441 cacaaaatcc acaatgattc gaagtccttt ttgtttggca atccggaata aatctttggc
    25501 atcctgatcg gtaccgtagc gtgggtcaac atggtaataa tcggcgacat cgtaacctgc
    25561 atctctgaat ggcgatacat aaaacggatt aatccagata gcatctacac ccaagctttt
    25621 tacataatcc aactgactga ttacaccttt caaatcgcca ataccatcgc catttgtatc
    25681 tttaaacgat tgtggataaa tttgataaaa tgtagcattg tgtatccatt ctgggcctgc
    25741 aggtttttga caggttacag cacagtttaa acacggaaaa gctgagattg tatattcgcc
    25801 tttcccgcac tgtgcaaaac cagacaaaca aatattggca gaaacgataa ttaaagagag
    25861 tgatgacaaa aaagaaaatt tcatgatatt agatttttta aagtaacact actcaatatt
    25921 ataaagtgaa gacaaaaata acattatcca gtggataatt catatcattt tttgacagaa
    25981 tatgatactt tttcaattta atgataataa ggctatcttg acttttgaca aatagtgtat
    26041 aaagatacat taagctagcg cggtcgaaat aatataaaat gataattcgc aaaatatcct
    26101 aaattaaaag gctgacgtca ttttaaacac tgtcaaagaa cacataaatc caatggttga
    26161 atggatcacc gatgatgcta ctccttacaa caatttgaca aaaaatgtca aatcacatca
    26221 tgcacaggtt ataaagaaag acgacatgcc aaagatatta ccctggctgt acattgccat
    26281 tggcaatgtt aaacggttgc tcttggatgt acaccattaa cttacgaaag attatttact
    26341 aaagtttcgc acttcgcttt aatgatttga aagacaggaa ataaagaact gcgaaacttt
    26401 agttatttac aataaaggca aataaaaata ctgtattctt tttgcaatcg tttgcacgaa
    26461 aaaatctttt aatttttata gagaattcga tgttttagaa actaaacttt tacatttgca
    26521 tgtaagtcaa actgtttaaa tgctattatc atgaaaaaaa ttcttttcac tttttcatta
    26581 ctgctttgct atttattaag ctggggacaa atcattacta ccaatcccac ttttatcacc
    26641 aaagactacc cgggagaaat agaaattatt tatgacgcca cacaaggcac tgcaggtttg
    26701 aaagattaca ccggcgatgt atatgctcac acaggcgtaa tcacctcatc aagcacttca
    26761 ccgagcgatt ggaaacatgc acctgtttgg ggtgacaatt ctccaaaata taagttgact
    26821 tcgctgggca ataacaaatg gaagcttttg ataaccccca atatggcagg atattataat
    26881 ttaagtcccg gagaagttgt cagaaagttg gcttttgttt ttcgaaatag cactggtacc
    26941 ctacagggaa aagatatagg aggagcggat atctttgtaa ctgtttatga aagcaatttt
    27001 aatgtggctt ttactttccc aacctccaat cagagtgtcg ctatcggctc tagtatcgac
    27061 tttacagtta gttcttcgat gagtgccgaa cttaaattgt tgctcaacaa caccgttctg
    27121 gctacacaac aggcagccac ctcactaacc tattcctaca attttagtgt ggcaggcgat
    27181 tatactttta ttgcttctgc caaagtcgac aacaataccg tttacgatac cctgctggtt
    27241 tgcgttcccg aaccggttgt gaatcaaact cgtccggcag ggactaaact gggaatcaat
    27301 tacatcgaca acacaactgt aactttggtt tttcatgcac ccaataaaac caatgttttt
    27361 cttattggag aatttaatga ctgggcacaa ctgaatgcct atcaattgaa aaaagatggt
    27421 gaatactggt ggattacctt aaccggactg actccgggaa aaatttacgc ctatcaatat
    27481 ctggttgatg gaacgattcg ctgcagcgat ccttatactg aactcgtgct ggatccgtgg
    27541 aacgacaaat ggatcaatca gtattacacc atttatccaa atctgaaacc ttatcccgaa
    27601 ggtaaaactg aaggtctggt tgcaactttt caaactgcga aacctgccta taattgggaa
    27661 attaccaatt tttccatgcc ggcaaaagaa aatctggtga tttacgaaat gctgctgcgc
    27721 gatttcacta cagaaaaatc gcttcaagct gccatcggca aactcgatta tctgaaaaat
    27781 ctcggaatta cggctgtgga attaatgcct atacaggaat ttgatgggaa caacagttgg
    27841 ggatataatc ctaatcatta ttttgcgccg gataaagctt acggaacacc cgaaatgtac
    27901 aaaaaattta tcgacgaatg ccacaaaaga ggtatggctg taattctcga tatggttttc
    27961 aatcaggctt cgggaatttg tccgtttgca ctgctttatt gggacagcac aaaaaatcgc
    28021 cctgctgccg ataatccatg gatgaatccc gtggcacctc atcaattcag tgtattgaac
    28081 gatttcaatc atagttattc cggcaccaaa gaatatttca aaagagtgct gcagtattgg
    28141 attactgaat acaaagtaga tggctaccgg atggatttga ccaagggatt tacgcaaaac
    28201 tcgggaacgg aatcgacatg ggaccaaacc cgtatcaaca atctgaagga ttattattac
    28261 gcagtaaccg acgtgaaacc agatgcccta tttattctcg aacattttgt gggaggaagc
    28321 gaagaagatt atctggcagc tcagggtatg tatttgtggc gaaatttgaa taacgcatat
    28381 tcgcaggctg caatgggttt tcagagcaat agtgattttt ctggcatgaa tacttctccg
    28441 cgcagatggg tgggctatgc tgaaagtcat gatgaagaaa gaaatttcta taaagcaaaa
    28501 acttatggga atggcacagt taagactgat tctttataca gaatttcacg tgtacctttg
    28561 gtagtggcgt tcgcaacttt gatgcctggt ccaaaaatgt tgtggcaatt tgaagaaatg
    28621 ggttacgatt attccatcga ttcgttcgga ggccgaacca acccaaaacc tcacgcttgg
    28681 ggctggctga atttgccgca tcgaaaagcg gctgccgacg cctgtgcaaa aattatatct
    28741 atgcgtaaat tgtatcctaa tgcctttctg aacggaaatt attctttgca agtcgcctat
    28801 aacgactggt cgcaggggag aagaatagct ctctctcatg ctgacctgaa tatggtagtt
    28861 ttgggtaatt ttcaatctga tgcttcaata acagccaacc cgtcctttca acgcaccggc
    28921 acatggtaca atttgctgac aggcgaaacc ctgaatgtga gcaataccaa catgactata
    28981 aacatgcagc ccggccaact actgattttc actgaccgaa atgtaaattt tactaatgac
    29041 aacagagaaa tatcctataa tgtgagcgat tatgtttatc caactttgac aagcgggaaa
    29101 ttgttcattt cctctaccgc aaatatccaa agcattcgca tttataactt gcagggttcg
    29161 ttgcttaaac agcaaaacaa tgatataaaa gaaattgatc tttcaatgct gaaaaaagga
    29221 ttttacctgg ttgaactgga aacggcaaac ggcaaaagcg cacacaaaat tataaaggaa
    29281 taacgtagaa aaaaatgtgc gaaagtataa aaaaaaatta aataagcagg caataaaaat
    29341 ttacctgctt atttattaat tgctgaattc aagatacaag tacaacctgt tattatacat
    29401 atttcgttat aaaaatttat ctggttttaa gaaacccaca tgtattacat acacaaacac
    29461 gaatgaatat aatattcaga catgtgggtt ccatcggacc attaaaaaat aaattatatt
    29521 ctgatgaaat ttaatttttt tcttggtgta ctgaatgatg catttttatg tcgaacctgg
    29581 tttattgaat caatttaaca tttcagattt tttttgccgg ctgctaatca atttcgagtt
    29641 gcaaatcatt tctaagtttt gctaaagccg gattttcttc tgccattatt ttatatttct
    29701 cttcaggagt tcttcctttt tgtaccgacc tatcttcttg aatttgaata gacattttta
    29761 tgtttgaatt ttttaaagcg gtctttaaaa aattcaaaat agtcggcttc agccgtttta
    29821 attccttttc ttgtaactca ttggtaacta atattctaaa ttcagtattg gaaataagct
    29881 cgggacgatt tgactgcatg aaagcggtca ttcgtgcctc atctactaaa gttttagcaa
    29941 gttcagccca agctttttgc aagtcatcct gagtaaaaat ttcgttccga atatcaggct
    30001 gctccttttg tggtgcctca taggacgatg aaggagaaga aacaacattg tttttaatgg
    30061 aaatacttgt cggacgttcg gttttcacct gctttgcgtc aacatctgta ctcattggat
    30121 ttgacgttga atcttgaact ttagtcgatg aagggctatt tttagcgaga acaacattat
    30181 ttgttttcag ctcatcaacc acttttttca gaggttcatt tttctcttcc ccattgagtt
    30241 tatcctcctg tttgacaagt tgacaaagac gaatcagcat aatctctacc aacaatcgct
    30301 tgtttttgct gatattgtaa ttcaaatcgc aatcgttaca aattttcaag gctttaaata
    30361 tgaaatcttc aggacattgc tgagcttgca tttgataatg ttttcctata tcggcaccca
    30421 cttccagcaa ttgaatggtt tgaggatctt tactaaccaa caaatcccga aaatgagaac
    30481 tcagtccgga gacaaaatga tgtgcatcaa aaccgtgttg aattatatca ttgaaaataa
    30541 gaagagaagt gttcacatca tttttcataa aggcttctat caactgaaaa taataatcat
    30601 agtccaaaac attcagattg gtgagcacat cctgataaga aatattattt ccacacaagc
    30661 ttaccagctg atcgaaaata gagagcgcat cacgcattcc tccatcacat ttttgagcta
    30721 tgacatttag cccgttcaca tctaccgtta ttccttcttc atgagcaaca tattgcagat
    30781 gtcccacaat atctgatacg gaaattctat taaaatcata aatttggcag cgcgaaagaa
    30841 tggttggaat aattttatgc ttttcggtgg ttgccaaaat aaaaatggca tgagccggag
    30901 gttcttccag tgtttttaaa aaagcattga aggcactgga actcaacatg tgtacttcgt
    30961 cgataatata cacactgtat tttcctattt gcggtggaat acgaacctga tcaatcaatg
    31021 aacgaatgcc ttccacgcta ttgttggaag cagcatccaa ttcatgaatg ttataggaac
    31081 gttgctcatt aaaagctttg catgactcgc actcgttgca agcctcaaag tcttcggtaa
    31141 ggtgctcaca attgatggtt tttgcaaaaa tgcgagcaca agttgttttt cctactcctc
    31201 gtgtaccaca aaaaagataa gcgtgagcca aatgattact ttttatggca tttttcaaag
    31261 tattggtcag agcagtctgt ccaactaccg atttgaaagt tgtaggcctg tatttgcgcg
    31321 ccgaaacaac gtaatttttc atactgttgt aaaaataaac acaatgttat tgattttaag
    31381 acaattataa aaacattttg gaataaatat tttgttgcaa ttccagttgt ttttcttctt
    31441 tttcatgcaa acttacataa aaaaaatgat ttttacaggc tgctcaccaa aaataaagga
    31501 aggattgttc aatggaaaat tatcgtaccg ttggaaataa tggttaatat gtatatccga
    31561 ataaaattcc taacagtaac atagcttaca tttataagtc tcatgaatga cgttgaatac
    31621 attatttcca tgcagttgtt tctttgctta caaaggatat atgaaaccca catgtattgc
    31681 atacacaaac acgaatgaat ataatattca gacatgtggg ttccatcaga cctttaaaaa
    31741 ataaattata ttctgatgaa aattcattga ctttttttat cactccgcat tagaattacc
    31801 tcaaaactat tttttctcca tttatgaatt ttggttatgt tttttgtaga aatgaagaaa
    31861 aaaaaagcag gcgaaaagcc tgcctgaatg attatttata tagaaacact aaattcattc
    31921 ctttgtataa ccccatttca gatatacggc gccccatgta aagcccgcac caaaggctgt
    31981 taaaattata ttgtcgcctt ttttgaattt tttctccgat tcccacatac ataaaggaat
    32041 tgtagcagca gaagtgttcc cgtatcgact gatgttgatg attacttttt catactccag
    32101 accaatacgt tgagccacag catctataat gcgtaaattg gcttggtggg gaattagcca
    32161 tgcaatatcg tcttttgtca gatgatttct ttcaagaatt ttcactgtag catccgacat
    32221 atcggaaacc gcatgtttga aaacaaactg accttcctga tagatgtagt gcatgcgctt
    32281 gtcaacagtt tcgtgagaag caggataaac tgagcctcca ccctttaaat taagatgaga
    32341 gataccaact ccatcggtaa ataagataga atcaatgatt ccgacgtttt cggttgtagg
    32401 ttcaaccaaa acagctcctg cagcatctcc aaaaagagga gcagtagtgc ggtcttcata
    32461 attggttatg gatgacattt tttcggctcc aataaccagt atttttttaa atctgcccga
    32521 ttctattaaa cctgtggctg tggtcaaacc cacgataaac cccgagcagg cagcttgaat
    32581 atcaaatgcg accgcatttt taattccaac attttgagtt actattgctg cacatgccgg
    32641 aaatatgtaa tcgggtgtag tagtagcgca tagtaccata tcaatttcat ctggattcgt
    32701 acctgatttg gacaacaaat cttcgacagc tttggaggcc atgaaggatg ttccaagttc
    32761 cttatccttc agaatgcgac gttccttgat gccaacacga gtggtgatcc attcgtcatt
    32821 ggtatccatc atggtgctta gttcttcatt ggtaagaaca taatcgggaa catatcctcc
    32881 cacaccggtg atgactgcat taattttaga catattgtaa atatattaat aaaaaatcaa
    32941 gcccaaagct gaatttcttc ctcagggccc aattgtttta tacattacga ttttaaaaat
    33001 tttttcaaaa ttcaatgctt tatttaagca ttagccagtt tttcgatagc cagttttcct
    33061 ctataataac cacattcgcc acaaacggtg tggtaaatat acgttgcgcc acagttggta
    33121 caaattgcca gcgttgggag tttggcctta tcgtgggttc ttctttttgc ggttctctga
    33181 ttcgagtgtt ttctcttagg atgtgccatt ttattttatt ttttttattt aattgtcaat
    33241 attctgtaag ttgtcaaatt ctaaatcaat gaactgattg tttgaaaaat aatcgtcttc
    33301 gttggtatct tcaagttcca aagaagtttc atcctcgtat tcctttgtgg atgaaatgtg
    33361 cttttttagt ttggttatca ttgctttgtt acattctccc ggtgcatgta catgtttcaa
    33421 tggaatgttc agtacgataa attcgtaaag aaaccaagcc acatttattg ctccatcagc
    33481 ttcaggtacg ataactatat cgttttcctc cgaaaatttg ttgccaaact ttacttctaa
    33541 ggactccttg taatgaatga gttgttgcat atcttccaag caacgggagc aaggcaaagt
    33601 tataaaacca ttcagttcga aatcgagttt ataaaccgta gttttttttt gtacggttat
    33661 ttttgcatgt acgttgcctt gttgtacttc agggctatcg attttctgaa aataagcatc
    33721 gtccaaatca aattctacaa tgcgtttttc attggtaaga tctttcaata caatgttgta
    33781 aagctcaaat ttcgacatga cacaataact ttaaaaagcg gtacaaaggt acaaaattct
    33841 atattatttt gaaatttttt ccgtttattt tatcaatttt ataagcaaac actttatata
    33901 aacatggttt gttgcttttt ttcaagcttt catcaaaatc cgaaaagtgg ttcctttgtc
    33961 gatttcggac tgcttgacaa aaatttttcc cttgtggtat tcctcaataa ttcttttggc
    34021 cagcgaaagt ccgagccccc aaccccttgg tttggttgta tatccagtgg agaaaacggc
    34081 tttaaattta tttttttcta ttccttttcc tgtgtctttg acgtcaacaa tgtattcgtt
    34141 agattttttt tggacgaaaa tgtcaatttt tccatttcca ttcattgcat caatggcatt
    34201 tttgcagaga ttttcaatta cccattcgaa aagcggaacg ttcatccgaa tcaggtcatt
    34261 atcagaagtt tgaaaatggc atgtgattat tactttctgc gatgaacgat ggcttatgta
    34321 ttgtactgca tttgtgatcg tttcattcag ttttactagt tgaagatcag gagctgagcc
    34381 aatttttgaa aatcgttctg caataatttt cagtctgttg acgtcttttt ccatttctgt
    34441 cagcaatttg tcatcattgc gtattcggag tagatccagc caagcaagca gagaagagat
    34501 tggggttccc aattggtgag ctgtctcttt cgaaagtcct acccatactt gattttgttc
    34561 ggcctttttt gttcccgaaa aagccagata ggctataagc aaaaaaataa aaattacacc
    34621 taactgaatg tacggaaaat atcttaattg ttttagtaat aaagaatcgt cataataaat
    34681 atgttgtaac gtgttgtcaa ttcttacttc aatatgtgaa ttttttttct ttagccgttc
    34741 aatttctctc cgataatagt cattggtttt tgaagcaggt tcatttacgt taagagaaga
    34801 aattaagtta tcattttcat cggtcataat aacgggaatg gttgtgtttc ccgaaattat
    34861 ttccgagaga aaatttatat cggtattttc tccggctaag atcacctgtc gcatggcacc
    34921 tgcccaaatt tccatttttt tgtgctcttc tgttgcaagt tgacgagtga gtctgttggt
    34981 gaaaaatagg gatgccgtta ctatcaatgc agcgcttcca ataaatacga gccttaggcg
    35041 ctgggataac cgatatattt tattaaaaga aaacatgaat aataattatc ttgaaaaaat
    35101 atggttcaaa tttacatgat atttttcgat tctcataaaa agtggaaaaa ttgattggga
    35161 ttgaaaattt ggttgatgtg aaaagctttc tttaatgaag taaattttga atttcaaaat
    35221 tcttttcatg ggtggatctt ttttaaagat ttcgttggat ttctccacta tgcctactat
    35281 tgttctcaaa tactggttct ttcctcttct tgcataattt caggatttct tcgtaaaaaa
    35341 ctatccttat gttttcagaa aatcgacata cttaattaag gaaaaatttc tttaattcaa
    35401 aaggaatccc taactttgca aacaaaaaag taaggtttaa gaataaaact ttcaagcaaa
    35461 attccactca aatacaagga aatatggctt ttttaaacaa tacattgata gtaagaagag
    35521 atttaatgac aaaattggta agcttgttca atgaaggtgt acttttaagt gaaatcgatc
    35581 gggttcctct taaaatgtcg cctaagaaag cacaagcacg aggacgatgt tgcattcaca
    35641 aggaaagagc ggtgttaaag tacaagataa tgccaattct tggatacagc atagaagagg
    35701 aagaagatga actcactccg ctttctgaat atgcaaggaa agcacttctg agaacagaga
    35761 aaaataaatc gatatttact gttgtggatg aagcctgcac agcatgtgta aagaccaatt
    35821 acgtagttac caatttgtgt aaaggttgcg tagcacgagc ctgttacatg aattgcccaa
    35881 gagaagccgt tttttttaaa gaaaacggtc aggctggaat tgatcataat aaatgtataa
    35941 attgcggaat ttgtaaagaa gcatgtcctt atcactccat agttttcgtt cctgttccgt
    36001 gcgaagaatc atgtcccgtg aaggccatac aaaaagatga aaacgggatt gaacatattg
    36061 atgaatcaaa atgtattttt tgcggaaaat gcatcaatgc ctgcccattt ggctcaattt
    36121 ttgaaatttc tcaaatattt gatgttttat cggcaatcca aagaaatgaa aaaattacag
    36181 ctattgtggc tccttccgtt cagtctcaat ttgaacattc tttttcggat atagcaaccg
    36241 ctataaaaaa tataggattt tctgaaatca tagaagtagc gctgggggca atggaaacag
    36301 ctttccatga aggagaagaa ttgcagcaaa aattggagca gggtcagcaa tttatgacta
    36361 cttcctgctg tccctcttac gttgaactta ccaagaaaca tctgattgaa atgaagccat
    36421 tcgtttctca aaccaattca cccatgtatt atgcctctaa aatagcgaaa aataaatatc
    36481 ccgattcaaa agtatgtttc atcggtccct gtgttggaaa aagaaaagag gcagccgatg
    36541 atcaaacgat tgattacgta ttgacttttg aagaattaga tgctatttta aagggaatga
    36601 aaatagaaat cataccaact gaaaataagg atgatgaaga aataaaagct ggtagaggat
    36661 ttgctcaatc cggcggagta attgctgcgg ttatggagtc ttacccaaat gtaggaataa
    36721 agccgttgat agttactaat attgacaaga aaaatattgc tctgctcaaa gcatacgcca
    36781 aagggaaagc accgggaaat tttatagaag taatggcttg cgaaggtggc tgcatttctg
    36841 gtccttgcac gataaatacg tataaggatg cacaaaaaat atttaaaaag gttgttgagg
    36901 aaaattgaaa gaaattttaa ttttgactaa ttttgccgaa atcatttgaa ttttttatta
    36961 aaaatttagc tcaatttcca ttcaaaagaa ttaaaattaa cgtcaagata ttacctgacg
    37021 ataaatcgtt taaaatggcc gaaaaaactt tactggaaaa actggaagga ctacaaaata
    37081 aatttgacga aatttcgaca ttaatcaccg atccgaatgt tatttccgat caaaaacgtt
    37141 ttgccaagct caacaaggaa tatcacgatt tacgcgaaat tttaaacgta aaaaacgaat
    37201 atgaattggc attatcgcac ttgactgaag caaaacaaat ggtggaaacc gaaagtgata
    37261 cagaaatgcg tgaattggca aggtcggaaa tcgaagaact ggagcaaaaa attccggaat
    37321 tggaaaacaa gctgaaattt ttattaattc cggcggatcc ggaagacagg aaaaatgcca
    37381 ttgtcgaaat aagaggcgga actggtggag atgaagcagc aatttttgca ggcgaccttt
    37441 ttcacatgta tgccaaattt tgcgaaagca aaggttggaa aattgaagtt accgattata
    37501 atgaaggtac ggcaggaggt tacaaggaaa taatttttac agtatccggg gaaaatgtct
    37561 atggcacatt gaaatacgaa tccggtgtac atcgcgttca aagagttcct caaacggaaa
    37621 cacagggacg aattcacact tcggcggcga cggtggccgt gtttccggaa gctgaagagt
    37681 ttgatgtgga tttaaaagaa tccgacattc gcgtagatat ttattgttct tcaggacccg
    37741 gcggacaatc ggtcaatacc acttattctg ccgttcggtt gatacatatt cctacaggaa
    37801 tcaccgtgca atgtcaggat gaaaagtcgc aacacaaaaa caaggcaaaa gccatgattg
    37861 aacttcgttc aagaatttat gccattgaac atcaaaaata tcttgacgaa atcggttcaa
    37921 gacgtaaaac aatggtatct accggcgatc gatcggccaa aattcgtacc tataattatc
    37981 cgcaaggaag agttaccgat catcgcatca attacactat ttacaacctg cccgcattta
    38041 tgaatgggga aattcagaca atgattgatc agcttatgat tgcagaaaat gctgaaagat
    38101 taaaagatgc agaattataa catatgggat attgataaat tccaacaaaa tgaaccgaaa
    38161 agaacttttt gaaaatatta aaaggaaaaa atcatttcta tgtatcggat tggataccga
    38221 tataaataaa attcctattt atttaaaaga aaaatcatca aatccaatat ttgattttaa
    38281 taaagttata gtcgatgcga cggcagattt atgcgttgcg tacaaaccca atctggcttt
    38341 ttacgaaagt ctcgggatag aaggtttgaa agctctgaat gaaacagttg cttacattag
    38401 gaatcatcat cccgatcaat ttttgattgc cgatgcaaaa cgtggcgata tcggcaacac
    38461 ttcggccatg tatgcgcaag ctttttttga aaaaatgaat ttcgacgcca taaccgtttc
    38521 tccgtatatg ggtgaagatt ccattaaacc ttttctttcc tataatagca aatggaccat
    38581 tattcttgca ctaacttcca ataaaggagc tttcgacttt caactgataa aggaagaaaa
    38641 taccagagaa agattgtttg aaaaagtttt gaaaacatct acaaattggg gaacggaaga
    38701 aaatataatg tatgtcgttg gcgcaacaaa aacagaatat ctaaaatcgg taagagaaat
    38761 cgttcctgaa cattttcttt taattcccgg tattggggcg cagggaggaa gtcttgaaga
    38821 aacggcaaaa tttggattga ccagtcagtg tggtttgttg gtaaattcat cgcgtcaaat
    38881 tatctatgcc tcttcggatg aaaactttgc cgaagcagct cgctttgaag cattgcaact
    38941 tcaacaggca atggaaaata ttttgttaaa aagtggcatt atttgagctg ccatcctttt
    39001 tttaatctaa aaagggcgac ttcaaacttg gagtcgccct ttaattttaa atcaactatt
    39061 tatttttact ttgaaatttc aaattctacg cgacgatttt tctgtctacc tgcaggagta
    39121 tcatttgaag caataggttt atcttcaccg taaccggcaa cagtaatggc agaagcagga
    39181 ataccctttt taacaagata atcctttaca gcttgagcac gagcaatcga caactgttga
    39241 ttaaccgatt ttggtccaat attgtcagta tgacccgaaa cgataatttt tgtccaagca
    39301 gtagaattac tattcaaaat tgcataaatc tgatccagca aatcataagc ttgtttggtt
    39361 aatttagaag aaccaaattc aaattcgata ttttggaaag ttgcattaac agtaccggtt
    39421 gatttctgcg ataaagcctt taaatcgctt tcaagttgtt gcaatttgtt ctttatcatg
    39481 gcattgtcat tttccaaagc tttcaaacga ttttccgtat tggcgtcaac ttttccatct
    39541 tgaacagttt tttcgataac aaccggtgcc ggagccggat aatattcttc caaagacatg
    39601 tcgcgaacat gagattttgt agctccgcca aatttataac gcagtccaat tgtaccgatc
    39661 aaagcatcac tacctaaaga attgttactc aacccgccta aattttctct catataataa
    39721 cggtactgac cttccactcc caacgacaat actttgctga gacgatattc ggcatttaga
    39781 gcagcaaatg caagtggaga tttcaaatta tcaccatcag taactcctgc aacacctgaa
    39841 taagagtaga atcccaaccc tgcgcccaga tttccataaa tatttacttt accccaaaag
    39901 ccagcacgtt ttggagccag taaattactt aagtttgtag aaccatacaa ggtaaaatca
    39961 agcgtatgac ccgcacccgt atttctgcca taagtcagat aactgaaatc ggcacccaat
    40021 ccaaaaagtg gattaaaagt gtattctaca taaatttgtg gagcagtcca tgctgcattg
    40081 ttggcataac gcgcccaaga attttcaaaa aaaagataat ctgtaacagt actggaaggc
    40141 gtaacccgat agtaatccac tccagcctta ataccggccg accaatgagc aatatcggct
    40201 ttcgctttag tttctgccgt gctttgagca gatgcgtaag ttacagcaac tgccaataac
    40261 ataacgctca ataagatttt tttcataatt ctactttttt agttaattga ttttccgaat
    40321 attattttga ttaaaaaaga taaataggat ttattgaaaa cacacctcaa taaaacttaa
    40381 aaatttattt cctgcctcta atttataatt caagcatcgg cattcaccgc aaaattaata
    40441 aaattacttt aatgcaaata atttttaata gaaatatcgg caaatatatt tatttttttt
    40501 caaaatcaaa ataattttcg aacttttttt ctaaaaaatt atttataata atcaaaaaaa
    40561 tcacgcaaca ataaaaattt aaatcgaaga aagtttttaa agcaaaattt ttgattctaa
    40621 agaaagattt ttttcagtaa ctttttacaa taattatgcc ataaaatctg tgttcttaag
    40681 gtgcaagtct tgaaatttcc cattgttgat cgttttttgt gtagaacaat cgatcgtgca
    40741 ttctgtcagg ccttccttgc caaaattcaa aagttttggg gcgaaccaga aacacgcccc
    40801 aaaaaggtgg tcgtggcacc tcagtttcaa acttatgctt ataatactcg aattgtttgt
    40861 caagaaaatc tcttgacgga atttttcgac tttgaggtga tgcccatgca ccaatttgac
    40921 tgaataaagg acgcatttta aaatattcgt cggaagcttg ctctgcaatt ttttcggcat
    40981 tgcctgttat gcgtacctga cgctccaaaa ccggccagaa aaacaacaga gaaactcttt
    41041 gattatgaag aatttgcaat cccttattcc ctttatagtt tgtaaagaaa acaaatcctt
    41101 cgtcagtcaa ttctttcaat aaaaccattc tgccatgagg ttgccattct gcatcaacag
    41161 tagatagaac cattgcattg ggttcagatt cctcagtatt tatggcttcg ctcagccaca
    41221 acttaaactg ttgaaaagga ttttttaaaa tattcgattc attcagagta gatcggtcgt
    41281 actgtttgcg taaatcttta agcataaaag caatttatta cacctttata ctcaaataac
    41341 acttttaaga aatatttgtt ttaaatcgaa agagaaaatt ttaaaacaat acctcaacaa
    41401 aattatagga ttaatcgtgc aaataaccaa agtctttgag ctttttctcc ttattcctcc
    41461 aatctttttc gacttttaca taaaggctaa gaaaaacttt ttgtccgaga aattcttcta
    41521 tatcttttct agcctgtaca ccaacctttt tcaacgattt tccaccctta ccaataatga
    41581 ttcctttttg ggaatctctt tcaacataaa tcacagcacc tattctgaca agattttcct
    41641 cttcatgaaa ttcttctatc tccacttcaa cggcataagg cacctccttc tgataattca
    41701 acaacacttt ttcgcgaatg atttcactaa taaaaaaacg tacaggtttg tcggttaact
    41761 ggtctttgtc aaaaaaagga ggagattcgg gcaatagttc tttgattcgc ttcataaggt
    41821 aatccacgtt gaacttttcg agtgcagaaa tgggcagtat ttcacctcga ggaaaaatgc
    41881 catgccattt ttcaaccaac aattccaatt tttcctgatt aataagatct atcttgttga
    41941 ttaaaaccaa taaaggaaca ttatcagaat taattttttg aacaaaatcc atatcttttg
    42001 gttctgcatc atacacatca gtaacataca tcagaatatc ggcatcggtg agagcagaac
    42061 gtgaaaattg caacatggat tcctgcaaag catattttgg tttaagaatg cccggcgtgt
    42121 ccgaaaaaat catttgataa ttttcctcgt tgactattcc caaaattcga tgacgggttg
    42181 tttgcgattt tgaggtgatg atagaaagtt tctccccaac aagggcattc atcagcgtag
    42241 atttacctac gttgggatta ccgactaaat ttataaaacc cgatttatgc ataatttaat
    42301 accaacttta tgatgatttc gattcatcca caaaattaaa aaattattga ttaaagacga
    42361 ttatcagtca aatttttcta cgctccgaaa aacacaaaag caaatatcga aaaaatttcc
    42421 cgagttgaaa taaaaacata aaaatcattt taaaaaaaat tttacctcta actatttgta
    42481 aatcgaaaga aaaagagtat ttttgcagtc cgtttattat ccaaaataaa aagttcattg
    42541 gtataattta ttgtctttat tttgtattga ttagccattc caaaaataag ataaaaggta
    42601 cctaagtaaa aagaatttat taatttattt aaaccattag taaaagtgga tactttaagt
    42661 tataaaacca tttctgccaa taaaaacacc gtgcagaaag aatgggtttt gatagatgca
    42721 agtgatgaga tattgggtcg catgggaaca aaagtggcca agcttctgcg tggaaaatat
    42781 aaaccaaatt tcacaccaca tgtagattgt ggtgataatg tgattattat caacgccagc
    42841 aaaataaaat tgacaggcaa caaatggaca gatcgtattt acttacgaca taccggttat
    42901 cccggagggc aaagagaaac cacccctgcc cagatgttgg caaaagatcc tgaaagactt
    42961 atccgtaagg taataaaagg tatgttgccc aaaaacagat tgggagcaaa acttttaaaa
    43021 aatctttacg tattccccaa tgcagaacac aatatgcagg ctcaacagcc aaagcaaatc
    43081 gatttaaact cacttaaata atcagtatgg aaatagtaaa tgccttagga agaagaaaag
    43141 cagctgttgc tcgtgttttt gttaacgaag gaacaggaaa aatcataatc aacaaacgag
    43201 aattgtccgt ttatttccca tcgcttcttt tgcaatacat tgtaaaacaa cctttggaca
    43261 agttgaatgt gactgagaaa tatgatatca aggttaattt gaagggaggc ggattcaaag
    43321 gacaagctga agctttacgt ttagccattg ctcgtgcttt agtaaaaata aatcccgaag
    43381 ataaacctgc tttgaaatcg gaaggtttct taactcgcga cccgagaatt gtagaaagaa
    43441 aaaaaccggg tcgtcccaaa gcacgcaaaa gatttcagtt ctcaaaacga taatatcaat
    43501 ctgttgtttc tctttttatt atcaaatgat atttgtaaca aaaagtcaca caaacagcga
    43561 attgaatttg gtttagtatc taaattgtgg agactctttt aaaaataaag actactcaac
    43621 aattgaattt cacagaaagt aaacaaatta aaaaaagtaa aatgtcaaga acaaattttg
    43681 aagaattatt agaagctggt tgtcattttg gccatttaaa aagaaaatgg aatcctgcaa
    43741 tggctccata cattttcatg gaacgaaatg gtattcacat tatcgattta cacaaaacag
    43801 ctgcaaaaat tgatgaagct gctcaagcaa tgaagcaaat agcaaaatcg ggaagaaaaa
    43861 tactatttgt agctaccaaa aagcaagcaa aggatttagt agccgaaaaa gcaaaagctg
    43921 taggaatgcc ttatataacg gaacgctggc cgggtggcat gctcaccaac tttcctacca
    43981 tacgtaaagc tatcaagaaa atgtcgacca tagacaaaat gatgactgat ggtacatttg
    44041 acaatatttc gaaacgagaa aaactgcaaa ttacacgcga aagagaaaaa ttggaaaaaa
    44101 atcttggttc tattaccgat ttaacacgtt tgcctgcagc catttttgtg gttgatgtaa
    44161 tgaaagaaca tattgcggtt aaggaagccg aaaaacttgg aattcctatt tttgggattg
    44221 tggatactaa ttctgacccc agtaatatcg attttgttat tccggccaat gatgatgcaa
    44281 ccaaatccat tgaagttatt ttgaatgctc taattgatgc tattcaggaa ggaattgaag
    44341 agcgaaaact cgaaaaagtt gagactgagg ctgcagaagc acctgtagtg aaagaaagaa
    44401 agtccagggt aaataaacga cccagaacac aacgtgaaga cgatgaggct cttaaagcca
    44461 acgtggttaa caaatttata aaagaagaag aaaattaaat aatgagaaca tttgactgag
    44521 aaaaatttta atttaataaa ttcgttcaaa agtattatta tttacagtct tgatctataa
    44581 taacgagtta cggttataat tcatttgtag ctggtagctc gttttagtta caacaacatt
    44641 tataaacaat taaaacatct tttttttcat gtatgcatga aattgaagat atttttcaga
    44701 atttaaatca aaaaattatg gcagtaacat tgcaagaaat tacaaaactt cgtactatga
    44761 ctggtgcagg tcttatggat tgtaagaatg cgctgataga agcaaacaac gatctggaaa
    44821 aagcaataga aattatcaga aaaaaaggtc aggcaatcgc agccaagcgc agcggaagag
    44881 aagcatcaga aggttgtgtg cttgccgcag ccaaagatgg ctttgcagca gtacttgccc
    44941 tgaaatgcga aaccgacttc gttgccaaaa atgctgactt tatggcttta actcaatcca
    45001 ttctggatgt ggcaatggat aaaaaaccgg caacacttga tgaattaaaa gcttttacta
    45061 ttaacggtcg taccattgcc gacttggttg tggacgaatc ggctattacc ggcgaaaaag
    45121 ttgaattgga tgactataat tttgtaaagg gcggtacaac agccgcttat attcatcaag
    45181 gaaataagct ggcaactatt gttgccttca aagaagaaaa tgtagatcat caggttgcac
    45241 gcgacattgc catgcaaata gcagcaatga atccaatagc tatcgaccgt gcttcggttc
    45301 cggctcatat cattgaaaca gaactcgaaa tagggcgcga aaaagctcgt gaagaaggca
    45361 aacctgaaaa tattatcgac aaaattgctg aaggcagact caacaaattt tttcaggaat
    45421 cggttttatt ggaacaaaca tccgttaaag acagtaaaat tacaatcgga gaatatctga
    45481 aagctcatca tgcaactata atcgatttta agagagtcag tctgaatctt gattaaaaat
    45541 tttttatata tgaaaagaca aaaacgggag aaaatgattt ttcttccgtt tttttcaaaa
    45601 tcaaaaaagt taaaatggta gaatttcgct gctctttttc aggattgcac caattgaaga
    45661 atttcctcat gcagcaattt ggtacgttgc agtatcagcg aattttttac ttgcaattta
    45721 tggtaacgaa atttaacttt aagttttctg aattttttat gccatagaaa agcacatttt
    45781 cccagataat attgcaaggg taaaaataca gccactgccc aaagtgaatc cttcaccttt
    45841 ttgtagaaca ataccgattg caaaatgtaa aacagaggaa aggttaccat gcctaatcca
    45901 aaacgcaagg agccttttcc accgggaaaa ttgatatgaa aagccttcat gataatgtcg
    45961 ggagaaaaaa atggcaatgc attcaaaacg agaccaatga gaaaaaatgg gaaagtgatt
    46021 acgagcaata aactttcgag taaaagagaa gaaactttca aatttttatc cagcaaccag
    46081 gatggtacgt tcaacttgcg caagttttcc ctgtattcct tgcaaattac attcaatttg
    46141 tcgatagtct ccggtttgtt cttttccatt tcaagcaatt ggcgagctgt tttttggcgg
    46201 gcaagaaaac gatatttttc tgtattggga agtttcatct tttcaaccat tgctgcactt
    46261 gctgctatgg tggccgtttc aaagcattcg tagttctttt cggtggcaat atccaaagta
    46321 acttcactca aaccgtcgcg cagcctgtcg cgaagttgat taatggcagc aggctggttt
    46381 tcttcatatt cattcatgta ttcgcaaact tctatcggtt tgccaacatt tattataatg
    46441 tgttcaccgt acttgtcgat atttccccaa tccaaaccaa cgggaacgat tttaactccg
    46501 ggttgattgc catatttttg ttgtgccatg aaggcaattc ggaaaattcc ttttgccagc
    46561 ggacgcaatt ttcgtttact tccctgattt ccctccggca tgatgccaat ggcctgatta
    46621 tgattcagaa catcaacgca aacctcaaaa acttcattat ttttgtcgac gttttctaaa
    46681 ccttcgcgca tgcggaatgc cggtaaaatt ttacaaaaat gcagaaatct ggcaatcagt
    46741 ttgttattga aataatcgga acgtgccaaa aagactaccg gcattttatg aggaactacc
    46801 gaaataacgg ccagcgcatc cataagtgcg tttaaatgat tgggcgcaaa aatcaccggc
    46861 ccggtttcag ggatattttc ttttcccata acaatgtatc ggctgaagaa ttttctgaaa
    46921 gcaaacatta cataactttt gaataagcta taagataatg taaaatcata aattttcgac
    46981 attgtatatc ttttatattg gttgttaatt aattgcagct aaaatttgct caaaaaattt
    47041 ttaaaattgc cgtgtcaatc gaattctggg aaagaggttt aaaattagtc attcatgttt
    47101 catataaaaa attattcgct gtaaacctca ttcagaaaaa atgaattaga gaacaaaatc
    47161 taaattttcc tacaaaaatt gttcctttct tcaaaaagtt tttttgctca cctcaacaat
    47221 cagtttttga attaatttct caattctttt cagaaagatt gcatatcata caaccttttg
    47281 taatagccgt ttaaagcaat caactcatca tgttttccgc gttccacaat ttttccttcg
    47341 gacatcacac aaatttcgtc agcccgttta atggtggaca agcgatgtgc aatgaccagt
    47401 gtagttcggt tcatcattag tttttctatc gcttcctgca ccattttttc cgattcggtg
    47461 tcgagagcag aagttgcctc gtccaaaatc agaattggag ggttttttag aatagcgcgt
    47521 gcaatgctga tgcgttggcg ctgacccccc gaaagcttgc ttccgcggtc gccaatagtt
    47581 gtctggtaac cattttcgga agccataatg aaatcgtgag cgttggctat ttttgcagct
    47641 tccatcactt gttccaatgt gacattttca acgccaaaag caatgttgtt gaaaaaagta
    47701 tcgttaaaca ggatggcttc ctgatttaca tttcccatca atgagcggat gtcttttgtg
    47761 ttcagatctt tgatattgat gccgtcgatc agaatttctc cttccgttac atcgtaaaaa
    47821 cgaggcaaca gatcgaccaa agtagatttt cctgcaccgg actggccaac caaagcaacg
    47881 gtttttcctt tcggtatggt cagattgatg tcttgcaaca cccaatcgct ttgatagcga
    47941 aagttaacgt ttttgaattc gatgcgatcg ttaaaagaat taattttttt cgattcggta
    48001 ggctcgggaa gtgtattttc ggctttcaga attttatcga cgcgttcaag cgaagccaaa
    48061 cctttttgaa cgctgtaacc tgctttagac aaatcctttg ccgggtttat gatactgtaa
    48121 aaaattacca aatagtaaat aaattcggca gcattgatgg aactgtggtt ttccaaaatc
    48181 agtacacctc caaaccaaag taaaatggca ataactacgg ttccgagcag ctcactgagt
    48241 ggatgtgcca acgtgtagcg ccggttgatt cgattgagtg tacggcgaag tttttcgttt
    48301 aaagctgcaa atcttttttc cagttttttt tcggcattga aagctttaat cactctcagt
    48361 ccgcttaaag tttcttctat ttgcgaaagc aattcgccgg tttgctgctg acccaatgta
    48421 gagcgctgtt tcaacgattt gccgatcatc ccgatcaacc aaccgctgag aggcaaaagt
    48481 atcaatacaa aaaatgtcag tttccagctc atggcaaaca ttacggtcaa gtatatcaga
    48541 ataatgatgg gatttttgaa aatcatttcc aaagaactga tgatggaggt ttcaatttcg
    48601 gtaacatcgc tggtcatgcg cgacattatg tcgcccttgc gttcttcggt aaaaaatccg
    48661 atgggaaggc tgagaatttt tttataaagt tgatttctca aatcgcgcaa cacacctgta
    48721 cgtatgggaa taatgtaata cgacgacaga taggaagtgc cggttttgaa aactgtcatg
    48781 acaatcagaa aaaggcccag ataaagcaaa gtcatcgaag gaccggcagt agtcatcatt
    48841 tgttcaagat agtaaaaaat attgtttttc agcgctgaaa taatttgatt ccatccatcg
    48901 ccccaagtcc atgcctgata actcagattc gactgctgca aaccaaagag aatctgcaaa
    48961 acaggaataa tggcagcaaa agagaaaaga ctgaaaactg tcgacaataa attgaacaac
    49021 agattgagga aaacatattt tttgtaagga ggaatgaaac gcttgagcag ttcaaaaaat
    49081 ttaaacattg aatttaatat atctttttgt aaaacaatac aataatcaga ttcccgtata
    49141 agagaaaggg gaaatttttc ttaattcgtt tttcacttct tcactgactt gcaacgaatc
    49201 gataaagtca tggatagttt gctctgtaat gacttcgttg gtgcgggtca aagcttttaa
    49261 ggtttcgtag ggagcgggat aaccttcacg tcgaagaata gtttgaattg cttcggctac
    49321 aactgcccag ttgttttcaa ggtcgcgtcg aatggccgat tcgttcaaaa ccagtttatt
    49381 catccctttc atcaaacttt gcgtggctat cagaatataa gaaaaaggta aaccaatatt
    49441 tctaatcaca gtgctgtcgg tcaaatctct ctgcaaacgg gaaataggaa gtttggccga
    49501 taggaaatcg agtacggcat tggccatgcc caaatttcct tctgcatttt cgaaatcaat
    49561 aggattcact ttgtgtggca tggcagacga acccacttct cctggtttta ttttctgctt
    49621 gaaatattcc atcgaaacat aagtccatac atcccgacaa aagtcgatta aaatggtatc
    49681 gattcgtttc aggctatcca acaaagctgc cagatgatcg taattcgaaa tttgggtagt
    49741 ccattcttca cgttgtatgc caagtttttc ggcaagaaat ttatcggcaa aatttttcca
    49801 ttcaatggaa ggatacgcta ccgaatgtgc attgaaattg ccggtggcgc ctccaaattt
    49861 tccggaaata ggaaccgcac gaagcagtga aagctgtttg tcgagccgat agacaaaaac
    49921 cattatttct tttcccaatc gcgtgggaga agccggctga ccgtgagttc tggccagcat
    49981 gggaatatct ttccattcgt cggccaactc gcgcattttt ttcaatagat tttccatttc
    50041 ggggtagtaa acatcttcca gcgctttctt tatcgaaacg ggaactgcag tgttgtttat
    50101 atcctgagag gtcaatccga aatgaataaa ttccttgtaa tcgctcaaac caagcaggtc
    50161 aaattgctgt ttcaaaaaat attccacagc cttgacatca tgattggtag tttgttcgat
    50221 ttccttgatt ttttcggcat ccgattcctg aaaatgaata taaactgccc tcagcttatc
    50281 gaaacaatct ttcgggaccg attgaagttc ttttaaagga atttcgcaaa gtgaaataaa
    50341 atattctact tcaaccatca cacgaaaacg aattaaagca aattcagaaa aatattcggc
    50401 atatttttct gtttttgaac gataacgccc atcaacaggc gatatggcag taagtggtga
    50461 aagattcata aaaattttaa aataagaatg agcggtttgt cggtaaataa aattggtttt
    50521 ttgtgaaaaa tcagtacaaa tttaatcatt ttcggcatat tattgaaagt ttatatcgta
    50581 ccttgtagca acaaagtatt ttaagtaaat ttgcaaactg agttctcttc gttcaaaata
    50641 tcccgacagg gttactttca ggcagttttc taaaattgta tttttaaaca aaaagagaaa
    50701 ttttttttgt tgtactattt tcaattgacc agagaaatct cataaatcat ttttaaacag
    50761 atgaaaaaaa gtatcagaat acataaagaa ggttggttta ttttgacagg attgttgcta
    50821 ttgctgtttg ctgtcaattt gattatttac atggaatttc ctggtccggc aatggccatc
    50881 aatacagttc tttctgcaat tattctctgt ttttttgttt ttttcttccg caatccgaaa
    50941 agaattgtgg aaattgatga tccgagtttt gtcattgcac cggccgatgg aacagttgta
    51001 gtggttgaga aaactttgga acccgaatat tttggcgacg aacgtattca ggtttccatt
    51061 ttcatgtcga tatttaatgt tcatgccaac tggtatcctg tttcgggaac ggttgtaaaa
    51121 accgaacacc ataacggacg gcatttggcc gcttatttgc ccaagtcgag cagagaaaat
    51181 gagcgttcta ctgttgtgat agaaactccc ggaaaaacgc aaattctggt cagacaaatt
    51241 gccggcgctt tggcacagcg cattgtaacc tatgctcatc cgggaaaaga atgtcatgta
    51301 aatgaacatc tgggatttat caagtttgga tctcgggtag atttgtttct gccgttggac
    51361 agtgaaattt ttgttcaggt gggagaaaaa actaccggca acgaaaccat tattgcacga
    51421 cttaacgaat aatttctgtt gtcatgactc tgcaggaagc tcagcaaata gttgataatt
    51481 ggatcaagac ttacggggta cgttattttg gtgaattgac caacatggcc attcttacgg
    51541 aggaagtggg agaattggcg cgagtgatgg cacgaactta tggcgaacag tcgtacaagg
    51601 cagaagatga aaagtatgat ttagctgacg aaatggctga tgttctctgg gttttgcttt
    51661 gcttggccaa tcaaacaggc gttgatttga cagatgcttt cagcaaaaat atcgaaaaga
    51721 aaacccttcg cgatgcacaa cgccacatac aaaataaaaa gctgtattaa tcatttttta
    51781 aattcacaac attatgaaag acagatatga agagttgtta gaactttacc agtgcgaatc
    51841 agttgacgac gaagccataa aaatagatat agacaatatt cttgcagaac attttgccga
    51901 aaatgcatca aaggatgtgt atgccaaatg tttgaatttg attgatttaa cttcgctcaa
    51961 tagtaccgat accgaaggag aaatcgtttc aatggttgaa aaagtcaatc atttcagagg
    52021 gacttttcct catttgtcca atgtggcagc catttgtgtt tatccggccc tgattccggc
    52081 cgtaaagtcg catctgaccg aagaaaatgt cgggattgct tccgtagcag ccggatttcc
    52141 ttcttctcaa acttttattg aagttaaaat tgccgaaact tctatggtgg tcaatgaagg
    52201 agcatcggaa gtagatgtgg ttatttccgt tgggaaattt ctggaaggca attacggcga
    52261 agtttacgaa gaactgtcgg aaatcaaggc tgcctgccgc aatgcacatt tgaaagttat
    52321 tcttgaaacc ggcgctttga aaaatagtgt gaatatcaag aaagctgcta ttctggcgat
    52381 gtcggcggga gccgatttcg ttaaaacttc taccggaaaa attcaggttt ccgccaccct
    52441 cgaagcaacg tatattattt cccatgccat aaaggaatgg tatgaaaaaa caggccaaaa
    52501 aattggatac aaaccggcag gtggaatttc taccacagaa gaagccgtga aacattatac
    52561 gcttgtgaaa cacattttgg gcgaagaatg gctcaacaat cggttgttcc gttttggtgc
    52621 cagccgattg gccaatcatc tgctcagttc gatagaagga aaagaaatca aatatttcta
    52681 atgctttaat aatcgcggtt cgacataaat tccgcaaaga gaatcatgga ctgtttcagt
    52741 tcaccttcag caaagccgtt gagttcttca atggctttgt ttttatattc ttccatcttt
    52801 tgcaaggtgt actccaagcc accgttttcc tgaacaaatt gtacaatttt ttcgatatgc
    52861 aagtcggaaa aatcactgtt ttcaatccat tgctcaattt cgtgcttttg gtggttagtg
    52921 gcgtttttta atgcgtaaat gagtggtagt gtaattttac cgtcttttat gtcgtttcgg
    52981 gttggtttgc caatctcatt gttttccgaa tagtcgagaa ggtcgtcttt catctggaaa
    53041 catattcctg catattcacc aaagtgatat aaatgcttca aagtattttt gtcggctttt
    53101 accgaaaggc ctccgataag agcacacgaa gaaaaaagca gggcggtttt tttttgtatg
    53161 atttctaaat aatcctcctc ggagatttga gcatgttgaa ctttgtccaa ttgtagcagt
    53221 tctccgtccg tgagattcat tcctacgttg gcaattgttt ccagaatttt tatattatcg
    53281 gcttttattc cagaactcaa tgattttgaa agtatatgat cgccaacaag tatagctact
    53341 ttgttgttcc actgagcgtt gatggatggt tgacctcttc gctggaaagt attgtccacc
    53401 acatcgtcgt gaattaagct ggctgtatgt atcaattcta atgaaattgc tccgtaaatg
    53461 gtactttcgg taacttcgcc gcacaacttt gccgacaata tcaccaagat aggccgaagt
    53521 tgcttgcctt ttcccaactg aagataattg ctgatattct ttaacagtgg attttcggtt
    53581 tgtaattcct ctgtcatcga tttttcaaac tgttcaattt ctttctctat gggctttcta
    53641 atcttttcta tcaggttcat aagaacagaa gtaaatgaaa taatctgcaa aagtaatcaa
    53701 atttaaacgt tcgttggtca taagctatcc aattatgcct tttttttaat tacttttaca
    53761 ctcaaataaa aaacgatttt tttctccatt tgtttggaaa atctttcttc tttgcatttt
    53821 ttaattttca gttagagaaa ttgcaataaa gaatttttcg tttttttcta attgactaca
    53881 actcactttt ttgggtgtaa aattcaaaaa cctatgaaaa aacttttttt aatcgatgca
    53941 cacggactta tctatcgttc ctattatgca tttattcgca gcccgcgcat caattctaaa
    54001 ggactgaaca cttccgctat ttttggtttt gtaaatacgc tggaagaagt tttaaagaac
    54061 gaacagccta cacatttggc cgttgttttc gatcccgaag gaaaaacttt tcggcacgaa
    54121 gcttacgaac agtacaaggc acagcgtgaa aaaacgcccg aagatatacg gaaagcgtta
    54181 cccatcataa aagagctggt tgctgcttat aacattcctg ccatagaagt taccggttac
    54241 gaagcggatg atgtgattgg tacacttgcc aagcaagccg aaaagaaagg atttgaagtt
    54301 tatttgctga cttccgacaa agatttggga caattggttt ccgaccatat ttttatgttt
    54361 cgtccccatc acaacggtgg ctttgaagta ctgggccctg atcagattaa ggaaaaatac
    54421 caactcgaaa atcaacaaca ggtgattgat ttattgggat tgatgggcga cagttcggac
    54481 aatattccag gttgccccgg cataggcgag aaaacggcag tgaaattgct tcaggaattt
    54541 ggcagcattg acaatttgct acaaaacacc gacaaattga agggctcgct taaaacgaaa
    54601 attgaagaaa acaaagactt gattaatttt tcccgatttc tggctaccat taaaaccgat
    54661 gtgccggtcg ctttggatga ggaggcactc aaagttgaac ctgccgacag tgaaaaactg
    54721 cgcgctctgt ttcaggaatt ggaatttcgt accttattga acaacaaatt tggcaatacg
    54781 ttttcttctt ttccgcccga ttatccgtta cttcaaaccc cttcagggaa taaaaaatct
    54841 tccaaagccg aacaaatgaa tcttttcgga gaaaccaata ggcctcaaaa aactgaaccc
    54901 gaaaaggaaa aaatactgtc gaaacctgaa gaaaatattg aagaaatttc accagttgct
    54961 tccaacggat ttctggcttt gcaaaatatt ccgcatcgct atcagttgat cgacgataaa
    55021 gtgaagcgtt cctaccttgt ttcgcaactt tttcttcaaa aatcggtttg ttttgatacc
    55081 gaaactaccg gacttgacgt tttttctgcc gatttagttg gtctttcgtt ctgtttcagc
    55141 gaaggagaag cttattttgt aactttaccg gacaacagag aagaggcaac aaaaattctg
    55201 aacgaattca aggcgttttt tctgagcgaa caaatcgaaa aaattggtca caacatgaag
    55261 tttgatctgc tgatgctctc caactatggc attgatgtaa agggaaaact tttcgatacc
    55321 atgattgccc attatctatt gcaacccgaa ttgaggcatg gcatggatta tctggccgaa
    55381 atttatctcc actaccatac cattcattac gaagatctgg taggagcaaa agggaaaaat
    55441 cagttggata ttagaaacgt ggaaatttcc aaacttacgg aatatgctgc agaagatgcc
    55501 gatgttacgt ttcgcctgaa acagatattg gaaaaggaat tggcggccaa cgagctcgaa
    55561 aaactttttt atgaaatcga aatgccgctc atgaaagtgt tggtaataat ggaaaaaaca
    55621 ggtgtacgca tcgacagtga agctttgcgt caaagttcgg aaattctaac ggaaaatatg
    55681 ttgaaactcg aaaaggaaat ttatgaaatg gcgggcggtg agttcaatat caattcgccg
    55741 gtacaggtgg gagaagtatt gtttgatcgg ttacatttgg atgacaaagc caagaaaaca
    55801 aagaccggac agtattctac ttcggaagat gtgctggaaa aacttcggca caaacatccg
    55861 attgtggata aaatattgga atacagagga ttgaaaaaat tgttgagtac ctacattgat
    55921 gcgcttcctc aactgattca tccgaaaacg gggaaaatac atacatctta caaccaaacc
    55981 aacaccgcca ccggtcgatt gagttcgagc aatcccaatt tgcaaaatat tcccattcgc
    56041 gacgaattgg gaaaagaaat cagaaaagcc tttatcccgg aggaaaacag cctgtttctc
    56101 agtgccgact attcgcagat tgaacttcgt gtgatggctc atttgagcaa cgaccaaaat
    56161 atgatagagg ctttcagaaa cggtttggat atacatgccg ctacagcagc caaaatttac
    56221 aaagttccgc tggaacaagt aacttccgaa atgcgtcgtc aagccaaaac agccaatttc
    56281 ggaattattt acggaatttc ggcttttggt ctggcggaac ggctcggtat ttcacgcaca
    56341 gaagccaaaa ctctgattga tgagtatttt ctagcctatc cgaagataaa aacttacatc
    56401 gaaagtgcca tcaaaaaagc caaagaaaac ggttatgtgg agacgctgct cggaagaaaa
    56461 cgctttttgc ccgatatcaa ttcacaaaac agcgtggtga gaggttttgc cgaacgaaat
    56521 gcggttaatg caccgattca gggctcggca gccgatatta taaaaattgc aatggtcaac
    56581 attcagcgaa aattggaaaa agaaaatttg caggcaaaaa tgattttgca ggtgcacgac
    56641 gaattgaatt ttaccgttcc tgtaaacgaa ttggaaacgg tgcgtgaact tgttgtttac
    56701 gaaatggaac atgctttccc tctcagtgtt ccgttaattg tagattgcgg cgttggcaac
    56761 aactggctcg aggcacatta aaattttctg tcggttacgg ataggttttt tgttttggct
    56821 acagtcaatt tacctgtacc cgcaaataaa taatgaaccc ctacttaaaa ccttattatt
    56881 ttcttaagaa gggagaaata agattttttg aagagtaatg ttcgattttt tgtcgtttct
    56941 tactttttat ccatttttta aaactctttt cccgagaaac ttttcggagg ggactcaata
    57001 attatatttt cgacaaaaaa ttttatcatc ttgccgttta cagccatttt ttccttttga
    57061 aaaataccaa gactcctacc gtgaaggaaa tcatcaataa aatggaaaaa ggatacccaa
    57121 atttccaatc gagctcgggc atgtatttaa agttcatgcc atacatgctt gcaataagcg
    57181 taggtggaag gaatataacg gaaaccaccg tgaagatttt aataatgttg ttttgctgta
    57241 aatttaccaa cccgagaaaa gtatcttgca aataatccag tcgatcgaaa tcaaaacggg
    57301 tatattcaaa caaagaatta atgtctttta gaatgatgtt aagacgcggt atcaaatctt
    57361 cgggaaagaa attgcatttc agcatgttgg aaactacacg ctgcttatcc acaatatttt
    57421 gtctgataat cattaccttt tcctgcaatt cctttatttg cagcaaaatg tcttgatcga
    57481 gatcggattc cgtattcaaa ttcttactta aattggtgat ttggtcggta acatcttcta
    57541 tcatgtcggc atcgtattct actcgcgttt caaagagaga aaccatcaca tgatagcctg
    57601 aaggaaaatt actggtattg acaaagattt tttttacggt ttcgttaaag gatttcagct
    57661 cggtattgcg aaccgaaacc aaaattccgt tttttatgat aaacgaaacc ggctgcataa
    57721 cataattgtc ctgcaaaaaa ttggaattcg ccacaatgct gtcatcggtc tgaatgtagc
    57781 gggaactcat ctcaatttct tcaatttctt catcttcctg aatgtaaatt ttaagaaatt
    57841 gctcgagttc tgattctata ttttcggaaa catcattcat gtcaatccaa ataatatctt
    57901 taaggttgat ctttttgagc gtatcgatac ttttggaaat tttcagtttc ccatcatttt
    57961 cgtggtaaaa aattcttaat tccgacataa ttttgctcct ttcttttttt tctgctgttt
    58021 cgaagcagca gtggtggcta ttttgtcacc ggataaacga attattgttt ttctttcaaa
    58081 gccgaaccaa ttttattggt caactcaaca aatgttttaa catccaactg ttcaggccgc
    58141 aaatcgaaaa gaggttctgc atacatcgaa tggctgggaa tgattgattt caatgaatta
    58201 cgcaacgttt ttcttctttg attgaaagcc gttttcacaa ctcgcctgaa tagttgttcg
    58261 tcatagtcca cttcgggatt ttctttgcgc ttgaggctga tgacagccga ttttacttta
    58321 ggtggagggt aaaaaacagt ttcgtctaca gtgaaaagat agttgatatc gtaatttgcc
    58381 tgcagcaaaa cacttagtat tccgtacgtc ttattgccgg gtggagaagc caaccgttcg
    58441 gccacttctt tctgaaccat acccacaaca gaaggaatta aatctttgtt atcgagaact
    58501 ttaaagaaaa tctgactgga aatattgtag ggaaaatttc ctattatgct gaaaggatga
    58561 gaaaaaatct gatttaagtc aagttgaaga aaatcttttt ctaaaatttg aagctggggg
    58621 aagtgttgct gcaggtaaac aaccgattcg tggtcaattt ctacaacttt aagatcaatt
    58681 tgtggctgct gaagcaaaaa ctgagtaagc atacccatgc ccggccctat ttccaatact
    58741 gaagcaggac tgccgacggg taaactgccg gctatacgac gggcaacatc catgcttttc
    58801 aggaaatgtt gacccaagta tttttttggt cttactaaag tcattactgt gataatcgtc
    58861 agacatatga gtatttaact gaataaataa aaatgagaaa aactaaaaaa tggaatttaa
    58921 attaattcgt atctttgtag aaattttttc gccacaaaat taatcaaaaa ttgtttgata
    58981 attttattta taaaaaaaaa ttgcccgaat tgaacattta attgttgtta atgagaaaat
    59041 tttttcgaaa cacaggaagc tcgattacaa aattaatcga ttttttttac ccgcctttcc
    59101 gtaaattcat gcctttacag ttttttcgct atggagtatc cggagctgca aacatggttt
    59161 tcgactgggt attgtatttt gtagtttaca atttcgtact tcgccatcaa atgcttcact
    59221 tgggttttat aaccatgagt tcgcatattg cggcacttgc tctgacattt cctgtttcgt
    59281 tcatgacggg tttccttttg caaaaatacg taactttttc tgcttcctat ctgcgaggtc
    59341 atgttcagct tcgccggtat ttgttggtcg tcattataaa tctgggcttg aattatgccg
    59401 gtttgaagtt ttttgtcgaa attgcccatt tctaccctac tccttctaaa atgcttacca
    59461 caattataac aacacttttc agctattttg cgcagaagaa attcactttc aaaaccgctt
    59521 ctctctcttt gaacaaagag tcgaatcatt aaaacaacat tttcttgttt caacctatga
    59581 aaaacatggc tgtaaccact aaaatgacaa ccgacaaatc gaagttgtgg aacattttca
    59641 agtggagttg cctgacggca gcattccttt ttttgggtta ccggctgatt tgctttcaac
    59701 attacgggcg attgctttct caattcaaag aaatatcatt ttggaaaact tggtatttgt
    59761 ggggtgtaat tttgttgttg cctgccaatt ggctattgga atcggtaaaa tggcaacggt
    59821 tagtgagaaa ttcggaaaaa ataaatatgg ctttggcttt tcagagtgtt atggcgggat
    59881 ttcttactgc cttcttcacc ccgaatcgtg tgggagaact ggccggcaga attgcctacc
    59941 tgcagcccga aaacaggaaa gcaggaataa cattaagttt tgttaacagt attacgcaaa
    60001 acatcataat ggctatttgc ggtattccgg cggccatttt attttttttg agtaataaaa
    60061 actctgaatt tgcaggagag aataattttt tcggatattt tctgatagtt tgcggaattt
    60121 tgttgttatt gagtggaatt tatttttctc tgcccccatt ggttgggcga ataaaacaaa
    60181 aaaagttgtc agcaaaatgg ataaatttta ttgcttgtct ggccgattat tcttttgccg
    60241 atttattaaa aattctgctg ctttcttttt tgagatattt ggttttctct tttcagtttt
    60301 atctgatgtt gcgatttttt ggcgtagaaa tcaacggttt ggctgcggca atagctattc
    60361 ccactactta cttgtttgta acttttactc cttctatggc tttttcagaa gctgccatcc
    60421 gaagctccta ttcggttttg gttatcggcg ccttctcggg tcaaacggtt caaattattc
    60481 tggcaagtac ttttatatgg gtcattaatt ttgtaattcc catgtttgta gggtctttcg
    60541 taatgatgca aacgcatccg ctgagatcga acaaataaat ttttacgtca ttttttctcg
    60601 agttccaaat ctattcttcc cagataaagt ccgcttttcc ccatctgtgc caatacaaca
    60661 ggtttaccgg ctgcattttt taagttggtt tgagtaataa tttgatgaga atgacctcca
    60721 atcatcacat caatatacct ggttttgcgt attatatcgt agtcggtcag ggtttgcttc
    60781 agcgtgtcgg cacccaaatg cgacaggcaa ataatcaaat cgcacttttc gcttcttttt
    60841 aaaaagttgg agatattgat agccgtttcc acaggatcga tgtaattcat tttcttgtag
    60901 ttattcctga aaatcagacc ttttggattg acattcaaag ccattactcc gattttcaaa
    60961 ccggctttgc gaataatgag gtaaggtttt accaaagttg ccatagcagt gctgtccatc
    61021 tgataattgg aagttaatat gggaaattga gcgtttttca acaccgttgc catagtgtcc
    61081 aacccattgt cgaattcgtg attccccaaa gttacagcat cgtatttcat tttgttcatc
    61141 ccatccacct ctattctgcc attgtaaaaa ttaaaataag gcgttccctg ccaaaaatcg
    61201 ccggcatcga acaacaacac atttttattt tgttgccgga tttgttcaat tattcccatt
    61261 cgacgagcat aaccgccttt atcggaaaca ggaagatttg atttttccgt cggctctatc
    61321 tgactgtggg tgtcgtttgt gtggagaatg gtcaacttta ttctttctcc tccataaaga
    61381 atactgttga acgccaacaa aaaaacaaga atcgcagtat tttggatagt tttcataaaa
    61441 aatagttttt agttttccat acttattctg ccatcaattt tggattgtat aacatgtcct
    61501 ttcaaagttt cattccgaat gaaatcgata aggacattcc gaagttttaa tccggtacct
    61561 gaaatttttt catattctga taaagccgtc atttggtcgt tcccttctgc gagataatca
    61621 ctggtagcta tggtataaat tttttccgga tcaatcggat tagtaccgac cagaatttgg
    61681 gtggctttgc cgttttttat tttcatcgag ataccggaaa ctccctctcc tccgacagaa
    61741 gcaaaaaatt ggaaaagttg aattaactta tctcctctga cccaaagaat taccaactca
    61801 ttttcaaaag gcattaattc gtagatattg cgaatggtaa tattaccggc aggaatttca
    61861 gttcgtaatc cgccaagatt taccacagac acatctacct cttgatgtaa aaaatccgaa
    61921 gctgcacgac gaaacacatc ggcacaaaaa tttgaaagtg gactttctgg agcaaaactt
    61981 ctcatatttt ctgcagaata gccaattaca acagccattt cagcatcaat tctctctttc
    62041 accggttgaa gagcagcctc atattctttg tcggcaaact tttcagtagt actgtcgata
    62101 ggtattttta ctgatgttgc ttccattacc tgccacgttt tggggctgca agagtaaaac
    62161 aagatattaa acagcacaaa taaagtacaa aaattaactt tcatcagaat ataatttatt
    62221 ttttaaaggt ctgatggaac ccacctgtct gaatattata ttcattcgta gttgtgtacg
    62281 caatacatgg gggtttcata ataatagatt gatttcaaaa tctcttttct ctcgtgtttt
    62341 tgcatgcaaa ttataaattt tattccaatt ttgctcttaa atattgttgt ggccattttg
    62401 agttttcttt tacatgccga gcacaatgaa gagcaaaata gggatttcgg agtaattctc
    62461 ttcccaaaag cactatatcg gctttttcgg ttcgtagaat atcttgtatt tcttcatgct
    62521 tggtaattaa tccaactgct gccgtaagaa tgcctgtttt acggatttgt tccgaaaatg
    62581 ggacctgata acaaggagct aagggaatat gcacatttgg taaaattccg cccgacgaac
    62641 aatcaattaa gtccactcct tccagataaa gaattttagc caactcaacc gtatcttcca
    62701 aacaccatcc ttgatcagtc caatcggtag ctgaaagcct cacaaaaagg ggcaatcttt
    62761 caggccaaac ggtttgaacg gcggaaacaa tttcctgtaa caaacgtatg cgtcctttaa
    62821 aagaaccgcc gtattcatct ttcctaaaat tactgattgg cgaaagaaat tcatgaataa
    62881 ggtacccatg tgcagcatga atttcaagaa catcgaatcc agcttgaaac gccctcagtg
    62941 ctgcatcttt gaactgatta actacctttt ttatttcaga cttggtcaat tcatgtggag
    63001 gaatttcttc aggacgaaag ggaatagcag aaggagcaat cgtttgccag ccgccctctt
    63061 tattggtgag ctgtttttca cctttgaaag gagaagaacg agagcctttt cttccggcat
    63121 gggcaatttg aatccctgct tttgaatcct gttgatgaat aaaatcaact attttcttca
    63181 atggttcgat ttgttcatct ttccacaatc ccaaatcttc gggcgaaata cggccaacag
    63241 gactaactcc cgttgcttca aaaattatta aagctattcc tcctacacct ctcgttccat
    63301 aatgaaccag atgccaatcg tttgccaatc catcttgtgc agaaaattgg cacatgggtg
    63361 acatgcccag acgatttttt aaagttatat cctttatgcg aaatggttga aaaatttgca
    63421 acataacaca aataattata tcttggtttg atttttgaaa aggaaacagt aaacgattaa
    63481 caattgtttt atacaaaaca acaaaaaaac atcgaattgg taaaaaaaat tcgtattttt
    63541 tatatatcgt atcagaaaaa cttttcgaaa tatttagcaa aaattacaaa gacaaaaaac
    63601 actccataaa aactatattt acaaaaaaca tttatctttg cataaactca atcattattt
    63661 caatgcttaa acaacaatta caacaaaaac ttcaacaaaa actttcaccg gcacaaattc
    63721 aagtaataaa aatgcttgag attcctactt tggaacttga agaacgtatt cgtcaggaat
    63781 tggaagaaaa tccagttttg gaaataggct tagaacccga agaagaaaaa aaggaattgg
    63841 atgactttgg tgaagattac gaagaaacag gaaatgacga gggagaaagc aacgatgaaa
    63901 ttgattttga agaatatatg tcggatgatg acatacccga ttacaagtta aaggcaaaca
    63961 acacttcaaa agacgacaaa tacgaagaaa ttcctttctc cgccggttcc acttttcatg
    64021 atttcttaat ggaacagttg aatcttgttc cgctttcgga gagggacagg gctttggctg
    64081 agtttgtcat aggcaatatt gatgaagaag gttatttgcg tagaacacct gaagcattgg
    64141 cagatgatgt agcctttcag gccaatatgg acactaatgt aaaagaaata gaaaaaatcg
    64201 ttaaaattat tcaacagttt gatcctccgg gcgtaggagc ttcctcttta caggaatgtc
    64261 tgttacttca gttggaaaag aaaagaaacg agaaggaaat tgaaatacca aaacgcataa
    64321 ttcaagacta ttttgaagaa ttttcaaaaa agcattacga taaaattata aacagcctcg
    64381 aaattgacgg taatacacta aagaatgcca taaacgaaat tattcgtctg aatccgaaac
    64441 ccggcaatgc atggagcggg aacatcttgg aaaaaaacat gtcgattatt gtacctgact
    64501 ttattgtgga aaataacgat ggtgaactaa ccgtacattt aaacaacagc aatattccct
    64561 atttgcgagt caatgctgct tattccgaaa tgtttcagga ttatacttcc aacaaggaaa
    64621 atcagacacc tcagatgaaa gaagctattc tgtttgtcaa acaaaaaatc gattctgcac
    64681 gttggtttat tgatgccata aaacagaggc agcagacatt attgactacc atgactgcca
    64741 ttgtcgaatt tcaaaaagat tttttcatcg aaggagatga aatttatcta aaaccaatga
    64801 tacttaaaaa tatagccgac agaaccggct atgacatttc tactatttca agagtgagca
    64861 acaataaata tgtgcaaaca gaatttggag tttttcctct gaaatatttc ttttctgaag
    64921 ccatgaccaa cgatgccgga gaagaaattt cgactcgaga aataaaaaaa attcttcagg
    64981 aatgcgttaa taacgaagat aaacgtcatc cgttaaatga tgaaatgctg gctgaagtat
    65041 taaaacaaaa gggctactta atagcccggc gtacggttgc taaatatcga gagcaactaa
    65101 atattccgga ggctcgatta agaaaagaaa tatgaattta aagatttgtc gtttttaaaa
    65161 aaatataaca ttatgaaaat cctctataaa attatttcga ttattttaca gcctatattg
    65221 ttgccaactt atggcatatt gctgctgata caaattcccg tttttgcaaa ccttccgatg
    65281 cagtggaaag ccattgctgt tggaggaaca tttttgttca ccactttgtt gccgtctata
    65341 cctgttgtta taatgtactt taggaaggaa gtgagcgatt tgtttatttc caatcgcgaa
    65401 gaaagaaccc taccttatct gttttccttt ttggcttatg tgttctggac acttttcctg
    65461 gtacgtacca tgcacttgcc aatgtttatt ggcggattgg gcattgggtc ttccatatcc
    65521 attcttctta ttacttttat caatgtcaag tggaaaatca gtgctcatct ggcaagtttg
    65581 ggaggtttgg ccggaggaat attcggaatt tgttaccgat attccataaa tcctctttgg
    65641 ctttttattc ttatactgct tttggcagcg ctttctgcaa tttcacgtgt tgctctcaag
    65701 gctcacactc caatgcaaac catagccggt tttgctttgg gttttttatg cgttttttta
    65761 cctgttttac tgctttaaaa aaatcttctt tgcaggttaa ctaaaattct atcgcaacaa
    65821 tagatctgaa agaacgtccc ggcatgggaa aacctcgaac aatttcataa tttttgttga
    65881 gaaaattgtt tatttccagc ttgaagaagc aggaataaga tgatttttta aaacttcgtg
    65941 aaagcgacaa attatgatcg gcataacccg gcaaacggtt ggaagcataa ttttgaaaca
    66001 aggtataccg atgccctgac cagataaatg aataagaaag attaacccaa ggcatttcca
    66061 aagcagcttt tccggaaccg gaaattcttg gcgtatacgg aatttgatgc aggtaatctt
    66121 cggcgttagg atcagtaaca ttcagtgctt gctggtaagt atacgatcct ccaaatatca
    66181 gtccaatatt ccttgttatc gaaattccgc tttccattgc cacatccaac ccattgatag
    66241 agactttacc acaatttttc atcgtccagt taaaaatatc cttgttagga aatgcaacaa
    66301 ttttatctct aatggcattg tgaaaggcat ctaatgttac tgaaagtaat gaaatatttt
    66361 ccgatgaaaa agaccaagta atgccggcat tgaattgttg cgcattttcg ggctccaaat
    66421 ttctgttgcc cacgtatgaa taataaagat cgttgaaagt tggcattctg aaaacatttt
    66481 tataaaaact gcgtatcctg aagtcatact gttcaaaagg tttaacagac aaactgaaaa
    66541 aaggtgaaaa atgctttttg tcgttgggag atgatccaaa cttaactttt tcattcacaa
    66601 atgtttgtaa accaccagca gtagccaaaa attgattgga tacatatttg gcgtttaaaa
    66661 ccatcaatac ggtgtttctt ttcggaaagg caaattccgg caagttggca ttcagcgtat
    66721 tcaacgaata atctgttgat gctgaaaaag ataaattttc gaaagccttg taaaggaacg
    66781 aagcagaaaa gtaaaattcc tgttgtgaat atgtattgtc tatttttcct cccgaattgt
    66841 tataatttgg atcgacataa tgcaaataac cattttgata tttggtgttg gcttgaaaag
    66901 ataacaagga agaaaagcta ctttcataat gtgcctgagt aaaaaaagtt tgatctttgc
    66961 ttcgctgatg gttggtattt tcgttcatgt aataaataac tgcccccggc aagcctcttt
    67021 ctgattcata aaaataggtt ttcagatagc ctgaaccacg atgaaaaaaa ttggcaaaaa
    67081 ttgttccttc caatcgcaag gtttcaacat cggtattttt tcttgtttct attgaagaac
    67141 tgtcccctgt ctgcgtacca taaatcagca gataaggata ctcaccctca gcagtaagcc
    67201 attcaccact aaaaacggca gaaatttttc tgctcatttg ctgtcgtata aaaagagaag
    67261 gattaataag accaaaagaa cctactttac aggtaatttt tccgtgtatc gatttttcct
    67321 cttcaaatcg tggttgtaag gtgcgaatat ttaacacgga agacgaagca aaagaacgag
    67381 cgggaagaaa aatgttgtcg ctttgtccat tatttaatga gaccatatcc acattgtcaa
    67441 gcgaaaaacg cccaagatca atttgccccg tctgataatc ggttagcatt atcccatcat
    67501 aactcactgc tgtatgttca gcacccagac ttcgaactga gacagttttc aaaccgccaa
    67561 tacctccata atcttttacg ttgacacccg caaaatattt tatagcatca gaaagttgca
    67621 atacattcag attttctaat ttttttgccg aaagaatctg caatggcata gctgaacgaa
    67681 tttcgctgtt ttgaaatttt tcaacaacta ctatttcggg aatggaatat gtttttgccg
    67741 tatccatacc catatcttga aataatatct ctgctcttac aggaataaaa aaaaacagaa
    67801 aaagaatttc aatcagataa aagaaaaaat ttttgtgcat aaccggtttg taatcattta
    67861 caaaaacagc ctcaatagaa aaagagaatc aacataaata aaaatgtgta gatggttaaa
    67921 gctctttcct cgaaagcttt ttaactgaat aaaagcatgg caggtcttct ggcttgctct
    67981 tttttttgaa cgccttcccg taatgggaaa ttgaaaaaaa gatatattat ttttttcgac
    68041 aacccctgaa cagtggcaac agtattgttc aaaaaaacta caagagctta cagcagcggg
    68101 actgcagccg attgacacgg ctttcccttt tcatcaattg accggatatg gttgattgaa
    68161 accaatgctt ctgcaaagat acatgaaaat ttcaaatcga cgcacagaca aatattagtt
    68221 aattcagatg gaaaagagct aacaagtcaa accatcaaca tgcagaaatc cgctaaaata
    68281 aacatacata agaatttatt ttacagttaa atgcatgtat ttttcggttt gtaaaaaata
    68341 cattttaatg gaaaaaattg tttgctacat atggtcaact ccaattattt gggttaaaaa
    68401 acacaaaata tgggttgctt tcaggttggg ttttttt
//
LOCUS       DAOSCH010000002        64997 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_353_length_64997_cov_7.503003, whole genome shotgun sequence.
ACCESSION   DAOSCH010000002 DAOSCH010000000
VERSION     DAOSCH010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 64997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64997
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_353_length_64997_cov_7.503003"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..1538
                     /locus_tag="PLZ54_00255"
     CDS             315..1538
                     /locus_tag="PLZ54_00255"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOJ65403.1"
                     /translation="MNKEEIEHIRADFPILSEKVYNKDLIYFDNAATTQKPRCVVEKI
                     EYAYFHLNANIHRGVHYLSLKATEAHEAARLTAAEFLHAEKKEEIIFTRGTTESINLV
                     AFSFGEAFCHPGDEVIVSAMEHHSNIVPWQMLCERKGMKLRVIPVNENGELDMVAYSS
                     LLNEKTRIVSVAHVSNVLGTINPVKEIIRIAHEKNIPVLIDGAQAVPHLSVDVRELDA
                     DFYVFSGHKIYGPTGIGILYGKEKWLNAIPPYQGGGEMISTVTFEKTTYNELPFKFEA
                     GTPDYVGSTALAEALRYINNIGLDNIAAYENELLHYAEQQLMEIENMHIIGTAKNKCS
                     VLSFLVDGIHPYDIGMLLDKLGIAVRTGHHCAQPIMDMFGIPGTVRASFAFYNTKEEI
                     VRFTEALKKVVNMLK"
     gene            1606..2028
                     /locus_tag="PLZ54_00260"
     CDS             1606..2028
                     /locus_tag="PLZ54_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOJ65404.1"
                     /translation="MTINELQDEIIEQFSQFDDWMDKYALLIDMGNSLEPLENEYKTP
                     QNLIEGCQSRVWLDAQMKDGKIYFRGDSDAVLVKGIVYLLIHVLSGHTPDEILNADLY
                     CIDKIGLREHLSPTRSNGLLSMLKQMKMYALAFKSKQE"
     gene            complement(2035..2781)
                     /locus_tag="PLZ54_00265"
     CDS             complement(2035..2781)
                     /locus_tag="PLZ54_00265"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOJ65405.1"
                     /translation="MIYFLSDAHLGSRLVENPREHEQKLVRWLEQVRRDAEAIYLLGD
                     IFDFWFEYKTVVPKGFVRFLGKLAELSDQGIEIHFFTGNHDIWTFGYLEEEIGMKVHR
                     EPLTVKLGNKLFYLAHGDGLYTNEPKFLFIRKIFHNSFAQRLFQLVPPRLGQKFGYWW
                     SKKSREKVLSLDNKYLGEENENLVIFAKNYILNHEVDFLVFGHRHIALDLQLQQQKRV
                     IILGDFVGIFSYGVFDGENFRLEYFEPQGE"
     gene            complement(2859..3173)
                     /locus_tag="PLZ54_00270"
     CDS             complement(2859..3173)
                     /locus_tag="PLZ54_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HOJ65406.1"
                     /translation="MSDFLETEEKIVQMLRTVYDPEIPVNIYDLGLVYNIDVDEDGHV
                     EIDMTLTAPNCPVADFIIEDVRMKVESIEGIKDVDVNIVYEPAWDKSMMSEEAKLELG
                     LL"
     gene            complement(3268..3687)
                     /locus_tag="PLZ54_00275"
     CDS             complement(3268..3687)
                     /locus_tag="PLZ54_00275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOJ65407.1"
                     /translation="MTYTYELEMKVRDHECDLQGIVNNAVYQNYFEHTRHEFLQANHL
                     SFSELHQQGIDAVVARIEIAFKKPLLPGEVFLSKLYLRKEGVKYSFYQALFKKAEGTL
                     CAKAKVDTVVVINGKLASAFEPFEKLLADNHAVKGEE"
     gene            complement(3693..4319)
                     /locus_tag="PLZ54_00280"
     CDS             complement(3693..4319)
                     /locus_tag="PLZ54_00280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOJ65408.1"
                     /translation="MSLEDYILQHSDSEPAYLTKISRDTHVKLLNPRMVSGHLQGRVL
                     SMLCKMIQPHKALEIGTFTGYSALCIAEALTDEDVLHTIEIDDELEDLILENFAGSEH
                     GHRIKLHIGNALEIIPTLNEVFDLVFIDADKREYTAYFEAVFPKLRNGGFILADNTLW
                     NGKVVTEAKPNDRQTMEILRFNDLIASDDRIEKVILPLRDGLTIIRKK"
     gene            complement(4534..7029)
                     /locus_tag="PLZ54_00285"
     CDS             complement(4534..7029)
                     /locus_tag="PLZ54_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ65409.1"
                     /translation="MKIIHYLLLTCILASSCSRQVSLREVIDFTQDWKFTLSDSAMDA
                     SSPAFDDGAWRTLNLPHDWSIESDFSEKFPATAGGGALPGGIGWYRKTFFVPQSEQNK
                     CVYVDFDGVYRNSEVWINGHYLGKRPYGYISFRYELTPFLKFGEKNTLAVRVDNSKQP
                     NSRWYSGSGIYRNVWLTITQPIHVDLWGTYFTTPVVNENAATIQIQTSVRNSTGERRL
                     VKVEQVLLDNEGRKITKNETTIEVLPDSLAVASQSFSVNKPILWDIEHPYLYKMITKL
                     SVEGKETDEYETSVGFRYFNFDAQKGFSLNGKHLKINGVCNHHDLGALGAAINKRALE
                     RQLQLLKDMGCNAIRCSHNPPAPELLQLCDSMGFIVMDEIFDMWFKKKSAYDYAQDFP
                     KWYKTDMTDWIKRDRNHPSVFMWSIGNEVMEQWEDNITTDNMNLDQANLLLNNLKISD
                     SPKAKISESSLLTKHLSDIVKLLDPTRPVTAACNGTQKNNPLFLSGGLDIIGFNYHHN
                     EFMDVPKNFPGKPFIVTESVSAVQTRGFYLMPSDSIYIWPESWDKPFFNPLQKCSAYD
                     NSHVPWGSTHEKTWKIVKKHDFISGQFIWTGFDYLGEPTPYSWPSRSSYFGIIDLAGF
                     PKDIYYMYQSEWTDKDVLHVFPHWNWQKGQTIDVWAYYNNADEVELYLNGRSLGKRDK
                     KGDDLHVMWRVKYEPGTLKAISRKDGKEVLAYEVKTAGAPVKIRLTADRSTISSNSKD
                     LSFVTVELLDKDNNVHPLADQLVKFTLKGPGMIAGTDNGNENDSISLKKPQRHLFFGK
                     CMAIVQSTEKKGKITLKATVDGLPDEQIVITSK"
     gene            7469..8077
                     /locus_tag="PLZ54_00290"
     CDS             7469..8077
                     /locus_tag="PLZ54_00290"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOJ65410.1"
                     /translation="MLIKIYETNPNPHEIHRVIETLRDGGTIIFPTDTVYAFGCSLFN
                     KHGIEYIARIKKKDLRSSQLSFVCESIKQISEYTKLDDETFKLIKKNLPGPFTFILKG
                     NHRLPKLFKDKKTVGIRMPKNNIALTIVHELGNPLMTSSIFYHNNSLEYIVDPEMIEE
                     KYGHLVDLVIDGGPGGYIPSTIVDCTCEEPVILRQGAGELIW"
     gene            8853..9392
                     /gene="hpt"
                     /locus_tag="PLZ54_00295"
     CDS             8853..9392
                     /gene="hpt"
                     /locus_tag="PLZ54_00295"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOJ65411.1"
                     /translation="MEIVQIKDKKFKKFIPEQEILSSIRKIGEEINKTLKDKNPLFIS
                     VLNGAFMFTSDLMKVVNIPCEITFIKLSSYEGLYSSGSVKEVMGLNENIVGRNVVILE
                     DIIDTGLTMEQIIDSLKAKGAGEIRVATFLQKPDALQRNIKIDYIAMKIPNEFIIGYG
                     LDYNGYGRNLKDVYTLITD"
     gene            9413..9994
                     /locus_tag="PLZ54_00300"
     CDS             9413..9994
                     /locus_tag="PLZ54_00300"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018106710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOJ65412.1"
                     /translation="MLNIIICGAPGSGKGTQSKLIAEKYNLKHLSTGDMLRNEIKLQT
                     EIGKKAEQFISRGQLVPDEMIVAMLDHVLDKEMHHYKGIILDGFPRTFAQAEALEKLL
                     KKRNEEISLLLDLKVNDEELISRLLKRAEISNRSDDKLDVIRKRLEIYYQNADEVNNF
                     YKKTNKYVAIEGVGTVEEIFSRISQIIDSKINN"
     gene            10030..11181
                     /gene="obgE"
                     /locus_tag="PLZ54_00305"
     CDS             10030..11181
                     /gene="obgE"
                     /locus_tag="PLZ54_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOJ65413.1"
                     /translation="MSGSNFVDYVKIFCRSGKGGAGSTHFHREKFIPKGGPDGGDGGR
                     GGHVILRGNKNYWTLLHLKYARHVFAEDGENGSKNRSFGKDGNDKIIEVPCGTVAYDG
                     ETGEFICDIQEDGQEFVLLQGGRGGLGNWHFKSATQQTPRFSQPGEPRKERTVILQLK
                     ILADVGLVGFPNAGKSTLLSVVSAAKPEIADYPFTTLVPQLGIVAYRDNKSFVMADIP
                     GIIEGAAEGKGLGLRFLRHIERNSILLFMIPAEEVDIREKYNILLNELKKYNPSLLEK
                     SKILAISKSDLLDAELKEEMRKNLPADVPTVFISSLTGEGILELKDLIWDELNKPSNQ
                     IIEISHAPIPISNQENEEELEEDFFPDNISSDMEETDRFEKMDWEDWDE"
     gene            11376..13100
                     /gene="recJ"
                     /locus_tag="PLZ54_00310"
     CDS             11376..13100
                     /gene="recJ"
                     /locus_tag="PLZ54_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOJ65414.1"
                     /translation="MTNKWIFKSLSEEEKIIQKNLVESLSINPTLAQLLVQRGIQTFE
                     EARSFFRPDLAELHDPFLMMDMDKAIERLNKAIDNKEKILIYGDYDVDGTTSVALVYQ
                     FLKKYYSDIDFYIPDRYTEGYGISFQGIQFAAEQNFSLIIALDCGIKATEKVKHALNL
                     GIDFIICDHHTPDKELPPAVAILDPKREDCPYPYKHLSGCGVGFKLLQAFTMKKGIDF
                     SELTPLLDLVGLSIASDIVPITGENRILAYYGLKRLNSLPSLGIRSIINVCGLADREI
                     TISDIVFKIGPRINASGRMKSAREAVELLIANDAEIARQKSGNINEYNNDRKDIDKNI
                     TDEAISIINSQDVYKKRKSIVVYQPHWHKGVIGIVASRLSDEYYKPTVVLTRSNGLIS
                     GSARSVPGFDLYKAIDSCRDLLENFGGHMYAAGLSMKEENIQAFTERFEQFVSENILE
                     EQTYPQLEIDAVLEFKDITPKFFRILKQFAPFGPENMKPLFVSKKIFDYGTSRLVGKE
                     QEHLRMELIDSSSENVMNGIAFGMHEYNDHLKALHPLDICYTLEENNFNGNSTIQLMI
                     RDIKTEEY"
     gene            complement(13138..14310)
                     /locus_tag="PLZ54_00315"
     CDS             complement(13138..14310)
                     /locus_tag="PLZ54_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007667358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOJ65415.1"
                     /translation="MMEQTERPYTFDRVVRLVITLTVLTALFFLTQRLSGVLLPFLIA
                     WLLAYLLQPIVHFFQYKLKFKYRFVAIFATLLLFIIILTGLGFLLIPMIINEIEKAVV
                     LINSFFRNVDVNSFLPIAWQNQLSEYFRQLDLQTLLGSEKFMSLVQKLLPEVWNFVGN
                     SLNFILGLAVIVIIFLYLFFILMDYEKISEKWIELVPMKYRPLITNIVQDIQDGMNRY
                     FRGQALIAMCVGILFMIGLSIIRLPLAIVIGLSIGILNLVPYLELLGIIPCAFMALLK
                     ASETNQNFWMIALSIAAVWLIVQSIQDLILVPRIMGKYTGLHPALILLSLSIWGSLLG
                     FAGMIIALPFTSLILSYYKRFIIKGENIEHPNVPQPEQTVSPVSSEECVSEEPKQD"
     gene            complement(14331..14921)
                     /locus_tag="PLZ54_00320"
     CDS             complement(14331..14921)
                     /locus_tag="PLZ54_00320"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOJ65416.1"
                     /translation="MFYSEQTSHASRRGSIEVICGSMFSGKTEELIRRLKRAQFAKQR
                     VEIFKPVIDTRYSESEVVSHDRNAIRSTPVGSSGSILLLSANVDVVGIDEAQFFDDGL
                     VQVCNQLANQGIRVIVAGLDMDYQGVPFGPIPALCAIAEDVFKVHAICVRCGALAYVS
                     HRLVDSNKRVLIGEMQEYEPICRNCFNEIQRSQMKI"
     gene            15108..16001
                     /locus_tag="PLZ54_00325"
     CDS             15108..16001
                     /locus_tag="PLZ54_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65417.1"
                     /translation="MNKDLILALLHKNIQDLDMLTESFMEMNELPPAILHLAMQKVDE
                     IHQYLEELNENNSTKKPAGKEIFPSEDLKDKKENNFLHFSEHSTNDFAEPSENVSQQT
                     EIEAIKEMEFQVGEENIAQETEKTEFSFTESPINEKLTEWDETEKETAEIEQEPLENE
                     KNRFVSTQPEIGGETTEITDSEAQEKEEPSANEGQKTAPTSIPLQEKFSKIPDNSLGT
                     TFANQKIDDIKKAISIGDRFRFQRELFRGNGEDMNKTLNYLNQLATLEEAVSFLQAKY
                     KWDGENETVQDFFQILKRRFL"
     gene            16129..16872
                     /gene="rsmI"
                     /locus_tag="PLZ54_00330"
     CDS             16129..16872
                     /gene="rsmI"
                     /locus_tag="PLZ54_00330"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOJ65418.1"
                     /translation="MSQQKNKTSLAGTTINNFSASKEGGKLYVVPTPIGNLEDMTFRA
                     VKILKKVNLILAEDTRTSSFLLKHFGIETPMQSHHKFNEHKTVENIVQQLKSGQILAL
                     ISDAGTPAISDPGFLLVRECVENGIEIECLPGATAFVPALVTSGLPNDRFCFEGFLPV
                     KKGRQQRLKELETEKKTMIFYESPFRLLKTLTQLSEVFGENRKASVSREISKMFEETV
                     RGTLAELIQHFTEKTPKGEFVLIVAGFEE"
     gene            17003..17896
                     /locus_tag="PLZ54_00335"
     CDS             17003..17896
                     /locus_tag="PLZ54_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65419.1"
                     /translation="MNKNISYRNIRVFSVIFLVAFLTVSCGKQSSDYKKLKAENDSLL
                     KEKERLEREVDDYFSSINQIQENLEKIKSTENVISVEPAGEELSQDARTRINEDINYL
                     NELLQTNKDELARLKSRLSKSSLKLTELERTITRLTKTLEEETARIAFLEKKLAEKDT
                     LIAQMGSTISQMGENINTLTTENKSKEEKIKQLDENLHSAWYVFGSRKELIDQKILTS
                     KGIFRKAEVLKDSFNKDYFVRIDARNAKTIPLYASKAKILTTHPTSSYTIEKQDGKLV
                     LIIVDPTAFWSVSKYLVIEVD"
     gene            18020..18727
                     /locus_tag="PLZ54_00340"
     CDS             18020..18727
                     /locus_tag="PLZ54_00340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOJ65420.1"
                     /translation="MNNKHPYRYVFLDLDDTIWDFHANARLSLQKMYEQQTLNRYFEN
                     FDEFFRIYAMRNMELWEMYGKGEITKDFLMFERFRYPLAKMGLDDLQLAEAIGKQYLE
                     ILPEQTTLLPFARELLDYLHSKYPLTLISNGFVEVQYKKLKSCQIEHYFSHVVLSEQA
                     GALKPDPEIFNYALHLNQASPEEAIMIGDSFSADIVGAKNAGIDQIYLQKNNMLPYTK
                     QQATFIVKSLEEIFSIL"
     gene            18848..19180
                     /locus_tag="PLZ54_00345"
     CDS             18848..19180
                     /locus_tag="PLZ54_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65421.1"
                     /translation="MNTNEDNLLEYSDEEAVKFILKSLPAEVKDRIDEDTVQYVLDVI
                     YDFYNEKGYIDEDDTAEETSIDEEEMYEYILENALKDEINLTEDDIQMILDAEFDYGK
                     SLGIYREE"
     gene            19292..20173
                     /locus_tag="PLZ54_00350"
     CDS             19292..20173
                     /locus_tag="PLZ54_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ65422.1"
                     /translation="MIYPLLWLLSLLPLKVLYAFSPLMYFLIYRVAGYRKKVVRQNLQ
                     NSFPELSASERRKIEKNFYRFFADLLLEIVKLLHISDEELLQRMKFVNVEEFFECCRQ
                     GKYPILMLGHYGNWEWILTISLLMPENCESFTVYKRLKNRKFDQFMKSLREKRGAVMV
                     EKRNLARSMIRWAKEGKFGAYGFIADQSPSGKNLRNWTTFLHQPTAILPGAEELARKF
                     NYPVFYARVSRPKRGYYSCEILPIAREPRTTSDNEISTRFMQLLEEDILAHPELWLWS
                     HRRWKIKPLIDEVKKDN"
     gene            20324..21358
                     /locus_tag="PLZ54_00355"
     CDS             20324..21358
                     /locus_tag="PLZ54_00355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ65423.1"
                     /translation="MQVSVVILNWNGKNYLQQFLPILLEKTRLSYVEIVVADNGSTDG
                     SLQLLKEQFPMVRIILFDKNYGFAEGYNKALSQIKSDYYVLLNSDVEVTDNWLLPLVD
                     YMEKHPQTAACQPKILSYFNRTHFEYAGAAGGFIDYLGFAFCRGRIFGEVEEDKGQYD
                     DPINVFWTSGACMMVRAGLFHKMGGFDADFFAHMEEIDLCWRFNSRGYQLTCVPESMV
                     YHIGGGTLQSEHPFKTYLNFRNNLLMLYKNLPKKQLKSVFFKRAILDYLAVFQFLASA
                     KPKNARSILKARSDFRKMKPNFLDKRKENILFSTATSFPMIYPRSIVVDYYLRKKKKF
                     TTLKFDQKPN"
     gene            complement(21367..23733)
                     /locus_tag="PLZ54_00360"
     CDS             complement(21367..23733)
                     /locus_tag="PLZ54_00360"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOJ65424.1"
                     /translation="MMGILLTEVLFVAGLQAQETIVVGQVIDKYDKHPLSSVSIYFKG
                     TSVGTTSNEEGYFLLRHTGKENILVFSAIGYKKTEIKIKPGSNIGLTVEMEESNTLLQ
                     EVFVLPGANPALDLMRKVRLLRKVNDETKLPHYEVLATEQNAIFLSRSQQRSISKQIF
                     LQLQEGNLLSSDSSLLVPLYMDEIKYQLTSTAKTELSKKIFSSSEEGNKILEQLFGEL
                     NPSLNFYENSIPVFNKNIISPLASIGNMYYNYYLADSMVTSSGKQYEIHFRSRNPRNP
                     AFNGKFCLDSASLALTYIEAELPSEANINFIRHLSIKQEFVPFSSKRWVRNKDQLSLV
                     LNLDILTDSVPAGSQIFFKRSTIYNTGSIQIPESFAQSGYSEETLNQKLQQLGKTPLM
                     NAAKWLADIIFTGYIPVGKIDIGKIQYLARTTDVEGLRLTLPFRTNEKMWKNFTVGGF
                     VGYGFKNEAIKYSGFTQYKLPAEKRRIIGLNYTNDYRRIDYNYNNFLYLENPLVSGDE
                     DIAGTIFALRSAGRLNERKEWTLSFSGDWNRNIESGLYFRSLQQLPSDALPLKKDGVD
                     IGNFQQQSFTFSTRFSFNEKKYEDHLQRIYINNRNPIIYGILEVGKYELGNTEKYYGK
                     VSGILKQNVQFDLGQVNYWMEAGSIFGSVPYPLLAYQPGAEGGYGFYRFSMMNFMQFA
                     ADKYFDFQGELIMNGLILNHIPLLKYLNMREMFSVKFGYGKMSNDHLSVMDFPAYVYP
                     FNRPYTEVGVGLANILHLFNLQAIWRLTDIKQFSGDNFAIRGSLRISF"
     gene            complement(23759..24682)
                     /gene="xerD"
                     /locus_tag="PLZ54_00365"
     CDS             complement(23759..24682)
                     /gene="xerD"
                     /locus_tag="PLZ54_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOJ65425.1"
                     /translation="MNSLLTDYHYYLKFEKALSENTIQAYERDLEKLNSYLSDFHVLL
                     QDATIELLRDFIIEIGSLGIHPRSQARIISSISSFYRFLVYKNIISTDPSELLESPKI
                     GLRLPQILSVEEIDAMISAIDLSKPEGQRNKAIIEVLYGSGVRVSELINVKLSQLYLN
                     EGYMMVEGKGSKQRLVPLSPNSIKQIELWKIDRNHLEIKKGHEDFLFLNRRGEKLTRA
                     MIFKIVKDLATMAGIQKNISPHTFRHSFATHLLENGANLRAIQQLLGHESITTTELYT
                     HIDVQFLRETILKYHPLNQSKNEENKNLSDF"
     gene            24872..25657
                     /locus_tag="PLZ54_00370"
     CDS             24872..25657
                     /locus_tag="PLZ54_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HOJ65426.1"
                     /translation="MLCKVKTLFVILFCAINFALSAYDVTGHRIIASIAYQNLNAATR
                     NRVDKLLGKQGIVYEAGWADEIRSDDNYAYSYQWHYQNLKDSLTSKDLKFLLEQPKAE
                     GKHLFFALDSLSGELKKNPNNAEALKFIVHFVGDLHQPMHLGRVDDLGGNKIMVKWFG
                     AKSNLHRVWDSQLLESSKYSYSEYSIYLQNKFEPQRATYQKFSLLQSVEAVYGLRNQI
                     YAYDLPTETNNYKYIYLFSDKLDEMLYLGGIQLANVLNGIYSK"
     gene            complement(25663..26439)
                     /locus_tag="PLZ54_00375"
     CDS             complement(25663..26439)
                     /locus_tag="PLZ54_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOJ65427.1"
                     /translation="MKKVLIIILLFGVQISFFVVHAQKNGLIGKSVIPNSKSIFFAAA
                     GPAYCFGDVGGSHYEQFLNGINDWDVLNTRYLFSVGYGQSFKHNLGYKLIFNYGNFIG
                     NDKNSRNEARQYSFNSEVSSLSLQAEYTFLGGIHSNYYDYYSLYLFGGIGVMNSNAEV
                     KKNGVAISQPPPDRIYDKIKLNETAAFIPFGIGYKYAITPKFSIGAELCWNYTFTDFI
                     DGISTVTSKENDLLANLSITFSYKFFDSLTPACRCLPYNQ"
     assembly_gap    26487..26586
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(26613..28274)
                     /locus_tag="PLZ54_00380"
     CDS             complement(26613..28274)
                     /locus_tag="PLZ54_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOJ65428.1"
                     /translation="MGKTVTIYCKNTRSYHDFPLGTSLIEIYRSLNIQLKYQVVAARV
                     NYKVEDLNFLIYKPKDIEFIDLSTSSGMRVYVRTLTMVVAKAVSELFPKGNIRIEHPL
                     SKGYYCVLNNIDEPLTQSLVDKIKERINLIISQGKTIICEEKQTSEVIKLFADQKDKI
                     VLFQTLGNPYCRYFRIDDYIDYYNGVLLPSTDYLNLYDLIPYYDGLLLRVPNRENPTE
                     LEEIVPQPKLYDIFKEYVEWNKIMNLSNVGEFNVACRNKQAFSLIKISEALHEKKVAT
                     IADTIAQRKDKVKFILISGPSSSGKTTFSKRLSVQLAVCRIKPVAISLDNYFVDRDKT
                     PRDENGELDFEHLHALDLELLNQQLRELIEGKEIELPSFNFEEGRRYYRGDKLKLAED
                     SLVILEGIHALNPKLLPEIPAEATFKIYVSALTTISIDNHNWIPTTDTRLLRRIIRDS
                     RYRNYSARETIARWESVRKGEEKWIFPFQENADVMFNSALLFEFAVLKRYAEPLLAEV
                     PKYCDEYTEAHRLIKFLNYFVPIPDREIPPTSLLREFVGGSSFRY"
     gene            28405..30204
                     /locus_tag="PLZ54_00385"
     CDS             28405..30204
                     /locus_tag="PLZ54_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOJ65429.1"
                     /translation="MHYTFYDLLTLIGSLGLFLYGMKTMSEGLQKATGDTIRSIMAAM
                     TRNRFTGILTGLLITSLIQSSSATTVMVVSFVNAGLLSLYQAITVIMGANIGTTITAW
                     MISIFGFKVSIAAFSIPLIGISIPLIFSSKSRNKSIGEFLIGFALLFMGLELLKNSVP
                     DIQGNPEVLNFLAKYTNFGFGSILLFLLIGTILTIIVQSSSATMAITLIMCSKGWISY
                     ELGAAMVLGENIGTTITANLAAIPTNVSAKRAALSHLIFNIFGVIWMLIAFRPFINLV
                     TMLITQFGPGDPTQLTAFSNSLDPDTLKVISSGSENLSPEQIAVRNQLENYQIATSYG
                     LSLFHTLFNITNTLVLIWFAKIIEKISTLLIKKKDTDEEFQLQFISTGLLSTSELSLI
                     QAWKEIKVYAERSHRMFSLVRELLQEKNENDFIKKFSRVQKYENISDRMEVEIAHYLT
                     KVAEGKLSEKGKFQIQTMLKVISEIESIGDSCYNLARTIKRKKDDKAEYTKEMDDNLN
                     EMMNLVEDALLQMRAAIDNNHVSKEELNRAENLENEINNFRNQLKNKNIDDVKENKYD
                     YQASVTYMDMLEECEKLGDYIVNIVEALYEASK"
     gene            30426..31361
                     /locus_tag="PLZ54_00390"
     CDS             30426..31361
                     /locus_tag="PLZ54_00390"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HOJ65430.1"
                     /translation="MDYILIISGFAVLVLGANLLVDGASSLAKKLKISDLIIGLTVVA
                     FGTSAPELVVNVIASVKPNATDIAISNVIGSNIVNIFIILGFSALFHPIKSEKTTTLF
                     DIPLSFFAIVVTFLLAFTGDRILSRTDGLVMLGFFIAFIYFTTRRSIKHPLIPLEENI
                     KIRKTGISILMISCGLAALIGGAEIIVPSAIRIAENLGISQAVIGLTIVALGTSLPEL
                     ATSVVAAIKKNSDIALGNIIGSNIFNIFLILGVSSVIRPLPIYRGIGIDLTMGALGSV
                     MVFVFVFGSKSHSIKKWQGLLLIFIYLMYAIFKIF"
     gene            31402..32526
                     /locus_tag="PLZ54_00395"
     CDS             31402..32526
                     /locus_tag="PLZ54_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ65431.1"
                     /translation="MNLFDVYPLFDIEIVKGQGCRIYDKNDTEYLDFYGGHAVISIGH
                     SHPYFVKKLADQIQKLVFYSNSVKNSLQVELAEKLGKIAGYDDYSLFLINSGAEANEN
                     ALKLASFHNGRSKVIAFGKSFHGRTSAAVGVTDNPAIQAPINRNFEVEFLPLNDTEAV
                     LQSLKNRDVCAVIIEGIQGVGGIQIPQPAFLEAISAACKETETVLILDEIQSGYGRSG
                     KFFAHQYADIRPDIITTAKGMGNGVPIGGVIISPIFKPTYGMLGTTFGGNHLACTAAI
                     AVLDVIEQEHLMENALQVGNYLINELQKFSRLKEVRGKGLMIGIEFDEPVKKLRERLL
                     FEKKIFTGVSGSNIIRLLPPLCLTMQDAELFLDRFAEVIK"
     gene            32549..>33162
                     /locus_tag="PLZ54_00400"
     CDS             32549..>33162
                     /locus_tag="PLZ54_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65432.1"
                     /translation="MKKPIALLFILFFVNSWTVFSQSVSKERDKVVMKTGEIYYGEVV
                     FRNDEMLILQTDEGVRYQFSIAEVKAIYKETVDGSDELEKMQQEGQSDEIQNFFAVLD
                     ACGGISSAKNSFDVSSNTQVAFVLGKDKVFSENFFVGLGVGYNSSYLKSNSSLNFLPV
                     FLRLQNTFTNNRISPFVRMDAGYAFSMTSGWDGGALVKAAAGISS"
     assembly_gap    33163..33262
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33263..33474
                     /locus_tag="PLZ54_00405"
     CDS             <33263..33474
                     /locus_tag="PLZ54_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65433.1"
                     /translation="TSGWDGGALVKAAAGISSRLSYKTKLFCGVYAGIQQFGGKLSET
                     ISSGTYYYEGTTNMKQLGLQLGIQF"
     gene            33521..34234
                     /locus_tag="PLZ54_00410"
     CDS             33521..34234
                     /locus_tag="PLZ54_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOJ65434.1"
                     /translation="MSKINLSQVIIAALNEQACAEKAQHHLRFFKTAPGEYGEGDKFI
                     GVNVPDQRKIAKKYFREINLNELEVLLTNEIHECRSTALFMLCYKFDKAGEAERTLMA
                     DLYLNNLSFVNNWDLVDASAANILGCYLMDKKRDLLYDFVQSNDLWKQRIAIIATFYF
                     IRCGEFEDTFKISEMLLHHPHDLIHKAVGWMLREVGNRDKEAEINFLLKHYRQMPRTM
                     LRYAIEKFDEGLRRQFLKG"
     gene            34464..36923
                     /locus_tag="PLZ54_00415"
     CDS             34464..36923
                     /locus_tag="PLZ54_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOJ65435.1"
                     /translation="MKLKEIAQILGNSHLKVPEVEINWLLTDSRALSFPTRSLFFALR
                     TRRNDGHRYIQELYDQHLRYFVVSETLPEFEKMHDAIFIRVNDTLEALQHLAAVHRNA
                     FQIPIVGITGSNGKTIVKEWLYQLLHSDFNITRSPRSYNSQIGVPLSVWGLNNNTELG
                     IFEAGISQPHEMERLEPIIRPTIGIFTNLGDAHQENFSSLKQKCEEKLKLFAHAQVLI
                     YCSDNRLVDITIAQSNFKGKLFSWGRNENAVLQLVKTKKLSDSTEVVYKFSGKESAVS
                     IPFSDDASIENAMHCVALMLHLGIDLEKIRQRIAKLESVEMRLELKEGIHGCLLINDS
                     YNSDLNSLAIALDFLNQQAATKNLPRTLILSDILESGKQSDDLYNAVAQLVKSKSIQR
                     FVGIGPEIVKHADKFEVEQQNFFEFTEDFLKSPLIHELQKEIILLKGSRKFHFEKISE
                     KLELIAYETTLEVNLNALINNFNYFRSKLRPETKVMSMVKAFAYGSGSVEVARTLQLH
                     HCDYLAVAVADEGAELRREGIRIPIVVMNPEKGSFGMIFEHQLEPEIYSFRILESFLS
                     AAEKLGVTDYPVHIKIDSGMHRLGFEPHEIVPLIQRLKAQNNLKVRSVFSHLAGADEK
                     RFDDFTRQQVKVFTECADLFAQSFEHPIIRHILNSAGIERFPEFQFDMVRLGIGHYGV
                     SALPKVKLEPVCALKSIILQIKNVKKGETVGYSRKGIANEDKRIAVIPVGYADGFDRK
                     LGNGVGEVLINGKRAKVMGNVCMDLTMVDITGIEAKEGDSVEIFGNNLSVIEVADKLK
                     TIPYEVLTSVSKRVKRVYFQE"
     gene            36981..37751
                     /locus_tag="PLZ54_00420"
     CDS             36981..37751
                     /locus_tag="PLZ54_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOJ65436.1"
                     /translation="MILIFVQNTTPPVALLQSVAILSVQLKKEWGILCLTENADLVSV
                     YENQYGEFLKTNGFPEVSIFVRQACCEQLVEFCEQLEASFLFFQLHDASHRYLRRSLN
                     FCRDLRIPYLFFKNSFSVLNLEKVVVPIGFLEEEIEKAQFASAFGRFSNAEIILLPAN
                     DYGSKAATTTDKMKTILDKFQLPYTVEKAQSDSFKIDREAIEKAVEMGGGLVIISASR
                     DYGLDDILFGPKELHLIRKSPVPLLLVNPRSDLYALCD"
     gene            complement(37768..38418)
                     /locus_tag="PLZ54_00425"
     CDS             complement(37768..38418)
                     /locus_tag="PLZ54_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HOJ65437.1"
                     /translation="MKSLHIGYIPDLDKADITTASEILEKNGTKAAIESQNWAKEFPY
                     KPIAYFHIAQSKEAVFIKFFVLCNALRAVYTHDQDPVYEDSCVEFFCKTIESEKYINF
                     EFNCIGTCSASRRKGRNDEVEPLSETEMKSIERFPSIVPQPFEEIKGNFDWELTVKIP
                     FKLIGIDPNNLPEKILGNFYKCADGTESPHYLSWNPIKTEKPDFHRPEFFGELFLK"
     gene            38850..40169
                     /locus_tag="PLZ54_00430"
     CDS             38850..40169
                     /locus_tag="PLZ54_00430"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOJ65438.1"
                     /translation="MKVLKFGGTSVGSAQRMKNVAKLICNGEKKIVVLSAMSGTTNSL
                     LEIADYFYKSNLDGALEHIITLENKYSDVVEELLTTEEKRQEARSILSSHFDYLRSFA
                     KSVFTIFEEKAIVAQGELLSTALFQLYLEEIGENSILLPALEFMRIDKNAEPDNAYIK
                     QHLNDLLEAHPGHEIYLTQGFICRNAYGEIDNLQRGGSDYTASLIGAAVNAEEIQIWT
                     DIDGMHNNDPRFVESTKPIPMLHFEEAAELAYFGAKILHPTCILPAKLANIPVRLLNT
                     MEPEAAGTLISTRMNKGKIKAVAAKDGITAIKIQSGRMLLAYGFLRKVFEIFESYKTP
                     IDMITTSEVGVSVTIDNTKKLDEIVNDLKKLGTVTVDHDMVIVCVVGDLPFENVGFQA
                     KVVDALKDIPLRMISYGGSNYNISFLIKAEDKKRALNALSQALFSEE"
     gene            40219..41382
                     /gene="lysA"
                     /locus_tag="PLZ54_00435"
     CDS             40219..41382
                     /gene="lysA"
                     /locus_tag="PLZ54_00435"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOJ65439.1"
                     /translation="MKAKYPVEKFLKLQTPFYYYDMDILRQTLQEVNRQIQKRNYVVH
                     YAMKANANPVLLHEIKNAGLGADCVSGGEINAAIKAGFPKEKIVFAGVGKADWEINLA
                     LDENIFCFNVESIPEMEVINQLAAAKSKTASIALRINPNVNAHTHQYITTGLNENKFG
                     INLSDLDTVLDLLPQLKNLKLIGIHAHIGSQITDMTSFQSLCVRINEIQNYFFQRHII
                     LEHINVGGGLGINYEHPNHFPIPDFESYFNVFAEHLNLQPYQTLHFELGRAIVAHCGS
                     LISRVLYVKQGATKKFVILDAGFTDLIRPALYQAYHRIENLTSDLPEEEYDVVGPICE
                     SSDTFAKNYSLNGTRRGDLVALRSAGAYGEIMASQYNLRSLPKAYTSDEIAAL"
     gene            41488..42651
                     /locus_tag="PLZ54_00440"
     CDS             41488..42651
                     /locus_tag="PLZ54_00440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ65440.1"
                     /translation="MTEIFGFSLSLLTLVLLCGLFLVFCYQIYFYCRYINGVIRYRNK
                     IRKGKVAFLDEKPGVSVIICSKNEANKLKEFLPSILEQDYPDFEVIVVNDGSTDETDM
                     LLIDLLKKYPRLRTTFVPMEATNLSTKKLGISLGIKAAKNDFLLFTDADCQPKSKDWI
                     SLMARNFVPKVEFVLGYGAYFHRKGLLNRLINYDTFFIALQYMGMAIAGKPYMGVGRN
                     LAYRKTTFLHHNGFSEILGLISGDDDLFVNKTANGNNTRVEISPESITFSEPKTTWKS
                     WYLQKARHLSVSPFYTWQSKLMLFMEPFTRGLFYVFCLVLLLQGDMIISLVVAAIFLI
                     RYFTQLYIVNRTAKHFNERRFFLLLPIFDLVLPILSLSIFITGKASRRKNKKW"
     gene            complement(42673..43206)
                     /locus_tag="PLZ54_00445"
     CDS             complement(42673..43206)
                     /locus_tag="PLZ54_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOJ65441.1"
                     /translation="MRIISGRLKGRNIYAPVDISARPTTDFAKEGLFDVLNNRIDFED
                     IEVLDLFAGTGSIGFEFVSRGCRQVVSIENNPKQCLFIKKISEKFEIDNLILLKTDVF
                     KYITHTASRFDLIFADPPYQLENLKEIPDMIFSQQLLKTEGIFILEHSSKYNFSQHPH
                     FMETRKYGRVHFTFFKA"
     gene            complement(43217..43960)
                     /locus_tag="PLZ54_00450"
     CDS             complement(43217..43960)
                     /locus_tag="PLZ54_00450"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOJ65442.1"
                     /translation="MYSSRGDLESTNTYIQPYSLSIRFSPGGLSLSILERDNTKLVSA
                     KRLNMPLFSMTKEQIIPLLLQEPETQLLYDKIHLIAETKTYTIVPKEIFKEEEMDKLL
                     YFSHQKEKNASILYNEISAFDAINIFSLPEDISEVFKQLFPACKIEHSISHFIENHIH
                     KHSLQECICISVRQKIMDVVAFQHNRLLLVNSFDYQTQEDFVYYLLRIIEELNLDLQN
                     CKVYLYNMEQHSKLQGFVRKFVKQCETID"
     gene            complement(43993..44673)
                     /locus_tag="PLZ54_00455"
     CDS             complement(43993..44673)
                     /locus_tag="PLZ54_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65443.1"
                     /translation="MKTVIKILLSLSIVLLSYLCVASIITPIRFERIKNQREKDVIQC
                     LVDIRKAQIEFKDQNKRYTASFDTLFHFLKTAKKKIVMKEGFLTDKQLEAGLTEAKAA
                     EIVRKGNQKEIAEYGLQNFRRDTILTDLLEEVFPNYNRDNIDKMRIIPYSKNEEFTLK
                     VNNNYTNISGIRIPLFEASALYESYLYDLNRQELLNLIDRTLKLGKFPGLKVGSIEEP
                     NNNAGNWE"
     gene            45154..45972
                     /locus_tag="PLZ54_00460"
     CDS             45154..45972
                     /locus_tag="PLZ54_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65444.1"
                     /translation="MKKMLCLLLIASTVSQIFPQISSIIPVSSSIAGTSVANTGLRSE
                     FQNPAMLGYAKNSEIGMQHENRYALSELSTNSFQALLSTSAINADLSFSYFGYSLYHE
                     MMAGLGFGRNFSDKFALGVRFNYYMAYFSASNSYRGALLPQIGLSYRFSSLFSLGFST
                     FNPFQTNIKTDFTQKRIPSIFSLGTEYYFSPDLVWLAQIDREISSNYRLATGFEYQML
                     QQVSLKLGAYDAGYLVPCGGCGLKWNRFLLDINCELHPLLGLNSYAGLRYIFSR"
     gene            45996..48068
                     /locus_tag="PLZ54_00465"
     CDS             45996..48068
                     /locus_tag="PLZ54_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ65445.1"
                     /translation="MKRIVILVCCLLFLLLNAAYAQDASTTIDQTIADIFEQWSAESE
                     EVPDYQTFYEELEFLAENPINLNHTSREELSKMLFLSDVQVENILAYLYQYGPMENIY
                     ELQLIDGLDMTDIRRMLPFVMVKKEKAVSEKIYRYDVLNYGKNELYMMFNRDAETRQG
                     YQASFSSIGDSTNQGKKYAGSPLYHSAKYRFHFKDRILAGMTFEKDAGESFFGSAHKG
                     YDSYSGYLQINRLGIFKTIVLGDFRANFGQGLVMKSDFSMGKSSYVLNVVSRNEGLKK
                     FSSTDEYNFFRGGGATIRLPRNWETSVFYSNKMIDADTVGGVFSSIYKTGYHRTENEL
                     NKKHTICQQVAGGNISWRLLWLQTGITFVHTNFDQVLVPEKTTYNRFYFSGKAQSTAG
                     WNYRVHWQKLNLFGEAAVTGNFHPALLSGFTLSPLSLVNLVAVYRYYSPEYDTFYALA
                     LSETSRTNNESGLYLGAEINPAKKWKISAYADSYRFPWPKYGIDAPSAGKDYLFQTDF
                     SAHRRLSMFWRFRHSNKPANISQENSVMPVVKAVKKNSLRYQLMYSQGNFSFRTAAEG
                     NMVQKGENADYTYGLTAYQDLTYEFSSLPLKLDFRYQFFDAVAYENRLYAYEKDVLYA
                     FSIPMYYGIGSRYYLNLKYELNRQFTLWFKIAQTVYADDREEISSGNEKILGNKITDV
                     KFLLRFNF"
     gene            complement(48302..48724)
                     /gene="tsaE"
                     /locus_tag="PLZ54_00470"
     CDS             complement(48302..48724)
                     /gene="tsaE"
                     /locus_tag="PLZ54_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOJ65446.1"
                     /translation="MTEIFDLNNIQQPARKFINLFDRYSVFAFYGSMGAGKTTFIKAV
                     CEILGVKDTVNSPTFSIINEYEAADGRVLYHFDCYRINKLQEAIDLGAEDYFYSGKIC
                     FIEWPEKIEALLPDSTVKVNIQEVENGIREIEMILPTI"
     gene            complement(48782..50620)
                     /gene="uvrC"
                     /locus_tag="PLZ54_00475"
     CDS             complement(48782..50620)
                     /gene="uvrC"
                     /locus_tag="PLZ54_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOJ65447.1"
                     /translation="MNLHPQLKQQIDALPEKPGVYQYFNEKEEIIYVGKAKNLKKRVS
                     SYFTKTHESRKTAVLVRNISSLKYIVVDTEEDALLLENNLIKKFQPRYNVLLKDGKTY
                     PSICITNEEFPRVFKTRNIIKNGSLYFGPYSSSYTINSLLEIIHDLYPIRTCKLQLTD
                     ENIKAGKFKVCLQYHIHKCNGPCEGKESAEIYRKYINEIKQIIEGNANEISKMLWEEM
                     QKLSAAYKFEEAYQIKQKYDLIENYKSKSIIANSVVENTDVFGYEETENAAYINILRI
                     SKGSIIQGYTIEYQKHVDEEKEDLLAMAIVELRERLKSNSKEIVVPFEINYKPNGVSI
                     VIPQRGDRKKLLDLAQQNVLQYKFEKLKQAEKLNPDQRSMQLLKEIQEKLHLEKLPIT
                     IECFDNSNIQGKDAVAACVVFKKGRPSKKDYRKYNIKTVVGPDDYASMKEVVKRRYSR
                     MIAENGELPDLIIADGGIGQMEVIREVVEDELNLRIPIIGLAKDSKHHTREILFGFPP
                     KNIGMTPNEPLFKLLATMQEEVHRFAIKFHREKRSKNQTTSELDNIKGIGDKTKRELI
                     NHFKSIKRLQNANLEDIQKVVGNHRASIVYGYFHKDLSAPESQISK"
     gene            complement(50701..52575)
                     /gene="mnmG"
                     /locus_tag="PLZ54_00480"
     CDS             complement(50701..52575)
                     /gene="mnmG"
                     /locus_tag="PLZ54_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOJ65448.1"
                     /translation="MDFKYDIIVIGGGHAGNEAACAAANMGSKTLLITMDMNKIGQMS
                     CNPAVGGIAKGQIVREIDALGGQMGVVTDRSAIQFRMLNRSKGPAMWSPRSQSDRHQF
                     ILQWIQTLTNTPNLYIWQDTVKQILIENGQVTGVLTALGIEISAKCVVLTAGTFLSGL
                     IHIGKNQLIGGRISEPASFGITEQLKSLGFTTGRMKTGTPARIDGRTIDFSVLKEQPG
                     ENDFHKFSFLTEVTRELKQMSCWITYTNELTHDILRQGLPESPLYNGQIKSIGPRYCP
                     SIETKIVTFADKSSHQLFLEPEGVDSQEYYLNGFSSSLPVQVQIQAIHTIPGLEKAEL
                     YRPGYAIEYDFFDPTQLKHTLETKRIKNLFFAGQVNGTTGYEEAAGQGLIAGINAHIN
                     CHGGKEFLLDRDEAYIGVLIDDLVTKGVDEPYRMFTSRAEYRILLRQDDADLRLTPKG
                     YEIGLVTKNRINQYSFKYEAIESLKTFVENFSIKPAQVNGFLVSKNSSELTHGCKLVD
                     LILRPQISIMELSEIVPALKSKIQSIGDRVEEIVEAAEISLKYKGYIDREKSIAEKFH
                     RLEHINIKGKFDYHTISSLSTEARQKLSKIDPETIGQASRIPGISPSDINILLVLMGR
                     "
     gene            complement(52656..53147)
                     /locus_tag="PLZ54_00485"
     CDS             complement(52656..53147)
                     /locus_tag="PLZ54_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOJ65449.1"
                     /translation="MSENFFISSSLDKKSKYEILLQQIPALIADEPDIVANLANLSAV
                     FHQTFKWWWVGFYLVKEDFLVLGPFQGPVACTRIAFGKGVCGTAWKEKRSLLVPDVND
                     FSGHIACSSISVSEIVIPLYNSSSEVVGVLDIDSEKFGVLDEADIYYLEQISTIIGKA
                     LFA"
     gene            53363..56185
                     /locus_tag="PLZ54_00490"
     CDS             53363..56185
                     /locus_tag="PLZ54_00490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOJ65450.1"
                     /translation="MRKLEFKFFPFVLLICLNTSMFAQQVQFQPLPIDPKVKYGKLEN
                     GLTYYIRSNQQPKNRADFYIVQNVGAILENDDQNGLAHFLEHMAFNGTKNFPGKKIIE
                     YFESVGVKFGANINAYTSLDETVYHLSNVPTNRESIIDSALLVLHDWSNAISLEESEI
                     EAERGVILEEWRQGASADRRMWKESNKQKFPGSQYAKRDVIGDTAVIKHFTPEALRDY
                     YKKWYRPDQQAIIVVGDVDVNKIEEKIIELFSNIKKPENAPERPIYPIYDNKEPIISI
                     VTDPEARMTRVELIYKHDKLPAEIKLSINGYLVNVINSLISNMMQNRFEEITMQPDAP
                     FVAGYATYGELVKSKDAFYLVVVPKEGSETEGIKSVLIEAEKVKRFGFNHSELERAKA
                     DLAKMIEKQYNEKDNQKNESYVREYMEHFLNQDPIPGIEWEYETIKMILPQINADMVN
                     QLAKSYVTDTNMIVSIMAPQKPTVVLPSKEEIRQIIEEVKKTELTAKLDEKIDKPLIE
                     KMPKPGKIKKVSKNDILGTTEWILSNGAKVILKPTTFKKDEILFNAYSIGGLSKVENI
                     EDLPSAEFATEVVSNNGLSDFNQIELKKLLSGKIASVSPYIDNYEEGFSGNSSVSDFE
                     TMIQLLYLHFTALRKDDNAFTALMNNYKAFLANSENNPDKAFSDSVKLMLSNHSPRTV
                     LLNMKTLEKVNQDKALEIFAERFSDPADFTFVFTGNVDPNDKTVVNAINTFIGGIKSK
                     GKKESFTDDNVRLPVGRANNHFSREMQVKKVSNCIVYSGNLNFNMNNRTNMNAIGNIL
                     NIRYLESVREKEGGSYGVGVAANLRNRPIEQAMLFIQFDTNIEKQNKLMDIIHSEIDT
                     IISKGPKAEDLQKVKETMMKKYEEDLQENKWWQSAIKNYYFNKLNVVNDYLPSVQALT
                     SESIQKTLKTIVEQGNIMEIVMKPKE"
     gene            complement(56278..58272)
                     /locus_tag="PLZ54_00495"
     CDS             complement(56278..58272)
                     /locus_tag="PLZ54_00495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HOJ65451.1"
                     /translation="MMLMMNGCKSEKKISGIDLTNLDTTARPGDDFYQFACGGWMKKN
                     PLKGEYARYGTFDKLAEENTSQLRELIEEIASKSHVEGTVEQKIGDLYQLAMDSDRLN
                     REGYQPVQTELKKLAGVKTNTELIKILPELLLNGNDVYFSVYVDADPMNSSVYLTQTY
                     QSGISLGERDYYLDQDTHSKEILEKYKQHVAKMFELFGFNSADAQKNRDAVLYIETRL
                     AKASYDRVKLRDPYANYHKMSIQDLSKEFSTISWNEFLHTLGIPAVDSVNVSQKEFLT
                     EVLNILTTVPLDQQIAYLQWKVIDNAAAYLSDSIYNQNFEFYGKTLSGKKEPSPRWKR
                     SVRSIDNFLGEAVGQMYVKKYFPPEAKESMLKLVHNLQESLGQRIQNLTWMGDSTKAK
                     ALEKLGTFYVKIGYPDKWRSYDALQISKNVSYYENVKRAHHFEYAYKFARAGNPVDKS
                     EWLMTPQTVNAYYNPSTNEICFPAGILQPPFFDVHADDAVNYGAIGVVIGHEMTHGFD
                     DQGCQFDKDGNLKNWWTEEDKKQFDARAKVLADYFDSIEVAPGVHANGKFTLGENIAD
                     HGGLQVSFQAYKNSTKGQPSKILDGFTPEQRFFLSYANVWACNIRPEEILVLTKSDPH
                     ALGRWRVNGALPHIQAWYDAFGIKEGDKLYLPVEKRACIW"
     gene            complement(58377..59399)
                     /gene="dusB"
                     /locus_tag="PLZ54_00500"
     CDS             complement(58377..59399)
                     /gene="dusB"
                     /locus_tag="PLZ54_00500"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOJ65452.1"
                     /translation="MKISNIEFPSRPLFLAPMEDVTDADFRLLCRHFGADMVYSEFVS
                     SEALNRNIPRTLQKINVLEEERPVAIQIYGTDPEVMAESAQKVEMIHPDIIDINFGCP
                     VKKVAGKGAGAGLLKDIPRMLKITQFVVKAVNVPVTVKTRLGWDDSSKIIVELAEMLQ
                     DCGIQALTIHGRTRQQMYKGEADWTLIGEVKNNSRIKIPIIGNGDITRPEQAKDFFNR
                     YGVDAIMIGRAAIGQPWIFSEMKHVLDDDFEYSPLSFEDKKAILKNYILSAVRRLDID
                     EQTPIENVYDIHLKGIVHSRRHLAATSIFKNIPNFKSTRIAMLRADTLRELFDIIDSV
                     HETFCN"
     gene            59626..60924
                     /locus_tag="PLZ54_00505"
     CDS             59626..60924
                     /locus_tag="PLZ54_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOJ65453.1"
                     /translation="MENYINKTETYPTERSKIEIMAPAGSWESLAAAIKAGADSVYFG
                     IGKLNMRSKSSSNFSVDDLKKIVEICTENNVKSYLTVNTILYDNDLISMREIIETAKE
                     AGISAIIASDVAAMMYANSLNVEVHLSTQLNISNIEALKFYAQYADVVVLARELNLKQ
                     VSEIFSAIQTKNICGRNGKLIRIEMFCHGALCMAVSGKCYLSLHEKNLSANRGECNQI
                     CRRGYLVKDKDSEIELEIDNEYIMSPKDLKTIEFVDEMLKAGVSVFKIEGRARGAEYV
                     KTVVSCYKEAINACYEGTFSKEKINDWNRRLSKVFNRGFWNGYYLGQRLGEWSKNYGS
                     AATHKKIYIGKVTNYFNKIGVAEFLLEAQSLSVGDEILITGETTGAYEDIVNEIRVDL
                     QPVEKVEKGTYFSMKTNELVRRNDKLFKIVPTEHGKQEGK"
     gene            complement(60957..61880)
                     /locus_tag="PLZ54_00510"
     CDS             complement(60957..61880)
                     /locus_tag="PLZ54_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HOJ65454.1"
                     /translation="MTQVTDKVNTSGSKNTQSNVVVDWFIRLIKGIIVGIGFILPGLS
                     GGVLAVILGIYDRIIKFLSDLRKDFVQNVFYFIPVVIGGAIGIVLFSILVEKAFGNYA
                     AQFICLFVGFVGGTFPSLYRTAGKQGRRTKDIIIFIFSTFFIFGLMLLGGQQLTEVAP
                     NIPVWLGSGALIGLGVIVPGMSPSNFLIYFGLYDKMATGIKDFDFAVIIPLLVGFAVC
                     VLLFAKLAAYLFRKFYPEMYHFILGMVVGSSLAIFPTVVFPAFRSEQLSVAGLSFVGA
                     FLLCIGFLAVGVVISFLFSKLEDKFPREEIF"
     gene            complement(62017..62829)
                     /locus_tag="PLZ54_00515"
     CDS             complement(62017..62829)
                     /locus_tag="PLZ54_00515"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOJ65455.1"
                     /translation="MILPIYFLPLAGLLIGILISLMGGGGGIFYIAILTGLFSIPMNQ
                     AVSVSLATIIPTTFAASISHFKAGNVNINVGLILSAGGIIGVIAGSFLLGLLSLNVLK
                     IVFGLFMIFMAVQMIFKNFRSKKSFNKGNIILVEEDKTVNIAKKKKIFSFKNFKGLSF
                     GFFGGIMSGLLGVSGTPPILAGLYNMKLSSVQVVGTSVMVLFFIALSGVITHTAFGHL
                     NWYLVGLLAAGTITGAILGPILGKKIDEKKLEKFYTPFFIVFIFLMGISMFF"
     gene            complement(62858..63787)
                     /gene="nadA"
                     /locus_tag="PLZ54_00520"
     CDS             complement(62858..63787)
                     /gene="nadA"
                     /locus_tag="PLZ54_00520"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOJ65456.1"
                     /translation="MEKKELIEKINKLRKDKNAVIMAHFYQEPDIQDIADHVGDSLAL
                     AQWAAQTDADIIVLCGVHFMGETAKILNPQKKVLIPDMNAGCSLADSCPADAFEEFVK
                     NHPDYTVISYVNTSAAVKALTDVVVTSTNAKKIVESFPKEEKIIFAPDKNLGNYINSI
                     TGRQMLLWNGACHVHEQFSVQKLVELKKKYPEALVLAHPECTKPLLMLADFIGSTQAL
                     LNFATSSDKKFFLVATESGILHEMQKKNPDKQFIPVPPNDSTCACNECNFMRLNTLEK
                     VYQCLLNESPEILVEEEIRQKAVKPILRMLELS"
     gene            64282..>64997
                     /locus_tag="PLZ54_00525"
     CDS             64282..>64997
                     /locus_tag="PLZ54_00525"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HOJ65457.1"
                     /translation="MKIFSTSVVRRLDEYTIKKEPIASIDLMERAAVGLCKKFLEIFP
                     SPLPVCIFAGPGNNGGDALALARMLLQKNYYVTVYLIHTGKLSADCEANKIRLIEKFP
                     GCLHDFSTDFYPVNFSENTVIIDGLFGTGLSRPLSGIYSEAVGWINNSGKTVVSIDIP
                     SGLEGEENKDLSQPIVKADYTFSLQFPKLSFFFAENESFVGKWEIIEIDIHPEAIRRE
                     PTDFYFLEKKDVIPFLPERK"
BASE COUNT        20771 a        12072 c        12046 g        19908 t
ORIGIN      
        1 ctgataaaat gagattgtgc caaatgttta atgattttca tcagaatata atttattttt
       61 taaaggtctg atggaaccca catgtctgaa tattatatat tcattcgtgt ttgtgtatgc
      121 aatacatgtg ggtttcttaa cttttattga cagaggaacc ctatttcgtt tttaaagttt
      181 atagattact ttccttgaaa gtccaaatta aaactcattg ataaaaactt ctcacaaaat
      241 ttacaaaaga gtgtttcttc atttttttgt aattttgcaa aaacaaaaac agacttccga
      301 tagttgtata aaacatgaat aaagaagaaa ttgaacatat cagggccgat tttccgattt
      361 taagcgagaa ggtttataac aaggatttga tttatttcga caatgctgcc accacacaga
      421 aaccccgttg cgttgtagaa aaaatcgaat atgcttattt tcacttgaat gccaatattc
      481 atcgtggtgt tcactatctg agtctgaaag ccacagaagc gcacgaagca gctcggctga
      541 ccgctgccga atttcttcat gcagaaaaaa aagaagaaat tatttttaca cgaggcacca
      601 cagaatccat caatctggta gccttttcat ttggtgaagc tttttgtcat cctggcgacg
      661 aagtgatcgt ttcggcaatg gaacatcatt ccaacattgt cccttggcaa atgctttgcg
      721 agcgcaaagg aatgaaactg cgcgtaattc ctgtcaacga aaacggagaa ctcgacatgg
      781 ttgcctattc ttctctgttg aacgaaaaaa cgcgtatcgt atcggtggct catgtttcca
      841 atgtattggg aaccattaat ccggtgaagg aaattattcg tattgctcac gaaaaaaaca
      901 ttcccgtttt gattgatgga gcgcaggctg ttccacatct ttcagttgac gtacgggaat
      961 tggatgccga tttttatgtt ttttcgggac ataaaattta tggccctacc ggcatcggca
     1021 ttctttacgg caaggaaaaa tggttgaatg ccattccgcc ttatcaaggt ggcggagaaa
     1081 tgatttctac cgttaccttt gaaaaaacca cttacaatga acttccattc aagtttgaag
     1141 ccggaacacc cgattatgtc ggctcaacag ctttggccga agcgcttcga tatattaaca
     1201 acatcggact cgacaacatt gccgcttatg aaaatgaact gctccattat gccgaacagc
     1261 agctgatgga aattgaaaat atgcacatta tcggcacagc aaaaaacaaa tgttccgttc
     1321 tttcttttct ggtcgatgga atacatccct acgacattgg catgttgctc gacaagctgg
     1381 gaatagccgt tcgaaccggt catcactgcg ctcaacccat tatggatatg tttggaattc
     1441 ccggcaccgt gcgtgcttca tttgcttttt acaataccaa agaagaaatc gtacgcttta
     1501 cggaagccct caaaaaagtg gtaaatatgc tgaaataaat gttgcagaaa aaaatacaat
     1561 ttcatgaaaa tttttccaaa aaaacattaa attatcagaa agaaaatgac aatcaacgaa
     1621 ttacaggatg aaataataga acagttcagt cagtttgacg actggatgga taaatatgct
     1681 ttgcttatcg atatgggaaa ttcgctggaa ccactcgaaa atgaatataa aacgcctcaa
     1741 aatctaattg aaggctgtca aagtagggtg tggctggatg cacaaatgaa ggatggaaaa
     1801 atctattttc gaggcgatag cgatgcggta cttgtaaagg gaattgttta tctgctcatc
     1861 catgttttgt cgggacacac gccggacgaa attttgaatg ccgatttgta ttgcatcgat
     1921 aaaataggct tgcgcgaaca tttgtcgcca acacgttcca atggtttgtt gtccatgctg
     1981 aaacaaatga aaatgtatgc gttggctttc aagtcgaaac aggaatgaat cggattattc
     2041 tccttgcggt tcaaaatatt cgagtcgaaa attttctcca tcgaaaacgc cataagaaaa
     2101 aattccgaca aaatcgccaa gaataattac ccttttttgt tgttgcaact gcaaatcgag
     2161 tgcgatatgt cgatgtccga aaaccaaaaa atccacctca tgattcaaaa tataattttt
     2221 ggcaaaaatc acaagatttt cattttcttc tcctaaatat ttattatcca ggctgagaac
     2281 tttctccctg ctttttttcg accaccaata accaaatttc tgccccagtc gcggcggcac
     2341 gagctgaaac aatcgctgag caaaggaatt gtgaaaaatt tttctgataa aaagaaattt
     2401 aggttcattt gtgtacaaac catctccgtg agccagataa aaaagtttgt ttcccaattt
     2461 caccgtcaaa ggctcacgat gcactttcat cccaatttcc tcctccaaat acccaaatgt
     2521 ccaaatatcg tgattgccgg taaaaaaatg aatttcaatt ccctgatcgc tcaattcggc
     2581 cagtttgccc aaaaagcgaa caaacccttt tggaacgacc gttttgtatt caaaccaaaa
     2641 gtcaaaaata tcacccaaca ggtaaatggc ttctgcatcc cgtcgcacct gctccaacca
     2701 acgaacaagc ttctgttcgt gctcgcgggg attttccacc aaacgtgaac ccaaatgagc
     2761 atcggaaaga aaataaatca tactttcttc cctcctctcg ctgcagtagt tttacgattg
     2821 aaatttatat gtgaaatatt tctcaaaata tatttttttt acagtaatcc aagttccagc
     2881 tttgcttctt cgctcatcat gcttttgtcc cacgccggct cgtacacaat gtttacatcc
     2941 acatctttta tgccttcaat actttccact ttcatacgga catcctcaat aataaaatcg
     3001 gcaacgggac aattgggtgc cgtcaaagtc atatcaattt ctacatgtcc gtcttcgtca
     3061 acatcgatgt tgtacaccag acccaagtca taaatattga cagggatctc cgggtcgtaa
     3121 accgttcgga gcatttgaac tatcttttct tccgtttcca aaaaatcact catgttaatg
     3181 gttgttgttt gttccatcaa taaaaaaatc ctacagcttt tcaaacactt cgagaagtca
     3241 aattgttgtc ggcacaagaa aaatttttta ctcttcgcct tttacggcat ggttgtcggc
     3301 caaaagtttt tcaaaaggtt cgaaagccga agccaatttt ccgttgatca ctactactgt
     3361 atcaactttt gctttggcgc aaagtgttcc ctctgccttt ttaaataaag cctgataaaa
     3421 actgtatttt actccttcct ttctcagata aagtttggaa agaaatactt cgcccggcaa
     3481 taaaggtttt ttaaatgcaa tttcgatgcg ggcaactact gcatcgatgc cttgctgatg
     3541 caattccgaa aaactgagat gattcgcctg caaaaattcg tgccgcgtat gttcaaagta
     3601 attttgataa accgcattat ttacaattcc ctgcaagtcg cactcgtggt cgcgcacctt
     3661 catttccagt tcataagtat aagtcatggc agttattttt ttctgattat cgttaaccca
     3721 tctcttaagg gcaatataac tttttctatt ctatcatcgg atgcgattaa atcgttaaac
     3781 cgcaaaattt ccatcgtttg tctgtcgttg ggttttgcct ccgtaacgac ttttccattc
     3841 cacagggtgt tgtcggccaa aataaaaccg ccatttctga gttttggaaa aaccgcttca
     3901 aaataagctg tgtattctcg tttgtcggca tcaataaata ccaaatcaaa aacttcattc
     3961 agagtcggaa taatttccag tgcattccct atatgcaact taattctgtg tccatgttcc
     4021 gaaccggcga agttttccaa aatcaaatct tccagctcat catcaatttc tatcgtatgc
     4081 aaaacatcct catccgtcaa tgcttctgca atgcacaaag ccgaatatcc ggtaaaagta
     4141 cctatttcaa gcgctttatg gggttgaatc attttgcaca acatggacaa tactctgcct
     4201 tgcaaatgtc ccgaaaccat tctgggattc agaagcttga catgtgtatc acgactgatt
     4261 tttgtcaaat aggcaggttc actgtccgaa tgttgcaata tgtaatcttc cagcgacatg
     4321 gatgcttaat ctttaaaaaa tgaaattcgg ttacaaatca accaaatgta cccttctcct
     4381 ttaagaaata ttttctttct gatggtgcaa aattatccat tttttgtcat atttcattga
     4441 ttcgaaatgt caagctctgt tttttataaa caatgttcag ggaaaaacta aaccctgttt
     4501 ataaaatgac tataaatagg gttgagttta tctttattta cttgtaataa cgatttgttc
     4561 atccggcaaa ccatctaccg ttgcttttag tgtaattttc cctttttttt cggtgctttg
     4621 aacaatggcc atacattttc cgaagaacaa atgacgttga ggttttttta atgaaattga
     4681 gtcgttctca ttgccgttat cggtgccggc aatcatgccc ggtcctttta gcgtaaattt
     4741 caccaattga tcagccagtg gatgaacatt attatctttg tcaagcagct ctactgtaac
     4801 aaatgacaaa tctttactat tggaagagat agtgcttcta tcggctgtca ggcggatttt
     4861 taccggggct ccggctgttt tgacttcata cgccagcact tcttttccat ccttgcgtga
     4921 aatagctttc aacgttccgg gttcatattt taccctccac ataacgtgca aatcgtctcc
     4981 ctttttgtcc ctctttccta aagaacgtcc attaagataa agttctactt cgtcggcatt
     5041 attgtaataa gcccacacgt ctatcgtttg acctttttgc caattccagt gtgggaaaac
     5101 gtgtaaaaca tctttgtctg tccattcact ttgatacatg taataaatat cttttgggaa
     5161 gccggccaaa tcgataattc caaaatagga acttcttgat ggccaggagt aaggagtcgg
     5221 ttcccccaaa tagtcaaaac ccgtccaaat aaattggcca gaaatgaaat cgtgtttttt
     5281 aactattttc catgtttttt cgtgggtaga gccccaggga acatgagaat tgtcataggc
     5341 agaacatttt tgcaaaggat tgaaaaaagg tttatcccaa ctttcgggcc aaatatatat
     5401 gctgtcggat ggcataaggt aaaatcctct tgtttgtact gccgatacag attctgtaac
     5461 aataaagggt ttgccaggaa agtttttagg aacatccata aattcgttgt gatgataatt
     5521 gaaaccaata atatcaagtc ctccagacaa aaaaagcgga ttgttttttt gggttccatt
     5581 gcaagctgct gttaccggcc ttgtaggatc cagtaactta acaatatcgg aaagatgttt
     5641 ggttagcaag gaactttctg aaattttagc ttttggggaa tcggatattt ttaaattgtt
     5701 tagcaaaaga ttcgcttgat caagattcat gttgtcggtc gtaatgttgt cttcccattg
     5761 ttccatcact tcatttccaa tactccacat aaaaacggaa ggatgattgc gatctctttt
     5821 tatccaatcg gtcatatctg ttttatacca tttcggaaag tcttgagcat aatcataagc
     5881 cgatttcttt ttgaaccaca tgtcaaaaat ttcatccatt acaatgaatc ccatactgtc
     5941 gcaaagctgc aacagttcgg gagcaggagg attatgtgag catctaatag cattgcaacc
     6001 catgtcttta agcagttgca attgacgttc taaagccctt ttgttgatgg cagctcccaa
     6061 agcgcctaag tcgtgatgat tacaaactcc gttaattttt aaatgttttc catttaatga
     6121 aaaacccttt tgagcatcaa aattaaaata tcgaaatccg acggatgttt catattcatc
     6181 tgtctccttg ccttcaactg aaagttttgt aatcattttg tacaaataag gatgttcaat
     6241 atcccagaga atcggtttat tgaccgaaaa tgattgagaa gccacagcca gcgaatcggg
     6301 taaaacttct atcgtggttt cgttttttgt aattttccgc ccttcattgt ctaacaacac
     6361 ttgttcaact tttacaagcc gtcgttcgcc cgttgaattt cttactgaag tttgaatttg
     6421 aatagtcgct gcattttcgt tcacaacggg agttgtaaaa taagttcccc acaaatccac
     6481 atgaatgggt tgtgttatgg tcagccaaac attacgatat atgcctgatc ctgaatacca
     6541 gcgcgaattg ggctgcttgg aattgtcaac gcgtacggct aaggtatttt tttctccaaa
     6601 ttttaagaaa ggtgttaatt catagcggaa cgagatgtat ccataaggtc gtttgccgag
     6661 ataatgacca ttgatccaaa cttcactatt gcgataaaca ccgtcgaaat cgacatatac
     6721 gcatttattt tgctcggact gaggtacaaa gaaagttttt ctataccagc caatgccacc
     6781 gggaagagct cctccaccgg ctgtggcggg gaacttttca gaaaaatcgg attcaatact
     6841 ccaatcgtgg ggtaaattaa gagttctcca tgcaccatca tcgaatgccg ggcttgaagc
     6901 atccattgcg gaatcgctta aggtgaattt ccagtcttgg gtaaagtcga tgacttcacg
     6961 gagtgatact tgtcggctgc atgaacttgc aagaatacag gtcaacaata aataatgaat
     7021 aattttcata actatagatt tttttaaggt taacaataat aaattctgat tttttctgat
     7081 aaactggtgt attttttttt aatttaactg ctcataatta gaacattaat tttaactgct
     7141 ataaaacgtg ataaattaaa attcggatgc ccggccaaat ttttttcgta atcattatca
     7201 ttgcccatgt tccaatgttt gaaattttgc aattcaaata tacaacaata gggtgaatgc
     7261 ttaagcattt gtcccattca ttttacattt ttcttaaaaa tgtttatcgg tgaggagtaa
     7321 tattttgtta caatataatt cttaatttac taattattaa cttattaatc aattatttgt
     7381 tgaatcattg gttgtgaaaa aatattttat attacggatt ttttatatct ttgacaaatt
     7441 ttttcgatca aatccttttc gacagaatat gctaattaaa atttatgaga ccaatcccaa
     7501 tccccacgaa atacatcgtg taattgaaac tttacgcgac ggaggaacca ttatatttcc
     7561 taccgataca gtttatgcat tcggatgcag tttgttcaac aaacacggca tagaatatat
     7621 tgcacgcatc aagaaaaaag atcttcgcag ctcccaactt tcatttgttt gtgaatccat
     7681 aaaacaaata agcgaatata ccaaacttga tgacgaaacc tttaaactga taaaaaaaaa
     7741 tctgcccggc ccgtttactt tcattctcaa agggaatcat cgcctgccta aactttttaa
     7801 agataaaaaa acggttggaa tacgaatgcc aaaaaataat attgcactca ccattgttca
     7861 tgaattagga aatccattaa tgaccagttc tattttttac cacaacaatt cactggaata
     7921 tattgttgac cctgaaatga ttgaagaaaa atatggccat ttggtcgatt tggtaattga
     7981 tggaggaccc ggaggttata ttccatctac cattgttgat tgcacctgcg aagaaccggt
     8041 tattttgagg caaggcgctg gagaactgat ttggtaagta tttttttatg ttgcaaatca
     8101 ataacacaaa aacttaaaga aaacgaacaa gagggtatta tacaattatt tttaactctt
     8161 cgaaacttat cgaaagcatt tttgcataag cattgatgat catgatttgt acgatcagca
     8221 ttttacttcg gaaagtattt tttaagttac ctcccctaaa gcttattaat ttaaagaaac
     8281 aaagaaaatt ttctggcaca aacaaaaact tgtaagacgc taattttaac ccccattaca
     8341 cattaaccaa tttaaaggga aagccaaagt cagaagagct gttccataat caggaaaacc
     8401 aagctctgct tatttaaaac aattttgaga accagtttac tgttgatttt ttcaagatat
     8461 gcatcaactg cagaagtttt ttgtaaaagt tctatatctt agataaagtt ttctctgttt
     8521 ttatacaaga tttacacctt ttttcctgtt gcgggatgca gcgataaaaa ggaaactact
     8581 ctgataaaac ttcttttttg ttttaagaca aagaaatttc aggatggact aaactttaaa
     8641 tttttttaat gacggaagat attgcaagac aacttttgct aatgtcgata aatttcagtc
     8701 gatgttaaac gaaacctcaa tgacatgata attaaaatac gggaaaaaca agcaattaaa
     8761 acttaacttt caaaaaccaa tcatttttac tacttttgca aattattttt ttaaaactca
     8821 tcgttaaatc atccaaaagc aactgttggg aaatggagat cgttcaaatc aaagataaaa
     8881 agttcaaaaa atttattcct gaacaggaaa ttctttcttc catcagaaaa attggtgagg
     8941 aaatcaataa aaccctgaaa gataaaaatc cactctttat ttctgttctc aacggagctt
     9001 tcatgtttac gtccgactta atgaaggtgg tgaacattcc gtgtgaaatc accttcatca
     9061 aactttcctc ttacgaaggg ctttacagtt caggcagtgt gaaagaagtg atgggattga
     9121 acgaaaatat cgtaggccgt aatgtagtga ttttggaaga tattatcgac acgggactca
     9181 caatggaaca aatcattgat tcgctcaaag caaaaggtgc cggagaaatt cgggtggcaa
     9241 catttttaca aaaacccgat gccttgcagc gcaacatcaa aatagattac attgccatga
     9301 aaattcccaa tgaatttatt attggctacg ggctcgacta caacggatat ggaagaaatt
     9361 taaaagatgt ctatactttg ataacggatt aatacagaaa acagattaac aaatgttgaa
     9421 tatcattatt tgcggagcac cgggaagtgg aaagggaaca cagagcaaac tgatagcaga
     9481 aaagtacaat ttaaaacatt tatccaccgg agatatgctc cgaaatgaaa ttaaacttca
     9541 gacagaaatt ggcaaaaaag ccgaacagtt tatctccaga gggcaattgg ttcccgacga
     9601 aatgattgtt gccatgctcg atcatgttct cgacaaggaa atgcaccatt acaagggcat
     9661 tattttggat ggttttcccc gaactttcgc tcaggctgaa gctttggaaa aactgctgaa
     9721 aaaaagaaat gaagagatta gtctgctcct cgacttgaaa gttaacgatg aagaattaat
     9781 tagtcgattg cttaaacgcg ccgaaatatc gaacagaagt gatgacaaac tggatgtgat
     9841 tcgaaaaaga ctcgaaatct actatcaaaa tgccgacgaa gttaacaatt tttacaaaaa
     9901 aacgaataaa tatgtagcca tagaaggcgt tggcacagtg gaagaaattt tttctcgtat
     9961 ctcgcaaatt atcgattcaa aaataaataa ctgataacct ttgtctttca ttaaattcct
    10021 tttcccaaaa tgtcaggttc aaactttgtc gattacgtaa agattttttg ccgttcaggc
    10081 aaaggcggtg cagggtccac tcattttcat cgggaaaagt ttattcccaa aggtggccct
    10141 gatggcggcg atggtggtcg tggcgggcac gttattttgc gtggaaacaa aaattactgg
    10201 acgttgcttc atctgaaata tgcccgacat gtatttgccg aagatggaga aaatggaagt
    10261 aaaaaccgta gctttggcaa ggatggaaac gacaagatta ttgaagtacc ctgcggaacg
    10321 gttgcatacg atggcgaaac gggcgagttt atctgcgaca ttcaggaaga cggtcaagag
    10381 tttgttttgt tgcaaggcgg aagaggaggt ttgggaaatt ggcacttcaa aagcgctact
    10441 cagcagacgc ccagattttc tcaacccgga gaaccgcgaa aggaaagaac agttattctt
    10501 cagttgaaaa tattagccga cgttggtttg gttggatttc ccaatgcggg aaaatccacg
    10561 ctgctttcag tcgtttctgc agcaaaacca gaaattgccg attatccttt caccacactt
    10621 gtccctcaac tgggaattgt ggcttaccgc gacaataaat cttttgttat ggccgatatt
    10681 ccgggaatta ttgaaggcgc agccgaagga aaaggactcg gacttcgctt tttacgacac
    10741 attgaacgta attccatttt actgttcatg attcctgccg aagaggttga tattcgggaa
    10801 aaatacaata ttttgctgaa cgagctgaaa aaatataacc cctccttact ggaaaagtcg
    10861 aaaattctcg ccatcagcaa aagtgatttg ctggatgcag aactgaaaga ggaaatgaga
    10921 aaaaatttgc cggccgatgt gccgaccgtt tttatatctt cacttaccgg cgaaggtatt
    10981 cttgaactta aagatttgat ttgggatgaa ttgaataaac cttccaacca aataattgaa
    11041 atttctcacg ctcccattcc catctcgaat caggaaaatg aagaagaatt ggaagaagac
    11101 tttttccccg acaacatttc gtctgatatg gaagaaaccg accgttttga aaaaatggat
    11161 tgggaggatt gggacgaata atcttcgttt tttgaaattt tctaagtttc ccaccaatat
    11221 ttaaagattt ttcttgcctt caaaaaaggc atcagaaaaa attttgtcat cgcctcagct
    11281 ttttttgcta tttttgtaat gcctgagaaa cgaaagttac agtatcttct tttaagttcc
    11341 gttgtcgaat tctttaattt tgaaattata ctgacatgac caataaatgg atatttaagt
    11401 ctctctccga agaagaaaag ataattcaaa aaaatctggt agaaagtttg agcatcaacc
    11461 ctacccttgc ccaattactt gttcaacgcg gcatccagac ttttgaagaa gcacgcagtt
    11521 ttttccgtcc cgatttggct gagcttcatg atcctttcct tatgatggat atggacaaag
    11581 ccattgaaag attgaacaaa gccatcgaca acaaggaaaa aattctgatt tacggggatt
    11641 atgatgtgga tggaacgact tctgtggcat tggtctatca atttttgaaa aaatattatt
    11701 ccgatataga tttttatatt cccgaccgct ataccgaagg atacggcata tcgtttcagg
    11761 ggattcaatt tgccgccgaa cagaattttt cgttgattat tgctttggac tgcggaataa
    11821 aagccacaga aaaagttaaa catgcgctca atctcggaat cgatttcatt atttgcgatc
    11881 atcatactcc cgacaaagaa ttgcctcctg ctgtagctat tctcgatccc aagcgtgaag
    11941 actgccctta tccttataaa catctttcgg gttgcggagt gggatttaaa ctcctgcagg
    12001 ctttcaccat gaaaaaggga atcgattttt cagaactcac acctttgctc gatttagtag
    12061 gactgagcat agcttccgat attgttccca taaccggaga aaaccgcatc cttgcctatt
    12121 atggtttaaa aagacttaat tccctgcctt cattggggat ccggagtatc atcaatgtat
    12181 gtggacttgc cgacagagaa attaccatta gcgatattgt gtttaaaatt gggccacgca
    12241 tcaatgccag cggacgtatg aaatcagctc gtgaagctgt ggaattactt attgccaacg
    12301 atgcagaaat tgcacgacaa aaaagtggaa atatcaatga atacaacaac gaccgtaagg
    12361 atatagacaa aaatattacc gacgaagcca tttccatcat caactcgcag gatgtttaca
    12421 aaaaacgcaa atccattgtg gtatatcaac ctcattggca caaaggagta attggtattg
    12481 ttgcgtctcg tctatccgac gaatactaca aacctaccgt tgtcctcacg cgttcaaatg
    12541 gcttgatttc cggttctgct cgctcggtgc cgggtttcga tttgtacaaa gccattgatt
    12601 cgtgcagaga tttgctcgaa aatttcggcg gacacatgta tgcagccggt ctttctatga
    12661 aggaagaaaa tattcaggct tttaccgaac gcttcgaaca atttgtttca gaaaatattc
    12721 tggaagaaca aacttatcct caattggaaa tcgatgctgt tcttgaattt aaggatatta
    12781 ctcccaaatt tttccgaata ctgaaacaat ttgccccttt tgggccggaa aatatgaaac
    12841 ctcttttcgt ttccaaaaaa atctttgatt acgggacaag ccgactggtg ggaaaagagc
    12901 aggaacatct gagaatggaa ctgatcgact caagctcgga aaacgtgatg aatggaatag
    12961 cttttggtat gcacgaatac aatgatcact tgaaggccct ccatccgctt gatatttgtt
    13021 atacactgga agaaaacaat tttaatggaa attctaccat tcaattgatg atcagagata
    13081 taaagaccga agaatattaa ttgaaacttc gctcgaaaag actgtgcttt aataagttta
    13141 atcttgtttg ggctcttctg aaacacattc ttccgaagaa acaggcgaga ccgtctgctc
    13201 gggctgcgga acattgggat gttcaatatt ttcgcccttt ataatgaagc gtttataata
    13261 agacaatatc aaagacgtga agggcagtgc aatgatcatc cctgcaaatc ctaacagcga
    13321 accccaaatg gacaaggaaa gcaaaatcaa agccggatgt agtcccgtat attttcccat
    13381 gattctcgga actaaaatta aatcctgaat cgattgcact atcaaccata ctgctgcaat
    13441 gctgagagca atcatccaaa aattctgatt ggtttccgaa gctttcagca aagccataaa
    13501 agcacacgga ataataccca gcaactctag atagggaaca agattgagaa taccaatgct
    13561 caaaccaatc acaattgcca aaggtaaacg aatgatactc agcccaatca taaaaagaat
    13621 tcccacacac atggctatta aagcctgccc tctgaaataa cgattcatcc cgtcctgaat
    13681 atcctgaaca atattggtaa tcaaggggcg gtatttcatg ggaacaagtt caatccattt
    13741 ttcggaaatt ttctcgtaat ccattaaaat aaagaataga taaagaaaaa taattactat
    13801 cacagcaagt cccagaataa aattcaaaga atttccgaca aagttccata cttctggcaa
    13861 taatttttga acaagggaca taaatttttc gctgcctaaa agtgtttgta aatcaagttg
    13921 cctgaaatat tcagacagct gattttgcca ggcaataggc aaaaaagaat ttacatccac
    13981 atttctgaaa aaagaattga taagcaccac cgctttttct atttcattga tgatcatcgg
    14041 aatcaacaga aaacccagac ccgtgagaat aattatgaaa agcagcaagg ttgcaaaaat
    14101 agctacaaaa cgatacttga atttaagttt gtactgaaaa aaatggacta taggctgaag
    14161 cagataggcg agtaaccatg caatcaaaaa aggaagcaaa acaccgctta atcgctgggt
    14221 taagaaaaaa agagcggtaa gaacggtcag cgtgatgaca agacgcacca cccgatcaaa
    14281 tgtataaggt ctttcagttt gttccatcat attgcagaat tatagaaaag ttaaattttc
    14341 atttgactgc gttgaatttc attaaaacag ttacgacaaa ttggttcgta ttcctgcatt
    14401 tcgccaatca aaacccgctt gttgctgtcc accagtcgat gcgaaacata agccaatgca
    14461 ccacatctca cgcaaatggc gtgaaccttg aacacatctt ccgcaatagc gcacaatgcc
    14521 ggaataggac caaaaggtac accttgataa tccatatcga gacccgccac aatgacacga
    14581 atgccctgat tggccaactg attacatact tgcaccagcc catcatcaaa aaactgtgct
    14641 tcatcaatac ccactacgtc cacatttgca gacaacaaca gaatacttcc cgacgaacct
    14701 accggcgttg aacgtatggc attgcgatcg tgagaaacaa cttcgctttc cgaataacgc
    14761 gtatcgataa ccggtttaaa tatttctact ctttgcttgg caaactgcgc cctctttagt
    14821 cgccgaatca actcttctgt tttccccgag aacatcgaac cacaaatcac ttctatacta
    14881 ccacgtctcg atgcatgaga agtctgttct gaataaaaca tatcatttat tttgttgaaa
    14941 tttacgcttt ctttgtatat tttctatttc aattattgta actttaccgt ctaaaacgtt
    15001 ttaaagacaa tttttttata gtctgaaaaa tttcaggttg gaaaaataat atcttggaca
    15061 aaattaaaaa aaataacaag tactgaaatt cttttaaaga aaaaaatatg aacaaagatt
    15121 taatattggc tttgctccac aaaaacattc aggatttgga catgctcacc gaaagtttca
    15181 tggaaatgaa cgaacttcca ccggccattc ttcatcttgc catgcaaaaa gttgatgaaa
    15241 ttcaccaata tctcgaagaa ctgaatgaaa acaactcaac gaaaaaacct gccggtaaag
    15301 aaatttttcc ttcggaggat ttgaaagaca aaaaagaaaa caactttcta catttttctg
    15361 aacattcgac aaatgatttc gcagagccct cagaaaatgt ttcgcagcaa accgaaattg
    15421 aagcaataaa agaaatggaa tttcaagtcg gcgaagaaaa tattgcccaa gaaacagaaa
    15481 aaacggaatt ttctttcact gaaagtccaa ttaatgaaaa actaaccgaa tgggatgaaa
    15541 cagaaaaaga aacagcagaa attgagcaag aaccgctcga aaacgaaaaa aaccgatttg
    15601 tttctaccca gcccgaaata ggaggtgaaa ctaccgaaat aacagattcg gaagcccagg
    15661 aaaaggaaga accatcggcc aatgaaggcc aaaaaactgc accgacttca atcccgttgc
    15721 aggaaaaatt ttccaaaatt cccgataact ctttggggac tacatttgcc aatcaaaaaa
    15781 tcgacgacat aaaaaaagcc atcagcattg gcgaccgttt tcgatttcag cgtgaacttt
    15841 tcagggggaa tggcgaagat atgaacaaaa cactgaatta cctcaatcag ctggcaactc
    15901 tggaagaagc tgtttctttt ttgcaagcca aatacaaatg ggatggagaa aacgaaacgg
    15961 tgcaggattt ttttcaaatt ctcaaacgaa gatttttata atttcatcag aatataattt
    16021 attttttaaa ggtcttatgg aacccacatg tctgaatatt atactcattc gtgtttgtgt
    16081 atgcaataca tgtgggtctc atctgaaaag aatttttttt aaaaaatcat gagtcagcaa
    16141 aagaacaaaa cttcattggc ggggacgaca atcaataatt tttccgcatc caaagaagga
    16201 ggtaaacttt atgtggttcc tactcccata ggcaatttgg aagatatgac atttcgcgcc
    16261 gtaaaaattc tcaagaaagt gaatcttatt ttggctgaag acacacgcac cagcagtttt
    16321 ctgctgaaac acttcggcat agaaaccccc atgcagtcgc atcacaaatt caacgaacac
    16381 aaaacggttg aaaatattgt acaacagctg aaaagcggcc aaattttagc cctgatatcc
    16441 gatgccggca ctccggcaat ttccgacccg ggttttttgc tggtacgcga atgtgtagaa
    16501 aatggcatag aaattgagtg tttacctgga gcaacagctt ttgttccggc tttggtaact
    16561 tcaggcttgc ctaacgatcg cttttgtttt gaaggttttt tgcccgtaaa aaaaggacgt
    16621 cagcaacgtt taaaagaatt ggaaacagaa aaaaaaacga tgattttcta tgaatcgcct
    16681 ttccgcttgc tgaagacttt gacacaacta tcagaagtat ttggcgaaaa ccgtaaagct
    16741 tccgtttcac gtgaaatttc caaaatgttt gaagagacag tcagaggtac gttagccgag
    16801 ttgatacagc attttactga aaaaacgcca aaaggagaat tcgttttgat agttgccggt
    16861 tttgaagaat agctgtatct ttgcacagta ttttaaagtg taaaattttt attttaattt
    16921 attgattatc atattattaa tttttttcat tcaacaattc gtattttctc catactccaa
    16981 accattataa ttttccatcc ccatgaacaa aaatatttca taccgcaaca tcagagtttt
    17041 tagcgtaatt tttctggtag cttttctgac tgtttcgtgt ggaaaacaat cgtccgatta
    17101 caaaaagctc aaggcagaaa acgactcctt gctgaaagaa aaggaaagac tcgaacgcga
    17161 agtagatgac tatttttcga gtatcaatca gattcaggaa aatctggaaa aaattaaaag
    17221 taccgaaaat gttatctctg ttgaaccggc aggagaagaa ttatcacagg atgcacgcac
    17281 tcgaatcaac gaagacatca attaccttaa cgagttgctg caaaccaaca aggatgagct
    17341 ggcacggctg aagtcgagat tgagcaaaag ttcattaaaa ctcaccgaac tggaaagaac
    17401 cattacccgc ctcacaaaaa cattggaaga agaaacggcg cgcattgctt ttctggaaaa
    17461 aaagctggcc gaaaaggaca cccttatagc tcaaatgggc agcaccatca gccaaatggg
    17521 agaaaatatc aatacactga ctaccgaaaa caaatctaag gaagaaaaaa ttaaacaact
    17581 cgatgaaaat cttcattcgg catggtatgt ttttgggtca cgaaaagaac tcattgatca
    17641 gaaaatactt acatctaaag gaattttcag aaaagccgaa gtattaaaag actccttcaa
    17701 caaggattat tttgtcagga ttgatgcccg caatgccaaa accattccgc tgtatgcttc
    17761 caaagccaaa atactcacta cacatccgac ttcatcttat acaattgaaa aacaagatgg
    17821 caagttggtg ctcatcattg tcgatccgac tgctttctgg agtgtgagca agtaccttgt
    17881 tatcgaagtt gattaacaac atttttttgt ctgaaagaat tttaaaaaaa caggcgtcgg
    17941 gaaacttcaa atcctttttt aggctggcgg aataatcaag tatgattttt agaaatttat
    18001 ttctgaaaaa tgctaccgaa tgaataacaa gcatccttac cgatacgttt ttctcgattt
    18061 ggacgacaca atctgggatt ttcatgccaa cgcccgactg tcgttgcaaa aaatgtacga
    18121 acagcagacg ctcaaccgtt attttgaaaa tttcgacgaa tttttccgaa tttatgccat
    18181 gcgaaacatg gaactttggg aaatgtacgg aaaaggagaa atcaccaagg attttttgat
    18241 gttcgaacgt tttcgctatc cgcttgccaa aatgggactt gacgacttgc aactggccga
    18301 agccattgga aaacaatatc tggaaattct ccccgaacag acgactctgc ttcccttcgc
    18361 ccgtgagctg ctggattatc tccattccaa gtatccgctg acactcattt caaacggatt
    18421 tgtagaagtg caatacaaaa aactgaaatc ctgccaaatc gaacattatt tctctcacgt
    18481 ggtactttcg gaacaagccg gcgcactcaa acccgaccca gaaatattca actacgctct
    18541 tcacctgaat caggcctcac ccgaagaagc cattatgatt ggcgacagtt tttcggccga
    18601 tattgtgggc gccaaaaatg caggaatcga ccagatttat cttcaaaaaa acaatatgct
    18661 gccttatacc aaacaacagg ccacttttat cgtaaaatcg ctcgaagaaa ttttttcaat
    18721 tttgtagcag acaaaattcc ctgcatgttt atctacaatt caaaaaaagc tcaaaaaatt
    18781 tattttttga cgaaaaccaa aaaaagcact atctttgaac gtttttaata gttaaaacca
    18841 ttgcatcatg aatacaaacg aagacaattt actcgaatac agcgatgaag aagccgtaaa
    18901 attcattcta aagtctcttc cggccgaagt taaagaccgc atcgacgaag atactgttca
    18961 gtacgtgttg gatgtgattt acgattttta caacgaaaaa ggttatatcg acgaagatga
    19021 tactgccgaa gaaaccagca ttgacgaaga agaaatgtat gaatacattt tggaaaacgc
    19081 cttgaaagat gaaatcaacc ttacggaaga cgatattcag atgattttgg atgctgaatt
    19141 tgattacggc aaatcattgg gtatttacag agaagaataa tttcgttttt cacataccta
    19201 ctttttccct caacaccaac gcatgcaggt tttgcttggt gtttttttat ctttggtatt
    19261 tttgtaaaat aacaaaaaac atatgtttta tgtgatttat ccgcttttgt ggttgttgtc
    19321 cctgcttccg ctgaaagtgc tttatgcctt ttctccatta atgtatttcc tgatttatcg
    19381 tgtagcaggt taccgaaaaa aagtggttcg acaaaatctt caaaattcat ttcctgaact
    19441 ttcggcatcc gaaagaagaa aaatagaaaa gaatttttac cgtttctttg ccgatttgtt
    19501 gcttgaaatc gttaaacttc ttcacataag cgacgaagag ttgctgcaaa ggatgaaatt
    19561 tgtcaatgtt gaggagtttt ttgaatgttg tcgtcaggga aaatatccaa ttttgatgct
    19621 cggccattat ggaaattggg aatggatatt gaccatcagc ttgttaatgc ccgaaaattg
    19681 cgagtctttt accgtgtaca agcggttaaa aaacagaaag ttcgatcaat tcatgaagtc
    19741 gcttcgtgaa aaacggggag ctgtaatggt ggagaaacgc aatctggcaa ggagtatgat
    19801 acgttgggca aaagaaggaa aatttggcgc ttacggattt attgccgacc aaagtccgag
    19861 tggaaaaaat cttcgaaatt ggacaacttt tttgcaccag ccgactgcaa ttttgcccgg
    19921 tgccgaagaa ttggcaagaa aattcaatta tccggttttt tatgcacgcg tgagccgacc
    19981 aaaacgtgga tattattcct gtgagatttt gcccatagcc cgagagccac gaaccacttc
    20041 cgacaatgaa atttctacac gtttcatgca actgctcgaa gaagacatct tggctcatcc
    20101 tgaactttgg ctttggtcgc atcgccgatg gaaaattaaa cctctgattg atgaggtgaa
    20161 aaaagataat tgacaatcgc tttcgctttg acacaaacat cagctgttgc agcaaaacga
    20221 cagattttga ttttttttct gaacgtctga atgttaatcg acattttaaa aaatttttta
    20281 aaatcaaaac cgcaaaaaac aaataattct tttataattg actatgcaag tttcggtagt
    20341 gatattgaat tggaacggga aaaattattt gcagcaattt cttcccatac tgttggaaaa
    20401 gacgcgtttg tcgtatgtgg aaatagtggt ggccgacaat ggttctacag atggttcact
    20461 tcagttgttg aaagaacagt tcccaatggt gcgcatcatt ttattcgata aaaattatgg
    20521 ttttgccgaa ggctacaata aagccttgtc ccaaatcaaa tcggattatt acgtgctgtt
    20581 gaattccgat gtggaagtta cagacaattg gttgctcccg ctggtggatt atatggaaaa
    20641 acatccacaa actgcagctt gccaaccaaa aatactttct tattttaatc gaacacattt
    20701 tgaatatgca ggtgctgccg gcggttttat cgattatctg ggatttgcct tttgtagggg
    20761 acgcattttt ggagaagtgg aagaagacaa gggacaatat gatgacccga taaatgtttt
    20821 ctggacttcc ggagcctgta tgatggttcg tgcagggctt tttcataaaa tgggcggatt
    20881 tgatgcagat ttttttgccc acatggaaga gatagatctt tgctggcgat tcaacagtcg
    20941 tggctatcaa ttgacctgtg ttcctgaaag catggtttac catatcggag gaggcacttt
    21001 gcaatcggaa catccattca aaacttacct caactttcgg aataatttgt tgatgcttta
    21061 taaaaatctg ccgaaaaagc aattaaaaag cgtgtttttc aagcgagcca ttctcgatta
    21121 cctggctgtt tttcaatttc ttgcctctgc caaacccaaa aatgcacgaa gcatactgaa
    21181 agctcgctcc gatttcagaa aaatgaaacc aaattttctg gataaaagaa aggaaaatat
    21241 cctcttctcc accgcaacat cctttcccat gatttatccc cgaagtattg tggtggatta
    21301 ttatttgaga aagaagaaaa aattcacaac actaaaattc gatcaaaaac caaactaaaa
    21361 aatattttaa aaactgattc ttaagcttcc acgaatagca aaattgtcgc cggaaaattg
    21421 cttaatatcg gtcaatcgcc agattgcctg taaattgaat agatgcagaa tattggccag
    21481 tccgacgcca acttcggtgt agggacgatt aaaaggataa acgtaagccg gaaaatccat
    21541 gaccgacaag tggtcgttac tcatttttcc gtaaccgaac ttaacggaaa acatttcgcg
    21601 catgttcagg tacttgagga gcggaatatg atttaatatc agcccgttca taatcagttc
    21661 tccctgaaaa tcaaaatatt tgtcggctgc aaattgcata aaattcatca tgctgaaacg
    21721 gtaaaatccg tatccgcctt ccgctccggg ttgatatgcc aacagtggat aaggaacgct
    21781 tccaaaaata cttccggctt ccatccaata attgacttgt cccaaatcga actggacatt
    21841 ttgtttaaga atacccgaaa cttttccgta gtatttttca gtatttccaa gttcatattt
    21901 ccccacttcc aaaatgccgt aaattattgg gttgcggtta ttgatataaa ttcgctgtaa
    21961 atggtcttcg tatttttttt cgttgaacga aaaacgtgtt gaaaaggtaa aagattgttg
    22021 ttggaaattg ccaatatcga ctccgtcctt ttttaacggt aaagcatcgg atggaagctg
    22081 ctgcaaagac ctgaaataca atccggattc tatattcctg ttccagtcgc cggagaaaga
    22141 aagcgtccat tcttttcttt cgttcaatcg accggcactg cgaagggcaa aaatggtgcc
    22201 ggcaatatct tcatcgccac ttacaagcgg attttcgaga tacagaaaat tattgtaatt
    22261 ataatctata cgacggtaat cgttcgtgta attcaaaccg ataatgcgtc ttttttccgc
    22321 tggcaattta tattgtgtga aacctgaata ttttatggct tcgtttttga atccgtaacc
    22381 cacaaaaccg ccaacagtaa agtttttcca cattttttcg ttggttctga agggcaatgt
    22441 cagtctcagt ccttccacat cggtagtgcg ggcaagatat tgaatttttc ctatatcaat
    22501 ttttcctaca ggaatgtaac ctgtaaaaat aatgtcggcc aaccatttag ctgcattcat
    22561 caggggtgtt tttcctaatt gttgaagctt ctgattgaga gtttcttcgc tgtacccgct
    22621 ttgagcaaaa ctttcgggaa tttggatact tccggtattg taaattgtac ttcttttaaa
    22681 gaaaatctga gaaccggccg gaacactgtc ggttaaaatg tcaaggttca ataccagtga
    22741 aagctgatct ttgttacgca cccagcgttt tgacgaaaag ggcacaaatt cctgctttat
    22801 gctcaggtgt cgaataaagt tgatgttggc ttccgaaggg agttctgctt caatgtaggt
    22861 aagtgccagc gaagccgaat cgagacagaa ttttccattg aaagccggat ttctcggatt
    22921 tcggcttctg aaatgtattt cgtattgttt tcccgaagat gtaaccatgc tgtcggcaag
    22981 gtaataattg taatacatat tgccaatgct tgccagtggg ctgataatgt ttttattgaa
    23041 aacagggatg gaattttcgt aaaaattcag tgatggattg agttcaccaa aaagctgctc
    23101 caaaattttg ttgccttctt cagaggaaga aaagattttt tttgaaagct ctgtttttgc
    23161 agtggaagtc aattgatatt ttatttcgtc catgtaaagc ggaacaagca aagaagaatc
    23221 ggaagagagc agatttcctt cctgaagctg gaggaaaatc tgtttgctga tggagcgctg
    23281 ttgcgaacga gagagaaaaa tagcattttg ttcggttgcc aaaacttcgt aatgaggcag
    23341 tttggtctca tcattcactt ttctcaacag tcggactttt ctcattaaat cgagagccgg
    23401 attagcgccg ggcaaaacaa aaacttcctg aagcagtgtg ttcgattctt ccatctcaac
    23461 agtcaggcca atatttgaac ccggttttat tttgatttcc gtctttttgt atccgatagc
    23521 cgaaaatacg agtatatttt cctttcccgt gtgtctgaga agaaaataac cttcttcgtt
    23581 gcttgttgtt ccaacggatg ttcctttgaa gtaaatgctg accgatgaaa gcgggtgctt
    23641 gtcgtatttg tcgataacct gacctaccac aattgtttct tgagcctgca aaccggcaac
    23701 gaatagaact tctgtcaaga gaattcccat cataaaacgg aaaagcaaca aacttttttc
    23761 agaaatccga cagattttta ttttcctcat ttttcgattg gttcaaggga tgatatttta
    23821 aaatggtttc tctaaggaac tgcacgtcga tatgggtata aagctcggtt gtagtaatgg
    23881 attcgtgccc caacaactgt tgaatggcgc gcaggttggc tccattttcc agcaagtggg
    23941 tagcaaaaga atgacgaaaa gtatgcggac tgatgttttt ttgaatgcct gccatagttg
    24001 ccaaatcctt cacaattttg aaaatcatgg ctctggtcag tttttcgcct cttcgattca
    24061 gaaaaagaaa atcttcatgt ccttttttga tttccaaatg attgcggtcg attttccaaa
    24121 gttcgatttg ctttatcgaa ttgggcgaaa gcggaacaag ccgctgttta ctcccttttc
    24181 cctctaccat catgtaccct tcgttaagat aaagctgcga aagtttgacg ttaatcaatt
    24241 cggaaacgcg cacgcccgaa ccgtagagaa cttcaatgat ggctttgttg cgttgacctt
    24301 caggttttga aaggtcgatg gctgaaatca tggcatcaat ttcttctacc gacaatattt
    24361 gtggcaaccg aagtccgatt ttaggacttt cgagcaattc gctcgggtca gtagaaatta
    24421 tatttttgta aaccaaaaaa cgataaaacg aactgatgct cgatatgatg cgggcttgtg
    24481 aacgcggatg aattcccaga ctaccaattt cgattatgaa atcacgcaaa agctcgatgg
    24541 ttgcatcttg caacaataca tgaaaatcgg acagatagct gttgagtttt tccaaatctc
    24601 tttcgtaagc ctgaatggta ttttcggaaa gtgctttttc aaacttcaga taataatgat
    24661 aatcggtgag taaggagttc atagacaatt tttaaaaccg ttgcaaagat acaaaatctc
    24721 gaaggaactt ttgtaggaaa gttaaaacta catttttatg caaaatattt aatttttctc
    24781 cattttgtca aacatcttaa aagtttttct aattttgtcc tccaacttgt tccatctttt
    24841 cgattgttta atcaatatac taaaaatcaa tatgctgtgt aaagtaaaaa ctttatttgt
    24901 cattttattt tgtgccatta attttgctct ttcagcttat gatgtaacag ggcatcgcat
    24961 tattgccagc attgcctatc agaatctcaa tgcagctaca cgcaatcggg tggacaaatt
    25021 gcttggtaag cagggaattg tttacgaagc gggttgggct gacgaaataa gaagcgacga
    25081 taattatgcc tattcttacc agtggcatta tcagaacttg aaagacagcc ttacatcgaa
    25141 agatttaaag tttttgctgg aacaacccaa agccgaaggc aaacatttgt tttttgcgct
    25201 cgattcgttg tccggtgagc ttaaaaagaa tcccaacaat gcggaagctt taaaatttat
    25261 cgttcatttt gtcggcgatt tgcatcaacc tatgcatttg ggacgtgttg acgatttggg
    25321 aggaaacaaa atcatggtaa agtggtttgg agccaaatcc aatctgcatc gcgtgtggga
    25381 tagtcagttg ctggaaagtt ccaagtattc ctattctgaa tacagtattt atctgcaaaa
    25441 caagtttgaa ccacaaagag ccacgtatca aaaattcagt ttgttgcagt cggtagaagc
    25501 ggtatatggg cttcgcaacc agatttatgc ttacgatttg ccaacggaaa cgaataacta
    25561 caagtacatt taccttttct cggataaact ggatgaaatg ctttatttag ggggcattca
    25621 gctggcaaat gttttaaacg gaatttactc gaaataaaaa aattattgat tgtaaggaag
    25681 acaacgacaa gcaggtgtca gactgtcaaa gaatttataa ctgaaagtaa tgctgagatt
    25741 agccaaaaga tcattctctt tggaagtaac agtagaaata ccatctataa aatctgtaaa
    25801 agtataattc cagcatagtt cggcaccaat agaaaattta ggtgtgattg catatttata
    25861 tcctattcca aatggaataa aagctgctgt ctcattgagt tttatcttat cgtatatcct
    25921 gtctggcgga ggttgagaaa tagccactcc atttttcttt acttcagcat tactgttcat
    25981 cacacctatg cccccaaaaa gataaagcga ataataatcg tagtaatttg aatgtatgcc
    26041 tcccaaaaaa gtatattccg cttgcaacga caagcttgaa acttcggaat taaatgaata
    26101 ttgtctggct tcgtttctgg aatttttatc gttaccgata aagttcccgt aattaaaaat
    26161 cagtttataa ccaagattat gtttaaatga ctgaccatag ccaaccgaaa acaaatatcg
    26221 ggtattcagc acatcccaat cgttaatacc gtttaaaaac tgttcataat gcgaaccacc
    26281 aacgtctcca aagcaataag caggcccggc agcagcaaaa aaaattgatt ttgagttggg
    26341 aattacactt ttacctatta atccattttt ctgtgcatgg accacgaaaa atgaaatttg
    26401 tacaccaaaa agcaatatta tgatcaaaac ttttttcatg ggtgtgttgg caatggattg
    26461 tttgcaaaat taaaaatttt tttagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26581 nnnnnntttt ttagtgtttt cttagtattt tcttaatacc tgaaactgct tcccccaaca
    26641 aactcacgca aaagtgaagt aggaggaatt tccctgtcgg gaataggtac aaaataattt
    26701 aaaaatttaa tgagccgatg agcttcggtg tattcgtcgc aatattttgg gacttcggca
    26761 agcaaaggtt cagcatagcg ttttaacacg gcaaactcaa aaagcaaggc agagttgaac
    26821 atgacatcgg cattttcctg aaacgggaaa atccattttt cttcaccttt tctgacgctt
    26881 tcccaacgcg caatggtttc ccttgcggaa taatttctgt accttgaatc gcgaataatc
    26941 cttcgcaaca atcgagtgtc ggttgtaggt atccaattgt gattgtcgat ggagatggtg
    27001 gtcagagccg aaacgtatat tttgaatgta gcttctgctg gaatttcggg aagaagtttc
    27061 ggattcagtg catgaatccc ttctaaaatc accagactgt cttcagcaag tttcagttta
    27121 tctccacgat aatagcgccg accctcttca aaattgaaac tgggaagttc aatttcttta
    27181 ccttcaatca gttctcgaag ttgttgattt aacaactcta aatcgagtgc atgcagatgc
    27241 tcgaaatcta actctccatt ttcatcacga ggtgttttgt cgcggtcaac aaaataattg
    27301 tccagcgaaa ttgctaccgg tttgattcta caaacggcca actgaaccga aagtcgctta
    27361 ctgaaagtag tttttcccga agaggaaggc cccgaaataa gtataaattt tactttgtct
    27421 ttccgttggg caatggtgtc agcaatagta gccacctttt tttcgtgtaa agcttcggaa
    27481 atttttatca gtgaaaaagc ctgcttatta cgacaagcaa cgttgaattc acctacattg
    27541 ctcagattca tgattttgtt ccattccaca tattctttaa atatatcata aagttttggc
    27601 tgtggaacaa tttcttccaa ttctgttgga ttctcccgat tcggaacacg caacagcagt
    27661 ccgtcgtaat aaggaatcaa atcatacagg ttcaaataat ctgtcgaagg cagtaggacg
    27721 ccgttgtaat aatcgatata atcatcgatt ctgaaatatc ggcaatacgg attacctaaa
    27781 gtctggaaga gaacaatttt atctttttgg tcagcaaata gcttgattac ttcagaggtt
    27841 tgtttttctt cacaaattat ggttttacct tgagagataa ttaaattaat tcgttcttta
    27901 attttatcga ccaagctttg tgtgagaggt tcatcgatgt tgttcagtac gcaataataa
    27961 cctttcgata aaggatgttc gatgcgaatg ttgcctttgg ggaataattc agaaacggct
    28021 ttggcaacta ccattgtcag cgtgcgaaca taaacgcgca tgcccgaaga ggtgcttaaa
    28081 tcaatgaact caatgtcttt gggcttataa atcaaaaaat ttagatcttc gaccttataa
    28141 tttacgcggg ctgcgacaac ctgatatttc aattgaatgt ttaacgaccg gtaaatttca
    28201 attagtgaag ttcccaaggg gaaatcgtga tatgaacggg tatttttgca ataaatggtt
    28261 acggtttttc ccatgagtaa gataaaaaaa ttgttttcaa ttcaatttaa aatatgtcgg
    28321 taaaattata tatttttttt gcatttcttt caagaaaaaa ttttttaaag tacttttgca
    28381 aaaaaatctg taattagaaa taaaatgcat tacacatttt atgatttact tacattaatt
    28441 ggttcattgg ggttgtttct ttatggaatg aaaaccatga gcgaaggact gcaaaaagct
    28501 accggcgaca cgatccgttc cataatggct gcaatgacaa gaaatcgttt cacggggatt
    28561 cttaccgggt tgcttattac ctcacttatt cagtcttctt ctgccactac tgtaatggtg
    28621 gttagttttg taaatgcggg attattgtct ctttatcaag caataaccgt cattatggga
    28681 gcaaacatag gaacaaccat aacggcatgg atgatttcga tttttggatt taaagttagt
    28741 atcgctgctt tttctattcc attgataggc atttccatcc cgcttatttt ctcctctaaa
    28801 agtcgcaata aatctatagg cgaattttta attggatttg ccttattgtt tatgggtttg
    28861 gagttattga aaaattcagt ccccgatatt cagggcaatc cggaagtctt aaattttctt
    28921 gcaaaatata caaattttgg gtttggttcg atattactgt ttttactcat aggcaccata
    28981 ctgaccatta tagttcaatc atcgagtgct actatggcta taacattaat catgtgcagc
    29041 aaagggtgga tttcttatga acttggagct gccatggtat tgggtgaaaa tataggaaca
    29101 actattacgg ccaatctggc agctattcct acaaatgttt cagctaaaag agccgcctta
    29161 tctcatttaa tcttcaacat ttttggcgtt atttggatgt tgatcgcttt caggccattc
    29221 atcaatttgg ttacaatgtt gataactcaa tttgggccgg gcgaccctac tcaattaact
    29281 gctttctcca atagccttga cccagatacg ctaaaagtta tttcttcggg aagtgaaaat
    29341 ctttcacccg aacaaattgc agtcaggaat caacttgaaa attatcaaat agcaacctca
    29401 tacggcttgt cattatttca taccttattc aacataacca atactttggt tttgatatgg
    29461 tttgctaaaa ttatagagaa aatttcaacc ttgctaataa aaaagaaaga tacggatgaa
    29521 gaatttcagc ttcaatttat ttctaccgga ttgctttcta cttcagaact ttcacttata
    29581 caggcatgga aagagattaa ggtttatgct gaacgttccc accgtatgtt ttcattggta
    29641 agagaattgc ttcaagaaaa aaatgaaaat gattttataa aaaaatttag tcgagttcag
    29701 aaatatgaaa atataagcga ccgaatggaa gttgaaatag cccactacct tacgaaagtg
    29761 gccgaaggca aattaagtga aaaggggaaa tttcagatac aaaccatgtt gaaagtaatt
    29821 tcggaaattg agagtattgg tgatagttgt tacaacttgg ccagaactat caagcgaaaa
    29881 aaagacgaca aagctgaata taccaaagaa atggatgata atttgaatga aatgatgaat
    29941 ttggtagaag atgccctctt acaaatgcga gcagctatcg acaacaacca tgtatcaaaa
    30001 gaagaattga atcgcgctga aaatttggaa aacgaaatca ataatttcag gaatcaactc
    30061 aaaaataaaa atattgatga cgttaaagaa aacaaatacg attatcaggc aagcgttaca
    30121 tacatggata tgcttgaaga atgcgaaaaa cttggagatt atatcgtaaa tatagttgaa
    30181 gcactttacg aagcttccaa ataaatgacg aaaggaatca aattccatgt ttaatccgtt
    30241 tccatctctg ccgatcgacc aaaagtaatg ttatccattt ttctacctcg cacaaaattt
    30301 cttttcataa aaaagccgta attttgcttt tgaaattgac aagtttttca actctttctt
    30361 ttttatatca ataacttgaa aagtcgaacg aaaaaaatta tcgtctaaca ctactctcat
    30421 ttaaaatgga ttacatattg attatttcag gttttgctgt tttggttttg ggtgctaact
    30481 tattagttga tggtgcaagc agcttggcca agaaattaaa gatatccgat ttgatcatcg
    30541 gacttactgt ggtggctttt gggacttccg cccctgaact ggtggtaaat gtaatagctt
    30601 ctgtgaaacc taatgcaaca gatatagcaa tttccaatgt tattgggagc aatattgtta
    30661 atatctttat cattcttgga ttttcagctc tgtttcatcc cattaagtcg gagaaaacta
    30721 caactttatt tgatataccc ttaagctttt ttgccattgt tgttacattt cttcttgctt
    30781 ttacgggaga taggatttta tcaagaaccg atggacttgt tatgcttggt ttttttatcg
    30841 ctttcatata ttttaccacc cgcaggtcga taaaacatcc attgatacct ttggaagaaa
    30901 acatcaaaat tcgtaaaacg ggaatttcga ttttgatgat ttcttgtgga ttggcagccc
    30961 ttataggtgg ggctgaaatt attgtaccca gcgccattcg tatagcagaa aatttgggta
    31021 tcagtcaagc agtcattgga ttgaccatcg ttgcattggg aacatcgctc cctgagttgg
    31081 ccacctctgt agttgctgct atcaaaaaga attccgatat tgcacttggg aatattattg
    31141 gctcaaatat ttttaatatt ttcctgattt tgggtgtgag cagtgttatt cgcccattac
    31201 ccatctatag gggcatagga atagacttga ctatgggtgc acttggcagt gtgatggttt
    31261 ttgtttttgt ttttggaagt aagtcccaca gcataaaaaa atggcaaggt ttattgctga
    31321 ttttcatata tctaatgtac gcaattttca aaatttttta atttaaagta tccttaaaaa
    31381 attatttaac aactcctcgt tatgaatctt tttgatgtgt atccgctttt tgatatagaa
    31441 attgttaaag gacagggatg tcgtatttac gacaaaaacg atacggaata tctggatttt
    31501 tatggaggtc atgccgtcat ttccattggg cattcgcatc cctattttgt aaaaaagctg
    31561 gcggatcaga ttcagaaact ggttttttat tccaattctg tgaaaaacag tttacaggtt
    31621 gaattggccg aaaagctggg aaaaatagct ggttacgatg attattccct gtttcttatc
    31681 aattcgggtg cagaagcaaa cgagaatgcg ttgaaactgg cttcgtttca caatggacgc
    31741 agcaaggtga ttgctttcgg aaaaagtttt catggcagaa cttcggctgc ggtcggagtt
    31801 acagacaatc cagccattca ggcgcctatt aatcgaaatt ttgaagtaga atttctaccg
    31861 ttgaacgata cagaggcagt tcttcaatct ttgaaaaaca gagatgtttg tgcggtgatt
    31921 attgaaggca ttcaaggagt aggagggatt cagattccac agcctgcgtt tcttgaagcg
    31981 ataagcgcag cctgcaagga gaccgaaact gtacttattc tggacgagat tcaatcgggt
    32041 tatgggcgaa gtggaaaatt tttcgctcat caatatgccg atattcgacc agatattatc
    32101 actacggcaa aaggcatggg aaacggagtt ccgataggtg gagtaattat cagcccaatt
    32161 tttaaaccca cttacggaat gttgggaacc acttttggag ggaatcatct tgcctgtact
    32221 gctgccattg ctgtattgga tgttattgaa caggaacatc ttatggaaaa tgccttgcag
    32281 gtgggaaatt atttgataaa cgaactgcaa aaattttccc gattgaaaga agtcagagga
    32341 aaaggtttga tgatagggat tgaatttgac gaaccggtaa aaaaattgag agaacgttta
    32401 ttgtttgaga aaaaaatatt taccggtgtg agtggttcca atattatccg tttgttgcca
    32461 cctctttgtc tgactatgca ggatgcggaa cttttccttg atcgttttgc tgaagtgata
    32521 aaataatttt gtaatttctg ttagtttcat gaaaaaaccg atagcccttt tgtttatcct
    32581 tttttttgta aatagttgga cggttttctc tcagtctgtt tctaaagaac gggataaagt
    32641 cgtgatgaaa accggtgaaa tttattacgg agaagttgtt tttcgaaatg acgaaatgct
    32701 tattttgcaa acagacgaag gagttcgata tcagttttct attgcggaag tgaaagccat
    32761 ttacaaagaa acggtagatg gttctgatga gttagaaaaa atgcaacagg aaggtcagtc
    32821 tgatgaaata cagaactttt ttgcagtgtt ggatgcgtgt ggaggaatct cgagtgccaa
    32881 aaacagtttt gatgtttctt ctaatactca agtagcattt gttttgggga aagataaggt
    32941 tttttcggaa aattttttcg tgggtttggg tgtcgggtac aattcttctt atttaaagag
    33001 taattcgtca ctgaattttc tccccgtttt tcttcgcttg caaaatactt ttactaacaa
    33061 tcggatttcg ccttttgttc gcatggatgc aggatacgct ttttctatga cttccggctg
    33121 ggatggaggt gcattggtta aagcggctgc cggtatcagt tcnnnnnnnn nnnnnnnnnn
    33181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33241 nnnnnnnnnn nnnnnnnnnn nntgacttcc ggctgggatg gaggtgcatt ggttaaagcg
    33301 gctgccggta tcagttcccg tttaagttac aaaaccaaac ttttttgtgg tgtgtatgcc
    33361 ggtattcagc aattcggagg aaaactctcc gaaacaattt cttcaggcac ttattattac
    33421 gaaggtacaa caaacatgaa gcaattgggt ttgcaactcg ggattcaatt ctaaattcag
    33481 cagaagcagg aatttttcca ctataactaa attgctgata atgagtaaaa taaatttatc
    33541 tcaagttata attgctgcat taaatgaaca ggcgtgtgca gaaaaagctc agcatcacct
    33601 ccggtttttc aaaactgctc cgggcgaata tggcgaagga gacaaattta ttggtgtgaa
    33661 tgttcccgat cagcgaaaaa ttgccaaaaa atatttcaga gaaattaatt tgaatgagct
    33721 ggaagtttta ttaacgaatg aaattcacga atgccgttct acagctcttt ttatgctttg
    33781 ttacaaattt gacaaagccg gtgaagcgga aagaactttg atggcggact tatacctcaa
    33841 taacctatct tttgtaaata attgggatct ggtagatgct tctgctgcaa atattttggg
    33901 ttgttacttg atggacaaaa aaagagactt gctttacgac tttgttcaaa gtaacgatct
    33961 ttggaaacag cgcatagcca tcatcgctac gttttatttt attcgttgtg gtgagtttga
    34021 ggacactttc aaaatttccg aaatgctgct tcatcatcct cacgacttga ttcataaagc
    34081 ggttggatgg atgcttagag aagtggggaa cagagataag gaagcagaaa tcaacttttt
    34141 actaaagcat tatcggcaaa tgcccagaac catgcttcgg tatgccatcg aaaaatttga
    34201 tgaaggtttg cgccgacaat ttttaaaagg gtaaatttac ctataaattc ctattgcatt
    34261 cgttcaattt tattaggtgt gatcaaagat gcttttccta ctttttatag agtcggactt
    34321 tattttttcg tcggggaaaa aagattatcc aaagtccgga gatattattt taattctgga
    34381 gcgttattta aaaaattata gagtaatttt gtgagttcaa atgaatgctt cttcatgaaa
    34441 gcgacttaaa aattttccgc aaaatgaaat taaaagaaat tgcccaaata cttgggaatt
    34501 cacatttaaa agttcctgaa gttgaaataa attggttatt gaccgatagc cgagctcttt
    34561 cgtttccaac aagaagtttg ttttttgctt tgcgtacccg gcgaaacgac ggacatcgtt
    34621 acatacagga actttatgac cagcatttaa gatattttgt ggtcagtgaa acgcttccag
    34681 aatttgaaaa aatgcacgat gccattttca ttcgggtaaa cgatacgctg gaagctttgc
    34741 agcatttggc cgcagttcac agaaatgctt ttcaaattcc aattgtggga ataacgggga
    34801 gcaatggtaa aaccattgtc aaggaatggt tgtatcaact gcttcattcc gatttcaaca
    34861 ttacccgttc gccacgaagt tacaattctc aaatcggtgt gccgctatcg gtttggggat
    34921 tgaacaacaa taccgagctt ggaatttttg aagccggtat ttctcaaccg cacgaaatgg
    34981 aacgtttgga accaattatt cgaccgacca tcggaatttt taccaatctc ggcgatgcac
    35041 atcaggagaa tttttccagc ttgaaacaaa aatgcgaaga aaagcttaaa ctttttgctc
    35101 atgctcaagt tttgatttat tgttcggata atagattggt ggatattacg attgctcagt
    35161 cgaatttcaa aggcaaatta ttttcttggg gaagaaatga aaatgctgtt ttacaacttg
    35221 ttaaaacaaa aaaactatcc gattcaacgg aagtagttta taaattttca ggaaaggaat
    35281 cggctgtttc cattcctttt tccgacgatg cttctataga aaatgccatg cattgcgtag
    35341 cattgatgtt acatctcggt atcgatttgg aaaaaattcg tcaacgcata gcgaaactcg
    35401 aaagtgtgga aatgcgcctt gaattgaagg aggggattca cggatgcctt ttgattaacg
    35461 acagttataa ttccgatttg aattcgttgg caattgcgtt ggattttctc aatcagcagg
    35521 ctgcaaccaa aaatttgccg cgaacgttga ttctttccga cattttggaa agtgggaaac
    35581 agtcggatga tttatataat gccgtagctc agttggttaa aagtaaaagc atacagcggt
    35641 ttgttggtat aggcccagaa attgtcaaac atgccgataa atttgaggta gagcaacaaa
    35701 attttttcga atttacggaa gattttttga agtcgccact tatccacgaa ttgcagaaag
    35761 aaatcatatt attgaaaggt tcacggaaat ttcattttga aaaaatttcc gaaaagttgg
    35821 aactgattgc ttacgaaacc acattggaag taaatttgaa tgctttgatc aacaacttca
    35881 actattttcg ttccaaactc cgtcccgaaa ccaaagttat gtctatggta aaagcttttg
    35941 cctatggaag cggatcggta gaagttgcac gaaccttgca actccatcat tgcgattatt
    36001 tggctgtagc agtagccgac gaaggagccg aattgcgccg ggaaggaatt cgtatcccga
    36061 tagtggttat gaatcccgaa aaaggaagtt tcggaatgat tttcgagcat caacttgagc
    36121 ctgaaattta cagtttcaga attttggaat catttctgtc ggcagccgaa aaattgggcg
    36181 taaccgatta tccggttcac ataaaaattg acagcggcat gcatcgattg ggatttgaac
    36241 ctcacgaaat tgttcctttg atacaacggc tgaaagcaca aaacaacctg aaagtgcggt
    36301 cggtattttc tcatttggcc ggtgccgacg aaaaacgttt cgatgatttt acgcggcaac
    36361 aggtaaaagt tttcactgaa tgtgccgatt tgtttgctca gtcgtttgag catcccatta
    36421 taaggcatat tctcaactct gccggcatag aacgtttccc cgaatttcaa tttgacatgg
    36481 tgcgtttggg aatcgggcac tatggtgtca gtgcgttgcc caaagttaaa ctggaacctg
    36541 tttgtgcact gaaatccatt attttacaga ttaaaaatgt aaaaaaagga gaaacggtcg
    36601 gttatagtcg caaaggaatt gccaacgaag acaaacgaat tgccgtcatt ccggtgggtt
    36661 atgctgatgg cttcgacaga aaattgggaa atggtgtcgg cgaagttttg attaacggaa
    36721 aacgcgccaa agtgatgggt aatgtatgta tggatttgac aatggttgat attaccggta
    36781 ttgaagcaaa agagggagat tcggtggaaa tattcggcaa caacctgtct gtcatcgaag
    36841 ttgccgacaa gttgaaaacc attccctacg aagtgctcac cagtgtttcc aaacgagtga
    36901 aaagagttta ttttcaagaa taaattcagt ctgttttggt atttaagttg agttttattt
    36961 tattttattt taaaaattag atgatcctta tttttgtaca aaatacaacc ccgccggttg
    37021 cacttttgca aagtgttgcc atattgtctg tccaattgaa aaaagaatgg ggcatacttt
    37081 gtttgacgga aaatgccgat ttggtttcag tttacgaaaa tcaatatggt gaatttttaa
    37141 aaacaaacgg attcccggaa gtgtctatat ttgtgagaca agcttgttgt gagcaacttg
    37201 tagaattttg tgaacaactg gaagcttctt ttcttttttt tcagttgcat gatgcatccc
    37261 accgatatct tcgtcggtcg ttaaattttt gtcgtgattt gcgtataccg tatttgtttt
    37321 tcaaaaattc cttttcggtg cttaatttgg agaaagttgt agttccgata ggttttttgg
    37381 aagaagaaat agaaaaagcg cagtttgcat ctgcttttgg gcgatttagc aatgctgaaa
    37441 tcattttgct ccctgccaac gattatgggt ctaaagctgc aacgacaacc gataaaatga
    37501 aaactatcct cgacaagttt caattgccct acacggttga gaaagcacaa tcggacagtt
    37561 ttaaaattga cagagaagca atagaaaaag cagttgaaat gggaggaggg ttagtcatca
    37621 tttctgcgtc gcgagattac gggctggatg atattctttt tgggcccaaa gaattgcact
    37681 tgatacggaa atctcccgtg ccgcttttac tggttaatcc gcgaagcgat ttgtatgctc
    37741 tttgcgactg aaaaattttt cagtgtttta tttcaaaaaa agttccccaa aaaactccgg
    37801 acgatggaaa tcgggttttt cggtttttat gggattccaa cttaaataat gtggcgattc
    37861 agttccatcg gcgcatttgt aaaaatttcc cagaattttt tcgggtaaat tattcgggtc
    37921 gatgcctatc agtttaaagg gaatttttac tgtcaactcc cagtcgaaat ttccttttat
    37981 ttcctcaaaa ggctgtggca cgatggaagg gaatctttca atggatttca tttctgtttc
    38041 cgaaagaggt tcaacttcat cgtttcggcc tttacgccgg gaagccgagc aagtcccaat
    38101 gcaattgaat tcaaaattaa tatatttctc actctcgata gttttgcaga aaaattccac
    38161 acaactgtct tcgtaaaccg ggtcctggtc gtgcgtgtaa acggcacgca gcgcattgca
    38221 caaaacaaag aatttgataa atacagcttc tttcgactgc gcaatgtgga aataagcaat
    38281 gggtttgtag ggaaactctt ttgcccagtt ttgggattca atggcggctt ttgttccgtt
    38341 tttttccagt atttcgctcg ctgttgttat gtctgctttg tctaaatccg gaatataacc
    38401 gatatgcaat gatttcatca gaatataatt tattttttaa aggtctgatg gaacccacat
    38461 gtctgaatat tatattcatt cgtgtttgtg tatgcaatac atgtgggttt cataattttg
    38521 ttttctttca ggagaattac gtcttacaaa gataactttc ttttttaaca aatcaatttc
    38581 aaaaatgttt cccgcattga aatttatttt cctttgcata aagcacattg gtagcttgga
    38641 agttgaatcc ctgcatacaa aaatgtcaga aaattgtatt atttttgtgg aaaatttcag
    38701 aaataaatag gaatggcttc attctattaa aaataactga tttttaaaac tattttaaga
    38761 acattaagta ggtaagggaa acgaaaaatt tatctttttt gaatcatttt tcggctttac
    38821 caacaattac aatataaaac cagacaatta tgaaagtatt gaaatttggc gggacttcgg
    38881 taggttcggc acagcgcatg aaaaacgttg caaaactgat ttgcaatggc gagaaaaaaa
    38941 ttgtggtttt gtcagctatg tctggaacca ccaacagctt gcttgaaatt gcggattatt
    39001 tttacaaaag taatcttgac ggagctttgg aacatatcat tacgctggaa aacaaataca
    39061 gcgatgtggt ggaagaattg ttgacaaccg aagaaaaacg gcaggaagcc cgttctattc
    39121 tttcgtctca tttcgattat ctgcggtcgt ttgccaaatc ggtttttacc attttcgaag
    39181 aaaaagccat tgtagctcag ggagaattgc tttcaaccgc actttttcaa ctttatctcg
    39241 aagaaattgg tgaaaattcc atacttctgc cggcgctcga atttatgcgt atcgacaaaa
    39301 atgcagaacc ggacaatgcc tatatcaaac agcatttgaa cgatttgctc gaagcgcatc
    39361 ccggtcatga aatatatctt acgcaaggat ttatttgccg caatgcttat ggcgaaatcg
    39421 acaacctgca aagaggagga agcgattata cggcttcttt gattggcgct gccgtaaatg
    39481 ctgaagaaat tcaaatctgg acagatattg atggcatgca caataacgat ccgcgttttg
    39541 tggaaagtac caaacccatt cccatgcttc attttgaaga agcagccgaa ttggcttact
    39601 ttggtgcaaa aattcttcat cctacctgta ttttgcctgc taagttggct aatattcccg
    39661 ttcggttgct caatacaatg gaacctgaag ctgccggaac gcttatttct acacggatga
    39721 ataagggaaa aataaaagct gtggctgcaa aggatggaat tacggcaata aaaattcagt
    39781 cgggacgaat gctgttggct tatggttttc ttcgcaaggt gtttgaaatt tttgaatcct
    39841 ataaaacgcc tatcgatatg attactacat cggaagtggg cgtttcggtt acaattgaca
    39901 acacgaaaaa attggacgaa attgtcaatg atttgaaaaa acttggcacc gtaactgttg
    39961 atcacgatat ggttatcgtt tgtgtagtag gcgatcttcc tttcgaaaat gtcggctttc
    40021 aggcaaaggt tgttgatgca ttgaaagata tacctcttcg aatgatttcg tatggcggca
    40081 gcaattacaa tatttcattt ttgataaaag ctgaagacaa aaaacgtgct ttaaacgctt
    40141 tgagtcaggc tcttttcagt gaagaatagc aattcccgaa acctgaaaga gaacgttttt
    40201 tatattttta tttgtatgat aaaagcaaaa tatcctgttg aaaaattttt aaaactgcaa
    40261 acgccgtttt attattacga catggacatc ctgcggcaaa cgcttcagga agtgaatcga
    40321 cagattcaga aaagaaacta tgtggttcat tatgcgatga aagccaatgc aaatcccgta
    40381 ttgcttcatg aaataaaaaa tgccggtttg ggggccgatt gtgtgagcgg aggtgaaata
    40441 aatgctgcca ttaaagcagg atttccgaaa gaaaaaatag tttttgccgg agttggaaaa
    40501 gccgactggg agatcaatct tgcgttggat gaaaacattt tttgttttaa tgttgagtcg
    40561 attcccgaaa tggaagttat caatcaattg gctgctgcca aaagtaaaac agcttctatc
    40621 gccttgcgta tcaatcccaa tgtaaatgcc catactcacc aatatattac gaccggcctc
    40681 aacgaaaaca aatttggaat taacctttcc gatttggata cggttttgga cttgctccca
    40741 cagttgaaaa atttaaaatt aatcggtatt catgcgcata ttggctcaca aatcaccgat
    40801 atgacatcct ttcagagttt gtgcgtgaga atcaacgaaa ttcagaatta tttttttcag
    40861 cgccacatca ttttggagca tattaatgta ggtggaggtc tgggtattaa ttacgaacat
    40921 cccaatcatt ttccgatacc tgattttgag agttatttca atgtatttgc cgaacatttg
    40981 aatcttcagc cgtatcaaac actacatttc gagctgggac gtgccattgt agcgcattgc
    41041 gggagtttga tttcgagggt gctttatgtc aaacagggag ctaccaaaaa atttgtcatt
    41101 ctggacgccg ggttcacgga tcttatacgt ccggctttat atcaggctta tcatcgtatc
    41161 gaaaatttga cttccgattt gcctgaagaa gaatatgacg tggtcgggcc aatttgtgaa
    41221 tcttcggata ctttcgccaa gaattattct ttgaatggaa cacgcagagg cgatttggtg
    41281 gcattgcgtt ctgccggcgc ctatggagaa ataatggctt cacagtataa tttgcgctct
    41341 ttgccgaaag cttatacatc ggatgaaatt gctgcattgt aaaaacagcc gatttgatgt
    41401 ttttctttta aaaattttag tgaaaaaacc atttactgaa ctttaggaaa aaatcggcca
    41461 tcaattttga aaaatacgct tacccaaatg acagaaatat tcggattttc actttctttg
    41521 ttgacacttg ttttattgtg cggactgttt ttggtttttt gttatcagat ctatttttat
    41581 tgtcgttata tcaatggggt gattcgttac cggaataaaa tcagaaaggg gaaagttgcc
    41641 tttctggacg aaaaacccgg tgtctccgtt attatttgtt caaaaaacga agccaacaaa
    41701 ctgaaagaat ttctgccgtc cattcttgaa caggattatc ccgattttga ggtgattgtg
    41761 gtgaacgatg gctcaaccga cgaaaccgac atgttgttga tcgatttgtt gaaaaaatat
    41821 cctcgtttgc ggacaacttt tgtgccaatg gaagcaacca atttgagtac gaaaaaatta
    41881 gggatatcct tgggcatcaa ggcagcaaaa aatgattttc ttctttttac cgatgccgat
    41941 tgtcagccta aaagtaaaga ctggatttcg ttaatggcaa gaaattttgt tccaaaagtt
    42001 gagtttgttc tcggatatgg agcttatttt caccgaaaag gcttgttgaa caggcttata
    42061 aattacgata cttttttcat tgccttgcag tacatgggaa tggccatagc aggtaaacct
    42121 tacatgggtg tgggaagaaa tttggcttat cgtaaaacca cttttttaca ccacaatggt
    42181 ttttcggaga ttttaggatt aatttcgggg gatgatgatt tatttgtgaa caaaactgcc
    42241 aatgggaaca atacacgggt tgaaatttcg cccgaaagca tcactttttc cgaaccaaaa
    42301 accacatgga aaagctggta tttgcagaaa gcccgacatt tgtccgtttc gcctttctat
    42361 acttggcaaa gtaaactgat gctttttatg gaacctttca cacgcggctt gttttatgtt
    42421 ttttgccttg ttttgttgtt gcagggtgac atgataattt ctctggtggt ggctgccatt
    42481 tttttaattc gttatttcac acaactttat attgtcaatc gtacagcaaa gcattttaac
    42541 gaacgcaggt tttttctgct tttaccgatt tttgaccttg ttcttcccat cctttcgctc
    42601 agtattttta tcactggaaa agcgtcccga agaaaaaaca aaaaatggta atttctgaaa
    42661 gattggctct tttcaggctt tgaaaaaagt gaaatgaact cttccatatt ttcgcgtttc
    42721 catgaaatgg ggatgttgcg aaaagttgta tttggatgaa tgttcgagaa tgaaaattcc
    42781 ttccgttttc aacaattgct gtgaaaaaat catatccgga atttctttca gattttccag
    42841 ctggtaggga ggatcggcaa aaatcaagtc aaatcgtgag gctgtatgtg taatgtattt
    42901 gaaaacgtcg gttttcaaca aaatcaggtt gtcaatttca aatttttctg aaattttttt
    42961 tataaaaagg cattgttttg gattgttttc aatgctgacg acctgacgac aacctcttga
    43021 cacaaattca aaaccaatgc tgcctgttcc ggcaaacaaa tcgagtactt caatgtcttc
    43081 aaaatcaatt ctgttgttca atacatcaaa caaaccttct tttgcaaagt cggtagttgg
    43141 acgtgccgaa atgtcaaccg gcgcataaat atttcttcct ttgagtctac cgctgataat
    43201 tctcattata tattacttaa tcaattgttt cacattgttt tacgaatttt ctgacaaatc
    43261 cttgcagttt tgaatgctgt tccatgttgt aaagataaac cttacaattc tgcaagtcca
    43321 gattcaattc ttctatgatt ctcaaaagat aatacacaaa atcttcctgc gtttgatagt
    43381 cgaaactgtt taccaacaaa agtcggttgt gttgaaaagc taccacgtcc atgattttct
    43441 gccgtaccga tatacaaatg cattcttgca gcgaatgttt gtgaatgtgg ttttcaataa
    43501 aatgagaaat ggaatgctct attttgcaag cagggaaaag ctgtttgaaa acttccgaaa
    43561 tgtcctcagg caaagaaaaa atgttgatgg catcaaaagc ggagatttca ttgtataata
    43621 tggaagcatt tttttccttt tgatgagaaa agtaaagaag tttgtccatt tcttcttcct
    43681 taaaaatttc tttgggtaca atggtgtacg ttttggtttc ggcaatcaaa tgaattttgt
    43741 cgtaaagcag ttgggtttcg ggttcttgca gcagcaaagg aataatttgt tcctttgtca
    43801 tggagaaaag cggcatattc aatctttttg cagaaacaag ttttgtgttg tctctctcta
    43861 gaatagacaa agaaagtcca cccggtgaaa accggatgga caaactataa ggttgtatat
    43921 aggtatttgt agattctaag tcgccgcggg atgaatacat tttttcattt atctctgaaa
    43981 gacttacagg atttattccc agtttcctgc attattattg ggttcttcaa tagaaccgac
    44041 tttcagacct ggaaatttac ccagctttaa ggttcgatct atcaaattaa gtaattcctg
    44101 acgattcaga tcgtaaagat aactttcata gagagcactg gcttcaaaca atggaatgcg
    44161 aattcctgat atatttgtgt agttgttgtt aacttttaaa gtaaactcct cattttttga
    44221 atatggaata atacgcattt tgtcaatgtt gtctctattg tagtttggga aaacttcttc
    44281 caacaaatcg gtcagaatgg tatcacgcct aaaattttga agaccatatt ctgcaatttc
    44341 tttttgattt cccttgcgaa caatttcagc tgcttttgct tcggttaacc ctgcttccaa
    44401 ttgcttatcc gtaagaaaac cttctttcat cacaattttc tttttggctg ttttcaaaaa
    44461 atgaaacaag gtgtcaaagc tcgctgtgta acgtttgttt tgatctttaa attctatttg
    44521 cgctttacga atatcaacca aacactgaat aacatctttt tctctctgat tcttaatcct
    44581 ttcgaaacga ataggagtaa tgatactagc tacacaaaga tacgaaagta aaacaataga
    44641 aagagaaagc aaaattttaa tgactgtttt cataattgaa agattcaatt gttttttaaa
    44701 atacttattt tatggagaat aacgctacgc tattaacttt ttgcaaattt ataaaagaaa
    44761 attcaatact ccatctttct ttcaatattt tttaccttat ttttaatatc gcattcaagt
    44821 gcttactggt agaacgtatt ttgagtaaca attgatcccc ttccatacag aattaacttg
    44881 ctcatttttc atacacgcaa aaataacaat ttgtttttaa acctttggct ttttttcgga
    44941 ttaacaaaac atgtaatcgt tatatcggag ttacgttaaa ttaataatgc atttaatttc
    45001 aaattcaaca cactaatata aaaatttcga tgaaaatgga aatttttata aacattaata
    45061 aacacacatc caaacattaa aaaatattat ttatatttgg aatcaaattg ttggaaattt
    45121 ctttcatttt gaaaccttaa gtctttttgc aacatgaaga aaatgctttg tctgttgttg
    45181 attgcaagca cagtatccca aatttttcct caaatttctt caattattcc ggtttcttct
    45241 tctattgccg gaacctccgt agccaatacc ggcctgagat ccgaatttca aaatccggcc
    45301 atgttgggtt atgccaagaa cagtgaaatt ggtatgcaac atgaaaaccg atacgcactt
    45361 tccgagttgt caaccaacag ctttcaagct cttttgagta ccagtgcaat taacgcagat
    45421 ttgtcgtttt cttattttgg ttattcattg taccacgaaa tgatggccgg tttgggtttc
    45481 ggaagaaact tttccgacaa atttgcattg ggtgtgcggt tcaattatta catggcatac
    45541 ttttctgcat ccaattcgta tcgtggagcc ttgttgcccc aaattggatt gtcataccgg
    45601 ttttcgtcgt tattcagttt gggcttcagt acttttaatc cctttcagac aaacataaaa
    45661 accgatttta cacaaaaaag aattccttcc attttcagtt tggggacgga atattatttt
    45721 tctcccgatc tggtgtggct ggctcaaatc gacagggaaa tcagcagcaa ttatcgtttg
    45781 gcaacaggtt tcgaatacca aatgcttcag caagtttctc taaagttggg cgcttacgat
    45841 gccggttatc tggttccctg cggaggatgt ggactgaagt ggaaccgttt tttgctggat
    45901 attaattgcg aattgcatcc tttattggga ttgaactcct atgccggact tcgatatata
    45961 ttttctcgtt aattttttag tttaacgtcc ctatcatgaa gcgcattgtc atattagtat
    46021 gctgtttgct tttccttctc ttaaatgcag cctatgcaca agatgcgtcc acaactatcg
    46081 atcagacaat agccgatatt tttgaacagt ggtctgcaga atcggaagaa gttcccgatt
    46141 atcaaacatt ttacgaggaa ttggaatttc ttgccgaaaa tcccattaat ctgaatcata
    46201 cgtcgagaga agaattaagt aaaatgcttt ttctgtccga tgtgcaagtc gagaacattc
    46261 ttgcttatct ttatcagtat ggaccaatgg aaaatattta cgaactacag ctcatagatg
    46321 ggctggatat gacggacatc agacgaatgt tgccttttgt aatggtgaaa aaagaaaaag
    46381 cggtttctga aaaaatctat cgttatgacg ttttgaatta cgggaaaaac gagttgtaca
    46441 tgatgttcaa cagagatgcg gaaaccagac aaggctatca ggcatccttt tcatcaattg
    46501 gagattcaac aaatcagggg aaaaaatatg caggttcgcc cttgtatcat tctgccaaat
    46561 atcgttttca ttttaaggat agaattttgg ccggaatgac tttcgagaaa gatgccggtg
    46621 aatctttttt tggttctgcc cacaaaggtt acgattccta ttcgggttat cttcaaataa
    46681 atcgtttggg aattttcaaa accattgttt tgggtgattt cagagccaat tttggtcaag
    46741 gtttggttat gaagtccgat ttcagtatgg gaaaatcctc ctatgtcctt aatgttgtat
    46801 cgcgcaatga agggctgaaa aaattcagtt ctaccgacga atacaatttt ttcagaggag
    46861 gcggagccac aattcggcta cccagaaatt gggaaacttc cgttttttat tccaacaaaa
    46921 tgatagatgc cgatactgtc gggggcgtct tttcgagcat ttataaaaca ggttatcatc
    46981 gtaccgaaaa cgaattgaat aaaaaacata ccatttgcca acaggtagca ggcggaaata
    47041 tttcatggcg gttgctttgg ctgcaaaccg gaataacttt tgttcacacc aattttgatc
    47101 aggtacttgt gcctgaaaaa accacctaca atcgttttta tttttctggg aaagcccaaa
    47161 gcacagccgg atggaattat cgtgtccatt ggcagaaact caatcttttt ggcgaagcag
    47221 cagttacagg caactttcat cctgccttgt tgagtggatt cacgctgtcg cctctttcac
    47281 tagtcaatct ggtagctgtt taccgatatt attcacctga atacgatact ttttatgctt
    47341 tggcgctttc cgaaacttca cgcacaaaca atgaaagtgg tttgtatctt ggagccgaaa
    47401 taaatccggc aaaaaaatgg aaaatttctg cttatgccga cagttatcgg ttcccttggc
    47461 caaaatatgg aatcgatgct ccatcggcgg gaaaagatta tctttttcag accgattttt
    47521 ctgcccatcg gcgtttatcc atgttttggc gattcagaca cagcaacaaa ccggccaata
    47581 tttcacagga gaattcagtt atgcctgttg taaaagcggt caagaaaaat tcattgcgtt
    47641 atcagttgat gtattcgcag ggaaatttca gtttcagaac tgcggcagaa ggaaacatgg
    47701 tacagaaggg agaaaatgcc gattatactt acggattgac tgcctatcag gatttgactt
    47761 atgagttttc ttccttaccg ttgaagcttg atttcagata ccagtttttc gatgcggttg
    47821 cctacgaaaa ccgcttgtat gcctatgaaa aagatgtttt gtatgctttt tccatcccca
    47881 tgtattatgg aataggcagt cgttattatc ttaatctgaa atatgagctg aaccggcagt
    47941 ttactttatg gttcaaaata gctcaaaccg tatatgccga cgatcgtgaa gaaatcagta
    48001 gcggaaacga gaaaatttta ggcaacaaaa taacggatgt gaaatttctg ctgcgtttca
    48061 atttttaaaa aacaggattt tttctgctaa atatcgtttc agggttgata cgggctgata
    48121 cattttataa ctatcatttt ttatgcttgc tttacaataa tagtaatgca tgaactttat
    48181 aaacttttgt ggctattaac tatatattca aattttattg caataatcaa taaaaaatac
    48241 tgtactcttt taaaaatatc atttctttac tattttcaag ctcaaaattg ccaatttcct
    48301 attaaattgt aggcaatatc atttctattt cccttattcc attttctact tcttgtatat
    48361 ttactttcac tgtagagtct ggtaaaagag cttcaatttt ttcaggccat tcgatgaaac
    48421 aaatttttcc gctataaaaa taatcttcag ctcctaagtc gatggcttcc tgtaacttat
    48481 taatacggta gcaatcaaaa tggtacaaaa ctcgtccatc tgctgcttca tattcattaa
    48541 taatggaaaa agtaggacta ttcactgtat ctttcactcc aagaatttca caaacagctt
    48601 taataaaggt cgtcttaccg gctcccatac ttccataaaa agcaaaaacc gagtatctat
    48661 caaataaatt aataaatttt cgcgctggct gctgaatatt attcaagtca aatatttctg
    48721 tcatgttcaa tttttaatct ttaaagttat tacaaataaa aatttattga ccaaaataca
    48781 ctcattttga aatttgcgat tctggagcac tcaaatcttt gtgaaaatac ccataaacta
    48841 ttgatgctct gtgatttccg acaacttttt gaatatcttc cagatttgca ttctggagtc
    48901 tttttatact tttaaaatga tttatcagtt ctcttttggt cttatcccct attcctttaa
    48961 tattatctag ttcagatgtg gtttgatttt tacttctttt ctcgcgatga aatttaatgg
    49021 caaagcgatg cacttcttct tgcattgtag ccaacaattt gaaaagaggt tcattgggtg
    49081 tcatgccaat attttttgga ggaaagccga aaagaatttc gcgagtatgg tgtttactgt
    49141 ctttggcaag tccaataatg ggaattctta aattaagctc atcttcgaca acttccctga
    49201 tcacttccat ttgtccaatt ccaccatcag ctatgatgag atcgggcaat tctccgtttt
    49261 ctgctatcat ccgagagtaa cgtcttttta ctacttcttt catggaagca tagtcatcgg
    49321 gacctacaac tgtcttaatg ttgtattttc gataatcttt ttttgaaggt ctcccctttt
    49381 tgaaaaccac gcaggctgct actgcatctt tcccctgaat attggaatta tcaaaacatt
    49441 caatagttat tggtaatttc tcaagatgca atttctcctg aatttctttt aatagctgca
    49501 tacttctttg atcaggattt aatttttcag cttgcttaag tttctcaaac ttatattgca
    49561 gcacattttg ctgagccaaa tccaagagtt ttttccggtc tcctctttgc ggaataacaa
    49621 tgctaacgcc attcggctta taattgattt caaaaggtac gacaatttct ttggagttac
    49681 tttttaatct ctctcttaat tctactatgg ccatagcaag taaatcttcc ttttcctcgt
    49741 caacatgttt ttggtactcg atggtatatc cctgaattat tgaacctttt gaaattctca
    49801 agatattaat ataagcagca ttttctgttt cttcataacc aaacacatct gtgttttcta
    49861 ccactgaatt ggcaatgatc gactttgatt tgtaattttc aatcaaatca tatttttgtt
    49921 tgatttgata tgcttcctca aatttgtaag cagcagaaag cttttgcatt tcttcccaaa
    49981 gcattttgct tatttcgttc gcattaccct caataatttg cttaatttcg tttatgtatt
    50041 tccgataaat ttcagcagat tcttttcctt cacacggccc attacactta tggatatgat
    50101 attgaaggca aactttaaat tttccagctt tgatgttttc atcggtcaac tgaagtttgc
    50161 aagttcggat gggataaaga tcatgtatta tctctaaaag agaattgatc gtgtaactcg
    50221 aactataggg tccaaaatat aaggaaccat ttttaataat atttcttgtt ttgaatactc
    50281 tgggaaattc ttcattcgtt atacaaatgc ttggataggt ttttccatcc ttcaataaaa
    50341 cattgtaacg cggttgaaat ttttttatta aattgttttc caacaacaag gcatcttctt
    50401 ctgtgtcaac cacgatatat ttaagagatg agatatttct taccaatact gccgttttac
    50461 gtgattcgtg cgttttggta aaataggagg aaacacgctt ctttaaattt ttagccttcc
    50521 caacataaat aatctcctcc ttttcattga agtattggta aacacccggc ttttcgggga
    50581 gagcatcaat ttgctgcttt aattgaggat gcaaattcat aatacttttg aatgtttcac
    50641 gtgaaacaaa gttagaaaaa attctttaat ttcccgattg ttccacgtgg aactataaaa
    50701 ttatcgtccc attagaacta aaagtatatt aatgtcactt ggagaaattc ctggtatcct
    50761 actagcttga ccaatagttt caggatcaat ttttgacaat ttttgtcgtg cctccgttga
    50821 taaggatgaa atagtatgat aatcaaattt tcctttaata ttgatgtgct ctaacctgtg
    50881 aaacttttct gcaatagatt tttcgcgatc tatatatcct ttatatttca aagaaatttc
    50941 tgctgcttcg actatttctt caactctatc tcctattgat tgaatttttg attttaaagc
    51001 aggtacaatt tcagataatt ccataataga aatctgagga cgaagaatca aatcaacaag
    51061 tttacaacca tgagttaact ctgaagaatt ttttgacact aaaaaaccat tcacttgtgc
    51121 aggtttgatt gagaaatttt caacaaaagt ttttaaagat tctatagcct cgtatttgaa
    51181 agaatattga tttattcgat tctttgtaac taatccaatt tcataaccct tgggtgttaa
    51241 ccgtaagtct gcatcatctt gacgtaaaag gatacgatat tcagcacgtg aagtaaacat
    51301 acgataaggt tcgtcaactc ccttagtaac caaatcgtca attaaaactc caatataggc
    51361 ttcatcacga tccaataaaa attccttccc tccatgacaa tttatatgcg cattgatacc
    51421 cgcaattaat ccttgtccgg cagcttcttc gtaaccggta gttccattga cctgtcccgc
    51481 aaaaaataaa tttttaatgc gtttggtttc gagtgtatgc tttaattgcg taggatcaaa
    51541 aaaatcgtat tcaattgcat agccgggacg ataaagttcg gctttttcaa gacccggaat
    51601 cgtatgtata gcttgaattt gaacttgaac aggaagagaa gaagaaaaac cgtttaaata
    51661 gtactcttgt gaatcaactc cctctggttc taaaaacagc tgatgagaag atttatcggc
    51721 aaaagtaaca attttagttt caatacttgg acaatatctc ggaccaatac tttttatttg
    51781 accgttataa agtggagatt cgggcaaccc ttgtcgtaaa atgtcatgag ttaattcatt
    51841 cgtataagtt atccaacaac tcatttgttt caattctcgt gttacttcag tgagaaaaga
    51901 aaatttatga aaatcgtttt ctcccggttg ttcctttagc acagaaaaat cgattgttct
    51961 tccatcgata cgtgctggag ttccagtttt cattcggccg gtagtgaaac ccaatgattt
    52021 taattgctct gtaataccaa aagaagcagg ctcagaaatt ctacccccga ttagttgatt
    52081 tttaccgata tgaataaggc cgctcaaaaa tgtaccggct gttaatacaa cacatttagc
    52141 tgagatttct attcccaaag cagttaaaac acctgtaact tgcccatttt cgataagaat
    52201 ttgctttact gtatcttgcc aaatataaag attaggggta ttggttagcg tctgaatcca
    52261 ctgaagaata aattgatgtc tatcactttg ggagcgagga ctccacattg ccggaccttt
    52321 agaacgatta agcatacgaa attgaatggc agagcgatcg gtaacaactc ccatttgacc
    52381 gccaagtgcg tctatctctc taacaatctg accttttgct atgccgccta ctgcaggatt
    52441 acaactcatc tgaccaattt tattcatatc catagtgatg agcagcgttt tagatcccat
    52501 attggcagcg gcacaagcag cttcgtttcc tgcatgaccg ccaccaatta caattatatc
    52561 atatttaaaa tccatatcga gtaattaaaa taatatccga gcaaaaaata acaaaagaat
    52621 aaagtattag ttcaaaaaat aggataaaaa aaatatcagg caaataaagc tttgccaatt
    52681 atggtggaaa tttgctccag ataataaata tcagcttcat caagaacacc aaatttttcg
    52741 ctatctatat caagaacgcc aaccacttct gaagaagaat tgtacaaagg aataactatt
    52801 tcggaaaccg aaatactgct acacgctata tgtccagaaa aatcattaac gtcaggaaca
    52861 agaagactgc gtttttcctt ccatgctgtt ccgcaaacac ctttaccaaa ggcaattcga
    52921 gtacaggcaa caggaccttg aaacggccca agcacaagaa aatcttcctt aaccaaataa
    52981 aagccaaccc accaccattt aaaagtctga tgaaaaacgg ccgataaatt agcaagattt
    53041 gctacaatat ccggttcatc agctataaga gcaggaatct gttgtaataa aatctcatat
    53101 ttggattttt tatccagaga agaactaata aagaaatttt cagacatcac gaaaaatttt
    53161 tttgcaaaat tacgctaaaa aaatcatttt taaatggatt aaataggaat caaacaaaca
    53221 aagaaaaagt caatttaaca ttcatatcta aaaatttcga aataattttc ttatctaaaa
    53281 ttttatcaat tactttgcta caaatagaag agaacgtttt gagatataaa aaaattatta
    53341 ttaaaatgta aacaaaaaaa tcatgagaaa attagagttt aaatttttcc cttttgtact
    53401 actgatttgt ctgaatacat caatgtttgc acaacaagtg cagttccaac ctttgccgat
    53461 agacccaaaa gtcaaatatg gaaaattgga aaacggatta acttattaca ttcgttcaaa
    53521 tcaacaacca aaaaacagag cagattttta tatcgttcaa aatgtgggtg caatactcga
    53581 aaacgatgat caaaatggat tagcgcactt tttggaacac atggcattta atggcacaaa
    53641 aaatttccct gggaaaaaaa ttattgaata cttcgaaagt gtaggagtaa aatttggagc
    53701 taacatcaat gcctatacgt ctcttgatga aacggtttat catttatcga atgttccaac
    53761 caacagggaa agtataatag acagtgcttt acttgtgttg cacgattggt caaatgcaat
    53821 ttcgctcgaa gaaagtgaaa ttgaagcgga aagaggagtt attctcgagg agtggagaca
    53881 gggagcaagt gcggatcgaa gaatgtggaa agaatcaaac aaacaaaaat tccccggctc
    53941 acaatatgcc aaacgtgatg taattggaga tactgcggtt ataaaacatt ttacgccgga
    54001 agcattacgg gattattata aaaaatggta tcgacccgac cagcaggcaa tcattgtggt
    54061 aggagacgta gatgtaaaca aaattgaaga aaaaattatc gaattgtttt ccaatatcaa
    54121 aaaaccggaa aacgcacctg aaagaccaat ttatccaata tacgacaaca aggaaccaat
    54181 aatctctata gttactgacc cggaagccag aatgacaaga gtggaattaa tttataaaca
    54241 cgacaagtta ccggcagaaa ttaaactgtc cataaacggc tatctggtga atgtgataaa
    54301 ttcgttaatt tcaaatatga tgcaaaacag atttgaagaa ataaccatgc agcccgatgc
    54361 accttttgta gccggatatg caacgtatgg agaactggtc aaatcaaaag atgcattcta
    54421 tctggtagta gtaccaaaag aaggctcaga aactgaagga attaaaagcg ttttaattga
    54481 agcagaaaaa gttaaacgtt ttggattcaa tcattcggaa ttggaaagag caaaagccga
    54541 tttggcaaaa atgatagaaa aacaatataa tgaaaaagat aatcagaaaa atgaaagtta
    54601 tgtgagagaa tatatggaac attttctgaa tcaagatccc ataccgggta tagaatggga
    54661 atacgaaaca attaaaatga ttttgccaca aataaatgcc gatatggtga atcaacttgc
    54721 aaaatcttac gtaaccgaca caaatatgat tgtttcgata atggcgccac aaaaacctac
    54781 ggtagtcctt ccaagtaaag aagaaatcag gcaaataatt gaagaagtca aaaaaacgga
    54841 gctaactgcc aaattggacg aaaaaataga taaaccttta attgaaaaaa tgccaaaacc
    54901 cggtaaaata aaaaaagtat caaaaaatga tattcttgga acaacagaat ggatattgag
    54961 caatggtgca aaagttatat taaaaccaac cacattcaaa aaagatgaaa tattgttcaa
    55021 tgcctacagc ataggaggcc tttctaaagt tgaaaatatt gaagatttac cttcggctga
    55081 atttgcaaca gaagttgtaa gcaacaacgg cttgtccgat ttcaatcaaa tagaattgaa
    55141 aaaattactt tcgggaaaaa ttgcttcggt cagtccctat atcgacaatt atgaagaagg
    55201 cttctcagga aattcttccg tatccgattt tgaaacaatg atacaacttc tatatttaca
    55261 ttttactgct ttaaggaaag atgataacgc ctttacggct ctgatgaaca attataaagc
    55321 cttcctggca aacagcgaga ataatcccga caaggctttt tccgattctg ttaaattaat
    55381 gctttccaac cacagtcccc gtacggtgct tttaaatatg aaaacattgg aaaaagtcaa
    55441 ccaggataaa gcattggaaa tatttgctga gcgattttct gacccggccg actttacatt
    55501 tgtgttcaca gggaatgttg atccaaatga taaaacggta gtcaatgcaa ttaatacttt
    55561 cattggcgga attaaatcga aaggcaaaaa agaatcgttt actgatgata atgtaagact
    55621 tccagtggga agagccaaca accatttttc aagagaaatg caagtcaaaa aggtttcaaa
    55681 ctgcattgtt tacagcggaa atttgaattt taacatgaat aatcgaacaa acatgaatgc
    55741 tataggaaat attttaaata ttcgctatct tgaaagtgtt cgagaaaaag aaggtggatc
    55801 atatggcgta ggcgttgctg ccaatttgag aaatcgaccg attgaacagg caatgctgtt
    55861 cattcaattc gatacgaaca ttgaaaaaca aaacaaattg atggatatca tacattccga
    55921 aatcgacacc ataatcagca aagggccaaa agcagaagac ttacaaaagg ttaaagaaac
    55981 aatgatgaag aaatacgagg aagatctgca agaaaataaa tggtggcaaa gtgcaataaa
    56041 aaattattat ttcaataaat tgaatgtagt taacgattat cttccatcgg ttcaagcact
    56101 gacaagtgaa agtattcaaa aaacactgaa aactatcgtg gaacagggaa atattatgga
    56161 aatagtcatg aaaccaaaag aataaaatca attgcagctc aataaaaaaa tttctctgca
    56221 agaattcagt aaaaaaaaat cttacagaga aattttttat ttatacaact gctagaatta
    56281 ccaaatgcag gcgcgctttt ctacaggcaa atacaattta tctccttcct taattccaaa
    56341 agcatcatac catgcctgaa tatgaggcaa agccccattt accctccaac gacccaaagc
    56401 atgcggatca gactttgtca aaacaagaat ttcttcggga cgtatgttgc aagcccaaac
    56461 attagcataa gacaaaaaga aacgctgttc gggagtgaaa ccatccaaaa ttttcgatgg
    56521 ctggcctttg gttgaatttt tataagcctg aaatgaaact tgtaaacctc catgatcagc
    56581 aatgttctct cctaaagtaa attttccatt agcatggaca ccgggagcaa cttcaatgga
    56641 atcgaaataa tcggccaaaa cttttgcgcg ggcatcaaac tgcttcttat cttcttcagt
    56701 ccaccagttt tttaaatttc catccttatc gaactgacaa ccctgatcat caaaaccatg
    56761 cgtcatttca tgacctatca caacgccaat ggctccataa ttaacagcat catcggcatg
    56821 gacatcaaaa aatggaggtt gtaaaatacc ggccgggaaa caaatttcgt ttgtagaagg
    56881 gttgtaataa gcattaacag tttgaggagt catgagccat tcggacttat caacagggtt
    56941 accggctctt gcaaacttgt aagcatactc aaagtgatga gctcgtttaa cattttcata
    57001 ataagaaaca tttttagaaa tttgcagcgc atcataactc ctccacttat cgggataacc
    57061 aatttttaca taaaaagttc ccaatttttc caaagctttt gcttttgtcg aatcgcccat
    57121 ccatgttaaa ttttgaatac gctgacccaa tgattcctga agattgtgca caagctttaa
    57181 catactttct ttagcttcgg gaggaaaata tttctttacg tacatttgac cgactgcttc
    57241 gcccaaaaaa ttgtcaatac ttctcaccga tcgtttccaa cgaggcgaag gttctttttt
    57301 cccagacaaa gtttttccgt aaaactcaaa attctgattg taaatactgt cgcttaaata
    57361 agcagcagca ttatcaataa ccttccattg aagataagcg atctgttgat ctaaaggaac
    57421 ggtagtaaga atattcaaga cttcagtcaa aaattctttt tgagaaacat ttactgaatc
    57481 aacagcagga attcccaaag tgtgaagaaa ttcattccaa gaaatagtag aaaattcttt
    57541 acttaaatcc tgaatggaca ttttatgata attggcataa ggatcgcgaa gttttacacg
    57601 atcataagac gctttggcta aacgagtttc aatatacaaa acagcatcgc gatttttctg
    57661 agcatcggca gaattgaagc caaataactc aaacatttta gctacatgtt gtttatactt
    57721 ttcaagaatc tctttggaat gcgtgtcctg atccaagtaa taatctcttt ctcccaaact
    57781 tataccgctt tgataggttt gtgtcaaata taccgaacta ttcattggat cagcatcaac
    57841 gtaaaccgaa aaataaacgt cgttgccatt cagcagtagc tccggaagaa ttttaatcaa
    57901 ctcggtgttg gtttttacac cagccaattt tttcaattca gtttgcaccg gttgatagcc
    57961 ttccctattt aaacggtcgc tatccattgc caattggtac aaatcaccaa ttttttgctc
    58021 cacagtaccc tcaacatgcg atttagaagc aatttcttct atcaattcac gaagctgaga
    58081 agtattttcc tccgccagct tgtcaaaagt tccgtaacgt gcatattcac cttttaaagg
    58141 attttttttc atccatccgc cacaggcaaa ttgataaaaa tcatcaccag gacgagcagt
    58201 agtgtccaaa tttgtcaagt caatgccaga aatttttttt tctgatttac atccattcat
    58261 cataagcatc ataaaaatta aaaatggtat ccccaacgag agaaaaccaa acatacgtga
    58321 tttaataata aaattcataa acagtagaat ttagaaaata taattattca aaaaaactaa
    58381 ttgcaaaaag tttcatgtac agaatcgata atatcgaaaa gttcacgcaa tgtatcggca
    58441 cgaagcatgg caatacgcgt acttttaaaa tttggaatat tcttaaaaat agaagttgct
    58501 gccaaatgtc gacgagaatg aacaattccc tttaaatgta tatcataaac attttctata
    58561 ggtgtttgtt catcaatatc caacctgcga acggcagata aaatatagtt ttttaaaata
    58621 gctttcttat cttcaaaaga aagaggcgaa tattcaaagt cgtcatcaag gacatgcttc
    58681 atttcagaaa aaatccatgg ctgaccaata gcagctcgtc caatcataat tgcatcaacg
    58741 ccataacgat tgaaaaaatc ttttgcctgc tctggtctgg taatatcacc atttccaata
    58801 ataggaattt taattcttga attgtttttg acttcaccaa tcagcgtcca gtctgcctcg
    58861 cctttgtaca tttgttgacg cgttcgacca tgaatagtga gcgcctgaat accgcaatct
    58921 tgcaacattt cagccaattc aactataatc ttactgctgt catcccaacc cagacgcgtt
    58981 tttaccgtaa cgggaacgtt tacagctttc accacaaatt gagtaatttt caacattctt
    59041 ggaatatctt tcaacaaccc agctcctgcc ccttttccgg cgactttttt taccggacat
    59101 ccaaaattaa tgtctataat atcggggtga atcatttcaa ctttctgagc cgattcagcc
    59161 ataacctccg ggtcggtacc gtaaatttga attgctacag gtctttcctc ttctaacaca
    59221 ttgatttttt gtaaagttcg aggaatatta cgattcaaag cctcagacga aacaaattca
    59281 gaataaacca tgtcggctcc aaaatgacgg cataataagc gaaaatcagc atccgtaaca
    59341 tcttccatcg gagccaaaaa caaaggtcgt gaaggaaatt ctatgtttga aattttcatt
    59401 tttatgcaaa aaagagtaca aatttagaaa aaaaattgga ttgatgattt ctaaaatgga
    59461 attaaaaaac ccatacgagt gaaaaaagtt gctccataaa tagaaataaa ccaagtaaaa
    59521 gattgagaac gtgtagtgag gagttgaata gaaaaattaa aataaaaggg ttaattttgc
    59581 agtaagaaat tagaaaaaaa gcaagattca ggagaaaatc aaacaatgga aaattatata
    59641 aacaaaaccg aaacttatcc aaccgaacgc agcaaaatag aaattatggc accggcagga
    59701 tcatgggaaa gtttggctgc agccataaaa gctggagcag actcagtata tttcggtatt
    59761 ggaaaattaa atatgcgtag caaatcgagc agtaatttta gcgttgacga tttgaaaaaa
    59821 attgttgaaa tatgcacaga aaacaacgtg aaaagttact tgaccgtgaa tacaatttta
    59881 tacgacaatg atttgatttc gatgcgcgaa attatcgaaa cagcaaaaga agccggtatt
    59941 tcagccatta ttgcctcaga cgttgccgcc atgatgtatg ccaatagcct gaacgttgaa
    60001 gtgcatttgt ctacccaatt gaatatttcc aacatcgaag cattgaaatt ttatgcacag
    60061 tatgcagatg ttgtagtgtt agcaagagaa ttaaacttaa aacaggtttc ggaaattttc
    60121 agcgctattc aaaccaaaaa tatttgcggt agaaacggaa aactgattcg gattgaaatg
    60181 ttttgtcatg gagcgctttg catggccgta tcaggaaaat gctatctcag ccttcacgaa
    60241 aaaaatctat ctgctaatcg cggcgaatgc aatcagattt gtcgaagagg ctatctggta
    60301 aaagacaaag attcggaaat agagctagaa atcgacaatg aatacatcat gtcgccaaaa
    60361 gatttgaaaa caatagaatt tgttgacgaa atgctgaaag ccggagtcag tgtatttaaa
    60421 atagaaggac gtgctcgcgg agccgaatat gtgaaaacag tcgtttcatg ttataaagaa
    60481 gcaatcaatg cctgctatga aggaacattc agcaaagaaa agataaacga ctggaaccgc
    60541 agattaagta aagtttttaa cagaggattc tggaatggat attatttagg acaaagactt
    60601 ggcgaatgga gtaaaaacta tggttctgca gcaacccaca aaaaaattta catcggaaaa
    60661 gttaccaatt atttcaataa aattggcgta gcagaatttc tgttggaagc tcaaagttta
    60721 tcggtaggcg acgaaatatt gattactggc gaaacaacgg gtgcatacga agacattgtc
    60781 aacgaaataa gagtagattt gcaacccgtt gagaaagtgg aaaaaggaac ctacttctcc
    60841 atgaaaacca atgaattggt tcggcggaac gataaacttt tcaaaattgt gcctaccgaa
    60901 cacggtaaac aagaaggaaa ataaatattt ttttggtaaa taaaaaaata tacttttcag
    60961 aaaatttctt ctcttggaaa tttatcttcc aacttgctaa aaaggaatga aataaccaca
    61021 ccaacagcaa gaaaaccaat acacaaaaga aaagcaccca caaaacttag tcctgcaacc
    61081 gacaattgtt cagaccgaaa agcgggaaaa acaaccgtcg gaaaaattgc caatgatgac
    61141 cctactacca ttcccaaaat aaaatgatac atttcgggat aaaactttct gaacaaataa
    61201 gctgccagct ttgcaaaaag taaaacacaa accgcaaaac caacaagaag aggaataatc
    61261 acggcaaaat caaaatcctt aattccggtt gccattttat cgtataatcc gaaataaatc
    61321 aaaaagttgg aaggactcat accgggaaca atcaccccca aaccaatcag cgcaccggaa
    61381 cccaaccaaa ccggaatatt gggagcaact tccgtcaact gctgaccacc cagcaacatt
    61441 aacccaaaaa taaaaaacgt agaaaaaata aaaatgataa tatcttttgt tcttctcccc
    61501 tgttttcctg ctgttctata aagcgatgga aaagttccgc ctacaaatcc cacaaaaagg
    61561 caaataaatt gagcggcata attaccaaaa gccttctcca ccaaaataga aaaaagcacg
    61621 attccgattg ccccgccaat tactaccgga ataaaataaa aaacattctg aacgaaatcc
    61681 tttcgcaaat cggacaaaaa tttgataatt ctgtcataaa ttcccaaaat tactgcaagc
    61741 actccgcccg acaaaccggg taaaataaaa ccaataccca caattatacc ctttatcaat
    61801 ctaataaacc agtcaacaac aacatttgat tgagtatttt tcgagccgga agtattcact
    61861 ttgtcagtta cttgagtcat aaaaaaattt ttaaaaattt cgcttgcaaa gttacacttt
    61921 tttcgttcaa ttggagaaaa gaaaattttg gatagaaaaa aacagaaatg aaatgttaga
    61981 tgtaaagata taaaatatac tttaacaaat gctttatcaa aaaaacatgg aaatacccat
    62041 caaaaaaata aaaacaataa aaaatggcgt gtaaaacttt tccaattttt tttcgtcaat
    62101 tttctttccc aaaattgggc ccaaaatagc tccggtaatt gttcccgcag ccaataatcc
    62161 taccaaatac caattgagat gaccaaaggc ggtatgtgtt atgactcctg aaagagcaat
    62221 aaaaaaaagt accataactg acgttcccac cacctgcacg gaactcaact tcatattgta
    62281 aagtccggct aaaatgggtg gagttccact tactcccagc aaaccggaca taattcctcc
    62341 aaaaaaacca aaacttaaac ccttaaaatt tttgaaacta aaaatttttt tcttttttgc
    62401 tatgtttaca gttttatctt cttctactaa aataatattt cccttattga aagatttttt
    62461 tgaacgaaaa tttttaaaaa tcatttgcac agccataaat atcatgaaca aaccaaatac
    62521 aatttttaaa acattcaagg ataaaagtcc caataaaaaa gagcccgcta taactccaat
    62581 aattccaccg gctgaaagaa tcaatcctac gtttatattt acatttccag ccttaaaatg
    62641 gcttatagag gctgcaaaag tagttggtat gatggtggca agtgaaacag aaactgcctg
    62701 attcatagga atggaaaata atccagtcaa aatggcaata taaaaaattc ctcctccgcc
    62761 tcccatcaaa gaaattagta ttccaatcaa taaaccggct aatggcaaaa aataaatagg
    62821 taaaatcatt attaaaaatt agttatacaa ttttttttca acttaattcc aacattcgca
    62881 aaataggttt tacagctttt tgtcggattt cctcctcaac caaaatctct ggactttcat
    62941 ttaaaagaca ttgatatacc ttttcaagcg tatttaaacg catgaaattg cattcgttgc
    63001 acgcacacgt gctgtcgttt ggtggaactg gaataaattg cttgtcggga tttttctttt
    63061 gcatttcgtg caaaataccc gattctgttg caaccaaaaa aaatttttta tcggatgatg
    63121 tagcaaaatt caataaagcc tgcgtgctgc caataaaatc ggccagcatt aaaagtggtt
    63181 tcgtacattc gggatgagcc aaaaccaatg cttcgggata tttctttttc aattccacaa
    63241 gtttttgtac cgaaaactgc tcgtgtacat gacaagctcc gttccacaaa agcatttgtc
    63301 ggccggttat gctgttgatg taatttccca aatttttatc tggtgcaaag ataattttct
    63361 cttcttttgg aaaagattcc acaatttttt tggcgttggt tgaagttact accacatcag
    63421 ttaaagcttt aacagcagcc gatgtattga cataagaaat aaccgtataa tcgggatgat
    63481 tttttacaaa ttcttcaaag gcgtctgccg gacaactgtc agcaagcgaa caaccggcat
    63541 tcatatcagg tatcagcact tttttttgcg gattaagaat ttttgctgtt tctcccataa
    63601 aatgaacccc acaaagaaca ataatatcgg catccgtttg cgctgcccac tgtgccaatg
    63661 ccaaactgtc tccaacatgg tcggcaatat cctgaatatc tggctcttga taaaaatgag
    63721 ccataatcac agcattttta tcttttcgaa gtttatttat tttttctatg agctcttttt
    63781 tttccatagc tttgttgtta tcaaatatat atcatattaa tattttattc aattaaataa
    63841 ataatatgaa tatgataata gggtgttaat ttggttaata tttattagaa aaaattcttg
    63901 cttaattaat tattaaattt tatagtatat ttgtgcactg gtgcgggtaa tcaaatagtt
    63961 tgaaaaacag aattttataa atgttatcaa caggttgttt ataactacaa agttaataag
    64021 tttttgaatt gtttcggtga ataaatctat taaatatggt tgaaaaatta tatgaaaata
    64081 tttaactcta aattttcaaa atccaaaaca attgatatgg aaaattgttt ttggtctttc
    64141 aataaaaatt ttcaaatgat tataaattag ttttcaacaa catttcagcg tagaatgttg
    64201 ataaataaaa attctatgaa ctgtaaagaa ataattgaaa atgaatatat tgctttaaaa
    64261 atgaattaaa acttttgagt agtgaaaatt ttttcaactt cagtagttcg tcgtttggac
    64321 gaatatacca taaaaaaaga gcccatcgca tccattgatt taatggaaag agcagctgtt
    64381 gggctttgta aaaagttttt agaaatattt ccttctcccc tacccgtttg tatttttgca
    64441 gggcccggaa acaatggagg tgatgcgctg gctttagcaa gaatgctgct tcaaaaaaat
    64501 tattatgtta ctgtatattt gattcataca ggaaaacttt ctgcagattg tgaagccaat
    64561 aaaatcagac tgatagaaaa atttcccggc tgtttgcatg atttttctac tgacttttat
    64621 cctgtgaatt tttcggaaaa taccgtcata atagatggcc tttttggtac gggtttgtcc
    64681 cgaccattga gcggaattta ttcggaagcg gttgggtgga taaataattc tggaaaaacc
    64741 gttgtatcca tagatattcc ttccggattg gagggtgaag aaaataaaga tttatcacaa
    64801 cccatagtaa aagccgatta tacattttcg ttgcaatttc caaagctttc ttttttcttt
    64861 gcagaaaacg aatcttttgt ggggaaatgg gaaataatag aaattgatat tcatcctgaa
    64921 gccattagac gagaaccaac cgatttttat tttttagaaa aaaaagatgt aattccgttt
    64981 ttaccggaaa gaaaaaa
//
LOCUS       DAOSCH010000003        58957 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_406_length_58957_cov_5.962005, whole genome shotgun sequence.
ACCESSION   DAOSCH010000003 DAOSCH010000000
VERSION     DAOSCH010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 58957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58957
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_406_length_58957_cov_5.962005"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..2983)
                     /locus_tag="PLZ54_00530"
     CDS             complement(137..2983)
                     /locus_tag="PLZ54_00530"
                     /inference="COORDINATES: protein motif:HMM:NF019136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triple tyrosine motif-containing protein"
                     /protein_id="HOJ65458.1"
                     /translation="MKKIHLLFIIFCACAANAQEFIPLVTQFSKNDYRAANQNWAVAQ
                     GLDGILYFGNNEGLLEFDGSLWKLHKIPGNGLVRSVMIDKKNRIYIGAFEEFGYFERD
                     RFGQLNYTSLSALLKNYKMQNDEVWNIFDDQGTIFFQSFTSYFTFKNGEVKGFRTDFS
                     FLFFNLYQNTLYTHTFQKDFCYFDRQQNTFVPVPNNIMKSPVIKILPFDDKHALMVTI
                     SDGLYLYDRDKFTRFATEVDDVLKKAQINRAVISPNGLIVIGTILNGAVAIDKSGRKI
                     WAISSSNVLQNNTVLGITFDRDNNIWLALDKGIAFIHQNSSFRCIQSFYPAIGAIYSV
                     SYDPPHIYIATNQGLYRALLEHHSIRNLSIDPKIKGQVWDVSSFDGQQFCGNNEETFE
                     VTPTRSIPVSPVKGGMCFAHGNIHGVEVLVQGTYTQLCIYKKENGKWKFSHAVEDFLH
                     PIRYIEIDYTGTIWASHLHRGLFAIQLTPDLRKIEHINIYKSLDQQHLNTINVFSVNN
                     RVVFTDYNLFYTFDDIRKKIVPFDELNKALGNFSNSYRICYFKGNLYWFIRPGEAALV
                     EIKPGQIKLVDMVQYADFLHQTVDDYQNIIPISENECIFTLENGLALYNYTQKLIPQY
                     SIQLKLKSIQISDAANKKLNFLPLEATSLPTIPYSRNNVVFSVFYPEYAQRNNVMYRF
                     KLEGLDVVWRDPSSSPSIEYKYLPYRKYVFKAEAISESGQILSETNFAFEVQPPFYLS
                     TPAKIIYTLAVLFLLWGIYWYVQRIIHRKKEKVRIEQEDIRRKEIEKREQQIMTLEKE
                     KLESELTLKSKELATSTMTLIRKNEVLATIKEEIMQQKAALGSQYPNKYYEKIIRLLD
                     ENLSSEDDWAIFQANFDRIHENFFRHLHERYPELTPNDLRFCAYLRLNLSSKDIAHLM
                     NISLKGVEMGRARIRKKIGLPSTKSLTEFMIEFK"
     gene            complement(3382..5091)
                     /locus_tag="PLZ54_00535"
     CDS             complement(3382..5091)
                     /locus_tag="PLZ54_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ65459.1"
                     /translation="MMNFYNDNPALKFQLSHPLMQKIVTLKERNFADKDKYDYASRDF
                     EDAIDNYEKILEIIGEICGEVIAPNAEEVDHTGPQVVNNRVVYAPGTQQNHEVLTQAS
                     LYGIGLPRQYGGLNFSMVPYVMAAEMVSRADAGFANIWGLQDCAETINEFADEDLKRE
                     FLPRVCQGQTCSMDLTEPDAGSDLQAVQLKATFNEKDGIWYLNGVKRFITNGDAHIKL
                     VLARSEEGTTDGRGLSLFLVDNKHDDTVKVRRIENKLGIKGSPTCELVFSNTPAKLIG
                     SRRLGLIKYVMSLMNSARLGVGAQSVGLSASAYNEALKYAKERQQFGKAIIEFPAVFE
                     MISLIKAKLDASRALLYETTRFVDMYKAYEAIEKERRLEPEEKAEYKEFQRLADAFTP
                     LLKMFASEYANQNAFDCVQIHGGSGFMKDYACERLYRDARILTIYEGTSQLQVVAAIR
                     HVTTGTYLQQIREYEKLPINPEFHSFRKRLMIMTNLYENSVSRVIETKNQEYIDFQAR
                     RLVEMAGHIIMGYLLILDATRNDMFRKSAEVYINYGEVETRKHATFIKTFDMENIDIY
                     KVV"
     gene            complement(5205..6224)
                     /locus_tag="PLZ54_00540"
     CDS             complement(5205..6224)
                     /locus_tag="PLZ54_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOJ65460.1"
                     /translation="MNNLFVYLEIEDGIVKDVSLELLTKGRSLAKQLNCKLEAVAIGY
                     QLENIPKQVFPYGVEVLHLADDKRFVPYTTQPHASVLIHLFKEEKPQIALLGATSLGR
                     DLGPRVSSALFSGLTADCTSLEIGDYEDKKAGKVYKNLLYQIRPAFGGNIVATIVNPD
                     CRPQMATVREGVMKKEIFDPKHTGEIRRLEVSKYVSDQDFVIEVIERHIEKTKIDIKS
                     APIIVSGGYGVGSAENFKLLYELAEVLGGEVGASRAAVDAGFAEHERQIGQTGTTVRP
                     KLYIACGISGQIQHIAGMQESAMIIAINSDPNAPINKIADYVISGRVEDVLPKMIKYY
                     KKNTK"
     gene            complement(6314..7186)
                     /locus_tag="PLZ54_00545"
     CDS             complement(6314..7186)
                     /locus_tag="PLZ54_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOJ65461.1"
                     /translation="MSFNIVVLAKQVPDTRNVGKDAMKADGTVNRAALPAIFNPEDLN
                     ALEQALRIKEKVPGTVVHLLTMGPVRAAEIIRESLYRGADDGFLLSGREFAGSDTLAT
                     SYALSCAIRKLGKIDLILCGRQAIDGDTAQVGPQVAEKLGLPQITYAEDIIEITKKNI
                     TVKRRLERGVEIVKGPLPILVTVNGTAAPCRPRNAKLTLKYKHAATPSEKQESGSDYT
                     DLYDAHPYLNITEWGANDIEHDPKWLGLSGSPTKVKKIDNIVFTAKESKRLTGSDFEI
                     ESLMKELIANHTIG"
     gene            complement(7835..10474)
                     /locus_tag="PLZ54_00550"
     CDS             complement(7835..10474)
                     /locus_tag="PLZ54_00550"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOJ65462.1"
                     /translation="MELPSKYNPEGIESKWYQYWLKNGFFHSTPDQRQPYTIVIPPPN
                     VTGVLHMGHMLNNTIQDILVRRARMQGKNACWVPGTDHASIATEAKVVEKLAAEGIKK
                     TDLSREEFLNHAWEWTRKHGGIILEQLKKLGASCDWDRTAFTMDEIRSQSVIKVFVDL
                     YEKGKIYRGIRMINWDPKAKTALSDEEVIFKEQEGKLYYLKYKLCPTTENDNSDEYLI
                     VATTRPETIMGDTAVCIHPADPRYQHLKGRKCIVPLVNREIPIIEDEYVDMEFGTGCL
                     KITPAHDVNDYMLGEKHHLPSIDIFNDDGTLNENGGSYAGMDRFEVRKKIVKDLEVAH
                     LLEKTENYINKVGFSERTDVPIEPKLSMQWFLKMEELAKPALEAVMTDKIKLYPPKYK
                     NTYRHWMENVKDWCISRQLWWGHRIPAYYFSNGGWVVAETKEEAYQKALKIANEPFPI
                     ENLRQDEDCLDTWFSSWLWPISVFNGILEPDNEEINYYYPTDDLVTAPEILFFWVARM
                     IIAGYEYRGEKPFKNVYLTGIVRDKLGRKMSKSLGNSPDPLLLIEQYGADAVRMGMLL
                     TSPAGNDLLFDESLCEQGRNFNNKIWNAFRLVKGWQTAEIEQPEASRLAVEWFRHQLN
                     KSLYEMEDLFGKYRLSEALMTVYKLFWDEFASWYLEIVKPGYGQPVDLKTYNATLEFF
                     DVLLKILHPFMPFITEELWQQLSKRKDGESIMMQPHPQPENFDENIIKEFETVKEIVA
                     GIRSVRLQKNIPNKEVLKLLVNGKDNTNFYSVVKKLGNIKSIEKTAEKAPGSVSYMVG
                     TTEYIIPTGNKVDIASEIKKLEEELAYLEGFLASVMKKLNNEKFVANAQPQVVENERK
                     KKADAESKIASLRESIEALRKIS"
     gene            10934..13222
                     /locus_tag="PLZ54_00555"
     CDS             10934..13222
                     /locus_tag="PLZ54_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ65463.1"
                     /translation="MKKINLLFIVLSLVAVVNAQNKPATPQLGKDSLKEVIKAMTLED
                     KIYMVMGLGEDTWKNPATGPKTVLLAGAAGRTWDIPRLGITPAILTDGPAGLRIEPKQ
                     EGFSHPFYCTAFPTATVLASTWNTPLVEEVGKAMGNEVLEYGSDVLLAPALNIQRNPL
                     CGRNFEYYSEDPLIAGKMAAAMVVGLQSNGVGTSVKHFAANNIETKRTTINAIVSQRA
                     LREIYLRGFEIAVKEGNPWTVMSSYNRLNGFYTSQNPDLLTTILRKEWGFKGMVMSDW
                     WGGDDAVAQMKAGNDLLEPGATQREVLREALKNKTLSEKTLDRNLMRILDFIQKTPHF
                     KGYQPSYHPDLEAHAVIARKAAVEGMVLLKNENSTLPFKKVKKIALFGKTSYQFMTGG
                     TGSGEVNYDHAAALDEAFGTAKFQLSKTLTDRYRQFIDSVRQHEKKENQKFAVDFAPE
                     LNLTKDFIQSQVKNSDIAVITIGRQAGEGWDRPESTFSLSEVEKNLIKNVCTSFHAAK
                     KKVVVVLNIGGVIETADWRDWPDAVLLAWQPGQQGAHAVVDLLKGTENPSGKLAVSFP
                     MKYADVPSAATFPGEPKDNPVNAFYTEGIYVGYRYYETFKVPTAYPFGYGLSYTSFKY
                     SDLKLSNTLFDNEISVSVKITNIGKTAGKEVVQLYREAPKQAIDKPVKELKAFAKTQL
                     LKPNESEVITFKLDARSLASFWSGVSAWIADEGKYKIHVGASSEDIRATGEFSLPKKI
                     MVEKTHDVLYPNLLLHEISPEK"
     gene            13335..14015
                     /locus_tag="PLZ54_00560"
     CDS             13335..14015
                     /locus_tag="PLZ54_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HOJ65464.1"
                     /translation="MIQLEALYKISYGLYIVTSGDRNQGNGFVSNTVFQVTSQPQRFA
                     VCCNKDNYSASFIQQFKAFAVSILAQDTSAELIGKFGYKSGREMNKMTDVKVIFKETN
                     VPIVLDGSLAFFECKLVDVFDVGTHWIFIGELVSAEIVDDTGQPLTYAYYREVKKGFS
                     PKNAPTYIDQSKIETKTENAMSKKFKCPICGYIYDEAEEKVKFSDLPDDWVCPVCGSE
                     KSEFIEIS"
     gene            14051..14629
                     /locus_tag="PLZ54_00565"
     CDS             14051..14629
                     /locus_tag="PLZ54_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOJ65465.1"
                     /translation="MDKSLKGTRTEQNLLKAFAGESQARNRYEFFASVAKKEGYEQIA
                     AIFMETASQEKEHAKRFFKFLEGGMVEITASYPAGIIGTTKQNLKAAAEGEYEEWSSL
                     YPTFAEVAKEEGFPEISVAFKMVAKVEVEHERRYLKLLQNISEDKVFKKDGKVLWQCR
                     NCGYIYESEEALEVCPVCLHPKAFMQLKEDNY"
     gene            complement(14698..14868)
                     /locus_tag="PLZ54_00570"
     CDS             complement(14698..14868)
                     /locus_tag="PLZ54_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65466.1"
                     /translation="MKFQDMLRILLEKQRQWDSLTPEQQEAEKEKHVAYDECILSESG
                     YYDYELDEDEND"
     gene            complement(14886..15164)
                     /locus_tag="PLZ54_00575"
     CDS             complement(14886..15164)
                     /locus_tag="PLZ54_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65467.1"
                     /translation="MEWIIKQKEFERIFGEDMVQQNRFISSSKDVNVALDFAIRNQSL
                     MKKNEIQVVFEITSKNGRNISDISKLEIMFTSNTSFRIEKTETTDNDV"
     gene            complement(15133..15261)
                     /locus_tag="PLZ54_00580"
     CDS             complement(15133..15261)
                     /locus_tag="PLZ54_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65468.1"
                     /translation="MGKNTLTEFNQAAKTLIERGLEKLPVHNGMVNRGMDYKAKRI"
     gene            complement(15270..15452)
                     /locus_tag="PLZ54_00585"
     CDS             complement(15270..15452)
                     /locus_tag="PLZ54_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65469.1"
                     /translation="MHFDLSVRCSPDRKMLESYFRIKENYRDIAGVVRSYILLVPGSI
                     LELDPNQRKQVAIIGN"
     gene            16013..17584
                     /locus_tag="PLZ54_00590"
     CDS             16013..17584
                     /locus_tag="PLZ54_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOJ65470.1"
                     /translation="MHKEIQITLPPEKVNNKSVLEKEIIKIINIEIQQITRWRIIRKS
                     IDARSYLPKFNLSVEVYWDEIPPEDTAYNFHYNFVGNKPSVLIIGSGPAGLFAALKLI
                     ELGLKPVILERGKQINQRKKDIASICRNECIDPNSNYCFGEGGAGTFSDGKLYTRSKK
                     RGDKLHVLQVFHHHGANENILYEAQPHIGSDKLPLIVENICKTIIDCGGEIHFETRIT
                     DILLKNGHLTGCKTTDNNIFEAASLILATGHSARDVYEMLYHQGILLENKGFAMGVRV
                     EHPQQLIDSIQYHKKYRGDFLPPANYQLVEQVEGRGVYSFCMCPGGHIVPAGSGTKEI
                     VVNGMSASKRNSPFANSGIVVEIKPEDIPSDFSGCGPLASLMFQKHLEKLAYINNGGL
                     NLTAPAQRLTDFVNGKLSDNLPTCSYLPGLISSPLHFWMPDFLTKRLQEGFKKFDKKM
                     HGFLTREAVIVGVESRSSSPIRIPRDKETYQHPQIKGLFPCGEGSGYAGGITSSALDG
                     ENAAIAVYKSRLYHN"
     gene            18459..19070
                     /locus_tag="PLZ54_00595"
     CDS             18459..19070
                     /locus_tag="PLZ54_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ65471.1"
                     /translation="MKNTEEVILQKSLDLFIEFGIRNVSIDTICNELRISKKTFYSYF
                     KQKEDLVDAILTYEEKINIEEMKKRFDDKNAIESLISVLKEIKKNIDCQPKLMWMDIK
                     KYYPKVYEKHEMRRRSIIRKDFEKNLQQGVKEGFYRDDLDIELLSMLHSYQLKNIMTT
                     MSQYPKKYSKKRIVDFFIDIMIHIIANEKGLEYVRKNYYESEP"
     gene            19108..20460
                     /locus_tag="PLZ54_00600"
     CDS             19108..20460
                     /locus_tag="PLZ54_00600"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ65472.1"
                     /translation="MTANSLRKSIFIVLCVIALLPFSLKAQDTLIVSLDKAIEIALSE
                     SPTIKVANKEIQRVDYSSKEKISALFPVISASGQYSRAVKKQKLFFNIPGMPARPDGI
                     EVGQDNTFSGGISAAMPLIAPTLWATIKMSELDAQLVMESARSSKLSLVNEVSKAYYA
                     ILLAQDSYDVFKKSYDIAVENAKIIENKYKQGSVSEFEWIRADVQVKNANANLVSAEN
                     AVNLSVLRLKMLMGIDMFIGLKVEGNLSNYEANKYADVLSIDTTTLKSNSDLKQFDIK
                     AQQLKQSLKIQKSTNLPTLSAAINYQYMSMADDSIAFSNYHWFPTSTAALSLSIPLFQ
                     GGGKLFKQKQIEIQVEEMKYQRDNLRRGLELQAMSYLDNINQSLKVIESNKEGLRQAE
                     KALSISQKRYEVGAATYLDLSNAELAYTQAGLSYNQSIYNYLSAKADLEKLLGNQIIF
                     "
     gene            20518..21534
                     /locus_tag="PLZ54_00605"
     CDS             20518..21534
                     /locus_tag="PLZ54_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOJ65473.1"
                     /translation="MKRINKLGILIMALTIGLVSCGKSGKKQQGEEEIPSVRVQQVKM
                     QDVEQTGQFTATVQPEVKNNISPSTPGRIRKIMVEVGTSVSKGQKLVQMDAANLSNLE
                     IQVENYQRMYNRVAELFSVGGASQQELDNAKLQLDIAKTNLQNLSENTYLLSPTAGVV
                     TARNYDNGDFFNGQIPILTVMQINPVKLLINVSESNYAQVKIGMPATIKFDIFEGETF
                     QGKISLIYPTIDERTRSFQVEIKMNNENYKIRPGMFARVEINFGSARRIIVPDMAVIK
                     QSGSGEYFVYVYKDGSVIYQRVELGKRLGKEYEIISGLNDGDLVVVSSQSKLADGKKV
                     NLQK"
     gene            21602..24733
                     /locus_tag="PLZ54_00610"
     CDS             21602..24733
                     /locus_tag="PLZ54_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOJ65474.1"
                     /translation="MSLYENSVKRPIMTSLIFVGVIILGLYSLSRLPIDLLPDIEPNI
                     LMVATNYTGASALDIENNVTRPLENTLNTVENLKNIRSNSKENTSVIILEFEFGYDLD
                     VLANDVRDKLELVKPYLPEGASNPVIFKFSTDMIPIVMISATADKSLPALYKILDENV
                     ANPLARINGVGSVSINGAPEREIQVYVDPVKLEAYGLTVESIGQNIRLQNINTPSGSM
                     DIGNKTYTVRVQGEFKDPTELSKVVVGSYNGKNVYLSDVAIINDGHEERIQESYTNGV
                     QGATIIIQKQSGANTVEIANAIRAKLPQIEKNLPSDIKLNIINDTSENIKNTIAGLEE
                     TILYAFLFVILVVLFFLGRWRATIIVILTIPISLIAAFIYLSGTGGTLNIISLSSLSI
                     AIGMVVDDAIVVLENITKHIEKGSRPNSAAIHGTNEVATAVVASTLTIVAVFFPLTLV
                     GGLTGILFAQLGWMVTIIMVVSLVCALSLTPMLSSKMLRLNPHHSRMFTVVYSPIVKF
                     LDNVDAWYANIVNWSVRHRWQTVAIAAGFFVLSLIPLIGIGTEYIPAQDNSRISATIE
                     LPVGTRMEISKKTAENLTELWREKYPEIQTINYSVGQASSSNIIRSIQNTGSHIISFT
                     LRLSDTNERNRTIFEIADSMRVDLKSFLEINKANVTAGGAMGTMGGQSTLDIEIYGYD
                     FNETDKVAMELTNRINNIQGLVNTTISRGDYSPEYQVEFDRDKLALNGLNIGTASTFL
                     RNRINGLTASLYREQGDEYTIRVVYAPEYRQSIEDVENILIYNTMGQAIRLKDLGKVI
                     ETLTPPYIERKNRERVITVSSTISGTTLDKAVQAINTELKNMEIPTGVDTQISGSYED
                     QQESFADLTTLLLIVIILVYIVMASQFESLTYPFIIMLALPFAFSGVVLALFLTGGTL
                     NLMSMIGIIMLVGIVVKNGIVLVDYINLNRERGMGIVRAIVSGGRSRLRPVLMTTFTT
                     ILGMIPLAISSSQGSEIWKPMAITIIGGLTVSTILTLLIVPTLYAIFAGFGIKRTYNK
                     YKKGTNKLQQSEK"
     gene            24913..25209
                     /locus_tag="PLZ54_00615"
     CDS             24913..25209
                     /locus_tag="PLZ54_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65475.1"
                     /translation="MKAIFIVFDQGFYQQILESLYINHHVKGFTAWETVKGRGSKTGE
                     PHYGSHAWPSLNSAIITVVAEEKVKPILDDLKALDATSDVMGIRAFVWNIEDGM"
     gene            complement(25327..26022)
                     /locus_tag="PLZ54_00620"
     CDS             complement(25327..26022)
                     /locus_tag="PLZ54_00620"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOJ65476.1"
                     /translation="MNKIVVLTGAGMSAESGLSTFRDSGGLWEQYRIEEVATPEAFKK
                     NPEKVLNFYNERRKQLLKTVPNEGHEALAMLENFFDVYIITQNVDNLHEKAGSSRVIH
                     LHGELMKVCSTGPGHEVYELSSENIEIHVGDKCPKGYQLRPHVVWFGEPVPEIEKAIN
                     LVSQADIFIIIGTSMQVYPAAGLIRYVKPGTPIYLIDPKEVMVNTSNTTFIKKGASEG
                     IKELVQILKNTYL"
     gene            complement(26396..27076)
                     /locus_tag="PLZ54_00625"
     CDS             complement(26396..27076)
                     /locus_tag="PLZ54_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOJ65477.1"
                     /translation="MSIEKNLKKIRVQIPENVQLVCVSKFQPNKAILEAYHLGERIFG
                     ESRVQELCEKQKSLPGDIQWHFIGHLQTNKVKYIVPFVDLIHAVDSFRLLEEIDRNAR
                     KIGRVVRCLLEIHIARESSKFGFLPEQLIEMLKGEGWKNLHNVQICGLMGMATYTDNQ
                     TQIRQEFRNLKLLYDQLKDSFFSNEASFRELSIGMSNDFQIAIEEGSTIVRLGTCIFG
                     ERNCTVIT"
     gene            complement(27105..27572)
                     /locus_tag="PLZ54_00630"
     CDS             complement(27105..27572)
                     /locus_tag="PLZ54_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4494 domain-containing protein"
                     /protein_id="HOJ65478.1"
                     /translation="MHTWFECKIKYEKVIEEGKTLTVNETYLIDALSFTEAEKRIIQE
                     MEPYISGDFLVANIRRARINEIFAHDGGDRWYRCKVFFISLDEEKSVEKRTAVTMLVQ
                     ATSVKEAIEVLTSEMKNSLVDYELVSVSETPIMEVYPYVAEEKKVVQETSNFQ"
     gene            complement(27937..28809)
                     /gene="fabD"
                     /locus_tag="PLZ54_00635"
     CDS             complement(27937..28809)
                     /gene="fabD"
                     /locus_tag="PLZ54_00635"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOJ65479.1"
                     /translation="MKAFVFPGQGAQFVGMGKDLYEQSTLAKEYFEKANEILGFRITD
                     LMFEGTPEDLRQTKVTQPAVFLHSVISAFVLGEEFNPDMVAGHSLGEFSALVAARALT
                     FEDGLTLVSKRAVAMQKACELEESTMAAIIGLDDEIVEEVCDSIKDMIVVPANYNCPG
                     QIVISGTLQGVDMACEMLKERGAKRALKLSVGGAFHSPLMEPARLELSEAISQTTFSI
                     PICPVYQNVNAYPQTDPVNIKQNLIAQLTAPVRWTQTIKNMITDGADEFIELGPGDVL
                     KGLIKKINPNVIVH"
     gene            29175..29480
                     /locus_tag="PLZ54_00640"
     CDS             29175..29480
                     /locus_tag="PLZ54_00640"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOJ65480.1"
                     /translation="MKILRRIFLNKYTLVFLIFAGYVTFFDKHNLISRWKIYQKNKEL
                     EKEIKYYDEKIKSDRTKLFELKSSNENLEKFAREHYFYKKDNEDVFIINKKDSNKNR"
     gene            29571..30479
                     /locus_tag="PLZ54_00645"
     CDS             29571..30479
                     /locus_tag="PLZ54_00645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOJ65481.1"
                     /translation="MKKSNNFFKTIYQKYPYLFVLFLSLLLTIPWTMVSDFYTKGEPR
                     EASVAQCILNTGNWILPTDYADETAYKPPLMHWMIALFSLPAGKVSETTARLPSALGL
                     IGITVSFFVFLSKRTSKKRALLATLLMLTSFEMHRSGIEARVDMLLSFFMIASLICFY
                     IWEEKGLRKFPWFISLLLAGASLIKGPVGIVLPCLVFIVYLLLLQKYSFWKIIWKNFL
                     VALPAVFILLGWYYLAYRQGGEKFLNVVYAENFGRFLGQKSETLDIAYNLGHENPFWY
                     YIPAILTGFLPWSLVAIFSLFAVSYK"
     gene            30507..31340
                     /locus_tag="PLZ54_00650"
     CDS             30507..31340
                     /locus_tag="PLZ54_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65482.1"
                     /translation="MKESFKNLKNADKLVLFSTITLIVVVGFYSIPSSKRSVYIMPVY
                     PFAAYLLVLLYEWAMKRKPQFINWTANIIFILVGFLLLLMAFSRFVNLSAITSPLIKD
                     KTLHHDIGLYSKAFQHPGVAGVLFWLFLATAFVWYLKWKRTRILQNILFASIGLFIGL
                     QVFLEGFAYPIYKNGYSVRPFAEKLQNKYSLKNNVFVMNNLKEYPNLYALNFYLGNRF
                     KNFEKEKPEEGYFLTGEKSLKKIRKVYGTNYQFTVLENSGNRFNDIKDVILFCRLKRI
                     K"
     gene            complement(31735..31908)
                     /locus_tag="PLZ54_00655"
     CDS             complement(31735..31908)
                     /locus_tag="PLZ54_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65483.1"
                     /translation="MKDFLPYLGSIIILIGVALLAYYQFGGHPSNLILGTAGILMIVG
                     FFTHLFLNKKIVE"
     gene            32155..34110
                     /gene="dnaG"
                     /locus_tag="PLZ54_00660"
     CDS             32155..34110
                     /gene="dnaG"
                     /locus_tag="PLZ54_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOJ65484.1"
                     /translation="MIDQATIDRIMEAAQIQDVVSDYVTLKKRGVNMIGLCPFHNEKT
                     PSFTVSPAKGIFKCFGCGKGGNAVHFIMEHEQLSYYDALKFLARKYNIEVQERELTDE
                     EKELHNERESLLLVNEFAQKYFSDTLYHHIDGKTIGMSYFQERGIREDMIRKFQLGYC
                     LDQQDAFTQKALKAGYNKKYLVKTGLTIETENNRLTDRFRGRLIFPVHSLSGKIIAFG
                     GRVIKKDDKTAKYLNSPESEIYHKGNELYGMYFAKQAIVKEDRCLLVEGYTDVISLHQ
                     SGIENVVASSGTSLTPAQIRLIHRFTNNVTVLYDGDAAGIKASLRGIDLLLEEGLNIK
                     VVLLPEGEDPDSFSHKLNAVDLLNFIRDNSVDFIRFKTKLLMEDAANDPIKRSNLILD
                     IVRSIAIIPEPIVRAEYVKECSNLLQVNEEILYHEINRLKRKESEKKLGQSNVSASST
                     EPKPQPVSPQEMLNAENQRFEQEERNILQVLIKYGKSALYYSDEENRSPITVEDFIIE
                     DLEQDQLEFVHPLYAQMLQEYKEQCLKKNINVQNFFLHHIHPEISKLTAELISEKYTL
                     SKIYKKTRMDEPEIDKVREFAERAVLEYKNSIVLSQIKQKLLEMKIAAENHDDELVRI
                     LMIQISQLEEIKNQLAKNLGERIVIKL"
     gene            34123..34770
                     /locus_tag="PLZ54_00665"
     CDS             34123..34770
                     /locus_tag="PLZ54_00665"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HOJ65485.1"
                     /translation="MTYEELIHDTEYKELASLSHNEIKNFIVNEITENQQWARAANLY
                     QLTGILAFMLGTFKAFMPYFVHRQAYGLLWLLAGIVFTFTILIVFHELIHALAYWAMG
                     ARHLFFGCQPKKFMFYVQADKAVVGYRQFKIIALAPTVFISFLSLLGMIIFYHQPAFY
                     FFISIFGLHSLFAAGDFGLLCFFQNRPELEIVTFDVKEEGKTYFYGKEKRKNIAV"
     gene            34835..35605
                     /locus_tag="PLZ54_00670"
     CDS             34835..35605
                     /locus_tag="PLZ54_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ65486.1"
                     /translation="MILFDQIVFGPVHSRRLGTSLGINLLPVDAKICSFDCLYCECGF
                     NTSGKHAKLPTRMEVHNALEMRLKEMASDGQIPDIITFAGNGEPTLHPDFAGIIDDTL
                     ELRNKYCPNAKVGVLSNATQIHKPDVFNALKKVDDNILKFDSAFDHTLQLLDRPVRKY
                     ITVRWLIEHLKKFEGKMIVQTLFIRGEVNGEKIDNTSDKEIEAWLKALKEMNATQLMI
                     YTINRETPLKTLEKIPLEELNRIAEKAKSYGFDVSVSG"
     gene            35621..36565
                     /locus_tag="PLZ54_00675"
     CDS             35621..36565
                     /locus_tag="PLZ54_00675"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ65487.1"
                     /translation="MKILVCPLNWGLGHAARCVPIIRQLITEGNDVVIAADGFPLEFL
                     QLEFPELRFLKAPTQNVRYSTGNSQIWAMFKSIPRIFGNIFQDHCWLHRLLKQEHFDR
                     IISDNRFGCWSKRTYCIYITHQLMVKMPPELRLLEPIVWRLHRFFIHRYDECWIPDLA
                     GDENFSGDLAHRYPLPENAHFIGILSRFQQLKNIIPNYTYETVMVLSGPEPQRTIFEK
                     QLTEKFSKDTVKSLMIQGLPQEKQTSVQVGNLTKVSHLSTQEMASLLAGAKKIIARSG
                     YSTIMDLQALNCLDKAILIPTPGQTEQIYLAELHKKCR"
     gene            complement(36762..37160)
                     /locus_tag="PLZ54_00680"
     CDS             complement(36762..37160)
                     /locus_tag="PLZ54_00680"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOJ65488.1"
                     /translation="MEEFFLEKKSKFSFHKTLSELTNAIQEGGWRLVYVHDLQETMAR
                     NNKEVLPVKVLELCSPYHAYSILGKDEWRTFSSMMPCRISIYEKSDGNTYVSQMNISM
                     LAPLMEKEIQGIMNISYAEVQNFVSRIIEN"
     gene            complement(37176..37670)
                     /gene="trxA"
                     /locus_tag="PLZ54_00685"
     CDS             complement(37176..37670)
                     /gene="trxA"
                     /locus_tag="PLZ54_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOJ65489.1"
                     /translation="MKKLTGIFLMITFIALSACAGSNGENKQNNSQNKNEEKLMGTIH
                     LTKEEFLKKVVNYEASPNEWKYLGDKPCVIDFYADWCGPCKAVAPILEELAKEYDGQI
                     YIYKIDTDKEQELAAAFGIRSIPTLLFCPMNENPQMAQGALPKDVLKNAIDEVLLKKE
                     KVKQ"
     gene            complement(37983..38210)
                     /locus_tag="PLZ54_00690"
     CDS             complement(37983..38210)
                     /locus_tag="PLZ54_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6132 family protein"
                     /protein_id="HOJ65490.1"
                     /translation="MLKKFLSRHWLKVVGVLLGSIGGFAYFYFVGCKSGTCPISSNPY
                     ISIVYGAVLGYLFFDMFKVKSKNTNANDQED"
     gene            complement(38224..38529)
                     /locus_tag="PLZ54_00695"
     CDS             complement(38224..38529)
                     /locus_tag="PLZ54_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ65491.1"
                     /translation="MEQINNINIDQMEEAAYKLRAISHATRLCIISLLSEKEELNVSQ
                     ISEELNCEQSLLSHHLTDMRAKGILNCRRDGKNCYYSLKNKEIVQILDCIRSCQCTN"
     gene            38711..39763
                     /gene="aroB"
                     /locus_tag="PLZ54_00700"
     CDS             38711..39763
                     /gene="aroB"
                     /locus_tag="PLZ54_00700"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOJ65492.1"
                     /translation="MTSSSTIICENFIAELNEFIEYQKPDGIFVLVDENTKKFCLPLL
                     LQSEKLKNCQVITIPSGDENKNIHTAIEVWNYLSSHGATRRSLMINAGGGMVSDLGGF
                     AAATFKRGISYINVSTTLLGAVDAATGGKTGINFKGLKNEIGVFAPADKVLINVDFFR
                     TLDDKNLRSGFAEMIKHSLLDSREHWKNILKFNLDTIDYELLKHRVAESVKIKEQIVE
                     KDPKEQNLRKALNLGHTFGHAFESFSYSQHQPVLHGYAVMYGLVCELYLSHVKLNFPK
                     EDLLKLVTLAKENYGKISICCDDYEQLYELMTHDKKNVSDTINFTLLSEVGKIELNQT
                     ATKKEIFECLDFYQEM"
     gene            39823..40182
                     /locus_tag="PLZ54_00705"
     CDS             39823..40182
                     /locus_tag="PLZ54_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOJ65493.1"
                     /translation="MKKTLVIGASTNPERYAYKAAQRLLENGHEIELLGLRPGEIFGK
                     SIDTEKKQYPDIDTVTLYVGPKNQPEYYDYILSLHPKRVVFNPGTENPEFEALLEANG
                     IQAQEACTLVLLSTQQY"
     gene            40247..40963
                     /gene="dapB"
                     /locus_tag="PLZ54_00710"
     CDS             40247..40963
                     /gene="dapB"
                     /locus_tag="PLZ54_00710"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOJ65494.1"
                     /translation="MKLALIGYGKMGKAIERIAKERGHEIVCIIDIDNLNDFESDKFK
                     SADVAIEFTHPGVALSNYRKTFAAGIPLVSGTTGWTNELPALKKEVEEKGYTFFWSSN
                     FSLGVNIFMAVNKYLASLMNRFSDYDVEITEIHHIHKLDAPSGTAITLAEDMLENLER
                     KTCWVKEEAKQPHELAIKSIREDEVPGIHIIRYDASFDAIEFKHEAKTRDGFALGAVM
                     AAEFAVGKKGWLTMQDLLKF"
     gene            41064..42464
                     /locus_tag="PLZ54_00715"
     CDS             41064..42464
                     /locus_tag="PLZ54_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HOJ65495.1"
                     /translation="MTSEPHTSRFHQPRRQWIKAAIWSLIYILFIAWVGNFWWLLLLP
                     VIFDMFITKFIPWTWWKKTKNKTLRSIYSWIDAIVFALVAVYFINIYLFQNYQIPTSS
                     LEKTLLVGDFLFVSKASYGPRIPNTPLSFPLVQNTFPIINTKSYLEKPQWEYKRLKGF
                     DTIAHNDIVVFNFPTGDTVCLKQQNPDYYQLCYYYGRDVVRSRKDIFGEIVYRPVDRR
                     ENYVKRCIGLPGDWLQIKSNQVYINGEKQKPFPGVQFNYLVQTDGTRLSDELLTKLGI
                     SKDDRLILNTSQNPQEIMALGLDPSFPIYHFPLTGEMLQKLQRVTGVVKIVIEPEMYG
                     GDVFPLGGGKNWTRDNYGPIYIPKKGAVLELNAYNYPIYERVIRTYENNKLEYKNGIF
                     YINDRPEKTYRFKMDYYWMMGDNRHNSADSRYWGFVPEDHIVGRPVVVWLSLDKDKSL
                     FGGKIRWNRFFKDARR"
     gene            42556..43164
                     /locus_tag="PLZ54_00720"
     CDS             42556..43164
                     /locus_tag="PLZ54_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOJ65496.1"
                     /translation="MLSMSYLAPIDYFMTLTNARSAVIEQHEYYQKQTYRNRCRIAGA
                     NGVLDLIIPVEKTANNKATVRDIRISGHNDWQRQHWKAISSAYLSSPFFEYYLDDFRP
                     FYEKNWLFLWDFNMEIQNKIFELLEIYPKLEFTDKFIDKIEENILDLRLQFHPKKQPF
                     LKIPSYYQVFENKFGFISGLSIIDLLFNMGNESLLWLLGKAE"
     gene            43229..44245
                     /locus_tag="PLZ54_00725"
     CDS             43229..44245
                     /locus_tag="PLZ54_00725"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HOJ65497.1"
                     /translation="MELDWNNIGFGYVKTDYNIRCSYQNGEWGEFEVHEDEYLTLHMA
                     ATCLHYGQEVFEGLKAFRGIDGKIRVFRMDENAKRMQSSCKGIVMQPFPVDRFQEAVL
                     KVVKLNERFVPPYESGASLYIRPLMIGTSAQVGVKPSREYLFILFVTPVGPYFKGGFQ
                     TTPYVILREFDRAAPLGTGIYKVGGNYAAGLAADEKAHSLGYSAVFYLDAKEKKYIDE
                     CGAANFFGIKNNTYVTPKSTSVLPSITNKSLMTLARDMGMKVEQRPVPIEELSTFEEA
                     GACGTAAVISPIQRIDDLDENKSYVFSPDGKPGVICEKLYKKIRAIQYGEEPDIYGWT
                     TIVE"
     gene            44491..46599
                     /locus_tag="PLZ54_00730"
     CDS             44491..46599
                     /locus_tag="PLZ54_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOJ65498.1"
                     /translation="MSIFFLFTGAKADEGMWMLPYIEQLNIQKMNGMGCSLSAEDIYS
                     DKNISLKDAVIVFGNGCTGVVVSPQGLIFTNHHCGFDAIQQHSSVEHNYLKDGFNAVR
                     LEDEISTPGLSVKFLVKIEDVTNQVLSHLPDSLSEKSRNEKIYEISDSICKAAEKDTH
                     YLAEVKPFFAGNEFYLLVYEEFKDVRLAFAPPSSIGNFGGDTDNWMWPRHTGDFSVFR
                     VYATPDGKPAEYSKDNVPYSPKRFASVSNKGYQTNDFTMILGNPGSTSRYLTSFGVKF
                     RMETGNQARIDVRGIKQTIWRSFMRKDEAINIAYANKYASNSNYWKNSIGMNKAIQKL
                     NVLERKREQEAAFLEWTKSNPEREKKYKDVLKTLEDGYSTLYPYARALNYLRESLMSG
                     VELPRIAQKASQLISQNLPKDSLIKEIEEIYKDYRPEVDQAVFAAMLDVYKNSVDADA
                     LPAFYQEIQKKFKGNTTLYAASIFQNSAFSSVDKLKKNIQKKKIKIANDPALIFGNEV
                     RNTYEQISSGIYAQTSEKVKEAERIYFSGLREMYEEQGKKLYPDANFTMRMTYGKIGG
                     YQPADAVDYHYYTTTKGILEKENPTDPEFIVPDKLKKSILEKDFGKYLDPQTKDMHVN
                     FISNNDITGGNSGSPVFNGKGELIGLAFDGNWEAMSGDIVFEPELQRTISVDIRYVLY
                     VMDKIGGAQRLIDELSISGY"
     gene            46658..48058
                     /locus_tag="PLZ54_00735"
     CDS             46658..48058
                     /locus_tag="PLZ54_00735"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOJ65499.1"
                     /translation="MEKTSEEKKSFQWKDVFQKIISDLKTLTNLHEDTNIESTIASIK
                     KDVEFKGANVWILFFAVIICSVGLNVNSTAVIIGAMLISPLMGPINGVGLSIGIYDTE
                     LLRLSLRNLLIMILVGLVASTFYFLITPITDAQSELLARTRPTLFDVFIAFFGGCAGI
                     VAASRKEGKITVISGVAIATALMPPLCTAGYGIASGQLRFFFGAFYLLFINSFFIAIA
                     TFLVTHLLHFPVKQFVDIQRQKVVKRSIIIFSILTIIPSFFLAWNVVSETRFNSHTYR
                     YIQDIQQLDLFSEKEIINVSRKYSRKEKSLTLSVIGKPLTEEQIRYIQQLLPKYGLNN
                     VNLIIKQTGGTFDLGAQTAFIEKLLMDKDIQLQKKDSIIAALNNKLQLMQTSGVNELQ
                     LAREVKVQFPDIKQIAFSNSIFINLDHLTQDTVPTIFIQWEQTPPNKKALLSQMNEWL
                     KVRLNLEKLKIIEVEM"
     gene            48104..48817
                     /locus_tag="PLZ54_00740"
     CDS             48104..48817
                     /locus_tag="PLZ54_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOJ65500.1"
                     /translation="MKIDVCFSPALYPFYSSGNSKIAVIVDVFRASTTICKALMEGAS
                     AIIPVASLEKAEELKSRGYFVGAERNVEKCDFADAGNSPFDYYSEKVKGKEIVFTTTN
                     GTRAIELAAAENEVIIGAFCNISAVSDFCRNARKDVLVICAGWNNLFSLEDTLFAGLL
                     VHRLKCSGNFETSGDSAVAALVLWQQAEPDIKTFLHQSEHAKRLFTHHLEKDFDFCLA
                     QDTLPMVPFYDKSSQKILF"
     gene            complement(49574..50017)
                     /gene="rplI"
                     /locus_tag="PLZ54_00745"
     CDS             complement(49574..50017)
                     /gene="rplI"
                     /locus_tag="PLZ54_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOJ65501.1"
                     /translation="MEVILKEDVNNLGYKGDIVKVKDGYARNYLIPTKKAVIATDSAK
                     KMLEEEKKQRAHKLERIKNEALELAEKAKSINLVIEAKASSTGKIFGAVGPIQIADAY
                     EKAGLVIDRKVIVVDEPVKELGSYKATLKLHREVSVEISFEVIGV"
     gene            complement(50038..50310)
                     /gene="rpsR"
                     /locus_tag="PLZ54_00750"
     CDS             complement(50038..50310)
                     /gene="rpsR"
                     /locus_tag="PLZ54_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOJ65502.1"
                     /translation="MATNNNEEIRYLTPPTVDIKKKKYCRFKKSGIKYIDYKDPEFLK
                     KFVNEQGKILPRRITGTSLKYQRRVAQAIKRARHLALLPYVTDMLK"
     gene            complement(50314..50658)
                     /gene="rpsF"
                     /locus_tag="PLZ54_00755"
     CDS             complement(50314..50658)
                     /gene="rpsF"
                     /locus_tag="PLZ54_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOJ65503.1"
                     /translation="MNHYETVFILTPVLSDLQMKEAVEKFKTILQENGATITNEENWG
                     LRKLAYPIEKKSSGFYCLLQFDAEPSVVAVLETNYRRDERVIRYLTTRLDKYAYEYAE
                     KRKNVKSKEGKE"
     gene            50991..51593
                     /locus_tag="PLZ54_00760"
     CDS             50991..51593
                     /locus_tag="PLZ54_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOJ65504.1"
                     /translation="MSKVLYIKANAKPEGESRTFMIADKFVEAYKKHHPQDEIITLDL
                     YKEDIHFLTVEDVMIHMSNEVEETENAMKKYALQFKSADKYIFAEPMWNLGVPAILKA
                     YIDYVSIPNITFKYGENGPIGLCQGKKALNVITSGGDYSEGMAAEWDLCDKYLKTIMG
                     LFGVLDYTTIRASNLDVIGMDVDAIVNKAIKEAEELAKTF"
     gene            51716..51985
                     /locus_tag="PLZ54_00765"
     CDS             51716..51985
                     /locus_tag="PLZ54_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOJ65505.1"
                     /translation="MKSGIHPTNYRPVVFKDLSNGDTFITRSTINTKETIEIDGVTYP
                     LVKLEISSSSHPYFTGKAKLVDSAGRVDKFMNRYAKSLEKKKTQK"
     gene            complement(52043..52693)
                     /locus_tag="PLZ54_00770"
     CDS             complement(52043..52693)
                     /locus_tag="PLZ54_00770"
                     /inference="COORDINATES: protein motif:HMM:NF023074.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3256 family protein"
                     /protein_id="HOJ65506.1"
                     /translation="MKLINNFFTVIFFFLCAKISAQGIDKLYVGMPDALHPTLTSQQR
                     LELIEYAKAGQNDTTLNRYKKPVFLLKLDTLHQHLMVKNSGSTSFEMKVLDANSNEPM
                     IAIIRTVCAPICHSLIEFYDFKWNRLPVTFPYPQATDWLDLEKAQKTEEGVETFQKIL
                     KYTFVSLSFQEEGQIVMAFNNSIDFLSDEDKKLVFPCMNTAPLLFQWSDLKWIREK"
     gene            complement(52677..56987)
                     /locus_tag="PLZ54_00775"
     CDS             complement(52677..56987)
                     /locus_tag="PLZ54_00775"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOJ65507.1"
                     /translation="MQTKVEIGKIHYKLFNTIAIDDVYLEDQQQDTLLYVKSMDAHFN
                     LWKFFRGKYQFRSIDLDQLYGNIAIDKAGKTNFDFIVQAFKKPQGSKPANIEFQITRF
                     KLKNSSFRFVDARQNQGNSQTFNPKHLYLTNINSDIHLDIFHKDTIKASINHLSAHEA
                     SGLTLKDLRTKFSVSNKNIDIPYFDLWMPKSKIHFDSIRVDYATFSDLKNLNENVKLK
                     IPLRSSYFTFSDFAAFVPAFANLNTKMDAEIEIDGRISSLKFNQIRLKFGNSFLLNAK
                     ADFTGLPNMDETFIYANINDLHFEKNDLQDFVAGLTKKPFVLPKELNQLGMVRYNGNI
                     SGFLNNLVAYGKLRSNVGTIATDILLQYNTKSQYLAYNGTIKSDNIQLNKILSNNQFG
                     LFSFNFNTKGEKQANKNFQGTIEADIPEFQWNGYSYRDMKLKGNYDGSGFDGTFDVAD
                     ENIDAHFTGIVDMTKRLPVFDFSLNLAKANLNALKLTTKYSNANLSFNARTNMIGNSP
                     DNLNGFVLFDKIAFTNRNRTLNAGKIQFVSRTKNDETYFLISSDYVNGSISGNFIYSS
                     LDQTFKRLAYHYLPALSNKIPHENSHPNFINVDLKISDTGELTDILELPWQLKGNSYI
                     KGYYDEKNYKIDFTSNFPYFKFGKREFENIFLSLQNEEDKLSFITRAQMDGKNKPMHF
                     YINATASDNSVNTLLGWQNSDSITNAGEFKTLTKFAATDGKINAQISVLPSQIILMDS
                     VLNIFPSVVKYNSDGSLQVNNFKMEGSSQFLHVNGIASKSEKDSLQFTMNNLDLYFIM
                     NDLVKLKDPSLGGYFTGNATMYSAFQQPFFEAKLLVKDFSMNQSVLGDVNLYSYWDKE
                     KKEVVSNAHVLNNKDTVAYIDGVYVPGKDSIDFVYDMHHLNMGMLKRYFGDVVQNVRG
                     FATGKLRMFGPTKKMRFDGDLYVAEGQLKMALTQTTYFFNDTIRMRPTSVEFNNIKMY
                     DEEGNEGLLDGIMKHTGMFGDLKYDVMIRSNNILALNTHANANEIFYGKAYAGGTVHV
                     FGDVNVTNVWVDMVSRPGSKCYVTMPGSSTAADNSFVLFVNKNDTIKTQPDEQKGKKL
                     PGNTYNTKVNLQIGITPDAEIQFIIDPKAGDMLVGKGTGNLRFEFDTYSDIKLFGTYT
                     IEKGNYLFTLQNVIRKEFKIDEGSTLTWTGDLYNAQVKIKGIYSLMASLRDLMDESQL
                     SSLPRTTVPVNCILLLSGNLMKPNVEFDIDLPSSDESVKQMVKSIISTNEMMARQIIY
                     LLIFNKFYNPYLQSAANAPVGIGSSEAVSLVVSTLSAQMNNWLAQMINSNIISIGFDY
                     RQTDQLSSDVQAQFLIQPNNRLIINGNLGYRNDNVNLSNNTNKFIGDIDVEWLLTEGG
                     KFRLKAYNHTIDRYQLRSAKNTQGIGLIYKENFSNFDELFRYYWNILTFKKKNNEADQ
                     "
     gene            57270..58301
                     /gene="tsaD"
                     /locus_tag="PLZ54_00780"
     CDS             57270..58301
                     /gene="tsaD"
                     /locus_tag="PLZ54_00780"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOJ65508.1"
                     /translation="MNPIIILGIESSCDDTSAAVLRDGVMLSNVIANQSVHAKFGGVV
                     PELASRAHQQNIIPTVQEALRQADIDKKQLSAVAFTRGPGLLGSLLVGTSFAKGFAQA
                     LSIPIIDVNHLQAHVLAHFIRKDENDNSPQPQFPFLCLLVSGGNSQIVLVKAYNDMTV
                     IGQTIDDAAGEAFDKCAKIMGLPYPGGPHIDRLAKEGNPNAFRLNKPQIEGYSYSFSG
                     LKTSFLYLLRDKMKENSNFIEENLNDLCASLQATVVDILMNKLKKAAKDYNIHQIALA
                     GGVSANSALRQAFNDLATFDGYEVFIPPFAFTTDNAAMIAMTGYFKYLDHDFSSFDDV
                     PFARVTNTF"
     gene            58309..58902
                     /locus_tag="PLZ54_00785"
     CDS             58309..58902
                     /locus_tag="PLZ54_00785"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HOJ65509.1"
                     /translation="MKKELSRIKKNLTPYNGVIYFVVILLICHFVWKLLVRGDDSNRQ
                     VTFLGNDISAPFQFMANHVAQTTASVLNFLGFHIQLFANNVLKQENGFGIQIVWSCTG
                     LKQAYIFFCIIAFYCGPMRKKLWFIPLGLAVVYVFNIFRIAAITALVQQHSSWFTFLH
                     EYFFKYLFYAVIFALWVLWEEKFVTKKEKNETHMTQT"
BASE COUNT        18454 a        10801 c        11044 g        18658 t
ORIGIN      
        1 tcctaccatc caaaacctta ttcgcttatt gaaaatctgg agattttatt tttaactcga
       61 agtagctttg aggctacgcc gaacggctgg taaaccgcat aatagcaagg tacctttttg
      121 tgaaaattga cagttattat ttaaattcaa tcataaattc ggtgagactt tttgttgaag
      181 ggagtccaat ttttttgcgg atgcgggctc ttcccatttc tacgcctttg agcgaaatat
      241 tcattaaatg ggcaatgtcc ttgctcgata gattgaggcg aaggtaagca caaaatctta
      301 aatcgttggg tgtgagttcg ggatatcgtt cgtgtagatg tcggaaaaag ttttcgtgaa
      361 ttcggtcgaa attggcctga aaaattgccc aatcatcttc tgaagaaaga ttttcgtcga
      421 gcagccgaat gattttttca taatacttgt tggggtattg tgatcctaaa gctgcttttt
      481 gttgcatgat ttcttctttt atggtggcca acacttcatt ttttctgatt agcgtcatgg
      541 tcgaagtggc caattccttg ctcttcagcg tcaattccga ttccagcttt tctttttcta
      601 atgtcatgat ttgctgttct cttttttcta tttcttttcg cctgatgtct tcctgttcga
      661 ttctgacctt ttcctttttc ctgtgaataa ttcgttgaac ataccagtag attccccaaa
      721 gtaaaaatag caccgccaaa gtgtagataa tttttgcagg cgtactcaaa taaaagggtg
      781 gttgcacttc aaatgcaaaa tttgtttctg agagaatttg tccactttcg gatattgctt
      841 cggctttgaa tacatatttc cgatagggca aatatttata ttcgatactg ggcgatgaag
      901 atggatctct ccaaaccacg tccaatcctt cgagtttaaa acgatacatc acattgtttc
      961 tttgagcata ttcggggtaa aagaccgaaa aaacaacatt gtttctggaa taaggaatag
     1021 tcggtaaact ggtggcttcg agcggtaaaa agttcaattt cttgttggct gcatccgaaa
     1081 tttgaatgct tttcagtttt aactgtatgc tgtactgtgg aatgagtttt tgtgtgtagt
     1141 tataaagtgc cagtccattc tctaaagtga aaatacattc attttctgag ataggaatga
     1201 tattttgata atcgtcaacg gtttgatgaa gaaaatcggc atattggacc atatccacca
     1261 atttgatttg accgggtttg atttcaacca gtgcagcttc gcccgggcga atgaaccaat
     1321 acagatttcc tttgaaatag catatccgat aggaattgga aaaatttccg agcgctttat
     1381 ttaattcgtc gaaaggcaca atttttttac gtatatcgtc gaaagtataa aataaattat
     1441 agtctgtaaa tactactctg ttgtttacag aaaagacgtt aatggtgttt aaatgttgct
     1501 gatcaagaga tttgtatatg tttatgtgtt caattttccg taagtcgggc gtaagttgaa
     1561 tggcaaaaag cccgcgatgc aaatgacttg cccaaatggt tccggtgtaa tctatttcga
     1621 tgtagcgaat gggatgaaga aaatcttcca ctgcatggct gaatttccat ttcccattct
     1681 cttttttgta aatacacagt tgcgtatagg ttccctgaac caatacctcc acaccatgaa
     1741 tgtttccatg agcgaaacac atgcctcctt ttacgggtga aacaggaatg gaccttgtgg
     1801 gtgtaacttc aaaggtttct tcattgttgc cacaaaattg ctgaccatca aaggaactta
     1861 catcccaaac ttgtcctttt atttttgggt cgatggaaag gttacgaatg gagtgatgtt
     1921 ccaacaaggc tctgtataat ccctgattgg ttgcaatata aatatgtgga ggatcataac
     1981 ttaccgaata aatggcgcca attgccgggt aaaaggattg aatgcagcga aaggatgagt
     2041 tttgatgaat aaaagcaatt cctttatcca acgccagcca aatattgttg tcgcggtcga
     2101 aagtaattcc cagaacggta ttgttttgaa ggacatttga agaactgatt gcccagatct
     2161 ttcgtcccga cttgtctata gccacagctc catttaaaat ggtaccgatc acaatcaatc
     2221 cgtttggcga aataacggct ctgttgattt gtgctttttt cagtacatcg tccacttcag
     2281 tggcaaaacg ggtaaactta tctctatcat aaagataaag accatcggaa atcgtaacca
     2341 ttaaagcatg tttatcgtca aaaggcagaa ttttgattac cggacttttc atgatattgt
     2401 tgggaactgg aacaaaagta ttttgttgcc ggtcgaagta gcaaaaatct ttctgaaaag
     2461 tatgtgtgta taaagtattt tgatagaggt tgaagaacaa gaaggagaaa tcggttctga
     2521 atccttttac ttctccgttt ttaaatgtaa aatatgaagt aaaggattga aaaaaaatgg
     2581 tgccctgatc gtcaaagata ttccaaactt catcgttctg cattttgtaa ttttttagca
     2641 aggcagacag ggaagtataa tttaattgtc caaaacggtc tctctcaaaa tagccaaatt
     2701 cttcaaaagc gccaatgtaa attctgtttt ttttgtcgat cataacagaa cgcaccagac
     2761 catttccggg aattttgtgc aatttccaca gagacccgtc aaattcgagc agtccttcgt
     2821 tgttcccaaa atacaggatg ccgtccaaac cttgtgctac cgcccaattt tgattggcag
     2881 cgcgataatc gttttttgaa aattgagtaa cgagcggaat aaattcctga gcatttgctg
     2941 cgcaagcaca gaaaataatg aaaagcagat ggattttttt cataaaggtg tacatgtcaa
     3001 tttttcaagg tattaaccgg agttctggtc aaaaagttca taagattata aaatgatttt
     3061 ttcgacaaaa tcaatccgta aattcttgca ggaacatcag aaattaagaa tctcaaaaat
     3121 aagaaaatat ttgttgactc aggagttaat aaggatattt tttagaaata accggcttaa
     3181 taaaaaaata aactgaattt ggcaatctca ttcttaaatt cagattttaa tttttaaaaa
     3241 tactttttat caacgcaact atttaattac caattatata gaaacatttt taatcatttt
     3301 aaaataagtc aaagctattt caaaatacaa acgcccaaag ttttttaacc ctgagcgttt
     3361 gctaagaatg taatgataaa ctcaaactac tttgtaaata tcgatgtttt ccatgtcgaa
     3421 agttttgatg aaagtagcat gttttctggt ttcaacttca ccataattga tatatacttc
     3481 agccgatttg cggaacatat cgtttcgggt agcgtcgaga atcagcaaat aacccatgat
     3541 gatgtgccct gccatttcta ccagtcgtcg agcctgaaaa tcgatatatt cctgattttt
     3601 agtttcgata acacggctca ccgaattttc atacaggttg gtcataatca tcaatctttt
     3661 tctgaaagaa tgaaattcgg gattgatggg taatttttca tattcacgta tttgctgaag
     3721 ataagttccg gtagtaacgt ggcggatggc agctacaacc tgtaattggg aagttccttc
     3781 atatatggtc agaatacgtg catcgcgata aagtcgttcg caggcatagt ctttcataaa
     3841 tcccgaaccg ccatgaatct gcacacagtc gaaagcattc tggttagcat attcgctggc
     3901 aaacattttc aaaagtggag taaaagcatc ggccagacgc tgaaattcct tgtattcggc
     3961 tttttcttca ggttcgagac ggcgctcttt ttcaatggct tcgtacgctt tgtacatatc
     4021 gacaaaacgg gtagtttcgt acaacaaggc acgtgaagca tcgagcttag cctttataag
     4081 tgaaatcatc tcaaaaacag caggaaattc aataatggct tttccaaact gctgacgttc
     4141 tttggcatac ttcaaagctt cattatatgc tgaagccgaa agtcccaccg attgggctcc
     4201 tacgccaagt cgggcactgt tcatgagcga cataacgtat tttatcaatc ccaagcgtct
     4261 ggaacctatg agttttgcag gcgtattgct aaaaaccagt tcgcaggttg gagagccctt
     4321 gatgcccagt ttgttttcga tacggcgaac ttttactgta tcatcgtgtt tgttgtccac
     4381 aaggaaaagc gacaatccac gaccatcggt agtcccttct tcggaacggg ccagcaccag
     4441 tttaatgtga gcatcgccat tggtaataaa acgcttaact ccattaagat accaaattcc
     4501 gtccttttcg ttaaaagtag ctttcagttg cacagcctgc aaatcggaac cagcatcagg
     4561 ttcagtcaaa tccattgaac aggtttgtcc ctgacatact ctcggaagaa attcccgttt
     4621 taagtcttcg tctgcaaatt cgttgatagt ttcagcgcag tcctgcaaac cccaaatatt
     4681 ggcaaaaccc gcatcggcac gcgaaaccat ttcagcagcc attacgtacg gcaccatcga
     4741 aaaatttaat cctccatact gacgtggcaa tccaattccg taaaggctgg cttgcgtcag
     4801 aacttcatgg ttttgttgtg tcccgggagc ataaacaaca cggttgttga caacttgagg
     4861 ccctgtatga tctacttctt cggcattggg agctatcact tctccacaaa tttcgccgat
     4921 aatttccaaa attttttcat aattatcaat ggcatcttca aaatcgcgtg aagcataatc
     4981 atatttgtcc ttgtcggcaa aatttctttc cttaagtgta acaattttct gcatcagggg
     5041 atgcgaaagt tggaatttta atgcgggatt atcgttataa aagttcataa ttttacggtt
     5101 attaaattaa gaaaacaatt gaagcgcgaa taataacagg aaacgtttaa ataaatttta
     5161 ttgattcgat tttctgaaat tttttcattc gctgggtttg tttcttattt tgtgtttttc
     5221 ttataatatt ttatcatttt cggaagcaca tcttccactc ttccggagat tacgtaatcg
     5281 gcaattttat tgataggtgc attgggatca ctgttgatgg caataatcat ggcactttcc
     5341 tgcattccgg caatgtgctg aatctgtcca gaaatgccac aggcaatata cagttttgga
     5401 cgtacggtcg ttccggtttg tcctatttga cgttcgtgtt cggcaaaacc tgcatcaaca
     5461 gcagcacgag aagccccaac ttctcctccc aaaacttctg ccagctcata aagcaatttg
     5521 aaattttcgg cacttcctac tccatatcct ccggaaacaa taattggagc cgacttaata
     5581 tcaattttag ttttttcgat atgtcgttca atcacctcaa ttacaaagtc ttggtccgaa
     5641 acgtatttcg aaacttccaa tcgacgaatt tcgcccgtat gttttggatc gaaaatctcc
     5701 tttttcatta cgccttcgcg tacggttgcc atttgtggcc tgcaatcggg gttcacaata
     5761 gtagcaacta tattgccacc aaaagccgga cgaatctgat aaagtaaatt cttatacact
     5821 ttaccggctt ttttgtcttc atagtcgcca atttcgagag aagtacagtc ggcggtcaat
     5881 ccgctgaaaa gagcagaaga tactcttgga cccaaatcgc gcccaagtga agtagcacca
     5941 agaagtgcaa tttgtggttt ttcttcctta aaaagatgaa ttagcaccga agcatgaggt
     6001 tgtgtggtgt aaggaacaaa tcttttatcg tcagccaaat gcaatacctc tactccataa
     6061 ggaaatacct gtttaggtat attttccagc tgataaccaa ttgctacagc ttccagcttg
     6121 caattgagct gttttgccaa cgaacgtccc tttgtaagca attcgaggct tacatctttc
     6181 actattccgt cttctatttc aagatataca aataagttgt tcatatatat ttaattcttt
     6241 tttcctgaaa ggaaattttc tatttttttt gccaataaat tagattagaa aaatttttta
     6301 gagatactca aaattaaccg atagtatggt tagcaatgag ttctttcatt aaactttcaa
     6361 tttcaaagtc gctgcctgtg agtcgtttac tttcttttgc cgtgaaaacg atattgtcga
     6421 ttttcttaac tttagtaggt gaaccgctca atccaagcca ctttggatcg tgttcaatgt
     6481 cattggcacc ccattcggta atgttcaaat agggatgtgc atcgtacaga tcggtataat
     6541 cagaacccga ttcctgtttt tcggatgggg ttgcagcatg tttgtattta agtgttaact
     6601 tggcgtttct tgggcggcat ggagctgctg ttccgttaac ggtaaccaat atgggcagtg
     6661 gccctttgac aatttccaca cctctttcca gtcggcgttt aacggtgata tttttcttgg
     6721 taatttcaat aatgtcttcg gcgtatgtga tttgtggtaa gcccagtttt tccgcaacct
     6781 gaggccccac ttgcgccgta tctccatcta tggcttgacg gccgcaaagg atcagatcga
     6841 tttttcccaa cttgcgaatg gcgcaggata aagcatagga agttgccaac gtatcagagc
     6901 cggcaaattc tcttccactt aaaagaaatc cgtcgtcagc cccgcgatag agactttcac
     6961 gaatgatttc ggcagcacga accggaccca ttgtcagcaa atgaacaact gtgccgggaa
     7021 ccttttcttt gatgcgaagt gcctgctcaa gagcattcaa atcttccgga ttgaaaatag
     7081 ccggcagcgc agcacgattc actgttccat cggctttcat ggcatctttt cccacatttc
     7141 gggtatcagg cacctgtttg gctaaaacta cgatattgaa actcatttta tcattaaatt
     7201 attgtaatta atattagtct ttcttcttca actgcgaaaa gttttttgtt acagcaaagc
     7261 tgaagaattt tatatccgat tttttttgat ttgcaaaatt agtcgttttt attgaagttg
     7321 caatttgttg tctctaatat aaaatttgag gcctttattc aataaaaatc gcgagacatt
     7381 tcacaaggcc tcgcgttttt taataggtat tagtttttaa ggtataaaaa agattgtgtt
     7441 gtttagttaa cgcttgcaaa atacgcatta aaaaataaaa cctgcaagtc aaaatttatg
     7501 ttttacaacc tatttttgga gcaattcgac ttttaacaat tttcagataa ttatatttta
     7561 tgaacttaat ctattaattt aaagacactt aaattgattg tggcttgaaa tgttaaaaat
     7621 attactgttc tatcgaatca taaacaagta ataagttttc taaaagtcga acttcttttt
     7681 tatacctctt gaaccaataa ttttaaaaaa cgtcgaagcg catcttcgat gaaaataaga
     7741 aatatctcaa aatttataaa atcgatgtgg aatccatctc aaaatttgct aataaatttt
     7801 ttaatagctg aaattgaatg gtgacaaagt ctgatcaaga aatttttcgt aaagcctcaa
     7861 ttgattccct caaagaagca attttgcttt cagcatcggc ttttttcttg cgctcattct
     7921 caacaacttg aggttgagca ttggcaacaa acttttcgtt gttcagtttt ttcatcaccg
     7981 aagccagaaa gccttcgaga taagccaatt cttcttccaa cttctttatt tcggaagcta
     8041 tatctacttt gttgcctgtc gggataatgt attcggtcgt tccaaccatg taagaaacag
     8101 agccgggtgc tttttccgct gttttttcta tcgatttgat attcccgagc ttttttacga
     8161 ccgaataaaa atttgtattg tcttttccgt taactagtaa ctttaaaact tctttgttgg
     8221 gaatattttt ttgtaaacgt acactgcgaa ttccggccac aatttcctta acggtttcaa
     8281 attcctttat tatattctca tcaaagtttt caggttgagg atggggctgc atcataatac
     8341 tttcgccatc ctttcttttg gacagctgtt gccacaactc ttccgtgata aaaggcatga
     8401 agggatgcaa tatttttaat aaaacatcaa aaaattccaa cgtagcattg taagttttga
     8461 ggtcaacagg ttgcccgtat cccggtttta caatttccaa ataccatgaa gcaaattcgt
     8521 cccagaaaag cttgtaaacc gtcattaatg cttccgacaa acgatatttg ccgaacaaat
     8581 cttccatttc atacaacgat ttgttgagtt gatgccggaa ccattccact gcaagccgtg
     8641 aggcttcagg ttgttcaatt tcggcggttt gccagccttt taccagtctg aatgcattcc
     8701 aaattttgtt gttgaaattt cttccttgtt cgcacaaact ttcgtcaaaa agtaaatcgt
     8761 ttccggccgg ggaagtcaac aacattccca tacggacggc atcagctcca tattgttcta
     8821 tcaaaagcag cggatcgggt gaattcccca gcgatttgga cattttcctt cccaatttgt
     8881 ctcttacaat accggtcaga taaacgtttt taaaaggttt ttcgcctctg tattcatatc
     8941 cggctatgat catgcgtgca acccagaaaa ataaaatttc cggagccgta accaaatcat
     9001 cagtgggata ataatagtta atttcttcat tgtcgggttc gagaatacca ttgaaaaccg
     9061 atattggcca caaccacgaa gaaaaccatg tatccaggca gtcttcgtcc tgtcttaaat
     9121 tctcaatcgg aaatggttcg ttcgctattt tgagtgcttt ttgataagct tcttcttttg
     9181 tttcagcaac tacccaacca ccattcgaaa agtaataggc agggatgcga tgcccccacc
     9241 aaagttgtcg ggaaatacac cagtctttta cgttttccat ccaatggcga taagtatttt
     9301 tgtattttgg aggatacaat tttattttat cagtcattac ggcttccagt gcaggtttgg
     9361 caagttcttc cattttcagg aaccattgca tggataattt tggttctatg ggaacatccg
     9421 tacgttcaga aaaaccaact ttattgatgt agttttccgt tttttcgagt aaatgggcta
     9481 cttcaaggtc ttttacaatt tttttgcgga cttcaaaacg atccatgccg gcgtagctac
     9541 caccattttc attgagtgtg ccatcatcat tgaaaatatc aatggaaggc aagtgatgtt
     9601 tttcgcccag catgtagtcg tttacgtcgt gagcgggtgt aattttgaga catccagttc
     9661 caaattccat gtcaacatat tcgtcttcaa taattggaat ttcccggttt accaatggaa
     9721 caatacattt tctacctttc agatgttggt agcggggatc ggcaggatgg atgcaaactg
     9781 ccgtatctcc catgatggtt tcagggcgtg tggttgcaac aatcagatat tcatcactgt
     9841 tgtcgttttc ggtagtcgga caaagtttat atttcaagta ataaagtttt ccttcctgtt
     9901 ctttaaaaat tacttcttcg tccgacaaag ccgtttttgc tttcgggtcc caatttatca
     9961 ttctaatgcc gcgataaatc tttccctttt cgtataaatc cacaaaaact ttgattacac
    10021 tttgtgaacg gatttcgtcc attgtaaaag ctgtacgatc ccaatcgcat gaagctccca
    10081 gtttttttag ctgttccaga ataatgccgc catgtttccg cgtccattcc caagcatgat
    10141 tcaaaaactc ttcacgtgaa aggtcggttt ttttaattcc ttcggctgca agcttttcca
    10201 caactttagc ttcggtggct atggaagcat gatcggttcc cggcacccaa caggcatttt
    10261 ttccctgcat gcgagcccga cgtaccaaaa tatcctgaat ggtattgttg agcatgtgtc
    10321 ccatgtgaag tactcctgtt acattgggag gcgggattac aattgtgtaa ggttgacgtt
    10381 gatcgggtgt tgaatgaaaa aaaccatttt tcaaccaata ttgataccat tttgattcaa
    10441 ttccttcggg attgtatttg ctcggtagtt ccatttttta tacttttaca atttcaaagt
    10501 gatgctgctc gatgagttgt gaaaatttaa ttatttgtct atcaaattga tagtgaaagt
    10561 ctttgtctta tttttttgaa atgcaaagat aatcaaaatt tgcaccaact gacgaatttt
    10621 tagaccgaaa aaatttgaat cgagaagctg aacctttaag gcgaacaaag gcctgttcta
    10681 atttatttgt catgagcaat tttaatgtca gttgtatttt gaaatgataa cggtagatat
    10741 agaacttggt agaaagacgt aaaaagtacg gtaaaatttt tatcgaacag ttattgtgta
    10801 cagtttttta acctttcaac aggaacgatt tgatatattc ggtatttttt cagaaaatcc
    10861 attcctttct catttaaaag gataataaaa tagggatatg cttttttctc aataacaaaa
    10921 aaaacataat ggtatgaaaa aaatcaattt gctttttatc gtgttgtctt tggttgcagt
    10981 tgtaaatgct caaaataagc ctgcaacgcc gcaattggga aaagacagct taaaagaagt
    11041 catcaaagct atgactttgg aagacaaaat ttatatggtc atgggtttgg gagaagatac
    11101 atggaaaaat cccgctacag ggcctaaaac cgtattgctt gccggtgctg ccggtcgtac
    11161 atgggacatt cctcgattgg gcattacacc tgctattctc actgatggcc ctgccggttt
    11221 acgaattgaa ccaaagcaag aaggcttttc tcatcctttt tattgtacgg cattcccaac
    11281 ggcaacagtg ctggcttcta cttggaatac tccactggta gaagaagtag gaaaagctat
    11341 gggtaacgag gttttggagt atggttcgga tgtattattg gctccggcat tgaacattca
    11401 gcgaaatccg ctttgtgggc gaaattttga gtattattcc gaagatccgc tcattgccgg
    11461 taaaatggcc gctgcaatgg ttgttggatt gcaatccaat ggcgtcggca cttccgttaa
    11521 gcattttgct gccaacaata tagaaaccaa acgcaccaca attaatgcaa ttgtcagtca
    11581 gcgtgcactg cgagaaattt atctgagagg ttttgaaatt gctgttaaag aaggaaatcc
    11641 ttggacggta atgtcttcct acaaccgctt gaatggattt tatacttctc aaaatcccga
    11701 tttgctgact accattttac gtaaagaatg gggattcaaa ggaatggtaa tgtccgattg
    11761 gtggggtggc gatgacgcgg tagctcaaat gaaagcagga aacgacttgt tggaacctgg
    11821 agctacacag cgagaagtac tcagggaagc actgaaaaac aaaactttaa gcgaaaaaac
    11881 gcttgacaga aatttgatgc gaattctgga tttcattcag aaaactcctc atttcaaagg
    11941 ttatcaaccg tcgtatcatc ccgatttgga agctcatgcc gttattgctc gcaaggcagc
    12001 agttgaaggt atggttttat tgaaaaacga aaattccact ttgccattca aaaaagtaaa
    12061 aaaaatagct ttgtttggaa aaacttccta tcaatttatg actggcggaa ccggaagtgg
    12121 cgaagtgaat tatgatcatg ctgcagcttt ggatgaggct tttggtactg caaaatttca
    12181 attgtcgaaa actttgaccg atcggtacag acaatttatc gattcggtaa gacaacatga
    12241 gaagaaagaa aatcaaaaat ttgctgttga ttttgcaccg gaattaaatt taaccaaaga
    12301 ctttattcaa tcgcaggtaa aaaattccga tattgcggtt attactattg gacgtcaggc
    12361 cggtgaaggt tgggatcgtc ctgaatcgac tttttcattg agcgaagttg agaaaaatct
    12421 gataaaaaat gtctgtacct ctttccatgc tgccaaaaag aaagttgttg tggtgctcaa
    12481 tattggaggc gttatcgaaa ctgccgattg gagagattgg cctgatgctg ttttgcttgc
    12541 atggcaaccc gggcaacaag gtgctcatgc ggtggttgat ttgttgaaag gtacagaaaa
    12601 tccttccggc aaactggctg tcagctttcc gatgaaatat gctgatgttc cttcggctgc
    12661 aacttttccc ggcgaaccca aagataaccc cgtcaatgct ttttatacag aaggtatata
    12721 tgtcggttat cggtattatg aaactttcaa agtgccgaca gcttatcctt ttggttatgg
    12781 actttcctat accagtttca aatattccga tttgaagttg agcaatactt tgtttgataa
    12841 cgaaataagc gtttcggtaa aaattaccaa tatcggaaaa acagccggca aagaagttgt
    12901 tcaactttac cgggaagctc ccaaacaagc catcgacaaa cctgtaaagg aactgaaagc
    12961 ttttgccaaa actcaattgc taaaacccaa cgagagtgaa gtgataactt ttaagctgga
    13021 tgcccgttct ttggcttctt tctggagtgg tgtcagtgct tggattgctg acgaaggcaa
    13081 atacaaaata catgtaggag cctcatcgga agatattcgc gctaccggtg aattttcttt
    13141 gccaaaaaaa ataatggtgg aaaaaacaca tgatgttctt tatcctaatt tattactgca
    13201 tgaaatatct ccggaaaaat gagatgagaa aatttatttt tgtttcaata tcgttataat
    13261 ttgaaattta aaactatttt tgtctaagat tttttttaga tgttttttca aatatctttt
    13321 ttaattctta ctctgtgata cagttagaag cactttataa aatatcttat gggttatata
    13381 ttgtaacttc tggagataga aaccagggaa atggttttgt ttcaaatacg gtatttcaag
    13441 ttacttctca gcctcagcgt tttgccgttt gctgtaacaa ggataattat tccgcttcgt
    13501 ttattcagca gttcaaggct tttgccgttt ccatactggc acaggatact tccgccgaac
    13561 ttattggcaa gtttggatat aaaagcggaa gggagatgaa taaaatgacg gacgtaaaag
    13621 ttatttttaa ggaaaccaat gtccctattg tactcgatgg cagccttgct ttttttgaat
    13681 gtaagctggt tgatgttttt gatgtgggaa cacattggat ttttattggc gaactggtta
    13741 gtgccgaaat tgtcgacgat accggtcaac ctttgactta tgcgtattat cgggaggtaa
    13801 aaaaaggatt ttcacccaaa aatgctccaa cgtatataga tcaatcaaaa atagaaacta
    13861 aaactgaaaa tgctatgtcg aaaaaattca aatgtccaat ttgcggctac atttacgatg
    13921 aggccgaaga aaaagtaaaa ttttccgatc tccccgatga ttgggtttgc ccggtttgtg
    13981 gttcagaaaa atcggagttt atagagattt cttaaataaa tcattcagta actcattaaa
    14041 aaacaaaatt atggataaat cgttaaaagg gacaagaaca gaacaaaact tgctgaaagc
    14101 ctttgccggc gaatcgcaag cacgtaaccg gtacgaattt tttgccagcg ttgccaagaa
    14161 ggaaggttac gaacaaattg ctgccatctt tatggaaact gccagtcagg aaaaggaaca
    14221 cgccaaaaga ttcttcaaat ttctggaagg tggcatggta gaaatcaccg cttcctatcc
    14281 tgcaggaatt atcggaacaa ccaaacaaaa tctgaaagct gcagctgaag gagagtatga
    14341 agaatggtca agtttatatc caacatttgc tgaggttgcc aaagaagaag gatttcctga
    14401 aatttccgta gcattcaaaa tggtggctaa agtagaagtg gaacacgaac gtcgttatct
    14461 gaaactttta cagaatattt ccgaagacaa ggttttcaaa aaagacggca aagtgctgtg
    14521 gcaatgcaga aattgtggct acatttacga atcggaagaa gcgctggaag tttgtccggt
    14581 ttgcttgcac cccaaagcct tcatgcaact gaaagaagac aattattgaa aaactttctc
    14641 gaaaaaattg aactctaatt gagcaggtag ggggattact cccccgccct actcgattta
    14701 gtcgttttca tcttcatcaa gttcataatc atagtaaccc gattcggaaa gtatgcattc
    14761 atcatacgct acatgtttct ctttttcggc ttcttgttgt tccggcgtaa gcgaatccca
    14821 ttgacgttgc ttttccaaaa gtattcttag catatcttga aatttcataa ttctgtcaat
    14881 ttacctcaaa catcattatc ggttgtttcc gttttttcaa tgcgaaacga tgtgttactg
    14941 gtaaacatta tttctaattt tgaaatgtca gatatgttgc gaccgttttt actcgttatc
    15001 tcaaatacta cttgtatttc attctttttc ataaggcttt gatttcttat ggcaaaatcc
    15061 aaagcaacat taacatcctt gcttgaagat ataaatcggt tttgttgaac catatcttcg
    15121 ccaaatattc gttcaaattc tttttgcttt ataatccatt ccacgattta ccattccgtt
    15181 gtgaacaggc agtttttcca agccacgttc tattaacgtt ttagccgctt ggttaaattc
    15241 ggtaagcgta ttttttccca tctgcttgtt caattgccga taattgctac ctgctttcgt
    15301 tggttggggt ccaattcgag aatggagccc ggaaccaaca gaatataact gcgaactact
    15361 cccgctatgt cgcggtagtt ttccttgata cgaaaatagc tctcgagcat ttttctatcg
    15421 ggactgcagc gaactgataa atcgaaatgc attccgcaat gttctgtaat gcaaattaaa
    15481 aaaccaccaa aatcccggga tactacaaat gtgttctcag aaacaaaaac tattgaaatc
    15541 caaaatatta caatttcaat cgactgaatt tgactaccaa actaaccaaa atcagtcttt
    15601 ttttactaaa ctttaacgtt atgttattca tgtttgcaaa gttgggtgaa atagtcaatt
    15661 gttaaacagc aagtaatctg cattttttta aaaaaaatta atgttgtccg ataaaaataa
    15721 aggagcaaat agtgtcgctc ctacggagct ctaatgatat ttgtaactta ttgactacca
    15781 taatatcgtc ccgatgggac ttaagagaaa gctgcattgc agcgaaatta tggtagaaaa
    15841 attcaaccca cataatctaa gccccattag gggcgatatt atagtaagac attttctata
    15901 atggttttca ctaattttta taaatgttta ccgaacaaca atgaaaaaaa taattttttc
    15961 ttttgagatt aatcctatac ttttgtgata aagaaaaatt tactttataa atatgcataa
    16021 ggaaatccaa attactttac caccggaaaa agtaaataat aaatcagtac tcgaaaaaga
    16081 gataatcaaa attataaata tagagataca gcagattaca agatggagaa tcattcgcaa
    16141 atccattgat gcccgttctt atctaccaaa attcaacttg tctgttgaag tttattggga
    16201 cgaaatccca ccggaagaca cagcctataa ttttcattat aattttgttg gcaacaaacc
    16261 atcggtctta attatcggtt cggggccggc agggcttttt gcagccttaa aacttataga
    16321 acttggcctt aaacctgtta tattggaaag aggcaagcaa ataaatcaaa gaaaaaaaga
    16381 tattgcttcc atttgtcgaa atgagtgtat tgatccaaat tccaattatt gtttcggaga
    16441 aggaggtgcc ggaacctttt ctgatggtaa gctttacact cgctcaaaaa aaagaggaga
    16501 taaacttcat gttttacagg tttttcatca tcatggcgca aatgaaaata ttctttatga
    16561 agcacagccc cacattggtt ccgacaaatt gcccttaatc gttgagaata tatgtaaaac
    16621 cattattgat tgtggaggag agattcattt tgaaacaaga attaccgata ttttattaaa
    16681 aaatggacat ttaacaggtt gtaaaacaac tgataataat atttttgaag ctgcatccct
    16741 gattttggct accggtcatt ctgcgcgcga tgtttatgaa atgctttatc atcaaggaat
    16801 tctgctcgaa aataagggtt ttgcaatggg tgttcgtgtg gaacaccccc aacaattgat
    16861 tgatagtatt cagtatcata aaaagtatcg aggagatttt ctccctccgg ccaattatca
    16921 gttggttgaa caagtggagg gacgtggtgt atattccttt tgtatgtgtc cgggaggaca
    16981 tattgtgccc gccggaagtg gaacaaaaga aattgttgtc aacggaatgt ctgcatccaa
    17041 aagaaattcc ccctttgcca attcgggtat agttgtcgaa ataaaacctg aggatattcc
    17101 ttcagacttt tcaggttgtg ggccacttgc ctcacttatg tttcaaaaac atttagaaaa
    17161 attggcctat atcaataatg gtggtttgaa tctgactgct ccggctcagc gattaacgga
    17221 ttttgtgaat ggaaaacttt ccgacaattt gcctacttgt tcctatcttc ctggtttgat
    17281 ctcttccccg ttacattttt ggatgcctga ttttttgact aaacgtttac aagaaggatt
    17341 taaaaaattt gacaaaaaaa tgcacggatt tctcacccgt gaagctgtta ttgttggtgt
    17401 agaatcgcgt tcttcctctc caataaggat accgcgtgat aaggaaactt atcagcatcc
    17461 tcaaataaaa gggcttttcc cctgtggtga aggttcaggc tatgccggag gaattacctc
    17521 ctcagcttta gatggagaaa atgctgccat agctgtttat aaaagtagat tatatcataa
    17581 ttagttccat tttgtttgcg actgaaatta ttgaatagct ctcctctgga tttttttcta
    17641 ttctatctat ctatttaatt aaatatcaat aaaatagaac aaataagtgt gcttatgttt
    17701 agaaaaaagt atctttcggt ttaaaacgcc tcaaattacg ataggaaaaa tcgtttataa
    17761 gcaataaagg aatttattta tttatttctt tcatcagaat ataatttatt ttttaatggt
    17821 ctgatggaac ccacatgtct gaatattata ttcattcgtg tttgtgtatg caatacatat
    17881 gggtttcata atcgaaattt cattgaacat aaactacgaa ttgttgccgg ttggactaaa
    17941 aatggagcga agttaattgg agaacaattg tgtttttatt atctgataaa aaacaccgac
    18001 ttcagccaca ggatttcggg ttaaaataaa taaaatgaag gtaaaaatta tttttctccc
    18061 attttttaat gtacctattg gttctggctt aatttttgat gaaaattttc attcaaaatt
    18121 ctgaaccatt ttatggctta attgataatt gaatgattca atccttaaat gttaccatga
    18181 ttttttgaat tgccatcctt gctgtaacgt aaaagtataa atttaaaatg aaatgctttt
    18241 gataatttat tttaacaata ttgtacagtc aaatttttat atagatattt acttggaaac
    18301 tattaaattc taaaagtttt taattacatt caattaaatt attgataaac agtctcttat
    18361 atacaaaaaa ataatattta actttttgtt attgtttttt tgcttatgta cgatgatttt
    18421 ttaattaatt ttgcaatgaa aattttttat ttcgcaaaat gaaaaatact gaagaagtta
    18481 tacttcaaaa atcattggat ttgtttattg aatttggtat cagaaatgtt tccattgata
    18541 ccatttgcaa tgagctgaga atttcgaaaa agactttcta cagttatttt aaacaaaagg
    18601 aagatttggt ggacgcaata ttgacttatg aagaaaaaat caatattgaa gaaatgaaaa
    18661 aaagattcga tgataaaaat gcaattgaat cacttatctc cgttttgaaa gaaattaaga
    18721 aaaacatcga ctgccaacct aaacttatgt ggatggacat aaaaaaatac tatcctaaag
    18781 tttacgaaaa gcacgaaatg cgcaggcgtt ctattatcag aaaagatttt gagaagaacc
    18841 ttcaacaagg agtaaaagaa ggattttatc gagatgattt agacattgaa ttgctttcga
    18901 tgctccattc ataccaactg aaaaacataa tgaccaccat gagccaatac cccaaaaaat
    18961 attcaaaaaa acggatcgtt gactttttca tagacattat gatacacatt attgccaatg
    19021 aaaagggact tgaatatgta agaaaaaact attatgaaag tgaaccttaa gttttaaatt
    19081 ggtataacca aaaaataaat aaagaatatg acagcaaatt cattaagaaa atcaattttt
    19141 attgttcttt gtgtaatagc gcttttgcct ttctcactca aagcgcaaga tacattgatt
    19201 gtttcactcg ataaggcgat agaaatagcg ttgagtgaaa gtccgaccat taaggttgcc
    19261 aacaaggaaa ttcaaagagt ggattactcg agcaaggaaa aaatttccgc tttatttcct
    19321 gtcatatcgg cttcggggca atactccaga gctgtaaaaa aacagaaatt gttttttaat
    19381 attcccggta tgccggcacg tcctgacgga attgaagtag gacaggacaa tactttcagt
    19441 ggaggaattt cggctgccat gcctttaatt gcgcctactc tctgggcaac cataaaaatg
    19501 tcggagcttg atgcccagtt ggtaatggaa tctgcacgtt cttcaaaact ttcacttgtt
    19561 aacgaagttt caaaagctta ttatgctatt ttgttggcgc aggactcgta cgatgtattt
    19621 aaaaaaagtt acgacatagc ggtagaaaat gcaaaaatta tagaaaacaa atacaaacag
    19681 ggaagcgtat ccgaatttga atggattcgt gctgacgtgc aggtcaaaaa tgccaatgcc
    19741 aatttggttt cagcagaaaa tgcagttaat ttgagtgttt taagactaaa aatgttgatg
    19801 ggaattgaca tgtttatcgg tttaaaagtt gaaggaaatc tatcaaacta tgaagccaac
    19861 aaatatgcag atgtgctatc cattgacacc actaccctga aaagtaatag cgatttgaaa
    19921 caattcgata tcaaagccca gcaactcaaa caaagtttga agattcagaa atccactaat
    19981 cttcctacct tatcggcagc tatcaattat cagtatatga gtatggctga cgattcgata
    20041 gcattttcca attatcattg gttccccact tcaacggcag cactttctct ttctattcct
    20101 ctgtttcaag gaggaggaaa actctttaag caaaaacaaa ttgagattca ggtagaagaa
    20161 atgaaatatc agcgcgacaa tctcaggaga ggtttggaat tacaagccat gtcgtatttg
    20221 gataacatta atcagtcctt aaaagtgata gaatcgaaca aggaaggatt gcgacaggcg
    20281 gagaaagcac tgagtatttc acaaaaacgt tatgaagttg gagctgcaac ttatctcgat
    20341 ttgtccaatg ccgaattggc ctatacccaa gcaggcttat cttataatca gtctatttac
    20401 aattacttgt ccgcaaaagc cgatctggaa aaactacttg gcaatcagat tattttctaa
    20461 aattaaaata taatacagat ttcaatttat aataacaacc aaataaatga cattaaaatg
    20521 aaaaggataa ataaattagg gatattaatt atggccttaa caattgggct ggtgtcatgc
    20581 ggaaaatcag gaaaaaaaca gcaaggagaa gaagaaattc caagtgttcg ggttcagcaa
    20641 gtaaaaatgc aggatgtaga acaaaccggt caatttacgg caactgttca accagaagtt
    20701 aaaaacaata tttctccctc cacaccaggg aggataagaa aaattatggt tgaagttggc
    20761 acttctgttt caaaaggcca aaaattggtt cagatggatg ctgccaattt gtcaaacctc
    20821 gaaattcagg ttgaaaatta tcagcgaatg tataatcggg tggcagaact tttcagcgtc
    20881 ggaggtgctt cacagcagga actcgacaat gccaaattgc aattggatat agctaaaacc
    20941 aatttgcaaa atttatccga aaatacatat ttgctcagcc cgacagcagg tgttgttaca
    21001 gcacgtaact acgacaatgg tgattttttt aacggacaaa tccccattct taccgttatg
    21061 caaattaatc cggtaaaact actgatcaat gtatcggaat caaattatgc acaggtaaaa
    21121 attggaatgc cggctaccat taaattcgac atttttgaag gagaaacctt tcagggaaaa
    21181 atcagtttga tttatccaac tattgatgaa cgcactcgat cctttcaagt agaaattaaa
    21241 atgaataatg agaattataa aattcgtccc ggcatgtttg caagagttga aatcaatttc
    21301 ggttctgccc ggagaattat tgttccggat atggctgtta ttaagcaatc cggttcggga
    21361 gaatattttg tttatgtcta taaggacgga tcggtaattt atcagagagt ggaattaggt
    21421 aagcgcttgg gcaaagaata tgaaattatt tccggcctga atgatggaga cttggttgtg
    21481 gtttcaagtc agtcaaaatt ggcagatggt aagaaagtga atttacaaaa ataaaaattt
    21541 ttaccgaaaa aagttttttg aaaaacggta ttaaaataac ttagttcaaa atttaaaatc
    21601 tatgagttta tacgaaaatt cggttaaaag gcccataatg acatctctga tttttgttgg
    21661 agtaattatt ttggggttgt attcattaag caggctacct attgatttgc ttcccgatat
    21721 agagccaaat attttaatgg tagcaaccaa ttatacagga gcaagtgcat tggacattga
    21781 aaacaatgtt acccgtccgc ttgaaaacac attgaataca gttgaaaatt tgaaaaatat
    21841 tcgttccaat tcaaaagaaa acacttctgt tattattctc gaatttgaat ttggatacga
    21901 cttggatgta ttagccaatg atgttagaga caagctcgaa ttggtcaaac cctacttgcc
    21961 tgaaggagcc agtaatccgg taatttttaa attcagtacc gatatgattc ccattgtgat
    22021 gatttctgca acagcagata aaagtttacc ggcactttat aaaatacttg atgaaaatgt
    22081 agccaatcct ctggcacgta tcaacggtgt tggttctgtt tccatcaacg gagcccccga
    22141 aagggaaatt caggtatatg ttgatccggt caaacttgaa gcttatggtt taactgttga
    22201 atcaattggt cagaatatcc gtcttcaaaa tatcaacact ccaagtggtt cgatggatat
    22261 aggaaataag acttatactg tcagggtaca gggagaattt aaagacccta ccgagctgag
    22321 taaagttgta gtgggaagtt acaatgggaa gaatgtttat ttgtcggatg tggcaataat
    22381 aaacgacgga cacgaagaac gaattcagga aagttacacc aatggcgttc agggagcaac
    22441 tatcataatt caaaaacaaa gcggtgcaaa tacggtggaa attgccaatg ccatcagagc
    22501 caaattaccc caaatagaaa aaaatcttcc ttctgatata aaactgaata ttattaatga
    22561 cacttcagag aatattaaaa acacgatagc cggtctggaa gaaactattt tatatgcttt
    22621 cctctttgtt attttggtgg tgttgttttt cttgggaaga tggagagcga ccattatcgt
    22681 cattcttaca attcctatat cacttatcgc cgcctttatt tatttgagcg gcacaggagg
    22741 aaccctaaat atcatttcgc tcagttcatt atcgattgca ataggtatgg tggttgacga
    22801 tgctattgtg gtacttgaaa atattacaaa gcatattgaa aaaggaagta gaccgaatag
    22861 cgcagcaata cacggtacca acgaagttgc aactgctgtt gttgcttcaa ctctcacgat
    22921 agtcgccgtt ttctttccct tgacgctggt agggggattg acagggattc tttttgccca
    22981 attaggctgg atggttacta ttattatggt agtttcgctg gtttgtgcct tgtcgcttac
    23041 tccaatgttg agctcgaaaa tgctgcgact taatcctcac cattctcgta tgtttactgt
    23101 cgtttattca ccgattgtaa aatttctcga caatgtggat gcgtggtatg ccaatatagt
    23161 caactggtcg gttcgtcaca gatggcaaac agttgctatc gcagcaggat tttttgtgtt
    23221 gagtttgatt cccttaatcg gtataggaac agaatatatt cctgctcagg acaattcgcg
    23281 catcagtgcc accatagaac tacctgttgg tacaaggatg gaaatctcta agaaaacagc
    23341 agaaaatctt acagaacttt ggagagaaaa atatcctgaa attcaaacca ttaattattc
    23401 cgtagggcag gccagcagtt ccaacatcat tcgttcaatt cagaacaccg gtagccatat
    23461 catttctttt accctgcgtt tgtcggatac gaacgaacga aatagaacta tttttgaaat
    23521 tgcagacagt atgcgtgtgg atctcaaatc ctttttggaa ataaacaaag caaatgtaac
    23581 tgcaggtgga gcaatgggaa caatgggagg acagtcgacg ctggatatag aaatatatgg
    23641 ctacgatttc aacgaaaccg ataaagtggc aatggaatta actaacagaa taaacaatat
    23701 tcagggactg gttaatacaa caattagtcg aggtgattac tcacccgaat atcaagtaga
    23761 atttgatcga gataaactgg cacttaacgg attgaacatt ggaaccgcat ccaccttttt
    23821 gcggaacaga attaatggtt taacagccag cctgtatcgt gaacaaggcg atgaatatac
    23881 catacgtgta gtatatgctc ccgaatacag acagagtata gaagatgttg aaaacatatt
    23941 gatatacaat actatgggac aagctatacg gcttaaagat ttggggaaag tgatagaaac
    24001 cttaacacct ccctatatag aaagaaaaaa tcgtgaacgt gtgattacgg tttcctcaac
    24061 tatttcggga accactctcg ataaagcagt gcaagccatc aacacagaac ttaagaatat
    24121 ggaaatacca acaggagttg acacacagat ttccggttct tatgaagatc aacaagagtc
    24181 atttgctgat ttaacaactt tgttgctgat tgtcatcatt ttggtttaca tagttatggc
    24241 atcacaattt gagtccttga cttatccgtt tatcattatg cttgctttac catttgcatt
    24301 ttcgggagtt gtgctggcat tgtttttaac gggtggtacg ttaaatttaa tgtccatgat
    24361 aggaattatc atgttggtcg gtattgtagt aaaaaacgga attgttctgg tagattatat
    24421 caatttaaat cgtgaacgcg gcatgggaat tgtcagggca attgtcagcg gaggtcgttc
    24481 ccgtttgcgt cccgtattga tgacaacttt tactactata cttggtatga ttcctttggc
    24541 aataagttca tctcaaggat cagagatatg gaaaccaatg gccataacca ttattggtgg
    24601 cttaacagtt tccactattc tgactttact tattgtgcca acgttgtatg caatttttgc
    24661 cggattcgga attaaacgca cttacaacaa gtacaaaaag ggaaccaata agttgcaaca
    24721 atccgaaaaa taataattta attgaatttt tacctgacca agaaaatata aaaaagtagg
    24781 taaagatttt aagttaaaca aaatgttttt atacaattca aaaaagaaga ttgagattgg
    24841 actgggtttg gttaagttct catttaatat atctggtcca atcccgattg ttaatcagaa
    24901 aaaacaatat ttatgaaagc aatatttata gttttcgatc aaggttttta tcagcagata
    24961 ctcgaatcgc tttatataaa tcatcatgtt aaaggattta ccgcttggga aacagtaaaa
    25021 ggacgcgggt ctaaaaccgg cgaacctcat tatggaagcc atgcatggcc atccctgaat
    25081 tcggcaatta taactgtagt ggctgaagaa aaagtaaaac ctatactgga tgatctaaaa
    25141 gctcttgatg caacttcgga tgtgatggga attcgtgcat ttgtttggaa tattgaagat
    25201 ggtatgtaat tcttgttttt gcttgaattt tattttttat ttttactgca ttgctcaata
    25261 agtgaaaagg cgctctaata atgagtgcct ttttaataat agaaaattga ttttatcgag
    25321 atgaaattac agatacgtat ttttcagtat ttgcaccaac tcttttattc cttcgcttgc
    25381 tccttttttt ataaaagttg tatttgatgt atttaccatt acttcttttg gatcaatgag
    25441 ataaattggc gtaccgggtt tgacgtagcg aattaatcct gcagcaggat aaacttgcat
    25501 tgaagtacca ataattataa atatatcggc ttgagaaacg aggtttatgg ctttttctat
    25561 ttcggggaca ggctcaccaa accaaactac gtgcggtcgt aattgataac ctttaggaca
    25621 tttatctccc acatgaattt cgatattttc agaacttaat tcatacactt catgtcccgg
    25681 accggtggag caaactttca ttaattctcc atgcagatga ataacacgac tgcttcctgc
    25741 tttttcgtgt aaattgtcaa cattttgagt aataatatac acatcaaaaa aattttccaa
    25801 catggccaaa gcttcatgtc cttcattggg cacggtttta agcaactgtt tccggcgttc
    25861 attatagaaa tttaatactt tctcgggatt tttcttgaat gcttcagggg tagcaacttc
    25921 ttctatacga tattgttccc ataatcctcc gctatctcta aaagtagaca agccactttc
    25981 agcgctcatg cctgcaccgg ttaaaactac aattttgttc attgtttgtc tgtattataa
    26041 ttgcagataa atgtgagatt tttatcaaaa aaagttgtta aattttattg tatcgatatg
    26101 tttcgataat ttgtataaaa acagcaactt ttgcagaaaa atcagccatt aggggtttct
    26161 aaatattgaa ataacaatat ccgcctttta gattttgatt gtccatttct ggcctttttt
    26221 tgttgagtcg gttttgccct tgtagcgaca atcggactga tgtagcgcac aaacaaattg
    26281 gggattaagt tcttcattct gccttgctat cacagctttt tcttgaccga ctctatactg
    26341 ctgtttaaaa acaaactaat tttaaaaagc ttttcggagt gaatgccata attttttaag
    26401 tgataacagt acaatttctt tcgccaaaaa tgcaagttcc caacctgacg attgtactgc
    26461 cttcttctat tgcaatctgg aaatcattag acatacctat tgaaagttct cgaaaggatg
    26521 cttcgttact gaaaaatgaa tctttcagtt ggtcgtatag taattttaaa ttcctgaatt
    26581 cctgtcggat ttgagtttga ttatctgtat aggttgccat gcccatcaac ccacaaattt
    26641 gaacattgtg taaatttttc cacccttcgc ccttcaacat ttctataagt tgttcaggta
    26701 gaaatccgaa tttagatgat tcacgagcaa tatggatttc caacaagcaa cgaacaactc
    26761 ttccaatttt acgggcgtta cggtcaattt cttccaaaag tctgaaagaa tctacggcat
    26821 gaatcaaatc cacaaacgga acaatatact tcaccttgtt ggtttgaaga tgtcctataa
    26881 aatgccattg aatatcaccg ggaagagact tttgtttttc acaaagttcc tgtaccctgc
    26941 tttctccaaa aattctttca cccaagtgat acgcttcgag tatggctttg ttgggttgaa
    27001 acttggaaac acataccaat tgcacgtttt cgggaatttg aacacgaatt ttttttaaat
    27061 tcttttcgat agacatgttt atgtattctt ttgtaaattt ttccttattg gaaattcgaa
    27121 gtttcttgaa caactttttt ctcttccgcc acataaggat aaacttccat tatgggcgtt
    27181 tcggaaacgg aaactaattc ataatcgacc aaagaatttt tcatttctga agtaagaact
    27241 tctatagctt cttttacgga agtagcctga acaagcatag taacagccgt acgcttttct
    27301 acactttttt cttcatccaa tgaaataaag aaaactttgc aacggtacca tctgtcaccg
    27361 ccatcatgtg caaaaatttc gttaatgcgc gctctgcgaa tgttggccac caaaaaatct
    27421 ccagaaatat aaggttccat ttcctgaata atccgctttt cagcttccgt aaaacttaaa
    27481 gcatctatta agtatgtttc gttcacggta agggtttttc cttcttcaat cactttttcg
    27541 tatttgattt tgcattcgaa ccaagtgtgc atcttaacta attgtttaaa tatgaataaa
    27601 tttagttatt aaattgctta ctgttgcagt ttctacaaat ttaagggata aattttgatt
    27661 ttcatcagaa tataatttat tttttaaagg tctgatggaa cccacatgtc tgaatattat
    27721 attcattcgt gtttgtgtat gcaatacatg tgggtttcat tccttgttaa ttcattaatt
    27781 tttttcccaa aaaagcaatt ttttcctcta tcacaacttg ccattgcttt aaataacatt
    27841 tgatttacgt aaattggatt tttacttaca aaaaaaagat gtaaactttt tacagcatac
    27901 atctttttgg ataagtttaa tttacttttc tataaattag tgaacgataa cattgggatt
    27961 aatctttttg atcaatcctt tcaagacatc tccgggtccc aattcaataa attcatcagc
    28021 tccatcagta atcatgtttt tgatggtttg tgtccatctt acgggagctg tgagttgagc
    28081 aattaagttt tgtttgatat taacgggatc ggtttgtggg taagcattca cattttgata
    28141 aacagggcaa ataggaatag aaaaggtagt ttgactaata gcttcgctca attccaatct
    28201 ggccggttcc atcaacggag aatgaaaagc tccacccaca ctaagtttga gtgctcgttt
    28261 cgcacctctt tccttaagca tttcgcaagc catgtccaca ccttgcaatg taccggaaat
    28321 gacgatttgt cccgggcaat tgtaattggc aggcaccaca atcatgtcct taatcgaatc
    28381 acaaacttct tcgacaattt catcatccaa cccaataatg gcggccattg tagattcttc
    28441 gagttcgcag gctttttgca tggccacagc acgtttcgac accaaagtta atccatcttc
    28501 gaaagtcagg gcacgagctg ccaccaatgc tgaaaattcg cccaatgaat gtcctgcaac
    28561 catatcggga ttaaattctt ctcccagtac aaaagccgaa attaccgagt gcagaaaaac
    28621 ggcaggttgt gtaactttag tctgtcgtaa gtcttccgga gttccttcaa acattaaatc
    28681 ggtaatacga aatccaagta tttcgttagc cttttcaaaa tattccttcg ccagagtaga
    28741 ctgttcgtat aaatctttgc ccattcctac aaattgagcc ccctgacccg gaaaaacaaa
    28801 tgctttcatg tgctttctgt ttaataattt taaattttta aataacgata taattgaaaa
    28861 taaacttatt aaatataaaa ctttcttcaa acggactgca aagttaataa aaaagtaccg
    28921 aaaagaaata aattcgcact tctatgctga tttaacagtt cgaatgattt ctgctacttt
    28981 tttgatctat aaaggaaata taatcaaaaa aaaattattc cttattccac acaaataatt
    29041 ttctcctaac aaattctatt cacaaaattc actatttttg tcatctaaat aagtttaaga
    29101 gcaagaaata taattttctg caatcaacac cataatttct ctacaatttt atgtctgcaa
    29161 ccggcaaaaa aaaattgaaa atacttcgtc gaatttttct gaacaagtac acccttgttt
    29221 ttttgatttt tgccggatat gtaacttttt tcgacaaaca caatctgatt tcacgctgga
    29281 aaatttacca aaaaaacaaa gaactggaaa aagaaattaa atattacgat gaaaaaataa
    29341 aatcggatcg taccaaactt ttcgagctca aatccagtaa cgaaaatctt gaaaaatttg
    29401 ccagagaaca ttatttctat aaaaaagaca atgaggatgt atttattatt aataagaaag
    29461 attcaaataa aaacagataa aaaaacaata aaattgcttt ttgaagaaat atctttcgag
    29521 tatttcacaa ttcaaaacca aatgtttaaa aaatttttct gcatacatct atgaaaaaat
    29581 cgaacaattt tttcaaaacc atctatcaaa aatatcccta cttatttgtt ttgtttctca
    29641 gcttgctgtt gactattccg tggactatgg tgagcgattt ttacaccaaa ggcgaaccac
    29701 gagaagcttc tgtagcacag tgtattctca atacgggcaa ttggattcta ccaaccgatt
    29761 atgcagacga aacagcgtac aaacctccct tgatgcattg gatgatcgcc ttattttcac
    29821 ttccggccgg aaaagtatcg gaaaccacag cccgcttgcc ttcagcattg ggattgattg
    29881 gtattactgt ttcctttttc gtgtttcttt ccaagcgcac atcgaaaaaa agagctttac
    29941 ttgccacact tttaatgctt acaagctttg aaatgcatcg ttcgggcatt gaagcacgag
    30001 ttgacatgct actttcattt tttatgattg catcattaat ttgtttttat atttgggaag
    30061 aaaaagggtt gcgaaaattt ccttggttta tttccctgct gctggccgga gcttcgctga
    30121 taaaagggcc ggtcggcatt gttttaccgt gtttggtttt tattgtttac cttttgctgc
    30181 ttcagaaata ttctttctgg aaaattatct ggaaaaattt tcttgtggct cttccggccg
    30241 tttttatcct tttaggttgg tattatttgg catatcggca aggaggagaa aaatttctga
    30301 atgttgtcta tgccgaaaat ttcggtcgtt ttttgggtca aaaaagcgaa acattggata
    30361 ttgcatacaa tcttggccac gaaaatcctt tttggtatta cattcctgca attctcacag
    30421 gttttttacc ctggtcactt gtggcaattt tcagtttgtt tgccgtttcg tacaaatagc
    30481 tttttaaaaa attgcaattt cgaaaattga aagaatcatt caagaatttg aaaaatgcag
    30541 acaaactggt tttattcagc accataactt taattgtggt tgttggcttt tactctatac
    30601 cgagttcaaa gcgaagtgtt tacataatgc cagtatatcc gtttgcagcc tatttgcttg
    30661 ttcttcttta cgaatgggca atgaagcgaa aacctcaatt tatcaattgg acagcaaaca
    30721 tcattttcat tttagtggga tttttattac tgttaatggc cttctcccga tttgtaaatt
    30781 taagtgccat aacctcccca ctcataaaag ataaaacctt gcatcacgat atcggactat
    30841 attcaaaagc atttcaacat cccggagttg caggtgttct gttctggctt tttctggcaa
    30901 cagcttttgt ttggtatctg aaatggaaac ggacaagaat tttgcagaac attctttttg
    30961 cttccatagg attatttatt ggcttacaag tttttctgga aggatttgcc tatcctattt
    31021 ataaaaatgg ctattctgtc cgtccttttg ccgaaaagct tcaaaataaa tattctttaa
    31081 aaaacaatgt ttttgtaatg aacaatttga aggaatatcc aaatctatac gcactaaact
    31141 tttatttggg caatcggttc aaaaactttg aaaaagaaaa accggaagaa ggatattttc
    31201 tgactggaga aaaaagtttg aaaaagataa gaaaagttta tggtacaaat taccaattca
    31261 ctgttttgga aaactcaggg aacagattta atgatattaa agatgtgatt cttttttgcc
    31321 gcttgaaacg aataaaataa accctatcct gataatttga aaacacaatc gggatagaaa
    31381 catttcttct gtcaaaaaaa ctcattgaga aacctccatg tattgcatac acaaacacga
    31441 atgaatataa tattcagaca tgtggctcca tcagaccatt aaaaaataaa ttatattctg
    31501 atgaaaagtg taaacgtaag ctatccttta gaaatattta taaaaaaatc caaaccattt
    31561 ttgaagttcg gattcttttt ttgaaaacgg aatcagtttg acagttctat ttattcagca
    31621 tatcattcgc tgatttttct accaacaaac aataaagacc actttgcccc atcaatcaag
    31681 attactaccg actgaaattc caaaggtaaa tggtaatttt tctgttgcag taggttactc
    31741 gacaattttt ttattcagga aaaggtgagt aaaaaaacca acaatcatga gaatacctgc
    31801 agttcctaaa ataagattag aaggatgtcc tccgaattga taatatgcaa gcaatgcaac
    31861 acctatcaaa atgattatag aaccaagata aggcaaaaaa tctttcattg taatgatatt
    31921 ttttatgttg aaacttcaaa ctgcaaatta aataattttt tgtcagctaa tcaaattatt
    31981 tttttaaaaa ttgaaaaact cactacaaaa aattggcaca accctccatc attaaggaaa
    32041 aaaccgcaga tctaaaattt ttaaattttt ctcggctaaa ttcttgaata aaatcagtag
    32101 atttgtattt gatttcaaag agactgattt ttttacaaaa ttctttatat tagcatgata
    32161 gaccaagcca ccattgacag aattatggaa gctgcacaaa ttcaggatgt agtttccgat
    32221 tatgttacgc tcaaaaaaag aggcgtaaac atgattggtt tgtgtccgtt tcacaacgaa
    32281 aaaacgccat cgtttaccgt ttcacctgct aaaggaattt tcaagtgttt tggttgcgga
    32341 aaaggtggaa acgctgttca cttcataatg gagcatgagc aactgtcgta ttacgatgca
    32401 ttgaaattcc ttgccagaaa atacaacatt gaagtgcagg aacgtgagct gaccgatgaa
    32461 gagaaagaac ttcacaacga acgggaaagt ttgctgctgg taaacgaatt tgcccagaaa
    32521 tatttttccg atacgcttta tcatcacatc gacgggaaaa ccatcggtat gagttatttt
    32581 caggaacgcg gtatccgaga agacatgatt cgtaaatttc agttgggtta ttgcctcgat
    32641 cagcaggatg cttttacgca aaaagcttta aaagccggtt acaataaaaa atatcttgta
    32701 aaaaccggac ttaccatcga aacggaaaac aatcgcctta ccgaccgttt tcgcggacga
    32761 ctgatatttc cagttcattc cctttctggc aaaattattg ctttcggcgg aagggtaata
    32821 aaaaaagacg acaaaacagc caaatacctc aattcgcccg aatcggaaat ataccacaag
    32881 ggaaatgagc tctacggaat gtattttgcc aaacaggcca tcgtaaagga agaccgctgt
    32941 ttgctggtgg aaggttatac cgatgtaatt tctcttcatc aaagcggcat tgaaaatgtc
    33001 gtagcttctt cgggcacgtc gcttacgccg gctcaaattc gtcttattca ccggtttacc
    33061 aacaatgtta ccgtgctcta cgacggcgat gctgcgggca ttaaagcttc gttgagagga
    33121 atcgatttgc tgctggaaga aggcttgaac ataaaggttg ttttgttgcc cgaaggagaa
    33181 gatcccgatt ctttttccca caaactcaat gcagtggatc tcttgaattt cattcgggac
    33241 aattcggtgg attttatccg cttcaaaacc aaacttttga tggaggacgc agcaaatgac
    33301 cccatcaaac gttccaacct gattctggac atcgtgcgaa gtattgccat tattcccgaa
    33361 ccgattgtga gagccgaata tgtgaaggaa tgcagcaatc tgcttcaagt gaacgaagaa
    33421 attctttacc atgaaataaa ccggcttaaa agaaaagaaa gcgaaaaaaa acttggacaa
    33481 agcaatgttt cggcttcttc taccgaacca aaaccacaac ccgtttctcc ccaggaaatg
    33541 ctgaatgccg aaaaccagag gtttgagcag gaagaaagaa atatactcca agtattgatt
    33601 aaatatggta aatcggcatt gtactattct gacgaagaaa accgttcacc gattactgta
    33661 gaagatttta ttattgaaga cctcgaacag gatcagcttg aatttgttca tccgctctat
    33721 gctcaaatgc ttcaggaata taaggaacaa tgcttgaaaa aaaatattaa cgttcaaaat
    33781 tttttcctgc atcatattca tccggaaatc agcaaattga cggcagaact gatttcagaa
    33841 aaatacaccc tcagcaaaat ttacaaaaaa acgagaatgg acgaacccga gatcgataaa
    33901 gtgagggaat ttgccgaacg tgccgtgctg gaatacaaaa acagcattgt tctctcgcag
    33961 attaagcaaa aattgctcga aatgaaaata gcggcagaaa accacgacga tgagttggtt
    34021 cgtattctga tgattcaaat cagtcagttg gaagaaatta aaaatcaact tgcaaaaaat
    34081 cttggagaac gcattgtcat taaattataa acagcttcat aaatgacata cgaagaattg
    34141 attcatgaca ccgagtacaa agaactcgca tctctttcac ataacgaaat aaaaaatttc
    34201 attgtcaacg aaattaccga aaaccaacaa tgggcacgtg ctgccaattt ataccaattg
    34261 acaggaattc tggctttcat gctgggaact ttcaaagcct tcatgcctta ttttgtccat
    34321 cgtcaagcct acggtttgct ttggctgctg gcaggcattg ttttcacctt taccattctc
    34381 atcgtttttc atgaattgat tcatgcgctg gcctattggg caatgggggc tcgtcatttg
    34441 ttttttggct gccagcccaa aaaattcatg ttttatgttc aagccgacaa ggctgtggtg
    34501 ggttaccgtc agtttaaaat aattgctttg gcaccaaccg ttttcatcag tttcctttcg
    34561 ctgcttggaa tgattatctt ttatcatcaa ccggcatttt atttttttat ttcgatattt
    34621 ggtttacaca gcctctttgc tgcaggagat tttgggctgt tgtgtttttt tcaaaaccgt
    34681 cccgaattgg aaattgttac tttcgatgtg aaggaagaag gtaaaaccta cttttatgga
    34741 aaagaaaaaa gaaaaaatat tgctgtgtag gctttgaaag gaaagcaatt ttttcgcaaa
    34801 ataatttaac gttatttgaa aatatcgtag aagaatgatt ttatttgatc agattgtttt
    34861 tgggcctgtt catagcaggc gtcttggcac ttctctggga atcaatttat tgcctgtaga
    34921 tgccaaaatt tgcagcttcg actgtctgta ctgcgaatgc ggatttaata cttccggaaa
    34981 gcacgccaaa ttgcctacaa ggatggaagt acacaatgcg ctggaaatga ggttgaaaga
    35041 aatggcaagc gacggacaaa tacccgatat tataactttt gccggaaacg gagagccgac
    35101 acttcatccg gattttgccg gaattattga tgatacactt gaactgagaa ataaatattg
    35161 tcccaatgcc aaagttggag tattgtcgaa cgccacacaa attcacaaac cagacgtttt
    35221 caatgcttta aaaaaagtgg atgacaacat tctgaaattc gacagtgctt tcgatcatac
    35281 tttacaactg ctcgaccgtc cggtaagaaa atacatcact gttcgctggc tgattgaaca
    35341 tttgaaaaag tttgaaggta aaatgattgt gcaaacttta tttattcgtg gcgaagtgaa
    35401 cggagaaaaa attgacaata cttcggataa ggaaatagaa gcatggctga aagcattaaa
    35461 ggaaatgaat gcaacgcagc ttatgattta taccatcaac agagaaactc ctttgaaaac
    35521 tttggaaaaa attccgttgg aggaactgaa tcgtatagcc gaaaaagcaa aaagctacgg
    35581 atttgacgtt tctgtttcag gataattacc aaagccgaag atgaaaatac tcgtttgtcc
    35641 tttaaactgg ggattgggac atgctgcacg ctgtgtgccg attattcgtc aactgattac
    35701 agaaggcaat gatgtggtga ttgcagcaga tggtttccca ttagaatttc ttcaacttga
    35761 gttccccgaa cttcgatttt tgaaagctcc tactcaaaat gttcgttaca gcacgggaaa
    35821 ttctcaaatc tgggctatgt ttaaaagcat cccgcgtatt tttggcaata tttttcaaga
    35881 tcattgttgg cttcatcgac ttttaaaaca ggaacatttc gatcgcatta tttccgacaa
    35941 ccgttttgga tgttggagca aaagaacgta ttgcatttac attacacatc aactgatggt
    36001 aaaaatgccg cccgaattgc gcttgctgga accgattgtc tggcgacttc atcgcttttt
    36061 cattcatcgt tacgacgaat gctggattcc cgatttggcc ggagatgaaa acttttcggg
    36121 agatttggca catcgttatc ctttacccga aaatgcacat tttattggca ttctatctcg
    36181 ttttcaacaa ttgaaaaata ttattccaaa ctatacttac gaaacggtga tggttctctc
    36241 cggtccggaa ccgcaacgta ccattttcga gaaacaactg acagaaaaat tcagcaaaga
    36301 cactgtcaaa tcgttgatga ttcagggatt gccacaggaa aaacaaactt ccgtacaagt
    36361 tggcaactta accaaagttt ctcatctttc cactcaagaa atggcgtcct tattagcagg
    36421 cgcaaaaaaa attattgctc gttcgggata ctcaaccatc atggatttgc aagcactcaa
    36481 ctgcttggac aaagcaatac tcatccctac tccgggacag accgaacaga tttatttggc
    36541 agaactgcac aaaaagtgca gataaaactt caatccattt tgtagccaac aatcgatttt
    36601 ctgatttcaa tcagttttat gagaaaattt ttaaccatca aagaataaga taattggctc
    36661 tttcatccat ttaaaaaaat caatatatac atcaaaaaaa cggaagcaaa aaaattttta
    36721 cctccgttcc aaataaatgt tttaaacttg ttgtctttaa atcagttttc gattattcgt
    36781 gaaacaaaat tttgcacttc tgcataagaa atgttcatga ttccctgaat ttctttttcc
    36841 attagaggtg caagcattga aatattcatt tgagacacgt aagtatttcc atccgatttt
    36901 tcgtaaattg aaattctaca cggcatcatg ctcgaaaaag tgcgccattc atcttttccc
    36961 aaaatggaat aggcatgata tggagaacac agttccaaca cttttacagg taaaacttct
    37021 ttattgtttc ttgccatagt ttcctgcagg tcatgcacat aaaccagtct ccaacctcct
    37081 tcctgaatag catttgtcaa ttcagagagg gttttgtgga agctgaattt actcttcttt
    37141 tccaaaaaaa attcttccat tcctttcggg ttgtcttatt gttttacttt ttctttctta
    37201 agcaatacct catcaatggc atttttcagc acatcttttg gcaaagcgcc ttgagccatt
    37261 tgcggattct cattcatagg acagaaaagt aaagttggaa tgctacgaat gccaaatgct
    37321 gcagccagct cctgttcctt atccgtatca attttataaa tataaatctg tccatcatac
    37381 tctttagcca gttcttccaa tattggagcc actgccttgc aaggtccaca ccagtcggca
    37441 taaaaatcaa tcacgcaagg tttgtcgccc agatatttcc attcgttagg gctggcttcg
    37501 taattgacta cctttttcaa aaattcttct tttgttaaat gaattgttcc cattagtttt
    37561 tcttcgtttt tgttttgtga attattctgt ttgttttctc cattgcttcc cgcacaagcg
    37621 cttaaagcaa taaaagtgat cattaaaaat attccggtca attttttcat attcttctta
    37681 tttttggttt ttattttttt tgtcgaaaat tagttttcaa aacgtaaatt tacaagtttc
    37741 tcatcggatt ttaatttatc tcattatagt ggtaaattca ttttccccgt tctccgacgt
    37801 ttttaccagt tgaaacagag cacgactgcc cacagggact tagatggaac aaagcaatca
    37861 aaagcaaaaa actgccgatt atcaaataaa caatttctaa agtggagata aaagcaattc
    37921 ccaaaacaat cccaagtatc agttgaatca ttctgacgat attccactga aaaaatattt
    37981 ttttaatcct cctgatcgtt cgcattggtg tttttacttt tcactttaaa catgtcaaaa
    38041 aacaagtaac ccaaaacggc tccatataca atgctaatgt acggattgga actgatggga
    38101 caagttcccg atttacaacc aacaaaataa aaatacgcaa atcctccgat gctccccaaa
    38161 agcactccga ctactttgag ccaatgacgc gaaaggaatt ttttcaacat agttaatcga
    38221 aatttagttg gtgcactgac aagaacgaat acagtccaaa atttgaacta tttccttgtt
    38281 tttcaacgaa taataacaat tcttaccatc ccgacgacaa tttaaaatgc ctttggcacg
    38341 catatcggtg agatgatgag aaagcagcga ctgttcacaa ttcaattctt cacttatttg
    38401 cgaaacattc aattcctctt tctccgaaag cagcgaaatg atacacaaac gtgtagcatg
    38461 tgaaatggct cttagcttat aggctgcctc ttccatttgg tctatgttaa tattgttaat
    38521 ctgttccata tttcaaaagt tgtgcaaata tatgaacatt tattcatttg tctataataa
    38581 gttcagaatt tttaacattt actctattta tttccataat gttattaaaa caaatacatt
    38641 gtctctgatt tatggctttt gtttttaaag aaaaaagtat ctttgtattt taaatcagaa
    38701 attcatacac atgacctctt cttcaaccat aatatgtgaa aattttattg ccgagttgaa
    38761 tgaattcatt gaatatcaaa agccggacgg aatttttgtg ttggtggacg aaaacacaaa
    38821 aaagttctgt cttccgctcc tgcttcaatc ggaaaaacta aaaaattgcc aagttataac
    38881 cattcccagt ggtgacgaaa ataaaaacat ccataccgcc attgaagttt ggaattactt
    38941 atcatcacat ggcgcaacac gccgttcgtt gatgattaac gcaggcggag gaatggtttc
    39001 cgatttgggc ggttttgcag ctgctacttt caagcgcgga atcagctaca tcaatgtctc
    39061 caccaccttg cttggagctg tagatgctgc caccggtgga aaaacaggta tcaactttaa
    39121 ggggttgaaa aacgaaattg gcgtttttgc tccggctgac aaggtactga taaacgtgga
    39181 ttttttcaga acactggacg acaagaattt gcgttcgggc tttgcagaaa tgataaaaca
    39241 ttcgctgctc gattcgcgcg aacattggaa aaatattctg aaatttaatt tggatacgat
    39301 tgattatgaa cttttaaaac acagagtggc ggaaagcgta aaaataaaag aacaaatagt
    39361 cgaaaaggac cctaaagaac aaaatctgcg aaaggcattg aatttgggac atactttcgg
    39421 tcatgctttc gagagttttt cgtacagtca acatcaaccg gttttgcatg gctatgccgt
    39481 tatgtacgga cttgtatgcg aattatatct ttcacacgtg aaactgaact ttccgaaaga
    39541 agatttgtta aagctcgtta cacttgcaaa agaaaattat ggaaaaatct ctatttgttg
    39601 cgatgattac gaacaacttt atgaactgat gacacatgac aagaagaatg tttcggatac
    39661 catcaacttc actttattgt cagaagtggg gaaaattgaa ttgaatcaaa ctgctacaaa
    39721 aaaagaaatt tttgaatgtt tggattttta tcaggaaatg taaagtgact aaaaacaaca
    39781 ttacgaaaaa tgttagcatc aaatacaaaa aatgaaaaac caatgaaaaa aactttagta
    39841 ataggtgcca gcaccaatcc tgaacgttat gcctacaaag cagcgcaacg tttgctcgaa
    39901 aatggccacg aaatagaatt gctcggactt cgacccggcg aaattttcgg taaatccatc
    39961 gatacggaaa aaaaacaata tcccgacatt gatacggtaa ctttgtacgt aggtccaaaa
    40021 aatcagcctg aatattacga ttatatctta tcactccatc cgaaaagagt ggtatttaat
    40081 cccggaaccg agaatcctga atttgaagcc ctgctggaag caaacggcat tcaagcacag
    40141 gaagcatgta cgctggtatt gctgagcaca caacaatatt gaagaaaatc aaaaaaacaa
    40201 caagatttta aaacttcatt ttttcaacaa aatatttcaa ttttttatga aattagcatt
    40261 gattggttac ggaaaaatgg gaaaagccat tgaacgaata gcaaaggaac gtgggcacga
    40321 aattgtttgt atcatcgaca ttgacaattt gaatgatttt gaatccgata aatttaaaag
    40381 tgccgatgtg gccatagaat tcactcatcc cggcgtagcg ctgagcaact acagaaaaac
    40441 ctttgctgcg ggcattcctt tggtatctgg gactacaggc tggacaaacg aattacctgc
    40501 cttaaaaaag gaagtggaag aaaaaggcta cacattcttt tggtcatcaa attttagttt
    40561 gggggtcaat atttttatgg ccgtaaacaa atatctggct tccctgatga atcgtttttc
    40621 ggattacgat gtggaaataa cggaaatcca tcatattcat aagctggatg ctccaagcgg
    40681 aacagccatc acattggccg aagatatgct ggaaaacttg gaaagaaaga cctgttgggt
    40741 aaaagaagaa gcaaaacagc cccatgaatt ggccataaaa tcaattcgtg aagatgaagt
    40801 cccgggtatc cacattattc gctacgatgc ttcatttgat gccatagaat ttaaacatga
    40861 agccaaaacc cgcgatggtt ttgctctggg agctgtaatg gctgcagaat ttgccgtcgg
    40921 gaaaaaagga tggttaacca tgcaggacct gctaaaattt tgatattttc tccatttagg
    40981 aaaatcgaat gctaaaaagt tcaataattt ctcacagaac tgaacaaaac aataaaaatt
    41041 caaaaaactt aacaatttga tttatgactt ccgaacctca cacatcccgt ttccatcaac
    41101 ctcgtcgaca atggataaaa gctgccattt ggagtcttat ctacatttta ttcattgctt
    41161 gggtaggtaa tttttggtgg ttgttgctgc ttcctgtcat tttcgacatg tttatcacca
    41221 aatttattcc ctggacttgg tggaaaaaaa caaagaacaa aactcttcgt tctatttaca
    41281 gttggattga tgccattgta tttgctttgg tagcagtata tttcattaat atttacctgt
    41341 ttcagaatta tcagattcct acctcatcgc tcgaaaaaac tttgctggtt ggtgattttc
    41401 tgtttgtaag caaagccagt tatgggccgc gaattcccaa tacaccgctt tcttttccgt
    41461 tggtgcagaa tacttttccg attatcaaca caaaatccta tctggaaaaa ccccaatggg
    41521 aatataaacg cctgaaagga ttcgatacca ttgcacacaa cgatattgtt gttttcaatt
    41581 ttccaacagg cgacacggtt tgtctaaaac agcaaaaccc tgattattac caactttgct
    41641 actactacgg acgcgatgtg gttcgcagca gaaaagacat ttttggagaa atcgtgtatc
    41701 ggcctgtcga tcgcagagaa aattacgtta aacgctgcat tggcttgccg ggcgactggc
    41761 tgcaaataaa aagcaatcag gtttatatca acggagaaaa gcaaaaacca tttccgggcg
    41821 ttcagttcaa ttatttggta caaaccgatg gaacacgttt gagtgatgaa ttactcacca
    41881 aattgggaat aagcaaggat gaccgtttga ttttaaacac ttctcaaaac ccacaggaaa
    41941 taatggcttt gggtttggat ccaagtttcc ctatttatca ttttccgctg acgggtgaaa
    42001 tgctgcaaaa actgcaacga gttacgggtg ttgttaaaat tgtgattgaa ccggaaatgt
    42061 atggtggcga tgttttccca ttgggcggag gtaaaaactg gacccgcgac aattacggac
    42121 ccatttacat tcccaaaaaa ggagcagtac tggaattgaa tgcctacaat tatcccattt
    42181 atgaacgggt gattcgcacc tatgaaaaca acaagctgga atataaaaac ggaatttttt
    42241 atatcaatga tcgaccggag aaaacctatc gtttcaaaat ggattattac tggatgatgg
    42301 gcgacaatcg gcataattcg gctgattcgc gctattgggg atttgttcct gaagaccata
    42361 tcgtgggacg gccggtagtg gtttggttgt cattggataa ggacaaatcg ttgtttggtg
    42421 gaaaaatcag gtggaacaga tttttcaagg atgctcgaag ataaaacgga aaatatatta
    42481 tcgactgcaa aaattcgaga aattttctct tcggaattac acaactttat ctctgcaatt
    42541 cgagaggaga aagtgatgct ttctatgtct tatctggctc cgatagatta ttttatgact
    42601 ttgacaaatg cccgatcggc tgtgatcgaa caacatgaat attatcagaa acaaacctat
    42661 cgaaaccgat gcagaattgc cggagcaaat ggagtgttgg atctgattat tccggtggaa
    42721 aaaaccgcaa acaacaaagc tactgttcgc gatattcgga tttcgggaca caatgattgg
    42781 caacgtcaac attggaaagc aatttcttcc gcttaccttt ccagtccgtt ctttgaatat
    42841 tatttggatg atttccgccc attttatgaa aaaaattggt tatttttgtg ggacttcaac
    42901 atggaaattc agaacaaaat ttttgaattg ctggaaattt atcccaagtt ggaatttacg
    42961 gacaagttta ttgataaaat tgaagaaaat atactggatt tgcgtttgca attccatccc
    43021 aagaaacagc cctttctaaa aattccttca tactatcagg tgttcgaaaa taaattcgga
    43081 tttatttcgg gactgagtat cattgacttg ctgttcaaca tgggtaacga aagtctgtta
    43141 tggcttttgg gaaaagcaga ataaaaattt ttaatgagaa agaagaaact gattttgtgg
    43201 atttttcaaa aaattaaaac atataaatat ggaactggat tggaacaata ttggatttgg
    43261 ttatgtgaaa accgactaca acattcgttg cagttatcaa aatggtgaat ggggagaatt
    43321 tgaagttcac gaagatgaat acctgacatt gcacatggca gctacttgcc tgcattacgg
    43381 tcaggaagtt tttgaagggt tgaaagcttt tagaggcatt gacggaaaaa tcagggtttt
    43441 tcgcatggat gaaaatgcca aaaggatgca atcatcttgc aaaggaattg ttatgcaacc
    43501 tttccctgtc gatagatttc aggaagccgt tcttaaagtt gtcaaactga atgaacgatt
    43561 tgttcctcct tatgagtccg gagcttcgtt gtatatccgt ccgcttatga ttggaacaag
    43621 tgctcaagtt ggcgtcaaac cttcacgcga atatcttttt atattatttg taacccctgt
    43681 aggaccctat ttcaaaggag gctttcagac aactccttac gtcattttaa gagagttcga
    43741 tcgcgcagct ccgctgggaa caggaattta caaagtggga ggaaattatg cagcagggct
    43801 ggctgccgac gaaaaagctc attctttagg ttattcggca gttttctatc tggatgccaa
    43861 agagaaaaaa tatattgacg aatgcggcgc tgccaacttt ttcggaatta aaaacaatac
    43921 ttatgtaacg ccaaaatcga cttccgtgct gccttccatc accaataaaa gtttaatgac
    43981 attggccaga gacatgggca tgaaagtgga acagcgtccc gtacccattg aagagctcag
    44041 cacttttgaa gaagccggag catgtggtac ggcagccgtt atcagcccta ttcaacgaat
    44101 cgacgatttg gacgaaaata aatcctacgt tttttcgccg gatggtaaac cgggcgttat
    44161 ttgcgaaaaa ttatacaaaa aaattcgtgc cattcagtat ggcgaagaac ccgatatcta
    44221 tggctggaca accattgtcg aataaatttt ctatagaaga tggtcgtttc tttttctaaa
    44281 aaaattttgt ttgagaaatc aatttaaaca aatgtatcaa gcaaaaattt tttactgata
    44341 attttttaaa atcgaagcac agaaaaatgg ttcagttccc ggtaaaaaac atatttacgg
    44401 tttaatgaag ttatatcaga tcaaaaatta atacaagtta tggtggcttt tgaaaaatat
    44461 tttgttaaaa agaaaagaac attcgttttt ttgtcgatct tttttctttt tacaggtgcc
    44521 aaagccgacg aaggcatgtg gatgttgcct tacattgagc agctcaatat tcaaaaaatg
    44581 aacggaatgg gttgctcgtt gagcgcagaa gatatttaca gcgataaaaa tatcagcctt
    44641 aaagatgccg ttatcgtatt tggaaatggt tgtacaggag ttgtggtatc acctcaggga
    44701 ctgattttta ccaatcatca ctgcggtttt gatgccattc aacagcacag ttcggttgaa
    44761 cataattatt taaaagatgg atttaacgcc gtccgattgg aagatgaaat ctccacgccc
    44821 ggtcttagtg tgaaatttct ggttaaaatc gaagatgtaa ccaatcaagt tctttcccat
    44881 ttgccggata gtttgtccga aaaatcgaga aacgaaaaaa tttatgaaat ttccgacagt
    44941 atttgtaaag cagcggaaaa ggacacgcat tatctggcag aagtgaaacc tttctttgca
    45001 ggaaacgaat tttacctgct tgtttacgaa gaatttaaag atgttcgact tgcttttgca
    45061 ccgcccagtt ccattggaaa tttcggaggt gataccgata attggatgtg gccgcgtcat
    45121 acgggcgatt tttcggtttt ccgcgtttat gcgactcccg acgggaaacc ggccgaatat
    45181 tcaaaagaca atgttcccta ttctccgaaa cgttttgcgt ccgtttccaa caaaggttat
    45241 caaaccaatg attttaccat gattctgggt aatcccggct ctaccagccg ctatttgact
    45301 tcttttggcg tgaaattcag aatggaaacc ggcaatcagg cacgcattga tgtcagaggc
    45361 atcaagcaga ctatttggcg ttcgtttatg cggaaggatg aagcaatcaa tattgcttat
    45421 gccaataaat atgcttcgaa ttccaactat tggaaaaaca gcatcggcat gaataaagcc
    45481 attcagaaat taaatgttct ggagagaaaa agggaacagg aagctgcttt tttggaatgg
    45541 acaaaatcga atccggaaag agagaaaaaa tataaagatg tattgaaaac gctcgaagat
    45601 ggatattcta cgctgtatcc ttatgctcgt gcgctcaact atttgcgtga atcattgatg
    45661 agcggtgtgg aattgcccag aattgcccag aaagcaagtc aacttatttc gcaaaacttg
    45721 ccaaaagatt cgctgatcaa ggaaatagaa gaaatttaca aggattaccg tccggaagtt
    45781 gatcaagccg tttttgctgc catgttggat gtttataaaa actcggtaga tgcggatgca
    45841 cttcctgctt tttatcagga gattcaaaaa aaattcaagg gaaatacgac gctttacgca
    45901 gcttcaattt tccaaaattc cgcctttagt tcggtagata aattgaagaa aaatatacag
    45961 aaaaagaaaa ttaaaattgc caacgatccg gcattgattt ttggcaacga agtaagaaat
    46021 acgtatgaac aaatcagttc gggtatttat gctcaaacat ccgaaaaggt gaaagaagct
    46081 gaacgtattt atttttcagg acttagggaa atgtacgaag aacaagggaa aaaactttat
    46141 cccgatgcca actttaccat gcgaatgact tatgggaaaa tcggaggtta tcagcctgcc
    46201 gatgctgtgg attaccatta ttacaccaca accaaaggga ttttggaaaa agaaaatcca
    46261 accgatccgg aatttattgt tcccgataag ttgaaaaagt cgattctgga aaaagacttt
    46321 ggaaaatatc tggatcctca gacgaaagac atgcatgtaa attttattag caataacgat
    46381 attacgggag ggaattcggg cagtcccgtt ttcaatggga aaggagaatt aattggtttg
    46441 gcttttgacg gcaactggga agccatgagt ggtgatattg tttttgaacc ggagctgcaa
    46501 cgtaccatca gtgtggatat caggtatgtg ctttatgtaa tggacaaaat tggtggcgca
    46561 cagcgattaa tagatgaatt aagcatatcg ggatactaat ttttcagata agctcacttt
    46621 tttattctac gacaaatatt tacaatgatg acaatttatg gaaaaaactt cagaagaaaa
    46681 aaaaagtttt caatggaaag atgtttttca aaaaataata tctgatttaa aaacacttac
    46741 caatttacac gaagacacaa atattgaatc taccatagct tccataaaaa aagatgtaga
    46801 atttaagggg gctaatgtct ggattttatt ttttgctgtt atcatctgtt ccgtaggact
    46861 caatgttaat tctacagcag tgattatcgg tgccatgctc atttctccgc ttatgggacc
    46921 catcaatgga gtaggattgt caataggcat ttatgatacg gaactcctga ggttatcatt
    46981 gcgaaacttg cttataatga tattggtagg attggttgcc tctacgtttt attttcttat
    47041 tactcccatc actgatgccc aatcggaact tttagcgcga actcgcccta ctttatttga
    47101 tgtgtttatt gctttttttg gcggatgtgc cggcattgtg gctgcttctc gaaaagaagg
    47161 gaaaataact gttatttcag gtgtggccat tgctactgct cttatgcccc ctttatgcac
    47221 agccggctat gggatagctt cgggacagct acgttttttc ttcggagcat tttatcttct
    47281 ctttatcaac tcttttttta ttgctatagc tacttttttg gtaactcatt tacttcattt
    47341 tccagtaaaa caattcgtcg acatacaaag acaaaaagtt gtaaaacgtt ctattattat
    47401 attttccatt ttaacaatta ttcccagttt ttttctggca tggaatgttg ttagcgaaac
    47461 aagattcaat tcacatacct atcgttacat tcaggatatc caacaactcg atctgtttag
    47521 tgaaaaggaa atcatcaacg tttcgagaaa atattcaaga aaggaaaaat ctcttacact
    47581 ttctgtcata gggaaacctt tgaccgaaga gcaaatccga tacatacaac aattacttcc
    47641 caagtatggt ttgaataatg taaatcttat tatcaagcaa acagggggga cgttcgactt
    47701 aggtgctcaa actgcattta tcgaaaaatt actgatggac aaagatatcc aacttcaaaa
    47761 aaaagattca ataatagctg ctttaaacaa caaattgcaa ctgatgcaaa cttcaggggt
    47821 taatgagttg caacttgccc gtgaagtgaa ggtgcaattt cccgatataa aacaaatagc
    47881 cttttcaaat tcaattttta tcaatttgga tcatttaaca caagatactg ttcccactat
    47941 atttatccaa tgggaacaaa cacctcccaa taaaaaagca ttattgtccc agatgaatga
    48001 atggctgaaa gtgcgactta atttggaaaa attaaaaata atagaagttg aaatgtagtt
    48061 ttcaattttt ttacctggtt tcttgtaaaa aactttattc cccatgaaaa tagatgtttg
    48121 tttttcgccg gcattatacc ctttttattc gtcaggaaat tcaaaaattg cagtaatagt
    48181 ggatgttttc agggcgtcga ctaccatttg caaagctttg atggaaggag catccgccat
    48241 tattcccgtt gcgtcgctcg aaaaagccga agaattaaaa agtcgtggtt attttgtcgg
    48301 agcggagcgg aatgtggaaa agtgcgattt tgcagatgcc ggtaattctc cgttcgacta
    48361 ttattccgaa aaagtgaagg ggaaagaaat tgtatttact acaaccaacg gaacccgtgc
    48421 cattgaactg gcagcagccg aaaacgaagt aattatcggg gctttttgca acattagtgc
    48481 agtgtccgat ttttgccgca atgcccgaaa agatgtactt gttatttgtg ccggctggaa
    48541 taatcttttc agtttggaag atactttgtt tgcaggcttg ctcgtccata ggttgaagtg
    48601 cagcgggaat ttcgaaactt caggtgattc ggcggttgct gcattagttt tatggcaaca
    48661 ggccgagccc gatatcaaaa cgtttttgca tcaaagtgaa catgcaaaac ggttgtttac
    48721 gcatcatttg gaaaaagatt ttgatttttg tctggctcag gatacccttc cgatggttcc
    48781 gttttacgac aagtcttctc aaaaaatatt attttaacgt gaggttcggc ataagaatat
    48841 ttaataagca attataaata agctaataag gctgaaagga agtattccca tgttactaag
    48901 tctggtcgtt tctatataaa gtcattctat ccaaaaacga ggcttatttt taaataaaac
    48961 cttagtaacg ataattaata acaagaataa accttattaa ttatcaattt tttatatcaa
    49021 aacttcacta aaaattcgtt cgcctaataa aacgctaatc aatggtttaa ataaaaaagt
    49081 ttcacatgaa aaacatagat gaaggggtga ttagaagaac ttattttccc cagttgcgaa
    49141 gatatgtgta gtactgagaa caacaattct ggaaggactt ctcgaaggat tttaattggc
    49201 taaagtttaa cttgtctttg tttgaaagga aattaaccat agaaagcaaa ggcaatagtt
    49261 attccctttt caatatcatc ctgtaaaaca atgataaaca atgttcattt gattgtaaga
    49321 tttatatact tgtcctacgt acaaacgaac agaactttaa atgatgaatt tgctaaagaa
    49381 attattgact ttatgcaatt gcggcatgaa tcctgttttt aacgaacttt tgtgtaaaca
    49441 taccaatgta aatcgtgaat tttaaatttt atgggatgga aaatatttgt tgatatgaat
    49501 caataaaaaa cgaaaggcat ctcattgaac agagacacct tccgttaaaa atacagttta
    49561 ttctttcaga ttattaaacg ccgattactt caaaggaaat ttcgacgctc acttctctgt
    49621 gaagtttaag cgtagcttta taggaaccta attctttcac cggttcgtcc acaacgatta
    49681 ctttgcgatc aataactaag cctgcttttt cataagcatc ggcaatttga atcggaccta
    49741 cggcaccaaa aatttttcct gttgaactcg ctttggcttc aataaccaaa ttaatacttt
    49801 tagccttttc tgccaattca agcgcttcgt ttttaatgcg ttctagttta tgagcacgtt
    49861 gtttcttttc ttcttcgagc attttctttg ccgaatcggt tgcaataacg gctttttttg
    49921 tggggataag ataattacgt gcgtaaccat ctttcacttt tacgatatct cctttataac
    49981 caagattatt tacatcttct tttaaaatta cttccatttt ttgtcctcct gattaaatta
    50041 tttcaacatg tctgttacat agggaagcaa cgctaaatga cgggctcttt ttatggcctg
    50101 cgcaactcta cgttgatact ttaaagatgt gccggtgatg cggcgaggaa gaatttttcc
    50161 ctgctcgttt acaaactttt tcaaaaattc cggatccttg taatcgatat atttaatacc
    50221 actctttttg aatcggcaat attttttctt tttgatatca accgtaggag gagttaaata
    50281 tcttatttct tcgttgttgt tggttgccat agtttattcc tttccttctt tagattttac
    50341 attttttctt ttttctgcat actcgtaggc atatttgtcc aaacgagtcg ttaaataacg
    50401 aatcacccgt tcatcacggc gataattggt ttccaaaact gccactacgg acggttcggc
    50461 gtcaaactgc agcaaacaat aaaaacctga ggattttttt tcgattggat aggccagctt
    50521 gcgtaagccc caattttctt cattggtaat tgttgcacca ttttcctgca aaatggtttt
    50581 aaatttttct accgcttcct tcatctgcag gtcagataaa acgggagtta aaatgaaaac
    50641 ggtttcataa tgattcaaca tactgtttaa tagcttttta tgtttataaa ttttatttgt
    50701 taaagcggtt acaaaggtaa tgaatatttt tgattttcaa aagaaatgtt tttattaaaa
    50761 tgttgaatac atagggttga gatttttaga aacaattttt ttcccttatt ccgaattttt
    50821 tttgacaaac gaaagaattc tattattttt gtcttaaatc atccccgctg taaatagaaa
    50881 cagctgaatt gaatcttttt tttaattttt ctaaaagcag tcaaatcaga gaaaatacga
    50941 caatggcata agaatttgtt aacaacaaaa aaataggagg aaaatttatt atgagtaaag
    51001 ttctttatat caaagcgaat gctaaacctg agggcgaatc cagaacattc atgattgccg
    51061 ataaatttgt ggaagcatac aaaaaacacc atcctcagga cgaaattatt acgctggatt
    51121 tgtacaagga agacattcat tttttaacag ttgaggatgt catgattcac atgagcaatg
    51181 aagtggaaga aacagaaaat gcaatgaaaa aatacgctct gcaatttaaa agtgccgaca
    51241 agtacatttt tgctgaacct atgtggaatt tgggggtccc ggctatatta aaagcttata
    51301 ttgattatgt aagtattccg aatatcactt ttaaatatgg cgagaatggc ccaattggtt
    51361 tgtgtcaagg caagaaagcg cttaatgtta ttaccagcgg cggcgattat tccgaaggaa
    51421 tggctgcaga atgggatttg tgcgataaat atctcaaaac cattatggga ctttttggtg
    51481 tattggatta tacaaccatc agagcttcta accttgatgt gatcgggatg gatgttgatg
    51541 caatagtcaa taaggccata aaagaggccg aggagttggc aaaaactttt taaaaatcaa
    51601 aagaataaat tttttaatcg aaggggaaaa agaaaaaaga gaaaactctt tgttgattca
    51661 gaaaaaaatc gtatctttgc aaactcaaaa gaaaatacaa acatttaaga cagatatgaa
    51721 aagcggaatt catcctacca attatcgtcc ggtagtattt aaagacttat cgaatggcga
    51781 cacatttatc actcggtcga ccattaatac taaggaaacc atagaaattg atggcgttac
    51841 ttatccattg gttaaattgg aaatttccag ttcttcgcac ccttatttta cgggtaaagc
    51901 caaattagtt gacagtgccg gtcgtgttga taaattcatg aatcgctacg ctaaaagttt
    51961 ggagaagaaa aaaactcaaa agtaattttt ttcataataa tgttgtgtaa aaaggcttca
    52021 gaaaaatttt ttgaggcctt ttttattttt cccttatcca ttttaaatcg ctccattgaa
    52081 acagcaaagg tgccgtattc atgcagggaa aaacgagttt tttatcttcg tcgctcaaaa
    52141 aatcgatgct gttgttgaaa gccattacaa tttgtccttc ctcctgaaaa ctgagcgaaa
    52201 caaaagtata tttcaagatt ttttgaaacg tttcaacccc ttcttcagtt ttttgggctt
    52261 tttccaaatc gagccaatct gtggcctgag gataagggaa agttacagga aggcgattcc
    52321 atttaaaatc atagaattca atcaaagaat gacatattgg tgcacaaact gtgcggataa
    52381 tggcaatcat cggttcgttt gagttggcat ccaaaacctt catttcaaaa gatgttgaac
    52441 ccgaattttt caccattaag tgttgatgaa gcgtatcgag ttttaacaga aaaaccggtt
    52501 ttttatagcg attgagggtg gtatcgtttt gtccggcttt ggcgtattcg ataagttcga
    52561 gccgttgttg cgacgtcaaa gtcggatgaa gagcatccgg catgccgaca tacaacttat
    52621 cgatgccttg tgccgaaatt tttgcacaca aaaagaaaaa aatcaccgta aaaaaattat
    52681 tgatcagctt cattgttttt ctttttaaaa gtgagaatgt tccaataata acggaacaat
    52741 tcgtcaaaat ttgaaaaatt ttctttatat atcaatccta ttccttgcgt attctttgcc
    52801 gaccgaagtt gatagcggtc aatggtgtgg ttgtaggctt ttaatctgaa tttaccacct
    52861 tccgtgagta accattcaac gtcaatgtcg ccaataaatt tattggtatt gttgctcaaa
    52921 tttacattat catttcgata acccaaattt ccgttgataa ttagccggtt atttggctga
    52981 ataagaaatt gagcctgaac atcactgctc aactgatctg tttgacggta atcgaatcca
    53041 atggaaataa tgttgctgtt gatcatttga gccagccaat tattcatttg tgcacttaaa
    53101 gtactgacaa ccaatgatac ggcttcgctg gaaccaattc caacgggagc gttagcagcc
    53161 gactgcagat aagggttgta aaatttgttg aaaatgagca aataaataat ttgtcgcgcc
    53221 atcatttcgt tggtgctgat gatgcttttt accatctgtt ttacactttc gtcgctcgag
    53281 ggcaaatcaa tatcaaattc tacattgggt ttcatcaaat tcccacttaa caacaatata
    53341 caatttaccg gcacagtggt tcgaggcaac gaactcaatt gagattcatc cattaaatcc
    53401 cgcagagaag ccatcaaaga ataaattccc tttattttta cctgcgcatt ataaagatcg
    53461 ccggtccaag tcagcgtact tccttcatca attttaaatt cttttcgaat cacattttga
    53521 agtgtaaaaa gatagtttcc tttttcgatg gtgtaagtgc caaagagttt tatgtcggaa
    53581 taggtatcaa attcaaagcg cagatttcct gttccttttc caacaagcat atcgccggct
    53641 ttcggatcga taataaattg aatttcagca tcgggcgtaa tgcctatctg aagattaacc
    53701 tttgtattgt atgtattacc tggcagtttt ttcccttttt gctcgtccgg ctgggtcttt
    53761 attgtgtcat ttttgttgac aaagagaaca aaactgttgt cggcagcagt tgaagagccg
    53821 ggcatagtta catagcattt ggaacctggc cgtgaaacca tgtccaccca aacattggta
    53881 acattcacat caccaaaaac atgaaccgta cctcctgcat aagcttttcc gtagaatatt
    53941 tcattggcgt tggcatgggt attcaatgcc aaaatattat tggagcgaat cattacgtca
    54001 tacttcaggt cgccaaacat tcctgtatgt ttcattattc catcaagtaa gccttcattt
    54061 ccttcttcat catacatctt tatattattg aactcgacag aagtaggcct catacgaatg
    54121 gtatcgttaa aaaaataggt tgtttgcgtt agtgccattt ttaattgtcc ttcggccaca
    54181 tacaaatctc catcaaaccg catttttttt gtcggtccaa acatccgaag tttgcccgtt
    54241 gcaaatcctc taacattttg aaccacatca ccaaaatatc gtttcaacat tcccatattg
    54301 aggtgatgca tgtcatacac gaaatcaatg gaatcttttc ccggcacata aacgccgtca
    54361 atgtaggcta ccgtatcctt gttgtttaag acatgagcat ttgaaaccac ttcttttttt
    54421 tctttgtccc aataggaata caaattcaca tctcccaaaa ccgattggtt catggaaaaa
    54481 tcctttacca ataatttagc ttcgaaaaaa ggttgctgaa atgcgctgta catggttgca
    54541 ttgcccgtaa aatatcctcc cagcgacgga tccttgagtt ttaccaaatc gttcatgata
    54601 aaatacaaat ccaaattgtt catggtaaac tgcaaactgt ctttttcact ttttgaagca
    54661 atgccgttaa catgaaggaa ttgactgctg ccctccattt tgaaattatt tacttgaagc
    54721 gaaccatcag aattgtattt tacaacagat gggaaaatat ttaaaacgga atccataaga
    54781 ataatctgac ttggaagaac agagatttgt gcattgattt ttccatcggt tgctgcaaat
    54841 tttgtcagtg ttttaaattc tcctgcattg gtaattgaat cggaattttg ccagcccaac
    54901 aaagtattga cagaattgtc cgatgctgtt gcattgatat aaaagtgcat gggcttgttt
    54961 tttccatcca tttgtgcgcg ggtaataaat gataatttat cttcctcatt ctgaagtgat
    55021 aaaaatatgt tttcaaattc cctttttcca aatttgaaat aaggaaaatt tgaagtgaag
    55081 tcaattttat aatttttttc gtcataatat cctttaatat atgaattgcc tttcaattgc
    55141 catggaagtt ccaaaatatc ggtcagttca cctgtatcag aaattttcaa atcaacattg
    55201 ataaaatttg gatgtgaatt ttcgtgtgga attttgttcg acaaggcagg taaataatga
    55261 tatgccaatc ttttaaaagt ttgatctaat gagctgtaaa taaaatttcc cgaaatactt
    55321 ccattcacgt agtcggaaga aattaaaaaa taggtttcat cgttcttcgt gcgcgaaacg
    55381 aattgaattt tgccggcatt gagtgttcga tttcggttgg tgaaagctat cttgtcgaat
    55441 aaaacgaatc cgttgagatt gtccggtgaa tttcctatca tattggttct tgcattgaag
    55501 gacaaattgg cattggaata cttagttgtc agtttcaacg cattcaaatt ggctttggcc
    55561 aaatttaggc tgaaatcgaa tacaggtaaa cgttttgtca tatcgacaat tccggtaaaa
    55621 tgagcatcga tattttcgtc ggccacgtca aatgtgccat caaatccact tccatcgtaa
    55681 tttcctttta atttcatatc gcggtaggaa taaccattcc attgaaattc gggaatatca
    55741 gcttcaatag ttccctgaaa atttttattg gcctgttttt ctcctttggt gttaaaatta
    55801 aatgaaaaaa gtccaaactg attgttgctc agaattttat tcaattgaat gttgtccgac
    55861 ttaatggtgc cattgtaagc caaatactga gacttggtat tgtattgcag caggatatcc
    55921 gtagctatcg tacccacatt cgatctcagt ttaccatagg ctaccaggtt gtttagaaat
    55981 ccggaaatat tgccgttgta tctgaccatt cccagttgat tcaactcttt tggcagcaca
    56041 aatggttttt ttgttagtcc ggcaacaaaa tcctgcaaat cgtttttttc gaaatgcaaa
    56101 tcattgatgt tggcgtaaat aaaagtttca tccatattgg gtaaaccggt aaaatcggct
    56161 ttggcattca gtaaaaagga attcccaaat tttaatcgaa tttggttgaa tttcagactt
    56221 gaaattctgc catcgatttc aatttctgca tccattttgg tatttaagtt ggcaaaggca
    56281 gggacaaatg cagcgaaatc agaaaaggta aagtaagaag atcgaagtgg aattttcaat
    56341 ttcacattct cattcagatt tttcaaatcg gaaaatgtag cataatcgac gcgaatactg
    56401 tcaaaatgaa ttttagactt gggcatccat aaatcgaaat agggaatatc gatgttttta
    56461 tttgaaactg agaattttgt ccgtaagtcc tttaatgtca agcctgaagc ttcgtgtgcg
    56521 ctcaagtggt tgatgcttgc cttaatggta tctttgtgaa aaatatccaa atgaatatcg
    56581 gaattaatat tggtaagata aagatgcttg ggattaaatg tctgcgagtt gccctgattt
    56641 tggcgtgcat cgacgaaacg aaaggaagaa ttttttaatt tgaatcgggt aatttgaaat
    56701 tctatatttg ccggcttaga accttgtggt tttttgaaag cctgaacaat aaagtcaaaa
    56761 tttgttttgc cagccttatc aatagcaata ttcccataaa gttgatctaa atcaatggaa
    56821 cgaaattgat attttccacg aaaaaatttc catagattaa aatgtgcatc catgcttttt
    56881 acatacaaaa gtgtgtcttg ttgctgatcc tccaagtaaa cgtcatcaat ggcaatggta
    56941 ttgaaaagct tgtaatgtat cttaccgatt tcaactttag tttgcaattt ttctgaaagt
    57001 tgtcgaacga gggtttgcgc aatagaattt tgtattctgc tgttttgcaa caaaataagt
    57061 aaaattgcaa ggaataaaat aattcctcca attgtataac ctgctatttt aactgatttt
    57121 tttatacctt tgaattttat tttacaaaaa taaaaaaatt atttatgatt ttctgaatca
    57181 aaaaagtaga acaaccctta tttttttata aaattcaagc gtactatttc tgattgagaa
    57241 tgttgttttt tattaaaaaa tgaattttta tgaaccccat tattatatta ggaatcgaat
    57301 cttcctgtga cgacacttcg gcagccgttt tgcgcgatgg agtcatgctt tcaaatgtta
    57361 ttgccaatca gtcggttcac gccaaatttg gaggagtagt tccggaattg gcatctcgtg
    57421 cgcatcagca aaatattatt cctaccgttc aggaagcatt aagacaagct gacatcgaca
    57481 aaaaacaatt aagtgctgta gcttttacgc gaggaccggg tttattaggc tcactgcttg
    57541 tgggaacttc ttttgcaaaa ggttttgcac aggcactcag cattcccatt attgacgtga
    57601 atcatttgca ggcacacgtt ttggctcatt ttatcagaaa agatgaaaac gacaactcac
    57661 cgcaacctca attccctttt ttgtgcctgc ttgtttcggg aggaaattct caaattgttc
    57721 tggtaaaagc ttataacgac atgaccgtta tcggccagac cattgatgac gccgcaggcg
    57781 aagcttttga caaatgtgca aaaattatgg gtttgcctta tcccggtggc cctcatatcg
    57841 accgtctggc aaaagaagga aatccaaatg ctttcagact gaataaaccg caaattgaag
    57901 gctacagcta cagttttagt ggtttaaaaa cttctttttt gtacttgctt cgggacaaaa
    57961 tgaaggaaaa ttcaaatttt attgaggaaa atctcaatga tttgtgtgct tctctgcagg
    58021 caaccgttgt cgatattttg atgaataaac tgaaaaaggc ggcaaaggat tacaacattc
    58081 atcaaattgc actggctgga ggtgtttcag ccaattcagc cttgcgtcag gcttttaacg
    58141 atttggcaac ttttgatggt tatgaagttt ttattcctcc ttttgctttt actaccgaca
    58201 atgctgccat gattgccatg accggttatt tcaagtatct cgatcacgat ttcagttcct
    58261 ttgacgatgt accgtttgca agagttacca atacttttta agaatatcat gaaaaaggag
    58321 ctttccagaa taaaaaaaaa tttgactcct tacaacggtg taatttattt tgttgttatt
    58381 ttgctaattt gtcattttgt atggaaatta ctggtacggg gtgatgattc aaaccgtcag
    58441 gttacgtttt tggggaatga tatttcggcg ccatttcaat ttatggccaa ccatgttgct
    58501 caaactactg caagcgtttt aaatttttta ggatttcata ttcagctttt tgccaataac
    58561 gttctgaaac aggaaaatgg cttcggtatc caaattgtat ggtcgtgtac cggcctgaaa
    58621 caggcttata tttttttctg tatcatagct ttttattgtg ggccgatgag aaaaaaattg
    58681 tggttcatcc cgctcggact ggctgtggtt tatgtgttta atattttcag aatcgccgca
    58741 ataacggctc ttgttcagca acactcttct tggtttactt ttttgcacga atattttttc
    58801 aaatacttat tttatgctgt aatttttgct ttatgggttt tgtgggaaga aaaatttgta
    58861 actaaaaaag aaaagaatga aacccacatg acacaaactt gacacccgag taatgaacgc
    58921 cgttaaagcc acagatatct cttcccaagt agaagaa
//
LOCUS       DAOSCH010000004        43630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_660_length_43630_cov_5.831830, whole genome shotgun sequence.
ACCESSION   DAOSCH010000004 DAOSCH010000000
VERSION     DAOSCH010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 43630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43630
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_660_length_43630_cov_5.831830"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(179..2989)
                     /gene="uvrA"
                     /locus_tag="PLZ54_00790"
     CDS             complement(179..2989)
                     /gene="uvrA"
                     /locus_tag="PLZ54_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOJ65510.1"
                     /translation="MSKQGNIIVKGARVHNLKNIDVEIPRNKLVVVTGLSGSGKSSLA
                     FDTIYAEGQRRYVESLSAYARQFLGRMSKPEVDYIKGIPPAIAIEQKVNTRNPRSTVG
                     TSTEIYEYMKLLYARIGKTYSPISGELVKIHEAEDVVEAAMSYPDDTKLTLLIPIRLN
                     EENNLTEKLRNFQMQGFSRVEAGNEMLRIQDLLLRPELLEKNTEIYLVIDRLVVDHSP
                     STISRLTDSVETAFYEGNGNCILKVNTEEGEKLLHFSKNFEADGITFEIPTEHTFSFN
                     SPIGACPVCEGFGKIIGIDEDLVVPNKSLSVYEDAIVCWRGEVMSEWKKRLIQAAPKF
                     GFPIHKPYYELTESQRQLLWTGNEYFEGLNSFFKFLEENQYKIQYRVMLARYRGKTTC
                     PACKGTRLKKEATYVRVGGKPITELVLMPVEELYDFFEQLSLDGHDAAIASRLLTEIQ
                     NRIRFLLDVGLGYLTLNRLSNTLSGGESQRINLATSLGSSLVGSLYILDEPSIGLHPR
                     DTEKLINVLHQLRNLGNTVIVVEHDEEIIRSADYLIDIGPGAGRLGGNIVFNGQLNDI
                     DKQENTKETIANSYTLKYLYHTEEIPVPTLRRKWNNYIEIVGATENNLKNISVKFPLN
                     VMTVVTGVSGSGKSSLVQDVFYAALKKYYGGVVDHTGQFGVLQGSLQLISQVEFVDQN
                     PIGRSSRSNPVSYIKVYDDIRKLFSEQPLAKQMGYNPSFFSFNTEGGRCEECQGEGTI
                     TIEMQFMADLVLECEHCHGKRFKQDILEVTYRGMNIYDVLEMTVNQAIEFFGQSNNTL
                     EKKIVKRLKSLQDVGIGYVKLGQSSNTLSGGESQRVKLASFLANEKSDPTLFIFDEPT
                     TGLHFHDIKTLLKAFDALIAKGHTVLIIEHNPEVIKCADHIIDLGPEGGDKGGYVVFE
                     GTPEAIVNCGNSYTGKFLKGKI"
     gene            3341..4483
                     /gene="dnaN"
                     /locus_tag="PLZ54_00795"
     CDS             3341..4483
                     /gene="dnaN"
                     /locus_tag="PLZ54_00795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOJ65511.1"
                     /translation="MKFIVSSTELLSHLQTISRVINTKNTLPILDDFKFSLEGNTLTM
                     TASDVETTLITSIQVEESEGEGQVAIASRLLLDTLREFSEQPLTFSINESNLSLTITT
                     SNGAYNFIGENADEFPRLPPFEEEPCSFELPVDNLLSGISKTLFAAAADDLREVMNGI
                     YFDLSPKELTIVATDAHKLSRYKTTNVSTSLDEDEVKSFVLPKKPANLLKNILPREKG
                     NVKIRFDKKYIHFELENYTMVCLQVAGKYPNYNSVIPFNNPIKIIVDRLMLLNSVRRV
                     SVFCNQATNLIKLVFNEQQVNISAEDIDFSISAEETIPCQLEGNAITIGFKATYLIDI
                     LSNIDSSDVVIELSDPSRAALISPLENEENEEVLMLLMPMLLMDYD"
     gene            4591..5361
                     /locus_tag="PLZ54_00800"
     CDS             4591..5361
                     /locus_tag="PLZ54_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOJ65512.1"
                     /translation="MELNLKKPIVFFDLETTGINIVTDRIVEISYHKVFPNGKELTKT
                     KRINPGIPIPAYATAVHGITDADVANCPTFKEVAKEIAADFEGSDLAGYNSNRFDIPL
                     LAEEFLRAEVDIDLMKHRFIDVQVIFHKMEQRTLSAAYKFYCNKDLNDAHSAEADAMA
                     TYEVLKAQLEHYPELKNDVDFLSDFTTQNNNVDFAGRVIYNEKGEEIINFGKYKGKKV
                     EDVLKTDMGYYGWIMNSDFSLHTKKVFTNIKLRQFNNK"
     gene            5597..6421
                     /locus_tag="PLZ54_00805"
     CDS             5597..6421
                     /locus_tag="PLZ54_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOJ65513.1"
                     /translation="MNKIVNKEYLSPNVVKFEVEAPLIAKARKAGHFVIVRVGEKGER
                     IPLTIADANTEKGTITLIVQKIGLSTSKLCELNEGDCITDLVGPLGKATHIDNFGTVV
                     CACGGVGTAPMLPIAEAMKKAGNHVITVLAARTKELIILEEQMRKFSDEVIIMTDDGS
                     YGNKGLVTDGVEMVIKRQKVDLCVTIGPAIMMKFVSELTKRYNIPTIASLNTIMVDGT
                     GMCGACRISVGGKTKFVCVDGPEFDAHQVDFDEMLIRLNGYKDIEREQLHKISPNN"
     gene            6489..7955
                     /gene="gltA"
                     /locus_tag="PLZ54_00810"
     CDS             6489..7955
                     /gene="gltA"
                     /locus_tag="PLZ54_00810"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOJ65514.1"
                     /translation="MTNFNEERMQPWRVELRNSMKPKDRMAIARVKMPELDPEYRSHT
                     MKEEVNLGLTPEMALMESKRCIDCANPTCVQGCPVAINIPKFIKNIERGEFLEAAKTL
                     KETSVLPAVCGRVCPQEKQCEAQCIHLKTKNEAVAIGYLERFAADYERETGNISIPEI
                     AAPLHKKVAAVGSGPAGLAFAGDMAKMGYEVVLFEALHEIGGVLRYGIPEFRLPNKIV
                     DAEIRNLIAMGVHFVTNCIIGKTITIDDLLAEDYKGIFIGSGAGLPRFMNIKGENLVG
                     IMSSNEYLTRVNLMDAANPESDTPVIKGKKVAVIGGGNTAMDAVRTARRLGAEKAMIV
                     YRRTEQEMPARVEEIKHAKEEGIEFMTLRNPIEYYGDKNGRVTHMLLQVMELGEPDAS
                     GRRSPVEVKGKTELVEVDEVIVSVGVSPNPLIPQSVKGLEVTGRGTLVVNEETMQTSI
                     PYIFAGGDIVRGGATVILAMGDGRKAAKGMNEWIRSFT"
     gene            8048..8236
                     /locus_tag="PLZ54_00815"
     CDS             8048..8236
                     /locus_tag="PLZ54_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HOJ65515.1"
                     /translation="MENEVSVKVAKGGPYIVSGNFKLVLPSGEEKEMSGKTALCRCGQ
                     SKNKPFCDGSHHDTEFDK"
     gene            8398..9204
                     /locus_tag="PLZ54_00820"
     CDS             8398..9204
                     /locus_tag="PLZ54_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ65516.1"
                     /translation="MDKLRFQSFGSGSSGNCYYIGNAEYGFLIDAGMGSRTIQNCLKN
                     IGLNFENIQGVFITHDHVDHIRSLWALERKFHLPVFSTRMISEAINQYYYREKNINLP
                     KRRYIEKNETLDIRDFSFTAFDISHDATDNVGYSIEYKGKRITFATDLGCIGKTAASY
                     LILSDYLVIEANYDEQMLRNGKYPDYLKKRISSSRGHLSNDQTGYFLSENYTEKMKNI
                     FLCHLSKENNSPQIALKTIRDYMENKNISVGKDVDVVALNRLNASELYEF"
     gene            9230..9979
                     /locus_tag="PLZ54_00825"
     CDS             9230..9979
                     /locus_tag="PLZ54_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007663979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOJ65517.1"
                     /translation="MGENLVIIPTYNEKENIENIIYAVFRQPHDFHLLIVDDASPDGT
                     AEIVKNLQQKFPDRLFMIQRPGKMGLGTAYITGFKWALQHDYQYIYEMDADFSHNPDD
                     LPRLYASCALDGADMAIGSRYLTGVNVLNWPLKRVLMSYFASYYVRIVTGLKVTDTTA
                     GFVCYKRKVLETIDLDKIRFKGYGFQIEMKFTAEKCGFQLKEIPIVFINREYGTSKMS
                     SGIFGEAFFGVIQLKIESWFRDFPKKAIKSS"
     gene            9990..11345
                     /locus_tag="PLZ54_00830"
     CDS             9990..11345
                     /locus_tag="PLZ54_00830"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOJ65518.1"
                     /translation="MSELQLIRNAQIVNEGKIFIGSVLINGELIESVHEGKNNYGEID
                     PNKVEIIEGDNLLLLPGVIDDQVHFRDPGLTHKGDIYSESKAAVAGGVTSFMDMPNTR
                     PPAVNNELLEEKFQLAAEKSLANFSFYIGATNDNLDELLKINPQQVCGIKVFMGSSTG
                     NMLVDDEKTLNAIFRDAPCLIATHCEDELTIRRNKEYYQSLYGENLPAHFHSLIRNED
                     ACFASSAKAVSLAKKYNSRLHILHLSTEKEMSLFDNSVSLEDKKITAEVCVHYLWFSN
                     KDYEQLGNKIKCNPSIKSETDKLSLIRAINNNTIDVIATDHAPHLLPEKEGNCLNAAS
                     GLPLVQHSLVTMLQLAKQGYFTIEKVVEKMCHAPARLFKIEKRGFIRPGYYADITLVH
                     PNDTWQVSKQNIFYKCGWSPFEEMSFDFRVLKTWVNGNKVFDEGKFDEQVRGKRLFFR
                     V"
     gene            11419..12546
                     /locus_tag="PLZ54_00835"
     CDS             11419..12546
                     /locus_tag="PLZ54_00835"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOJ65519.1"
                     /translation="MEFRDLKKQYKVLKNDIDQAMLEVSASGAFIMGKPVKEIEQQLA
                     EYVGVKHCVSCANGTDALTLALKTWGIKAGDAVFVPDFTFFASAEVVSLEGATPVFVD
                     VDADTFNISPASLERSIEKTIQQGKLRPKVIITVDLFGLPADYSAIRKIADKYQLLIL
                     EDGAQGFGGEINGKKACSFGDISTTSFFPAKPLGCYGDGGAVFTDNDEWASLMDSLRV
                     HGKGSFKYDNVRIGMNSRLDTLQAAILKVKFDAFRKYELNDINKVADFYTEKLKDLVK
                     TPFIPKGYLSSWAQYTIQLQDEKQRDGLQAYLKEQGIPTMVYYPKPMHEQTAYLDLGN
                     LSGDFSVTESLCRRVLSLPMHPYLSMEEINKVCQTILDYLK"
     gene            complement(12592..15231)
                     /locus_tag="PLZ54_00840"
     CDS             complement(12592..15231)
                     /locus_tag="PLZ54_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ65520.1"
                     /translation="MMGIRIEKISAENLGPLQRFNYEMKNVNLIYGKNEDGKSFLTEF
                     IIRSLFSGTRQWGELREAGNGRIIVSGFNGQEKTFSPRTKKNEKLDVLFSDENGLSPA
                     LAKLLIVKSGEINIAENNLNGIDLNTLKELLSSQKTLNTLAAKISPTIKNSKIDGNLI
                     TINRQGEGKIYYDELSNLNKIRHFIELVSSQSETGKIQELKEEFRLLKKKNEKLLLAR
                     RYKAWQYATEIENVHHQLENISESELSELETKIKNCRELQSQKNQLSIEIKTLEERTK
                     DFDETKNNFDRQIAAKQYAAYALNQKISALEKQIDAVDEKEISELEKNLELHNLYQTE
                     ISDKEPKLEELINQTQHFDWLEKVRTAYQQLQFSSSEKDTLNKILMVLSVVFAAGGLG
                     TLLALKNILFGAILFAGSFIALSFYVFRLQKTFKNKLRNSEWNQIKQEFQEKFHQELN
                     QVNLDTLYDALNKKIVELGYMQEDRKKTEQKILELRATIKEKTLGIFGQEVEMSKIYE
                     RLSSLKKQRNELNRHKEQLKQELMMLQVDVSDYSPNDPCVAYSKNLLDSLSKKMTELN
                     IWREQKGEKETRLRQLSELTTALKNEILESFRAIFGTEIPENKWEQNITERRQFQTQL
                     KEKLSGLTGELKGLGVSPQDYVQENQEIEFSAEEQTKLQNEMETLKEKISELENGIDK
                     LKSEICTCLNIDFTTGWNEVVENLYEREKETLSNLKKNEAYIVAANVLYKTIKQLQQQ
                     EDEELAAGINSGIFTNLLYRLTGKYNKLEIADNEIIISSENNSWLLKELSTGTKEQVM
                     LALRITLAQRALNETAFLMLDDAFQHSDYERRPKIIDQLFELSSAGWQIIYLTMDEHI
                     RDLFNQTAQHKQFLENYQEICLS"
     gene            complement(15241..16299)
                     /locus_tag="PLZ54_00845"
     CDS             complement(15241..16299)
                     /locus_tag="PLZ54_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ65521.1"
                     /translation="MKIAITADVHLRTKAETPERYMALETLVNSMRDFDVSTLVVAGD
                     LFDKDFHNYADFDAFCKLHSSKHFLVIPGNHDLLLEQKYFTASNLEIVSSPEIKILGS
                     IPFVFLPYDAKLSMDEALSNFFIQRKGEEELKNWVLIGHGDYVSRSREMNPYEKGIYM
                     PLSSGSVSAFRPSKVVLGHIHKPYTSGSVWYAGSPFPLDVNETGKRRWLLFDTDDNSL
                     VSVGLPASVIYFVENIVVLPSDNEISYLTQQLEQMIDNWQLLPEDFSKVKLRLSVKGF
                     TTDKAKISGFVNDFLHRKGIGFYDETGIDTTELNILKDEDRISLFEKVKKIIDELPNF
                     DYAGKDDIMEQTLKLIFV"
     gene            16584..17954
                     /gene="radA"
                     /locus_tag="PLZ54_00850"
     CDS             16584..17954
                     /gene="radA"
                     /locus_tag="PLZ54_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOJ65522.1"
                     /translation="MAKTKTMYVCQNCGADSPKWIGRCPSCGEWNTYVEEVVSKGNAE
                     KIISGGFTVTAQKPVLLSEVEAIEEKRINTFNTELNRVLGGGLVPGSLVLIGGEPGIG
                     KSTLVLQVVLHLKGKRTLYISGEESVKQIKLRAERLKFDDPDCYLLSETSLEQIFVHI
                     QNIQPDLVIVDSIQTVSTEMLDASPGSVSQVRECAAAFLKFCKTTSIPVILIGHINKE
                     GGLAGPKVLEHIVDTVLQFEGDRHYMYRILRSIKNRFGSTSELGIYEMRQDGLREVEN
                     PSEMLFSQDHEGLSGIAIASAIEGIRPFLIETQALVSSAAYGMPQRSSTGFDLRRLNM
                     LLAVLEKRVGFKLNQKDVFLNIAGGLRVNDPAIDLAVISAILSSNVDIAIEKQVCTAG
                     EVGLSGEIRPIHRIEQRILEAEKLGFKKMIVPANNLKGLSLKNIEMEIIPVKKVEEAF
                     KILLKK"
     assembly_gap    17961..18060
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18070..21669)
                     /locus_tag="PLZ54_00855"
     CDS             complement(18070..21669)
                     /locus_tag="PLZ54_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ65523.1"
                     /translation="MRQSGKISFFGFLLVFVLAGCFCEYFYQTSPQRVVHFNQFQSKL
                     ISKELSTARILKQMSNLLDNHSVNELINFSFPDDDISFYVYRGNELIFWSDHHLDVSS
                     ISLNDSVELHFVQLPNAYCVSKTLSADSLKLLALIKIKNNFPYENDQLINDFAKGFNL
                     NKNVGIVGGSNLDKYAVFSSHGDYLFSLSEPTVPVYDERWALAGLISFLLFFLLFFVW
                     YARGFSLSRTKSLQWKDFCGYVLITGALLAVMLYFNFPSVLLANKLFAPYLYAVPPLL
                     NSTVHLTLLTVYIFSTVFLFYRHVQLGNSVSFLRSFGLLLMLPLYFLLFYYVLRGVVY
                     HSGPQILILSFKNFSIFTVWMHILMLLWGIGFALLFFKIHTFLTRHYYMKIAFAIDAI
                     FSCLLAMAAFKVFPEMWKLSFFSYLFLWIPLYGFYIVNFSRKRHVKLIIYVLFFSIFF
                     IGNVVVLSNEKKFNKYRIAAENLSLNGTIENDQMTEILMEELDAQLSRDRFVNQLMRN
                     SDSVEVLSEYLNNSYLRGFWNRFEVHINAVSKNSDLCKEYQLFLQEKGIKIKQTHFYN
                     IIPNEKNISYLGVFSVLTAGNDSTCYFMEFYPRQNFRSYSFPNLLISSPPSILSQLNV
                     SLARYDHRKLVYRNGNFVYPDVSNWIPFENSNYFVVHYADRRHYVYAPDRNTYLVLTE
                     NVQYGEGAYLLYFCYAFLAFFALCWLLQQVDDFFAKGRKFTLSFINRFQISFIALLIL
                     SFIGIFYSSVDYIRNNYEKEQIQNLENKKIYIQKALQNLYYWNKDLSDKNTQTLNLDL
                     QDLSYTFHTDVHVYNNDGILVGSSQPLIFSKKLISNRIAPKPYFTEDANINQYENIGH
                     LNYLTAYTDFFNGDYLQIGYIAVPQFFSQDEIQNEIESFISVIVHIYFVIILLVIILT
                     LLIGNQISAPLMMLEKKLKEIRLGRRNEKIDYRYDDEIGQLVAQYNRTVDELEESARL
                     LARSERESAWKSMARQVAHEINNPLTPMKLTIQQLSRTKKMDKEKFNEYFDKASQMLV
                     EQIDNLSQIARAFSDFARMPEAKFERVELGELLKSEVEFFKHNYEKVDISFEGSKEPI
                     YTFADPKQLIQVFNNLIKNAIQSIPENRQGKIEVKISTSHQQIEIRISDNGAGIPEDI
                     RDKLFIPNFTTKSTGMGLGLAISKNIIEVTGGNISFTTQIDKGTTFIVTLPKA"
     gene            complement(21713..22186)
                     /gene="rlmH"
                     /locus_tag="PLZ54_00860"
     CDS             complement(21713..22186)
                     /gene="rlmH"
                     /locus_tag="PLZ54_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOJ65524.1"
                     /translation="MKFVLINIGKTLDIHLSSLQEQYQNRLKHYLNFEVINIPELKNT
                     KNLSSSEQAAKESDLILKKLEIGDDVILLDERGTEFSSVEFADFLSKKMHSSNKRIVF
                     VTGGAYGFSEDLYRRANNMVSLSRMTFSHQMVRLIFVEQLYRAMTILKGEPYHHA"
     gene            22462..22887
                     /locus_tag="PLZ54_00865"
     CDS             22462..22887
                     /locus_tag="PLZ54_00865"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOJ65525.1"
                     /translation="MITKKRIANFKWLSMISLLMFISIQHSAQAQTSSIPNEIVSALS
                     NGDANRLSAYLNDNVELFIENKNDIYSRQQAVSILNDFFHRFKVSDFKVIHQGNRDNS
                     SFIIGILSTATENFRVYILTRKSDNKTIILQLRIETSNE"
     gene            22880..23755
                     /gene="nadC"
                     /locus_tag="PLZ54_00870"
     CDS             22880..23755
                     /gene="nadC"
                     /locus_tag="PLZ54_00870"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOJ65526.1"
                     /translation="MNEDYKQLIPLWFAEDIGDGDHTTLACIPETALGKSQLIIKENG
                     VIAGVEVAREIFKTFDPTLKMNVFITDGTEVKIGDIAFNVEGKIQSLLQTERLMLNVM
                     QRMSGIATRTRQYVKALEGTKTRVLDTRKTTPGLRFLEKEAVRIGGGVNHRFGLYDMI
                     LLKDNHIDFAGGIKEAILRTKEYLKNKGKNLKIEIEVRNFDEIEQVMAIGGVDRIMLD
                     NFNIENTKKAVEIIGGRYEIESSGGITFDTLRQYAECGVDFISVGALTHSVKSLDMSF
                     KADIPNLIEGIHKIF"
     gene            23792..24340
                     /gene="rfbC"
                     /locus_tag="PLZ54_00875"
     CDS             23792..24340
                     /gene="rfbC"
                     /locus_tag="PLZ54_00875"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOJ65527.1"
                     /translation="MEIIETPIPDLLIIRPKVFEDARGYFYETYNEKSFMEAGLDIHF
                     CQDNQSKSVYGVIRGLHYQLSPCSQCKLVSAIQGTVWDVAVDLRASSPTFGKWFGIEL
                     SAENHLQFLIPKGFAHGFSVLTETAIFAYKCDAFYNPSLERGILYNDPQLAIDWKIPE
                     DKAIVSAKDAKHPIFSKAEKNF"
     gene            24383..25252
                     /gene="rfbD"
                     /locus_tag="PLZ54_00880"
     CDS             24383..25252
                     /gene="rfbD"
                     /locus_tag="PLZ54_00880"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOJ65528.1"
                     /translation="MQTILITGSKGQLGNELQKAAKRFPNFRYIFTDVDELDICNKSA
                     LDNFVKENRIEIIVNCAAYTAVDKAEEEVEKCFAINAQAVKNIGEIAETYQIKVVHIS
                     TDYVFDGKSYKPYTEGMEVSPDTVYGKSKLQGEKFLQSVCNQCIVIRTSWLYSSYGNN
                     FVKTMLRLGKERNSVKVIFDQIGTPTYAADLAEIILKILSQNNFISGIYHFSNEGVCS
                     WYDFATAIFKIAHVNCLVHPIETKDYPAPAPRPYYSVLNKSKIKNTFGLSIAHWEESL
                     EKCIEILKSEYSL"
     gene            25427..26149
                     /locus_tag="PLZ54_00885"
     CDS             25427..26149
                     /locus_tag="PLZ54_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ65529.1"
                     /translation="MGRAFEYRKATKMKRWGNMARVFTKLGKQITIAAREGGPDPDMN
                     PRLRVLFQQAKKENMPKENVERAIKKAVSKEEADNYKEVIYEGYAPHGIAVIIETATD
                     NPTRTVASIRSYFNRYGGSLGTSGSVQFLFDHKCVFRIAPKSNLSIDDLELELIDYGV
                     DEVIQDDEENNIVLYGDFQHYSSIQKYLEENNFEIFSAEFEWIPKETKELNDEQKAQV
                     LKLIEKIEDDEDVQNVFHNMAE"
     gene            26259..27407
                     /locus_tag="PLZ54_00890"
     CDS             26259..27407
                     /locus_tag="PLZ54_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOJ65530.1"
                     /translation="MDISVLFPALASLTWQHILMILIGLTLIYLAIFKEYEPTLLLPI
                     GFGTILVNIPLSSAINQIDGKTGEIIEGILSVFYEGGIATEMFPLLIFIAIGAMIDFT
                     PLFNNPHLLLFGAAAQFGIFMTMFIATLFGFDLKEAASIGIIGAADGPTSIFVASRFA
                     PHLLGPISVAAYSYMALVPIIQPPVIKLLTSKKERLIRMQYSSKKVSRKALIAFPIVV
                     TIISGLIAPASLSLIGFLMFGNLIRECGVLNKLSQSAQNELASLVTILLGITIASTMT
                     AERFVTFGTLTIIALGLLAFVFDTFGGVIFAKLLNLILPAKKKMNPMIGACGISAFPM
                     SARVIHTMGLKEDPHNFLLMPAVSANVGGQIGSVVAGGLILTLVNLFA"
     gene            27420..27557
                     /locus_tag="PLZ54_00895"
     CDS             27420..27557
                     /locus_tag="PLZ54_00895"
                     /inference="COORDINATES: protein motif:HMM:NF040909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG-related small transporter subunit"
                     /protein_id="HOJ65531.1"
                     /translation="MINNVEAALEIGAIGITGIFIFMLIFYAAIKLILKLFPEKENKE
                     E"
     gene            complement(27679..28497)
                     /gene="panB"
                     /locus_tag="PLZ54_00900"
     CDS             complement(27679..28497)
                     /gene="panB"
                     /locus_tag="PLZ54_00900"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOJ65532.1"
                     /translation="MSVHNEDNRKVTTRRLFEMKQRGEKISMLTAYDYTMANIVDEAG
                     IDVILVGDSASNVICGNTTTLPITLDQMIFLAKSAIRGIKRALAVVDMPFGSYQGNSH
                     EAIASAIRIMKETHADGIKMEGGEEIIESVKRIIGAGIPIMGHLGLMPQSINKYGSYN
                     VRAQEEEEAEKLIRDAHLLEDAGCFALVLEKIPAKLGTQIASELSIPVIGIGAGGGVD
                     GQVLVIHDMLGLTQGFSPRFLRRYANLREQMNQAIQHYIADVKSSDFPNEKEQY"
     gene            28713..29177
                     /locus_tag="PLZ54_00905"
     CDS             28713..29177
                     /locus_tag="PLZ54_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOJ65533.1"
                     /translation="MSKITIHSYQELEEYVGKELGVSDYLTITQEQINKFADATLDYQ
                     WIHVNAEKAKQESPYKNTIAHGYLNLSILPYLWGQIMEIRNSKMTLNYGIENARFQQA
                     VVVNDQVRAKAKLKSLVNLKGIAKAEIYTVLEIKNSNKPALEGTFIFLYHFE"
     gene            complement(29186..30862)
                     /locus_tag="PLZ54_00910"
     CDS             complement(29186..30862)
                     /locus_tag="PLZ54_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005821705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HOJ65534.1"
                     /translation="MEWFLSLLLNDGIAHTILLFAFVISLGILLGKIKIFGISLGVTF
                     VLFVGLFVGHLGLSVNPEVLEFIKNFGLILFVYSIGLQVGPSFFSSFKKGGMLLNLLS
                     TMIVLLGVGIVIALYYLLHGKVSMPMLVGILSGSVTNTPGLGAAQEALRHVFGSSPGA
                     EIPPIALGYAVAYPIGVLGIIFSMVLLRIIFKVDFHKETEQIKKASEVDEEQVEKISV
                     QVKNEKVFNHTVTDVKRAVGRPFIVSRLMRNGEFFIPQGDSMLYKDDILLIVASSRDI
                     ERITSYIGEKVEMDWKISEEKLVSRRIVITHGKINGKTIGSLKLRTIYGVNITRVNRS
                     GVDLLGTPDLVLQVGDRVMVVGELDAIEKVEKLLGNTLQKLNEPPIITIFVGILLGVL
                     VGSIPFFVPGIPVSVKLGLAGGPLIVAILLGRFGYKLKLITYTTTSANYMLREIGISL
                     FLASVGIGAGEKFVETVISANGLLWMGCAAAIALLPLLIVGIIGRKFLKINYFTLIGL
                     LAGSTTDPPALAYSNSIAGSDIPAVAYSTVYPLTMFLRVVSAQILILAFL"
     gene            31084..32280
                     /gene="hisB"
                     /locus_tag="PLZ54_00915"
     CDS             31084..32280
                     /gene="hisB"
                     /locus_tag="PLZ54_00915"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HOJ65535.1"
                     /translation="MVHFRNKVHYKHINKINMEENTGKTVKKRVLFIDRDGTIIVEPS
                     ETFQVDSLEQLEFLPGVIRNLYFIRQRTDFEWAMVSNQDGLGTSAYPEENFEKVQSKM
                     LKILENEGIIFDKIFIDKSLPEEKLPTRKPGTAMLKEYFSANYDLENSYVVGDRITDV
                     ELAKNLGCKAILLNEDPEILQESKLKKVCVLQTTSWDEIAAFIFAGERTATIQRTTKE
                     TDIFIHLNLDGSGKGNISTGLRFFDHMLEQIARHSGCDLTIQVKGDLEVDEHHTIEDT
                     AIALGEAFAKALGDKRGIERYGFCLPMDDSLCLVAIDFGGRAWLTFDAIFKREYIGDF
                     PTEMIFHFFKSLSDAAKMNLYVKALGENEHHKIEGIFKALAKAIKMAVKRDIYQFELP
                     TTKGII"
     gene            32348..33925
                     /locus_tag="PLZ54_00920"
     CDS             32348..33925
                     /locus_tag="PLZ54_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HOJ65536.1"
                     /translation="MIFLLIVQFPLHAQIFPDQPRLVIGIVVDGLQEKHIDLLWQRFD
                     ANGFKKIIEKGTAFENMEYNIVSAGNASDIATLMTGTTPYYHGVTGNNFYSRKQGNVQ
                     SVIFDENEPGIGTKQTVSAHQLLASTITDELMLTYPKQSKTYAIAINAEDAIMLGGHT
                     ANSAMWLDDINMKWVTTSYYTDGLAASADEMNVNGQIEQYASSVWEPMYSPNTYLFPS
                     ANENKKNGFQYFPNSKRKGSNLPILKNTPNANALVADLALKIFEKENLGQDTYPDMLL
                     LQFNVKTPYEKTFALKTAEKEDMYLRLDKDLQYLFQKIDSKIGLSNTLIFILGTQTSA
                     HTPLELGENKIPAGYFNGRRSMALLNTYLMVLYGQERWVEGYYGKNIFLNRQKIEEKK
                     INLQEIQQVVADFMHEFEGIQSAYTSYQIVNLTDPSNREMSKLRNSFQKSSKGDVIIT
                     LLPGWLELDDNNNPIGESNAVISSTPVYFYGWNIEPKKIAAIHSIIDIAPTICKILKL
                     PFPNATTGKPLEEIINK"
     gene            33944..35119
                     /locus_tag="PLZ54_00925"
     CDS             33944..35119
                     /locus_tag="PLZ54_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOJ65537.1"
                     /translation="MKKYDFDEIIDRTNTNAVKIEACRSMFGTSNVIPLWVADMDFRT
                     PDFILDSIRKRCEHPILGYSVPPKDYFPSIMNWLKENHGWEVKKEWLGFMPGVVPGLS
                     FAVQQFSAENDEIVVQPPVYFPFMHVVEKNNRKLIFNPLKEVDGKFQMNFDDLEKKIT
                     SKTKMLILCNPHNPGGRSWGKETLQQLAEICAKHHLIVVSDEIHADMTLPGNVHVPFA
                     SVSEQAANISLTYMSPSKTFNIPALQTSYFIIPNEELRNGLKNFLDRSELHNGNIFAY
                     LSTIAAYTEGKWWKMQMLDYVQGNIDFVIQFLQNEIPQIKPMKPEASFLIWLNCSDLG
                     MNEEELFQFFVHKAGLGLNQGITFGPGGEQHLRLNVACPRSTLEKAMNQLKNAVRNL"
     gene            35130..35807
                     /locus_tag="PLZ54_00930"
     CDS             35130..35807
                     /locus_tag="PLZ54_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HOJ65538.1"
                     /translation="MWGTLVNALAVIVGGSAGLLLNKKMPQRIQTIYFQVIGLFTIGI
                     GITMVVGMKHILLIVMSLVIGVLIGEWMHLESRLEKWSESLKKRFQIGSEQFSEGFVT
                     AFLLFCIGSMTILGSIQEGLTGSPDLLFTKSLMDGFSAIILASAFGVGVIFSSIPLLI
                     FQGGLTLLAMAASSFFTPDLINGISNIGGILLIGLGINILEIKKLAITNMLPSLVVVI
                     LLIWGFA"
     gene            complement(35893..37482)
                     /locus_tag="PLZ54_00935"
     CDS             complement(35893..37482)
                     /locus_tag="PLZ54_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOJ65539.1"
                     /translation="MKTKITVLLTLMLFCMQAIFSQNQDTLSSHLILFYELNGNGKNS
                     VSDNNHLKLKSQPGNYYYKWTTGKDGKDSTAVVFDGNAQGLKTHLDISPQNLPEITYT
                     AWVFGRPDGFLFGSTLPVNNEKKFSSRSLQISKGAVICGFNKLDSLKNPYFASLRSAT
                     LKKDKWNFIAMTANANDSTLRLYVNDEYYESKPVVFTKKSNFLIIGNTQNSPYNRAFK
                     GQVDEIRVYNKALSSDELSSISGISFADAQKRMERNETIQKVLLAGLIIFLFVSVIYM
                     IVVLITEKRYKPIKTANNTSFSGDSKNARIDSEKEDANRLASECVEEAFDTWEPVSEE
                     NGQIFRSPTKRKHFKTTYRALNKAKSYQPTDNEVIDRMNDLGGLLNKLSKRKFYGNWF
                     LAVLSLIFPVVYFLLAKNHLDTKGTIGAMVMFLPTVAYILSNFAPNYVVANRKHNSGG
                     VFGKIIAFIMGAAASAVAVDYYTEKTWSDGSKTTEYDTGSNAMQLVIWAVLIIAAILL
                     SMILVIIAATIAFFRNYVFYL"
     gene            complement(37668..40829)
                     /locus_tag="PLZ54_00940"
     CDS             complement(37668..40829)
                     /locus_tag="PLZ54_00940"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOJ65540.1"
                     /translation="MLKQYLNSLQATFNQGDAREESYYGHLKDLLLNYTDERQTNKVD
                     ITVLPKPTEAGNPDFRIWDGKNHITGYIEAKDPSVTNLDRIEVSGQLKRYRETFPNVI
                     LTNFYEFRLYRNGELIKQVLVGRPELARKLKTVPPVENETAFFDLLDTFFSFSLPHVR
                     NAQSLATELAKRTRFLRDEVVSIELDEEEKRGKKAILGFYEAFRKYLIGTLTKEAFAD
                     LYSQTLTYGLFAARTRSDNSFNRELAFKYIPNTIGILRDVFRFISLEDPPKPLQVIVD
                     DIAEVLSVTDVRKILEDYFEEGKGQDPIVHFYETFLTAYDPSIREKRGVYYTPEPVVR
                     YIVRSVNELLKTHFDMPDGLASKNVTLLDPAAGTLTFPAEAVKLAVKEFTEKYGSGGR
                     NNFIRNQVLENFFAFELMMAPYAIGHLKMSFLLEELGYKLNDEERFKLYLTNTLEMDE
                     LEQISIPGLSSLSEESHLAGKIKKEQPVLVILGNPPYSGISANINEWTEKLLKQNIDG
                     AQSYYEVDGKTLGEKNPKWLQDDYVKFLRFAQWKIQKAGFGVVGMITNHSYLDNPTFR
                     GMRQSLMKTFNEIYILDLHGNSLKKETAPDGGKDENVFDIRQGTAIAIFVKQKEKSDC
                     KVYHAELFGKRQSKYDWLDAHQLEIKNYQLLKPESPWYFFIPRNTQNIKYYLKWKQIN
                     EIFPVNGVGITTARDNFVIDFRKEALLNRIRLFKNSKFSDNELYQFFHINKKIGWNIR
                     KAWNMLQDIPDNELENFIFDFIYRPFDHRYIFWHDSLVWRTVKQIMSHILKENLSITT
                     VRQVKAGETWQHSLVSDKLIESCYISNKTSEICYAFPLYLYKEHTPKKSRTQYMMMLF
                     EPETEYGNSNKEPNIDKKIYQTLNQNYGHELTPEEILYYIYAVFYSNIYREKYAEFLK
                     IDFPRVPFTTDYKVFSKMAALGNELVDLHLMKSSRLNTLVSKFQGEGENDVIEEIIFR
                     PEESRIYINSEKYFDNVTPQVWDYQVGGYRVLQKYLKDRKGRKMDDFVHYSQIISALN
                     YTIEIQQEIDTFYSEAEKEVIEF"
     gene            complement(41001..41087)
                     /locus_tag="PLZ54_00945"
     tRNA            complement(41001..41087)
                     /locus_tag="PLZ54_00945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41051..41053),aa:Ser,seq:cga)
     gene            41385..42722
                     /locus_tag="PLZ54_00950"
     CDS             41385..42722
                     /locus_tag="PLZ54_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOJ65541.1"
                     /translation="MKVEQVIDNLKRRFPNEPEYYQAVQEVLESIVDVYNAHPEFEKQ
                     NLVERLVIPDRILSFRVTWVDDKGNIQTNMGYRVQHNNAIGPYKGGLRFHPSVYPGIL
                     KFLAFEQTFKNALTTLPMGGAKGGSDFNPKGKSDAEVMRFCQAFMLELNKIVGPDTDV
                     PAGDIGVGGREIGYMYGMYKKLRNEANGVLTGKGLEFGGSLIRPEATGYGNIYFLMNM
                     LKTKGIHDLKGQVVAISGSGNVATYTAQKALQLGAKVITFSDSNGYIYDPDGIDEEKL
                     AYVFELKNVYRGRIKEYAEKYGCKYVEGARPWSEKCTIALPSATQNEITGEDAEMLVK
                     NGCIAVSEGANMPSTPEAVDVFLKNKILYAPGKAANAGGVSVSGLEMTQNSMRLNWTR
                     EEVDQKLQQIMQNIHEACVKYGTEADGYVNYVKGANIAGFMKVAKAMMAQGII"
     gene            complement(42789..>43630)
                     /locus_tag="PLZ54_00955"
     CDS             complement(42789..>43630)
                     /locus_tag="PLZ54_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ65542.1"
                     /translation="ALCWTMSALFFEKAGHKIGSLTVNVIRLSLAVVFLGITTFFTRG
                     FFFPTDATHYQWFWLGLSGIIGFFLGDLFLFKSYMVIGSRTAQLIMSLCPMLTALIGW
                     FFLDEKLSLKSILGIVICVTGIIIAISNRNLKLNIPFKGFLLAFGGALGQAVGLILSK
                     KGIGNYDPVAATQIRALFGLVCFALFITFLGRWKQVSAAFTNKSALKSVTVGTFFGPF
                     VGVALSMFAIQHTKTGIASTLMALVPVFIIWPSAVMFKERITIPQIIGAFVSIAGVSL
                     FFL"
BASE COUNT        13820 a         8280 c         8212 g        13218 t
ORIGIN      
        1 ctacgtgaca aaatcaattt cacagtacca cacgaaaggt tcgtgcggca gcgtgaaaaa
       61 attcaaaact ggttagacgt tgcatgcaac atctctactt ttatttctta tttcgtaatt
      121 caaagctcag aactctggac tcaaaactcg aaactatatt tttttggtaa aatgaaatct
      181 aaatttttcc tttcaaaaac tttccggtat aggaatttcc gcaatttaca attgcttcgg
      241 gagttccttc aaaaaccaca taacctcctt tgtcgccgcc ttcgggccct aaatcgataa
      301 tgtgatcggc acatttgatt acttcgggat tgtgttcgat aatgagaacg gtatggcctt
      361 tggcaatcag cgcatcaaaa gctttgagta aagttttgat gtcgtggaaa tgcagtccgg
      421 ttgtaggttc atcgaaaata aagagtgttg ggtccgattt ttcgtttgcc agaaaagacg
      481 ccaattttac ccgctgactt tcgccgcccg ataaggtatt ggatgattgt cccaatttga
      541 catatccgat accgacatcc tgcaatgact tgagacgttt cacaattttc ttttccaacg
      601 tattgtttga ttgtccaaaa aattcgatag cctgattcac agtcatttcc agcacatcat
      661 agatattcat tcctcgatag gtaacttcga gaatatcctg tttaaaacgt ttcccgtgac
      721 aatgttcaca ttccagtacc aaatcggcca taaattgcat ttcgattgtg atggttcctt
      781 ctccctgaca ttcttcgcaa cgaccgcctt ccgtattgaa agagaaaaag gaaggattat
      841 aacccatttg tttggccaat ggttgttcgg aaaaaagttt tcttatgtcg tcatacactt
      901 ttatataact caccggattg gagcgagaag aacgtccaat gggattttga tcgacaaatt
      961 ctacctgact gatcagttgc aaactgcctt gcaaaacacc aaactgtccg gtatgatcaa
     1021 ccactccacc ataatatttt ttcaaagcag cataaaatac atcctgaacc agtgacgatt
     1081 ttcccgaacc gctgacaccg gttaccactg tcatcacatt cagaggaaat tttactgaaa
     1141 tgttttttaa attgttttcc gtagctccga caatttctat gtaattgttc catttgcggc
     1201 gaagggtagg aacggggatt tcttccgtat gatacaaata tttcaatgtg taagaattgg
     1261 caatagtttc cttcgtattt tcctgtttgt caatatcgtt cagttgacca ttgaagacaa
     1321 tattcccgcc caatctgccg gctccgggac caatatcgat aaggtaatct gccgaacgaa
     1381 taatttcttc atcatgttct accacaataa ctgtatttcc caaattacgc agctgatgca
     1441 atacgttgat caacttttcg gtgtcgcgcg gatgaagtcc aatactcggt tcgtccaaaa
     1501 tatacagcga ccccaccaag ctgcttccca aagaagttgc cagattaatt cgctgacttt
     1561 caccacccga aagtgtattg gaaagtcgat tcaatgtaag ataacccaat cctacatcaa
     1621 gcaaaaaacg gatacgattt tgaatttctg tcaacaaacg actggcgatg gctgcgtcgt
     1681 gtccgtcaag cgaaagttgt tcaaaaaaat cgtataattc ttccaccggc atcaacacca
     1741 attccgtaat gggttttcca ccgacgcgca cataagttgc ttccttttta agtcgagttc
     1801 ccttgcaagc cggacaggtg gtttttcccc gataccttgc cagcatcacg cgatattgaa
     1861 ttttgtactg attttcttcc agaaacttga aaaagctgtt caatccttca aaatattcat
     1921 tccctgtcca aagaagttgt cgttgacttt cagtaagttc gtaataaggt ttatgaatgg
     1981 ggaaaccaaa ttttggtgca gcctgaatta accttttttt ccattcactc attacttcgc
     2041 ctctccagca tacaatggcg tcttcgtaaa cggaaagcga tttgttggga acaacaagat
     2101 cttcgtcaat gccgataatt tttccaaagc cttcacacac cggacatgca ccaatgggac
     2161 tgttgaagct gaaagtgtgt tcggtaggaa tttcaaatgt aatgccgtcg gcttcaaaat
     2221 tttttgaaaa atgtagcagt ttttctcctt cttccgtatt cactttcaga atacagtttc
     2281 cattgccttc gtaaaaagca gtttccaccg aatccgtcaa acggcttatg gtggatgggg
     2341 aatgatcaac caccaatcgg tcgataacca aatatatttc agtgtttttt tccagcaatt
     2401 cggggcgaag cagcaaatcc tgaattctga gcatttcgtt gcccgcttcc actcgactga
     2461 atccctgcat ttggaaattt cgaagctttt cagtcaaatt gttttcttca ttcaaccgaa
     2521 tgggaatgag taaagtaagt ttggtatcgt cgggatacga catggctgct tccaccacat
     2581 cttctgcctc gtgaattttt accaattctc ccgaaattgg cgaatacgtt tttccaatgc
     2641 gcgcataaag caatttcatg tactcgtaaa tttcggttga agttccaacc gttgatcgcg
     2701 gattgcgagt gttgaccttt tgttcgatgg caatagccgg cggtatgcct tttatgtaat
     2761 cgacttcagg tttgctcatt cgtcctaaaa attgtcgtgc ataagccgaa agactttcca
     2821 cgtatctgcg ttgtccttcg gcatatatgg tatcgaaagc taaagatgat tttcccgatc
     2881 ccgataagcc ggtaactacc accaatttgt ttctcggaat ttcaacatca atgtttttaa
     2941 gattgtggac acgtgcgcct ttaacaataa tatttccttg tttggacatt caactgctcc
     3001 ctaaaaatta aaataaacaa tagaaaatca gattgttatt taaaaatttt tctgattatt
     3061 cttcagtaaa cgacgatttt taaataacct gcaaagatac aatttattga gcggatgagt
     3121 aatagatgca gataaaattt attgaatata gttattagct gagtcatatg gaattttatt
     3181 gttttaattt tcgaatgaga aacaaaacac tgaaattgtt tcatatagcc aattgattga
     3241 gtgaattatt aataaaaaat aaaagattat tttttaaaaa ttaaaatatt ctgtacattt
     3301 gctcaattaa acgattcagt ataaaataaa aactaaacgt atgaaattca tagtttccag
     3361 cacagaactt ttgagccatt tgcaaacgat tagcagggtt atcaacacca agaatacttt
     3421 gcctattctg gatgatttca aattttctct cgaaggcaat actctgacga tgactgcttc
     3481 cgatgttgaa acaaccctca tcacttccat acaggtggag gaatctgaag gagaaggtca
     3541 agtagctatt gcttctcgtt tgttactgga tacactgcgc gagttttcgg aacagccgct
     3601 gactttttcc attaacgaat ccaatttgag cctcaccatc accacttcca atggagctta
     3661 taattttatt ggtgagaatg cggatgaatt tcctcgtttg cctccatttg aagaagaacc
     3721 ttgttctttt gaactccctg ttgacaactt gctttcggga atttccaaaa ctttgtttgc
     3781 agctgctgcc gatgatttgc gtgaagtcat gaatggtatt tattttgatt tatctcctaa
     3841 agaactcacc attgttgcaa ccgatgcaca caaactttcg cggtataaaa ccacaaatgt
     3901 gagtacttct ttggatgaag atgaagtaaa aagttttgta cttcccaaaa aaccggcaaa
     3961 cctcttgaaa aacatcctgc caagagaaaa gggtaacgta aaaattaggt tcgacaagaa
     4021 gtacatccat tttgaactgg aaaactatac gatggtttgc ctgcaggtgg ccgggaaata
     4081 tcccaactat aacagtgtca tccctttcaa caatcccatt aaaattattg ttgaccggct
     4141 aatgttgctt aattccgtca gaagggtttc cgtattttgc aaccaggcaa ccaaccttat
     4201 aaaactggtt ttcaacgaac agcaggtaaa tatttcggcc gaagacattg atttttctat
     4261 ttctgccgaa gaaaccattc cttgtcaatt ggaaggaaat gctatcacta tcggattcaa
     4321 agctacttat cttatcgaca ttctttccaa tatcgattct tctgacgttg taatcgagct
     4381 ttccgatcca agtcgcgctg cattgatttc ccccctcgaa aacgaagaaa acgaagaagt
     4441 cttaatgctc cttatgccga tgcttttaat ggattacgat taaattcacg attttttcag
     4501 tcggattatt tttaaggtct aaggtgaaaa aactttagac cttgtttttt cgattttagc
     4561 tttgttcaat ttttccaaaa aaaaaatttc atggaattaa atttaaaaaa accgattgtt
     4621 tttttcgacc tcgaaacaac aggtatcaac attgttaccg accgtattgt ggaaatttcc
     4681 tatcataagg ttttccctaa cggaaaagaa cttacgaaga ctaaacgtat taatcccggg
     4741 atccctattc ctgcctatgc aactgctgtt catggcatta ccgatgccga tgtggccaat
     4801 tgtcctactt ttaaggaagt agccaaagaa atagcggccg attttgaagg aagcgatttg
     4861 gcaggttaca actccaatcg gttcgacatt ccacttttgg cagaagaatt tttgcgtgca
     4921 gaagtagata tagatttgat gaaacaccga tttatagatg tacaggttat ttttcacaaa
     4981 atggagcagc ggactttgtc ggcggcttac aaattttact gcaacaagga tttgaatgat
     5041 gcccacagtg ccgaagccga tgcaatggct acctatgaag tgctgaaagc tcaattggaa
     5101 cattatcccg aattgaaaaa cgatgtagat tttttatccg attttactac ccagaacaac
     5161 aatgtggact ttgccggacg cgtcatttac aacgaaaaag gagaagaaat tatcaatttc
     5221 ggtaaataca agggcaaaaa ggtggaagat gtacttaaaa ccgatatggg atactatggt
     5281 tggattatga acagcgattt ttcactccat accaaaaaag tttttaccaa cattaaactt
     5341 cgccaattca acaacaaata attgcataaa gttaatttgg attgcaaaaa atcttcttat
     5401 attcaggaaa atattcaaaa atttaatggt tcccgatact tggtgcaaat tgatgttaat
     5461 tgcatatctg cccgtttttc agaaaacgac ttcctgatat taaatctgct cgttgtcaac
     5521 taaagcattt catctttttg tgtgaaaatt gaaaattatc gtgtaatttt gtcttttgaa
     5581 actaaaacag ccaaaaatga ataaaattgt caacaaagaa tatttgtccc ccaacgtggt
     5641 gaagtttgaa gtggaagctc cattgatagc caaagctcgc aaagcaggcc attttgtgat
     5701 tgtacgggtt ggagaaaaag gagaacgcat tcccttaacc attgccgatg ccaatacaga
     5761 aaaaggaacc attactttga ttgttcaaaa aattggtttg tccacttcca aactttgcga
     5821 attgaacgaa ggcgattgca taaccgattt ggtaggtccg ctgggaaaag cgacccatat
     5881 tgataatttt ggaacggtag tttgcgcttg tggaggtgta ggaacagctc cgatgcttcc
     5941 catagccgaa gccatgaaaa aagcgggtaa tcacgtaata acagttttgg ctgcacgtac
     6001 aaaggagtta atcattttgg aggaacaaat gagaaaattt tccgatgaag taatcattat
     6061 gaccgatgat ggctcttatg gcaacaaagg tttggtaacc gatggtgttg aaatggttat
     6121 aaagcgccaa aaagtagatt tatgtgtaac tatcgggccg gcaatcatga tgaaatttgt
     6181 gagtgaactg acaaaaagat ataatattcc tactattgct tcgctcaata ccatcatggt
     6241 agatggaaca ggcatgtgtg gcgcttgccg catttccgtt ggaggaaaaa caaaattcgt
     6301 ttgcgtggat ggccctgaat ttgatgctca tcaggttgat tttgatgaaa tgctgatacg
     6361 cttaaacgga tacaaggata tcgaaagaga acaattacac aaaatatctc ctaacaattg
     6421 attccccagc aaaatatttt tgttgtagaa acaaattact tttttgaaaa ttttttaagt
     6481 ccgaagaaat gacaaatttt aatgaggaaa gaatgcaacc ttggcgggtg gaactgagaa
     6541 attccatgaa gccgaaagac agaatggcaa ttgccagagt aaaaatgccc gaactcgatc
     6601 ccgaatatcg cagccacacc atgaaagaag aagtcaattt gggtttaacg cccgaaatgg
     6661 ctttgatgga atcgaagcga tgcattgatt gtgccaaccc aacttgtgtt caaggttgcc
     6721 ctgtggctat caatataccg aaattcatca aaaatattga acgaggagaa tttttggaag
     6781 ctgccaaaac attaaaagaa acttcagtat tgccggcagt ttgcggacga gtttgccctc
     6841 aggaaaaaca gtgtgaagca cagtgcattc atttaaaaac aaaaaacgaa gcggttgcca
     6901 ttggttatct cgaacgtttt gctgccgatt acgagcgtga aacaggcaac atatccattc
     6961 ccgaaatagc cgctccgctt cacaaaaaag ttgctgctgt aggttcgggc cctgccgggt
     7021 tggcttttgc cggcgacatg gcaaaaatgg gttacgaagt ggtgcttttt gaagcattgc
     7081 atgaaattgg aggcgttctt cggtatggaa tacctgaatt tcgtttgccc aacaaaattg
     7141 tagatgccga aattaggaat ctgattgcta tgggtgtaca ttttgtgacc aactgcatta
     7201 ttggcaaaac cattactata gacgacttac tggctgaaga ttataaaggt attttcattg
     7261 gaagtggtgc cggattgcct cgtttcatga atataaaagg cgaaaatctg gttggcatca
     7321 tgtcatcaaa cgaatacttg acacgagtca atttgatgga tgcagccaat cctgaatcgg
     7381 atacacctgt tataaaaggg aaaaaggtgg ccgtaattgg tggtggaaat acggcaatgg
     7441 acgcagtaag aaccgccaga cgtcttggcg cagaaaaagc catgatcgtt tatcgccgaa
     7501 cagagcagga aatgcctgcc cgtgttgaag aaatcaaaca tgccaaagaa gaaggaattg
     7561 agtttatgac tttacgaaat cccattgaat actatggcga taaaaacgga agagttaccc
     7621 acatgctgct tcaggttatg gaattgggcg aacccgacgc ttcgggacgc cgctctccgg
     7681 tagaagtcaa agggaaaacc gaactggtgg aagttgatga agtaattgtc agcgtaggag
     7741 tttcacccaa tccgctcatt ccacaatcgg taaagggact ggaagttacc ggcagaggaa
     7801 cattggtagt gaacgaagaa accatgcaga cgagcattcc ctatattttt gccggcggag
     7861 atattgttcg tggtggtgct acggttattc ttgctatggg cgacggaaga aaggctgcaa
     7921 aagggatgaa tgaatggatt aggagcttta cttgaaattt gaataagatt taaaaaatat
     7981 tttttaaaaa actttttcct attaattttg tttccattta caaagttaaa tttaaaatct
     8041 tactaaaatg gaaaatgaag tttcagtgaa agtagccaaa ggaggccctt acatcgttag
     8101 tggtaatttc aagcttgttt taccttcagg agaagaaaaa gaaatgagtg gtaaaacagc
     8161 actttgtaga tgtggacaaa gcaaaaataa acctttttgt gatggttcac atcatgatac
     8221 agagttcgat aaataattga agaattgttg attttattaa aggaaacagg gataaaaaat
     8281 tgtctcctgt ttcttttttt tctttctgag gatgttgaaa cattaacttt atttccgaat
     8341 aattttttgg tgcaatttcc gattaatgtt aattttgttc tttctaaaat ttattgaatg
     8401 gacaaattac gatttcaaag ttttggtagt ggcagtagtg gaaattgtta ttacattgga
     8461 aatgccgaat atggtttttt aatagatgca ggcatgggat cgcgaactat ccagaattgc
     8521 ctgaagaata taggtctgaa tttcgaaaat attcagggcg tgtttatcac tcatgaccac
     8581 gtcgatcata tacgttcatt gtgggcattg gaaagaaaat ttcatcttcc ggttttttcc
     8641 acccgaatga tttcggaggc aatcaatcaa tattattatc gtgaaaagaa tattaatttg
     8701 cctaaacgaa gatatatcga gaaaaatgaa acacttgata taagggattt ttcctttacc
     8761 gcctttgaca tttctcatga tgctacggat aatgttggtt acagcattga atataaaggg
     8821 aaaagaatta catttgcaac agatttgggt tgtattggaa aaacagccgc atcctatttg
     8881 attttgtccg attatttagt tatcgaagcc aattatgatg agcaaatgct ccgaaatggg
     8941 aaatatccag attatttaaa aaaaagaatt tcttcttcca gagggcattt gagcaacgat
     9001 cagaccggtt attttctttc tgaaaactat acagagaaaa tgaaaaatat atttctttgt
     9061 catctcagca aggaaaacaa ctctccacaa attgctctga aaacgatcag agattatatg
     9121 gaaaataaaa atatttcggt aggcaaggat gtggacgtgg tagcgctgaa tcgactaaat
     9181 gcttccgaat tgtacgaatt ttaaatttat tcctctgcaa attaaaacta tgggtgaaaa
     9241 tttagttatt attcctactt acaacgaaaa ggaaaacata gaaaatatta tttatgctgt
     9301 ttttcgtcag ccccatgatt tccatttgtt gattgttgat gatgcttccc ccgatggaac
     9361 agcagagata gtgaaaaact tgcaacaaaa attccctgat cgattgttta tgattcagcg
     9421 cccgggaaaa atgggactcg gaactgctta tattaccggc tttaaatggg ctttgcagca
     9481 cgattatcag tatatttacg agatggatgc cgatttttct cataatcccg acgatttacc
     9541 ccgattatac gcttcttgcg ctctcgacgg agcagatatg gctattggtt cacgatatct
     9601 cacaggcgtg aatgtattaa attggccgct aaagagagtt ttaatgtctt attttgcttc
     9661 ctattatgtt cgtatcgtta ccggtcttaa agttaccgat actacagcag gatttgtttg
     9721 ttataaaaga aaagtgctgg aaaccattga tctggataaa attcgtttta aaggttatgg
     9781 gtttcagata gaaatgaaat ttacggctga aaaatgcgga ttccaattaa aagaaattcc
     9841 aattgttttt attaatcggg agtacggcac ttcaaaaatg agcagtggta ttttcggaga
     9901 agcttttttt ggtgtaatcc aattgaaaat tgaaagctgg ttcagggatt tccctaaaaa
     9961 agcaatcaaa tccagttaaa ttatttatta tgagtgaact gcaattgatt agaaatgcac
    10021 aaatagttaa cgaaggaaaa atattcatcg gttctgtgct cataaatgga gaactaattg
    10081 agtcagttca cgaaggaaag aataattatg gagagataga cccaaataag gttgaaatca
    10141 ttgagggaga caatttactt cttttgcccg gcgttataga tgatcaggtt cattttcgcg
    10201 accccggact tactcataaa ggtgatattt attctgaaag taaagcagct gtagctggag
    10261 gagttaccag ttttatggat atgcccaata cgcgtcctcc tgctgtaaat aatgaattgc
    10321 tcgaagaaaa atttcaactg gcagccgaaa aatcgcttgc caatttttcc ttttatattg
    10381 gagctaccaa tgacaatttg gatgaattac tgaaaataaa tcctcaacag gtttgtggta
    10441 taaaagtttt tatgggttct tctacaggga atatgttggt agacgacgaa aaaacattaa
    10501 atgccatttt ccgtgatgcc ccctgtctga ttgcaacaca ttgtgaagat gaattaacta
    10561 tccgcagaaa caaggaatat tatcaatcct tgtatggaga aaatttaccc gcccatttcc
    10621 attcgctcat tcgtaatgaa gatgcttgtt ttgcttcttc ggcgaaagca gtttcgttgg
    10681 caaagaaata taattctaga ttgcacattt tacatctttc aaccgaaaag gaaatgtctt
    10741 tgtttgataa ctctgtttca cttgaagaca aaaaaataac ggctgaagtt tgcgttcact
    10801 atttgtggtt ttctaataaa gattatgaac agttggggaa caaaattaaa tgtaaccctt
    10861 ccataaaatc agaaaccgac aaactttcac ttatacgggc gataaacaat aataccattg
    10921 atgtaattgc tactgatcat gctccacatt tacttcccga aaaggaaggt aactgtttga
    10981 acgcagcttc cggattgcct ttggttcagc attcattggt aaccatgttg caattggcaa
    11041 aacagggtta ttttaccatt gaaaaagtag tcgaaaaaat gtgtcatgcg ccagcccgat
    11101 tatttaaaat agagaaaaga gggtttattc gtccgggata ttacgcagat ataacattag
    11161 tacatcctaa cgatacttgg caggtttcca aacaaaatat tttttataaa tgcgggtggt
    11221 cgccatttga agaaatgagt ttcgactttc gggtactgaa aacatgggtc aatggaaata
    11281 aagtatttga tgaaggaaaa tttgatgagc aagtgagggg aaaacgattg ttttttcgag
    11341 tataaacaat tcttttcatc agaatataat tagttaatta tagagcaaat aaaatttgaa
    11401 accacaaata ataaaactat ggaattcaga gatttaaaaa agcagtacaa agttctgaag
    11461 aatgatattg atcaggccat gttggaagtt agtgcttcgg gcgcatttat catgggaaaa
    11521 cccgtcaaag aaatagaaca gcaattggcc gaatatgttg gcgtaaaaca ctgtgtttcg
    11581 tgtgcaaacg gaacagatgc cttgacattg gctttaaaaa catggggaat aaaagccgga
    11641 gatgcagttt ttgtacctga tttcactttt tttgcttcag ccgaagttgt ttctctcgaa
    11701 ggagctacac ctgtttttgt tgacgtggat gccgatactt tcaatatttc tcctgcatct
    11761 ttggaacgaa gcatagaaaa aaccattcaa cagggaaaac ttcggcctaa agtcattatt
    11821 acggtcgatt tgtttggact acccgccgat tattctgcta ttagaaaaat tgccgacaaa
    11881 tatcaactgt tgattttgga agatggagca caaggtttcg gaggagaaat taacggcaaa
    11941 aaagcttgca gttttggcga tatttccact acttcgtttt tcccggctaa acccttaggt
    12001 tgttatggtg atggcggagc agtttttacc gataacgacg aatgggcttc gctgatggat
    12061 tccttgcggg tacatggcaa aggttctttt aaatatgaca atgtgcgtat aggtatgaac
    12121 tcacgtctcg atacattgca ggctgccatt ttaaaagtga aatttgatgc tttcagaaaa
    12181 tatgaattga acgacatcaa taaagtagcc gatttttata cggaaaaatt aaaagattta
    12241 gtgaaaacgc ctttcattcc caaaggatat ctttccagtt gggcacaata caccattcaa
    12301 ttgcaggatg aaaagcagcg agatggcttg caggcttatt tgaaggaaca aggtattccc
    12361 acaatggtgt attatccaaa acccatgcat gagcaaacag cctatcttga tttgggcaat
    12421 ttatcaggtg atttctccgt aaccgaatcc ctatgccgca gagtactttc tctaccaatg
    12481 catccttact tgagtatgga agaaattaat aaagtctgcc aaacgatttt agattatctc
    12541 aaataaattt caactgttat ctctgataag actttaataa ttttatagca tttacgacaa
    12601 acagatttcc tgataatttt caagaaattg cttgtgttga gctgtttggt taaaaagatc
    12661 acgaatatgt tcatccattg tcagataaat gatttgccag ccggcagatg acaattcaaa
    12721 cagctgatct ataattttgg ggcgtcgctc gtaatcgcta tgctgaaaag catcgtccaa
    12781 catcagaaaa gccgtttcgt tgagtgctct ttgtgccaaa gttatgcgaa gagcaagcat
    12841 tacctgttct ttggttccgg tgctgagttc ttttagcagc cacgaattat tttcactcga
    12901 tatgattatt tcattatcgg caatttccag cttattgtat tttcccgtaa gccgataaag
    12961 caaattggta aaaatgccgg aattgatacc ggcagccaat tcttcatctt cctgctgttg
    13021 aagttgtttg attgttttgt aaagtacatt tgcggccaca atgtacgctt cgtttttttt
    13081 aagattagag agagtttcct tttctctttc gtaaagattt tcaaccactt cgttccatcc
    13141 tgtggtaaaa tcaatattta aacacgtgca aatttccgat ttcaacttgt cgattccatt
    13201 ttcgagttcg gaaatttttt cttttaaagt ttccatttcg ttttgaagtt tagtctgttc
    13261 ttccgccgaa aattcaattt cctgattttc ctgcacataa tcttgaggcg aaacaccgag
    13321 tcccttcaac tctcccgtta ggcccgaaag tttttccttc agttgcgttt gaaactgacg
    13381 tcgttccgta atattttgct cccatttgtt ttctggaatc tctgttccaa atatggcccg
    13441 aaagctttcc aaaatctcat ttttcaaagc tgtagtcagt tcggaaagtt gtcgaagcct
    13501 tgtttctttt tctccttttt gctcacgcca gatattaagc tcagtcattt tctttgaaag
    13561 tgagtccaat aaatttttgc tgtatgcaac acagggatca ttgggagaat aatcggaaac
    13621 atccacctgc aacatcatca gttcctgctt cagttgttct ttgtggcgat tcaactcgtt
    13681 gcgttgtttt tttaatgaag atagccgctc gtaaattttc gacatttcta cttcctgccc
    13741 aaaaatacca agcgtttttt cttttattgt ggctctcagt tccaaaattt tctgttcggt
    13801 tttttttcgg tcttcctgca tatagccaag ttcaacaatt tttttattga gcgcgtcata
    13861 aagagtgtcc agatttacct gattcagttc ctgatgaaat ttttcctgaa attcttgctt
    13921 gatctgattc cattccgaat ttcggagttt atttttgaaa gttttttgaa gacgaaaaac
    13981 gtaaaatgac aacgctataa atgaaccggc aaacagtatt gcgccaaaaa gaatgttttt
    14041 caacgctaaa agtgttccca aaccgcctgc tgcaaagaca acagataaaa ccatcaatat
    14101 tttgttgagc gtatcttttt ctgacgacga aaattgaagt tgttgataag ccgtgcggac
    14161 tttttccaac cagtcgaaat gttgggtttg atttattagc tcttcaagct ttggttcttt
    14221 gtcagatatt tcggtttgat aaagattgtg taactcaagg tttttttcga gttcagaaat
    14281 ttctttttca tcaacggcat cgatttgctt ttccaaagcg gaaatttttt ggttcagcgc
    14341 gtaagctgca tattgttttg ctgcaatttg ccgatcgaaa ttgtttttcg tttcatcaaa
    14401 atcttttgtc cgttcttcga gcgtttttat ttcgatactc aactgatttt tttgggattg
    14461 aagttctcta cagtttttaa tttttgtttc cagttcggaa agttcggatt cggaaatatt
    14521 ttccaattga tgatgaacat tttcaatttc agtggcatat tgccatgcct tgtagcgacg
    14581 agccaacaac agtttttcgt ttttcttctt taaaagacga aattcttctt tcagttcctg
    14641 aatttttccc gtttcagatt ggctggagac caactcgata aagtgccgga ttttattgag
    14701 attacttagc tcgtcgtaat aaattttgcc ttcaccctgt cgattaatgg taatgagatt
    14761 cccgtcaatt tttgaatttt ttatggtagg agatatcttt gctgccagtg tattcaatgt
    14821 tttttgtgaa gaaagcagtt ctttcagggt atttaagtcg ataccgttca aattgttctc
    14881 ggcgatgttt atttctccac ttttgacaat caataatttg gccaatgccg gcgaaagccc
    14941 gttctcatca gaaaaaagca catccagttt ctcgttcttt ttagttcggg gtgaaaaagt
    15001 tttttcctgt ccgttaaaac ccgaaacaat aattctgcca ttgccggctt cgcgcaattc
    15061 tccccactga cgagtgccgg aaaaaagcga ccgaatgata aattcggtca gaaaagattt
    15121 tccgtcttca ttttttccat aaatcaagtt aacatttttc atttcataat tgaaacgttg
    15181 gagcgggcct aaattttcag cagatatttt ttcgattcgg attcccatca ttacttgtga
    15241 ttaaacaaaa atcaatttta aagtttgttc cataatatcg tctttgccgg catagtcgaa
    15301 gtttggcaat tcgtcaatga tttttttaac tttttcgaac agcgaaattc tatcttcatc
    15361 ttttaaaatg ttcagttcgg tggtgtcgat tcctgtttca tcataaaaac cgatcccttt
    15421 ccgatgcaaa aaatcgttta caaaaccgga aatttttgct ttatcggttg taaaaccctt
    15481 aaccgatagt cgcaatttga cttttgaaaa atcttccggc aacagttgcc aattgtctat
    15541 catttgttcc agttgctggg ttaaatagga tatttcgtta tcggaaggca acacaacaat
    15601 attttcgaca aaataaatga ccgaagctgg caaaccgacc gaaaccagac tgttgtcatc
    15661 ggtgtcgaaa agcagccatc ttctttttcc cgtttcgttt acgtcgaggg gaaaaggaga
    15721 ccctgcatac caaacactgc ccgatgtgta aggtttgtgt atatgtccca gcacaacttt
    15781 tgagggtcgg aaagcagaaa cgctgcccga agacaagggc atgtaaattc ctttttcgta
    15841 gggattcatc tcgcgacttc tggaaacata atcaccatga ccaataagca cccaattttt
    15901 taattcttct tctccttttc tttgaataaa aaaattactc aaggcctcat ccattgaaag
    15961 tttggcatca taaggcaaaa aaacaaaagg tatggaaccc aaaattttta tttcgggaga
    16021 agatacaatt tccaagttgg aagccgtaaa atatttttgt tccaacagta agtcgtgatt
    16081 gccaggaatg accagaaaat gtttggacga atgaagttta caaaaagcat caaaatcggc
    16141 ataattatga aaatccttat cgaaaaggtc gccggcaacc acaagtgtgg agacatcaaa
    16201 atcgcgcatg gaatttacaa gagtttccaa agccatgtat cgctcgggcg tttcggcttt
    16261 ggttcgcaaa tgcacatcgg cagtaatggc tattttcatt atacgtttct gacaaaaagt
    16321 gtaacgacta aaggaaaaaa atcagtaaaa gattcactaa aaaattgtct tccaaaaaca
    16381 taagttattc gttaggtaaa ctattttagc acataaaatt agcaattcta ttcacatttt
    16441 caaaaaaaac ttgcagttaa actcaaatat aatttccaga aaaaacgtaa agaaacatgt
    16501 aaatggaaca gaaaaaatcc ctaattttgt agctttcagc aataaaaaaa ttggacgctc
    16561 aaaagaaaaa aatatcaaaa gtcatggcaa aaaccaaaac tatgtatgtc tgtcagaatt
    16621 gtggagccga ttcgcccaaa tggatagggc gatgtccttc ctgtggcgag tggaatactt
    16681 atgtggaaga agtggtgagc aaaggaaatg cggaaaaaat catcagtgga ggttttaccg
    16741 ttactgctca aaaacccgtt ttgttgagcg aagttgaagc tattgaagaa aaacgcatca
    16801 acactttcaa cactgaactg aaccgtgttt tgggaggagg attggtaccg ggatcgcttg
    16861 tattgattgg cggggaaccc ggaattggaa aatcgacact ggttttgcaa gtggttttgc
    16921 atctcaaagg aaagcgaacg ctgtatattt cgggagaaga aagcgtaaaa cagattaaat
    16981 tgcgggcgga acggttgaaa ttcgatgatc ccgattgtta tcttttgagt gaaacctcac
    17041 tggaacaaat ttttgttcat attcaaaata tacaacccga tttggtaatt gtggattcca
    17101 ttcagaccgt ttctaccgaa atgctggatg cttctcccgg ttcggtatcg caggttagag
    17161 aatgtgcagc cgcttttctg aaattttgca aaaccacttc cattccggta attttgattg
    17221 gacacatcaa caaggaaggc ggtctggcag gtccaaaagt actggaacac attgtggaca
    17281 ccgttctgca attcgaaggc gaccgacatt acatgtaccg tattttgcgt tcgataaaaa
    17341 accgatttgg cagtacatcc gaactcggca tttacgaaat gcgtcaagat ggtttgcgcg
    17401 aagttgaaaa tccttcagaa atgctctttt cgcaggatca cgaaggattg agcggcattg
    17461 ccattgcttc cgccattgaa ggcattcgtc cctttcttat cgaaacgcag gcgttggtga
    17521 gctctgctgc ttatggcatg ccacagcggt cgagtacggg ttttgatctt cgtcgactca
    17581 atatgttgct ggcagtgctc gaaaagcgtg tgggttttaa attgaatcag aaagatgtat
    17641 ttctcaatat tgccggcgga ttacgagtaa atgatccggc tattgatttg gctgtgattt
    17701 ctgcaatttt gtcttcaaat gtcgatattg ccatcgaaaa acaggtttgc actgccggag
    17761 aagttggtct ttcgggtgaa atacgaccga ttcatcgcat tgaacaacga attttggaag
    17821 ccgaaaaact gggatttaaa aaaatgattg ttcctgctaa taatttaaaa ggcctttcac
    17881 tcaaaaatat cgaaatggaa attataccgg tgaaaaaagt ggaagaggct tttaaaatat
    17941 tgttaaaaaa atgaggtgtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 ataatttttt caagctttcg gaagagtaac aataaaagtt gttcctttat ctatttgggt
    18121 agtgaaagaa atatttccac ccgtaacttc aataatattt ttagaaattg caagtcccag
    18181 ccccattcct gtagatttgg tagtaaaatt cggaatgaac aatttgtctc tgatatcttc
    18241 aggtatcccg gctccattgt cgctaattcg aatttcgatt tgctgatgag aagttgaaat
    18301 tttcacctct atttttcctt gtctattttc gggaatggat tgtatggcat tcttaatcag
    18361 attattgaaa acttggatca attgttttgg gtcagcaaaa gtgtaaatcg gttctttgga
    18421 accttcaaaa gaaatatcga ctttttcata attatgtttg aagaactcca cttccgactt
    18481 cagaagctct cccaactcta ccctttcaaa ttttgcttcc ggcatacggg caaaatcgga
    18541 aaatgcacgg gcaatttgcg aaaggttatc aatctgttcc accaacattt gagaagcctt
    18601 atcaaaatac tcgttgaatt tttccttatc catttttttt gttctgctca attgctgaat
    18661 tgtaagtttc atgggtgtaa gcggattgtt gatttcgtga gccacctgcc gtgccatcga
    18721 tttccatgcc gattcccttt cggaacgtgc caacaggcga gcactctctt ccaactcgtc
    18781 cactgtccgg ttatattgag caaccaattg gcctatttcg tcatcataac gataatcgat
    18841 tttttcgttt cttcttccca agcgtatttc tttgagtttt ttctccagca tcatcagtgg
    18901 agcggaaatc tgattgccaa taagcaaggt cagtataata accaataaaa tgataacaaa
    18961 atatatgtga acaatcaccg aaataaaact ttcaatttca ttctgaattt catcctgact
    19021 gaagaactga ggaacggcta tgtatccgat ttgcagataa tcgccattga aaaaatcggt
    19081 ataagccgtc aaataattca aatggcctat gttttcatac tgattgatat tggcatcttc
    19141 agtaaaatat ggctttggcg caattcggtt gctgatcaat tttttcgaaa aaatcaaagg
    19201 ctgtgaactg cccaccaaaa ttccgtcatt gttgtaaaca tgcacatcag tatggaaagt
    19261 ataggaaaga tcctgcaaat ccaaatttaa tgtctgggta tttttatcgg acaaatcttt
    19321 gttccaataa tacaaatttt gaagcgcctt ctgaatgtaa atttttttgt tctccagatt
    19381 ttgaatctgt tctttctcgt aattgttacg aatatagtca accgaagaat aaaaaattcc
    19441 gataaaactc aaaattaaaa gagcaatgaa tgaaatttgg aaccggttga taaaactcaa
    19501 agtaaatttc cttccttttg cgaaaaaatc atcaacttgc tgaagtaacc aacacaaagc
    19561 aaaaaaagcc agaaaagcat aacaaaaata caacaaatat gcgccttctc catattgaac
    19621 attctctgtc agaaccagat aagtattgcg gtcgggggca taaacataat gacgtcgatc
    19681 ggcataatga actacaaaat aattggaatt ctcgaacgga atccaattgg aaacatcggg
    19741 gtaaacaaaa tttccatttc tatagaccaa ctttcgatga tcgtaccggg caagcgaaac
    19801 attcaactgc gacaaaatgg aaggcggaga tgaaatcagt agattgggaa aactatagct
    19861 tctgaaattc tgacggggat agaattccat aaaataacaa gtagaatcat tgccggcagt
    19921 taggacagaa aaaactccca aatatgaaat attcttttcg ttcggtataa tgttgtagaa
    19981 atgagtctgt ttgattttta ttcctttttc ctgcaaaaat aactgatatt ccttgcacaa
    20041 atccgagttt tttgaaactg cattaatatg tacttcaaaa cggttccaaa aaccacgcaa
    20101 gtaactattg ttcaaatact cgcttaaaac ttccaccgaa tcggagtttc tcatcaactg
    20161 atttacaaag cggtcgcgac ttaactgggc atccaattct tccatcagaa tttctgtcat
    20221 ttggtcgttt tcaatggtac cattcagtga aagattttcg gccgcaattc tgtacttgtt
    20281 aaatttcttt tcattggata aaaccaccac atttccaata aaaaatatcg aaaagaacaa
    20341 aacataaatt atgagtttga catgtctttt tctggaaaaa tttacaatat aaaagccata
    20401 caagggtatc cagagaaata agtaggagaa aaaagaaagt ttccacattt cgggaaaaac
    20461 cttgaaagcc gccatcgcta aaagacaaga aaaaatcgca tcgattgcaa aggcaatttt
    20521 catataataa tgtctggtca gaaaagtatg aattttgaaa aacaacaagg caaatccaat
    20581 tccccacaga agcattaaaa tatgcatcca cacggtaaat atggaaaaat ttttaaagct
    20641 gagaataagt atttgcggcc cggaatgata aactactcct cgtaaaacat aataaaacaa
    20701 caaaaaatac aaaggcaaca ttagcagcaa tccgaaactt ctcaaaaaag aaacactatt
    20761 tcccaactga acatggcgat aaaacaaaaa aaccgtagaa aaaatgtaaa cagtaagcaa
    20821 ggtcaaatgt accgttgaat ttaaaagtgg cggaactgca tacaaataag gcgcaaaaag
    20881 cttgttggca agtaaaaccg aaggaaaatt aaaatacagc atcactgcca acaacgcacc
    20941 ggtaatcaaa acataaccac aaaaatcttt ccattgcaac gattttgtgc gcgaaagaga
    21001 aaatcctctt gcataccaca caaaaaacag caagaaaaac aaaagaaaag aaattaagcc
    21061 agccaaagcc caacgttcat catacacggg cactgtaggt tcggagagtg aaaacaaata
    21121 atcgccgtgt gaactgaaaa ctgcatactt atccaaattt gaacctccga caattccgac
    21181 gtttttgttt aaattgaaac ctttggcaaa atcattgatc agttgatcgt tttcgtaagg
    21241 aaaattattc tttatcttaa tcaatgccaa caatttcaac gaatcggcag aaagcgtttt
    21301 gctcacacaa tatgcattgg gcaactgaac aaaatgaagc tcaacgctgt cgttcaacga
    21361 aatactgctg acgtccaaat gatgatccga ccaaaaaatc agctcattgc cccgataaac
    21421 ataaaaagat atatcatcgt cgggaaaaga aaaattaatc agttcgttaa ccgaatgatt
    21481 atcaagcaaa ttactcattt gcttcaatat acgagcagta gataattctt tgcttatcag
    21541 cttactttga aactggttga aatgtacgac acgctgaggc gaagtttgat agaaatattc
    21601 gcaaaaacat cctgccaaca caaaaacaag cagaaatcca aaaaacgata ttttaccgct
    21661 ttgcctcata aaaaattttt tctctcaaga aatcatttgt ccttttttca tttcaagcat
    21721 gatgataggg ttcacccttt aaaatagtca tggctctgta gagttgctcc acaaaaataa
    21781 gtcttaccat ttgatgcgaa aaagtcattc gtgaaagcga aaccatattg tttgccctgc
    21841 gatacaagtc ttcagaaaag ccataggctc ctccagtaac aaaaactatg cgcttattgg
    21901 aagaatgcat cttttttgat aaaaaatctg caaattctac ggaagaaaat tccgtgcccc
    21961 tctcatccaa caaaatcaca tcatcgccga tttcgagctt ttttagaatt aaatccgatt
    22021 ctttggccgc ctgttcggaa gaagacaaat tttttgtatt ttttaattcg ggaatattta
    22081 tcacttcaaa attcaaataa tgtttcaatc gattttggta ttgttcttgc agtgaagaaa
    22141 ggtgaatatc aagagttttt ccaatattaa tcagaacaaa tttcatcaga atataattta
    22201 ttttttaaag gtctgatgga acccacatgt ctgacgtgtt tgtgtatata atacatgtgg
    22261 gtttcatata aaaagtttta ccgatcaaaa gcaatctaaa tcaaggaaaa gcggaaaaaa
    22321 cacgaaacgc tgaaaaaaat gtaattttgc aaaaaatatt tgataatttt gtccaattaa
    22381 tttttcaagc catgttacac aaattttctg ctgcaaatat gagacttttt taaggaattt
    22441 ccacttaaaa accaaagaaa aatgatcaca aaaaaaagaa ttgcgaattt taaatggcta
    22501 tcgatgattt cactgctgat gttcatcagc attcagcatt ctgcgcaagc acaaacttcg
    22561 agtattccaa acgaaattgt atcggcattg agcaatgggg atgccaatcg tttgtcggct
    22621 tatctgaatg acaatgtcga actctttatc gaaaacaaaa atgatattta cagccgccaa
    22681 caagctgtta gcattctcaa tgattttttt catcgtttca aagtttccga cttcaaagtc
    22741 atccatcagg gaaacaggga taattcgagt tttattatcg gtattctttc cacagctacc
    22801 gaaaatttca gggtttatat actgacccgc aaatcggata acaaaactat tattttacaa
    22861 ctcagaatag aaacatcaaa tgaatgaaga ttacaaacaa ctgattccct tatggtttgc
    22921 tgaagatata ggagacggtg atcacactac gctggcatgt attcccgaaa cggcgctggg
    22981 aaaatctcaa ctgatcatca aagaaaacgg tgtaattgcc ggcgtggaag tggcacgaga
    23041 aatttttaag actttcgatc caacattgaa aatgaatgta tttattactg atggaacaga
    23101 agttaaaatc ggcgatattg ctttcaatgt ggaaggaaaa attcagtctt tgctgcaaac
    23161 cgaacgcctg atgctgaacg tcatgcagcg catgagcggc attgcaacgc gtaccagaca
    23221 gtatgtcaaa gctttggaag gaaccaaaac ccgtgttctc gatacccgaa aaacgactcc
    23281 cggacttcga tttctcgaaa aagaagctgt aagaattggc ggcggcgtca atcaccgttt
    23341 tggtttgtac gatatgattt tgctgaaaga caatcacata gattttgccg gtggaatcaa
    23401 agaagccatt ttacgtacca aagaatattt gaaaaacaag ggaaaaaatc taaaaattga
    23461 aattgaggta cgcaattttg acgaaattga acaggtgatg gcaattggcg gcgtggaccg
    23521 aatcatgctc gacaatttca atattgaaaa taccaaaaaa gctgtcgaaa taatcggagg
    23581 ccgctatgaa atcgaatctt ccggcggcat cacttttgac acacttcgtc aatatgccga
    23641 atgtggcgtt gattttattt cggtaggagc gcttactcat tccgtaaaaa gtctggatat
    23701 gagtttcaaa gcggatattc ccaatcttat cgaagggatt cataaaattt tttaatcaac
    23761 tcgaattaca tttaattttt taaatcgata aatggagatt attgaaacgc ctattcccga
    23821 tttgttaatt attcggccaa aggtttttga agatgcacgt ggctattttt acgaaacgta
    23881 caatgaaaaa agttttatgg aagccggtct tgatattcat ttttgtcagg acaatcaatc
    23941 caagtcggtt tatggggtta ttcgcggatt acattatcaa ttatcaccct gcagtcagtg
    24001 caagcttgtt tctgccattc agggaacggt ttgggatgtg gcagtggatt tgagagcttc
    24061 ttcccctacc ttcggaaaat ggtttggtat tgaactttct gctgaaaatc atttgcaatt
    24121 tcttattccc aaaggttttg ctcacggttt ttccgtacta accgaaacag ctatttttgc
    24181 ctacaaatgc gatgcatttt acaacccttc gctcgaaaga ggaattcttt acaacgatcc
    24241 acagctggct atagactgga aaattcctga agataaggca attgtttcgg ctaaagatgc
    24301 taaacatccg atttttagca aagctgaaaa gaatttttaa gttttgtttt taaataaatg
    24361 tttttgaaca aataaataaa tcatgcaaac tattctcatc accggcagta agggacaatt
    24421 gggcaacgaa cttcaaaaag ctgcaaaacg ctttccaaac tttcgctata tttttaccga
    24481 tgttgatgag ctggatattt gcaacaaatc tgcccttgat aattttgtaa aggaaaaccg
    24541 gattgaaata attgttaatt gcgcagccta tacagccgta gataaggctg aagaggaagt
    24601 tgaaaaatgt tttgccatta atgcgcaagc cgtaaaaaat attggagaaa ttgctgaaac
    24661 ttatcagata aaagttgtac atatttctac cgattacgtt tttgacggaa aatcttacaa
    24721 accttatacc gaagggatgg aggtttctcc cgatactgtt tacggaaaat caaaactgca
    24781 aggcgaaaaa tttttgcaat cagtttgtaa tcaatgtatt gttatcagaa cctcgtggct
    24841 ctattcctcc tatggaaata attttgttaa aaccatgctg cggctcggaa aagaaagaaa
    24901 ttccgtaaaa gtgatttttg accaaattgg aacgccaact tatgcagccg acttggcaga
    24961 aatcattttg aaaattcttt cacaaaacaa ttttatttca ggcatttatc atttttccaa
    25021 tgaaggggtt tgttcgtggt acgattttgc tactgccatt ttcaaaattg cccatgttaa
    25081 ttgtttggtt catcccatcg aaacaaaaga ttatccggcc ccagcgccgc gtccctatta
    25141 cagcgtgctg aataaatcca aaattaaaaa cacgtttgga ctatcaattg cccattggga
    25201 ggaaagtttg gaaaaatgta tcgaaatatt aaaatctgag tattcattat aatttttcca
    25261 tcctcatttt tcgtacaaca aataagtttt tcatgctttg cgcatgaatt attttttgcc
    25321 gaaaaaaaca tggaaagtat taaaacatcg gctgtttttt catatttttg catatcaaaa
    25381 aattaagaac aacttttact caaacaagca aaaaatatat ttacgcatgg gaagagcatt
    25441 tgaatacaga aaagcaacaa aaatgaagcg ttggggaaat atggcccgcg tatttacaaa
    25501 attgggaaaa cagattacca tagctgctcg tgaaggcggc cccgatccgg atatgaaccc
    25561 tcgcttgcgc gtattgtttc agcaagccaa aaaggaaaat atgcccaaag aaaatgttga
    25621 aagagctatt aaaaaagctg tttctaaaga ggaggccgac aattataagg aagttatata
    25681 tgaaggttat gccccacacg gaattgcggt aattatagaa accgccactg acaatcccac
    25741 acgaacagtt gcaagtattc gcagctattt caatcgttat ggcggttcat tggggacttc
    25801 gggttcagtt caatttttgt tcgatcacaa atgtgtattt agaattgcac ccaagtccaa
    25861 tctttctatc gacgatctgg agttggaatt gatcgactat ggtgtggacg aggttattca
    25921 ggatgacgaa gaaaacaata ttgtcctata tggagatttt caacattatt cttccattca
    25981 aaaatatctg gaagaaaaca attttgagat attcagtgcc gaatttgaat ggattcccaa
    26041 agaaacaaag gaactgaatg atgaacaaaa agctcaggta ctgaaactga tagaaaaaat
    26101 tgaagacgac gaagatgttc aaaatgtttt tcacaacatg gcggaataat taatttcaaa
    26161 ttttacaatc gaatcagttt gattttttcc ttttaataaa aatacatatc tattcgacca
    26221 ttaaaaattc aattaactct tcaattaata cttaaaaaat ggatataagt gttttgttcc
    26281 ctgcattggc atccttaaca tggcaacata ttttgatgat tctcattgga ctaactctca
    26341 tatatttagc tatttttaaa gaatatgaac ccactttact tttacctatt ggttttggaa
    26401 ccattctggt aaatattcca ctgagttcgg ctattaatca aatagatggc aaaactggag
    26461 aaattattga aggtattttg tctgtttttt atgaaggagg aattgctacc gaaatgtttc
    26521 ctttgctgat ttttattgca attggggcta tgattgattt tacgcctttg ttcaacaatc
    26581 cgcatttgtt gcttttcgga gcagcagcac aatttggtat ttttatgacc atgttcatag
    26641 ccaccttatt cggattcgac ttgaaagaag ctgcttccat aggaattatt ggcgctgccg
    26701 acggaccaac ttccattttt gtcgcttcgc gttttgcacc tcatcttttg ggccctattt
    26761 ccgtcgcggc atactcgtat atggcgttgg ttcccattat tcagcctccg gttatcaaat
    26821 tgctgacgtc aaaaaaagaa agactcatca ggatgcagta cagttccaaa aaagtttccc
    26881 ggaaggcatt gatcgctttt cccattgttg taacaatcat ttcaggctta attgcccctg
    26941 cttctctttc attgatagga tttttaatgt ttggaaatct gattcgggaa tgtggcgtac
    27001 tcaacaaatt gtcgcaatcg gctcagaatg aactggcaag tttggtaacc atattattgg
    27061 gtatcaccat agccagtacc atgacggctg aacgtttcgt aacatttgga accctcacca
    27121 tcattgcttt gggattgctg gcttttgttt tcgatacttt tggtggagta atattcgcca
    27181 aacttttaaa cctgattttg ccggctaaga agaaaatgaa tccaatgatt ggggcttgtg
    27241 gaatttctgc tttcccaatg tctgcaagag taattcatac aatgggtttg aaggaagatc
    27301 cacacaattt cctgctgatg cctgctgtca gtgcaaatgt tggcggtcaa attgggtcgg
    27361 tagtagcagg tggacttata cttactttgg taaatttatt cgcttaaaat attgaaccta
    27421 tgatcaataa tgttgaagct gctctcgaaa taggagcaat cggtattaca ggcatattca
    27481 tttttatgct tatattttat gccgctatca aacttatttt gaaacttttc cctgaaaagg
    27541 aaaacaaaga agaataggaa ggagttaaat cgatttttga ttttttcgac aaccagatat
    27601 ttagtaaaaa cgcattttta agagttattt ttaactgctt aaaatgcgtt tttttcaatg
    27661 gaataaatta gtaaaggact aatattgttc tttttcgttg ggaaaatcgg aagattttac
    27721 atccgcaatg taatgttgaa tagcctgatt catttgttct cttaaattgg catatcttct
    27781 caagaaacgt ggagaaaagc cctgcgtaag tcccagcata tcgtgaatga ccaacacttg
    27841 cccatcaact ccacctccgg cgcctattcc aataaccgga atagagagtt cggaagcaat
    27901 ttgagttccc aactttgcgg gaattttttc caacaccaaa gcaaaacaac cggcatcctc
    27961 cagcaaatgc gcatcccgta taagtttttc ggcttcttct tcttcctgtg ccctaacatt
    28021 gtatgaacca tatttgttga tggactgtgg cattaatccc aaatgcccca taatgggaat
    28081 tccggcacca ataattcttt ttaccgattc aatgatttct tcgccacctt ccatctttat
    28141 tccgtcagca tgagtttctt tcataatacg aatggctgaa gcaattgctt cgtgagaatt
    28201 tccctgataa ctgccaaaag gcatatctac gacggcaaga gctcttttaa ttccgcgtat
    28261 ggccgatttt gccagaaata tcatctgatc gagcgttatg ggcaaagttg tggtatttcc
    28321 gcaaataaca tttgaagccg agtctcccac taaaattaca tctattcctg cttcatccac
    28381 aatattggcc attgtgtaat cgtaagctgt aagcatactg attttttcac cccgctgttt
    28441 catttcgaat aaccgacgcg tggttacttt tctgttgtct tcattgtgta ctgacatgat
    28501 aatttgattt gaatttgctg caaagttaca tcaaaatttg gttttctgtc tttttatttt
    28561 attgctgaaa ggaaaagaaa ccgatgataa aaatttcaac gaaaaacaat tttttttacc
    28621 tttgcattca aacaatttta gtgaaacgat atgttctgtg attttgagtt ttgttttagc
    28681 tttgaagtgt ttcaaaaaaa attactggat aaatgagtaa aattaccatt cacagttacc
    28741 aagaactgga agaatatgtt gggaaagaat tgggggtatc ggattattta accatcacac
    28801 aggaacaaat caataaattt gccgatgcca cactggatta tcaatggatt catgtgaatg
    28861 ccgaaaaagc aaaacaggaa agtccttata aaaacactat tgcgcatgga tatttgaatc
    28921 tttccatatt accttatctt tggggtcaga ttatggaaat ccgaaattct aaaatgacgc
    28981 tcaattacgg aattgagaat gctcgttttc aacaggcagt ggtagtaaac gatcaggtga
    29041 gagccaaagc aaaattaaaa tcgctggtca atttgaaagg aatagccaaa gcggaaattt
    29101 acactgttct tgaaattaaa aacagcaata aaccggcttt ggaaggaact ttcatttttt
    29161 tatatcattt tgaataactc atatatcaga gaaacgcaag aattaaaatt tgtgccgata
    29221 caactcttag aaacatggtt aaaggataca ccgtagaata agccacagcg gggatatccg
    29281 aaccggcaat tgaattggaa taggcaagtg ctggtggatc ggtcgtacta cctgcaagaa
    29341 gtcctatcaa agtaaaataa tttatcttta agaatttccg gccaattatg cccacaataa
    29401 gcagtggaag caaagctatg gctgctgcac atcccatcca aagcaaacca ttggcagaaa
    29461 taaccgtttc gacaaatttt tctcctgctc ctatacccac actggcaaga aacaaagaaa
    29521 ttcctatttc acgcaacata taattggcac ttgtagtggt gtaggttatt aatttcaact
    29581 tgtacccaaa acgtcctaac aaaatagcca caatcaaagg gccacctgcc aatccgagtt
    29641 taaccgaaac cggtatgcct ggaacaaaaa atggaatact ccccactaaa acacctaata
    29701 aaattccgac aaaaatagtt atgataggtg gttcgttcaa tttttgcaag gtattgccca
    29761 ataatttttc cactttttca atggcgtcca attctccaac taccattact ctgtctccta
    29821 cctgtaatac caaatcgggt gttcccaaca aatcaacacc tgaacgattt acacgggtaa
    29881 tatttacacc gtaaattgta cgtaatttca gcgagccgat agtcttcccg ttgatttttc
    29941 cgtgcgtaat cacaattctt ctggaaacca gtttttcttc ggatattttc caatccattt
    30001 ccactttttc tccaatatag gatgttatgc gttcaatatc tcgcgaagaa gcaactatca
    30061 gcaaaatatc gtctttgtat aacatggaat cgccttgagg aataaaaaat tctccattcc
    30121 gcattaaacg cgaaacaata aatggccttc caacagcccg tttcacatcc gtaactgtat
    30181 gattaaaaac cttttcattt tttacctgta cagaaatttt ttcgacctgt tcttcgtcca
    30241 cttcactggc tttttttatt tgttcggttt ccttgtgaaa atccacttta aaaattattc
    30301 gcagtaatac cattgaaaaa ataattccca atactcctat cgggtaagct accgcataac
    30361 ccaaagcaat tggcggaatt tctgcacccg gtgaagatcc aaaaacatgc cgtaatgctt
    30421 cctgagcggc tcccaagccc ggagtattgg taaccgaacc ggaaagtatg ccgaccagca
    30481 tgggcatgga cactttacca tgaagaagat aatatagggc tatcactatt cctacaccca
    30541 gcagcacaat catggtcgac aataaattga gcagcatccc accttttttg aaagaagaaa
    30601 aaaaagatgg tcctacttga aggccaatgg aataaacaaa caaaatcaaa ccgaaatttt
    30661 taataaactc caaaacttcc ggattcacgc ttaatcccaa atgacctaca aataaaccta
    30721 caaaaaggac aaaagtaact cccaacgaaa tcccaaaaat tttaattttt cccaaaagga
    30781 tacccaaaga aatgacaaaa gcaaacaaca aaatggtatg tgcaatacca tcattcaaca
    30841 aaagcgaaag gaaccactcc ataaaattat taaaaaattg attttcaaac aaataaatta
    30901 aaattgtcct tattgaaaaa tgaaagcaaa tttaaacaat tttcagcatt ggataaaata
    30961 tatattcaat tgcttctaaa tttgatcgaa cacttcaata tacttaaaat gaaacagata
    31021 aacaaataat tgtagcattc gttttttatt cagacaaaaa aaattacttt tgcattaaaa
    31081 atagtggtac atttcagaaa taaggtacat tacaagcata tcaataaaat aaacatggaa
    31141 gaaaatactg gtaaaaccgt aaaaaaaaga gtgcttttta tcgatcggga cggtaccatt
    31201 atagttgaac cttctgaaac ttttcaagtt gacagcttgg aacagctcga atttttgccc
    31261 ggcgttatcc gcaatttata ttttattcgt cagagaaccg attttgagtg ggcaatggtg
    31321 tccaatcagg atggtttggg gacttcggct tatccggaag aaaatttcga aaaagttcaa
    31381 tccaaaatgt tgaaaattct cgaaaacgaa ggaataattt tcgacaaaat ttttattgac
    31441 aaatctctgc ctgaagagaa attgccaacg cgtaaacccg gaactgccat gttgaaggaa
    31501 tatttttcgg caaattatga cttggaaaat tcgtatgtgg ttggcgacag aataaccgat
    31561 gtggaattgg caaaaaatct cggctgcaaa gcaattcttc tcaatgaaga tcctgaaatt
    31621 ctgcaggaat cgaagttgaa aaaagtctgc gtgttgcaaa caacctcttg ggacgaaata
    31681 gctgcattta tctttgcagg ggaaagaacc gccaccatac aacgaactac gaaagaaacc
    31741 gatattttta ttcacctgaa tttggatggc tcaggaaaag gaaatatttc taccggtttg
    31801 cgttttttcg accacatgct cgaacaaatt gcccgtcatt cgggttgcga cttgaccatt
    31861 caagtgaaag gagatttgga agttgacgaa catcatacca tcgaagatac cgctattgct
    31921 ttgggagaag cttttgccaa agcattggga gacaagcgcg gaatagaacg ctatggattc
    31981 tgcttgccaa tggatgatag tctttgtttg gttgccatcg attttggagg acgagcatgg
    32041 ctgactttcg atgccatttt taaaagggaa tatatcggcg attttcctac cgaaatgatt
    32101 tttcactttt tcaagtcgct gagcgatgcg gcaaaaatga atctttacgt aaaagcactt
    32161 ggcgaaaatg aacatcacaa aatagaagga attttcaaag cattggccaa agctattaaa
    32221 atggctgtaa aacgagatat ttaccaattt gaacttccta caaccaaagg aattatctaa
    32281 tttttaactt atcgacctat cattattgat catggatttt aaaagaaatt ctgcaaaatt
    32341 tttctttttg atttttttac ttatcgttca atttccactc cacgcacaaa tttttcctga
    32401 ccagccgcgt ttggtaattg gtattgtggt ggacggatta caggaaaaac acatcgattt
    32461 gctttggcaa cgttttgatg caaacggttt taaaaaaatc attgaaaaag gtactgcctt
    32521 tgaaaatatg gaatataaca ttgtgtcggc cggaaatgct tcggatattg caaccctgat
    32581 gaccggcact acaccttatt atcacggagt tacaggaaat aatttctatt ccagaaaaca
    32641 gggaaatgtt caatctgtga ttttcgacga aaacgaaccg ggcataggaa caaaacaaac
    32701 cgtttctgcc catcaacttt tggccagtac cattacggat gaattgatgc tgacctatcc
    32761 gaaacaatca aaaacttatg ctatcgccat caatgctgaa gatgccatta tgttgggtgg
    32821 acatacagcc aacagcgcta tgtggctcga cgacatcaac atgaaatggg taaccacttc
    32881 gtattatacc gatggtttgg ctgccagcgc cgatgaaatg aatgtgaacg gacaaatcga
    32941 acaatatgct tcgtccgttt gggaaccgat gtactcgccc aatacttatt tgtttccttc
    33001 agcgaacgaa aacaaaaaaa acggatttca gtattttccc aacagtaaaa gaaaaggatc
    33061 caacctgccc attttaaaaa atacgcccaa tgccaatgct ttggttgccg atttggcttt
    33121 aaaaattttt gaaaaggaaa atctgggaca ggatacttat cccgatatgc tgctcttgca
    33181 atttaacgta aaaactccct atgaaaaaac atttgcgcta aaaactgccg aaaaggaaga
    33241 tatgtatttg cgcttggaca aagatttaca atatttattt caaaaaatcg acagcaaaat
    33301 aggcttgtcc aacacgttaa ttttcattct gggtacccaa acctctgctc atacaccttt
    33361 agaattgggc gaaaataaaa ttcctgcagg atattttaac ggacgtcgat cgatggcttt
    33421 gctcaacact tatttaatgg tgctttacgg acaggaacgt tgggtggaag gttattacgg
    33481 aaaaaacatt tttttgaatc gacagaaaat cgaagaaaaa aaaatcaatt tgcaggaaat
    33541 tcagcaggta gttgccgatt tcatgcacga gtttgaagga atccagtcgg cttacacttc
    33601 ttatcagatt gttaatctta ctgatccatc caacagggaa atgtcgaaac tgcgcaattc
    33661 atttcaaaaa agcagcaagg gagatgtgat cattactttg cttcccggct ggctcgagct
    33721 cgacgacaac aacaatccca taggtgagtc gaatgcggtt atttcttcaa caccagttta
    33781 tttctacgga tggaacatcg aaccaaaaaa aattgcagca atccattcga ttattgacat
    33841 agctcctacc atttgcaaaa ttttgaaact tccttttccc aacgcaacta ccggaaaacc
    33901 tttggaagaa attatcaaca agtaaaaatt tttttagcag ataatgaaaa aatacgattt
    33961 cgacgaaatt atcgacagaa cgaacaccaa tgccgttaaa atagaagctt gtcggtcaat
    34021 gtttggaacg tcgaacgtta ttcctttgtg ggtagccgat atggattttc gtactcccga
    34081 tttcattttg gatagtatcc gtaaacgctg tgaacatccc attttgggat attctgttcc
    34141 tcccaaagat tattttccat ccattatgaa ttggctgaaa gaaaatcatg gctgggaagt
    34201 taaaaaagaa tggctgggat tcatgccggg tgtggtgccg ggactttctt ttgccgttca
    34261 gcaattttct gccgaaaatg atgaaattgt tgttcagcca ccggtatatt ttccttttat
    34321 gcatgtggta gaaaaaaaca acagaaaatt gatattcaat cctttaaaag aggttgacgg
    34381 aaaatttcag atgaattttg acgatttgga gaaaaaaatt acttcaaaaa caaaaatgtt
    34441 gattttgtgt aatcctcaca atcccggagg aagaagttgg ggaaaagaaa ccttgcaaca
    34501 attggctgaa atttgtgcca aacatcatct gatagttgtg tcggatgaaa ttcatgccga
    34561 tatgacgttg cccggaaatg tgcatgtgcc ctttgcttcg gtatcggaac aagcagcaaa
    34621 tatcagtttg acttacatgt cgcccagcaa aacattcaat atcccagcgc tgcaaacttc
    34681 ctatttcatt attcccaatg aggagcttcg aaatggactg aaaaattttc tcgatcgttc
    34741 ggaactccac aacggaaata tttttgctta cctctctacc attgccgcct acacggaagg
    34801 aaaatggtgg aaaatgcaaa tgcttgatta tgttcaggga aatatcgatt ttgtcattca
    34861 atttttgcag aatgaaattc cacaaatcaa acccatgaaa cctgaagctt cctttttaat
    34921 ttggctcaac tgctcagatt tgggtatgaa tgaagaagaa ttattccaat tttttgtaca
    34981 caaagccgga ttgggtttga atcagggcat aactttcgga cccggaggtg aacagcatct
    35041 acgactgaat gtggcttgcc ctcgaagtac cttagaaaaa gcaatgaatc aattgaaaaa
    35101 cgcagttaga aatttataaa tcaatatcta tgtggggaac tctggtaaat gcactggccg
    35161 tgattgtggg tggaagtgcc ggcttgttgc taaataaaaa aatgcctcaa cgtatccaaa
    35221 ccatttattt tcaagtaatt ggacttttta ccataggaat cggtattaca atggtagtcg
    35281 gtatgaaaca tattttgctg attgttatga gtttggtgat tggtgttttg attggggaat
    35341 ggatgcattt ggaaagtaga ctggaaaaat ggagtgaatc actgaaaaag cgttttcaaa
    35401 ttggcagtga gcaattttcg gaagggtttg ttacggcatt tttattgttt tgcattggat
    35461 ccatgactat tttgggatct attcaggaag gactgaccgg ttcgccagat ttgctgttca
    35521 ccaaatcgct aatggatgga ttttctgcca tcattctggc ttcggctttt ggtgtcggag
    35581 taattttttc ttccattccg ctccttattt ttcaaggtgg acttacactg ctggctatgg
    35641 ctgcatcttc tttttttact cccgatttga tcaacggaat ttccaacatt ggcggaattt
    35701 tactgattgg tttaggaatc aatatactcg aaattaaaaa acttgccatc accaacatgc
    35761 tcccttcact cgttgtagtt atccttttaa tatggggttt tgcataaaat tcgttgtaaa
    35821 ttaaaaattt cgctcctgaa tttcactttc aatgaagaaa aaagcccgag aaaacccggg
    35881 cttaattttt tactacaaat aaaaaacata atttcggaaa aaggctatgg tcgctgctat
    35941 gataaccaaa atcattgaaa gcaaaatggc tgcaataatc agaacagccc aaataaccag
    36001 ctgcatagca ttggaaccgg tgtcgtattc ggttgtttta ctaccatcgc tccatgtttt
    36061 ttcggtgtaa taatctactg ccactgcgga tgctgctgct cccatgataa aagcaataat
    36121 tttgccgaaa actccacccg aattatgttt tcgatttgca accacataat tgggtgcaaa
    36181 attggaaagt atataagcta cggtcggtaa aaacatcacc atcgctccta ttgtgccttt
    36241 ggtatcgaga tgattttttg caagcagaaa atataccacc ggaaaaatca gcgataaaac
    36301 tgccaaaaac cagtttccat aaaattttcg tttgctgagt ttgttgagca agcctcccaa
    36361 atcattcata cggtcaatta cttcattatc ggtcggttga taagattttg ccttgtttaa
    36421 agcacgatag gtagttttaa aatgttttcg tttggttggc gaacggaaaa tctgtccgtt
    36481 ttcttcactg acgggttccc aagtatcaaa agcttcttcc acacattccg aagccaaccg
    36541 attggcatcc tccttttcac tgtcgatacg ggcatttttg ctgtcgccac tgaaggaagt
    36601 attatttgca gtttttatcg gtttgtatct tttttcggtt ataagtacca caatcatata
    36661 aatgaccgaa acgaaaagga aaataatcaa tccggcaagc aaaactttct gaatagtttc
    36721 atttcgttcc attctctttt gtgcatcagc aaaggaaatt ccactaatcg acgacaactc
    36781 atcggaagaa agcgccttgt tgtaaacccg aatttcgtcc acctgtcctt taaaagcccg
    36841 gttgtatggc gaattttgcg tgtttccaat aatcaggaaa ttgctttttt ttgtaaaaac
    36901 taccggtttc gattcgtagt attcgtcatt gacataaagc cgtaaggtag aatcatttgc
    36961 gttggccgtc atggcaataa aattccattt gtctttcttt aatgtcgcac ttcgaagcga
    37021 tgcaaaataa ggatttttca gagaatccaa tttattgaat ccacatatta cggcaccttt
    37081 agaaatttga agcgatcgtg aactgaattt tttttcatta tttaccggaa gtgtcgagcc
    37141 aaaaaggaat ccatctggac gtccgaaaac ccatgccgta taggtaattt cgggaaggtt
    37201 ttgaggtgaa atatccagat gtgttttaag tccttgagca tttccatcaa ataccacagc
    37261 cgtgctgtct ttaccatcct tgccagtcgt ccatttgtaa tagtaattgc cgggttggct
    37321 tttcaatttt aagtgattat tatcggaaac cgaatttttg ccgtttccat tcaattcgta
    37381 aaaaagaatc agatgagacg acagcgtgtc ctgattctgg gaaaaaattg cctgcatgca
    37441 aaaaagcatg agcgtcagta aaacagtgat tttcgttttc atggtttaaa tttaattgtt
    37501 aaaaaaatgt ttgcattttg cctgtaaaca aacaaaaaaa caatttatct acacgcagtt
    37561 tcttatttga attgattttt catactgact tacaaaaaca acactaattt tcaaattctg
    37621 tcgaaatgcc ctctcacaat caagaaaatt ttcaaaaagc tgtcatatca aaattcgatt
    37681 acctcttttt cagcttcaga ataaaaagta tctatttcct gttgaatttc aatggtataa
    37741 ttcaatgccg aaattatttg cgaataatga acaaaatcat ccatttttct tccctttctg
    37801 tctttcagat atttttgaag tacccgataa cctccaacct gatagtccca aacctgaggt
    37861 gttacattat cgaaatattt ttccgaattg atgtaaatcc gactttcttc cgggcgaaaa
    37921 attatttctt ctataacgtc attttcgcct tcgccctgaa atttggaaac aagtgtattg
    37981 agtcgactgc ttttcatcaa atgtaaatcc accaattcat ttcccaaagc agccattttg
    38041 ctgaaaacct tgtaatcagt tgtgaaaggc acacgtggaa aatcgatttt caaaaactcg
    38101 gcatattttt cacgataaat attgctgtaa aagacggcat aaatgtaata cagaatttct
    38161 tcgggcgtaa gttcatgccc gtagttttgg ttcaatgttt gataaatctt tttgtcgata
    38221 ttgggctctt tgttgctatt cccgtattcg gtttccggtt caaacaacat catcatgtat
    38281 tgcgtgcggg atttttttgg agtatgctct ttgtaaagat aaaggggaaa agcataacaa
    38341 atttcgcttg ttttatttga tatatagcaa ctttcgatta acttatcgga aactaaacta
    38401 tgttgccatg tttctccggc ctttacttgt cttactgtcg ttattgaaag attttctttc
    38461 agtatatgag acataatctg cttcacagtt ctccaaacca atgaatcgtg ccaaaaaata
    38521 tatcgatgat caaatggtcg gtaaataaaa tcaaaaataa aattttccaa ctcattatca
    38581 ggaatatctt gtaacatatt ccatgcttta cgaatgttcc aacctatttt tttgttaata
    38641 tgaaaaaatt gatagagttc gttatcacta aattttgagt ttttaaaaag tcttattctg
    38701 ttcaaaagag cttcttttct gaaatctata acgaaattat ctctggcagt tgttatgcca
    38761 acaccattta caggaaaaat ttcgtttatt tgcttccatt tcaaataata tttgatgttt
    38821 tgcgtatttc tcggaataaa gaaataccac ggactttctg gtttcagcaa ctgataattt
    38881 ttaatttcca attgatgggc atccagccag tcgtatttac tttgccgttt gccaaaaagt
    38941 tcggcatgat aaaccttgca gtcggatttc tccttttgtt ttacaaaaat ggctattgcc
    39001 gttccctgac gaatatcaaa aacattttca tcttttcctc catccggcgc cgtttctttt
    39061 ttcagactgt tgccatgcaa gtcgagaatg tatatttcat tgaaagtttt catcaaactt
    39121 tgcctcattc cgcgaaaagt cggattatcg agataactgt ggttggtaat catacccact
    39181 acaccaaaac cggctttttg aattttccac tgagcgaaac gcaaaaattt gacataatcg
    39241 tcctgtaacc atttcggatt tttctctccg agcgttttgc cgtccacttc ataatagctt
    39301 tgagctccat caatattttg tttaagcagt ttttccgtcc attcgttaat attggccgaa
    39361 attccgctgt aaggaggatt tccaagaatt accagaacgg gctgttcctt tttaattttg
    39421 cctgccaaat gactttcttc actcaacgaa ctgaggccgg gtatggaaat ttgctccagc
    39481 tcatccattt ccaacgtatt ggtcaaataa agtttaaacc tttcctcatc attgagtttg
    39541 taacccagct cttccagcaa aaaactcatt tttaaatgtc caatggcgta aggcgccatc
    39601 attagttcaa aagcaaaaaa gttctccaaa acctgattac ggataaaatt gtttcgtcca
    39661 ccactcccat atttttcggt aaattctttt acagccaact ttacggcttc agcagggaaa
    39721 gtaagcgttc ctgctgccgg atcaagcaag gtaacatttt tggaagccag tccgtcaggc
    39781 atatcgaaat gcgttttcaa aagttcgttc accgaccgca caatatatct aacaacaggt
    39841 tcaggcgtat agtaaacacc tcgtttttca cggatggaag gatcataggc tgtcaaaaaa
    39901 gtttcgtaaa aatgaacaat tggatcttgc ccctttcctt cttcaaaata atcttccaga
    39961 attttcctta catccgtaac gctcaaaact tcagcaatat cgtctacaat gacttgtaaa
    40021 ggtttgggtg gatcttccag tgaaataaat cgaaaaacgt cacgtagaat gccaatggta
    40081 ttgggaatat atttgaaagc gagctcgcga ttgaaggaat tatcagaacg cgtacgggca
    40141 gcaaacaatc cgtacgtcaa tgtttgagaa tacaaatcgg caaaagcttc ttttgtcaaa
    40201 gttccaatca gatatttacg aaaagcttca taaaaaccaa gaatagcttt tttaccacgt
    40261 ttttcttctt catcgagttc gatacttacc acttcatcac gcaagaatcg ggtgcgtttt
    40321 gccaactctg ttgccagcga ttgagcattg cgtacgtgag gcagcgaaaa ggaaaaaaac
    40381 gtatcgagca aatcgaaaaa agccgtttcg ttttctaccg gaggaacagt ttttagcttt
    40441 cttgccaact caggtcgtcc aaccagaact tgttttatca actctccatt tcgataaagc
    40501 cgaaactcgt aaaaattggt aaggataaca ttcggaaaag tttcgcggta acgtttcaac
    40561 tggcctgaaa cttcgattct gtcaagattt gtaacagaag ggtctttggc ttcaatatag
    40621 ccggtaatat gatttttccc gtcccaaata cgaaaatcgg gattgccggc ttcggttggt
    40681 ttgggtaaaa cagtaatgtc aaccttattt gtctgacgtt catcagtata attcagcaac
    40741 aaatctttca aatgcccgta ataactctct tcacgtgcat caccttgatt aaaagtcgct
    40801 tgaaggctgt ttaaatactg tttcagcata aaataaataa catgggaaaa acacaattaa
    40861 aaaactcaat cactcaaaga gattggaaaa aaacatgtgt aaaagaaatg aaatatagcg
    40921 tgttgcttgt aaagacaaag aaatgaagag aaaattaggg tttcatgaac cactaaaata
    40981 aaaaactttt gaaaaaaaag cggaaagtga gagattcgaa ctctcggtac agttacccgt
    41041 acaacggttt tcgagaccgt cccgatcgac cactccggca actttccggt caacaaaatt
    41101 ttatgttgca aaaatacagt tttttttcta atattaaaga aaaatctcgg acaaaaccca
    41161 aaaaatcatt ttcataaaat ataaatactg tcgttttgat aggataattt taaatttaac
    41221 aacaatacaa ataaatttct tccgaaaaaa ataaaaatgt caacagtcat cataagaaaa
    41281 ataataaaaa atattgcaaa atttccgctt tttgatatac agatattact attttgttat
    41341 aactttgcaa ccgaaaacta taaaaaataa aaaacataaa caaaatgaaa gtagaacagg
    41401 taattgacaa tttgaaaagg agatttccca atgagccgga gtattatcag gcagtacagg
    41461 aagtattgga atctattgta gatgtttaca atgctcatcc cgaatttgaa aaacaaaatt
    41521 tggtagaacg cttggttatt ccggacagaa ttttatcttt ccgcgtaact tgggttgacg
    41581 acaagggcaa tattcaaacc aacatgggtt acagagtgca gcacaacaac gccattggac
    41641 cttacaaagg tggtttgcgt ttccatcctt ccgtatatcc cggaattttg aaatttcttg
    41701 cttttgaaca aacttttaaa aacgccttaa ctactctacc aatgggtgga gctaaaggtg
    41761 gttccgattt taatcccaaa ggaaaatcgg acgctgaagt tatgcgtttt tgccaggcat
    41821 ttatgcttga attgaacaaa attgtcggtc ctgataccga tgtacctgcc ggcgacatag
    41881 gcgttggcgg acgcgaaatc ggttatatgt acggcatgta caaaaaactc agaaatgaag
    41941 ccaatggtgt tcttacaggg aaaggtctcg aatttggagg ttcactgatt cgtcccgaag
    42001 ccaccggata cgggaacatc tacttcttga tgaatatgct gaaaacaaaa ggtattcacg
    42061 atctgaaagg tcaagtggtt gccatttccg gttcaggaaa cgttgcaacc tacactgccc
    42121 agaaagcact ccaactcgga gccaaagtta ttactttttc cgacagcaac ggttatattt
    42181 atgacccgga tggcatcgat gaagaaaaac tggcttacgt tttcgaactg aaaaatgttt
    42241 atcgtggacg tattaaagaa tatgccgaaa aatatggctg caaatatgtt gaaggtgcac
    42301 gtccctggag cgaaaaatgt actatcgctt tgccttcggc tacgcaaaac gaaataacag
    42361 gcgaagatgc cgaaatgctt gttaaaaatg gttgtattgc cgtttccgaa ggagccaata
    42421 tgccttcaac tcctgaagcc gttgatgtgt tcctgaaaaa taaaattctt tatgctccgg
    42481 gtaaagcggc caatgccggt ggagtttccg tatcaggatt ggaaatgact caaaattcca
    42541 tgagattgaa ttggacaaga gaagaagtgg atcaaaagct tcagcaaatt atgcaaaata
    42601 ttcacgaagc ttgtgtaaaa tacggaactg aagccgacgg atatgtaaat tatgtgaaag
    42661 gtgcaaacat tgccggattc atgaaagttg caaaagccat gatggctcag ggcatcattt
    42721 aagcaatttt tttaaaaaac aggaaaagag gttctccgat aattttcgag aacctttttt
    42781 tattctactc aaaggaaaaa caagctgacg cctgctatgc tgacaaatgc tccgattatc
    42841 tgtggaatgg tgattctttc tttaaacata actgccgaag gccaaatgat aaacaccgga
    42901 accagtgcca ttaatgtgga agctatgccg gtttttgtat gttgaatggc aaacatggat
    42961 aacgccacgc ctacaaacgg gccgaaaaat gtgcctaccg ttaccgattt taatgcggat
    43021 ttgtttgtaa aagcggctga gacttgcttc catcgaccca aaaaagtgat aaataaagca
    43081 aaacaaacta gtccaaacag ggctcgtatt tgagtagctg caacaggatc gtaatttcca
    43141 attccttttt tgcttaaaat taatcctacc gcctgtccca aagcacctcc aaaagccagt
    43201 aaaaaacctt taaaaggaat attcagcttc aaattccgat ttgaaatggc tatgataatc
    43261 cctgttacac atatcactat gccaagaata cttttcaacg aaagcttttc atccaaaaaa
    43321 aaccaaccga ttaaagccgt caacatggga cacaagctca taatcaactg tgctgtacgc
    43381 gaaccaatca ccatatagga tttgaaaagg aacaaatcgc ccaaaaagaa acctataata
    43441 cccgaaagtc caagccagaa ccattgataa tgtgtagcat cagtgggaaa gaaaaagccg
    43501 cgtgtaaaaa aagtagtgat acccagaaat accactgcca acgataaacg aatcacgttc
    43561 accgtaagtg aaccgatttt atgaccggct ttctcaaaaa acaaagcact cattgtccaa
    43621 cagagcgcta
//
LOCUS       DAOSCH010000005        43453 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_662_length_43453_cov_5.663671, whole genome shotgun sequence.
ACCESSION   DAOSCH010000005 DAOSCH010000000
VERSION     DAOSCH010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 43453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 43453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43453
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_662_length_43453_cov_5.663671"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..979
                     /locus_tag="PLZ54_00960"
     CDS             158..979
                     /locus_tag="PLZ54_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HOJ65543.1"
                     /translation="MSFEKYKTILHQIGDKTKTIRLNGRGESTIHPDFVEILNYTKQH
                     FPQLNINLFSNFSFNNKKIIDALIANGVQLFISMDSPKADELSAIRKGANFQFIENNI
                     KQLQELPNRPFVIFTIQEANINRIFEMAEFAFENNCQILYNTIRRDEGVEVFIEIVKQ
                     NYQSILEQFKQIGKLYANSELQFFYPDQLAGVELQTEKPTKTHGTMKQCPALEKELCI
                     LYDGTATPCNMFNPYTYGNIFNQSLSEIWESKERKIFLSSHKCHYYCQNCANLGV"
     gene            983..1942
                     /locus_tag="PLZ54_00965"
     CDS             983..1942
                     /locus_tag="PLZ54_00965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ65544.1"
                     /translation="MSYLSNVPYGKILYYWYEKLSHNPYRKKFLWTKIIEIIAFKRHS
                     SKQNNKIYQYVDFLQPIANDISENPIVSIVVPTFIRNQKDINDIENLLLSISKQTVKP
                     DNIIIVDDFSPISYSFPEHIQVYRQTKNSGPAKARNWAKKNAVENKSDIIAFTDTDCI
                     LSENWVETIIKSFQQSKDFQILSGNTISYDKNWFGIYHNINGTLNGRKFKDSERLLYG
                     TTANLSTTRQVAENIDFSEEFSLAAGEDIEFCFRANHDCFAIKHIPQMIVFHNYGYTN
                     GLWKNLKSFTRQFMRYGQGEVTLLQEIPEYYVYFDKTEEITAV"
     gene            1939..2877
                     /locus_tag="PLZ54_00970"
     CDS             1939..2877
                     /locus_tag="PLZ54_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ65545.1"
                     /translation="MKQILITGGAGFVGSHLCERLLNEGNKVICLDNFLTGNKQNINH
                     LIENPNFKIVEHDICEPYFTEIDEIYNLACPASPIHYQYDAIKTVKTSVLGTINVLGL
                     AKRTNAKILQASTSEIYGDPIAHPQNESYWGNVNPIGIRSCYDEGKRCAETLFMDYHR
                     QNNVRIKIARIFNTYGTHMQANDGRVVSNFIVEALQNKDITIYGNGQQTRSFQYVDDL
                     IDGLIRLMNTRDNFVSPVNIGNPTEFTILELAEKIIDLTNSKSKIKFFSAVQDDPKQR
                     CPDISLAKEVLNWQPKIDLESGLKKTIEYFENILTD"
     gene            3065..3799
                     /locus_tag="PLZ54_00975"
     CDS             3065..3799
                     /locus_tag="PLZ54_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65546.1"
                     /translation="MSKSYLIVFHGQIVNDKIVDIGDDPCFDCPPSWGICRPSTRKSV
                     KVGDTLIFIAKVDNDYFLKGWFKVGNKLDYPFALQQFPSRQNVIISKTISSHPIKWRY
                     KNLQKSYYKTHGQTTPNFLIQLDTGSGTFYQSQTDDHETDNWKCRRIFHCTSKQFEKC
                     IAANNCLKSSELLTANQYKNYVVADKNHWADLDNLRITFDEIKKATNFPKKIRTPKGQ
                     HNVMRFDDYKEKLFAFIEKRKNDAKK"
     gene            4041..4607
                     /locus_tag="PLZ54_00980"
     CDS             4041..4607
                     /locus_tag="PLZ54_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HOJ65547.1"
                     /translation="MACIWKKRLKRNFSKLQVGDKVIGYFSGKSKSFVSIGVIEKSLS
                     QDPNKKEIAIRKICDLQSPMSLGRVRQIKPFDNKLSNNGKLNTTVIPLTLDEYNLLMS
                     IIHGAFLDVYDDVDSDLNSVDMPIKEALKLRPAKQISNSTTWIRDSRISKAALKHANY
                     ICEFATKSDNHESFIANYTKKIMLKHTI"
     gene            5349..5666
                     /locus_tag="PLZ54_00985"
     CDS             5349..5666
                     /locus_tag="PLZ54_00985"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOJ65548.1"
                     /translation="MADVIKNAFLAGLGLVSLTREKAEEFAKDLIKRGELTETEKPKF
                     IKDLLEQSEKTKTEMEEKVEKAVDAILKKMNIPSRKEFEELKNKVEELTQLLNKKQEE
                     SEN"
     gene            5671..7362
                     /locus_tag="PLZ54_00990"
     CDS             5671..7362
                     /locus_tag="PLZ54_00990"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOJ65549.1"
                     /translation="MNDSYPYRNFGFRNNLDKVKRSRQIISVFVKFGLDYLFEASRVN
                     FLSKLRKRRKVYHKLSTAERLRLAFEELGPTFIKFGQILSTRPDFLPAEYIRELEKLQ
                     DQVLPMETPLVRRCIEKELKHTLEELFLDFDQSPVASASLSQVHKAVLQNGDTVALKI
                     QRPDIHKTIETDLSILESFAEILEKRLRNSSLYHPTAIVAELKKTIRKELDFMNEAHN
                     FEKFRKNFKNVDYIQVPKVYWQFTTEKLLTMEFIEGVKITEITQEKYEHRYEPKKVAD
                     RAAKALLKQILIDGFFHADPHPGNLFIQPPATIVMLDVGMTGHLDEESIITIARILRA
                     VNEKDSNHVIKGLQELGIVVKETDKRQLRHDLTELIEMYADRPFRTISLNRMNQQLLE
                     IMARHDMALPSNLVMMLRAMSIAESIGQQLEPDFDMFAIVEPFVKRIYLKLYSPKSFR
                     KRSMNVMDESIELIELFPQNISDLLGKLKEGQFEIKLEHQNLENLQDEMKSSSNRISA
                     SLIIAALIVGSSLILQQRIGPLISDISILGVIGYLLAGIMGLGLLLTIFKTRRKK"
     gene            complement(7417..9531)
                     /locus_tag="PLZ54_00995"
     CDS             complement(7417..9531)
                     /locus_tag="PLZ54_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOJ65550.1"
                     /translation="MKAVFILSFSIVFMAACHSFSKIAYPHTQKENVTDHYFGTDVAD
                     PYRWLENDTSAATAAWVKAQNEVTFKYLEKIPFRNALKNRLTQLNDYPKLGAPFKKHG
                     KYYFFKNNGLQPQSILYQQSTLYSEPEILLDPNTFSSDGTVALSSVSFSNVGNYMAYS
                     VAKSGSDWNEIFVLNLDNRQTLSDHIQWTKFWNVAWKDNGFYYIAYDAPQKGKELSAK
                     NEFPKVYYHQIGQLQSKDLLIYQDKNHPLREFGPFVTEDEKYLFLIITETTTGNALLM
                     KDLTDPKAKFVSLVDGFENDYSVIDHINGKIYILTNWKAPNKRIMEVDPAHPSRENWR
                     EIIPEQKTVLELVEIIDGKFFCLYLKDAAHRLYAYDLSGKLLYEVKLPALGSVVSLTG
                     NKDDSEAFFAFSSFTYPPTIYRLDAGKNVSEIFQKAQVKFKPEDFVTEQVFYTAKDGT
                     KIPMFLSYRKGIKKDGKNPLMLYGYGGFNISMTPSFSISRIPFLENGGIYAVANIRGG
                     GEYGESWHMAGTKMQKKNVFDDFIAAAEFLIAEGYTSSKKLAINGGSNGGLLVGACLT
                     QRPDLFAVAVPEVGVLDMLRYHLFTIGWSWASDYGTSEESREMFEYLKSYSPLHNVVK
                     GTKYPAVLIMTGDHDDRVVPAHSFKFAATLQEANAGNKPVLIRVDNNAGHGAGKPLGK
                     LIDAQTDMWSFVMYNLGMKPKW"
     gene            complement(9563..10837)
                     /locus_tag="PLZ54_01000"
     CDS             complement(9563..10837)
                     /locus_tag="PLZ54_01000"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOJ65551.1"
                     /translation="MKKIFYFIPLLLLSCATMAKNNGNPLKFKHSERSFAAFDTERLA
                     RLDTVFQDLVRKGEIPHAVTFVAHRGNIVHFKAFGWRDKEKNIPCQRDDIFRMASQTK
                     AITATGVLMLMEEGKILLDDPVKKYIPEFAHPQVLVSFNEKDSTYTTRPANKDITIRH
                     LLTHTSGISYGTDPNNPMMKIFAKKGIPPLNSLDPVTLKEVVKKLASCPLEHDPGERF
                     TYGMSVDVLGYLIEVVSKMPVDQYFKEKIFMPLGMNDTYFYVPKEKQNRMVTLYQKVQ
                     GKTLQPSSSVLYQTFPYSGAQTLFSTGAGLSGTIEDYAKFCQMILNKGEFNGKRILGR
                     KTVELMCRNQVGDLRGEIGFGLAFDDFRPQYLHLTMASEGSLRWGGMFGTDYLIDPKE
                     DLILLFYINWQTDNSVPDVKVLFHNLVYQALK"
     gene            complement(10851..12347)
                     /locus_tag="PLZ54_01005"
     CDS             complement(10851..12347)
                     /locus_tag="PLZ54_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ65552.1"
                     /translation="MNQEDTFPTTDSLKDFQQNFTKEPTIENLKNALNNEIFNIISDI
                     ADEAHQECYVIGGFVRDLLLRRPSKDIDILVVGNGIELAEKVVNQLKQLNKPCKFAIF
                     KNFGTAQIKLKEMDVEFVGARKESYRSDSRKPIVEDGTLQDDQNRRDFTINTLALCLN
                     KSRFGELVDPFNGLRDLQNKVIRTPLNPDITFSDDPLRMMRCIRFATQLNFFIETNTF
                     EAIGRNKKRIEIISKERITDELNKIILSPKPSIGFILLDKSGLLDFILPELSALKGVE
                     TKDGIGHKDIFYHTLMVLDNLSQTSDSLWLRWAALLHDIGKPRTKRFDKRQGWTFYNH
                     NIVGEKMTADIFRRMKLPTNEKMKYVQKMVSLHMRPIVLSEEEVTDSAVRRLLFDAGD
                     DIDDLMLLCEADITSKNPEKVKRYRENFQLVRKKLKEIEEKDRIRNFQPPIDGKEIME
                     TFHLPPCALVGEIKMKIKDAILDGIIPNEYEAARSYMMQIAAEMGLTN"
     gene            complement(12650..13909)
                     /locus_tag="PLZ54_01010"
     CDS             complement(12650..13909)
                     /locus_tag="PLZ54_01010"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOJ65553.1"
                     /translation="MKNILSTVVFIFLFVNSSLTAANPYRTKILKPNIKTLQIRISDT
                     DFGFPILKLNGTETLTVEFDEMSHNVRSFGYTVLHCNADCTVSNLNSNEYVDGFTRGN
                     IDDYAFSQNTTFQYIHYRFTIPNQDMRFKISGNYVVLIYEDNRVDDPVATACFSIVEP
                     KVEIDARVRANTDIELSGRYQQLDIEVNLANYVVQDPASEVKLVVRQNNRFDNEVANI
                     TPTFFNAGKLSFVNNKALIFEGGNEYRHFDISSFYAAGEGVDRIIFNRKYYEAFLKED
                     LMQKSRTYISQPDANGRFIINFQEAFQDVQTEADYVQVHFSLLSEQPFFDGQVFIGGD
                     YNYNLLDDTARMQYDFDAQRYTKTLLLKQGGYSYQYWFLPKGKNSASTGKIEGSFWQT
                     KNEYTIYFYHRAWGERYDRLIGYSVID"
     gene            complement(14695..15207)
                     /locus_tag="PLZ54_01015"
     CDS             complement(14695..15207)
                     /locus_tag="PLZ54_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOJ65554.1"
                     /translation="MLNDKITEMLNNQINKEFFSWYLYLDISNYYANKNLDGFANWFS
                     IQAQEEYAHAQLFLKYILNNGEKVKLLPIAAPESNFVDFKAPLSAAFEHELFITASIN
                     EIYAEAYAQKDFKTMQFLDWFVKEQGEEEKNVDDLCKRFDLFGTDPRGLYLLDNELAS
                     RVYTAPSLVI"
     gene            complement(15243..16553)
                     /gene="murA"
                     /locus_tag="PLZ54_01020"
     CDS             complement(15243..16553)
                     /gene="murA"
                     /locus_tag="PLZ54_01020"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOJ65555.1"
                     /translation="MSSFQIEGGHRLSGSITPQGAKNEALQVICATLLTSESVIIRNI
                     PDILDVSHLLSLLKDLGVKISKIEKGTYSFKADSLNMDYLQSEDFYDKSSSLRGSVMI
                     LGPLLARFGKALVPKPGGDKIGRRRLDTHFSGIQKLGADFRYNETKKQYEVSAKKLKG
                     TYMLLDEASVTGTANILMASVLAEGKTTIYNAACEPYIQQLCRMLIAMGAKISGVGSN
                     LLTIEGVKQLNGCEHTILPDMIEVGSFIGMAAMTGSEITIKNVAYDELGIIPDSFRRM
                     GIQVLQKNDDIYIPAHESYTIETFIDGSIMTIADAPWPGLTPDLLSVFLVIAAKANGS
                     VLIHQKMFESRLFFVDKLIDMGAQIILCDPHRATVIGLGNQLRLRASVMSSPDIRAGI
                     ALLIAALAADGTSIIHNIDQIDRGYEHIDTRLNALGANIERIQF"
     gene            complement(16604..17188)
                     /locus_tag="PLZ54_01025"
     CDS             complement(16604..17188)
                     /locus_tag="PLZ54_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOJ65556.1"
                     /translation="MEYTTLQGKLIMPEYGRNIQQMIAYAMTIEDREERTRCVKTIIN
                     IMGNLFPYLRDVNDFKHKLWDHVAIMSDFKLDIDFPYEVLRPENLYTRPEKIPYKNSD
                     IRYRHYGRTLEEMIEKVADYPDGEEKNELIRLIANQMKKCFLTWNKEVVDDRKIFDDL
                     RELSKEKLDISEDFLKLVESKEVLHNKKNKKKNK"
     gene            complement(17543..18271)
                     /locus_tag="PLZ54_01030"
     CDS             complement(17543..18271)
                     /locus_tag="PLZ54_01030"
                     /inference="COORDINATES: protein motif:HMM:NF026067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4465 domain-containing protein"
                     /protein_id="HOJ65557.1"
                     /translation="MKKKSLIVLSTLLLIFFTACENDSDFKLKTTVDFEDVELGENGY
                     WNGSDFSKKFVSNGFIFLNDFTSSPWGDFWLGFACSSLTDTITAGYDNQYSVAAGSGA
                     WGSNKFAVAFDDSATVNCPKNELGTYEAKSIMLCNSTYAYLDMRNGSAFSKKFDDGDW
                     FKVTIKGFLNNNATGAVDYYLADFRNGKQYLCHQWVKVDLSSLGKVDQLIFLFDSSDK
                     GTFGVNTPKYVCIDNLEFEQDAEN"
     regulatory      complement(18437..18656)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            18837..19619
                     /gene="ubiE"
                     /locus_tag="PLZ54_01035"
     CDS             18837..19619
                     /gene="ubiE"
                     /locus_tag="PLZ54_01035"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: protein motif:HMM:NF001244.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOJ65558.1"
                     /translation="MNKEKTTSQYTFQEVKPYNEKEGKTEQLVRMFDTIAAQYDKFND
                     LMSWGFAKLWRKRALSTLKRFRPKHILDVAAGTADMCIKAFRLLNPDKVTGIDISPKM
                     LEIGKEKIEHAQLSDKIELKVMDCAQLNFDDNSFDAVTIAFGVRNFEKLEESLHQIFR
                     VLKPGGHLLILELNEPQKGWLMKGYQLYIKLFVHQTAKYLSSDQNAYRYLTSSMHAFP
                     KGKEMIEILQNNGFELVECKKFTFGVCSMYLMEKPTHSKIDG"
     gene            complement(19679..20926)
                     /locus_tag="PLZ54_01040"
     CDS             complement(19679..20926)
                     /locus_tag="PLZ54_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOJ65559.1"
                     /translation="MKKLTISFCFYLIVLSVSAQLVATDKYAVYQNFTGVDYIFVFNG
                     ILPGTEIRFTGSGDNVKWYRFSDTTTPYSTLHYIFPEDASGYILDVDGHQTSVWVIDY
                     QNYLPVFHTLASDESSDNRCNELTLLLDANVPELYYYTLSGIRRKINRNFTLSYQTLE
                     WDNEWKTVETTKNVTLPVTEITVPAPLCNTTFSLQGDQFTSEPSLGIQPIGIISSMYA
                     AVSLECHIKTSTTARTEKNEDERPTSDALSGSAPLEMLFNAEVNNAGTIFYKWDIYKD
                     NQLLVSRSDKEHRYTFTEAGTYNVKLTVNNDFCSSSDSVSISVSTSDLQVPNVFTPNG
                     DGINDEFRVAYKSLISFDARIYNRWGRMVYHWNDPQKGWDGTIGGKKAPVGAYFYVIK
                     AIGADGIKYSKKGDINLLRGREN"
     gene            21123..23060
                     /locus_tag="PLZ54_01045"
     CDS             21123..23060
                     /locus_tag="PLZ54_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOJ65560.1"
                     /translation="MKKNIFCLLTVIILSLTGFSMEQTNSYPIKKVNGIEYYVYTVQS
                     GDGLYAISRKFGIAPEEISKVNPEIQKGLKSGQKILIPIQKNVQENEDASQRLKNELS
                     LLQEKSFEENQKNLHQQTDNSLPSTTLFLQHKVEKNQTLFGISKKYNVSEEEIIQYNP
                     QSKNILREGDVLFIPYKTQTTKNETEIKGDLVEVITPSGKKELNEKQLPSKDKPQYIK
                     HLVKPNETLFSISRTYNVTIDDILKLNPEAFPVLKIGTELTIPTSILKKDSIPSTTLN
                     NNEMDSALQDIENPEITTALFPENNIKIAFLLPFNLDTPSQQNANGRFRDFYAGSLLA
                     IKKAKENNYSLEIFTFDTEKSDEKLKEILAKPEMKSVDLIVGPAYSNQISLISDFAKE
                     NKINVLIPFSSKVYEIGINPYLFQFNPNDTQIEIDFLVDKIIQKTYRNANIVFVNLPG
                     ISILDEGNLFMERLKNRLLKERHKFNEFNFFSSNKNQSIPEVLKRNQKNLLFFNTDNL
                     SLITPFLKEFYVYIDEFDMMLYQEYGWENQYIERFDRIYVSPFKPEIDASTLEEYQKT
                     FHQFFEWEVESERPRYDLLGYDLLNCFIPILKNYENHLTKKLKEITFDEGIQSDFHFK
                     KQSEFSGYINDKLYMGERKAK"
     gene            23065..23694
                     /locus_tag="PLZ54_01050"
     CDS             23065..23694
                     /locus_tag="PLZ54_01050"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOJ65561.1"
                     /translation="MRKISIFLCIFLFSANLFAQPSSEKPEIYFGTTQGISASMINFS
                     PSVNQGMLLGYNGGLIFRHITEKNVGLQVELNFFQRGWKETDSIYSRRLNYIELPFMT
                     HIYFGNKSRFFFNIGPKISYLLTENVLKNETVSSEKVQHVKSIENPFDYGICGGLGFM
                     FRIRKNVLQLDARANYSLSDIFSNNKTDFFDSSNNINFSVNLGWLWQVK"
     gene            23979..24821
                     /gene="kduI"
                     /locus_tag="PLZ54_01055"
     CDS             23979..24821
                     /gene="kduI"
                     /locus_tag="PLZ54_01055"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018665715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HOJ65562.1"
                     /translation="MKTNYEIRYASHPQDAKNYDTSRLRKEFLVERIFTINEVNMVYS
                     LFDRMLVGGAMPVAEVLTLESIDPLKSDFFLERREIGIFNVGGKGCITVDETSYELDY
                     KEALYIGRGNKKVTFESVDGSNPAKFYFNSATAHKACPTRKITKENAVILEMGSLEGS
                     NHRFINRMMVSQVLETCQLQMGMTELEPGSVWNTMPAHTHSRRMEAYFYFELPDDQAV
                     CHFMGEPEETRHIWMKNDQAVISPQWSIHSAAATSNYTFIWGMAGENLDYDDQDFYKK
                     TDLK"
     gene            24901..25680
                     /locus_tag="PLZ54_01060"
     CDS             24901..25680
                     /locus_tag="PLZ54_01060"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HOJ65563.1"
                     /translation="MNQFDLKGKIALITGASYGIGYAIAKAFHAAGAVIVFNDINQEK
                     VDMGLASYKADGIDAHGYVFDVTDEEAVTEAIAKITKEVGIIDILVNNAGIIKRIPAL
                     EMSAKDFKQVVDIDLNGPFIVSKAVAPGMIAKGGGKIINICSMMSELGRETVSAYAAA
                     KGGLKMLTKNLACEWGAFNIQVNGIGPGYISTPQTAPLREEGHPFNTFIVTRTPAGRW
                     GTPEDLQGPAVFLASSASDFVNGHILYVDGGILAYLGKQPS"
     gene            25755..26885
                     /locus_tag="PLZ54_01065"
     CDS             25755..26885
                     /locus_tag="PLZ54_01065"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HOJ65564.1"
                     /translation="MKKIFLYLATVLMAGCSSSIKTIEISNTGSFDRNDEIIQINDKE
                     FLQLFEESDKWILKNNHNQEIAYQLVKNENGNLETFIFQAAVPANKKVAYHLKKGNPS
                     PVEPKTFARFVPERKDDFAWENDLAAYRMYGPALAGENPSNGVDLWLKRTKELIVDTF
                     YFNELQKGQSYHIDHGKGLDCYKVGHTLGAGGIAPFWNDSLWIGNYFNSYKVHYVGPL
                     QSKFSLFYDSIKVGDKFYKEQLTITVSAGTLLNKAEVTFTGYQQSLPLAAGIFLHENT
                     GKLSMNQEEGTAAYCEDAVSDAKVPSGRNYVGIFVPAPITKAMIKNNHALLISEYPPG
                     NTFVYFFGGGWNKWGFPTDNDWINAVNLFAQSYRSPLEVLIK"
     gene            27303..27941
                     /locus_tag="PLZ54_01070"
     CDS             27303..27941
                     /locus_tag="PLZ54_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005657281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOJ65565.1"
                     /translation="MNQRFVINIGRQLGSGGHLIGQGIARELGISFYDKELIQIASQE
                     SGLAKEFLEQLDEKKGQGFFRGMFGLRNSIMIDEYYFNDNYPSSEMLFKIQSDVIRSL
                     AEKQSCVFVGRCADYVLKDHPRAVNIFITADLPDRIARVCRNQKMTEEKAMDYIKKMD
                     KKRADYYNYYTDKQWGAAASYHLCINSSALGIEETISYIIEFVEKKLHFDKK"
     gene            complement(28122..28961)
                     /gene="prmC"
                     /locus_tag="PLZ54_01075"
     CDS             complement(28122..28961)
                     /gene="prmC"
                     /locus_tag="PLZ54_01075"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOJ65566.1"
                     /translation="MENILQYIHSELKGIYPESEIRFIGYLLLEKLTGFSRTEILLNK
                     NTQISCEAAKQLDGFLKKLKNHEPLQYVLGATEFCGLSFNVNPSVLIPRPETEELVEW
                     ICSENDRSKPLKMLDIGTGSGCIAISLKKNFPLAEVDAFDVSPEALKVAKENAKLNRV
                     NVNFREIDIFNPPVFSKKWDLIVSNPPYVPENEKSEMQRNVTDYEPSLALFVPDNHPL
                     KFYETITNFARLNLAKGGKLYFEIHYRSGKNMLDLLSQNGFREVQLKKDLSGKDRMIS
                     GIF"
     gene            29051..30106
                     /gene="ribD"
                     /locus_tag="PLZ54_01080"
     CDS             29051..30106
                     /gene="ribD"
                     /locus_tag="PLZ54_01080"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005926755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOJ65567.1"
                     /translation="MDIEIKYMQRCLQLASLGDGYTAPNPLVGAVVVYNDTIIGEGFH
                     QHFGQAHAERNAIFSVKQPELLKDATLYVNLEPCSHYGNTPPCASLIVEKGIPRVVIG
                     TIDPNPKVSGKGIQILKDAGIETYVGVLEEKCRELNKRFFTFQEQKRPFIVLKWAQTI
                     DGFIDRIRNNVSEPPLIISNEITKQLTHKMRSENQSIMVSTNTVLLDNPSLTVRNWIG
                     KTPIRITLDRQGRIPENYHLLDGLVPTLVFTERQPAKLNNVEYIQIDFKKEGLRGILK
                     EIFDRNIHSVLVEGGAQLLNSFIAENLWDEANVEVAPIEIGNGIAAPVMTCQPASRKN
                     FDGHEWLHFYNPKKTIK"
     gene            30107..31531
                     /gene="cls"
                     /locus_tag="PLZ54_01085"
     CDS             30107..31531
                     /gene="cls"
                     /locus_tag="PLZ54_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOJ65568.1"
                     /translation="MSEIISIFFIALYVYTILTTISVLLLENRNPSKSLSWMLIILFL
                     PVIGLILYLIFGQDLRKQKIISRKSIHSLDHRDISSFDFSKINSDLLDAHQMNLIKLL
                     YRNSEAVGYTYNKIEVFSDGKEAFESIFQAVEQAREHIHLEFYIISDDSITNRLKDLL
                     IKKSHEGVRIRIIYDFIGSFNLSRKFLKTLTKAGIYVRPFFPLRFRISRSRINYRNHR
                     KLLVVDGKVAFTGGMNIADRYVYGNRLGYWRDTFLKIQGSAVHGLQHLFLSDWYFVEK
                     KLITDKKYYPVPAQFDNNLVQIVSSGPDTEWEAIMQGIAKAIMSAKKYIYIQSPYYMP
                     TELIEGCLQLAALSGIDVRIMIPTQSDSPIVIASTSSYLGKVLQAGVRVFYYRKGFLH
                     AKTVVMDDFVSIVGSCNIDYRSFYQNFEANAFVYEEKTALTLKELFLEDIHYCEEITL
                     EQWNNRKKTKKLMDSVSRLFSPLL"
     gene            complement(31556..33073)
                     /locus_tag="PLZ54_01090"
     CDS             complement(31556..33073)
                     /locus_tag="PLZ54_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ65569.1"
                     /translation="MRKFRLSVVLLFFAFSFLQAQPKREMRAVWIATVANIDWPSSNR
                     LLPTQQRIEIVNMLDRLTENNINAIVLQIRPMADAFYPSNLEPWSHWLTGRQGVRPSP
                     FYDPLQFIIEEAHKRCMEVHVWLNPYRVTTTGDSMMLSKEHLFYKQPELFVRYGGKYY
                     FNPGLDETRNFLNKVVEDIVEHYDVDGVHFDDYFYPYPVKGEDFPDDETFMKFPRGFA
                     PNQKADWRRNNVNMAIAEVQQTIKNLKPWVEFGVSPFGVWRNDNVDPRGSATRAGIQN
                     YDDLYADILKWLAEGTIDYVTPQLYWEIGKKVANYEVLLKWWIENSYGKNLYIGLIPS
                     SLGDKKNPAWTTGNEIIRQIKMNRNSSQVDGAVFFSAKTFLQNRQGLNDSLQSNYYRY
                     PALCPINRNILGEPSAQPQNLRVLRDGKDAFLLWDEVNEEGGCQTAYYVVYAFKGKEV
                     GDLNDPVNIIARTTENCIDLRILPHKFKGNYTFVVTAVNRFRQESIPTQGITRKL"
     gene            complement(33094..35887)
                     /locus_tag="PLZ54_01095"
                     /pseudo
     CDS             complement(33094..35887)
                     /locus_tag="PLZ54_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
     gene            complement(35897..37123)
                     /locus_tag="PLZ54_01100"
     CDS             complement(35897..37123)
                     /locus_tag="PLZ54_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOJ65570.1"
                     /translation="MQKLIIFIFSVLFAFPLYAKRVMTGIEVLRADNFKLLEGKRVGL
                     ITNPTGVDGNLKSTIDILYEARNVKLVALYGPEHGVRGDVYAGDKVVNATDEKTGLPV
                     YSLYGTTRKPTSEMLKGIDVLVYDIQDVGCRSYTYISTMGLAMEAAAENDIEFIVLDR
                     PNPLGGEKIEGNLTEEPFISFVSQFKIPYIYGQTCGELALMLNEENALKKKCKLTVVK
                     MKNWKRSMKWEDTGLEWVIASPHIPHKHSAYYYPVTGILGELGYVSVGVGYTLPFQVV
                     AAPWIKAQEFADAMNRLHLPGFIFRPIFFTPFYGTLKGENCQGVQIHIVDFKKAHLSL
                     VQFYIMQELARLYPDKKVFDNANKERFSMFDKVSGTDQMRKLFQVNNSVSDIQHYWDK
                     DIQSYYQLSKKYYLYH"
     gene            37421..39316
                     /gene="mutA"
                     /locus_tag="PLZ54_01105"
     CDS             37421..39316
                     /gene="mutA"
                     /locus_tag="PLZ54_01105"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase small subunit"
                     /protein_id="HOJ65571.1"
                     /translation="MAEKKLNLLADFPPVSTQEWLDKIIADLKGADFEKKMIWRTNEG
                     FNVRPFYREEDVDDLKITELLPGAFPYVRGTKSDNEWFVRQDIKVENAQEANAKALYI
                     LNRGVNSIGFQLKKADLSKEFVATLLKDIAADCVELNFTICISKAAELADILSEYFIA
                     QNYEVKKLQGSINFDPINRMLLAGKKLTKEEIAEKAKAVVQAISRLPFYRVIGVNATT
                     LSNAGSTITQELGYALAWGNEYLSMLIDAGVDKAVAAKKIKFNFGIGSNYFMEIAKFR
                     AARMLWALIVDAYQPPCPRPDCPNTVNGICKCAAKMRIHAETTIFNQTIYDAYVNMLR
                     TQTEAMSATIGGVDSLSVLGYDTAFKPSDEFSERIARNQQLLLKEEAHFSEVVDPAAG
                     SYYIETLTAEIANQAWKLFLNVENEGGFLAAVQNGKVQQTIKATANERLKAISNRKEI
                     LVGTNQYPNLLETAAEKIDKELQQCGCCSHEEGTEKLLPLRGAVEFENLRLAVEKTSK
                     RPKVFMLTVGNLAMRSARAQFSGNFFGCAGYEIIDNLGFSSVEEGVKAALDAKAEIVV
                     LCSSDEEYATLAPEAYKLLKDKTIFVVAGAPACMEDLKKEGIENFISIKSNVLETLKD
                     FNKKLGL"
     gene            39393..40295
                     /locus_tag="PLZ54_01110"
     CDS             39393..40295
                     /locus_tag="PLZ54_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HOJ65572.1"
                     /translation="MKEKIKNGLLATRPWSFPASTMPALLTISYVFFLKNELGSEVNW
                     WFGVLALLGAAIFQASGNLIGDYFDYQHHVDRKETFGSSRMLVDQVFQPKTILHFGIV
                     LLAVGVVLGLFLCYRTGIDLLWIGAIGAMATYFYYKLKFAALGDLTIFIIYGPLIGLG
                     TAYVMTGQLMWFAVVLNIPVAFLVVNILHANNTRDIRDDGKANIKTQAMLLGIKGAKI
                     QYVVLSIGAYVGVVLMIIFKMIHPVALLMLLSLPLAVKNIKKMMTAEVEKPEKIFDLD
                     AQTAQLVLVFSLLFSISNFIAAWL"
     gene            40292..40906
                     /locus_tag="PLZ54_01115"
     CDS             40292..40906
                     /locus_tag="PLZ54_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005823820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOJ65573.1"
                     /translation="MKRIIIPILIAIIFWFVMFSPWTKEYVNFWLTMTAAGITLTLMS
                     AFLGKDFKKQFSLSLKDVLIGLGSAVVLYVVFFLGDFFSKLLFDFAKDQVGQIYMMKE
                     GENPLLLSLLLILLIGPAEEIFWRGYVQRMLEPKFGSWMALIVTTLIYTLVHIWSFNF
                     MLIMSAMVCGAFWGLLYKYNKNLVTLIVSHAVWDVSVFILFPIV"
     gene            41081..43210
                     /gene="scpA"
                     /locus_tag="PLZ54_01120"
     CDS             41081..43210
                     /gene="scpA"
                     /locus_tag="PLZ54_01120"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOJ65574.1"
                     /translation="MKPNFNNIDVSNITSSGAAMLQTEENWLTPEQIPVKPFYEKNDW
                     KGMEHLHYAAGIPPFLRGPYSSMYVTRPWTIRQYAGFSTAEESNAFYRRNLAAGQKGL
                     SVAFDLPTHRGYDADHERVVGDVGKAGVSICSVEDMKILFDGIPLNKMSVSMTMNGAV
                     LPILAFYIVAGLEQGAKLEELSGTIQNDILKEFMVRNTYIYPPQFSMRIIADIFKYTS
                     QNMPKFNSISISGYHMQEAGATADLELAYTLADGLEYLRTGINAGLDIDAFAPRLSFF
                     WGIGMNHFMEIAKMRAARLLWAKIVKQFHPKNPKSMALRTHSQTSGWSLTEQDPFNNV
                     GRTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETNICRQIDPW
                     AGSYYVESLTKELAEKAWDLIQEIENLGGMAKAIETGIPKMKIEEAAARTQARIDSGK
                     QIIVGVNRYTLEKEEPIEILEIDNTAVRMQQIERLKQLRASRNNEEVKKALDAITECA
                     RTGNGNLLELAVEAARVRATLGEISDACEKVAGRYKAIIRTISNVYSSETKNDANFQR
                     ASQLTEEFAKKEGRRPRIMIAKMGQDGHDRGAKVVATGYADCGFDVDMGPLFQTPAEA
                     AKQAVENDVHVVGVSSLAAGHKTLIPQLIEELKRLGREDILVVAGGVIPPQDYDFLYK
                     AGVAAIFGPGTPVTKAACTIMEMLLAD"
BASE COUNT        13882 a         8315 c         8142 g        13114 t
ORIGIN      
        1 gaaaatttgt aatttgcctt gcaaggtttt aaaatgcaac acgaaataac ttatgcgatt
       61 tgaagaaata atcatagaat taagttacaa ctgcaatatg agttgaataa tgtgcggatt
      121 tggtatagac gcaaatccat tcaataaaaa caaatttttg tcttttgaga aatacaaaac
      181 aattttgcac caaattggcg acaagacaaa aacaatccgc ctaaatggtc gtggagaaag
      241 cacaatacac cccgattttg ttgagatttt gaattacaca aaacaacatt ttccgcaact
      301 caatataaat ttgttttcaa atttttcatt caataacaaa aaaattattg atgctttgat
      361 tgcaaatggt gttcaacttt ttatttcaat ggacagcccg aaagcagatg aattgtcagc
      421 aatcagaaaa ggagcaaatt ttcaatttat tgaaaataat atcaagcaac tccaagagtt
      481 gcccaatcgc ccttttgtta tttttactat acaagaggcg aatataaacc gtatttttga
      541 aatggcggag tttgcgtttg aaaataattg tcaaattttg tataacacca ttcgcagaga
      601 tgaaggcgtt gaagtattta ttgaaattgt caaacaaaat tatcagtcaa ttttagaaca
      661 atttaagcaa atcggaaaat tatacgctaa ttcagaattg caattttttt atcccgacca
      721 actcgcaggt gttgaattgc aaaccgaaaa accgactaaa acgcacggaa caatgaaaca
      781 atgtcctgct ttagaaaaag aattgtgcat tttgtacgac ggtacagcaa caccctgtaa
      841 tatgtttaat ccatacactt acggaaatat ttttaatcaa tcgttaagtg aaatatggga
      901 aagtaaagaa agaaaaattt ttttgagttc tcacaagtgt cattattatt gtcagaattg
      961 tgcaaattta ggagtttaaa atatgagtta tttatcaaat gttccatacg gcaagatttt
     1021 atattattgg tacgaaaaat tatcgcataa tccatacaga aagaagttct tatggactaa
     1081 aattattgaa ataatcgcct tcaaacggca tagtagcaag caaaacaata aaatttatca
     1141 atatgtagat tttctgcaac caatcgcaaa cgacatttcg gaaaatccga ttgtcagcat
     1201 tgttgtgccg acttttatta gaaatcaaaa agatataaat gacattgaaa atcttttgct
     1261 gtctatttca aagcaaacgg taaaacccga caatattatt atcgtagatg atttttcgcc
     1321 aatttcttat tcattccccg aacacataca agtttatcga caaaccaaaa attcgggacc
     1381 agcgaaagca cgaaattggg ccaaaaaaaa tgctgttgaa aataaatcgg acattatcgc
     1441 ttttaccgat accgattgca tactttcaga aaattgggtt gaaacgataa taaaatcatt
     1501 tcagcagtca aaagattttc agatattgtc gggcaacaca atttcttatg ataaaaattg
     1561 gtttggaata tatcacaata ttaacggaac tttgaacggt cgaaaattca aagacagtga
     1621 gcgacttttg tacggaacaa cagcaaattt gtctacaact cgacaagttg ctgaaaatat
     1681 tgattttagc gaagagtttt cgttagccgc aggcgaagac attgagtttt gttttcgggc
     1741 aaatcacgat tgttttgcga taaaacacat tcctcaaatg attgtttttc acaattacgg
     1801 ttacacaaac ggactttgga aaaacctaaa atcatttact cgtcaattca tgagatacgg
     1861 acagggcgaa gttactttat tacaagaaat tccagaatat tacgtttatt ttgacaaaac
     1921 agaagaaata acagcagtat gaaacaaatt ttaataacag gcggtgcagg ttttgtcggc
     1981 tctcatttgt gcgagagatt actaaacgaa ggaaataaag taatttgttt agataatttt
     2041 ttaacaggta acaagcaaaa tatcaatcat ttaatagaaa acccaaattt taaaattgta
     2101 gaacacgaca tttgcgagcc gtattttacg gaaattgacg aaatttataa tttggcgtgt
     2161 cctgcctcgc ctattcatta tcaatacgat gctataaaaa ccgtcaaaac ttcggtatta
     2221 ggaacaatta atgttttggg gttggcaaag cggacaaatg cgaaaatttt acaggcttcc
     2281 acgagcgaaa tctacggcga cccaattgcg caccctcaaa acgaaagtta ttggggaaat
     2341 gtaaatccga ttggtattcg ttcttgctat gacgagggca aacgctgtgc cgaaacgcta
     2401 tttatggatt accaccgaca aaacaatgtt cgtatcaaaa tagcaagaat tttcaacact
     2461 tacggaactc atatgcaagc aaatgacggt cgagttgttt caaattttat cgttgaagca
     2521 ttgcaaaaca aggatataac catttacgga aacggtcaac aaacccgaag ttttcaatat
     2581 gttgatgatc taattgacgg actgattcgt ttaatgaaca cgcgtgacaa cttcgtttcg
     2641 cctgtaaata tcggcaatcc aacagagttt acaattttgg aattagcgga aaaaataata
     2701 gatttgacaa attctaaatc aaaaatcaaa ttcttttcag ccgttcaaga cgacccgaaa
     2761 caacgctgtc ctgacatttc gttagcgaaa gaagttttga attggcaacc aaaaattgac
     2821 cttgaaagcg gattgaaaaa aacaattgag tattttgaaa atatactaac cgattaaaga
     2881 ctaaatccac tgctgctaac acacgcctat ggtcacaggc tggctgacgt gtaatttgga
     2941 ggttttgctc ccgcattcac tttgtagctt ggcgacagtg aacgctcccg caagccgcca
     3001 atgtccatag actcgggcgt taggggtcag gctaaacgat aatgcaaacg agacagaaag
     3061 tgcaatgagt aaaagttatc ttatagtatt tcacggacaa atcgttaacg acaaaatcgt
     3121 tgacattggt gacgacccat gttttgactg tccaccaagt tggggcattt gcagaccatc
     3181 gacaagaaaa agtgtgaaag ttggagatac tttaattttt attgcaaagg ttgacaacga
     3241 ctattttcta aaaggatggt ttaaggttgg caataaattg gactatccct ttgctttaca
     3301 acaatttcct tcaagacaaa atgttattat ttctaaaaca atttcaagcc accccatcaa
     3361 atggcgttat aaaaacttac agaaaagtta ctacaagaca cacggacaaa caacgccaaa
     3421 tttcttaatc caacttgata caggtagtgg aactttttat caaagccaaa ctgacgacca
     3481 cgaaacggac aattggaagt gtagaagaat ttttcattgc acttctaaac aatttgagaa
     3541 atgtatagcc gcaaacaatt gtttaaaaag tagtgaatta cttacagcta atcaatacaa
     3601 aaattatgtt gttgctgaca aaaatcattg ggctgacttg gataatttga gaatcacttt
     3661 tgatgaaata aaaaaagcaa caaactttcc gaaaaaaatt cggacaccaa aaggacaaca
     3721 caatgttatg cggtttgacg attataaaga aaaacttttt gcattcattg aaaaaaggaa
     3781 aaatgatgct aaaaaatagg caacataatt tcggacagac agaagcccat accctggtcc
     3841 gcgggcaagt agatggcccg tttgagtatt tgcattttaa cgggaataaa ttggtgaaaa
     3901 ttgatgtgat taagaatgtg tttagataat gtgatattaa acaatcaatc ataaataaaa
     3961 aaaatgaaac attggatatt tattgcaaat gacaacgatt gggattggcg aaccaatcaa
     4021 aaagttggtg aaattgaaaa ttggcctgca tatggaaaaa gagactcaaa agaaattttt
     4081 caaagctaca agtaggcgat aaagtaattg gatatttctc agggaaatca aaatcttttg
     4141 tttcaattgg agtgattgaa aaatctcttt cacaagatcc aaataaaaaa gaaattgcta
     4201 ttagaaaaat ctgcgattta caaagtccta tgagtcttgg cagagtacgg caaataaaac
     4261 cttttgacaa taaattaagt aataatggta agctaaatac aaccgtaatt cctttaaccc
     4321 ttgatgagta taatcttcta atgtcaatta ttcacggtgc ttttttagat gtttatgatg
     4381 atgttgattc cgatttaaac tcggtggata tgccgattaa agaagcttta aaactcagac
     4441 cagccaagca gataagtaat tcaaccacat ggattcggga ttcaagaatt tcaaaagcag
     4501 ctctgaaaca cgcaaactat atttgtgaat ttgctactaa atcagataac catgaaagtt
     4561 ttattgcaaa ttacacaaaa aaaattatgt tgaagcacac catttgattc cgatgaaaga
     4621 gcaagaaaat ttcaaatact cacttgataa tgcaaggaat attattgctt tatgtccaaa
     4681 ttgtcacaga atgatacatt actctgaaca atcgattcgg aaagcgaaaa ttactgaaat
     4741 atttgaaaaa cgaaaaaaag atttagatgc ttacaagata actttatccg atttatatcg
     4801 aatgtataat gtatagaaag atgtgagaaa gccttctaaa aggtcaaaaa accagccaac
     4861 aactattgta taagtaatgg cagggtaagt ggttaatatc aagggttgta gccatcgaag
     4921 cgaggatttg gaatccagta ttgctaatta cttagacgtt tatttttttc aaaattcaat
     4981 tcctgataat tatagatgaa attgcaaatt ccatcgacct ggaaaatcag cacctattcc
     5041 aagaaacata gcaggttaca ggaatctgta agttttcttt ttcatccaac caacaatcaa
     5101 ttattgtaga tcgaataatt taccgctaac attgctccga atcccaaatt cttgccgttg
     5161 gcccaccttg tttcattcat caaacaacgc ttcgaaatcg ctgaatgaaa ccgataaaaa
     5221 tttttagtac aaatcttgta aattcaaaac taatatctta agtttgaagt cggataccga
     5281 cattttttcc aattaaaact tttcaaagaa aaacatcatc aatattctaa aaacaggagg
     5341 atatcactat ggcagacgta attaaaaatg cttttttggc agggctggga ttggtttcgc
     5401 ttacgcgaga aaaagccgaa gaatttgcta aagacctgat taaacgcgga gaattaaccg
     5461 aaacggagaa acctaaattt atcaaagatt tgctggaaca atcagaaaaa accaaaaccg
     5521 aaatggagga aaaggttgaa aaggcagtcg atgcaattct aaaaaaaatg aatattcctt
     5581 cacgaaaaga attcgaagaa ctgaaaaaca aagtagaaga gttgacgcaa ctgctcaaca
     5641 aaaaacagga agaatcagaa aattaaacca atgaacgatt cctatccata caggaatttc
     5701 ggtttcagaa acaacttgga taaagtcaaa cgaagccgtc agatcatttc cgtttttgtg
     5761 aaatttggtc tggattatct ttttgaagct tctcgagtga actttctttc aaaattgagg
     5821 aaacgacgaa aagtttatca caaactttca actgccgaaa ggcttcgtct ggcttttgaa
     5881 gaattgggcc caactttcat aaaatttggg caaatactca gtacccgtcc cgattttctt
     5941 cctgcggaat atattcgtga attggaaaaa cttcaggatc aggttttacc tatggaaact
     6001 ccgctggtga ggcggtgtat tgaaaaagag ctgaaacata cccttgaaga actttttttg
     6061 gatttcgacc aatcgccggt tgcttccgct tctttaagtc aggtgcacaa agcagttctg
     6121 caaaatggcg atacggtagc cctgaaaatt caacggcctg atattcataa aaccatcgaa
     6181 acagacctct ccatcctcga aagctttgcc gaaatactgg aaaaaagact tcgcaacagt
     6241 tccctgtatc atccaactgc aattgtggcc gaactgaaga aaaccatacg aaaagaactg
     6301 gatttcatga atgaagccca caactttgaa aaattcagga aaaatttcaa aaatgtggat
     6361 tacatacaag ttccaaaagt gtattggcaa tttactaccg agaaattact gacaatggaa
     6421 ttcattgaag gagtgaaaat taccgaaatc acacaggaaa aatacgaaca ccgctacgag
     6481 cccaaaaaag ttgccgacag agcggcaaaa gcattgctga aacaaattct gatagatggt
     6541 tttttccatg ccgatccgca tccgggaaat ctttttatcc aacctcctgc caccatcgtg
     6601 atgttggatg tgggtatgac gggacatctc gacgaagaat ccatcattac cattgcccga
     6661 attttacgtg ccgtcaacga aaaagacagc aatcacgtaa tcaagggctt acaagaactt
     6721 ggaatcgtgg ttaaagaaac cgacaagcgt caattgcggc acgacctcac cgaattgatc
     6781 gaaatgtatg ccgacagacc attcagaacc attagcctga accggatgaa ccagcagttg
     6841 ctggaaatta tggctcgtca tgacatggcg cttccttcca atttggtgat gatgctgcga
     6901 gccatgtcta tcgccgaatc aatcgggcag caactggagc ctgacttcga tatgtttgcc
     6961 attgttgagc cgtttgtgaa aagaatttat ctgaaactct attctccaaa aagtttcaga
     7021 aaacgcagca tgaacgtgat ggatgagagc attgaactaa tagaactttt tccacaaaat
     7081 atatccgatt tgcttggaaa gttgaaagag ggacagtttg aaatcaaact ggaacatcaa
     7141 aacctcgaaa atttgcagga tgaaatgaaa agttcgagca acagaatatc ggccagtttg
     7201 attattgcgg cactgattgt aggttcttcg ttaattttgc aacaaagaat cggccccctg
     7261 atttccgata tttccatttt gggcgttatc ggttacttgt tggccggcat catgggttta
     7321 ggacttcttt tgaccatttt caaaacccgc agaaaaaaat aagttttcct acatggtttg
     7381 aaatctgatt tttacttcct tacctaataa accttttcac catttgggct tcattcccaa
     7441 attatacatt acaaacgacc acatatctgt ttgtgcatca attagttttc ccaaaggttt
     7501 ccccgctccg tgtcccgcat tgttatccac gcgaatcaat acaggcttat taccggcatt
     7561 ggcttcctgc aaagtagcag caaatttgaa tgaatgagcc ggcacaacac ggtcatcgtg
     7621 atcacctgtc ataatcagaa ctgcaggata ttttgtgcct tttaccacat tatgcaacgg
     7681 agaataggat ttcagatatt caaacatttc cctgctttct tcgctcgtac cgtaatcgct
     7741 tgcccacgac cagccaatag taaacaaatg gtaacgaagc atatccagca cacctacttc
     7801 aggaacagcc actgcaaaca aatcgggacg ttgcgtcaaa caagctccta ccaacaaacc
     7861 gccatttgaa ccaccattaa tggccagttt ttttgaagaa gtataacctt ctgcaattaa
     7921 aaattccgcc gcggcaataa aatcatcgaa aacgttcttt ttctgcattt ttgttcctgc
     7981 catgtgccac gattctccat actctccgcc accacgaatg ttggcaacgg cataaatacc
     8041 tccattttcg agaaaaggaa tacgcgaaat actgaaagaa ggcgtcatgc tgatattgaa
     8101 acctccataa ccataaagca tcaaaggatt ttttccgtct ttttttattc ccttgcgata
     8161 ggaaagaaac atgggaattt ttgtaccgtc cttagccgta taaaaaacct gttcggtaac
     8221 aaaatcttcc ggcttaaatt tcacctgcgc tttttgaaaa atttcggaaa cattcttgcc
     8281 ggcatccaag cgataaatgg taggtggata ggtaaaagaa ctgaaagcaa aaaaagcctc
     8341 actgtcgtct ttgtttcccg tcaggcttac aaccgagcca agtgccggca attttacttc
     8401 gtaaagaagt ttcccgctta aatcgtaagc atacagacga tgagcagcat ctttcagata
     8461 aaggcaaaaa aactttccat cgataatttc aaccaattcg agtaccgttt tttgttcggg
     8521 aataatttca cgccagtttt cgcgcgaagg atgggccggg tcaacttcca taatgcgttt
     8581 attgggcgct ttccaattgg taagaatata aattttgccg tttatgtggt cgatgaccga
     8641 ataatcattt tcaaaaccat ccaccaatga aacaaattta gctttaggat cggttaaatc
     8701 cttcatcagc agagcgttgc cggtggttgt ttccgtaatg atcaaaaaaa gatatttttc
     8761 atcttccgta acaaatgggc caaattcgcg caacggatga tttttatcct gataaatcaa
     8821 caagtccttg ctttgaagtt gtcctatctg gtgataataa acttttggaa attcgttctt
     8881 tgccgacaat tccttgcctt tttgcggagc atcgtaggct atatagtaaa atccgttgtc
     8941 tttccatgca acattccaga attttgtcca ctgaatgtga tcggataaag tttgtcggtt
     9001 atccagattt aagacaaaaa tttcattcca atcagaaccg ctttttgcca ccgaataagc
     9061 catataattg ccaacgtttg aaaacgaaac ggatgacaag gcaaccgtac catcggaaga
     9121 aaaagtgttg ggatcgagca aaatttcagg ttccgaatat aaggttgact gctgataaag
     9181 aatactttgc ggttgtaatc cattattttt gaaaaaataa tacttgccgt gttttttaaa
     9241 cggagcaccc agtttaggat aatcgttcag ttgagtcaga cggtttttca atgcatttcg
     9301 aaaaggaatt ttttccagat atttgaaagt aacttcattc tgagctttta cccaagccgc
     9361 agttgctgcc gacgtatcat tttcgagcca gcgatatgga tcggcaacat cagtgccaaa
     9421 ataatgatcg gttacgtttt ccttttgggt atgtggataa gcaatttttg aaaaagaatg
     9481 acaagctgcc ataaaaacaa tggaaaaaga aaggataaaa actgctttca ttttactgta
     9541 aaattataaa aatattatgc aatcatttca atgcctgata gaccaaattg tggaacaaaa
     9601 ctttgacatc gggtacactg ttatcggttt gccaattgat ataaaaaagt aaaatcaaat
     9661 cttctttcgg atcgataaga taatcggttc cgaacatgcc accccaacgt aaagaacctt
     9721 cggaagccat tgtaagatgc aaatattgag gacgaaaatc gtcgaatgcc aaaccaaagc
     9781 caatttctcc gcgtaaatct ccaacctgat tgcgacacat caattcgacc gtttttcttc
     9841 ccagtatgcg tttcccgtta aattctccct tgttgagaat catttgacaa aattttgcat
     9901 aatcttcaat cgttcccgat aagccggcac cggtcgaaaa cagagtttga gcgccactgt
     9961 acggaaatgt ctgataaagc acacttgaac tgggctgcaa agttttgccc tgcacttttt
    10021 gataaagtgt aaccatacgg ttttgttttt ccttaggaac ataaaaataa gtatcgttca
    10081 ttccaagcgg cataaaaatt ttctccttaa aatattggtc aacaggcatt tttgaaacca
    10141 cttcaatcaa ataacccaac acgtcaacgc tcattccata ggtaaatctt tctccgggat
    10201 cgtgctccaa aggacaggaa gcaagttttt tcaccacttc tttcaatgta accggatcca
    10261 agctgttcaa gggtggaatt ccctttttag caaaaatttt catcataggg ttattgggat
    10321 ctgttccata agaaattcct gatgtgtgtg tcaacaaatg acgaatggta atatccttgt
    10381 ttgcaggcct ggtagtataa gtggaatctt tttcgttaaa cgacaccaga acttggggat
    10441 gggcaaattc gggaatatat ttttttaccg gatcatccaa aagaattttt ccttcttcca
    10501 tcaacatcag cacacctgtt gcggtaatag ctttggtttg agaagccata cgaaaaatat
    10561 cgtctctttg gcatggaata tttttctcct tatcgcgcca gccaaatgct ttgaaatgaa
    10621 caatgttgcc acgatgtgcc acaaaagtaa cagcatgagg aatttcgcct ttccttacca
    10681 aatcctgaaa aacagtgtcc aaccgagcca aacgttcggt atcgaaagca gcaaaacttc
    10741 tttcggaatg cttaaatttc agaggatttc cattattttt tgccattgtc gcacatgaca
    10801 aaagcaacag cggaataaaa taaaaaattt ttttcatttt tataacgttt ttaattggtt
    10861 aatcccattt ctgcggcaat ctgcatcata tagcttcgtg cagcttcata ttcgttggga
    10921 ataataccat ccagaatggc atccttaatt ttcattttaa tttctccaac caaagcacag
    10981 ggaggaagat gaaaagtttc catgatttct tttccatcaa tgggaggttg aaaattcctt
    11041 atcctgtctt tttcttctat ttccttcaac tttttccgaa ccagctgaaa attttccctg
    11101 taacgtttta ccttttccgg atttttagag gtaatatcgg cttcgcaaag cagcattagg
    11161 tcgtcaatgt cgtcgcccgc atcaaaaagc agtcttctga cggccgaatc ggtaacctct
    11221 tcttcactga gtacaatggg acgcatgtgc agggaaacca ttttctgaac atatttcatt
    11281 ttttcgttgg tgggaagctt cattcttctg aaaatatcgg ctgtcatttt ttcaccaact
    11341 atgttatgat tataaaaggt ccagccctga cgtttgtcaa agcgcttggt gcgcggtttt
    11401 cctatatcgt gaagcaaagc agcccatcgc agccaaagtg aatccgaagt ctgactgaga
    11461 ttgtccaaca ccatgagggt atgataaaaa atatctttgt gcccaatccc atctttggtt
    11521 tctacacctt tgagggccga aagttcgggt aaaataaaat ccaacaaacc cgacttgtcg
    11581 agcaaaataa agccgataga aggtttggga gaaagaataa ttttgttcaa ttcgtctgta
    11641 attctttctt tcgaaataat ttcgatgcgc ttcttattcc gaccaatggc ttcaaaagta
    11701 ttggtttcaa taaaaaaatt gagttgtgtc gcaaaacgaa tacatcgcat cattcgcaac
    11761 ggatcgtcgg aaaaagtaat atcgggattc aaaggggtgc gaataacttt gttttgcaaa
    11821 tccctgagac cgttgaatgg atcgactaat tcaccgaaac gacttttatt caggcacagg
    11881 gcaagggtgt tgatggtaaa atcccggcga ttctggtcgt cctgcaaagt tccatcttcc
    11941 acaatcggtt ttctcgaatc ggaacgatac gattccttgc gggcgccaac aaattcaaca
    12001 tccatttcct tcagtttgat ttgtgccgta ccaaaatttt taaaaatggc aaatttgcaa
    12061 ggcttgttca gttgcttcag ttgattgacc actttctctg ccaattcaat accattgccc
    12121 accacaagaa tatcaatatc ctttgatggt cggcgcagca gcaaatcgcg cacgaaaccg
    12181 ccaattacat aacattcctg atgtgcttca tcggcaatat ccgaaataat gttgaaaatt
    12241 tcattgttta aagcattttt caggttttcg attgtcggtt ctttggtaaa attttgttga
    12301 aaatctttta gggagtcggt tgtcggaaat gtgtcttcct gattcatgta gaaatctgat
    12361 atttaactta ataaaaataa ttaattgcct tccaaacaat tatatttatc gtttttctga
    12421 aatatgaatc ccacatgtat tgcatacaca aacacgaatg aatataatat tcagacaggt
    12481 gggttccatc agacctttaa aaaataaatt atattctgat gaaatttccc ttatacaaag
    12541 gtaggtcaat aaaattaaac ttcaaaaaac ctccatcatc caaaaaaact ggtgttgctt
    12601 ccaacaaaac catcgttaca aaataaaatg ttttgttcaa tcattatagt cagtcgatta
    12661 cgctgtaacc gatcaatcgg tcgtaacgtt caccccaagc ccgatggtaa aaataaatgg
    12721 tgtattcgtt tttagtttgc cagaaactgc cttcaatttt tccggttgaa gcagaatttt
    12781 taccttttgg caaaaaccag tattgataac tgtagccgcc ctgtttcagc aaaagcgttt
    12841 tggtataacg ttgagcatcg aaatcatatt gcattcgcgc tgtatcatcc agcaaattat
    12901 aattgtaatc gccaccaatg aatacctgtc catcaaaaaa cggttgttcc gaaagtaaag
    12961 aaaaatgtac ctgaacataa tcggcttccg tttgtacgtc ctgaaaagct tcctgaaaat
    13021 ttatgataaa tcggccattg gcatcaggtt gcgaaatata ggtacgcgac ttttgcatca
    13081 aatcttcctt aagaaaagct tcataatact tgcgattaaa aataattctg tccaccccct
    13141 ctcctgctgc ataaaaagac gaaatatcaa aatgacggta ttcgtttcct ccttcaaaaa
    13201 tcaatgcttt gttattgaca aaacttaact tcccggcatt gaaaaaagtc ggcgttatgt
    13261 ttgccacctc gttatcgaac ctgttatttt gtcgaacaac gagtttaact tccgaagcag
    13321 gatcctgaac aacataattt gccagattca cttctatgtc gagctgctga tagcgtccac
    13381 tcaattcgat atcggtattg gctctgacac gggcatctat ctctactttt ggctcaacaa
    13441 tggaaaaaca ggcagtggct accggatcat ccacacgatt gtcttcataa atgagtacca
    13501 catagtttcc ggaaatttta aaacgcatgt cttggttcgg tatcgtaaac cgataatgaa
    13561 tatattggaa agtagtattt tgcgaaaagg cgtaatcgtc gatattacca cgcgtaaaac
    13621 catcgacata ttcgttgctg tttaaattgg aaaccgtgca gtcagcattg caatgcaaaa
    13681 ccgtgtaacc gaaagagcga acattgtgcg acatttcatc aaactccaca gtaagtgttt
    13741 ctgttccatt taatttcaaa atggggaaac cgaagtcggt atccgaaatt ctgatttgca
    13801 aagttttgat attgggtttc aaaattttgg ttcgataggg attggcggct gttaaactag
    13861 aattaacaaa taaaaaaata aaaacaacag tggacaaaat atttttcatg attctcacag
    13921 tttcaaaaaa atcgttttta caaatgtacg ggataattta gcaatttaca gatttagttc
    13981 tacgctcact tccaaaccgt tttaataacc aaaaccaatt caagttcatt tttttattaa
    14041 attcttctaa tgttatgcat tcctttctgc taaatccaga ggtaactctg tcgatagtaa
    14101 taaggctttt gcgttagata tgttccgtag gttttcttct acaaagtaat tgacgacagt
    14161 cttcatcctt tttcttgtat gttcctatag aagtatttca tctgttcata ttctaaaaaa
    14221 caatgtgttt tttcgttttt acaatcccaa atactatatc agttgaatga gtgaaatcat
    14281 tgttgtgaaa aatggacaga gcaactgttt ttccaaataa taagatggaa aattttaatt
    14341 tcaatttagt cagaatttgt acggtggaaa aaactttata acatagttac tatgaaccaa
    14401 attgttgtgt ttttttctaa caggagaaaa ccattcgata tcaaatcgtt aatacctgcc
    14461 aagccttcta atttttaccc atacctatct gtctatacta caatttgtcg attttgaatg
    14521 attaagccat cctttaaaag tcaaaagagt aaatcaacaa aaccccaaac ctatattctg
    14581 gttgaggtga aaattccgat aagtttcaac gtaataaaga aaattttttg ttgcaaaata
    14641 atcccttaac tcatttgatt ggagtggttt aacttcagca gaaaaaaaat ttctttaaat
    14701 taccagcgac ggagcagtat atacacggga agccaattcg ttatcaagca aatacaaacc
    14761 gcgtggatcg gtcccgaaaa ggtcgaaccg tttgcacaaa tcgtccacgt ttttttcttc
    14821 ttctccctgt tccttcacaa accagtcgag aaactgcatg gttttaaaat ctttctgagc
    14881 gtatgcctcg gcataaattt cgttgatgga agctgtgata aacaattcgt gttcaaaagc
    14941 agccgacaaa ggagctttaa aatccacaaa attactttcc ggagctgcaa taggtaaaag
    15001 tttgaccttt tcaccattgt ttaaaatata tttcaggaat agctgagcgt gagcatattc
    15061 ttcctgcgcc tgaatggaga accagttggc aaatccgtcc aaatttttgt tggcgtaata
    15121 atttgaaata tccaaataca gataccatga aaaaaattcc ttgttgattt gattgttcaa
    15181 catttcggta attttatcat tcagcataat atttcctccc ttaaattaag tatatgaaat
    15241 ttttaaaatt ggattctctc gatatttgct cccaaagcgt tcaaacgggt atcaatatgc
    15301 tcatagcccc tgtcgatttg gtcaatattg tgaataatac ttgttccatc ggccgcaagt
    15361 gcagctataa gcaaggcaat tcctgcccga atatcaggcg aactcattac cgatgcccgt
    15421 aaacgaagct gatttcccag tccgataacg gttgcccgat gtggatcgca aagaataatc
    15481 tgcgcaccca tgtcgatcag tttatcgaca aaaaacaatc tgctttcaaa cattttttga
    15541 tgaatcaaaa cacttccgtt cgcttttgca gcaattacca gaaaaacgct caacaaatcg
    15601 ggtgtcagtc cgggccacgg cgcatcagcg atagtcatta tggagccatc aataaatgtt
    15661 tcgatggtat agctttcgtg agccggaata taaatatcat catttttctg aagcacttga
    15721 atacccattc gccgaaaact gtcgggaata atgcccaatt catcgtatgc cacatttttt
    15781 atggtaattt cggaaccggt catggctgcc atgccgataa aactgccgac ttctatcata
    15841 tccggcaaaa tagtgtgttc gcagccattt aactgtttca ctccttcaat tgtcaataaa
    15901 ttggacccaa ctccagaaat ctttgcaccc attgctatca acattctgca aagctgttga
    15961 atataaggtt cgcaggcagc attgtaaatt gtagttttac cttcggccag cactgaagcc
    16021 attaaaatat ttgccgttcc ggttaccgag gcttcatcca gaagcatgta agtgcctttc
    16081 agtttttttg ctgatacttc atactgtttt tttgtttcat tgtatcgaaa atcggcaccc
    16141 agcttctgaa tgcccgaaaa atgggtatcc aaacgtcgac gtcctatttt gtctcctccc
    16201 ggttttggaa ccaacgcttt tccaaatcga gccagaagcg ggcccaaaat catcaccgaa
    16261 ccacgcagcg aagaactttt gtcataaaaa tcttccgatt gcaaatagtc catgtttagg
    16321 gagtcggctt taaacgaata agttcccttt tcgatttttg aaatttttac acccaaatct
    16381 tttaacagac ttagtaaatg gcttacgtca agaatatcgg gaatgtttcg aataatgact
    16441 gattcggatg taagcaaggt tgcacaaata acctgcaacg cttcattttt ggctccctgc
    16501 ggtgtaattg atcccgaaag tcgatgaccg ccttctattt gaaatgacga catataattt
    16561 gtggttggat gatgattaaa aaaaggaaac gggtaaaaga aaactatttg tttttctttt
    16621 tatttttctt gttgtgtaat acttctttcg attcaaccaa cttcagaaaa tcttccgaaa
    16681 tatcgagctt ttctttggat aattcacgca aatcgtcgaa aatttttcgg tcatcaacta
    16741 cttccttatt ccatgtaagg aaacattttt tcatctgatt ggctatcagg cgtattaatt
    16801 cgtttttttc ttctccatcg ggataatcgg ctactttttc tatcatttct tccaacgtgc
    16861 ggccatagtg ccgatatcga atatctgaat ttttatatgg aatcttttcg ggacgggtat
    16921 aaagattttc aggccgaaga acctcataag gaaaatcgat atccagttta aaatcggaca
    16981 tgatagccac gtgatcccac aatttatgtt tgaaatcgtt cacatcgcgc agatatggga
    17041 acaaatttcc cataatgttg ataatggttt ttacacaacg ggttcgctct tccctgtctt
    17101 ctatcgtcat ggcataagct atcatctgct gaatattacg cccatattca ggcatgatca
    17161 gttttccctg caaagttgta tattccatgt cgtttataaa taggtgttac ttgattggat
    17221 acaaaattaa tttttgatta tcaatcattt aattatttga taaccatttt ttcactattg
    17281 tactatgcaa aaatagcgat aagtttttga atttatctat tttattgcaa aagaattgaa
    17341 ttttattaaa ctattgaaga gttttttcaa gttgaatatt ttaatgctgc acatcattca
    17401 tttcaaaaac aagagcaatt tctctaaaag tcaaacttcc aaaaagaaaa aaaccgggat
    17461 tttacacccg gtctcaaaag tatgaaatgc tgatattatg gcaggtaaaa gcaatatcat
    17521 acgttgaaag tcttttgaaa aattaatttt cagcatcttg ctcaaattca agattatcta
    17581 tgcaaacata ttttggtgta ttcactccaa aagttccttt atcgctggaa tcgaaaagaa
    17641 aaatcaactg atctactttt ccaagagaag aaaggtcaac tttaacccat tgatggcaaa
    17701 gatattgttt cccgttccgg aaatcggcca aataataatc aaccgcacct gtagcattat
    17761 tattcaaaaa tcccttaatg gtaaccttaa accagtctcc atcgtcaaat tttttactga
    17821 aagccgatcc attgcgcata tcgagatatg cataagttga attgcaaagc atgatacttt
    17881 ttgcttcgta agttcccaat tcatttttcg gacaattcac agtggccgaa tcatcaaagg
    17941 caacagcaaa cttatttgag ccccatgcac ctgaaccggc agccacactg tactgattat
    18001 cgtaaccggc agtaatggta tcggttaaag atgagcaggc aaaccccaac caaaaatcgc
    18061 cccagggcga tgaagtaaaa tcattaagaa aaataaaacc gttggaaacg aatttcttcg
    18121 aaaaatcgga tccattccaa taaccatttt ctcccagctc aacatcctca aaatctacag
    18181 tggtcttgag cttaaagtcc gaatcatttt cgcaagcagt aaaaaaaatc aacaataatg
    18241 tagataaaac aatcaaactt ttctttttca tttttattcc attttaaaat taaacaattc
    18301 gactacaaaa acttgctaaa tttgttaagg aaaattcatg aaaataaaaa agaagaaaaa
    18361 cacatcagat gaaaaaatat attctcaatc agggagatat gtttcaactt tctttgccat
    18421 ttattcccga aagcaaaaaa gcacaaatga tattggcagg tcttctgact tgctcccgta
    18481 tctgaacgcc ttcccatttc ccaaaataga ttctttttgt ggaaaaagtg gccaaagtat
    18541 ggttcagtac gttataaagg agcttacagc agcgggtact gttccggact ttcaccggat
    18601 tcccttttgt ttacttttca gaacaaaaaa taaacaccaa taatcgattg caaaattaca
    18661 aaataaggtc aaaacacaaa atttttttca tcaacttttc acgaaaattt aaattttact
    18721 tttaagaaac tgatagattc atagataagt gaaatttctt tggttattgt ttgataaaat
    18781 gaaatgcttt gattaccgaa atttgtaatt aattttgcaa acaaattaca attcaaatga
    18841 ataaagaaaa aaccacaagc caatatactt ttcaggaagt aaaaccatac aacgaaaaag
    18901 aaggaaaaac agaacaattg gtgcgcatgt tcgacaccat agctgcccag tacgataaat
    18961 ttaacgattt gatgtcgtgg ggttttgcaa aattatggag aaaacgtgca ttaagtacac
    19021 taaagcgctt ccgccccaaa catattcttg atgtggctgc aggaactgcc gatatgtgta
    19081 tcaaagcttt tcggttgctg aatcccgata aagttacagg gatagatatt tctcccaaaa
    19141 tgcttgaaat aggcaaagaa aaaattgaac atgcccaact ttcggataaa atagaactga
    19201 aggtgatgga ctgcgctcaa ctcaattttg acgacaactc ttttgacgcc gtaaccatag
    19261 cttttggggt acgaaatttt gaaaaattgg aagaaagctt gcatcaaatt tttcgtgtgc
    19321 tgaaaccggg aggtcattta ttgatacttg aactgaatga acctcaaaaa ggctggttga
    19381 tgaaaggata ccaactttac atcaaacttt ttgtccacca aacggcaaaa tatctttctt
    19441 ccgaccaaaa cgcctatcgt tacctcacct cttccatgca cgctttcccc aaaggaaagg
    19501 aaatgattga aattcttcaa aacaatggtt ttgaattggt tgaatgcaaa aaatttactt
    19561 ttggtgtttg cagtatgtat ttgatggaaa aaccaaccca ttcaaaaatt gatggttgaa
    19621 aagcacattt caagaatttt ttccgaaaat ttttttggag aacggaaaag tttacatttt
    19681 aattttctct tcctctcaac aaattaatgt cgcctttttt ggaatattta ataccatcgg
    19741 caccaatagc ttttatcaca tagaaataag ctccgacagg cgcttttttc ccacctatgg
    19801 ttccatccca acctttttgt ggatcgttcc aatgataaac cattcttccc catcgattgt
    19861 aaatacgcgc atcaaaacta atcagtgatt tataagccac cctgaattcg tcgttgattc
    19921 catctccatt gggagtaaac acatttggaa cttgaagatc tgaagtgctg actgaaatcg
    19981 ataccgaatc ggatgacgaa caaaaatcat tgtttaccgt cagcttaaca ttataagtgc
    20041 cggcctctgt gaaagtataa cgatgttctt tatcggaacg ggaaacaagc aactgattgt
    20101 ctttataaat atcccatttg taaaaaatgg ttcctgcatt gttgacttcg gcattgaaaa
    20161 gcatttccaa tggagctgaa ccgctcaaag catctgaagt tggacgttca tcttcatttt
    20221 tttctgtacg tgctgtggta gaggttttta tatgacattc cagagaaacg gcagcgtaca
    20281 ttgaagaaat tattccgatt ggctgaatac ccaaagatgg ttcggatgta aattgatcac
    20341 cctgcaaaga aaatgtggtg ttacacaaag gagccggcac agtaatctct gtaaccggaa
    20401 gagtaacgtt ttttgttgtt tcgactgttt tccattcgtt gtcccattcc aaagtttggt
    20461 aactgagcgt gaaatttcta ttgatttttc tacgaatgcc cgataaggta tagtaataca
    20521 gttcaggcac attagcatcg agcaataaag ttaactcgtt gcaccgatta tcgctcgatt
    20581 cgtcagaagc gagcgtatga aaaacgggca aataattttg gtaatcaatc acccagacag
    20641 aagtttgatg accatcgaca tccaaaatat atccgcttgc atcttcagga aatatatagt
    20701 gtaaagtact gtatggtgta gtcgtatcag aaaatctgta ccacttcaca ttatcaccac
    20761 ttcctgtaaa gcgtatttcg gttccgggta aaattccatt aaaaacgaaa atataatcga
    20821 cacctgtaaa attttgatat acagcatatt tatctgttgc aacaagttga gccgaaacgg
    20881 ataaaacaat aagatagaaa caaaaggaaa tcgtaagttt tttcatataa cctgaatgat
    20941 aacgtcagat ttacagtttg gcaaaggtac atttttcttt tcaaaaacaa aaaaaccttt
    21001 gatttattgc ctgctttcct cttttcatta attttttata tttttgcaag gtttagcagc
    21061 cggattcata gaaactttat caattgtcat aaaacaagta tttttctcca aatatccatc
    21121 aaatgaaaaa aaacatcttc tgtttgctga ctgtaatcat attgtcgctt acgggttttt
    21181 caatggaaca aaccaactcc taccctatta aaaaagtgaa tggtattgaa tactatgtgt
    21241 acacggtgca atcgggagat ggtttgtatg ccataagtcg caaattcggc attgcccctg
    21301 aagaaattag caaagttaat cctgaaatac aaaaaggact aaaatcaggg caaaaaattt
    21361 tgattccaat acaaaaaaat gttcaggaaa atgaggatgc aagccagaga ttgaaaaatg
    21421 agctttcatt actgcaagaa aaatcttttg aagaaaatca aaaaaatctt catcagcaaa
    21481 cggacaactc ccttccttca acaactttat tcttacagca taaggttgaa aaaaatcaaa
    21541 cacttttcgg tatcagtaaa aaatacaatg tcagtgaaga ggaaataatc caatataatc
    21601 cacaatctaa aaatattctt agggaaggag acgtcctttt tattccatat aaaacccaaa
    21661 caacaaaaaa tgagacagaa ataaaaggag acttagttga agtgattact ccaagtggaa
    21721 aaaaggaatt gaacgaaaaa cagctgccat caaaagataa acctcagtat atcaaacatt
    21781 tggtaaaacc caatgaaaca ttattttcta tcagcagaac gtataatgtt acaattgatg
    21841 atattttgaa gctaaatccg gaagcctttc ccgtactgaa aatcggcacg gaactaacga
    21901 ttccgacttc tattttgaaa aaagattcga taccatcaac aactctgaat aacaatgaaa
    21961 tggattctgc attgcaagat attgaaaatc ctgaaattac tacggctctt tttcctgaaa
    22021 ataatatcaa aatagctttt ttgttgcctt ttaatcttga cactccttct cagcaaaatg
    22081 caaacggacg ctttagagat ttttatgccg gcagcttact ggcaataaaa aaggccaaag
    22141 agaataatta ttcattggaa attttcactt tcgataccga aaaatcggac gaaaaattga
    22201 aagaaatttt agcaaaacca gaaatgaaaa gtgttgattt gattgttggg cctgcttaca
    22261 gcaatcaaat ttcgttgata agtgattttg caaaagaaaa caaaataaac gttcttattc
    22321 ctttttcttc aaaagtgtat gaaataggca ttaatccgta tctttttcaa ttcaatccca
    22381 acgatactca aatagagata gattttcttg tagataaaat tattcagaaa acttatcgca
    22441 atgctaatat tgtttttgtg aatctaccgg gcatcagcat tcttgatgaa gggaatctgt
    22501 ttatggaaag attgaaaaac agacttttga aagaacgtca taagtttaac gaatttaatt
    22561 ttttttcttc taacaaaaat caaagtattc ctgaagtttt aaaaagaaat caaaaaaatc
    22621 ttctcttttt caatacagat aatttaagtt taattacccc atttttgaaa gaattctatg
    22681 tttatattga tgagttcgat atgatgcttt atcaggaata tggctgggaa aatcaatata
    22741 tagaaaggtt tgacagaatt tatgtgtctc cttttaaacc tgaaatcgat gcttccactc
    22801 ttgaagaata ccaaaaaaca tttcatcaat tctttgaatg ggaagtagaa agtgaaagac
    22861 cacgttatga tttgctcgga tacgatttgt tgaactgttt tattcccatt ttgaaaaatt
    22921 acgaaaatca tcttactaaa aagttgaagg aaataacttt tgatgaagga atacaatcag
    22981 attttcattt caaaaaacaa tcggaatttt ccggttatat caacgacaaa ctttatatgg
    23041 gagaacgaaa agccaaataa tgtcatgcgc aaaatatcca ttttcctttg tattttttta
    23101 ttttctgcca atttattcgc acaacccagc tctgaaaaac cggaaattta ttttggaacc
    23161 actcagggaa tatctgcttc catgatcaat ttttcgccat cagtcaatca gggcatgctg
    23221 cttggttata atggaggatt gatattccgg catatcaccg aaaaaaatgt aggtttacag
    23281 gttgaattga acttttttca acgtggatgg aaagaaaccg attcaattta cagtcgccgg
    23341 ctgaattata ttgaacttcc atttatgacg catatttatt ttggcaataa atcaagattt
    23401 tttttcaata ttggccctaa aatcagttat ttacttacgg aaaatgtttt aaaaaacgaa
    23461 accgtttctt ctgaaaaagt ccaacatgtc aaatccattg aaaatccttt cgattacggc
    23521 atttgtggag gattgggatt tatgttcaga atcagaaaaa acgttttgca gcttgatgct
    23581 cgagccaatt acagcttgag cgatattttt tccaacaata aaacagattt ttttgacagt
    23641 tccaacaata taaatttttc cgttaattta ggctggttgt ggcaggtaaa ataaacagca
    23701 ttaaattcat ggaacttgaa attctctggt ctcgaaaatt tttgtaactt tgtttattca
    23761 aacgaaaatt attttacttt ttatttttta gtcataatag tttaatttac tgagagttaa
    23821 actaaaagtt ggtttatcgg gaaactacaa tgaaattttc tcaatcaaca aaatttcgtt
    23881 gaactaaaaa tgaaaatcaa aaattaaaac gtatatcata caatgtttta tgtgcatgca
    23941 cacataaaaa ttacaaataa cattcaaaaa aatcaattat gaaaactaat tatgaaattc
    24001 ggtatgcttc tcatccacaa gatgcaaaaa attacgacac ctcacgacta aggaaagaat
    24061 ttttggttga aagaattttc accataaatg aggtcaatat ggtttactcc ctgttcgaca
    24121 ggatgttggt gggcggtgcc atgcctgttg ccgaagtact tacattggaa tcgattgatc
    24181 ctttaaaatc cgattttttc cttgaacgga gagaaattgg cattttcaat gttggtggaa
    24241 aaggatgtat cactgttgac gagacaagtt atgaattgga ttataaggaa gctttgtaca
    24301 ttggcagagg gaacaaaaaa gttacttttg aaagcgttga tggttccaat cccgcaaaat
    24361 tttactttaa ttcagctaca gctcataaag cctgccccac cagaaaaatc acaaaagaga
    24421 atgcggtaat tttggaaatg gggagtttag aaggctccaa tcatcggttt atcaacagaa
    24481 tgatggtttc tcaggttctg gaaacctgcc aactgcaaat gggaatgacc gaacttgagc
    24541 cgggaagtgt ttggaatacc atgcctgccc atactcacag tcgtagaatg gaagcctatt
    24601 tttattttga gttgcctgat gatcaagctg tttgtcattt tatgggcgaa cccgaggaaa
    24661 cccgccatat ctggatgaaa aacgatcagg ctgtcatttc tccgcaatgg tccatccatt
    24721 cggctgctgc tacaagcaat tatactttta tttggggaat ggcaggtgaa aatttggatt
    24781 atgatgatca ggatttttac aaaaaaaccg atctgaaata atatttaaat tcgattaata
    24841 tttaattatt tacatgaaaa tcaataaatt atcaacgaat caaaataaca attaaaagac
    24901 atgaatcaat tcgatttaaa aggaaaaatt gctctgatta caggagcatc ctacggcatt
    24961 ggatatgcca ttgcaaaagc ttttcatgct gccggagcag taatagtttt caacgatatc
    25021 aatcaggaaa aagttgacat gggactggcc agttacaaag ccgatggcat tgatgcacat
    25081 ggttacgttt ttgatgttac ggacgaagaa gcagtaacag aagccattgc caaaatcacc
    25141 aaagaagtcg gaatcataga tattctggta aacaatgccg gtatcattaa gcgaattcca
    25201 gcattggaaa tgagcgccaa agattttaaa caagtggtgg atattgattt gaacggtcct
    25261 tttattgttt caaaagccgt cgctccgggt atgatagcca aaggcggcgg aaaaattatc
    25321 aatatctgtt ccatgatgag cgaattgggt cgtgaaaccg tttcggctta tgctgctgca
    25381 aaaggtggac tgaaaatgct tacaaaaaat ttggcatgtg aatggggagc tttcaatatc
    25441 caggtaaacg gaatcgggcc gggttatatc tcaactcctc aaacggctcc tttgcgcgaa
    25501 gaaggccatc ctttcaatac atttattgtt acccgcacac ctgccggacg ttggggaact
    25561 cctgaagatt tgcagggacc tgctgttttt ctggcttcca gcgcttccga ttttgtaaat
    25621 gggcatattc tgtatgtaga tggcggaatt ttggcttatt tgggaaaaca accttcttaa
    25681 ttaaacaaag atttttcaac gagtcaatta aatttccgta caggaaaaat aattgactcc
    25741 aattctttta gatcatgaaa aaaatattcc tctatctggc tactgtactc atggccggtt
    25801 gttcgtcttc aataaaaaca attgaaattt ccaatacggg ttcatttgat cgaaacgacg
    25861 aaataattca aataaatgac aaagaatttt tgcaactgtt tgaagaaagc gacaaatgga
    25921 ttttaaagaa caatcacaat caggaaattg cttaccaatt ggttaaaaat gagaatggaa
    25981 atcttgaaac ttttattttt caggctgctg tccctgcaaa caaaaaagtt gcctatcact
    26041 tgaaaaaagg aaatccttcg ccggtagaac caaagacttt tgctcgcttt gtacctgaga
    26101 gaaaagatga ttttgcatgg gaaaacgact tggcagccta tcgaatgtac gggccggctt
    26161 tggctggaga aaatccaagc aacggagtag atttatggct aaaacgtacc aaagaactca
    26221 ttgttgatac attttacttt aatgaacttc aaaaaggtca atcctaccat attgatcatg
    26281 ggaaaggact ggactgctac aaagttgggc acacattggg ggctggcggc attgctcctt
    26341 tttggaacga ttctctttgg attgggaatt atttcaacag ttacaaagtt cattatgttg
    26401 ggccgcttca atcgaaattc tctttatttt acgattcaat aaaggttgga gacaaattct
    26461 acaaagaaca attaactatt accgtcagtg cgggaacgtt gctaaataaa gccgaagtta
    26521 cctttaccgg ctatcaacag tccttaccat tggcagccgg aatatttctc catgaaaata
    26581 ccggtaaact ctctatgaac caagaagaag gaacagcagc ttattgtgaa gatgccgttt
    26641 cggatgcaaa agttccttca ggcagaaatt acgttggaat ttttgtaccg gctcccatta
    26701 cgaaagcaat gattaaaaac aatcacgctc tgcttatttc cgaatatcca ccgggaaata
    26761 cttttgtcta tttttttggt ggaggttgga acaaatgggg ttttccaacg gacaacgact
    26821 ggataaatgc agtaaatctt tttgcacaaa gttatcgttc tccattagaa gtgttgataa
    26881 aataaaaaat catatcaata caataaacta ttgaccgata aacattttga taaaaagtaa
    26941 aaaaagcaaa cgccagaatt catcctattg tatattttct ttgtcagtct cgacattttt
    27001 cttgaagaaa tttataatag gcaacaagca aaattaagaa caaagttgca tctgacagaa
    27061 agaagaaggt tctctgatac gaaaacctct tttatttcga tcgcatttga attttaatcc
    27121 aaattttgta cttttgtttt atgttaaaat ttttcatcct catccttgtt tctttaaaga
    27181 atgaagacca ataaaatatt ttttttacag caaaagggga aaaaatattt tgttttgctt
    27241 taaaaaccga ataaatgaag taaaaatttg tcgaaaaaga gtggaagtta ttaactatat
    27301 ttatgaatca aagatttgtt attaacattg gacgccaatt gggtagcggc ggtcatttga
    27361 tcgggcaagg gattgcccgc gaattgggaa tatcgtttta tgacaaggaa ctgattcaga
    27421 tcgcctcgca ggaaagtggg ctggcaaaag aatttctgga acagctggac gaaaaaaaag
    27481 gtcagggatt tttcagagga atgtttggac tccgaaattc cattatgatt gatgaatatt
    27541 attttaatga taattatccg agcagtgaaa tgctgtttaa gattcaaagc gatgtgattc
    27601 gcagtttggc cgaaaagcaa tcatgcgttt ttgtaggtcg gtgtgcggat tatgtgctaa
    27661 aggatcatcc aagagcagta aatatattta ttactgccga tttgcctgac agaattgccc
    27721 gcgtatgccg caatcagaaa atgacagaag aaaaagcgat ggactacata aaaaaaatgg
    27781 ataagaaaag agccgattat tacaattatt ataccgataa acaatggggt gcggctgctt
    27841 cctatcattt gtgcatcaat tcgtccgcat tgggaatcga agaaacaatc tcctatatta
    27901 ttgaatttgt tgagaaaaaa ttacatttcg acaaaaaata aatatgcttt ccacaaaaaa
    27961 caagatttaa aagcattcaa atatcattta aagacccttt aaagtagagt tgtttcatga
    28021 ttgaaccggg ttctttccaa cggattggtt tattaaaaat ttgctgtatg tttttttcgg
    28081 cttttgtcag cagaaaaatc atcggaaatt tcaaacaaat tctaaaaaat tccacttatc
    28141 attcggtctt ttccggaaag gtcctttttc aattgaactt ctctgaatcc gttttgggaa
    28201 agcaaatcca gcatgttctt tccacttcga tagtgaattt caaaatatag ttttccgcct
    28261 tttgccaagt ttaatcgagc aaagttggta attgtttcat aaaatttcag aggatgattg
    28321 tcaggcacaa acaatgccaa ggaaggctca taatcggtaa cattgcgttg catttcactt
    28381 ttttcgtttt cgggcacata aggcggattg ctgacaatca aatcccattt tttactgaat
    28441 acaggaggat taaaaatatc tatttctctg aaattgacat ttacacgatt cagtttggca
    28501 ttttcttttg ccactttcaa agcttctggc gaaacatcaa aagcgtccac ttcggccaat
    28561 ggaaagtttt ttttaagact gatagctata catccgcttc cggtgccaat atccagcatt
    28621 ttcaacggtt tgcttcggtc gttttcagaa catatccatt ccaccagttc ttctgtttcg
    28681 ggtcgtggaa ttagtaccga cggattcacg ttgaaactca gtccgcagaa ttcggtagct
    28741 cctaaaacat actgtagcgg ctcatgattt tttagttttt ttaaaaaacc atccaattgt
    28801 ttggctgctt cacaagaaat ttgtgtattt ttgtttagca aaatttctgt tcgtgaaaaa
    28861 ccggtcaatt tttcgagcaa caaatagccg ataaaccgaa tttcactttc aggatagatt
    28921 cctttcagct cggaatgtat gtattgaaga atattttcca tgatacaaag ataaagaata
    28981 tccaaaattt ctcgttagct ttttttgtta caatttcttt tgtttagaac tgattttata
    29041 aagaaccgac atggatattg aaatcaaata tatgcaacga tgcctgcaac tggcttcgct
    29101 tggggatgga tacactgctc caaatccgct tgttggtgcg gtagtggttt ataacgatac
    29161 cattattgga gagggatttc accaacattt tgggcaagca cacgccgagc gcaatgctat
    29221 tttttccgta aaacaacccg aattactgaa agatgctacg ctgtacgtta atttggaacc
    29281 ctgttcgcac tatggcaata cgcctccatg tgcaagttta attgttgaaa aaggtattcc
    29341 ccgcgttgtt atcggcacga ttgaccccaa tcccaaagtt tcgggaaaag gaatccaaat
    29401 actgaaagat gccggcattg aaacgtatgt gggagtactt gaagaaaaat gtagagaact
    29461 aaacaaacgt ttttttacct ttcaggaaca aaaacgaccc ttcattgttt taaaatgggc
    29521 acaaaccatt gatggattta ttgatcgcat tagaaataat gtcagcgaac cgcctttgat
    29581 aatttcaaac gaaatcacca aacaactgac ccacaaaatg cgttctgaaa atcagtctat
    29641 tatggtgagc accaatactg tgctcctcga caacccaagt ttgaccgtga ggaattggat
    29701 aggaaaaaca cccatcagaa ttacgctcga cagacaggga aggattccgg aaaattatca
    29761 tttgctcgat ggtttggttc ctacgttagt ttttaccgaa aggcaacctg caaaacttaa
    29821 caatgtggaa tatattcaaa ttgattttaa aaaagaagga ttacgcggaa ttttaaagga
    29881 aatttttgat cggaatattc attctgttct ggtggaaggt ggagcgcaat tgctgaatag
    29941 ttttatagcc gaaaatttgt gggatgaagc caatgtagaa gttgctccaa tcgaaattgg
    30001 caacggaatt gccgcaccag ttatgacttg tcaaccggcg agcagaaaaa attttgacgg
    30061 acacgaatgg cttcattttt ataacccgaa aaaaacaatt aagtaaatgt cggaaattat
    30121 ttcgattttt tttatagcct tgtatgtcta taccatactg acaacaattt ctgtgctttt
    30181 gctcgaaaac aggaatccgt cgaaatcctt gtcgtggatg ctgattattc tttttctgcc
    30241 ggttattgga ttgattcttt acctgatttt cggacaggat ttgcgcaaac agaaaattat
    30301 ttcacgtaaa agtattcaca gtctcgatca ccgcgacatt tcttcattcg atttcagtaa
    30361 aatcaattcg gatttgctgg atgcccatca aatgaattta atcaaactgt tgtaccgtaa
    30421 tagcgaagca gtaggttata cctacaataa aatagaagtt ttttctgacg ggaaagaagc
    30481 ttttgaatcc atttttcaag ctgtggaaca ggccagggag catattcatt tggagtttta
    30541 cataatcagc gacgacagca ttacaaatcg gctgaaagat ttattgataa aaaaatcgca
    30601 cgaaggagtc agaatcagaa ttatttacga ttttattgga agttttaatc tttctcgaaa
    30661 atttttaaaa actttgacaa aagcaggaat ttacgttcgt ccctttttcc cgcttcgatt
    30721 ccgaatttcg cgcagccgga tcaattaccg caatcatcgc aaattacttg ttgttgacgg
    30781 aaaagtagct tttaccggag gaatgaatat tgccgaccga tacgtgtatg gcaaccgtct
    30841 gggttattgg cgcgatactt ttctcaaaat tcaaggttct gctgttcatg gattacagca
    30901 tctttttctg agcgactggt attttgtgga aaaaaaactg attaccgaca aaaagtatta
    30961 tccggtacca gcgcagtttg acaacaatct ggtacaaatt gtttcgagtg gccccgacac
    31021 cgaatgggaa gccattatgc aaggcatagc caaagccatt atgtcggcaa aaaaatatat
    31081 ttatattcag tcaccctatt acatgccgac ggaactgata gaaggctgtt tgcagttggc
    31141 tgccttgagt ggcatagatg tgaggataat gattccgaca caatccgatt cgccaattgt
    31201 cattgccagt acttccagtt atctgggaaa agtgctgcag gccggagttc gtgtttttta
    31261 ttatcgaaaa ggttttttac atgcaaaaac tgtggtgatg gatgattttg tttccattgt
    31321 tggctcttgt aacattgatt atcgaagttt ttaccaaaat ttcgaagcga atgcttttgt
    31381 atatgaagaa aaaacagccc ttacacttaa agagctgttc ttggaagata tacattactg
    31441 tgaggaaatt acgctggaac agtggaacaa ccgaaaaaaa acaaaaaaac tgatggattc
    31501 cgtttcgcgt cttttcagtc ctcttcttta aaattatttt ttcgcaaatc agaaattaca
    31561 acttacgtgt gattccttgc gtcggaatac tttcttgcct gaaacgattg acagcagtaa
    31621 ccacaaatgt ataatttccc ttaaatttgt ggggtaggat tcgcaaatcg atgcaatttt
    31681 cggttgttcg agctataatg ttgaccggat cattcaagtc gccaacttct tttcccttaa
    31741 aagcatacac cacataatag gcagtttggc agcctccttc ttcattcact tcatcccaca
    31801 acagaaaagc atccttgcca tctctcaaaa cccgcaaatt ttgcggctgc gcagaaggtt
    31861 cgcctaaaat atttctgttt attggacata gtgccggata acgataatag ttcgattgca
    31921 agctgtcatt taacccctgt ctgttttgca gaaaggtttt tgcactgaag aaaacggctc
    31981 cgtccacttg tgaggaattt ctattcattt tgatttgtcg aataatttca tttcctgttg
    32041 tccatgccgg atttttttta tcgcccaaag aggacggaat taatccgata tacagatttt
    32101 ttccgtaaga attttctatc caccatttca acaaaacttc gtagttagca acttttttac
    32161 cgatttccca atacaattgc ggtgtaacat aatctatcgt tccttcggcc agccatttca
    32221 atatatcggc atacaaatcg tcgtaatttt gaatgccagc gcgagttgca gagccacgtg
    32281 gatcgacatt gtcgtttctc catacgccaa aaggacttac gccaaattca acccaaggtt
    32341 tgaggttttt aatggtttgt tgtacttcgg caatagccat atttacattg ttacgtctcc
    32401 agtcggcttt ctgattggga gcaaaacctc ttggaaattt catgaaagtt tcatcatccg
    32461 gaaaatcttc acctttcacc ggataaggat aaaaataatc atcaaaatgt accccatcta
    32521 catcataatg ttctacaata tcttccacca ctttgtttaa aaaattccgt gtttcatcca
    32581 gtccgggatt gaaataatat tttccgccat aacgaacaaa aagctccggt tgtttgtaaa
    32641 acaaatgctc tttcgatagc atcatggaat caccggttgt tgttacccga tagggattta
    32701 gccagacatg aacttccatg cagcgtttgt gagcttcttc tataataaac tgaagcggat
    32761 cgtaaaaagg tgaaggccgc acgccctgtc ggccagtaag ccaatgggac cagggctcca
    32821 gattagaagg ataaaaagcg tcagccatcg gacgaatttg caaaactatg gcgttaatgt
    32881 tgttttcagt cagccgatcg agcatgttca caatttcgat tcgctgctgg gtgggtaaca
    32941 accgattgga gctgggccaa tcgatgttgg caactgttgc tatccacact gccctcattt
    33001 cccgcttcgg ctgagcctgc aaaaacgaga atgcaaagaa taataaaact actgacaaac
    33061 gaaattttct catgatttta ttcgtgaaat tttttaagat ctgtttattt tattttttgt
    33121 gcatttatcc accaaatata ctatcgactg gtatggaatt ccggtgttgg tatttaaacc
    33181 aatttcgcag gtacggctgt tgctgtaacc cactttaaca tgggcttttt ctatttgtgg
    33241 gcgaagtttt ctcaacgcat gagcattcag ttccggcatg gtaaatcctt tgtcgccggc
    33301 aaagccgcaa catccaactt cttcaggtac caaaacctga gtactgcaaa gtttggctat
    33361 ttctacaagc tttggctgca atcccatttt gatggtggag caagtggcat ggatagtaat
    33421 gggctcatcg atagggtaaa aatccaattt atccagtaga aaatcgtgta taaattcaac
    33481 cggttcatat agtgagagtt gtttcatcga cttgcgcatg tgataaagac aaggactttg
    33541 atcacaaaga atcggaattt ttccgttttc ggaagcttca cgtagtgctt tttccaattc
    33601 tgacgatttc tgttcgtcca ccgaaagcaa tcccttactt tcccaaatgc ttccacagca
    33661 cattttttcc atgttttttg gaaaaacaac ttggtagcct gctttatgga gcaaagatac
    33721 ggttttttct acaagtggcg tcagctctgg gtcattttta cttacgccca tcatctgatt
    33781 caaacaactt ggaaagtaaa ctactttcaa tgatgagtca gtcgaggttg aagttgatac
    33841 ttctatttta tttcttcgtt tgggcatggc cggtgtccac aaagggaaaa caccgccact
    33901 ccatttgtgt attccacgag taattgcacc cattgctttg ttacccaata tgtgttgtgc
    33961 agcggaagca atatagagta ccgatttcaa acctgaagcc acccaattga aatgtttggc
    34021 agtccagcgt cccaaaaatt gagccattgt tttgtttttc atgttctcat tgcgaagcac
    34081 atggatatag tcgccaacat tgatcgaaac cggacaagat gttgcacaca gcccatcgcc
    34141 ggcacaactt tgttctccca taaagcgata acttttctcc aattccttca agcgtcggga
    34201 attttcatca gaagttttca atcttttaat ttcgcgttgc acaacaattc gttgccgcga
    34261 agacatggtg aaaccggcac ttacacaatt tacttcgcaa aagccgcatt cgatacattt
    34321 atcgataagc ggatgcacag ccggcatggg tttaatattt tccaaatgac aacgcggatt
    34381 gtcattgaat attactcccg ggttcaggat tccttccgga tcgaaaagtt gtttcacttg
    34441 cagcataatg tcgaaagcct tttctcccca ttcctttttt acaaatggag ccatatttct
    34501 gcccgttccg tgttcggctt tgagcgaacc gtgatatttt tttaccacca tttcaattac
    34561 ttcattcatc attttttcat actgatgaat atattcttcc gtatcaaaac gctgactgat
    34621 aatgaaatga agatttccat cgagtgcatg tccgtaaatg acggcatctt catagtggtt
    34681 ttttataaaa atttcgtgca aatcgtctgt tgcttccgct aaatcttcta tgcagaaagc
    34741 tatatcttct atcaggcaag ttgttcctac tggccgggtg ctacccaccg aaggaaaaat
    34801 tcctgaacgc atgtcccaat attttttgta ttcggattct ttgtcggtaa agctcacagg
    34861 tagaagcgta tgagcatcat tcaggacttt ttccacttgt cgaattcttt cattcaattc
    34921 atttccatct gatgcttcaa tttttataag taaagctgca ctattatccg gcaaagtttt
    34981 tagctcagat atcgtttcaa atttgtcttc tactgaacga atggctttac ggtcgaaaaa
    35041 ttcgacagca ctgacaggaa aagattttaa attttttacg gtttcacaag ctaaatgcat
    35101 gttgtcgaaa tatgccatag cacttgcctt gtacggatag tcggtagttg ttttcattac
    35161 tgcttccgaa agaaaagcga gtgttccttc cgaaccgacc attaaatgtg taataatatc
    35221 gaatgggtcg tcaaaatcga taaaagaatt gatggataaa ccggtaacat tctttatccg
    35281 gtatttcttc tcgcaaatct tctattttgc gaagaaattc gggatgagtt ttagcaaaaa
    35341 ccgctttact ttcttcgtcg cccgtatcca acacactgcc atcgggaaga actattcttg
    35401 tcgaaatcaa cattctataa ctgttttcgc gtattccgca actcattccc gaagcattgt
    35461 taacgattat tcctcccacc atggccgatt taattgaagc agggtcgggt ggaaattttt
    35521 ttctaaaagg ttttaaaatc tgattaacgc gttctcccac aattcccggt tgcaggcgga
    35581 tgaaagaagc atcgggagca agttcgtatc tttcccagtt gctaccggca agaatcagca
    35641 ccgaatcgga aattgcctgt cccgaaaggc tggtaccggc agcacgaaaa gttacgggaa
    35701 gatgatgttt ggttgcagcg cgtaaaagca aactgacttc catttcgctg gcagattgaa
    35761 caactgcttt cggtatcaaa cgatagaagc cggcatccgt gccccatgcg agtaaacgta
    35821 attcgtcggt gtaaattcgt ttttcgggga taaaactttt aatttcggat aaaaaggagt
    35881 ttatcatttc atttgatcag tgatacaaat aatatttttt tgaaagttga taataggact
    35941 gaatatcttt gtcccaatag tgctgaatgt cggataccga attgttaact tgaaacagtt
    36001 ttctcatttg atcggttccg cttaccttat caaacatgga aaatctttct ttgttggcat
    36061 tgtcgaatac tttcttatcc ggatacaaac gtgccaactc ctgcataata taaaattgaa
    36121 ccaaagacag atgggctttt ttgaaatcga caatgtgaat ctgcactccc tgacaatttt
    36181 ctccttttaa agttccataa aatggagtga aaaagatggg tcgaaaaatg aagcccggca
    36241 aatgtaaacg attcatggca tcggcaaatt cctgtgcttt tatccagggc gctgcaacaa
    36301 cttgaaatgg aagggtgtag ccaacgccta ccgatacgta acccagctca cccaaaattc
    36361 cggtaaccgg atagtagtaa gctgaatgtt tatgcggaat gtgaggtgaa gcaatgaccc
    36421 attccaatcc ggtgtcttcc catttcatac ttcttttcca gttcttcatt ttgacaaccg
    36481 tcagtttgca tttctttttc agtgcgtttt cttcatttaa cattagggcc agttctccac
    36541 aagtttgacc gtaaatataa ggaattttaa actgactgac aaaagaaata aaaggttctt
    36601 ccgtcaggtt gccttcaatt ttttctccac cgagtggatt gggacgatcg agcacaataa
    36661 attctatatc gttttcggca gcagcttcca tagctaaacc catggtgctg atataggtat
    36721 aggaacgaca acccacatct tgaatgtcat aaaccagcac atcaattcct tttaacattt
    36781 cagaagttgg tttgcgtgta gttccataaa gcgaatacac aggtaaacct gttttctcat
    36841 ctgtggcatt cacaacttta tcgcctgcat aaacatcacc tcttacgccg tgttcaggcc
    36901 cgtacaaggc taccagtttt acgttgcgtg cttcatataa aatatcgatg gtagatttta
    36961 aatttccatc aacacctgtc ggattggtaa tcaaacctac tcttttgcct tccaacaatt
    37021 tgaaattatc tgctctgagt acttcaatcc ctgtcattac gcgtttggcg tataaaggaa
    37081 aggcaaataa aaccgaaaaa ataaaaataa ttagtttttg catgattcat attttttaaa
    37141 ttgacgcaaa cgtttgcatt gaaaaacaaa tgtaattaaa aaaattgaat tgaataaagg
    37201 cagttgaaat attttttagt tcattttttg tcagataatt gtatgccaag tatttaattt
    37261 ttttcttagg aaaaatttct cgtcattgtt tatataaatt cagcttagat tcaaaactaa
    37321 ttaccacttt ttttctttat ttaatcccaa aaaatcttaa agaatcgatt taaagtagta
    37381 attttgcagc tcaaagaaat ttcgataaaa atttgatatt atggcagaaa aaaaattaaa
    37441 tttattagct gattttcctc ctgtctccac gcaggaatgg ctggataaaa tcattgcaga
    37501 cctcaaggga gccgattttg agaaaaaaat gatctggaga accaatgaag gtttcaatgt
    37561 gagacccttt taccgcgagg aagatgtgga tgatctgaaa atcaccgaat tgttgcccgg
    37621 agctttccct tatgtgaggg ggacaaaaag cgataatgaa tggtttgtac ggcaggacat
    37681 aaaagttgaa aacgcacagg aagccaatgc taaagccctg tatattctaa atcgaggggt
    37741 gaactccatt ggatttcagc tgaaaaaagc agatctatct aaagaatttg ttgccacttt
    37801 gctcaaggat attgcagctg actgtgtgga gttgaatttc acgatttgca tcagcaaagc
    37861 tgccgaactt gccgatattc tctccgagta tttcattgct caaaattatg aggtcaagaa
    37921 attgcagggt tctatcaatt tcgacccgat taatcgcatg ttgctggcag gcaaaaaatt
    37981 gacgaaagaa gagattgccg aaaaggctaa agctgttgtt caggcaataa gcagattgcc
    38041 tttttatcgt gtaattggcg tgaatgccac cactctctcc aacgcaggtt ctaccattac
    38101 ccaggaactc ggttacgctt tggcatgggg aaacgaatac ctttccatgt tgatagacgc
    38161 aggagtggat aaagcggttg ccgccaaaaa aataaaattc aattttggta tcggcagcaa
    38221 ctattttatg gaaattgcaa aattccgtgc tgcacgcatg ttgtgggcat taattgttga
    38281 tgcttatcaa cctccctgtc ctcgtcccga ctgtcccaat acagtaaatg gaatttgcaa
    38341 atgtgcagca aaaatgcgta tccacgccga aacgaccatt ttcaatcaaa ccatttacga
    38401 tgcttatgtc aacatgcttc gtacacaaac cgaagccatg agtgcaacta ttggaggtgt
    38461 tgattctttg tcggtgctcg gttatgacac tgctttcaaa ccttccgacg aattttcgga
    38521 aagaattgcc cgcaatcagc aattactcct caaagaagaa gctcatttca gtgaagttgt
    38581 tgatccggca gccggttcct attatatcga aactttaact gccgaaatag ccaatcaggc
    38641 ttggaaactt ttcctcaatg tggaaaacga aggcggattc cttgctgccg ttcaaaacgg
    38701 aaaggttcaa cagaccatca aggctacagc caatgaacgt ttaaaagcta tctcgaaccg
    38761 gaaagaaatt ttggtaggca ccaatcaata tccaaattta ttggaaactg ctgccgaaaa
    38821 aatcgacaag gaattgcaac agtgtggttg ctgttcgcat gaagaaggaa cagaaaagct
    38881 tcttccgttg cgtggtgctg tggaatttga aaacttacgc ttagcggtcg aaaaaacgtc
    38941 caagcgtcca aaagtgttta tgttgaccgt tggaaattta gccatgcgct cggcaagagc
    39001 tcaattttcg ggcaatttct ttggatgtgc cggatacgaa attatcgaca atctcggatt
    39061 ctcttctgtg gaagaaggcg taaaagcagc gttggatgca aaagccgaaa ttgttgtgct
    39121 ttgctccagc gacgaagaat atgccacatt agcccccgaa gcttacaaat tgttgaagga
    39181 caaaacgatt tttgttgtgg caggcgctcc cgcttgtatg gaagatttga aaaaggaagg
    39241 gattgagaac tttatcagca taaaatccaa tgtgttggaa accttgaagg atttcaacaa
    39301 aaaactgggt ctgtaaagtt tttgtatatc attttcattt ataaaaaata aattctccag
    39361 tcaaaaaatt ttttctatcc aacatcttca aaatgaaaga aaaaattaaa aacggattgc
    39421 tggctaccag gccctggtct tttcctgctt caaccatgcc tgcattgttg accatttcgt
    39481 atgtcttttt tctgaaaaat gagcttggaa gcgaggttaa ctggtggttt ggggtgttgg
    39541 cacttttggg tgccgccatt ttccaagcca gcggaaatct gattggcgat tatttcgact
    39601 atcaacacca tgtggaccgt aaagagacgt ttggttcaag ccgaatgttg gtcgatcagg
    39661 tttttcaacc caaaacgatt ttgcattttg gcattgtttt gctggcggta ggtgttgtgc
    39721 tgggactttt tctttgttac cgaacgggca tcgatttgtt gtggatcgga gcgattggcg
    39781 caatggcaac ttatttctat tacaaattga aattcgctgc gctaggtgat ttgactatat
    39841 ttatcatcta cgggccgctg atcgggttgg gtacagctta cgtcatgacc ggacaactca
    39901 tgtggtttgc agtggtgttg aatatacctg ttgccttctt ggttgtcaac attttacatg
    39961 ccaacaacac aagagacatt cgcgacgacg gaaaagccaa catcaaaaca caagccatgc
    40021 tgttgggtat caaaggtgca aaaatacagt atgttgtttt gagtattgga gcttatgtgg
    40081 gtgtggtgtt gatgattatt ttcaaaatga ttcatccggt tgctttgctg atgttgcttt
    40141 cgttgccttt ggctgtgaaa aacatcaaaa aaatgatgac cgcagaagtg gaaaaacccg
    40201 aaaagatttt tgatttggat gcacaaactg cacagttggt acttgtattc agcttgttgt
    40261 tctctatttc aaattttatt gctgcatggt tatgaaaaga attattattc ccatactgat
    40321 tgctatcatt ttttggtttg tcatgttttc tccctggacc aaagaatatg tcaacttctg
    40381 gctgaccatg acggctgccg gcataacgct gactttgatg agtgcttttc tcggaaaaga
    40441 tttcaaaaaa caattttcgc tttcgctgaa agatgtactc attggtctgg gatcggctgt
    40501 tgttttatat gtggtgtttt tcttgggcga tttcttttca aaattgctgt tcgattttgc
    40561 aaaagatcag gttgggcaga tttacatgat gaaagaagga gaaaatcctt tgttgttgag
    40621 tttattgctc attcttttga ttggcccggc agaggaaatt ttttggagag gatacgttca
    40681 gcgaatgctg gaaccaaaat ttggttcatg gatggcattg atagttacaa cgctgattta
    40741 tactttggta cacatttggt cgtttaactt catgctgatt atgtctgcca tggtttgcgg
    40801 tgcattttgg ggattgcttt acaaatacaa taaaaatttg gtaaccttga ttgtttcaca
    40861 tgcggtgtgg gatgtttccg tttttatatt attccctatc gtgtgaaaaa tgaaattatc
    40921 aacgtccaaa actgaaaaat tacaataact tgtcctatta gattttttat tatcgattaa
    40981 tgattttatt ttagaaaaaa gtaaacattg aaatacagta ttttcgactg accattgaat
    41041 tttcgtcgtc ggaaagtctg attcaaaaaa caaaaaaaat atgaaaccga attttaacaa
    41101 cattgatgtc agcaacatta cttcaagtgg agctgccatg cttcagactg aagaaaattg
    41161 gctaacgccc gaacaaattc ccgttaagcc tttttatgag aaaaacgact ggaaaggaat
    41221 ggaacattta cattatgcag ccggaattcc tccttttttg agaggtcctt acagcagcat
    41281 gtatgtaacg cgtccgtgga ctattcgcca atatgcaggt ttttctacag ctgaagaatc
    41341 caatgctttt tatcgtcgta atttggctgc aggtcaaaaa gggctttccg tagcattcga
    41401 cttgccaacg catcgcggtt acgatgccga ccatgaaaga gttgtgggcg atgtggggaa
    41461 agcaggcgta tccatttgtt ccgtagagga tatgaaaatt ctctttgacg gaattccctt
    41521 gaataaaatg tccgtttcca tgaccatgaa cggcgccgta ctgcccattc ttgctttcta
    41581 tattgtggca gggctcgaac agggagctaa actcgaagaa ctttccggaa ccatccagaa
    41641 cgatattttg aaggagttta tggtgcgaaa cacctatatt tatccgccac aattttccat
    41701 gagaattatt gccgatattt tcaaatatac ttcgcaaaat atgcccaagt tcaattccat
    41761 ctccatttcc ggttaccaca tgcaggaagc aggtgctact gccgatttgg aactggccta
    41821 tacacttgcc gatggtttgg aatatttgcg taccggtatc aatgcagggc tcgacatcga
    41881 tgcttttgca cctcgtctat ctttcttctg gggtatcgga atgaatcatt ttatggaaat
    41941 tgccaaaatg cgtgcggcca gacttctctg ggctaaaatc gtgaaacagt ttcatcctaa
    42001 aaatccaaag tctatggcac ttcgtacgca ctcccaaact tcgggatggt cacttacaga
    42061 acaggaccct ttcaacaatg taggaagaac ctgtatcgaa gccatggcag cagcgctggg
    42121 tcatacacaa tcgctgcata ccaacgcttt ggatgaagct attgccttgc ctaccgactt
    42181 ttcggcacgt attgcccgca acacgcagat ttatcttcag gaagaaacca atatttgcag
    42241 acaaattgac ccctgggcag gttcttatta tgtagaatcc ttgaccaaag aattggccga
    42301 aaaagcatgg gacttgatac aggaaatcga aaatctggga ggaatggcca aagccattga
    42361 aaccggtatc ccgaaaatga aaattgaaga agctgctgcc cgtacacaag cacgcatcga
    42421 ttccgggaaa caaataattg ttggagtcaa ccgatatacg ctggaaaaag aagaacccat
    42481 cgaaattctc gaaatcgaca atacggcagt acgcatgcaa cagattgaaa gactgaaaca
    42541 gctccgagct tccagaaaca acgaagaagt gaaaaaagct ttggatgcca ttacagaatg
    42601 tgccagaacc ggtaacggca acttgctgga gcttgcagta gaagcagcac gtgtcagagc
    42661 aacactgggc gaaatttccg atgcctgcga aaaagtggca ggtcgttata aagcaatcat
    42721 aagaactatt tcaaacgtgt attcatcaga aacaaaaaac gatgcaaatt tccagcgggc
    42781 ttcgcagctt actgaagaat ttgccaaaaa ggaaggccgt cgtccacgta ttatgattgc
    42841 caaaatgggg caagatggac acgaccgcgg tgcaaaagtt gtagctacgg gctatgcaga
    42901 ttgtggtttt gatgtggata tggggccgct gttccaaact ccggcagaag ccgccaagca
    42961 agctgtggaa aacgatgttc atgtagtggg tgtttcttca cttgctgccg ggcataaaac
    43021 cttgattcca caacttattg aagaattgaa gaggctggga cgtgaagata ttctggttgt
    43081 tgcgggtgga gttattcctc cacaagacta cgacttcctt tataaagccg gagtagccgc
    43141 tatttttgga cccggaactc ccgttaccaa agctgcctgc accatcatgg aaatgctgtt
    43201 ggctgattaa atgaactttt tactgaaaca aaaaacaggc ttccccaaaa gttgcctgtt
    43261 ttttttttat cccagattac tcattagtca atttaaaggg gaaatcaaac ttagaagaaa
    43321 tctaccatgg gccggaaagc cagggtcgtc attaaaacct tttttagtac atgacaaaaa
    43381 ttgagacatt tgtttatgtg taaaacagga catataagtc aagctcttgg ggttgacctt
    43441 gtccggtgag ccg
//
LOCUS       DAOSCH010000006        41432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_713_length_41432_cov_7.383087, whole genome shotgun sequence.
ACCESSION   DAOSCH010000006 DAOSCH010000000
VERSION     DAOSCH010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 41432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41432
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_713_length_41432_cov_7.383087"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..1064)
                     /gene="atpG"
                     /locus_tag="PLZ54_01125"
     CDS             complement(204..1064)
                     /gene="atpG"
                     /locus_tag="PLZ54_01125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOJ65575.1"
                     /translation="MSSLKELKSRIQSVKSTQKITSAMKMVSSVKLLKAEKAVYNFLT
                     YRETLTDILRNYLSSQIVDRSPFREIRHVNHVGIVVFSSNSSLCGNYNSNIIKRLEQS
                     ITTHRDVRKEDILIFPIGKKVEDFCKEKKLAIAGEFQNVAAHPNIENTTQIADTLIQL
                     FLQHKIDRIKVIYFHYVNKTTQIMKAETFLPIEINYRKKGEFRNDYLIEPSIEEIEQQ
                     LIPLALHYHIYTAALEAYASECAARTLSMQAATENAENLLQQLSLEYNKMRQESITNE
                     ILDLIGGSLQ"
     gene            complement(1112..2704)
                     /gene="atpA"
                     /locus_tag="PLZ54_01130"
     CDS             complement(1112..2704)
                     /gene="atpA"
                     /locus_tag="PLZ54_01130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOJ65576.1"
                     /translation="MSDNKTIKVSEISELIKKQLQQIDIESYYEETGKVLEVGDGVVR
                     IYGLTNAEANELLEFENGVKAIAMNLEEDNVGAVLLGPTHLIKEGFTVKRTGRVASIK
                     VSEKMLGRVINPLGEPIDGKGKIDGEFFEMPLERKAPGVIFRQPVEEPLQTGLKAIDS
                     MIPIGRGQRELIIGDRQTGKTSIAVDTIINQLDNFKAGDPVYCIYVATGQKGSTVATL
                     FNTLKDFGAIPYTILVVATAADSAALRYLSPYAGAAIGEYFRDTGRHALVVYDDLSKQ
                     AIAYRELSLILRRPPGREAYPGDIFYLHSRLLERAAKIINQQEVAKQMNDLPECLKGK
                     VKPGGSLTALPIIETQAGDISAYIPTNVISITDGQIFLDTDLFNEGNRPSIDVGISVS
                     RVGGNAQIKPMKKVAGTLKTDQAQFRELEAFTKFGSEMDAVTAMTIDKGQKNTQLLIQ
                     SLHRPLSVEKQVALLYCGTHGLLANIPLHKVKEFEENFINLLDYSYKEKILNEIKKGN
                     FDEKVTSSIEEAAEILKKQMNY"
     gene            complement(2746..3297)
                     /locus_tag="PLZ54_01135"
     CDS             complement(2746..3297)
                     /locus_tag="PLZ54_01135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HOJ65577.1"
                     /translation="MQTGLIASRYAKALYEFASEMNAQEIVFANFQTLEKSFSANAEL
                     AQALKNPLLTRETKKQLIQFACGNKSDFILERFMDVLLQNRREYLLRNIGLKYSDYYY
                     ENKNLALVKLTTASPMDAETEKKLISQIKTYIKKELKVEKIIAPEIIGGFIIEIGNRR
                     WDGSLATQLKKIQKELNENILLT"
     gene            complement(3371..3877)
                     /gene="atpF"
                     /locus_tag="PLZ54_01140"
     CDS             complement(3371..3877)
                     /gene="atpF"
                     /locus_tag="PLZ54_01140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOJ65578.1"
                     /translation="MSLLMPDFGLFFWMMLSFGIVVFILVKFGFPAILQMIEKRRSYI
                     EESLLEAENARKELEEIKTEAKKIQDEAFLNRKKILDETTEIKNALIKEAKEKAFIEA
                     EKIMEEAKKQIRYEKMMALQEIKQQVAEISIAVSEKLLQSKLNDKPAQMEIINRMLEK
                     MDIGKFGN"
     gene            complement(3988..4236)
                     /gene="atpE"
                     /locus_tag="PLZ54_01145"
     CDS             complement(3988..4236)
                     /gene="atpE"
                     /locus_tag="PLZ54_01145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOJ65579.1"
                     /translation="MLLSILLQVAASDALAKFGAALGAGLAAIAAGIGIGKIGGSAME
                     SIARQPEMSSDIRMNMIIAAALIEGVALFAIVVCGFIL"
     gene            complement(4323..5288)
                     /gene="atpB"
                     /locus_tag="PLZ54_01150"
     CDS             complement(4323..5288)
                     /gene="atpB"
                     /locus_tag="PLZ54_01150"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOJ65580.1"
                     /translation="MSDSYEWYITTWKSKSISIPLPVILHSNNSGWHVFMSSKFEEQP
                     VYKGFYIAKEGKYKDKIVELDENGKEIRPLDISLTKTATALIFNSVLLIILIMSIAHY
                     YKKDGIFPKKGFVILMEMLIVDIYEGVIKPSIGNDYRRYAPYLLTVFFFIFLNNLMGL
                     IPFFPFGATVTGNIAITFTLALFTMLIVNFSGTKEYWKEIFWPEVPAWLKVPVPLMPA
                     VEFIGIFTKPFALMIRLFANIVGGHSIVGGLVCLVFVTVKLGTTINVTMSALSVIFTV
                     FIDLIELLIAYIQAYIFTMLSAVFIGLARVEPSHKQAAKEVVKQV"
     gene            complement(5411..5806)
                     /locus_tag="PLZ54_01155"
     CDS             complement(5411..5806)
                     /locus_tag="PLZ54_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65581.1"
                     /translation="MKNNLLFSLVFFSLLTLLLGLFFWLLLKKFSSTTIENYFLLPLL
                     FYFSGLTLMLVLQKFSYISSTKKIYLFLLLKLSKMILWGIVAFVYLLVFRTERRMFGL
                     LYGAYYLIYLIFETACFYMNEKRQVRQSK"
     gene            complement(5796..6050)
                     /gene="atpC"
                     /locus_tag="PLZ54_01160"
     CDS             complement(5796..6050)
                     /gene="atpC"
                     /locus_tag="PLZ54_01160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HOJ65582.1"
                     /translation="MNLKIVLPNKLIYDGEISSVTVPGKGGVFTILSHHAPLIAILGK
                     GNVTFRFKNGVNNVFINGGLIELNNDKVVICAEQIPENEK"
     gene            complement(6047..7594)
                     /gene="atpD"
                     /locus_tag="PLZ54_01165"
     CDS             complement(6047..7594)
                     /gene="atpD"
                     /locus_tag="PLZ54_01165"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOJ65583.1"
                     /translation="MLSIQGKVWQIIGPVVDVFFPLEEKNEFELPPINNALSISLSNG
                     KNLILEVQQHIGENIVRTIALDSTDGLSRGLPVSDLGSPISIPVGNQVKGRLMNVIGE
                     SIDGLSPLGRVESAPIHKEPPKFDELANNDEILYTGIKVVDLLAPYVKGGKIGLFGGA
                     GVGKTVIIMELINNIAKWHKGFSVFAGVGERTREGNDLLREMIEAGVIRYGEEFNKAM
                     QEGKWDLSKVDYEQLKKSQATLVFGQMNEPPGARYSVALSGLTVAESFRDSDESENHD
                     ILLFIDNIFRFTQAGSEVSALLGRLPSAVGYQPTLATEMGLLQERITSTKSGSITSVQ
                     AIYVPADDLTDPAPATTFSHLDATTVLSRQIASLGIFPAVDPLDSTSRILDPLIVGQE
                     HYETAQRVKQILQRNKELQDIIAILGIEELSEEDRLTVQRARKVQRFLSQPFFMAEHF
                     TGVPGVMVPIEETIHGFKLILDGMLDDLPEMAFLNVGTIKDVLNKGHALLREAHNLNK
                     TFKIEDQ"
     gene            complement(7733..9520)
                     /gene="lepA"
                     /locus_tag="PLZ54_01170"
     CDS             complement(7733..9520)
                     /gene="lepA"
                     /locus_tag="PLZ54_01170"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOJ65584.1"
                     /translation="MNNIRNFCIIAHIDHGKSTLADRLLEYTKTVVGKDLQEQVLDNM
                     ELERERGITIKSHAIQMNYQYQSIDYILNLIDTPGHVDFSYEVSRSIAASEGALLVVD
                     ATQGIQAQTISNLYMALDHNLTIIPVINKIDMDSARPEEVEDEIVELLGCSREEIIRA
                     SAKTGQGVEEILNAIVERIPVPQGDPEAPLQCLIFDSIFNPFRGIIAYFKIVNGTLNK
                     GDWVKFVATGKDYYAEEIGVLKLEPQPVQTLSAGNVGYIISGIKTSKEVKVGDTITHV
                     KRPCQKAIAGFEEVKPMVFAGVYPIDTEDFEDLRASIEKLQLNDASLTFEPESSTALG
                     FGFRCGFLGMLHMEIIQERLEREFNINVITTVPNVSYKIYTKKGELIEVHNPSEMPDP
                     TLIDRIEEPYIRASIITPMEYIGPIMTLCLGKRGELIKQEYISGNRIELIYDLPLSEI
                     VFDFYDKLKSISKGYASFDYHMNGYRASKLVKLDILLNGEPVDALSSLIYADNAYSLG
                     RRMCEKLKELIPRQQFEIAIQAAIGAKIIARETIKPVRKDVTAKCYGGDISRKRKLLE
                     KQKKGKKRMKQIGSVEVPQKAFLAVLKLD"
     gene            complement(9725..11548)
                     /locus_tag="PLZ54_01175"
     CDS             complement(9725..11548)
                     /locus_tag="PLZ54_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOJ65585.1"
                     /translation="MLQKIIQFFKIYLLILFVFVLQKPFFILYNHQIYSNLSFQDLIL
                     IILHGIKLDASMSAYLTILPGIFLIVSLWAKPSVHEYFQKIYFGLISFIISVVFVVDA
                     ALYPFWGFRLDSTVLFYMKWPKEALASIDIWMIVVGLLAIILIALTLYAIFYFLLIKI
                     NKESKQVKNKLSTACLLFLFTCLLFIPIRGGFNVSTMNVSKVYFSPKNELNHAAINPM
                     FNFLESAFKTQNFDKQYRFMSEEEAQKEFNNLIDQPVQEELPKLFTSPKPNVIFVVVE
                     SFMSNNLEPLGGFPGIAVRMNEFCDEGILFTNFYANSFRTDRGLISIFSGYPAQPTTS
                     IMKYPKKSQSLPSIPKTLKTAGYDLQYYYGGDVDFTNMRAYLIAQGIDKMVVDKDFPL
                     KDRTTKWGAHDHIVFQRLTNDLKKEQKEPFMKILQTLSSHEPFDVPFYKNSDPYLNSV
                     QYTDSCLGAFVDEFRKTKYWENTVLVLVPDHAMHYPKNILLSSVERYQIPLLIIGGAV
                     KSPMKIDTYASQIDIAATLLYQLELPHQDFTFSKNILNPASPHFAFYTFPNGFGFLTP
                     ENHYVFDCSTNKVLWNWGQPNANKKKGEAYLQMLYNDLSKR"
     gene            12119..14173
                     /gene="htpG"
                     /locus_tag="PLZ54_01180"
     CDS             12119..14173
                     /gene="htpG"
                     /locus_tag="PLZ54_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOJ65586.1"
                     /translation="MTKGTIGVTSENIFPVIKKFMYSDHEIFLRELVSNAVDATQKLK
                     TLASVGEFKGELGDLKIRISVDKKKKTITISDRGIGMTAEEIDQFINQIAFSSAEQFL
                     EKYKNDAQSIIGHFGLGFYSAFMVSKKVEIFTLSYKEGAVAQHWSCNGDPEYVLEDTI
                     KSDRGTDVVLHLDDDNLEFLEESKIRELLLKYCRFLPVEIAFGKKKEWKDGKYVETDE
                     DEIINNTQPAWIRKPSELTEEDYLNFYRELFPYNEDPLFYIHLNVDYPFHLTGILYFP
                     KIKNNIDIQRNKIQLYCNQVFVTDHVENIVPEYLTLLHGVIDSPDIPLNVSRSFLQND
                     SNVRKISNHITKKVADRLQEIFKNDRNQFEQKWDDLKLFIQYGMLTDEKFYERAEKFA
                     LFKNVDGKYFTYEEYKTLIGENQKDKNGDLIYLYATNAEEQYSYIQHAKDKGYDVLLM
                     DGQLDIHLIGHLEQKWEKTRFVRVDSDVIEKLIQKEDTANVSLSEAQREALTAIFNSQ
                     LPPMEKTNFIITFEQLGPNVNPVLITQNEFMRRMKEMSAMQGGMMNFYGEMPDSYNLV
                     VNTTHPLIEKLLKDEETACAEELAPIEAELLELSNKLQQLKENHKNKKEEEIPAEEKE
                     ELTTLEKRIDELKEKRKSVLAAFASNYKTVRQLIDLALLGNNMLKGEALANFVKRSID
                     LM"
     gene            14315..15022
                     /locus_tag="PLZ54_01185"
     CDS             14315..15022
                     /locus_tag="PLZ54_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5020 family protein"
                     /protein_id="HOJ65587.1"
                     /translation="MRKKTTLFFAFLPLAAMAQNFQLHYDFGQGRHYVTTTFEMFKPD
                     DWGNTFFFVDYDFNMDQDHNASLSYMELARCFSLGKTSPFSVQVEYNGGLFAMEGSAY
                     PIQNAFLIGLDYGWHNNNFDRFLNFKVLYKNIVGKHPLSFQLTGVWDLSFYNKRISVC
                     GFADFWWEDNLNFTDAAGKNLDSPVLTRYVFISEPQFWYNITPHLSAGSEIEIAANFS
                     SVYGLKICPTLGMKWNF"
     gene            15176..16153
                     /locus_tag="PLZ54_01190"
     CDS             15176..16153
                     /locus_tag="PLZ54_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOJ65588.1"
                     /translation="MKNFLVFIVMAGFLVSCHSATQKSANEEKVINIPVFDSDSAFSF
                     VQRQVDFGPRVPNTPAHDACANFFVATLKSFGAQVTTQQMDLRTFDGTLLHAVNIIAS
                     YLPESETRILLFAHWDSRPWADNDPNPKNREKPVLGANDGASGTGVLLEIARQLGKNP
                     ANVGVDIIFFDAEDWGASNDDQTSNTENSWCLGAQYWAKHPHKQGYHARYGILLDMVG
                     APQATFYREQYSEYYAAHVVDKVWSQAQNLGFGQYFINQKGGGIMDDHVFVNQIAGIP
                     SINIIHFDPNTSSGFGNYWHTTHDDMSNIDKNTLFAVGTTLMNVIYNEK"
     gene            16157..17239
                     /gene="proB"
                     /locus_tag="PLZ54_01195"
     CDS             16157..17239
                     /gene="proB"
                     /locus_tag="PLZ54_01195"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOJ65589.1"
                     /translation="MEFRFKRIAIKIGSNVLTKSDGTLDTESMSNLTNQIATLYHQGI
                     EIVVISSGAVASGRSILGLNKKMDIVDQRQLYSTVGQAKLISLYWDLFAKHHITIGQV
                     LTTKQNFDSRRNYLNQRNCMRVMLENKVLPIVNENDAVSVSELMFTDNDELSGLIASM
                     MGMEALFILSNVDGIYRGSPSDPKAEVIREINRGQDISDFIQTTKSSFGRGGMQTKVS
                     IARKIADQGIEVFIANGRKENILVRLIDAADDVVCTHFIGYRGEVSSVKKWIAHSEDF
                     AKGEIKINTNAVKALMGDKAVSLLPVGVTEIIGDFEKDDIVKIVDENGQSIGVGKVRY
                     DSKKARELIGKKYQKPLVHYDYLYLE"
     gene            17409..19673
                     /locus_tag="PLZ54_01200"
     CDS             17409..19673
                     /locus_tag="PLZ54_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOJ65590.1"
                     /translation="MRNIKIRLKIETIAVALLLTGCNTTKFVPEGEYLLDKVNINIDT
                     RNVKKIELKEYLRQTPNSSVFGLFRTQLGIYSLAGKDTSKWINKTLMRIGEEPVIYNP
                     ALTEISSQQLQLFMHNKGFLRAKVENKVTLKDKKAEVEYQILAHQPYRIRNYSVQLNH
                     PELQAIAADTSRSLIRPGMLFDVDMLDAERERITSRFRQTGYYNFSKDFIVYSADSIE
                     KTHQVDLSMQLRDYLNDKNDSLSQIIFRKFYVRNVYFEINPTDLPSLNQPMEKDTVWF
                     RNFAMITPHKQIIKIDALVQNTYIDPQALFNDANIEKTYSGLNSLGAIKFVNIGFEQV
                     DTGLLDCHISIIPGKAISLSAEVEATYTDGYWGGATNLNLVNRNIFKGAETLSLQVHG
                     AYELQEKVWAQEWGGQIGLQFPRVVLPFASYDFKRNLRANTEFSGSYYYQFRPGEFRT
                     SNLGAGMRYFLNRRQYTHIFDLFEVNYVYFPEIFPAFRDSFLTTGIFNSYNYENHFIM
                     RMGYSGSSTNFNANQPLRNYRTMRYSIETAGNILYGLDHLLGSRPAEDGNYRLLNIAY
                     AQYFKTEYNLTYHQIFDENNRFVYHAGLGIAIPYGNADAVPYEKRFFSGGANGVRGWS
                     ESTLGPGVYKRINTQRRDFNQTGDIKLDMNMEYRGKLFWLLESAVFMDAGNIWTIKDY
                     DTQQGGLFKWDSFMNQIAVAYGVGLRFDFSFIVARLDMGVKLYDPVLKRSDRWRVSPQ
                     WNDVAFHIAIGYPF"
     gene            complement(19705..20358)
                     /locus_tag="PLZ54_01205"
     CDS             complement(19705..20358)
                     /locus_tag="PLZ54_01205"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOJ65591.1"
                     /translation="MNKIPISKIKLYPDKTNRKQPSLIRSFSSFQHECGCDEAGRGCL
                     AGPVVAAAVILPPDFICPQLNDSKQLSPLLREKFRKIIEKEALAWSVASVDNREIDRI
                     NILNASILAMHKAIDGLQIKPQFIAVDGNRFKPYADIPFQTIVKGDSKYLSIAAASVL
                     AKTHRDELMEILGEKFPEYEWKKNKGYPTAEHREMIRRYGCTEYHRLSFRLLKEEPF"
     gene            complement(20306..20848)
                     /locus_tag="PLZ54_01210"
     CDS             complement(20306..20848)
                     /locus_tag="PLZ54_01210"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOJ65592.1"
                     /translation="MNTLDLVILVTVFFGIIQGLFRGLVKELTSFAVIIVGIYGAKLL
                     SPLIAKMLESVASFSPQMAHPLAFLFSFILLAIAMFLLAQLFEKTLSIIQLGGLNKFL
                     GAIVGGLKFLLVLSVLLNVFDAMDERFHLINKATKEESLTYSLVLKAGPELWKEVKDK
                     QSINEQNTHQQNKIVPRQNK"
     gene            21105..22202
                     /locus_tag="PLZ54_01215"
     CDS             21105..22202
                     /locus_tag="PLZ54_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65593.1"
                     /translation="MKTKILGLAALLLFATQTPAQSTFTVEARNYDISDNLDLRAVAS
                     IFAESRDLSDFEMRLNDARYQISNLDLNNDGWIDYLRVVETSGGNLHLVVIQAVLDRY
                     TYQDVASIVVEKRNSPQVYVQIIGDPYLFGVNYVIEPIFVRQPVIFSFFWSKNYRPWH
                     SPYYWGYYPRHYRPLRPVEVNIYLGHVHHIIPRDYKFHYHNSVNIYYRDAYVRLHQTV
                     RRNDFERTHPERGFNSRNKNVANKAELNNSSRRSSTEQERRIVNQDNGSRRISTSSSD
                     SRYNSENNSSRRSQNTGRVETQNNARRISNSGRSVETRTSSQNEKNSSRTETLRRSEN
                     STVNTSQRTSENNSRGTRSTNSNSENRSGRR"
     gene            complement(22286..23350)
                     /locus_tag="PLZ54_01220"
     CDS             complement(22286..23350)
                     /locus_tag="PLZ54_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOJ65594.1"
                     /translation="MKFESILLPGIEAKRPIVIAGPCSAETEEQVMETARGLASIGIK
                     IYRAGIWKPRTKPGGFEGVGEKGLPWLKRLKSELGLYTTVEVANAKHVRAALAHDIDI
                     LWIGARTTANPFAVQEIADALEGVDIPVLIKNPVNPDLELWIGAIERIYNAGIHKIAA
                     VHRGFSSYDKKIYRNLPQWHIPIELRRQMPDLPIFCDPSHIGGKRELIAPLSQQAMDL
                     NFDGLMIESHCNPEKAWSDAAQQITPQVLEFILESLVIRDTMQTTENLSALRRQIDEL
                     DNNLLELLAHRMRVAEEIGRYKKEHNMPILQAQRYDEILHNRVEQAIEMGMDGEFMKT
                     VLIAIHEESIRHQMEIMEKS"
     gene            complement(23402..24574)
                     /locus_tag="PLZ54_01225"
     CDS             complement(23402..24574)
                     /locus_tag="PLZ54_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005868247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOJ65595.1"
                     /translation="MLKQLKMEIEPARRVNNVSEYYFSTKLKEIDEMKAAGKDVINLG
                     IGSPDLPPSEATIEALCKEAHKPNVHGYQSYIGIPELRNGFAEWYKKWFGVELNPNTE
                     IQPLIGSKEGILHISLAFLNPGDGVLVPNPGYPTYTSVSNLCEAKVIPYDLDENHEWQ
                     PDFDALEKMNLEGVKLMWTNYPNMPTGANASVELFEKLVAFGKKHNIIICNDNPYSFI
                     LNDRKLSILSVDGAKDICIEMNSMSKSHNMAGWRIAMLASNRQFVEWVLRVKSNVDSG
                     MFRPMMIAAAEALKNPKEWHDQINVVYARRRNIAFKILDLLGCTYDTRQVGLFVWGKI
                     PDRYKDAGELADEILYGKNVFITPGFIFGDKGNRYVRISLCCPEPTLEEVVRRIEH"
     gene            complement(24602..25435)
                     /locus_tag="PLZ54_01230"
     CDS             complement(24602..25435)
                     /locus_tag="PLZ54_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005933644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOJ65596.1"
                     /translation="MKRVAIQGISGAFHEIAAREYFKGEEIEIIPCETFKDVFKAIEA
                     DKSLLGIIAIENTIAGSLLQNHNLLRESGCLVVGEQKLHIEHHLVALPHQKLEEIEEV
                     HSHPIALMQCEDFLESHKHLKAVESEDTALSAKEIADGQIYKRAAICSLFAAEKYGLE
                     ILERNIETNKRNFTRFLIVAEPTLALEMTKNDEINKASLVFTLPHVEGSLSQVLSIFA
                     FYHMNLTKIQSLPIIGKEWEYQFYINLTFNDYSRYKQAIDAILPLIDEFQIMGEYHAE
                     N"
     gene            complement(25704..28331)
                     /gene="alaS"
                     /locus_tag="PLZ54_01235"
     CDS             complement(25704..28331)
                     /gene="alaS"
                     /locus_tag="PLZ54_01235"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOJ65597.1"
                     /translation="MTSQEIRQSFLDFFAEKGHKIVPSAPMVIKDDPTLMFTNAGMNQ
                     FKNIILGNDPVKYPRVANSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     KEAIEWAWEYLTEVLKIDKSRLYVTVFEGSPSEGLERDEEAAAIWSQFLPADRIINGS
                     RKDNFWEMGETGPCGPCSEIHIDIRSEEERAKLDGLSLINQGHPLVIELWNNVFMQYN
                     RKADGTLEELPAKVIDTGMGFERLCMVIQGKTSNYDTDIFQPIIREIGKISGTEYGKD
                     EKIDIAMRVVSDHIRAIAFSIADGQLPSNMKAGYVIRRILRRAVRYGYTFLHQHAPFM
                     YRLIDVLCETMGKAYPELISQKTLIEKVIKEEEESFLRTLETGIRLLERKMEEAQLEN
                     KKEISGADAFVLYDTFGFPIDLTELILRENNFTVNMNEFHAEMQKQKERARNAANIET
                     GDWIVLREGDSHFVGYDCTSCETNILRYRKIKQKNQEFYQLVLSVTPFYAEMGGQVGD
                     SGWLVGENEKIEILDTKKENNLAVHIAKQLPSEITGTFTAVVHLENRQATSCNHSATH
                     LLHEALRTVLGTHVEQKGSYVGPDSLRFDFSHFQKMTKEEIRAVEALANRKIRENLPL
                     NEMREVPVSEAKEMGAMALFGEKYGETVRVIQFGSSKELCGGTHVKATGEIGMVRIIS
                     ESSIAAGIRRIEAITGKAVEKLMDEQQDILSETREILNNPGNLVNAVRKLMEENTELK
                     KQVNTFQQEKLLQLRDRLMAEAKITNGIHVVRYEGNLTADQAKTLAFQIKNLQNENLF
                     FIAGNVMDGKPSLTLLLSDDLVSRGLNAAQIIREIAKEIQGGGGGQPFFATAGGKNAA
                     GLSAAMSKALKFIDEFPSK"
     gene            28533..29501
                     /locus_tag="PLZ54_01240"
     CDS             28533..29501
                     /locus_tag="PLZ54_01240"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ65598.1"
                     /translation="MAKKKYHFNPDTLSYEPIENSLWFKIRRFLFHAFTGIATGTVFF
                     FILASTVQSPEERELNAEKNKMEAQYKVLQRQFNEVQDVLTDLEERDNNLYRVVLQAE
                     PIPFTVRKAIASNTDYYNQLYDMTNSEIVVSTTKMLNTLKKQLYIQSRSYDELVTLTK
                     NREKMLQCLPAIQPVLNKDLKRMASGYGWRIDPVYHTRRFHEGMDFVAPTGTDVYATG
                     DGTIVNAGWEQGYGNCIVINHGFGYETLYAHLSTIKVRPGQSVKRGDIIGLVGSTGKS
                     TAPHLHYEVHYKGQVMNPQNYYFLDLSPEEYDRMVQMSNNFGEVLD"
     gene            29532..30413
                     /gene="murI"
                     /locus_tag="PLZ54_01245"
     CDS             29532..30413
                     /gene="murI"
                     /locus_tag="PLZ54_01245"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOJ65599.1"
                     /translation="MNELQGNKNYSLTKLELSSLPGPIGVFDSGYGGLTILEKIRQRL
                     PHYDYLYLGDNARTPYGTRSFEIVYKYTLECVVRLFELGAHLVILACNTASAKALRTI
                     QQNDLPKIDPRRRVLGIIRPTVEIIDKLTHNGHVGLLATPGTVQSNSYGLEIQKLYGN
                     AITLSSEACPMWVPLIENFEHNSPGADYFFQKNINHLLQTDPQIDTVILGCTHYTLLQ
                     DKIKNLLPPNINIVSQGEIVASSLDDYLNRHPEIDALCTKNASCRFLTTESVEKFSAS
                     ATIFLNEPVLAEHIDID"
     gene            30678..32837
                     /locus_tag="PLZ54_01250"
     CDS             30678..32837
                     /locus_tag="PLZ54_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ65600.1"
                     /translation="MKVYQPNEIKNISLLGSSGSGKTTLVEAMLFEGGIIKRRGTIAN
                     QNTVCDYFPVEKEYGYSVFPTLAQIEWRQHKLNIIDCPGSDDFIGAVVTSLNVTDMGL
                     IVVGAQYGVEVGTNNHFSYAEKYNKPVMLVVNQLDHDKADYEHTLEQLKENFGNKVIQ
                     LQYPLQTGSGFNSLVDVLQMKMLRFKTEGGAPEITEIPDSEKAKAQELHNSLIESAAE
                     FDESLMEKFFEEGILSDEEIYKGLYKGVLERGIFPVICVSAANDAGVSTLMDYLVRIA
                     PSVSDSKRPLTKDGKSVDPNPDGPTSIYVFKTNVEPHIGEISLFKVMSGKLKEGDDLY
                     NVNRQVKERISQIFCVAGQIRNKVEEISAGDFGATVKLKDTRTGNTLNGKGCDYDFVG
                     VEYPEPKYRRAIKAKSEADEEKLNEILTRMHEEDPTWIVEQSKELKQTILHGQGEFHL
                     RTLKWRVENNDKIEIEFKEPKIPYRETITKKARADYRHKKQSGGAGQFGEVHLIVEPY
                     FEDVPIQDVYRFNGQEYKISVRDVQEIDLEWGGKLVFVNSIVGGAIDARFLPAILKGV
                     MDRMEQGPLTGSYARDVRVIVYDGKMHPVDSNEISFRLAGRNAFAQAFKDAGPKLLEP
                     VYDVVVMTPSDRLGDVMSDLQSRRAVIMGMNSEKGFEKLQAKIPLKELSNYSTTLSSL
                     TGGRASFVMKFASYELVPIEIQEKLLKEYEASKKEED"
     gene            complement(32921..33778)
                     /locus_tag="PLZ54_01255"
     CDS             complement(32921..33778)
                     /locus_tag="PLZ54_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013264050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HOJ65601.1"
                     /translation="MSNNLLVGKRGIIFGALNDMSIAWKVAERVVEEGATVTLSNTPI
                     AIRMGTINELAEKINAKIIPADATNLDDLEQVFSQSMEILGGKIDFILHSIGMSPNVR
                     KKRPYDDLDYDLMHTTLDISAISFHKMLQTAKKMNAINEWGSVVALSYIASHRTMYGY
                     NDMADAKAMLESIARNFGYIYGREHNVRINTISQSPTPTTAGSGVKGFDSLMDFSERM
                     SPLGNASADDCANYCVVMFSDLTRKVTMQNLFHDGGFSSMGMSYRALQQYNKSFEGSQ
                     THDGKIIYG"
     gene            complement(33996..35381)
                     /locus_tag="PLZ54_01260"
     CDS             complement(33996..35381)
                     /locus_tag="PLZ54_01260"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ65602.1"
                     /translation="MKKFIVGFFILSIFISSYGQPNDYRRWSITLDAGINNFDGDNNQ
                     NYNQIIPNSAAKASFGFSTELTLSPFWGIGTEFFYFPVSAEDAAKKFNADVFHFTPYL
                     SLNLLNLCNENNHSKWNIYATVGAGVAYYNSTLYKNGIEFDRIRDGWAAVFPVGGLIE
                     YNISSLFAIGAKVQYRLQNKDNFEGTNLKEEAGGYNFKGVTNDNIAMAALSLRWKIGA
                     KSKNHIRNNETRITLGDVFTLANRANIKTDSLAQELDKLKLSLQPGPDDDGDGVPNSR
                     DLEPNTPKGLYIDYNGRGIPEIKVIPETKEIHPEYLQYISASTKDEDKDGVPDNRDKE
                     PNSPKNLCVDFWGRSIAQDKPCAFASVYFDFDKTNLDEEALIEIEKLAEKLNSDPTLL
                     VEIRGYADNVGSNKYNKRLSQERSNRVKAELVFTHNINPQRIISNGKGKIPQPPRAYR
                     MNRRCDFILSK"
     gene            35877..36317
                     /locus_tag="PLZ54_01265"
     CDS             35877..36317
                     /locus_tag="PLZ54_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ65603.1"
                     /translation="MATNRLTEQLPSNFDRYFDNEWEDYFWTDRELAANHTTLPAVNI
                     KENGEAFEIDVAAPGYEKSDIKIEMNEDLLTVSSNKKLDEEKTEGEYTKREFSYQSFL
                     RTFTLPKTVDAEKISAVYENGILKITIPKKEEAKVKPARTIDIQ"
     gene            36382..36678
                     /gene="trxA"
                     /locus_tag="PLZ54_01270"
     CDS             36382..36678
                     /gene="trxA"
                     /locus_tag="PLZ54_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOJ65604.1"
                     /translation="METFQDIINGDKPVLVDFFATWCGPCKAMAPVIESLGKEVQGQA
                     RVLKVDVDKNQALAAKYGIQAVPTFMLFKKGNVIWKHSGATDKNTLMQQIKSAV"
     gene            complement(36755..37195)
                     /locus_tag="PLZ54_01275"
     CDS             complement(36755..37195)
                     /locus_tag="PLZ54_01275"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOJ65605.1"
                     /translation="MEENQDIKYMKQALAEARRAAEKDEVPIGAVIVCQGSIIARAHN
                     LTETLNDVTAHAEMQAITSAANYLGGKYLSDCTLYVTIEPCVMCAGALGWAQIKKIVY
                     GASDEKRGYSKFAPDALHPKTEVVSGVLAEECATLMQDFFKRKR"
     gene            37382..38512
                     /locus_tag="PLZ54_01280"
     CDS             37382..38512
                     /locus_tag="PLZ54_01280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ65606.1"
                     /translation="MKIIIVGTAYPYRGGLASFNERLAREYAREGHEVELITFTLQYP
                     NFLFPGKSQYSTEKPPEDLKIIRRINSINPFNWIKIGKEIKKKAPDLVIFCYWTSFIA
                     PCFATIARYARSPQTKMLGLVHNMIPHEATPLDKWLPRYFVKNMDGFLAMSNSVKEDI
                     QHFDKKNKPKIVVPHPVYDHYGAIVSKSEAALKLGLPPKKRYVLFFGFIRHYKGLDLL
                     LEAFSDKRLKDMEVQLVVAGEFYENPEPYLKQIAQLGLANRVELRTNFIPENEVKYYF
                     SLADLVAQPYRSATQSGVSQIAYHFEKPMLVTNVGGLAEIIPNGKVGYVVNGQPKEIA
                     DAIVDFFQNNREEIFLPNIKTEKMKYGWDKMTAAINKLFFKL"
     gene            38595..41207
                     /gene="mutS"
                     /locus_tag="PLZ54_01285"
     CDS             38595..41207
                     /gene="mutS"
                     /locus_tag="PLZ54_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005849895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOJ65607.1"
                     /translation="MSEPIVETPLMKQYFEMKSKHPDAILLFRCGDFYETFSTDAIEA
                     SEILGITLTRRANGSAKSVELAGFPHHALDTYLPKLVRAGKRVAICDQLEDPKLAKKI
                     VKRGITELVTPGVTYNDTILNHKENNFLASVYLNKNQAGVAFLDVSTGEFLLAEGTNE
                     YVDKLLNSFSPKEIIFDRTKKKEFENLFGTKFYSYPLEDWVFTLDAAKDRLLKQFETK
                     SLKGFGVEGLANGVVAAGAILYYLDLTHHQQTGHITQLSRIEEDRFVWLDRFTVRNLE
                     LFEAINEGGKTLLEVLDKTISPMGSRLLKRWMAFPLKNVKSINERLDVVEYFFKHPEL
                     KEALAQNIALIGDLERLVSKIAVGRINPREVVQLKTALKAIEPIKQLCTHTENITLRT
                     IADQLNACSIISEKIEREIQNDPPILINRGNIIQKGVNADLDELRELAFSGKDFLQKI
                     QERESQATGIPSLKVGFNNVFGYYLEVRNTYKEKVPETWIRKQTLVNAERYITEELKK
                     YEEKILGAEEKIIDIETRLFNELVLSLSEYIPALQLDSNLIAQLDGLLSFAKVSEENR
                     YVRPEINDTEVLEIHQGRHPVIERQLPLGESYVANDVYLDSSKQQIMIITGPNMSGKS
                     ALLRQTALITLMAQMGCFVPVESAKIGVVDKIFTRVGASDNISLGESTFMVEMSEAAS
                     ILNNLSSRSLVLFDELGRGTSTYDGISIAWAIVEYIHEHPAHKAKTLFATHYHELNEM
                     EKSFSRVKNYNVSVKEVDNKVIFLRKLVRGGSEHSFGIHVAKMAGMPQSIVKRAEQIL
                     KQLETDNRQSDISKPVDKIAQHREGYQLTFFQLDDPVLEQIRDEIKNLDINNLTPIEA
                     LNKLNEIKRIIMGK"
BASE COUNT        12867 a         8165 c         7883 g        12517 t
ORIGIN      
        1 ttcgaaccca ggaccccatc cttaaaaggg atgtgctcta cctgctgagc tactagatca
       61 ataataaaaa tgtctgtttt ttttttaaaa cgcttgcaaa ggtacaattt cttttcgaat
      121 tttcaaaaaa tcaatgcaaa actttctgct aaattcacac ttctaagttg aacttagcag
      181 tgaagtagaa aacgggaaaa aacttattgc aatgacccgc ctatcaggtc aaggatttca
      241 tttgttatgc tttcctgtct cattttgttg tattcgagcg aaagttgttg taaaaggttt
      301 tcggcatttt cggtagctgc ttgcatggac agggtacggg ctgcacattc cgaagcataa
      361 gcttcgagag ctgccgtgta aatatgataa tgtaaagcca atggaattaa ttgttgttca
      421 atttcttcga tagaaggttc aatgagataa tcgttacgaa attcgccttt ttttcgatag
      481 tttatttcga ttggcaaaaa ggtttcagct ttcatgattt gtgtggtttt gttcacataa
      541 tgaaaatata taacctttat tcgatcgatt ttgtgttgga gaaaaagctg gataagagta
      601 tcggctatct gagtggtgtt ttcgatattg ggatgagcag ccacattttg aaattcccca
      661 gcaatagcca attttttttc tttgcaaaaa tcctccactt ttttgccgat cggaaaaatt
      721 aggatatctt cctttcttac gtcacgatgg gtggtaatag attgttccag tcttttgatt
      781 atgtttgaat tgtagttacc gcagagagaa gaattggaag aaaataccac aatgccaaca
      841 tgattcacat gcctgatttc tctgaaaggc gatctgtcaa caatttggct acttaaataa
      901 tttctcaata tatccgttaa ggtttcccga taagtcaaga aattgtaaac ggctttttct
      961 gcctttaaaa gttttactga agagaccatc ttcatggctg aagtaatttt ttgagtagat
     1021 tttaccgatt gtattcttga tttaagttcc tttaatgaag acatgatgtg tgaaattgat
     1081 tttttaaaat aaagcttgtt gggagatggc tttaataatt catttgtttt tttaatattt
     1141 ctgctgcctc ttcaatggaa gaagtaactt tttcgtcaaa atttcctttt tttatttcat
     1201 tcagaatttt ttccttgtaa gaataatcca aaagattaat gaaattttct tcaaattctt
     1261 taaccttatg gagtggtata tttgccagca atccatgtgt tccacaatag agtagcgcta
     1321 cctgtttttc cacagataac ggccgatgta gggactgaat aagcaattgg gtattttttt
     1381 gccccttatc tatggtcata gccgtcaccg catccatttc acttccaaat ttagtaaaag
     1441 cttccagctc gcgaaattgt gcctgatcgg tttttaatgt gccggctact ttcttcatgg
     1501 gtttaatttg tgcattccct cctacacgag aaacggaaat tcctacatca atagacggtc
     1561 tattcccttc gttgaacaaa tcggtatcga gaaaaatctg gccatccgta atagaaataa
     1621 cgtttgtggg aatatacgcc gaaatatcgc cggcttgcgt ttctataatg ggcaatgccg
     1681 tgagcgaacc acccggtttc actttccctt taaggcattc aggcaagtca ttcatttgtt
     1741 tggcaacttc ctgctgatta atgatttttg cagccctttc cagtaatcgg gaatgcagat
     1801 aaaagatatc gccaggataa gcttctctac cgggaggacg tcgaagaatc aacgagagtt
     1861 ctcgataagc gatagcttgt ttggacaagt catcgtaaac aaccagtgca tgtcttcctg
     1921 tatcgcgaaa atattcgccg atggcagctc cggcataagg tgacagatac cgcaaagcag
     1981 cagaatcggc tgctgtagcc accaccagaa tggtgtaagg tattgctcca aaatctttta
     2041 gggtgttgaa gagggttgct acagtagaac ctttctgccc tgtggctacg taaatgcagt
     2101 aaacgggatc tcctgcttta aaattgtcca attgattgat gatggtatcg accgcaatgc
     2161 ttgttttccc tgtttgacgg tcgccaataa ttaattcacg ttgtccacgt ccaataggaa
     2221 tcattgaatc aatggccttc aaacctgttt gtaatggttc ttccacaggc tgtcgaaaaa
     2281 ttactcccgg tgcttttcgt tccaaaggca tttcgaagaa ttctccatct attttacctt
     2341 tgccatcaat aggttcaccc aaaggattga taacacgccc caacattttt tcgctaactt
     2401 tgatggaggc aactctgccg gttcttttta cagtgaaacc ttcttttatc aagtgggtgg
     2461 gaccaagcaa cacagctcca acattatctt cttccaaatt catggcaatg gcttttactc
     2521 cattttcaaa ttcaagcaat tcattggctt ctgcatttgt caatccatat attctgacaa
     2581 ctccatcccc tacttcaagt acttttccgg tttcttcata ataactctcg atgtcgattt
     2641 gttggagttg tttttttatc agctccgata tttcacttac cttaatagtc ttattatcag
     2701 acatattctt ataattatat ttttaggttt acgaaaagaa ttttttcagg ttaaaagaat
     2761 attttcgttc aattcttttt gaattttttt tagttgcgtt gccaagcttc cgtcccatct
     2821 tcggtttcca atttcgatga taaaacctcc aataatttca ggagcaatta ttttttccac
     2881 ctttaattct ttcttaatat aagttttgat ttgagaaatc aatttttttt ctgtttcggc
     2941 atccatagga gaagctgtag tcagttttac caatgccaag tttttgtttt cgtaataata
     3001 atcggaatat tttaaaccta tatttcgcaa aagatattca cgacggtttt gcaacaatac
     3061 atccataaaa cgctctaaaa tgaaatcaga tttgttacca caggcaaatt ggataagttg
     3121 cttttttgtt tcccttgtca acaaaggatt ttttaatgcc tgcgctagtt ctgcattggc
     3181 agaaaaagat ttttcaagtg tctgaaagtt ggcaaaaaca atttcctgcg cattcatttc
     3241 agaagcgaat tcatacaggg cttttgcata acgtgaagct atcaaaccgg tttgcatgca
     3301 tataattgtt acaatagata ataaaatttt taaagtggag ttttattgct gttcggaata
     3361 aataaatttt tcaatttcca aatttaccga tatccatctt ttccaacatc cgattgataa
     3421 tttccatttg ggcgggctta tcatttaatt tcgattgtaa cagcttttcc gataccgcaa
     3481 ttgaaatctc agcaacctgt tgttttatct cctgtaaagc catcattttt tcatacctga
     3541 tttgtttttt tgcttcttcc atgatttttt cagcttctat aaaggctttt tcctttgctt
     3601 cttttattaa agcattttta atttcagtag tttcatcaag tatttttttc cggtttaaaa
     3661 aagcttcatc ctgtattttt tttgcttctg ttttaatttc ttcgagttct ttgcgtgcat
     3721 tttcggcttc caacaaagat tcttcgatgt aagaccttcg tttttctatc atttgaagaa
     3781 tggcagggaa accaaattta acaagaataa aaacaactat tccgaatgaa agcatcatcc
     3841 agaaaaataa accaaaatcg ggcattagaa gagacatagc atttttttaa tgattaagca
     3901 cacaattaat agaatcaaat acttttgatt tttccaatat tatcaatgaa taaccctcct
     3961 gagacaagcc ttgaaaaatt tttttgatta aagaataaat ccacaaacta caatggcaaa
     4021 caaagcaact ccttcaataa gagccgcagc gataatcatg ttcattcgaa tatccgaact
     4081 catttcaggc tgacgcgcaa tactttccat agccgatccg ccgattttcc cgataccaat
     4141 acccgctgct atagctgcaa gacctgcccc caatgcggct ccaaattttg ccaatgcatc
     4201 acttgcggca acttgtaata aaatagatag taacatcata acttcttgtt tttaaatgaa
     4261 atttttataa tgatattgaa ttaattaaag tctaatatta gtttttcgca ttttcaaatt
     4321 gttcaaactt gtttaactac ttcctttgct gcctgtttat gggaaggctc tactcttgcc
     4381 aatccgataa acacagccga aagcatggta aaaatataag cctgaatgta tgctatcagc
     4441 aattctatca ggtcaatgaa aaccgtaaaa atcactgaaa gagccgacat ggttacattt
     4501 atggttgttc ccaattttac cgtaacgaac accagacaaa ccaatccgcc aactatagaa
     4561 tgccctccca caatattggc aaagagacga atcattaaag caaagggttt ggtaaaaatt
     4621 ccaataaact ctacagccgg catcaaagga acaggaactt tcagccaagc aggtacttca
     4681 ggccaaaaaa tttctttcca atattccttt gttcctgaaa agtttacaat taacattgta
     4741 aaaagagcca gcgtaaatgt aatggcaatg tttcctgtta cagtggctcc aaaggggaaa
     4801 aacggaatca atcccatcaa gttattgaga aaaataaaga aaaaaaccgt cagcaaatag
     4861 ggcgcatacc gtcggtaatc attcccgata cttggcttga taacaccttc gtaaatgtca
     4921 acaatcagca tttccatcag gataacaaat ccttttttgg ggaaaattcc atcttttttg
     4981 taataatgag ctatactcat gattaaaata atcagcaaaa cggaattaaa aataagcgca
     5041 gtagccgttt ttgttaagga aatatccaaa ggccttattt ctttaccatt ttcatcgagt
     5101 tccacaattt tatccttgta ttttccttct tttgcaatat agaaaccctt gtaaacaggt
     5161 tgttcttcaa atttggaaga cataaaaaca tgccatcccg aattattgct gtgaagaatg
     5221 acaggcaaag gaatcgaaat tgatttgctt ttccaggtag taatatacca ttcataggaa
     5281 tcactcactt ctgtaagaat gatttcccga acatcaattt cttttctttt cccctcataa
     5341 ttattgttct gggcaagtat tttaaaaggg gaaaaagcaa gaaaacaaat aatatagaca
     5401 aaacagcatt tcatttactt tgccgaactt gacgtttttc gttcatataa aaacaagcag
     5461 tctcaaaaat cagataaatt agataataag caccataaag gagaccaaac atccttcgtt
     5521 ctgttctaaa aaccaacaaa taaacaaaag ctacgattcc ccataaaatc atttttgaaa
     5581 gctttaacag taaaaacaga taaatttttt tagtagatga aatataagaa aatttttgta
     5641 gcacaagcat taaagtcaat ccagaaaaat aaaacaataa aggcaacaaa aaataatttt
     5701 cgatagtcgt ggaagaaaat tttttcaaga gcaaccagaa aaatagccct aacaacaatg
     5761 ttagtaaaga aaaaaaaact aatgaaaaaa gaagattatt tttcattttc tggaatttgt
     5821 tcagcacaaa taacgacctt atcgttattc aattcgatta gtccgccatt aataaaaaca
     5881 ttgttaacac cgtttttgaa acgaaaagta acatttcctt tccccaaaat ggcaatcaaa
     5941 ggagcgtgat gagaaagtat ggtaaatacc ccccccttac ccggtactgt tacagatgaa
     6001 atttcaccat cgtaaatcaa tttattgggc aaaacaattt tcagattcat tgatcctcaa
     6061 ttttaaatgt tttattcaaa ttgtgagctt ctcttagcaa tgcatgcccc ttatttaaga
     6121 catccttaat ggtaccaaca ttaagaaaag ccatttcggg aaggtcatcg agcataccgt
     6181 ccaatataag tttaaagccg tgaatggttt cttctatagg taccattact cccggaactc
     6241 ctgtaaaatg ttctgccata aagaaaggct gcgaaagaaa acgttgtact tttcgagctc
     6301 tttgcactgt taaacgatct tcttcagaga gttcttctat acccagaatg gcgataatat
     6361 cttgcaactc cttgtttcgt tgaagaattt gcttcacccg ctgagctgtt tcataatgtt
     6421 cctgcccgac aataagcgga tcgagaattc gtgaagtaga atccagtgga tcgacagcag
     6481 gaaaaattcc caatgaagca atttgccgac tgagaaccgt tgtagcatcc aaatgtgaaa
     6541 aagtagttgc cggagcagga tcggttaaat cgtccgctgg aacataaata gcttgaacag
     6601 aagtaattga cccactttta gtggaagtaa tgcgttcctg aagaagcccc atttcagtag
     6661 caagtgtagg ttgataacca acagcagaag gcaatcgacc caacagagcc gaaacttcag
     6721 agcctgcctg agtgaaacgg aaaatattat caataaacaa taaaatatca tgattctcac
     6781 tttcatcact gtcgcgaaaa gattcagcta ccgtaagccc cgacaaggca acggaatatc
     6841 gtgctccggg aggttcgttc atttgtccaa agaccaaagt cgcctgagat ttttttagtt
     6901 gctcataatc aactttcgat aaatcccatt tcccttcttg cattgctttg ttaaattctt
     6961 caccatagcg aataactcct gcctctatca tttcacgcaa caggtcattc ccctcacggg
     7021 ttcgttcacc aacgccggca aaaaccgaaa atcctttgtg ccatttggct atattgttaa
     7081 taagctccat aatgataacc gttttgccaa caccggcgcc gccaaaaagt ccaatttttc
     7141 cacctttaac gtaaggagca agtaaatcga caactttaat tccggtatat aaaatttcgt
     7201 cgttgtttgc aagttcatca aatttggggg gttctttgtg tatgggagcc gactctaccc
     7261 ttcccagtgg gcttaagcca tctatcgatt cccctatgac attcatcaat cttcccttta
     7321 cttgattccc tactggtatg gaaattggcg aacccaaatc gcttacgggc agacctcgac
     7381 ttaacccatc agtggaatcc agcgcaattg ttcggacaat attctcgcca atatgttgtt
     7441 gaacttctaa aatgagattt ttcccgttgg aaagcgaaat actcaaagcg ttatttatag
     7501 gaggcaattc aaattcattt ttctcttcga gagggaaaaa aacatccact accggaccaa
     7561 ttatctgcca cacctttccc tgtattgata acatgaaaaa attataattg taaatacatt
     7621 ttgtgagaaa ttgcgtggca aaagtaaata aaagattctt cttaaaagaa aacgaatgtt
     7681 taaattagtt caaaaaaaca aatctttctg ttaaacaata ttattcagtt gttcaatcca
     7741 acttcaaaac agctaaaaag gccttttgtg gtacttcaac cgatccgatt tgcttcattc
     7801 gttttttccc ttttttctgt ttttccagca gtttacgctt tcgtgaaata tcgccgccat
     7861 aacatttggc agtaacatct tttctgacgg gtttaatagt ttctcgtgca ataattttgg
     7921 ccccgatagc tgcctggatg gcaatttcaa attgctgacg gggaatcagt tccttcaatt
     7981 tttcgcacat tcgccgcccc aatgaatagg cattatcggc ataaatcaat gaagaaagtg
     8041 cgtcgacagg ctctccgttg agcaaaatat ccagctttac cagtttggaa gctctgtagc
     8101 cattcatgtg atagtcgaaa gaagcataac cttttgaaat gcttttcaac ttgtcgtaaa
     8161 aatcaaaaac tatttcgctt agcggcaggt cgtaaatcaa ttcaatccga ttgcccgaaa
     8221 tgtattcctg cttgatcagt tctccacgct tacccagaca taaagtcata atgggaccga
     8281 tatattccat cggagtgata atggaagctc tgatataagg ctcctcaatg cgatctatca
     8341 aagtcggatc gggcatttcg gaaggattat gcacctcaat aagttctccc tttttggtat
     8401 aaattttata ggaaacattt ggaacggtgg taataacatt aatgttgaat tcacgttcca
     8461 gacgttcctg aataatttcc atgtgcaaca ttcccaaaaa tccgcaacgg aatccgaatc
     8521 ctaaagctgt ggaagattcc ggctcgaaag tgagcgaagc atcgtttaac tgcaattttt
     8581 ctatggaagc ccgtaagtct tcaaaatcct ctgtatcaat cggataaaca ccggcaaata
     8641 ccataggctt aacttcttca aaaccggcaa ttgctttttg acagggacgt ttcacatgtg
     8701 ttatcgtatc acctaccttt acttctttgg atgttttgat tccggaaata atatagccca
     8761 catttccggc gctcaaagtt tgtacaggtt gaggttcgag cttcagcact ccaatttctt
     8821 cggcataata atctttgcca gtagcaacaa attttaccca atctcctttg ttgagtgttc
     8881 catttacaat tttaaagtag gcaataattc ccctgaaagg attaaaaatg gaatcgaaaa
     8941 tcaaacattg caatggcgct tcagggtctc cttgtggtac agggatgcgt tctacaatag
     9001 cattaagaat ttcttcgact ccttgccccg tttttgcact agctcgaata atttcttccc
     9061 tgctacaacc aagcaattct actatttcat cttccacttc ttccggtctt gcgctgtcca
     9121 tatctatttt gttgatcacc ggaataatgg tcaaattatg atcaagagcc atgtagaggt
     9181 tcgaaatagt ttgcgcctga atgccctgcg ttgcatccac tactagcaaa gctccttccg
     9241 aagcagctat ggaacgcgaa acttcatacg aaaaatctac atgtcccgga gtatcaatta
     9301 aattcagaat ataatcaatg ctttgatact gataattcat ttggatagca tggcttttta
     9361 tagtgatgcc acgttcacgt tccagctcca tattgtcgag cacctgttcc tgcaagtctt
     9421 ttcccacaac cgttttggta tattccaata aacggtcggc aagcgtactt ttcccatgat
     9481 caatatgagc aataatacaa aagtttcgga tgttgttcat tcgattttct tcaaatttta
     9541 agaactacaa aaatacatga aaattcaaaa ttaacataca aacggataaa aatagatttg
     9601 tagaattgtt tgatttgtat tagaagaaat agattaatct aatgatataa atcaaattat
     9661 cgaatggcgt acattcttat aataaattga aaatcaatgc tttagttata ttaaaccttt
     9721 ttaattaacg tttcgataaa tcgttgtaaa gcatttgaag ataggcttca ccttttttct
     9781 tgttggcatt cggttgtccc caattccaaa gcactttgtt tgtactgcag tcaaagacgt
     9841 aatgattttc aggggttaaa aaaccgaaac cattagggaa cgtgtaaaaa gcaaaatggg
     9901 gagaagctgg attcaggata tttttgctga aagtaaaatc ttgatgcggt aattcaagtt
     9961 gataaagtag ggttgcagca atatctattt gggacgcata cgtatctatt ttcattggcg
    10021 attttacagc accaccaata atgagcagtg gaatttgata cctttcaacc gaagaaagta
    10081 gaatattttt tggataatgc atggcatggt ccggcacaag aaccaaaaca gtattttccc
    10141 agtatttggt ttttctgaat tcgtcaacaa atgcgcccaa acaactatcg gtatattgaa
    10201 ccgaatttaa atagggatcg ctgtttttat aaaaggggac atcgaaaggc tcgtggctgc
    10261 tgagtgtctg aagaattttc ataaagggtt ccttttgttc ttttttcaaa tcattggtta
    10321 aacgctgaaa aacgatgtga tcgtgcgcac cccatttcgt tgttctgtct ttgagcggaa
    10381 aatctttgtc aacaaccatt ttgtcgatgc cttgagcaat caggtaagct ctcatgttgg
    10441 tgaagtccac atcgcctcca tagtagtatt gcaaatcgta acctgcagtt ttcaaagttt
    10501 tgggaatgga aggcaaagac tggcttttct tgggatattt cataatggaa gttgtgggct
    10561 gagccggata gccgctgaaa atggaaatca gtccgcgatc ggtacgaaaa ctgtttgcgt
    10621 aaaagttggt aaacaggatg ccttcatcgc aaaattcatt catgcgtacc gcaattccgg
    10681 gaaaacctcc cagcggctca aggttgttgg acatgaaact ttccaccact acaaaaataa
    10741 catttggttt cggactggta aataatttag gtagttcttc ctgtaccggc tggtcgatta
    10801 aattattaaa ttctttttga gcctcttctt cagacatgaa gcggtattgt ttgtcaaaat
    10861 tttgtgtttt gaatgccgat tccaaaaaat tgaacatggg attaatggca gcatgattca
    10921 actcattttt cgggctgaaa taaaccttgc tcacattcat ggtggaaacg ttaaatccgc
    10981 ctctgatggg aataaacagc aggcaggtaa acaaaaagag caagcaggct gtagaaagtt
    11041 tgtttttgac ctgtttggat tctttgttga ttttgataag caaaaagtaa aaaattgcgt
    11101 acaaggttaa agcaatcaga atgatagcca atagcccgac aacaatcatc caaatatcga
    11161 tgcttgccaa tgcttctttc ggccatttca tgtaaaacag tacagtggaa tcgagacgaa
    11221 aaccccaaaa tggatacagt gcagcgtcaa ccacaaaaac aacggaaatg ataaacgaaa
    11281 taagaccaaa atatattttt tgaaagtatt cgtgaaccga aggttttgcc cataacgaaa
    11341 caattaagaa aattcccggt aaaatagtga gataagccga catggacgca tccagcttta
    11401 ttccgtgtag aataattagt attaaatcct gaaaacttag attactgtaa atttgatgat
    11461 tataaagaat gaaaaatggc ttttgaagca caaaaacaaa aagtatcaac aaataaattt
    11521 taaaaaattg aattattttt tgcaacatta gaagttattt gaaaaagttg gaaatttgga
    11581 aaaaaatact ttcaagcctg attttaagaa aaatcggaca aagttaaacg attcctttca
    11641 aaagaataga ttttctgaag atttttttgg aattatattt taaactcgat aatatatgaa
    11701 acccacatgt attacataca caaacacgaa tgaatataat attcagacat gtgggttcca
    11761 tcagaccttt aaaaaataaa ttatattctg atgaaaattg tcaaaaaact tttttaaata
    11821 tttttcttcc tttttatctc aatgtcttaa aaaaaccaat tctctttgag taacttttca
    11881 gaggagactc ttcctttaaa tcagttatga gccgagtata aagacttttt tattcgtaat
    11941 aaaaattacc aatttaaaat gcgataaaaa ttatttcaat atggctgtca atatgtcacc
    12001 ggaaaaagca ttggcatttt ttttgcaaaa aatcggcggg aaaagttggg aatcagagat
    12061 tttttcgctt ttccaacgca taaaaggaat ttaaaaactt attaaatcca aaataaacat
    12121 gacaaaaggg actatagggg ttacgagtga aaatattttc cccgtgatta agaaatttat
    12181 gtacagcgat catgaaattt ttctgcgcga attggtttcc aatgctgtag atgctaccca
    12241 aaagcttaaa actttggctt cagtgggcga attcaaagga gaattgggag atttgaaaat
    12301 ccgcatttct gtggacaaaa agaaaaaaac catcaccatc agcgaccgcg gaataggcat
    12361 gaccgccgaa gaaattgacc agtttatcaa tcaaattgct ttttccagtg ccgagcaatt
    12421 tctcgaaaag tacaagaacg atgcacaatc cattattggt catttcggac ttgggttcta
    12481 ctctgcattt atggtttcaa aaaaagtgga aatattcacc ctgtcgtaca aggaaggtgc
    12541 ggttgcccaa cactggtcgt gcaacggaga cccggaatat gtacttgaag ataccattaa
    12601 atcggacaga ggaacggatg tcgttttaca tttggatgac gataatcttg aatttttgga
    12661 agaatcgaaa attcgtgaac tacttttgaa atactgtcgt tttctccctg tcgaaattgc
    12721 ctttggaaaa aagaaggaat ggaaagacgg caaatatgtg gaaactgatg aagatgagat
    12781 tatcaacaat acgcagcctg catggattcg caaaccgagt gaactgacgg aagaagatta
    12841 tctgaatttc taccgtgaat tgtttcccta taacgaagat ccgctgtttt atattcattt
    12901 gaatgtggat tatcctttcc acctgaccgg aatcctgtat ttccctaaaa taaaaaacaa
    12961 tatcgacata cagcgaaaca aaattcaact ttactgcaat caggtatttg taaccgatca
    13021 tgttgaaaat atcgttccag aatatttaac tctgctgcat ggcgttatcg attctccgga
    13081 tattcctttg aatgtatcca gaagtttttt gcaaaacgac tcgaatgttc gcaaaatttc
    13141 caaccatatt accaaaaaag tggccgatcg cttgcaggaa atctttaaga atgacagaaa
    13201 tcagttcgaa cagaaatggg acgacctgaa actgtttatt cagtatggca tgttgaccga
    13261 cgaaaaattt tatgaacgag ccgaaaaatt tgccttgttc aaaaatgttg atggcaaata
    13321 tttcacttac gaagaataca aaaccctgat tggcgaaaat cagaaagaca aaaatggcga
    13381 tttgatttat ctgtatgcaa ccaacgccga agaacaatac agttacatac aacacgccaa
    13441 agacaaaggc tatgatgttc tgcttatgga tggtcaactg gatattcatc tcataggtca
    13501 tttggagcag aaatgggaaa aaacgcgttt tgtacgggtt gacagtgatg tgatagaaaa
    13561 actcattcag aaagaggata ccgccaacgt ttcattaagc gaagcacaac gcgaagcatt
    13621 gactgcaatt ttcaattctc agttgcctcc aatggaaaaa acaaacttta tcattacttt
    13681 tgaacaactg ggacccaatg taaatccggt actgataact caaaacgaat tcatgcgtcg
    13741 aatgaaggaa atgtcggcca tgcaaggcgg aatgatgaat ttctacggag aaatgcccga
    13801 tagctataat ctggtggtaa atactaccca tccgttgatc gaaaaattgc tgaaggatga
    13861 agaaacggct tgtgccgaag aattggcacc gattgaagcc gaacttttgg aattgagcaa
    13921 caaactccaa caactgaaag agaatcataa aaacaaaaaa gaggaagaaa ttccggcaga
    13981 agaaaaagag gagttgacaa cgttggaaaa aaggattgat gaactgaaag aaaaaagaaa
    14041 atcggttctt gcagcctttg caagcaacta taaaacagtg cgtcagttga tcgatctggc
    14101 acttctgggc aacaacatgt tgaaaggaga agctcttgcc aatttcgtga aacgaagcat
    14161 agatttaatg taaaaatttc tttctgcttt acttgttgaa gctcgtcgga ttttcctaaa
    14221 ctgacgggct ttttttgtgc agatattttg aacatgcagt tttttgtatt acttttgcaa
    14281 cttcatttta tgtaactaac tgattactaa aataatgaga aagaaaacga ctttattttt
    14341 cgccttttta ccgctggctg ctatggctca gaattttcaa ctgcattacg attttggaca
    14401 ggggcgacat tacgttacaa cgactttcga aatgttcaaa cccgatgatt ggggaaacac
    14461 tttctttttt gtggattacg atttcaacat ggatcaagat cataatgcaa gcctttctta
    14521 tatggaattg gcacgatgtt tttcattggg taaaacgtct cctttcagtg ttcaggtgga
    14581 gtacaacggc ggtttgtttg ctatggaagg atctgcttat cccattcaaa acgcttttct
    14641 gatcggactc gactatggct ggcacaacaa caatttcgat cgttttctga attttaaagt
    14701 gctttacaaa aatattgttg gcaaacatcc tctctctttc caacttacgg gagtttggga
    14761 tttaagcttt tacaataaaa gaatttccgt ttgtggtttc gccgactttt ggtgggaaga
    14821 taatctcaat ttcacagatg ccgccggtaa aaatctcgat tcaccggtgc ttactcggta
    14881 cgtctttatt tccgaaccac aattctggta caacattacc ccacatcttt ctgccggttc
    14941 ggaaatagaa atagcagcca acttttcctc cgtttatgga ttaaaaattt gtcctacatt
    15001 gggaatgaaa tggaatttct aaaatgaatt tcccctgaaa cattttagaa aaaagcatac
    15061 cgaaaaattc atttcaattc attatctttg ctgattgtaa tctcatttta aatagaaaat
    15121 gaaactgttg aacaaaaatg agcagcaaat ttttatcgca gaaaaacagc attcagtgaa
    15181 aaattttttg gtatttattg taatggccgg atttttggtg tcctgtcatt cggcaaccca
    15241 aaaatctgct aatgaagaaa aagtcataaa tattcctgtg tttgactcag attcggcttt
    15301 ttcgtttgtt cagcgtcagg tcgattttgg accccgtgtt ccaaatacgc ccgcacacga
    15361 tgcttgtgcc aatttttttg ttgcaacact gaaaagtttt ggggcacaag ttacaactca
    15421 acaaatggac ttgcggactt ttgatggaac tttgcttcat gctgtgaaca ttattgcttc
    15481 ctatctaccg gaatcggaaa caaggatttt gctttttgca cactgggact cgcgcccctg
    15541 ggccgacaac gacccaaatc caaaaaatcg ggaaaaaccc gttttgggag caaatgatgg
    15601 ggcaagcgga acgggagttt tactcgaaat agctcgacaa ttggggaaaa acccggcaaa
    15661 tgtgggagtc gatataattt tttttgatgc cgaagactgg ggagcatcca atgatgacca
    15721 aacttccaat acggaaaact cgtggtgctt gggcgctcag tattgggcaa aacatcctca
    15781 caagcaaggt tatcatgcac gatacggcat tttgctggat atggttggag caccacaggc
    15841 aactttttat cgggaacaat attcggaata ttatgctgct catgttgtgg acaaagtttg
    15901 gtctcaagcg caaaatctcg gttttggtca gtattttatc aaccagaaag gtggaggaat
    15961 catggatgat catgtttttg tgaatcaaat tgcaggaatc ccgagcatca atattatcca
    16021 ttttgatccc aatacttcct cggggttcgg aaattactgg catactacgc atgacgacat
    16081 gagtaatatt gataaaaata ccctttttgc cgtaggaact accctgatga atgtaattta
    16141 caacgaaaaa taatatatgg aatttcgttt taaaagaatt gcgataaaaa ttggaagtaa
    16201 tgtccttacc aaatctgatg gtacgctcga taccgaaagt atgtcgaatt tgacaaatca
    16261 gattgccaca ctgtatcatc aaggaataga aatagttgta atttcttccg gagcagttgc
    16321 ttcgggtcga agcatcctgg gactgaacaa aaaaatggat atcgttgacc agcgtcaatt
    16381 atattccact gtaggccagg caaaactcat cagcttgtat tgggatttgt ttgccaagca
    16441 ccatattacc attggacaag tgcttaccac caaacagaat ttcgacagcc gaagaaatta
    16501 tctgaatcaa cgcaactgca tgcgcgtaat gctcgagaac aaggttttac ctattgtaaa
    16561 cgaaaacgat gcagtttctg tttctgaatt gatgtttacc gataacgacg aactttcggg
    16621 tttaatagcc tccatgatgg ggatggaagc acttttcatt ctcagcaacg tggatggcat
    16681 ttatcgtggt tctccttccg acccaaaagc agaagttatt cgggaaataa atagaggaca
    16741 ggatatttcc gattttattc agaccaccaa atcaagtttc ggacgtggag gaatgcagac
    16801 caaagtttcc attgcccgaa aaatagccga tcagggcatt gaagtattta ttgccaacgg
    16861 cagaaaggaa aacattttgg ttcgcttgat agatgccgct gacgatgtgg tttgtacaca
    16921 ttttatcgga tacaggggcg aagtatcgag cgtaaaaaaa tggattgctc atagtgaaga
    16981 ttttgccaaa ggcgaaataa aaataaatac caatgccgta aaagcgctga tgggcgataa
    17041 agccgtgagt ttgctgcctg tcggtgtaac ggaaattatt ggagatttcg aaaaagacga
    17101 tattgtgaaa attgttgatg aaaacgggca gtcgattgga gttggaaaag ttcgctacga
    17161 cagcaaaaag gcaagagaat taataggaaa aaagtatcag aaacctttgg tacactatga
    17221 ttatctttat ctggaataaa ttgatgaaaa aagtttctaa aaatgttgat tatttgacat
    17281 ccagtttatt aaattaattt tttacccgta tctcattcat tttttgcatg agttacaatt
    17341 ttgcgcacag attttgaatg gtgaaggatt aaattggttt tgtgagagaa aaaagcaagg
    17401 cagcgtctat gcgaaacata aaaataagac ttaaaattga aacgattgca gtggctttac
    17461 tgctaacggg atgtaacaca acaaaattcg ttccggaagg agaatatttg ttggacaaag
    17521 tgaacataaa catagacacg cggaatgtca aaaaaatcga attaaaagaa tatttgcgtc
    17581 aaacacccaa ttcttcggtt tttggtcttt tccgtaccca acttggcatt tacagtcttg
    17641 caggaaaaga cacttcaaag tggatcaaca aaacactcat gcgcattgga gaagaaccgg
    17701 taatttataa tcctgccctg acggaaattt cctcacagca acttcagctt ttcatgcaca
    17761 ataaaggttt tttgagagcg aaagtggaaa acaaagttac acttaaggat aaaaaagccg
    17821 aagttgaata ccaaattctt gctcatcaac cctaccgtat ccggaattat tcggtacagt
    17881 tgaaccatcc cgaactgcaa gctattgcag ccgacacttc ccgctcgctc attcggccgg
    17941 gaatgctttt tgacgtggat atgctcgatg ccgaacggga aagaatcact tctcgttttc
    18001 gccaaacagg ctattacaat ttcagtaagg attttatagt ttattcggcc gacagcatag
    18061 aaaaaacgca tcaggtggat ttgtccatgc aattgagaga ttatctgaac gacaaaaacg
    18121 actcactttc ccaaatcata ttcagaaaat tttatgtgag aaacgtgtat ttcgaaatta
    18181 accccactga ccttccatca ctgaatcaac ctatggaaaa ggatacggtg tggttccgaa
    18241 attttgccat gataactcca cacaagcaaa ttattaaaat tgacgcattg gttcagaaca
    18301 catatattga tccacaagct ttatttaacg atgccaacat agaaaaaact tattcaggac
    18361 ttaattcgct gggtgccata aagtttgtca atatcggttt tgaacaggtc gatacgggtc
    18421 tactcgattg ccacatcagc attattccgg ggaaagccat ttctctttct gctgaagtcg
    18481 aagccactta tacggatgga tactggggtg gagcaaccaa tttgaacctt gtcaatcgaa
    18541 atatttttaa aggtgccgaa actttatccc tgcaggttca tggagcttat gaacttcagg
    18601 aaaaggtgtg ggcacaggaa tggggagggc agattgggct gcaatttcct cgtgtggttt
    18661 taccatttgc ttcttatgat ttcaagagaa accttcgggc taacacggaa ttttccggtt
    18721 catactatta tcagtttcga ccgggcgaat ttcgaacaag caacttgggt gccggcatga
    18781 gatattttct caacagaaga caatataccc acatttttga tttgtttgaa gtcaactatg
    18841 tatattttcc cgaaattttc cctgctttcc gcgacagttt tctcacaacc ggcattttta
    18901 attcttacaa ttacgaaaat cactttatca tgcgaatggg ttattcaggc tcttccacaa
    18961 actttaacgc caatcaaccg ttacggaact accgtaccat gcgttacagc atagaaacgg
    19021 ccggaaatat tctatacgga ctggatcatt tgctgggcag ccgtccggcg gaagatggaa
    19081 attaccgcct gctcaacatt gcgtatgctc agtatttcaa aacagaatat aatctaactt
    19141 atcatcaaat tttcgatgaa aacaatcgtt ttgtttacca tgccggatta ggaatagcta
    19201 ttccttacgg aaatgccgat gcagttccgt acgaaaaacg atttttcagc ggaggagcca
    19261 atggcgtcag aggttggagt gaaagcacat tggggccggg tgtttataaa agaatcaata
    19321 cccaacgcag agatttcaat cagaccggcg acatcaaact cgacatgaat atggaatatc
    19381 gcggaaaatt gttttggttg ctcgaaagtg cggtttttat ggatgcagga aatatatgga
    19441 ctatcaagga ttatgatacg caacagggtg gattgttcaa atgggattcc tttatgaatc
    19501 aaattgcagt tgcttacgga gtcggactaa gattcgattt ttcctttatc gttgcccgtc
    19561 tggatatggg agtaaaactt tacgacccgg tactgaaacg cagcgaccga tggagggttt
    19621 cgccgcaatg gaacgacgtg gctttccaca ttgccattgg ttatcctttc tgacgagcat
    19681 ttttccggtt gcagttctgt ttggttaaaa tggttcctcc ttcagcagac gaaaacttaa
    19741 tcggtgatac tccgtgcagc cataccgacg aatcatttca cgatgctctg ctgtaggata
    19801 tcccttgttt ttcttccatt catattcagg aaatttctcc ccgagtattt ccatcagttc
    19861 atctctgtgc gttttggcca ataccgatgc tgcggcaatg gaaagatact tgctgtctcc
    19921 ttttacaatg gtttgaaacg gaatatcggc ataaggctta aaccggtttc cgtcaacagc
    19981 aataaattga ggtttgatct gcaaaccgtc gatggccttg tgcatggcta aaatagaggc
    20041 attcaatatg ttaattctgt ctatttctcg attatcgacc gaggcaaccg accatgcaag
    20101 tgcttccttt tctattattt ttctgaattt ttctctaagc aacgggctca attgcttcga
    20161 atcgttcaat tgaggacaaa tgaaatccgg aggcaaaatg actgctgcag ccacaaccgg
    20221 acctgccaaa catcctctgc cggcttcgtc gcaaccacat tcgtgctgaa aagaagaaaa
    20281 actccgaatg aggctgggtt gttttctatt tgttttgtcg gggtacaatt ttattttgct
    20341 gatgggtatt ttgttcattt atcgattgct tgtccttcac ttctttccaa agttccggcc
    20401 cagctttcaa aacaagggaa taagtcaacg attcttcttt tgtggcttta tttataaggt
    20461 gaaaacgttc gtccattgca tcaaaaacat tcagtaaaac gcttagaacg agtaaaaatt
    20521 tcaatccgcc tactatagca ccaagaaatt tgttcaatcc acccaactga atgattgaca
    20581 aggttttttc aaacagctga gccaaaagaa acatagcaat ggccaaaaga atgaaactga
    20641 ataaaaatgc caacggatga gccatttgcg gagaaaaaga agcaacactt tcaagcattt
    20701 ttgcaataag cggactcagc agcttggctc cgtatattcc cacaataatg acggcaaaag
    20761 aagtcaattc ttttactaat cctctaaaca agccctgaat gattccgaaa aaaacagtaa
    20821 ccaaaataac caaatccagt gtgttcatct tttgccttta catattttta ctgcaaattt
    20881 atgaaaatag ctttcttgtt ttcatgttga agaaaaaatt ttaaagaaag tttcaaaaac
    20941 aataaaaaat tttcacctaa tttattgcta ttctgtcaat tgtattaata ggatatggat
    21001 aattgaaaat ttggcatgtg ttttgacttt aatttatcac catgcatgag tgccttttaa
    21061 aaagtaaaga aatttgaatt atcaaactta aaaaagctgc gattatgaaa acgaaaatat
    21121 tgggtttggc tgctttatta ctgtttgcca cccagacgcc ggcacaaagc acttttactg
    21181 ttgaagctcg aaattacgat ataagcgata atctcgattt acgcgcagtt gcttccattt
    21241 ttgcagaatc aagagacttg tcggattttg aaatgagact gaatgacgct cggtatcaga
    21301 tttcaaatct tgacttgaac aacgatggat ggattgacta cctgcgcgtg gtggaaacat
    21361 cgggcgggaa tttgcatctt gtggttattc aggcagtgtt ggacagatat acttatcagg
    21421 atgtggcttc catagtggtt gaaaaacgaa attcacctca agtatatgtg caaataatag
    21481 gtgatcctta cctttttggt gtaaattatg ttattgagcc gatatttgtt cgccaacctg
    21541 taattttcag tttcttttgg agtaaaaatt atcggccgtg gcattcaccg tattattggg
    21601 gatattatcc aagacattat cgaccgctca gacctgtaga agttaatatt tatctgggtc
    21661 atgtacacca tatcataccg cgcgactaca aatttcatta tcacaactct gtcaatatct
    21721 attacagaga tgcttatgtg aggcttcacc aaacggtaag aagaaatgat tttgagcgga
    21781 cacatcccga aagaggtttc aacagcagga acaagaatgt tgccaacaaa gccgagttaa
    21841 acaattccag cagaagatcg tcaaccgaac aggaacgcag aattgtcaat caggataatg
    21901 gaagtagaag aatatctacc tcttcgagcg actcacgcta caacagcgag aacaattctt
    21961 caagaagaag tcaaaatacc ggaagggttg aaacacaaaa caatgccaga agaatttcaa
    22021 attcaggtcg ttcggtagaa acgagaacgt cttcacaaaa cgaaaaaaat tcatctcgaa
    22081 ccgaaaccct acgccgatcg gaaaattcta ccgtaaatac atcgcaaaga acttctgaaa
    22141 acaattcaag gggcacacgt tccaccaact caaactccga aaatcgttcg ggcagaagat
    22201 aaaaacaact tacgattaaa cttttcagac cggcaactca gcaacaaaaa atgcgaattt
    22261 gccggtctga ttttttgtaa aagcttcagg atttttccat aatttccatt tgatgtcgga
    22321 tagattcttc gtgaatggca ataagaacgg ttttcatgaa ttcaccatcc attcccatct
    22381 caatggcttg ctccacacga ttgtgcaaaa tttcatcata tcgctgtgcc tgaagaatag
    22441 gcatgttgtg ttccttttta tatctgccaa tctcttcagc cacacgcatg cggtgagcca
    22501 gcaattcgag caaattgttg tccaattcat caatctgacg acgtaaagca gaaagatttt
    22561 cggttgtttg catggtgtcg cgaataacca gtgattctaa aataaattca agcacctgcg
    22621 gagtaatctg ctgggcagca tcgctccaag ctttttcagg attgcaatgc gattcaatca
    22681 ttaatccatc gaagtttaaa tccatagcct gttgcgataa tggagcaatc agttcgcgtt
    22741 ttccgccaat atgactcgga tcgcaaaaaa taggcaaatc aggcatttgt cggcgcaatt
    22801 caatggggat gtgccattga ggcaaatttc tgtaaatttt tttgtcgtac gaactgaaac
    22861 cgcgatgaac agcggctatt ttgtgaattc cggcattata aattctttct atagcaccta
    22921 tccacaactc caaatcagga ttcaccggat ttttaataag taccggaata tcgacgcctt
    22981 ccagcgcatc tgcaatttcc tgaacagcaa aaggattagc tgtagttcga gctccaatcc
    23041 aaagaatatc aatatcgtgt gcaagggcag cacgaacatg tttggcgttg gcaacttcca
    23101 cagtggtgta caatcccaat tccgatttca gtcttttcaa ccacggaaga cctttctctc
    23161 ctactccttc aaaaccgccg ggtttggtac gaggtttcca tataccggca cgataaattt
    23221 ttataccgat agaagccagc ccgcgcgctg tttccataac ctgttcttcg gtttcggcac
    23281 tgcaaggccc cgctatgaca atgggacgtt tggcttcgat tcccggcagt aaaattgatt
    23341 caaatttcat aattttgctt gttttaataa gtttacaatt cctttttcta aaaaatccat
    23401 atcaatgttc gattcttctt accacttcct ccagtgtagg ttccggacaa cataaagaaa
    23461 tgcgaacata acgatttccc ttgtctccaa aaataaatcc gggcgtaata aaaacgtttt
    23521 tcccatacaa aatttcgtcg gccaattctc cggcgtcttt gtaacgatca gggattttac
    23581 cccaaacaaa aagtcctacc tgccgagtat cataagtaca tccgagtaaa tccagaattt
    23641 taaaagcaat gtttctgcgt cgtgcataaa caacatttat ttgatcgtgc cattcctttg
    23701 gatttttcaa agcttcggcg gcggctatca tcatcggacg gaacattccg gaatcgacat
    23761 ttgatttaac tctcaatacc cattccacaa attggcgatt ggaagccagc atggcaatac
    23821 gccaaccggc catgttgtgc gacttgctca tggaattcat ttcgatgcaa atatctttgg
    23881 ctccgtcaac agacaaaatg ctcaactttc ggtcattaag aatgaaactg tagggattgt
    23941 cgttacaaat aataatattg tgctttttgc caaaagccac cagtttttca aaaagttcca
    24001 cagaagcatt ggctcccgta ggcatgttgg gataattggt ccacattaat ttcacacctt
    24061 ccagattcat tttttccaat gcatcaaaat cgggctgcca ttcatggttt tcgtctaaat
    24121 cgtagggaat aacttttgct tcgcacaaat tgctgacgga agtataggtg ggataaccgg
    24181 gattgggaac gagtacccca tcaccgggat tgagaaatgc caacgaaatg tgtagaattc
    24241 cttccttcga gcctatcaac ggctgaattt cggtattggg attcagctct acgccaaacc
    24301 attttttgta ccattcggca aaaccgttgc gcaattcggg aatacctata taactctgat
    24361 aaccatgaac atttggcttg tgcgcttcct tgcaaagtgc ttcgatagta gcttccgaag
    24421 gaggaagatc cggacttccg attcccaaat taatcacatc tttaccggcg gctttcatct
    24481 cgtcgatttc tttcagtttg gtcgagaaat aatattccga aacattattt actcgcctgg
    24541 caggttcaat ctccattttt aattgtttta aaattataat agttgagtta taaataagct
    24601 gttaattttc cgcatgatat tcccccatta tttgaaattc gtcgattaag ggaagaatgg
    24661 catcaatggc ttgtttatat cgcgaataat cattaaatgt caggttgata taaaattgat
    24721 attcccattc ttttccaatg atcggcaaag attgaatttt ggtaagattc atgtgataga
    24781 aagcaaaaat agagagaacc tgcgaaagac ttccctctac gtgcggcaat gtaaaaacca
    24841 gagaagcttt gttgatttca tcgtttttgg tcatttccaa cgccagtgtc ggttcagcaa
    24901 caataaggaa acgggtaaaa tttcgtttgt tggtttcaat gtttctctcc aaaatttcca
    24961 atccatattt ttcggcggca aaaaggctgc aaattgcagc tcgcttataa atctgaccat
    25021 cagcgatttc tttagccgac aaagccgtgt cttcactttc taccgctttc aaatgcttgt
    25081 gagattccag gaagtcttca cattgcatca aagcaatggg atgcgaatga acttcttcaa
    25141 tttcttcgag cttctgatga ggcaatgcca ccaaatgatg ctcaatgtgc aacttttgtt
    25201 ctcctacaac aagacagccg ctttcgcgta aaagattgtg attttgcaac agactcccgg
    25261 caatggtgtt ttcaatggca ataattccca aaagtgattt atcggcctct atggctttaa
    25321 aaacatcctt aaaggtctca cagggaataa tctcaatttc ttctccctta aaatactcac
    25381 gcgctgcaat ttcgtgaaat gctccactga taccctgaat ggctactctt ttcattttat
    25441 atgccttaaa ataaaaaatc ctgctcgatt gacttgagca ggattctata ttgttttaaa
    25501 aatttcattt tttcatgcca tttttttaca tatcattatc ctgctcttac cttttaaagt
    25561 aaaagtaaaa ataaaaatat gtaaagaaac ggcttttcat aaaaaatatc ttgaagtgtt
    25621 tttgtttaac ggtgcaaaag taaaaataat tttgagttaa aacaaacaaa ttgataaaaa
    25681 agtttcaaaa aataatgaat ttgttattta gacggaaatt catcaataaa tttcagcgct
    25741 ttactcatgg cagccgaaag ccctgcagca ttctttccac cggctgtggc aaagaaaggc
    25801 tgtcctcccc cgcccccttg aatttccttt gcaatttctc gaattatttg agcagcattc
    25861 aatcctctgc ttaccaaatc gtcagaaagt agcaatgtta acgaaggttt gccatccatc
    25921 acattgcctg cgatgaaaaa gagattttcg ttctgcagat ttttaatctg gaatgccaac
    25981 gttttagcct gatcagcagt caaatttcct tcgtagcgaa caacatgaat cccatttgtt
    26041 atcttcgctt cagccataag ccggtcgcga agttgaagca atttttcctg ttgaaaggtg
    26101 ttgacctgtt ttttcagttc cgtattttct tccatcagct ttctgacggc gtttaccaaa
    26161 ttaccgggat tgttcaatat ttctctcgtt tccgaaagaa tgtcctgctg ctcatccatc
    26221 aatttttcaa cagcttttcc tgttatggct tctattctgc gaattcctgc cgcaatggaa
    26281 ctttcggaaa ttattcttac cattccgatt tctccggtgg cttttacgtg cgttccgccg
    26341 caaagttctt tagaactgcc aaactgaatc actctcaccg tttcgccata tttttcgcca
    26401 aacaatgcca ttgcacccat ttcttttgct tcgctaacag ggacttcccg catttcgtta
    26461 agtggaagat tttcacggat tttcctgttg gcaagcgctt caacggcacg aatttcttct
    26521 ttggtcattt tttgaaagtg ggaaaaatca aaacgcaagc tgtccggtcc aacatacgaa
    26581 cctttttgct ccacatgcgt tcccaaaact gttctcagag cttcgtgcag caagtgagtt
    26641 gccgaatgat tacaggaagt ggcttgtcgg ttttcaagat gtacaacggc tgtgaaagtt
    26701 cccgtaattt ctgacggaag ctgtttggca atatgaaccg caagattatt ttcctttttg
    26761 gtatcaagaa tttctatttt ttcgttttca cccactaacc agccgctgtc gcctacctga
    26821 ccacccattt cggcatagaa tggcgtaaca gacaaaacaa gctgataaaa ttcctgattt
    26881 ttctgtttta ttttacgata acgaagtatg ttcgtttcgc acgaagtgca atcgtagcca
    26941 acaaagtggc tgtcgccttc acgcaaaaca atccaatcgc ccgtttcgat attggctgca
    27001 tttcgggctc gctctttctg cttttgcatt tctgcatgaa actcgttcat gttgacggta
    27061 aaattatttt ccctcaaaat gagctcggtt aaatcaatgg gaaatccaaa agtatcgtac
    27121 agaacaaatg catcggcacc ggaaatttct tttttgtttt ccaattgagc ctcttccatt
    27181 tttctttcca gcaatcggat accggtttca agcgtacgta aaaaagattc ttcttcttcc
    27241 tttattactt tttcgatcag tgtcttttga ctgatcagct ctggataggc tttccccatt
    27301 gtttcgcaca gaacgtcaat caaccgatac ataaaaggag cgtgttgatg cagaaaagta
    27361 tatccgtacc gcacggctct gcggagaatg cgtcgaatga catatccggc tttcatgttg
    27421 gaaggcagct gcccatcggc aatggaaaag gcaattgccc ttatatgatc ggatacgaca
    27481 cgcatggcga tatcgatttt ctcgtcttta ccgtattctg taccactgat tttccctatt
    27541 tcgcgaataa tgggttgaaa aatatcggta tcgtaattgg aagtttttcc ctgaataacc
    27601 atacacagac gttcaaatcc cattcccgta tcgattactt tggcgggaag ttcttccaaa
    27661 gtgccatcgg cttttcggtt gtattgcatg aaaacattgt tccaaagctc aatcacgagt
    27721 ggatgtccct gattgataag cgaaagcccg tcaagttttg cgcgttcttc ctcgctccga
    27781 atgtcaatgt gaatttcgga acacggtcca cagggccctg tttctcccat ttcccaaaaa
    27841 ttgtcttttc tgctgccatt gatgattcta tcggcaggca gaaattgcga ccaaatggcg
    27901 gcagcttctt catctctttc gagcccttca gatggagaac cttcaaaaac agttacatat
    27961 aatctgcttt tatcgatttt caacacttcg gtgagatatt cccatgccca ttcgatggct
    28021 tctttcttga aatagtcgcc aaacgaccag ttgccgagca tttcgaacat ggtatgatga
    28081 taggtatcgt gcccaacctc ttccaagtcg ttgtgttttc cactcacccg aagacatttt
    28141 tgcgaattgg ctacgcgtgg atattttacg ggatcattgc ccaaaattat gtttttaaac
    28201 tgattcatac ctgcattggt aaacatcaag gtaggatcat ctttgataac cattggtgcc
    28261 gaaggtacaa ttttatggcc tttctctgcg aaaaaatcaa gaaaagattg tcgaatttcc
    28321 tgtgaagtca ttttgcgaaa ttaattaaat ccattttcct ttgagtgaat gcaaatttta
    28381 ccgaaggtta ataatctcaa ataaagtctg caaaaatacg ccaaatttat tatttttgca
    28441 ctatcatgct tgataaaaag ttgaatttgg ctcatttatc tattcttttt tcaaaatttt
    28501 agcgcttttt ttatgttcct aaatcgtttt ctatggcaaa gaaaaaatat catttcaatc
    28561 ccgatacttt aagttacgaa ccgatagaaa attcactgtg gttcaaaata cgtcgttttt
    28621 tgtttcacgc ttttaccgga attgcaacag gaacggtttt tttcttcatt ttggcttcaa
    28681 cagttcaatc tcctgaagaa agagaattga atgcagaaaa aaacaaaatg gaggctcaat
    28741 acaaagtgct tcaacgccag ttcaatgaag tacaggatgt tttgaccgac ttggaagagc
    28801 gggacaacaa cttgtatcgt gttgtacttc aagctgaacc gattccgttt accgtaagaa
    28861 aagccattgc atcgaatacc gattattaca accagcttta cgacatgacg aattctgaaa
    28921 ttgtagtcag cacaaccaaa atgctgaata ccctgaaaaa gcagctttat attcaatcgc
    28981 gttcttacga cgaattggta acccttacaa aaaacaggga aaagatgttg caatgtcttc
    29041 ctgccattca gcctgtgctg aacaaagatt taaaaagaat ggcatcaggt tatggttggc
    29101 gcatcgatcc tgtttaccat acgcgtcgct ttcatgaagg gatggatttc gttgctccta
    29161 caggtaccga tgtgtatgca accggcgatg gcactattgt aaatgcgggc tgggaacagg
    29221 gttacggaaa ctgtatagta attaatcacg gatttgggta cgaaacacta tatgcccatt
    29281 taagcaccat caaagtgaga ccgggtcaaa gtgttaaacg aggagacatt atcggactgg
    29341 taggcagtac gggcaaatca actgctcctc acctccacta tgaagtacat tataagggac
    29401 aggttatgaa tcctcaaaat tattatttcc tggatttgtc gcctgaagaa tacgacagaa
    29461 tggtacaaat gagcaacaat ttcggagaag ttctggatta ataaaccatt ttttgaaaaa
    29521 tttcacagag aatgaatgaa ttacaaggaa acaaaaatta ttcgcttacg aagttggaat
    29581 tatcatcgtt gcccggtccg atcggcgtgt tcgattcggg atatggcggc ttgaccatat
    29641 tggaaaaaat ccgtcaaagg cttccccatt atgattacct ttacttgggc gacaatgcgc
    29701 gcactcctta cggaactcgt tcttttgaaa tcgtttataa atatacgctg gaatgtgtcg
    29761 ttcggctgtt cgaattgggt gcccatttgg tgattttggc ttgtaacaca gcttctgcca
    29821 aagcattaag aaccattcag caaaatgatt tgccgaaaat tgatccccgc aggcgcgtac
    29881 tgggaattat tcgacccaca gtagaaataa ttgataaact gacacacaat ggtcatgttg
    29941 gattattggc cactccgggc actgttcagt caaattccta cggactcgaa atacaaaagt
    30001 tgtacggaaa tgctattacc ctttcttccg aagcttgtcc catgtgggta ccgctcatag
    30061 aaaattttga acacaattct cccggagccg attatttttt tcaaaaaaat atcaatcact
    30121 tgttacaaac cgatccacag attgatacgg taattttggg ctgcactcat tatactttgc
    30181 ttcaggataa aattaaaaat cttcttccgc ccaacatcaa tatcgtttcg cagggagaaa
    30241 ttgtggcgtc aagccttgat gattacctga accgacatcc cgaaatcgac gcactttgca
    30301 ctaaaaatgc ttcctgtcgg tttctgacaa ccgaatcggt ggaaaaattt tcggcttctg
    30361 ctacaatttt tctaaacgaa cccgtactgg ctgaacatat tgacatcgat taacagcatt
    30421 tctgtttcaa ctctattaaa gatttttcat ggaagatgaa cgatttgctg ggaaaacaat
    30481 aatttcatta accggaaatt aatttttatg ccgatttttc ttttgaaatt caatgattta
    30541 acttgtgttt taaaacaatg ttttataaca taaaaatttt atctccaaat taatggactt
    30601 tttatacctt gtcgattcga tttttacaaa gttaaaacat aattttatag tattaatcaa
    30661 ttaaaaatta ataagttatg aaagtatatc agcctaatga aataaagaat atttctttat
    30721 tgggaagttc tgggtcgggt aaaaccaccc ttgtggaagc tatgcttttc gagggtggaa
    30781 tcattaaacg cagaggcacc attgccaatc agaacacagt atgcgactat tttccggtag
    30841 aaaaggaata tggatattcg gtattcccaa cactggcaca gattgaatgg cgtcaacata
    30901 aacttaacat cattgattgt ccgggttctg acgattttat aggtgctgtg gtaacatcac
    30961 ttaatgttac cgatatggga ttaattgttg taggagctca atatggagtg gaagttggca
    31021 ccaacaacca tttttcttat gccgaaaaat acaacaaacc tgtcatgctt gttgtgaatc
    31081 agttggatca cgacaaagct gattatgaac atacccttga acagctgaag gaaaattttg
    31141 gaaataaagt tattcagctg caatatccat tacagacagg aagtggattt aattccttgg
    31201 ttgatgtact gcaaatgaaa atgcttcgtt tcaaaactga aggtggagca cctgaaatta
    31261 ccgaaattcc cgactccgaa aaagcaaaag ctcaagagct tcacaatagt ctgattgaat
    31321 ctgcagcaga atttgatgaa agtctaatgg aaaaattttt tgaagaagga atactttcgg
    31381 atgaagaaat ttataaaggt ttgtataaag gtgtgttgga aaggggcatt tttccggtaa
    31441 tttgcgtttc tgccgccaat gatgccggtg taagtacttt gatggattat ttggtgcgga
    31501 ttgctccctc cgtttccgac agcaaacgcc ccttgactaa agatggaaaa agtgtagatc
    31561 caaatccaga tgggccaacg agtatctatg tttttaaaac aaacgttgag ccgcatattg
    31621 gagaaatttc tctctttaaa gttatgagtg gaaaattgaa ggaaggagat gacctttaca
    31681 acgtaaaccg ccaagtaaaa gaaagaatca gtcagatttt ctgcgttgcc gggcaaattc
    31741 gcaacaaggt ggaagaaatt tcagccggag acttcggagc aacggtaaaa ctgaaggata
    31801 cccgcacagg aaacactttg aacggcaagg gttgcgatta tgactttgtc ggagtagaat
    31861 atcccgaacc caaataccgt cgggctatta aagccaaatc ggaagctgat gaagaaaaat
    31921 tgaacgaaat actcactcga atgcacgaag aagatcccac atggatagta gagcagtcga
    31981 aagaactgaa acaaaccatt ttgcatggtc agggagaatt tcacttgcgc accttgaaat
    32041 ggagagtcga aaacaacgac aaaatagaaa ttgaatttaa agaaccaaaa attccgtatc
    32101 gcgaaaccat aaccaaaaaa gcccgagccg attaccgtca taaaaagcaa tccggcggtg
    32161 ccggacagtt tggggaagtt catctaattg ttgaaccgta tttcgaagat gttcccattc
    32221 aggatgtgta tcgtttcaat ggtcaggaat acaaaataag cgttcgtgat gttcaggaaa
    32281 tagatttgga atggggcggg aagctggtat ttgtcaacag tattgtaggc ggtgccattg
    32341 atgcacgatt tttaccggcc atcctgaaag gtgtaatgga tcgtatggaa caaggaccac
    32401 taacgggttc ttatgcgcgt gatgttcgcg tcattgttta tgacggaaaa atgcatccgg
    32461 ttgattccaa cgaaatttct ttccgattag ccggaagaaa tgcttttgca caagctttca
    32521 aggacgccgg ccctaaattg ctggaaccgg tttatgatgt ggtggtaatg actccatcgg
    32581 acagattggg agatgtgatg agcgatttgc aaagccgtcg tgccgttatc atgggtatga
    32641 acagcgaaaa aggttttgaa aagttgcaag caaaaattcc tttgaaagaa ctgagtaatt
    32701 attccacaac cttgagttct ttaaccggtg gaagagcttc gtttgtaatg aaatttgcaa
    32761 gttacgaact cgttcctatc gaaattcagg aaaaactgtt gaaagaatat gaagcttcta
    32821 agaaagaaga agattaactt ctaaattgaa ttttaaaaaa aggggcaatt tactttgaaa
    32881 gtagattgtc cctttttagt attataattc aattgttgga ttaaccgtaa ataattttgc
    32941 catcgtgagt ttgacttcct tcaaagcttt tgttgtactg ctgcaatgca cgataactca
    33001 tacccatgct cgaaaagcct ccatcgtgga aaaggttttg catggttacc ttgcgtgtca
    33061 ggtcgctaaa cattactaca caataattgg cgcaatcatc tgctgaagcg tttcccagtg
    33121 gcgacatgcg ttccgaaaaa tccattaaac tgtcaaatcc cttcactccg cttccggcag
    33181 tagtaggagt tggcgactga gaaatagtgt tgatacgcac attgtgttct cttccgtaaa
    33241 tgtatccgaa gtttcgggct atcgattcca gcatggcttt ggcatcggcc atatcgttgt
    33301 atccatacat cgttcgatga gaagcaatgt aggaaagagc aacaaccgaa ccccactcat
    33361 tgatggcatt catttttttg gcagtctgaa gcattttgtg aaaagatatg gctgaaatat
    33421 ccaatgtggt gtgcatcaaa tcataatcca aatcatcata gggcctcttt ttcctcacat
    33481 ttggtgacat gccaatggaa tggagaataa agtcgatttt accacccaaa atttccatcg
    33541 attgagagaa aacttgctcc aaatcatcaa gattggtagc atcggcgggg ataattttgg
    33601 cattgatttt ttctgccaat tcatttatgg ttcccatacg tatggcaatt ggcgtgttgg
    33661 acagggtaac ggtagctcct tcttcgacaa cgcgctcggc aactttccag gcaatcgaca
    33721 tatcgttcag agctccaaag atgattcctc tttttccaac taataaatta ttactcatag
    33781 tctttaattt taatcgtacc gggaaaaatt gaaaataaac cggtatgaag gttgttaatg
    33841 ataaataagt tggctgcaaa agtaatcaaa ttgttacatt ttagcaattt tgattttgaa
    33901 agtgatcttt ttataattgt aaatacctat aaatgaattg attgaataaa attagcgccg
    33961 taagaacttc caactctaaa aatagataat ctaaattatt tgctcaaaat aaaatcacat
    34021 cttctattca ttctataggc tctgggaggt tgagggattt ttccctttcc attggaaata
    34081 attctctgcg gattgatgtt atgggtgaaa accaattccg cttttacgcg attggagcgt
    34141 tcctgactta atcttttgtt gtatttgtta cttcccacat tatcggcata tcctctgatt
    34201 tctaccaata aggttggatc agaatttaat ttttctgcca gtttttcaat ttctattaat
    34261 gcttcttcat ccaaattggt tttgtcaaag tcaaaataca cagaggcaaa agcgcatggt
    34321 ttatcttgag ctatgcttct accccaaaaa tcgacacaca gattttttgg tgaattaggc
    34381 tctttgtctc ggttgtcggg gacaccatct ttatcttcat cttttgttga agcacttata
    34441 tattgaagat attcaggatg aatctctttg gtttcgggga ttacttttat ttcaggaatg
    34501 ccacgtccgt tataatcaat gtaaagcccc tttggtgtat tgggctccaa atccctgcta
    34561 ttggggacgc catcgccgtc atcatccggt cccggttgca aacttaattt caacttatcg
    34621 agttcttgag ccagcgaatc tgttttgatg tttgcccgat ttgctaaagt gaaaacatct
    34681 cctaaagtaa ttcttgtttc gttgttgcgt atgtgatttt tactttttgc tccaattttc
    34741 caacgcaacg aaagagctgc cattgcaata ttgtcgttgg taacaccttt aaaattatat
    34801 cctccggctt cttctttcaa attggttcct tcaaagttat ctttattctg caaacgatac
    34861 tgcacttttg cacctatagc aaatagtgac gagatattat actctatcaa acctcctacc
    34921 gggaaaactg cagcccatcc atctcttatt ctgtcaaact caatgccatt tttatacaaa
    34981 gtagaattgt aataggccac tccagcgcct accgtagcat aaatattcca tttggaatga
    35041 ttattttcat tacataaatt aagcagattc aacgaaaggt aaggagtgaa atgaaataca
    35101 tctgcgttga attttttggc agcatcttct gcagaaacgg gaaaataaaa aaattcagtt
    35161 ccaatgcccc aaaaaggact aagagtaagc tctgttgaaa atccaaagct tgcctttgct
    35221 gcagaattcg gtataatctg gttataattt tgattattgt ctccgtcaaa attattgatt
    35281 cctgcatcca aggttattga ccatcgcctg taatcatttg gttgtccata agaggagatg
    35341 aaaatggaca aaatgaaaaa gccaacaata aattttttca tggttataag atttattcca
    35401 actgaattta gaatttaata ttaaaacttg tattcgcaac aaataaaata acttatgcaa
    35461 aactacaaaa ataatattaa atttccaaaa acaaaataat tttaatcact attaaccgac
    35521 aaaaatcaga atttgtcaat tttaattttt aaataattga ataatagttt attaatatgt
    35581 ttttctctgt tgactatagt cagtttctgc gaaagcatag atttctccat cacaaatgaa
    35641 aaatctttac aatccacccg actcattccc caatccatgc gttttcagga atggagagaa
    35701 taaggatagt tcttgtcaaa tttttcttca gcttatttga tttttctgcc taaatgacca
    35761 tatttcagcc agttgaaatt gccaaaatga ctaatttcca cgtttggtat ttattttgtt
    35821 tttaacttac gagcaaaaaa tatgtttaac taatcaaagt gatggagggc tgatatatgg
    35881 caaccaacag actaaccgaa caattaccat caaattttga ccgttatttc gataacgaat
    35941 gggaagatta tttctggact gacagggaat tggcggcaaa tcatactact ttaccggcag
    36001 ttaacatcaa agaaaatggt gaagctttcg aaattgatgt ggcggcaccc ggttacgaaa
    36061 aaagcgatat taaaatcgaa atgaacgaag atttgctgac cgtttcttcc aacaaaaaac
    36121 ttgatgaaga aaaaacggaa ggtgaatata caaagcgcga gttcagttat cagtcgtttt
    36181 tacgaacatt tactctaccc aagacagttg atgcagaaaa aatttcggca gtatatgaaa
    36241 acggaatttt gaaaatcacc attccgaaaa aagaagaagc caaagtaaag ccggcgcgga
    36301 cgattgatat tcaataacaa aaggaaaaaa ttagattttt tagatcagct tttttactat
    36361 cgaaacaaga aagaaaaata tatggaaaca tttcaggata ttatcaacgg agataaaccg
    36421 gtattggtag atttttttgc gacgtggtgc ggaccatgta aagcgatggc tccggtgatt
    36481 gaaagtctgg gcaaagaagt tcaggggcaa gcccgtgtgc taaaagtgga tgtggacaaa
    36541 aatcaggcat tggctgccaa atatgggatt caggcagttc ctacctttat gctgttcaaa
    36601 aaaggaaatg tcatttggaa acattccgga gcaacagata agaacacctt gatgcagcaa
    36661 ataaaatctg ccgtataaca agatcaaaac aattgtggaa ggaaggtaaa aacattttgg
    36721 agtacaaaat actcttctat ttctcaaaat gtgtttacct ttttctttta aaaaaatcct
    36781 gcatcaatgt agcacattct tctgccaaca ctccagacac tacttctgtt ttagggtgaa
    36841 gagcatctgg agcaaatttt gaatatccgc gtttttcgtc ggaagctccg taaacaattt
    36901 tcttgatttg tgcccatccc agggctccgg cacacataac gcaaggctct atggtaacat
    36961 acagggtaca gtcggacaga tatttacctc ccaaataatt ggcagctgaa gtaatagcct
    37021 gcatttcggc atgagccgta acgtcattca aagtctcggt gagattgtgg gcacgagcaa
    37081 ttatacttcc ctgacataca atgacggcac ctatgggaac ttcatctttt tcggctgctc
    37141 gccgtgcttc cgcaagcgct tgtttcatat atttaatatc ctgattttct tccatttgtt
    37201 tatcctttca ataaaatatg aattttgaaa gcaaaggtaa aaaattttct tcccgtttca
    37261 attcagatag accgtttgat aatttttgct cttgataatt ctatgagttt tagatttaac
    37321 aaaaaaaccg aaattttatt cgtcggaatt tttgtaattt tgtaatttga aaaacttaca
    37381 tatgaaaatc ataattgtcg gcacagccta tccttaccga ggcgggttgg cttcgttcaa
    37441 cgagcgtttg gcgcgtgaat acgccagaga aggtcatgaa gtggaattga tcactttcac
    37501 tcttcaatat ccaaattttc tgtttccggg aaaatcgcaa tattcaacgg aaaaaccacc
    37561 cgaagacctt aaaattatcc gccgtatcaa ttcgataaat ccatttaact ggataaagat
    37621 tgggaaagaa atcaagaaga aagctcccga tttggtcata ttttgctact ggacttcttt
    37681 tatagcaccc tgttttgcaa ctatcgcacg atatgcgcgt tctccacaaa ctaaaatgct
    37741 cggtttggtt cacaatatga ttcctcatga agcaacacca ctggacaagt ggctgccgcg
    37801 atatttcgta aaaaatatgg atggttttct ggcaatgtca aattcagtga aagaagatat
    37861 tcaacatttt gacaaaaaaa ataaaccaaa aattgttgtt cctcatcctg tttacgacca
    37921 ttacggtgca attgtatcga aaagcgaagc tgcattgaaa ctcggcttgc ccccaaaaaa
    37981 aagatatgtg ttgtttttcg gattcattcg ccattacaaa ggcttggatt tactcctgga
    38041 agctttttcc gataaaagat tgaaagatat ggaagtgcaa ctggttgttg ccggagaatt
    38101 ttacgaaaat cccgagcctt atttaaaaca gattgcacaa ttgggactgg ccaacagagt
    38161 tgaactaaga accaatttta ttcctgaaaa tgaagttaaa tactatttca gtcttgccga
    38221 tttggttgca cagccctatc gttcagccac tcagagtgga gtgagccaaa ttgcctatca
    38281 ctttgaaaaa ccaatgttgg ttaccaatgt gggaggcttg gccgaaatta tcccaaatgg
    38341 aaaagttggc tatgtagtaa acggacaacc caaagaaatt gccgacgcca tagttgattt
    38401 ttttcagaac aaccgggaag aaatattttt gcccaacata aaaacagaaa agatgaaata
    38461 cggttgggat aaaatgactg ctgccatcaa caaacttttt ttcaagctct gaaaaatttt
    38521 tcccctgccc cgacttttca taatttcaat tagtagaaca tagagaaaaa tatagagaaa
    38581 taaattgccg atatatgtct gaaccaattg tagaaacgcc tctaatgaaa cagtatttcg
    38641 agatgaaatc aaaacatccc gatgccattt tattgtttcg ttgtggagat ttttacgaaa
    38701 ctttctcaac tgatgctatc gaagcttcgg aaatattggg aattacactc acgcgccgtg
    38761 ccaacggatc ggcaaaaagc gtagaattgg caggatttcc ccatcatgcg ttggatactt
    38821 atctgccaaa attggtgaga gctggaaaaa gggtagccat ttgcgaccaa ctggaagacc
    38881 ccaaacttgc aaaaaaaatt gtaaaaagag gcatcaccga actggttacg ccaggtgtta
    38941 cttacaacga caccatcctc aaccacaagg aaaacaattt cttagcgagc gtttacctca
    39001 acaaaaatca ggcaggtgta gcctttcttg atgtttctac cggagaattt ctgttggctg
    39061 aaggaacaaa cgaatacgta gataaattgc tgaacagctt ctctcccaaa gaaattattt
    39121 tcgaccgtac caaaaagaaa gaatttgaaa atttatttgg aaccaaattt tattcctatc
    39181 cgctcgaaga ttgggtattt acccttgatg cagccaaaga caggttgctg aagcagtttg
    39241 aaacgaaaag tttgaagggt tttggtgtgg aaggacttgc caacggagtg gttgcagccg
    39301 gcgccattct ttattacctc gacctgaccc atcatcaaca aacgggacat attacccaac
    39361 tctctcgcat tgaagaagac cgctttgtat ggcttgacag attcaccgta agaaaccttg
    39421 aactttttga agctatcaac gaaggaggaa aaaccttgct cgaagtgctg gataaaacca
    39481 tcagcccgat gggcagtcga ctgctgaaac gttggatggc ttttccgttg aaaaatgtaa
    39541 aatctatcaa cgaaaggctg gatgtagtag aatatttttt caaacatccg gaattgaaag
    39601 aagctttggc tcagaatatt gcgctgattg gcgatttgga acgtctggtt tctaaaattg
    39661 ctgtgggcag aatcaatcct cgcgaagtgg ttcaattgaa aacggcgctc aaagccatcg
    39721 aaccaataaa acaattgtgt actcatacag aaaacatcac tttgcgcacc attgccgacc
    39781 agctgaatgc ctgctccatt atttccgaaa aaattgaaag agaaattcaa aacgaccctc
    39841 ccatactcat taatcgggga aatattattc aaaaaggtgt aaatgccgac ctcgacgaat
    39901 tgagagaact ggctttttcg ggaaaagatt tcctgcaaaa gattcaggaa cgtgaaagcc
    39961 aagccacagg aattccaagt ttaaaagttg gattcaacaa tgtttttggc tattatctcg
    40021 aagtacgaaa tacctacaag gaaaaagttc cagaaacatg gattcgcaaa cagacactgg
    40081 taaatgccga acgctacatc accgaagaat taaaaaaata tgaagaaaaa atactcgggg
    40141 cagaagaaaa aattattgat attgaaaccc gtttgttcaa cgaactggtg ttaagtcttt
    40201 ccgaatacat tcctgccctt caactcgatt cgaacttgat agcccagctg gatggtctgc
    40261 tttcctttgc gaaagtttcg gaagaaaacc gttatgttcg ccccgaaatc aacgatactg
    40321 aagtgctgga aattcatcag ggcagacatc cggttatcga aagacaactt cctttgggcg
    40381 aaagctatgt ggccaatgat gtgtatctcg acagctcgaa acagcaaatc atgattatta
    40441 ccggccccaa catgtcaggc aaatcggctt tacttcgaca aacggctttg attactctta
    40501 tggcgcaaat gggatgcttc gtgccggtag aaagcgccaa gattggcgtt gtggacaaga
    40561 tttttactcg tgttggagcc agcgacaaca tttctttggg cgaatcgact tttatggtcg
    40621 aaatgagcga agccgcgagt attctcaaca atctttcctc tcgcagtttg gtgctttttg
    40681 atgagctggg aagaggaaca agcacttacg atggcatttc cattgcttgg gcaatagttg
    40741 aatatattca cgaacatcct gctcacaaag caaaaacgct ttttgctacc cactatcacg
    40801 agctgaatga aatggaaaaa tccttttcgc gggtgaaaaa ctacaatgtt tccgtaaaag
    40861 aagtggacaa caaggtgatt ttcctccgaa aactggtgcg aggtggaagt gaacacagtt
    40921 tcggtattca tgtggctaaa atggccggta tgccgcaaag catcgttaaa cgcgctgaac
    40981 aaattcttaa acagctcgaa acggataacc gacaatccga tatttccaaa ccggtagata
    41041 aaattgccca gcacagggaa ggttatcaac tcactttttt ccaactcgat gaccctgtac
    41101 tcgaacaaat tagagatgaa atcaagaatt tggacataaa caacttgact cctatcgaag
    41161 cccttaataa actgaacgaa atcaaaagaa ttattatggg aaaataaaat ttatctgttc
    41221 ataaccttcg atgaatttta aagataatca taattggttt tttaaaaaat ttcatcttga
    41281 ttattgactt ataattctaa atttatgagt ttttaaaaca agtgttttta tttgctattt
    41341 taaactaacc tgttccaata tatctacaac gtcttcacga gtaattcttc taaaatgttc
    41401 ttcaacgggt gtaataaaaa taccgctcat tt
//
LOCUS       DAOSCH010000007        40776 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_734_length_40776_cov_6.367722, whole genome shotgun sequence.
ACCESSION   DAOSCH010000007 DAOSCH010000000
VERSION     DAOSCH010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 40776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40776
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_734_length_40776_cov_6.367722"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PLZ54_01290"
     CDS             complement(<1..220)
                     /locus_tag="PLZ54_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HOJ65608.1"
                     /translation="MSKLPHFFAVDLGATSGRTILGTLQNNRLDLKELTRFQNKIICI
                     HDKYYWNIYSLFEEIKKGLLTASQQGVHI"
     gene            534..1448
                     /locus_tag="PLZ54_01295"
     CDS             534..1448
                     /locus_tag="PLZ54_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ65609.1"
                     /translation="MKNIEAKNSILCRYLIANEQDTRWGLTITTVGKQSLSINEDYPP
                     KDGHPMGYLFSTEQGRVLDEYQLLYIVKGSGIFTSTSCKLTNINAGNMFMLFPGEWHN
                     YKSNPATGWEEYWIGFKGENIDKRVGNGFFCKENPVYKVGVLDEAVQLYNQGILVAIE
                     QKSGFQQMLAGIVNHLLGLAFCLNKYNEFENLEITNLINEAKIRFMEDFRKDIDIKDV
                     AKGLNMSYSTFRKVFKEYTGFAPAQFLQEIRIQHAKELLTNTNLNIKEIAFESGFENP
                     EYFFTVFKNKTGYTPIQYRNFTQEKISI"
     gene            complement(1653..2774)
                     /locus_tag="PLZ54_01300"
     CDS             complement(1653..2774)
                     /locus_tag="PLZ54_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOJ65610.1"
                     /translation="MPSIVIDSYIPYLRGVLDEVLDVTYLPHSQITNETVKNADALIV
                     RTRTKCDARLLSGSKVKFIASATIGYDHIDAAYCAENGIKWTNAPGCNALSVAQYMAS
                     VFSYVNRNFFELAGKTLGVVGVGAVGSKIVQLGASFGMNVLQNDPPRARKEDPYQFVS
                     LSEICSTSDIISFHTPLNMEGEDKTFHLADKKFFDTLKKKPVIINAARGEVISTSALL
                     DALNRQGILTAVIDCWENEPDIDTELLNKTALGTPHIAGYSAEGKANAASQSVRSVSK
                     FFGLGLDNWEVSNLPEPTKVDLSFVNNIEDFFLKTYNIESESNQLKNHPRLFEYLRSH
                     YPFRREPDAYLPQLNEIMQKQLIEQFPLFFNQKNFKTLA"
     gene            complement(3078..3254)
                     /locus_tag="PLZ54_01305"
     CDS             complement(3078..3254)
                     /locus_tag="PLZ54_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOJ65611.1"
                     /translation="MNTKEIVLEAMKKAGSPVNAGKIVEMTGLERKEVDKAMNELKAE
                     GAIESPKRCYWQPK"
     gene            complement(3313..4086)
                     /locus_tag="PLZ54_01310"
     CDS             complement(3313..4086)
                     /locus_tag="PLZ54_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOJ65612.1"
                     /translation="MKILILGAGKMGTFFTDLLCLHHEVALYDVDPQKLRFVFNTYRL
                     TQYKEIQEFEPELVINCVTLKYTIEAFKNVLPYLPENCMLSDIASVKTGLKEFYESTG
                     KRFVSTHPMFGPTFATLDNLSSQNAIIISESDYLGKMFFKDLYHSLHLKIFEYSFEEH
                     DKTTAYSLAIPFASTLVFAAVMKHQDAPGTTFKRHMDIAEGLLSEDDYLLTEILFNPY
                     AEEQIDNIRAQLKVLLDIIKKKDAARMQEFLEKVRKNIE"
     gene            complement(4266..6140)
                     /locus_tag="PLZ54_01315"
     CDS             complement(4266..6140)
                     /locus_tag="PLZ54_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ65613.1"
                     /translation="MKIKQTKNISPWAWVPTLYFAEGIPYVVAMTVAVIMYKRLGISN
                     ADIALYTSWLYLPWVIKPFWSPFVDLIKTKRWWITSMQLLIGAGFAGVAFLIPAPFFF
                     QATLAVLWLIAFSSATHDIAADGFYMLALDSSQQSFFVGIRSTFYRFAMIAGQGILII
                     IAGALESHTGMEPVVFSVQSSPDPISQIVNVDSLSKVNFPEDAEMKFVVFPASLSLST
                     QPITSDSLNKIKAAALENNKRYQFIAEENLQNNQTKKENDSWWHQHVSGPFADFIREN
                     FGEKREKISMVENLTGNAGVVAIRLTKKPEAGKPLILNLSQSKGDKSISLVSGERLVF
                     TDENWDKPALVVVQLDAKLKGQAVSEFKGVSGNIRLAWSITFFILSGLFILFYLYHRF
                     ILPRPASDHPSLTHSSKDIIREFGRTFATFFQKKGIGLAIAFMLLYRLGEAMLVKMSS
                     PFLLDPHEVGGLGLTTGQVGLVYGTVGVIALTLGGIVGGIAASRKGLKYWIWPMALCI
                     TLPNLAYLYLSWFMPENMLLINAAVAFEQFGYGFGFTAYMLYMIYFSEGEHKTAHYAI
                     CTGFMALGMMLPGMMAGWLQELIGYNHFFIFVLICAIPTLAIIPFMKIDKEFGKKKSV
                     "
     gene            6579..9353
                     /locus_tag="PLZ54_01320"
     CDS             6579..9353
                     /locus_tag="PLZ54_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOJ65614.1"
                     /translation="MRKIFFFLSLFFFPILIQAQTFSGSALITGKLIDKETKKPVPLA
                     NIRVLQQRDSSFVNGQASRNDGSFSIPVHYGSYIVHISFLGYDDVFKHVQVSASNPIA
                     VLDTIFLRQTDFKLSEVIITEKAPEVRVKGDTLEFNAGSFKVTESAVVEDLLKKMPGV
                     EIDKDGKITVNGREIKKIMVDGKEFFSSDPKVASKNLPAKMVEKVQVLDKKSEMAEMT
                     GFDDSDEETVINLVIKQGMKEGLIGNAFAGYGSKDRYEGNAMVNYMRNQNQFTFIGGL
                     NNTNNAGFTDLASSMFGNMGGRGMRGMFGNQDGVTTSGMGGFNFNTQLSQTFKLGGNV
                     RYGSTNSDVLSKIRTQNMLSSGNTFENENDTTNSFSQNFNMDLRMEWTPDTVTRIIFR
                     PSVGVYENSRDEISNFNTTNEENDSINKGDSKYSSDGNGYNVGGRLEVSRQLGKKGRV
                     LSLQLRGQLNDADNEGYNLSNTYYFGTKPDDIIDQKFTNTSNSKNWQSFLSYVEPIGH
                     NNFIQLAYNYQQKFSESNKDTRTKDDLGNYSVMDTTYSKRLENDFVNQSIEMNFKANR
                     GKYDYMFGFSMQPFNSSSKTYKGDSLYNNTSNQGINYAPSAQFNYRWSRQNNLRIRYS
                     GITSQPSVTQLSPVRDVSNPLNVTYGNPDLKPSFSHQLNIRYMNFMPQQKSSINFFGN
                     ASYTLNDIVSSTSTDVFTGQKETTYKNVNGNWNANGRLTLNLPIKKTNFSVFSMSSGS
                     YTHSKGYSNNEINLSQRTGLGQTLGIDFRSGVVDFGTRGNINYNNIQNSLTGQQDQEY
                     FNYNVSANTTIYLPYDLSIDSDLSYNTNSGYSTGFEQEALIWNASLQKQLFKHKNGSL
                     RLKIYDILQQKSNISRRATANYITDTTTNTLTSYFIVHFVYRFNIFKNGANQNDMMPH
                     RSFGGGPSGH"
     gene            9856..10101
                     /locus_tag="PLZ54_01325"
     CDS             9856..10101
                     /locus_tag="PLZ54_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOJ65615.1"
                     /translation="MNIYVGNLSYKVRENDLQGVMEEYGQVSSCKIIKDRETGRSKGF
                     GFVEMSDDTAATKAIEELNGAEFDGRTMVVKEARPKN"
     gene            10207..11085
                     /locus_tag="PLZ54_01330"
     CDS             10207..11085
                     /locus_tag="PLZ54_01330"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOJ65616.1"
                     /translation="MSSLKGVFKNRKIFSQLLLLLFLSGLFFYIGLAFWMVISAGKRE
                     DAQAIRVLQLIQSIGLFLIPPFIFAYLVSDRKPTEYLSLSQKINLKDVMYVILIMFIA
                     IPFVNFLTSLNQQLVLPEVLAPVEEWMKQTEEEAAVLTKQLLQVDNIGGLFANLILIA
                     LIPALGEELYFRGALQNVIGRGKGTHFAIWMTGFLFSAFHLQFYGLLPRLLLGVFFGY
                     ILVWSGNIWLPVIAHFTNNAIAVIFYYFSGQNNEVVNIDAVGTNDSWWMGVISLILTI
                     LLVFRLKSRIPKKQHI"
     gene            complement(11082..11477)
                     /locus_tag="PLZ54_01335"
     CDS             complement(11082..11477)
                     /locus_tag="PLZ54_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase"
                     /protein_id="HOJ65617.1"
                     /translation="MWVLIIILISVGLVLMTVTYFSRKGKREEATVQVRENSECCGAH
                     ALCERENLLNMDNEIEYYDDEELDALSGKSPKQFSDKEIEQLSEVFYSLQESDVAGWL
                     RSLQMRQIELPLFLREEALMIVGERRNAC"
     gene            11812..13302
                     /gene="nhaA"
                     /locus_tag="PLZ54_01340"
     CDS             11812..13302
                     /gene="nhaA"
                     /locus_tag="PLZ54_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HOJ65618.1"
                     /translation="MIDHHSKRARIYIFLRNFKWKTNPGIVMMVVAIATLIIANSPLK
                     EWYQSFWNIEFLLGTNSFNLFSHHGTPLTLLSFINDCLMAVFFFSIGLEIKREVLIGE
                     LSSFRQSILPMIAALGGMIVPVLCFFVTGRIQNLSPDEMRGMFIPMSTDIAFSLAVVS
                     LLGKKVPVSLKVFLLALAIIDDIGGIIVIALFYSDFNAESVIYLLYAAFFVIVLALGG
                     KLRINGKLFYAFFGIIVWYLFLQSGIHPSIAGVVVAFTVPAHPRINIRTFIRQLRRDI
                     DLLESVSPKPTKEEIILSNTQIKYLSHIENAADQVISPLQDFEDNLYVLVNFIILPLF
                     AFANAGVVINLSDFYLSGIALAIIIGLVLGKTLGIFSFTWLTIKSGLSRMPNGMNWHS
                     LFGLSLVGGIGFTVALFLTALSYPVGSEILNQAKLGIIAGSLISGFLGYYYLKFSINR
                     GEKNNYRLKKIHRLHKIMCLNICKMPFDKKIKYSREFAKYFIKHNL"
     gene            13320..14837
                     /gene="guaA"
                     /locus_tag="PLZ54_01345"
     CDS             13320..14837
                     /gene="guaA"
                     /locus_tag="PLZ54_01345"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOJ65619.1"
                     /translation="MEKIIILDFGSQTTQLIGRRLRELNEFCEILPYNKFPEQTKDIK
                     GIILSGSPFSVYDPKALQIDLNKIRGKFPILGICYGAQYIVHSAGGKVQPGNTREYGR
                     ASLTYVDATDPLMSNIPVGSQIWMSHSDSITEVPHNFIQIGSTQDVQIAAFRIEGEKT
                     WGVQFHPEVFHTTEGLLLLENFLNICDCRRNWTPASFIETTIQQLKEQLKDEKVVLGL
                     SGGVDSSVTAVLLNRAIGKNLTRIFVDHGLLRKDEFREVLTNYEHLGLNVIGVDAKER
                     FYAVLRGIADPEKKRKIIGAEFINVFNEEAKKIENVKWLAQGTIYPDVIESYSISGNT
                     IKSHHNVGGLPEKMHLKLCEPLRLLFKDEVRSVGIELGMTPQLIKRHPFPGPGLAVRI
                     LGEVTPQKVAILQEADYIFIQELRKWDLYDKVWQAGCVLLPIQAVGVMGDERTYENVV
                     ALRAVTSTDAMTADWAKLPYDFLAKVSNEIINKVKGINRVVYDISSKPPATIEWE"
     gene            14962..15750
                     /locus_tag="PLZ54_01350"
     CDS             14962..15750
                     /locus_tag="PLZ54_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ65620.1"
                     /translation="MIIQTSMIDTHAHIYSEEFDADREKVIERALKRGIEHIILPNID
                     LQSLQPMLQLEASHPDICHAAIGLHPVSVKENFAETLNFLKFKLQHHNYIAIGEIGID
                     LYWDKTYQNEQIEAFCQQIEWALEWELPVIIHVRDSFKETMQALEPYRNKKLRGVFHS
                     FTGTIEEANQIITFGGFKIGINGILTFKNSELSKTVSQIETEYLVLETDAPYLSPVPF
                     RGKRNEPAYLSYICEQLALILGKTSEEVEFITTQNAKKLFKLQP"
     gene            15845..15934
                     /locus_tag="PLZ54_01355"
     tRNA            15845..15934
                     /locus_tag="PLZ54_01355"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15879..15881,aa:Ser,seq:gga)
     gene            16014..16823
                     /locus_tag="PLZ54_01360"
     CDS             16014..16823
                     /locus_tag="PLZ54_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOJ65621.1"
                     /translation="MKKVFAILSIVAIFSFGSALHASAQDTSAPQTEQTAGNDTAVAA
                     AQAMEQVEAPAPAQGGGMHKALKQKFIEGSPAWMAPIALCLIIGLALCIERIIYLNLA
                     STNTKKLLDDIDQAWAKGGVQAAMEVCRNTRGPVASIFYQGLSRYDEGIDVVEKAVAS
                     YGGVQLGLLETNLTWISLFIAISPSLGFLGTVIGMIEAFDRIQQVGDISATVVAGGIK
                     VALLTTVFGLIVAMVLQVFYNYILTIIENMTNDMEDSSISLLDIIVKHSKK"
     gene            16859..17332
                     /locus_tag="PLZ54_01365"
     CDS             16859..17332
                     /locus_tag="PLZ54_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65622.1"
                     /translation="MQKIPIISAIFLVSVSVILVLLIYVGGTSGSINNSAGEAMSVPR
                     FTDALIYWSYFLVGLAVVITLGFVVKNFFQSSKQSSSSIFKSLLPIIGFILLFVVSWL
                     LGSDQELKIIGYEGAENVGFWSRFTDMMIFPIYVMAIGIILTIIGTSIYSSLRKD"
     gene            17384..17986
                     /locus_tag="PLZ54_01370"
     CDS             17384..17986
                     /locus_tag="PLZ54_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOJ65623.1"
                     /translation="MSKKKRKVGQINASSMADISFLLLIFFLVTTSMNVGTGIARRLP
                     PPLPPDQKPPDMDIKKRNIFIVRINSQNQIMAQGEILDIKQLRYKTKEFIKNENNDPN
                     LPELITENIPLLGEVTYTKNHVISLQNDVGTQYQAYIDVQNELIAAYNELRDEFAMQR
                     FGKKYNELSQELQEAVQKIYPQKISEAEPKNYGGVKSGKN"
     gene            17973..18443
                     /locus_tag="PLZ54_01375"
     CDS             17973..18443
                     /locus_tag="PLZ54_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOJ65624.1"
                     /translation="MARIRKSEKREMPALSTSSLPDIIFMLLFFFMAVTTMKEVTYKV
                     RIDPPKATEIQKLENKSLIRYIYVGKPLEQYQRIYGSETRIQLSDMFADISEIEEYII
                     NERSAMKEEDQNLMSVCIRADKDTHMGIITDIKQALRRAYALKIIYAANQRTQY"
     gene            complement(18671..19714)
                     /locus_tag="PLZ54_01380"
     CDS             complement(18671..19714)
                     /locus_tag="PLZ54_01380"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOJ65625.1"
                     /translation="MKKIIVSFLMASVISSVLCQSSLTLNKNESVLIYSLPMTELCIK
                     VDVEKITRKPGVFYRYSERYLATSDIITEEKTVYSLKDIQVTSNAVPDPSKTYAVRVD
                     KNSPFVSISVDQNGLLCGINVPPPTSLPELSVLAPTETKENLSNNVILPLGEEYMLVG
                     SVAKLAEGAAKQIYRIRESRLNLLTGDVDHFPSDGTSLSAILNELDNKEKELTELFIG
                     STTKEIQKHTLYLRPQQALNNEVLFRFSVLKGLVPADDLSGVPYYISVIPTKSPTILD
                     NPKQKAPKEYINFVIPSATLVTVSDGKSTFFSQQFLFPQFGIVIPLSSSMIKGNDKIV
                     IIDPATGRLLSIF"
     gene            20160..20927
                     /locus_tag="PLZ54_01385"
     CDS             20160..20927
                     /locus_tag="PLZ54_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ65626.1"
                     /translation="MGKIISLANQKGGVGKTTTAINLAASLASLGKKVLLVDADPQAN
                     ASSGLGIEIQSLKYTIYECLIEGVSPQQAIHGTEIENLSVLPSHIDLVGAEIEMLNME
                     EREKVMQHLLRPLKPDFDFILIDCSPSLGLITVNALTASDSVIIPVQCEYFALEGISK
                     LLNTIKIIKSKLNPSLDIEGFLLTMYDNRLRLANQVYAEVKKHFQNMVFENVIYRNVR
                     LSEAPSYGKPVMLYDPESKGSSNYLDLAKELIDRQKS"
     gene            20975..21856
                     /locus_tag="PLZ54_01390"
     CDS             20975..21856
                     /locus_tag="PLZ54_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOJ65627.1"
                     /translation="MVKKSALGRGLGALIDTDDIYTSGSSSISEVDVDLIFPNPNQPR
                     SHFDEESLAELASSIRELGVISPITLRKNDDGTYLIIAGERRYRASKMLGLKSIPAYV
                     KTAADEQVVEMALIENIQREDLNAIEVALAFYRLLEEYHLTQEQLSERVGKKRATIAN
                     YLRLLRLPAEIQVGIKDKKIDMGHARAILGLEDPAVQLQLYEAILENFYSVRKVEEMV
                     RLYAETGSFEEKHVRVSKPNPIRKLKEYEELKNQLSKTFGTKVQFSCNEQGKGKISIP
                     FSNDEELSRIMGLLDKI"
     gene            21869..22546
                     /locus_tag="PLZ54_01395"
     CDS             21869..22546
                     /locus_tag="PLZ54_01395"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOJ65628.1"
                     /translation="MKRFFLITAEIWFISLFGIVLNAQNVNEKREIVQDSINRLPEKS
                     LQKESQVSIDTVQKPVYMPNPSKIVWMGAVVPGWGQILNRKYWKLPIVYGGLLGCYYA
                     YSWNASRYNSYHTAYLDIIDSDPTTNSFLDILPAGYTLESIGGTGRYTTILKSGQDQF
                     RRQRDLSVIVSVAFYALTLVDAFVDAQLYGFNISPDLSLEIKPIYLKDNITTSGGLGM
                     QCRFKLQ"
     gene            22572..23849
                     /locus_tag="PLZ54_01400"
     CDS             22572..23849
                     /locus_tag="PLZ54_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOJ65629.1"
                     /translation="MNLKFSIIVVSFICFSINSVISQAAKDFPEDTLSSKIDSIIGIP
                     DAYGYALDHLLHLWALQRMDTTECNSGEPPAPVSDSLYKYRLSQIPCLIELPYNNIVR
                     SFIELYTVRKRTQTEYMLGISNYYFPVFEQALDANKLPLELKFLPIIESALHPTAISR
                     AGAAGLWEFMIPTGRMYGLEINTLVDERLDPRKASYAAARFLKDLYAIYGDWNLVIAA
                     YNCGPGNVNKAIRRAGGKTDYWAIYPYLPLETRGYVPIYIAATYTFHYAEEHNLCPAV
                     VNIPALIDTFMINQRIHLEQISSVLNIPIEELRWLNPQYKKDIIPGDLKPYVLTLPVN
                     YSSIFMERLHEIASYKADELINNRRNEIEAFRSTSSSNLGASKNVTYHKVKKGETLSG
                     IAAKYGVSVSNLKKWNNIKGSKITIGQRLKIIK"
     gene            23858..24178
                     /locus_tag="PLZ54_01405"
     CDS             23858..24178
                     /locus_tag="PLZ54_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOJ65630.1"
                     /translation="MEKVYYSISEVAEMLKISPSNLRFWEKEFSQLKPKRSNKGTRTY
                     TKEDIEIIKMIHLLVNEQKLTLQGAKRKLSLKKETVSKQQELVERLQNVRRELLMISK
                     ALDE"
     gene            24202..25272
                     /locus_tag="PLZ54_01410"
     CDS             24202..25272
                     /locus_tag="PLZ54_01410"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOJ65631.1"
                     /translation="MKKTNFCLGLIAALILMSASVSAEKKPKNVILMIGDGMGLAQMY
                     SAMTANNNQLQLERCTYTGFSKTYSSDHYTTDSGAGGTAIACGVKTKNGMIGMTPDSV
                     KTESILELSERNQLSTGIVVTCAVTHATPASFIAHQISRNMYEEIAGDFLQTDVDVFI
                     GGGLKYFNNRKDQINLVEKLKKNNYQIAYSLDEAVKVKSGKLAGLFYENHCPPMPERG
                     DMLPKSVMAAIDILDNNKKGFFLMVEGSQIDWAAHDNDKKQIVKEMLDFDETIGKVLD
                     YAQKDGNTLVIITADHETGGMTILNGKFGYDEMDAHFSTKNHSGVPVPIYAFGPGAEQ
                     FTGIMENTAFKGKIEKLLDLKK"
     gene            25369..26476
                     /gene="prfB"
                     /locus_tag="PLZ54_01415"
     CDS             join(25369..25431,25433..26476)
                     /gene="prfB"
                     /locus_tag="PLZ54_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445733.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOJ65632.1"
                     /translation="MITIEQLKDVTERTEALRRYLDVDNRQIQIEEEELRTQEPNFWD
                     DPKKAEAQMRKLKELKNWVTGYQEVKTAADELELAYDFFREGVIEEQELDETYRKALQ
                     LIEQLEMNNMLSHEEDQLGAVLKIVAGAGGTEAQDWAEMLMRMYQRWAERKGYKWEIT
                     NFQPGDEAGIKSVTIQIEGERAYGYLKSENGVHRLVRVSPFNAQGKRMTSFTSVFVVP
                     LIDDSIEIEINPANLSWDTFRSSGAGGQNVNKVETGVRLHYTFKHPVTGEQSEIVIEN
                     TESRSQFDNKDNALRLLKSQLYELELQKRRAEQEKVESTKKKIEWGSQIRSYVFDDRR
                     VKDHRTNYQTSNVQAVMDGDIDEFIKAYLMEFPD"
     gene            26909..28570
                     /locus_tag="PLZ54_01420"
     CDS             26909..28570
                     /locus_tag="PLZ54_01420"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOJ65633.1"
                     /translation="MKETKYIFVTGGVVSSLGKGIIAASLGKLLQSRGFKVTNQKLDP
                     YINIDPGTLNPYEHGECYVTVDGHEADLDLGHYERFLNIETHRANNITTGRIYQNVIE
                     KERRGDYLGKTVQIIPHITDEIKRNIKLLGSKGDYDFVITEIGGTVGDIESLPYIESV
                     RQLRWELGKNCLIVHLTYVPYISAAKELKTKPTQHSVKALQEQGVQPDILVLRTEHNI
                     SSDLRKKVALFCNVEPNSVMQSVDVSTIYRVPLMMYDEKLDEVVLRKMGFDLLKIPQA
                     NLSKWIQFVEKLENAKEEVHIALVGKYVQLPDAYKSIIESLLQACAYNDRKLKLDLIL
                     SDKLTEENIEKKLEGVDGVIIAPGFGQRGIEGKLVTIKYTREKNIPTFGICMGMQCMV
                     IEFARNVLGYADANSTEIDPTTTHNVVDIMEEQKNILNLGGSMRLGAFPCKLKKGTKV
                     FKIYGKENISERHRHRFEFNNYYKEEFDKAGMICSGINASSNLVEIVEVPSHRWFIGV
                     QFHPEYSSTVINPHPLFISFIKAAIEYRNESKEKTQNSKFKTAIK"
     gene            28627..30531
                     /gene="yidC"
                     /locus_tag="PLZ54_01425"
     CDS             28627..30531
                     /gene="yidC"
                     /locus_tag="PLZ54_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOJ65634.1"
                     /translation="MDKNTITGFLLITVIIVVFALLNRPNQEMIEKQRRYNDSIAQIE
                     KAKAESETAIQEVNSLLSDSIKTTLDSSAVIRDAYGDFSVAALGKEEFITLENSVLKL
                     TFTNKGARIYAAELKDYRRHDSLPLILFQGSEANMGFTLITNNNRILNTANLFFEVLL
                     PIEKDTLGNQILVFRLKTTGEGYMDVVYTLPPDNYMFSFSIKANKMSNVMPAGTNFLE
                     MQWTTRLRQQEKGRQFESRYSNIQYKYLSDDVEKLSESKNDSKQITNKLKWIAFKDQF
                     FSNVLIAEDAFTSSFLESKVEPEDSPYLKSYRADMTIPFDPTGKTSTNFRMYLGPNKF
                     NILASYDKGVAKEEKLQLHKLIPLGWGIFGWINRYMIIPMFNFFSKYIGNYGIIILLM
                     TIIIKIIIFPMTYKSYTSSAKMRVLKPEIDEIYNKIPPEKAMERQKAVMDLYSKVGVS
                     PMSGCLPALLQIPILFAMFSFFPASIELRQESFLWAKDLSTYDSIISWNAYIPIISKY
                     FGNHLSLFCLLMTISSIISTKLTMTTTPTTNQPGGNLMKWMMYLMPVMFMFIFNNYSA
                     ALSYYYLLSTLITIIQTFIIRSMVDEKKLLAQLHAKRNQKQPAKKKSSWIERMEKMQR
                     EQQKALKNKR"
     gene            30613..31623
                     /locus_tag="PLZ54_01430"
     CDS             30613..31623
                     /locus_tag="PLZ54_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65635.1"
                     /translation="MTKKIKFFILFFLIIFLFSCQNHNRFRINTKKNEVEVRIRRFDK
                     DLINVDTTNLKAEIRKIYKDYPVFFPVFINEILNVAPSDTDSVAKLIGDFLCDAAFQK
                     VNKEVLKTFADVHDIEKQLSIAFTYMHHYFPEIRLPEIYFFVSGFNRSIIIYPSLIAV
                     GADLYLGADYPLYKSFTYEYLTFNMRPESIAPDVVSTLLFSYFPMESENNRLLDNMLY
                     NGKILFLLSVFMPDVEPSVLMGYTSEQWQWCKRFEKEIWAKIIEDKALFSSDMRLINS
                     YLDEAPFTAPVSQDSPGRVGTWIGWQIVNSYMKKNNNVSLRQLANENNYQLLLEKSDY
                     RP"
     gene            complement(31690..31857)
                     /locus_tag="PLZ54_01435"
     CDS             complement(31690..31857)
                     /locus_tag="PLZ54_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010663521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ65636.1"
                     /translation="MAYVITEDCIACGSCITECPVDAISEGDIYVIDPDVCTDCGVCA
                     DVCPSEAIIPA"
     gene            complement(32234..33841)
                     /locus_tag="PLZ54_01440"
     CDS             complement(32234..33841)
                     /locus_tag="PLZ54_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ65637.1"
                     /translation="MDRNKKNYLAPLIVFLAVIFGLILGNLLAQRAFFSSSPNIFFGF
                     GNASKVDELLQIINTNYVDTLNLDEMTENVMQDIIAKLDPHSVYIPAEDISLANSELE
                     GSFSGIGVQFNIQNDTVMIISVISGGPSEAAGLMPGDRIVEVNDTTFVGKSINNEKVM
                     KKLRGPEGTKVKLGIKRNQTKEVLYYEITRGKIPVNSVDIAYIVQPSVGFIKVSKFAE
                     TTYGEFLNAIAKLKNEGADKFIIDLRGNSGGFMDQSINMINEFLPANRLIVYAQGKAY
                     KRFEAKSDGKGSCTREPLVVLVDEFSASASEIFAGAIQDNDRGTLVGRRTFGKGLVQQ
                     QFNLSDGSAVRLTVARYYTPSGRSIQKPYKLGNTEEYEKEFINRLLHEINDSIFHPDS
                     LRYKTVGGRIVYGGGGIMPDVYVPLDTSEFTPYFNNVVNYGYVYQFAFQYSDKNREKL
                     KKIKNWHEMDTYLNSQSLLDQFVKFVSEKGIQANKKEINISKNLLLTQIKGYITRNIL
                     GDEGFYPLLYRSDKTVLKALDVINSKQ"
     gene            34300..34677
                     /gene="gcvH"
                     /locus_tag="PLZ54_01445"
     CDS             34300..34677
                     /gene="gcvH"
                     /locus_tag="PLZ54_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOJ65638.1"
                     /translation="MKVPENLKYTTEHEWIRVQGNTAWVGITDFAQHELGEIVFVEVE
                     TLGENIHAGEVFGTVEAVKTVSELFLPISGEILEFNKELENKPELLNDDPYGEGWIVK
                     IRVTDPSELEKLLSAEQYAKLIA"
     gene            34709..35215
                     /gene="purE"
                     /locus_tag="PLZ54_01450"
     CDS             34709..35215
                     /gene="purE"
                     /locus_tag="PLZ54_01450"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOJ65639.1"
                     /translation="MKARASIIMGSTSDLKIMEKAAKLLDEFEIPFEIHALSAHRTPK
                     EVEDFALHAEKRGIEIIIAGAGMAAHLPGVIASMTTLPVIGVPIEASLNGMDALFSIV
                     QMPPGIPVATVGINAAQNAAILAVQILALKDEKINSKLKLYKENMKQKIVEANNQLKE
                     IKYTYKTN"
     gene            complement(35434..35619)
                     /locus_tag="PLZ54_01455"
     CDS             complement(35434..35619)
                     /locus_tag="PLZ54_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65640.1"
                     /translation="MMKAFSHRTKVRNLGNITTKLETKRYSVGYLEKGEIGEKTKNKM
                     SFSSGDGVIKWFSLPSG"
     assembly_gap    35653..35752
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            35844..38063
                     /locus_tag="PLZ54_01460"
     CDS             35844..38063
                     /locus_tag="PLZ54_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOJ65641.1"
                     /translation="MTTYPSQSVFSLYRRMVTSEKNMFSFQERKAIKQILKEYYLLHP
                     QQLTLPGHSIEDKLYIIQILLNEIGLGKASVLSVLFMEPIKNNYLTVSEIEQKFKTPL
                     DSIIGGMQRVEELYERSFSLETENFRKLLLTFAQDVRVILMMIAERLYFMRILDKFPA
                     DMQQSMARECSFLYAPLAHRMGLYSIKSEMEDLSLKYTNREIYREIAQKLNETKRSRD
                     KYIEEFITPLKEKLGKEIKYPFEIKGRTKSIYSIYSKMLKQNTPFENIYDLFAIRIIL
                     DVPLEKEKMICWQVYSIVTDMYQPNPKRLRDWLSIPKSNGYESLHITVLGPQNKWVEI
                     QIRTTRMDEIAEKGLAAHWKYKGIKGESGMDEWLKNIRDILENPDLNAVDFMDEFKLN
                     LYDQEVFVFTPAGDLHKLPKGATVLDFAYLIHSNLGSKCIGAKINKERNVPIKYVLKN
                     GDQVEILTSPSQKPNPSWLNIVVTSKAKSKIRQALKDIEFKDAEIGREILLRRFKNWK
                     IELEEGKLSRLAKKKGYKTVSDFYQAIAHEKIDMLLIRDAYLDLDRHETGAEQAEPRS
                     AETFSKETPAEELSSKEDVLIIGQNLKNVDYTLAKCCQPIYGDDIFGFVSVGGGIKIH
                     RTTCPNAPQLISKYGYRIIKAQWAGRSGGTQYPITLYIVGKDDIGIVTNITSLISKEK
                     NILLRSISVDSNAGVFQGNLTIMVNDANDLDSIIKKIKQVKGVLSISRTKSLTQNNQ"
     gene            38077..38676
                     /locus_tag="PLZ54_01465"
     CDS             38077..38676
                     /locus_tag="PLZ54_01465"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOJ65642.1"
                     /translation="MFSGIIEEAAPVVALKKEAGNLHITMTCSFVNELSVDQSVSHNG
                     VCLTVVSKTPDTYTVTAIRETLEKSNLGLLQEGSKVNLERSLLLTERLDGHIVQGHVD
                     TTAVCTDISEADGSWYFTFQYDVDRELAKRGYVTVDKGSVAVNGVSLTVCNPTENTFQ
                     VAIIPYTYEHTNFHQICVGTTVNIEFDIIGKYISRLIKL"
     gene            38761..39918
                     /locus_tag="PLZ54_01470"
     CDS             38761..39918
                     /locus_tag="PLZ54_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ65643.1"
                     /translation="MENFRKQIKISFFNPIIHFLPLLLFLVTAMYAGRTVANYVSIFV
                     LLVSAVYVFIAYNQIFIWFFLSCVIFLAVNIVGSIIRLPIIFLSFRPLLDEALTLVFL
                     GGMMLLQKRIKKGIMALIPKTLPMNNNLDEYFGFIRTIFLIILGYVLATGFFYAFPTQ
                     RETAYLQLVQAIYVGILFFVLVFESLRVQIIRARLLKEEWLPIVNSQGKIIGSVQHQA
                     SLGEKRKFMHPIIRVHIIQDGMILLRKRPADDLSYPLHWDTAISNHIRMGETVEDCIK
                     RTAQELYGLTELKSFFLSNYITHTDFEQHYAFLFIACKMNKIDLQSEKVKQAKWWTLQ
                     QIEENLQEGIFTENFLIEFDLLKKSGVLENNICDCACKLKETIFKAPHLFR"
     gene            39940..40515
                     /locus_tag="PLZ54_01475"
     CDS             39940..40515
                     /locus_tag="PLZ54_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65644.1"
                     /translation="MMRLYFILLLIAFVSCGGHSNSEFKLQNSFQIKLQDSICINAKD
                     CFYFSTDSNLYRIFMYFSNAELKEKKIIDTVDFSPYKSKIHSFQSENNESYVVLWETE
                     YEFYPLIYVYFITAGKIVKIGELLISLPCQSCESFEYPIKDIRMLQKGMNIEISFLKD
                     VNYKPSNGNEWHLYKAGMLKCIFNAETNKLK"
BASE COUNT        13277 a         7145 c         7647 g        12607 t
ORIGIN      
        1 cgatatggac tccttgctga cttgcagtta aaagtccttt tttaatttct tcaaataatg
       61 aataaatgtt ccaataatat ttgtcatgaa tacaaataat tttattctga aaacgagtaa
      121 gttcctttaa atcaagtcgg ttattttgca aagtacctaa aatggttcgc ccactggtgg
      181 cacccagatc tacagcaaaa aaatgaggga gtttactcat agaatttatc ttgtattatg
      241 aatgcagaaa atttattaat gaatctacct aatgtaaatt tcaacaaaag tacattaata
      301 accataagaa aatactaagt attttgatat aaatactttg aaatttggca taaaatgtaa
      361 attttttttc ttgtaatctg tggaataaaa atttttccct tcacaagttc aaaaaaaaaa
      421 ttaattttgt catgaaattt caaatcttta aggatcggag aaaagaaata gacgtttaat
      481 tttcagatat ttacttgttt cctttgtctc taattttaca agcagattaa tacatgaaaa
      541 acatagaagc aaaaaactca attctttgcc gctatctcat cgcaaacgag caagatacaa
      601 gatggggatt gacaataact acggttggta aacaaagctt aagcatcaat gaagattatc
      661 cgccaaaaga tggacatcct atgggatatt tattttccac cgaacaaggt cgtgtattgg
      721 atgaatatca attattatat attgttaaag gttcaggaat tttcacatca acttcttgca
      781 agcttaccaa tataaatgca ggaaatatgt tcatgctttt ccctggcgaa tggcacaatt
      841 ataaatcaaa tccagcaaca ggatgggaag aatactggat tggatttaaa ggagaaaata
      901 tagataaacg tgtaggaaat ggcttttttt gtaaagaaaa tcccgtttat aaggtaggag
      961 tattggacga agctgttcag ttgtataatc aaggaatttt agtggcaata gaacagaaaa
     1021 gtggttttca acaaatgttg gcaggaatag taaatcatct gttaggatta gctttttgtt
     1081 taaataaata taatgaattt gaaaatctgg aaattacaaa tcttataaac gaagctaaaa
     1141 tcagatttat ggaagacttc cggaaagata tagatattaa agatgttgcg aaaggattaa
     1201 atatgagtta ttcaactttt cgtaaggttt ttaaagaata taccggtttt gctccggcac
     1261 aatttcttca agagatacgt atacaacatg caaaagaatt attgacaaac acaaacttga
     1321 atattaagga aatagcattt gagagcggat ttgagaatcc ggaatatttt tttactgttt
     1381 ttaaaaacaa aaccggatat actcccatcc aatatcgaaa ttttactcaa gagaaaatta
     1441 gcatctaaaa attgtcattc tgttttttgc tattttgttt attccgaaaa ttatgtgctt
     1501 ctttttaatg atttgaatga cagattaaca acatattatg aatgtaattt ccattaaact
     1561 ttttctttac aggtaagagc aaacgtattt cataccatct ctgattttat tagcagttgc
     1621 ccattaattt atcttattag aataaataat ccttaggcaa gcgttttgaa attcttttga
     1681 ttaaaaaaaa gaggaaattg ttcaattaac tgtttctgca taatttcatt caattgtggc
     1741 aggtaagcat ccggctcacg tcgaaaaggg tagtgagatc tcagatactc aaacagtcga
     1801 ggatgatttt ttaactgatt gctttctgat tcgatattgt aagttttcag aaaaaaatct
     1861 tctatgttat taacaaacga caaatcgact ttagtaggtt cagggagatt agagacttcc
     1921 caattatcaa gtcccagacc aaaaaatttg cttaccgacc gcacactttg agaagcagcg
     1981 ttggctttgc cttcggccga atatccggca atgtgtggag tacccaatgc agttttattc
     2041 aacaattcgg tatcaatatc aggctcattt tcccaacaat caataacagc agtcaatatt
     2101 ccttgtctgt tcaaagcatc caacaatgca cttgtagaaa ttacttctcc tcgagcagca
     2161 ttaataatca ccggtttttt ttttaatgta tcgaaaaatt ttttatcggc taaatgaaaa
     2221 gttttatctt caccttccat attgaggggt gtgtgaaacg agataatatc ggacgtcgaa
     2281 caaatttccg ataaagaaac gaattggtaa gggtcttctt ttctggcgcg aggaggatcg
     2341 ttttgcaaca cattcatacc aaaagaagct cccaattgaa caatttttga acctacagct
     2401 cctaccccga ccactcctaa agtttttcct gccagctcaa aaaaatttct atttacataa
     2461 ctgaacaccg aagccatata ctgagcaacc gaaagtgcat tgcaaccggg cgcattggtc
     2521 cactttatac cgttttcggc acaatatgca gcatctatat gatcgtaacc aatagtggct
     2581 gaggcaataa acttaacttt agaacccgac aacaaacgtg catcgcattt ggtacgcgta
     2641 cggactataa gggcatctgc attttttacg gtttcattcg taatttgaga atggggcaga
     2701 tatgtaacat ccaacacctc atccaataca ccccgaaggt aaggaatata actgtcaatg
     2761 acaatacttg gcacttttga ttgaaataat tgaattgcaa aattacatga aaattgaatt
     2821 atttccaagc ttgtgaaaat aaaaatcaaa gttgtaaagc caacaggcaa gtgagaaatc
     2881 aaataaaatg gtttaaaaaa atatttttcg acaataattt tactatcctg ggcataaaaa
     2941 cagactttat aaacattgat taaacgataa agcgccttaa aaatcaataa attctcagac
     3001 gctttattat tagtaatcag ttcaagaaaa attttttgaa ataacccttg aacccttttt
     3061 tgcctaaact tttctaatta ctttggttgc caataacatc ttttgggtga ttctatggct
     3121 ccttcagctt tcagctcgtt catggctttg tctacttcct ttctttcgag accggtcatt
     3181 tcgacaattt ttcctgcatt caccggcgaa ccggccttct tcatggcttc cagtacaatt
     3241 tctttagtat tcatagctgt taattttttt gtttacaaca atcatttccc gaaaatgttt
     3301 tcaatacaaa gcttattcga tattttttct gactttttcc aaaaattcct gcattcgtgc
     3361 cgcgtccttc tttttaatga tgtccagcaa cactttcagt tgagctcgaa tgttgtcgat
     3421 ttgttcttcc gcatagggat tgaaaagaat ttcagtcaac agataatcgt cttccgataa
     3481 aagcccttct gcaatgtcca tgtgcctttt gaaagtggtg ccaggtgcat cctgatgttt
     3541 catgacagct gcaaaaacca atgtggatgc aaatggtata gccagcgaat atgctgttgt
     3601 tttatcgtgt tcttcaaaag agtattcaaa gatttttaaa tgtagagaat ggtacagatc
     3661 tttgaaaaac atttttccaa gataatcgga ctccgaaata atgatggcat tctgagaact
     3721 taaattgtcg agcgttgcaa atgttggtcc aaacatgggg tgagtggaaa caaaacgttt
     3781 tccggtactt tcgtaaaatt ccttcaaacc cgttttaacc gatgcaatat cggacaacat
     3841 gcagttttcg ggcaaatagg gcaacacatt tttaaatgcc tctatggtat acttaagcgt
     3901 aacacaattg attaccaatt caggttcaaa ctcttgtatt tctttgtatt gagtcagccg
     3961 ataagtatta aaaacaaaac gcaacttttg tgggtcaacg tcataaagag ccacttcgtg
     4021 atggaggcac aataaatcgg tgaaaaaagt gcccatcttc cctgctccta aaatcaatat
     4081 tttcatcaga atataattta ttttttaaag gtctgatgga acctacatgt ctgaatatta
     4141 tattcattcg tgtttgtgta tgggatacat gtgggtttca taaattgtta tttttcaatt
     4201 tcgaatacaa aagtggcaaa aatgaaaaca aaacgattca ttttacaaat aagtagaaag
     4261 atttcttaca cagatttctt tttcccgaat tccttgtcta tcttcataaa cggaataatg
     4321 gcaagagtcg gaatggcaca aatcagcacg aaaataaaga aatgattgta ccctatcaat
     4381 tcttgcagcc aaccggccat catcccgggc aacatcattc ccagcgccat gaatcccgta
     4441 caaatggcat aatgggcagt tttgtgttct ccttccgaaa aataaatcat atacagcata
     4501 taagccgtga atccgaatcc ataaccaaac tgttcgaaag cgactgccgc attaataagc
     4561 aacatattct cgggcatgaa ccacgacaaa tacaaataag ccaagtttgg cagcgtgatg
     4621 cacaaggcca tcggccaaat ccaatatttc aatcctttgc gcgaagctgc aatcccgccc
     4681 acaattcctc ccaaagtcaa ggcaatgacg ccaaccgttc cataaaccag cccaacttgt
     4741 cctgtagtta gtcccaaacc tccaacttca tgcggatcga gtaaaaaagg agaagacatt
     4801 ttcaccagca ttgcttcacc caaacgataa agaagcatga atgcaatagc caacccgatt
     4861 cctttctttt ggaaaaatgt ggcaaaagtt cgtccaaatt ctctaataat atcttttgac
     4921 gaatgagtca aagatgggtg atcggaagcc ggtcggggta aaataaacct gtgatacaga
     4981 taaaataaga taaataaacc ggacaaaatg aagaaagtta tgctccaagc taatctgata
     5041 tttcctgaaa ctcccttaaa ttcgcttacc gcctgtccct tcagcttagc atcgagctga
     5101 acaactacca gcgcgggttt atcccaattt tcatcggtaa aaaccaaacg ttcgccactg
     5161 acaagagaaa tacttttatc gcctttggac tgacttaaat tcaaaatcaa cggtttaccg
     5221 gcttctggtt tttttgtcaa acgtatggct acaacaccgg catttcccgt taaattctct
     5281 accattgaaa tcttttcccg tttttctcca aaattttccc tgataaaatc ggcaaaaggc
     5341 cctgaaacat gttgatgcca ccaagaatcg ttttcttttt tggtctgatt attttgaagg
     5401 ttttcttccg caataaattg gtagcgtttg ttgttctcca gtgcagcggc tttgatttta
     5461 ttgagcgaat cgctggtaat aggttgcgta ctcaaactca agctggcagg gaaaactaca
     5521 aatttcatct cggcatcttc gggaaaattt acttttgaca gactgtctac atttactatt
     5581 tgagaaattg gatccggaga agattgaacg gaaaaaacaa ccggttccat gccggtatgg
     5641 ctttccaaag ctcccgcaat aatgatgaga attccctgac ctgcaatcat ggcaaaacga
     5701 taaaaggtac tgcgaattcc cacaaaaaaa gactgttgtg agctatcgag cgccagcata
     5761 tagaatccat cggccgcaat gtcgtgagtt gcagaactga aagcaatcaa ccacagtaca
     5821 gccaaagtag cctgaaaaaa gaaaggagcg ggaattaaaa aagctactcc agcaaaaccg
     5881 gcaccaatca gcaactgcat ggaagtaatc caccaacgct tggttttaat taaatctaca
     5941 aatggactcc aaaatggctt tattacccaa ggcaaataca accagctggt atacaaggca
     6001 atatcggcat ttgaaatacc cagccgcttg tacatgatca cagctactgt catggcaact
     6061 acataaggaa tgccttcagc aaagtataaa gtgggtaccc atgcccaggg agaaatgttt
     6121 tttgtttgct tgattttcat gtcgatatat cggaaaaatt tagaggttat aaattctgtt
     6181 tctgatcatt tcaaacatat aaacttttca tagcaacttt aattaaagtc agggtgaggg
     6241 ttataaataa attttggaaa cgagtttaaa atatttctaa ctcaataaaa ttgtaacaca
     6301 atatatggga acatcccttc tttgcttaag aattagaaaa atacaaacca tgacagaaaa
     6361 aatcgaactg aaaaacaatt gctttcaaat taagaaaggc aaaggtaaac aatcttctgt
     6421 gaatttaaaa gaaaaaatga taaatcatct accaacaatt cttacattta tatgctgaaa
     6481 ttcccttcaa tttttcaagg gaaatatttt ctcttttttg gaaattaatt taaataggta
     6541 taattttgaa acaaaaaata tcgtaaacag acagagtaat gagaaaaata tttttttttc
     6601 ttagcttatt ttttttcccc atcctgattc aagcgcaaac tttttctgga agtgctttaa
     6661 ttacaggtaa actgattgat aaagaaacaa aaaaacctgt accattagca aatatccggg
     6721 ttttacagca gcgcgacagc agctttgtaa acggacaggc gagcaggaat gatggatctt
     6781 tttccattcc ggttcattat ggatcttaca ttgtacatat ctcattttta ggttatgatg
     6841 atgtttttaa gcacgttcaa gtatcagctt caaatcctat tgctgtgctt gatactatat
     6901 ttttaagaca gaccgatttt aagctttctg aagttataat taccgagaaa gcgcctgaag
     6961 ttagagtgaa aggagacacg ctggaattta atgcaggttc gtttaaagtt actgaaagtg
     7021 ctgtggtaga agacttactt aaaaaaatgc ccggtgtgga aattgataaa gacgggaaaa
     7081 ttactgtcaa cggacgcgaa ataaaaaaaa ttatggtgga cggaaaagag tttttcagca
     7141 gcgatcccaa agttgcgtcc aaaaatctac ctgccaaaat ggtagaaaaa gttcaagtgc
     7201 tggataaaaa atccgaaatg gctgaaatga ccggctttga cgacagcgat gaggaaactg
     7261 tgataaatct ggttattaag caagggatga aagaaggatt gataggaaat gcctttgccg
     7321 gatatggaag taaagaccgc tacgaaggca atgctatggt caattacatg agaaaccaaa
     7381 atcagtttac cttcataggc ggcctaaaca ataccaataa tgcaggattt accgatttgg
     7441 catcaagtat gtttggtaac atgggcggtc gcggtatgcg aggaatgttt ggaaaccaag
     7501 atggagttac tacttcgggc atgggaggat tcaatttcaa cacccaactt tctcaaactt
     7561 tcaaattggg cggaaatgtt cgttatggtt ccacaaacag tgatgtgttg tcaaaaatcc
     7621 ggacacaaaa catgctgagc agcggaaata cttttgaaaa cgaaaatgac accacgaaca
     7681 gtttcagtca aaattttaat atggatttac gaatggaatg gactcctgac acagttaccc
     7741 gcatcatatt ccgcccaagc gtcggggtgt atgaaaacag tcgcgatgaa attagtaatt
     7801 tcaatacaac caatgaagaa aatgattcta taaataaagg tgattcaaag tattcttcgg
     7861 atggaaacgg atacaatgtc ggcgggcgct tggaagtaag caggcaattg gggaaaaaag
     7921 gacgtgttct cagcctccaa ctgagaggac agttgaatga tgccgacaac gagggttaca
     7981 atctttccaa tacttattat tttggtacca aacctgatga tattattgat cagaaattta
     8041 ccaataccag caacagcaaa aactggcaaa gttttctatc ctacgtagaa cccatcggac
     8101 acaataactt tattcagctt gcctacaatt atcagcaaaa attttccgaa tcgaacaagg
     8161 atacacgaac caaagacgac ttgggaaatt attcggtaat ggatactacc tacagcaaaa
     8221 gattggaaaa cgacttcgtt aaccaatcga tagaaatgaa tttcaaagca aacagaggaa
     8281 aatatgacta tatgtttggc ttcagtatgc agccgttcaa ttccagcagt aaaacctata
     8341 aaggagattc tctttataac aatacttcca atcagggtat caattatgca ccaagtgccc
     8401 aattcaatta tcgatggagt cgacaaaaca atttaagaat caggtattcg ggcattacaa
     8461 gccaaccttc cgtaacgcaa ctttcaccgg tgagagatgt ttccaaccct ttaaacgtta
     8521 cttatggtaa tcccgattta aaaccttctt tctcccatca attgaatatt cgctatatga
     8581 attttatgcc gcaacaaaaa agttcaatca atttttttgg aaacgcttcc tacacgttga
     8641 acgatattgt ttcttcgact tctacagacg ttttcacggg acaaaaagaa accacttata
     8701 aaaatgttaa tggcaactgg aatgccaacg gacgactgac actgaatttg ccaataaaaa
     8761 aaacgaattt ttccgttttt tccatgtcat caggcagtta tactcattcc aaaggatatt
     8821 ccaataatga aattaactta agtcagcgaa ccggtttggg acaaacactg ggaattgatt
     8881 ttcgttcggg tgtcgtggat ttcggcacaa ggggaaacat caattacaac aatattcaaa
     8941 acagtttgac aggtcagcag gatcaggaat atttcaatta caatgtttca gccaatacca
     9001 ctatttattt accttatgac ttatcgatcg acagcgacct atcttacaat accaattccg
     9061 gctattcaac gggattcgag caggaagcac tgatatggaa tgcttccctt caaaagcagc
     9121 tttttaaaca caaaaatggc tccctgcgct taaaaattta cgacattctg caacagaaaa
     9181 gcaacatcag cagaagagca acggccaatt atattacaga cactactacc aacacgctaa
     9241 cttcctattt tattgttcat tttgtgtatc gtttcaatat tttcaaaaac ggcgcaaatc
     9301 aaaacgacat gatgccgcac agaagctttg gtggaggacc cagtggacac tgaccctatg
     9361 ctgacaatac ttgaaaaaac agaacaataa ctactgtatt aaaatcataa atgcaactgt
     9421 ttataggaca aattttgtaa taaaaattca gttgttataa cagattaagg tttttatatt
     9481 ctttttcttt gctttttcca aaagatttac aagagcaatt ccgaacaaaa tataaccctt
     9541 gaacatgatg tattgcatga ataaaaatta tttttttgta acaaacacat aaacaatgat
     9601 ttatactatt atttttcaat ataaaaatct gaaaaaatta aaatttttta tatactgaaa
     9661 taatttttct attttttttt gttatttgaa aaaaaagcat ttcctttgta tccgattaca
     9721 agaagtgtat ctccctcgat cggttatttt caactttcca tttctctgga cggcaatctt
     9781 taacctcgta tgtttgtttt actcctcttt aatcgcaaat tatattaatt tctaattttt
     9841 aattttttta tttttatgaa catttatgtt ggtaacctga gctacaaagt tcgggaaaat
     9901 gatcttcaag gggttatgga agaatatggt caggtatcat cctgcaaaat cattaaagac
     9961 cgtgaaaccg gaaggtcaaa aggattcgga tttgttgaaa tgagtgatga cactgcagca
    10021 acaaaagcaa ttgaagaatt gaatggagcc gaattcgatg gaagaacaat ggtagttaaa
    10081 gaagcacgtc caaaaaatta agcttttttt aaaaatcgaa atggagaact tctattgtcg
    10141 caagacaatg gaagtttttt gtttttaact gttttcacga atgattaatg atatatcgac
    10201 agaagtatga gctcattaaa aggtgttttc aaaaatcgta aaattttttc tcaattattg
    10261 ctgttgttgt ttttaagtgg cttgtttttt tacattggtt tagctttttg gatggttata
    10321 tcggcaggaa aaagagaaga cgcccaagcc atccgagtat tacaactgat acagtcaatc
    10381 ggattgtttt tgattccacc atttattttt gcatatttag tgagcgatcg taaaccgaca
    10441 gaatatctca gtttatctca aaaaatcaac ttaaaggatg tgatgtatgt gatactaata
    10501 atgtttattg ctattccatt tgtcaatttt cttacctctc ttaatcagca actggtttta
    10561 ccggaggtac ttgcacctgt ggaagaatgg atgaaacaaa cagaagaaga agctgcagtg
    10621 cttaccaaac aacttttaca ggtcgacaac ataggtggat tgtttgcaaa tctaatatta
    10681 attgccttaa ttccagcatt gggagaagaa ttgtattttc gtggtgcttt acaaaacgtc
    10741 ataggtcgtg gcaaaggtac ccactttgcc atctggatga ccggtttttt gtttagcgca
    10801 tttcatttac agttttatgg ccttttgcct cgattgttgc tgggagtgtt ttttggatat
    10861 atcttggtgt ggagtggaaa tatatggctg ccagttattg cgcattttac caacaatgca
    10921 atagcagtga tattttacta cttcagcgga caaaacaatg aagtagtaaa cattgatgct
    10981 gttgggacaa atgatagctg gtggatgggt gtaataagtt tgattttaac cattttgctt
    11041 gttttcagat taaaatcaag aattccaaaa aagcaacata tttaacaggc atttcttctt
    11101 tcgcctacta tcattaaagc ttcctctctt aaaaataaag gtaattctat ctgcctcatt
    11161 tgtaaactgc gtaaccaacc tgccacatcg ctttcttgca aactgtagaa aacttcagaa
    11221 agctgttcta tttctttatc ggaaaattgc ttcggcgatt ttccactcaa agcatcaagt
    11281 tcttcgtcat cataatattc tatttcattg tccatgttta agagattttc tctttcacaa
    11341 agagcatgtg ctccacagca ttctgaattt tctcgaactt gtaccgttgc ttcttctctt
    11401 ttgccttttc ggctaaaata ggtaactgtc attaaaacaa gtccgactga aatcaaaatt
    11461 attatcaata cccacataat tgtttgtatc ctttcttagt tgtttataaa tatacaacaa
    11521 attttttgac tgtaaagtta gtaataaaac tgaaaaaatg tttgttaaat tgtgtatgaa
    11581 ttttcggtcc cacttatgtt ttatttctcg tacactaagt tatgcaaaaa atattttttt
    11641 gttttctctt taatattttc ttacctttgg acaattttta cagaaagatg tccctctcaa
    11701 gggtatagat tttgatatct ggtattataa atcttttttt tgtagaattt ggatgcatat
    11761 ttaccattaa tctaaggatt ttactcatag atgcttaatt cctgaataaa tatgattgat
    11821 caccattcga aaagagcaag aatatatatt tttttaagaa attttaaatg gaaaactaat
    11881 ccgggtattg tcatgatggt agttgccatc gcaaccctta ttattgccaa ttctccattg
    11941 aaggaatggt atcagtcatt ttggaatata gaatttttac ttggtacaaa ttcattcaac
    12001 ctttttagtc atcacggtac accccttacc ctgttgagtt ttatcaacga ctgcttaatg
    12061 gcggttttct ttttttctat aggtttggaa ataaaacgag aagttctgat tggagaactt
    12121 tcatctttca ggcagtcgat tttacctatg attgcagctt tgggaggaat gattgttccc
    12181 gttttgtgct tttttgttac gggtcgaata cagaaccttt cgccagatga aatgagagga
    12241 atgtttatcc cgatgtcaac cgatatagct ttttcgttgg cagtggtatc gttgttgggg
    12301 aaaaaagttc ctgtgagttt aaaggttttt ttgcttgctc tggcgattat tgatgatatt
    12361 ggtggtatta tagtaattgc tttattttat agcgatttca atgccgaatc ggtaatatat
    12421 cttctttatg ctgcattttt tgtgattgtc cttgctcttg gaggcaaatt gcgtatcaac
    12481 ggaaaactat tttatgcttt ttttggtatt attgtatggt atttgttcct tcagtcgggt
    12541 attcatcctt ccattgccgg cgtagtagtt gcttttactg ttcctgccca tccgcgtatc
    12601 aacatcagaa cctttatacg tcagctgcgc agagatattg atttacttga aagtgtttca
    12661 cctaagccca cgaaggagga aataattctc agcaacacac aaattaaata tctttctcat
    12721 atcgaaaatg cagctgatca ggtaatcagt ccgctacaag attttgaaga caacctctat
    12781 gtattagtga attttattat tttgcctctt tttgcctttg ccaatgccgg tgtggttatc
    12841 aatctgtccg atttttattt gtcgggtatt gctctggcca tcatcatagg tttggtactg
    12901 ggtaaaacat tgggaatatt ttcctttaca tggcttacca tcaaatcggg cttatcgcgt
    12961 atgcccaatg gcatgaactg gcacagtctg tttggtttgt cattagttgg agggataggt
    13021 tttacagttg cattattctt aactgctcta tcataccctg tcggaagcga aatacttaat
    13081 caggcgaaat taggtattat tgcagggtct cttatttcgg gctttttggg ttactattat
    13141 ctgaagtttt cgatcaacag gggtgaaaaa aacaattatc gattgaaaaa aattcacagg
    13201 cttcataaaa tcatgtgttt aaacatttgt aagatgcctt ttgacaaaaa aataaaatac
    13261 tcaagggagt ttgccaaata ttttataaag cataatttgt aactatccga actattttta
    13321 tggaaaaaat cattatactc gattttggtt cgcaaaccac tcaattgata ggtagacgtt
    13381 taagggaact taacgaattt tgtgaaatac ttccctacaa caaatttcct gaacaaacaa
    13441 aagacataaa aggtataatt ctttccggaa gtcctttttc agtttatgat ccaaaagcac
    13501 tacaaatcga tttgaataaa attagaggga aattcccaat tttgggaatt tgttacgggg
    13561 cacaatatat tgttcactcg gccggaggga aggttcagcc tggaaatact agagaatatg
    13621 gacgagcttc gctcacctat gtggatgcaa cagacccatt aatgtcaaat attcctgtcg
    13681 gttctcaaat ttggatgtcc catagcgatt ctataaccga ggttccgcat aactttatac
    13741 aaattggaag tacgcaggat gtacaaattg cagctttccg tatcgaggga gaaaaaacct
    13801 ggggtgttca atttcatcct gaagtatttc atactacaga aggcctattg ctcttagaaa
    13861 attttttaaa tatttgcgat tgtcggcgaa actggactcc cgcttcattt atagaaacta
    13921 ccattcaaca attaaaagaa caattaaaag acgaaaaggt ggtactggga ttgtcgggag
    13981 gggtagattc ttcggtaaca gccgtgttat taaaccgagc cattggaaaa aatttgaccc
    14041 gcatttttgt cgatcatggt ttgctcagaa aagacgaatt tcgtgaagta ctcactaatt
    14101 atgaacattt gggtttaaat gttattggag tcgatgccaa agaaagattt tatgccgtac
    14161 tgaggggaat cgcagaccct gaaaaaaaac gtaaaattat tggagccgaa tttataaatg
    14221 tgtttaacga agaagctaaa aaaattgaaa atgtaaaatg gctggcacaa ggaaccattt
    14281 acccggatgt tatcgaatcc tattccattt cgggtaatac tattaagtct caccacaacg
    14341 ttggcggatt acctgaaaaa atgcacttaa aattatgtga acctctgcgt ttacttttta
    14401 aagatgaagt tcgcagtgtt ggtatcgaac ttggaatgac acctcaactt atcaaacgtc
    14461 accctttccc cggtcccggg ttggcagtca gaattttggg agaagttacc cctcaaaaag
    14521 ttgccattct tcaggaagct gactatattt ttattcaaga attgcgaaaa tgggacctct
    14581 atgataaagt ttggcaagcg ggatgtgttt tgctgcccat tcaagcagtt ggcgtaatgg
    14641 gtgatgaaag aacttatgaa aacgtggtgg cgctccgtgc tgttacttca acagatgcca
    14701 tgactgccga ttgggcaaaa ttgccttatg attttttggc caaagtatca aacgaaatta
    14761 taaacaaagt aaaaggtata aaccgggtag tgtacgatat cagttctaag cctccggcaa
    14821 ccattgaatg ggaataattt aaaaggtgga attttcaacc aaatcacagt aaagaattcg
    14881 ctgcacaagt agcagctaca aattgtattc acgatactgt ctaaacattg ttgcactttc
    14941 aaggtaaaat aaaactttta tgtgataatt caaacaagca tgatagacac ccatgcccat
    15001 atatattccg aagaatttga tgctgaccga gagaaagtta tagagagggc tctaaaaaga
    15061 ggcattgaac acataatctt accaaatata gatttgcaga gcttacagcc catgttgcaa
    15121 ctggaagcaa gccatcctga tatttgtcat gcagctattg gccttcaccc tgtaagcgtg
    15181 aaagaaaatt ttgcagaaac cctgaatttt ttaaaattca aattacaaca ccacaactat
    15241 attgctatag gagaaatagg catagatctg tactgggaca aaacgtatca aaatgaacaa
    15301 attgaagctt tttgtcaaca aatagaatgg gcattggaat gggaattacc agtaattatt
    15361 catgtaaggg attcgtttaa ggaaactatg caggctcttg aaccctacag aaataaaaag
    15421 ttaaggggtg ttttccatag ttttacggga acaatagaag aagcaaacca gatcataact
    15481 tttggaggtt ttaaaattgg tatcaatgga atactgacat ttaaaaattc tgaactttct
    15541 aaaactgtca gccaaattga gacagagtat ttggtattgg aaaccgatgc accttatttg
    15601 tcgcccgtcc cttttcgtgg aaagagaaat gagccggctt acctatccta tatatgtgag
    15661 caactcgcgc tgatacttgg gaaaacatcg gaagaagtcg agtttatcac tacccaaaat
    15721 gcaaagaaat tgtttaaatt acagccttaa cggtaaaaaa aaataaaaaa aacaagaatc
    15781 tgattataaa ataaaaacaa atatattgta tttcattttt ttttcttaac ttgcacccca
    15841 aaatggagag gtgctcgagg ggttgaagag gtgcgtctgg aaagcgtata tacggtgttg
    15901 aaccgtatcg agagttcgaa tctcttcctc tccgcaacat caaaacaaaa acaaaaaaaa
    15961 caagcagtca aaatttaaaa caaaaaatta gaaatcttaa aatttaaaga aaaatgaaaa
    16021 aggtatttgc aatcttatcg atcgtagcaa ttttttcttt cggaagcgct ctgcatgctt
    16081 cggctcaaga tacaagcgct cctcaaaccg agcagacagc cggcaatgat acggctgtag
    16141 cagctgcaca agcaatggaa caggtagaag cccctgcccc agcacaaggt ggtggcatgc
    16201 ataaggcttt aaaacaaaaa tttatcgaag gaagcccggc atggatggca cctattgctc
    16261 tctgcttaat tattggattg gctttgtgta ttgaacgtat aatttatttg aatcttgctt
    16321 ctaccaacac caagaaactc ttggatgata ttgaccaagc ttgggctaaa ggaggtgttc
    16381 aagctgctat ggaagtttgt agaaatacca gaggtccggt agcttcaatt ttttatcagg
    16441 gactttcacg gtacgatgaa ggaattgatg tagttgaaaa agctgttgct tcttatggcg
    16501 gcgtacaatt aggattgctc gaaacaaact taacttggat tagcttgttt attgctattt
    16561 caccaagttt gggattctta ggtactgtta tcggaatgat tgaagcattt gatagaattc
    16621 agcaagtagg cgatatttcc gcaacagtgg tagccggagg tattaaagtt gctcttttga
    16681 caacggtatt tggtcttatc gttgctatgg tgttgcaggt attctacaat tacatcctaa
    16741 ccattatcga aaacatgacc aatgatatgg aagactcttc aatttcattg ctcgatatca
    16801 ttgtaaaaca ttcgaaaaaa taaattcgta aaaacttaat aaggttatgt cgaaattcat
    16861 gcaaaaaata cctataattt cagcaatttt cctggtatcg gtatcggtaa tcttggtatt
    16921 gctgatttat gtagggggaa cctcaggttc gataaacaat agcgccggtg aggcaatgtc
    16981 agttccaaga tttactgatg cactaattta ctggtcatat tttcttgtgg gattagctgt
    17041 agttattaca ttgggctttg tagttaaaaa ttttttccaa tcttcaaaac aaagttcatc
    17101 atctattttt aagtctcttc tgcctatcat aggatttatt cttttgttcg ttgtttcatg
    17161 gctacttggc agtgatcagg aattgaaaat tatcggatat gaaggagcag agaacgttgg
    17221 attctggtca agatttaccg acatgatgat ttttccgatt tatgtaatgg caataggaat
    17281 tattttaaca atcataggta cttctattta ttccagtcta agaaaagatt aaaactgaat
    17341 tttgagttaa taaaagtttg actcttacta ataatatatt aaaatgtcga agaaaaaaag
    17401 aaaagtaggt caaataaatg ccagttcaat ggctgacatt tcatttttac ttttaatctt
    17461 tttccttgtt actacatcca tgaatgttgg aacgggtatc gcaagaagat tgccacctcc
    17521 gctgcctccc gatcaaaaac caccagacat ggacataaaa aaacggaata ttttcattgt
    17581 ccgtatcaac agccaaaacc aaattatggc acaaggagaa atattagata taaaacagtt
    17641 aaggtataaa acaaaggaat ttataaaaaa tgagaataat gaccctaatt tgccggaact
    17701 gattactgaa aatattccct tattgggaga agttacttat actaaaaatc atgtgatttc
    17761 acttcagaat gatgtgggga cgcaatacca agcttacata gatgtgcaaa acgaattgat
    17821 tgcagcatat aatgaactgc gcgatgagtt tgccatgcaa cgttttggga aaaaatacaa
    17881 cgagttaagc caagagttgc aagaagcagt tcaaaaaatc tatccacaaa aaatttcgga
    17941 agctgaaccc aaaaattatg gaggagtaaa aagtggcaag aattagaaaa agcgaaaaac
    18001 gagaaatgcc ggcattgagt acttcttctt taccggatat catcttcatg ctgttgtttt
    18061 tctttatggc tgttacaacc atgaaagaag taacctataa ggtcaggatt gatcctccta
    18121 aagctacgga aattcagaaa ttggaaaaca agtcgctcat tcgatatatc tatgtcggta
    18181 agcctttgga gcagtatcaa agaatttatg gtagcgaaac ccgaattcag ttgagtgata
    18241 tgtttgcaga tatttctgaa atagaagagt atatcattaa cgaacgtagt gcaatgaagg
    18301 aagaagatca gaatttaatg agtgtatgca ttagagctga caaagatact catatgggta
    18361 ttatcactga tataaagcaa gctttgcgaa gagcctacgc acttaaaatt atctatgctg
    18421 ccaatcagcg tacacagtat taatcttctt agtttttagt gttatccaaa taggctgtct
    18481 cataagtgcg acagcctatt ttttttgtgc tttgcaggat atagaaaaat ttattttaca
    18541 aataacaccg attggtaaaa ttcaaccgca tttttaccta aatcaaaaac attattttga
    18601 acgttttaaa ttgagatgag aatcaagatg agaattcgtg aaagtatcat tcctttttca
    18661 atcacatttt ttaaaatata ctcaataaac ggccggttgc agggtcaata attactattt
    18721 tgtcattacc tttgatcatg gaagacgata gcggaattac aatgccaaac tgtgggaaca
    18781 aaaattgttg agaaaaaaat gtgcttttcc catcggaaac agtaaccaat gtagcagaag
    18841 gaataacaaa gtttatgtat tctttgggtg ctttttgttt ggggttgtcc aatatggttg
    18901 gagatttagt aggaataaca ctaatatagt acggaacgcc actcaaatca tcagctggca
    18961 caagcccttt taaaaccgaa aaacgaaaaa ggacttcgtt gttcaaagct tgttgaggtc
    19021 gtaaatataa ggtatgtttt tgaatttctt tcgttgtact accaataaaa agttctgtta
    19081 actctttttc tttgttatcc aattcattta aaatggcact caatgatgtt ccatcagaag
    19141 ggaaatgatc aacgtctccg gtaagtaagt ttaatctgct ttcccgaatt ctatatatct
    19201 gtttggcagc tccttctgca agttttgcta ctgacccaac caacatatat tcttcaccca
    19261 gcggcaatat aacgttattg ctaagatttt cttttgtttc tgtaggtgca agcactgaaa
    19321 gttccggcaa agaggtggga ggaggaacat ttattccaca taataagccg ttttgatcaa
    19381 ccgagatgga cacaaagggc gaatttttat ccactctaac ggcataggtt ttcgatggat
    19441 cgggaacggc atttgaagtt acctgaatat cttttaaaga ataaactgtt ttttcttcgg
    19501 taattatatc tgaagtagcc aaatatcttt cagaataacg ataaaaaaca ccgggttttc
    19561 gggtaatttt ttccacgtca actttaatac ataattctgt catgggtaaa gagtaaataa
    19621 gcactgactc gtttttattc aatgtgaggg aagattgaca taacacgctc gaaataacag
    19681 aagccataag gaatgaaaca attatttttt tcataacact gaatttggtt gattgatgtt
    19741 gttaataaag caggaaatat ataaaatatt cagaaagatt tttcgacttt ttgaaaaaat
    19801 tgaccttaaa ggatgtttct ttatgtcatg ctataaaatc aattttattg aaattcattg
    19861 atttctgagc aaatatagaa aaacaggtgc aaattacaaa aaagaaaatc taaaaaatat
    19921 ttcagttggt aagaagaaag tataaatact ttagaaacat gttgttgtta ccgatttata
    19981 aaaaatctta tttctgttct acaacgcaga acgtttgtaa aagtataaat ttatttttat
    20041 tatctttgtc cagcttaaaa aaatcatttc ttggctgcaa aaaaggcaac atgccttact
    20101 tatatggaag aattttaatg tcgatttcat aaaaaagaat aaattgcctt attaatttta
    20161 tgggtaaaat tatatcgttg gccaatcaga agggcggagt tggtaaaact actaccgcca
    20221 ttaatttagc agcttctttg gcatcgttgg gtaaaaaagt tttgctggtt gatgccgatc
    20281 ctcaggcaaa tgcctcttcg ggattgggaa tagaaattca atcgctgaaa tataccattt
    20341 acgaatgttt gattgaaggt gtgtcgccac agcaagctat tcatggaacg gaaatagaaa
    20401 atttgtcggt gctcccttca catatcgatt tggttggagc agagattgaa atgttgaaca
    20461 tggaggagcg ggaaaaagtg atgcagcatt tgctacgccc gttaaaaccc gatttcgatt
    20521 ttattttgat tgattgctcc ccttcattgg gtttgataac ggtaaatgca cttacggctt
    20581 ccgactcggt gattattccc gtgcaatgtg aatattttgc attggaaggt atcagcaaac
    20641 ttttaaatac catcaaaata attaaaagca agttgaatcc ttcgcttgat attgaaggct
    20701 ttttgcttac catgtacgat aatcgtcttc ggttggcaaa ccaggtttat gccgaagtca
    20761 aaaaacattt tcagaatatg gtttttgaaa atgtcatata cagaaatgta cggctcagcg
    20821 aagcgccaag ttacgggaaa cccgttatgc tgtacgatcc cgaatcgaaa ggttcttcca
    20881 attacctcga tttggcaaag gaattgattg accgacaaaa aagttaatat agcttgcaat
    20941 aaaaaataaa aagaataaaa tctcgtttgc agttatggta aaaaaatcag cattgggaag
    21001 aggacttggc gcgttgatcg acaccgacga catttatacc agtggttcgt cttccattag
    21061 cgaagtggat gtggatttga tttttccaaa tcccaatcag ccacgttctc actttgatga
    21121 ggaatctttg gccgaactgg cttcttccat acgtgaactg ggggttattt cacccattac
    21181 gcttcggaaa aatgatgatg gaacgtatct gatcattgcg ggagaacggc gttatcgtgc
    21241 ttctaaaatg ttggggctca aatccatacc ggcctatgtt aaaacggcag ccgacgagca
    21301 agtggtggaa atggcactga ttgaaaacat tcagcgcgaa gatttgaatg ccattgaagt
    21361 ggctttggct ttttaccgac tgttggaaga atatcatctt acgcaggagc agcttagcga
    21421 acgagttggc aagaaaaggg ctaccattgc caattacttg cgtttacttc gtttgccggc
    21481 cgaaatccag gtgggaatta aggataaaaa aatcgacatg ggacacgccc gtgctatctt
    21541 gggattggaa gatcctgctg tacaacttca actctacgaa gctattctgg aaaattttta
    21601 ttctgtcaga aaagtggaag aaatggtcag actctacgcc gaaaccggaa gttttgagga
    21661 aaaacatgtt cgcgtttcca aacccaatcc gataagaaaa ttaaaagaat acgaagaatt
    21721 aaagaatcaa ctgagtaaaa ctttcggcac taaagtacaa tttagttgta atgagcaagg
    21781 aaagggaaaa atttccatcc cattttcaaa cgatgaagaa ttatcccgta tcatgggatt
    21841 gctggataaa atttaaacca aatttcaagt gaaaagattt tttctgataa cggcagaaat
    21901 atggtttatc tcccttttcg gaattgtact caatgctcag aatgtcaacg aaaaaagaga
    21961 gattgttcag gatagtataa atcggctgcc tgaaaaatct ttacaaaaag aaagccaagt
    22021 atcaattgat actgtacaaa agccggttta catgcccaat ccgtcgaaaa ttgtttggat
    22081 gggtgcagta gtaccaggtt ggggacaaat attgaacagg aaatactgga aattgccaat
    22141 tgtgtatggc ggattgttgg gatgttatta tgcctattca tggaatgcaa gccggtataa
    22201 ttcttatcat accgcttatt tggatattat tgattcagac ccgacgacaa acagttttct
    22261 ggatattttg ccggcaggat atactcttga atcaattggc gggaccggca gatatacaac
    22321 cattttaaaa agcggacaag atcaatttcg tcggcagaga gatttgagtg taattgtttc
    22381 ggtggctttt tatgctctta cgttggttga tgcttttgtg gatgcacaat tgtacggctt
    22441 caacattagt cccgatttgt cgcttgaaat caaaccaatt tatctaaaag ataatataac
    22501 tacatcaggg gggcttggca tgcaatgccg ttttaagttg caataattac cccgcattat
    22561 taggataaaa aatgaattta aaattttcca taattgttgt ttctttcatt tgcttttcga
    22621 taaattcagt tatttcacaa gctgccaaag atttcccgga agatacttta tcttccaaaa
    22681 tcgacagcat cattggaata cctgacgcat acggatatgc attagatcat ttgcttcatc
    22741 tatgggcatt gcagcgaatg gatactacag aatgcaattc gggtgagcca cccgcaccgg
    22801 tttccgattc tctatataaa tatcggctct cacaaatacc ttgtttgata gagcttcctt
    22861 ataataatat tgttcgttcc tttatagaac tttatactgt ccgaaagcga actcaaacag
    22921 aatacatgct gggcatcagc aattattatt tccctgtttt cgaacaagca ttagatgcca
    22981 ataaattgcc tttagaatta aaatttctcc ctatcattga atctgctttg caccctactg
    23041 ctatttcacg agctggagct gccggtttgt gggaatttat gattccaaca gggcgaatgt
    23101 atggtttgga aatcaacaca ttggttgacg aacggcttga tcctcgaaaa gcgtcctatg
    23161 ctgcagcgcg gtttcttaaa gatttatatg ccatttacgg agactggaat ttggtgattg
    23221 ctgcttacaa ttgcggtccc ggtaatgtga acaaagccat cagaagagcc ggcggaaaaa
    23281 ccgattattg ggcaatttac ccttatttgc cattggaaac acgaggctat gttcccattt
    23341 atattgctgc cacctatact tttcattatg ccgaagaaca taatttatgt ccagcagtgg
    23401 taaatattcc agcactaatt gacactttca tgatcaatca aagaatccat ttggaacaaa
    23461 tatcgtcagt gcttaatatc ccgattgaag agctccggtg gcttaatcca caatataaaa
    23521 aggacataat acccggtgat ttaaaaccct atgtcttaac tttgcctgta aattattcca
    23581 gcatttttat ggagagattg catgaaatag cttcttataa agctgatgaa ttaatcaata
    23641 accgtagaaa cgaaattgaa gctttcaggt caacttcttc ttctaattta ggggcttcca
    23701 aaaatgttac ttatcacaaa gtaaaaaaag gtgagacctt gagcggcatt gctgcaaaat
    23761 atggtgtttc agtctccaac cttaaaaaat ggaataacat caagggtagt aaaataacaa
    23821 ttggccaacg tctgaaaatt atcaaataaa attcaacatg gaaaaagtat attattccat
    23881 ttcggaagta gcagaaatgc tgaaaatcag tccttccaac ctgcggtttt gggaaaagga
    23941 attttcgcag ctgaagccaa aacgaagcaa caagggaaca cgtacttata ccaaagaaga
    24001 tattgaaatt attaaaatga ttcacttact ggtcaatgag cagaaactca cattgcaagg
    24061 cgcaaagcgt aaactttcgc tgaaaaagga gaccgtttcc aaacagcagg agttggtgga
    24121 acgtttacaa aatgtgagaa gggaattgtt gatgatcagt aaggctttgg atgaataggc
    24181 aatttacaaa tagaatataa aatgaaaaaa acgaattttt gtttaggctt gatagctgca
    24241 ttaattttga tgtcagcttc ggtttctgct gagaaaaagc caaaaaacgt aattctgatg
    24301 attggtgatg gtatgggttt agcccaaatg tattcggcaa tgactgccaa caacaatcaa
    24361 cttcagctcg aacggtgcac ttacaccggt ttttcaaaaa cttattcttc cgatcattat
    24421 actaccgatt caggtgccgg aggaacagcc atagcttgtg gcgtaaaaac aaaaaacggc
    24481 atgattggca tgacgcccga ttccgtaaaa acagaatcta ttctcgaact ctccgaacgc
    24541 aatcagttgt ccaccggaat agtagtaact tgtgcagtaa cccatgcaac acccgccagc
    24601 tttatcgcac atcagattag ccgcaatatg tatgaagaaa tagctggaga ttttcttcaa
    24661 acagatgttg atgtgttcat tggtggcgga ctgaagtatt ttaataacag aaaagaccag
    24721 ataaacttag tagaaaaact aaaaaaaaat aattatcaaa tagcatattc actcgacgaa
    24781 gctgtaaaag taaaatcggg aaaattggcc ggattgtttt atgagaatca ttgcccgccg
    24841 atgcctgagc gtggcgatat gctcccaaaa tcggtaatgg cagccataga tattttggac
    24901 aacaacaaga aaggtttttt cctgatggtg gaaggttctc aaatcgactg ggctgctcac
    24961 gacaacgaca agaaacagat cgtaaaagag atgttggatt ttgatgaaac cattggaaag
    25021 gttttggatt atgcccagaa agatggcaat acattggtaa ttattacagc cgatcatgaa
    25081 accggcggaa tgactatttt aaatggaaaa ttcggatatg acgaaatgga tgcccatttt
    25141 tctaccaaaa accactcggg tgttccggtg ccgatctatg cttttggacc cggagccgaa
    25201 cagtttaccg gtattatgga aaatacagcg tttaaaggga aaatagaaaa gttactcgat
    25261 ctaaaaaaat aatttttcat tattacttgt tgaacagtta aatttttaac agttcggtcg
    25321 taaatttttc tgatatattc attttcaatt taataaaaag acaatataat gataacaatc
    25381 gaacaactta aagacgttac agaacgcacc gaagcgttga ggaggtatct ttgacgtcga
    25441 caacaggcaa attcaaatag aagaagaaga acttcgtacg caggaaccca acttttggga
    25501 cgacccgaaa aaggcagaag ctcaaatgcg aaaattaaaa gagcttaaaa attgggtaac
    25561 cggttaccag gaagtgaaaa ccgcagcaga tgagctggag cttgcttatg atttttttcg
    25621 tgaaggcgtg attgaagaac aggaactgga cgaaacctat cggaaggctt tacaattgat
    25681 tgaacagttg gaaatgaaca acatgctttc tcatgaggaa gatcagttgg gggctgttct
    25741 taaaattgtt gccggagccg gcggaaccga agctcaggat tgggcggaaa tgctgatgag
    25801 aatgtatcag cgatgggcag aaagaaaagg ttataaatgg gagattacca acttccagcc
    25861 cggcgatgag gcaggaatta aatccgtaac cattcagata gaaggagaaa gagcttacgg
    25921 ctatctgaaa agtgaaaatg gcgtacaccg tttagtcaga gtctctcctt tcaatgctca
    25981 gggcaaaaga atgacttcct ttacatcggt ttttgttgtt ccgctgattg atgattccat
    26041 cgaaatagag attaatccgg ccaatctttc gtgggatact tttcgctcgt cgggagctgg
    26101 cggacaaaat gtgaataagg ttgaaaccgg tgtaagattg cattacacct ttaaacatcc
    26161 cgtaacaggg gaacagagcg aaatcgttat tgaaaacacc gaaagtcgtt cacaattcga
    26221 taacaaggac aatgctctgc gtttactcaa atcgcaactt tacgagcttg aacttcagaa
    26281 acgacgggct gaacaggaga aagttgaatc gaccaaaaag aaaattgaat ggggttcgca
    26341 gattcgcagt tatgtttttg acgaccgtag ggtgaaagat catcgaacca attatcaaac
    26401 ctccaatgtg caggcagtga tggatggtga tattgatgaa tttatcaaag cgtatttgat
    26461 ggaatttccc gactaagaga aaaacgttaa ttgctttttc ccaaattcca tattttgatc
    26521 gaaaatgttt gtcttcaaaa aaaatggagt aggggatatc tcttttttct atttaaaccc
    26581 agattactca ttaaccaatt taaagggaaa gtcaaagtca gaagagttgt tctatagtca
    26641 ggaaaactat gctcgccttt tttatttatt taaatggatt tgataactgg tttgctgttg
    26701 attttttcat gatatgcatt cgactgcaga agttctatat aaaagttcta tatctttaga
    26761 taaagttatt tttatacaag attaaaaccc ttttcctatt aaggattctg agttaaaata
    26821 agacatcgga caacaaaatt gcaactttca atattcaatt ttttttgcga aatttgcaaa
    26881 cttaaagaat ttaagtaaaa acggcaaagt gaaagaaact aaatacattt ttgttacagg
    26941 aggtgtagtt tcctctctgg gaaaaggaat tattgcagct tcgttgggga aactcctgca
    27001 gtcgagaggt tttaaggtga ccaaccaaaa acttgatcct tacataaata ttgatccggg
    27061 gacattgaat ccttatgaac acggggaatg ttatgtgacc gttgatggac atgaagccga
    27121 ccttgatctg gggcattatg agagattttt gaacattgag acccatcgtg caaataatat
    27181 taccaccggg cgaatttatc aaaatgttat tgaaaaagaa cgtcgtggag attatttggg
    27241 aaaaaccgta cagataattc ctcacattac ggatgaaata aagcgaaata ttaaattatt
    27301 gggctcaaaa ggcgattacg attttgtaat cacagaaatt ggcggtactg ttggcgatat
    27361 tgaatcattg ccctatattg aaagtgtccg ccagttgcga tgggaactcg ggaaaaattg
    27421 tttgattgtt catttgactt atgtacccta tatttcagct gctaaggaac taaaaacaaa
    27481 acctactcaa cattcagtca aagctttaca ggaacaaggt gtacaacccg atatcctggt
    27541 attgcgtacc gagcacaata tttcttccga tttgcgtaag aaagtggctt tgttttgtaa
    27601 tgtcgaaccc aattcggtta tgcagtctgt agatgtttct accatatatc gtgtcccatt
    27661 aatgatgtac gatgaaaaac ttgatgaagt agtacttaga aaaatggggt ttgacttgtt
    27721 gaaaatacct caggccaatc tttccaaatg gatacagttt gtggaaaaat tggaaaatgc
    27781 aaaagaagaa gtacacattg cattggtggg caaatatgtt cagcttcctg atgcttataa
    27841 atctatcatc gaatctctac ttcaggcatg tgcttataac gacagaaaat taaagctcga
    27901 tttaatattg tcggataaac ttaccgaaga aaatatagaa aaaaaacttg agggagtcga
    27961 tggtgttatt atcgctcccg gttttggtca aagaggaatt gagggaaaat tagtcaccat
    28021 caagtacaca cgtgaaaaaa acataccaac tttcggcatt tgtatgggga tgcagtgtat
    28081 ggtgatagaa tttgcaagaa atgtgctggg ttatgcagat gccaacagca ccgaaattga
    28141 cccgactacc actcataatg tagtggacat tatggaagaa cagaaaaata ttttgaattt
    28201 gggtggcagc atgcgattgg gagcatttcc ctgcaaattg aaaaagggga caaaagtttt
    28261 taagatttac ggaaaagaaa atatcagcga acgacatcga caccggtttg aatttaataa
    28321 ctattataaa gaagagtttg ataaagccgg catgatttgc agtggaataa atgcttcttc
    28381 aaatttggta gaaattgttg aagttccttc tcatagatgg tttatcggcg ttcagtttca
    28441 tcccgaatac agcagtacgg ttattaatcc tcatcctctt tttatcagtt ttataaaagc
    28501 tgccatcgaa tatagaaacg aatcgaagga aaaaactcaa aactcaaaat ttaaaacagc
    28561 tattaaataa ttaatgattg aaaatttaat tctcatttct cacttgttga atttcaaaaa
    28621 tatatcatgg ataaaaatac aattacaggt tttctattaa taacggttat tattgttgtc
    28681 tttgccttat tgaatcgccc caatcaggaa atgatcgaaa aacaaaggcg ctacaacgat
    28741 tcgatagcac aaattgaaaa agccaaagct gaatcggaaa ctgctattca agaagtcaat
    28801 tctttacttt ctgattctat taaaaccaca ctggattctt cggctgttat cagagatgct
    28861 tatggcgatt tcagtgttgc cgctttaggg aaagaagaat ttataacatt ggaaaacagc
    28921 gttttaaaac ttacctttac gaacaagggg gctcgtattt atgcagccga actaaaagat
    28981 tatcgtcgac atgattccct tcctctgatt ctttttcagg ggagtgaggc caatatggga
    29041 tttactctga taaccaacaa taacagaatt ctgaacacag ccaacttatt ttttgaagtt
    29101 ttacttccca tagaaaaaga tacattggga aatcaaatac ttgttttcag actaaaaact
    29161 accggcgaag gatacatgga tgtggtctac actcttcctc ccgacaatta tatgtttagt
    29221 ttcagcatta aagccaacaa aatgagcaat gttatgccgg cgggaactaa ttttttggaa
    29281 atgcagtgga ctacccgatt gcgtcagcag gaaaaaggtc ggcaatttga gagtcgttac
    29341 tcaaatattc agtataaata tctttcggat gatgtagaaa aattaagtga aagtaaaaat
    29401 gacagcaagc aaattaccaa caaactgaaa tggattgctt tcaaggacca gtttttcagt
    29461 aatgtgctta tagccgaaga tgcatttact tcgtcttttc tggaaagcaa ggttgaacct
    29521 gaggattctc cctatctgaa gtcgtacagg gccgatatga ccattccatt tgaccctacc
    29581 ggaaaaactt cgaccaactt tagaatgtat ttgggtccta ataaattcaa tatccttgca
    29641 tcttatgaca aaggagtagc caaagaagaa aagcttcaac ttcataaact gataccactt
    29701 ggatggggaa tttttggatg gattaaccgt tacatgatta ttcccatgtt taatttcttc
    29761 agtaaataca tcgggaatta tggcatcatc atcttattga tgacaattat cattaaaatc
    29821 ataatttttc ccatgacgta taaatcatat acatccagcg ccaaaatgcg agtccttaaa
    29881 ccagaaattg atgaaattta caacaaaata cctcctgaaa aagctatgga aaggcaaaaa
    29941 gcagtcatgg atctttattc caaagttggt gtaagtccca tgagtggatg tcttccggct
    30001 ttgttgcaaa tacctatcct ttttgccatg tttagcttct tccctgcttc gatcgaactc
    30061 cgtcaggaaa gttttctttg ggcaaaagat ctttcaactt acgattctat aattagctgg
    30121 aatgcttata tcccaattat ttcgaagtat tttggcaatc atttaagcct tttctgcctt
    30181 ttgatgacca tttcaagcat tatttccacc aagttgacaa tgacaactac tccaactacc
    30241 aaccaaccgg gcggaaattt aatgaagtgg atgatgtact tgatgcctgt catgtttatg
    30301 tttattttta acaattactc ggcagcatta agttactatt acttgctttc tactctgatt
    30361 accattattc aaacttttat cattcgctca atggttgacg aaaagaaact tttagcccaa
    30421 ctacatgcta aacgcaatca gaagcaacct gcgaagaaaa aaagcagttg gattgaacga
    30481 atggaaaaaa tgcaacgcga acaacaaaaa gcattgaaaa acaaaagata agagttttag
    30541 aaaataatat gtttaagcgt aaaattgcat aaaaaatttt tgtggtttta cgctttcttt
    30601 taaaattcaa tcatgacaaa aaaaatcaag ttttttattc tgttttttct gattatattt
    30661 cttttctcgt gtcaaaatca taaccgtttt cgcattaaca caaaaaagaa tgaagttgaa
    30721 gttcggatac ggcgttttga taaggatttg atcaacgttg acactacaaa tctcaaagca
    30781 gaaattagga aaatttacaa agattatccc gtttttttcc cggtatttat taacgaaata
    30841 cttaacgttg caccttccga caccgattca gttgctaaat taataggaga ttttctttgt
    30901 gatgccgctt ttcaaaaagt caacaaagaa gttctgaaaa cttttgctga tgtgcatgat
    30961 atagagaaac aactttcgat agcttttact tatatgcatc attattttcc cgagatcagg
    31021 ctacccgaaa tttatttttt cgtatccggc ttcaatcgtt ctatcattat ttacccttct
    31081 ttaattgctg taggggccga tttatactta ggagccgatt accctcttta taagtcgttt
    31141 acttatgaat acctgacttt caatatgcga cctgaaagta tagcccctga tgttgtttct
    31201 acgcttcttt ttagttattt cccaatggaa tcggaaaata atcgcttgtt ggacaatatg
    31261 ctgtataatg ggaaaatttt atttttgttg tcggtcttta tgcctgatgt agaaccttca
    31321 gtattgatgg ggtatactag cgaacaatgg caatggtgta aacgatttga aaaggaaata
    31381 tgggcaaaaa ttattgaaga caaagcattg ttttcttccg atatgaggct tataaattcc
    31441 tatttggatg aagctccttt tactgctcct gtatcacaag attctccagg tagagtgggc
    31501 acatggatag gttggcaaat cgtgaatagt tatatgaaaa aaaacaataa cgtcagtcta
    31561 cgtcagttag ctaatgaaaa taattatcag ctactgctcg agaaatcgga ctaccgtcca
    31621 taaaaaaagc ggttccgtca tgctttaatg acagaaccgc aaacaaaata aattaaatcg
    31681 aaaatttaat tatgcaggta tgattgcttc tgatggacaa acatcagcgc atacaccgca
    31741 atctgtacac acatccggat caataacata gatatcgcct tctgagattg catctaccgg
    31801 acattcagtt atgcacgatc cacatgcaat gcaatcttct gtaattacgt aagccatttt
    31861 tatatagatt tagtttaatg ctgcaaagtt actttttttt ttcaaaccaa aaaaatttca
    31921 caacacttat tcacttttga aatttcaaag tcgtaatttt tttttcaggt gagagacaga
    31981 ttattttgtt atgctcacaa acaaattttt cctttcatca gaatataatt tattttttta
    32041 gggtctgatg gaacccacat gtctgtatat tatattcatt cgtgtttgtg tatgcaatac
    32101 atgtgggttt catgttgaat tttaaaatct cattaagcct ttaattctga gatgggatat
    32161 tttatcttac ctttcttaaa gtaaattgtt gaaacatttc ttaaacattg gtattacata
    32221 ccaaagtcaa ttatcattgt ttggaattta tcacatccag cgctttcaat acagttttgt
    32281 cgcttcgata aagtaatgga taaaatcctt catcgcccag aatatttcgc gtaatatacc
    32341 ctttaatttg tgtcaaaagc aaattttttg agatgtttat ttctttttta ttggcttgaa
    32401 ttcctttttc ggaaacaaat tttacaaatt gatccaataa ggattgacta tttaagtaag
    32461 tgtccatttc gtgccaattc tttatttttt tcagtttctc tctgtttttg tcagaatatt
    32521 gaaaggcaaa ctgataaaca tatccgtaat tgaccacgtt attaaaatat ggtgtaaatt
    32581 ctgatgtatc caaaggaaca tatacatcgg gcataatgcc gcctccgcca tatacaattc
    32641 ttcctccaac cgttttgtat cgcaaagaat ccgggtggaa aatactgtcg ttaatttcat
    32701 gcaacagacg gttgataaat tctttttcgt attcttccgt attaccaagt ttataaggct
    32761 tttgaatact tcgaccggaa ggggtataat agcgtgccac cgttaggcga acagccgaac
    32821 catcagacaa attaaactgt tgttgcacca atcccttacc aaaagtacgt ctgcctacta
    32881 acgttccccg atcgttgtct tgaatagccc ctgcaaaaat ttcacttgcc gatgcagaaa
    32941 attcatcaac gagtactacc agcggctccc ttgtacagct accctttcca tctgatttgg
    33001 cttcaaagcg cttataggct ttcccttgtg cataaacgat aagtcggtta gccggcagaa
    33061 attcattaat catattaatg gactgatcca taaatccgcc actattgcct cgcaagtcaa
    33121 ttatgaattt atcagcacct tcgtttttta atttggctat ggcattcaga aattctccat
    33181 aagttgtttc ggcaaatttg ctgactttaa taaaacccac acttggctga acaatataag
    33241 caatatctac ggaatttaca ggaatttttc ctctggttat ttcataatac aacacttcct
    33301 ttgtttggtt tcgtttaatg cccaacttaa ctttcgtacc ttccggtccg cgaagttttt
    33361 tcattacttt ttcattgtta atactttttc ccacaaaggt ggtgtcattt acttctacaa
    33421 tgcgatctcc gggcattaag ccagctgctt ccgaaggacc tccgctgatc acggatataa
    33481 tcatgactgt atcgttctga atgttgaatt gtacgccaat accactaaaa ctaccttcca
    33541 attcgctatt tgccaaagaa atatcttctg ccggtatgta taccgaatgg ggatcaagtt
    33601 ttgcgattat atcttgcatg acgttttcag tcatttcatc taagttgagt gtatcgacat
    33661 aattagtatt aataatctga agcaattcat ctactttgga agcattacca aaaccgaaaa
    33721 aaatgtttgg tgaagacgag aaaaaagccc tctgagccag taagttaccc agaataagtc
    33781 caaaaattac ggctaagaat acgatgaggg gtgcaagata attttttttg ttgcgatcca
    33841 taaataaaaa ttatttttta tgttttccaa aatatttatt taaatgacat tgaaatgatg
    33901 gtaaatttca tctcttattt atgaaggttt ttgttctcgc cggtaatttt catttttatc
    33961 aaacgaacta attctgcttc aaaaaatgat tatctcataa ataagtggat aaaatggtgg
    34021 aaaaattacc gaaaaaattt acttaaaaga tgaatacaaa attagagaat taactttgtt
    34081 tttctgtaaa cattttacta aaaataacga ttttgaccga cttttgttct ttgaattgtt
    34141 tttctttgat tttatacaat ggtttcctat atccgttggt ctactttctc tgtaaaaaaa
    34201 attattggca aaaacatttt cattatttta aataaaaaca ccttatattt ccacgttaat
    34261 tattaaattt gcgaacttaa ttataaaacc agaaaaaaaa tgaaagtacc tgaaaatttg
    34321 aaatacacca ccgaacacga atggatcagg gtgcaaggta atactgcatg ggtgggaatt
    34381 accgattttg ctcagcatga attgggcgaa atagtatttg ttgaggtaga aaccttggga
    34441 gaaaacattc atgccggcga agtttttgga acagtagaag ctgtcaaaac agtttcggaa
    34501 ctttttttgc caataagtgg tgaaattctt gaatttaata aagaactcga aaacaagccg
    34561 gaactgctca acgatgatcc gtatggcgaa ggctggattg ttaaaataag ggtcaccgat
    34621 ccttctgaac tggaaaaatt actttcggca gaacaatatg ccaagttgat tgcttgaaca
    34681 agaagtttta taaatcaatt ttttaaaaat gaaagcaaga gcaagtatta tcatgggtag
    34741 cacctccgat ctgaaaataa tggaaaaggc tgccaagctt ttggatgaat ttgaaattcc
    34801 ttttgaaata catgcgttat cggctcatcg tactccaaag gaggtagaag attttgctct
    34861 tcatgctgaa aaaaggggta ttgaaataat tattgccggg gcaggtatgg cagcacattt
    34921 accgggtgtt atagcttcga tgactacttt gcctgtgatt ggtgttccaa ttgaagcttc
    34981 tttaaacggt atggatgcct tgttttccat cgttcaaatg cctccgggta tacctgttgc
    35041 tactgtaggg atcaatgctg cacaaaatgc tgcaatttta gctgttcaaa tattggcttt
    35101 aaaagacgaa aaaatcaatt ccaaattaaa gctgtacaaa gaaaatatga aacaaaaaat
    35161 tgttgaagca aacaatcaac tcaaagaaat aaaatatact tataaaacaa attaattagc
    35221 tccgtaaact atcactgaga aagctgaaat tatcattaaa gtttttattc ctttttgttt
    35281 cattttctgc gaagtattat cactatttca cgaagtgtga tgtaaattaa aaagttgtat
    35341 aaaaaattaa aatcttattc tttcgacaag gataagaaaa ttgattaaga ataattttcc
    35401 acttttgaat aggttttgtt cattttttag catctaccct gagggtaaag aaaaccattt
    35461 tataaccccg tcgcccgaag aaaacgacat tttatttttg gttttttccc ctatttcccc
    35521 tttttcaaga taaccaacac tgtatctttt tgtttccagc tttgttgtaa tattgcccaa
    35581 atttcgtacc tttgttctat ggctaaatgc cttcatcatg gcaatttttt aagaggaaga
    35641 gaccaaagta atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaaataat
    35761 tctcttttgc ttttatttcc cacgaaaaaa ttgcatgaat tacttgaatt tacgtttgtt
    35821 ttataaagag tgttttttaa aatatgacta cctatccttc tcagtctgtt tttagtttat
    35881 accgtcgcat ggtaacttct gaaaagaaca tgttttcttt tcaggaaagg aaggccatca
    35941 aacaaattct gaaggaatac tatctactcc atccgcagca attgacatta ccgggccatt
    36001 ctatcgaaga caaattgtat attattcaga ttctgctcaa tgaaatagga ttgggaaaag
    36061 cttctgttct gagtgtgctt tttatggaac cgatcaagaa caattatttg actgtttcag
    36121 aaattgaaca aaaattcaaa actccgcttg actccataat aggcggaatg cagcgtgtag
    36181 aagagcttta cgaacgcagt ttttcgctgg agacagagaa tttcagaaaa cttttattga
    36241 cttttgctca agatgttcgt gtcattttaa tgatgatagc cgagcgactt tattttatgc
    36301 gaatactgga taaatttcct gcagatatgc agcaaagcat ggctcgcgaa tgctcgtttc
    36361 tttatgctcc gctggctcat cgtatgggac tttattccat taaaagcgaa atggaagatt
    36421 tgtcgctgaa atatacaaat cgggaaattt atcgtgaaat tgcccaaaaa ttgaatgaaa
    36481 ccaaacgttc tcgcgacaag tacattgaag aatttattac ccctttgaag gaaaaacttg
    36541 gcaaggaaat taaatatccc ttcgaaatca aaggtcggac gaaatcaatt tattccattt
    36601 attccaaaat gctgaaacag aatacgcctt ttgaaaacat ctacgatctt tttgcaattc
    36661 ggattattct ggatgtaccg ttggaaaaag aaaaaatgat ttgctggcag gtttattcca
    36721 ttgtaaccga catgtatcag cccaatccga aacgtttgcg cgactggctt tccataccga
    36781 aatcaaatgg ttacgagagc cttcatatca ctgttttggg gcctcaaaac aaatgggtgg
    36841 aaattcagat tcgcactacg cgtatggacg aaatagccga aaaaggattg gcagctcatt
    36901 ggaaatacaa gggaataaaa ggtgaaagcg gcatggacga atggctgaaa aatattcgcg
    36961 atattttgga aaatcccgac ttgaatgccg tagattttat ggatgaattc aagttgaatc
    37021 tttacgatca ggaagttttc gtttttactc cggcgggaga tttgcacaag ttgccaaaag
    37081 gcgctacggt actggatttc gcttatctga ttcacagcaa tttgggaagt aagtgtattg
    37141 gagccaaaat caacaaggaa agaaatgtgc cgataaaata tgtgctgaaa aacggagatc
    37201 aggtggagat cctcacatct ccctcccaaa agccaaatcc ttcgtggctg aacatagtag
    37261 taacttcaaa ggcgaaatcg aaaattcgtc aggcgctgaa agatatagag tttaaagatg
    37321 ccgaaattgg tcgggaaatt cttttgcggc gtttcaaaaa ctggaaaatt gagttggaag
    37381 aaggaaagct ttcgcgattg gccaagaaaa aaggttataa aaccgtcagc gatttttatc
    37441 aggccattgc acatgaaaaa atcgacatgc tgctcattcg cgatgcctat ctcgacctcg
    37501 accggcatga aacgggagca gaacaggctg aacctcggag tgccgaaact ttttcaaaag
    37561 aaactcctgc cgaagaactt tcttcaaaag aagatgttct gattatcggc caaaatctga
    37621 aaaatgtgga ttacacactt gccaaatgtt gtcaacccat ttacggagac gacatttttg
    37681 ggtttgtatc cgttggcgga ggcatcaaaa ttcatcgaac cacttgcccc aatgctcctc
    37741 agttgatttc aaaatacggt tatcgaatta tcaaggctca atgggccgga cgctcaggag
    37801 gaactcagta tccgattaca ctttatattg tcgggaaaga tgacatcggt attgtaacca
    37861 acattacttc acttatttcg aaagagaaaa atattttgtt gcgttccata tcggttgatt
    37921 ccaatgccgg tgtttttcag ggaaacctta caataatggt aaacgatgcc aatgatttgg
    37981 acagtatcat aaaaaaaata aaacaggtaa aaggtgttct gagtatttca agaaccaaaa
    38041 gtttaaccca aaacaaccag taaaatttta taaacaatgt tttcaggaat aattgaagaa
    38101 gctgccccgg tagtagcttt aaaaaaagaa gccggcaatt tgcacattac catgacttgc
    38161 tcgttcgtaa acgaattgtc ggttgatcag agtgtttcgc acaatggcgt atgcctcacc
    38221 gttgtttcca aaactcccga tacctacacc gtaacggcta tccgtgaaac gttggaaaaa
    38281 agcaatttgg gattgctcca agaagggagt aaggtgaatc tggaaaggag tttgctactc
    38341 accgaacgtc tcgatggaca tattgttcag ggacatgtcg atacaacagc agtttgtacc
    38401 gatatttctg aagctgatgg cagctggtat tttacttttc aatatgatgt ggacagggaa
    38461 ttggcaaaaa gaggttatgt taccgttgac aaaggttcag tggcggtaaa tggagtcagt
    38521 cttaccgtgt gcaacccgac ggaaaatacc tttcaggttg ccataatccc ctatacttac
    38581 gaacacacca attttcatca aatttgcgtg ggaacgacag tcaatattga gtttgatatt
    38641 attgggaaat atatcagtcg attgataaag ctataaaata cgccctgatt ttttgtttta
    38701 tcaaactaaa atcgatggca attgttatat aacattgcaa aatccattta gaatttggta
    38761 atggaaaatt tcaggaaaca gataaaaatt tcatttttca atcccattat tcattttctt
    38821 ccgcttctgc tgtttttggt aacagctatg tatgccggaa gaacggtggc gaattatgtt
    38881 tccattttcg tccttcttgt gtcggcggta tatgttttta ttgcttataa tcagatattt
    38941 atttggtttt tcctgtcgtg tgtcatcttt ttggcggtca atattgttgg cagtataatt
    39001 cgtttgccga taatttttct gagttttcga cctttgttgg atgaagcgtt gacattggta
    39061 tttttgggag gaatgatgtt gctgcaaaaa cgcataaaaa aaggaattat ggcactcatt
    39121 cccaaaactt tgccaatgaa taacaatctt gatgaatatt tcggttttat ccgaactatt
    39181 ttcttgataa ttttgggata tgttttggct accggttttt tctatgcttt tcctacgcag
    39241 cgggaaacgg cttatttgca actggtgcag gcaatttatg tcggaattct tttctttgtt
    39301 ttggttttcg aatcgcttcg ggttcaaatt attcgtgcac gcttgctgaa agaagaatgg
    39361 ttgccgatag tcaattcgca gggaaaaatt attggcagcg tgcaacatca agccagtttg
    39421 ggagaaaagc gaaaattcat gcatcccatt attcgtgtac atattattca ggatggcatg
    39481 attttgctca gaaagcgtcc tgctgacgat ttgtcctatc ctttacattg ggatacggct
    39541 atttcaaatc atatccgcat gggagaaaca gttgaagact gcattaaaag gacggctcaa
    39601 gagctatatg gtttgaccga gttaaaatct tttttccttt ccaattatat tacccacaca
    39661 gattttgaac agcattatgc ttttcttttt attgcctgca aaatgaataa aatagacctc
    39721 cagtcggaaa aagtgaaaca ggcaaaatgg tggacgctgc aacaaattga agaaaatttg
    39781 caggaaggaa tttttaccga aaactttctg atagaatttg atttgttgaa aaagagtgga
    39841 gttttggaga ataatatttg cgattgtgcg tgcaaattga aagaaaccat tttcaaagct
    39901 cctcatctgt ttcggtaaaa gcaaagcata aataacttaa tgatgagatt atattttatt
    39961 ttgctcctaa tagcatttgt ttcctgtggt gggcattcca actcggaatt taagctgcaa
    40021 aattcctttc aaattaagct gcaggattcg atttgtatca atgctaaaga ttgtttttat
    40081 tttagtaccg acagtaacct ttatcggatc tttatgtatt tttcaaatgc agaattgaaa
    40141 gaaaaaaaaa taattgatac cgtagatttt agcccatata aatcaaagat acatagcttt
    40201 caatcggaaa ataatgagtc ttatgttgtt ttatgggaaa cagaatacga attttatcct
    40261 ttaatttatg tctactttat tacagcaggt aaaatagtaa aaattggaga acttttaatt
    40321 tctttaccct gtcagtcgtg tgagagtttc gaatatccaa taaaagatat tcgaatgctt
    40381 caaaagggaa tgaatattga aatctcattt ttaaaagatg taaattataa gccaagtaac
    40441 ggtaatgaat ggcatttgta taaagccggt atgttaaaat gcatttttaa tgctgaaaca
    40501 aacaaattaa aatagatctc atcattgaat tagtgttaag ttagatgaca aaaacaattt
    40561 atttcactta aagataaacc tatattattg attatctatg ttttacatta tataaagata
    40621 ttttgtctct cgctggcttc agtgatttac cactgccaaa atcccctgat ttgtaaggtt
    40681 ttggagagtg gaaggagtgt tcttgttatt aaggtttgag caaaataagg ttgaaggaaa
    40741 taccacaggg caatcttatt ttttggtctt gacatg
//
LOCUS       DAOSCH010000008        40230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_752_length_40230_cov_6.594200, whole genome shotgun sequence.
ACCESSION   DAOSCH010000008 DAOSCH010000000
VERSION     DAOSCH010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 40230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40230
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_752_length_40230_cov_6.594200"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="PLZ54_01480"
     CDS             complement(<1..492)
                     /locus_tag="PLZ54_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65645.1"
                     /translation="MKKQFVLLSFMAVVLLTGCNIDGDSNYTPAIQFLAYPYTQHGDT
                     LNIKTSDESGVYLLDSIQVGDTVFFQLGLNAYTNNLVSFRLTKTVDSAGDFLFPPVAS
                     LDSVFSHTNSDYGQGKFVFLSGFNAVYFPFHFAAKSATNDEILQFSLVSDAQFDDVYG
                     SNVT"
     gene            complement(572..644)
                     /locus_tag="PLZ54_01485"
     tRNA            complement(572..644)
                     /locus_tag="PLZ54_01485"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(609..611),aa:Pro,seq:cgg)
     gene            847..1575
                     /locus_tag="PLZ54_01490"
     CDS             847..1575
                     /locus_tag="PLZ54_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOJ65646.1"
                     /translation="MFKEAIQNYLQRHDYKSFYPKAVFFDMDGVLIDSMKNHSRAWMQ
                     AMKESNLPFTEEDAYMHEGCIGATTINDTFMKHLNREATEEEQKQIYSAKSRIFESLE
                     PIEKMPFAYELLKKLKSEEMKIYVVTGSGQPKLIDSLNTLFPEIFTKETMITGFDVKE
                     GKPSPEPYLLALKRSGFQPWETIVVENAPMGILSASKAGLFTLVVNTGPFDDDFLYSY
                     GADLVLDSMEHLYNEWDNFRKSWI"
     gene            1577..2608
                     /locus_tag="PLZ54_01495"
     CDS             1577..2608
                     /locus_tag="PLZ54_01495"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOJ65647.1"
                     /translation="MKKTGLELFLSPVFLSLILFFSSTVIFAGETDSASFHPQIEGTI
                     RAKYEYNTSLNASRFQVRNARFSVNGNFNSLVSYKTEIDLSDEGQTKMLDAYIRLQPQ
                     KWFTFTIGQQKIPFSTDNLRPPHLLYFANRSFIGKQLTALRDVGTTLSFVNNKTLPFE
                     FITGLYNGEGLYNQKIWQTSFSYAFRLVLHPCENLHFSLNHNSIRPEEMRMNLYDLGG
                     ILQLKNWHLETELFYKTYQQNFFPTTKGFFVFSSYDIYLHNKESIKIIRPLIRYDSMT
                     DNNKGYKSENGSYESDDMARSRITGGITLCLDKPFLNDIRLNYEQYFYKKGIQNNDNK
                     FVVEFVLRF"
     gene            2681..3994
                     /gene="hisD"
                     /locus_tag="PLZ54_01500"
     CDS             2681..3994
                     /gene="hisD"
                     /locus_tag="PLZ54_01500"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOJ65648.1"
                     /translation="MQVIKYPAREFWSTLLSRPAIDRKSLFEKVGSIVSEVKQNGDKA
                     VKKYTLQFDKVGIEELEVSQEEIEEASLQIPNQLKEAIEMCRRNIWKFHSAQQHDLPE
                     IQTTPGVVCWQKAMPIEKVGLYVPGGTAPLFSTVLMLGVPAQIAECKEIVLCTPPDKE
                     GKIHPAVLYAAKVAGIHRIFKIGGIQAIAAMAYGTETVPKVYKIFGPGNQYVTAAKQL
                     IALDGIAIDLPAGPSEVMVIADESARPGFLAADLLSQAEHSVDSQSILITVSENLVEP
                     VLLEIEEQLNRIPKKEIALKALENGKIIVLQSLEEAVAMANEYAPEHLIISTRYSLGV
                     ASNISNAGSVFLGHYTPESAGDYASGTNHTLPTNGYAKAYSGVNLDSFMRKVTFQQIS
                     REGLSNLSNAIILMAENEGLYAHSNAVKVRLDKEESEEKPENRLC"
     gene            4070..5104
                     /gene="hisC"
                     /locus_tag="PLZ54_01505"
     CDS             4070..5104
                     /gene="hisC"
                     /locus_tag="PLZ54_01505"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOJ65649.1"
                     /translation="MKINELVRENIRNMQPYSCARSEFKGEASVYLDANENPYNSPFN
                     RYPDPLQTELKKEISKIKKVPVENIFLGNGSDEAIDLLYRAFCEPKIHNVVAIEPSYG
                     MYKVCAEINNVEYRPVLLDENFDVEAYKLMDASNDKTKIIWLCSPNNPTGNSLNFSEI
                     NKLLSWFRGIVVIDEAYIDFSTKKSLLNYLTLFPQLIVLQTMSKAWGNAGIRLGMAFA
                     DPEIIGILNKIKYPYNINYLTQQHALQALQNPMQVEKWVALILEEKEKLIKELQKLKL
                     VEKIYPSDANFILVKVENANAIYRFLIQNGIVVRNRSNMPLCNNCIRITVGTPEENKA
                     LIDTLKKFHV"
     gene            complement(5164..5237)
                     /locus_tag="PLZ54_01510"
     tRNA            complement(5164..5237)
                     /locus_tag="PLZ54_01510"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5201..5203),aa:Met,seq:cat)
     gene            complement(5571..6581)
                     /locus_tag="PLZ54_01515"
     CDS             complement(5571..6581)
                     /locus_tag="PLZ54_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOJ65650.1"
                     /translation="MEETKYMPADFKTDQQPRWCPGCGDYAIFNALVKTMAELGVSPH
                     ETVIISGIGCSSRLPYYTNTYGFHTIHGRGACVATGVKVANPALTVWQVTGDGDCLAI
                     GGNHFIHSVRRNVDINVLLFNNQIYGLTKGQYSPTTKKGFVTKSSPYGTVERPFRTAE
                     LTFGAGGTFFGRLLDVDLKTSQMVMKAAALHKGTSVVECLVNCVIFNDGAHGWLAEKE
                     TRDDRIVVLEHGKPMIFGKNKDKGLILEKSELKVVTIGENGITDKDILVHDAKCKDTF
                     LHTKLALMEGPEMPIAMGIIRDVEEETYEMAMENQINEIKKQTSVKSFDELIDACEQW
                     EI"
     gene            complement(6615..8459)
                     /locus_tag="PLZ54_01520"
     CDS             complement(6615..8459)
                     /locus_tag="PLZ54_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOJ65651.1"
                     /translation="MNKAQTIQLDNIVIRFSGDSGDGMQLTGNLFAQLSAELGNEIST
                     FTDFPSEIRAPQGTLGGVSGFQVHVGANKIYTPGDEIDVLVTLNPAALKVNAKNLKKG
                     SIVIFDLDSFQPSDLKKANFSTDNPFEELSLSDTIQLIPVPLTTLTKSSLEDFGFDNK
                     TVLRSKNMLALGLVCWLFNRPLDKAIHFLENKFRKKTQILQANIKALTDGYNYGENLH
                     LTVSNFVIEKSHEMPKGKYTSINGNKATAWGLIAAAEKAGLQLFLGSYPITPATDIMQ
                     ELALQKGIGVKTVQAEDEIAGITMAIGASFAGCLAATSTSGPGMSLKTEGIGLAVMAE
                     LPLVIVDVMRSGPSTGMPTKTEQGDLLQALYGRSGESPLAVLAAPTPDSCFHYAFMAS
                     KIALENMTPVILLTDAFIGNGSSLWRLPKLADLPEIHPHFVPSNMENYKVTTRNEDTK
                     IRYWSIPGMKGFQHCNGGLEKDYTTGNLSTDPENHEKMVLTRKQKVDNIVNVIPELEI
                     EGNTDADLLVVGWGGTYGHLKSAVDNLNNKGYKIALAHFTYLFPLPANTENVIGKYEK
                     IVVCELNTGQFASYLRSKIPGKTFTQFNKIQGQPFKTEELENHFISLL"
     gene            complement(8520..9926)
                     /gene="asnS"
                     /locus_tag="PLZ54_01525"
     CDS             complement(8520..9926)
                     /gene="asnS"
                     /locus_tag="PLZ54_01525"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOJ65652.1"
                     /translation="MELMKRTKIVDALKKIDFGEDICVKGWVRTRRGNKNVSFIELND
                     GSTVHNIQIVADNNKLGIDFLKPITTGACIGVTGKLAQSLGKGQSVEIQAEDIVIYGT
                     ADPETYPLQKKEHTMEFLREIAHLRPRTNTFGAVFRIRHHMAMAIHTFFHERGFFYFH
                     TPIITGSDCEGAGQLFQVTTLNLSDLPKAENGCIDYSNDFFGKQTGLTVSGQLEGELG
                     ALALGAIYTFGPTFRAENSNTPRHLSEFWMIEPEVAFNELEENMQLAEDFVKYCVRWV
                     LEKCSDDLEFLNNRYDPELIDRLKFVIENDFVRLTYSEGIKILEDAMAKGQKFEFPVG
                     WGIDLQSEHERYLVEKHFKKPVILTDYPKNIKAFYMKLNDDGKTVRAMDVLFPKIGEI
                     IGGSQREDDYEKLKARAKEMEVPDKDIWWYLETRQFGNAPHSGFGLGFERFLLFVTGM
                     SNIRDVIPFPRTPKNADF"
     gene            complement(10116..11630)
                     /locus_tag="PLZ54_01530"
     CDS             complement(10116..11630)
                     /locus_tag="PLZ54_01530"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOJ65653.1"
                     /translation="MRVKVLIKVSTFCLALLFLGCAGQSNKEGYSRVTGWKYNDEKGA
                     GFAVRKDFKTNVPPGMVEIEGGSFTIGEKGEVVTAPRNSRRRRITVSSFYMDQYEIRN
                     VDWREYTHWMNLVFGKTAPKLVEKTYPNGKVWREELAYNEPYIEHYFTHPAFDQYPIV
                     GVTWEQAMDYCSWRTDRVNELALVKAGIIAPPDFASLKNETNFDNIINNFVFNTQKYL
                     KQASYQPPVGKNPKKDLTGEIRKVDMSDGILLVSDIRLPTEAEWEYAAYGLKAGKDGF
                     IPEGKIYPWNGHQVRNPSRKQLGQMMANFVRGRGDMMGTAGSLNDRATITAPVNSYYP
                     NDFGLYNMAGNVNEWVLDVYRATSFEDVAEYNSFRGNMYLTPIVEKEDEVGNKIFKID
                     SLGRIATTVLEGDDVRNYKDGDLTSKISTDFQLYADSSDIAAITNMKKIDPTDVLAPQ
                     ISDKTRVYKGGSWKDRIYWLNPSTRRYLDQDQCSNDIGFRCAMSKMGNIEPVKR"
     gene            11991..12842
                     /gene="folP"
                     /locus_tag="PLZ54_01535"
     CDS             11991..12842
                     /gene="folP"
                     /locus_tag="PLZ54_01535"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOJ65654.1"
                     /translation="MFLNLNGRLVDVSSPVVMGIVNVTPDSFYTGSRFLSEKSILTSV
                     DNILSNGGTIVDVGAYSTRPSAESVSVEEELKRLSRALEIILKQFPNVILSVDTFRSK
                     VARYVIENFHVAMINDIGGGTLDELMFETVADLRVAYVLMHMRGTPQTMQTLTHYENN
                     VVSEVFSFLQKGLAQLHLLGVKDVIVDPGFGFAKTLEQNYELLHKLHYLKELNAPILV
                     GLSRKSMIYNLLRLSPEEALTGTIAANMLSLIGGASILRVHDVKEALQTIRIFEQYQN
                     FSTFIND"
     gene            12854..13666
                     /gene="cdaA"
                     /locus_tag="PLZ54_01540"
     CDS             12854..13666
                     /gene="cdaA"
                     /locus_tag="PLZ54_01540"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOJ65655.1"
                     /translation="MFFQIEFKDIVDIFLVAFLLYEIYKLLRNSGAINIFIGILTFIV
                     CWFFVSYVFKMQLLGEIMDTVVNVGAFALIVLFQDEIRRFFTQIGTKRENRFLSKMKK
                     IFGESEYDKKETDFDLLQVVLACRNMSRTNTGCLIVITRRNALEFYVQSGELIEAKIN
                     SRLIESIFFKNSPLHDGALIISRHTIKAAGCILPVSQNQSIPKRLGLRHRAALGITEH
                     TDAVAIVVSEETGHISWAVNGNLTHNVKPEELEYFLNTQLQGSNKVEEEKIK"
     gene            complement(13735..14727)
                     /locus_tag="PLZ54_01545"
     CDS             complement(13735..14727)
                     /locus_tag="PLZ54_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HOJ65656.1"
                     /translation="MVHYTELGLVNSRELFKKAIEGKYAIPAFNFNNMEQLQAIISAC
                     VETKSPVILQVSSGARKYANQTLLRYMAQGAVEYAKELGYNIPIVLHLDHGDTFELCK
                     SCIESGFSSVMIDGSHLPYEENIALTKKVVEYAHQFDVTVEGELGVLAGIEDDVVAEH
                     HTYTDPDQVVDFVTRTNVDSLAISIGTSHGATKFKPEQCTRDENGILIPPPLRFDILE
                     EIEKRIPGFPIVLHGASSVPHEYVEKINKYGGALKDAIGVPEDQLRRAATSAVCKINI
                     DSDGRLAMTAAVREVFATKPAEFDPRKYLGPARDELKKLYMHKTVNVLGSAGKA"
     gene            14963..15382
                     /gene="mscL"
                     /locus_tag="PLZ54_01550"
     CDS             14963..15382
                     /gene="mscL"
                     /locus_tag="PLZ54_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOJ65657.1"
                     /translation="MKLWKEFKEFAMRGSVIDLAIGVIIGSAFGKIVSSLVADVIMPP
                     IGLLISGINFTDLKLVLKPAEMVNDVQTPAVTLNYGNFLQVAFDFLIVAFVIFLLIKG
                     INNFLKKKEEKPVPEATAQPSKEEILLTEIRDILKEK"
     gene            complement(15452..15733)
                     /locus_tag="PLZ54_01555"
     CDS             complement(15452..15733)
                     /locus_tag="PLZ54_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65658.1"
                     /translation="MQTINQLFQLLLKDIQEKMNDEFDRNFERNTFFAEAWNPNKHNS
                     IGLLMIWTGALRKSLRSEKIDIYSIWLQSLLPYAELHNCGETFNRTKRN"
     gene            16584..18125
                     /locus_tag="PLZ54_01560"
     CDS             16584..18125
                     /locus_tag="PLZ54_01560"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOJ65659.1"
                     /translation="MTHQNGLDVKTLENWLWEAACKIRGPIDAPKYKDYILPLIFLKR
                     LSDVFEDEINRLSQNYGSREIVEQFLSNDHNLVRFYLPPETRWEAIAKHTTGVGEYLT
                     NAVRKIARENPKLQSVIDIVDFNATAGGQRIISDEVLKDLINVLGKYRLGLDDVEPDI
                     LGRAYEYLLRKFAEGSGQSAGEFYTPSEVAVLMSYILDPKPGDEIYDPCCGSGGLLIK
                     AFLRSKEKFDDNPSFEPVRFYGQEILHATYAMAKMNAFIHDMEAEIALGDTMNRPAFL
                     TSAGALRKFDLVTANPMWNQDFSQTVYENDPYNRFVFGFPPSNSADWGWVQHMFASLN
                     DAGKMAVVLDTGAVSRGSGNAGSNRERDIRKEFVERDLVEAVILLPENLFYNTTAPGI
                     ILVINKAKPADRKEHLLLINAAHLFEKGRPKNYLPDESISRIADIYLQWKEEEGISKI
                     ITNQEAARNDYNLSPSRYVAQNGEDDTLPPEDAVIQLREAEEERQKADEKLKAILQEM
                     GLWKN"
     gene            18230..18823
                     /locus_tag="PLZ54_01565"
     CDS             18230..18823
                     /locus_tag="PLZ54_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004343129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ65660.1"
                     /translation="MTYNPNNHHRRSIRLHGYDYSQPGIYFITLCTYNRECLFGEILN
                     GEMRLNEFGKIANQCWLEIPNHFPQTQLDEYIIMPDHIHGIIVLNDFVGVQNVGTKNV
                     GTKIVGAKNFSPLPMPTPEPTREQNITPFRSPSKTIGSIIRGFKIGVTKWFRQNTDIY
                     VVWQRNFYEHIIRNEDELNRIRQYIIDNPKKWKTDEN"
     gene            18922..20106
                     /locus_tag="PLZ54_01570"
     CDS             18922..20106
                     /locus_tag="PLZ54_01570"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOJ65661.1"
                     /translation="MIMNSINDEILLPQGYKMTELGPLPEEWEVVKLGEVCKVDWGNT
                     SLTKKLYTANGYPAFSAAGNDGFLDFYEHDGEAVILSAIGARSGKCFYASGKWTAIKN
                     TIVIKSNNETKINLLFLYFFVNNENYWHKSGSGQPFISIGVAQKQKLPLPPLPEQQKM
                     AAVLSAVQEAKEKTRAVIDATKALKKSMMKHLFTYGPVSPEEAENVPLKETEIGKVPE
                     EWEVVKLGELAEVKYGKACPKEQGNVPVIGSGGIYFWTNNAIVEFPTIIIGRKGTAGK
                     VWLSLQPCYPSDTTFYLKWLEDKINIKFIFEYLNINQLSGEHAKTTLPSLQRHELEKF
                     LLPLPPLPVQQKIASILSAIDEKIQAEEAKKKALEDLFRSLLSHLMSGKIRVKDLGIG
                     NC"
     gene            20186..23128
                     /locus_tag="PLZ54_01575"
     CDS             20186..23128
                     /locus_tag="PLZ54_01575"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HOJ65662.1"
                     /translation="MAKLGGERYSVQQPIIQYVQEPSAAYVSQDGLTMFLKLGWEYVK
                     PDDALRLKGGEAGMVFREIFIRQLQRLNPDFLDHLMAEELIKKIETVPANIEGNFLTW
                     EYLKGLRTVFVPTEKRERNITFIDAENMDRNTFHVTDEFVFSNGIKTIRPDIVFLING
                     IPVLFIETKAAHKIEGMSEALEQIKRYHRDGPELLAVLQVYVLTDIVKYLYSATWNTS
                     EKLLFNWKNEAGGDFEQLVKTFFDRKNVIKLLTDFILFTRQDDELRKIVLRPHQMKAI
                     DKIVKRAEDPQKQRGLIWHTQGSGKTFTMIVAAQKIIENPLFENPTVIMLVDRNELES
                     QLFGNLAAIGMSNVVADSKEHLQKLLSGDRRGLIVTMIHKFEGMPKNINLRKNIFVLV
                     DEAHRTTGGKLGNYLMGAIPNATYIGFTGTPIDKTQYGEGTFITFGKDDPENAYLDKY
                     SIAESIADGTTVPLYYTLAPNELRVEKEVLEKEFLELKAAEGMSDVEELNKVLERAVT
                     LRNMLKNKERVEKVAQYVANHFLNTVEPMGYKAFLVGVDREACALYKEELDKYLPADY
                     SQVVYSSFYNDPEELAKYHLSDIEEKRIRKAFRKPDELPKILIVTEKLLTGFDAPILY
                     CMYLDKPMRDHVLLQAIARVNRPYEDEEGRKKVSGFVLDFVGIFDHLEKALAFDSQDI
                     AGIVRDVEMLKERFKEEMEKARKNYLTLIGGKKQDKAVETLLNYFLDEEIRNEYYRFF
                     KELSTVYEIISPDAFLRDYLEDYETLSRMYQILRENYDRGIDINKDFTRKTIELVKKH
                     TKSGEIQPTIDIFEINEETLEEIEKSKTSDIEKVFNLIKSIERTVAQEGNTAPYLKSI
                     AEEAEMLAKLFQERQKTTQETLEELKKIIEEIVTAKKEQVEKNMTSEIFSIYWILDRS
                     GFENATSMANEMEKVFEEYPHWQKSNKHGIKIRQKLYEVLVHSGITDSKKISETAQQI
                     MKLLNVKI"
     gene            23130..23411
                     /locus_tag="PLZ54_01580"
     CDS             23130..23411
                     /locus_tag="PLZ54_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOJ65663.1"
                     /translation="MTTHEELKIEVMTLAEEVGVKPNAIHIRAMKRKWASCSSKGRLT
                     FNETLLDEPKEVQYKVILHELLHLKYPNHGKMFNILLKSYLEKISPDKN"
     gene            complement(23773..24378)
                     /locus_tag="PLZ54_01585"
     CDS             complement(23773..24378)
                     /locus_tag="PLZ54_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ65664.1"
                     /translation="MSLGYDIFEGNIYEGHTLIPFLEEMSCKFHLNKPVVIADAGLLS
                     KENIKALESNEYEYILEARVKNQNERIKEKILAIKFTDGMTVSIKKSDNIRWIVNYST
                     ARAKKDCYDRKQGLDRIEKQIKSGKLTKSNINNRGYNKYLKLSGKISVEIDYETHMYC
                     IEEVMKNYKNLWQIEMAFRISKTDVCIRPIYHRKRNRTQRN"
     gene            complement(24396..24728)
                     /locus_tag="PLZ54_01590"
     CDS             complement(24396..24728)
                     /locus_tag="PLZ54_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65665.1"
                     /translation="MKTIGCATTQHEIERLEQLAGEEMDRLSGDQLKLFGYESDEMIE
                     QAFSLLDNSSIHTVGPELIFGKIYDFIGFNSIQEELFRHLVIARLAFPLSKLKTVDYL
                     RLPIPLKF"
     gene            complement(24968..26143)
                     /locus_tag="PLZ54_01595"
     CDS             complement(24968..26143)
                     /locus_tag="PLZ54_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HOJ65666.1"
                     /translation="MKKLFLLIFTTFICLNGIAQKFDGLANTPPMGWNSWNKFNCDVN
                     EELIMKMADAMISSGMKDAGYEYIVVDDCWQVSRDENGNIQHDTQRFPHGIKYLADYI
                     HSKGLKFGIYSDAGIKTCQGRPGSRGYEFQDAKTYASWGVDYLKYDWCNTGTQDAKAS
                     YSIMRDALYAAGRPIVFSICEWGKSRPWEWAQEVGHLWRTTGDIVDRWDAMIDIVDKQ
                     RDLGPYSGPNHWNDPDMLEVGNGGMTIEEYKTHFSLWCMMAAPLMAGNDLSDMSKETL
                     EILTNKELIAIDQDKLGKQAFCFRDNGDYEIWIKKLSNDEKAVCLLNRSDEIKEVMVD
                     FAFLLKSNDDYWTQDPYQLQNYIVRDLWEHKEIKTDKYPTFVKIPPHGVKVYKYIKK"
     gene            complement(26328..27779)
                     /locus_tag="PLZ54_01600"
     CDS             complement(26328..27779)
                     /locus_tag="PLZ54_01600"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOJ65667.1"
                     /translation="MNNRSSALDALRGLAIIGMVLSGTVAPTLPAWMYHAQVGPRSNF
                     NFDPTIFGITWVDLVFPFFLFAMGAAFPLALGRKLDKGATVTSLFPAILKRVFLLVLF
                     AIAIYHCSPYRLGGGWNFALAILAFGLFFISFVRFPNVSEKKNTWLNWLGIAFLMGLI
                     AFNVSSQPEVFSKGFKLSHNDIIILVLANMALFGSLAWILTRTNLLLRLGILAFLFAL
                     RLTSGVDGSWNQVLWNFNPMQLLPDSITTIVYNPGGWLYRMDFLKYLFIVIPGTIAGD
                     LLVRRFTDKDNEKSILPSKQKMGASLLLMVAFVLSNLICLFGRFLLLNLILNIAFSIA
                     GYILMRKPTHSLSRLYFSLYKWGIFWLMLGTAFEAFEGGIRKDSATMSYFFVTSGLAI
                     FTLLFFSFVIDYFENKKLFRYISECGQNPMVAYVAGTFFVVPILVFTGLMPHINTLHE
                     ITPWFGILKGVIITSGMMAVTVFTVRRKWFWKT"
     gene            complement(27802..29817)
                     /locus_tag="PLZ54_01605"
     CDS             complement(27802..29817)
                     /locus_tag="PLZ54_01605"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HOJ65668.1"
                     /translation="MKKITLLLISFYIAFNADAINAVVYDGSGQLSEDKLIALTLAGI
                     VNRDAPRLYLRNVYETWSFNQTDELWENEYKTVGGVQFTVITNINALVQYFKSYIKGA
                     ITYDATLTYSNFSGQSFRWQAEAAAMLGGLTDCIPVPSTNTIIDINKPTSVDLPDYFN
                     GQQTVPVSAKLELTSHSWNNTALTQEQRYFAWLDWALDNLLPRCNTSKFYLREITDWA
                     VNQRMFQLNLAGTEDLRFTSLSDEKAAKIERVMNYLKTKRPNEIFHVYGWMRPEPLVQ
                     WISAWGGSFHETLLSNLSWHHVFPVDANFSYQRPADINPELVLLEDKYYVLFISSEGD
                     AGNWNIGFQGGAWASTTRGQVPVAWGFNLQMFEEFPFVAQYYYRTATANDGFMAVSTP
                     LGYAYPDVFPTSYLPDAKAKTIALMDKFNVNSVYAYKHYNGAGSSQYRGITISNNFNF
                     SNLGLFAEQTNAQMTMLFDPALMTQKAYTNYGGLLYNHVNDDTFYADFSNLTTVRDRI
                     VSKLSGKTKPRFLMAGYQRFRFDNATLATNDVTLPRLKTLMDDIKNNTSIGADVEFVT
                     PEKFTYLLRKSLGLSTTVPAVENNGQKLLVFSDQNNRLQLNLQLESAQLVQIRVFDLA
                     GKLILSENWNMTMSNDFKTISVAQKGIFILNIKGQNVNLTSKIVNGK"
     gene            complement(29890..31686)
                     /locus_tag="PLZ54_01610"
     CDS             complement(29890..31686)
                     /locus_tag="PLZ54_01610"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HOJ65669.1"
                     /translation="MFSTFYSCNEPDLSNPKAPFPAPTELVVEKITTTSVKISWSDNS
                     NGEDGFTIERWVTGTSVPVKATVGANTTEWIDEGLQEATYLYKVYGFCKQRVSDTVSV
                     YYQHVPVSMPTNFTVVAAHNTVNLTWDALTENIDGYKIERKIGTEIYQAWKTLDANTT
                     SVTDSDPAVGLNTYKLYAYAGSATSVGVERSLMILKTPIITIASPIVSYLKISAYFTL
                     TSDGGEPCNAGLCWSKSPNPTITDSKSVWHVKVKSGETFFGNAVNLDKNTNYYIRAYA
                     TNSKGTTYSPEIQATLSPELPAIALSWTPITSINAELPTEINVYSTTSSLNGRPFSAW
                     YAIADMSTGIIELKTTLSTSPQKPSQFIAAATNETTYVMTNAGYFGYSGSTVNSYSLA
                     VDRTSKKSDNISTLTRGSHSYAVTRGAFGVSQNQTPSIKWAVGNFAYDIPSPNVEGET
                     PQPSLSASFPAQSQNWNPYSAIGGAPVLLKDGKIVFDFTTTTSGKYMTNYELLQTDIF
                     STTARPPRTVIGSTADNKIVLFVCDGRQTHSDGATLIELEQIMKGIGCVNALNLDGGG
                     SSAIIAGGKLLNKPSDGTERGVATVVSFVKKK"
     gene            complement(31756..33408)
                     /locus_tag="PLZ54_01615"
     CDS             complement(31756..33408)
                     /locus_tag="PLZ54_01615"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ65670.1"
                     /translation="MKKFFLFFTCILLLSCKQSLEGGNEIISFKFQEISQANEAYISN
                     DTVLIMVPTNTDLTSLTPVIEVSVKATINPASGVKQDFSKPVFYTVTSELGTKKSYIV
                     LVYSQPTFRVNNVKLPDNFVTGTINGDNITFTVPFGTNVSAMKLTYLLADNGLTSNIA
                     SGSTVDLSQPFLWKLSIGNLSKTYTITANIQPQETGVRAVWVTNVASSVLFSESALTD
                     MVDKLAELNFNTICVCTYNKSQTLFPSQTLATVLGTTPAQTVFSGCSWGDVLEKLIEK
                     AHAKNIKVIAWFEYGFASHYSGQDSPISDKHPDWVSKQQDGTQTVKNSFYWLNGFHHE
                     VQQFMTDMIVECVTKYPELDGIQGDDRLPALPAHAGYDDYTKQLYKTETGRDVPSNPT
                     ETIFFNWKVNKMSDYAVSLYNAVKAARSTCLVTWAPSTRGWSRDNYLQDWPEWLRRGV
                     ADWISPQLYRNESQGVDSYTTLVYSDVQNVFNTADLRKKYVPGMLVRNGNTIVSDEFL
                     AQLIQYNRTKGVYGESTWFYEAIPSKQKVYKALYPGKAIFPF"
     gene            complement(33528..34958)
                     /locus_tag="PLZ54_01620"
     CDS             complement(33528..34958)
                     /locus_tag="PLZ54_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ65671.1"
                     /translation="MLPDKIKEKISEIGTDFNENKLESINILKGFKALKLTEGFSEFK
                     HLKRSGYSFQLVLSSMIYAVTPGRKTAVSSLPLLKEKGITMGKNILYRLKNNAGINWR
                     KLLWQTACKFIGITGQEKEVSCKVRCLIFDDTLLEKTGKKMEKIGKVYDHVTNTFKLG
                     YKLLLGLYWDGKSMIPLNFSLSREKGKRAEKPYGMSRKELRHQYSKKRLKELSGRERE
                     KELDKSKIEMMLEIFYRAVFYHIPLDYVLCDSWFTCQALITAVGSKHIHLIGMYRIVK
                     AKFLYQGKQMNYREIQSSIDKITRCRKLKLHYKRADVMLDGIAVTLFFTRQGKRGKWK
                     VFLTTDTKLSFVQLMEIYQIRWSIEVFFKETKQLLNLGGCQSNDFDAQIADTTISMIA
                     YILLSFRYRYEHYESMGELFRVMNTECLQQTLDERLWGLFLELLQEICEAFEKDIDEL
                     FELIMNCPKTAELVSKMLTPPLREAV"
     gene            complement(35266..36732)
                     /locus_tag="PLZ54_01625"
     CDS             complement(35266..36732)
                     /locus_tag="PLZ54_01625"
                     /inference="COORDINATES: protein motif:HMM:NF027725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5018 domain-containing protein"
                     /protein_id="HOJ65672.1"
                     /translation="MKTNRLFNKIAIVLFVALSLASCKNEPEVVLKTDSEIKSFALDL
                     SSLDALADQTIKASISVEIVGEEIQINLPFGLKPDTINRLLNSNAATLTVSEGATVSP
                     ASGTPVKFNSATPVYYTVTAEDGVSKTAYKVRFFETAQKGYGVISEYWRKNGTAFTDK
                     FTDNNEKTFALTPTHIVQQIGGLYGGTLKLVKVSLLDGSETGYAKLVVAPGDTIKQIR
                     AMAGDRKGSFIGCNLAAPGGDFKVWCWDNVDAAPRELIKWNVSDLTPVGTPPSWAGNH
                     VGRRISVIGDLKTQAYIYTYANYTLDILRWKVTDGVAASSPEFIHYTPGGSMSRFELW
                     ASIVPLGATATSDILVNAACAGSEVGVMNGITKQTYMFATAVATPKGIATYLEFNNVK
                     YYIPLNVEAYATPHSVNARLYDYTTTTNSVEMSNNWWLTPQPEKIEADGTLKNGDATG
                     CIDAILDADGLGFKVVALGTGCGIVVTKFSKKKELTFN"
     gene            complement(36793..38499)
                     /locus_tag="PLZ54_01630"
     CDS             complement(36793..38499)
                     /locus_tag="PLZ54_01630"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOJ65673.1"
                     /translation="MKNTIFILLVAVVFSACTDRFAEINTNPNASTTIDPAYLFTTSQ
                     RRGAMDDRVYYYTRGMACFHTQMYANTNYTKEHCTDRYQFNAAWNGTLWSAAYAGDAS
                     KTYLSFVAEAEKIAKQKGWENKEAICNIWKSYLFLQLTDLFGDIPYSEAFNDLIDYPK
                     YDKQEDIYIGIYNSLKTSVQKIDATKGNGMGSADLFYNGDYSSWKRFGNSVRLRMAIR
                     ISKVNPTLAKTWFDETLADAGGLMSNNLQSCRLKTDPAGPQGIHSDNPIKVMANAFDW
                     YRISEFMVKTLNELDDPRIFNFAEPTKNYLNALKQICKDIEQNSTIYNSLSYAEQAKV
                     SYAAAEGGSFINIFAQGRYFTDEIEAIVARVLPSRSSEISRSIKYRGLRNGQSIESLT
                     TLQSKLGEYSDLSQYLRQDNYPTNIFVYPEICFLLAEGNLNGWTTPKTAEEHYNDGVR
                     AAMQLYEVPDSEINKYLAGAAKFKSNGTKAEKLEQIILQKYLGNYTDGFEAYADLRRT
                     GYPKILPIEYTSSVPDEMELPRRWKYPSDEHLLNKANVTEAANRMGGDYQLSRMWWDV
                     NN"
     gene            complement(38527..>40230)
                     /locus_tag="PLZ54_01635"
     CDS             complement(38527..>40230)
                     /locus_tag="PLZ54_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65674.1"
                     /translation="AKRTAYENNETRSKYAQNWGKSFENNFDLLLSYKNNFMDNKFSV
                     VATGGGNFMHTWGNSIGFYALALSLDDLYTVGNAKNVTSSQGFSQKQVQSLYGTVSLG
                     YNSYLFLDLTAREDWNSSLYSNINPSASVLNYFYPSASLSALFTDAFPAIKSNFLSYL
                     KVRLAYARVGNGTSPYRTSYTYSIGPGHLGQQYAYKPNVKPFVTLKPEMMNSSEVGLE
                     ANFFQNLLKLDATYYLNRTSNQIIAQRIPSPSGYTTKLMNAGLVQNQGVELQLTTNIF
                     NKKDFHWDVFVNFSRNRDRIVELLDPNKKDRRIIASAGTLSIHAKAGGRYGEIYGTKY
                     LRNDKGERIIGSNGLPMLAEDSIGSRTDNYLGNIQNDFLGGLGTNLSHKNFNFSALID
                     VRMGGKLYSFTEKITTEYGNAPWTTTGREEWAASEIARIAAGKSPVEWLASRNQRGYL
                     PQGVIQLPDGSYTPNRNLYVSPQSYWINAAGVDEEFLHDASFVKFKELRLGYKLPKKW
                     LKPLTINNLEIAAVGRNLFYIYKAAPKGLDPEASFNGMGFEYSSIPSTREWGASMSVS
                     F"
BASE COUNT        12524 a         7987 c         7494 g        12225 t
ORIGIN      
        1 agtaacgttg ctcccataaa catcatcaaa ctgagcgtcg gaaaccaatg aaaattgaag
       61 tatttcatca ttggttgcgc ttttagcagc aaaatgaaac ggaaaataga cggcattgaa
      121 cccactcaga aaaacaaatt ttccctgccc gtaatcggaa ttggtgtgtg aaaaaacgga
      181 gtcgaggctt gctaccggag gaaataaaaa atcaccggca ctgtcaacgg tttttgtcaa
      241 acgaaatgaa accagattat ttgtataggc gttcaatccg agttgaaaaa agacagtatc
      301 acctacctga atactatcca aaagataaac cccagattca tcactagttt taatgtttaa
      361 cgtatcgcca tgttgggtgt agggataagc taaaaattga atagcaggag tgtaattgga
      421 atcgccatca atattgcacc cggttaataa aacaaccgcc atgaacgata gaagaacaaa
      481 ctgttttttc ataatatttt gaaatttgaa gtgaatgaaa ggaagcaaca aaaaacatac
      541 ccatcaactt gtaacatcaa atccaacttt gtcggggtga caagactcga acctgcgacc
      601 tctacgtccc gaacgtagcg cgctaccaac tgcgccacac cccgccttga ttattcggct
      661 gcaaatttac atcatttttt gatatttcga gcatgaaaat aagctaaaat tattcacaga
      721 tggatatccg cataatttta accattattt atgtgtttgt atattagtta tttaacaatt
      781 ttttgaaact ataaattagg agaataagcg ttgaaattac tacctttgtg aaaaataatt
      841 aaattcatgt ttaaagaggc tattcaaaat tatctgcaac gacacgacta caaaagtttt
      901 tatcccaaag ccgtattttt cgatatggat ggagtgctga tcgactccat gaagaaccat
      961 tccagagcat ggatgcaagc catgaaagaa tcaaacctgc cttttaccga agaagatgcc
     1021 tacatgcacg aaggttgtat tggtgctacc accatcaacg atacttttat gaagcatctg
     1081 aacagggaag ctactgaaga agaacaaaaa caaatttatt cagcaaaatc aagaatcttt
     1141 gaatcgcttg agcccatcga aaaaatgcct tttgcttatg aattgctcaa aaagttgaaa
     1201 tcggaagaaa tgaaaattta tgttgttacc ggctcgggac aacccaaatt aatcgacagt
     1261 ctgaatacgc ttttccccga aattttcaca aaagaaacca tgataacggg tttcgacgta
     1321 aaagaaggca aaccatcacc cgaaccctat ttattggcgc tgaaaagatc cggatttcaa
     1381 ccctgggaaa cgatagttgt tgaaaatgca ccaatgggaa ttttatccgc ttcaaaagcc
     1441 ggcttgttta ctttagttgt caatacaggc ccgtttgacg atgatttcct ttatagctat
     1501 ggagctgatt tggtactcga cagcatggaa catttgtaca atgaatggga caatttccgt
     1561 aaatcgtgga tttgaaatga aaaagacagg tttggaactt ttcctttctc ccgtttttct
     1621 ttccctgatt ttattctttt cttcaaccgt tatttttgcc ggagaaactg attctgcttc
     1681 ctttcatccc caaatagaag gaacgatcag ggcgaagtac gaatacaaca ccagtttgaa
     1741 cgcttcccgt tttcaggtac gtaacgctcg ttttagcgtc aacggaaatt ttaattccct
     1801 cgtttcctac aaaacggaaa ttgatctttc cgacgaaggt caaaccaaaa tgctggatgc
     1861 ctatattcga ctgcaacctc aaaaatggtt cactttcacc atcggacagc aaaaaattcc
     1921 tttcagcacc gataatcttc gtcctcctca tttgctgtat tttgccaatc gatcgtttat
     1981 cgggaaacaa ctcaccgcac tcagagatgt gggcactaca ttaagttttg taaataataa
     2041 aactttacct tttgaattta taaccggttt gtataatggc gagggtcttt ataatcagaa
     2101 aatttggcaa acttcattca gttatgcttt tagattggtt ttacatcctt gcgaaaatct
     2161 acatttttca ttaaatcata attctattcg tcctgaagaa atgcggatga atctatatga
     2221 tttgggaggt attttacagc tcaaaaattg gcatctcgaa accgaattgt tctataaaac
     2281 ctatcaacag aattttttcc ctaccaccaa aggttttttt gttttttcga gttatgatat
     2341 atatctacat aataaagaaa gtattaaaat tattcgtcca cttatacgat acgacagtat
     2401 gacggacaat aacaaaggtt acaaatcgga aaacggaagt tatgaatcag acgacatggc
     2461 aagatcaaga attacaggcg gaataacttt gtgtcttgac aaaccttttt taaatgatat
     2521 tcgattaaac tatgaacagt atttttataa aaaaggcatt caaaacaatg acaataaatt
     2581 tgtggtagaa tttgtacttc gtttttaata aaaaagcatt aaatttgcaa tacgtttttt
     2641 attttctcca aaaaaacaaa aatcaggaag aaaatcaatt atgcaagtaa taaaatatcc
     2701 ggccagagaa ttttggtcaa cccttctctc ccgccccgcc attgatagaa aaagtttatt
     2761 tgaaaaagtt ggaagcattg tttcagaggt gaagcaaaac ggagataaag cagtcaaaaa
     2821 atatacctta cagttcgaca aagttggcat tgaagaactt gaagtgagcc aagaagaaat
     2881 tgaagaagca tccttgcaga ttccaaacca gttaaaggaa gccatagaaa tgtgcagacg
     2941 aaatatttgg aaatttcatt ctgcacaaca acacgattta ccggaaattc agactacacc
     3001 gggagtggtt tgctggcaaa aagccatgcc catagaaaaa gtggggttat acgttcccgg
     3061 aggaacggct cctttgtttt cgaccgtttt aatgttgggc gttcccgcac aaattgccga
     3121 atgcaaggaa attgttcttt gtacacctcc cgataaagaa ggtaaaattc atccggctgt
     3181 actttatgct gcaaaagttg caggcataca cagaattttc aaaattggag gaatacaagc
     3241 aattgcagct atggcttacg gaacagaaac tgtaccgaaa gtgtataaaa tttttggacc
     3301 gggaaaccag tatgtaactg ctgccaaaca attgattgcg ttggatggta ttgcaattga
     3361 ccttccggca ggtccatcgg aagttatggt gatagccgac gaaagcgcac gacccggttt
     3421 tctggctgcc gatttgcttt cacaagccga acacagtgtc gacagccaat ctattctcat
     3481 tactgttagc gaaaatcttg tagaaccggt tcttttggaa attgaggaac agctcaaccg
     3541 cattccaaaa aaagaaatcg ctctcaaagc tttggaaaat ggtaaaataa ttgtgctgca
     3601 atcgttggaa gaagcagtag caatggcgaa cgaatatgca cccgaacatc tgattatctc
     3661 cactcgttac agtttgggag ttgcatccaa tatcagcaat gctggctcgg tatttctcgg
     3721 acactatacg cccgaaagtg ccggagatta tgcttcgggt accaatcaca ccctgcccac
     3781 caacggttat gcaaaagcct acagtggagt taatttggac agttttatgc ggaaagttac
     3841 ttttcagcaa atttctcgcg aaggactttc caatttgagt aatgccatta ttttgatggc
     3901 cgaaaatgaa ggtctttatg cccacagtaa cgccgttaaa gtgcgattgg ataaagaaga
     3961 atcagaggaa aaacccgaaa accggctttg ttaatacatg cattacactt ttcttcgttt
     4021 gaacaacaaa tttagaatca aaaaaatcac atgataaaat tagattagaa tgaaaataaa
     4081 tgaattagta agagagaaca ttcgtaatat gcagccatat tcgtgtgctc gcagcgaatt
     4141 taagggcgaa gcttccgtgt atcttgatgc caacgaaaat ccgtataatt cacctttcaa
     4201 tcgctatcct gacccgctgc agactgagct gaaaaaagaa atttcaaaaa ttaagaaagt
     4261 tcccgttgaa aatattttct tgggtaatgg cagcgatgaa gcaattgatc tcttgtacag
     4321 agctttttgt gaacccaaaa ttcacaatgt ggttgccatt gaacccagct atggtatgta
     4381 taaggtttgt gctgaaataa ataatgttga atacagaccg gtcttgcttg atgaaaattt
     4441 tgatgtggaa gcttataaat taatggatgc cagcaatgac aaaacaaaaa ttatatggct
     4501 atgttcaccc aacaatccta ccggcaacag tttgaatttt tcggaaatca acaaacttct
     4561 ttcatggttc agaggaattg tggtcatcga cgaggcttat atagattttt ctacaaaaaa
     4621 aagtttactg aattacctga ctcttttccc gcaattgata gtattgcaaa ccatgtccaa
     4681 agcgtgggga aatgcaggaa tacgtttggg aatggctttt gccgatccgg aaataatcgg
     4741 gatactgaat aaaatcaagt atccttacaa catcaattat cttacccagc aacatgcttt
     4801 acaagcgttg caaaatccta tgcaggtaga aaaatgggta gcattaattt tggaggaaaa
     4861 agaaaaactg ataaaagaac ttcagaaatt aaaattggta gaaaaaattt atccttctga
     4921 tgcaaacttt attttggtaa aagttgaaaa tgccaatgca atttatcgat ttctcattca
     4981 aaacgggatt gtcgtacgca acagaagtaa tatgcctttg tgtaacaact gcatacgcat
     5041 tactgtagga accccagaag aaaacaaagc attaatcgat acactgaaaa aatttcacgt
     5101 gtaaaatttt actctccatt acgaaaaaag cgcttgaaag gaatcctttc aggcgctttt
     5161 ttgtagcggg aacgagagtt gaactcgtga cctccgggtt atgaatccga cgctctaacc
     5221 aactgagcta tcccgccttt ttgtttttgc ggtgcaaaga tagctttttt taataattcc
     5281 gacaaccctt tgcaataaaa atctattttt tcaatcttat aattcggtag ccggttcacc
     5341 ctttaacatt actaaacaag ctattttaac aatgaaactc aagcaaaaat attgttcccg
     5401 aaccatgata cagttattcc aagttataca ttctatttat ctcattatca gcaataaata
     5461 gataagcata gaaaactatt ttgcaaattt cgggttgaaa taaaaaaaga attgatattc
     5521 aatcaatgcg gttttttcgt gggtattgat ttattgtaac cagcagatga ttaaatttcc
     5581 cattgttcac aagcatcaat gagttcatca aacgatttta cggaagtttg tttttttatt
     5641 tcattgattt gattttccat agccatttca taagtttctt cctccacatc acgaataatt
     5701 cccatagcta tgggcatttc tggaccttcc atcaatgcca gtttggtatg taaaaaagta
     5761 tctttgcact tggcatcatg aaccagaata tccttatctg taattccatt ttcaccaatc
     5821 gttacgactt ttagttctga tttttccaaa atcaatcctt tatctttatt ttttccgaaa
     5881 atcatgggct taccatgttc caacacgaca attctatcat ctctggtttc tttttcggcc
     5941 aaccatccat gcgcaccatc attgaaaatc acgcaattta ccaaacattc tactacagaa
     6001 gttcctttat gaagggcagc agccttcatg accatttgag aagttttcaa atcgacatcc
     6061 aataatcttc caaaaaatgt tccaccggct ccaaaagtca gttcagccgt acgaaaaggt
     6121 cgctcaactg tgccataagg agaagatttg gtaacaaaac cttttttggt agtaggagaa
     6181 tattgtcctt tggtaaggcc gtaaatctga ttattaaaaa gtagaacatt aatgtccaca
     6241 tttctcctta cgctatgaat aaaatgattt cctccaatag ccaagcaatc accatcaccg
     6301 gtaacttgcc aaaccgtaag cgcaggattg gcaactttaa caccggttgc tacacaagcg
     6361 cctcttccgt gaatggtatg aaatccataa gtattggtgt aataagggag acgggaagaa
     6421 caaccaattc ccgaaataat gaccgtttcg tgtggtgaaa cacccaactc tgccattgtc
     6481 ttgaccagag cattgaaaat ggcataatct ccacaaccgg gacaccagcg gggttgctgg
     6541 tctgttttaa agtcggcagg catatatttt gtctcttcca taatttctat gatttttttg
     6601 gatgcatgaa ataattatag cagcgaaatg aaatgatttt ccaattcttc cgttttaaaa
     6661 ggctggcctt gtattttgtt gaattgggta aaggtttttc cgggaatttt agatcgcaag
     6721 taggaagcaa attgaccggt attcagttcg caaacaacta ttttttcgta ttttccaata
     6781 acattttcag tattggcagg aagcgggaaa agataggtaa aatgagccaa cgctatttta
     6841 tatcctttgt tgtttaagtt gtccacagca cttttcaaat gcccgtaagt tccaccccaa
     6901 ccaactacca acaaatcggc atctgtattt ccttcgattt cgagttctgg aattacattt
     6961 acgatattgt caaccttctg ttttctggtt aaaaccattt tttcatgatt ttcgggatcg
     7021 gttgaaagat ttccggttgt ataatctttt tccaagcctc cgttgcaatg ttgaaaacct
     7081 ttcattcccg gaatactcca ataacgaatc ttggtatctt cgtttcgagt ggtaaccttg
     7141 tagttttcca tattcgatgg aacaaaatga ggatgaattt ctggtaagtc ggcaagtttt
     7201 ggcaagcgcc aaagagaact tccatttcca ataaaagcat ccgtcagcag aataacgggc
     7261 gtcatatttt ccaatgcaat tttgctggcc ataaaagcat aatgaaagca cgagtcggga
     7321 gttggagctg ccaaaacagc aagcggactt tctccgctgc gtccatataa tgcctgcaac
     7381 aaatcaccct gttcggtttt agtaggcata cctgttgatg gaccactgcg catgacatct
     7441 acaattacca aaggcaattc ggccattacc gccaacccga ttccttctgt tttcagtgac
     7501 ataccaggcc cggaagtact tgttgcagcc aagcagccag caaaagaagc ccctattgcc
     7561 attgtaatac ctgcaatttc atcttcggct tgtacggttt taacaccaat acctttttgc
     7621 aaagccaatt cttgcattat gtctgtggcc ggtgtaatgg gataacttcc caaaaaaagc
     7681 tgaagtccgg ctttttcggc tgcagcaatc aatccccaag ccgtagcttt gttgccgtta
     7741 atacttgtgt atttgccctt tggcatttcg tgcgattttt ctatcacaaa attggatacg
     7801 gtcagatgaa gattttcgcc ataattgtat ccatctgtaa gagccttaat atttgcttga
     7861 agaatttgcg ttttttttct gaatttattt tccagaaaat gaatagcctt atccagcggt
     7921 cggttgaaaa gccagcaaac cagccctaaa gcaagcatgt tcttgctgcg caataccgtt
     7981 ttgttatcaa aaccaaagtc ttcgagactg cttttggtaa gtgttgtcag tggaacagga
     8041 ataagctgaa tggtgtctga aagagaaagc tcttcaaaag gattatcagt ggaaaaattg
     8101 gcttttttta gatcagatgg ctgaaaagaa tccaaatcaa aaataacaat gctgcctttt
     8161 ttgagatttt ttgcgttcac tttcagcgca gccggattaa gtgtcaccaa gacgtctatt
     8221 tcgtctcccg gagtgtaaat tttgttggcc ccaacatgaa cctgaaatcc ggaaactcca
     8281 cccagagtgc cttgaggagc tctaatttcg gaaggaaaat cagtaaaggt agatatttcg
     8341 tttcccaatt ctgccgaaag ttgagcaaac aaatttcctg tcaactgcat gccatcaccc
     8401 gaatcgcctg aaaatcgtat aacgatattg tccaattgaa tggtttgtgc tttattcatt
     8461 tttgaaaata gtgaatgaat taaataatat cttacaaaaa cttttttaac tatgtgtatt
     8521 taaaaatcgg cattttttgg cgtgcgtgga aatggaatca catcacgaat attggacatg
     8581 ccggttacaa aaagaagaaa tctttcaaat cccaatccaa atcccgaatg cggcgcattg
     8641 ccaaattgac gtgtttcgag ataccaccaa atatctttat cgggaacttc catttccttt
     8701 gctctggctt tgagtttttc ataatcgtct tcccgttgcg atccaccaat gatttcacca
     8761 attttcggga ataaaacatc catagcgcga acggtttttc catcgtcgtt cagcttcatg
     8821 taaaatgctt tgatattttt tggataatcg gtgagaataa ccggtttttt gaaatgtttt
     8881 tcaacgagat atctttcgtg ttccgattgt aaatcaattc cccaacctac cggaaattca
     8941 aatttttgtc cttttgccat ggcgtcttcc agaattttta ttccttcaga gtaagttagc
     9001 cgaacaaaat cattttctat gacaaatttc agacggtcaa tcaattcggg atcataacga
     9061 ttgttgagaa attccaaatc atcactacac ttttccaaca cccaacgcac acagtatttt
     9121 acaaagtctt cagccaattg catgttttct tccagttcat tgaatgccac ttctggttcg
     9181 atcatccaaa attcggacaa atgtcgggga gtattggaat tttcggcgcg gaaagtgggg
     9241 ccaaaggtat aaatggctcc caaagccaaa gcccccaatt cgccttccaa ctgaccggaa
     9301 actgtcaacc cggtttgttt gccaaaaaag tcattgctgt aatcgataca tccgttttca
     9361 gcttttggca gatcggaaag atttaatgtg gtaacctgaa acaattgtcc tgcaccttca
     9421 caatcagaac ctgtaataat aggtgtatga aaatagaaga atcctctttc atggaaaaaa
     9481 gtatgaatgg ccatagccat gtgatgccga atccggaaaa ctgctccaaa agtattggtt
     9541 cgtgggcgca aatgtgcaat ttcgcgaaga aattccattg tgtgttcctt tttctgcaaa
     9601 ggataggttt caggatcggc tgttccataa attacgatat cttctgcctg tatctcaacg
     9661 ctttgtcctt ttcccagcga ttgggccaat ttcccggtta cacctataca ggcaccggtg
     9721 gtgatcggtt ttaaaaaatc gattcccaat ttattgttgt ctgctacaat ttggatattg
     9781 tgtacggtag aaccgtcgtt taattcaata aagcttacat ttttatttcc tcttcgggtg
     9841 cgtacccatc ctttaacgca aatatcttca ccgaagtcga ttttttttaa agcatcaaca
     9901 atttttgttc ttttcataag ttcgatattt ttattttcct ctgaaatttt ttgttaacat
     9961 cggagaaatc aattctttgt cccttctttt accctttaac ttttttacaa agatgcaaaa
    10021 ttacaaattt caaacgaaaa accagccttg tcgacataaa aaaagcgtct gaaatactat
    10081 gatcaggcgc tttttaaatt atattattca aaaaatcaac gttttacggg ctctatattt
    10141 cccattttac tcatggcaca acggaaacct atatcgttgg aacattgatc ttgatcaaga
    10201 taacgacgag tggaaggatt taaccagtat attcggtctt tccacgaacc acctttgtaa
    10261 actctagttt tgtcggaaat ttgaggagcc aaaacatctg taggatcaat ttttttcata
    10321 ttggtaatgg ctgcaatatc ggaagaatca gcataaagct gaaaatcggt cgaaattttt
    10381 gaagtaagat ctccatcctt ataattacgc acatcatctc cttccaacac tgtagtggca
    10441 attctaccca aagagtcgat tttaaaaatt ttattgccca cttcatcttc tttttcaaca
    10501 ataggcgtca aatacatatt tcctctaaaa gaattgtatt cggcaacatc ttcaaaactg
    10561 gtagcgcggt aaacatcaag aacccattcg tttacattgc cggccatgtt gtaaagtcca
    10621 aaatcgttgg gataatatga attgacggga gccgtgatgg tagctctatc gttcaggcta
    10681 cctgccgttc ccatcatatc gccacgtcca cgaacaaaat tagccatcat ttgacccaat
    10741 tgtttgcgag atggattacg tacctggtgc ccattccatg ggtaaatttt tccttcagga
    10801 ataaatccat ctttgccggc tttcaaccca taggcagcat attcccattc ggcttcagtt
    10861 ggaagtcgaa tatcggaaac cagtaaaata ccgtccgaca tatccacttt tctgatttcg
    10921 ccggtcaggt ctttttttgg atttttccca accggaggtt gatagcttgc ttgtttaaga
    10981 tatttttgcg tattaaaaac aaaattgtta ataatgttat caaaattagt ctcatttttt
    11041 aatgaggcaa aatcgggtgg tgctattata cctgctttaa ccaatgccaa ttcgtttacc
    11101 cgatcggtac gccaactgca ataatccatt gcctgttccc aagtaacgcc cacaatggga
    11161 tattgatcga aagcaggatg tgtaaaataa tgttcaatgt aaggttcatt gtaagccaac
    11221 tcttcccgcc atactttgcc atttggatag gttttttcca ccaattttgg agctgttttt
    11281 ccaaaaacga gattcatcca gtgagtgtat tctcgccaat cgacatttct gatttcgtat
    11341 tgatccatgt agaatgaact cacggtaatt cgccttcttc ggctgtttcg aggagcagtc
    11401 accacttctc ccttttcgcc tattgtgaat gagcctcctt ctatttctac catacctgga
    11461 ggtacattgg ttttaaaatc ttttctgaca gcaaatccgg ctcccttttc atcattgtat
    11521 ttccaacccg ttacgcgtga gtatccttct ttgtttgatt gacccgcaca acctaaaaac
    11581 agaagagcta aacagaatgt cgaaacttta attaagactt ttactctcat ggtttttttt
    11641 gaataattgt tttttgaact atgtttaaca gaataaacga tttataaatt tataaatttt
    11701 gtcgtaatat taatttgttt tgaaaacttc tatgttctta tctgaatcta taaaacaaat
    11761 tatttgcaaa tatagtactt tttaaataat actttcgtta tgaagttcca aaaaaatatt
    11821 tttcctgaag ttatttgtct cgattctttt gttattaact gtttttttag aaaaaacgta
    11881 taattttcat ttagtttttt ctttttttaa agataaatca aaagttcgtc agctaaattt
    11941 cttatttttg caatttgtag ttgattccga atccacaatt tttaaaattc atgtttttaa
    12001 atttaaacgg ccgtttggtt gatgtttctt ctcctgttgt aatggggatt gtgaatgtta
    12061 ctcccgattc attctatacg ggtagtagat ttttgagcga gaaatctata ttaacttcag
    12121 ttgataatat tttaagtaac ggaggtacta tcgtagatgt aggagcatac tcaactcgcc
    12181 catctgccga gtcggtttcg gtagaagaag aattgaaacg gctgtcgaga gcattagaaa
    12241 tcatattgaa acaatttcca aatgttattt tatcggttga tacttttcga tcaaaagtgg
    12301 ccagatatgt aattgaaaat tttcatgtag ccatgatcaa cgatataggc ggtggtacac
    12361 ttgacgaatt gatgtttgaa acagtagccg acttgagagt tgcttatgtt ttaatgcaca
    12421 tgcggggtac tccgcaaaca atgcaaacat taacgcatta tgaaaataat gttgtttctg
    12481 aagttttttc gtttttacag aaaggtctgg ctcagcttca tctattgggt gtgaaagatg
    12541 tgatagttga tccgggtttt ggttttgcca aaactttgga acaaaattat gaattgttgc
    12601 ataaactgca ttatttaaaa gagttgaacg ccccgatatt agttggtttg tcaagaaaat
    12661 caatgattta caatttactt cgactttcac ccgaagaggc tttaaccgga acaattgcag
    12721 ccaatatgct atctttgata ggcggagcct ctattttgag agttcatgat gttaaagaag
    12781 ctttacaaac tatacggata tttgagcagt atcaaaactt ttcgactttt ataaatgatt
    12841 aaaaaaagaa tttatgtttt ttcagattga atttaaagat attgtcgata tttttcttgt
    12901 tgcttttttg ctttatgaaa tttacaaatt attgagaaat tcaggggcta taaatatttt
    12961 tatcggcata ttgacattta ttgtttgttg gttttttgtg tcgtatgttt ttaaaatgca
    13021 gttgcttggt gaaattatgg atacggtagt aaatgttgga gcatttgctt tgattgtcct
    13081 ttttcaagat gaaatccgac gtttttttac tcaaattggc actaaaagag aaaacagatt
    13141 tttatcgaaa atgaaaaaaa tttttggaga atcagaatac gataaaaagg aaacagattt
    13201 tgacttgttg caggttgtat tggcttgtcg caatatgtcg agaacaaata ccggatgttt
    13261 gattgttatt acaaggagga atgctttgga attttatgtt cagtcgggtg aattgattga
    13321 agcaaaaatc aattcaagat taatagaaag catttttttt aaaaatagtc cgcttcacga
    13381 tggggcgttg ataatttccc ggcacaccat aaaagctgcc ggctgcattc ttccggtttc
    13441 tcaaaaccaa tcgataccaa aaaggttagg gttgagacat agagcggcgc tgggcattac
    13501 cgaacatact gatgccgtag ctattgtagt ttccgaagaa acaggtcata tatcttgggc
    13561 agtcaatgga aatttgactc acaacgttaa acccgaagag ttggaatatt ttttaaatac
    13621 ccaacttcag ggaagcaaca aagtggagga agaaaaaata aaataaaccg atactttttc
    13681 cttagatttt tttagaaaaa gtatcggtaa attatttaat caaatatcat gttttcaggc
    13741 ctttcctgca cttcctagta cattcaccgt tttgtgcatg tacaattttt tcaattcatc
    13801 gcgtgcaggt cccagatatt ttcttgggtc aaattctgcc ggcttggtag caaaaacttc
    13861 acgaacagct gctgtcatgg ccagacgacc atcagaatca atattgattt tgcacactgc
    13921 agaagtagct gctctacgca actgatcttc gggaacacca atggcgtctt tcaatgcgcc
    13981 accatatttg ttgatttttt ccacatattc gtgaggtacg gaggatgcgc cgtgtaaaac
    14041 gatggggaaa cccggaattc gtttttctat ctcttccaat atatcaaagc gcaatggcgg
    14101 tggaatcaaa attccgtttt catcgcgtgt acactgttcg ggtttaaatt tggtagcccc
    14161 atgtgaggtc cctatggaaa tagccagtga atcgacattg gtgcgggtta caaaatcaac
    14221 tacctgatcc gggtcggtgt aggtatggtg ctcggcaaca acatcatctt caataccggc
    14281 taaaacaccc aattctcctt caacagttac atcaaactga tgggcatatt ccacaacttt
    14341 tttagttaaa gcaatatttt cttcgtaagg gagatgagaa ccatctatca ttaccgaaga
    14401 aaaacctgat tcaatacagc ttttgcagag ttcaaaagta tctccgtgat ccaaatgcag
    14461 cacaatggga atattgtaac ccagctcttt ggcatattca acagctcctt gtgccatata
    14521 gcgcaataat gtttggttgg catatttgcg tgcaccgctc gacacctgca aaattaccgg
    14581 cgattttgtc tctacacaag cagaaatgat agcctgcagc tgttccatgt tgttgaaatt
    14641 aaatgccggg atagcgtatt ttccttcaat tgctttttta aataactctc ttgaattgac
    14701 taatccaagt tcagtgtaat gtaccatatt ttttgaattt taaataatta aataatcaaa
    14761 ctttgcaaaa ttacgaattt ttcctgacaa atgaaaagaa aaatcttcac tttgtttttt
    14821 cattccaatc aattttctat caactaaaac agaattttct tgccaattgt ttgaaatagt
    14881 aaaataattt ttcttaaaaa ttttattaat caacctctta atttaatttt gcacattatc
    14941 aaacatttta aatttaaaga ttatgaaact ttggaaagag tttaaagaat ttgccatgcg
    15001 tggcagcgta atagatttgg caattggtgt aattattgga agtgcttttg gaaaaattgt
    15061 ttcttctctt gttgctgatg ttatcatgcc accaattggg ttgctcatca gtggtattaa
    15121 ttttacggat ttgaaattgg ttttaaaacc ggctgagatg gtaaacgatg ttcaaactcc
    15181 tgccgtaaca ttaaattatg ggaattttct gcaagttgct tttgatttcc tgattgtggc
    15241 ttttgtgatt tttcttttga taaaaggtat caacaatttc ttgaaaaaga aagaagaaaa
    15301 acctgtccct gaagcaacag cacaaccgag taaagaagaa attttgctga ccgaaattcg
    15361 agatatttta aaagagaaat aaaaaacaaa caaaagagat gcttggttta tgttgcataa
    15421 caaacctcag aacagagaca aaaacacaat ttcaattcct tttggtgcga ttaaaagttt
    15481 ctccgcagtt gtggagttcc gcataaggaa gtaaggactg caaccagatg gaatagatgt
    15541 caatcttttc ggaacgcaat gatttgcgca atgcgcctgt ccatatcatg agcaaaccaa
    15601 tggaattgtg tttgttcggg ttccatgctt cggcaaagaa ggtgttgcgt tcgaaattac
    15661 gatcgaattc gtcattcatt ttctcctgaa tatctttgag taataattga aaaagttggt
    15721 taattgtttg cattttaaaa atttatattg tatgtttgct ttcggttgag gggaacatca
    15781 ccccccgacc ccgaccttgg gattagctgt aaaagttaat cccattgtat ttttaacata
    15841 ccggacaata tttcctccct gagatattgt ttatcaaaaa atatcccgga agatgacaaa
    15901 aaattgtgca aacatttata ttatttattt tcagatattt aatgatgaaa atttgccaca
    15961 aaccccacac aagaaaaatg ggcgttcgtt ggctaaaatc ttttaaactc gctccttcgt
    16021 cgctcaaaca gaaaagattt ttttaacgcc aacttctttt tagctacgct gaccgttggt
    16081 tcggctcacc ggctcaggtc gcttgtttgc ttcaaacacc gttttgtcgg atttgcaata
    16141 tgacaggata ttgccggtgg aggcataaaa tcagcggaaa aaatcagcgt aaatcagcat
    16201 gagaaaaacc tctctcgccg atgattgctg ataaatatcg cagataaacg cagaaaatca
    16261 ataaaaatca ccggaagtct gcgtgagaaa ataaatgtgg gtttattcaa accttattta
    16321 ccaccacttt aataacacaa caaatccacc acccaaattg aattttatga gcttattaaa
    16381 aattaaaaaa tccggagatt ttatttttac ctgctacgca ggcaggaaga tatgacaaat
    16441 agaaaatgtt caactaaaaa atgtttgtcc ttttcttcgt ctgttttaaa aatttaacgt
    16501 tatatttgtt atctttttag caatacctca atttcataaa aacaactgca acattaaata
    16561 aaagcaataa acacaaaaat tttatgactc atcaaaacgg acttgacgta aaaacgctcg
    16621 aaaactggtt gtgggaagcc gcttgcaaaa ttcgcggacc cattgatgcc cccaaataca
    16681 aagattacat tttaccgctc attttcctca aacgactttc cgacgtattc gaagacgaaa
    16741 tcaatcgtct ttcgcaaaat tacggttcca gagaaatagt ggagcaattt ctatccaacg
    16801 accataatct tgtgcgtttt tatctgccgc ccgaaacccg ttgggaagcc atagctaaac
    16861 atactacagg cgtgggcgag tatttgacca atgcggtgag aaaaattgcc agagaaaatc
    16921 ccaaacttca aagcgtcatc gacattgtgg attttaatgc tacagccggc ggacaaagaa
    16981 ttatcagcga cgaagttttg aaagacctta tcaacgtgct tggaaaatat cgattgggat
    17041 tggacgatgt ggaacccgac attttgggac gggcttacga atatttgctc agaaaatttg
    17101 ccgaaggttc gggacaaagt gccggcgagt tttatacacc cagcgaagta gccgttttga
    17161 tgtcgtatat tctcgatccg aaacccgggg acgaaattta tgatccctgc tgcggttcag
    17221 gcggcttgct catcaaagct tttttgcgaa gcaaggaaaa attcgacgat aatccctcct
    17281 tcgaaccggt acgattttac gggcaggaaa ttctgcatgc tacgtatgcg atggcaaaaa
    17341 tgaatgcttt tattcacgat atggaagccg aaatagcact gggcgacacc atgaaccgtc
    17401 cggctttttt aacgtcggca ggtgccctgc ggaaattcga cctggttacg gccaatccca
    17461 tgtggaacca ggatttttct caaacggttt atgaaaatga tccctacaac cgtttcgtct
    17521 ttggttttcc gccttccaac agtgccgact ggggctgggt tcagcacatg tttgcttcgc
    17581 tgaacgacgc cgggaaaatg gctgtggtgc tcgatacggg cgctgtttca agaggaagtg
    17641 gcaatgccgg cagcaatcgc gaacgcgata tccgcaagga atttgtcgaa agagatctgg
    17701 tggaagcagt catcctgtta cctgaaaatc ttttctacaa cactacagca ccgggaatta
    17761 ttctggtaat caacaaagcc aaaccagccg accgaaagga acatttgttg ctcatcaatg
    17821 cggctcatct gttcgaaaaa ggccgaccga aaaactatct gcctgacgaa agcatcagcc
    17881 gcattgccga tatttacctt caatggaaag aagaggaagg catcagcaaa attatcacca
    17941 accaagaagc agcccgaaac gactacaacc tgagccccag ccgatacgtg gcacaaaacg
    18001 gcgaggacga taccctgcca cccgaagatg cggtgattca gttgcgagaa gcggaagaag
    18061 aacgacaaaa agcagatgaa aaactgaaag caattttaca agaaatggga ttatggaaaa
    18121 attaaaaaat caaatgaaaa ttagttcttc tccgggaaag aaatagataa ttcaatatat
    18181 tgattataag ggttcataga ggagaaaata aagataattg aaagaaagaa tgacatacaa
    18241 tccaaataac catcatcgcc gttccatacg attgcatgga tacgattatt cgcaaccagg
    18301 tatttatttt attacccttt gcacatataa tcgtgaatgt ttatttgggg aaattttaaa
    18361 tggtgaaatg cgattaaatg aatttggaaa aattgcaaac caatgttggt tggaaatacc
    18421 caatcatttt ccacaaaccc aattggatga atatatcatt atgccagatc atattcatgg
    18481 gataatcgta ttgaatgatt tcgtaggggt tcaaaatgtt gggacgaaaa atgttgggac
    18541 gaaaattgta ggggcgaaaa atttttcgcc cctaccaatg ccaacgcccg aaccaacgcg
    18601 cgaacaaaat atcacgccat ttcgttcgcc atcaaaaacc attggttcca ttattcgtgg
    18661 atttaaaatt ggcgttacca aatggttccg tcaaaatacg gatatttacg ttgtttggca
    18721 acgaaatttt tacgaacaca ttattcgaaa tgaggatgaa ttaaatcgaa taaggcaata
    18781 tataatcgat aatcctaaaa aatggaaaac agatgaaaat tagatttgat agggcgaaaa
    18841 agaaaaagtc tccggcaacg tttagataat gttagggcga aaaatttttc gccccgacta
    18901 taaaaattaa acaatgattc tatgattatg aactcaataa acgacgaaat attactgcca
    18961 cagggctaca aaatgaccga actcggacca ctgccagaag aatgggaagt ggtaaaactg
    19021 ggagaagttt gtaaagtgga ttggggaaat acctcgttaa ctaaaaaatt atatacagca
    19081 aatggttatc cagcattcag tgccgcagga aatgatggtt ttttagattt ctatgaacac
    19141 gacggtgaag ctgtgatatt atcagctatt ggagcaagat ctggaaaatg tttttatgct
    19201 tctggtaaat ggacagccat taaaaatact attgtaataa agagtaataa tgaaacaaaa
    19261 attaatctgt tatttttata tttttttgta aacaatgaaa attactggca taaatccggc
    19321 agtggacaac cattcataag cataggtgta gctcaaaaac aaaaactccc tctccctcca
    19381 cttcccgagc agcagaagat ggcagcggtg ctttctgctg tgcaggaggc aaaggagaag
    19441 acccgggcgg tgatagacgc cacaaaagct ctcaaaaaat ccatgatgaa acatctgttc
    19501 acctacggac ccgtgtcgcc ggaggaagca gaaaacgttc cgctcaagga aacagaaatc
    19561 gggaaagtgc cggaagaatg ggaagtggtg aagttgggag aactcgccga ggtgaagtat
    19621 gggaaagcat gtcccaaaga acaaggtaat gttccagtaa ttgggtcagg aggaatttat
    19681 ttttggacta ataatgcgat tgtagaattt cccactataa taattgggcg gaaaggtacc
    19741 gctggaaaag tttggttatc acttcagccg tgctatccat ctgatacaac attttatttg
    19801 aaatggctcg aagataaaat taatataaaa tttattttcg agtacttgaa tattaatcaa
    19861 ttatcaggag aacatgcaaa aacaacttta cccagtttac aaaggcacga attagaaaaa
    19921 tttcttctcc cccttccccc tcttcctgtt cagcaaaaaa ttgcttcgat tctttctgcc
    19981 attgacgaaa aaattcaggc agaagaggca aagaaaaaag cactggaaga tttgtttcgc
    20041 tcgttgctga gccatttgat gagcggaaaa ataagggtga aggatctggg gattggtaat
    20101 tgttgattag tgattggtga ttggtgattg gtgattagtg attggtgatt tttttgtaaa
    20161 acaaccatta taaaatacac aggatatggc aaaattaggc ggagaacgat attcggttca
    20221 acaacccatt attcagtatg tgcaggaacc atcggcagca tacgtttcgc aggatggact
    20281 gaccatgttt ctcaaactcg gctgggaata tgtaaaaccc gacgacgcac tgcgcctgaa
    20341 aggcggcgaa gccggcatgg tatttcgtga aatttttatt cggcagctcc agcgtctcaa
    20401 ccccgacttt ctggatcacc tgatggcaga agaactgatc aaaaaaatcg aaacagtgcc
    20461 tgccaatatc gagggaaatt ttctgacctg ggaatatctg aaaggactca gaactgtttt
    20521 tgttcccacc gaaaaacggg aacgcaacat aacttttatc gacgccgaaa acatggatcg
    20581 caatactttt catgtaacgg acgaatttgt gttcagcaac ggcatcaaaa ccattcggcc
    20641 ggatattgtt tttctcatca acggcattcc cgtcctgttt atcgaaacga aagctgccca
    20701 taaaatagaa ggcatgagcg aagcattgga acaaatcaag cggtatcatc gcgacggtcc
    20761 cgaattgctt gccgtgctgc aagtgtatgt cttgacggat atcgtcaaat atctttacag
    20821 tgccacctgg aacacttccg aaaagctcct tttcaactgg aaaaacgaag ccggcggaga
    20881 ctttgaacaa ctggtcaaaa cctttttcga ccgcaaaaat gtaataaaac tgttgacgga
    20941 ttttattctc tttacccgtc aggacgacga actgcgaaaa atcgtattgc gtccgcatca
    21001 aatgaaagcc attgataaaa ttgtgaaacg tgccgaagat ccacaaaaac aaaggggact
    21061 gatctggcac acgcagggct caggcaaaac ttttaccatg attgttgcag cacagaaaat
    21121 tatcgaaaat ccacttttcg aaaatccgac cgtcatcatg ctggtagatc gcaacgaact
    21181 ggaaagtcag ctttttggca atctcgctgc cattggaatg agcaacgtgg ttgccgacag
    21241 caaggaacat ttgcagaaac ttctttccgg cgaccggcga ggattaatcg ttaccatgat
    21301 tcacaagttt gaaggcatgc ccaaaaatat caatttgcgg aaaaatattt ttgtgctggt
    21361 ggacgaagca caccgaacga ccggcggaaa gttgggcaat tacctgatgg gggccattcc
    21421 aaatgccact tatattggct tcaccggcac accaatcgat aaaacacagt acggcgaagg
    21481 aacttttatc acctttggca aagacgatcc ggaaaatgct tatctcgaca aatacagtat
    21541 tgccgaatcc attgccgacg gaacaacggt gcctttatat tatacattgg ctcccaacga
    21601 attgcgggtg gaaaaggaag tgcttgaaaa ggaatttctg gagttgaaag cagcggaagg
    21661 aatgagcgat gtggaagaac tcaacaaagt gctggaacga gctgttacat tgagaaatat
    21721 gctcaaaaac aaggaacgcg tggaaaaagt agctcaatat gtggcaaatc attttctgaa
    21781 cacagtagag cctatgggtt acaaagcctt tctggtagga gtggacagag aagcctgcgc
    21841 tttgtataag gaagaactgg acaagtattt acccgcagat tattcgcaag tggtgtacag
    21901 ttcgttttac aacgaccccg aagaactggc aaaatatcac ctttccgata ttgaagagaa
    21961 aagaatcaga aaagctttcc gcaagccaga cgaacttccc aaaatactga ttgtaaccga
    22021 aaaactgctc accggtttcg acgcacccat tctctattgc atgtatctgg acaagcccat
    22081 gcgcgaccac gtattgctgc aggctattgc ccgcgtcaat cgtccttatg aagatgaaga
    22141 agggagaaaa aaagtgtcgg gtttcgtact cgattttgtt ggcattttcg atcatctgga
    22201 aaaagccctt gcattcgatt cgcaggatat tgccggcatt gtgagagatg tagaaatgct
    22261 gaaggaaagg ttcaaagaag agatggaaaa ggcaaggaaa aattacctga cccttatcgg
    22321 cgggaaaaag caggataaag cagtggaaac tttgctgaat tattttctgg atgaagaaat
    22381 caggaacgaa tattatcggt ttttcaaaga attatcaact gtttacgaaa ttatttcacc
    22441 cgatgctttc ctgagggatt atctggaaga ttacgaaacg ctttcgagaa tgtaccaaat
    22501 tttgcgagaa aattacgatc gtggcatcga catcaataaa gattttaccc gaaaaacgat
    22561 tgaactggta aaaaagcata cgaaaagcgg agaaattcag cctaccatcg atatattcga
    22621 aattaatgaa gaaacgctgg aagaaattga aaaaagcaaa acctccgata tagaaaaagt
    22681 tttcaatctt attaaaagta tagagcgaac cgtagcacag gaaggcaata cagctcccta
    22741 tctgaaatcg attgccgaag aagccgaaat gctggcaaaa ctttttcaag aaaggcaaaa
    22801 aactactcaa gaaacgctcg aagagctgaa aaaaatcatt gaagaaatcg ttacggccaa
    22861 aaaggaacaa gtagagaaaa acatgacaag tgaaattttc agtatttatt ggatattgga
    22921 cagatccggt tttgaaaatg ccacttcaat ggcaaacgag atggaaaaag tatttgaaga
    22981 atatcctcac tggcaaaaga gcaacaaaca cggcataaaa atcagacaaa agctttacga
    23041 agtattggtt cattcgggaa ttaccgatag taaaaaaatt tcagaaaccg ctcagcaaat
    23101 tatgaaattg ttaaacgtca aaatttaaaa tgaccacgca cgaagaatta aaaatcgaag
    23161 tgatgacttt ggctgaagaa gtcggcgtaa agcccaatgc cattcatatc agagccatga
    23221 aaaggaaatg ggcaagttgt tccagcaaag gtcggttgac cttcaacgaa acattgcttg
    23281 acgaaccgaa ggaagtacag tataaagtca ttttgcacga attgctccat ctgaaatatc
    23341 ccaatcacgg caaaatgttc aatatcctgt tgaaaagtta tctggaaaaa atcagtcccg
    23401 ataaaaatta gattttctct gtcacaaaaa atggatagaa aagacgcctt agaaattttt
    23461 ggcaacaaca tcagtcgcct gaatgttaca cggaacggag aaatcagcat ttatcagcag
    23521 aaaaaatcag cgtaaatctg cgtgagaaaa taaatgtgaa tttattcaaa ccttatttta
    23581 ctaaaacctt attaacacga caaatccacc accctaattg aattttatga gcttattaaa
    23641 aattaaaaaa tccggagatt tttgttgctg atgaatgctt gtagtagtaa ggaatgtatg
    23701 taaagaaatc cttgtttttg tggaaggaag ttggtttatg agttttaaag gatgacctta
    23761 ttgattttta tttcaattcc tttgggtgcg attgcgtttc ctgtgataaa tgggacggat
    23821 gcatacatct gtcttggata tgcgaaaagc catttcaatc tgccaaagat tcttgtagtt
    23881 tttcattact tcctctatgc aatacatgtg ggtttcgtaa tcaatttcta cactgatttt
    23941 accactcaac ttcagatatt tgttatatcc cctgttgttg atattcgatt tggtcaattt
    24001 gcccgatttg atctgcttct caattctatc taaaccttgt ttgcgatcgt aacagtcctt
    24061 cttggctctg gcagttgaat agtttactat ccatcgaata ttgtccgatt tttttatact
    24121 caccgtcatt ccatcagtaa attttatggc aagtattttc tctttgatcc tttcgttttg
    24181 gttcttcacc cttgcttcca aaatatattc atattcgtta gactccagtg ccttaatgtt
    24241 ttctttggag agaagacctg catcggctat caccacaggt ttatttaaat gaaacttgca
    24301 gctcatttct tccagaaaag ggatcagcgt atgtccttcg tagatgttcc cttcaaaaat
    24361 atcataacca agcgacacaa cattaatttt gccgcctaaa acttcaaggg tatgggcaga
    24421 cgaaggtaat caactgtttt taacttgctc aatggaaacg ctaaccgggc tataaccaaa
    24481 tgcctgaaaa gctcctcttg aatggaatta aaaccaataa aatcgtaaat cttgccaaaa
    24541 attaactctg gtccaacagt atgaatactg ctattgtcaa gcagagaaaa agcttgctca
    24601 atcatctcat cgctttcgta tccaaatagc ttcaattgat ctccacttaa acgatccatt
    24661 tcttctccgg ccaattgttc caacctttca atttcgtgct gcgtggtggc acaaccaata
    24721 gttttcacaa ctttatactt tcctcgaaac ttctgtatca cttgtacaga ttgacttcca
    24781 gatcgatttt ttacttttcg gataaacata ataataattt tgcaacaccc aaaattaaaa
    24841 tattattatt gatattcaaa tacttataag ggtgttgcaa aaaaatgcgg aaaacaggaa
    24901 aaaccatgag agaaagataa gtgaatatct aaaaactctc atggtcatgt aacaaactaa
    24961 acaacaatca ttttttaata tacttgtata cttttacacc atgtggagga atctttacaa
    25021 atgttgggta cttatcagtt tttatttctt tatgttccca taaatctcta acaatataat
    25081 tctgaagttg ataaggatct tgtgtccaat aatcatcatt tgacttaagc agaaaagcaa
    25141 aatccaccat aacctctttt atttcatcac ttctatttag taaacaaaca gctttttcat
    25201 cattagatag tttttttatc caaatctcat aatctccatt atctctaaag caaaaagctt
    25261 gtttacctaa cttatcctga tcgatagcaa ttaattcttt gttcgttaat atctctaaag
    25321 tttccttaga catatctgac agatcattgc ctgccatcaa aggagcagcc atcatacacc
    25381 aaagagaaaa atgagtttta tattcttcta tagtcatacc tccatttcca acttctaaca
    25441 tatccggatc attccaatga ttcggtccag aatagggtcc aagatctctc tgcttatcta
    25501 caatatctat cattgcatcc caacggtcaa caatatctcc tgtagtgcgc caaagatgac
    25561 ctacttcttg agcccactcc catggtcgtg attttcccca ttcacaaata ctaaaaacga
    25621 ttggtcgacc tgcggcataa agggcatcac gcataataga ataggaagct ttagcatctt
    25681 gtgtcccggt attgcaccaa tcatatttta aataatccac tccccaactt gcataagttt
    25741 ttgcatcctg aaattcatat cctctacttc ccggtcgtcc ctgacaagtt tttattccag
    25801 catccgaata aataccaaat ttcaagcctt ttgaatgaat ataatcagct aaatacttaa
    25861 ttccatgagg gaaacgttga gtatcatgtt gtatattgcc attttcatct cttgaaactt
    25921 gccaacagtc atcaaccact atatattcat aacctgcatc tttcattccc gaacttatca
    25981 tggcatcagc cattttcata atcaactcct cattaacatc gcagttaaat ttattccaac
    26041 tattccaacc cataggggga gtgtttgcta aaccatcaaa tttttgtgca attccattta
    26101 aacaaataaa cgtagtgaaa attaacaaaa aaagtttttt cattttttta aatttatgtt
    26161 aaattttatt catttattaa tcgagtctta aatagcttct tgctgtatca taatgcaaat
    26221 ttctatcaaa aagatgatct ttctttattt tcttgtaatt aaatgcagct gctggaagca
    26281 ttacattatt tttgcctgta attcttttgt ataaatttta ctattgttta tgttttccaa
    26341 aaccattttc ttctgacggt gaaaacagtt actgccatca tgccggatgt aataataact
    26401 cctttcagta ttccgaacca gggcgttatt tcgtgtaaag tattaatatg cggcattaaa
    26461 cctgtaaaaa ctaatattgg aaccacaaaa aaagtacctg ctacataagc caccatcggg
    26521 ttttgtccgc attcggaaat gtagcggaat aacttcttat tttcaaaata atcgattaca
    26581 aaagagaaaa acagcagtgt gaaaattgcc aaaccacttg taacgaaaaa ataactcatg
    26641 gtagcgctgt cttttcgtat tccgccttcg aatgcttcga aagccgttcc gagcataagc
    26701 caaaaaattc cccatttgta caaactgaaa tacaaacggc taagcgaatg ggttggtttg
    26761 cgcattaaaa tatatcctgc aatgctaaat gcaatattta gaattaaatt taacagcaaa
    26821 aatcttccga aaagacaaat cagattgctc agtacaaatg caaccatgag cagaagcgat
    26881 gctcccattt tttgtttcga tggcaatatt gatttttcat tgtccttatc agtaaaccgg
    26941 cgaaccagca aatcgccggc aatggttccg ggaataacga taaacaggta tttcagaaaa
    27001 tccatgcgat acagccaacc tcccggattg taaacgattg ttgtgattga gtcgggtaaa
    27061 agctgcattg gattaaaatt ccacaacacc tgattccacg aaccatcgac acccgatgta
    27121 agccggagcg caaatagaaa tgcaaggata cctaaccgaa gaagcagatt tgtgcgtgtc
    27181 aaaatccatg caagcgaacc gaataaagcc atattggcga gcaccagaat aataatatca
    27241 ttgtgcgaaa gtttgaaacc tttactgaaa acttcgggtt gcgaactgac attgaaggca
    27301 attaatccca tcagaaaggc gattcctaac cagttcagcc acgtgttttt tttctccgaa
    27361 acgttaggaa atcggacaaa actgataaaa aacagaccaa atgccaatat tgccaaagcg
    27421 aaattccagc ctccacccag ccggtatgga ctgcaatgat aaattgcaat ggcaaatagc
    27481 accaataaaa aaacacgttt cagaattgca ggaaaaagtg aagtaactgt cgctccttta
    27541 tctaattttc ggcctaaagc cagcggaaaa gccgctccca tagcaaaaag gaagaaagga
    27601 aaaaccaaat cgacccatgt aatgccaaaa atcgttggat cgaaattaaa attggaacgc
    27661 ggtccaacct gtgcgtgata catccaggca ggtaaagtag gggcaacagt acccgaaagt
    27721 accatgccga taatagctaa accccgaagg gcatcgagtg cggaggaacg attattcatt
    27781 aaaaaaatac ttgcaattta actatttacc atttactatt tttgatgtca aatttacatt
    27841 ttgtcctttt atgttcaaaa taaagatccc tttttgtgca acagaaatag ttttaaaatc
    27901 attggacatg gtcatattcc agttttcgct taaaatcagt tttcccgcca aatcaaatac
    27961 gcgaatctga accagctgcg cactttcgag ttgcagattc agttgcagcc gattgttttg
    28021 atcgctgaaa accaacagct tttgtccgtt attttctact gccggcacag tggttgataa
    28081 gccaagcgac ttccgcaaga gatatgtgaa tttttcgggc gtaacaaatt caacatcagc
    28141 gccaatgctt gtattatttt taatgtcatc catcaatgtt ttaaggcgtg gtaatgtaac
    28201 atcatttgta gccaatgtag cattgtcgaa acgaaatcgt tggtaaccgg ccatcagaaa
    28261 acggggcttt gttttgccac tcagtttgct tacaatccgg tcgcgtacgg tagtgaggtt
    28321 cgaaaaatcg gcataaaaag tatcgtcgtt tacatgatta tacagcaaac caccgtaatt
    28381 ggtataggct ttttgcgtca tcagtgcagg gtcaaacaac atggtcattt gtgcattggt
    28441 ttgctcggca aacaaaccta aattgctgaa attaaaatta ttggaaatag taattcctct
    28501 atattgagaa gaacctgctc cgttgtaatg cttatacgca tacaccgaat ttacattgaa
    28561 tttatccatc aatgcaatag tttttgcttt tgcatcgggc aaatacgaag tcggaaacac
    28621 atcgggataa gcataaccca gcggcgtgga cacggccata aaaccatcgt tagcagtggc
    28681 cgttcggtaa taatactgcg ctacaaacgg aaattcttcg aacatttgta aattaaaacc
    28741 ccaggcaaca ggcacttgtc cgcgggtagt cgatgcccat gcaccaccct gaaaaccgat
    28801 attccaattg cctgcatcac cctcggaact gatgaaaagc acatagtatt tatcttccaa
    28861 caaaactaat tcgggattaa tatcagccgg acgttgataa ctgaaatttg catcgaccgg
    28921 aaacacatga tgccacgaca aattgctcaa taacgtttcg tgaaaacttc caccccaagc
    28981 cgaaatccat tgcacaagcg gctcgggacg catccagcca tatacatgaa aaatttcatt
    29041 cggtcgtttg gttttcaaat aattcataac ccgttctatt ttagctgctt tttcgtcgct
    29101 gagcgaggta aaacgtaaat cctcggttcc ggccagattg agttgaaaca tacgttgatt
    29161 tacagcccag tcggttattt cccgaaggta aaatttcgat gtattgcaac gtggcaacag
    29221 attatcgagc gcccagtcaa gccaggcaaa atagcgttgt tcctgtgtta aagccgtgtt
    29281 attccaactg tgtgaggtta gttccagttt tgctgatact ggtacggttt gttgtccatt
    29341 aaaataatcg ggcaaatcaa ccgaagttgg tttatttata tcaatgattg tgttggttga
    29401 tggaaccgga atgcagtcgg taagtccgcc aagcatggca gccgcttcag cttgccatcg
    29461 aaacgattgc ccactgaagt tgctgtaagt cagcgtggca tcgtaggtaa tggctccctt
    29521 tatgtagctt ttgaaatact gaacgagagc attaatattg gtaattacgg taaactgaac
    29581 accaccaacg gttttgtatt cattttccca caattcgtca gtttggttaa aactccatgt
    29641 ctcgtacaca tttcgcaggt acaaacgagg tgcgtcacgg tttacaatcc cggcaagcgt
    29701 gagtgcaatt aatttatcct cagataactg acccgaacca tcgtaaacca ctgcatttat
    29761 ggcatccgca ttaaaagcaa tataaaatga gataagaagt aaagtgattt ttttcatttt
    29821 tttaaacaaa gagcaaaaaa tttaataata aaaatacaat aaacaaaaac atgaatcccg
    29881 aacgacgatc tattttttct ttacaaaaga aacaactgtt gccacaccgc gttcagttcc
    29941 atccgaaggt ttgttgagta attttccgcc tgcaataata gcagaagaac cgccaccatc
    30001 aagattaagt gcatttacac aaccaatacc tttcataatt tgctccaatt caatcaaggt
    30061 agctccatca ctatgcgttt gtctgccatc gcaaacaaaa agaacaattt tattatcggc
    30121 agtgcttcca attactgttc gtggcggacg agctgttgtg gagaaaatat cagtttgtaa
    30181 aagttcataa tttgtcatgt attttcccga agttgtagtt gtaaaatcga aaacaatttt
    30241 tccatctttc agcaaaacag gagcaccacc aatggcagaa taaggattcc agttctgtga
    30301 ctgggcaggg aaactagccg acaatgatgg ttgcggagtt tcaccctcca cattcggact
    30361 tggaatatca taagcaaaat tgcccaccgc ccatttgata gatggagttt gattttgaga
    30421 aacgccaaat gcaccacgcg taacagcata ggaatggctt ccacgtgtca atgtcgaaat
    30481 attatccgat ttttttgatg ttctgtctac agccagacta taagagttga cagttgaacc
    30541 actgtatcca aaatagccgg cattggtcat tacatatgtt gtttcattgg tagcagcagc
    30601 aataaactga cttggttttt gaggcgaagt ggataatgtt gttttcaatt caataattcc
    30661 ggtgctcata tctgcaatgg cataccaggc actgaaaggc cttccgttta gtgatgaagt
    30721 tgtgctataa acatttattt cagtaggtaa ttctgcattt atagatgtaa ttggtgtcca
    30781 ggataacgca attgcaggga gttcagggga caaggttgcc tgaatttccg gactataagt
    30841 agttcctttc gaatttgtgg cataagctcg tatatagtaa tttgtatttt tgtctaaatt
    30901 caccgcattt ccaaaaaatg tttctcctga ttttactttt acatgccaca ctgacttgga
    30961 gtcagttatg gtaggatttg gtgatttact ccaacacaaa ccggcattac aaggttctcc
    31021 accatcggaa gtaagagtaa aataagctga tatttttaaa taagaaacta tcggtgaagc
    31081 aattgtaatt atgggcgttt tcagtatcat tagtgagcgt tccacaccaa ccgatgtggc
    31141 actccccgca taagcataaa gtttataagt gttcagtcct acggccggat ctgaatctgt
    31201 aacagaagtt gtgtttgcat caagtgtttt ccacgcttgg taaatttctg tgccaatctt
    31261 gcgttctatt ttgtaaccat caatgttttc tgttagagcg tcccatgtta gatttacagt
    31321 attatgggcg gctacaactg tgaaatttgt aggcatagaa acaggaacgt gctgataata
    31381 aaccgagaca gtatcggaca cacgttgttt gcaaaatcca taaactttgt ataaatacgt
    31441 tgcctcctgt agtccctcat cgatccattc ggtcgtattt gctccaacag ttgcttttac
    31501 gggtactgac gttccagtaa cccaacgttc gatagtgaaa ccatcttcac cattgctgtt
    31561 gtccgaccag cttattttta ccgaggttgt ggttattttt tcgacaacca attctgttgg
    31621 agcggggaat ggtgcctttg gattactcaa atcaggctcg ttgcaagagt aaaatgttga
    31681 aaacatcgga ataaggagaa aaagcttgta ccttattttt gttggactca caaacatggt
    31741 ttattgtata gtaaattaaa atggaaaaat tgccttgccg ggatacaaag ctttatatac
    31801 tttttgtttt gatggaatag cttcgtaaaa ccacgtggac tcaccataaa cacctttagt
    31861 acggttatat tgaattaatt gtgccaaaaa ttcatcagag acaattgtat ttccattgcg
    31921 taccaacatg ccaggaacat actttttacg taaatcagct gtgttaaaaa cattttgtac
    31981 atcgctataa acaagggttg tatacgaatc aactccctgc gattcgttac gatacaattg
    32041 aggcgaaatc caatcagcga cacctcttct gagccattca ggccagtcct gcaaatagtt
    32101 atccctactc cagccacgtg tacttggagc ccaagtaacc agacaagtag aacgggcagc
    32161 tttcaccgca ttataaagac taacagcata atcgctcatt ttattgactt tccaattaaa
    32221 aaaaatggtt tctgtggggt tggatggaac atctcttcct gtttcagttt tataaagttg
    32281 tttggtataa tcgtcgtatc cagcatgtgc cggaagagct ggcaggcggt cgtcgccctg
    32341 aatgccatcc aattctggat attttgtaac gcattccaca atcatatcgg tcataaattg
    32401 ctgcacttcg tgatggaacc cgttgagcca gtaaaaactg tttttaaccg tttgtgtccc
    32461 atcctgttgt ttactcaccc agtccgggtg tttatctgag ataggtgaat cctgacctga
    32521 ataatgtgaa gcaaatccat attcgaacca ggcaattacc tttatatttt tagcgtgagc
    32581 tttttcaatc agtttttcta atacatcgcc ccacgagcaa ccagaaaata ctgtttgtgc
    32641 aggtgttgtt cccaacacgg tagccagcgt ttgactgggg aaaagagttt gacttttatt
    32701 gtatgtacaa acacatatcg tgttaaaatt gagttcggcc agtttatcta ccatatccgt
    32761 aagagctgat tctgaaaata aaactgagct tgctacattt gtaacccaaa cagctcgtac
    32821 gcctgtttcc tgtggttgaa tattagctgt aatagtatat gtttttgaga gattcccaat
    32881 tgataatttc cacaaaaacg gttggcttaa atcgactgtt gaacccgaag cgatattgga
    32941 tgttaatcca ttgtctgcta acagataagt caacttcatt gccgaaacat ttgttccaaa
    33001 aggaacagta aatgtaatat tgtcgccatt gatagttccg gttacaaaat tatcgggaag
    33061 tttgacgtta ttgactctga aagtaggttg tgagtatacc agaactatgt aacttttttt
    33121 tgtacccaac tcggaagtta cagtataaaa tacaggtttt gaaaaatcct gtttaacacc
    33181 tgaagcagga ttaatggttg cttttactga aacttcaatc actggtgtaa gtgaagttaa
    33241 atcagtgttt gttggcacca taatcaaaac tgtatcgttt gaaatataag cctcattggc
    33301 ttgtgaaatt tcctgaaatt tgaaactaat tatttcattt cctccctcca atgattgttt
    33361 gcacgaaagt aataaaatac aagtgaagaa taaaaaaaat tttttcatca gaatataatt
    33421 tactaaagtt tcgcacttcg ttttaatgat ttgaaagaca ggaaataaag aagtgcgatt
    33481 aagtcatttc ctgtctgatt atcaatatat taataattcc attttcgtca aactgcttcc
    33541 ctgagcggag gagtaagcat ttttgaaacc aattccgccg tcttgggaca attcatgatc
    33601 aactcaaaca attcgtcaat atctttctca aatgcttcac atatttcctg caacaactcc
    33661 aaaaataatc cccaaagacg ttcgtcaagc gtttgctgca aacattccgt attcattacc
    33721 ctgaagagtt ctcccatcga ttcataatgt tcataccgat atcgaaaaga cagtaaaata
    33781 taggctatca tgcttatcgt ggtatcggct atctgtgcat cgaaatcatt ggattgacat
    33841 cctcccagat tcaacaattg cttcgtttct ttgaaaaata cttctatcga ccaccgtatc
    33901 tggtagattt ccatcagttg cacaaaactc aacttcgtat cggtggttaa aaatactttc
    33961 catttccctc tcttgccttg acgggtaaag aataacgtta ccgcaatacc atccaacatt
    34021 acatctgcac gcttgtagtg cagtttcagc tttctgcaac gggttatttt atcaattgaa
    34081 ctttgtatct cccggtaatt catctgcttg ccctgataca gaaattttgc ttttactatc
    34141 ctgtacatgc ctatcaggtg aatatgcttt gaacccactg ccgtaattaa tgcctgacag
    34201 gtaaaccaac tgtcgcacaa aacataatcg aggggaatgt gataaaatac agcccggtaa
    34261 aaaatctcaa gcatcatttc tatcttgctc ttatccaatt ctttctcccg ttctctccct
    34321 gaaagctcct tcaaacgctt tttgctgtat tgatggcgta attcctttcg gctcattccg
    34381 taaggcttct ctgcccgttt ccctttctct cttgaaagtg aaaaattcaa tggaatcatc
    34441 gactttccgt cccaatacaa tcctaacaac agcttgtatc ccaatttgaa agtgtttgtt
    34501 acatgatcgt aaactttccc gatcttttcc atctttttcc ctgtcttttc aagcagggtg
    34561 tcatcaaata tgagacatcg taccttacat gatacttctt tctcctgtcc ggtaatgccg
    34621 ataaacttac acgctgtctg ccataacagc ttccgccaat tgatccctgc attgttcttc
    34681 aaacggtaca atatgttctt tcccatcgta atgccttttt cctttagcag gggcagcgaa
    34741 gatactgccg tttttcgtcc cggcgtcacg gcataaatca ttgacgacaa taccaactga
    34801 aaactgtaac cactcctttt caaatgcttg aattccgaaa atccttctgt cagtttcaac
    34861 gccttgaaac ctttcaaaat atttatactc tccaatttat tttcattgaa atctgttcca
    34921 atttcagaaa ttttttcctt aattttgtcc ggtagcatat ttttttctgt tttgtaatta
    34981 tacaactaat atactcattt ttaatgatat attagataaa atatgctact tttttttact
    35041 tctttaactc tttttcctct ttattttcag ccctttctga aatgagaaac ttttataatt
    35101 tattttttaa aggtctgatg gaaccctcat gtctgaatat tatattcatt cgtgtttgtg
    35161 tatgcaatac atgtgggttt catataaagt attattgaga acaaggccca cacgttaacg
    35221 cgtggacttt gttggaaata gaattaattg caaatttttc aattactaat taaaagtcag
    35281 ttctttcttt ttgctgaatt tagttacaac aataccacat ccggtaccca aagccacaac
    35341 tttgaaaccc agaccatcag catcaagaat tgcatctata catccggttg catctccgtt
    35401 ttttaatgtt ccatcagctt caatcttttc aggctgcgga gttagccacc agttattgct
    35461 catttcaacg ctgtttgtgg tagttgtata atcatacagt cttgcattta cgctgtgagg
    35521 agttgcataa gcctcaacat ttaagggaat gtaatatttt acattattaa actcaagata
    35581 agttgctatt cctttaggtg ttgctacagc tgtagcaaac atataagttt gtttagtaat
    35641 tccattcata actccaactt cagatcctgc acaagcagca tttaccaaaa tatcgctggt
    35701 tgctgtagcc ccaagcggaa caattgaagc ccacaattcg aagcgcgaca tagaaccacc
    35761 cggtgtgtaa tgaataaact cgggactcga agcggcaact ccgtcagtta ctttccaacg
    35821 aagaatgtcc agtgtataat ttgcataggt ataaatataa gcttgagttt ttaaatcacc
    35881 aataaccgaa atacgtcgtc caacatggtt gccagcccag cttggtggag tccccaccgg
    35941 tgtcaaatcc gacacattcc atttgatcag ctcacgtgga gcggcgtcta cgttatccca
    36001 acaccatact ttaaagtcac cacctggagc tgccaaatta catccgataa aactgccttt
    36061 acggtctcca gccattgcac gaatttgttt aatcgtatcg ccgggagcta ctactaattt
    36121 agcatatccc gtttcggagc catcaagcaa tgaaactttt accagtttta atgttccacc
    36181 atataatccc cctatttgtt gaacaatatg agttggggtt aaagcaaatg ttttttcatt
    36241 attatcagta aacttatctg taaaagctgt tccattttta cgccagtatt cagaaattac
    36301 gccataccct ttctgagctg tttcaaaaaa acgaactttg tatgccgttt tcgaaacacc
    36361 atcttcagcc gtaacggtgt aatacaccgg agttgctgaa ttaaatttaa caggtgttcc
    36421 tgatgccggt gaaactgttg ccccttccga tactgtaagt gttgcggcgt tgctgttcaa
    36481 aagtcgattt atcgtatctg gctttaaacc aaatggtagg ttaatctgaa tttcttctcc
    36541 tacaatttcg acagatatac tggctttaat agtttgatca gctaaagcat ccaacgaact
    36601 aagatctagg gcaaatgatt taatttcaga gtcagttttc agaaccactt ctggttcatt
    36661 tttgcatgat gcaagtgata aagcaacaaa aagcacaata gcaattttgt taaataaacg
    36721 gtttgttttc attgtaacta aatttaaatt gtttaagtaa attatatttt gaaaattatg
    36781 aattgttaat tattaattat ttacgtccca ccacattctg ctcaactgat aatcgccacc
    36841 catacggttt gccgcttcgg ttacgtttgc tttgttaagc aggtgttcat cgctcggata
    36901 tttccaacgt ctaggtaatt ccatttcgtc aggaacagat gaagtatact caattggtaa
    36961 aattttagga taacctgtgc gacgtaagtc agcgtaagct tcgaagccgt cagtataatt
    37021 tcccaaatat ttttgtaaaa taatttgttc taatttttca gcttttgtgc catttgactt
    37081 aaatttagca gccccagcta aatatttatt tatttcactg tcaggcactt catataattg
    37141 catagctgct cttacaccat cattataatg ttcttcagca gttttaggag ttgtccagcc
    37201 attcaggttt ccttcggcta ataaaaaaca aatttcgggg taaacaaaaa tattggttgg
    37261 ataattgtcc tgacgcaaat actgactcaa atcagaatat tcaccaagtt tactttgcaa
    37321 tgtagttagt gattcgatgc tttgaccatt ccgtaatccc ctgtatttta tacttctact
    37381 tatttcagaa cttcttgaag gcaaaacacg ggcaacaatc gcttctattt cgtcggtaaa
    37441 ataacgtccc tgggcaaaaa tattgataaa agacccgcct tctgcagctg cgtaacttac
    37501 ttttgcttgc tcggcatacg acaatgaatt ataaatagta gaattttgtt caatatcctt
    37561 acatatttgt ttcagagcat tcaggtaatt ttttgttggt tctgcaaaat tgaatattcg
    37621 aggatcatct agctcgttaa gagttttaac cataaattcg ctaatgcggt accagtcgaa
    37681 cgcgttagcc attactttta tcggattgtc gctgtgtatt ccctgaggtc cggccggatc
    37741 agttttaagc cggcaagatt gcagattatt actcattaac cctcctgcgt ctgctaatgt
    37801 ttcatcaaac catgttttag caagtgtggg attaactttc gaaattcgga ttgccattcg
    37861 taagcgtact gaattgccaa atcttttcca tgagctgtag tcgccattat aaaataaatc
    37921 ggcactgccc attccatttc cttttgttgc atctattttt tgtacacttg ttttaagcga
    37981 attataaatt cctatatata tatcttcctg tttgtcgtac tttggataat ctatcaaatc
    38041 attgaaagcc tcgctgtaag gtatatcacc aaaaaggtca gtcaattgca aaaacaaata
    38101 agatttccag atattacaaa tggcttcttt attttcccaa cctttctgtt ttgcaatttt
    38161 ttcggcttca gccacaaacg aaagatatgt ttttgaggca tcgccagcat aagctgcact
    38221 ccacaaagtc ccattccagg ctgcgttaaa ttgatacctg tcggtacaat gttctttagt
    38281 gtaatttgta ttggcgtaca tttgtgtatg gaaacaagcc ataccacggg tgtaataata
    38341 aacgcggtcg tccattgcac cacgacgttg agaagtggtg aagaggtaag ccggatcaat
    38401 cgttgtagat gcatttgggt tcgtgttaat ttcggcaaag cggtcggtac atgccgaaaa
    38461 aacaactgct acaagtaaaa taaatattgt atttttcatt ttctattaaa tttatatcgt
    38521 tgttatttaa aagcttacac tcatgcttgc tccccattcg cgggttgatg gtatggaact
    38581 atattcaaac cccattccgt taaatgaagc ttctgggtcc aaaccttttg gagctgcttt
    38641 gtaaatgtag aataaattcc ggcctaccgc agctatttcc aggttattaa ttgttagtgg
    38701 ctttaaccat ttttttggta atttataccc aagtcttagc tctttaaatt tcacaaatga
    38761 tgcgtcgtgc aagaattctt catctacacc ggctgcatta atccagtatg attgtggtga
    38821 aacgtacaaa ttacggtttg gagtgtagct tccatcaggc aattgaatca ctccttgcgg
    38881 taaatatcca cgctgattac gcgaggctaa ccattcaaca ggagatttgc cggcagcaat
    38941 acgggcaatt tccgaagctg cccattcttc acgtccagtt gttgtccagg gtgcatttcc
    39001 atattctgta gtaatttttt ctgtaaaaga atataacttt cctcccatac ggacatcaat
    39061 taatgcactg aaattaaaat ttttatgact taaatttgtc cctaaacctc ctaaaaagtc
    39121 attttgaata ttcccaaggt aattgtctgt acgcgaacca atcgaatctt cggcaagcat
    39181 tggcaaaccg ttcgaaccaa ttatacgttc tcctttgtcg ttgcgcaggt attttgttcc
    39241 gtaaatttca ccataacgcc caccagcctt tgcatgaatt gacaacgttc ctgccgaagc
    39301 aattatacgg cggtctttct tatttggatc aagcagttcg acaattctat cgcggttgcg
    39361 ggagaaatta acaaacacat cccaatgaaa atctttttta ttaaagatat ttgtagtcaa
    39421 ctgtagctca actccctgat tttgaaccaa acctgcattc atcaacttgg tagtataacc
    39481 cgaaggtgat ggaatgcgtt gtgcaattat ctggttcgat gtacgattca gataataagt
    39541 agcatcaagc ttcagaagat tttgaaagaa atttgcttct aaaccgactt ccgaactatt
    39601 catcatttcg ggtttaagtg taacaaatgg ttttacattg ggtttatagg catattgctg
    39661 accaagatgt ccgggaccaa tggaataagt ataagaggta cgataaggac ttgtgccgtt
    39721 accaaccctt gcataagcta atctgacctt cagataagat aaaaagttgg atttaatagc
    39781 agggaaagcg tcagtaaata gtgcagacaa acttgctgat gggtaaaaat aatttaacac
    39841 ggatgcagaa ggatttatgt tcgaatataa acttgaattc caatcttcac gggcggtaag
    39901 atccaaaaac aaatagctgt tatatcctaa agaaacagtc ccgtatagcg attgtacttg
    39961 cttctgacta aatccttgtg aacttgtaac attttttgca ttacctactg tgtacaaatc
    40021 gtcaagtgaa agggccaatg cataaaaacc aatactgttt ccccatgtat gcataaaatt
    40081 acctccaccg gtcgcaacta cagaaaattt attgtccata aaattatttt tgtaggagag
    40141 caataaatca aagttatttt caaatgattt tccccaattt tgagcgtatt tcgaacgggt
    40201 ttcgttgttc tcataagctg tacgtttggc
//
LOCUS       DAOSCH010000009        39401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_772_length_39401_cov_5.693438, whole genome shotgun sequence.
ACCESSION   DAOSCH010000009 DAOSCH010000000
VERSION     DAOSCH010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 39401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39401
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_772_length_39401_cov_5.693438"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..329)
                     /locus_tag="PLZ54_01640"
     CDS             complement(12..329)
                     /locus_tag="PLZ54_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRL-like family protein"
                     /protein_id="HOJ65675.1"
                     /translation="MKKIKKILILGGIVFFFSSCAIVKSPLGAVLYTDVKAPLTATSN
                     SSSTKVGTAEATSILGLVATGDASIEAAAKSAGITKIHHVDEQSTNILGLFAKYKIYV
                     FGE"
     gene            1316..1786
                     /gene="bcp"
                     /locus_tag="PLZ54_01645"
     CDS             1316..1786
                     /gene="bcp"
                     /locus_tag="PLZ54_01645"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOJ65676.1"
                     /translation="MALQVGDKIPEFLGIDQDNKEVRRSDFNGKKLILYFYPKDNTSG
                     CTAEACSFRDNFDILREAGYEVVGVSADSVNSHQKFIGKHQLPFRLIADTDKKLSEQY
                     GTWGEKTMYGKKYFGMLRTTFIIDEEGIIRKIIPPKEINTKEHAQQILELVKNM"
     gene            complement(1838..2188)
                     /locus_tag="PLZ54_01650"
     CDS             complement(1838..2188)
                     /locus_tag="PLZ54_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65677.1"
                     /translation="MAGENYHKVLLQYKREINNFVPSTPKTYRDAIRDDAMLIISQFQ
                     PNIDNWISELNSRNLSCYEIIQMLEANKDKIRLNNLEKKELSPDLLDAYKNNILRIMA
                     KSVLDCYLSSVFNS"
     gene            2460..3068
                     /locus_tag="PLZ54_01655"
     CDS             2460..3068
                     /locus_tag="PLZ54_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOJ65678.1"
                     /translation="MSDIYRTIATRAEGMYKDKGSKFLSFALPVENVEEIKKIIKDFQ
                     HQYHDARHVCYAYVLGNEKKDWRANDAGEPSGTAGRPILRQINAHNLTNVLVIVVRYF
                     GGILLGTSGLINAYSEAASDALNHAEIIEKTVQCHLKISFDFLQLNSIMKIIKEQKLQ
                     VVVQEIDNEKCSIELAVAQHEVSDLIKIIQNISGTEIEVLNR"
     gene            3446..4351
                     /gene="pyrB"
                     /locus_tag="PLZ54_01660"
     CDS             3446..4351
                     /gene="pyrB"
                     /locus_tag="PLZ54_01660"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOJ65679.1"
                     /translation="MSNRSLVSISDYNKEEILEILDAAADFEANPNRSTLKGKVIATL
                     FFEPSTRTRLSFETAVLRLGGSVIGFSDAATSSSVKGETLNDTIRMVSCYADAIVMRH
                     PFEGAARYAAEISPVPIINAGDGANQHPSQTLLDLYSILKTQGTLENLKICVAGDLKY
                     GRTVHSLIMAMSYFHPTFRFIAPEELKLNEEYRIFCLMNNIPFEETTELNDNINDADI
                     LYMTRVQRERFQDLMEYERVKNVYTLKNSMLENTKPNLRILHPLPRVSEIAPDVDKNE
                     KAYYFQQAQNGLYIRQAIISKVLSV"
     gene            4426..4881
                     /gene="pyrI"
                     /locus_tag="PLZ54_01665"
     CDS             4426..4881
                     /gene="pyrI"
                     /locus_tag="PLZ54_01665"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOJ65680.1"
                     /translation="MEKELKVAALRNGTVIDHIPADKLYKVVFMLHLDTCDNQITIGN
                     NLESAKIGKKGIIKISGKVFAEDETNKIALIAPNAKINIIKDFQVIEKRPLSLPDEIH
                     EIVQCNNPNCITNHQNIPTFFQVIKDENDTMLKCHYCERLIKLEEVKIK"
     gene            4952..5578
                     /locus_tag="PLZ54_01670"
     CDS             4952..5578
                     /locus_tag="PLZ54_01670"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOJ65681.1"
                     /translation="MTKTDENSSHVSWKPGTMIYPLPAVLVSCGKSADEYNLLTVSWV
                     GTICTEPPMCYISIRPERHSYEIIKRNGEFVLNLTTEKLAYATDWCGVKSGKDHRKFE
                     EMKLTPEKGKMVDAPIVKESPVCIECKVKEIVPLGTHHMFIAEVVNIQANPQYIDSKT
                     GAMHLENAHLIAYSHGHYYKLGEEIGKFGWSVRKPSKKKKVSPKIPKK"
     gene            5673..6953
                     /gene="glyA"
                     /locus_tag="PLZ54_01675"
     CDS             5673..6953
                     /gene="glyA"
                     /locus_tag="PLZ54_01675"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOJ65682.1"
                     /translation="MKRDQEIFDIIEREKQRQMKGIELIASENFVSNQVLEAMGSVLT
                     NKYAEGYPGKRYYGGCEVVDESEQLAIDRLKKIFDAEWVNVQPHSGAQANAAVFLACL
                     NPGDKFLGLNLAHGGHLSHGSPVNSSGILYQPLQYNVKADTGRVDYDQMEEIALREKP
                     KLIIGGASAYSREWDYKRMREIADKIGAILMIDMAHPAGLIAAGLLENPLKWAHIVTS
                     TTHKTLRGPRGGIILMGKDFPNPWGKTTPKGEVKMMSALLDSAVFPGIQGGPLEHVIA
                     AKAVAFSECLQPEFFEYQKQVKKNAEKLANELISRGFNIVSGGTDNHCMLVDLRSKFP
                     DLTGKMAENALVKADITVNKNMVPFDSRSPFQTSGIRLGTPAITTRGAKEDLMTEIAE
                     MIETVLQNADNEKIILQVKEKVNKMMKDFPLFAY"
     gene            7086..8081
                     /locus_tag="PLZ54_01680"
     CDS             7086..8081
                     /locus_tag="PLZ54_01680"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOJ65683.1"
                     /translation="MNVKGKIAIIGGGSWATALAKIALSNEQQINWYMRRQEQIDEFI
                     RQSHNPSYLTGVEFDTDRINFTSDINKVVKSSDVLIFATPSPFFKGHLKKLRQKLDKK
                     FVVSAIKGIVPEENMVISEYFEQFYNVSPENMAVLAGPCHAEEVAMERLSYLTVACAS
                     REKARMLADRLATNFIKTSVSDDVLGIEYASVMKNIYAIAAGICHGLKYGDNFQAVLV
                     SNAIREMNRFCNAMNAPHRDISESAYLGDLLVTSYSKFSRNRLFGTMIGKGYSVKTAQ
                     MEMEMIAEGYYATKCIHEINEKVQISMPIVDTVFQILYNRCSPIIEIRKLTEQLM"
     gene            8107..9450
                     /locus_tag="PLZ54_01685"
     CDS             8107..9450
                     /locus_tag="PLZ54_01685"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012056016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOJ65684.1"
                     /translation="MEHIQFFSDKSSVSKEKIAEYKEKAIRCISALHEGTGKGSDFLG
                     WLHLPSSIDEAQFAEIENTAKLLQKSCEVVVVIGIGGSYLGAKAVIDALSNSLDWLQK
                     VRKSPIIVYAGHNISEDYLFELQEFLKDKTFGIISISKSGTTTEPALAFRLLKTQLEE
                     QQGKAEAKKLIVAITDRSKGALRKLAEQEGYKTFVIEDNIGGRYSVLTPVGLLPIATA
                     GFDIRQLVAGAAFMESLCGMDTPFEKNPAAQYAVVRNELYQQKGKKIEILVNFHPKLH
                     YVSEWWKQLYGESEGKDHKGIFPAAVDFTTDLHSMGQWIQEGERSIFETVISIKEPNH
                     LKTVPFDPENLDGLNYLSGKRVDEVNKMAELGTMIAHVDGGVPNLKVEMPKLNEYYLG
                     ELLYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLEKPGYEQETKAIKEKLK"
     gene            complement(9572..11227)
                     /locus_tag="PLZ54_01690"
     CDS             complement(9572..11227)
                     /locus_tag="PLZ54_01690"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047334 - diphosphate-fructose-6-phosphate
                     1-phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOJ65685.1"
                     /translation="METNKSPLQIARSSYQPKIPTILSGNIALKYGEPTESVADQKEI
                     KNLFPKTYGLPIITFEEGAQVEYPEIAVGVILSGGQAPGGHNVIAGLFDGLKKLNPKN
                     KLYGFLDGPSGLVDHKYTELTSEIIDEYRNTGGFDIIGSGRTKLELEWQFEQGAEICR
                     KLKIKAIVIIGGDDSNTNACVLAEYYARKNFGIQVIGCPKTIDGDLKNEMIETSFGFD
                     TACKVYSELIGNIQRDASSAKKYWHFIRLMGRSASHITLECALQSQPNITLISEEVEG
                     KNLSLSDIVEYIVQIIVQRANHGINYGTVLIPEGLIEFIPGMKKLIQELNDLLANNED
                     FVALHTDDEKRQYIKGMLSPEASQLYRSLPKGIARQLSMDRDPHGNVQVSLIETEKLL
                     IEMVAKRLAQLKAEGVYKGIFSAISHFFGYEGRCAIPSNFDANYTYTLGIVASLLIAE
                     GKTGYMASVKNLTDEPANWIACGVPLTMLMNMEKRNQKMKPVIQKALVKLDGAPFKYL
                     EAHRDEWADEKVSYIYPGPIQYFGPTEVCDLPTKTLLIEHGKL"
     gene            11590..12264
                     /locus_tag="PLZ54_01695"
     CDS             11590..12264
                     /locus_tag="PLZ54_01695"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOJ65686.1"
                     /translation="MCKNSFRNNVLIVFMILPLALQAQDQYLAEIGVTAGNGYYLGDA
                     NEMPFKNSSLVYGSVFRYRIDPRLSVRVEWNHGTVEGKYFNSTGETLNFGNSFNAVDI
                     NGEFNFFDLMQKSNSRNSKKYSTYIFAGLGTTIYANESINTVTAFSIPFGVGFKYKVS
                     TRFNVNLQWNMRWLSSDDLEAVNDLNNAQRLNGSNPLNKDFFSTVTLGITFDFLKKPC
                     DCKSKW"
     gene            12280..12999
                     /locus_tag="PLZ54_01700"
     CDS             12280..12999
                     /locus_tag="PLZ54_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005943781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOJ65687.1"
                     /translation="MFKEKIDYTHLPKHIAIIMDGNGRWAKEKGYNRIYGHQKGVTSV
                     RRITEAAAESNIQFLTLYTFSTENWNRPSDEIKALMELLVITIDKEMEDLNKNNIRML
                     TIGDLSKLPNDAAEKIRECKAKTAQNTGLTLNLALSYSSRWEITEAVKNIVCDVEKGK
                     LTSKNISDKLISSYLSTAGIPDPDLMIRTGGELRISNFLLWQSAYTEFYFTSKYWPEF
                     DKEDFYEAIFDFQQRERRFGR"
     gene            13154..15694
                     /locus_tag="PLZ54_01705"
     CDS             13154..15694
                     /locus_tag="PLZ54_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOJ65688.1"
                     /translation="MPVIEYSNTNPKYEIAEITVTGADHYEDFVLIGFSGLSVGQVIS
                     VPGPEITEAVKRFWKQGLFSDVKIYATKIENGKVWLNISLKERPRISKINYSGLKKGE
                     IEDLEKKLNLSVGNQITPNITDRIITVIKKYMEEKGFLNTDVFVYQKDDPEKPGHVIV
                     DIEVNKKIKTSVHKIYVTGNQALTVAQIDKAMKKTNEKNLRNFFRSKKFVKQEYENDK
                     KLIIEKYNEIGYRDAYIVTDSIVPFNEKSVDIYLTINEGKKYYFRNISWIGNTVYPYE
                     FLNEKLGIKKGDLYNYNYLIKRLLTDENDAISKLYQNNGYLFFQIEPVEVHVENDSID
                     FEIRIYEGKQATINEIGIKGNTRVYEHVIRRELRTKPGQLYSQEDIVRSLRELAQMGH
                     FDQEKMNNAIDIQPDPENGTVDINYVLESKGNDQIEFSAGWGATGVVGSVGLKFSNFA
                     IQNLFNPDTYRIVPQGEGQTFSLNARTNGQSYSSFSISFLEPWLGGKRPNSLSASVYY
                     STQTAISNRYLNYINNLSSMYYGGYYGGYYNDYYGNNYNYYQTEVDPDRYMRTLGASL
                     GYGKRLNWPDDYFSFYSELSFQRFMLNNWYAGYFPMTSGTFNNLSMNLILNRSSIDNP
                     IYTRTGSLFSLGLKFTPPYSVFNGKDYSQEMSNEERYKFIEYHKWTFTGKTFTPLTPN
                     NKLVLMARAQFSYLGYYNKNARSPFETFYMGGDGMSSYTSYGTEYVAMRGYENGSLTP
                     YDTKTGIAAGYLYNKYTMELRYPLSLEQSATIYVLSFVEAGNCFNSISGPNGYNPFNL
                     KRSAGIGVRIFLPMFGMMGIDWGYGFDTVTGATKPSGSQFHFVLGQEL"
     gene            15760..16275
                     /locus_tag="PLZ54_01710"
     CDS             15760..16275
                     /locus_tag="PLZ54_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOJ65689.1"
                     /translation="MKKLIVTFCFLSVFTWLSSAQKFASVDMSYILKNIPAYETANEQ
                     INQVSKKWQSEVEALQKEAEAMYKNYQTELVFMTEEMKKKREEEIVAKEKEAQELKRK
                     YFGPQGELFKKRESLMKPIQDEVYTAIQSISKDRNIEVVFDKSTAMSVVFTTPGIDIS
                     DEVLKQLGYSK"
     gene            16334..16840
                     /locus_tag="PLZ54_01715"
     CDS             16334..16840
                     /locus_tag="PLZ54_01715"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOJ65690.1"
                     /translation="MFKKIAFLLLCVFPISLMGQEVKLGHINSQEILSLMPERTEIEK
                     TLNDLQTQWENELLKMREEYYAKIKEFQDKQATMPESIKQARQSEIVEIEQRINTFNQ
                     TASSDLQKKQQELFAPVIDKVKKAINEVGTENNYTYIFDLSTQSIVYQSPKSNDVTAL
                     VKKKLGLK"
     gene            complement(16897..17193)
                     /locus_tag="PLZ54_01720"
     CDS             complement(16897..17193)
                     /locus_tag="PLZ54_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HOJ65691.1"
                     /translation="MNKIEGEKQIVQQMIRLYCNKCHHTSSLCDSCLQLSEYAVSRLE
                     KCPFGERKTSCSSCKIHCYEPQMRKEIRQVMRFSGPRMLFYHPILFLNHYLLKK"
     gene            complement(17226..17699)
                     /locus_tag="PLZ54_01725"
     CDS             complement(17226..17699)
                     /locus_tag="PLZ54_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOJ65692.1"
                     /translation="MERLKTFTKAQFSAMTGGAVDYMVMVFFTEVFHIHYTISIAIGG
                     IVGAVVNFTLNKTWTFKSKELPYKSTGMHQLLKFALVVTNSIVLKSSSTYLITTFLKI
                     DYKFSRIITDLIISIGFNYNLQKHWVFRKISIPENNIGLSENNLPLPDNEYKLPE"
     gene            complement(17704..18393)
                     /locus_tag="PLZ54_01730"
     CDS             complement(17704..18393)
                     /locus_tag="PLZ54_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005469382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase"
                     /protein_id="HOJ65693.1"
                     /translation="MDQETKRAESLIAQGRVRTNILHSVEQKLIAYLVQKIPAWMSSD
                     ILTAIGFVGSVIVFLSFVLGAFISRYFLLLGILGFMVSWFGDSLDGRIAYYRNKPRKW
                     YGFSLDITVDWLGIILMGTGFIVYAPSYWKIIGFLFVVLYGWEMITALLRYKITEQYS
                     IDSGSFGPTEVRILISLMLILEVIVKDSILYTSALACLVLLIMNFLETGKILKSADER
                     DKKEKEAKIAQ"
     gene            18737..19927
                     /locus_tag="PLZ54_01735"
     CDS             18737..19927
                     /locus_tag="PLZ54_01735"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ65694.1"
                     /translation="MNRKKNKRIFGYPRNIFFTGLTSFLTDTSLKMVYSVMPMFLLSI
                     GASKTSLSLIEGVAESTASLIKTITGFWSDKIGKNKPFLLIGYGVSAVIIPLYSLVVS
                     PIQVLILRFVERVGKGIRTAPRDSIVAGSVENGETGKSFGLQKAMDNSGAILGPLIAF
                     ILLSAFKDNYRLIFLLAGIPSILGILVIIFGITEAKKNKESLLKKFRFGDFSKKFYFF
                     LAIVFIFTLGNSTDALLLVKANEIGIKVAFIPLVYLLTNVVSVLLSIPVGSLSDRVGK
                     EKILIVGYIIYALVYFGFGASSHIVVIASLFALYGVYSAATDGIQKAFVSDIVDENKK
                     GTGLGLYNALLGITLLPASLIAGILYDKVNSHVPFYFGAVTALIAAVLMFWFQRSFKK
                     QVVE"
     gene            20075..21307
                     /locus_tag="PLZ54_01740"
     CDS             20075..21307
                     /locus_tag="PLZ54_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOJ65695.1"
                     /translation="MVLNYIWIGFFLIAFVIACIQLLVFGNVQVFSDMLNSTFDSAKS
                     GFEISLGLTGVLSLWLGIMKVGERGGVIRLFSKAVAPFFEKIFPDLPKNHPAYGSMFM
                     NIAANMLGLDNAATPLGLKAMKEMQETNPVKDTASNPMIMFLVLNTSGLTLIPVTIMV
                     YRAQMGATNPSDIFLPILISTFISTVSGLFIVAAYQKINILDKTVLGTLAGLFIIVGS
                     ILYFFSKATQHQIALYSNFIASFVLISIIISFILAAMIHKVNVYDAFIEGAKDGFQVA
                     IGIIPYLIAILVAIGMFRAAGAMDMLIRGFTWFFGLIGWNTDFVGALPTALMKPLSGS
                     GARGMMVDAMKLYGADSFVGRLSCIIQGSTDTTFYILAVYFGSVGIKNTRYAVICGLL
                     ADFAGIVAAILIGYLFFH"
     gene            21369..22691
                     /locus_tag="PLZ54_01745"
     CDS             21369..22691
                     /locus_tag="PLZ54_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ65696.1"
                     /translation="MNEIIEYIIKFLLGDNVSDDIVQKIGYTDNEEEFGRYRLVIYSS
                     GFFKPEMYGSIHSIPSLPLKIWDDTPLLYGEPEIEEVGNVRIIHADIIASTYFLISRY
                     EEMVRRGCRDVHGRFYGKESLSYKAGFIDRPLVEEYGKLLRQQLRELGIDIPEPEKKI
                     KKIFLTHDVDQLAHYRRFRGMMGAVARGIKSPKKRAEARRAIKTFFKGIRFDPWYTFP
                     WLFKIDQSLTEAIGQERCKTVVFIKAGGGNKKEDKPNSNLAHPDYKNFIRYCKRKNIV
                     IGLHASYEAGRYPNLIAEEKKKLEHAAKTKITYNRHHFLCSREPEDMEALIEAGLKDD
                     FTLSYADMAGFRLGTCRPVQWINPATRQLRPLILHPFTIMDSTLSDKRYMNMNVFDAY
                     EYCVQLINNVENYGGEFVILWHNTMVEKKSPSYHRKLYKNIIDFLKTK"
     gene            22693..23808
                     /locus_tag="PLZ54_01750"
     CDS             22693..23808
                     /locus_tag="PLZ54_01750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ65697.1"
                     /translation="MQKIAIVCVTNDLVTDQRVHKTCLTLQKCGFFTIEVGRMLPESL
                     PLQRPYVTKRLKLCFRKGPQFYAEYNIRLFFYLLFAKVDLIYANDLDTLPAAFLAAKI
                     RRKKIIYDTHEYFTETPELVNRPKVQAVWEKIEHFIFPKLSDIITVNDSIAELYKKKY
                     KKNLQVVRNIPPTYLPPRLKTRKELGLPEDKHILILQGTGINKDRGAEEIVTAMQYLQ
                     NFILLIIGSGDMLPTLQKMTMELQLEDKVIFKPKMPFEELRQYTCNSDLGLAVDKDTN
                     LNYRFSLPNKLFDYIHAGIPVLASNLPEIKKIINRYDIGYFLENHDPKNIASTIERIF
                     ENDERYKILKKNTEKARKELCWENEENNLLQILQQYS"
     gene            complement(23949..24080)
                     /locus_tag="PLZ54_01755"
                     /pseudo
     CDS             complement(23949..24080)
                     /locus_tag="PLZ54_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032528339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            24526..25560
                     /locus_tag="PLZ54_01760"
     CDS             24526..25560
                     /locus_tag="PLZ54_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOJ65698.1"
                     /translation="MKGKNTVLLIFTGGTISMSEDPTTGALRPIDFYRLQEYLPELKQ
                     TGIEIKSIPFSPLMDSSDIQPETWKEIALIIEENFDNFDGFVVLHGTDTMAYSASALS
                     FMLQNLSKPVIFTGAQLPIGMMRSDAKENLLTAIEIATAKENGKAIVPEVCIFFEDTL
                     FRGNRTTKKNAEHFNAFNSYNYPPLAKVGVHIKYFNSYIHYPESFSKLEVKTNINPNI
                     AILKLFPGITQQNVHATLNIPELKAVILESYGAGNAPRLIWLYEELKAAVEKGIIIVN
                     KSQCSTGTVEMGKYETSLNLLNAGVISGYDCTTEALVAKLMHLLGEYHSTEIIKHKLS
                     VSLCGEMTVS"
     gene            complement(25608..26036)
                     /locus_tag="PLZ54_01765"
     CDS             complement(25608..26036)
                     /locus_tag="PLZ54_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65699.1"
                     /translation="MIIAVDFDGTIVTHEYPKIGKEIPFAIETLKRLQDEFQHQIILW
                     TVREGRELEEAIEFCKMRGLEFYAINKNYPEEGDDYAESRKIRADLYIDDRNLGGLPD
                     WGVIYQMIAANSVANPFALNGEQPAFRQTKKGFFKSIFGK"
     gene            complement(26078..26542)
                     /locus_tag="PLZ54_01770"
     CDS             complement(26078..26542)
                     /locus_tag="PLZ54_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65700.1"
                     /translation="MIGKIIKTLLLVYYSVYLAAVVAAVVGYVFIFKNYVGIEAQSSI
                     AINLSSAFILFIILSIPLSLGLFHFQVKKWLLIPDKSIRIEKYKKASIYRIIVLGLGF
                     VVGVIFAYLLRSQSIIFCAAMAAAGLIFCKPSEMKMIEELQLDKEENIPAEK"
     gene            complement(26562..27188)
                     /gene="recR"
                     /locus_tag="PLZ54_01775"
     CDS             complement(26562..27188)
                     /gene="recR"
                     /locus_tag="PLZ54_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOJ65701.1"
                     /translation="MSQQQFSSSLLEDAIHQFAKLPGIGRKTALRLVLHLLKESEEEV
                     ENFGNSIIRLRKEIVYCKICHNISDSDICDICSNLSRDKSVVCVVENIKDVITIENTQ
                     QFRGLYHVLGGIISPMDGVGPKDLEIDSLVQRVENDGIKEVILALSTTMEGDTTNFYL
                     YRKLEPFHVKITTLARGVSIGDELEYADEITLGRSIQNRIEFSQTFNK"
     gene            27314..28780
                     /locus_tag="PLZ54_01780"
     CDS             27314..28780
                     /locus_tag="PLZ54_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOJ65702.1"
                     /translation="MSGFAILLIIAAYFSVLLFISFIVSRRSKDNDAFFLGNRKSPWW
                     VVAIGMVGSSISGVSFVSVPGMVNSIDFTYMQTVFGFFFGYLIIANVLLPVYYKLNLT
                     TIYTYLENRFGKHSYKTGASFFLLSKTIGAAARLYIVALILQNLIFEAWGVPFELTVC
                     GIIILIWLYTHQSGIKTIVWTDTLQTIILITALILIIVEVSQQLNMGVKDIYRELVNY
                     PKTRIFVFDDWNDKQNFFKQFFSGIFIAIVMTGLDQDMMQKNLSCHNLQEAKKNMYWY
                     GFSFVPVNFLFLVLGALLLVFSAEKGIALPPLGDEILPMLAANYLGQTVMIFFITGII
                     AAAFSSADSALTALTTSFSVDILGIQHQESRKANHTRRIIHVCISIFFAIIIVIFKAI
                     NNKSVIDAIYTVATYTYGPLLGMYTFGLFTKMQVKDKFMPYIAVLSPILCGLMDYLFT
                     RYLHYSFGYEILIINGLLTFTGMWIFRKKNIIFNTIKS"
     gene            28787..29392
                     /gene="yihA"
                     /locus_tag="PLZ54_01785"
     CDS             28787..29392
                     /gene="yihA"
                     /locus_tag="PLZ54_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOJ65703.1"
                     /translation="MEIHATEFIKSSTDYKGCPEGNFPEFAFIGRSNVGKSSLINMLC
                     QRKNLALTSSKPGKTLLINHFLINKNWYLVDLPGYGFAQTGKENRKKLIDNINNYILF
                     REQLTCLFVLIDVRHEPQKIDLEFMEWLGSNGIPFCIVFTKTDKLSASKIDENIENYK
                     QKMLENWEQLPPFFKTSSHKKIGREEILNFIDKIIESLKVQ"
     gene            complement(29434..29889)
                     /gene="smpB"
                     /locus_tag="PLZ54_01790"
     CDS             complement(29434..29889)
                     /gene="smpB"
                     /locus_tag="PLZ54_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HOJ65704.1"
                     /translation="MHKTSNIVIKNKRATFEYEILDRYISGIQLFGTEIKSIRSGKAS
                     LSDTYCTFINDELWVKNMHIAPYFFGSYNNHETRRDRKLLLKAQELKRLQRAVKESGN
                     TIVPLKLFINERGLAKLEIALVKGKKLYDKRQTLKEKEDKRYIERTLKR"
     gene            30103..31263
                     /locus_tag="PLZ54_01795"
     CDS             30103..31263
                     /locus_tag="PLZ54_01795"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HOJ65705.1"
                     /translation="MTQQELKSAFEKIYGQPADAVYFAPGRVNLIGEHTDYNGGYVFP
                     CALSFGTYLLIRKNGGNTVRFHSLNQPNAVEVPLEKLTTRLENSWANYLIGVFAQFIK
                     KGVKIDEGFDILIWGDVPTGAGLSSSASLEVVTAYALNDQLGTGYDRTELALMSQKAE
                     HEFALVNCGIMDQFAAAQGKKDHAIFLNCDTLEFDLVPVKLNGIKVLISNTHSPHKLD
                     SGAYNQRVAECKKAVEQLNKVRSIKYLAELTEEEFKKIESAITDETAKKRARHVVSEV
                     QRTKDAVKALRAGDILQFGKLMNASHVSLRDDYEVTGPELDTMVEEAWKIDGVIGSRM
                     TGGGFGGCTVSLVKDEAIDTFIKNVGAAYEAKIGIKPEFYIAEIGDGACRLS"
     gene            31349..32389
                     /locus_tag="PLZ54_01800"
     CDS             31349..32389
                     /locus_tag="PLZ54_01800"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOJ65706.1"
                     /translation="MKSEESSQITVHGKTVKLFTLTNKNGMSCSITNFGAKVVKLFAA
                     DKEGKFEDVVLGFDTLNECIEREPYFGAVCGRFANRIKDGKFVLDGVEYHLPINNGTN
                     SLHGGIHGFNEKIWEVKSVDNQEIIMEYLSVDGEEGYPGNLTVKVTYFLSDNNELKIH
                     YEAVTDKATVINLTNHSYFNLKGAGNGTIRNHILQLNADFYTVLDDSFAPTGEIRPVE
                     NSAFDFRQPTVIEQRIDDEAFAPGRGLDNNWAIRKHHTGEMVVAGFLYEPESGRKMEV
                     MTTQPGVQVYSGNWIDKQTGKNGKVYESQHAICLETQGFPNSANVPFFPTPVLRPGQK
                     YDEWCIYRFSAE"
     gene            32566..32712
                     /locus_tag="PLZ54_01805"
     CDS             32566..32712
                     /locus_tag="PLZ54_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65707.1"
                     /translation="MSTYEQLEALARYILPKEILDDFDIVGIEPKLLISQFKGKVKLT
                     RTVP"
     gene            complement(33187..33375)
                     /locus_tag="PLZ54_01810"
     CDS             complement(33187..33375)
                     /locus_tag="PLZ54_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65708.1"
                     /translation="MGKISVHFAAAQLNRVVEISKVKHLPNQIIKSSLTFLFNNFVIK
                     RLRKNLEQLRKDLQWLTA"
     gene            33731..34147
                     /locus_tag="PLZ54_01815"
     CDS             33731..34147
                     /locus_tag="PLZ54_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65709.1"
                     /translation="MKKILILMVSALFLISVAGHAQNQKNVSDKEGKKSEMRQNASQR
                     MSAESRAEYWAKELNLTNEEKAKIAELFKKQDETRMQHQAEMQKSRDEHRAFMESQRK
                     ANDAEMEKIIGKEKFQKYDSIRMARMQTMRNRMRNR"
     gene            34522..36099
                     /locus_tag="PLZ54_01820"
     CDS             34522..36099
                     /locus_tag="PLZ54_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOJ65710.1"
                     /translation="MSFQEEIRRRRTFAIISHPDAGKTTLTEKLLLFGGAIHVAGAVK
                     SNKIRKTATSDWMEIEKQRGISVATSVMGFDYQDYKINILDTPGHQDFAEDTYRTLTA
                     VDSVIIVIDNAKGVEEQTRKLMNVCRMRKTPVMIFVNKMDREGKDPFDLLDELESELD
                     IHVRPLSWPINQGFSFKGVYNIYEENLQLYTPNKQTISESVEISDIDSEELEKFIGEK
                     DARQLRQDLELILGIYPAFDREKYLSGELAPVFFGSALNNFGVKELLNCFVEIAPSPQ
                     PVQAHERVVNPYEETFSGFVFKIHANMDPNHRSCIAFVKICSGKFERNVNYKHIRQGK
                     MLRFSSPTAFMAQKKEIVDEAYSGDIVGLPDNGNFKIGDTLTGGEDLHFKGLPSFSPE
                     MFNYLENADPMKTKQLEKGIRQLMDEGVAQLFTSEFNRRKIVGTVGQLQFEVIQYRLL
                     HEYNAQCRWEPISLYKACWIESDDAEQLEAFKKRKAQFMVKDKEGRDVYLAESPYILQ
                     MAQRDFDHIKFHFSSEF"
     gene            complement(36141..36449)
                     /locus_tag="PLZ54_01825"
     CDS             complement(36141..36449)
                     /locus_tag="PLZ54_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOJ65711.1"
                     /translation="MENNENQINIELSAEVAEGIYSNLAIISHSSSEFVLDFIRIMPG
                     LPKAPVKSRIILTPEHAKRLLYALQENIAKYEQQNGKIKIDENSFIPPFPMGFGGGEA
                     "
     gene            complement(36591..37352)
                     /locus_tag="PLZ54_01830"
     CDS             complement(36591..37352)
                     /locus_tag="PLZ54_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HOJ65712.1"
                     /translation="MRTTKFILFSFICISLTSCNVLMQGVSNSGTLSIPTTDKENIAG
                     IKNALLLGTERAVSDLSIENGFYGNQAFKLLLPEEAQTIVENISLIPGGKALVDKAIL
                     SINRSAEDAVKEAGPVFKKAIQDMTISDAMGILLGGSNAATQYLQKTTSTELKNLFLP
                     KVQASLKKPLVGDVSTTETWNLLINAYNSAANSTLGKLASLKPVNTQLDQYVTDKALS
                     ALFTKMAETENSIRTDPAARTTTLLKRVFGQLDNK"
     gene            37523..38812
                     /gene="eno"
                     /locus_tag="PLZ54_01835"
     CDS             37523..38812
                     /gene="eno"
                     /locus_tag="PLZ54_01835"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOJ65713.1"
                     /translation="MSRIEKIVAREVLDSRGNPTVEVDVMLESGVMGRAAVPSGASTG
                     ENEAIELRDGDKKRYLGKGVLKAVSNVNNIIAPSLIGISALDQRTIDKKMIELDGTKT
                     KSNLGANAILGVSLATAKAAAAYLDLPLYRYIGGVNAFVLPIPMMNIINGGSHSDAPI
                     AFQEFMIRPVGAPSFHEGLRMGAEVFHSLKKVLHDRNLSTAVGDEGGFAPTLKGTEDA
                     LESIITAIEKAGYKPGQDVMIGLDCASSEFYENGIYNYTKFEGANGAKRTSEEQVKYL
                     SELVSKYPIDSIEDGMSENDWEGWKLLTEKLGNKVQLVGDDLFVTNVEFLKKGIEMGC
                     ANSILIKVNQIGTLSETLDAIEMAHRAGYTTVTSHRSGETEDATIADIAVATNSGQIK
                     TGSLSRSDRMAKYNQLLRIEEELGDLAVYGYKKVTKK"
     gene            39074..>39401
                     /locus_tag="PLZ54_01840"
     CDS             39074..>39401
                     /locus_tag="PLZ54_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease HsdR"
                     /protein_id="HOJ65714.1"
                     /translation="MEASKRKKALGLFLLVVVVSVATNVTTNYFTNKHEKDNFKRELQ
                     YPTAYAKFSNVKSGVETDFTLAAELTIHSVVHVKTQSKAKMAFREDPFFEFFFGRPRY
                     YQEPPMV"
BASE COUNT        12978 a         6900 c         7405 g        12118 t
ORIGIN      
        1 ctcttttctg attattcacc gaaaacatag attttatatt tagcaaacaa tcctaagata
       61 tttgtagatt gttcatctac atgatgaatt ttggttatac cagcagattt tgcagctgct
      121 tcaatgctgg catctcctgt tgccacaaga cccaaaatcg aagttgcttc tgctgttcca
      181 acttttgttg aacttgaatt agaagttgca gttaaaggag ctttaacatc agtatacaaa
      241 acagcaccta aaggactttt tactatagca caacttgaaa agaaaaatac aatacctcct
      301 aagatcaaaa tttttttaat ttttttcata aacatagttt ttttagttat acctacttca
      361 gatttttaga gttgactttt cagcttattc tcaacttttt tattctactt atggcgtcag
      421 aaagccattt ttacgttcat tctcttaatc aactttttta aggccatttc attaccttgt
      481 ttctttccat caattttggg tttactttac cataacccgg acaaatttat acaataattt
      541 caattattca aaacaaatga ttaaaaaaat taatacatta ttttgggtaa tttataaaaa
      601 aaggaaaggg cgatcgtggt tttcgtggct atagaacaac ttttctgagt ttaatttttc
      661 tttaaattgg ctaatgagta attcgggtta aaataaagaa gattgagtat cttttttggg
      721 atgaaatgtt cctataatca taaatggatt gtgagactta aaatgatgta cttgcgaggt
      781 taccaaaaag aaatgcaagt cttttgtttt tgcaaagtca tcgaaatact tcatcttaac
      841 agcttccccc accgctctcg ccatttgcaa tcggtggttt ggtctgtaga gcaagaaata
      901 aagagcaaat gtaaaaatga atgcgatcgc ataaaaaaac aaaaaatttt tacatttgtt
      961 ttatatatct gtaaaacaat acatataagc caagctcttc gggttgattt tgtccgatga
     1021 gccaaaaaat cagagaaatt ttcgttaaaa aatcttttct gtttgagctc cgcaaggagc
     1081 gagtttagaa gattttagcc aatgaatgtc catttttctt gtaagtgggg tttggggcaa
     1141 agctgcgtta ttaaatatct gaaaataaag aatataaatg tttctacaat tttttgtcat
     1201 gtagtaaggt aagaaacatt aaacaatatc gagcctgtat ttgttgtaag ttgaaaattg
     1261 aataatatca aattattttc caaaaaatta ttaataatct aatttacaaa acattatggc
     1321 tttacaggtg ggagataaaa ttcctgaatt tttgggtatt gatcaggata acaaagaagt
     1381 acgccgttcc gattttaacg gtaaaaaact gattctttat ttttatccaa aagacaatac
     1441 ttcgggttgc acggcagaag cttgcagttt tcgagacaat ttcgatattt tacgggaagc
     1501 cggctacgag gtggtcggtg ttagtgccga tagtgtaaat tctcatcaaa aatttattgg
     1561 caaacatcag ctgcctttcc gattgattgc cgataccgac aaaaaacttt cggaacagta
     1621 tggcacttgg ggagaaaaaa cgatgtacgg gaaaaaatac tttggcatgt tgcgcacaac
     1681 tttcataatt gatgaagagg gtattatcag aaaaataatt ccgcccaaag aaatcaatac
     1741 caaagaacat gcccaacaaa tacttgaact tgtaaaaaac atgtaaagga aaagagcggt
     1801 tgaaattttt tctaaaatca accgctcttt ctacttttca actattgaaa acagaactta
     1861 agtagcagtc gagaacggat ttggccataa ttctgagaat attgttttta taggcatcca
     1921 gcaaatccgg ggacagctct tttttctcta aattgtttaa gcggattttg tccttgttgg
     1981 cttcgagcat ttggataatt tcatagcagg acaaatttct gctgttgagt tcgcttatcc
     2041 aattatcgat atttggttga aattgagaaa taatcaacat tgcatcatcc cggatagcat
     2101 cgcggtatgt tttcggagtg gaggggacaa aattgttgat ttcgcgcttg tactgaagta
     2161 aaactttgtg gtaattttcg ccggccataa tttttatatt ttaggtattt gaaagtcaat
     2221 atattaaatt attcccccat caatatttca acacttgaaa tattctattt cattccgtta
     2281 ttattgtaac aatcgaaccc tgaaaaagtt ttaagcaaat aatgttccaa atcaaaaata
     2341 tcctaattat ttaaaaatcc ttcacacgtc ggaattttgt agtaaaaaaa tcacatatat
     2401 ttgcaccgat aatttcagta tttctatttt atacgaattg ctacttagaa ggatttaaca
     2461 tgtcggatat atatcgaacc attgctacaa gagcagaagg aatgtataag gacaaaggaa
     2521 gcaaatttct ttcttttgcc cttccggtgg aaaacgtgga agaaataaaa aaaatcataa
     2581 aggatttcca gcatcagtac catgatgcac gccatgtttg ttatgcttat gtattgggaa
     2641 acgaaaaaaa ggactggaga gcaaatgatg ccggtgagcc ttcgggaaca gcaggcaggc
     2701 caatattgag gcaaattaat gcccataatt tgaccaatgt gctggtaatt gtggtaagat
     2761 attttggagg cattcttttg ggcacgagcg gattgataaa tgcttactcc gaagcggctt
     2821 ccgatgccct gaatcatgcc gaaattattg aaaaaactgt tcaatgccat ttgaaaattt
     2881 cttttgattt tttacaattg aacagcatta tgaaaattat caaggaacaa aagcttcaag
     2941 tagttgttca ggaaattgac aatgaaaaat gttctattga attagctgtt gcacagcatg
     3001 aagtttccga tttgataaaa ataattcaaa atatctcggg gacagaaatt gaagttttaa
     3061 acaggtaaat aatctaaata cattttattg cacacagttt taataaaaac ttaagtggtt
     3121 ttttctcttt aaaactattc attgcaaaat cctcgttaaa aatgagtatt tattctcaga
     3181 aagtcattat ctttgcaatt gaaatgaaaa caactttttt aaaatatttt tttgaattca
     3241 attacttgcc ggttgtaagt aatgagtgga aaagaatgaa gtaaaccaat ataaatccga
     3301 cttcgatttg tattggtttt tttgttttta aagaattttt tcagaaaaaa acgcatacat
     3361 tttttgagga taggagcagg tgatagtttg tatttctcac aatgtttcaa aaaagatcca
     3421 ataagaaaat taaaacatat tgaatatgag caatagaagc ttagtttcaa tttcagacta
     3481 taacaaggag gaaattcttg aaattctgga tgctgctgcc gattttgaag ccaatcccaa
     3541 tcgtagcacc ttgaagggga aagtgattgc tactttattt tttgaacctt ccacccgcac
     3601 aaggcttagt tttgaaacgg cagttttaag gctgggcggt tctgtcattg gtttttccga
     3661 tgcggctact agcagttcgg tgaaaggtga aaccctcaat gataccattc gaatggtaag
     3721 ttgctatgcc gatgccattg tcatgcgaca tccttttgaa ggagcagcgc gttatgctgc
     3781 cgaaatttct cctgtcccta ttatcaatgc cggtgatgga gcaaatcagc atccttccca
     3841 gaccttgctt gatttgtatt ctattttgaa aacgcaggga acgctcgaaa atctgaaaat
     3901 ttgtgttgcc ggcgatttga aatatggacg taccgttcat tctttgatta tggccatgtc
     3961 gtatttccac cctacatttc ggtttattgc gccggaagaa ttgaaattaa acgaggaata
     4021 tagaattttt tgcctcatga acaatattcc ttttgaagaa acgacagaac ttaacgacaa
     4081 tatcaacgat gcagatattt tgtatatgac ccgtgttcaa cgcgaaagat ttcaagattt
     4141 gatggaatat gaaagagtaa aaaatgttta caccctgaaa aattccatgt tggaaaacac
     4201 caagcccaat ttgcgaattt tgcatcctct gcctcgtgtg tcggaaattg cgcccgacgt
     4261 ggataaaaat gaaaaagcct attattttca gcaggctcaa aatggattgt acatcagaca
     4321 agccattatc agcaaagttt tgtcggtcta agttttgtta gccgattttt tacgattcgg
     4381 aatcaaaaat ttttattttg aattttcaac tttatataaa gcatcatgga aaaagagctc
     4441 aaagtggcag cattgcgtaa tggaacggtt attgaccata ttccggccga taaattatat
     4501 aaggtggtat tcatgttgca tttggatact tgcgacaatc aaattacgat tggtaacaac
     4561 ctggaaagtg ccaaaatagg gaaaaaaggg attattaaaa tatccggaaa agtctttgca
     4621 gaagacgaaa ccaataaaat tgcactgata gctccaaatg ccaaaataaa tattatcaaa
     4681 gattttcagg ttatcgaaaa acgtccactt tcgttgcctg atgaaattca cgaaattgtg
     4741 cagtgtaata atcccaattg tattaccaac catcaaaata tccctacgtt tttccaagta
     4801 atcaaagatg aaaatgatac aatgcttaaa tgtcattatt gtgaaagact tatcaaattg
     4861 gaggaagtaa aaatcaaata aatttttctg tgttgaaaga cagttcgaaa aaattaaaag
     4921 ttatttatgg ttttatccaa acatttctgc gatgacaaaa actgacgaaa attcttctca
     4981 tgtttcatgg aaaccgggta caatgatata tccgctccct gctgttctcg tttcgtgcgg
     5041 gaaatcggcc gatgaatata atttgctgac agttagttgg gtgggaacaa tttgtacaga
     5101 gcctcccatg tgttatattt ccatacgtcc cgaacggcat tcttatgaaa ttataaaaag
     5161 aaatggcgag tttgtgctca atcttactac ggaaaaactg gcttatgcta ccgactggtg
     5221 cggtgtaaaa agtggaaaag atcatcgcaa gtttgaagag atgaaactta cacccgaaaa
     5281 ggggaaaatg gttgatgcac ccattgtcaa agaatctccc gtatgtatcg aatgtaaggt
     5341 taaagaaatt gttccgcttg gaacgcatca catgtttatt gccgaagtag ttaacattca
     5401 ggccaatcca cagtatattg acagcaaaac gggagccatg cacttggaaa atgcacattt
     5461 gatagcttat tcgcacggac attattacaa acttggagaa gaaatcggaa aatttggttg
     5521 gtcggtaagg aaacccagca aaaaaaagaa agtttcccca aaaattccaa aaaaatagaa
     5581 aaaactttat caacatctca gaatttgttt ggttaaaaga acgaaaaaca acaagtgtaa
     5641 ttcatatttt ttaaaataat aaagaacaga aaatgaaaag agaccaggaa atttttgaca
     5701 ttatcgaacg cgaaaaacag cgacagatga aaggcattga gttgatagca tcggaaaatt
     5761 ttgtgagcaa tcaggtgctg gaagcaatgg gatcggtttt gaccaacaaa tatgctgagg
     5821 gttatccggg aaaacgatat tatggcggtt gcgaagtggt ggacgaatcg gaacagctgg
     5881 ctatcgaccg attaaaaaaa atctttgatg ccgaatgggt gaacgtacag ccacattcag
     5941 gtgcgcaagc caatgctgcc gtatttttag cctgccttaa tccgggagat aaatttttag
     6001 gtttaaatct tgctcacgga ggccatttgt cacatggttc acccgtcaac agttcgggaa
     6061 ttttgtatca acccttgcaa tacaacgtaa aagccgatac gggaagagtc gattatgacc
     6121 aaatggaaga aattgcgcta cgcgaaaaac caaaattgat tattggtgga gcttcagcct
     6181 attcgcgaga atgggattat aaaagaatgc gcgagattgc cgacaaaatt ggtgctattt
     6241 tgatgattga catggctcat cctgcagggc tgatagctgc cggattactt gaaaatcctt
     6301 taaaatgggc tcacattgta acttctacca ctcataagac cttgcgtgga cctcgtggag
     6361 gaattatttt gatgggaaaa gattttccaa atccttgggg aaaaacgacc cccaaaggag
     6421 aagtaaaaat gatgtcggct ttgctcgatt ctgcagtttt tcccggtatt cagggtggtc
     6481 cgctcgaaca tgttattgcg gccaaagctg tggctttcag tgaatgtttg caaccggaat
     6541 ttttcgaata tcagaaacag gttaaaaaga atgcggaaaa gctggccaat gaacttattt
     6601 ccagaggttt caatatcgtt tctggtggta cggacaatca ttgtatgctg gttgatttaa
     6661 ggagcaaatt ccccgatttg acaggcaaaa tggctgaaaa tgctttggta aaagccgata
     6721 taacggtcaa caaaaatatg gttcctttcg atagtcgatc tccattccaa acttccggta
     6781 ttcgacttgg tactcctgcc ataacaacgc gtggagccaa agaagatttg atgactgaaa
     6841 ttgcagaaat gattgaaacc gttttacaaa atgcagataa cgaaaaaatt atccttcagg
     6901 taaaggagaa agttaataag atgatgaagg attttccttt gtttgcctac taaaagtgca
     6961 taaaaataag gaattttttc aaaatactgt ttgaatttta aaaatgtttt gatttggtaa
     7021 aaagaaaaat caatatcttt gcaggatttt gaatattaat tttgtaatta ttttgcagcg
     7081 tgggaattaa cgttaaaggg aaaatagcca ttattggcgg aggaagttgg gctacagctc
     7141 tggctaaaat tgctttgagc aacgaacagc aaatcaattg gtatatgcgc cgacaggagc
     7201 agatagatga atttattcgc caaagccaca atccatccta tcttaccggt gttgaattcg
     7261 atacagaccg aattaatttt accagcgaca taaacaaagt ggtaaaatcg tccgatgtgt
     7321 tgatttttgc tactccatct ccttttttta agggacattt gaaaaaattg cgtcaaaagc
     7381 tggacaaaaa attcgttgtt tcagccatca agggaattgt gccggaagaa aacatggtta
     7441 tttcggaata ctttgagcaa ttttacaatg tttcgcccga aaatatggcc gttttggccg
     7501 gtccgtgtca tgctgaagaa gtggcaatgg aaagactttc ctatctgact gttgcttgcg
     7561 cttcaagaga aaaagcccgt atgctggccg ataggttggc taccaatttt attaaaactt
     7621 cggttagcga cgatgtgttg ggaattgagt atgcttccgt catgaaaaat atttatgcca
     7681 ttgctgcggg tatttgtcac ggattaaaat atggcgacaa ttttcaggcc gttctcgtct
     7741 cgaatgccat tcgtgaaatg aatcgattct gcaatgccat gaacgctcct catcgcgata
     7801 tttccgagtc ggcttatctg ggtgatttgt tggttacgtc ttattcaaaa ttcagcagaa
     7861 accgtctgtt tggtaccatg attggaaaag gttattccgt aaaaacggca caaatggaaa
     7921 tggaaatgat tgctgaaggt tattatgcca ccaaatgtat tcacgaaatt aacgaaaaag
     7981 ttcaaatcag catgcctata gtcgatacgg tttttcaaat cctgtataat cgttgttctc
     8041 ccattatcga aattcgtaaa ctgacagaac aattaatgta aataacaaac aaaattttta
     8101 ataaaaatgg aacatataca attttttagc gacaagtctt cagtaagcaa agaaaaaatc
     8161 gctgaatata aagagaaagc cattcggtgc atttcggcac ttcatgaagg aacgggaaaa
     8221 ggaagcgatt ttttgggttg gctgcatctt ccgagcagta ttgatgaagc tcaattcgct
     8281 gaaatagaaa ataccgccaa acttttgcag aaaagttgtg aagtagtcgt agtaatcgga
     8341 ataggtggaa gttatctcgg cgcaaaagcg gtgattgatg ccttgtcgaa tagtttggac
     8401 tggttgcaga aagttcgcaa atcacccatt attgtttatg cgggtcataa tatcagtgaa
     8461 gattatttgt ttgaacttca ggaatttctc aaagacaaaa catttggcat catcagtatt
     8521 tccaaatcgg gcactactac tgaaccggca ttggctttcc gccttctcaa aactcaattg
     8581 gaagaacagc agggaaaagc cgaagcaaaa aaactgattg ttgccattac ggaccgttct
     8641 aaaggtgcgt tgcgtaaatt agccgaacag gaaggttata aaacctttgt catagaagac
     8701 aacattggcg gacgttattc tgtgctgaca cctgtaggcc tgcttcccat cgcaactgcc
     8761 ggcttcgaca ttcgtcagct tgttgcagga gcagccttta tggaatcgct ttgtggaatg
     8821 gatacgccat ttgaaaagaa tcccgctgca caatatgctg tggtgagaaa tgagctttat
     8881 cagcaaaaag gcaaaaaaat tgaaattctt gtcaattttc atcctaaatt gcactatgtt
     8941 tccgaatggt ggaaacaact ttatggtgaa agtgaaggaa aagatcacaa gggaattttt
     9001 ccggctgcag ttgattttac aaccgacctg cattctatgg gacaatggat acaggaaggc
     9061 gaaagaagta tttttgaaac ggttatttct attaaagagc ccaatcattt aaaaactgtt
     9121 ccttttgatc ctgaaaattt agatggtttg aattatttgt cggggaaacg ggtagacgaa
     9181 gtcaacaaaa tggcagaact tggcaccatg atcgctcatg ttgatggagg agttccaaac
     9241 ttgaaagtgg aaatgccgaa gttgaacgag tattatttgg gtgaattgct ttatttcttt
     9301 gaaaaggctt gcggtatcag cggttatttg cttggtgtaa atccttttga tcaacccgga
     9361 gtggaagctt ataagaaaaa tatgtttgct ttgctcgaaa agcctggata tgaacaggaa
     9421 acgaaagcca taaaagaaaa attgaaataa gtattggatt tcaatcattt atacagttaa
     9481 ggctgcttca acgaggcagc cttaactgta taaattaaaa tgatttgtct attacgaaaa
     9541 ttttatacaa aaatatattc atccttgaat gttataattt tccgtgttcg attaataaag
     9601 ttttggttgg taaatcacac acttctgttg gaccaaaata ttgaataggt ccgggataaa
     9661 tgtagcttac cttttcgtca gcccattcat ctcgatgggc ttccaaatat ttaaaaggtg
     9721 caccatcaag ctttactaat gccttttgta tcacgggttt cattttttga ttccttttct
     9781 ccatattcat caacatagtt aaaggcacac cacaagcaat ccaatttgca ggttcatctg
     9841 tcaaattttt cactgaagcc atataaccgg tttttccttc agcaattaaa agtgaggcaa
     9901 caattcctaa tgtataggtg tagttggcgt caaaattgga tgggatggca caacgtcctt
     9961 cataaccaaa gaaatgactg atagcagaaa agattccctt ataaacacct tccgctttca
    10021 attgagccaa acgttttgcc accatttcta tcaataattt ttcagtttca atgagcgaca
    10081 cttgaacatt tccatgagga tcgcgatcca tagaaagctg gcgtgcaatt ccttttggta
    10141 aacttctgta aagttgactt gcttcagggc tcaacatacc cttgatatat tgacgttttt
    10201 catcatcggt atgaagagct acaaaatctt cattattagc caacaaatcg tttaattcct
    10261 gaataagttt tttcattccg ggaataaact caatcaatcc ttcagggata agtactgttc
    10321 catagttgat accatgattg gctcgttgaa cgataatttg aacaatatat tccacaatat
    10381 cgcttaaact cagatttttc ccttcaactt cttcggaaat aagtgttata ttcggctgac
    10441 tttgaagggc acattccagt gtgatatgcg aagccgaacg acccatcaga cgaataaaat
    10501 gccaatattt ttttgcagag ctggcatctc gttgaatatt tccaataagt tccgaataaa
    10561 ctttacatgc tgtgtcaaaa ccaaaagatg tttcaatcat ctcgtttttc aaatcaccat
    10621 ctatcgtttt agggcaacct attacctgaa taccaaaatt tttccgtgcg taatactcag
    10681 ccaacacgca agcattggtg ttagaatcat ctcctccgat gatgactatc gcttttattt
    10741 ttaactttct gcaaatctcg gctccttgtt caaattgcca ctccagttct aattttgttc
    10801 ttcctgaacc tataatatca aatcctccag tattacgata ttcatcgata atttccgaag
    10861 tcagttcagt atatttatga tcaaccagac cacttggacc atccaaaaaa ccataaagtt
    10921 tatttttagg atttaatttt tttaatccgt caaagagtcc tgcaattaca ttatgtccac
    10981 ccggagcctg cccgcccgac aaaataacac ccacagcaat ttcgggatat tcaacctgtg
    11041 ccccttcttc aaaagtaata atcggtaatc cgtaagtttt aggaaataaa ttctttattt
    11101 ctttctggtc agcaaccgat tcggtaggtt cgccatattt taatgcaatg tttccactca
    11161 gaatagtagg tattttgggt tgataacttg aacgtgcaat ctgcaaggga ctcttattgg
    11221 tttccataaa aaatttttta aagggttgat atgtaaatgt aagtcttttt gaaaatctat
    11281 ttgcaaaatt aatcataaaa tagaaaaaca acaagcaaaa agcattcaat tttcaataag
    11341 cacaaatata atttcaactt ttaaatatca ttttcgatgg tgaaaagatt aaattttgag
    11401 taaaaacaca aactattcat ttgcaatttt ttgttaaata aattattgag ttttcaatca
    11461 actctgctaa attgaaagat tatcactatt tttgtagctc tattctgcct gaggagaaaa
    11521 tttatattca aaaaacacgt tatatcttta ctttttaata gtgtcttatc atattagtta
    11581 catcaaatta tgtgcaagaa ttcctttagg aataacgtgc tgattgtttt tatgattcta
    11641 cctcttgcat tacaagcgca agatcaatat ctggcagaaa ttggagtaac agccggcaac
    11701 ggatattatt tgggcgatgc gaacgaaatg ccttttaaga attccagttt ggtttatggt
    11761 tctgtttttc gctatagaat tgatccgcgt ttatccgtac gggtagaatg gaatcatggt
    11821 actgtagaag ggaaatattt taattctacg ggcgaaacat taaatttcgg caattcattc
    11881 aatgccgtag atataaacgg agaatttaat ttctttgatt taatgcaaaa atcaaatagc
    11941 cgtaacagca aaaaatattc aacatatatt tttgccggtt tgggaacaac gatttatgct
    12001 aacgaaagca tcaatacagt aacagctttc agtattccgt ttggagtcgg atttaaatat
    12061 aaagtcagca cacgtttcaa tgtaaattta cagtggaata tgcgttggtt aagtagtgat
    12121 gatttggaag cagttaatga tttgaacaat gcacaaagat taaatggttc caatccattg
    12181 aataaagatt ttttttcgac tgttacttta ggaatcactt ttgatttctt aaaaaaacct
    12241 tgcgattgca aatcgaaatg gtaaaaatga aaaccatcta tgtttaaaga aaaaattgat
    12301 tatacgcatc tgcccaaaca tatagctata ataatggatg gcaacggacg ttgggcaaaa
    12361 gaaaaaggat acaacagaat ttacggacat cagaaaggcg taacttcagt tcgtagaatt
    12421 acagaagcag ctgcagaatc taacattcag tttcttacgc tttatacttt ttccaccgag
    12481 aactggaatc gtcccagcga tgaaataaaa gcattgatgg aattgctggt aattactatt
    12541 gataaagaaa tggaagattt gaacaaaaac aacattcgaa tgctcaccat tggcgatttg
    12601 tcgaaattac caaacgatgc cgcagaaaaa atccgtgaat gtaaagctaa aacagctcaa
    12661 aatacgggac tcaccttgaa tctggcatta agttattctt cccgatggga aattaccgaa
    12721 gccgtaaaaa atattgtttg cgatgtagaa aaagggaaac taacttcaaa aaatatttcc
    12781 gacaaactaa tttcctctta tttgtccact gccggcattc ccgatccaga cttaatgata
    12841 agaacaggag gtgaactaag aatcagcaac ttcttgttat ggcaatcggc atataccgaa
    12901 ttctatttta cttccaaata ttggcctgaa ttcgataaag aagattttta cgaagctata
    12961 tttgattttc agcaacggga acgtcgtttt ggtagataaa taaatgatat ataaaaaatt
    13021 gactgattga atttttatga aaaataaaat ttattttatt ctattcttga tatttttcca
    13081 tcaactctct tctttttcgc aaaattcgaa aaacgattcg acctctattg caactgaaaa
    13141 tgaatcagta ccaatgccgg ttattgagta ttcaaataca aatcccaaat atgaaattgc
    13201 agaaattacg gtcactgggg ccgaccacta cgaagatttc gtattgattg ggttttccgg
    13261 tttatctgta gggcaagtga taagcgttcc gggaccggaa attaccgaag ccgtaaaacg
    13321 attttggaaa cagggtcttt tttctgatgt taaaatctat gccaccaaaa tagaaaatgg
    13381 aaaagtatgg ttgaatattt cattgaaaga gcgtcccaga atatcaaaaa tcaattattc
    13441 cggtttaaaa aaaggtgaaa tagaagattt ggaaaaaaaa ttaaatttat ctgtcggcaa
    13501 tcaaattaca ccaaatatta cggaccgaat aattacggtt ataaaaaaat acatggaaga
    13561 aaaaggtttc ttaaacactg acgtttttgt ttaccaaaaa gacgatcccg aaaaaccggg
    13621 acacgtgatt gtggatatcg aagtgaacaa gaaaattaaa accagtgtcc ataagatata
    13681 tgtaacagga aatcaagctt tgacagttgc acaaattgac aaggccatga aaaaaaccaa
    13741 cgaaaaaaat ttacgcaact tttttcgcag caaaaaattc gtaaaacaag aatacgaaaa
    13801 cgacaaaaaa ctaattatag aaaaatataa cgaaatcggt tatcgggacg cctatattgt
    13861 tacggatagt attgttccct ttaacgaaaa aagcgttgac atttatctca ccatcaatga
    13921 aggtaaaaaa tattatttca gaaatatcag ttggataggt aacacagttt atccttacga
    13981 gtttttaaac gagaaactag gaataaaaaa gggagacctt tacaattaca attatttaat
    14041 caagcgcctt ctaacagacg aaaacgatgc cataagcaaa ttatatcaga ataacggcta
    14101 tctgtttttt caaattgaac cggtagaagt acatgtagaa aacgattcta ttgattttga
    14161 aattagaatt tatgaaggta aacaagctac catcaacgaa attggtataa aaggaaacac
    14221 ccgcgtttac gaacatgtga taagaagaga attgcgaact aaacccggac aattgtacag
    14281 tcaggaggat atcgtccgtt ccttgcgtga actggcgcaa atgggtcatt tcgaccaaga
    14341 aaaaatgaat aatgccattg atattcaacc cgaccccgaa aatggcaccg ttgatattaa
    14401 ttatgtgctc gaaagcaagg gaaacgatca aatagaattt tctgccggtt ggggagcaac
    14461 aggagttgtt ggtagtgtag gattaaaatt cagtaatttt gccatccaaa atttatttaa
    14521 tcccgatact tatcgtattg taccacaggg agaaggtcaa actttttctt taaatgcccg
    14581 caccaacgga caatcttaca gttctttcag tatttcgttt cttgaaccat ggttgggagg
    14641 aaaacgcccc aattcacttt ctgcctcagt atattattct acacaaactg ccattagcaa
    14701 ccgatacctg aattacatca ataatctttc gagtatgtat tatggtggtt attacggagg
    14761 atattacaac gattactacg gcaataacta taattactat caaacggaag tagaccccga
    14821 ccgctatatg cggacactgg gggcatcctt aggctatgga aaaagattga attggcctga
    14881 cgattacttc tctttctatt ccgaactttc atttcaacgt ttcatgctga acaactggta
    14941 tgccggatat tttcctatga cttcgggtac cttcaacaat ctaagtatga atctgatatt
    15001 gaaccgttct tccatagata atcccattta tacccgtacc gggtcattgt tctcattagg
    15061 acttaaattt acgccccctt attctgtttt caatggcaaa gactattctc aagaaatgag
    15121 taacgaagaa cgatataaat ttatagaata tcacaaatgg acatttaccg gaaaaacttt
    15181 tacgccactt actcccaaca ataaattggt acttatggca agagcacagt tttcctattt
    15241 aggttattac aacaaaaatg cccgatcacc ttttgaaact ttctacatgg gcggcgacgg
    15301 aatgtcgtcc tacaccagct atggcaccga atatgtggcc atgcgtggtt acgaaaacgg
    15361 ttcactcaca ccttacgata ccaaaacagg gattgcagca ggttatttgt acaacaaata
    15421 tactatggaa ctgcgttatc cgctttcgtt ggaacagtct gctacaattt atgttttaag
    15481 ttttgttgaa gcaggaaact gtttcaacag tatttccggt cccaatggtt acaacccatt
    15541 taatctgaaa cgatcggcag gtataggtgt acgcatattt cttcctatgt tcggcatgat
    15601 gggtatagac tggggttatg gattcgatac ggttaccgga gcaactaaac caagcggcag
    15661 tcagttccat ttcgttcttg gacaggaatt ataatacatt agattttctg gcaaagaatt
    15721 tgttataaat taaaattatt aagattaaat ctttcgatta tgaaaaaatt gatagtaaca
    15781 ttctgttttt taagtgtatt cacatggtta tcttctgcac agaaatttgc ctcagtggat
    15841 atgagctata ttttgaaaaa tattccggca tacgaaactg ccaacgaaca aatcaatcag
    15901 gtttcaaaaa aatggcagtc ggaagtagaa gctcttcaga aagaagctga agccatgtat
    15961 aaaaattacc aaaccgagtt ggtatttatg acagaagaga tgaagaaaaa gcgtgaagag
    16021 gaaattgttg caaaagaaaa agaagcacag gaactgaaaa gaaaatactt cggcccgcaa
    16081 ggagaacttt tcaaaaaaag agaaagtttg atgaagccta ttcaagatga agtttacaca
    16141 gccattcaaa gcatcagcaa agatcgaaat atagaagtgg tttttgataa aagcacggcc
    16201 atgagtgtag tttttacaac acccggcatc gacattagcg atgaggtact caaacagttg
    16261 ggttattcaa agtaatttaa tatatttgca ctctgttttt caaatttgta taatcaacat
    16321 caaaaaaatt gtcatgttta agaaaattgc ctttttatta ttatgtgtct tccctatcag
    16381 tttaatgggt caagaagtaa aattaggaca tatcaattcg caggaaattc tttctttaat
    16441 gccggaaaga accgaaatcg aaaaaacttt aaacgatttg caaacgcaat gggaaaatga
    16501 actgttaaaa atgagggaag aatattacgc taaaatcaaa gaatttcagg acaaacaagc
    16561 caccatgccc gaaagcatca aacaggctcg ccaaagtgaa attgttgaaa tcgaacaacg
    16621 tatcaatacc ttcaaccaga ctgccagcag tgatttgcaa aaaaaacagc aggaattatt
    16681 tgcaccggta attgataagg taaaaaaagc aatcaatgaa gtgggtaccg aaaataatta
    16741 tacgtacatt tttgatttaa gcacacaatc cattgtttat caatcaccaa aatcgaatga
    16801 tgttactgct cttgtcaaaa agaaattggg attgaaataa tcgagaaaat tattacgcta
    16861 gattatttaa gcctttgatg accagctcat tgaaggctat ttttttaaca aataatgatt
    16921 taaaaaaagt atcggatggt aaaacagcat gcgaggacct gaaaatctca tcacctgacg
    16981 tatttctttt ctcatttgag gttcatagca atgtatttta caggacgaac aggaagtctt
    17041 tctttctcca aaaggacatt tttccaaccg gcttacagca tattccgaca actgcaaaca
    17101 agaatcacac aatgaagatg tatgatgaca tttgttacag tacaaccgaa tcatttgttg
    17161 tacaatctgt ttttcgccct caatcttgtt catttgaaag aaaaaagaaa ttgatcgaat
    17221 gaattttatt cgggaagttt atattcgtta tccggtaatg gtaagttgtt ttctgaaaga
    17281 ccgatattat tttcaggaat gctgattttt ctgaataccc aatgcttctg caaattataa
    17341 ttaaatccta tggatataat taaatcggta ataattcggc tgaatttata atcgatttta
    17401 agaaatgtag taatcaaata ggttgaagaa gattttaaaa caatactgtt ggtaaccacc
    17461 aaagcaaatt tgagcaattg gtgcattccg gtagatttat aagggagttc tttcgatttg
    17521 aaagtccagg ttttattcaa tgtaaaattt acgactgctc ccacaattcc accaatggcg
    17581 atggaaatgg tataatgaat atgaaaaact tcagtgaaaa aaaccatcac catgtagtcg
    17641 accgcaccac cggtcatggc agaaaactga gctttggtaa atgttttaag cctctccata
    17701 aggttattga gcaatttttg cttctttttc ttttttatct cgctcatctg cagattttaa
    17761 tattttaccc gtttctaaaa aattcatgat caacaggacc aagcaagcaa gagctgatgt
    17821 atataaaatg gaatccttga caattacttc aagaataagc attaacgaaa tgagaatgcg
    17881 tacttctgta ggaccaaaac ttcccgagtc aattgaatac tgctctgtaa ttttatatct
    17941 gagtaaagcg gttatcattt cccaaccata aagaactaca aacagaaatc cgattatttt
    18001 ccaataagaa ggcgcataaa cgataaagcc ggttcccatt aagataattc ccagccagtc
    18061 gacagttata tcgagtgaaa aaccgtacca cttacgaggc ttgttgcgat agtatgctat
    18121 tctgccgtcc agcgaatcgc caaaccaact taccataaat cccaatatgc ccaacaacaa
    18181 aaagtaacga ctaatgaaag cacccagtac aaaagacaaa aacacaatca cgctccccac
    18241 aaaacctatc gctgtaagta tatcggaact catccaagcc ggaatttttt gtaccaaata
    18301 agcaatcaat ttttgttcaa cactgtgaag aatattggtt cgaactcttc cctgggcaat
    18361 taaactctcg gctctttttg tctcttgatc catgcatcaa aattataatt atctgattga
    18421 gaaggacatg ttgaattatt aaataaaaat ccaacacatt tttctaaatt cttattctgc
    18481 aaagttacaa atttttttaa aacaagtacg taacggcgat tgtttttgaa aatattttca
    18541 aaaaaacaaa gaaaacaaaa ttcttataaa aaaacaaaaa atcagataat tttctccaaa
    18601 gaaagtagga caggaagaaa aactaatccc caaaaaattg ctattgaatt taaatctcta
    18661 ctcgatttga ttagttaaaa tttatatctt tgcatctcaa attcctgaaa ttattagttc
    18721 tattaaagat taaaaaatga acaggaaaaa gaataaacga atttttggat atccacgaaa
    18781 tatttttttt accggattaa ccagttttct cacggacact tcactaaaga tggtctattc
    18841 ggtcatgccc atgtttttgc tttccattgg ggcatcaaaa accagtttat cgcttattga
    18901 aggagttgct gaaagtactg cttcactgat caaaacaatt acgggttttt ggagcgacaa
    18961 aataggcaaa aacaaaccat ttttactgat tggctatgga gtttcggctg ttattatacc
    19021 tttgtactcg ctggttgttt cacccattca ggtgctgatt cttcgtttcg tggaaagagt
    19081 ggggaaaggc attcgcacag ctccacgcga cagcattgta gccggatcgg tagaaaatgg
    19141 cgaaacagga aaaagtttcg gactgcaaaa agctatggac aacagcggag ccattttagg
    19201 cccactcata gcatttatct tgctttcggc attcaaagac aattacagat tgattttttt
    19261 gctcgccggc attccttcca tattgggcat tttggtgata attttcggca taacggaggc
    19321 taagaaaaat aaggaaagtt tgttgaaaaa atttcgtttt ggcgattttt caaaaaaatt
    19381 ttatttcttt ctggctatag tttttatttt tacgcttgga aattcaaccg atgccttgct
    19441 gctggtaaaa gccaacgaaa taggaataaa agttgccttc attccgcttg tttatttact
    19501 caccaacgtg gtttccgtat tgctgtctat tccagttggc agtttatccg acagagtggg
    19561 gaaagaaaaa attttgattg tcgggtatat aatatatgct ttggtttatt ttggttttgg
    19621 agcttccagt catatcgtgg taattgcttc actctttgcc ttatatggag tctattctgc
    19681 tgccactgat ggcatacaga aagcatttgt ttcggacatt gttgatgaga ataaaaaagg
    19741 aacgggtttg gggctctaca atgcattatt gggaataacc ttgctacctg ccagccttat
    19801 agccggcata ctgtacgaca aagtaaattc acatgttcct ttttatttcg gagcagtaac
    19861 tgctttaatt gctgctgttt taatgttttg gtttcagaga agctttaaaa agcaagttgt
    19921 tgaatgaaaa atcaaagatt taatctgatt tttcattttt atgctgtctc cacatgtcaa
    19981 aaagagtgca aatcggaaaa atcttgtaat tttgcaagtt gtcggaatat ttaaaaatcc
    20041 ggttttccac attaagcccg aaaaacttta aaagatggta ttgaattaca tttggatagg
    20101 tttttttctg attgcattcg ttattgcatg cattcaactg ttggttttcg gaaatgttca
    20161 ggttttttcc gacatgctca attcaacttt cgattcggcc aagtcaggtt ttgaaatttc
    20221 attgggactt acaggcgtat tgtcgctgtg gctgggtata atgaaagttg gcgaacgcgg
    20281 tggcgtaatt cgattgtttt ctaaggctgt ggcgcctttt tttgaaaaaa tttttcctga
    20341 tcttccgaaa aatcatcccg cttatggcag catgttcatg aatattgccg ccaacatgtt
    20401 gggactcgac aatgcggcta ctccattagg gctaaaagcc atgaaagaaa tgcaggaaac
    20461 caatcccgta aaagatactg cttccaatcc catgattatg tttctggtgc tgaatacatc
    20521 aggattgaca ttaataccgg taaccatcat ggtttacagg gctcaaatgg gagcaacaaa
    20581 cccttccgat atttttcttc ccatcctaat ttcaacattc atttccactg taagtggttt
    20641 atttatcgtt gctgcttacc aaaaaatcaa cattctggac aaaaccgttt taggaacttt
    20701 ggccggttta tttattattg taggcagtat tttgtatttt ttcagcaaag ctactcaaca
    20761 tcaaattgct ttgtattcaa atttcattgc cagttttgta ctgatttcca taatcatttc
    20821 ttttattttg gcagccatga ttcacaaggt aaatgtgtat gacgctttta tcgaaggtgc
    20881 aaaagacgga tttcaggtag caataggaat cataccttat ttaattgcca ttttggttgc
    20941 cattggtatg tttcgtgctg cgggagctat ggatatgctg attcgtggtt tcacgtggtt
    21001 tttcggactg attggatgga ataccgattt tgttggtgcc ttgccaaccg ctttgatgaa
    21061 accgctgagc ggaagtggag cacgcggtat gatggtcgat gccatgaaac tatatggggc
    21121 cgactcgttc gttgggcggt tgtcgtgtat cattcaaggc tcgaccgata caacttttta
    21181 cattttggct gtatattttg gctcagtagg aataaaaaac actcgatatg ccgttatttg
    21241 tggactttta gctgattttg caggtatagt agctgcaatt ttaatcggtt atctgttttt
    21301 tcattagttt ttctattagc aacttgatat ttgataagtg aacagatttt tttgaaaata
    21361 atttttccat gaacgagata attgaatata ttattaaatt cctgttgggc gacaacgtat
    21421 cggacgacat tgttcagaaa atcggatata ccgacaatga agaggaattt ggtcgttata
    21481 gactggttat ttattcttcc ggatttttca aaccggaaat gtacggctca atacattcca
    21541 ttccatcctt gccattaaaa atttgggacg atactccttt gctttatgga gaaccggaaa
    21601 tagaagaagt aggaaacgta cgtattattc atgccgatat aattgcaagc acctattttc
    21661 ttatttcgcg ttacgaagaa atggtacgtc gtggatgccg cgatgtacac ggacgatttt
    21721 acggaaaaga atcgctttcc tataaagccg gcttcattga ccgaccgttg gtggaagaat
    21781 acggaaaact tttgcgtcaa cagttgcgtg aattgggtat tgatattccg gaacccgaga
    21841 aaaaaatcaa aaaaattttt ctaactcacg atgtcgacca gctggctcat tacagacgct
    21901 ttaggggaat gatgggagcc gtagctcgag gtataaaatc gccaaaaaaa cgtgcagaag
    21961 cacgaagagc cataaaaaca tttttcaagg gcatccgatt cgacccttgg tacactttcc
    22021 cttggctttt caaaatagat caatcactga cagaagctat cggccaagaa cgatgcaaaa
    22081 cggttgtatt tatcaaagcg ggaggcggaa ataaaaagga agacaaacca aactccaacc
    22141 tggcacaccc cgattacaaa aattttattc gctactgtaa aagaaaaaat attgttattg
    22201 gccttcatgc aagttacgag gcagggcgat acccaaatct gattgcagaa gaaaaaaaga
    22261 aactcgaaca tgcagcaaaa accaaaatta cttacaaccg acatcatttt ctgtgttcgc
    22321 gcgaaccgga ggatatggaa gcacttatag aggcaggctt aaaggatgac tttacactat
    22381 catatgccga tatggcggga ttcaggctgg gaacttgtcg gcctgttcaa tggattaatc
    22441 cggccacccg acaactcaga ccgctgatcc tccatccttt taccattatg gacagcacat
    22501 tgagtgataa gcgctacatg aacatgaatg tatttgacgc ttacgaatat tgcgttcaat
    22561 tgattaacaa cgtagaaaat tacggcggtg aatttgtcat tctttggcac aatactatgg
    22621 tagaaaaaaa atcgccctcc tatcatagaa aattatacaa aaacattatc gattttctga
    22681 aaacaaagta agatgcaaaa aattgccatt gtatgcgtaa caaacgattt ggttaccgat
    22741 caacgcgtac acaaaacctg cctgactttg cagaaatgcg gtttttttac catcgaagtt
    22801 ggcagaatgt tacctgaaag tttaccattg caacgtcctt atgttacaaa acgacttaaa
    22861 ctttgctttc gcaaagggcc gcaattttac gctgaatata atatcaggct gtttttttat
    22921 ttactttttg ccaaagttga cttgatttat gcgaatgatt tggatacact tccggcagct
    22981 ttcttggcag ccaaaattcg cagaaaaaaa ataatttacg atactcacga atattttacg
    23041 gaaacacccg aattggtgaa tcggccaaaa gttcaagctg tatgggaaaa aattgaacat
    23101 ttcatatttc cgaaactttc ggatataatc acagtaaacg attcgattgc agaactttac
    23161 aaaaaaaaat acaaaaaaaa tctgcaagtg gttcgcaata tccctcctac ttaccttcct
    23221 cctcgattga aaaccagaaa agaattgggt ttacctgaag acaaacatat tttgatactt
    23281 cagggaaccg gtatcaataa agatcgagga gcagaagaaa tagttacagc tatgcaatat
    23341 ttacaaaact ttatattgct tattattgga agtggagata tgctgccgac tctgcaaaaa
    23401 atgacaatgg aactgcaatt ggaagataaa gttattttta agcccaaaat gccgtttgaa
    23461 gaattacgtc aatatacctg caacagcgat ttgggactgg ctgtcgataa agataccaac
    23521 ctgaattacc gattttcttt accaaataaa cttttcgact atattcatgc cggcattccc
    23581 gtgctcgctt ccaacttacc ggaaataaaa aaaattatta accgttacga tatcggctat
    23641 tttttggaaa atcacgatcc aaaaaacatt gcttccacca ttgaacgtat ttttgaaaat
    23701 gatgaacgct acaaaatact gaaaaaaaat acggaaaaag ctcgaaaaga attgtgctgg
    23761 gaaaacgagg aaaataattt gcttcaaatt ctacagcaat attcataagt accatacaaa
    23821 aatttaaaaa atatcgatat ttgattgaaa aacagtaatt aaaaccactg ttgcaatgaa
    23881 gttaagacca tgtttgaaat ttttattttt gaagcaaaca agtaaaacaa ctgattaaaa
    23941 attttataaa tccaatgttc gccaacaaat tcacggtctg ccactaagta ttgaatggtt
    24001 tcttttccaa acaatcgaat ataacgattc acgatttgaa ttctctcttt tgtatgggaa
    24061 atccctcgtt taggcattaa ttaaataaaa tagcttagta catgacaaaa attgtacaaa
    24121 tatttatact atttattttc agatatttaa taacgtgaat ttgccccgaa ctccacttac
    24181 ttgtttgtaa ttttcatcag taaagacgtt gcatgcaacg tctttacaag aaaaattgac
    24241 gttcgttggc taaaatcttc taaactcgct ccttcgtcgc tcaaacaagg acgattttta
    24301 aacgtttttc tcacttgttt tttcggctca ccggaaaaga tcaacccgaa gagcttggct
    24361 tatatgtatt gttttacaga tatataaaac aaatgtctaa atatttgtca tgtactaaaa
    24421 atgaattatt taaaaaaaat tcagttggcc aaaagaaata tcaaaaggaa agctaatcgt
    24481 taagaaattt gcattatctt tgcgcaaatt aaaaaatact caaatgtgaa aggcaaaaac
    24541 actgtcttat tgatattcac cggcggaacc atcagcatgt ctgaagaccc aacaacagga
    24601 gcattgcgtc caatagattt ttatcgcctg caggaatacc ttcccgaact caaacaaacc
    24661 ggaatcgaaa tcaaaagtat ccccttttca cctttaatgg attcgtcaga tatacaaccc
    24721 gaaacatgga aagaaattgc attaatcatt gaggagaact ttgataattt tgatggcttt
    24781 gtggtgcttc atggcaccga tacaatggct tattctgctt ctgcactgag ttttatgcta
    24841 caaaatcttt caaaaccggt gattttcaca ggtgctcaat taccaattgg catgatgcgt
    24901 tccgatgcca aagaaaattt gctgactgcc attgaaatag caactgcaaa agaaaatggt
    24961 aaagccattg tgccagaagt ctgcatattt tttgaagata cgcttttcag aggaaatcga
    25021 accacgaaga aaaacgccga acattttaac gcctttaatt catacaatta tccccctttg
    25081 gcaaaagttg gcgttcatat caaatatttt aattcctata ttcactatcc cgaatctttt
    25141 tccaaactcg aagtcaaaac caacattaat ccgaacattg ccattcttaa gctttttccc
    25201 ggaattacac agcaaaatgt tcatgctaca ttaaatattc ccgaattaaa agccgtcatt
    25261 cttgaaagtt atggcgctgg aaacgcacca cgccttatct ggttatatga agaattgaaa
    25321 gctgccgtag aaaaggggat tattattgta aacaaaagtc agtgcagcac gggaactgta
    25381 gaaatgggca aatacgaaac aagcttaaat ttgctgaatg caggtgtcat aagtggttac
    25441 gattgcacaa ctgaagctct ggtagccaaa ctcatgcatt tactgggaga atatcattca
    25501 acagaaataa tcaagcacaa gttaagtgtc agtctttgcg gtgaaatgac agtaagctaa
    25561 atgtgaaaat tttataaagc actccaaaaa tatacttcaa tcaaaattta ttttccaaaa
    25621 atcgatttaa agaaaccttt tttggtttgt ctgaaagcag gttgttctcc attcaaagca
    25681 aaaggattcg ctacactatt ggcagcaatc atttgataaa tcacgcccca atctggcaaa
    25741 cctcccaaat ttctgtcatc aatgtataga tcggcacgta tcttgcgcga ctcggcataa
    25801 tcgtctcctt cttccggata atttttatta atggcataaa attccaatcc tctcatttta
    25861 caaaattcta tggcttcctc cagttctctg ccttcgcgaa cagtccaaag aataatttga
    25921 tgctgaaatt catcttgaag acgtttcagg gtttcgatgg caaaaggtat ttctttccct
    25981 attttgggat attcatgcgt tactatggtt ccatcaaaat caacggcaat aatcataata
    26041 aaatcttttc ggttagaact ttagttgtct gggaaaacta tttttcggca ggaatatttt
    26101 cttctttgtc gagctgaagt tcctcaatca ttttcatttc agatggttta caaaaaatca
    26161 gaccggctgc agccatagct gcacaaaaaa taatggattg agaacgaagc agatatgcaa
    26221 aaataactcc caccacaaaa cctaatccta aaacaataat tcgatagata gaagcttttt
    26281 tatatttttc tatccgaata cttttatcgg gaataagcaa ccactttttt acttggaaat
    26341 gaaaaagtcc aagacttaag ggaatcgata aaataataaa caaaatgaat gctgaggaaa
    26401 gattgattgc tatagaactt tgagcctcta taccgacata atttttaaaa ataaatacat
    26461 atcccaccac tgctgccaca acggcagcca gataaacaga ataatataca agcaaaagtg
    26521 ttttaattat ttttcctatc atacgaactg aacaaaaaaa tttatttatt aaacgtctga
    26581 ctgaattcaa tacggttttg aatggagcgt ccaagcgtaa tttcatcggc atactccaac
    26641 tcgtctccta tagatactcc acgagccagc gtagtgattt ttacatgaaa gggttcaagc
    26701 ttacgataaa gataaaagtt ggtcgtatcg ccttccattg tggtactcaa tgccaaaatt
    26761 acttctttta tgccatcatt ttcaactctt tgaacaagac tgtcgatttc caaatcttta
    26821 ggcccaacgc catccattgg tgaaataatt ccgccaagca catgatataa accccgaaat
    26881 tgctgcgtat tttctatcgt aataacatct ttgatatttt ctaccacaca aactacggat
    26941 ttgtctcttg aaagattact gcaaatatcg caaatatcgc tatcggaaat attatgacaa
    27001 attttacaat acacaatttc cttccgtagc cgaataatgg aatttccaaa attctccact
    27061 tcttcttccg attccttaag cagatgaagc accaatcgaa gagcagtttt tctaccaata
    27121 ccgggtagtt tggcaaactg atgaatagca tcttcaagga gcgaagagga aaattgttgt
    27181 tgactcataa acagataata caaactttct gcaaatttag taattttttc ccaacaaact
    27241 aagagtaaga ggggaagtta ctttaaagtt ttttgtctaa atttgccact cgaaaattca
    27301 aatgataaca agcatgtcag gatttgcaat tttattgatt attgccgcat atttttcggt
    27361 tctactgttt atttctttta ttgtaagcag aagaagcaag gataacgatg ctttttttct
    27421 gggaaatcgt aaatcgccct ggtgggttgt tgccatcggc atggtgggtt cttctatttc
    27481 gggtgtaagt tttgtttctg taccaggcat ggtaaacagc attgatttta cttacatgca
    27541 gacagtattt ggttttttct tcggctatct gattattgcc aatgtattac ttcctgtata
    27601 ttataaactc aatcttacca ccatttatac ctatcttgaa aatcggtttg gcaagcactc
    27661 ttacaaaacc ggagcatctt tttttctact atcgaaaact attggtgctg ctgctcgact
    27721 atatattgtt gcactgattt tacagaatct tatttttgaa gcttggggag tcccttttga
    27781 acttactgtt tgtggaatca tcatactcat ttggctttac acccatcaaa gcggaattaa
    27841 aaccattgta tggacagata cgctacaaac tattatttta atcacggcac tgatactcat
    27901 tattgttgag gtttcccaac aattgaatat gggtgtgaag gatatttaca gagaacttgt
    27961 taattatccc aaaacaagaa tttttgtttt tgatgattgg aatgacaagc aaaatttctt
    28021 taaacaattt tttagcggta ttttcattgc aatagttatg acaggacttg atcaggatat
    28081 gatgcaaaaa aacttatcgt gtcacaattt gcaggaggca aagaaaaaca tgtactggta
    28141 cggattttct ttcgtaccgg ttaattttct gtttcttgtt ttgggcgcat tgctgcttgt
    28201 tttttccgcc gaaaagggta ttgccttgcc tccattgggc gacgaaattc tgcctatgtt
    28261 ggctgcaaat tatcttggtc aaacagtaat gatttttttt attacaggta ttattgccgc
    28321 tgccttttcc agtgccgatt ctgcacttac agccctaacc acctcttttt cggtagatat
    28381 tttgggcatt cagcatcaag aaagccgaaa agctaatcac acacgccgca tcattcatgt
    28441 ttgtatttcc atattttttg caataattat tgtgattttc aaagctatca acaataaaag
    28501 cgtaattgat gccatttata ctgtagctac ctatacttat ggacctttgt tgggaatgta
    28561 tactttcgga cttttcacca agatgcaggt aaaagacaaa ttcatgccat atattgcagt
    28621 cttatctcct attctatgtg gattgatgga ttatttattt acgcgttact tacattattc
    28681 tttcggatac gaaatactca taataaatgg attacttact tttacaggta tgtggatctt
    28741 cagaaagaaa aacataattt ttaacaccat aaagtcataa atagaaatgg aaatacatgc
    28801 gacagaattt ataaaaagca gtactgacta caagggatgt cctgaaggga actttcctga
    28861 atttgcattt atagggcgtt ccaacgtggg aaaatcatct ctcatcaata tgctttgtca
    28921 gcgaaaaaat ctggccctta cttcttcgaa acccggtaaa acactgttga tcaatcattt
    28981 tttaattaac aaaaactggt atttggtaga tttgccaggt tatggatttg ctcaaaccgg
    29041 aaaagaaaac cgaaaaaaac taatagataa tatcaataat tatattcttt ttcgtgaaca
    29101 acttacatgt ttgtttgtgt tgattgatgt gcgtcatgaa ccacaaaaaa tagatcttga
    29161 atttatggaa tggttgggat caaatggaat tcctttttgc atcgttttta ctaaaaccga
    29221 taaactttca gcatcgaaaa tcgacgaaaa tattgaaaat tacaagcaaa aaatgttgga
    29281 aaattgggaa cagttgcctc cattttttaa aacctcctca cataaaaaaa tcggtagaga
    29341 agaaatatta aattttatcg ataaaataat cgaaagcctg aaagtgcaat aaaatctctg
    29401 cacttaacaa agaaaagagg ttaaaaaaac atattacctt ttcaaagttc tttctatgta
    29461 tcttttatct tccttttctt ttaatgtttg tcttttatca taaagcttct tgccttttac
    29521 caaagcaatt tccaattttg ccaaacctct ttcattgata aaaagtttta agggaacgat
    29581 agtattacca ctttctttta ctgcacgttg aagtcgtttc aattcctgcg ctttcaacaa
    29641 taatttacgg tctcttcgag tttcatggtt attgtagcta ccaaaaaaat agggagctat
    29701 gtgcatattt ttaacccata attcatcatt aataaatgtg caatatgtat cggatagact
    29761 tgccttgcca cttctaatag atttaatttc tgttccaaac aattgaatac cgctaatata
    29821 acgatctaaa atttcgtatt caaaagtggc acgtttgttc ttaatcacta tattggatgt
    29881 tttatgcatc gcattaaaat ttttgacaaa taattgttgt ttacaacttc taaaacaaac
    29941 aagaaaaaat tgggaattca aaattacata taattctttt aatcaccatc agatgcacaa
    30001 aacaatcaga ttattctgtt ttaaaacata ttttttaaac agataaatat tctgatttaa
    30061 cctaaatttg tgcattcaga aactataaaa aacttactta atatgactca acaggaatta
    30121 aaatcagctt ttgaaaaaat ttacggtcag cctgcggatg cagtttattt tgctccggga
    30181 agggtaaatt taattggtga acataccgat tataatggcg gttatgtttt cccttgtgca
    30241 cttagttttg gaacttatct gctcattcgt aaaaatggcg gaaatacagt tcgtttccat
    30301 tctttgaatc agccgaatgc cgtagaagtt cctttggaaa aattaactac tcgactcgaa
    30361 aattcgtggg caaattatct gattggtgtt tttgcgcaat ttatcaaaaa aggtgtcaaa
    30421 atcgatgaag gttttgatat attgatttgg ggtgatgtgc caaccggtgc cggattgtct
    30481 tcttctgctt cattggaagt cgttacggct tacgcattga atgatcaact gggaacggga
    30541 tatgaccgca cagaattggc tctgatgtcg caaaaggcag aacatgaatt tgcccttgta
    30601 aattgtggca ttatggatca gtttgctgct gcgcaaggga aaaaagatca tgccattttc
    30661 ctgaattgcg acacactgga atttgatttg gttcccgtaa aattaaatgg tattaaagtt
    30721 ctgatttcca atacacacag cccccataag ctggattccg gagcttataa ccaacgcgtt
    30781 gccgaatgta aaaaagcagt tgagcaattg aataaggttc gttcaataaa atatctggcc
    30841 gaactgacgg aagaagaatt taaaaaaata gaatcggcga ttaccgacga gacggcaaaa
    30901 aaacgtgctc gccacgttgt gagcgaagtt caacgcacca aagatgcggt aaaagctttg
    30961 agagccggcg atattctcca gtttggaaaa ttaatgaatg cttctcatgt ttctcttcgc
    31021 gacgattacg aagttaccgg ccctgaactc gatacgatgg tagaagaagc ttggaaaata
    31081 gatggcgtga taggttcacg aatgaccggc ggaggctttg gaggatgtac cgtttctctg
    31141 gtaaaagatg aagcgatcga cacttttatt aaaaatgtgg gagccgctta tgaagcaaaa
    31201 atcggaatca aacccgaatt ttatattgcc gaaataggtg atggcgcttg tcgattgagc
    31261 tgatttattt tcaatcaaaa caaaaaccta atatttattg aaatgattta tgatatttca
    31321 ataactgcaa tttaaacagg agttagacat gaaaagcgag gaatcttcac aaataacagt
    31381 tcacggtaaa acagtaaaac tatttacttt aactaataaa aacgggatgt catgcagcat
    31441 caccaacttt ggagcaaaag tggtcaagct ttttgctgcc gataaagaag gaaaatttga
    31501 agatgtggtg ttgggttttg atacattaaa cgaatgtatt gaacgcgaac cttacttcgg
    31561 agctgtttgt ggtcgctttg ccaaccgaat taaagatggc aaattcgttc tcgatggcgt
    31621 tgaatatcat ttgcccatca acaacggaac aaattctctt catggcggca ttcatggctt
    31681 caatgaaaaa atctgggaag ttaaatcggt tgacaatcaa gaaattatta tggaatatct
    31741 gtcggtagat ggcgaagaag gttatccagg caatctcacg gtaaaggtaa cttattttct
    31801 ttcggacaac aacgagttaa aaattcatta cgaagccgta accgataaag caacggtcat
    31861 caatctgacc aatcattcct atttcaatct caaaggagcc ggcaacggaa ctattcgcaa
    31921 ccacatatta cagttgaatg ccgattttta caccgtactg gacgattctt ttgcgccgac
    31981 aggagaaatt cgtcccgtag aaaactcggc tttcgatttt cgtcaaccca ctgtaattga
    32041 acagcggatt gatgatgagg cttttgctcc gggaagagga ttagacaaca attgggctat
    32101 tcggaaacat catacaggag aaatggttgt agccggattt ctgtacgaac ctgaatcggg
    32161 cagaaaaatg gaagtcatga caacacaacc gggagtgcag gtttattcgg gcaactggat
    32221 agacaaacaa acggggaaaa acggaaaagt ctatgaaagt caacatgcca tttgtctcga
    32281 aacgcaagga tttcccaatt cagccaatgt acccttcttc cccactcccg tattacgtcc
    32341 cggacaaaaa tacgatgagt ggtgtattta cagattttcg gcggaataag gaagggaaaa
    32401 taaattatat ttttcctgta agtcgatgct tttcaaatca tagggaaaca ttttaacgac
    32461 agaaattaga gagaattttt tctaatttct gtcgtttatg gctcacatgg aaaaatatga
    32521 gtttgtagaa aaagagaagt atctttgtga aaaaatactt tttatatgtc cacttacgaa
    32581 cagcttgagg cacttgcccg ttacattctt ccgaaagaga tattagacga ttttgatatt
    32641 gttggaattg aacccaaatt actcattagc caatttaaag ggaaagtcaa acttacaaga
    32701 actgttccat aggccggaaa atcatgttcg ccttttttat ttaaagcgat ttacagaagc
    32761 agtttgttgt tgattttttt caaaatatgc accaatggca aaagttttat atctaagtgt
    32821 gtagataaag ttttctgcgt ttttctctgc aaaatgaaca ttctaaacag cgacataaga
    32881 ttataggcta tcatcacaaa attaaagacg aaagtgcttt cctttaattt ttgacgaaca
    32941 ataacgattc tacgtggttt ttcaaacatt tgttacaaaa aaatcattcc accacatggt
    33001 gttatttctt tgtttgtaag gatataccaa agttcacaca cgtttttttc tattgctact
    33061 cataagagtt tcctataaaa ttaaaacttt ttttcctatt gcgaactcta agtttatcaa
    33121 aacgacatga gtaagtacat ggaaagtttt gaagttattc atctttcaat aaatgtttct
    33181 aaaaaattag gctgtcagcc attgcaagtc ttttcgcagt tgttccaagt tttttctaag
    33241 tcgtttaatt acaaagttat taaaaaggaa agtaagactg ctcttgataa tttggttggg
    33301 taaatgcttt actttggaaa tttctaccac acgattcaat tgagcggcag caaaatgtac
    33361 agatattttt cccattgaat tgcgtttaat ccgggtaaag acgtagcatg ctacgtctca
    33421 tatacaacat ccaaaagtgt tattgcaatg atagaatgga acgcatgatc tgaattgggg
    33481 gaatgcgcgt ttgtttccct attgaattgc gttgcaacat gtgttgttag aatgatggat
    33541 tggaacagga aaatgtactg tgttcgatgt atgacaaaac cattattttt tctctattga
    33601 ggtttcaaat tttacagcaa acataaattg ttaatttttt ataaaaaata ttttgatttt
    33661 aaaccttgac ttgattcata agtcaaatta taaaatttaa agaaaataag aattttaaaa
    33721 acaaaagttt atgaaaaaga ttttaatttt gatggttagt gctttatttt tgataagtgt
    33781 ggcaggtcat gctcaaaatc aaaaaaatgt ttctgacaaa gaagggaaaa aaagtgaaat
    33841 gagacaaaat gcatcacaaa gaatgtcagc tgaaagtcgt gcagaatatt gggctaaaga
    33901 gctgaatctt accaatgaag aaaaagcaaa aattgcagaa ttgtttaaaa aacaggatga
    33961 aacaagaatg caacatcagg cagaaatgca aaaatcaaga gatgaacatc gggcattcat
    34021 ggaaagtcaa cgaaaagcaa atgatgccga aatggaaaaa atcattggaa aagaaaaatt
    34081 tcagaaatac gattctattc gtatggcaag aatgcagaca atgagaaaca gaatgaggaa
    34141 tcgataattt ttttctaaaa gtttttaata agaatcttga attttggctc aaaatttgtt
    34201 agacattgaa aaaaaagatt gtgttgaagt atataggtaa tttttaaggt aacgaggctg
    34261 tctaaagaat aggatagcct ctttttttta attcatcaga atataattta ttttttaatg
    34321 gtctgatgga acccacatgt ctgaatacgt gtttgtgtat ctaatacatg tgggtttcat
    34381 aatatacaac acaatttttt ggggaaattt tttcaaccaa tagaaaattt attcctttta
    34441 aagcagactg aaaatttttc aaaaagttgt aactttgcag gctaaaaaca agtaataaag
    34501 aatttatcta tcgtattcaa aatgagtttt caagaagaaa ttcgacgcag aagaacattt
    34561 gccatcatca gtcatcccga cgccggaaaa accaccttaa cagaaaaact tttgcttttt
    34621 ggaggagcta tccatgttgc cggtgccgtg aaatcgaata aaatcagaaa aacggcaact
    34681 tcagactgga tggaaataga aaaacagcgt ggaatttctg tagctacttc cgtaatggga
    34741 tttgattatc aggattacaa aattaatatt ttggatacac cgggtcatca ggattttgct
    34801 gaagatactt acagaacgct tacggccgtc gacagcgtga ttatagtgat tgacaacgcc
    34861 aaaggggtag aggaacaaac gcgcaaatta atgaatgtgt gccggatgcg caaaacgccg
    34921 gtcatgattt ttgtcaataa aatggatcgt gaaggcaaag acccattcga tttgctcgat
    34981 gagctggaat cggaactcga tattcacgtt cgtcctttga gttggcctat caatcagggt
    35041 ttttcattta agggagttta taatatctac gaggaaaatc ttcagcttta cacccccaat
    35101 aagcaaacta tcagcgaatc cgttgagatt tcggatattg acagtgagga gctggaaaag
    35161 ttcattggag agaaagatgc acgccaactg aggcaggatt tagaactcat actgggaata
    35221 tatccggctt tcgacaggga aaaatatctg tcgggagagt tggctccggt tttttttggc
    35281 agcgctttga acaatttcgg agtgaaggaa ttgttgaatt gcttcgtgga aatagctcct
    35341 tctccgcaac ccgttcaggc tcacgagaga gttgtaaatc cttacgagga aactttttcg
    35401 ggttttgttt tcaagattca cgccaatatg gatcccaatc atcggagttg tattgctttt
    35461 gtgaaaattt gttcgggaaa atttgaacga aatgtcaatt acaagcatat tcgacaggga
    35521 aaaatgttgc gtttttcaag tccaactgct tttatggcac aaaaaaagga aattgtggac
    35581 gaagcttact ccggcgacat tgtgggattg cccgataatg gaaattttaa aattggcgat
    35641 acgctgaccg gtggagaaga tctccatttt aaaggattac cgagtttttc gcccgaaatg
    35701 ttcaattatc tcgaaaatgc cgatccgatg aaaaccaaac aattggaaaa agggattcgg
    35761 caactgatgg acgaaggcgt agcacaactt tttaccagcg aatttaatcg aagaaaaata
    35821 gtgggaacag tcgggcagtt gcagtttgaa gtaattcaat accgattgtt gcacgaatac
    35881 aacgcgcaat gccgttggga gccgatttcc ttatataagg cgtgttggat agaaagcgat
    35941 gatgctgaac agctcgaagc cttcaagaaa cgaaaagcgc aatttatggt gaaagacaaa
    36001 gaaggccgcg atgtatatct cgccgagtcg ccctatattt tgcaaatggc tcaacgcgat
    36061 tttgatcata tcaaattcca tttttcttcc gaattttaat ttctatttca actaagaaag
    36121 atggaatttg cgtagaaaaa ttaggcttct ccgcctccaa atcccatagg aaaaggggga
    36181 ataaaagaat tttcatcaat ttttattttt ccgttttgct gttcatattt tgcaatattc
    36241 tcctgcagtg catacaataa ccttttggca tgttcggggg tgagaatgat gcgtgatttg
    36301 acgggagctt tgggtaatcc gggcataatg cgaataaaat cgagcacaaa ttcggaagaa
    36361 gaatgtgaaa taatagccag attggaatag ataccttctg ccacttcagc agaaagttct
    36421 atattgattt gattttcatt gttttccatt gtcttatatt ttaattaatt tatttttcac
    36481 ctttgttgaa accgcgttgc aaaagtagca aaatatttat gtgtgtgaag aaactatttt
    36541 agcattctat gtactaaaga gagaaaaaat tgtataatcc agcaaatgaa ttatttattg
    36601 tccagttgtc cgaaaacgcg tttcagtaat gttgtagtgc gggctgcagg gtccgtgcga
    36661 atggaatttt cagtttctgc cattttagta aacaatgcac tcaatgcctt gtcggtaaca
    36721 tattgatcca attgggtgtt aacaggtttc agagaagcaa gtttacccaa agtagaattg
    36781 gctgcgctgt tgtaggcatt tatcagcaaa ttccatgttt ctgtagttga aacatcacct
    36841 accaatggtt tctttagcga tgcctgaact ttaggtagaa aaagattttt caattccgtt
    36901 gaagtagttt tttgcaaata ttgcgttgct gcattgcttc cgcccagcaa aatacccatt
    36961 gcatctgaaa tagtcatgtc ctgaatcgct tttttaaaca caggacctgc ttcttttact
    37021 gcatcttctg ccgaacgatt gatggacaga atggctttgt cgaccaaagc tttgcctccg
    37081 ggtatcaaac tgatattttc tacaatcgtt tgagcttctt caggcaacag taatttaaat
    37141 gcctgattac cataaaagcc attttctata cttaaatcgg ataccgctct ttccgttccc
    37201 aataacaaag cattttttat acccgcaatg ttttctttgt cggtggttgg aatagataat
    37261 gttcctgaat tggaaacacc ctgcataaga acattacatg aagtaaggga aatacaaatg
    37321 aagctaaata aaataaattt tgttgttctc atcgctttat tgttgcaaaa tatttaattt
    37381 tcatttttaa gaaggtaaat atacgctatt ttttgatatt ttaagtacgt atataaacaa
    37441 aattaaaaga acattttata actttgcaga aaatgtaaaa agaggaattt ctaacaaatt
    37501 atcaatctta attataaaga aaatgagtag aatagaaaaa atcgttgctc gcgaagtatt
    37561 ggattcaaga ggaaatccaa ctgtagaagt agatgtaatg ctcgaatcgg gcgttatggg
    37621 aagagctgct gttccgtccg gagcttctac gggagaaaat gaagccattg agttgcgtga
    37681 tggcgataaa aaacgctatc tgggaaaagg cgtactgaaa gccgtttcga acgtaaataa
    37741 cattattgca ccttcattaa ttggtatttc tgcattggat cagcgtacta ttgacaagaa
    37801 aatgattgag ctcgatggta cgaaaacaaa gtcgaatttg ggtgcaaatg ccattttggg
    37861 agtttctttg gctactgcaa aagctgctgc agcttatctc gatctgccgc tttaccgata
    37921 tattggtggt gtaaatgctt ttgttttgcc gattccaatg atgaacatta tcaatggagg
    37981 ttcgcattct gatgctccca tcgcgttcca ggaatttatg attcgcccgg ttggtgctcc
    38041 ttctttccac gaaggtttac gaatgggagc cgaagtgttt cattcactga aaaaagtgtt
    38101 gcacgaccgt aatctgagca ctgctgttgg cgatgaaggc ggatttgccc caactttgaa
    38161 aggtacggaa gatgcgctgg aatctattat aacagctatt gaaaaagccg gttacaaacc
    38221 cggacaggat gtaatgattg gattggattg tgcttcgtcc gaattttacg aaaacggtat
    38281 ttataattac accaaatttg aaggagcaaa tggcgccaaa cgtacttctg aagaacaggt
    38341 taaatacctc tcagaattgg tcagcaaata tccgatcgat tctattgaag acggtatgag
    38401 tgaaaacgac tgggaaggct ggaaactatt gaccgaaaaa cttggaaaca aggttcagct
    38461 ggttggggac gatttgtttg ttaccaatgt agaatttttg aaaaaaggta tcgaaatggg
    38521 ttgtgccaat tccattctta tcaaggtaaa tcagattgga acattgtccg aaaccttgga
    38581 tgctatagaa atggctcatc gtgccggata tacaacagta acttctcatc gttcgggtga
    38641 aacggaagat gctaccattg ccgatattgc tgtagcaacc aattccggtc agatcaaaac
    38701 cggttctttg agccgttcag accgtatggc caaatataac caactgcttc gtatcgaaga
    38761 agaattggga gacttggctg tttatggcta caaaaaagtt acaaaaaaat gacagtataa
    38821 attgtcagtt ttttgttttt aaatcaagaa ccacctgtca aaaaggtggt tttttgtttt
    38881 agtaacaaaa tttttctaaa aaatctgagg tctttctgtt aattcccgtt aagctgcaaa
    38941 actctgtgtt aaatttcgac aaatagctat atcactttgt gttatggaat gtattttgta
    39001 tactttagca gtgaaaaatt tgtttaagaa tattttttca gaaaaatcaa tttaaatcta
    39061 aaaaacgaaa agtatggaag catcgaaaag aaaaaaagcg ttaggcctat tcttgttggt
    39121 ggtagtagtg agtgtggcaa caaatgtgac aaccaattac tttaccaata aacatgagaa
    39181 agataatttt aaacgagaat tgcagtatcc gacagcttat gcaaaatttt ccaatgtgaa
    39241 gagtggtgtg gaaaccgatt ttacattggc agctgaactt acgattcatt cggtagtaca
    39301 cgtaaaaaca caatcaaaag caaaaatggc ttttagggaa gatccctttt ttgaattttt
    39361 ctttggccgt cctcgctatt atcaagaacc gcctatggta g
//
LOCUS       DAOSCH010000010        29675 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1153_length_29675_cov_6.054288, whole genome shotgun sequence.
ACCESSION   DAOSCH010000010 DAOSCH010000000
VERSION     DAOSCH010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 29675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29675
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1153_length_29675_cov_6.054288"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            918..1409
                     /locus_tag="PLZ54_01845"
     CDS             918..1409
                     /locus_tag="PLZ54_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOJ65715.1"
                     /translation="MKRIGILSDTHGYLDPRIYQYFSECDEIWHAGDWGSLEVVNQLN
                     ELKPVRGVWGNIDDVNIRSLFPQHFHFQCEDVAVWLTHIGGYPGKYDRSVRPAILQQP
                     PDLFVCGHSHILKVQYDEKLNLLLINPGAAGKYGFHTVQTIVRLEIDGKKIQNLEVIE
                     LEK"
     gene            1470..2057
                     /locus_tag="PLZ54_01850"
     CDS             1470..2057
                     /locus_tag="PLZ54_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOJ65716.1"
                     /translation="MFQVNDTLISENLLEEFFVCNLVSCKGICCVEGDAGAPLEKDEL
                     KIIKDILPEIQEFLPENSKKIIREKGFYYIDQDGDCVTSLVNERECVFAFFEEDGTCK
                     CSLEKAYYQGKTDFCKPISCHLYPVRLQKINDYTAVNLHHWKVCECAFVLGKKLKIPA
                     YRFLKVPLIRRFGEKWYKEVELIADLLKKTRERND"
     gene            2331..3575
                     /locus_tag="PLZ54_01855"
     CDS             2331..3575
                     /locus_tag="PLZ54_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOJ65717.1"
                     /translation="MNSKKVEIITIGDEILIGQIVDTNSAWMAQELNKAGFEVFQITS
                     VHDEASHITNALDEALKRTDIVLITGGIGPTNDDITKKTLCEYFHTKLVFDPSVIENI
                     KQVFANRIDFVFNDLTYSQAMVPENCTVIQNKVGTAPITWFEQDEKVVVSMPGVPYEM
                     MEAMRNEIIPRLQKKFQTQKILHKTIQVFGYSESALAIKLADWEKELPDFIHLAYLPN
                     YGIVKLRLTGTSDDLLSLEFAVNQQIESLKQLLGKAIIAFEDLPIEQIVGNLLTAQGK
                     TTATAESCTGGNIAHRFTSHAGSSEYFKGGIVAYANEVKTEVLQVSSEDLNLYGAVSR
                     QVVEQMARGALQLLHTDTAIATSGIAGPSGGTAEKPVGTVWIAACSKDKMTSRLFHFG
                     NTREQIIQRSTQAALVMLKEIT"
     gene            3898..5091
                     /locus_tag="PLZ54_01860"
     CDS             3898..5091
                     /locus_tag="PLZ54_01860"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOJ65718.1"
                     /translation="MFKVSERLAALSPSETLAMSQKSNELKAQGFDVINLSVGEPDFN
                     TPDHIKAAAKQAVDNNYSFYSPVPGFLELRKAICSKLKNENNLEFTPDQIVCSNGAKQ
                     AICNVLLTILNKDDEVIIPAPYWVSYPEMVKLGEGKSVFIYSDIENDFKISPEQLEKA
                     ITEKTKALILCSPSNPTGSVYTKEELQGLTQVLAKHPQILILSDEIYEHINYVGKHES
                     IAQFEEIRDRVVVINGVSKGYAMTGWRIGWIAAPKWIASACGTLQGQYTSGACSVAQK
                     AAEAAYTGDQSCVETMRAAFERRRNLVVELVRQIPGLKVSVPQGAFYVFPDCSAYLGK
                     SFNGKVIENSADLAMFLLETAHVASVGGVAFGAPQCIRFSYATSEDNLKKAFERIQQA
                     LNKLQ"
     gene            5325..5744
                     /gene="ybeY"
                     /locus_tag="PLZ54_01865"
     CDS             5325..5744
                     /gene="ybeY"
                     /locus_tag="PLZ54_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOJ65719.1"
                     /translation="MIQYFTENIPMPALKKQKINRWIKQTAHNYEKKVGEIAYIFCNN
                     ERILEINRQYLQHDYYTDIITFDDSTDSTISGDIFISLETVKSNAEDFSVDFEEELHR
                     VIIHGILHLCGQDDITPELKAEMTRKENKALEELKKM"
     gene            complement(6143..6889)
                     /locus_tag="PLZ54_01870"
     CDS             complement(6143..6889)
                     /locus_tag="PLZ54_01870"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOJ65720.1"
                     /translation="MSESFLSTVVALFLGSVGSVLLAGLILLLKERQLHRVTHVLTYL
                     AGGTLLGSALLGMIPEASDMMEIKKVLYVVLAGIIFFFVLEKIILWRVCSDASCERNL
                     QTAAPMVLFGDAFHNAIDGVIIASAFLTSNTFGWVVTLAVIFHEVPQELGDFGILLKS
                     GMKRSRALLYNALSGASALIFGIVAYFSLNFAKSLIPYALAFSAAGFLYIALADLIPE
                     MHRKTSFKESMVQIFFILFGIMLIYFFGRD"
     gene            complement(6934..7332)
                     /locus_tag="PLZ54_01875"
     CDS             complement(6934..7332)
                     /locus_tag="PLZ54_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HOJ65721.1"
                     /translation="MSRPRQKRIIKSPPLMMGYKPFGIPHKELDKIELHFDGFEAIRL
                     LDYEGMTQEEAAGHMNVSRPTLTRIYESARKTIAQALVEGKMIVIKGGNIEFEHQWFR
                     CKYCYQLVKEGETHVHQKYSDENELLPIES"
     gene            7551..8741
                     /locus_tag="PLZ54_01880"
     CDS             7551..8741
                     /locus_tag="PLZ54_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOJ65722.1"
                     /translation="MASTTGKSARLLSLDVLRGITIAGMILVNNPGNWGSVYPPLRHA
                     EWNGLTPTDLVFPFFMFIMGVSMYLSYKKFDFKFSNRTFLKLFRRSSLLFLIGLAIGW
                     LGLFSGGLRELQGTDIPLGERIWQAATNFENVRILGVLQRLALVSFFGTLIILLIPHK
                     HIPWLTGAILLIYWILIGVTGSYVASENNIIAIVDRAILGASHMYTETLPDGKVIPFD
                     PEGILSTLPCIAHVLIGFWAGKLISESKSKNEQIQNLAIFGTILFFAGFFLHYGFPVN
                     KKIWSSSFVLVTCGLASLLFSLLIWIIDVNNKKKWSVFFESFGVNPLFIYVVADILSI
                     LLSNVVFMYQGNWISIKNFVYFEWLSSVFGNYLGSLIFALCFVFINWIIAHILYKKKI
                     YIKL"
     gene            8861..9817
                     /gene="tal"
                     /locus_tag="PLZ54_01885"
     CDS             8861..9817
                     /gene="tal"
                     /locus_tag="PLZ54_01885"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOJ65723.1"
                     /translation="MNLLEQLKKFTTVVADTGDFASMMAYQPVDATTNPSLIFSASQD
                     PKYEYLMEDAIRYAQSLTSDKEEQLKKAMDKLAVNFGIKILEIVPGRVSTEVDARLSF
                     DVKGTIAKARELIALYESAGISRERILIKIASTWEGIRAAEILEKEGIHCNLTLLFSL
                     TQAVACAEAGVTLISPFVGRILDWYKKDRGVTDIPPEEDPGVKSVTAIYNYFKKFGYA
                     TQVMGASFRNMGEIIQLAGCDLLTISPALLKELEQTEGVLERKLDPEKAKTADIQPLK
                     INEISFRWMMNEDAMATEKLAEGIRNFTKDLLKLENKLAALL"
     gene            complement(9895..9965)
                     /locus_tag="PLZ54_01890"
     tRNA            complement(9895..9965)
                     /locus_tag="PLZ54_01890"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9931..9933),aa:Cys,seq:gca)
     gene            complement(10042..10500)
                     /locus_tag="PLZ54_01895"
     CDS             complement(10042..10500)
                     /locus_tag="PLZ54_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65724.1"
                     /translation="MKKFKYILMLMVWITVNSLSAEENANTNIQKMHEKKWAYIVEQA
                     KLTPDEASKVKPVFMNYENAVWELHEKNRETLNNFLKENKTQPNYELLNDEYVNLQIK
                     QAQLLKNYHLKLKKALSPEKLFHYYQAERTFKRKLLHNMPQGRKMQKNRP"
     gene            complement(10507..10887)
                     /locus_tag="PLZ54_01900"
     CDS             complement(10507..10887)
                     /locus_tag="PLZ54_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65725.1"
                     /translation="MKKEKNILKKIGNDLPFSVPENYFEQFANEIDRQIGYAKEMEVS
                     KSQKRSFAVISKTWLYMAAMFVGIFLLGNVFYLVFKNTERQKQENYEMYLLSQVDEPT
                     MMYYYMNDAYEAENGTETFQNTSN"
     gene            complement(10992..11564)
                     /locus_tag="PLZ54_01905"
     CDS             complement(10992..11564)
                     /locus_tag="PLZ54_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ65726.1"
                     /translation="MENDKNIKGEEKLKEQLQDPKRKAAAFSQLVRMYQERLYWHIRK
                     MVLSHDDANDILQNTFMKAWNGIDNFRGDSLVSTWFYRIATNETLTFLANKRMKNINS
                     LDEVEDVLLQQLQADQYFNGNEAQLKLQQAILTLPEKQRLVFNMRYFDDLSYEQMENI
                     LGTTVGALKASYHLAVKKIEKFLLDSETEI"
     gene            complement(11624..12580)
                     /locus_tag="PLZ54_01910"
     CDS             complement(11624..12580)
                     /locus_tag="PLZ54_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ65727.1"
                     /translation="MRVLVSTRLPEEGFSELMKKFEVVFPEKEIFSKEEVIQIIPEFD
                     AFIPTFQFKVDRDVIGAGAQGKLKIIANYGVGYDNIDVDFATKCGIVVTNTPDPVVEP
                     TAEMAIALMLTVARRVSECDRKLRTDSSIKWGVMENLGISLYGKTLGIVGLGRIGKAV
                     ARRAAALGMKIIYFNRNPLDKKTETKLKARYAPLGQLLCESDVISLHVPLTESTFHLI
                     NENTLRLMKPTAILINTSRGAVIDESALTEALTNHKIFGAGLDVFEHEPTIPENLKKL
                     DNVVLSPHNGTATIEDRNAMSRFASQNILRFFEGNKNISRVN"
     gene            12802..14046
                     /locus_tag="PLZ54_01915"
     CDS             12802..14046
                     /locus_tag="PLZ54_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOJ65728.1"
                     /translation="MAVVKPFRGIRPPREIVEKVASRPYDVLNSEEAREEAKGNPMSL
                     YHIIKPEIDFPEGTDEHDERVYAKAAENFKKFRENGWLVQDEEEHFYIYAQTMNGRTQ
                     YGIVVCAHIDDYLNGHIKKHELTRSDKEEDRMKHIRVTNANVEPVFLAYPSNEELNGI
                     VSEIVKSEPVYDFVAPDGFGHHFWKIDDKEIIHRITEIFQKIPSLYIADGHHRSAAAV
                     RVGDEKRRQNPHHTGKEEYNYFMAVCFPDNQLQIMDYNRVVKDLNQLDDEEFLQKLSQ
                     YFIVEKMGTEIYRPNKLHNFSLYLSGNWYSLTAREGTYNDNDPIEGLDVTISSKYILD
                     KILGISDLRTDKRIDFVGGIRGLGELKKLVDSGKMRVALALYPVSMQQLIAIADTGNI
                     MPPKTTWFEPKLRSGLVIHELS"
     gene            14128..16137
                     /locus_tag="PLZ54_01920"
     CDS             14128..16137
                     /locus_tag="PLZ54_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ65729.1"
                     /translation="MQFFIKKPSFILYRIISLCLLLLFLQSCGVSSRIKKADSRFELG
                     EYYAAGDLYKSSYGRIPNKDKSLRGYVAFRQGECYRLINNQRAEQAYLNAIRNNFADS
                     TVFLRYAQVLHRNGKYGEALRNYQLYLQKDSSCIIAKNGIRAIQMLEEWQKNATRYVV
                     KYDNQFNVRRASTFSPAFVNTAADELVFTSTRSTDKKATAKADPITGLPKNNLFLARK
                     NATGKWGKPELLEGEINTPETDDGVCSFAANGKTIYFTRAQQSNLVDMGTKIYVSSRA
                     GATWSPPQMLKIFSDSSISVAHPAIAPDGQTLYFVSDKKDGLGGKDIYRATLEGTDCK
                     YIENLGPEINTPGDEMFPVVRADGTLYFSSNGHPGFGGLDIFKAVPQKNGGWKVENMG
                     APVNSKADDFGMTFAGESESGYFSSNRNEMRGYDALWSFHLPELIYLVEGKIFDDQGQ
                     TISDATIRIVGSNGMNARIQTKKDGTYRIKLEKDVDYVMLATARGFLNQKNELSTQGL
                     TDSKTFNVNFKLSSISKPVKIENIFYEFGKWDLTPASEKSLQELVKLLKDNPNITIEL
                     SSHTDYIGDNAFNKNLSEKRAQSVVNYLIKAGIDKERLTAVGYGEEKPIVVDESLAQK
                     YPFLKVGETLTESYILTLTPEQQEIANQINRRTEFRVLKTTYNLY"
     gene            16218..17012
                     /locus_tag="PLZ54_01925"
     CDS             16218..17012
                     /locus_tag="PLZ54_01925"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOJ65730.1"
                     /translation="MKQYLDLLNRVLTEGVHKEDRTGVGTLSVFGHQMRFPLNEGFPC
                     LTTKKLHLKSIIYELLWFLRGDTNVKFLQENGVSIWNEWADEHGDLGPIYGYQWRSWP
                     DYEGGYIDQIAQAVEAIKNNPNSRRIIVSAWNVADLPKMKLPPCHAFFQFYVADGKLS
                     LQLYQRSADIFLGVPFNIASYALLLMMMAQVTGLKAGDFVHTLGDAHIYTNHLEQVKL
                     QLTRTPRPLPTMLINPEVKDIFSFQYSDFQLINYDPYPHIKGEVAV"
     gene            17120..17620
                     /locus_tag="PLZ54_01930"
     CDS             17120..17620
                     /locus_tag="PLZ54_01930"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOJ65731.1"
                     /translation="MPKISIIAAVADNYAIGNLNKLPWHLPADLKYFKKLTTGHAVIM
                     GKHTFENLPNGPLPNRKNIVLTSVLSDGICEGYFEANSIEEALELCEKEDEVFIIGGV
                     SIFKQTIDLADKMYITWIHGDFPADAYFPKINFEEWEEISREDHPADEKNPYPYTFSV
                     YQRKKK"
     gene            complement(17700..18698)
                     /locus_tag="PLZ54_01935"
     CDS             complement(17700..18698)
                     /locus_tag="PLZ54_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HOJ65732.1"
                     /translation="MENIAYKALVVEEDGNRYSRKIKELTTDHLFSYGDVLIRVYYSS
                     LNYKDALSATGNKGVTKNYPHTPGIDAAGVVIASNNEKFQSGEKVIVTGYDLGMNTPG
                     GFGEYICVPSDWIVKLPEGLTLKESMIIGTAGFTAGISLTRLTELVSPDMGEVIVSGG
                     TGGVGSMSLSMLAKLGYQTVAITGKTTEKSFFETIGCHKILPRSEFESLPQKPILSAK
                     YAGGIDTVGGGILENIIKQIQPLGAVTTCGSIHSTELNISVFPFILRGISLIGVSAQN
                     YPMNLREKLWKKLAADWKPEQLLNLYTEISLPQLSEAIDNMLQGKLKGRILVNLTA"
     gene            complement(18772..19140)
                     /locus_tag="PLZ54_01940"
     CDS             complement(18772..19140)
                     /locus_tag="PLZ54_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOJ65733.1"
                     /translation="MAKEVAQNSGIIYNSLTNGSKIVGTITAENDFRIDGEVEGEIFC
                     NGKVVIGQKGYLKGSISCNNAEIIGTVEGEISVQDTLSLRQTAKVTGNVKINVLIIEP
                     GAIFNGQCCMNETDSIQESE"
     gene            complement(19583..19912)
                     /locus_tag="PLZ54_01945"
     CDS             complement(19583..19912)
                     /locus_tag="PLZ54_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008620281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOJ65734.1"
                     /translation="MDFKKMNVPSTTITRDINLIGEKVGNIYEAVVIISKRANQISLE
                     IKSELDKKLQEFSAMGDNIEEVFENREQIEISRYYEKLPKPTLIAIEEFLEDKIYYRN
                     PIKEKLK"
     gene            complement(19926..20732)
                     /gene="bamD"
                     /locus_tag="PLZ54_01950"
     CDS             complement(19926..20732)
                     /gene="bamD"
                     /locus_tag="PLZ54_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOJ65735.1"
                     /translation="MKKYFLVFSILIFLFSSCSEYQKLLKSTDIELKYNKAIEYFNNG
                     DFARATTLFEDISSYYRGTDRSEDILNFLGQSYLGQKDYYTASNYYQTYLKSYPKGRY
                     AENAKFLVGYCYYKDSPDPRLDQTATYQAIASFQEFLDLYPESNKVPQVNKLLDELHD
                     KLAYKYYMNARLYYNLGNYMGNNYLSAVITAQNALKKYPSSSYRDDLSFLILQAKFAQ
                     AQQSILAKKEERYQDTIDEYYSYINEFPNGKFKKQANNIFNDCKKAINLK"
     gene            complement(20823..21812)
                     /locus_tag="PLZ54_01955"
     CDS             complement(20823..21812)
                     /locus_tag="PLZ54_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOJ65736.1"
                     /translation="MFLIATGIFFLIKNQSSSKYIFNEGKIHGTYYHAIYMHPQGIDL
                     QEKIEERMKKIDMSLSTFEPQSVISRINRNDDSVLTDDDFETVFKTAQKISEITYGAF
                     DITVAPLVNAWGFGFDSHDRILQPNLDTIMPFIGYQKIRLENHKLIKDYPKIMLDASA
                     IAKGYSTDIAANVLEENGCKNYLVEIGGEIVCKGKNTKGEKWKIGIDKPIDDETNTLN
                     EIQTVISITDKAMATSGNYRQFYIKDGKKYSHTIDPRTGYPVNKNILSATVIAPNCIT
                     ADALATAFMVLGVDSSLMICRTLPEVDCYLIYNDQKGSNKVIYSEGFENYFSK"
     gene            complement(22061..22630)
                     /locus_tag="PLZ54_01960"
     CDS             complement(22061..22630)
                     /locus_tag="PLZ54_01960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ65737.1"
                     /translation="MEKEYFVHESSYIDEQVKIGKGTKIWHFCHVQKGAEIGENCSLG
                     QNVNIANNVKIGNNVRIQNNVSVYEGVELEDNVFCGPSCVFTNVKTPRAHYPVHGIYD
                     KTLIKNGASLGANSTVVCGHTVGRSAMVAAGAVVTKNVKDYALVAGIPARQIGWVCEC
                     GERLEEDLVCKKCGKKYQETENGLKEIYL"
     gene            complement(23003..23959)
                     /locus_tag="PLZ54_01965"
     CDS             complement(23003..23959)
                     /locus_tag="PLZ54_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ65738.1"
                     /translation="MNKKICVVGGGKWGQNHIKTLYQLGNLGAIVEANAERLNELLKL
                     YPVEGFTELEQALEQDFDGFTVAVPAPLHYEVAKKIIESGKSVLVEKPMTLSAETSKE
                     LVELAQRKGVQLMVGHVLLFHPAIRKIKEIIDSGELGNLYYLYSNRLNLGTVRTEENV
                     FWSFAPHDISVLDYFVGHPAEKIVSKGSKFLQEEVYDVTITQLDYPNNVHAHIFVSWL
                     HPFKEQRLVVIGSKGMVSFDDATAEKEIKFYDKRIDFEKGIPVKVENPTRIIPYEQKQ
                     PLSEELKYFIEHLDKKIEIADGQSGLEVVKVLEEAEKKIQVG"
     gene            complement(23967..25280)
                     /locus_tag="PLZ54_01970"
     CDS             complement(23967..25280)
                     /locus_tag="PLZ54_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOJ65739.1"
                     /translation="MKNELLKKIEDRKIVVGVVGLGYVGLPLAVEKAKAGFKTIGFDV
                     QPQKVEMVNAGHNYIGDVVDSDLDELVKKGMLSATTDFSFVKDVDFIAICVPTPLDEH
                     QQPDISYVKSSAEAIGKFLKKGTIVVLESTTYPGTTEELIKPILEEESGLKCGIDFYL
                     GFSPERVDPGNLIYKTKNTPKVVGAIGEDALEVIAAMYRAVLEGDVFTVSSPAVAEME
                     KILENTYRNVNIGLINELAMLCHKMNINIWEVIEAAKTKPYGFQPFYPGPGLGGHCIP
                     LDPYYLSWKAREYGFHTSMIESSMMINDRMPEYCVERASKILNRSKKALNGSNILVLG
                     IAYKQDIDDYRESPALNVIDLLKSEGVNVCYFDPYIPEYLRHGKVFKGEKELTSALLA
                     KMDLVIVTAAHSNVDYDFVQQHAKYVFDTKNAMKNVKNRTNIEIL"
     gene            25700..26641
                     /locus_tag="PLZ54_01975"
     CDS             25700..26641
                     /locus_tag="PLZ54_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOJ65740.1"
                     /translation="MWKDFFYFSKGQQVGIIVLLVLIFVVSVVRFALPYLIPSNSNAF
                     DTNFVAEAENFKKSLLSLDSIQQAERQRQFEERYAAYRLNYRKDFSKKENHYELFNFD
                     PNTADSATFVQLGLKSYVASNILKYRSKGGSFKNAEDFSKIYGIPSEKFNELLPYIQI
                     DEKFSNPPVNLGEKAVKENPTSLVELNSADTTLLMQVKGIGKYYAKSIVRLRQNTGGF
                     FSVDQLSEIYGMTPENLEKIKPFCTVNPNLIEKIKVNTSSVDRLNKHPYINFYQAKAI
                     YELRRKKGKLHQFGELKNLSEFTEEQLKKIEPYLSFE"
     gene            complement(26848..27693)
                     /gene="lipA"
                     /locus_tag="PLZ54_01980"
     CDS             complement(26848..27693)
                     /gene="lipA"
                     /locus_tag="PLZ54_01980"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOJ65741.1"
                     /translation="MNFQRKPDWIRTKLHSEATFSEVQQIIEKNGLHTICTSGRCPNQ
                     SECWNKGTATLMILGEICTRACRFCNTLTGQPLPPDTTEPERIAQSIRSLHLRHAVIT
                     SVDRDDLPDKGASHWAKTIRAVKIVNPAITLETLIPDFDGETTLLDLVIAEKPDIISH
                     NLETVSRLTPKIRSKAKYQTSLKVLQYVSSCGITAKTGIMVGLGETEDEIFSLMDDAL
                     KAGCSVLTIGQYLQPSRKNIPVHEYISPKKFEEYRQIGLAKGFRHVESGPFVRSSYHA
                     EKHVL"
     gene            complement(27718..28407)
                     /locus_tag="PLZ54_01985"
     CDS             complement(27718..28407)
                     /locus_tag="PLZ54_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOJ65742.1"
                     /translation="MEILYEDNHIIAVNKTVSEIVQGDKTGDEPLSETLKKYLKNKYN
                     KPGNVFLGVTHRLDRPVSGVVLFAKTDKALSRLNEMFQNKEIKKTYWAIVQQKPPKMV
                     DTLEHFLVRNEAKNKTTAFDRPVDNAKKAILHYKVLTSSDYYYLLEIQLETGRHHQIR
                     CQLAKIGCPIRGDLKYGFPRPNSNGGISLHARKIEFVHPVSKNWIKIVAPAPQDDKLW
                     QFFEESQKEKE"
     gene            complement(28460..29230)
                     /gene="rlmB"
                     /locus_tag="PLZ54_01990"
     CDS             complement(28460..29230)
                     /gene="rlmB"
                     /locus_tag="PLZ54_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOJ65743.1"
                     /translation="MTIYKNKRRPEKEMIFGIRPVLEAIQAGKDIDKILLRRDMSNEI
                     SRELFAATKGLHIPVQFVPLEKLNKFTDKNHQGVIAFTSPVTYQRIEDIIPAVYEVGK
                     MPFIVVLDGITDVRNFGAIARTCECAGVDAIVIPAKGGVSITSDAVKTSAGALLSIPV
                     CRENNLYETLQFLADSGIKLIAATEKGEKNYTAIDFNEPTAIVLGSEDEGISPELLQL
                     CNEKVQIPIFGNISSLNVSVAAGVLIYEVIRQRKPKNQ"
     gene            complement(29254..>29675)
                     /locus_tag="PLZ54_01995"
     CDS             complement(29254..>29675)
                     /locus_tag="PLZ54_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65744.1"
                     /translation="LITSKMPTEDYLLKYLEKLSRVIAAMLGFRDSGFPEDALKMADG
                     TYRELLNVDLKELTVEPTEKFSETIRKANYSASILDLLVQLTYETANAFEQNRQSAEA
                     HSFYRKTLELYRLLNEKDKTFSFEREMRIGEINEKLL"
BASE COUNT         9158 a         5463 c         5452 g         9602 t
ORIGIN      
        1 atatgtaaaa taaatttgat tttcatatcc acctttcaaa aaaacagaat taattcgatg
       61 aattaaaaaa aattttttag tcaattcctc ccaaaaaaaa tttttcaatt agaagtaaat
      121 ctatatttat gaaaaacaac aacgaatgtg ccacgatttt caaaaacagc aaaaatacta
      181 aacttttttg attaatttta tttcttgaaa taagccacaa atgaaaaaca gatgttttac
      241 ggttgaaggg atggtagagt taggagtttt gagttttgag ttcagagttc agagtaaaaa
      301 tttgattaat tatttgataa tcagcgaata aatattttgg tagagatgta gcatgctacg
      361 tctaaaaagt ttcgagtgag gagttttgag ttcagagtaa aaatttgatt aattatttga
      421 taatcagtga gtaaatattt tgtagagacg tagcatgcta cgtctaaaaa gtttcgggtg
      481 aagagttaag agttcagagt ataattttta ttaattatct gataatcagt gtgtaaatat
      541 ttgatagagc cattgcatgt aaaagctaac gaatttgaaa gtctatttga tttgttcatt
      601 cacagaaaat tatccttgat aaaaaatttt ctcatcctta aatcgttaac acaaaataat
      661 tataacgtaa gttcaacata aaaatatttt ataagcaatt ataaattaat ctgataaggt
      721 tgatgaaaag cggtattcac atgtttctaa aatcagttgt ttgaaatgag gtcttaatat
      781 tcataaaact atcatcaaca acaagaataa aacttattac cttattacct tattacctta
      841 ttaattatca attatttata taatttaaac atggaatcgt aatatcgaac tctctttatt
      901 acaaatattt aaataaaatg aaacggattg gaattctttc ggacacacac ggttatttgg
      961 atccgcgtat atatcaatat ttttccgaat gcgacgaaat ttggcacgcc ggcgattggg
     1021 gttctttgga agtagtgaat cagctgaacg agctcaaacc tgtacgtgga gtatggggaa
     1081 atattgatga tgtaaatatt cgctcacttt ttccgcaaca ttttcatttt caatgcgaag
     1141 atgtggcagt atggctcaca cacattggcg gctatcccgg aaaatacgat aggtcggttc
     1201 gccctgctat tcttcaacaa cctccggatt tatttgtatg tggccattct catattttaa
     1261 aagttcagta tgacgaaaaa ctcaacttgt tgctcattaa tcccggagct gctggtaaat
     1321 atggttttca tacggttcaa accatcgtac gtctcgaaat cgacggaaaa aaaattcaaa
     1381 atcttgaagt aatcgaactg gaaaaataag aacttgatta actttgcata ctgtttattt
     1441 tgcaatcgaa gcaaaaaaaa ttgaatacta tgtttcaagt gaatgatacg cttatcagtg
     1501 agaatttatt ggaggaattt tttgtttgca atttggtttc ctgtaaaggt atttgttgcg
     1561 tggaaggcga tgctggcgct cctttggaaa aagacgaatt gaaaataata aaagatattc
     1621 ttcctgaaat tcaggaattt ttaccggaaa attcaaaaaa aataattcga gaaaaaggtt
     1681 tttattatat cgatcaggat ggcgattgcg taacctctct ggtgaatgaa agagaatgtg
     1741 tttttgcctt ctttgaagag gatggaactt gtaaatgtag tttggagaaa gcttattatc
     1801 agggaaaaac cgatttttgt aaaccgattt cctgtcatct ctatcctgta agactgcaaa
     1861 aaatcaacga ttacacggcc gttaatctgc atcactggaa agtttgcgaa tgtgcttttg
     1921 ttttgggaaa aaagttgaaa attccggcct atcgtttttt aaaagtccct ttgatacgtc
     1981 gttttggtga aaaatggtac aaagaagtgg aattgatagc cgacttgtta aaaaaaacaa
     2041 gagagagaaa tgattgagta ttggaaattc tctgtagaat aagcagcttg gaggaagtag
     2101 tgcgagatac gagattaaaa ttttgactaa ttatttgata atcagcaaat aaatattcag
     2161 tagagacgta gcatgctacg tctaaaaaat ttaaagttaa acgtgaaaat tttttgataa
     2221 tcagtatttt gttattatgt tgagccgttg catgaaacag ctcaagcaaa aattagacat
     2281 gaaagatatt ttttactgac aaaaaatata cataaaatta tacagaaaac atgaacagca
     2341 aaaaagtaga aatcataacc ataggagacg aaattcttat cggtcaaatc gtggacacca
     2401 actcggcatg gatggctcaa gagctcaaca aggctggctt tgaagtattt caaattactt
     2461 ctgttcacga cgaagcctcc catattacca acgctctcga tgaagcttta aaacgaacgg
     2521 atattgtgct gataacgggt ggcatcggcc caaccaacga tgatattacc aaaaaaacat
     2581 tgtgcgaata tttccatacc aagttggtgt tcgatccttc ggtaatagaa aatatcaaac
     2641 aggttttcgc caaccgaatc gattttgttt tcaacgattt gacttactcg caagctatgg
     2701 tgcctgaaaa ttgtaccgtc attcaaaaca aagttggaac tgcacccatt acatggtttg
     2761 aacaagacga aaaagtggtg gtttccatgc ccggtgttcc ctatgaaatg atggaagcca
     2821 tgcgtaacga aattattcca cgtttacaga aaaaatttca aacacaaaaa attctgcaca
     2881 aaacgattca ggtttttgga tattccgaat cggctttggc cataaaactt gcagactggg
     2941 aaaaagaatt gcccgatttt attcatttgg cctatctgcc gaattatgga atcgtcaaac
     3001 tcagactgac aggcacatca gacgatttgc tttcattgga atttgctgtc aatcagcaaa
     3061 tcgaatcttt gaaacaactt ttgggaaaag ccatcatcgc atttgaggat ttacctatag
     3121 agcagattgt cggaaatttg ctgacagcac aaggcaagac tactgccaca gccgaaagtt
     3181 gtacaggtgg caacatagct catcgcttta cttctcatgc gggaagttcg gaatatttca
     3241 aaggcggaat tgtagcttat gccaatgaag ttaaaacgga agttttgcaa gtatcctccg
     3301 aagatttgaa cttatatgga gctgtcagcc ggcaggtggt agaacaaatg gcaagaggag
     3361 ccttgcagct tttgcacacc gacacagcca tagccacttc cggtattgcc gggccttcgg
     3421 gcggaactgc ggaaaaaccg gttggaacag tttggatagc tgcctgctca aaagataaaa
     3481 tgacaagccg actttttcat tttggcaata cccgcgaaca aattattcaa cgttccacgc
     3541 aggctgcact ggtcatgctt aaagaaatta cataaaatgc attatattac tgctcaactg
     3601 atacaaggta attgccgtgt ttgtgtgtgt ttgaatctga aaaaatcact gattcttaat
     3661 ttttcatgtc aatttttttc acgaaaaacc ataaacctgt tgggtcttgg gtacaaacca
     3721 taaaaaaaga aaataaaaat tcataaaaaa attatctatt tatcaggaaa acagatattt
     3781 aattttaatt aaaaaaataa acaagaatta ttacaacaaa ttgcctttaa aatcaaataa
     3841 attgtaattt tttgattatt tttgcatcaa aatcactaca aaaaattaga aaaagcaatg
     3901 tttaaagtat cagaacgttt ggcagcctta tcaccttcgg aaacgcttgc catgtctcaa
     3961 aaaagtaacg aactgaaggc tcagggattt gacgttatca acctcagcgt tggtgaaccg
     4021 gattttaaca ctcccgatca cattaaagct gctgcaaaac aagcagttga caacaattac
     4081 tccttctatt ctccagttcc gggttttctc gaattgagaa aagctatttg ctcaaaactc
     4141 aaaaatgaaa acaacttgga atttaccccc gatcagattg tttgttccaa tggagcaaaa
     4201 caagccattt gcaatgtact gttgacaatt ttgaacaagg atgatgaagt gattattccg
     4261 gctccatatt gggtaagcta tcccgaaatg gtcaagttgg gcgaaggtaa atcggtattt
     4321 atctattccg acattgaaaa tgattttaaa atttctccgg aacaattgga aaaagccatt
     4381 acggaaaaaa ccaaagccct gattctttgt tctccttcca atcctactgg cagcgtttac
     4441 accaaagaag aattgcaggg attgactcaa gtattggcaa aacacccaca aatactcatt
     4501 ctctccgatg aaatttacga gcacatcaat tatgttggga aacacgaaag tattgcccaa
     4561 tttgaagaaa ttcgagacag agtggtagtt ataaacggtg tttccaaagg atacgcaatg
     4621 acaggctgga gaataggttg gatagccgct ccgaaatgga ttgcttctgc ctgcggaaca
     4681 ttacaaggac aatatacttc cggagcttgt tccgttgctc aaaaagccgc cgaagccgcg
     4741 tataccggag atcagtcgtg cgtggaaacc atgcgtgctg cctttgaacg acgtagaaat
     4801 ttggtggtgg aactggttcg ccaaataccc gggttgaaag ttagtgttcc acaaggtgca
     4861 ttctatgttt ttcccgattg cagtgcctat cttggaaaat ctttcaacgg aaaggttatt
     4921 gaaaattctg cagaccttgc catgttttta ctcgaaacag ctcatgttgc atcggttggc
     4981 ggtgtagcct ttggtgctcc gcaatgtatt cgcttttcct atgcaacatc cgaagataat
     5041 ctgaaaaaag cttttgaaag aattcagcag gcactcaaca aacttcaata atttttttaa
     5101 tttacaaata cgctgaaaaa cctttttatt ctttgtttgc tcaattaatt ttatcctttt
     5161 gttcctccca ctatggaaaa gaacaaaagg atattttttt ggaaaaaatg caaaaaagaa
     5221 aaactaaagt tccgccaaca cattcttcag caaaatcaag tatatttgtc tgaaaatttc
     5281 ataaatttct tttatgtgaa aacctattta tatttcattt agtcatgatt caatacttta
     5341 ccgaaaacat tccgatgccg gcactgaaaa aacaaaaaat caaccgctgg ataaaacaga
     5401 cagcccacaa ttacgaaaaa aaagttggag aaattgccta tatattttgt aacaacgaac
     5461 gcatcctcga aatcaatcga caatatttgc aacacgatta ttacaccgat atcatcactt
     5521 tcgacgacag tacggattcc acaatttcgg gcgatatttt tataagtttg gaaactgtga
     5581 aaagcaatgc cgaagatttc agtgtcgact ttgaagaaga gctccacaga gtcatcattc
     5641 acggtattct ccatctttgc ggacaagatg atattactcc cgaattaaaa gcagaaatga
     5701 ctcgaaaaga gaataaggca ctggaagaat taaaaaaaat gtaagttttg acgaaaaatt
     5761 tctaaaagtt gcatttttat ttcttggcat ctgaaaccat accaatgaaa gactaaaaac
     5821 ataaggagca ttcatacggt tggttatcag taaaataaaa tctttactct aaaacatttt
     5881 tcccctgctc cctaattttt gtcgttattg ttgtatattc cagaaatcaa taaataacac
     5941 aattttcaaa caaaatttaa ctcttgaatt agctgcttaa cctgcaaaaa atgccattgt
     6001 gattgaccct ctggatttgg aatatttcat tagaatataa tttatttttt aattgtctga
     6061 tggcatccac atgtcggaat attatattca ttcgtgtttg tatatgtaag acatgagggt
     6121 ttcatataaa aatcaatgat tgctaatctc ttccaaaaaa ataaatcagc ataattccga
     6181 acaaaataaa gaaaatctgc accatcgact ccttgaatga cgtttttctg tgcatttcag
     6241 gaatcaaatc agccaaagca atgtacaaaa aaccggcagc agaaaatgcc aatgcataag
     6301 gaatcaaaga cttggcaaaa ttgagcgaaa aatacgcaac tattccgaat atcaaagccg
     6361 aagctcctga taaagcattg taaagcaatg ctctggaacg tttcattccg ctttttaaaa
     6421 gtatcccgaa atcgcccaat tcctgtggta cttcatgaaa aattaccgct aaagttacga
     6481 cccaaccaaa agtattggaa gttaaaaagg ctgaagcaat aatcacccca tcaatggcat
     6541 tgtggaaggc atcgccaaat aaaaccattg gagccgctgt ttgaagattt ctttcgcatg
     6601 aagcgtcgga gcataccctc cataaaataa ttttttcgag aacaaagaaa aaaataattc
     6661 cagcaagtac cacatataac acctttttta tttccatcat gtcggaagct tcaggaatca
     6721 ttcccaacaa agctgatcca agtaatgtac ctcctgccaa gtaagttaaa acgtgtgtaa
     6781 ccctatgcaa ctgccgctct tttaacaaca aaattaaacc ggccaacagt acgctgccaa
     6841 cacttcctaa aaacaaagca acaacagtag aaagaaaact ttcggacata aattttattt
     6901 tttggtttac tacgaaataa atgaattact tatctaactt tctatgggta atagttcgtt
     6961 ttcatccgaa tacttttgat gtacatgagt ttcgccttct ttgaccaatt gataacagta
     7021 tttacaacga aaccattgat gctcaaactc aatatttccg cctttaatta ctatcatttt
     7081 tccttcaacc aatgcttggg caatggtttt tctggccgat tcgtaaattc tcgttagtgt
     7141 aggtcgcgaa acattcatgt gtccagctgc ctcttcctgc gtcattcctt cataatcgag
     7201 caaacgaatg gcttcaaacc catcaaaatg gagttcaatt ttatccaatt ccttatgtgg
     7261 aatgccaaaa ggcttgtaac ccatcataag cggaggcgat tttattatac gcttttgtcg
     7321 gggacgtgac atgtttttta tttttcaaac tacaaatata taaattattt tgaacttatg
     7381 ttcgttatta tttattttaa aaaaattcta ctaattaact cgcaattctt tcttcacatg
     7441 atgcttcatg tttgaattat cttttttaat ctatgaatat ttaaagaaaa tattttcatt
     7501 aaatttgcag caataatttt ccttaaattt aactgcttaa aaaattattt atggcatcaa
     7561 ctacaggcaa gtctgctcgt ttgctttccc ttgacgtatt gcgcggcatc accatcgccg
     7621 gaatgatttt ggtcaataat ccgggtaatt ggggaagtgt ttatccaccc ttgcgacatg
     7681 cagaatggaa cggcttaact ccaaccgatt tagtttttcc gttttttatg tttatcatgg
     7741 gtgtttccat gtatttatcc tacaaaaaat tcgattttaa attttctaat cgcacctttt
     7801 taaaattatt cagaaggtct tcattgctat ttttgattgg attggctatt ggctggctcg
     7861 gactgttcag tggcggatta cgtgaattgc aaggaacaga tataccattg ggtgaaagaa
     7921 tctggcaagc agccaccaat tttgaaaatg ttcgtatttt gggcgttttg caacggttgg
     7981 ctttggtatc tttttttggt acattgatca ttttgctgat tccgcacaaa cacattccct
     8041 ggttaacggg agcaatattg ttaatttact ggattctgat tggggtcacc ggaagttatg
     8101 ttgccagcga aaacaatatc attgcgattg tggatagagc tatactgggt gcttcacaca
     8161 tgtataccga aactttacct gacggaaaag ttattccctt tgatccggag ggaattttga
     8221 gcacattacc ctgcatagca catgtattaa ttggtttttg ggcaggaaaa ctgatttctg
     8281 aatcaaaatc gaaaaatgaa caaattcaaa acttggcaat ttttgggact atacttttct
     8341 ttgcaggttt ttttctccat tatggttttc ccgttaacaa gaaaatttgg tcttccagct
     8401 ttgttctagt aacttgtggc ttggcttcct tgcttttttc tttgctaatt tggattatcg
     8461 acgtaaacaa taaaaagaaa tggagtgtgt tttttgaatc ttttggggtc aatccattgt
     8521 ttatttatgt agttgccgat attttaagta ttttgctaag caatgttgta tttatgtatc
     8581 aaggcaattg gatttccatt aagaatttcg tgtattttga gtggttatct tctgtttttg
     8641 gaaattattt gggatcactc atctttgctt tatgttttgt tttcatcaat tggataattg
     8701 cacatatact ctacaaaaag aaaatttaca tcaaactttg ataggtgatt ttttttgaaa
     8761 atgtgataaa ttttcagtta aacaattgaa tgtcaataaa tcatttaaat tatctattaa
     8821 cttattccat tgaaaatttt attttatata acttaaactt atgaatttac tggaacaatt
     8881 aaaaaaattt acgactgtgg ttgccgacac gggggatttt gcatctatga tggcttatca
     8941 gcctgtggac gctacaacca atccttcatt gattttttct gcctctcaag atccaaaata
     9001 cgaatattta atggaagatg ctattcgcta tgcccaatct ttgacttctg ataaggaaga
     9061 acagttgaaa aaagcaatgg ataaattggc tgttaatttc ggtatcaaaa ttttggaaat
     9121 cgttccggga cgcgtttcaa ccgaagtcga tgcacgttta tcgttcgacg taaagggaac
     9181 aatagccaaa gcccgcgaat taattgctct atatgaatca gcaggaattt caagagaacg
     9241 aattcttatt aaaattgctt ctacttggga aggaattcgg gctgccgaaa tactggaaaa
     9301 agaaggcatt cactgcaatt tgactttgtt gttttcattg acgcaggctg ttgcttgcgc
     9361 tgaagctgga gtaacgttga tttcaccctt tgttggaaga attctggatt ggtacaaaaa
     9421 agatcgtgga gttaccgata ttccacctga agaagatcct ggagtaaaat ctgttacagc
     9481 catatacaat tattttaaaa agtttggtta tgccacacaa gtgatgggcg ccagtttcag
     9541 aaatatgggc gaaatcattc agctcgcagg atgcgatttg ttgaccattt cgccggcttt
     9601 gctcaaagaa ctcgagcaaa cggaaggtgt gctggaaagg aaattagatc cggaaaaagc
     9661 aaaaactgcc gacattcaac ctttgaaaat taatgaaatt agttttcgct ggatgatgaa
     9721 tgaagacgct atggctaccg agaaattggc cgaaggaatt agaaatttca ccaaagattt
     9781 gctgaaactc gaaaataaac tggccgcctt attgtgaaaa atggaattat tactgtccat
     9841 aaaaaagaag caagctcttt tttcgcaaaa acgaaagaga gcttgctaaa aatgaggtac
     9901 ttggcggatt cgaaccgccg tatgcggttt tgcagaccgg tgcctaacct ctcggccaaa
     9961 gtaccgtgtt ttggtctgca aagatagaac ttttttcctt cactccaaag ctttccgcag
    10021 aaaagtagct taataaaaaa cttagggacg gtttttttgc attttcctcc cctgaggcat
    10081 attatgaagc aatttgcgtt taaaagttcg ctccgcttga taataatgaa ataatttttc
    10141 gggtgaaagg gctttcttta attttagatg ataatttttg agtaactgtg cctgctttat
    10201 ttgcaaatta acatattcat cattcagcag ttcatagttt ggctgcgttt tattttcctt
    10261 cagaaaatta ttcaatgttt ctctgttttt ttcatgcaat tcccatacgg cattttcgta
    10321 attcataaat acaggtttca cttttgaggc ttcatcgggg gtcagttttg cctgttcaac
    10381 tatgtaagcc catttttttt cgtgcatttt ctggatattg gtgttggcgt tttcttctgc
    10441 tgaaagtgag tttacagtta tccacaccat tagcatcaaa atatatttga attttttcat
    10501 ttgaaattaa tttgaagtgt tttgaaaggt ttcagtacca ttttctgctt cataagcatc
    10561 attcatatag taatacatca tggttggttc gtccacttgt gagagcaaat acatttcgta
    10621 gttttcctgt ttttggcgtt cggtgttttt gaaaaccaaa taaaaaacat tgccgagcaa
    10681 aaatattccc acaaacatag cagccatata gagccatgtc ttcgaaataa cggcaaaact
    10741 tcttttttgc gatttggaaa cttccatttc ttttgcatag ccaatttgac gatcaatttc
    10801 attggcaaat tgctcgaaat aattttcagg aacgctgaaa ggtaagtcgt ttccaatttt
    10861 tttcaatatg tttttttctt ttttcataac tatttgttag actgtaaaaa atggtttagg
    10921 tttaatttgg aaatgttatt tagtacatca tctatcataa atcgataaaa atcaaattgt
    10981 tatattagtt gctaaatttc agtttcgctg tcgagtaaaa atttttcaat tttttttact
    11041 gccaaatgat aggatgcttt caatgctccg acagttgttc ccaatatgtt ttccatttgt
    11101 tcgtaagaga gatcatcgaa atatctcata ttgaaaacca gtctttgttt ttccggcaaa
    11161 gttaaaatgg cttgctgtaa ttttaattga gcttcgttac cgttgaaata ttggtcggct
    11221 tgcaactgtt gcagcaacac atcttccact tcgtcgagcg aattgatgtt tttcatcctt
    11281 ttgttggcca gaaaagtcag tgtttcgttg gtggcaatcc ggtaaaacca ggtagaaaca
    11341 agtgagtcgc ctctgaagtt gtctattccg ttccaagctt tcataaaagt attttgcaga
    11401 atatcgttag catcgtcgtg tgacagcacc atttttcgga tatgccaata caaacgttcc
    11461 tgatacatgc gtaccagttg cgagaaagct gcagcttttc ttttcgggtc ttgcagctgt
    11521 tctttcagtt tttcttcgcc ttttatattt ttatcgttct ccacttcaag ttggtcgggc
    11581 tactttttct tcattttgtt tgactttgat ttgtgccttt cgtttaattg accctggaaa
    11641 tatttttgtt gccctcaaaa aaacgcagaa tattttgtga ggcaaatctg ctcattgcgt
    11701 ttcgatcctc aatggtagct gttccgttat gaggcgaaag aacgacgtta tcgagttttt
    11761 tcaaattttc cggaattgtg ggttcatgtt caaatacatc gagaccggca ccaaaaattt
    11821 tatggttcgt taaagcttcc gtcaatgccg attcatcaat aaccgctcca cgtgaagtgt
    11881 tgattaaaat ggccgtaggt ttcatcaaac gaagtgtatt ttcattgatc agatggaaag
    11941 tgctttcggt caaaggtaca tgtaaactga tgacatcgct ttcacaaagc agttgtccga
    12001 gtggtgcata gcgagcttta agttttgttt ccgttttttt gtccaaagga tttcggttga
    12061 aatagataat tttcattccc aatgcagcag ctcttcgagc cacagctttc ccgatgcgtc
    12121 ccagcccgac aattcccagt gttttaccgt aaagtgagat gcccaaattt tccattactc
    12181 cccatttgat actgctgtct gttctcagtt ttctatcgca ttcgctcaca cgtcttgcaa
    12241 ctgtcagcat taaggcaatt gccatttcgg ctgttggttc aactacagga tcgggcgtat
    12301 tggttacaac aatgccgcat ttggtggcaa aatcaacatc aatattgtcg tatccgaccc
    12361 cataattggc aataattttc agttttcctt gagcgccggc gccaattacg tctcggtcaa
    12421 ccttaaattg aaaagttgga ataaacgcat caaactccgg aataatttgt atgacttctt
    12481 cttttgaaaa tatttctttt tcaggaaaaa caacttcaaa ttttttcatt aattccgaaa
    12541 aaccttcttc cggtaaacgg gttgaaacga gtacgcgcat attttttaga gaatagttgg
    12601 ttattgtaaa tggatggaac tcttggtctg atcatacaaa attacggaag tgtcaaaaag
    12661 tatgcaaata tataggttta aattatctat ttctttttga atacattgat tggatgacaa
    12721 ttggtcgtgg acaggaatta taaaataaat ttttttgtgt acttttgcga tgaaataata
    12781 aacttaaaaa agtaatatat tatggctgtt gtaaaacctt tcagaggaat ccgtcctcca
    12841 cgtgaaattg tagaaaaagt ggcttcccga ccttatgatg tattgaattc ggaagaagca
    12901 agagaagagg caaaaggcaa tccgatgtcg ctgtatcaca ttattaaacc ggaaatcgat
    12961 tttcctgaag gaacggatga acatgatgaa agagtatatg caaaagcagc agaaaatttc
    13021 aagaaatttc gtgaaaatgg ttggttggtt caggatgaag aagaacattt ttacatttac
    13081 gctcaaacca tgaatggccg tactcaatac ggaatagttg tttgcgccca tattgacgat
    13141 tacttgaacg ggcacatcaa aaaacatgaa ttgacacgca gcgacaagga agaagaccgc
    13201 atgaaacata ttcgtgttac caatgccaat gttgaaccgg tgtttcttgc ttatccttca
    13261 aatgaagaat tgaacggaat cgtttcggaa attgttaaat cagagccggt ttatgatttt
    13321 gttgcgcccg atggctttgg acatcatttc tggaaaatcg acgataaaga aattattcat
    13381 cgcatcacgg aaatttttca aaaaattcct tctctttata ttgccgatgg gcaccatcgt
    13441 tcagctgcgg ctgttcgggt gggcgatgag aaaagacgac aaaatcccca tcataccgga
    13501 aaggaagaat acaattattt tatggctgtt tgttttcccg acaaccagtt gcaaattatg
    13561 gattacaaca gggtggtaaa agatttgaat cagcttgacg atgaagagtt tctacaaaag
    13621 ctttcccaat attttattgt cgaaaaaatg ggtacagaaa tttatcgccc aaataaattg
    13681 cataattttt cgttatattt gtcgggaaac tggtattcgc tgacagctcg tgaaggaacc
    13741 tacaacgaca atgatcccat tgaaggtctt gatgttacta tctcttcaaa atatattttg
    13801 gataaaattc ttggaataag cgatttgagg actgataaac ggattgattt tgtgggagga
    13861 attcgtggtt tgggtgagtt gaaaaaattg gttgatagcg gaaaaatgcg ggtcgctttg
    13921 gcactttatc ccgtatcgat gcagcaactt attgccattg ccgacacggg aaacattatg
    13981 ccgccaaaaa ccacatggtt tgaaccaaaa ttgcgttccg ggctggtcat tcatgagctg
    14041 agctaaatta aaaaaacaat accggattaa attttttaat tttacggcat tacaaaattt
    14101 ttacgagttg ccgattaaca aatatttatg caatttttta taaaaaaacc gtcttttatt
    14161 ctctatcgaa ttatttcgct ttgtttgctt cttttatttc tgcaaagttg tggcgtcagt
    14221 tcacgtataa aaaaagccga ttcgcgattt gagttggggg aatattatgc tgcgggcgat
    14281 ttgtataaaa gttcgtatgg acggattccc aataaggata aatcattgcg tggatatgtt
    14341 gcttttcgac agggagagtg ttaccggctc atcaacaatc aacgggctga acaggcttat
    14401 ctgaatgcta ttcgaaacaa ttttgccgac agcaccgttt ttcttcggta tgcgcaagta
    14461 ttgcatcgaa atggaaaata tggcgaagct ttacgcaatt atcagctgta tttgcaaaaa
    14521 gattcttcct gcattattgc aaaaaatggt attcgtgcca ttcaaatgtt ggaggaatgg
    14581 cagaaaaatg caacccgtta tgttgtaaaa tatgataatc agtttaacgt tcgaagagct
    14641 tccactttca gtcccgcatt tgtaaataca gctgctgacg aactggtttt tacttctact
    14701 cgttctaccg ataaaaaagc cacagccaaa gccgatccca tcaccggttt acccaaaaac
    14761 aacctgtttc tggcgcgaaa aaatgcaact ggaaagtggg gaaaacccga attgttggaa
    14821 ggagaaataa atacacctga aaccgacgat ggcgtttgtt cctttgccgc taacggaaaa
    14881 accatttatt tcacacgggc tcagcaatcc aatttggtag atatgggtac gaaaatttat
    14941 gtatcttccc gagcaggcgc aacctggagt ccacctcaaa tgttgaaaat tttttccgat
    15001 agttcaattt cggttgccca tcctgccatt gcacccgatg gacaaacact ttattttgtt
    15061 tccgacaaaa aagatggctt gggaggaaaa gatatttata gagctacatt ggaaggaacc
    15121 gactgtaaat acattgaaaa tttaggtccg gaaatcaata cccccggcga tgaaatgttt
    15181 cccgttgttc gagccgatgg aactttgtat ttttcatcga atggacatcc aggatttgga
    15241 ggattggata tttttaaagc tgttccacaa aaaaatggag gatggaaggt tgaaaatatg
    15301 ggagcaccgg ttaattcaaa ggctgatgat tttgggatga cgtttgccgg agaaagcgaa
    15361 agcggttatt tttcttcaaa tcgcaacgaa atgagaggtt acgatgcttt gtggagtttt
    15421 catttgcccg aattgattta tttggttgaa ggaaaaatat ttgatgatca gggacaaact
    15481 atttctgatg ctacaattcg tattgttggc agcaatggga tgaatgctcg cattcaaaca
    15541 aaaaaggatg gtacttatcg aattaaactc gaaaaagatg tcgattacgt gatgctggca
    15601 accgctcgcg gttttctgaa tcaaaaaaat gaactttcaa cacaaggact taccgacagc
    15661 aaaactttca atgtaaattt caagctttca tctatcagta aaccggtgaa aattgaaaat
    15721 attttttatg aatttggaaa atgggatttg acgcctgctt cggaaaaatc tttgcaggaa
    15781 ctggttaaat tgctgaaaga caatccgaat attaccattg aattgagttc gcacaccgac
    15841 tacattggcg acaatgcttt caataagaat ctttccgaaa aacgggcgca gtctgtggtg
    15901 aattatttga taaaagccgg aatagacaag gagcggttga cagcggtggg gtatggagag
    15961 gaaaaaccga tagtggtaga tgaatcgctg gcccagaaat atccatttct gaaagtggga
    16021 gaaactttga cggaaagtta tatattaact ctcacccccg aacaacagga aattgccaat
    16081 caaattaatc gtcgtaccga attccgtgtt ttaaaaacta cttataattt gtattgatct
    16141 ttagcgcaga aaattattac aaatcgtaaa aagttgcatt attaacttga atttgttgct
    16201 tattaaaatt ttttgggatg aaacaatatt tggatttgtt gaatcgtgtt cttacagaag
    16261 gagttcataa agaagaccgt acaggagtgg ggactttaag tgtttttggt catcaaatgc
    16321 gttttccgtt gaacgaaggg ttcccttgtt tgactaccaa aaaattgcat ttaaaatcga
    16381 ttatttatga attgctctgg tttctgagag gcgatacaaa tgttaagttt ttgcaggaaa
    16441 acggtgtaag tatttggaac gaatgggcag acgaacatgg tgatttgggc ccaatttacg
    16501 gttatcaatg gcgctcgtgg cccgattatg aaggcggtta tattgatcag attgcccaag
    16561 cggtggaggc tatcaaaaac aatcccaatt cacggcgaat cattgtcagt gcatggaatg
    16621 tagccgattt gccaaaaatg aagttgcctc cgtgtcatgc tttctttcag ttttatgtgg
    16681 ccgacggcaa attgagcctt caactttatc agcggagtgc cgatattttt ttgggcgttc
    16741 cctttaatat tgccagttat gctctgctcc tgatgatgat ggcccaagtt acaggactga
    16801 aagctgggga ttttgttcat acgcttggag atgcccatat ttacaccaat catctggagc
    16861 aggtaaaatt gcagcttacg cgaactccac gacctttgcc aaccatgttg atcaacccgg
    16921 aggtaaaaga tattttttct tttcagtatt ccgactttca gctaattaat tatgaccctt
    16981 atcctcacat taaaggggaa gtagctgttt gaagttattt tctgaaaaaa tagtttaggt
    17041 tttacaataa gttttttata actttgtaaa tttgcatgtt tttgtgagaa ttatcaatat
    17101 ggattaaaaa ttacctatca tgcctaaaat ttcaattatt gctgctgttg ccgataatta
    17161 tgccatcggc aatttgaata aactgccttg gcatttgccg gccgatttga agtatttcaa
    17221 aaaacttact accggtcatg cagttatcat gggaaaacac acttttgaaa atttgcccaa
    17281 tgggcctttg ccaaaccgaa aaaatattgt gttaacttcg gtgttgtccg atggaatttg
    17341 tgaaggctat ttcgaagcca actccattga agaagctctc gaattgtgcg aaaaagaaga
    17401 cgaagttttt attattggcg gagtatccat ttttaagcaa accatcgatt tggcagataa
    17461 aatgtatatt acgtggatac atggagattt tccggcagat gcttattttc cgaaaattaa
    17521 ttttgaagaa tgggaagaaa tcagcagaga agatcatccg gccgatgaaa aaaatccata
    17581 tccttacaca ttttcagttt accagcgtaa aaagaagtaa ttttgcagca aattttttta
    17641 tgttaaccgg tcgtctatcg ttatttgact ttagacgact tttttattcg taaattttct
    17701 caggcagtca ggttgacaag aattctacct ttcagtttac cttgcaacat gttatctata
    17761 gcttctgaaa gttggggaag agaaatttcg gtgtaaagat ttaaaagctg ttcgggcttc
    17821 cagtcggcag ccaatttttt ccataatttt tcccgtaagt tcattggata attttgtgcc
    17881 gaaacgccta tgagcgaaat accacgtaaa ataaagggaa aaacggaaat attcaattcg
    17941 gtggaatgaa tgctgccaca agtagtaacg gcaccgagcg gctgtatctg tttgatgata
    18001 ttttccagaa ttcctccacc tacagtatca atgccaccgg catatttagc tgatagaatc
    18061 ggtttttgcg gcaaggactc aaattcgctg cgcggaagaa tcttatgaca accaatggtt
    18121 tcaaaaaaac ttttttctgt tgttttacct gtaatggcaa cggtttgata gccaagtttg
    18181 gcaagcatgg aaagagacat ggacccaaca ccacctgttc cacccgaaac gataacttca
    18241 cccatatcgg gagaaaccag ttcagtgagt cgagtcagtg aaattcctgc cgtaaatcct
    18301 gccgtcccaa ttatcatgga ttctttcagt gtcaaacctt caggaagctt tacgatccag
    18361 tcggaaggga cgcaaatgta ttctccaaag cctccgggcg tattcatgcc caagtcataa
    18421 ccggtaacaa taaccttttc gcccgattgg aatttttcgt tgtttgaagc tataactaca
    18481 cctgccgcat caattcccgg cgtgtgagga tagttttttg ttacgccttt gttgccggtg
    18541 gcagacagtg catccttgta attgagcgaa gaataataaa cccgaataag aacatcacca
    18601 tacgaaaaca aatgatcggt tgtcagttct ttaatttttc ttgagtatcg attgccatcc
    18661 tcttcaacaa ctaaagcttt ataggcaata ttttccattc ccaaattttg tttgatggta
    18721 aaaattgaat tgttgaattt cgattgtctt caggaaaata aagcgagttg tttattcgct
    18781 ttcctgaata gaatctgttt cattcatgca acattgtcca ttgaaaatgg cgccgggttc
    18841 tatgattagc acattaattt ttacgttgcc tgtaactttt gcagtttgac gtaaagacaa
    18901 tgtgtcttgt accgatattt caccttctac ggttccaata atttcagcat tgttgcacga
    18961 aattgatcct ttcaggtaac ccttttgtcc gatcactact tttccgttac aaaaaatttc
    19021 tccttccact tctccgtcaa ttctgaaatc attttcagcc gtgattgttc caacaatttt
    19081 acttccgttg gtcaacgaat tataaatgat tcccgaattt tgagcaactt ctttagccat
    19141 gtttcgaaat aaattttaat tgtttttttg ttcgcccaaa aatacgtgtt ttttttcaga
    19201 aatcaaattc caagcgtatg ggtcattcac cccactcaaa attttactct tttttgtttt
    19261 taatattatt tttttaactg aaatggaaag cgaagtttgg tggggagaga aacttaataa
    19321 gtgaaaatca aattgattga tgatagtaag gtaagctatt gagaaaatgt taagtacaaa
    19381 tcattcacct gcttctcaca tttaataaac ttattgaatg catctcgaaa atcttctaaa
    19441 ttcaactcaa tgacctcgtt tgttgtttgc tattagcgtg aagctattaa taacaaacaa
    19501 caaatgagga aaatatcgaa ataaatttaa atcaattttt ttgaaaaata ttcttcaaaa
    19561 acagtagttt aaggctgaaa ccctatttta atttttcttt tatcggattg cgataataaa
    19621 ttttatcttc caaaaattct tcaatggcaa tcaaggttgg cttgggtaat ttttcgtaat
    19681 aacgagaaat ttcaatttgt tcacgatttt caaatacttc ttcaatatta tcacccattg
    19741 ctgaaaactc ttgaagtttt ttgtctaatt ccgattttat ttccaaacta atttggttgg
    19801 cacgcttgct aataattact actgcttcat aaatattccc caccttttcg cctataaggt
    19861 ttatatctcg tgtgatggtt gttgaaggga cattcatttt tttaaaatcc ataaatattc
    19921 taattttatt ttaagttgat agcctttttg caatcattaa atatgttatt ggcctgtttt
    19981 ttaaatttgc cgttaggaaa ttcgttgatg tagctatagt attcatcaat ggtatcctga
    20041 taacgttctt cctttttggc aagaatactt tgttgagctt gagcaaattt tgcctgtaaa
    20101 attaaaaaag aaagatcgtc cctgtatgaa ctggaaggat attttttcaa cgcattttga
    20161 gcggttatta ctgccgacaa ataattgttt cccatgtaat ttccaagatt ataatacaat
    20221 cgggcattca tataatattt gtaagccaat ttatcatgta attcatcgag caatttgttt
    20281 acttggggta ctttattact ttcgggatat aaatccaaaa attcttgaaa tgaagctata
    20341 gcttgatagg tagcagtttg gtcgagcctg ggatcaggcg aatctttata atagcaatat
    20401 ccaaccagaa attttgcatt ttctgcgtaa cgtccttttg gatacgattt caagtaggtt
    20461 tgataataat tggaagcggt ataataatct ttctgtccta agtacgactg acctaaaaaa
    20521 ttcaatatat cttccgatcg gtcagttcca cgataataag atgaaatatc ttcgaataaa
    20581 gttgtagcac gagcaaaatc accgttgttg aaatattcga tagccttatt gtattttaat
    20641 tctatatcgg tgcttttgag caatttttgg tactcgctac atgaagaaaa aagaaatatc
    20701 agaatagaaa aaaccaaaaa atattttttc ataacaagca aatatattgc aaaagtaatt
    20761 attttactga gaataaaaaa atattttgat cagcttgcgg gaatgatcaa ctcctttggt
    20821 tattattttg aaaaatagtt ttcaaatccc tcgctgtata ttaccttatt actacctttt
    20881 tggtcgttat atatcaaata gcaatctact tccggtaatg ttctacaaat cataagactg
    20941 ctatcgactc ccaagaccat aaaagctgtt gctaaagcat cggctgtaat gcaatttgga
    21001 gctataacag ttgcgctcaa tatattttta ttgaccggat agcctgttcg tggatcaata
    21061 gtatgagagt acttttttcc atcctttata taaaattgtc tataatttcc tgaagttgcc
    21121 attgccttgt cggtgattga aatcaccgtt tgaatttcat tcagtgtatt ggtttcatca
    21181 tcgattggct tatcaatccc aattttccat ttttctcctt ttgtgttttt tcctttacac
    21241 acaatttcac ctcctatttc gaccaaataa tttttacaac cattctcttc gagaacattt
    21301 gctgcaatat ctgtggaata accttttgca atagcacttg catcgagcat gattttagga
    21361 taatccttta tcaatttatg gttctcaagc ctgatttttt gataaccaat aaaaggcatg
    21421 atggtatcaa gatttggttg cagaattcta tcgtggctat caaaaccaaa accccaagca
    21481 ttaacaagcg gtgcaacagt aatgtcgaaa gcgccataag taatttcaga aattttttgt
    21541 gctgttttga atacagtttc aaaatcatca tctgtcaaga cagaatcgtc atttctgttg
    21601 attcgggaaa ttacagattg tggttcaaaa gttgaaagag acatatctat ttttttcatg
    21661 cgctcttcaa ttttttcctg taaatcaatt ccttgaggat gcatatagat ggcatgataa
    21721 taggtaccat gaatttttcc ttcattaaaa atatatttcg acgaagattg atttttaata
    21781 agaaaaaata tgcccgttgc aattaaaaac aaaaagatta ttaaaaatat ttttctttta
    21841 acactcattt gtaaagatct tatttccttt tatttttaaa taggttttca attcatttaa
    21901 tgaaaactta actcaattag gactgatcat tcgctttaaa aaaaattaaa tttcatcaga
    21961 atataattta ttttttaaag gtctgatgga acccacctgt ctgaatatta tattcattcg
    22021 tgtttgtgta tgcaatacat gtgggtttca tatcaacaac ttataaataa atttctttca
    22081 atccgttttc tgtttcctga tactttttcc cgcatttttt acaaaccaaa tcttcctcca
    22141 atcgttcgcc acattcgcac acccagccaa tctgtcgggc gggaatgccg gcaacaaggg
    22201 cataatcctt cacatttttg gtaacgacgg ctccggcagc taccatagcc gaccgaccaa
    22261 ctgtatgtcc gcaaacgacc gtactgtttg ctcccagtga agcgccattt ttaatcaacg
    22321 ttttgtcgta aatgccatga acaggataat gagcgcgagg tgtttttaca ttggtaaaca
    22381 cacaggaagg cccgcaaaaa acattatctt ccagttccac accttcgtaa acggaaacat
    22441 tgttttgaat ccggacattg ttaccgattt tcacattgtt ggctatgttt acattctgcc
    22501 ccaacgaaca attttcacca atttctgcac ccttttgcac atggcagaaa tgccagattt
    22561 tggttccctt gccaattttt acctgttcgt cgatataact gctttcgtgt acaaaatatt
    22621 ctttttccat aaagtatttt gtttttattc aataatttaa gtctttcaat attgagtaat
    22681 gagtattgag tgtagaaaac agagtgttga gttttaataa aatgcaggag caataacaaa
    22741 ttaatttatt ataactcaca actaatacta ataataactt taaactatga actttaaact
    22801 caaaacttgt aactctttac tcctcactta ttacacaaaa ctccaaaata ttaactcttt
    22861 aaacgtagca tgttacgtct ctactgaata tttactcgct gattttcaaa tcattaataa
    22921 aaaatcttat ccaaatcttg caacgcttga ctcttgactc tttactctaa actcgaaact
    22981 cataactcaa aactactatc tcctacccta cctgtatctt tttctctgct tcttccagca
    23041 cttttaccac ttccaaaccc gattgtccat cggctatttc tattttttta tccagatgtt
    23101 cgataaaata tttcaattct tcggagagtg gttgtttttg ttcataagga atgattctgg
    23161 taggattttc aactttcact ggaattccct tttcaaaatc aattcgtttg tcgtaaaatt
    23221 taatctcttt ttcagccgta gcatcatcaa aggagaccat tcctttgcta ccaatgacca
    23281 ccagccgctg ttctttaaac ggatgaagcc acgatacaaa aatatgagcg tgaacattat
    23341 tcggataatc cagttgagta atggtaacgt cgtaaacttc ttcctgaaga aattttgaac
    23401 cttttgaaac aatcttttca gccggatgac ccacaaaata atccaaaacc gaaatatcgt
    23461 gcggggcaaa cgaccagaac acgttttctt ccgtacgaac cgtgccgaga ttcaacctgt
    23521 tggaataaag ataataaaga tttcccaatt ctccactgtc aattatctcc ttaatttttc
    23581 gaatagccgg atggaaaagc aatacatgac ccaccatcaa ttgaacgcct ttcctttgag
    23641 ccagttcaac aagttctttg gaagtctcgg ctgacaaagt catgggtttt tcgaccaata
    23701 ctgactttcc actttcaata atctttttgg ccacttcgta atgcagtggc gcaggaacag
    23761 caacggtaaa tccgtcaaaa tcttgttcca atgcctgttc caattctgta aatccttcta
    23821 cgggataaag tttcagcaat tcgtttaaac gttctgcatt ggcttcaacg atagcgccca
    23881 aattgcccaa ttgataaagc gttttgatat ggttttgtcc ccatttgccc ccaccaacaa
    23941 cacatatttt tttgttcatt tcaatgttac agtatttcta tattcgttct gtttttgaca
    24001 tttttcatgg cattcttggt atcgaaaaca tatttggcgt gttgctgaac aaaatcgtaa
    24061 tcgacatttg aatgagcagc agtaacaata accaaatcca ttttggccag caatgccgaa
    24121 gtaagttctt tttctccttt gaaaacttta ccatgacgca aatattcagg aatgtagggg
    24181 tcaaaataac aaacattcac tccttccgat ttcaacaaat cgataacatt cagtgccgga
    24241 ctttctcgat aatcgtcaat atcctgtttg taggcaatac ccaaaaccag aatattggaa
    24301 ccattcaatg cttttttaga gcgattgaga attttactgg ctcgttctac acaatattcg
    24361 ggcatgcggt cattgatcat catagaagat tcaatcatgg aagtatgaaa tccgtactca
    24421 cgagctttcc acgaaagata ataaggatcg agcggaatgc agtgtcctcc caaacccggg
    24481 ccgggataaa aaggctgaaa accataaggt ttggttttgg ctgcttcaat cacttcccat
    24541 atattgatat tcattttatg acacagcatg gcgagttcgt ttatcaagcc gatatttacg
    24601 tttcgatacg tgttttcgag tatcttttcc atttcggcca cagcagggct cgaaacggtg
    24661 aatacatcac cttccaacac agccctgtac atggcagcaa tcacttccaa tgcatcttcc
    24721 ccaatagccc caaccacttt gggcgtattt ttggttttat aaatcaaatt tccgggatct
    24781 actctttcgg gagaaaaacc caaataaaaa tcgataccgc atttcaatcc cgactcctct
    24841 tcaagaattg gttttattaa ttcttcagtt gttcccggat aagttgtaga ttccaacaca
    24901 acgatagttc ctttttttag aaacttgcct atggcttcgg cagagctttt tacataactg
    24961 atgtcgggct gttgatgttc atcgagcggt gtaggaacac atatagcaat aaaatcgacg
    25021 tcttttacaa agctgaaatc ggtagtagcc gaaagcattc ctttttttac cagttcatcc
    25081 aaatcgctgt ccactacgtc gccaatataa ttatgcccgg catttaccat ttcaactttc
    25141 tgcggctgaa catcaaagcc aatggtttta aaacctgctt tggctttttc cactgccaaa
    25201 ggtaaaccta cataacccaa accgactacc ccgacaacaa tttttctgtc ttcaattttc
    25261 tttagcaatt cgtttttcat atgctttatt taaaattgtc ataatttgta ttaaggaata
    25321 agaaaccaac atgtattaca tacacaaaca cgaatgaata taatattcag acatgtgggt
    25381 tccatcagaa ctttaaaaaa taaattatat tctgatgaaa ttaccttatt tttcagaaaa
    25441 ctcccatttc gttaagcatc cacaaagata atacaattca taaaaaaaag atgcctttgg
    25501 gaaaatttac aaaaattcac ttgttgaaaa gcactttcgg aaaatgacgg cttcacaaat
    25561 cagaaaatga aatatatcgc cgccgttttg gaataggaag aaagaaaaag acattcagtt
    25621 tgctgaaaat ttgaaaggtt aataaaaatt tttttaaatt tgcaacggtt tctaataaaa
    25681 tttactcaaa ttcagcacta tgtggaaaga ttttttctat ttttccaaag gacaacaagt
    25741 gggcattatt gttttactgg tgttgatatt tgtggtatca gttgttcgtt ttgctctccc
    25801 ctatctcata ccgagcaatt ccaatgcttt cgatacgaat tttgttgctg aggcggaaaa
    25861 tttcaaaaaa tcgctgcttt cgctggacag cattcaacaa gctgaacgtc aacgtcaatt
    25921 tgaagaaagg tatgcagctt acaggttgaa ctatcgaaaa gatttttcaa aaaaggaaaa
    25981 ccattatgaa ctttttaatt ttgatcctaa tactgctgat tctgctactt tcgttcaact
    26041 tggattaaaa tcttatgtgg cttcgaacat cttgaagtac cgaagcaaag gcggcagttt
    26101 caaaaacgca gaagatttct cgaaaattta tggtattcct tctgagaaat tcaatgaatt
    26161 acttccttat attcaaatag atgaaaaatt ttctaacccg cccgtcaatc tcggcgaaaa
    26221 ggctgtcaaa gaaaatccga cttcattggt agagttaaat tctgccgaca ccaccctttt
    26281 gatgcaagtg aaaggaatcg gtaaatatta tgcaaaaagc attgttcgac ttcgacaaaa
    26341 tacgggtgga tttttttctg tcgatcaact ttcggaaatt tacggaatga caccggaaaa
    26401 tctggaaaaa atcaaacctt tctgcaccgt caatccgaat ttgatagaaa aaataaaagt
    26461 caatacttct tctgtcgacc gtttgaacaa acatccctat atcaactttt atcaggcaaa
    26521 agccatttac gagttaaggc gaaagaaagg caaactgcat caattcggcg agctgaaaaa
    26581 cctgtcggaa tttactgaag aacaattgaa aaaaatcgaa ccttatttaa gttttgaata
    26641 aaaaatgatt tctatttggt ctgacgaact gatatcttgc gagaaaaaca tacttgttga
    26701 tttataaaaa agtaaaattt ttcatataaa ttaactgatt tccaatattt tatgatcaag
    26761 atgaaatcca aaaatatact gcaggacaat ggtaccctca ttacatttaa tgaataaccg
    26821 gaaaaatttc tttatggcaa agcgttttta caaaacgtgt ttttcggcgt gataggaaga
    26881 tcgcacgaaa ggtccacttt caacatgtcg gaatcctttt gccaaaccga tttgccgata
    26941 ttcttcaaat ttttttggag atatgtattc atgaacggga atattttttc gcgacggttg
    27001 caaatattgt ccaatggtca gaacggaaca acccgctttc aatgcatcat ccatcaatga
    27061 aaatatttcg tcttcggttt ctcccaaacc aaccatgatt cctgttttgg ccgttattcc
    27121 gcaggaagaa acatattgca gcactttcag gcttgtttgg tattttgctt tggaacgaat
    27181 tttaggcgtc aatcgactga cagtttcgag attgtgtgaa ataatatccg gtttttcggc
    27241 aataaccaaa tcgagcaacg ttgtttcgcc atcaaaatcg ggaatcagcg tttcgagggt
    27301 gatagccgga ttgacaattt tcaccgcacg aatggttttt gcccaatggg atgcaccttt
    27361 atcgggcaaa tcgtctctgt ccaccgaagt gatgacggca tgacgcaaat gcaatgatcg
    27421 tatgctttga gcaattcgtt caggttctgt agtatcagga ggcaatggtt gaccggtgag
    27481 ggtgttgcag aaacggcagg cacgtgtgca tatttctccc aaaatcatga gcgtagccgt
    27541 acctttattc cagcattcac tttgattggg gcagcgtccg ctggtgcaga tggtatgcaa
    27601 tccgtttttt tcgattattt gttgcacttc cgaaaaagtt gcttcgctat gtaatttggt
    27661 ccttatccaa tcgggttttc gctgaaagtt catgtttgtt gggatgaatt gtaaaaatta
    27721 ttctttttct ttttgcgatt cttcaaaaaa ttgccaaagt ttgtcgtctt ggggcgcagg
    27781 agcaacaatt tttatccaat ttttcgagac gggatgaacg aattcaattt ttcgggcgtg
    27841 caaactgatg cctccattgg aattgggacg cggaaaaccg tatttcaaat cgccacgaat
    27901 aggacagccg atttttgcca actgacagcg aatttgatga tgtcgcccgg tttcaagttg
    27961 aatttcaagc aaataataat agtcggaact tgtcaacacc ttgtagtgaa gaatggcttt
    28021 ttttgcatta tctaccggcc gatcaaaagc agtggttttg ttcttagctt catttctcac
    28081 caagaaatgt tccagcgtat cgaccatttt gggcggtttt tgctgaacaa ttgcccagta
    28141 tgtttttttt atttctttat tttgaaacat ttcattgagt ctggacaatg ctttatctgt
    28201 ttttgcaaac aatacgactc cgctaaccgg tcggtcgagg cggtgagtta ctcccaaaaa
    28261 aacgtttccc ggtttgttgt atttgttttt cagatatttt ttcagcgttt ccgatagagg
    28321 ttcatctccg gtcttgtctc cctgaacaat ttctgaaacg gtcttattta cggcaataat
    28381 gtgattgtct tcgtacaaaa tttccatgcg aaaataaagc tttttgaagc aaatattttg
    28441 taaatgtctt ggtagaattt tattggtttt tcggttttcg ttgtctgatg acttcgtaaa
    28501 tcaaaactcc tgcagctact gaaacgttga gagaggaaat gttcccgaaa atcggaattt
    28561 gaactttttc gttgcaaagt tgcaataatt cgggactgat gccttcatct tccgaaccta
    28621 aaacgatggc agtgggttcg ttaaaatcaa ttgccgtata atttttttct cccttttcgg
    28681 tggcagctat caatttaata cccgaatcag caaggaattg aagtgtttcg tacagattgt
    28741 tttcgcgaca taccggaatc gaaagcaaag caccggcaga agtttttaca gcgtcggaag
    28801 taatggaaac tccacctttg gcaggaatca caatagcgtc cacgccggcg cactcacatg
    28861 ttcgcgcgat agcaccaaag ttgcgtacat cggttattcc gtccaaaact actataaaag
    28921 gcattttccc gacttcgtac acggcaggaa taatatcttc gatgcgctga taggttacgg
    28981 gcgaagtaaa agcaataaca ccctgatgat ttttgtcggt aaatttattt aatttttcca
    29041 atggaacgaa ttgcaccgga atatgaagtc ctttggtagc ggcgaaaagt tcacgtgaaa
    29101 tttcattgct catgtcgcgt cgcaacaaaa ttttgtctat gtctttgccg gcttgtatgg
    29161 cttccaatac cggtcgaatg ccgaaaatca tttctttttc gggtcgtctt ttgtttttgt
    29221 agatagtcat gaaatttttt tttcaatttc taattaaaga agtttttcat tgatttcgcc
    29281 gatgcgcatt tctctttcaa aagaaaaagt tttgtctttt tcattcaata accgatacag
    29341 ttccaaagtc tttctgtaaa aactgtgggc ttcggcagac tgtcgatttt gttcaaaagc
    29401 attggccgtt tcgtaagtta attgaaccag caaatccaga atggaagctg aatagttcgc
    29461 tttacgaatc gtttccgaaa atttttctgt cggttcaaca gtcaattctt tcaagtcgac
    29521 atttaatagt tcacgataag tgccgtccgc catttttagt gcatcttcgg gaaatccgct
    29581 gtcgcgaaac cccaacatgg ctgcaataac gcggctcaac ttttcgagat atttcaataa
    29641 ataatcttct gtgggcattt ttgaagtaat cagtt
//
LOCUS       DAOSCH010000011        28485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1217_length_28485_cov_6.358319, whole genome shotgun sequence.
ACCESSION   DAOSCH010000011 DAOSCH010000000
VERSION     DAOSCH010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 28485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28485
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1217_length_28485_cov_6.358319"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..1034
                     /locus_tag="PLZ54_02000"
     CDS             132..1034
                     /locus_tag="PLZ54_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOJ65745.1"
                     /translation="MKKKFAILIFLSFCFSFHAYSQELNCNISINSDQVQGTNKSVFN
                     TLQKSVSEFVNNRKWTEMTFLTNERIECSMNIIVKSVDGDNFTAEIQVQSRRPVYNSN
                     YNTTLFNFKDNDFTFSYKEFEPLEWNEASISSNLTAVLAYYAYIIIGYDMDSFSRLGG
                     TPFFQAAENIVNSAQGSNLPGWRAFESSRNRYALANNLTDEAFKNFRNYFYEYHRLGL
                     DEMANNPVNARARIAQGISVLREANRARPSAVIISTFLDAKTDELINIFSKGTDREKA
                     LVVEILSDVNPTQTQKYEQILNKK"
     gene            1061..2719
                     /gene="recN"
                     /locus_tag="PLZ54_02005"
     CDS             1061..2719
                     /gene="recN"
                     /locus_tag="PLZ54_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004316862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOJ65746.1"
                     /translation="MLKTLHISNYALISEINIGFNSGFSVITGETGAGKSIIIGALSL
                     ILGQRAESRFIKEGEEKAIVEAEFDIRDYRLEHFFEINELDFHPEQCIIRREITASGK
                     SRAFINDTPVSLQLLRELSAKLIDIHSQHENLLLSDDQYQLQVVDNMAQNASILKQYQ
                     QYYSQWKNLQTQLSQLQKTADKQLSELDYLKFQFQQLEEAHLSENEQDDLEMELETLS
                     HTEEIKQELQKAVFLLTDEQKSLVLLKEVIDAVGRIKSYISEGENWHKRLESVYIELK
                     DIAQEITSLEENIDFHPDRLEWVDQRLNEIYTLEKKFKLSSISELLSLKENLKEELQH
                     IESFDEEIDALKKKIAEAHKNVHQQAELLTKSRLNAVEPIEKFLKEQLSRLGMPNIQF
                     KVEITDAAEFSETGKDTVQFLFSANKNQSLQPVAQIASGGEVSRLMLSIKSLMAHRAG
                     LPTIIFDEIDTGVSGEIAHRMAEIMKEMSKNMQVIAITHLPQIAAKGNQHYRVFKDDS
                     GKTTQTHINLLDREERIWEVAQMLSGENVTEVALGNAKELLKSE"
     gene            2784..3659
                     /locus_tag="PLZ54_02010"
     CDS             2784..3659
                     /locus_tag="PLZ54_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOJ65747.1"
                     /translation="MIQSMTGFGKATCEYEGKKIVVEIKSLNSKQLDLSLKIAAAYRN
                     KELEIRTEILQKLERGKIDVAIYIDNSGKDVANQINPSVFLSYMQQIKSLSQNLELPE
                     PTNWFDILLRLPDVMKTDNIELEENEWQALKKSIDKAIEQVISFRIQEGKSLENVFIS
                     KITNIGDLLEKISPYEAERIEKIRSRLKDNLQALSDKVDYDQNRLEQELIYYIEKLDI
                     NEEKVRLRNHLNYFFETMKLEPSAGKKLGFIAQEIGREINTLGAKANHSEIQKIVVKM
                     KDELEQMKEQVLNVL"
     gene            3724..4290
                     /gene="gmk"
                     /locus_tag="PLZ54_02015"
     CDS             3724..4290
                     /gene="gmk"
                     /locus_tag="PLZ54_02015"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOJ65748.1"
                     /translation="MSGKLIILSAPSGTGKSTLVQYLLTRPFDLEFSVSATSRPPRGS
                     EQNGKDYYFLSKEEFQKKIAQNEFLEYEEVYPGCYYGTLRSEIDRIAAKGKNIIFDVD
                     VLGGLNIKKQYNGRALAIFIAPPSLEELKNRLTIRGTDPPEMIAKRLEKAEFEMSFAP
                     KFDVVIVNDDLEKAKKATEEVVRNFLEK"
     gene            4304..4882
                     /gene="nadD"
                     /locus_tag="PLZ54_02020"
     CDS             4304..4882
                     /gene="nadD"
                     /locus_tag="PLZ54_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOJ65749.1"
                     /translation="MKKVGLYFGSFNPFHLGHQKLAEFLLDHQVVDEVWFVVSPRNPL
                     KKENDLLDENLRLEMVKIAIQDNPKLKVSDLEFSMPKPAYTIDSLHAFSSKYPENDFY
                     LLIGSDNAKVFDQWKNFKQILDEYPVLVYPRPGFDKEEIAKIYPQMKWLEGPQYNISS
                     TEIRQNIKQGKEVKDLLQPAVYQYIFKKKLYV"
     gene            complement(4885..6771)
                     /gene="ftsH"
                     /locus_tag="PLZ54_02025"
     CDS             complement(4885..6771)
                     /gene="ftsH"
                     /locus_tag="PLZ54_02025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOJ65750.1"
                     /translation="MTKRAKYSWIYWLVIMFLFILMDWLIFPTTQGNRELSYNEFVDS
                     VRNGKVERIIFLQDKIVGEFKSDTNHISSMTQAPTAPWRIRIPDLEEQVQKQFVVSRI
                     PNMNEEYLINDLLAAKVNFKGHFQDNTVLNFFLNWILPFVLFMLLWGYFFRKMGNKNP
                     MLEIGRNKAQIQAEKPANPITFKDVAGIDEAVEEVKELVDFLKNPSKYTRLGGKLPKG
                     VLLVGPPGTGKTLLAKAVAGEADVPFFSLSGSDFVEMFVGVGAARVRDLFNQAKAHAP
                     CIIFIDEIDAIGKSRAHSGYYAGGYDERENTLNQLLVEMDGFDASSGVIIMGATNRPD
                     VLDPALLRPGRFDRQVLVDKPDMNGREAIFKVHTKNIKLASDVDLKRLAAQTPGFAGA
                     EIANVCNEAAILAVRNNRDEVTMMDFEAAIERVIAGLEKKNKLINEKERKIVAYHESG
                     HAIVGYFTPGADEVQKVSIVPRGIGALGYTMQMPLEDRYLLSESELIGKIKGLLGGRA
                     AEELIFGEVSTGASNDLERATQLARNMITVYGMSKKLPNISLVNQSTTGFLGGGPGIE
                     RRSEYVEKMIDEEMTEIIRSCYENAKKLLSEKKDLLEKMAITLLEKEVLSYNEIKEIL
                     GEKN"
     gene            complement(6952..9177)
                     /locus_tag="PLZ54_02030"
     CDS             complement(6952..9177)
                     /locus_tag="PLZ54_02030"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOJ65751.1"
                     /translation="MENKVFFLVPVSAVLALFFAWYFFHQMKKESEGTISMQKIAKYV
                     REGAMAYLKQQYKVVGIVFLIITIIFSFMAYYLKIQNPWVPFAFLTGGFFSGLAGFIG
                     MRTATYASARTANAAQQSLNAGLRLAFRSGAVMGLVVVGLGLLDISIWFYVLQFVIGA
                     LDNATNLTIASDSSMKLIIITTTTLTFGMGASTQALFARVGGGIFTKAADVGADLVGK
                     VEENIPEDDPRNPATIADNVGDNVGDVAGMGADLYESYCGSILSTAALGAVAFAAKGK
                     AVQFNGVIAPMIIAAVGIILSIIGIFMVKSKEDASQKQLLSSLSRGINFSAIFIAIFS
                     FFILKYLNIPNYLGIWGSMLAGLFGGVAIGKITEYFTSAGFKPTQRISESSKTGPATV
                     IIAGIGTGMISTAFPVLVVGISIILSYLFASGFDFSNISMGLYGIAISAVGMLSTLGI
                     TLASDAYGPIADNAGGNAEMSGLAPEVRRRTDALDALGNTTAATGKGFAIGSAALTAL
                     ALMASYLEEIKIAIARIPEKTELFFNAMQTDIHQASIPQLIEYFQINLMNPRVLVGAF
                     IGAMSAFLFCGLTMNAVGRAAQKMVQEVRRQFREIAGIMEGKAEPDYARCVAISTKGA
                     QKEMLLPSLLAIIIPILTGVIFGVSGVMGLLLGSVSTGFILAIFMANSGGAWDNAKKY
                     IEEGNLGGKGSENHKAAVVGDTVGDPFKDTSGPSLNILIKLMSMVSIVMAGLTVAWSL
                     L"
     gene            complement(9251..10492)
                     /locus_tag="PLZ54_02035"
     CDS             complement(9251..10492)
                     /locus_tag="PLZ54_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOJ65752.1"
                     /translation="MSLVEILFYLILFFIVGDFILERYLSFLNFQYSKNELPDNLKDI
                     YDADKYGKQQEYFRSNIRFGTISSTINFIIILIFFCLGGFGWLDTLLRRITEHPILLP
                     LLYFGVLFVAETLISIPFQWYDTFVIEQKFGFNKVTPKLFVQDHLKEFALSVFLGGIL
                     LGAIVWIYQLTPKFFWLIAWSVVTLFSLFMSMFYSQIIVPIFNKQKPLEEGELRDEIE
                     KFATKAGFKLDNIYIIDSSKRSTKANAYFTGLGNKKRVVLYDTLLDKLTSEEIVAVLA
                     HEIGHYKKKHTLYQFLISLPESLLLFFLLGLILKSDVFAQVLGGTTASFHLNVIAFGL
                     LYTPVSLLLSICTNILSRKFEYQADAFAANFGWAKQLQSGLKKLAATSLSNLMPHPLY
                     AFFHYSHPTLYQRLTKLSGAQ"
     gene            10653..11612
                     /locus_tag="PLZ54_02040"
     CDS             10653..11612
                     /locus_tag="PLZ54_02040"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ65753.1"
                     /translation="MKRLQIFLFILITLFSLSCSNKTASDKISIAIPEGPSVVSFIKM
                     MDDSVSIEGKKVEYIVKSDPQQIQALMMQNKVDFAILPTNMAANLFNKGIKYKMIACP
                     VWGTLYILSNLPEVKTLNDLQNRKVSIFGQGTTADILFQKLIQKKGIEKVFIDYRFTG
                     NFELSQALRLKKTNIAVISEPLVSKLLEQDSTIHIVEKLSCEEYLNEKDRDIFVQTAF
                     LVNEKLERNNPQIIKEICNAYRASCNYVTQYPEEAAVLLVKHGILPETNVAKKSILLC
                     SINYVAAFAIEDEIYSYLKIFYDFNPESIGGKLPSENFIFQVF"
     gene            11624..13024
                     /locus_tag="PLZ54_02045"
     CDS             11624..13024
                     /locus_tag="PLZ54_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ65754.1"
                     /translation="MKNQNKKIFPFILSVFSLAIIWQIISISIGYPAIFPSIFDLISD
                     TLHLFTRSEFYFALLGTVSRGLLGFVITFFIALFFGSLSVFNSFFKDFFHPIVVFTRS
                     IPVISIVLVALLWFSPPFLPVFVAFFSMFPILFQSIQSGLENVDKKWVEMGKVFGLSS
                     RKRFLHIYYPAARSTIFDGISSAMGFGWRAVIIGEALAQPVHGIGSQMKAAQAYINVS
                     EIIAWTLAAILISYIFEKIILFLKKCNRPIQLPKSEECKLHKFNQIVDKKEITIYNLK
                     KKFNDYTVFSSFNENFKDTEINFIKGPSGIGKTTLLRLIARLEKPDEGTIYYSSKFSF
                     GYSFQDVRLLPWLTVKENIAFPLLYKKTDRQELTDNIDWLLNNMELTEHQNKFPDQLS
                     GGQLQRVGLARALVAQPDVLLLDEPFTGFDDDLKTRIQDFLSKWFEDFQPLVIWATHE
                     NILFPEKNRREIQLKN"
     gene            complement(13132..13653)
                     /gene="rimM"
                     /locus_tag="PLZ54_02050"
     CDS             complement(13132..13653)
                     /gene="rimM"
                     /locus_tag="PLZ54_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOJ65755.1"
                     /translation="MISFTEVIPIGQIIKLHGINGEMAFMSNYEGLDFDNIPFLIFDM
                     HGILVPFYLKEWRFNSENSGFLKIEDVNNEKKAKNFLGLTIYLHKKFLNILESDVDMD
                     NNTFIEFRIFDKDNNFIGIVTDIDTSTQNVLFIVDNGEKQILIPACQDYILQIDEMKK
                     IIYMDLPEGLLEL"
     gene            complement(13722..15422)
                     /locus_tag="PLZ54_02055"
     CDS             complement(13722..15422)
                     /locus_tag="PLZ54_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65756.1"
                     /translation="MAQDSVFTRNITVEREYQPILKNTGKLNVTPKLIEPEVKPLPAQ
                     YTELFIPLSINNNIHPLSSTEFFFNNRVEQNNFLQLGLGNFYNSSANFLYSLLNRPNT
                     KLDFYMNHIGTFGDKLHSTTKSGLVFDQQLKSLHLFAGVETQHERLKFYGFNFNDSAF
                     VQLNELAKNHGSSTFIERQFTSITRAPHSFTLQELSETPGTETFWHLNAFGGLQSLPL
                     TSDINYSAKLNYHFFQNKFGIKENRIHLTGNVIMPFHENNIGLDVQLHQIKYTANEAP
                     LFNFWDYYSVIMLNPFYSIEQTNWKLRMGLKAAFSMKHGRSFSPAADIAAEWNVFPQW
                     MTLYGGLSGDYEINSMDRIFSENCFLFSDTRIKDTYTPFLSYGGVKIKPAKNLLVDFY
                     ADYRFTDQQYFYVNKEYITEELNAPDSVLFTNRFNVAYSDAYHFKIGGRAAYNDEKNR
                     FNMEFRTTYNWWDTKDTALPWLQPTWETDLRANLGITSNITTSLYFFYKTGRYAQLGN
                     RSVKMDNIMDVNLGISYIVSKSLSVYLHLNNLLNNKYQYFYGYDVQGFNLMTGVAFSF
                     "
     gene            complement(15570..18614)
                     /locus_tag="PLZ54_02060"
     CDS             complement(15570..18614)
                     /locus_tag="PLZ54_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ65757.1"
                     /translation="MAKKNIPVLTLILFLCLSNLFLSAQHTLFFASEDKDFIQGKELY
                     FQQKYAASYRSFEQFLEKKTFPSDGQFQEAEYFLSANAYELRKENALSLLQTFLTKHP
                     YTPFSEEIYFMIGVLNCEQKNYTVARENFSQVNEQELSENKIPEFLFYKGYTAIQTQH
                     YEEAGKIFSRLKLQNSPFRVDATYYYAYSEYMMGNYQKALPDFLAIENHPDYQISVPY
                     FLVQIYYVEKQYAKLKEKADFLLQKYPNNKNNAEIFRILGELAYGEQNYAQAISYFKK
                     YENLSPKVLRNDVYLLGLSYFQLKDYSNAIQYLSKVTTEPDEISENAYLHLGTSYVFL
                     NDKTNARLSFEAALQTHFNPTVREEALFNYALTCYETTSAFGESVAAFTRFLSEFPNS
                     KYADEAYQYLTSVYLTTKNYELAYQSILKIRKLNTSLTETKQYLLYQMGVNAFAQKDY
                     GKAIEYFSLAHTSAPKGKYSAECLFWRAECYYRTNNPDQAIVDLETFIKNPLSRNSEN
                     LLYAYYSLGYGYFDQKNYIKALEWFSKYLENENSEKSMLYADALTRAGDCHFYARNFK
                     NALSFYEKAAFTSPNTADYSIFQAAYVEGLQKNYLNKIYRLEQLLLKYPNSEYGDDAL
                     YEIGRSYLMLKNEKMALSAYERLMNQYPSSVLSRKAALESGMIYFNRQDFENAIVIFK
                     YVAEKYPDTEESYIALKSMESAYIELNDVNGFLQYTKNSGRIVRPLSVMHEDSISYIA
                     AEKQYINGRYTVAISGFENYLNNFCPGGRYCTIAQFYLADSYYRTQQRDKALKAYEVV
                     LKTNAAEYVEQAALHCAEITYDQKDYASAFNYFKQLKQVAQNSANKQAAHLGMLRCSY
                     FTNDYQSLIDIANVILQDPYSEAIVKTEARFYRAKAYLLHQQLNAAIEDLKIISADTK
                     TAFGAESKYLLASVYFQQEKLTEAENEILDFAKKNTPHQFWLARSFVLLADIYIKKGN
                     DFQAKQYLLSLKRNYTIQDEIQGLIDERLSAISQREKKNIIH"
     gene            complement(18688..19230)
                     /locus_tag="PLZ54_02065"
     CDS             complement(18688..19230)
                     /locus_tag="PLZ54_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007219379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ65758.1"
                     /translation="MDFFSKFSFCPSCGSADFEINNEKSKRCKACGFVYYLNASAAVA
                     AFILNEKKELLVCRRKKDPAKGTLDLPGGFVDNYETAEEALKREIMEELQLEVENPTY
                     LFSLPNEYEYSNLLIPTLDLFFLCQIKKDLIPIPSDDVEDCFYVPLEKIQLELFGLKS
                     VKKAVEIFLREQIGKKCQNI"
     gene            19464..20831
                     /locus_tag="PLZ54_02070"
     CDS             19464..20831
                     /locus_tag="PLZ54_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ65759.1"
                     /translation="MGKKLLLPGDDEFKDLVHRYEQYLSGNANGYFDVEEFENIAEYY
                     LLRGRTKDSSRAIDFGMKLHPGSTALKAKRAKIYLAVDEVKKAYQLLSSFTESDYETL
                     LLKIEALVRMGRHAEADKLIKGLTTEKTADLDNIFLDIAYIYLENNKFEKASEFLLKG
                     FQINPKNIDLLFELAFSFEQMEEKEKAIEIYNRILDLDAFSGEAWFNIGQIYFNLQEF
                     PKALEAYDFVLAIDPNDSLAHLQKAHVHYQLEEFYTAIEYYAHYAAMTDDPASTSLYI
                     AECYEKLEQYDKAVDYYKYLLEQDPDNTDLLISIGSCLLEQEKFSASIEYLSRAIELN
                     DSLADAWVYLAEAYLAIDNLDIALKSYLLALQLDSEQPEVIMAVANIYLEKGDYETAL
                     NFYLAVEAMSNSLEIVNVFIAATYYKLGNKTEAYRYLKKGIEDNEDVLNLFLEICPEA
                     IETIH"
     gene            20931..21668
                     /locus_tag="PLZ54_02075"
     CDS             20931..21668
                     /locus_tag="PLZ54_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOJ65760.1"
                     /translation="MRLYGLIGYPLKHSFSKRYFAEKFRREGIDARYDLYELQRIDDF
                     PELVKMPGLCGLNVTIPYKESVIPFLNELDEVAEKIRAVNVIKFLRKNNHLWLKGYNS
                     DIIGFEKAIIPFLKTFHDKALLLGTGGAAKAISFVLAAHNIKVTHVSRKPGINKITYN
                     DLNEELMREHKLIINATPLGTYPNLCSCPDIPYEHLTPQHLLFDVVYNPPKTLFLKKG
                     EEQGATIINGETMLSGQAEAAWEIWNK"
     gene            complement(21814..22638)
                     /locus_tag="PLZ54_02080"
     CDS             complement(21814..22638)
                     /locus_tag="PLZ54_02080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOJ65761.1"
                     /translation="MKKTIISIMFGIMLLLPVFGTAQNNNSNKKSDIQTKSISYKIGD
                     QNFRGYLAWKDDGKKTKPGILVVHEWWGFNEYIKMRTDMLAELGYVAMAVDMYGDGKV
                     YDTAAEAQNAAGYIFSHPELLKERMEKGYEILTSEKTVDKKHVSAIGYCFGGTVVLNA
                     AAMGLPLETVVSFHGGLNGFKASPAMKNTKVLICNGAADMFVTQADIDNFKSEMQKYN
                     ITYEFKNYEGATHAFTNKYSTEAGKKFNMPIAYNEAADKASWQDMILFFNKYFPNK"
     gene            complement(22730..23458)
                     /locus_tag="PLZ54_02085"
     CDS             complement(22730..23458)
                     /locus_tag="PLZ54_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOJ65762.1"
                     /translation="MENKNNFVIKVEIWSDVVCPFCYIGKHKFEKALEKFPYRENVQV
                     EWKSFQLDPFAVTNPELKTMEHLAAEKGWSMEQALQSTAFITQMAKEVGLEFHFEKAI
                     VVNSFKAHRLLHLAKKHGKQNEMEEKLFAAYFTDGKNIDDNEILLQLAKEVGLEATET
                     NEVLLSDAYSDEVKHDIAHAHQLYVRGVPYFLFDDKFAVSGAQDESVFLQALNRALAE
                     HKKTDIIEFSGENGGVCGPDGCEI"
     gene            complement(23485..24447)
                     /locus_tag="PLZ54_02090"
     CDS             complement(23485..24447)
                     /locus_tag="PLZ54_02090"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOJ65763.1"
                     /translation="MTIIVTFKTFPEQKEYLKNVLGKEMQIYFKEDCTKEELENLMTK
                     ADVLLSWNPVQEGINKQNLPMDNIRFMQLLSAGYDHIRLEDFPEKLQIAANQGAYAEP
                     MAEHVMAMLLALSKRLYVYHNDLAKGNFSQIKSQTKFLKGSVAGIIGFGAIGKATARL
                     LRPFGVKLMATNSSGKTDEEVDFIGTLKDLDYVLQNADSLILSIALNEETEGLINKER
                     LELMKEDAVLVNVARGAIVEEAALYEHLKTHPNFYAGIDAWWVEPFKDRKFELHYPFF
                     ELPNLLGSPHNSAMVHNALLIGTEHAVQKILKFIHGEKVPGLIR"
     gene            complement(24459..25094)
                     /locus_tag="PLZ54_02095"
     CDS             complement(24459..25094)
                     /locus_tag="PLZ54_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HOJ65764.1"
                     /translation="MEQNKITIEFFHDVLCAWCYALSPRVEKLVEKYFDRIEVIHRSF
                     ALAPEPNGIEKIFGSKEEGKIQILGHWRAANMNDDAHRMHPELMATRTFDYPYSTRGL
                     LACKAAELQGGNEMHHKIFNRIQKAHLTECLNINDVEVLKMCAREVGLNVEQWEKDFH
                     TEKVKQMLNEDFYLAYQYGINSVPTLIANGKYKLTGAQPYETLEKWINQIK"
     gene            complement(25102..25920)
                     /locus_tag="PLZ54_02100"
     CDS             complement(25102..25920)
                     /locus_tag="PLZ54_02100"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOJ65765.1"
                     /translation="MKISEKLPVFFIPHGGGPWHVMDDFMGDPQGYGMLRNYLKKIGN
                     DFHDKIKAILVVSGHWEESVPTVNFGEHPSLLYDYYGFPEFTYQLSWPAPGDPQLALK
                     IENLLLEAGFTTARENLRGFDHGVFVPLMVAFPQADIPVVQLSLIKGLRPAQHIAMGK
                     ALESLRSEGVLIIGSGMSFHNLRGMMSGNQAISKISEQFDKWLTHAVENKNPEIRNQL
                     LTQWLSAPGARESHPRSEHLVPLFVVAGAAGNDLGKQDFSGTLLKVKISSYRFG"
     gene            complement(25968..26528)
                     /locus_tag="PLZ54_02105"
     CDS             complement(25968..26528)
                     /locus_tag="PLZ54_02105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOJ65766.1"
                     /translation="MIKIGIIVGSTRPGRNSEAVAKWVYEVASKRNDAIFEIVDIADY
                     NLPLLDEMIPASMHQYQKEHTKKWAEKINEMDAYVFVTPEYNHAPTAALKNAIDFVYA
                     EWNNKAAGFVSYGSAGGARAVEQLRQIMGEIQIADVRAQVTLSLFTDFENFSVFKPAS
                     SHEGSLHAMLDQLIAWGTALKTVREK"
     gene            complement(26536..26769)
                     /locus_tag="PLZ54_02110"
     CDS             complement(26536..26769)
                     /locus_tag="PLZ54_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65767.1"
                     /translation="METKKKLRCPLGIPGGIIAALIGLIGIIVNIVAFNWFQLIISLA
                     LLLLALPFVRVTMMVHMANDRLDELEEKINSKK"
     gene            complement(26785..27051)
                     /locus_tag="PLZ54_02115"
     CDS             complement(26785..27051)
                     /locus_tag="PLZ54_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ65768.1"
                     /translation="MNKKTTIELFYTLTCPNCKLTKQMLKEVLPQFEDKFSLKTTLAN
                     SPSGMIRTMKLGIHTVPTLLIDNKVVFKSVPTKEELIEKLKNYQ"
     gene            27567..28340
                     /locus_tag="PLZ54_02120"
     CDS             27567..28340
                     /locus_tag="PLZ54_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOJ65769.1"
                     /translation="MDLVGFFILGIAAGILSGLFGIGGGIVIVPSLIVIFGLDILDAN
                     AVSLAAMLLPVGILGVWAYYKEGYVNIGESLWVALGLFLGSFVGGGIAVNIGESLLAK
                     LYAGILIYIAISYFQLPKYFRKKQPIEKEEKPREKKSFWAFVLIGLAAGVFAGLFGKG
                     GGLVIVPMLIHFFHYDSRSAAATSLAALQLPVGLPSVLIYAENGHLNLLFAALIALGI
                     FVGVLIGSKIGIKIPPSIFKKIYAVFLLAIAVYMAFEYI"
     gene            complement(28386..>28485)
                     /locus_tag="PLZ54_02125"
     CDS             complement(28386..>28485)
                     /locus_tag="PLZ54_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_091910270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOJ65770.1"
                     /translation="YFGDAIFDRLLVAAVSYNTDFKSRIYNRNHCG"
BASE COUNT         9100 a         5252 c         5053 g         9080 t
ORIGIN      
        1 gccggatttg gctacgatac caataaaatt accattttgc ataataccgg caaaagcgtt
       61 tcctttgatt taaagccaaa aaaagaagtg gcggaagata tagttaatga gttggttaaa
      121 attttgaatc gatgaaaaaa aagtttgcta ttctgatttt cttatcattt tgtttttcct
      181 ttcatgctta ttctcaggaa ttaaactgca atattagcat taattccgat caggtgcaag
      241 gaacaaataa atctgttttc aacactttgc agaaatcggt ttccgaattt gtgaacaacc
      301 gcaagtggac ggaaatgact tttttaacca acgaacgaat tgaatgttca atgaatatca
      361 ttgtaaaatc ggttgatgga gataatttta ctgctgaaat tcaggtacag tcgcgtcggc
      421 cggtttacaa ttcgaattac aacacaactc ttttcaattt caaagacaac gattttacgt
      481 tctcatacaa ggagtttgag ccgttggaat ggaacgaggc atccatcagc tccaatctca
      541 ctgcggtatt ggcttattat gcctatatca ttatcggcta cgacatggat tctttttcaa
      601 ggttgggagg cacaccattt tttcaagccg ccgaaaatat agttaattct gctcagggaa
      661 gtaatttacc cggatggcga gcttttgaaa gcagccgaaa tcgttatgct ttggcaaata
      721 atttgactga tgaagctttc aaaaattttc ggaattattt ctacgaatat catcgtctgg
      781 gattggacga aatggccaat aatccagtta atgcccgtgc ccgaattgct caaggaattt
      841 ccgtgttgcg cgaagccaat cgtgcccgcc catcggcagt aataatttct acatttttgg
      901 atgccaaaac ggatgaattg atcaatatat tttctaaagg aacggacagg gaaaaagcat
      961 tggtcgtaga aattctgtcg gatgtaaacc ccacacagac acaaaaatac gaacaaattt
     1021 taaataaaaa ataaatactt tttctgacta ccacatattt atgcttaaga cgcttcatat
     1081 cagcaattac gcattgattt ccgaaattaa tatcggtttt aattccggct tttcagttat
     1141 tacaggcgaa accggtgctg gaaaatccat cataatcggt gcgctttcgt taatactcgg
     1201 acagcgggct gaaagtcgtt ttatcaagga aggagaagaa aaagctatag ttgaagccga
     1261 atttgatatt cgggattatc gtttggaaca tttttttgaa ataaacgaat tggattttca
     1321 tcctgaacag tgcattatcc gtcgtgaaat aacagcatcg ggaaaatcaa gagcttttat
     1381 caatgatacg cctgtaagtt tacagctttt acgtgaactc agcgccaaat tgatcgatat
     1441 acattcacaa cacgaaaatt tattgctttc agacgaccag tatcaattgc aagtggttga
     1501 caacatggca caaaatgctt cgattctcaa gcaatatcaa caatactata gtcaatggaa
     1561 aaacctgcaa acacagcttt cccaattgca gaaaactgcc gataaacaat tgtctgagct
     1621 cgattattta aaatttcagt ttcaacagtt ggaagaagcc catttgtcgg aaaacgagca
     1681 agatgacctt gaaatggaac tggaaacatt gagccacacg gaagaaatta aacaggaatt
     1741 gcaaaaagcc gtttttttac ttaccgatga acaaaaaagt cttgttttgc ttaaagaagt
     1801 tatcgacgct gtaggacgaa taaaaagtta catttcggaa ggagagaatt ggcataaacg
     1861 tttagagtcg gtttatatcg aattgaaaga tatagctcag gaaataacaa gtttagagga
     1921 aaatattgat tttcaccctg atagattgga atgggtcgac caaagattga atgaaattta
     1981 tacgttggaa aagaaattta aactgagttc tatttccgaa ttattatcgc tgaaagaaaa
     2041 tttaaaagaa gaattgcagc acatcgaatc ttttgatgaa gaaatcgacg ccttgaagaa
     2101 aaaaattgcc gaagctcata aaaatgttca tcaacaagcc gaattattga caaaaagtcg
     2161 gttgaatgcc gttgagccga tagaaaaatt tttgaaagag cagctttcac gtttgggaat
     2221 gccgaacatt cagttcaagg ttgaaattac ggatgctgcc gaattttcgg aaacagggaa
     2281 agacacggtt caatttcttt tttcggccaa taaaaatcaa tcgttgcagc ctgttgctca
     2341 aatcgcttcg ggaggtgagg tttcacgttt aatgctctcc atcaaatctt taatggcaca
     2401 tcgagccggt ttgcctacca ttatttttga cgaaatcgat acgggcgttt ccggcgaaat
     2461 tgctcatcga atggctgaaa ttatgaaaga aatgagtaaa aacatgcagg taattgctat
     2521 cacacattta cctcaaattg ctgccaaagg gaaccaacat tatcgtgttt ttaaagacga
     2581 ttcaggcaaa actacacaga ctcatatcaa cttgctcgat agggaagaac gaatttggga
     2641 agttgcgcaa atgctcagcg gtgaaaatgt tacggaagtt gcattgggca acgccaaaga
     2701 attgcttaaa agcgaataaa aaaaccggaa atttgaattt cggtaacata aacaattgca
     2761 aaaatcactt tttacaaaat actatgatac aatccatgac aggtttcggc aaagctacct
     2821 gtgaatatga agggaaaaaa attgtcgttg aaattaaatc actcaacagc aaacaactgg
     2881 atctttcttt gaaaatagcg gctgcatacc gaaataaaga actcgaaatc agaaccgaaa
     2941 ttcttcaaaa attggaacgc ggaaaaattg atgtggctat ttacatagat aactcaggca
     3001 aagatgttgc taatcaaatc aatccatctg tttttttatc ttacatgcag caaatcaaat
     3061 ctttatcaca gaatcttgaa ttgcctgagc caacaaattg gtttgatatt ttgcttcgat
     3121 tgccggatgt aatgaaaacc gataacatcg aattggaaga aaatgaatgg caagcactta
     3181 agaagagtat tgacaaagcg atagaacagg ttatttcttt ccgtattcag gagggaaaat
     3241 ctctggaaaa cgtttttatc tcaaaaatta caaatattgg cgatttattg gaaaaaattt
     3301 cgccttacga agcggaaaga atagaaaaaa tacgttccag actcaaagac aatctgcaag
     3361 cactttctga taaggtagat tatgatcaaa atcgtttaga gcaggaattg atttattaca
     3421 ttgagaaact cgatattaat gaagaaaaag ttcgtttgcg aaaccatctg aattatttct
     3481 ttgaaaccat gaaactggaa ccatcggcgg gcaagaaact cggttttatt gctcaggaaa
     3541 ttggtcgtga aatcaatacg cttggagcca aagccaatca cagtgaaatt caaaaaattg
     3601 tggtgaaaat gaaagacgaa ctggaacaaa tgaaggaaca agtattgaat gtcctttaat
     3661 ttgataaatt tcttttatta taaggtaaaa caattgtact acgtttaaaa tttcactttc
     3721 ttcatgtccg gaaaattaat tattttatcc gcaccatcag gcacaggcaa aagcacgctg
     3781 gttcaatacc ttctgacacg tccttttgat ctggaatttt cagtgtcagc taccagtcgt
     3841 ccaccgcgcg gaagcgaaca gaacgggaaa gattattatt ttctttctaa agaagaattt
     3901 cagaaaaaga tagcgcaaaa cgaatttttg gaatacgaag aagtttatcc gggatgttat
     3961 tatggaactt tgcgaagcga aattgatcgc attgcagcga aggggaaaaa tatcattttc
     4021 gatgtggatg tgcttggagg acttaatatt aaaaaacaat acaatggcag ggcattggcc
     4081 atttttattg ctcccccaag tttggaagaa ttaaaaaatc gtctgacgat tcgaggtacc
     4141 gatcctcccg aaatgattgc caaacgactt gaaaaagctg aatttgaaat gagtttcgct
     4201 ccaaaatttg atgtagtaat tgtgaatgac gatttggaaa aagctaaaaa agcaaccgaa
     4261 gaagttgttc gcaacttttt ggaaaaataa gttgtcccgc aaaatgaaaa aagttggatt
     4321 atattttggt tctttcaatc cttttcatct cggacaccaa aaactggccg aatttcttct
     4381 tgatcatcaa gtggttgatg aagtttggtt tgttgtttct cctcgcaatc cgttaaaaaa
     4441 ggaaaatgat ttgctggacg aaaatcttcg cttggaaatg gtaaaaatag ccatacagga
     4501 caatccgaaa ttaaaagttt ccgacctcga attttcaatg cctaaacctg catataccat
     4561 tgacagtctg catgcttttt cttccaaata ccctgaaaac gatttttacc tgctcattgg
     4621 tagcgataac gcaaaagtct ttgatcagtg gaaaaacttt aagcaaattc tcgatgaata
     4681 tcccgtgttg gtttatccgc gtcccggatt cgataaagaa gaaattgcca aaatctatcc
     4741 tcaaatgaaa tggttggaag gtccacaata caatatttct tcaacggaaa ttcgccaaaa
     4801 cattaaacaa ggtaaagaag taaaagattt gcttcaacct gctgtttatc aatatatttt
     4861 taaaaaaaag ctatatgttt aatgctaatt tttctctccc aaaatttctt ttatttcatt
     4921 ataactcaaa acttcttttt ctaaaagtgt gatggccatt ttctccaata aatctttttt
     4981 ttctgacaat aatttttttg cattttcata acatgagcga atgatttcgg tcatttcttc
     5041 atcaatcatc ttttcaacat attccgaacg tcgttcgata ccgggtccgc cacccaaaaa
     5101 accggtagtt gactgattta cgagggaaat attaggcaat tttttactca ttccataaac
     5161 tgtaatcatg tttcgagcca attgagtagc tctttccaaa tcgttggaag cgccggttga
     5221 cacttcacca aaaatcaatt cttcagcagc tcttccgccc aaaagtcctt ttatttttcc
     5281 tatcaattca ctttcagaga gtagatatct gtcttccaga ggcatttgca tggtatatcc
     5341 caaagctcct atacctcttg gaactattga aactttctga acctcgtctg ctcccggagt
     5401 gaagtatccg acaatagcat ggccactttc atgataagct acaatttttc gttccttttc
     5461 gtttataagt ttgttctttt tctccaaacc ggcaattaca cgctcaatag cggcttcaaa
     5521 atccatcatg gtaacttcat cacgattgtt tcgtacagct aaaattgcgg cttcattaca
     5581 aacgttggca atttcggcgc ctgcaaatcc gggcgtttgt gcagctaatc tctttaaatc
     5641 gacatccgaa gccaatttaa tattttttgt atgtacttta aaaattgctt ctctcccatt
     5701 catgtcgggt ttatcgacca atacctgtcg atcaaaacgt cctgggcgca acaatgccgg
     5761 atccagcaca tctggccgat ttgtggctcc catgataatt actccggaac tggcatcaaa
     5821 tccatccatt tcaaccaaaa gttgattcag cgtattttcc cgttcatcat aaccgcccgc
     5881 ataatatccc gagtgagctc tgcttttacc gatagcgtca atttcatcaa taaaaataat
     5941 acagggtgca tgagcctttg cctgattgaa caaatctcta actcgggcag ccccaacccc
     6001 tacaaacatt tcgacaaaat cggaaccgct cagactaaaa aatggaacat ccgcttcgcc
     6061 tgctactgct ttagccaaca aggtttttcc tgttcccgga gggccaacca gcaggactcc
     6121 ttttggtaat tttccgccca aacgtgtata ttttgaaggg tttttcagaa aatcaaccaa
     6181 ctcttttacc tcttcaacgg cttcgtcaat ccctgctacg tctttaaaag ttattggatt
     6241 tgcaggtttt tctgcctgaa tttgtgcttt gtttcttcca atttcgagca tggggttttt
     6301 gttgcccatt tttctgaaaa aatatcccca taacagcata aacaacacaa atggtaaaat
     6361 ccagttgaga aaaaagttta aaacagtatt gtcttgaaaa tgacctttaa aattaacttt
     6421 tgccgccaac aaatcattaa tgagatattc ttcgttcata tttggaattc tgctgacaac
     6481 aaattgcttt tgtacttgtt cttccaaatc aggaattctg attctccatg gggcagtggg
     6541 agcttgtgtc attgaggaaa tatggttggt atccgatttg aattctccaa ctattttatc
     6601 ctgtaaaaaa ataattctct ccactttgcc gtttcgtaca gaatcaacaa actcgttata
     6661 gcttaattcc ctgttccctt gtgtggtcgg aaaaataagc caatccatca aaataaacaa
     6721 aaacatgata accagccagt aaatccatga atattttgca cgttttgtca ttcaaataaa
     6781 tttttttagg tttataaaat ttcgacgaaa attagaaaaa aaaaacggga aactcaatga
     6841 aaaaccgatt ttattatttg atttttttca tttattatta ccaaaacaca aaaaatcccg
     6901 actttttaaa tttttgagcc gggattttta aaataattcg ttaaaaaaca attacaaaag
     6961 gctccatgct acagtgagac ctgccattac gatggaaacc atgctcatga gttttattaa
     7021 aatattcaag gaagggcctg aagtatcctt aaaaggatct ccaactgtat ccccaaccac
     7081 tgcggctttg tgattttccg aacctttacc gcccaaattt ccttcttcta tatatttttt
     7141 ggcattgtcc catgcgccac cggaattggc cataaaaatt gccagaatga aaccggtaga
     7201 aacacttccc agcaacaatc ccatgacacc cgaaacgcca aatatcacgc cggtaagaat
     7261 aggaataatg attgccaaca aggaaggaag caacatttcc ttttgcgctc cttttgtaga
     7321 gatggctaca catcgagcat agtcgggttc agctttgcct tccatgatac cggcaatttc
     7381 gcgaaattgg cgacgaactt cttgaaccat tttttgagcg gcacgtccga ctgcattcat
     7441 tgtcagtcca caaaacaaaa atgcagacat tgcaccaata aatgctccaa ctaaaactcg
     7501 aggattcatc aaattaattt ggaaatattc tattaattgt ggaatagaag cttgatgaat
     7561 atcggtctgc atggcattga aaaataattc tgttttttcg ggaatacgag caatggcaat
     7621 tttgatttct tccaaataag aagccatcaa tgccaaagcc gtaagggcgg cagaaccaat
     7681 ggcaaatccc tttcctgtgg cagctgtagt atttcccaaa gcgtccagtg catccgtgcg
     7741 tcttctaact tcaggagcca atccgctcat ttctgcattt cctccggcat tatcggcaat
     7801 agggccataa gcatcagaag ccagcgtgat tcccaacgta gaaagcattc ctacagccga
     7861 aatggcaatt ccatacaatc ccatgctgat attggaaaaa tcgaaacccg aagcaaacag
     7921 ataggacaaa attatagaaa tacccaccac caaaaccgga aaagcagtcg aaatcattcc
     7981 tgttccgata ccggcaataa ttaccgtagc aggtccggtt tttgacgatt cagaaattcg
     8041 ctgtgttggt ttaaaacctg ccgaagtgaa atattcggtt atttttccaa tggcaacacc
     8101 tccaaaaagt ccagccagca tcgatcccca aatgcccaaa taattaggta tattcaaata
     8161 tttcaaaata aaaaatgaaa atatggcaat gaaaatggcg ctgaaattga ttccacgact
     8221 taaagagctt aacaattgct tttgagaagc atcttctttg gattttacca taaatattcc
     8281 aataattgat agaataattc caacggctgc aataatcatg ggcgcaatta cgccattaaa
     8341 ttgaactgct tttccttttg ccgcaaaagc cacagctcct aaagcagcag tagaaagtat
     8401 cgatccacaa tatgattcgt acaaatcggc ccccataccg gctacatcgc ctacattatc
     8461 acccacatta tcggcaatgg tggcaggatt tcgaggatcg tcttccggaa tattttcttc
     8521 cacttttccc actaaatcgg ctcccacatc agcagcttta gtaaaaattc cacccccaac
     8581 acgtgcaaac aaagcctgtg tagaagctcc cattccaaaa gttaaagtag tagtagtgat
     8641 aataatcagt ttcatggaag aatcggaagc tatagtcaaa tttgtagcat tatccaatgc
     8701 accaataaca aactgcaaaa cataaaacca aatggaaata tccaataatc cgagcccaac
     8761 aacaaccaat cccataacgg ctccggaacg aaatgcaagt ctcagtccgg cattcaaact
     8821 ttgctgagca gcattagcag tacgggcaga agcataagta gctgttcgca ttcctataaa
     8881 tccggctaag cccgaaaaaa atcctcctgt cagaaaagca aatggcaccc atggattttg
     8941 tattttcaaa taataggcca taaaggaaaa aattatagtt attataagaa atacaatgcc
     9001 tactactttg tattgttgtt ttaaataggc cattgcacct tcccgcacat attttgctat
     9061 tttttgcata ctaatggtac cttcactttc ttttttcatc tgatgaaaaa aataccacgc
     9121 aaaaaataat gccaatacag cagaaacagg taccaaaaag aaaactttat tctccataac
     9181 gtattgattt taagtatatt aattaaaatt tcgaaattta ttcattttga taaaaaattc
     9241 aaatttaaaa ttattgagca cccgataact ttgttagcct ttgatataaa gtaggatgtg
     9301 aataatgaaa aaaagcatac aaaggatgag gcatcaaatt gctaagtgaa gtggcagcca
     9361 gcttttttaa accactctgc agctgctttg cccagccaaa atttgcagca aaagcatctg
     9421 cctgatattc gaattttctg gataaaatat tggtacaaat actcaacaac agcgaaacag
     9481 gtgtatataa aaggccaaaa gcaatgacgt tcaaatgaaa ggaagcagtt gttccaccca
     9541 aaacttgagc aaaaacatca cttttcagta tcaatcccaa taggaaaaat agtaaaagac
     9601 tttccggaag agaaatcaga aattgataaa gtgtgtgttt ctttttataa tgaccaattt
     9661 catgcgccaa aacagctact atttcttcag aagtaagctt gtcgagcaaa gtatcgtaaa
     9721 gcactactct ttttttgttt cccaaaccgg tgaaataagc atttgctttt gtcgaacgtt
     9781 ttgaactgtc gataatataa atattgtcca atttgaatcc ggctttggtt gcaaattttt
     9841 ctatctcatc gcgtaattct ccttcttcaa gcggcttttg cttgttgaaa ataggtacaa
     9901 tgatttgtga ataaaacatg gacatgaaca aactaaacaa ggtaacaacc gaccaagcaa
     9961 tcaaccagaa aaactttgga gttaattgat atatccaaac tattgcgccc aataaaatgc
    10021 ctcccaaaaa tacacttaaa gcaaattcct ttaaatgatc ctgaacaaag agctttggtg
    10081 ttaccttatt aaatccgaat ttctgttcta taacaaaagt atcgtaccac tgaaaaggaa
    10141 tactgatgag tgtttctgca acaaaaagta ctccgaaata caacaacggc aataaaatgg
    10201 gatgctcggt tattcggcgc agaagcgtat ccagccaacc aaatccgcct aaacagaaaa
    10261 aaattaatat tataataaag ttaatggtgg aagaaatggt tccgaaacgt atattggatc
    10321 gaaaatattc ttgctgttta ccgtatttgt cggcgtcata aatatctttt aaattatcgg
    10381 gaagttcatt tttggaatat tgaaaattca aaaaagacaa ataacgctct aaaataaaat
    10441 cacctactat aaagaataaa atcaaataaa acaaaatttc taccagtgac ataatctttc
    10501 gtttcaatta tttaataaat tgagtgcaaa tataattaat ttaatgattt cccaaccctt
    10561 tgggctcaaa atttacataa aaagcatatc tttgcctttc ctttataaaa atttcaattt
    10621 gtatgctgtc agtcatggaa atccttaaat atatgaagag attacaaata tttttattta
    10681 ttctcataac tcttttttcc ctttcctgtt ccaataaaac agcttccgat aaaatcagca
    10741 ttgccatccc ggaaggtcct tcagttgtca gttttataaa aatgatggac gattccgtgt
    10801 caattgaagg gaaaaaagtg gaatatattg tgaaatccga cccacagcaa attcaagccc
    10861 tcatgatgca aaacaaagtg gactttgcca ttttaccaac caatatggcg gccaatcttt
    10921 tcaacaaggg cattaaatat aaaatgatag cctgccccgt gtggggaact ttgtatattt
    10981 tgtcgaactt gcctgaagta aaaactttaa acgatttgca aaacagaaaa gtaagcattt
    11041 ttggtcaggg cactacagcc gatatcctgt ttcaaaaatt gatccagaaa aaaggaatag
    11101 aaaaagtttt tattgattat cgttttacgg gaaattttga actttcccaa gcgcttcgtt
    11161 taaagaaaac caatatagcc gttatttccg aacctttggt aagtaaactt ttggaacagg
    11221 attctaccat tcatatcgta gaaaaattgt catgcgaaga gtatctaaat gaaaaagata
    11281 gagatatatt cgtacaaact gcttttttag tcaatgaaaa attggaacgg aataatcctc
    11341 agattataaa ggaaatatgt aatgcttatc gtgccagttg caattatgtt actcaatatc
    11401 cggaggaagc tgccgtttta ctggttaaac atggcatttt accggaaaca aatgtggcca
    11461 aaaagtcaat tttgctgtgc agtatcaatt acgtagctgc ctttgccatc gaggatgaaa
    11521 tttacagtta tctgaaaatt ttttacgact tcaatcctga aagcattgga ggaaaattac
    11581 cttcagaaaa tttcattttt caagtttttt agccttaacg gatatgaaaa atcagaataa
    11641 aaaaattttt ccttttattc tttctgtgtt ttcgctggca attatttggc aaattatttc
    11701 tatatctatt ggttatcctg ctatttttcc ctccattttt gatttaatat cagatacgtt
    11761 acatttattt acccgctccg aattttattt tgcattattg ggaaccgtca gcagagggtt
    11821 attgggtttt gttatcactt tctttattgc tctctttttt ggaagtttaa gtgttttcaa
    11881 ttcatttttc aaggattttt ttcatcccat tgtggttttt acgcgctcca taccggtgat
    11941 atctatcgtg cttgtcgctc ttttgtggtt ttctccccct tttttacctg tttttgtagc
    12001 atttttcagc atgtttccca ttctgtttca aagtatccaa agcggattgg aaaatgtgga
    12061 taaaaaatgg gtcgaaatgg gcaaggtttt tggactttca tctcgaaagc gttttttaca
    12121 catttattat cccgctgcca gatcgactat ttttgacggc atcagttctg caatgggatt
    12181 tggatggaga gccgtaatta taggcgaagc actggctcaa cctgttcatg gcattggttc
    12241 gcaaatgaaa gctgctcagg cttatattaa tgtttccgaa attatagcat ggacacttgc
    12301 agccattttg ataagttata ttttcgagaa gataattctt tttctgaaaa agtgtaatcg
    12361 tcccattcaa ctacccaaat cagaagaatg taagttgcat aaattcaatc aaatagttga
    12421 taaaaaggaa attactattt ataacttaaa gaaaaaattt aatgattata ctgttttttc
    12481 gtctttcaat gaaaacttta aggacaccga aattaatttt atcaaagggc catcgggcat
    12541 tggaaaaaca actttattga ggttgatagc acgacttgaa aaacccgacg aaggaacgat
    12601 ttattattct tcaaaattca gttttggata ttcgtttcaa gatgttcgtt tattgccttg
    12661 gcttactgtt aaagaaaata tcgcatttcc actgttgtat aaaaaaacag atagacaaga
    12721 actcaccgat aacatcgatt ggctgctaaa taatatggag ttgacagaac atcaaaacaa
    12781 gtttcctgat caattgagtg gaggacaatt gcaacgcgtc ggattggctc gcgctttagt
    12841 tgcgcaaccc gacgttttgc ttttggacga accgtttaca ggttttgacg acgatttaaa
    12901 aacaagaatt caagatttct tatcgaaatg gtttgaagat tttcagccac tcgttatttg
    12961 ggcaacacat gaaaatattc tctttccgga aaaaaataga agagaaattc aacttaaaaa
    13021 ttgaaacgat ggatatctgc tgaaaacatc gaatttggaa attaaacaaa agcaacaatt
    13081 ttgttgggat agttgttaaa tttacgttga tttgactgat gtaaataatt gctacagctc
    13141 caataatcct tcgggcaaat ccatgtaaat aatttttttc atttcatcaa tttgtagaat
    13201 gtaatcttgg caagctggaa tcagtatctg cttttcgcca ttgtcaacta taaaaaggac
    13261 gttttgtgta ctggtatcga tatctgtaac gataccgatg aaattattgt ctttatcgaa
    13321 tattcgaaat tcgataaatg tattgttatc catatcaaca tcactttcca agatattcaa
    13381 aaatttttta tgcaaataaa tagtcaatcc taaaaaattt tttgcttttt tttcattgtt
    13441 aacatcttca atttttaaaa agccgctatt ttcagaattg aaacgccatt ctttcaaata
    13501 aaatggaaca aggatgccat gcatatcgaa aatcaaaaat gggatattat caaaatccaa
    13561 tccttcgtaa ttggacatga aagccatttc cccgttaatt ccgtgtaatt taattatttg
    13621 tcctatgggt ataacttctg taaaagaaat catctcagaa aaattttaaa aatccggaaa
    13681 aaaatttttt tcaacaaatc aagccatttt ccgaaaaatc gtcaaaatga aaaggctaca
    13741 cccgtcatga ggttgaatcc ttgaacatcg tagccataaa aatactgata tttgttgttc
    13801 agaagattat tcaagtgtaa atagactgaa agcgacttgc tgacaatata ggaaattccc
    13861 aagttaacgt ccattatgtt gtccattttc actgaacgat tgcctaattg agcatatctg
    13921 ccggttttgt aaaagaaata aagggatgtt gtgatatttg aagttattcc cagattggct
    13981 ctcaaatcag tttcccatgt cggctgtaac cagggcaatg ccgtgtcttt tgtatcccac
    14041 caattataag tggttcgaaa ttccatgtta aaacggttct tttcgtcatt gtatgccgcc
    14101 ctgcctccaa ttttaaaatg ataggcatcg ctgtaagcaa cattgaaccg attggtaaat
    14161 aaaaccgaat cgggagcatt caattcttcg gtaatgtatt ccttgtttac ataaaaatat
    14221 tgctgatcag taaatcgata atcggcataa aaatcgacga gtaaattttt agcaggtttt
    14281 attttgacac ctccatagct gagaaaaggg gtgtaagtgt cttttattcg tgtgtcggaa
    14341 aataggaagc aattttccga aaaaattctg tccatgctat tgatttcata atcgcccgaa
    14401 agaccaccat acaaagtcat ccactgagga aaaacattcc actcggcagc aatatcggct
    14461 gcaggactga aagaacgccc atgtttcatt gaaaaagcgg ctttcagtcc cattcgtaat
    14521 ttccaattgg tttgttctat ggaatagaag ggattaagca taataacgga ataataatcc
    14581 caaaagttga atagaggagc ttcattagcg gtatatttta tttgatgaag ttggacatcc
    14641 aagccaatat tgttttcgtg aaaaggcata ataacattgc ctgtaagatg tattcggttt
    14701 tctttgatac caaatttatt ttgaaagaaa tgataattca atttggcaga ataattgata
    14761 tcggaagtta aaggtaatga ctgcaaacct ccgaatgcat tgagatgcca gaaagtttcc
    14821 gtaccgggag tttcggacaa ttcttgtaag gtaaaagaat gaggcgcacg ggtgatggag
    14881 gtaaattgcc gttcaataaa ggtggatgaa ccatgatttt ttgccaattc atttagttga
    14941 acaaaggcgg aatcgttaaa attaaagcca taaaatttca atctttcatg ttgagtttct
    15001 actccggcaa acaaatgtaa gcttttcaat tgttggtcaa aaaccaatcc cgatttggtt
    15061 gttgaatgaa gtttgtcgcc gaaagttcca atatgattca tgtaaaagtc gagctttgta
    15121 ttgggacgat taagtaaaga atataaaaaa tttgcggaag aattatagaa gtttcccaat
    15181 cccaactgta aaaaattgtt ttgttctacc ctgttattaa aaaaaaactc agttgacgac
    15241 aacggatgaa tattgttatt aatggaaagg ggaataaaaa gctcagtata ttgagccggt
    15301 agaggtttta cttcgggttc aataagtttg ggtgtaacat ttaattttcc ggtatttttt
    15361 agtataggtt gatattccct ttcaactgta atgtttcggg tgaaaacaga atcctgagcc
    15421 aaagatatgg caaaatttac acagctgaat atagataaaa atactaattt tttcttcaag
    15481 tttatcatat caatttacgg tttgtgaaaa taaatacaat tttgtctaaa aaaaacattt
    15541 ttgtcaatca aagagttatt cctaaaatgt caatgaataa tatttttctt ttctctttgg
    15601 ctaatagctg acaatcgctc atcaatcaaa ccctgaattt catcctgaat ggtataattt
    15661 ctttttaagc ttaaaaggta ttgttttgct tgaaaatcat tccctttttt tatgtatata
    15721 tctgccagca gtacaaaact acgagccaac caaaattgat gtggcgtatt tttttttgca
    15781 aaatccaaaa tttcattttc tgcttcagtc agtttttctt gttgaaaata taccgaagct
    15841 aacaagtatt ttgattctgc tccaaaagct gttttggtat cagcagaaat aattttcagg
    15901 tcttcaatgg ctgcatttag ctgttgatga agaagatatg ctttagcacg gtaaaacctt
    15961 gcttcggtct ttacaatagc ttccgaatat ggatcttgca aaattacatt ggcaatatcg
    16021 ataagacttt gataatcatt agtaaaataa ctacaacgta acattcccaa atgagcagcc
    16081 tgtttgttag ccgaattctg agctacttgt ttaagttgtt tgaagtaatt gaaggcagat
    16141 gcatagtctt tctggtcata ggtaatttct gcacaatgca gggcagcttg ttcaacatat
    16201 tcggcagcat tggttttcag tacaacttca taggctttca gtgccttatc tctttgctgt
    16261 gtgcgataat agctgtcagc cagatagaat tgagcaatgg tacaatacct gcctccggga
    16321 caaaaattat tcaaataatt ttcaaaacct gagatagcaa cagtatatct accatttatg
    16381 tattgttttt cggctgcaat gtaagaaatg gaatcttcgt gcataaccga taagggacga
    16441 acaatacgcc ccgaattttt ggtatattgc aaaaaaccat tcacatcgtt taattctata
    16501 tacgcacttt ccatgctttt aagagcaata tatgattctt cggtgtcggg atatttttcc
    16561 gctacatact tgaaaataac aatcgcattt tcaaaatctt gtcggttgaa ataaatcata
    16621 ccactttcaa gtgctgcttt tctggataat acagatgaag gatactgatt cattaatcgc
    16681 tcataagcag acaaagccat tttttcgttt ttaagcatca aataagaacg accaatctca
    16741 tagagggcat catcgccata ctctgaattg ggatatttca acaacaattg ctcgagacga
    16801 taaattttgt tcaaataatt tttttgtaat ccttctacgt atgctgcctg aaaaatagaa
    16861 taatccgctg tattgggaga tgtaaaagca gctttttcat aaaaggaaag tgcattttta
    16921 aaatttctgg cataaaaatg acaatctcct gctcgagtta acgcatcagc atagagcatt
    16981 gatttctctg aattttcatt ttcaagatat ttggaaaacc attccaatgc cttgatataa
    17041 tttttctgat caaaatatcc ataacccaaa gaataatagg catataataa attttcgctg
    17101 tttctggaca agggattttt aatgaatgtt tccaaatcta caattgcttg atcgggattg
    17161 ttggtgcgat agtagcattc ggctctccag aataagcatt cggcagaata ttttcctttg
    17221 ggtgcagatg tatgggcaag agaaaaatat tcgatagctt ttccataatc cttctgagca
    17281 aaggcattga cgcccatctg atagagaaga tattgtttgg tctcggttaa agatgtattt
    17341 aattttctga ttttcaaaat agattgataa gccagttcat aattttttgt agttagataa
    17401 acggaagtca aatattgata agcctcgtca gcatatttgg agtttggaaa ctcggaaaga
    17461 aaacgggtaa aagctgcaac cgattccccg aatgctgaag ttgtttcata acaagttaaa
    17521 gcataattaa ataaagcttc ttctcgaaca gttggattaa agtgtgtttg caaagcggct
    17581 tcaaaagaaa ggcgggcatt ggttttatcg ttcaagaaaa catatgatgt tcccagatga
    17641 agataggcat tttcggaaat ttcatcaggt tcggtggtaa cttttgatag atattggatg
    17701 gcattggaat aatctttcag ttgaaaatag gacaatccca ataaataaac atcgtttctg
    17761 agaactttag gggacagatt ttcgtacttt ttgaaataag atatggcttg tgcataattt
    17821 tgttcgccat aagccaattc tccaagtatt cggaaaattt ctgcattgtt tttattattg
    17881 ggatattttt gaagtagaaa gtcagctttt tctttcaatt ttgcgtattg tttttcaaca
    17941 taataaattt gtaccaaaaa ataaggaaca ctaatttgat agtcaggatg attttctata
    18001 gctaaaaaat cggggagggc tttttgataa tttcccatca tatattccga ataggcatag
    18061 taatatgttg catccacacg aaagggagaa ttctgaagtt ttaatcgaga aaagattttt
    18121 ccggcttctt cgtaatgttg tgtttgaatt gcggtatagc ctttgtaaaa aaggaattcg
    18181 ggaattttat tttcagacag ctcctgttcg tttacttgag aaaaattttc tctggcaact
    18241 gtgtagtttt tctgttcgca gttaagtacc ccaatcataa aataaatttc ctcagaaaaa
    18301 ggtgtataag gatgtttggt gagaaaagtc tgtaaaagtg aaagagcgtt ttcttttcta
    18361 agttcgtatg cattggccga caggaaatat tcggcttcct gaaactgtcc atcagaggga
    18421 aatgtttttt tttctaaaaa ctgttcaaaa cttcggtaag aggcagcgta tttttgttga
    18481 aaataaagtt ctttcccttg aatgaaatct ttatcttcgg aggcaaaaaa taaggtgtgt
    18541 tgtgcggaaa gaaataagtt gcttaaacaa agaaaaagaa taagagttaa aacaggaata
    18601 tttttttttg ccatttttat agaatttatt ttttacaaac aaaagagcat aattaagcag
    18661 gacaaaatgt ggaaatttat tgcaaaatta aatgttttga cactttttgc caatttgttc
    18721 ccgtagaaaa atttcaactg cttttttaac cgatttcaaa ccaaaaagtt caagttgaat
    18781 tttttcaaga ggcacataaa agcagtcttc cacatcatcc gaaggtatgg gtatcaggtc
    18841 tttttttatt tgacagagaa aaaacaaatc gagcgtagga ataagcaaat tgctgtattc
    18901 gtattcgtta ggtaaggaga aaaggtaagt tggattttca acttcaagct gtaattcttc
    18961 catgatttcc cttttcaatg cttcttctgc tgtttcataa ttgtctacaa agcctccggg
    19021 taaatccagt gttcctttgg cgggatcttt ttttcgacga caaacgagca actctttttt
    19081 ttcgtttaaa ataaaagcag ccactgctgc tgatgcattc aggtaataaa caaaaccaca
    19141 agccttacaa cgttttgatt tttcgttgtt tatctcaaaa tctgcagaac cacaggaagg
    19201 acaaaaagaa aatttcgaaa aaaaatccat tacttgttct ttcccttgat atgttttttt
    19261 catttaaaga atatctttaa aattaaagat tacagttgaa aattgtaggc atgtcgtaca
    19321 ataaacatgc catttgataa tttataaaac gttgaccata gtatttctgg tatttgtaat
    19381 tgaaaaagtt gtttataact ttgtcttaac tcgtttattt attgaattta aaatgttatt
    19441 tttgcaaaaa aattaatacg tatatgggca aaaagttatt gcttcccgga gatgatgagt
    19501 tcaaagacct tgttcacagg tatgagcagt acctttcggg caatgcaaat ggatacttcg
    19561 atgtggaaga atttgaaaat attgcggaat actatttgct gagaggaaga accaaagatt
    19621 cttccagagc tattgatttt ggcatgaaat tacacccggg cagtacagca ctcaaagcta
    19681 aacgggcaaa aatatatttg gctgttgatg aagtaaaaaa agcttatcaa ttgctttcat
    19741 cttttaccga aagcgattac gaaaccttat tgttgaaaat tgaagccctt gttcgcatgg
    19801 gtcgtcatgc tgaagctgat aaattgataa aaggcttgac aacagaaaaa acagccgatt
    19861 tggataatat ctttctcgat atagcttata tttatctcga gaacaataaa tttgagaaag
    19921 cttcggaatt tttattgaaa ggctttcaaa ttaatccaaa gaatatcgat ttactttttg
    19981 aattagcttt tagttttgaa cagatggaag aaaaagaaaa agctattgaa atttataacc
    20041 gtattcttga tttggatgct ttttccggag aagcttggtt taatatagga caaatttatt
    20101 tcaatttgca ggaatttcct aaggctttag aagcttatga ttttgtgctg gctattgatc
    20161 ccaatgacag tctggcccat ctgcaaaagg cacatgttca ttatcagctc gaagagtttt
    20221 atactgccat tgaatattat gcccattatg ctgccatgac cgatgaccca gcttcgacta
    20281 gcctctatat tgccgaatgt tatgaaaaac ttgagcagta cgacaaagct gtagattatt
    20341 ataaatattt acttgaacaa gatccggata acaccgacct gcttatttcc attggatctt
    20401 gtttattgga acaagaaaaa ttctcagcaa gcattgaata tctttcacgt gccatagaac
    20461 ttaatgattc attggccgat gcttgggttt atctggctga agcttattta gccatcgata
    20521 atctggatat tgcattaaag tcttatttgc ttgcacttca actcgatagt gagcagcctg
    20581 aagttataat ggctgtagca aatatttatt tggaaaaggg agattatgag actgcattaa
    20641 atttttatct ggctgttgag gcaatgagca attcattgga aattgtcaat gtttttattg
    20701 ctgccactta ttataaattg gggaataaga ccgaagctta tcgttacttg aaaaaaggaa
    20761 tagaggataa tgaagatgtg ctaaatctgt ttttggaaat ttgtcccgaa gctatcgaga
    20821 cgatccatta attttctatg gatgcagtta ctcaaaaaac tttttaccgc gtaatgtaat
    20881 ttatttatgc cggtggctac cttatactta agaaataaat tttgatgaaa atgagactgt
    20941 atgggttgat tggttatccg ctaaaacact ctttttcaaa acgttatttt gcagaaaaat
    21001 ttaggaggga aggaattgat gctcgatacg atttatatga acttcaacgc atagatgatt
    21061 tccctgaact ggtcaaaatg cctggccttt gcggcttgaa tgtaaccatt ccatataagg
    21121 aaagcgttat tccttttcta aatgaattgg acgaagtggc tgaaaagata agggcagtaa
    21181 atgtcattaa gtttttaagg aaaaacaatc atttgtggct caaaggctat aattcggata
    21241 ttattggttt tgaaaaggcc ataatccctt ttttgaaaac ttttcatgac aaagcattgc
    21301 tactcggaac cggaggagca gccaaagcga tttcttttgt tcttgcagct cataatatta
    21361 aagttactca tgtttcacga aagccaggca tcaataaaat aacctacaat gatttaaacg
    21421 aagagttgat gcgtgagcat aaattaatta ttaatgccac accacttgga acctatccta
    21481 acctttgctc atgccccgac atcccttatg aacatcttac gccacaacat ttattgtttg
    21541 atgttgttta caacccacct aaaactttat tcttgaaaaa aggggaagaa caaggagcaa
    21601 ccataatcaa tggcgaaacc atgttgagtg gacaagcaga agctgcatgg gaaatatgga
    21661 acaaatagaa gatagtatag tttatttttc agagcaagag aggaaggact ctttcccctt
    21721 ttgctccgat ggaaaaaaat gactataatt gttcaccaaa cgttttgata ggcaatcatt
    21781 aaaattatgc tgattttttt gattatactt tccttattta ttgggaaaat atttattgaa
    21841 aaacaatatc atatcctgcc atgaagcctt atcggctgct tcgttatatg caatgggcat
    21901 attgaatttt ttccctgctt cagtagaata tttattggta aaagcatgag tagcaccttc
    21961 ataatttttg aattcgtaag ttatattgta tttttgcatc tcacttttaa aattatctat
    22021 atctgcttgt gtaacaaaca tatctgctgc tccattacaa ataagcactt ttgtattttt
    22081 catcgccgga ctggctttaa agccattgag cccaccatga aaacttacaa ccgtttccaa
    22141 aggcagtccc attgcagcag catttagcac cactgtgcct ccaaaacaat agccaattgc
    22201 actaacatgt tttttatcaa cagttttttc tgaagtcaga atttcataac ctttttccat
    22261 ccgctcttta agtaattctg gatgagaaaa aatatatccc gctgcgtttt gagcttctgc
    22321 agcagtatca tataccttac cgtctccgta catatcaact gccatagcca catagcctaa
    22381 ctctgcaagc atgtctgtac gcatttttat gtattcgtta aatccccacc actcatgcac
    22441 aaccagaatg ccgggttttg ttttttttcc atcatctttc catgcaagat atcctctaaa
    22501 attttgatcg ccaattttat acgaaatact ttttgtttga atatctgatt ttttgtttga
    22561 gttgttgttt tgagctgttc caaaaacagg taataacagc atgatgccaa acataattga
    22621 tataattgtc tttttcattg ttttaaaatt aatttattat ttatactttg attgattgat
    22681 ttgatttttg aaaatatata aaatctatta atgagaatag aaattgttgt taaatttcac
    22741 atccatcggg gccgcaaact ccgccatttt caccggaaaa ctcaataata tcggtttttt
    22801 tgtgttcagc taaagccctg ttgagcgctt gtaaaaaaac cgattcatcc tgtgctcccg
    22861 acacggcaaa tttatcgtcg aacagaaaat atggcacacc tctgacgtag agctgatgcg
    22921 catgggcaat atcgtgtttg acttcatcgc tataagcatc cgaaagtaat acttcgtttg
    22981 tttccgtagc ttccaaaccc acttcctttg ccaattgaag taaaatttca ttgtcatcga
    23041 tatttttccc atcagtaaaa taagcagcaa aaagtttttc ttccatttcg ttttgcttgc
    23101 cgtgtttttt ggccagatgc aataatcgat gagccttgaa ggaatttact acaattgcct
    23161 tttcaaaatg aaattccaat ccgacttcct tagccatctg tgtaatgaag gcagtggatt
    23221 ggagcgcttg ctccattgac caaccttttt cggcagccaa atgttccatt gttttcaact
    23281 caggattggt aacggcaaaa ggatcgagtt gaaaactttt ccattctact tgaacatttt
    23341 ccctgtaagg gaatttttca agcgcttttt caaatttatg tttgccaata tagcagaaag
    23401 ggcaaactac atcactccag atttcaactt taatcacaaa attattttta ttttccattt
    23461 tttattgtta aattattgtc agttttagcg gataagtccg ggaacttttt cgccatgaat
    23521 aaattttaat attttttgta ccgcatgttc agtcccaatc agcaaggcat tgtgaaccat
    23581 agccgaattg tggggtgaac ccaacaagtt gggtaattca aaaaacggat aatgaagttc
    23641 aaattttcga tccttaaatg gttctaccca ccaggcatcg atccccgcat aaaaattcgg
    23701 atgtgttttc agatgttcat acaacgcagc ttcctcaaca attgcaccgc gtgccacatt
    23761 caccagcacg gcatcttctt tcatcaattc caatctttct ttgttgatca aaccttctgt
    23821 ctcttcattc agcgctatgg aaaggatgag actatcagca ttttgcagaa catagtccaa
    23881 atctttcaag gttccaataa aatcgacctc ctcatccgtt ttaccggaac tgttggtcgc
    23941 catcaacttc acaccaaaag gtcgcaataa tctggctgta gcttttccga tagctccaaa
    24001 accaattatt ccggcaaccg aacctttcag aaatttggtt tgacttttta tctgactgaa
    24061 attgcccttt gccaagtcgt tgtgataaac atacagcctt ttgctgagcg ccaacaacat
    24121 agccatcaca tgttcggcca ttggttcggc ataagctccc tgattggcag ctatctgaag
    24181 tttttcagga aaatcttcca accgaatatg atcatatccg gcagaaagta attgcataaa
    24241 cctgatatta tccataggca aattttgttt gttaatgcct tcctgcaccg gattccacga
    24301 caaaaggaca tcggctttag tcatcagatt ttccaattct tcttttgtgc aatcttcctt
    24361 aaagtaaatt tgcatttcct ttcccaatac attctttaaa tactcttttt gttccggaaa
    24421 agttttaaaa gttactatta tagtcatttt cttttttatc attttatttg attgatccac
    24481 ttttcgagtg tttcataagg ctgtgcaccg gttaacttgt atttgccgtt ggcaatcagt
    24541 gtgggtaccg aattgatgcc atactgataa gcaagataaa agtcctcatt aagcatttgt
    24601 tttacttttt cggtgtgaaa atctttctcc cattgttcga cattcaatcc cacctcacgc
    24661 gcacacattt ttagcacttc aacatcatta atattaagac attcagtcaa atgagctttc
    24721 tgaatacgat taaatatttt gtgatgcatt tcatttccac cctgcaattc ggcagcctta
    24781 caagccagca atccccgagt agaatacgga tagtcaaaag ttcgggttgc cattaattcg
    24841 ggatgcatgc gatgagcatc atcattcatg ttggcagctc tccaatgccc taaaatttgt
    24901 atttttcctt cctccttcga accgaaaatt ttttctatcc catttggttc gggagccagt
    24961 gcaaatgatc gatgaataac ctcaatgcga tcaaaatatt tctctactaa tttttccact
    25021 ctgggcgaaa gtgcataaca ccatgcgcaa agtacatcgt ggaaaaattc tatggtaatt
    25081 ttattttgtt ccataatttt tttaaccaaa acgatacgac gaaattttaa ctttcaaaag
    25141 tgttcccgaa aaatcctgtt tgcctaagtc gtttccggca gctccggcaa ccacaaaaag
    25201 cggaaccaaa tgttcgcttc gcggatgcga ttctcttgca ccgggcgcac ttaaccactg
    25261 agtaagaagt tgattacgaa tttccggatt tttattttct actgcatgag tcaaccattt
    25321 gtcaaactgt tccgaaattt tcgaaatagc ctgattgccc gacatcattc cacgcaaatt
    25381 atgaaaactc attccggaac caataataag taccccttcc gagcgcaaag attcaagtgc
    25441 ttttcccata gcaatatgct gagccggtcg aagtccctta atgagcgaaa gttgtaccac
    25501 aggaatatca gcttgaggaa aagctaccat taacggtaca aaaacgccgt gatcaaatcc
    25561 gcgaagattt tcacgagctg ttgtaaaacc ggcttcgagc aacaggtttt caatctttaa
    25621 cgccagttga ggatcgccgg gagcaggcca gctcaattga taagtaaatt cggggaaacc
    25681 gtaataatcg tacagcaatg acggatgctc accaaaattg acggttggca ccgattcttc
    25741 ccaatgacct gagacgacaa gaatggcttt aattttatca tgaaaatcat tgccaatttt
    25801 ttttaaataa ttacgcagca ttccatatcc ctgagggtcg cccatgaagt catccatcac
    25861 atgccatggc ccacctccat gaggaataaa gaataccggt aatttttctg aaattttcat
    25921 gttgttgaat caatctgaaa atagacagat tgtgtccatt ctcaatttta cttttctctg
    25981 actgttttta aagcagttcc ccaagctatg agctgatcca gcatggcatg caagctgcct
    26041 tcgtgtgaag aagcaggttt aaaaacactg aaattttcaa aatccgtaaa gagtgaaagt
    26101 gttacctgag ctctaacgtc ggctatctga atttcaccca tgatttggcg aagctgttct
    26161 acagctcttg caccgccggc agagccataa cttacaaatc ctgctgcttt gttgttccat
    26221 tcggcataaa caaaatcgat agcatttttt aaagctgctg tcggtgcatg attatattct
    26281 ggtgttacaa aaacatacgc atccatttcg ttgattttct ctgcccattt ttttgtatgt
    26341 tccttttgat actgatgcat ggaagccgga atcatttcat cgagcaacgg caaattataa
    26401 tccgcaatat caactatttc aaaaattgca tcattacgtt tactggctac ctcataaacc
    26461 cattttgcaa cagcttccga gttacggccc gggcgcgtac ttccaactat tattcctatt
    26521 tttatcataa tcttttcatt ttttactgtt tattttttct tccaattcat ccagcctatc
    26581 gttggccatg tgcaccatca tggttacccg tacaaaaggc aaagccaaca agagcaaagc
    26641 caacgagata atcagttgaa accagttgaa ggcaacgata tttacaataa ttccgataag
    26701 tccaattaaa gcggcaataa ttccacccgg tattccaagt ggacatctga gttttttctt
    26761 tgtttccatt tttttgattt ttaattactg ataatttttt aatttttcta tcaactcttc
    26821 tttcgtcggc acgcttttga acaccacctt gttgtcaatc aacaaagtcg gaacggtatg
    26881 gatacccaat ttcatggttc gtatcattcc gcttggtgaa ttggcgaggg tggttttcag
    26941 tgaaaattta tcctcaaact gaggtaaaac ttctttcagc atttgcttcg taagtttaca
    27001 gttcggacaa gtcaacgtat aaaagagttc gatagttgtt tttttattca ttttaaagga
    27061 tttataaaat ttctttatgc ttaaaaagta taaatattac tcacgacaaa aataaaattg
    27121 aaaaaaatat tcttgagact gtaatttttt tgtaaaattt gccgttcctt gtacttttcg
    27181 taagtctgtt gtctgcaaaa tattttttta taaataaatt tatttcaaat ttgtataaat
    27241 aacatgcatt aaggaaaaaa gttcatgttt ttttgataaa gcatttgcca tttacaaaat
    27301 aaaattgctg ttttttaaag caaattattg taacgtattg taacaataaa aaatttaacg
    27361 actttcttca gcttaaattt tgcttttgct ttgtttccat attcaaagtt taaggagaaa
    27421 ggtaaaaaat gtatttttat tcgttagttc aaaaattcaa aaatatcccg taattttgca
    27481 ccgcaaaaaa gaaattaact gcaattgcct tcttacctcg ttttttcttc ggaagtataa
    27541 ggtttaaaaa cagctcaata ttattaatgg atttggttgg attttttatt cttggaatag
    27601 cggcaggaat tttgtcggga ttgtttggca ttggaggagg aatcgttatc gttccctcgt
    27661 tgatagtgat ttttggtttg gacattttgg atgccaatgc tgtttcgctg gcagccatgt
    27721 tgttgccggt tggaatttta ggcgtatggg cttattacaa agaaggttat gtaaatattg
    27781 gcgagtcgct ttgggtagca ttggggttgt tcttggggtc atttgttgga ggtggaattg
    27841 ccgtaaatat tggcgaaagt ttgcttgcca agttatatgc cggaatttta atctatattg
    27901 ccatcagtta ttttcaatta ccaaagtatt ttcggaaaaa gcagccgata gaaaaagaag
    27961 aaaaaccacg cgaaaaaaaa tcattctggg catttgttct gataggtttg gcagccggag
    28021 tttttgccgg attatttggc aaaggcggcg ggctggttat tgttccgatg ctgattcatt
    28081 tttttcatta cgacagtcgt tctgctgctg caacttcatt ggctgccttg caacttccgg
    28141 tcggcttgcc aagcgtactg atttatgctg aaaacggaca tttgaatttg ctgtttgccg
    28201 cactgatcgc tttgggtatt tttgtaggag ttttaatagg ttcaaaaatc ggcattaaaa
    28261 ttccaccatc aattttcaaa aaaatctatg cggtttttct gctggcaatt gctgtttata
    28321 tggcatttga atatatttga aacaattgat ttcaccgaat cgaaatactt taataaactt
    28381 tatgattatc cgcaatgatt cctattgtaa attcttgact tgaaatcagt gttgtagctc
    28441 acagcagcaa caagcaacct gtcgaagatt gcatcaccaa aataa
//
LOCUS       DAOSCH010000012        28167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1236_length_28167_cov_6.372190, whole genome shotgun sequence.
ACCESSION   DAOSCH010000012 DAOSCH010000000
VERSION     DAOSCH010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 28167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28167
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1236_length_28167_cov_6.372190"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /locus_tag="PLZ54_02130"
     CDS             <1..1180
                     /locus_tag="PLZ54_02130"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOJ65771.1"
                     /translation="LPAKEHQNAISAYNFYRLIHHAQKVYFLYNSSTEGFQTGEVSRF
                     LYQLEYLYDTKVERKNITYDIFLKDAVSASVEKDSLIREKLQRFVSSGDDKLYLSASS
                     INAYIDCPLKFYYAQIAGIKETEEITETVENDLFGTLFHSSAELLYKPFKDVQLGKED
                     FEKILRQPSLVDEAISLAFSKHFFKNEQNGKIPPLEGNNLLISKILKKFLMRLLYVDQ
                     QLTPFRYLGAEEFCSIRFPLETLNLRVNIKGYIDRIDETENFLRIIDYKTGTGNTTFK
                     LLDDLFDGSLPMKNRPKHIFQVFMYCLLYRSKAEKKIISPAIYYLKEIFSSGFSSSIQ
                     FKPEKHTIQVEDFGIFEHEFKEKLTLLLNEIFNPEIPFKQTQIKENCDNCSFMEICNR
                     "
     gene            1358..1830
                     /locus_tag="PLZ54_02135"
                     /pseudo
     CDS             1358..1830
                     /locus_tag="PLZ54_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613208.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
     gene            1962..2729
                     /locus_tag="PLZ54_02140"
     CDS             1962..2729
                     /locus_tag="PLZ54_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOJ65772.1"
                     /translation="MKKFFSLLSLFLFVSILSQAQNELFLKKLFIYKGDTLPYRILYP
                     ENYNKSASYPLVLFLHGAGERGNDNEKQLVHGASLFTDSQNRQKYPAIVVFPQCPENG
                     FWAPIKERDNGFSFINSKEPTPSMQMVIRLLKQIRKEEAVDSRRIYVMGLSMGGMGTF
                     DLICRQPKTFAAAIPICGGVSLERLKKVKHMPIRIYHGGSDDVVSPEHSRNAYIELKA
                     LGSTKVEYIEFPGVKHNSWDFAFAQPDFLSWLFSQKK"
     gene            2863..3090
                     /locus_tag="PLZ54_02145"
     CDS             2863..3090
                     /locus_tag="PLZ54_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOJ65773.1"
                     /translation="MAKIKGTVVVDTERCKGCNLCVVACPPKALSLSKEANSRGYNYS
                     FLSNPDACVGCVACAYVCPDACITVYKMRVQ"
     gene            3138..4244
                     /locus_tag="PLZ54_02150"
     CDS             3138..4244
                     /locus_tag="PLZ54_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOJ65774.1"
                     /translation="MNKNSKTDNKEEVLLMKGNEAIAHAAIRCGCDGYFGYPITPQSE
                     VMETLMELQPWTTTGMVVLQAESEVASINMVYGAAGCGKRAMTSSSSPGVSLMQEGIS
                     YMVGAELPCVIVNVMRGGPGLGTIQPSQGDYFQAVKGGGHGDYKLLTLAPASVQEMAD
                     FMPLSFDLAFKYRHPVMILADGIIGQMMEKVVLPPFQPRRTDDEIREECPWATLGKTS
                     NRPQNVISSLALEAEVMEAINLRIQKRYREMEKNEVRYETYMADDADYLIVAFGTCAR
                     VSQKAVEMARENGIKAGLLRPITLFPFPKDIINQLASSVKGMISIEMNAGQMVEDVRL
                     AVNGKVPVEFFGRLGGIVPSPDEVYDALVSKLIK"
     gene            4319..5080
                     /locus_tag="PLZ54_02155"
     CDS             4319..5080
                     /locus_tag="PLZ54_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ65775.1"
                     /translation="MSNNEIISPENLVYAKPKSLKETYMHYCPGCSHGVVHRIIAEII
                     DEMDIQDKTIGIAPVGCAVFAYNYIDIDWQEAAHGRAPALATAIKRLLPDRYVFTYQG
                     DGDLAAIGTAETIHACNRGENIVIIFINNGIYGMTGGQMAPTTLPDMVTSTTPYGRKT
                     ELNGYPLNITNLLAQLPGTCYVTRQSVQSVAGVRKAKKAIQKAFENSMLNKGTSIVEV
                     VSTCNAGWKMTPENANKWMEENMFPAYPLGDLKDI"
     gene            5217..5762
                     /locus_tag="PLZ54_02160"
     CDS             5217..5762
                     /locus_tag="PLZ54_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOJ65776.1"
                     /translation="MKEEIIISGFGGQGVLSMGKILAYSGLMQGQEVSWMPSYGPEQR
                     GGTANVTVILSDEKISSPVLSSYDTAIILNQPSLERFESKVKPGGTLIFDGNGFHKFP
                     TRTDINVYRIDATEYATAHNAAKTLNMIILGGFLKVRPIVKIENVLEGLKKSLPERHH
                     HLIPQNEKALQIGMELIKKVN"
     gene            6024..7277
                     /locus_tag="PLZ54_02165"
     CDS             6024..7277
                     /locus_tag="PLZ54_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOJ65777.1"
                     /translation="MNNFTAVLITLFFSAFFSGMEIAFVSSNKLRFELDKKQRSITSS
                     ILTIFYKHPQQYISTMLVGNNISLVIYGLLMAKILSPLLQILGSQFLITFTQTIVATA
                     LVLVTGEFLPKSIFRVNPNFWLSIFSPLLLFFYVILYPITLLATSLSFGIMRLMGIKI
                     NPLANENAFSRVDLNYLVHESFENTEHKQDVDNEVKIFQNALDFSKVKLRDCLVSRTE
                     IVALDYNVDLETLKQTFIETGFSKIPIYKENIDNIIGYIHSSEMFQHQNDWREHINQI
                     PIVPENMAAQKLMKIFLKEKKSIAVVVDEFGGTAGIVTLEDIMEEIFGEIEDEHDTHD
                     FVAEKVGENEYVFSGRLEVKEINEKFHLNLRESDAYDTIAGFILYHHQHFPKLNESIQ
                     IDNYTFKCIKITNNRIELVKMKLQK"
     gene            7401..9515
                     /locus_tag="PLZ54_02170"
     CDS             7401..9515
                     /locus_tag="PLZ54_02170"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOJ65778.1"
                     /translation="MATLEKIRSKGVLLIVVIGLALLAFIVGDFLNSSSTFFNQSKEK
                     IAVIGDENISIHEFSAAIDQMTEVYKIETGQTNLNEEVVSQLRASVWENMLMEKILTH
                     EAKKIGLSVSPEELSERLIGKNIHPIILQRGAFMDETGQFSRSLLVQFLNSLDVTPTN
                     NEMKQQIDQAKNYWLFWEKNVKNAILQEKYNKLITSAISANSIDAKMNFEARKITVDA
                     AYVMQPYYSVPDSIFKVSSKELRDLYNKRKELFKQEKSASINYVALEIKPSQEDFQEA
                     EEWINKLEDEFRTTDDVVGLVNTNSDIMYDGRYYSEATVPAMLKDFAFRGKKNDVFGP
                     VFENDTYTMARIMETDILRPDSVKLRHIFLTKENEGKADSIVKALRAGADFATLARKY
                     SAVPQTAANGGEIGWLPDGAQGVDKQILENAFKKPLNEVFVIKDIQGTQIMQVTDRTP
                     LRRKVKLAILQRKVTPSSKTYSQIYNQAKQFAAELDAEKFVSRAQEKGYIVQKTENLN
                     ENTEQINNLPQSRQIVRWAFKGGKGDVSDVFDCGNYYVVAMITEKNEKGYMPIEKVSA
                     QLKSEIIRDKKAAQMINNISEQLKKTPDLEALAASLNAEIKMAENVNFTSYQFGLAGF
                     EPYIIGKATIVPLEKVSGPFQGNAGVYVMKTSNKKENTETFNAQQEIIQLNARMLYSL
                     PYLILENLRERTKIEDYRSNFY"
     gene            9735..10760
                     /gene="rlmN"
                     /locus_tag="PLZ54_02175"
     CDS             9735..10760
                     /gene="rlmN"
                     /locus_tag="PLZ54_02175"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOJ65779.1"
                     /translation="MPIPLLGASLEELTDIVSQLKMPTYTAKQISDWLYKKRVADINE
                     MSNISVKNRDLLKEKYKVGRLAPVKVTVSKDETKKYLFRTEKDHFIETVFIPEEHRNT
                     LCVSSQIGCKMNCVFCMTGKQGFMGQLSASEILNQILSVPEAEKITNLVFMGMGEPFD
                     NTVELLRTLNILTANYGYAWSPYRITVSTIGLIPGMQTFLEKSNCNLAISLHSPFHSE
                     RLELMPIEKAYPISQVVEVLKNYNFGRQRKLSFEYIMFDKFNDTMRHASELVRLLKGM
                     NARVNLIRFHAIPNVSLRSSSDERMKWFCDYLNNNGILATIRKSRGEDISAACGLLST
                     LENKKMS"
     gene            10773..13862
                     /locus_tag="PLZ54_02180"
     CDS             10773..13862
                     /locus_tag="PLZ54_02180"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ65780.1"
                     /translation="MRKIINKNKFHLISEKTILLFIIVLAGLLPKPVFAQSLLEKQIS
                     DSLTSIANKYTFVEKISVDNIYINNREKKIIIKANEKFGYMPFREENVKHIYRVLKQI
                     FSPSYKNYKIVCMVDGQTIEELIPNFYRTKRKRDETRIFDPKKPKYSLVKNLSKPYNV
                     TNGLERKNIALWASHGLYYEQSISRWEWQRARVMQTVEDLFTLSIVYPYLIPMLENAG
                     ATVLTPRERDMQSNEVIVDNNNKDSLSYREFNLQEVWTESQDFGFANFKSFYLQGENP
                     FNMGSYRQIHSVEKENKASFVEYIPDFPKAGKYGIYVSYKTLPQSTENAHYTVYHKGG
                     KTKFSVNQTMGGGTWIYLGQFNFDKGRNENGKVVLSNFTGEKEEIVTADAVKFGGGMG
                     NIARSPDQNGILENHKSSEIKNPALKKEKVLSWETEPETSGYPRFAEGARYWLQWAGF
                     PDSIYSPNRGANDYIDDFQSRAKWVNFLTAGSSLHPKGKGLNIPLDLSLALHTDAGTT
                     KNDSVIGTLGIFTVGKGNGKSYNNKVSRWASRDLTDIIQTQIVEDIRMSFNDSWIRRG
                     LWNKSYYEARVPEIPTMLLELLSHQNYADMKLGLDPRFQFLVSRSIYKGILKYLSALN
                     DEEDYMVQPLPIRQFSCIFVDKNKVKLRWKTRMDSLEPSAVAEHFVVYTRIDDGDFDN
                     GVLVSDTEYLKVLEQGKIYSFKVTSVNRGGESFPSEILSVCSHPHEKGNVLIINGFER
                     VAPPAGFVIDTTYAGFLNDMDAGVPYLYGYNFTGKQYEFRRDRKWVDDDAPGFGASHA
                     NYETQILAGNSFDYPFLHGKAICAAGYSFVSCSVEAVMNDDINLNDFQTVDLILGKQK
                     QTDVGTRENISLFKTFPLALQQKINLFCHQQGGNLLVTGASIASDMFDRNDALPDDIR
                     FLENTLKIKYRVHQAAITGNVKFVSSPFKQFVRHQFSYYSEPNQYSYFVESPDAIEPA
                     ARDAYTFCRYAENNLSAGVIYSGKYKICALGFPFETIVSDAEREAFMKSVLDFFAKP"
     gene            13949..15067
                     /locus_tag="PLZ54_02185"
     CDS             13949..15067
                     /locus_tag="PLZ54_02185"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOJ65781.1"
                     /translation="MKRAFLRFHARIVERIRVSNISTLKFNFILTKTKTTFVLTLLLI
                     MSGCNSNKSSLPGITGSMYEVLVVMDHQQWISAPGRTLVDLLDQDMEALPQPEPVMDI
                     IPCRPEGFSNMFKSSRNIIISDISEKYSQPKVVYARDKWAHPQAIVRITAPNDSSFVE
                     AVQKFGKNILDFFITQERQRQMEYNKSFVNLEARAEIEKIFGIQIDIPSEFKKAVKKK
                     DFYWITNDNPKIRKDIVIYSYPYTDKNTFTKDYLLAKRDSVMKYNVQGEIEGSYMGTE
                     YKYYPPIFREIWVNGGYCAEIRGLWKMFHGAAMGGPFYSHTRLDEINQRVITIEGFVF
                     APGEKKRNAIRQLESVVYSAKLPQEINALKEISVTPKK"
     gene            15091..16149
                     /gene="pdxA"
                     /locus_tag="PLZ54_02190"
     CDS             15091..16149
                     /gene="pdxA"
                     /locus_tag="PLZ54_02190"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOJ65782.1"
                     /translation="MEQKIILGITHGDFNGIGYEVILKALSDPVILDFFTPIIYGSSK
                     AAGYYRKMLDIQNLNLNIIGSVKELNHKRVNIINCVDENIKVEVGQSTPEAGEAAYIA
                     LERAVEDLKNGEIHALVTAPINKKNIQSEKFHFPGHTEYLAQHFGNTENALMLMVNDV
                     MRIAVVTGHIPLREVPSTLTSELVLKKLQILNHSLKEDFMVVRPRIAVLGLNPHAGDD
                     GLIGDEEKTILLPAMKEAEKAGIICVGPYPTDGFFGSGSFTKFDGILAMYHDQGLTPF
                     KTISMDNGVNFTAGLPIIRTSPAHGTAYDIAGQNVASENSFRQALYLACDVYKNRELN
                     HQLKSNQLKFESKEKENG"
     gene            16448..17560
                     /gene="ald"
                     /locus_tag="PLZ54_02195"
     CDS             16448..17560
                     /gene="ald"
                     /locus_tag="PLZ54_02195"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOJ65783.1"
                     /translation="MKIGVPKEIKPQEGRVAMVPAGVMELVHRYGHEVFVQKGAGEES
                     GFSNEAYLSAGAVMLDTIEEVYSMADIIVKVKEPIEREYSLIKRNQILFTYFHFASSK
                     KLTEAMMQSGAVCIAYETVMEKDGSLPLLMPMSEVAGRMSVQQGAKYLEKPQNGKGIL
                     LGGVPGVRPANVLIIGGGVVGTQAAYMAAGLGANVTVLDVNLYRLRYLDDVMPANVIT
                     QYSSKYAISELLPTADLMIGAALTVGAKAPHLITREMLKLMQKGTVMVDVSVDQGGCF
                     ETTHPTTHTDPVYEVDGILHYCVANIPGAVPITSTRALTNATLPYVIKLAELGWEKAC
                     KNHEDIKHGLNIVDGKIVFKNVAESLELPYTEYQIS"
     gene            17562..18539
                     /locus_tag="PLZ54_02200"
     CDS             17562..18539
                     /locus_tag="PLZ54_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOJ65784.1"
                     /translation="MVHWGFIGCGDVTEKKSGPAFQKIEGSDVVAVMSRRLDKAKDYA
                     LRHGVKKWYDDPDKLIHDPEVTAVYVATPPSSHAEYAIKAMKAGKPVYVEKPLASSWL
                     EGQKIVQVAEETHVPCFVAYYRRTLPYFLRVKELIDEGKLGKISSVQIRFALPPSQND
                     FRPDTLPWRVKKEIAGGGYFYDLAPHQFDLLDFILGPISEAQGFTSNIAGLYEVEDAV
                     SGIFRFSNGILGSGMWTFSAPENFRCDEILFVGTKATLTCSTFDFSPIVLENGEKVEQ
                     FSEPKPENIQFYLIESIINFLNGKGTEPVSNCYSAIRTNWVMDTILKKI"
     gene            18739..19230
                     /locus_tag="PLZ54_02205"
     CDS             18739..19230
                     /locus_tag="PLZ54_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOJ65785.1"
                     /translation="MHKILLLMSVIMTLHMSVFSQKSEPSTIVQSDGLFFTDLSQTKL
                     YTGDYREFYDNGSLRLEMYIKDGKPEGDYIIYFPNGKPNEIRSYRNGKFHGTWRTYNE
                     SGVLISEAFYRNDKKHGTWHIWDDNGIMRYEMHYHEGEKIGTWFMWDEKGKLISEKNY
                     STP"
     gene            19339..19686
                     /locus_tag="PLZ54_02210"
     CDS             19339..19686
                     /locus_tag="PLZ54_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ65786.1"
                     /translation="MKKNFFAVVFFALSVFLGGNVMAGENNSSEAKNVSNTKTVIEGK
                     VVDKNTLETLAGVKIMLNGQKVYTDLDGRFTISTFPTGKARLKASLISYEEQDVEIDL
                     STSNSLQIKLAQH"
     gene            20002..20694
                     /locus_tag="PLZ54_02215"
     CDS             20002..20694
                     /locus_tag="PLZ54_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ65787.1"
                     /translation="MNAPTRILVVDDEEDLCEILQFNLETEGFQVDTAYSAEEALKKD
                     LTVYNLILLDVMMGEISGFKMARMLHDNPKTASIPIIFLTAKDTENDKITGFNIGADD
                     YISKPFSLREVIARVKAVLRRSESMQMDETTNKILTFEKLEMHLNNKKVSLEGNEIPF
                     TKKEFEILKLLLENKNRVFSREEILAKVWTDDVCVLDRTIDVNITRLRKKIGPYGKNI
                     VTRLGYGYCFEG"
     gene            20766..22493
                     /locus_tag="PLZ54_02220"
     CDS             20766..22493
                     /locus_tag="PLZ54_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ65788.1"
                     /translation="MKLNKKIFLYFFSIFFILFVIVTYFQYHREKEFSRSQLDGQLNS
                     YVHTIHNFIQSEQPTWEELSRFVRLFPDSTLRVTIVDSLGNVIFDSFLPKGTLFQNHL
                     DRPEIAKARKEKIGEAIRYSASTGKNYYYMAQRFPEYYIRCALPYNVNLIAALRANMF
                     FLYFMLFMFVITVFALFIISKNFSRSIDRLRIFTQKAEQDDILDTDIQFPNDELGEIS
                     NNIVQIYRRMLESKADADRQREKLFKHLQISREGLGIFSSDKKEILANTHFIQYTNIL
                     SDQEVKSSDQIFSISEFSEINAFIDDCFSNNETKRKRITIEKNGHSFYVQCIVFQDDT
                     FEISINDISAQEHENELKRHLTQNISHELKTPVSSIMGYMESILENPNISPQRERFFI
                     ERAYQQTQRLNALLQDISTLNKLDEAYKLFAKEPCSLSEVIDEVLNDVHVQIEQKKCR
                     IIKNYPSDMKINGNNSLLYSIFRNLIDNALTYGGDNITVEINCYREDNEFYYFSFSDN
                     GRGVAEEHLNRLFERFYRVDKGRSRKFGGTGLGLAIVKNAVLFHKGNISAKNQPGGGL
                     SFIFSLRKF"
     gene            22725..22949
                     /locus_tag="PLZ54_02225"
     CDS             22725..22949
                     /locus_tag="PLZ54_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4492 domain-containing protein"
                     /protein_id="HOJ65789.1"
                     /translation="MQRVGFLKNIYSFYSEGFKNMTVGKTLWIIVLIKLFIMFGILKV
                     FFFPDYLNEHFRDDAGKANHVRMELINKSK"
     gene            22973..24532
                     /locus_tag="PLZ54_02230"
     CDS             22973..24532
                     /locus_tag="PLZ54_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HOJ65790.1"
                     /translation="MELLLDASLVNWSRAQFAMTAMYHWLFVPLTLGLGMIMSIMETL
                     YVKTGKEEWKKAAKFWMTIFGINFAIGVATGIILEFEFGTNWSNYSWFVGDIFGAPLA
                     IEGILAFFLEATFISIMFFGWKKVGKNFHLASTWLTTLGATISALWILVANAWMQYPT
                     GMTFNPDTVRNEMIDFWSILFSPVAINKFFHTVISGWVLGALFVVGISCWFLLNKREK
                     DFALRSIRVSALFGLISSLLLLYTGDGSAYNVAQRQPVKLAAMEGLYEGKNGAGLIAI
                     GILNPEKKSYNDTLAPYLFKVEIPKLLSFLGYRDANAFVPGLKDIIEGGYQTASGETL
                     SFEEKRERGKQAIQALADYQQASQKKDTVAAKMYKDRLMKNYAYFGYGYLDHPQQLIP
                     NLSLVFYPFHIMVILGIYFIVLFAGVLILSYRKKLEHLSWLQWICLLTIPLGYVAGEA
                     GWMVAEIGRQPWAIQDILPVQVAVSSISAESVRLTFFLFLILFTSLLVAEIGIMVKQI
                     KKGPEAEKIEE"
     gene            24580..25728
                     /gene="cydB"
                     /locus_tag="PLZ54_02235"
     CDS             24580..25728
                     /gene="cydB"
                     /locus_tag="PLZ54_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005932319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HOJ65791.1"
                     /translation="MITYSFLQHYWWFLIALLGGLLSFLLFVQGGQSMIFSLSKTEDE
                     KRLLVNVLGRKWEYTFTTLVTFGGAIFASFPLLYSTSFGGAYWVWMIILFCFTLQAVS
                     YEFQTKAGNLLGKNTYRFFLFLNGLLGTFLLGVVIATFFTGSDFVVDKANISNPLSPI
                     ISRWQHPWHGLEALLNLRNVCLGLAVFFLSRTLAALYFINRVDHEMLEKRSRKYLWYN
                     AIPFVVFFLAFVIWTFLSDGFAVHPVSGEVYLSPHQYFLNLVEMPAVMTTFLLGIVGV
                     LYGIIRTLFSENYRKGIWFAGAGTVLAVLGLLLLVGFNNTALYPSATHVQSSLTLSNA
                     SSSEFTLKVMSIVSLIIPFVVIYIFYAWRALEKKRTNVEEINEDPHAY"
     gene            complement(25976..27106)
                     /locus_tag="PLZ54_02240"
     CDS             complement(25976..27106)
                     /locus_tag="PLZ54_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ65792.1"
                     /translation="MKIIRRILQDKIIDKIAPGKVVLIFGARRVGKTILMHQILESYQ
                     GKTMLLNGEDYDTLTLLENRTIANYRHLFNGIDMVAIDEAQNIPQIGSILKLIVDEIP
                     GISVLASGSSSFDLLNKTGEPLVGRSSQFLLTPFSHREILQMETMLETRQNLEARLIY
                     GSYPEVVMMDNFDRKTDYLRDIVGAYLLKDILAVDGIKNSSKMRELLRLIAFQLGNEV
                     SYDELGKQLGMSKTTVEKYLDLLEKVFVIYRLGAYSRNLRKEVTKAGKWYFYDNGIRN
                     AIIGAFSPLAIRQDVGALWENYLISERRKANLNEELHKEFYFWRTYDKQEIDLIEESS
                     NDLTALEFKWRNKIPKIPKAFQETYPHAKFYVVNKDNYLEFI"
BASE COUNT         8990 a         4943 c         5551 g         8683 t
ORIGIN      
        1 cttgcctgca aaggaacatc agaatgccat tagtgcctac aatttttata ggctgattca
       61 tcatgcccaa aaggtttatt ttttatataa ttccagtact gaaggttttc agacgggaga
      121 agtcagcaga tttctgtatc agctcgaata tctttacgac acaaaggttg aacgtaaaaa
      181 cattacttac gatatttttt tgaaagatgc agtctccgct tcggtagaaa aggactcact
      241 tatccgcgaa aaattgcagc gttttgtttc ttccggcgat gacaagttgt acctttctgc
      301 cagctccatc aatgcttaca tagattgtcc gctgaaattt tattatgccc aaatagcagg
      361 aatcaaagaa acggaagaaa ttactgaaac agtggaaaat gatttgttcg gtactttgtt
      421 ccactcttct gctgaattgc tttacaaacc atttaaagat gtgcagctgg gaaaagaaga
      481 ttttgagaaa attttgcgtc agccttcgtt ggtggacgag gccatttcac tggctttttc
      541 aaaacatttt tttaaaaacg aacaaaacgg aaaaatcccg ccattagaag gcaataattt
      601 actcatcagc aaaatactga aaaagtttct gatgcgttta ttgtatgtcg atcagcaatt
      661 gacacctttt cgttatctcg gcgctgagga attttgcagt attcgcttcc cgctcgaaac
      721 gctaaacttg cgagtgaata taaaaggcta tatcgatcgg attgatgaaa cggaaaattt
      781 tttgcgtatc attgattata aaacgggaac gggaaataca acctttaaat tgttggatga
      841 tttgtttgat ggttctctcc cgatgaaaaa tcggccaaaa catatttttc aggtttttat
      901 gtattgcctg ctttatcgtt ccaaagccga aaagaaaatt atttcacccg ccatttacta
      961 tttgaaggaa atttttagca gcggtttttc ttcttcaatt caattcaaac cggagaagca
     1021 cactattcag gtggaagatt tcggcatttt cgagcatgaa tttaaggaaa aactgacttt
     1081 attgctgaat gaaattttta atcctgaaat tccttttaag caaacgcaaa ttaaagaaaa
     1141 ctgcgacaat tgttctttta tggaaatttg caatcgttaa aaattttctt tttcgacagg
     1201 gaaaattgca aaagttgttt caaacctgag aagtcgagta tcaaaaatga gtagagaaaa
     1261 catttttctt cgaagttgag catgaaaaaa tttcaaaatc agaatttggt ggaaaaatgt
     1321 gctacagata ttttaaatcg acaaatatct tttaaaaatg aaaattgaaa cagaaagaaa
     1381 atttttggta acgggagcat ttaaatctca agcttccaaa agttatcgca ttattcaggg
     1441 ttacctgtgt tctgatcctg agcgtacggt cagagttcgt attatggata tttctgcttt
     1501 ccttactatt aaggggaaaa gtaatctttc gggattgagc cgcttcgagt gggaaaaaga
     1561 aatccctgtt gacgaggcgc aggaattaat gaaaatttgt gaacccggca ttatcgaaaa
     1621 aaagcgttac gaagttgttt atcggggaca gcgttttgaa gtggacgaat tttttggcga
     1681 aaatgaagga ttgattttag ccgaaattga attggaaaat gaaagccaag aggttttaaa
     1741 gcccgattgg ttgggagaag aagttacagg aaaacctgaa tattacaacg ccatgctcaa
     1801 aaaaatcctt atcgatcgtg gaaaaaatag aacttttaga aagtattttt gtttaatcat
     1861 tatttatgtt tatccaacag ctacaactaa aacttcctta aaccaaaacc gaaaataata
     1921 tgatacattg aaaaacaata gtttgtaaaa atcatcagtt tatgaaaaag tttttttctt
     1981 tgttgagcct ttttttgttc gtgagtatat tgtctcaggc tcaaaacgag ttgtttctga
     2041 aaaaattatt tatttacaaa ggagatacct tgccttatcg gattttatac cccgaaaatt
     2101 acaacaagtc cgcttcttat ccgcttgttc ttttcttgca tggagctgga gaaagaggaa
     2161 atgacaatga aaaacagctg gtgcatggcg cttccctttt tactgattca cagaaccgac
     2221 aaaaatatcc cgccattgta gtttttccgc aatgtcctga aaatggtttt tgggcgccaa
     2281 ttaaagaacg ggacaacgga ttttctttca taaattccaa agagccaaca ccatccatgc
     2341 aaatggttat tcggttactt aaacaaatca ggaaagaaga ggctgtagat agccgaagga
     2401 tttatgttat gggactttcc atgggaggaa tgggaacatt cgacctgatc tgtcgtcagc
     2461 ccaaaacgtt tgcagcagcc attcctattt gtggaggtgt aagtctggaa aggttgaaga
     2521 aagttaaaca catgcccatc agaatttatc atggtggcag cgatgatgtg gtttctccag
     2581 aacattcgcg caatgcttat attgaattaa aagcgcttgg atcgactaaa gttgaatata
     2641 ttgaatttcc cggagtaaaa cataacagtt gggattttgc ttttgctcaa cctgattttt
     2701 taagctggtt gttcagccaa aagaaataac taaaaatcaa ttaattacat tttaaaattt
     2761 ttaaaaacat ttttcattta aagaatttgg ataaaaaaat tccaacaatt ttctcaaatt
     2821 tcactacttt tgcgaagaaa aaaaatttaa aatataaaca gaatggctaa aataaaagga
     2881 actgtggtcg tagatacgga acgctgtaaa ggatgcaatc tgtgcgtcgt agcttgccca
     2941 cccaaagctt tatcgctgtc gaaggaggca aattcaagag gatacaacta ttctttcctg
     3001 agtaatcctg atgcatgtgt tggttgcgtt gcttgcgcct atgtttgtcc cgatgcttgc
     3061 atcacggttt ataaaatgag agtacaataa atttctactc ttcaaccgta tttattcaat
     3121 gtttaaattg taaaaaaatg aacaaaaact caaaaacaga taacaaagaa gaagtccttc
     3181 tgatgaaggg aaacgaagcc attgcacatg cagccatacg ttgtggatgc gatggatatt
     3241 ttggttatcc cattactcct cagtcggaag ttatggaaac cttaatggaa ctgcaaccct
     3301 ggacaacaac cggtatggtg gtattacagg ccgaaagcga agtagcttcc atcaacatgg
     3361 tttatggagc tgcaggttgt ggaaaaagag ccatgacttc ctcatccagt cccggtgtca
     3421 gtttaatgca ggaaggaatt tcctatatgg tgggtgcaga gttaccctgc gtaattgtta
     3481 acgtaatgcg cggaggtccg ggtttgggaa cgattcaacc cagtcagggc gattattttc
     3541 aggcagtaaa aggtggtgga cacggtgatt ataaacttct aacgttagct ccggcttccg
     3601 tacaggaaat ggccgatttt atgccgctgt ccttcgattt ggcttttaag tacaggcatc
     3661 ccgtcatgat tctggccgat ggaattattg gtcagatgat ggaaaaagtg gtgttgcctc
     3721 ctttccaacc acgtcgcacc gacgatgaaa ttcgtgaaga atgtccgtgg gctacgctgg
     3781 gtaagacatc caaccgacca caaaatgtaa tttcttcctt ggcactcgaa gctgaagtaa
     3841 tggaagccat taatttgaga atccagaaac gctatcggga gatggaaaaa aatgaagtcc
     3901 gttacgaaac ttatatggct gatgatgccg attacctgat tgtagctttt ggaacttgcg
     3961 ctcgcgtgag tcaaaaagcg gttgaaatgg cacgcgagaa cggaatcaag gccggtttgc
     4021 tgcgtcccat tacccttttc ccttttccaa aagatattat taatcaattg gcttcgtcgg
     4081 tcaagggaat gatttctatc gaaatgaatg ccggacaaat ggtggaagat gtccgtctgg
     4141 cagttaatgg aaaagttccc gttgaatttt ttggtcgatt gggcggaata gtaccttcac
     4201 ccgatgaagt ttacgatgct ttggtatcca aactgatcaa ataaaaataa ttcttcagcc
     4261 ggttaattca tttgctgaag tttaattaac agtattattc attcaaaaat ttagaaaaat
     4321 gtcgaacaac gaaataatta gtcccgagaa tttggtttat gcaaaaccca agtcactcaa
     4381 agaaacttat atgcactatt gtccgggctg cagtcatgga gtagttcacc ggattattgc
     4441 cgaaataatt gatgaaatgg acattcagga taaaactata ggaatagctc cggtaggatg
     4501 tgccgtattt gcctacaatt atattgacat cgactggcag gaagctgctc acggacgagc
     4561 accggctttg gcaactgcca ttaaaagatt attgcccgat cgttatgtgt ttacctatca
     4621 aggagatggc gatttagctg ctattggaac agccgaaacg attcatgctt gtaatcgtgg
     4681 tgaaaatatt gtgatcattt tcatcaataa cggtatttac gggatgacag gtggacaaat
     4741 ggcaccaacc acactgccgg atatggtaac ttctactact ccctatgggc ggaaaacgga
     4801 attgaatggt tatcctctga atatcaccaa tttgttggca caattgcccg gaacttgcta
     4861 tgttacgcgt caaagcgttc agtcggtagc cggagtgcga aaagcaaaaa aagccattca
     4921 gaaagctttt gaaaattcca tgttaaataa gggaacttcc atagtagaag tagtttctac
     4981 ctgtaacgca ggctggaaaa tgacacctga aaatgccaac aaatggatgg aagaaaatat
     5041 gttccctgcc tatccgctgg gagatttaaa ggacatttaa tcgatgtttc aatactcttt
     5101 aaataccgaa tttctacact gatcataaat tagaataata acaataaatc atattgattt
     5161 taaggtaaac ttatttgttt taaaagagaa agtattattg aaaaaacaat aaacagatga
     5221 aagaagaaat cattatttca ggttttggtg gacaaggcgt gctttccatg gggaaaattc
     5281 tcgcatattc aggcttgatg caaggccagg aagtgagttg gatgccatca tacgggcctg
     5341 aacaacgagg gggaacagca aatgttacgg ttattttaag cgatgaaaaa atcagctcgc
     5401 cggttttgag ctcctatgat actgctatca ttttaaatca gccttcactg gaaagatttg
     5461 aaagcaaggt gaaaccgggc ggtacattga tatttgacgg aaacggattt cacaaattcc
     5521 ctacgcgcac cgatatcaac gtgtatcgaa tcgatgcaac agaatatgca acagctcata
     5581 atgctgccaa aacgctcaat atgattattt tgggaggatt tttaaaagtc aggcccattg
     5641 tgaagataga gaatgtgctg gaaggtttga aaaaatcgtt gcccgaacgt catcatcatt
     5701 tgattccgca aaacgaaaaa gctttgcaaa taggaatgga attgattaaa aaagttaatt
     5761 gacttttttc ctctggtttt gaacatcaat agactttttt tgaaaaaaag catctattga
     5821 tgttctttgt tatttattgt gtaacttttg ataaatcaag acaacaaaaa atttaatact
     5881 tctcttcaaa aaaatctcat tttttctccc ttttgaaaaa aaatcaggtt ttaagagtgg
     5941 cttctttttt tataatttat ttaaacgctc tgtatgttac tttaaatatt gtatctttgc
     6001 aagtgtaaag ctaatttttt aaggtgaata attttacagc tgttttaata actcttttct
     6061 tctccgcttt tttctctggg atggagattg cttttgtttc ttcaaataag cttcgatttg
     6121 agctggataa gaaacagcgc agtataactt catccatact tactattttt tataagcatc
     6181 cccagcagta catttctacc atgcttgttg gaaataatat atctcttgtt atctacgggt
     6241 tgctgatggc aaaaattttg tccccattgc tacaaatact tggtagtcaa tttttaatta
     6301 ctttcacaca aactatagtg gctacagctc ttgttttggt tacgggagaa tttttaccca
     6361 aaagtatttt tcgtgtaaat ccaaattttt ggttaagcat tttttctcct ttgttattgt
     6421 tcttctatgt aattttatac cccattacct tgttagcaac gtctctttcg tttggaatta
     6481 tgagactaat gggtataaaa atcaaccctt tggctaatga aaatgccttc tctcgcgtag
     6541 atttgaatta tttggtacac gaaagttttg aaaataccga acacaagcag gatgttgaca
     6601 acgaagtaaa aatttttcag aatgcacttg atttttcaaa ggttaaatta cgcgattgct
     6661 tggtttcacg taccgaaata gttgcattgg attacaatgt ggatttggaa acgttaaagc
     6721 aaaccttcat tgaaacgggt ttctctaaga ttcctattta caaagaaaat attgacaaca
     6781 tcattggtta tattcattct tctgaaatgt ttcagcatca aaatgattgg agagagcata
     6841 ttaaccagat acccattgtt cccgaaaaca tggcagctca aaaactaatg aaaatttttc
     6901 tgaaagagaa aaaaagtata gcagttgtgg tggatgagtt tggaggaacg gcaggtattg
     6961 ttacattgga agatattatg gaagaaattt ttggagaaat agaagatgag catgacacac
     7021 atgattttgt tgcagaaaag gtcggcgaaa acgaatatgt tttttcaggc cgcctggaag
     7081 ttaaggaaat taatgaaaaa tttcatctca accttcgcga atcggatgct tacgatacta
     7141 ttgccggttt tattctttat catcatcagc attttcctaa gttaaacgag tccatccaaa
     7201 ttgataatta tactttcaaa tgtataaaaa ttaccaataa tcgtattgaa ctggtaaaaa
     7261 tgaaactaca gaaataattt ttttgtcttt ttgaagtcaa gaaattcaat caaaaatcaa
     7321 agtatcaaga atttttcgta tattcgtgcc cttaaaattg aaaaaataat agttttatag
     7381 caaataattt taaatgtaaa atggcaactt tagagaagat caggagcaaa ggagttttgc
     7441 ttattgtagt tatcggactt gctttattgg cttttattgt tggagatttt cttaattcga
     7501 gctccacttt ttttaatcag tcgaaagaaa aaattgccgt cataggtgat gaaaacatca
     7561 gcatccacga gttcagtgca gccattgacc agatgacgga agtgtataaa attgagacag
     7621 ggcaaacgaa tttgaacgaa gaagttgtat cccaattaag agcatcggtg tgggaaaaca
     7681 tgctgatgga aaaaatttta actcacgaag caaaaaaaat agggctttcc gtaagtcccg
     7741 aagaactttc tgaaagactg atcgggaaaa acatacatcc tatcattcta cagcgcgggg
     7801 cttttatgga tgaaacagga caatttagtc gctctttact tgtccaattc ctcaacagtc
     7861 ttgatgttac tcctaccaat aatgagatga agcaacaaat agatcaagct aaaaattact
     7921 ggcttttttg ggaaaaaaac gtaaaaaatg ctattttaca ggaaaaatac aataagttaa
     7981 ttacttctgc catatctgcc aacagtatag atgcaaaaat gaattttgaa gctcgaaaaa
     8041 ttactgttga cgcggcttat gtaatgcaac cgtattattc agtgcctgat tctattttta
     8101 aagtaagttc taaagaactt agagaccttt ataacaaacg aaaagaactt ttcaaacagg
     8161 aaaaatctgc atctatcaat tatgttgccc tggaaatcaa gccttcacaa gaagatttcc
     8221 aagaagctga ggaatggatt aacaagcttg aagacgaatt tagaacaaca gacgatgtgg
     8281 taggtctggt aaataccaat tccgacatta tgtacgacgg cagatattat tctgaagcaa
     8341 ctgtacctgc catgttaaaa gattttgcat ttagagggaa aaaaaatgat gtgttcggac
     8401 ctgtttttga aaacgacact tacactatgg cgcgcatcat ggaaaccgat attcttcgtc
     8461 ccgattcagt taagttacgg catattttcc tgactaagga aaacgaagga aaagctgaca
     8521 gcatagtaaa agcccttcga gcgggtgccg attttgcaac tttggccaga aagtattctg
     8581 ctgttcctca aactgctgca aacggaggtg aaataggctg gttacccgac ggagctcaag
     8641 gagtggataa gcaaattttg gaaaacgcat ttaaaaagcc tctgaatgaa gtttttgtaa
     8701 taaaagatat tcaaggaact caaatcatgc aggtaactga ccgcacgccc ctacgcagaa
     8761 aagtgaaact tgccattctt caaagaaaag ttacaccaag tagtaaaaca tacagtcaaa
     8821 tatacaacca agccaaacaa tttgcagccg aattggatgc agagaaattt gtttcaagag
     8881 cccaagaaaa aggttatatt gtgcaaaaaa ccgaaaatct gaatgaaaat accgaacaaa
     8941 taaacaattt accccaatcc cgtcaaatag ttcgctgggc ctttaaaggg ggaaaaggcg
     9001 atgtttctga tgttttcgat tgcggcaact attatgtagt ggcgatgata accgaaaaaa
     9061 acgaaaaggg ttatatgccg atagaaaaag tttctgctca gttgaaaagc gaaataataa
     9121 gagataaaaa ggcggcgcaa atgataaata atatttcaga gcaattaaag aaaacacctg
     9181 acctcgaagc tttagctgct tcactgaatg cagaaattaa aatggccgaa aacgttaatt
     9241 ttacttctta tcagtttgga ttagccggat ttgaacctta catcatagga aaagctacga
     9301 ttgttccact cgaaaaagtt tcgggaccat ttcaaggaaa tgctggagtt tatgtaatga
     9361 aaacatcaaa caaaaaggaa aataccgaaa cattcaatgc tcagcaggaa ataattcaac
     9421 taaatgcaag aatgctttat tctttgccct atttgatttt agaaaatttg agagaaagga
     9481 ctaaaattga agattaccgt tccaactttt attgaggtat tgaggagttt ttcaatcaat
     9541 atttcgttaa aaaggtattg ttaaataaaa attgtaaaaa cgggatgaaa ctctttcatt
     9601 ccgtttttct tttttatggt cagttttttt aactttgcat tggcaacaaa tgtctgataa
     9661 aacgtttaga taattattgt tgttataaaa acagatattt attattccct atctcattaa
     9721 aaatatattt ccctatgcca attccacttt taggagcttc gttggaggag cttaccgata
     9781 ttgtttcaca actgaaaatg cccacttaca cggcaaaaca aatttcagac tggctttata
     9841 aaaaaagggt agctgatatt aatgaaatgt caaatatttc cgttaaaaac agggacctgt
     9901 taaaagaaaa atataaagtt ggccgtttgg ctcctgttaa agttacagta tcaaaagatg
     9961 aaacgaaaaa atatctgttt cgaacagaaa aagatcattt tatcgaaacc gtttttatcc
    10021 ctgaagaaca tcggaatacc ttgtgtgttt cttctcaaat tggatgtaaa atgaattgtg
    10081 tgttttgtat gactgggaaa caaggattca tggggcaact ttcggcatcg gaaattctaa
    10141 atcagatact ttctgttcct gaagctgaaa aaattaccaa tcttgttttc atgggcatgg
    10201 gcgaaccttt tgacaataca gtagaattat tgcgtacctt aaatatttta actgccaatt
    10261 atggttatgc atggagcccc taccgaatta cggtttccac tataggatta atacccggta
    10321 tgcaaacttt tttggaaaaa tccaattgca atttggctat tagtttgcat tctccttttc
    10381 atagcgagag gctcgaacta atgcccatag agaaagcata tcccatttca caagtggtag
    10441 aagtgcttaa aaattacaat tttggacggc aaagaaaact ttctttcgag tatatcatgt
    10501 tcgataagtt taatgacact atgcgacatg cctcagaatt ggtaagactt ttaaaaggca
    10561 tgaacgcacg ggtaaattta atccgatttc atgccatacc aaatgtttct ttaagaagtt
    10621 caagcgatga acggatgaaa tggttttgcg attatctgaa taataatgga attttggcta
    10681 ccattcgtaa atccagagga gaagatattt cagctgcatg cggactgctt tctacgttgg
    10741 aaaataaaaa aatgtcataa ccaatctttc aaatgagaaa aataatcaat aaaaataaat
    10801 ttcacctgat ttctgaaaaa acgattttgc tatttatcat tgttttagcg ggattattac
    10861 ctaaacccgt ttttgcacaa tcgttattag aaaaacaaat ttcagactcc ctaacctcta
    10921 ttgcgaataa atatacattt gtggagaaaa tatcggtaga taatatttat atcaacaata
    10981 gagaaaaaaa aattattatc aaagccaatg aaaagtttgg ttatatgcct tttagagaag
    11041 aaaatgtaaa acatatctac cgggtactca aacaaatttt tagtccgtcc tacaaaaact
    11101 ataaaatagt ttgtatggtc gacggtcaaa ctatagaaga attaattcca aatttttatc
    11161 gaaccaaaag aaaaagagac gaaaccagaa tttttgatcc aaaaaaacca aagtattcat
    11221 tggtaaaaaa tctttcaaaa ccatataatg taaccaacgg attagaaaga aaaaatattg
    11281 ctctctgggc tagtcatggt ttgtattacg aacaaagcat ttctcgttgg gagtggcagc
    11341 gtgcccgtgt gatgcaaaca gtggaagatc tttttaccct atccattgtt tatccgtatc
    11401 tgatacctat gcttgaaaat gccggagcca ctgtattaac accacgcgaa agagatatgc
    11461 aaagcaatga agttattgta gacaataaca ataaagattc actctcatat cgggaattta
    11521 acctacaaga agtttggaca gaaagccaag attttggatt tgcaaatttc aaaagttttt
    11581 atttacaagg cgaaaatcct tttaacatgg gaagttaccg tcaaatacat tcggtagaaa
    11641 aagagaacaa agcaagtttt gttgaatata tccccgattt tcctaaagca gggaaatatg
    11701 gcatttatgt ttcttataaa acactacccc aaagtactga aaatgctcac tatacggtct
    11761 accacaaggg aggtaaaacc aaattttccg tgaatcagac tatgggtggc ggaacttgga
    11821 tttatctagg acaatttaat ttcgacaaag gaagaaatga aaacggcaaa gtggtactat
    11881 cgaattttac aggggaaaaa gaagaaattg taacagccga tgccgtgaaa tttggtggag
    11941 gaatgggaaa tattgcccgt tctccagatc agaatggaat attggaaaac cataaaagtt
    12001 ctgaaataaa aaatcctgca ttaaagaaag aaaaggtatt gagttgggag actgaacccg
    12061 aaacaagcgg ttatcctcgc tttgcagaag gagcacggta ttggttacaa tgggctgggt
    12121 tccccgacag catctacagc cccaatcgcg gagcaaacga ttatattgat gactttcagt
    12181 caagagctaa atgggtaaat tttctcacgg caggttcttc gctccatccc aaaggaaaag
    12241 gattgaatat tccattggat ttgtctttgg ctcttcatac cgatgccgga acaaccaaaa
    12301 acgattctgt aattggcact ttgggaatat tcacagtcgg caagggtaat gggaaatcct
    12361 ataataataa agtatcgcgt tgggcctcgc gtgatttgac cgatattatt caaactcaaa
    12421 ttgtagaaga catacgcatg agctttaatg atagttggat aagacgcgga ctttggaata
    12481 aatcttacta tgaagccaga gttcctgaaa ttccgactat gttgctcgaa ttactttcgc
    12541 atcaaaatta tgcagacatg aaattaggac tcgatccccg ttttcagttt ttggtaagcc
    12601 gctccattta caaaggaatt ttaaaatacc tgtcggcatt gaatgacgaa gaggattata
    12661 tggtgcagcc cttacccata cgacaattta gctgcatttt tgtagataaa aacaaagtta
    12721 aacttcggtg gaaaacaaga atggattcat tagaacctag tgctgtagca gaacattttg
    12781 tggtgtacac gagaattgac gatggtgact ttgataatgg ggtacttgtg tcggataccg
    12841 aataccttaa ggtgttggaa caagggaaaa tatacagttt taaagtaaca tccgtaaatc
    12901 gtggtggaga gagttttcct tcagaaattt tgtcggtatg ctctcatccg cacgaaaaag
    12961 gcaacgttct catcataaat ggttttgaaa gagtagctcc gcctgccggt tttgttatcg
    13021 ataccaccta tgcaggcttt ttgaacgaca tggatgcagg tgtaccttat ttatatggct
    13081 ataactttac gggaaaacaa tatgaattca gacgtgatag aaaatgggtc gacgatgatg
    13141 caccgggttt cggagctagt catgccaatt atgaaaccca aatactggca ggaaattcct
    13201 ttgattaccc ttttttgcat ggcaaagcta tttgtgccgc cggttattct tttgtttcat
    13261 gcagtgtgga agctgttatg aatgacgata taaatttgaa cgattttcag actgtagatt
    13321 taattttggg aaaacagaag cagactgatg ttggcactag agaaaatatt tctttattta
    13381 aaacttttcc attagcttta cagcaaaaaa ttaatttgtt ttgtcatcag caaggtggaa
    13441 atctactggt tacaggggca tcaattgcgt ctgatatgtt tgacagaaat gacgccttac
    13501 cagacgatat tcgtttttta gaaaacactc ttaaaatcaa atatcgggta catcaagcgg
    13561 ctattacggg gaacgtaaaa tttgtatctt ctccatttaa acaatttgtt cgacatcaat
    13621 tttcttatta ctccgaaccc aatcaatatt cttattttgt cgaatcacct gatgccattg
    13681 aacccgctgc aagggatgct tatacttttt gtcgttatgc agaaaacaat ctgagtgcag
    13741 gagtaattta ttcggggaaa tataaaattt gtgcgttagg atttcctttt gaaaccatcg
    13801 tttcagatgc agaaagagaa gctttcatga agtcagttct ggattttttt gcaaaaccat
    13861 gaagacaaat aatgtcccaa ataaataaaa gatatcaaaa acagaccaat aaatttaagc
    13921 gattttttaa acttaaaaac aaaagaatat gaaacgagca ttcttaagat ttcacgcaag
    13981 aatagtagaa cggattcgcg tgagcaatat ttcaactctt aaatttaatt ttattttgac
    14041 caaaacaaaa acaacatttg ttctgactct acttttaatc atgtcaggtt gcaattccaa
    14101 taaaagttcc ttgccaggaa ttaccggttc aatgtacgaa gttttggttg taatggatca
    14161 tcaacagtgg atttcagcac caggtcgtac gttggttgac ttgttggacc aagacatgga
    14221 agcgctaccg caaccggagc ctgtaatgga tattattcct tgtcgaccgg aagggttctc
    14281 caatatgttc aagtcttcga gaaatattat tatttccgat atttctgaaa aatactctca
    14341 accaaaagta gtgtatgcac gtgataaatg ggctcatcca caagccattg tcagaattac
    14401 ggcacccaat gacagttcgt ttgttgaagc tgtgcaaaaa ttcgggaaaa atattcttga
    14461 tttttttatt actcaggaac gacaaagaca aatggaatac aacaaaagtt ttgtgaattt
    14521 agaagctaga gcagaaatag aaaaaatttt tggtattcag atcgatattc ctagcgaatt
    14581 taaaaaagca gttaaaaaaa aggattttta ctggattacg aacgataatc cgaaaataag
    14641 gaaagatata gtaatatact cctatccata taccgacaaa aacactttta caaaagatta
    14701 cttattggca aaacgggatt cggtaatgaa atataatgtt cagggagaaa tagaaggctc
    14761 ttatatggga accgagtaca aatattatcc accaatcttc agggaaatct gggtgaatgg
    14821 tggttattgt gcagagatta gaggattatg gaaaatgttt catggtgcag ctatgggagg
    14881 tccattctac agtcataccc gtttggatga aattaatcag cgggtaatta ctatcgaagg
    14941 ttttgttttt gctcctggtg agaaaaaaag gaatgccatt cgtcagctcg aatcagttgt
    15001 ttactcagct aaattaccac aggaaatcaa tgcattaaag gaaatatcgg taacaccaaa
    15061 aaaataaatt ttcgattttt acattagaat atggaacaaa aaattatttt aggaatcact
    15121 catggagatt ttaatggaat aggttatgaa gtgatactca aagcattgtc cgatcctgtt
    15181 atcctcgact tttttacacc gattatttat ggttcgtcca aagctgccgg atattataga
    15241 aaaatgctgg atattcaaaa tctgaatctc aacatcattg gttcggtaaa agaattgaac
    15301 cacaaaaggg ttaatattat caattgtgta gatgaaaata ttaaggtcga agttggacaa
    15361 tccacacccg aagccggaga agctgcctat attgccctcg aacgtgcggt agaagatttg
    15421 aaaaatggag aaatccatgc gttggttact gcacccatca acaaaaagaa tatccaatcg
    15481 gaaaaatttc actttcccgg tcataccgaa tatcttgccc agcacttcgg gaacacggaa
    15541 aatgcgctga tgttgatggt caacgatgtg atgcggatag ctgttgtaac ggggcatatt
    15601 ccactacgcg aggtcccgtc cactttaact tcggaattgg tattgaaaaa actgcaaatt
    15661 ctcaaccact cactgaaaga ggattttatg gttgttcgac cacgcatcgc cgttttggga
    15721 ctaaatccac atgccggcga tgatggattg attggcgacg aagaaaaaac tattcttctc
    15781 cctgccatga aggaagccga aaaggccgga ataatctgtg tcggtcccta tccaactgac
    15841 ggtttcttcg gatcgggaag ttttacaaag ttcgacggca ttttggccat gtatcacgat
    15901 caaggcctga cacctttcaa aacaatttct atggacaatg gggtgaattt cactgccgga
    15961 cttcccatta tccgcacttc tcccgctcat ggcactgctt acgacattgc cggacagaat
    16021 gttgcttccg aaaattcttt ccgacaagct ttgtatctgg catgcgatgt ttacaaaaac
    16081 agggaactta accatcagct aaaatcaaat cagctcaaat ttgaaagcaa ggaaaaagaa
    16141 aacggataaa taaaaagttt tacggaaaca gataagcgaa aatggcgaat ctctattgta
    16201 gccgaatcga agttccattt cattaataga aaaggggaaa tgattttgat ttgttttaaa
    16261 acaaaatgaa agttacaaaa aagttaaaaa ttcatttatt taagtgcctt tttgtcggct
    16321 tcattaggcc tgttgctgtt ttcgatttaa tgttcacaaa attatttcaa attgtttggt
    16381 gagaagaagt tgtttgtttg tattgtacac tgtttgttat caaatagcta attaaatata
    16441 aaagattatg aaaataggag ttcccaaaga gataaaaccg caagaaggcc gggtggcaat
    16501 ggttccggcc ggtgttatgg agttggttca ccgatatggt catgaagttt ttgtacaaaa
    16561 gggagcaggg gaagagagcg gattttccaa tgaagcgtat ttatcggctg gagccgttat
    16621 gttggatacc attgaagaag tgtacagcat ggctgatata attgtgaaag tgaaggaacc
    16681 aattgaaagg gaatattctc ttatcaaaag gaatcagata ctgtttactt attttcattt
    16741 tgcttcgagt aaaaaactta ccgaagccat gatgcaaagt ggggctgtat gtattgctta
    16801 cgaaaccgta atggaaaaag atggaagttt gccgctattg atgccgatga gtgaagtagc
    16861 agggcgcatg tctgttcagc aaggcgccaa atatctcgaa aagccacaaa acgggaaggg
    16921 aattttgctg ggaggcgttc ccggtgttcg tccagccaat gtgctcatta ttggtggagg
    16981 tgtagtagga actcaggctg catacatggc tgccggtttg ggagcaaacg ttaccgtgct
    17041 cgatgtcaat ctgtatcggc ttcgttatct cgacgatgtc atgcctgcca atgtaataac
    17101 tcaatactcg agcaaatatg ccatttccga acttttaccc acagccgatt taatgattgg
    17161 tgcagcactc acggttggag ccaaagcgcc gcatctcatt acaagagaaa tgctcaaact
    17221 tatgcagaaa ggaaccgtta tggtggatgt atctgtagat cagggtggtt gttttgaaac
    17281 cactcatccg actacccaca ccgatcctgt ttacgaagtg gacggcatat tgcattattg
    17341 cgtcgccaac attccgggtg cggttcccat aacttctacc cgcgcactga caaatgccac
    17401 attgccttac gttatcaagc tggctgaact tggttgggag aaagcttgca aaaatcacga
    17461 agatataaag cacggtttaa acattgtcga tggcaaaatt gtttttaaaa atgtagccga
    17521 gtcattggaa ctaccttaca ccgaatatca aatttcataa aatggtacat tggggattta
    17581 taggttgcgg tgatgtaacc gagaaaaaaa gtggaccggc ttttcagaaa atcgagggtt
    17641 cggatgttgt ggccgtaatg agccgtcgtc tggacaaagc caaagactac gctttacgtc
    17701 atggagtgaa aaagtggtac gacgatcccg acaagctgat acacgatcca gaagttacgg
    17761 cagtttatgt ggcaacgcct ccttcttctc atgccgaata tgccatcaaa gccatgaaag
    17821 ccggcaaacc tgtttatgta gaaaaacctc ttgcttcttc ctggctggaa ggtcagaaaa
    17881 ttgttcaagt tgccgaagaa acccatgtgc cttgttttgt tgcttattac cggcgaacat
    17941 tgccttattt tttacgtgtt aaagagttga tcgacgaggg aaaacttgga aaaatatctt
    18001 ccgtacagat tcggtttgcc ctaccgccgt cgcaaaatga ttttcgtccc gacactttgc
    18061 cttggcgggt taagaaggaa attgccggcg gcggatattt ttacgatttg gctccgcatc
    18121 aattcgattt gctcgatttt attcttggtc ctatttcgga agcgcagggt tttacttcga
    18181 acattgccgg tctgtatgaa gtggaagatg cggtgagcgg aatttttcgc tttagcaacg
    18241 ggattttagg tagcggaatg tggacttttt ctgctcccga aaattttcgg tgtgacgaaa
    18301 ttctttttgt aggaacaaag gcaacactca cctgttcaac tttcgatttt agtccaatag
    18361 tgcttgaaaa tggagaaaaa gtagaacaat tttctgaacc aaaaccggaa aacattcaat
    18421 tttatttgat agaaagtatt atcaattttt tgaacgggaa aggcactgaa cctgtaagca
    18481 attgctattc ggccattcga accaattggg tgatggacac catattgaag aaaatttaaa
    18541 cttcaattaa ttttaaatca agtaaaaatt cataagacac ttattatttt tatcttgcta
    18601 agtcattttc gctttctact aaaacttgag aaattttttc tgttttttgt aacatgtatt
    18661 aaattttttg atgatgaaaa tttttcacat atatttcact tacatttgta gaggtattaa
    18721 cgataaaaaa tataagttat gcacaaaata ttgttattga tgtcggtaat aatgactctc
    18781 cacatgagcg ttttttcaca aaaaagcgaa ccatctacca tagtgcaaag cgatggtttg
    18841 tttttcaccg atttgtccca aaccaaactt tatacagggg attatcgcga gttttacgac
    18901 aatggatctt tgcggctcga aatgtacatc aaggacggta agccggaagg agattacatt
    18961 atttatttcc ccaacgggaa gcccaacgaa atacgttcct accggaacgg aaaatttcac
    19021 ggaacatggc gcacatacaa cgaatctgga gtactgatat ctgaagcttt ttaccggaac
    19081 gacaaaaaac atggtacttg gcatatatgg gatgataatg gtatcatgcg ctatgaaatg
    19141 cattatcatg agggcgaaaa aatcggaacg tggtttatgt gggacgaaaa agggaaatta
    19201 atttccgaaa aaaactattc taccccttaa ttttttcatg tatttgtatt ttttttgtaa
    19261 tactattgtc atatacagta acttactttg tatccaaaca aaggggatta atttcaaaca
    19321 aattaaaata caaatcaaat gaaaaaaaat ttttttgctg tagttttttt cgcattgtcg
    19381 gtttttctcg gaggcaatgt tatggccgga gagaataatt cttcggaagc aaaaaatgtt
    19441 tcgaacacaa aaactgttat cgaaggtaag gtagtggata aaaataccct tgaaacattg
    19501 gcaggcgtaa aaataatgct gaatggtcaa aaagtttata ccgatttgga tggtcgtttt
    19561 acaatttcta catttccaac cgggaaagct cgattgaaag caagtttgat ttcatacgaa
    19621 gaacaggatg tggaaataga tctgtcaaca tccaattcac ttcaaattaa gttggcacaa
    19681 cattgacatt ttttttaaat cttttttgca aaggcagttc tttttcgaat tgcctttttc
    19741 aatatgtaca ttcccgaaaa ttcctgtttc ctcttttaaa gtccaatata tgaatcgatt
    19801 gtattttcca tcggtaaaag tataattatg ccgaattcta aattcaacta ttttagtcag
    19861 agaattcaat ttttgtcata tatttgtaat atatattaca taatatcgaa ataattcttc
    19921 agttactttg caacatgaat aaaatttttg ctccaaagtg agcataaatt ggttaattta
    19981 ttatttctaa agtagaaatt tatgaatgct cccacacgta ttttagtagt agatgatgaa
    20041 gaagatttgt gtgaaattct tcaatttaat ctggaaacag aaggtttcca agtagatacg
    20101 gcatattctg ccgaagaagc tttaaaaaag gatttaactg tttataacct tattttgctc
    20161 gatgttatga tgggtgaaat ctcgggtttc aaaatggcca gaatgttgca cgataacccg
    20221 aaaacagctt ctattcccat tatattcctg acagcaaaag acacggaaaa cgataaaata
    20281 acaggattta atattggtgc cgacgactat atttccaaac cattttcttt acgggaagtt
    20341 atagcaagag taaaagcggt gctgagaaga tccgagtcga tgcaaatgga tgaaaccaca
    20401 aataaaattc ttacttttga aaaactcgaa atgcatctca acaacaaaaa agtatcgctg
    20461 gaaggaaatg aaattccatt tacaaagaaa gaatttgaaa ttttaaaatt gcttctggaa
    20521 aacaaaaaca gggtattttc gcgtgaagag attcttgcaa aggtttggac agacgatgtt
    20581 tgtgtgcttg atcgcaccat tgatgtaaat attacccgat tacgaaaaaa aatcggacct
    20641 tacggaaaaa atatcgttac ccggttgggt tacggctatt gttttgaggg ataaacagat
    20701 gattttattt ggttttagat gaggtgtttt tttaactctt ttccttgcaa attcgacgac
    20761 cctgcatgaa actaaacaaa aaaattttcc tatacttttt cagtatattt tttatccttt
    20821 ttgtaatagt tacctatttt cagtatcatc gcgaaaagga attcagcaga tcgcaactgg
    20881 acggacaact gaacagctac gtgcatacca tccataactt cattcaaagt gaacaaccga
    20941 cttgggaaga actcagcaga ttcgttcgcc tgtttcctga cagcacactc agagtaacca
    21001 ttgtcgattc tttgggtaat gtaatttttg attcctttct gcccaaagga actcttttcc
    21061 aaaatcattt ggaccgcccg gaaatagcaa aagcccgcaa ggaaaagatt ggagaagcca
    21121 tacgttattc tgcttccaca ggaaaaaact attactatat ggcccaacgt ttccctgaat
    21181 attacatacg ttgcgctctc ccttataatg tcaatctcat tgccgctctt agggcaaata
    21241 tgtttttctt gtattttatg ttattcatgt ttgttattac agtttttgca ttgtttatca
    21301 tttcgaaaaa tttttcacgc tctatcgaca gattgcgaat atttactcaa aaagccgaac
    21361 aagacgatat tctcgatacc gacattcagt ttccgaatga tgaattaggc gaaatcagca
    21421 ataatattgt tcaaatctat cgacgtatgc tggaatcgaa agctgatgcc gatcgtcaac
    21481 gggaaaaact gtttaagcac ttgcaaatct cacgcgaggg attgggcatt ttctcttccg
    21541 acaaaaaaga aattctggca aacacacatt ttattcaata taccaatatt ctttccgatc
    21601 aggaagttaa aagttctgat caaatttttt ccatttccga attttccgaa ataaatgctt
    21661 ttattgacga ttgcttttct aacaatgaaa caaaacgaaa acggattacg atagaaaaaa
    21721 atggacattc tttttacgtt cagtgtattg tatttcagga tgacacgttt gagatatcca
    21781 taaatgatat ttctgcccag gaacatgaaa atgaacttaa aaggcatctt acacaaaata
    21841 tctcccatga actgaaaacg cctgtaagta gcattatggg ttatatggaa agtattcttg
    21901 agaatcccaa tatctcacct caacgtgaac gtttttttat cgaacgagct tatcagcaaa
    21961 cccagagatt aaatgctttg cttcaggata tatctaccct caacaagctc gacgaagcat
    22021 acaaattgtt tgcaaaagaa ccgtgctccc tttctgaagt cattgatgaa gttctcaatg
    22081 acgtacatgt ccaaatcgag caaaaaaagt gcagaattat aaaaaattat ccttccgaca
    22141 tgaaaataaa cggtaataat tctttactgt actctatttt tcgcaattta atagacaatg
    22201 ctttgaccta tggtggtgat aatattacgg ttgagataaa ttgttaccgt gaagataacg
    22261 agttttatta tttctcattt tcagacaatg gacgtggtgt agctgaagag catttaaaca
    22321 gactatttga aagattctat cgcgtggaca aaggccgaag tagaaaattc ggcggcacgg
    22381 gtttgggatt ggccattgta aaaaatgcag ttttatttca caaaggaaat atctcagcta
    22441 aaaaccagcc gggaggaggt ctaagtttca tattttcttt aagaaaattc taacccactg
    22501 attcctaata atttttattt tctactgctc gatttttttg agggctaata tactgttgca
    22561 aattttcggt taaaatagag taaagtaaac tgttttacga atgtttgcag gtgcgtattt
    22621 taattttttt aatctaaaat ttgaatgata aagaaattga aagaaaaaat caggctattg
    22681 cttgtaaatc tgaacaaatt gttatttttg tcaaaaaaat atttatgcag agagtcgggt
    22741 ttctaaaaaa tatttattcg ttttattccg aaggttttaa aaacatgacg gtgggaaaaa
    22801 ccttatggat aatcgttttg ataaaactgt tcatcatgtt tggcattctg aaggtttttt
    22861 tctttcccga ctatcttaat gaacatttca gggatgatgc cggcaaggcg aaccatgtgc
    22921 gcatggaact tatcaacaaa tcgaaataga aagaatcaat aaactgaaat ttatggaatt
    22981 attactggat gcatccttag taaattggtc gcgggcacag tttgccatga cagccatgta
    23041 tcactggctg tttgtcccgc tcacattggg tttaggcatg attatgtcga ttatggaaac
    23101 tctttatgtg aaaacgggga aggaagaatg gaaaaaagca gcaaaattct ggatgaccat
    23161 tttcgggatc aactttgcaa ttggggtagc aaccggcatt atcctcgaat ttgaatttgg
    23221 caccaactgg tcgaactaca gctggtttgt aggcgatatt tttggagctc cgctggccat
    23281 agaagggata ttggctttct ttttggaagc cacatttatt tctatcatgt tttttggttg
    23341 gaaaaaagtg gggaaaaatt ttcatttggc ttctacatgg ctaaccacac tgggcgcaac
    23401 tatttcggca ttgtggattc ttgttgccaa cgcttggatg caatatccta cgggaatgac
    23461 atttaatccc gatacggttc gcaacgaaat gattgatttt tggagtattc tcttttctcc
    23521 tgttgccatt aacaaatttt ttcacacggt tatttcgggc tgggtattgg gtgcattatt
    23581 tgttgtgggt atcagctgct ggtttttgct caataaacgc gaaaaggatt tcgctttgag
    23641 aagcattcgc gtcagtgctt tattcggttt gatttcttct ttgcttctgc tttataccgg
    23701 agatggctcg gcttataatg tggctcagcg tcagccggtg aaactggcag ctatggaagg
    23761 actttatgaa gggaaaaatg gtgcaggcct gatagccatt ggtattttga atcccgagaa
    23821 aaaatcatac aacgacactc ttgctcctta tctgtttaaa gttgaaatcc caaaactcct
    23881 ttcatttctg ggctatcgcg atgccaatgc ttttgtgccg ggattaaaag atattattga
    23941 aggtggatat caaactgcat cgggagaaac attatcgttt gaagaaaaaa gagagcgcgg
    24001 taaacaggcc attcaggcac tggccgatta tcagcaagca agccaaaaaa aagatacggt
    24061 tgcagccaaa atgtataagg acaggctcat gaaaaactat gcctattttg gttatggtta
    24121 tctggaccat cctcagcaat tgatcccgaa tctgtcgctg gtattttatc cattccatat
    24181 tatggtgatt ttgggaattt attttattgt gctttttgcg ggggtactta ttttatctta
    24241 tcgaaaaaaa ttagagcatt taagctggtt acaatggatt tgtttgttga cgataccatt
    24301 ggggtatgta gccggagaag ccggttggat ggttgccgaa attggtcgtc agccttgggc
    24361 gattcaggat attttgcccg ttcaggttgc cgtgtcgagc atttcggccg aatcggtaag
    24421 actgactttc ttccttttcc tgattttgtt tacttcttta ctcgttgccg aaatcggtat
    24481 tatggtcaaa caaataaaga agggaccgga agctgagaaa atagaagagt aattttttag
    24541 gagaaaattc attttgtctt tccttaaatt tagaatacta tgataactta ttctttttta
    24601 cagcattact ggtggtttct gattgctctg ctgggaggtc ttctatcttt tctattgttt
    24661 gtacagggtg gtcaatccat gattttttcg ctttcaaaaa cagaagatga aaaacgactt
    24721 ctggtaaatg ttctgggacg gaaatgggaa tatactttta cgactcttgt tacatttggc
    24781 ggcgccattt ttgcttcttt tccgctgctt tactcaacca gttttggtgg tgcttactgg
    24841 gtgtggatga ttattctgtt ttgtttcacc ctgcaggcag tttcttatga atttcaaacc
    24901 aaagcgggaa atctgctggg aaaaaatacg tatcgttttt ttctttttct gaatggatta
    24961 ttgggaactt ttttattggg cgtggtgatt gccacgtttt ttacgggttc ggattttgta
    25021 gtggacaaag caaatatttc caatccgctc tcgcctatta tcagccgttg gcagcatccg
    25081 tggcacggac tggaagcttt gctcaatttg agaaatgttt gtctgggact ggcggttttc
    25141 tttctgtcgc gcacactggc ggctttgtat tttatcaatc gggtggatca tgaaatgctg
    25201 gaaaaacgtt cacgaaaata cttgtggtac aatgccattc cttttgtagt tttcttcctt
    25261 gcatttgtta tctggacttt tctttccgat ggttttgccg tacatcctgt ttcaggtgaa
    25321 gtatatttaa gtcctcacca atattttctc aatctggtgg aaatgccggc agtgatgaca
    25381 acatttttac tgggtatagt gggtgttttg tatggaatta ttcgcacgct gttttcagaa
    25441 aattacagaa aaggaatttg gtttgcagga gctgggactg ttctggcggt gctgggactt
    25501 ctgttgctgg tgggctttaa taatactgcg ttgtatcctt cagcaacaca cgtgcaaagt
    25561 tcgctaaccc tttccaatgc ttcttccagt gaatttaccc tgaaggttat gtccattgtt
    25621 tcgctgatta ttccgtttgt ggtaatttat attttttatg cttggcgggc acttgagaaa
    25681 aagcgtacca atgtggagga aatcaatgaa gatccacatg cttattgaga aaaaggggaa
    25741 atagattcgg taaattttca attcgttgaa atgaagttta ttttttgaac ttcatttaat
    25801 tcgttgaatg attgataatc cggagatttt atttttaata cgacgtggtc ggaaggctac
    25861 gccgaattat gggctatacg gaaaagagaa acccgcttga aaaaatgtgt caatgtatta
    25921 taatacggag atgtagtttt ttcaaaactt atttttgttg gaaatatcct tgaaattata
    25981 tgaattccag ataattatct ttatttacca cataaaactt tgcatgagga tatgtctctt
    26041 gaaaggcttt tggaattttc ggaatcttgt ttctccattt gaactccagt gctgtcagat
    26101 catttgaact ttcttcaatt aaatcgattt cctgtttatc gtaagtacgc cagaaataaa
    26161 actctttgtg taattcttca ttaagatttg ctttacgtct ttcgctgatg agataatttt
    26221 cccataatgc acctacatcc tgtcggatag ccagcggcga gaatgctcct ataatggcat
    26281 ttcggatacc gttatcataa aaataccatt tcccggcttt cgttacttcc ttgcgcagat
    26341 tacgagaata agctcctaaa cgataaataa caaatacttt ttccaaaaga tcaagatatt
    26401 tttcgacggt cgttttgctc atgcctaact gtttccctaa ttcgtcgtag gaaacctcgt
    26461 tgcctaactg aaatgcgatc agccgcaaca gttccctcat cttactagaa ttttttattc
    26521 cgtcgacagc caaaatatcc tttagtagat aagctccgac aatatcacgc aggtaatcgg
    26581 tcttgcgatc gaaattatcc atcattacta cttccggata tgaaccgtaa atcagccgag
    26641 cttcgaggtt ctgtcgtgtt tcaagcatgg tttccatttg caatatttcc cgatgagaaa
    26701 aaggtgtaag caaaaattgc gaactgcgtc cgaccaatgg ttcacccgtt ttgttgagca
    26761 ggtcgaatga cgaagaacca ctcgccaaaa cgcttatgcc gggtatttca tcaacaatca
    26821 atttcaaaat gctgccgatc tgtggaatgt tctgagcctc gtctatagct accatatcaa
    26881 ttccattaaa caaatgtcgg tagttggcta tggtacggtt ttccaacaat gtcagcgtat
    26941 cgtagtcttc gccgttgagt aacattgttt taccttgata agactccaga atttgatgca
    27001 ttaaaatcgt tttccctaca cgccttgctc cgaaaatcaa cacgacctta ccgggtgcga
    27061 ttttatctat tatcttatct tgaagtatcc ttctaataat tttcatacta tttttttgtt
    27121 gcaaatataa aaaaagttat tcaactggca attttttcat aaaaaaaagt attttcaact
    27181 ggcgattttt acaaataacc cataataaat tgaataccaa gttagatttt caatacggca
    27241 gcacattact tacaaagctt aaatttgcgg taaaccttga aaataatggc aaatcctttg
    27301 tggcggggat ttttttattg agtcaatgtt ggttgttttt cttcaatatc atgtccatat
    27361 ctcctccata ccttgtccat gtttccttcg taccatgttc ttatcttctc catattcaga
    27421 tcatactttt tatttgaaca gaatgatgat tttctctaac tctataaaat gctttgttca
    27481 taatagtgcc ttttattgaa aattaatatg aaacccacat gtattgcata cacaaacacg
    27541 aatgaatata atattcagac atgtgggttc catcagacct taaaaaaata aattatattc
    27601 tgatgaaatg acatcattcc ttcaacctta ttttgctcaa accttaataa caagaacact
    27661 ccttccacta tccaaaacct tattagcata ttaaaaatct ggaaattttg tttttactta
    27721 tgacgtagcc tggaggctac gccgaacaaa aaggtaaaaa tatggggctt tgcccaaaaa
    27781 ttccactttt atatgagccg ttgcatgcaa cggctctact gaaagaatgg acgtttgttg
    27841 gctaaaatct tttaaattcg ttccttgcgg agctcaaaca gaaaagattt tttaacgcca
    27901 acttctctta tttttcggct caccggctaa tgtcaagacc aaaaaataag attgccctgt
    27961 gggtatttcc ttcaacctta ttttgctcaa accttaataa catgaacact ccttccacta
    28021 tccaaaacct tattagctta ttaaaaatca gagattttgg cacaggcaaa tgccttcacc
    28081 agcaaaaaga acgacgtggc cgggaggcta cgccgaacaa aaaggtaaaa atatgggcat
    28141 tgcccaaaaa ttctactttt atctgag
//
LOCUS       DAOSCH010000013        27596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1264_length_27596_cov_6.254421, whole genome shotgun sequence.
ACCESSION   DAOSCH010000013 DAOSCH010000000
VERSION     DAOSCH010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 27596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27596
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1264_length_27596_cov_6.254421"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="PLZ54_02245"
     CDS             complement(<1..796)
                     /locus_tag="PLZ54_02245"
                     /inference="COORDINATES: protein motif:HMM:NF021607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HOJ65793.1"
                     /translation="MKKFFYSFGSCLPAFSLAMFLFNFSLILQVSAQGAGKALNFNGT
                     HKYVSVANSSTLNVSNAMTLEAWMKSAEENFSYRVPITLNPATPIADYQVKVELTTSN
                     FAYAHANSDGSDIRFYDGNGTELNYWMEKWDASGTSIIWVKVPTAGTNEIFMHYGNSS
                     AQSASNATNTFVRVINSNSPVKGSWHLDEGSGTTAYDRSGNGNNGTLYNSPTWVDGKF
                     GKALSFNGSNDYVTTPIKSNTLTDFTMEAWVKASSVPGDRRIVMGSG"
     gene            1636..3885
                     /locus_tag="PLZ54_02250"
     CDS             1636..3885
                     /locus_tag="PLZ54_02250"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HOJ65794.1"
                     /translation="MIFDEAMIQQFYESFPEKIEQIKNRLGRPLTLTEKILYTHLSAD
                     ETLKNFVRGIDYVNFSPDRVAMQDATAQMALLQLMNSGRSKTAVPATVHCDHLIQAHV
                     SAENDLDTAIKTNSEVYNFLRAVSNNFGIGFWEPGSGIIHQVILENYAFPGGLMIGTD
                     SHTPNAGGLTMLAVGVGGADAVDVMSGLPWELKMPKIIGVKLTGKLSGWTSPKDVILK
                     LTGMLTVKGATNAVIEYFGEGTASLSATGKATICNMGAEVGATSSIFPFDNKSAVYLE
                     ATGRNSIAETAKKLAEHLQADPEVVQHPEKYYDRLIEIDLSTLEPHINGPFTPDAAYP
                     ISIFGKVVREKNYPQKLEVGLIGSCTNSSYEDLSRAVSVLRQAREKNLKVRSQMIINP
                     GSEKIRSTAERDGMLDEFKSSGATIMANACGPCIGQWKRDIDVADRPNSIITSFNRNF
                     AKRNDGNPNTHTFLSSPEIVAALSIAGDLCFNPLTDELENEKGEKIKLDEPQGFELPL
                     LGYEIKESGYIAPSEEKMEINIDPKSERLQLLQPFPAWDGKDMIELPLLIKVKGKCTT
                     DHISMAGPWLRFRGHLDNISNNLLMGAVNACNDKTNAVLDPETKQYTTVSALARKYKA
                     KGTGSVVVAEENYGEGSSREHAAMEPRYLNVKAIIAKSFARIHETNLKKQGMLALTFI
                     DKSDYDKIREDDRISILGLSDFKPGKNLLIQLHHSDGSVEQFEVAHSFNKTQIEWFKA
                     GSALNAMRK"
     gene            3976..5331
                     /locus_tag="PLZ54_02255"
     CDS             3976..5331
                     /locus_tag="PLZ54_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005790680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOJ65795.1"
                     /translation="MKEELLIYKLSETIKKTSQIDKELFTKYNVKRGLRNEDHTGVLV
                     GLTRIGDVVGYERTEDGQLKAIPGKLYYRGIDVEELVHGIQSENRLGFEETAFLLLSG
                     YLPTKDELETFKKILNEAMPLQHQATMNIMALKGHDIMNILARCVLEQYIYDEDPDNI
                     SRDNLIRQSINLVSKFPTIIAYAYNVLRHHEKGRSLHVRHPDENFSFAENFLFMIKGK
                     GQYTPLEIKILDLALILHAEHGGGNNSTFSVRVTSSTETDTYSSIAAGIGSLKGPLHG
                     GANLKVMGMIKEMRENINDWTDADEIDQYLKKLLNKEAYDKTGLIYGIGHAVYTISDP
                     RAILLKDMARELAKEKGLEKEFAFQELIEQRAVEVFMNYKNGNNNKQVCVNVDFYSGF
                     VYEAIGIPKEVYTPIFAMARIVGWCAHRIEELNFTSKRIIRPAYKNVNEIQQFIPLEK
                     R"
     gene            5391..6617
                     /locus_tag="PLZ54_02260"
     CDS             5391..6617
                     /locus_tag="PLZ54_02260"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HOJ65796.1"
                     /translation="MKKIKVEQPVVELDGDEMTRIIWRIIKEQLILPYLDIDIKYYDL
                     SIQNRDLTNDEVTIEAAHAIKKYNVGIKCATITPDEARVKEFGLKKMWKSPNGTIRNI
                     IGGTVFREPIICSNIPRLVPNWTKPIVIGRHAFGDQYKATDVPIKGKGTLKMTFTAEN
                     GETQEWEVYNFQGDGVAMSMYNTDESIYGFARSSFQAALDKKWPLYLSTKNTILKTYD
                     GRFKDIFQEVYEKEFREAFQNAGITYEHRLIDDMVASVLKWNGGFVWACKNYDGDVQS
                     DSIAQGFGSLGLMSSVLITPDGKTIEAEAAHGTVTRHYRLYQQGKETSTNPIASIFAW
                     TRGLAHRGKLDGNNALIDFAKKLEEVCVQTVESGKMTKDLAILVHGEKVSRTDYLSTE
                     EFLTALKQNLEAKLSA"
     gene            complement(6709..8994)
                     /gene="rpsA"
                     /locus_tag="PLZ54_02265"
     CDS             complement(6709..8994)
                     /gene="rpsA"
                     /locus_tag="PLZ54_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOJ65797.1"
                     /translation="MEENQINLEKNESALTENVSPEAVEAKKEETQEVNEVLSPEEES
                     VAESHAAEPIVAETPEVSVPEEPVKESVAPVQAEITPEEFDWDAYEKGNTLTTEAKKE
                     LENVYDKTLNAVKDKEVVEGTVISINKREVVVNIGYKSDGVVPMSEFRYNPDLKVGDK
                     VEVYIESQEDKKGQLLLSHKQARISRAWDRVNAALESQEVVKGFIKCRTKGGMIVDVF
                     GIEAFLPGSQIDVKPIRDYDAFVNKTMEFKVVKINQEFRNVVVSHKALIEEELEQQKK
                     EIISKLQKGQILEGVVKNITSYGVFIDLGGVDGLIHITDLSWGRINHPSEVVSLDEKI
                     NVVILDFDEEKKRIALGLKQLTPHPWDALDPNLKVGDKVKGKVVVITDYGAFVEIAPG
                     VEGLIHVSEMSWSQHLRSAQDFLKVGDEVEAIILTLDRDERKMSLGIKQLKSDPWENI
                     EERYPVGSKHVAKVRNFTNFGVFVEIEEGVDGLIHISDLSWTKKIKHPSEFTQIGSEI
                     EVVVLDIDKENRRLSLGHKQLEENPWDALESVFAVDSIHDGVVVEMLDKGAVIALPYG
                     VEGFASAKQLVKEDGTQAQVDEKLPFKVIEFNKDAKKIVLSHTRIHEDLKRAAKTESA
                     EGETSTSPKKSVTKRTKKEESSTVQTQAIEKTTFGDIQSLVDLKDQMESEAAKKLAKK
                     ETKSKKAEPAEKEEPAEVKMAEETKVEEPSEKPAETEEKSEKPQKSSKKEKTEKVEEK
                     VVEAEETPASEEAETSEKSIE"
     gene            complement(9112..9831)
                     /locus_tag="PLZ54_02270"
     CDS             complement(9112..9831)
                     /locus_tag="PLZ54_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOJ65798.1"
                     /translation="MKSKYFVNILIMTMLCFYMQQLNAQDWPNLGRFRAENEKLGAPD
                     KGEKRVVFLGNSITEGWINIRPDFFKNRSYINRGISGQTTPQMLLRFRQDVIKLQPAV
                     VVILAGTNDIAGNTGPSTLEMIEDNLSSMSELARVHNIKVVLSSVLPAYDYPWRRGLQ
                     PAEKIVTLNAWIKDYCQKNGFVYLDYFSAMADERNGLKSEFSEDGVHPNQKGYQVMEP
                     LAEAAIQKALKQNFKKCPCNK"
     gene            10116..11393
                     /locus_tag="PLZ54_02275"
     CDS             10116..11393
                     /locus_tag="PLZ54_02275"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOJ65799.1"
                     /translation="MKTHTKKFYFPNFEKIICLFLLLTGFFSKTLSQDFAKGADVSWV
                     TQMEAAGKKFYTAEGVETECMTLLKSLGMNTIRLRVWVNPADGWCNAQDVLLKAQRAN
                     DLGMRIMIDFHYSDSWADPGKQTKPAAWSTLSFADLKTALSQHTNEVLTLLKNNDITP
                     EWVQVGNETSDGMLWEDGRISKNPQNYAELNNAGYDAVKSVFPNAKVIVHLPNGYSNS
                     LYRWFFDTLKNYGGKWDVIGMSVYPFWYTTKNDWRNCNISCFNNMNDMVSRYGTEVMI
                     VECGMQWDAPEACRDFLSDLINKTKNVSQGKGLGVLYWEPQCYPGWNGYTLGAFDNTG
                     KPTIALDAFKEVSQSTHPLQLKTGWRYDDLQKVILFDKTFSKVTLFDSAGTAIQSFAS
                     TNRISMKNMQKGIYILHAALKNTEPLLIKFIYN"
     gene            11499..13964
                     /gene="priA"
                     /locus_tag="PLZ54_02280"
     CDS             11499..13964
                     /gene="priA"
                     /locus_tag="PLZ54_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOJ65800.1"
                     /translation="MKYIEVILPLPLPNTFTYAVPEADENSIQQGMRVVVPFGKKKLY
                     SGIVYYVHILRPETTYQIKEIVGILDEEPILRRPQIKFWEWIANYYQAHLGEVYQSAL
                     PAGLKLESETLVRVKPDFDTNVHFSEREKKILDVILNQKKPISLSALAKDSGISNVLP
                     QLKLLLEKGVIEISEQLTEKFHLKTETYIRLRPEIQQDEKLHEIFDQLKSAKKQLSLL
                     MNYIDLSKCLDPKNVREVSRKTLLEKSGISPAVLKALTDKKILETYEKIVGRLDFSEN
                     ELQKPSILNEFQTKALQEIEKKFKEKPVVLLHGVTSSGKTELYIHLIQKVLNEGKQAL
                     YLVPEIALTTQLTARLKRIFGKKLGVYHSKFSDAERTEIWNNLLNDKGYEVIIGVRSS
                     IFLPFRQLGLVIVDEEYETSYKQFDPAPRYHARNAAIVLASMHGAHTLLGSATPAIES
                     YFNAVTGKYGLVKLDRRFEEISLPEIVVVDLKEAYHKKQMNGHFSDLLMHEMQKTLQN
                     KEQIILFQNRRGYAPYLECKSCNYIPKCKNCDVSLTYHKTTNALTCHYCGYTESYPAS
                     CPVCGTPAAFSTHGFGTEKIEDEIKKIFPEVRISRMDLDTTRSRKSYETIITDFEEYK
                     IDILIGTQMITKGLDFDKVSLVGILNADNILNFPDFRSHERAFQLLSQVSGRAGRKDK
                     QGKVILQTYQPKHPIIQQVMTNDYEGMFANQCEERKQFKYPPYTRLILLIVRHHDRQI
                     LQEASHQLTNSLRSVFGRRVQGPSDPLIPKIQNFYIKHILLKIEIEASPEKTKKLIRD
                     CINLSLSQPSFKSVHLQIDVDPL"
     gene            14480..15871
                     /locus_tag="PLZ54_02285"
     CDS             14480..15871
                     /locus_tag="PLZ54_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOJ65801.1"
                     /translation="MKNKYIDLIEQTFEFPNDEFNVIDNELWWCGVPLMDVIKQYGTP
                     LRIAYLPKIAENIQKARRMFNVAMAKVDYQGDYMYCYCTKSSHFSFVMEEVLKHDVHL
                     ETSSAFDINIMESLYEQGLLKPETYIICNGFKRPQYIENIQNLIRLGFSNVVPILDNK
                     FELDLLLKDFDKKLKIGIRIAAEEEPKFDFYTSRLGIRYNDIIPYYTKHIANNPQVEL
                     KILHFFINTGVRDTAYYWNELSKAVNLYCELKKKCPELDSLNIGGGFPIQTHLDMSYD
                     YEYMAEEIIAQIKNICTQNGVPEPNIFTEFGSYTVGESGAMLYSIVNQKKQNDRELWD
                     MIDSSFMTTLPDTWAINQRYVFLAINNWDSEYERVNLGGLTCDSEDFYNAEVHSNAIF
                     LPKMEEGREQYIGFFHTGAYQESLSGYGGIQHCLIPAPKMVIIDKDEDGEYFTKLFAK
                     EQSYKSMLKILGY"
     gene            complement(16013..16996)
                     /gene="pfkA"
                     /locus_tag="PLZ54_02290"
     CDS             complement(16013..16996)
                     /gene="pfkA"
                     /locus_tag="PLZ54_02290"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOJ65802.1"
                     /translation="MKYEIKCIGLLTSGGDAPGMNAAIRAVTRTAIFNGIRVKAIYRG
                     YKGLITGEIKEFKTENVSNIIQQGGTIIKTARCAEFMTPEGRKMAYETMRKEEIDALI
                     VIGGDGTLTGARIFAQEFNVPIVGLPGTIDNDLWGTDVTIGYDTALNTIIDAVDKIRD
                     TASSHERLFFIEVMGRDAGFLALSSALASGAEAAIIPERETKVDQLEELIKNGFRKSK
                     NSSIVIVAESEITGGANGLAERMRREHPEYDVRVTILGHIQRGGSPTASDRILASRMG
                     VAAVDALLDEQRSIMIGIVNDEIVHVPFTKAIKFDKPVNEDLLGVIQILSI"
     gene            complement(17040..17906)
                     /locus_tag="PLZ54_02295"
     CDS             complement(17040..17906)
                     /locus_tag="PLZ54_02295"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOJ65803.1"
                     /translation="MLNIEIDNKSGFCFGVVKAIEKAEEELEKGSTLYCLGDIVHNEE
                     EVERLQRLGLVTVNHEQYNQLHNVKVLIRAHGEPPSTYETARKNNITLIDATCPVVLK
                     LQSRIKKNYEELASEDGQIVIYGQTGHAEVKGLVGQTDGKAIVIEEEKDLDKIDFSKK
                     VHLFSQTTKDIEHYHHLIEEISKRMVKKDDFKFSDTICRQVANRIPNIINFAQQNDVI
                     LFVGGKQSSNGKVLYEHCKSINPQTYYISDCKQVNELEIQKDKKIGICGATSTPKWLM
                     DCIAKRLEERLA"
     gene            complement(17972..18658)
                     /gene="cmk"
                     /locus_tag="PLZ54_02300"
     CDS             complement(17972..18658)
                     /gene="cmk"
                     /locus_tag="PLZ54_02300"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HOJ65804.1"
                     /translation="MKKIVVAIDGFSSGGKSTMAKDLARDVGYTYIDSGAMYRAVALY
                     AMRNGWITENDIDIEALKKNIGSLKIDFKTNEHGVSETYLNGENVEKEIRTLEVANAA
                     SRVSALDFVRRELVRQQQQMGLQKGIVMDGRDIGTVVFPEAELKIFLTASPEIRAQRR
                     MEEMLARGEKVDFQKVLANIKERDERDLHRSESPLKKAPDAIVIDNSNLTRDEQRELL
                     KTLFYQKTLE"
     gene            complement(18698..19966)
                     /gene="purD"
                     /locus_tag="PLZ54_02305"
     CDS             complement(18698..19966)
                     /gene="purD"
                     /locus_tag="PLZ54_02305"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOJ65805.1"
                     /translation="MNILLLGSGGREHALAWKIAQSSQLTNLYIAPGNPGTSTLGTNL
                     PFSADDFQSIKIAVLEHEIDWVIVGSEDPLVKGIADFFASDKQLKNVSVIGPSKIAAQ
                     LEGSKNFAKKFMLRHGIPTAKYFSVTSETIDEGIQFLQQLSSPYVLKADGLAAGKGVL
                     IIDSLEEAEQELKSMLEGKFGTASKTVVIEQFLSGIECSVFVITDGNNYKILPEAKDY
                     KRIGEGDTGLNTGGMGAVSPVSFADKYFMKKVEEYIIRPTINGLREEGIVYKGFIFFG
                     LINVKGEPYVIEYNVRMGDPETEAVMLRIKSDFVDLLGGVVNENLDEKEIKIDERFAA
                     TVMLVSGGYPQAYEKGKEISGLDKVEGSIIFHAGTALKNGKLVTNGGRVMAISSYGNT
                     MKEALEISLRNAEKINFEGKYFRKDIGFDL"
     gene            20091..20450
                     /locus_tag="PLZ54_02310"
     CDS             20091..20450
                     /locus_tag="PLZ54_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ65806.1"
                     /translation="MSTYEQLDALARYILPKEILDDFDIVGIEEKSGVLNSIFLKDNN
                     KIKYLSNVAESQFKSFNTIAATVYEHHEEVLNFFINRSTNASAESFNAKIKSFRASLR
                     GVIDIKFFLFRLTNIYA"
     gene            20712..22895
                     /gene="recQ"
                     /locus_tag="PLZ54_02315"
     CDS             20712..22895
                     /gene="recQ"
                     /locus_tag="PLZ54_02315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOJ65807.1"
                     /translation="MLKKVSLAKELKKYFGFDTFKGNQEQIIQNVLNGNDTFVLMPTG
                     GGKSLCYQLPSLLMDGTAIVISPLIALMKNQVDAMRNFSEEDGVAHFINSSLSKQAIE
                     QVKCDILSGKTKLLYVAPESLTKEENIEFLKHIKISFYAIDEAHCISEWGHDFRPEYR
                     RIRPIVNEIGIAPIIALTATATPKVQHDIQKNLGMLDAKVFKSSFNRPNLYYEVRQKT
                     NNIDKDIIKFVRSQEGKSGIIYCLSRKKVEELAEMLHVNGISALPYHAGMDAAQRSEN
                     QDKFLMEEASVIVATIAFGMGIDKPDVRYVIHYDMPKSLEGYYQETGRSGRDGGEGQC
                     IAFYSYKDLEKLRKFMQGKPLAEQEIGNQLLIETQAYAESSICRRKLLLHYFGEEYKE
                     DNCGNCDNCMKPQKFMEAQELLTLVLETISILKEKFKAEHIVDILKGNETSDVVSYQH
                     NELENFGAASDEDEKLLQAVIRQALISGYITKDVETYGVLKITPKGKDYLKNPTSFPI
                     VKDNDFEEDEEVNLKNIASTSAADEVLFSILKDLRKKLSKKLDVPPFVIFQDPSLEAM
                     ATTYPITMDELQNIPGVGAGKAKRYGDEFLKVIKEYVKENDIIRPEDLRVRTVANKSK
                     LKVSIIQAIDRKIALDDIALSKGLDITELLDEIEAIVYSGTKINIDYFLEDVMDADRV
                     DEIFEYFNSAETDSIDDALDKLGIDDFSETEIRLVRIKFLSEVGN"
     gene            complement(22965..23837)
                     /locus_tag="PLZ54_02320"
     CDS             complement(22965..23837)
                     /locus_tag="PLZ54_02320"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOJ65808.1"
                     /translation="MKVAIFGNIYRPVIINSIKVLFDFFSEKQIPLMLDSDLYDFLVH
                     IGGYRLENVEKIENDNFKADVALSIGGDGTFLNTAARIGDKEIPIIGINTGRLGFLTD
                     VSNDEIIEALEALLEGKFRIEERTLLLVKTSDGTPLQYPYALNEVSVLKQDSSSMISI
                     NASVNGELIHTYQADGLLVSTPTGSTAYSMSVGGPIVVPQAQNFILSPVASHSLNVRP
                     LIIPDNWNIDLEVHSRTHSYLVALDGRSKVLEQTTQLHIAKAPFTIKVIKQLQHTFFD
                     TLKKKLMWGVDKRN"
     gene            24251..24964
                     /locus_tag="PLZ54_02325"
     CDS             24251..24964
                     /locus_tag="PLZ54_02325"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOJ65809.1"
                     /translation="MTKLSVNINKIATLRNARGGNTPDVCKAAIDCEKFGADGITVHP
                     RPDERHIRYSDVWALKPILQTEFNIEGYPSPNFMKLVTMVKPTQVTLVPDPPEAITSN
                     AGWDTEKHLDFLKDITAELQDAGIRVSIFVDANIKSVEYAALAGADRVELYTEPYARE
                     YAENREKAVATFIEAAKAAKKLGLGLNAGHDLNLQNLHYLHACIPWLDEVSIGHALIC
                     DALYLGLQETIKQYKNQLK"
     gene            25015..25707
                     /locus_tag="PLZ54_02330"
     CDS             25015..25707
                     /locus_tag="PLZ54_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOJ65810.1"
                     /translation="MINLLILQASANVATTVADPLQSEINLWTMAKFGGPIMIILALM
                     LAFAVYLFIERLITINQASKEDAFFMERIKDYIYEGKIETALKLCRDTNSPSARMIEK
                     GITRLGHPLNDVLVAIENVGNMEIAKLEKGLVIMASISGGAPMLGFLGTVTGMVSTFF
                     NLSQKAGGNILLGDLSVGMYQAMVTTIGGLIVGILAYFAYNYLVSRVDSVVNILESKT
                     MEFLDLLNEPVK"
     gene            25729..26145
                     /locus_tag="PLZ54_02335"
     CDS             25729..26145
                     /locus_tag="PLZ54_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOJ65811.1"
                     /translation="MALKKRNKVEAGFAMSSMTDIIFLLLLFFVIASTLSSPNDIKLN
                     LPESRSKSSTKQIIAKVAIDAEGKLSVAKGNQKPVPIEPKLLESYLLNMASQDSISFV
                     ALYADQNIAYKEIVRVLDIANQHGFKIVIATKPFEN"
     gene            26152..26844
                     /locus_tag="PLZ54_02340"
     CDS             26152..26844
                     /locus_tag="PLZ54_02340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOJ65812.1"
                     /translation="MKKSELYGIVGSIIFCMMLLVLLLIIKMHASTSVEPEGIMVSFG
                     ETLEGGSRAASPSAAEEYAVSKKASEKILTQKDPSVSLDKKTDNREQERELSEKKRLE
                     QEAISKANIMEGIFDKKISGNENVGDENLLKGNPAGRGTSNGNSWSLSGRNLVGNLIT
                     PRYEQNVEGEVTVNIRVDENGVVEGVSIGVPTNISDATILKAAMEAAKNTRFSPGKGK
                     ATGTITYHFRLK"
     gene            26923..27468
                     /locus_tag="PLZ54_02345"
     CDS             26923..27468
                     /locus_tag="PLZ54_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HOJ65813.1"
                     /translation="MKAMVFLADGFEEIEAIAPIDIMRRAGIEVTTISVSREKEVRGA
                     HGVLVEADRLFEEVSFSDADLLFLPGGMPGTKNLNAHDGLKQLLLKHANEGKKLAAIC
                     AAPSILGELGLLKGKEAVCFPGFEETLKGAIVSDKKVVQSGNIITGKAAGAAIEFALK
                     LVENLKGKSAAEQVGLSIFAF"
BASE COUNT         8771 a         5377 c         5126 g         8322 t
ORIGIN      
        1 atccagagcc cattacaatt cttctatcac ctggaacgga agaagctttt acccaggctt
       61 ccatcgtgaa atctgtcagt gtattactct taatcggggt tgttacgtaa tcatttgaac
      121 cattaaaact caacgccttt ccaaattttc catcaaccca tgtaggacta ttgtacaaag
      181 taccattatt cccattacct gacctatcat aagccgtcgt tccgctgcct tcatccaaat
      241 gccagcttcc ttttaccgga ctattgctat ttatcactct tacaaaagta ttggttgcat
      301 tgctggcaga ttgggcagaa gaattcccat aatgcataaa gatttcattt gttccagcag
      361 taggcacttt tacccagatg atggaagtac ctgaagcatc ccatttctcc atccagtaat
      421 ttaattcagt tccattcccg tcgtaaaaac gaatatcact gccatcactg ttggcgtgag
      481 cataagcgaa attggaagtt gttaattcaa cttttacctg ataatcagct ataggtgttg
      541 ccggattcaa agtaatcggt actcgataac tgaaattctc ttccgctgat ttcatccatg
      601 cttccagtgt catggcattc gaaacattta atgttgaaga gttggccaca gaaacatact
      661 tgtgtgtccc gttaaaattc aaagctttgc ctgctccctg agctgaaacc tgcaaaataa
      721 gggaaaaatt aaacaaaaac atggctagcg aaaatgcagg caaacaagag ccaaatgagt
      781 aaaaaaattt tttcataatt tatcaattgt ttaagattgg taatgtgtaa agttttctga
      841 ttcgtatttt gataacaaac aaaatagtaa gatacaaatc caactctttt aatagaggca
      901 attttcttca tttcgcttaa aaccatttca ataatcaaat attaactcta ttacgtatta
      961 aataacaaat atatgaataa ataatagaaa ttgtaccgat ttagtcaaat ttttacaata
     1021 aaataaaaca aaataattgt tttttatcaa tgcttcgaat gtgatagttt aaaccagaaa
     1081 tattagccaa ttcaaaggaa aagtcaaagt cgcagtgctc catagttaga gcttgtctca
     1141 attgtattta agcagattct aacatttgtt tcaaaatgat actccgttat atggtgttat
     1201 ctctttgttt gtaatggata tatcgagatt cacaaacaac tgttttgtat ttttaaacat
     1261 tgccatttcc tccaaaacta aaatcttttc cttgttgcag attttgagtg tagggataaa
     1321 tgtgaattta ttgcagtaca tatcgtaaaa atcgactaat gatagatttt ttaataactc
     1381 atttctaaaa ttatgaatgt tgtcaattat ttatcccgtt tgatatagaa aactttatga
     1441 cttatcattt aataaaatat gttattggaa aacattatga catgtcgatt tgtttgtata
     1501 aatatacatt tcgttcggga aatgcaaatg attatttaaa aatttaatta ttattggaca
     1561 aaaagtatta ttttgttctt tttttattac ttttgcaaaa caaaatattc aaatatgagt
     1621 aaaaatacag aaaatatgat ttttgatgaa gcaatgatcc aacaattcta cgagtccttt
     1681 cctgaaaaaa ttgaacagat aaaaaaccgg cttggcagac cattgacttt aacagaaaaa
     1741 atactctata ctcacctctc ggccgacgaa acattaaaaa attttgttcg tggcatcgat
     1801 tatgtcaatt tctcacccga cagagtagcc atgcaggacg ctacggctca aatggctctg
     1861 ttgcaactca tgaattcggg acgttcaaaa acggcagttc cggctacagt tcattgcgat
     1921 catttgatac aagctcacgt ttcggctgaa aatgatttgg ataccgcaat aaaaacgaat
     1981 agcgaagtgt acaatttttt acgggctgtt tccaacaatt tcggtatcgg tttttgggaa
     2041 cccggttcgg gaattatcca tcaagtaatt ttggaaaatt atgcatttcc cggcgggttg
     2101 atgataggta ccgattcgca tacaccgaat gccggaggat taaccatgtt ggctgtgggc
     2161 gtcggtggtg ctgatgctgt tgacgtcatg tcaggactgc cgtgggaatt gaaaatgcct
     2221 aaaataatcg gtgtgaaatt aacgggaaaa ctttccggct ggacatctcc gaaagatgtt
     2281 attcttaaac ttactggcat gttaaccgta aaaggtgcaa ccaatgccgt aatcgaatat
     2341 tttggagaag gaacggcttc actttcggct acgggaaaag ctactatttg caatatgggc
     2401 gcagaagttg gagcaaccag ctctattttt cctttcgata acaaatcggc tgtctatctc
     2461 gaagctacag gacgtaattc aattgctgaa actgcaaaga aattggccga acatttacag
     2521 gccgatccgg aagttgtaca acatcccgaa aaatattacg accgactgat tgaaattgat
     2581 ttaagcacat tggaacctca catcaacggg ccctttactc cggatgcagc ttatccgatt
     2641 tcaatttttg ggaaagtggt gagagaaaag aattatcctc aaaaattgga ggtcgggctt
     2701 atcggttcat gtaccaattc ttcctatgaa gatctttcac gagctgtttc ggtattgcgt
     2761 caggcacggg aaaaaaatct gaaagttcgg tcgcaaatga ttattaatcc cggatccgaa
     2821 aaaataagaa gtacagccga acgcgatggc atgttggatg aatttaaaag ttcaggagcc
     2881 acaattatgg caaatgcttg cggcccttgc attgggcaat ggaaacgcga catagatgta
     2941 gcggatcgtc caaattccat tatcacttct tttaacagaa attttgccaa acgaaacgat
     3001 ggcaatccca atacccatac ttttctttct tctcccgaaa tagtggcagc tctttctatt
     3061 gccggcgatt tgtgtttcaa tccgttgact gacgagctgg aaaacgaaaa aggagaaaaa
     3121 atcaaactgg atgaaccaca aggttttgaa cttcctctgt tgggatacga aataaaagaa
     3181 tcgggttata tagcgccttc ggaagaaaaa atggaaatca atatcgatcc caaatcagaa
     3241 agattacaac tattgcaacc ttttccggca tgggatggca aagatatgat tgaattgcca
     3301 ttgctgataa aagttaaagg caaatgtact accgatcata tttcaatggc aggtccgtgg
     3361 cttcgttttc ggggacattt ggacaatatt tccaacaatt tgctcatggg tgccgtcaat
     3421 gcttgtaatg ataaaaccaa tgccgtactc gatccggaga ctaaacaata taccacagtt
     3481 tcggctctcg cacgcaaata caaggcaaaa ggtacaggct ctgtggtagt tgctgaagaa
     3541 aactacggcg aaggttcgtc tcgtgaacat gcagcaatgg aaccccgcta tctgaatgtt
     3601 aaagccatca tcgccaaatc atttgcccga atacatgaaa caaacctgaa aaagcagggt
     3661 atgctggctt taacctttat cgacaagtcc gattacgata aaattcgcga agatgatcgc
     3721 attagcattc ttggactttc agacttcaaa ccgggaaaaa atttgttgat tcaacttcat
     3781 cattccgatg gaagtgtcga acaatttgaa gtggcacaca gtttcaataa aactcagatt
     3841 gaatggttta aagccggtag tgcactgaat gccatgagaa aatgaaactt tttgttccaa
     3901 aactcaagaa aaattatttt ccaaaagcac aaattaatat aagagaaact tatcaataaa
     3961 aaatagaggt aaaaaatgaa agaagaactt ttaatttaca aattatctga gactatcaaa
     4021 aaaaccagcc aaatagacaa agaattgttt accaaataca acgtaaaacg tggtttaaga
     4081 aacgaagatc atacgggagt actggtaggt ttgacccgca ttggggacgt agtaggctac
     4141 gaacgtaccg aagatggcca attaaaggca attccgggta aactttacta tcgcggaatt
     4201 gatgtagaag aattggttca tggtattcag tccgaaaatc gtcttggttt tgaagaaact
     4261 gcttttttgc tgttatcagg gtatctgcct accaaagacg aactggaaac tttcaagaaa
     4321 atactcaatg aagctatgcc tcttcaacat caggcaacca tgaacattat ggcactgaaa
     4381 ggacacgaca tcatgaatat tttggcgcgc tgcgttttgg aacaatatat ttacgatgaa
     4441 gatcccgaca acatatcacg cgacaatctt attcggcaat ccatcaatct ggtttccaaa
     4501 tttccaacta tcattgccta tgcttataac gttcttcgcc atcacgaaaa aggacgatcg
     4561 cttcatgtcc gccacccgga tgaaaatttt tcttttgctg aaaatttctt gttcatgata
     4621 aaaggaaaag gacaatatac acccctggaa attaaaattc tcgacctggc tttgattctt
     4681 catgccgaac atggcggagg aaacaattcc acttttagcg tacgtgtaac cagctctacc
     4741 gaaaccgaca cctattcttc cattgctgcc ggcattggtt cgttgaaagg gccgcttcac
     4801 ggaggtgcca acctgaaagt aatgggaatg atcaaagaaa tgagagaaaa cattaatgat
     4861 tggactgacg ctgatgaaat tgatcaatat ctgaaaaagt tattgaacaa ggaagcttac
     4921 gataaaaccg gactgattta cggaattgga cacgccgttt ataccatcag cgatccgcgt
     4981 gccatattgt tgaaagatat ggctcgcgaa cttgccaaag aaaaaggcct tgaaaaagaa
     5041 tttgcatttc aggaactgat agaacagcgt gccgtagagg tatttatgaa ttataaaaat
     5101 gggaacaaca acaagcaggt ttgtgtaaat gtcgacttct attctggatt tgtttacgaa
     5161 gccattggaa taccgaaaga agtttacacg cctatttttg caatggcccg cattgttggc
     5221 tggtgtgccc atcgcatcga agaactcaac tttaccagta aaagaattat tcgtcctgct
     5281 tataagaatg tcaacgaaat tcagcaattt attcctctcg aaaaacgtta aatgcaatag
     5341 ttgaaaggat tttttaaaca aaaatccttt cggcttttaa aaggaaaagt atgaaaaaaa
     5401 taaaagttga acaaccggta gttgaactgg atggagacga aatgacgcgc attatctggc
     5461 gaattatcaa agagcagctc atccttccct atctggatat tgatataaaa tattacgatt
     5521 taagcataca aaatcgcgat cttaccaacg atgaagttac aattgaggct gcccacgcca
     5581 taaaaaaata caatgtggga attaaatgtg ccaccataac acctgacgaa gcaagagtga
     5641 aggaatttgg attgaaaaaa atgtggaaat cgcccaacgg aacaatcaga aatataatag
     5701 gtggaactgt ttttcgcgaa cctatcattt gttccaacat tcccagactg gtgcctaatt
     5761 ggacaaaacc catagtgatt ggccgtcatg cttttggcga tcagtacaag gcaaccgatg
     5821 tacccatcaa aggaaaggga acactaaaaa tgacttttac tgccgaaaac ggagaaacac
     5881 aggagtggga agtttataat tttcaaggag atggagtagc catgagtatg tacaatactg
     5941 atgaatcaat ttatggattt gcccgctcgt cctttcaggc agcactcgat aaaaaatggc
     6001 ccttgtattt gtccacaaaa aacaccattc ttaaaactta tgatggccgt tttaaggata
     6061 tttttcagga agtttacgaa aaagaattca gggaagcttt tcaaaatgcc ggcatcactt
     6121 acgaacatcg tctgattgac gatatggtag cttctgttct caaatggaat ggcggctttg
     6181 tatgggcttg caaaaactac gacggcgatg ttcaatccga ttctattgct caaggatttg
     6241 gttcactggg actgatgtcg tcggttctga ttacgcccga cggaaaaacc attgaggcag
     6301 aagccgcaca tggaacagtt acacgtcatt acagacttta tcagcaagga aaagaaactt
     6361 ctaccaatcc tattgcttcc atttttgctt ggacacgcgg actggcacat cgcggaaaac
     6421 tggatggcaa taatgcacta atcgatttcg caaaaaaact tgaagaagtc tgtgtacaaa
     6481 ctgtagaatc gggaaaaatg acaaaagatc ttgctatact tgttcatgga gaaaaagtta
     6541 gccgaaccga ttatctgagt accgaagaat ttctgacagc attgaaacaa aatttggaag
     6601 caaaactttc cgcttgattc aataattttc ttaaacaata aaactaaaga aagctgcttg
     6661 aaaaaatcaa acagcttttt ttacattatg aatcagaatt accgaatgtt attcgataga
     6721 tttctctgaa gtttcagctt cttcagaagc aggtgtttct tctgcttcaa caaccttttc
     6781 ttcaaccttt tcggtttttt ccttcttact cgatttctga ggtttttctg acttttcttc
     6841 cgtttcggca ggtttttcag aaggttcttc cacttttgtt tcctctgcca tctttacttc
     6901 tgcaggttct tctttttcag ctggctcagc ttttttggat tttgtttcct ttttggccaa
     6961 ttttttagcg gcttcacttt ccatctgatc tttcaaatct accaatgact gaatatctcc
     7021 aaaagtagtt ttttcgatag cctgagtctg cacggttgaa gattcttctt tctttgtacg
     7081 tttggtaacc gatttctttg gagaagtcga agtttcacct tctgcacttt cagttttggc
     7141 agcacgtttc aggtcttcgt gaatacgtgt gtgggaaagt acaattttct ttgcatcctt
     7201 gttgaattcg ataactttga aaggaagttt ttcgtcaacc tgtgcctgtg taccgtcttc
     7261 ctttaccaac tgtttggctg aagcaaatcc ttctacacca taaggcaaag caataacagc
     7321 acctttatcg agcatttcaa caactacgcc gtcgtgaata ctatccacag caaatacgct
     7381 ttcgagcgca tcccacggat tttcttccaa ctgtttgtgt cccaaactca aacgacgatt
     7441 ttccttgtca atatcgagta ctactacttc gatttcactt ccaatttgcg taaattcaga
     7501 aggatgtttg attttctttg tccacgacaa atcggaaata tgaatcaagc catctacgcc
     7561 ttcttcaatc tctacaaata cgccgaaatt ggtaaaattg cgaactttgg caacatgttt
     7621 gctcccaacc ggataacgtt cttctatatt ttcccaagga tcggatttca gctgcttgat
     7681 gcccaacgac attttacgtt catcgcggtc gagcgtcaaa ataatggctt ccacttcgtc
     7741 gccaactttc aaaaagtctt gtgccgaacg aagatgttgc gaccaactca tttctgatac
     7801 gtgaatcaat ccttccactc ctggagcaat ttctacaaat gcaccataat cggtaatcac
     7861 aaccactttg ccttttacct tgtcgcctac ttttaaattg ggatccaacg catcccaagg
     7921 atgtggagta agctgtttca aacccaaagc aatacgtttt ttctcttcat caaagtccag
     7981 aataacgacg ttgatttttt cgtccagcga aacaacttca cttggatgat tgatacgtcc
     8041 ccacgacaaa tcggtaatgt gaatcaagcc gtccacgcct cccaagtcga taaatacgcc
     8101 ataggaagtg atattcttga caacaccttc gagaatttgt cctttctgca atttgctgat
     8161 aatttctttt ttctgttgct ccaattcttc ttctatcaga gctttgtgcg atacgacaac
     8221 atttcggaat tcctgattga tttttacaac tttgaattcc atagttttgt tgacgaaagc
     8281 atcgtaatcg cgaataggtt taacatcgat ttgtgaaccg ggcaaaaatg cttcgatacc
     8341 aaacacatcg acaatcatac cgcctttggt acggcattta atgaaacctt taactacttc
     8401 ctgcgattcg agagctgcat ttacgcgatc ccatgcccga ctaatacgtg cctgcttgtg
     8461 tgaaagcaat aattggcctt tcttgtcttc ctgactttca atataaactt caaccttatc
     8521 gccgactttc agatcgggat tgtatcgaaa ttcgctcatg ggaacaacgc catcggattt
     8581 ataaccgata tttacaacaa cttcacgttt gttgatggaa atcaccgttc cttctaccac
     8641 ttctttgtcc ttaacggcat tcagggtttt gtcataaaca ttttccaatt ccttttttgc
     8701 ttcagtagtc agggtatttc ctttttcata agcatcccag tcaaattctt caggggttat
     8761 ttcagcttga actggggcaa ctgattcctt tacaggttct tcaggtacag aaacttcggg
     8821 tgtttctgca acaattggtt ctgctgcatg cgattctgca acactttctt cttcgggaga
     8881 cagaacttca tttacctctt gagtttcttc ttttttggct tcaacagctt ctggagaaac
     8941 attttctgtc agggcagatt cgttcttttc taaattgatt tggttttctt ccattctaaa
     9001 aatttgtttt tttactaata aatagtagat tagactttat gttgacttaa aaaaatacgg
     9061 gcacaaagat acaaaaagtt tatgatttga tttataattt tttacagaaa cttatttatt
     9121 acaggggcat tttttgaagt tctgtttcaa tgctttttga atagcggctt cagcaagtgg
     9181 ttccatcacc tgatagcctt tttgattggg atgaactcca tcttcggaaa attcggattt
     9241 taaaccgttt ctttcatctg ccattgccga gaaataatcc agataaacaa atccgttttt
     9301 ctgacaataa tctttaatcc atgcgttcag agttacaatt ttctcagccg gctgcaaacc
     9361 ccgacgccaa ggataatcat aggcaggcaa tacggaggaa agaaccacct taatattgtg
     9421 aacccgtgcc aattctgaca tagaagaaag attgtcttca atcatttcga gtgttgaagg
     9481 ccctgtattg cctgcaatat cgttggtgcc ggctaaaatt actactaccg ccggttgcaa
     9541 tttaattaca tcctgacgaa agcgcaaaag catttgaggg gttgtctgac cgctgatacc
     9601 ccgattgata taggaacgat ttttaaaaaa atcgggacga atattaatcc aaccttcggt
     9661 gatggaattg cccaagaaaa ctactctttt ttcaccttta tcaggggctc ccaatttttc
     9721 attttctgct ctgaaacgac ctaaatttgg ccagtcttgc gcattcaatt gctgcatgta
     9781 aaaacacaac atagtcataa ttaaaatatt aacaaaatac tttgacttca taacgataaa
     9841 tatttaagta aatggattgt aaaaaatatt ttggttatca aattttacga acaacaactg
     9901 tcgtaatttt atgatttaca aaagtagtta aaaatagata taaattttct acgattactc
     9961 tgaaaagtac aacataacga ttcgatcgat ttgcattaaa aaaagatttt tttatcacaa
    10021 agaatttagt tcttcataag aattgtggat tttaccaaca aaattttcta catttgcaat
    10081 gttcacagtc ttcaacatta tttcgataaa acgacatgaa aacacataca aaaaaatttt
    10141 atttcccgaa ttttgagaaa ataatctgtt tattcctact attgactgga tttttctcaa
    10201 aaactctttc gcaggacttt gccaaaggag ctgatgtaag ctgggtaaca caaatggaag
    10261 ctgccggaaa aaaattttat actgccgaag gagtggaaac ggaatgtatg acgttactga
    10321 aaagtttggg aatgaatacc atccgtctcc gtgtatgggt caatcctgcc gatggatggt
    10381 gcaacgctca ggatgtactt ttgaaagcac aacgagcaaa cgatttgggc atgcgcatta
    10441 tgattgattt tcattatagt gactcttggg ccgaccccgg gaaacaaacc aaacctgcag
    10501 catggtccac tctttctttt gccgacctga aaacagctct ttcccaacac actaacgaag
    10561 tacttacttt gctgaaaaac aatgatataa ctccggaatg ggttcaggtt ggcaatgaaa
    10621 cttctgatgg aatgctttgg gaagatggaa gaatttccaa aaatccacaa aactacgccg
    10681 agttaaacaa tgcaggatat gatgccgtaa aatccgtttt ccccaatgcc aaagttatcg
    10741 tccacttacc caatggctat agcaacagtt tgtaccgttg gtttttcgac acgttaaaga
    10801 attatggcgg gaaatgggac gttatcggca tgtcggttta tcccttttgg tacacgacca
    10861 aaaacgactg gagaaattgc aatatcagtt gtttcaacaa tatgaacgac atggtaagtc
    10921 gatacggtac agaagtgatg atagtggaat gcggtatgca atgggatgct ccggaagcat
    10981 gcagagattt tctttccgat ttgattaaca aaaccaaaaa tgtttcgcaa ggaaaaggat
    11041 tgggcgtatt atattgggag ccgcagtgtt atcccggatg gaacggatat acattgggcg
    11101 ctttcgacaa tacaggaaaa cctactattg ccctcgatgc ttttaaagaa gtcagtcaat
    11161 cgacccatcc gctccaacta aaaaccggat ggcgttatga tgacttgcaa aaagtcatac
    11221 ttttcgataa aacattcagt aaagttactt tatttgacag cgccggcact gccatacaaa
    11281 gtttcgcttc taccaatcgc atttctatga aaaacatgca aaaaggaatt tatatccttc
    11341 atgcagcatt gaaaaacacc gaaccacttt taatcaaatt catttacaat taaatgctgc
    11401 tgattattct ttgctgaaac aatatccatc taaacttttt aaacgttttt tccaacttat
    11461 attattttgt tcaattctaa aaacagaaat cggttctttt gaaatacatt gaagtcatat
    11521 tgcctttgcc attgcccaat acttttacct atgctgttcc cgaagcagat gagaactcga
    11581 tacaacaagg tatgcgagtt gtagtaccat ttggtaaaaa aaaattatat tccggaattg
    11641 tatattatgt tcatattctt cgtccggaaa cgacttatca aataaaagaa attgtcggta
    11701 ttttggatga ggaacccatt ttgcgccgac ctcaaatcaa attttgggaa tggatagcca
    11761 attattatca ggcacatttg ggggaagttt atcaatcggc tttaccggcc ggtcttaaac
    11821 tggaaagtga aactcttgta cgggtgaagc cggattttga taccaacgtc catttttcag
    11881 aacgcgaaaa aaaaattctg gatgtcattt taaatcagaa aaagcccatt tcattgtcag
    11941 cgttggcaaa agatagcgga atttcaaacg ttttacctca actaaaatta ctgctggaaa
    12001 aaggcgtcat cgaaatcagc gaacagctta ccgaaaaatt tcatcttaaa actgaaacat
    12061 acatacgtct tcgtcctgaa attcaacagg atgaaaaact tcacgaaatt tttgaccaac
    12121 tgaaatcggc aaaaaaacag ttaagtttgc tgatgaacta tattgatctt tcaaagtgtc
    12181 ttgatccaaa aaatgtccgg gaagtttccc gcaaaacact tcttgaaaaa tcaggcattt
    12241 ctcccgctgt tctgaaagcg ctcaccgata aaaagatttt agaaacctac gagaaaattg
    12301 tcggacgtct cgacttttct gaaaacgaac ttcagaaacc ctctattctt aacgagtttc
    12361 aaacaaaagc actgcaagaa atcgaaaaaa aatttaaaga aaaaccggtg gtattgctgc
    12421 atggcgttac ttcgagcggg aaaacggaac tttatattca tttgattcaa aaagttttaa
    12481 acgaagggaa acaggcactt tacctggtgc ccgaaattgc actaaccacc caactcactg
    12541 cccgactgaa aagaattttc ggtaaaaagc tgggagttta ccattcaaaa ttttccgacg
    12601 cagaacgcac cgaaatttgg aacaatttgc tgaacgacaa aggttatgaa gttattatag
    12661 gcgttcgttc ctccattttt ttgcctttcc gacaattagg tctggtaatc gttgacgaag
    12721 aatacgaaac aagttacaaa caatttgacc cggctcctcg ctatcacgca cgtaacgctg
    12781 ccattgtact ggcttccatg cacggagcac acactttgct cggttcggca acaccggcaa
    12841 tagaatccta tttcaacgct gttacgggca aatacggact cgtaaaactt gatcggcgat
    12901 ttgaagaaat ttcgttgccc gaaatcgtgg ttgtagattt gaaagaagct tatcacaaaa
    12961 aacaaatgaa cggccatttt tccgatttgc tgatgcatga aatgcagaaa actcttcaaa
    13021 acaaagaaca aataattctt tttcaaaatc gacgggggta tgcgccctat ctggaatgta
    13081 aaagctgcaa ctacataccc aaatgcaaaa attgcgatgt cagtctaact tatcataaaa
    13141 ctaccaatgc tctgacctgc cattattgcg gatacactga atcttatcct gcttcctgtc
    13201 cggtctgcgg aacgccggcg gctttctcca cgcacggatt cggaacagaa aaaattgaag
    13261 atgaaataaa aaaaatcttt cccgaagtcc gaattagccg aatggatctg gataccaccc
    13321 gttcaaggaa atcgtatgaa acgattatca ccgattttga agagtataaa atcgatattt
    13381 tgattggcac tcaaatgatt accaaagggc tggatttcga caaagttagt ttggtaggaa
    13441 ttctaaacgc agataatatt ctgaattttc ccgattttcg atcacacgaa cgagcttttc
    13501 aattgctatc acaagttagc ggtcgtgccg gcagaaagga taaacaggga aaggtgattc
    13561 tgcaaaccta tcaaccgaaa cacccaatta ttcaacaagt aatgaccaac gactacgaag
    13621 gaatgtttgc aaaccagtgc gaagagcgca aacagttcaa atatccaccc tatacacgac
    13681 tgattttact tatagttcgt catcacgaca gacagatact tcaggaagct tctcatcaac
    13741 tgaccaactc gcttcggagc gtttttggcc gcagggtaca gggtcccagt gatccgctaa
    13801 ttcctaaaat tcaaaacttt tatattaaac atattctatt aaaaatagaa atagaagcat
    13861 ctcctgaaaa aacaaaaaaa ttgatacgag attgcatcaa tctttccctc tcgcaacctt
    13921 cattcaaatc ggttcattta caaatagatg tagatccact ctaaactcca accaaatctg
    13981 attagataaa agtatttccc ctctttcatt ttatttaaaa tagatcatta attttggaat
    14041 tcgtaaaatg aattgattta aagaattcta tctcgatata taaaataaga atttaacctt
    14101 agtcccaatt caaaaaaacg ataaacaatc aaacaaaatt aattggataa ataaaaaaag
    14161 agcaatgtaa ataacttgga ttttctgaat tttatatttt cattttggca aatgataaaa
    14221 taataaacaa gttaacctat aagtataaag ttaaactttt caaaaaattt tatattaatt
    14281 aattgattat aaacatatta gtattatata ttaaagtgtt gatcaagaaa tgattttttg
    14341 ttcacctaat attgccatat aaaaaataag tattaatttt gcacacattt agctaaaaca
    14401 tcaattttaa ctgaatcgga taatacattt tattgcaatt taagtaagtt atatttatag
    14461 aaaatcaaga aaacaagcga tgaaaaacaa gtatattgat ctgattgaac agacttttga
    14521 attccccaat gatgaattca atgtcattga caatgaactc tggtggtgtg gtgttccact
    14581 gatggatgtc atcaagcagt atggaactcc gttgagaata gcctacctgc cgaaaatagc
    14641 cgagaatata caaaaagccc gacgtatgtt taatgtggct atggcaaaag ttgattatca
    14701 gggagattat atgtactgtt attgtactaa aagttctcat ttctcttttg taatggaaga
    14761 agttttgaaa catgacgtac atctggaaac ttcttccgct ttcgatatca acatcatgga
    14821 atcgctttat gaacaaggat tactgaagcc ggaaacatat attatctgca acgggtttaa
    14881 acgtccacaa tatatagaaa atattcaaaa tctgatacgt ttgggatttt ctaatgtagt
    14941 accgatattg gacaataaat ttgaattgga tttgctgctg aaagatttcg ataagaaatt
    15001 aaaaatagga atcagaattg ctgcggaaga agaaccgaaa tttgattttt atacttccag
    15061 acttggtatt cgatacaacg acattattcc ctactacaca aagcacatag ccaacaaccc
    15121 gcaggtcgaa ctgaaaattt tgcatttttt catcaatacg ggagtcaggg atacggcata
    15181 ctactggaat gaactgagta aagccgtaaa tttgtactgt gaactcaaga aaaaatgtcc
    15241 tgaactcgac agtttaaata tcggtggagg tttcccgatt cagacccact tggatatgtc
    15301 gtacgattac gaatacatgg ccgaagaaat catagcacag ataaaaaaca tctgcactca
    15361 gaacggagtt cccgaaccaa atatatttac cgaatttggt tcctacaccg ttggcgaaag
    15421 tggagccatg ctttattcca ttgtcaatca gaaaaaacaa aacgaccgcg aattgtggga
    15481 catgatcgac agttcgttta tgactacact gccagataca tgggcaatca atcagcggta
    15541 tgtatttttg gctatcaaca attgggattc agaatacgaa cgcgtcaatc ttggtggcct
    15601 gacctgcgac agtgaagact tttacaatgc cgaagttcat tcaaatgcta ttttccttcc
    15661 gaaaatggaa gaagggcggg aacaatatat tggatttttc catactggcg cttatcaaga
    15721 atcgttgagc ggatatggcg gcatacagca ttgtttgata ccggctccaa aaatggtaat
    15781 tatcgacaag gatgaagacg gagagtattt taccaaactt tttgccaaag aacaaagcta
    15841 caagtcaatg ttgaaaattt tgggttactg agtttaaaaa aatgttaaat aaaaaaccac
    15901 tgactagata aaatatcgca ggcagtggtt tttaattttt cagggaacta caatgtttgg
    15961 taaaagaaaa aacaaaaaaa cattcatcaa taaatttagc gaatgaaaat tgttaaattg
    16021 acaggatttg gatgacgccc aataaatctt catttaccgg cttgtcgaat ttaatggctt
    16081 ttgtaaacgg tacatgaaca atttcatcgt tcacgatgcc aatcataata ctgcgctgct
    16141 catccagcaa agcatctacg gctgccacac ccatgcggct ggccaaaatt ctgtccgaag
    16201 cagtaggtga accaccccgc tgaatatgtc ccaaaattgt tacgcgtaca tcatattcgg
    16261 gatgttcgcg gcgcattctt tcagccaagc cattggctcc gccggtaatt tcactttcgg
    16321 caacaatgac aatgctgctg tttttcgatt ttctgaagcc gtttttaatc agttcttcca
    16381 actgatcgac tttggtttcc ctttctggaa taatggcagc ctccgcacct gaagccaggg
    16441 cactgcttaa agccagaaaa cctgcatcgc gtcccatgac ttctataaag aataaacgtt
    16501 cgtgggaaga agccgtatcg cggattttgt ccacagcatc gataatggta ttcaaagctg
    16561 tatcataacc tatagtaaca tccgttcccc ataaatcgtt gtctattgtc cccggcaagc
    16621 ctacaatggg tacgttaaat tcttgtgcaa aaattcgagc accggttaaa gttccatcac
    16681 ctccgataac aatcaacgca tcaatttctt ctttcctcat agtttcgtaa gccatttttc
    16741 ttccttcggg tgtcataaat tcagcgcacc tggccgtttt aataatcgtg cctccttgct
    16801 gaataatgtt gctcacgttt tcggtcttga attctttgat ttctccggtt atcagtccct
    16861 tgtaacctcg gtaaatcgct tttacacgaa taccgttgaa aattgccgta cgtgttactg
    16921 ccctgatagc agcattcatt ccgggagcat cacctccgga agtaagtaat ccgatacatt
    16981 taatttcgta tttcatttct attttgttga ttaattgatt atcaaaaaac taataaaaat
    17041 caggctaacc tttcttccaa acgtttggca atgcaatcca tcaaccattt tggtgtggaa
    17101 gtagccccac aaattccaat ttttttatcc ttttgaatct cgagttcatt cacttgttta
    17161 caatcggaaa tataatatgt ctgtggatta atgcttttgc aatgttcata aagaactttt
    17221 ccgtttgagc tttgcttgcc acctacaaaa agaataacgt cgttctgttg tgcaaaatta
    17281 ataatatttg gtattctgtt tgctacttga cggcaaatgg tgtcgctaaa tttaaaatca
    17341 tcctttttca ccatgcgttt tgaaatttcc tctatcagat gatgataatg ttcaatatct
    17401 ttggttgtct gagaaaacaa atgtactttc tttgagaaat cgattttatc caaatctttt
    17461 tcttcctcta tcacaatggc tttaccatcg gtttgcccta ccagtccttt gacttcagcg
    17521 tgtcctgttt gtccgtaaat aactatttgt ccgtcttccg aagccaattc ttcatagttt
    17581 ttcttgatgc gcgattgcaa tttcagtacc accggacagg tagcatcaat cagggtaata
    17641 ttattctttc tggctgtttc gtatgtgctt ggaggctctc cgtgagcgcg aatcagcact
    17701 ttcacattat gcagttggtt gtattgctca tgattgacag taacgagtcc caatcgctga
    17761 agtcgttcca cttcttcttc gttgtgcaca atatctccca aacagtatag agttgagcct
    17821 ttttccaatt cttcttctgc tttttcaatg gctttaacca cgccaaagca aaatccggat
    17881 ttgttgtcta tttcgatgtt caaaattttg aagctttata aaaattaaaa aaacagatga
    17941 ataacactga aactttaatt gaatcagctt attattctaa cgttttttga taaaataaag
    18001 ttttgagcaa ctctcgctgt tcgtcacgag tcagattgga attgtctata acaatggcat
    18061 cgggggcctt tttgagtggg ctttctgaac gatgaagatc tctttcgtcc cgctctttta
    18121 tattggcaag cactttttga aaatcaactt tttcacctct ggccagcatt tcttccattc
    18181 ttcgttgagc tcggatttcg ggagaggcgg ttaaaaaaat cttcagctcg gcttcaggaa
    18241 aaactacagt tccaatatct cttccatcca ttacaattcc tttttgcaaa cccatttgtt
    18301 gctgctgccg caccagttct ctccttacaa agtccaaagc acttactcgg ctggctgcat
    18361 tggcaacttc caatgttctg atttcttttt cgacattttc gccattgaga taagtttcgg
    18421 aaactccatg ctcattggtt ttaaaatcga tttttaaaga gccgatattt tttttcagtg
    18481 cttcgatatc aatatcattt tctgttatcc aaccattgcg catggcatac aaggcaactg
    18541 cacgatacat ggctcccgaa tcaatgtaag tatatcctac atctcgtgca agatcttttg
    18601 ccatagtact tttaccgccg gaagaaaacc catcaatagc gacaacaatt tttttcatcg
    18661 ggattaattt ttatttgcag caacaaattc caagctttta taaatcgaat ccgatatctt
    18721 ttctgaaata ttttccttca aaattaattt tttcggcatt tctcaatgat atttccagcg
    18781 cttctttcat ggtgtttcca taagaactaa tggccattac tctgccgcca ttggttacca
    18841 gtttcccgtt tttcaaggca gtgccggcat gaaaaataat acttccttcc actttatcca
    18901 aaccggaaat ttccttgccc ttttcataag cttgaggata gccgccggaa accagcatta
    18961 ctgtggctgc gaaacgctcg tcaattttaa tttctttttc gtccaaattt tcatttacca
    19021 cacctcccag taaatcaaca aaatcggatt ttattcgcaa catgacggct tctgtttcag
    19081 gatcgcccat ccgaacatta tattcgatca cgtaaggttc acctttaaca tttatcaaac
    19141 caaaaaaaat aaacccttta tatacaattc cttcttctct caatccgttg atagtgggtc
    19201 gaataatata ttcttctact ttcttcatga aatatttatc ggcgaacgaa accggcgaaa
    19261 cagcgcccat tcctccggta tttaatccgg tatcgccttc acctattcgc ttgtaatctt
    19321 tggcttcggg cagaattttg taattatttc catccgttat cacaaatacc gaacattcaa
    19381 ttccggaaag aaattgctca attacaacgg ttttacttgc tgttccaaat tttccttcca
    19441 gcatggattt taattcctgt tcagcttctt ccaagctatc gataatcagc acgcctttcc
    19501 ctgcagccag tccgtcggct ttcagtacgt aaggagagga aagttgctgg agaaattgta
    19561 tgccttcgtc aatagtttct gaggtaacgc tgaaatattt ggctgtcggg atgccatgtc
    19621 tgagcatgaa ttttttggcg aaatttttac tgccttccag ttgcgcagca atttttgacg
    19681 gcccgatcac agatacgttt tttaattgtt tgtcagaggc aaaaaaatcg gcaatccctt
    19741 tcaccaaagg atcttccgac ccaacaatta cccaatcgat ttcatgttcc aacactgcta
    19801 ttttgatgga ttgaaaatca tctgccgaaa aggggagatt ggttcccagc gttgacgtgc
    19861 cgggatttcc gggagcaata taaagatttg taagctgtga actttgagca attttccatg
    19921 ccaaagcatg ttcacgacct cctgaaccca gaagtaatat attcatgttg tagagttgtt
    19981 tttacgttgt gcaaaagtaa catttttcat attttcgggc aaacaaaaat ggctcacctg
    20041 tataaatttg tagaaaaaga aaagtatctt tgtgaaaaaa tactttttat atgtccactt
    20101 acgaacagct tgatgcactt gcccgttaca ttcttccgaa agagatatta gacgattttg
    20161 atattgttgg aattgaagaa aaatcgggag ttctcaattc aatttttctt aaagacaaca
    20221 acaagataaa gtatctctca aatgtggctg aaagccaatt caagtctttt aatacaatag
    20281 ctgctacagt ttatgaacat cacgaagaag ttcttaattt ctttatcaat agaagtacca
    20341 atgcctctgc cgaaagtttc aatgctaaaa ttaaatcctt cagagcttct ttgagaggag
    20401 ttatcgatat taaattcttt ctcttcagat tgactaatat ctatgcttaa acacatattt
    20461 ttacgggtga gccctatcct cccgtcaaag ttgcatttac taattgaatt taagttgtat
    20521 atatcctgta ttttttttga aaaaaacttg taggtttaaa aagagtttat aattttgctt
    20581 catgattaag ctattaattc cttttctaca aatccaagaa aataattcaa aaaaattcag
    20641 ggaaacagtt ctgataattc atcaacattt actaatttcg tgcttgtttt ttttgacaaa
    20701 ctattttttt catgttaaaa aaagtctcat tagctaagga gctaaaaaaa tattttggtt
    20761 ttgatacctt caaggggaat caggaacaaa ttattcaaaa tgtgctcaat ggaaacgaca
    20821 ctttcgtcct tatgcctaca ggaggaggta aatcattgtg ttatcagctt ccttctcttt
    20881 taatggatgg aacggccatt gtcatttctc ctctgattgc tctgatgaaa aatcaagtgg
    20941 atgccatgcg caatttcagt gaagaagatg gcgtggctca ctttatcaat tcttcgttgt
    21001 cgaaacaagc tattgagcaa gtaaaatgtg acatcctgag cggaaaaact aaattgctat
    21061 atgttgctcc cgaatcactc acaaaggaag aaaatatcga atttctcaaa catataaaaa
    21121 tttcctttta cgctattgat gaagcacact gtatttcgga atggggacac gattttcgtc
    21181 ccgaatatcg acgtattcgt cctattgtca atgaaatagg aattgctccc atcatagctc
    21241 ttactgccac tgccactcca aaagtgcaac acgatattca aaagaatctg ggaatgttgg
    21301 atgctaaggt tttcaaatct tctttcaacc gtcccaatct gtattatgaa gttcgtcaaa
    21361 aaacgaacaa catcgacaag gatattatta aatttgtacg ttctcaagaa ggaaaatcag
    21421 gcataatcta ttgcctgagc agaaaaaaag tggaagaact ggccgaaatg ttgcatgtaa
    21481 acggaatttc ggctttaccc tatcatgccg gcatggatgc tgctcagcgg agcgaaaacc
    21541 aggacaagtt tttaatggaa gaagcaagcg tcatagtggc aactatcgct tttggcatgg
    21601 gaattgataa acccgacgtc agatatgtga tacactacga catgcccaaa agtctggaag
    21661 gatattatca ggaaaccgga cgcagcggac gtgatggagg cgaaggtcag tgcatagctt
    21721 tttattctta taaggatctg gaaaaactgc gaaaatttat gcagggaaaa ccacttgccg
    21781 aacaggaaat tgggaatcaa ctcctaatag aaacacaggc ttatgccgaa tcttccatct
    21841 gccgacgaaa acttttactt cactatttcg gtgaagaata caaagaagat aattgtggta
    21901 attgtgataa ttgtatgaaa ccccaaaaat ttatggaagc gcaagaattg ttgactttgg
    21961 ttcttgaaac catttctatt ttaaaagaaa aattcaaagc cgaacacatt gtggacattc
    22021 ttaagggaaa tgaaacatct gacgtagttt cctatcaaca caatgaattg gaaaacttcg
    22081 gagccgcctc cgatgaagat gaaaaattgc ttcaggctgt tattcgacaa gcccttatca
    22141 gcggatatat aacaaaggat gtggaaactt atggagtgct caaaatcacc cccaaaggaa
    22201 aagattattt gaaaaatcct acttcgttcc caattgttaa agataatgac tttgaggaag
    22261 atgaggaagt gaatctgaaa aatattgcca gcaccagcgc tgccgatgaa gtattgtttt
    22321 ccattttaaa ggatttacgc aaaaaacttt caaaaaaatt ggatgtaccg ccttttgtca
    22381 tttttcagga tccttcgctg gaagctatgg ccactacata ccctataaca atggatgaac
    22441 tacaaaacat accgggggtt ggggcaggaa aagccaagcg atacggagat gaatttttaa
    22501 aagtcatcaa agaatatgtg aaggaaaacg acataattcg tcctgaagat ttgagagtac
    22561 gtactgtagc caataaatcg aaacttaaag tctctattat tcaggctatt gacagaaaaa
    22621 ttgccctcga cgatattgcc ttgtccaaag gattggatat tactgaactg ctggatgaaa
    22681 ttgaggcaat tgtatattca ggtacaaaaa ttaatatcga ttattttttg gaagatgtta
    22741 tggacgcgga ccgcgtagat gaaattttcg aatactttaa ttctgcagaa accgacagca
    22801 tagacgatgc attggataag cttggaatag acgattttag tgaaacggaa atacggcttg
    22861 taagaatcaa gtttctctcc gaagttggta actaaacctt aaaaaaagga aagtttttca
    22921 gaaacaaagt aaaaagagaa ataatcctgt ttttttcgta tttgctaatt cctcttgtcg
    22981 acaccccaca ttaacttttt cttcaacgta tcgaaaaaag tatgctgaag ctgttttata
    23041 acttttatgg taaaaggagc tttggcaatg tgcaattgtg tagtttgttc caacactttc
    23101 gatcgcccgt cgagcgcgac aagatagctg tgtgtacgac tgtgaacctc aagatcgatg
    23161 ttccagttgt cgggaattat caaaggcctc acattcaaac tatgagaggc aacaggcgaa
    23221 agaataaaat tttgtgcctg aggtacaaca attgggcctc caacactcat agaataagca
    23281 gttgagcctg ttggcgttga aaccaataaa ccatcagcct gataggtatg aatcaattcg
    23341 ccgttgacgc tggcattgat gctgatcatg gaagaagaat cctgtttcaa aaccgacacc
    23401 tcgttcaaag cataaggata ttgcaacggc gttccgtccg aagttttcac caaaagtaaa
    23461 gtacgttctt ctattctgaa ttttccttct aaaagagctt ccaacgcttc aataatttca
    23521 tcattcgaaa catcggtaag aaaacccaac cgtccggtat tgatgccgat aatgggaatt
    23581 tctttatcac cgattcttgc cgccgtattt aaaaaagttc catcacctcc aatactcaaa
    23641 gcaacatcgg ctttgaaatt gtcattctcg attttttcca cgttttccaa ccgataaccg
    23701 cctatatgta caaggaaatc gtacaaatca ctgtcaagca ttaaaggaat ttgtttttcc
    23761 gaaaaaaaat cgaacagcac tttaatactg ttaatgatga ccggcctata tatatttcca
    23821 aaaattgcaa ctttcatcag aatataattt attttttaat ggtctgatgg aacccacatg
    23881 tctgaatatt atattcattc gtgtttgtat atgtaataca tgtgggtttc atatcaagaa
    23941 ttttttttac aaaaaaagaa gaaacattct gtaaaacaag gaattgaatt aataaaacgc
    24001 atagtttttt attaaaaaac aaaaacatag acaattcttc aagtgcgact tcaaaatttt
    24061 gagttatttt tgtagagaaa atgaatctga aactttacac aaaggaacta aatgaaaacg
    24121 acatttacaa ataaatattt ttctaaacaa aatgattttt tctctttttt tcatattgtg
    24181 gaacggagat actattgttt gtttaatttt atttaaccat caagaaatga ataattaaaa
    24241 ttcaattgac atgacaaaat taagtgtaaa tataaataaa atagctacct tgcgcaatgc
    24301 acgaggtggc aatactcccg acgtctgcaa ggccgccatc gattgtgaaa aatttggagc
    24361 cgatggcata accgttcatc ctcgacccga cgaaagacat atccgctatt ccgatgtttg
    24421 ggcactcaaa ccaattttgc agaccgaatt caacattgaa ggttatcctt ctcccaactt
    24481 tatgaaactg gttacaatgg taaaacctac acaggttaca ttggttcccg atcctcccga
    24541 agccattact tccaatgccg gatgggatac ggaaaaacat ctcgattttt tgaaagacat
    24601 tactgccgaa ttgcaggatg ccggaattcg tgtttccatt ttcgttgatg caaatattaa
    24661 aagtgttgaa tatgccgcat tggcaggtgc cgacagggta gaactttata ccgaacctta
    24721 tgcccgcgaa tacgcagaaa acagagaaaa ggcagttgct acatttattg aagcagccaa
    24781 agcagccaaa aaactgggat tgggactgaa tgccggacac gatttgaatc ttcaaaatct
    24841 gcattatttg catgcttgta ttccatggct cgatgaagtt tccattggtc atgccctgat
    24901 ttgtgatgct ttgtatttgg gacttcagga aacaataaaa cagtacaaaa atcagttgaa
    24961 atagtaaggc atgaattttg aaacaaaaac aaaaaaataa aaattgaaat aattatgatt
    25021 aatctgctta ttcttcaagc ttcagcaaat gttgccacta cagttgccga cccgctacag
    25081 tcggaaataa acctatggac aatggcgaaa tttggtggtc ccattatgat aattttggct
    25141 ttaatgctgg cttttgccgt atatttattt atagaaagac tgatcacgat caatcaagct
    25201 tccaaagaag atgctttttt tatggaaaga atcaaggact atatttatga aggaaaaatt
    25261 gaaactgcat taaaactctg cagggacaca aattctccca gtgcaagaat gattgaaaaa
    25321 ggaatcaccc gattggggca tcctttgaat gatgttctgg tagccataga aaatgttggt
    25381 aatatggaaa ttgcaaaact cgaaaaggga ttagttatta tggcttctat ttcgggtggt
    25441 gctcccatgc tgggtttttt gggcaccgtt accggaatgg tatcaacttt tttcaatctt
    25501 tctcaaaaag caggtggaaa catcttactt ggtgatcttt ccgttggtat gtatcaggcc
    25561 atggttacca ccattggcgg tttgattgtt ggaattttgg catattttgc ctataattac
    25621 cttgtatcga gagtagattc tgtagtaaac atcctggaat cgaaaactat ggaatttttg
    25681 gatttgctca atgaacctgt aaaataaata ttttaaaaca ttcgcagcat ggcattaaaa
    25741 aaaagaaata aagttgaagc aggtttcgcc atgtcctcca tgacagacat tattttcctg
    25801 ttattgcttt tttttgttat tgcatcaact ttatcgtctc ctaacgacat aaaattaaat
    25861 ttgcctgaaa gtagatctaa gtcttcaacc aagcaaataa ttgctaaagt tgctattgat
    25921 gctgaaggaa agttatcggt agccaaagga aatcaaaaac ccgttcctat tgaacctaaa
    25981 ttgttggaat cgtatctttt aaatatggcc tcacaagaca gtatttcttt tgttgcactt
    26041 tatgccgatc aaaatattgc ttacaaggaa atagtaaggg ttttggatat cgccaatcaa
    26101 catggtttta aaattgttat agccaccaaa ccgtttgaaa attgagaagg tgtgaaaaaa
    26161 tccgaattat atggtattgt tgggtcaatc atcttttgta tgatgctgct ggtgctattg
    26221 ctgatcatta aaatgcacgc ttcaacatct gttgaacctg aaggcattat ggtcagtttt
    26281 ggtgaaactt tggaaggagg cagcagggca gcttcccctt ctgctgcaga ggagtatgca
    26341 gtatcgaaaa aagcaagtga aaaaattttg actcaaaaag atccttctgt ttcgctcgac
    26401 aaaaagactg acaacagaga gcaggaaaga gaattgtccg agaaaaaaag acttgaacaa
    26461 gaggcaatat caaaagccaa tattatggaa gggatatttg ataaaaagat atcagggaat
    26521 gaaaatgtgg gcgatgagaa tttattaaaa ggaaatcctg ccggaagagg aacttcaaat
    26581 gggaacagct ggtcgcttag tggacgaaat ttggtgggaa atctgattac ccccagatat
    26641 gaacaaaatg tggaaggtga ggttactgtc aacattcggg ttgacgaaaa cggagttgtt
    26701 gagggagttt ctattggtgt tcccaccaat atttccgatg caacaatttt aaaagcagcc
    26761 atggaagcag caaaaaatac ccgtttcagt cctggaaagg ggaaagctac cggaaccatc
    26821 acctatcatt tcagattaaa ataaactacc ggaatgtggc tgttttacag aaatatgtgg
    26881 aaaaataaaa aacgacctta acataataaa aatgacaaac ttatgaaagc aatggtattt
    26941 ttagctgatg gttttgaaga aatcgaagcc atcgctccaa ttgacattat gcgtcgagca
    27001 ggaatagaag ttacaaccat ttccgtttca cgcgaaaaag aagtgagagg tgcacatggt
    27061 gttttggttg aggcagatcg tttgtttgaa gaagtcagtt tttctgatgc cgatttgctg
    27121 tttctgccgg gcggaatgcc ggggacaaaa aatttaaatg ctcatgacgg tttaaaacaa
    27181 cttttgctga aacatgccaa tgaaggcaaa aagttggcgg ctatttgcgc tgcaccttcc
    27241 atattgggcg aattgggatt gctgaaggga aaggaagccg tttgttttcc cggatttgaa
    27301 gaaacactaa agggtgctat tgtatccgat aaaaaagttg ttcagtcagg gaatattatt
    27361 accggtaaag ctgccggcgc cgccatagaa tttgccctga aactggttga aaatctgaaa
    27421 ggaaaatctg cagccgagca ggtaggtttg tctatttttg ctttttaaag aaaagctaaa
    27481 aacaaaaaag caaaagagtt attacaaaaa caaaggagga attaaaaaaa taattcctcc
    27541 tttattaaaa ctttaacaaa caaattaatt gatagcatcg cttaattctg caccgg
//
LOCUS       DAOSCH010000014        26627 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1324_length_26627_cov_5.815257, whole genome shotgun sequence.
ACCESSION   DAOSCH010000014 DAOSCH010000000
VERSION     DAOSCH010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26627
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1324_length_26627_cov_5.815257"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1443
                     /locus_tag="PLZ54_02350"
     CDS             100..1443
                     /locus_tag="PLZ54_02350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOJ65814.1"
                     /translation="MAVVVIMPKQGQSVESCIVTEIKKKKGDTVKKGDILFSYETDKA
                     SFEEESPVDGVILECFHEEGDEVPVLQNVMIIGEPGENYSDLLAQTNISESVKEEPEI
                     KTRQSVAEVESSPAQPALAEPQSKNNAPISPRAKYLAEKEAIETSALTGSGPNGRIIE
                     KDVRAALENRPKLTPLAKKLASEEGLQPTTSGSGLNGIAKSVDLSAPTNAVYGLDYED
                     RKLPNIRKLIAKSMQASLQNSAQLTHHLGADARCILQLRKKAKAAYESGQLQVNITLN
                     DFVCFAVIKALQKFPNVNSHFLGDSIRYFKKIHLGLAVDTDRGLMVPVVRNADDLSII
                     GLASQLKEVAALCRKGNVNPEILSSEAGTFTVSNLGNYGVEIFTPIINLPQSAILGVN
                     TIVPRPKDLGDGVYGFLPYIGLSLTYDHRSIDGGEATRFLKQIAVEIENLEIELF"
     gene            1457..2815
                     /gene="lpdA"
                     /locus_tag="PLZ54_02355"
     CDS             1457..2815
                     /gene="lpdA"
                     /locus_tag="PLZ54_02355"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOJ65815.1"
                     /translation="MTDLLIIGGGPAGYVAAERAGHHGLSVTLFEKKALGGVCLNEGC
                     IPTKTLLYSAKTYENALHADKYGVQTENVGYDYEKIISRKNKVVRKLVAGVKSKMAAN
                     KVTVIEGAATIVGRGQDGIEVSCNGNIYKGKNLLICTGSEATVPPIPGLKEAGDVVVT
                     NREILELKQQPKSLVVIGGGVIGMEFASFFNSLGTKVTIVEMLPEILGGLDFEISAML
                     RDIYAKKGIDFHLNAKVVQVDGHKVVFEKEGKTQTVEGEKILLSVGRRPVTQGFGLEN
                     LNVELNRGGIKTDEKMCTNVPGVFAAGDVTGFSLLAHTASREGEVVVNNLVGRQDRMR
                     YNAIPGVVYTNPEVAGVGETEESAKAKGIAYKVAKLPMSYAGRFVAENEGGNGLCKVL
                     VGEKYGEVIGVHLLGNPSSEIIYGACMAIEQEMTIKELQEVVFPHPTVSEIIKETIMS
                     EL"
     gene            2898..3326
                     /locus_tag="PLZ54_02360"
     CDS             2898..3326
                     /locus_tag="PLZ54_02360"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOJ65816.1"
                     /translation="MSSTIKTEYKYSELTSRIIGCALEVHKHLGNGFQEVIYQRALAY
                     EFELNGLQFHREFDMPVFYKEIQVGLRRVDFLVENVISVEIKAIIKLEDVHLAQAINY
                     LEAYNLEIGLLINFGERSLNFKRLTNKKYIPICHNNQLIK"
     gene            3345..5804
                     /locus_tag="PLZ54_02365"
     CDS             3345..5804
                     /locus_tag="PLZ54_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOJ65817.1"
                     /translation="MPKSEFIDPNQVRQPGFIEFQAIPVNQYQKTVKDERSNFTDDEF
                     KSMYHDMVLIREFETMINLIKTKGEYNGTPYNHPGPAHLSIGQESAAVGMAWTLTVED
                     FIFGSHRSHGEILAKGMSAIHKLDDEQLMQIMENFFDGTILKVVQKDFNGTTKELARR
                     FLVYGTLAEIFARETGFNKGLGGSMHAFFTPFGVYPNNAIVGGSGDIAVGAALFKKVN
                     RKPGLVVANIGDAALACGPVWEGITFSAMDQFKQLWEGDMKGGLPIIFNIMNNQYGMG
                     GQTCGETMGYEIAARIGAGVNPEQMHAERVDGYNPLAVIDAYKRKRKVIEEKKGPVLL
                     DVLTYRYSGHSPSDASSYRTKEEVEAWEKHDCIVSFGKQMIEAGVATQDDLDNVWKDV
                     KALVYELFLKTIDDNISPRLKNPEVIGDMMFSNGSVDSFSDAKPEVLMPLEENSRVKK
                     IAAKERFAFDAEGKPYSKMKQFQLRDAIFEAIMDRFYKDASLIAYGEENRDWGGAFGV
                     YGGMTEALPYHRLFNAPIAEAAIVGTAVGYAMCGGRVIPELMYCDFLGRAGDEIFNQL
                     PKWQAMSGNVLKMPVVVRVSVGSKYGAQHSQDWTSLVSHIPGLKVCFPATPYDAKGLM
                     NAALQGTDPVVFFESQRIYDIGEQFHKGGVPTGYYEIPLGEPDIKKEGKDITFLTIGY
                     TLYQALEAAKELEEKYGMSVEVIDARSLVPFNYEKVIESVKKTGKIIIASDACARGSF
                     LNDMATNISQLAFDYLDAPVCVLGSRNWITPAHELETAFFPQASWFIEMIHQRIQPLK
                     GYVPMQNFTDGEMERRAKKGI"
     gene            5901..6911
                     /locus_tag="PLZ54_02370"
     CDS             5901..6911
                     /locus_tag="PLZ54_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65818.1"
                     /translation="MEQPLSEKNTKAQILEAYEKLLKKVEQKSNDNPKEVQQRKQDVQ
                     TVEKATKTTEEEIETQIAKIKSDFVSALEIIEKELADERKKLETIQNAIRIEEKRLDD
                     LYGISVNADSLSAILLAQKEQKEQFEQEMAARKEELSSQIAQTRATWEKEKAAHDEAL
                     KAEKENLAKMRRREEEEYAYNTQQKRKKEEDEYLQMKNRQEAELKERKIAFEKEVAER
                     EKQLKESENELATLRAQAESFETKLAEAVQKAQAETEKRLQSVYAYEKELREKEVEGI
                     INLKDHQIQTLQSKIKEMEQQLREASSKVDVSEKTVKDIALKAIESAGKTQFVEREKP
                     RE"
     gene            7085..8302
                     /locus_tag="PLZ54_02375"
     CDS             7085..8302
                     /locus_tag="PLZ54_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65819.1"
                     /translation="MNTELENNFFKSTSSSDSSEAVVNVNAHLHTPYSFSAFRDIDDA
                     LDRAASENVKVVGINDFYSTAGYDEWDEGCSRRRLYPLFGIEFIALDEVDQKAGLRVN
                     DPANPGRTYISGKGLRQPFFLAEPFASQLQTVREASNRQVEAMCFKLNEVLEQKKIGF
                     LIDFDQIAFELTKGLIRERHLAKALRLKVYEYCQNDASKIQSVLETIFDGKPLKSSVN
                     DWAGVENEIRSNLLKAGGSAFVPEESTAFLPVKSVCNIILAGGGIPTYPFLADDAKGN
                     YTDFENDLERVSRQLTERGFHSVEFISTRNDLHLLEKYAEYLYDQGFVVTFGSEHNTP
                     AMEPILLSARNGIPLSEKLKKINYEGACVIAAHQHLVKQGLRGFVDENGFADRSKRSE
                     FVNLGKELIDRVL"
     gene            8505..10466
                     /locus_tag="PLZ54_02380"
     CDS             8505..10466
                     /locus_tag="PLZ54_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ65820.1"
                     /translation="MKEIEQLIEISQFYGRDSRYVIAGGGNTSYKNADRIWVKASGAS
                     LATITEDGFALLDRTKLRPMSEKKYSDDVALREEEVKNDLAAATLTRDKRPSVETSMH
                     NIIEYAFVVHLHPTLVNALMCSQQAESMLKKLFGEKALFVEYTDPGYVLFKKVEEKIK
                     AFRAAYDEEPHVIWLQNHGIFVAADTIEEIKKIYSDIFSTLEKSLQKTLPSNETLASH
                     DAEKVIPAVRMMLSTNGLKTLKVRNNPIIAFFSENVENRKLIAKPFTPDEIVYCKSNY
                     LFFDGNSEETLLREAETQIPVFIEKQGYHPHVLLMKGIGLVAAGLNAKQCETILDVFE
                     DAMKVAYLAQSFGGEHPMTQNQIDFIDHWEVENYRRSVSTAKSNGRTENKTIVITGAA
                     QGFGEGIARCLVKEGANIVVADLNEETGRKTAGELNMLCGGNRVVFVKTDVTNIASLE
                     SLMYDAVCNFGAIDCYISNAGVLRAGGLDEMTPETFDFVTRINYYGYFYGVKVVSRTL
                     KLQTRFAPKNYYADIIQINSKSGLKGSKANFAYAGGKFGGIGLTQSFALELAPFRIKV
                     NAVCPGNFYEGPLWSDPDNGLFVQYLKAGKVPGAKTIEDVKTYYLAQVPMKKGCTPED
                     VTKGVLYLMEQCGETGQALPITGGQVMLA"
     gene            10861..12138
                     /locus_tag="PLZ54_02385"
     CDS             10861..12138
                     /locus_tag="PLZ54_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOJ65821.1"
                     /translation="MKTKAVRLYGKKDLRLEEFDLPKIKDDEILAKVVSDSLCMSSYK
                     AAIQGADHKRVPDNVAENPVIIGHEFAGELIEIGSKWAGQFKVGDKFSIQPALNYEGG
                     SVGILSAPGYSYPFIGGDATYVIIPNEVMEKNCLLPYHGEGFYPASLAEPLSCVIGAM
                     HANYHLKPGSYEHKMGIVDGGKMAILAGVGPMGLAAINFVLHRKDRRPSLLVVTDIDQ
                     ARLDRAASLYTVEFAASHGIDLRYINTSVMQNPIEELKTLTNGNGYNDVFVFAPVKSV
                     VELADAILGFDGCLNFFAGPSDPKFSAMLNFYNVHYAYTHVAGTSGGNNEDMVEALDI
                     MNKGLDPAGLVTHIGGLNAVSEATLHLPEIPGGKKLIYTHITMPLTSISDFEELGKTS
                     EFFRELSSICARHKGLWNVEAEAFLLANANKSN"
     gene            12161..13459
                     /locus_tag="PLZ54_02390"
     CDS             12161..13459
                     /locus_tag="PLZ54_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOJ65822.1"
                     /translation="MKQLDVRLMHPVEQINVIIKRIYKSGMTTTSGGNISIRDQNGDI
                     WITPSGVDKGSLTFNDIMCVKKDGTVIGPHKPSSEFPFHKAIYETRPELTAIIHAHPP
                     ALVAFSIAHKVPDTKIVPQAHNICGEIGFAPYGTPGSDDLGKKIANEFRNTQYKAVIM
                     ENHGVVLGGMDLMDAYQRFETLEFCCRTIVNASRLGEVNYLSDEQVARYVNHLPSNIP
                     YFMNVDYPSDERALRSEMVKIIRRACDQGLMISTYGTVSVRWRDDDFLITPKNVARWD
                     IVPEDIVQIKKGMAEVGKTPSRSVALHQRIYQLNPQINSIICTQPVNLMAHAITGTKF
                     DVRTIPESWIFLQDVPSIPFGLIYNDIDSVAEMFNHNKVILVENDSVFVTGDKLLNTF
                     DYLEVAEFSANSLVMASAIGHLCPISDQEIDDLRVAFNVQ"
     gene            13491..14225
                     /locus_tag="PLZ54_02395"
     CDS             13491..14225
                     /locus_tag="PLZ54_02395"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HOJ65823.1"
                     /translation="MQVVFSPYKIPEFNLAAEEFLFSEKTDEYLLLYVNKPCVILGSN
                     QSVTNEVNEDFCHLNGISIFRRLSGGGAVYHDEGNLNFCFVFNKKKGYSPLSSDFLQP
                     VISVLNSMEIPVEVGKRKDLWLPDGNKVSGTASHVSKCRELHHGTLLYDVDMEKLQKS
                     LIPTSATNKKAIRSVPSPVENIRSFLERSTVRTQDVNDFFITFTKLMLAYFQIRELSL
                     LLNEDIKKIYRLTISKYGRKEWNYRI"
     gene            14622..16649
                     /locus_tag="PLZ54_02400"
     CDS             14622..16649
                     /locus_tag="PLZ54_02400"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOJ65824.1"
                     /translation="MTYESEKITRKKRIDKQLKASGWLIIPYSEGMDLTILTNHAIEE
                     LQTNSGPADYALVVNGKLLGVVEAKKLEVGAANVLEQAKRYSKGANKTVGEWNEYRVP
                     FLYSSNGELIYYLDARDSKNLSRQIYSFHTPEAMETLFNSNKETALQWLKEKPINTPG
                     LRPYQKEAIQAFENNLEERKRLMLLAMATGTGKTFTTVNLVYRMLASGYAKRILFLVD
                     RKSLAAQAVTAFASFDTPRNIKFKDEYELYSQRFKKEDFEGEAYDPTVLPNSYLTNPD
                     GTKTFLYICTIQRMAINLYGKAGAFGENEENEDDAEKLSIPSHAFDLIIADECHRGYT
                     SKETNVWRNVLNHFDAVKVGLTATPASHTVAYFNKPIFNYSLQQAIEEGYLVDYDAVA
                     INSEVLMNGAFLKEGEQVGMIDTETGREQIDQLEDEREFTSTEIEEKITAPDTNQKIV
                     EELKKYTDEWEKRTGRFPKTLIFASNDIPMISHADRLVSICKSVFNRGDDFVAKITGS
                     PTVDRPLKKIKEFRNRPNPKIVVTVDMLSTGVDIPSIEFIVFLRPVKSRILWEQMLGR
                     GTRKCDEIGKTHFVIFDCFNGTLIKYFKDASYNFKIDPPGTETITIKELIEKIYNNED
                     RKANAKRLAKRLHRIEKDMSGNARDKFATYIPNGDMGKFAELLAKFFEKYK"
     gene            16649..17335
                     /locus_tag="PLZ54_02405"
     CDS             16649..17335
                     /locus_tag="PLZ54_02405"
                     /inference="COORDINATES: protein motif:HMM:NF020052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification enzyme R subunit
                     C-terminal domain-containing protein"
                     /protein_id="HOJ65825.1"
                     /translation="MAKSFNEIFESFTSVMELLRNEQFQKLLVDYERAKKVFLVGYEV
                     KDDVSSMVLFEAEGRYGLKPTDYLLAFSEFVKRKENEIAAIGILLNKPNDWNTKALNE
                     LKQKLKENNYDEANLQKAHKFVYHKETVDIISMIKHAAKDTEPLLSPDERVNQAIQKV
                     TAGKNLNEEQQKWMEYIKEHLKQNLTLDEDDFKELPVFTDRGGLNKFKKVFADEYQKI
                     IKEINNAIAA"
     gene            17346..18109
                     /locus_tag="PLZ54_02410"
                     /pseudo
     CDS             17346..18109
                     /locus_tag="PLZ54_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619513.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
     gene            18102..19502
                     /locus_tag="PLZ54_02415"
     CDS             18102..19502
                     /locus_tag="PLZ54_02415"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOJ65826.1"
                     /translation="MSDVVNKLWGFCHTLRHEGIDYNDYIEQLSFLLFLKMADEKEVH
                     LNDKYNWKALKELSGGDLMDHYTDSLKELAKEPGLLGEIFTQAQNRFNNPVSLKKLLN
                     LIDEDEWTSMDIDVKGAAFEGLLEKAASEGKKGAGQYFTPRPLIQSMVRLMKPDPRGK
                     NTFTIYDPACGTGGFLVCAYEWLLQECKGAFPREDVKRIRENTYYGQELVARPRRLAL
                     MNMYLHGVTPKIKLGDTIYNNPSSDRFDVILANPPFGTKGANQIPERDDFTVETSNKQ
                     LNFVQHIMSTLKIGGRAAIVLPDNVLFEDKAGEVFEILMQECNLHTILRLPRGTFTPY
                     AQGVKANVIFFQKGLPTEKVWIYDNRSNIESITKKDRPLTAKHFEDFEKCYGSDPNGN
                     SPRTDQGETGRFRCFTIDEIKKRNYKLDITWLKDESIEDADSLPEPNDLATEAIAELE
                     AVVDDLKEIISLLEKE"
     gene            19514..19999
                     /locus_tag="PLZ54_02420"
     CDS             19514..19999
                     /locus_tag="PLZ54_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOJ65827.1"
                     /translation="MYILECSNGMYYTGSTNNLELRLQQHQNGEGANFTKKHLPVKLV
                     YFEEFARVDEAFYREKQIQGWSRKKKEALINNQFEKLHDFAECKNETHFLNVPFDSAQ
                     GTGNDSAQGTGNDSAQGTENNSAQGTENNSAQGTAPTRLLSGAETTDSLTLLKSNGNG
                     E"
     gene            19989..21233
                     /locus_tag="PLZ54_02425"
                     /pseudo
     CDS             19989..21233
                     /locus_tag="PLZ54_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            21270..21416
                     /locus_tag="PLZ54_02430"
                     /pseudo
     CDS             21270..21416
                     /locus_tag="PLZ54_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
     gene            complement(22029..22973)
                     /gene="trxB"
                     /locus_tag="PLZ54_02435"
     CDS             complement(22029..22973)
                     /gene="trxB"
                     /locus_tag="PLZ54_02435"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOJ65828.1"
                     /translation="MTTNEKIRCLIIGSGPAGYTAAIYASRANLSPVLYQGMEPGGQL
                     TTTNEVENFPGFPEGITGPELMDKMQKQAERFGADIRFGMVTSTDLSASPYRVTIDNE
                     KIIETQSIIIATGATAKYLGLPDEQKYAGSGVSACATCDGFFYRNRPVAVVGGGDTAC
                     EEALYLANIASKVYMIVRKPFLRASKIMQERVLQHPKIEVLFEHETLGLTGEKVVEGA
                     DLVKRRGQPDEEKVHIAIDGFFLAIGHKPNSEVFKPWIETDENGYIKTIPGTPRTNVE
                     GVFAAGDVADPHYRQAVSAAGSGCQAAIEAERYLVDKK"
     gene            complement(23000..23614)
                     /locus_tag="PLZ54_02440"
     CDS             complement(23000..23614)
                     /locus_tag="PLZ54_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOJ65829.1"
                     /translation="MLLSIISIQPALSQNDPEAQRIIDSFLNTARTGAITTRFTVSVI
                     EKNGVNSYTNSGTFTLKGNRFVLEMQEMKVWFDGKTQWAYQKSGNEVTITEPSVQELA
                     QTNPMAILGNYRNKCNIRFCKTSSEYWCIQLTPKSIKNEDIAEIEVQFLKTDGIPQSI
                     TVSQKNGTRTVLMLSQFQKNAKVSEQIFTFDAKKYKDAYINDLR"
     gene            complement(23789..26233)
                     /locus_tag="PLZ54_02445"
     CDS             complement(23789..26233)
                     /locus_tag="PLZ54_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOJ65830.1"
                     /translation="MAPKKPVKNGGRTTSGKVQKKSEKKSSGSGFGKKIASFFTDERT
                     HFIVGFMLLLVMLYLMLAFVSYFFTGAADQSKIDLSWSELHDKRTLIQNWTSVTGAIA
                     AEHLINRCFGVSAFFIPIFGIWVALRLMKAHEKSVWKAFFHCAFWLVWLSVTLGLTLS
                     PYFSDVLFFSLAGGHGDYAAQWLVSYIGASGSFLLMGGSFIVYLVIASASSLPFFRKL
                     FGKKNVSHTSFEETLVEPAVAVGTEIVPEEWISSGIETEVYEEFSKTQEESQTEKVAF
                     EVETPENNEESKPVDLDGNGENESLQEDPYYNLAKLGKYDPTLDLSHYHFPTLDLLKV
                     YGTDNETPIDMAEQIANKNRIINTLLNFDIEIETIKATVGPTITLYEIVPKAGVRISK
                     IRNLESDIMLSLAATGIRIIAPIPGKGTIGIEVPNNDPQIVSMHSIIASKKFQESKFE
                     LPIAFGRTITNEIFMVDLVKMPHLLVAGATGQGKSVGLNAIITSLLYKKHPSELKLVL
                     VDPKKVEFNIYAGIEKHFLAKLPDGSEAIITDTSKVVETLNSLCGEMDMRYDLLKKAH
                     ARNIKEYNEKFILRHLNPEKGHRFLPYIVVIIDEFGDLIMTAGKEVEMPIARIAQLAR
                     AVGIHMIIATQRPSVNIITGIIKANFPARVAFRVSSMMDSRTILDAPGANQLVGRGDM
                     LFSQGNDLIRVQCAFVDTPEVENIVHFISEQQSYPTAFYLPEYVKPEGEMGVASADLS
                     KRDPLFEEAARLIVANQQGSTSLIQRKFSIGYNRAGRIVDQLEIAGIIGPNEGSKARQ
                     VLIPDEYHLEQILKQL"
BASE COUNT         8586 a         4858 c         5812 g         7371 t
ORIGIN      
        1 tttttctttt atctaatttt actttactta ttaagtcaaa ttctttcaaa aggaagaata
       61 tcccatttga aagaggcatt ttatttaaat tatttatcta tggcagttgt ggtaattatg
      121 cccaaacaag ggcaatcggt ggaaagttgt attgttactg aaataaaaaa gaaaaaagga
      181 gacaccgtaa aaaaaggaga tattttgttt tcgtatgaaa cggataaagc ctcttttgag
      241 gaggaatctc cggtagatgg cgttatattg gaatgttttc atgaggaggg agacgaagtg
      301 cccgtgctgc aaaacgtgat gattatcggt gaacccggag aaaattattc cgatttgctt
      361 gcacaaacca atatttcgga atcggtaaag gaagaacccg aaatcaaaac gcgacaatct
      421 gttgccgaag tcgaaagttc tcccgcacaa cctgctcttg cagaacctca atcaaagaat
      481 aatgctccga tttcaccccg agccaaatat cttgccgaga aagaagcaat cgaaacgtct
      541 gctcttactg gttcgggacc taatggtcgt attatcgaaa aagatgttcg ggctgcactc
      601 gaaaatcgtc ccaaattgac accgttggca aaaaaacttg cttcggaaga aggtttgcaa
      661 ccaactacat cgggaagcgg attaaatgga atagcaaaat cggtagattt atcagctccg
      721 acaaatgcag tttatggttt ggattacgaa gatcgaaaat tgcctaatat ccgcaagcta
      781 attgcaaaat cgatgcaagc ttcgcttcaa aattcggctc agttgacgca tcatctgggt
      841 gccgatgccc gatgcattct ccaacttcgc aaaaaggcaa aagctgccta cgagagcgga
      901 caattgcagg tgaatatcac gttgaacgat tttgtctgct ttgccgtgat caaggctttg
      961 caaaaatttc cgaatgtaaa ttcacatttt ctgggcgaca gcattcgata tttcaagaaa
     1021 attcatctcg gtttggcggt cgataccgat cggggattga tggtgccggt ggtgcgcaat
     1081 gccgacgact tgtccattat cggattggca agccagctta aggaagtggc agctttatgt
     1141 cgaaaaggga atgtgaatcc cgagattctt tcatcagagg cgggtacgtt taccgtatcc
     1201 aatttgggaa attatggtgt agagattttt acgccaatca tcaatttgcc tcaatcggca
     1261 attttgggag tgaatacaat tgtgcctcgc ccgaaagatc tgggagatgg agtttatgga
     1321 tttctcccat acatcggatt gagcctcacc tatgatcatc gttcgattga cgggggcgag
     1381 gctacacgct ttttaaaaca aattgcggtg gaaatagaaa atctcgaaat cgaacttttt
     1441 taaattgaaa caccttatga cagatttatt aattatcggc ggcggcccgg caggatatgt
     1501 ggcggctgaa agagcaggac atcatggatt gtctgtgacg cttttcgaaa aaaaggcctt
     1561 gggcggagtg tgcctcaatg aaggttgtat tcccacaaaa actttgctct acagtgcaaa
     1621 aacctacgaa aacgcattgc atgcagataa atatggcgta cagaccgaaa atgttggata
     1681 tgattacgaa aagatcatca gtcggaaaaa taaagtagta cgaaaattag ttgcaggcgt
     1741 caaaagtaaa atggcagcca acaaagtaac cgttattgaa ggcgctgcca caatagttgg
     1801 tcgtggacaa gacggaatcg aagtatcctg caatggcaat atatataaag gtaaaaacct
     1861 gctgatttgc accggctccg aggcaaccgt gcctcccata cccggattaa aggaagcagg
     1921 cgatgtggtt gttaccaata gggaaatact tgaactgaaa cagcagccaa aatctctcgt
     1981 tgtgatcggg ggaggcgtta tcggaatgga gtttgccagt tttttcaata gtttgggaac
     2041 gaaagttacg attgtggaaa tgttacccga aatattgggc ggactcgatt tcgagatttc
     2101 ggccatgctt cgtgatattt atgccaaaaa aggaattgat ttccacttga atgcgaaagt
     2161 ggtacaggtg gacggtcata aagtagtttt cgaaaaagag ggcaaaacac agacggtcga
     2221 aggagaaaaa atccttctta gtgtaggtcg tcgtcctgta acgcaaggat ttggattgga
     2281 aaatctgaat gtggaactaa acagaggcgg catcaagacc gacgaaaaaa tgtgcactaa
     2341 cgtacccggc gtcttcgcag ccggcgatgt tacgggtttc tctctgctgg ctcatacggc
     2401 aagtcgcgaa ggagaagtgg tggttaataa tcttgtcgga cgtcaggaca ggatgcgtta
     2461 taatgctatt cccggcgtgg tttacaccaa tcccgaagta gcaggtgtgg gcgaaacgga
     2521 agaatcggct aaagccaaag gcatagccta caaggttgcc aaacttccga tgtcttatgc
     2581 cggacgtttt gtcgccgaaa acgaaggtgg caatggcctt tgcaaagtct tggtcggaga
     2641 aaaatacggc gaagttattg gtgtgcattt gttgggaaat ccgtcgagcg aaataattta
     2701 tggtgcttgc atggctatcg aacaggaaat gactataaaa gaattgcagg aagttgtttt
     2761 tccgcaccca accgtgagcg agattatcaa ggaaaccatc atgtcagaac tttgatttta
     2821 ttgatcaaat gattcttatg attaaatcgc acatcatctt tcagtatagt atagtcattt
     2881 tttaaattga ttacagcttg agttcaacaa ttaaaacaga atacaaatat tccgaactca
     2941 cctctcgaat tattggatgt gctttggaag tacacaagca tttaggcaat ggatttcaag
     3001 aggtaattta tcagcgtgca ttagcatatg aatttgaatt gaatggatta caattccatc
     3061 gagagtttga tatgcctgtt ttttacaaag aaatacaagt cggtttacga cgagtagatt
     3121 tcttggtaga aaatgtaatt tcagtcgaaa taaaagctat catcaaattg gaagatgttc
     3181 atcttgctca agcaataaat tatttggaag cgtataactt agaaattggc ttactgatta
     3241 atttcggtga gcgaagttta aattttaaaa gattgacaaa caaaaaatat ataccaattt
     3301 gtcataataa tcaattaatc aaataaataa tagttcaaac tatcatgcca aaaagcgaat
     3361 ttatcgatcc caatcaagta cgacaaccgg gatttatcga atttcaagct attcctgtca
     3421 accagtatca aaaaacggta aaggatgaac gaagcaattt tacagatgac gaatttaagt
     3481 ccatgtatca tgatatggtt ttgattcgcg agttcgaaac catgatcaat cttatcaaaa
     3541 ccaaaggtga gtataacgga acgccctata atcatcccgg gcctgcacat ctttccatcg
     3601 gtcaggaatc tgcagcagtc ggcatggctt ggacgcttac tgtcgaagat ttcatttttg
     3661 gaagtcaccg ttcgcatggt gaaattcttg caaaagggat gtcggcaatt cataaactcg
     3721 atgatgagca attgatgcaa atcatggaga atttcttcga cggaactatt ttgaaagtcg
     3781 ttcaaaaaga tttcaacgga actacaaaag aactcgctcg ccgattttta gtatatggaa
     3841 cgcttgccga aatatttgcg cgcgaaacgg gattcaataa aggtttaggt ggatcgatgc
     3901 acgcattttt cacccccttc ggcgtttatc ccaacaatgc cattgttggt ggttcgggcg
     3961 atattgccgt tggcgctgcg cttttcaaga aagttaatcg caaacccggt ttggtcgttg
     4021 ccaatatcgg cgatgccgca ttggcttgtg gccctgtgtg ggaaggaatt actttctcgg
     4081 cgatggatca attcaaacag ctttgggaag gcgatatgaa gggcggatta cccatcattt
     4141 tcaatatcat gaataatcag tatggcatgg gcggacaaac ttgtggcgaa acgatgggct
     4201 acgagattgc agcacggatt ggagcaggcg tcaatcctga gcaaatgcat gccgagcgcg
     4261 ttgacggata caatccgctt gctgtcatcg atgcttataa acgtaaacga aaagttattg
     4321 aagagaaaaa gggacctgtc ttgctcgatg tgcttactta tcgctatagc gggcattcgc
     4381 cttcagatgc ttcttcgtat cggacaaaag aagaagttga ggcatgggaa aagcatgatt
     4441 gcattgtttc gttcggtaaa cagatgattg aagccggagt ggctactcaa gacgatttgg
     4501 ataatgtatg gaaagacgta aaagcgctcg tttacgagtt gtttctgaag actattgacg
     4561 acaatatttc cccgcgttta aaaaatccgg aggttatcgg cgatatgatg ttttcgaatg
     4621 gctcggtaga ttctttctct gacgccaagc ccgaagtgtt gatgccactt gaagagaatt
     4681 cgcgtgtgaa aaagattgct gccaaagagc gttttgcatt cgatgccgaa ggcaaacctt
     4741 acagtaagat gaaacaattt cagctgcgcg acgccatttt cgaagctatc atggatcggt
     4801 tttataaaga tgcctcactt atcgcctacg gcgaagaaaa tcgagattgg ggaggggctt
     4861 ttggcgtata tggcggaatg acggaagcgt tgccttatca tcgtcttttc aatgcgccca
     4921 ttgccgaagc agcgattgtg ggaacggctg tcggatatgc catgtgcgga ggacgcgtca
     4981 ttcccgaatt gatgtattgc gattttctgg gacgtgcagg cgatgaaatt tttaatcaat
     5041 tgcccaaatg gcaggcaatg agcggtaatg tccttaaaat gcccgttgtt gttcgtgtat
     5101 ctgtcggttc taaatatggt gcacaacatt cgcaggattg gacatcgttg gtttctcata
     5161 ttccgggtct gaaagtttgt tttccggcaa ctccgtatga tgcaaaaggg ctgatgaatg
     5221 ccgctttgca gggaaccgat ccggtagtat tcttcgaaag tcagcgcatt tacgatatcg
     5281 gtgaacagtt tcacaaaggt ggagtgccga ccggatatta cgaaattcca ttgggtgaac
     5341 cagacatcaa gaaagaaggg aaggatatca cgtttcttac aattggttac accttatatc
     5401 aagcattgga ggcagcaaaa gaactggaag aaaaatacgg catgagtgtc gaagtcatag
     5461 acgcacgatc gttggttcct tttaattatg aaaaagtaat cgaatcggtg aaaaagacgg
     5521 gaaaaataat aatcgcaagt gatgcatgtg cgagaggttc gtttttaaac gatatggcga
     5581 ccaatatcag tcaacttgct ttcgattatt tggatgcccc ggtttgtgtg ctgggatcgc
     5641 gtaattggat tacaccggct catgaactcg aaacggcatt tttcccgcag gcaagttggt
     5701 tcattgagat gattcaccag cgtattcaac cgctcaaagg atatgttccg atgcaaaact
     5761 tcaccgacgg tgaaatggaa agaagggcga aaaaaggaat ttaaaaggac aatttctata
     5821 tacttgtatt acctttgtaa caattcaaaa aaattggata aaataaaagt aattgtttca
     5881 cccttaaaat ttatgtaaaa atggaacaac cgttatctga gaaaaataca aaagcacaga
     5941 ttttggaggc ttacgaaaaa ctgctgaaaa aagttgagca gaaatcgaat gacaatccaa
     6001 aagaagttca gcagcgaaaa caggacgtgc aaacggtaga aaaggcaacc aaaactaccg
     6061 aagaagagat tgaaacgcaa atcgcaaaaa ttaaatctga ctttgtctct gcactcgaaa
     6121 ttatcgaaaa ggaattagct gacgagcgga agaaactcga aactattcaa aatgctattc
     6181 gaattgagga aaaacgactc gatgatctgt atggcatttc ggtaaatgcc gattcgcttt
     6241 cggctatcct tttggcacag aaagaacaga aagagcaatt cgaacaggaa atggctgccc
     6301 gaaaggaaga attgtcttcg caaattgcgc aaacccgggc gacttgggaa aaggaaaaag
     6361 cggctcatga cgaagctttg aaggccgaga aagaaaatct tgccaaaatg cgtcgtcgcg
     6421 aagaggaaga atatgcttat aacactcaac agaagcgaaa aaaagaagaa gatgaatacc
     6481 ttcagatgaa aaatcgtcag gaggccgagt taaaggaacg gaagattgca ttcgaaaagg
     6541 aagttgccga gcgcgaaaaa cagttgaaag agagtgaaaa cgagttggca actcttagag
     6601 ctcaagctga aagttttgaa acgaagttgg ctgaagctgt tcaaaaagct caagccgaaa
     6661 cggagaaaag attacagtct gtttatgcct acgaaaaaga gcttcgcgaa aaagaagtgg
     6721 aaggaattat caatctgaaa gatcatcaga ttcagacgct tcaatccaaa ataaaggaaa
     6781 tggaacagca attgcgagaa gcatcgtcca aagtggatgt ttcggaaaaa acggtgaaag
     6841 atatagctct gaaggctatc gaaagtgccg gcaaaacgca gtttgttgaa cgcgaaaagc
     6901 cgagagagta agagaaccaa gagtcaagag tcaagagcct ggagtcaaga agtgaagaaa
     6961 taagaagtga gaacgtgaag agaaaggtcg catcagcaca ttattcacat cagcaaatca
     7021 acaaatcaac aaaaaagaga aggcctcaaa ggtatatctc ggtttatgct cgacacatta
     7081 aaaaatgaat acggaattgg aaaataattt ctttaaaagt acttcctcaa gcgatagtag
     7141 tgaggccgtc gtcaatgtga atgcccatct gcatacgccc tattctttca gcgcttttcg
     7201 cgatatcgac gatgcgctcg acagagctgc atccgaaaat gtgaaagttg tcggaatcaa
     7261 cgatttttac tcgacagcag gatacgacga gtgggatgag ggctgtagca gaagaaggtt
     7321 atatccgctt ttcggaatcg aatttatcgc tttggacgag gttgatcaaa aagccggttt
     7381 gcgggtgaac gatcctgcca atccgggtcg tacttacata agtggtaagg gtttgcgaca
     7441 accttttttc ctcgcagaac cttttgcttc gcaattgcaa accgttcgtg aagcttccaa
     7501 cagacaagtg gaggcgatgt gtttcaagct caacgaagtg cttgaacaga aaaaaatcgg
     7561 attcctaatc gatttcgatc agattgcttt tgagttgaca aaaggattaa tccgcgaacg
     7621 acatttggca aaggctctca ggttgaaggt ttatgaatat tgtcaaaacg atgcaagtaa
     7681 gattcaatcg gttttggaga cgatttttga tgggaaaccg ctgaaatctt cagtgaacga
     7741 ttgggccgga gtggaaaatg aaattcgttc gaatttgctc aaggcgggtg gctcagcatt
     7801 tgttcccgaa gaatcgacgg cgtttcttcc cgttaaatcg gtttgtaata ttattctggc
     7861 aggaggagga attcctactt atccgtttct ggcagatgat gcaaaaggca actataccga
     7921 tttcgaaaac gatttggagc gcgtgtctcg tcaacttacc gaaagaggat tccattcggt
     7981 agagtttatt tcgactcgaa acgatttgca tttactcgaa aagtatgccg aatatcttta
     8041 tgatcaaggc tttgtggtta ctttcggaag tgaacacaat acgccggcaa tggagccgat
     8101 tttgctttcg gctcgaaatg gaattccgct ttctgaaaaa ttgaaaaaaa taaattacga
     8161 gggtgcatgc gtaattgccg cccatcagca tttggtaaaa caaggcttgc gaggttttgt
     8221 ggacgagaac ggttttgccg atcgttccaa acgtagcgaa tttgtgaatt tggggaagga
     8281 attaatcgac cgagttttgt gatttggatt gaagaattta cgccacaagg acgcgaaggc
     8341 gcaaagaaac aaaaagaatt tgaggaactt agtgtcttag tgccttcgtg gcaattaaaa
     8401 aatttatttg atcgctgatg agtttgtttc ccaaaaacgc gaagtcacga aaaaaagaca
     8461 aaagaattta gagaaaattg acccataggg agcaaaaaac aaaaatgaaa gagatagagc
     8521 aattgataga aatatcgcaa ttttatggcc gagacagtcg gtatgtgatt gcgggaggag
     8581 gaaatacttc gtataaaaat gcggatagaa tttgggtaaa ggccagtgga gcatcgcttg
     8641 cgaccatcac cgaagatgga tttgccctgc tcgacaggac aaaacttcgt ccgatgtccg
     8701 aaaaaaaata cagcgacgat gtcgctttgc gcgaagaaga ggttaaaaac gacctggcag
     8761 ccgccacgct taccagggat aagcgtccgt cggtggaaac ttcgatgcac aacatcatcg
     8821 aatatgcttt tgtcgtgcat ttgcatccta cgctcgtgaa tgcactgatg tgttcgcaac
     8881 aagccgaatc catgctaaaa aaacttttcg gtgaaaaagc actttttgtg gaatataccg
     8941 atccgggtta tgtgttattc aaaaaagtag aagaaaaaat aaaagcgttt cgcgctgctt
     9001 acgacgaaga gcctcatgtt atctggttgc aaaatcacgg aatatttgta gctgcagata
     9061 ccatagaaga aatcaagaaa atttattctg atattttcag cacactcgaa aaatcgttac
     9121 aaaaaacgct tccttcgaac gaaacccttg caagccacga tgcagaaaag gtgattcctg
     9181 cggtcagaat gatgctttca accaatggat tgaaaacact aaaagttcgc aataatccga
     9241 taatagcgtt tttttcggag aatgtcgaaa accgaaaact gattgcaaaa cctttcactc
     9301 ccgacgaaat tgtatattgc aaatcgaatt atctgttttt tgacggcaat tcggaagaaa
     9361 ctttgcttcg cgaagccgaa acgcaaattc ctgtttttat cgaaaaacag ggatatcatc
     9421 ctcatgtttt gctgatgaag ggaatcggac ttgttgctgc cggcctcaat gccaaacagt
     9481 gcgaaaccat tctcgacgtg tttgaagatg ccatgaaagt ggcttatctg gcgcaatcgt
     9541 ttggtggaga acatccaatg acgcaaaatc aaatcgattt catcgatcat tgggaagtcg
     9601 aaaattatcg gagaagtgtg tcaacggcaa aatcgaatgg tcgtaccgaa aacaaaacca
     9661 tcgtgattac aggagctgca caaggttttg gcgaaggtat cgcgcgctgt ctggtgaaag
     9721 aaggtgccaa tattgtggta gccgatttga atgaagagac aggtcgaaag acagccggcg
     9781 aattaaatat gctttgcggg ggcaatcggg ttgttttcgt gaagacggat gtaacgaata
     9841 ttgcaagtct cgaaagtttg atgtacgatg ctgtttgcaa tttcggtgcc atcgactgtt
     9901 acataagcaa tgctggcgtc cttagggccg gcggattgga cgaaatgaca cccgaaacgt
     9961 tcgattttgt tactcgaatc aattattacg gatattttta tggcgtaaag gtcgtgagcc
    10021 gaaccttaaa attgcaaact cggttcgccc caaaaaatta ttatgctgac attatccaga
    10081 tcaattccaa atctggattg aagggaagca aagccaattt tgcttatgcc gggggcaaat
    10141 ttggtggaat tggtttgact cagtcgtttg cattagaatt agctcctttc cgcatcaaag
    10201 tgaacgctgt ttgtcccggt aatttttatg agggaccgct ttggagtgat cccgataacg
    10261 gtttatttgt tcagtattta aaggcaggaa aagttcccgg tgcgaaaacg atagaagatg
    10321 taaaaacata ttatttggct caggtgccga tgaagaaagg ttgtacccct gaagatgtta
    10381 ccaaaggggt cctttatctg atggaacaat gtggcgaaac cggacaggca ctcccaatta
    10441 ctggtgggca ggtgatgttg gcatgatata aaaatcaaga atcaagagtc aagagaaaat
    10501 tgaaatatta ctttgcgcaa cttcgtgttt ttgtgtcttg gtggcaaaaa cagacacaaa
    10561 ggctccaaga cacaaagaaa acacgaaact aaaaacttaa gaatacgtta ttacgtaatt
    10621 aatattgctc gagtgtcttt tcaatcctat gtcaacctcg aaccttacga acaattacaa
    10681 caaaaactct cagaattgca gcgaatgata catgacttca aaaattctct tatgtcttga
    10741 ttcttgactc taaaaaacaa aaaaatatga aacccacatg tattacatac acaaacacga
    10801 atgaatataa tattcagaca tgtggattcc atcagacctt taaaaataaa ttatattctg
    10861 atgaaaacaa aagcagttag attatacggt aaaaaagatc ttcgtctgga agaatttgat
    10921 ttgcccaaaa tcaaggatga cgaaatttta gccaaagtag tttccgattc gttgtgtatg
    10981 tcatcctaca aagcggccat tcagggagca gatcacaaac gggtgcccga taacgtggcc
    11041 gagaatccgg tgatcatcgg tcatgaattt gcaggtgagt tgattgaaat tggttcgaag
    11101 tgggcaggcc aatttaaggt cggcgataaa ttttccattc agccggcgtt gaattacgaa
    11161 ggcggttccg taggaatatt gagtgcgccg ggatacagct atccgtttat tggtggtgat
    11221 gctacgtatg tgatcattcc caatgaagta atggagaaaa attgtttgtt gccttatcat
    11281 ggggaaggat tttatccggc atcgctggca gagcctcttt cttgtgtgat tggcgccatg
    11341 catgccaatt atcatctgaa gcccggtagt tatgaacaca aaatgggaat tgtggatggc
    11401 ggaaaaatgg ctattctggc tggtgtggga ccaatgggat tggctgctat caattttgtg
    11461 ctacacagaa aagatcgtcg cccttcgttg ttggtagtta ccgatattga tcaagcacgt
    11521 cttgataggg ctgcttcgct ctataccgtt gaatttgcgg cttcacatgg tatcgatctt
    11581 cgttatatca atacttcggt tatgcaaaat ccgatagaag aattaaaaac tttaaccaac
    11641 ggtaatggct acaatgatgt atttgttttt gctccagtga aatcggttgt tgaattagca
    11701 gatgccattc ttggattcga tggatgcctc aacttttttg caggaccaag cgatcccaag
    11761 ttcagtgcca tgttgaattt ttacaacgtt cactatgcct atacgcatgt tgcgggtaca
    11821 agtgggggaa acaacgaaga tatggtagaa gccttggata taatgaataa gggtcttgat
    11881 cctgcaggtt tagtaacgca tattggaggt ttgaatgctg tatccgaagc tactcttcat
    11941 ttgcctgaaa ttccgggtgg gaaaaaatta atttatactc atattaccat gcctttgaca
    12001 tcaatctcag attttgaaga gttggggaaa accagcgaat ttttcagaga attgtcttcc
    12061 atttgtgcaa gacataaggg attatggaat gtggaagctg aagctttttt gttggcaaat
    12121 gcaaacaaat caaattaaat taaattataa actatagaat atgaaacaat tagatgttag
    12181 gctgatgcat ccggtggaac aaatcaatgt gatcatcaaa agaatttaca agagtggtat
    12241 gaccacaact tcgggtggaa atatttctat cagggatcaa aatggcgata tatggattac
    12301 tccatctgga gtagacaaag gatcgttaac ttttaacgat atcatgtgcg tgaaaaaaga
    12361 cggaacggtt atcggtccgc acaaaccttc ttctgagttt ccctttcata aagccattta
    12421 cgaaacgcgt ccggagttga cagcaattat tcacgcccat cctcctgcac ttgtagcctt
    12481 tagtatagca cataaagttc cagacacaaa aatagttcct caagcccata atatttgtgg
    12541 agaaataggt tttgctcctt atgggacacc ggggagtgat gatttgggaa agaaaattgc
    12601 taacgaattc aggaatacgc aatataaagc ggtaatcatg gagaatcatg gggttgtttt
    12661 gggagggatg gacttgatgg atgcctatca gcgttttgaa acacttgaat tttgttgccg
    12721 cacaattgtg aatgcaagta gattaggaga agtaaattat ttatcggatg aacaggtcgc
    12781 aagatacgtt aatcatcttc cttccaatat cccatatttt atgaatgtgg attatccttc
    12841 cgacgagaga gccttgcgtt cggaaatggt aaaaattatt cggagagcat gtgatcaggg
    12901 gcttatgatt tctacctacg gcacagtgtc ggtacgttgg cgagacgacg attttttgat
    12961 tacgccgaaa aatgtggcac ggtgggatat tgttcccgaa gatattgtgc agattaaaaa
    13021 aggaatggct gaagtaggga aaacgcctag ccgttcggta gccttacatc aacgaatata
    13081 tcaattgaac ccacagataa attccattat ttgcacgcaa ccggtaaatt tgatggcaca
    13141 tgccataacc ggtacaaaat ttgacgtgcg cacgattcct gaaagttgga tttttcttca
    13201 ggatgttccg tccattcctt tcggtttgat ttataatgat attgacagcg ttgctgaaat
    13261 gtttaaccac aacaaagtca ttttggtgga gaacgacagt gtatttgtta ccggagataa
    13321 attgttaaac actttcgatt atttggaagt ggccgagttt tcagccaatt cacttgtaat
    13381 ggcttcggct attgggcatc tttgtcctat tagcgaccag gaaatagatg atttgagagt
    13441 ggcatttaac gtacaataag gataggataa aaaaagtctg aattttatca atgcaggttg
    13501 ttttttctcc atataaaata ccggaattta atttggctgc cgaagaattt cttttttcgg
    13561 aaaaaacaga tgaatatctt ttgctttatg taaacaaacc atgtgtaatt ttaggctcta
    13621 atcagtccgt aaccaatgaa gttaatgagg atttttgcca cttaaacggt atctccattt
    13681 ttcgacgatt gtcgggagga ggtgccgtgt atcatgatga aggaaatttg aatttttgtt
    13741 ttgttttcaa taagaaaaaa ggatattctc cgttaagttc agattttttg cagcctgtga
    13801 tttctgttct aaattcaatg gaaattcctg ttgaggtggg taagagaaaa gacctttggc
    13861 ttccggatgg aaataaggtt tcaggaaccg cctcacatgt gtcaaagtgt agggaattgc
    13921 atcatggtac cttgctttac gatgttgata tggagaagct acaaaaatca ctgattccaa
    13981 cctcagcaac caataaaaaa gcaattcgtt ctgttccttc accggtagaa aatattcgca
    14041 gttttttaga gagatcgacc gtaaggactc aagatgtcaa cgattttttc atcactttta
    14101 caaaattaat gctcgcttat tttcagattc gagaactctc tcttttatta aatgaagata
    14161 taaagaaaat ctatagactt actatttcaa aatacggccg gaaagaatgg aattatagga
    14221 tatgaaactt ttccaaacca aaaaattaga ttgtattgtt cattaatttc atcagaatat
    14281 aatttatttt ttaaatgtct gatggaaccc acatgtctga atattgtatt cattgtggtt
    14341 gcgtatgcac aagcggtgaa aagacaacga cacagctaat tgaaaatgga atattaaact
    14401 tgaaatgaaa aaagaaactg acagacatat aacccgacac tcattgctta accaaatatg
    14461 ttttaatttt tttacccacc gcacaaaaat tttgaaatgc gaagcattca tgaataacat
    14521 tgaaaataaa aatagcgtta gtaaattata ttgccagccc acaaatggca catttttttg
    14581 ccattgcacc gagaattatt tgtaccttgc gatttagaat tatgacatac gaatcagaga
    14641 aaataacaag aaaaaaaaga atagacaagc agctaaaagc ttctgggtgg cttattattc
    14701 catactctga aggaatggac ttgactattt tgacaaatca cgctattgaa gaattacaga
    14761 ctaatagcgg acctgctgac tatgctttag tagttaatgg aaaacttctt ggagttgttg
    14821 aagcaaagaa acttgaagtt ggtgcagcga atgttttaga gcaagcaaaa cgatattcta
    14881 aaggagcaaa caagacagtt ggtgaatgga atgaataccg agtcccgttt ctatattctt
    14941 ctaatggaga attgatttat tatttggatg ccagagatag taaaaatctt agcagacaga
    15001 tttatagttt tcatactccc gaagcaatgg aaactttgtt taattcgaac aaagaaacag
    15061 ctttacagtg gctaaaagaa aaaccgataa atactcctgg actaagacct tatcaaaagg
    15121 aagcaataca agcatttgaa aataatttag aagaaagaaa acgcttaatg ttattagcaa
    15181 tggcaactgg tacaggaaaa acttttacca cagttaacct tgtttatcgg atgttggctt
    15241 cgggttatgc taaacgaatt ttatttttgg ttgaccgaaa atcattggct gctcaagccg
    15301 ttactgcttt tgcttcgttt gatacaccaa gaaatattaa attcaaagac gaatacgaat
    15361 tgtattcaca gcgatttaaa aaagaagact ttgaaggcga agcatacgac ccgactgttt
    15421 taccaaattc ttatttaact aatcctgatg gaacaaaaac atttctttat atctgcacca
    15481 ttcagagaat ggcaataaat ctttacggaa aagctggtgc ttttggtgaa aatgaagaaa
    15541 acgaagatga tgctgaaaaa ctttctattc catcacatgc ttttgattta attattgctg
    15601 acgagtgcca tcgtggttac acatcaaaag aaaccaatgt ttggagaaat gttttaaatc
    15661 attttgatgc tgttaaggtt ggtttgactg ctacgcctgc atcacatact gttgcttatt
    15721 tcaataaacc gattttcaat tattcgttac aacaagcaat tgaagaaggt tatttggtgg
    15781 actatgatgc tgttgccatc aattcggaag tgcttatgaa cggagcattt ttgaaagaag
    15841 gcgaacaagt gggaatgatt gatactgaaa cgggcaggga gcaaattgac caattggaag
    15901 atgaaaggga atttacttca accgaaatag aagaaaaaat cacggctccc gacaccaatc
    15961 agaaaatcgt tgaagaactt aaaaaatata ctgacgaatg ggaaaaaaga acaggtcgtt
    16021 tccctaaaac attgattttt gcttcaaacg atattccaat gatttcacat gccgaccgtt
    16081 tagttagtat ttgcaaatca gttttcaatc gaggtgacga ttttgttgca aaaattacag
    16141 gaagcccaac ggttgacaga cctttgaaaa aaatcaaaga gtttcgtaac cgacccaatc
    16201 cgaaaattgt tgtaacagtt gacatgctta gtacaggcgt agacattcct tcaatagaat
    16261 ttattgtttt tcttcgtcct gtaaaatctc gcattctttg ggaacagatg cttggcaggg
    16321 gaaccagaaa atgtgatgaa attgggaaaa cacattttgt aatatttgat tgttttaatg
    16381 gtactttgat aaaatatttt aaagatgcca gttataactt taaaattgac ccgcccggaa
    16441 cggaaactat tacaattaaa gaactcattg aaaaaatata caacaatgaa gacagaaagg
    16501 ctaacgctaa gcgtttagca aagcgacttc ataggattga aaaagatatg agcgggaatg
    16561 cccgtgataa atttgcaacc tatattccca atggcgacat ggggaagttt gccgaactat
    16621 tagccaaatt ttttgaaaag tataaataat ggcaaagtcg tttaatgaaa tatttgaaag
    16681 cttcacatca gtgatggaac tactcagaaa cgagcagttt caaaaactat tggtggacta
    16741 cgaaagagct aaaaaggttt ttcttgttgg atacgaagta aaagacgatg ttagttcaat
    16801 ggtcttgttt gaggcagaag gcagatatgg tttaaaacca acagattatt tacttgcttt
    16861 ttcagaattt gtaaaacgta aagaaaatga aattgcagca atcggtattt tattgaataa
    16921 gccgaatgat tggaatacta aagcattgaa cgagctgaaa caaaaactga aagaaaacaa
    16981 ttatgatgaa gccaatttgc aaaaggcgca taaatttgtt taccacaaag aaactgttga
    17041 tattattagc atgattaaac atgcagcaaa agatactgaa cctttgttaa gtcctgatga
    17101 aagggtgaat caagcaatac agaaagttac agcaggtaaa aatttaaatg aagaacaaca
    17161 aaaatggatg gaatacatca aagaacattt gaaacaaaac ctgactttag atgaagatga
    17221 ttttaaggaa ttgccagtgt tcactgacag aggcggttta aacaaattta aaaaggtttt
    17281 tgcagatgaa tatcaaaaaa ttattaaaga aataaacaat gcaattgcag cataatgtgt
    17341 aagttatgaa gaaggaactc attttagaat tgtttcagaa gtttgaaaac atttgttatt
    17401 tgtacaatga cgtggaatgt tggagcgcaa gagaactaca agctctgctg ggttatagca
    17461 aatgggaaaa ttttgagaaa gtaattgtta aagcaaaaga atcctgttct aatgcgggtg
    17521 aaaatataga ggatcatttt cctgacgtca ggaaaacgat acctatgcct aaaggagctg
    17581 aaaaacagat agatgatatt ctgcttaccc gatatgcctg ttatttaatt gcacaaaatg
    17641 gcgatgagag aaaggaagaa atttcattta cacagaacta ttttgctgtt caaacccgca
    17701 gggcagaatt gattgaaaac cgattactgg aatttgagcg tgtaaaagcc agagaaaaat
    17761 tatcacaaac cgaaaaacaa ctttcgggat gggaacaata caactgaaaa agaaattagg
    17821 cgtacctgat agtcgtcccg ttgcagattt tttaccgacc ataagcataa aggcaaaaga
    17881 tttggccgct gagatgacag ggttgaatgt gcaaaataaa gatttaaaag gacaaagttt
    17941 aatagaaaaa gaacatgttg acaataacct tgctgtccgt gaaatgttaa cgaaaagagg
    18001 aatatttccc gagaatcttc cacctgctga agatgttaaa aagttgcaac gtaaattgga
    18061 aggcgaagag aaaaaaatac tgacaaaaaa aagaagaaat aatgagtgat gtggtaaata
    18121 aattgtgggg cttttgccat acattaagac acgaaggaat tgattacaac gattatatcg
    18181 aacaactttc ttttcttctt ttcttaaaaa tggctgatga aaaagaagtc catctgaacg
    18241 acaaatacaa ttggaaagca ctgaaagaat taagcggtgg cgatttaatg gaccactata
    18301 ccgactcgtt aaaagaactg gctaaagaac cgggactact tggcgaaatc tttacgcagg
    18361 cacaaaaccg ttttaacaat ccggtaagtc tcaaaaaact gctcaacctg attgatgaag
    18421 atgaatggac ttcgatggac atagacgtga aaggtgctgc ctttgaaggg ctgctggaaa
    18481 aggcagccag cgaagggaaa aaaggtgctg gacaatattt tacaccgcgt cctttaatac
    18541 aaagcatggt tcgcttgatg aagcccgatc caagaggaaa gaatacattt accatttatg
    18601 accccgcttg cggtacaggt ggttttttgg tttgtgctta tgaatggctt ttacaggaat
    18661 gtaaaggtgc ttttcctcgt gaagatgtga aacgaatcag agaaaacact tattacggac
    18721 aagaattggt tgcccgtcca agacgattgg ctttgatgaa tatgtatttg catggcgtta
    18781 ctcctaaaat aaaattaggc gataccattt ataataaccc aagtagcgac cgttttgatg
    18841 tgattttggc aaatccccca tttggcacaa agggagcaaa tcaaataccg gagcgtgacg
    18901 attttacggt agaaaccagc aacaaacaac tcaattttgt gcaacacatc atgagtacgc
    18961 tcaaaatagg cggacgtgcc gccatagttt tacccgacaa tgtattgttt gaagataaag
    19021 caggggaagt ttttgaaata ctgatgcagg aatgcaactt gcacaccatt cttcgcttgc
    19081 caagaggtac atttacacct tatgcacaag gcgtaaaagc aaatgttatt ttctttcaga
    19141 aaggattgcc taccgaaaaa gtctggattt acgacaaccg cagcaatata gaaagcatta
    19201 ccaaaaaaga ccgcccgcta acagccaaac atttcgaaga ttttgaaaaa tgctatggct
    19261 ctgaccccaa tggcaacagc ccacgaactg atcaaggaga aacagggcgt ttccgttgct
    19321 ttaccattga tgaaattaaa aaacgtaatt acaaacttga tatcacatgg ctaaaagacg
    19381 aaagcattga agatgccgac agcctgcccg aacccaacga ccttgctacc gaagccattg
    19441 ccgaactgga agctgtagtg gatgacttaa aagaaataat tagtttatta gaaaaagaat
    19501 aatgaaaggg tggatgtata ttttagaatg cagcaacggc atgtattata ccggcagcac
    19561 taataattta gaattacgat tgcaacaaca tcaaaatgga gaaggtgcta attttactaa
    19621 aaagcattta cctgttaagt tagtttattt tgaagaattt gcaagagttg atgaagcttt
    19681 ttatagagag aaacaaattc aaggctggag caggaagaaa aaggaagcat tgatcaataa
    19741 tcaatttgag aaacttcatg attttgctga atgtaaaaat gagacacatt ttttgaatgt
    19801 acccttcgac tccgctcagg gtactggaaa tgactccgct cagggtacgg gaaatgactc
    19861 cgctcagggt acggaaaata actcagctca gggtacggaa aataactccg ctcagggtac
    19921 ggcacctacc cggttgttga gcggagccga aacaaccgat tcattaaccc ttttgaaaag
    19981 caatggaaat ggagaataat ttgccaaaag gttgggttga atgcagtatt ggtgaaattc
    20041 tgactgccaa aaaaggcaag aaaccggctt caacaattga cgaaccgaga gaaggttacg
    20101 ttccttacat tcttattgat gaaatggaag gaaaacaaat tagaacatat acaaatgacc
    20161 ctaaagtatc aataattgac gaaacagaag ttttattagt ttgggatggt agcattggta
    20221 aatgtggtag tgggttgaaa ggggcaatag gttctacttt agttggctta aaggctttag
    20281 gaggtattca aacaaaattt cttgaatata caatcagaca acaaaataat ttcattaaag
    20341 aaacaagcac aggaactggg ttgcaacata tcaataaaga cttttttgaa atatgcaaaa
    20401 tcccactccc ccctctcgct gagcaacatc gcattgtagc caaacttgat gctttatttg
    20461 aaaaaataga aaacaacaaa caacgattag aaaaaatacc aaaactatta aaacgcttcc
    20521 gacaatcagt tttagcggct gcggtaagtg gtaagttgac ggaggagtgg agggagaaga
    20581 ataaaaacat tgaatgtgtg gatttaattc ttgataaaat aaataaggaa aaaattaagt
    20641 atgttaaaaa gataaaagaa tatattcccg tttctgaata cgaaacaatt gaattgcctg
    20701 atacttggcg attaggaaga tgggataatt atttggattt ctccgataac ccattcagaa
    20761 gaggaccttt tggaagtgtc ttgaaaaaag aaatattcat atcaagtgga tataaaattt
    20821 atgaacagta ttgtccaata aatgatgatt gttcatttgc aagatatttt attgatgaaa
    20881 agaaatataa tgagcttaag aattttgcag ttaaagctgg tgatttttta attagctgtt
    20941 caggtgtaac tttaggtaga attactcaag ttccagataa atttgacgat ggcattatta
    21001 atcaggcaat tttaagagta aggattaatc aaaaatattt tgacagcaag ttcttccttt
    21061 tactttttag gtctccgtat tttcagcgta atatatttga aaactctacc ggctctgcta
    21121 tcccgaatgt aaaaggtgtt gaagaattga aatctatacc agctccgatt ttatctcttg
    21181 aagaacaaaa agaaatcgtc caccgcgtcg aacaactctt tgcctttgcc gataaaatag
    21241 aagcacgtta caccaaagcc aaagccatgt tggataaact accccaaagt attttagcca
    21301 aagcatttcg tggcgagctg gtggcacaag acccaaacga cgagccggca agtgtattgc
    21361 tcgaaagaat aaaagccgag aaagagaaat tggcagcaga aaagaaactc atacggcaag
    21421 cgcagggcaa gggcaagaaa acaaaggaat attcaattga ggaaaaatcg gtaaaaattg
    21481 ctgcggagaa aaagattaat tataaaaata aaaagaaata attgtggata catttcaaag
    21541 taagtattca acatggtttt tgccattgac tcataagcaa aagcaacaac atgaaaccca
    21601 catgtattgc atacacaaac acgaatgaat ataaatattt agacatgtgg gttccatcag
    21661 acctttaaaa aataaattat attctcatga aagtgcaatt tgactctaac atacaaacag
    21721 acaagaaccg ccagtgcata acacggtata taaaaaattg ggcagatagt gctaaatttg
    21781 aggtgtttaa ctcgcattta actttgcgcg gtttgacagt taattgctcc gaaatgccca
    21841 acttttcata taccgcaacc gtatgtaata catgtgggtt tcattttatt tgaaaatagt
    21901 gttaagaatt atttttcatt gacagaaaaa caacttcaca gcatataaat ttgtgcagga
    21961 aaaacaggta ttaactccct cattcaactt aatttccgac agcaaaatta aaactgcttt
    22021 actaactttt attttttatc caccaaatat ctttcagctt cgatagctgc ctgacagcct
    22081 gaaccggcag ccgacacggc ttgtcggtaa tgaggatcag caacatcacc agctgcaaaa
    22141 actccttcta cattggttcg tggtgtaccg ggaatagttt tgatatatcc gttttcgtcg
    22201 gtttcaatcc agggtttaaa tacttccgaa ttgggtttgt gcccgatagc cagaaaaaaa
    22261 ccgtcaatgg cgatgtgcac tttttcttcg tcgggttgtc cgcgacgttt caccaaatca
    22321 gccccttcca ccactttttc gcctgttaat ccgagtgttt cgtgctcaaa aaggacttcg
    22381 attttcggat gttgcaaaac gcgttcctgc attattttcg aagctctgag aaatggcttg
    22441 cgtacaatca tatagacttt ggaagcaata ttggctaaat aaagagcttc ctcacaagct
    22501 gtatcgccac ctcccactac tgctaccggc cgatttctgt aaaaaaatcc atcgcacgtg
    22561 gcacaggccg atacgcctga accggcatat ttctgctcgt ccggcaatcc aagatatttt
    22621 gccgtagcac cggttgcaat aataatgctt tgggtttcaa taattttttc gttatcgatg
    22681 gtaacgcgat agggagaagc cgacaaatcg gttgacgtaa ccattccgaa gcgaatatcc
    22741 gcaccaaaac gttcagcctg tttctgcatt ttgtccatca actcaggtcc ggtaattcct
    22801 tcgggaaaac ccggaaaatt ttccacttcg ttggttgttg tcaactgacc tcccggttcc
    22861 ataccctgat aaagtacggg cgaaagattg gcccttgaag cataaatggc agcagtgtaa
    22921 cctgccggtc ccgatcctat gatcaggcat cgtatctttt cgttagttgt catttctttt
    22981 tgtagttttt taaaaatttt tatctcaaat cgttgatata ggcgtctttg tatttttttg
    23041 catcaaacgt gaatatctgt tccgaaactt tggcattttt ctgaaactga ctaagcatca
    23101 gaactgttct tgttccgttt ttttgagata cggttatgga ttgcggtatt ccgtctgtct
    23161 tgagaaattg tacttcaatt tccgcaatat cttcattctt tatcgatttg ggtgtcagct
    23221 gaatgcacca atactccgaa gaagtcttgc agaaacggat attgcacttg ttccgataat
    23281 taccaagaat tgccatcgga ttggtttgag ccaactcctg cactgacggc tcggtaatgg
    23341 taacttcatt gcctgatttc tggtaagccc attgtgtctt tccgtcaaac cacactttca
    23401 tttcctgcat ctccaatacg aaacggttac cctttaaggt aaatgttccg gaattcgtgt
    23461 acgaatttac gccgtttttt tcgattaccg aaacggtaaa gcgcgtagta atggcaccgg
    23521 tgcgtgcggt attcaaaaaa ctgtctataa ttctttgagc ttcaggatca ttctgtgaaa
    23581 gagcgggttg tatcgaaatt atcgataaca gcatagaata aatgaatgat tttacccaac
    23641 gacttgggtt atgaaatgaa aactgcataa tcagaatttt ttagtggtga tttaagttga
    23701 ttaaatccgg aaatatcgac cggaaaaagt taaaatttta aatttgttga taattaaaaa
    23761 gattatctca agcttttttg ttgagccatc aaagttgttt caaaatctgt tccaaatgat
    23821 attcgtcggg aataagtacc tgacgagcct tgctcccttc gtttgggccg ataatgccgg
    23881 caatttcgag ctggtcgaca attcttccgg cacgattgta gccaatggaa aattttcgct
    23941 gaatcaacga agtagagccc tgctgattag caacgataag tcgggccgct tcctcgaaaa
    24001 gtggatcacg tttgctcaag tcggcagacg ctacgcccat ttctccttct ggtttcacat
    24061 attcgggcaa atagaaagca gtaggataac tctgctgttc gctgataaaa tgaacgatat
    24121 tttccacttc cggagtatcg acaaaagcac attgaacacg tatcaggtcg tttccctgcg
    24181 aaaacagcat gtcgccacgt ccaaccagct gattggcacc gggagcatcg agaatggttc
    24241 gcgaatccat catcgacgaa acgcgaaaag caacccgcgc cgggaaattg gctttgataa
    24301 ttccggtaat gatattgacc gaaggacgct gtgtggcaat aatcatatga atgccgacag
    24361 cacgagccag ctgtgcaatt cgcgcaatgg gcatttcaac ttccttccct gccgtcatga
    24421 tcagatcgcc aaattcgtca atgatcacca caatgtaggg caggaaacgg tgtcctttct
    24481 ccggattcaa atgacgcaaa ataaacttct cgttatattc ctttatgttt ctggcatgcg
    24541 cctttttcag caaatcgtaa cgcatatcca tttctccgca aagagaattc agcgtttcga
    24601 ccactttgct tgtatcggtt atgatggctt cgctgccatc gggcagtttt gccagaaaat
    24661 gtttttcgat gccggcataa atattgaatt ccactttttt gggatccacc agcaccagtt
    24721 tcagttcgga gggatgtttt ttgtaaagca acgatgtgat aatggcattc agtcctaccg
    24781 attttccctg tccggtagct ccggctacga gcaggtgtgg catttttacc aaatcgacca
    24841 tgaaaatttc gttggtaatg gtacggccga aagcaatggg aagttcaaat ttcgattcct
    24901 gaaatttttt agaagcaatg atggaatgca tcgaaacaat ttgcggatcg ttgttgggta
    24961 cttctattcc aatagtgcct ttgcccggaa tgggagcaat aatacgaata ccggtagcgg
    25021 ccaggctgag cataatgtcc gattcgaggt ttcggatttt ggaaatgcgc actcctgctt
    25081 tcggtacaat ttcgtagagt gtaatggttg gcccgaccgt tgctttgatg gtttctattt
    25141 cgatatcgaa attcagcaag gtgttgataa tgcggttttt gttggcaatc tgttccgcca
    25201 tgtcgatggg cgtttcgttg tcggtaccgt aaactttgag caaatcgagc gtagggaaat
    25261 gatagtgcga caaatcgagt gtagggtcat attttcccaa tttggcaaga ttgtaatagg
    25321 gatcttcctg caaactttca ttttctccgt tgccatccaa atccaccggc ttgctttctt
    25381 cgttgttttc gggcgtttcc acctcaaaag ccactttttc ggtttgcgat tcttcctgag
    25441 tttttgagaa ttcttcgtac acttcagtct ctatgcctga tgaaatccat tcttcgggaa
    25501 caatttccgt acccactgcc actgccggct ctacgagagt ttcttcaaaa gatgtatggg
    25561 atacattttt ctttccgaaa agttttcgaa agaaaggaag cgacgaggca gaagctatga
    25621 ccagataaac gataaaactt ccccccataa gcagaaacga acccgatgca ccaatatagg
    25681 aaaccagcca ctgtgctgca taatcgccat gtccgcccgc caacgaaaaa aacaaaacat
    25741 cggaaaaata aggcgaaagc gtcagtccga gcgttaccga aagccacaca agccaaaatg
    25801 cacagtgaaa aaatgccttc caaacagatt tttcatgcgc tttcattaag cgtaaagcca
    25861 cccaaatgcc gaaaatcgga ataaagaaag ctgatacgcc aaaacaacga ttgatgagat
    25921 gctcggcagc aatggctccg gtaacggaag tccagttctg aattaaagtt cgcttatcat
    25981 gcaactcgct ccacgacaaa tcgattttgc tttggtcggc tgcgcccgta aaaaaatagg
    26041 atacaaaagc caacatcagg tacaacatta ccagcaaaag catgaatccg actatgaaat
    26101 gtgtacgttc gtcggtaaaa aaacttgcaa ttttcttacc aaaaccgctg ccggaagatt
    26161 ttttctccga tttcttctga actttgccgg aagtcgttcg tccgccgttt ttcactggtt
    26221 tttttggtgc catattttat ggttttttgc aaacaattga taattagata ttttatttaa
    26281 cgttttttga tttgttttat tttcgacaaa aaacataaca accttattta gtttgagaaa
    26341 atttgagatt ttgtttgttg tcgaaaaaat tcaatttttc atgataatct gccttctttt
    26401 actatcaaaa aaacttgtcc attaaaaatt tattgaaaaa gattttattc atgcaaattt
    26461 acaacatttt agtggtttgc gatgttttaa aattttgcaa aaacaaattt tttctgagcg
    26521 aaataatatg agcaagatac ttttatccac ccaaactaca tgaaataaag aaattcctta
    26581 aaattgacta tcctcgccgc aagcatcgag gaattcttac gattaaa
//
LOCUS       DAOSCH010000015        26600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1326_length_26600_cov_6.755170, whole genome shotgun sequence.
ACCESSION   DAOSCH010000015 DAOSCH010000000
VERSION     DAOSCH010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 26600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26600
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1326_length_26600_cov_6.755170"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..6117
                     /locus_tag="PLZ54_02450"
     CDS             <1..6117
                     /locus_tag="PLZ54_02450"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOJ65831.1"
                     /translation="VDFTFSDENCAGKNITFTPTGLPAGADYSFLWEFGDNTTSTDSV
                     AHHIFMTAIGNGTQTFTVKLTVTDNLTACTNSKQKTITLSQHPDPKLETSAESDTLNG
                     QVTFMSCSKVATEFVFTNPNENNPYIVNYTIDWGDGSPIFSESTWSLLTHTYNLGMNT
                     LKYTVQGQNGCSVTKEYKIFVGSNPAGGILSPGNTDICVNEQLTFPIAGIQNNPPGTI
                     YMVSFNDGTPEEVFYDNTRTQITHTFTNGSCGTYSYDGRNYYPNSFMVSMTAKNPCSP
                     SGTSSAVTPIYVSIPPEANFNANNSTCVNTDFFIQNTSRRGYVASSSGCTSGDKFVWS
                     ISPTEGVTVVSGSLGNTRGLNDEQYSSWISGSDNVILRFANPGNYTIKIKIANRCGAS
                     DTTQTICVEPVVTPSFTLDKNKGCAPFTVKVNNTTDTTKLCSNPSYNWTVGYQPANCG
                     TTSSFDYVDHTSTSSKNPHITFNNPGIYTLTMNVYTNCSVQTVSQQVTVTRPPLVSID
                     DIPDFCASAEISPTATIQTCIPATDSVSYEWSFPGGTPASSTAASPGKITYSSPGNYT
                     VSLTVKNDCGSTVATKTFKINVVPQITNTELSQTICSGDSSQSVPLTSTVPGTTFSWT
                     AQASTGITGFISSGTSNTIPAQNIITSANVSGNVTYSIVPKVGTCTGDTVRYVINVYP
                     RPYFTKQPVSSILCKDGAATLLSVSFSKGEGTFSFQWYSNNVKSTSGGTPIPGAVDSV
                     YTPSTSTVGTTYYYCIIEFSSGYCREIISDIATVTVNEPPQITQQPLASQIICVGGSL
                     PQPLKVKYSGGAGPSFQWYSNNVDSNVGGTAISGANDSIFTSSSFTATGTFYFYVTIT
                     FSGNNCDPVTSETAKVEVIPDPSIAQQPLASQTICQNSIAEELSVKVTGGIGKYAYQW
                     YQNTTNSNSGGTIIPNANDSIYMPSTTNTGTLYYYCEITQPNGPGCNAVSNTSQVIVR
                     EGPAITKQPSSFVVCKNEISPKLVISWKNGVGIPSYQWFSNTLNSNTGGTLIPTATDS
                     VYMPSTAVVGTIYYYCVISFSSGGCSELVSNPAAVTVNQFPVISDINVSICSGQSFNI
                     IPENSGSNIVPTGTTYTWSMPAISPSNSITGASAQSNPQPQVSQTLTNITTSIAIVTY
                     TVKPSANGCDGAEFKISVTVYPAAHIEANVHNITCNGANDGMISLTITGGVPFATGDP
                     YQITWNGPNGFTSHNANISNLSAGDYQLQVLDAGGCPYQATYTIIEPEKIKISGITQK
                     NVSCYGNADGEISFNVSGGTGNYVYSWTKDGTTFTGTNHLTALQAGTYAVSVTDGNAC
                     APQSEEFTIIEPLPLTVSLSDKKNVLCYGDATGSIDVEVTGGTKAEITSGDYDYYYSW
                     TGPENYTSNNKNLTNLKAGDYFLTVRDASNCTVTSDAFTVTQPQELIVSATSTAITCF
                     GDDNASITLNVTGGVEPYSVQWSNYGEGMVQENLSPGTYDITVTDNNGCQKSLQVIIP
                     EPFRFSVKPVVKQISCYGANDGSIKLNFVGGMPPISFAWDDDPNAGVDRNQLKPGVYT
                     VRIHDAQPCDIIRSFTIIEPQPLTVSAQVKDATDCQNVNSGSILLAVTGGRPPYKYEW
                     SNGMTTQNILNVPPGNYSVLVTDSFGCNVTVQHSIQRQLPLYVQVNSDIEFDCLTKNI
                     IQVNQATVTGGVPPYQISWSSGTISGANNEIMTTGQNGLVFVHVTDALQCEITTSINI
                     QIPEQGISYQLLDCNQRSYQFNALIPIEKADYIYSWDFGDGGVSDIKNPQYNFSKPGN
                     YTVKLKVQNAECSLYYEKTISVEAPPVLSLDKEPIFCIGDSLLLHVSGADYYRWNDGS
                     TADNLLIVKPGTYSVEGTSKAGCSSILTFMASYFDLFHYTIQTDQEMVTDRQPTIEFW
                     SEDTSNAIYYWDFGDGYKTQGNHVSHSYIISRDGYYEVNLQVINPNGCREYATKRIWI
                     GAPTLPNTITPNGDGKNDLLLKGFHIQVFNRNGLLLFDGREGWDGTYKGKPVSEGTYF
                     YVLMYATPEGLKFASNFVTVIR"
     gene            6128..7036
                     /locus_tag="PLZ54_02455"
     CDS             6128..7036
                     /locus_tag="PLZ54_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOJ65832.1"
                     /translation="MKIRFLIVIFLFAVIVSMFGQSNIRVNNYWENTYYINPASIHDQ
                     YNGEISMAARNQWINFPGSPTTFFGHGTVFLSDFHTQIGMKAMADKIGYTNTYDLAFS
                     YAYQLTLNPTWNLSMGLTVNYQGLNYDLSQVNLTNGDDPSAYEQFKFESNFNSNLGVE
                     LNNKYWKIGLAGQNIFSLFYSFSELFNNTNFLYASYKPYTRNFFSVGGGMCLIQYGNM
                     MQAELNFNGFFKMTPENNAFQLGLFYRTWHEAGALFGIDLTNSFRLSYSYDYNAGAIR
                     RNSYGTHELILTYKIKGEPKCINCWY"
     gene            7236..7739
                     /locus_tag="PLZ54_02460"
     CDS             7236..7739
                     /locus_tag="PLZ54_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOJ65833.1"
                     /translation="MAQHQIDRLDRKILQLISQDARIPFLEVARECNVSGAAIHQRIQ
                     KLRNLGIIKGSEFVIDTYKVGFQTCAYIGIILSDIKMFKSVVEELKKIPEIVECHYAT
                     GKYSMFIKIYAKDNRHLLQIILEKLTVIPGVAHTETFQISLDEIFRRQLFVFDTENEV
                     ENLEEEI"
     gene            7879..8064
                     /locus_tag="PLZ54_02465"
     CDS             7879..8064
                     /locus_tag="PLZ54_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65834.1"
                     /translation="MKQAKWWTLQQIEENLQEGIFTENFLIEFDLLKKSGVLENNICD
                     CACKLKETIFKAPHLFR"
     gene            complement(8089..8802)
                     /locus_tag="PLZ54_02470"
     CDS             complement(8089..8802)
                     /locus_tag="PLZ54_02470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOJ65835.1"
                     /translation="MEMNQIYALSCLEGLKKLPDHSIDLIFTDPPYYQYRAQNIKGLK
                     NHKDVVTEFEFDDFKSEEEYLQFLEEVLKECYRVSKPGASGYLWCGDDFVSFLNRMVE
                     RAGFQFRKVIHWHKTNPFPAVHPRKMFANSMELIVHFSKGTPKTWNYKSTNEMHNFIE
                     APICMGKERTIHRTQKPLKVCIPYIEISSNEGDLVLDPFMGSGTTAVACKMLKRNFIG
                     FEINPLYCEIANQRLQKVE"
     gene            complement(8833..9942)
                     /gene="meaB"
                     /locus_tag="PLZ54_02475"
     CDS             complement(8833..9942)
                     /gene="meaB"
                     /locus_tag="PLZ54_02475"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOJ65836.1"
                     /translation="MHFHQKHPENDPSFKNLSVNDGVNKPPIVNPYQKVKQKRKIYSA
                     SEYVKGILSGDISLLSQAVTLVESSLTEHQNIAQEIIEKCLPYSGKSVRLGITGVPGA
                     GKSTFIEALGMYLVQKGHRLAVLAIDPSSERTKGSILGDKTRMEELSVSKNAFIRPSP
                     SAGTLGGVARKTRESIILCEAAGFDRVFVETVGVGQSETAVHSMVDFFLLLQIAGAGD
                     ELQGIKRGIMEMADGIVINKCDGNNIEKAQLAKIQHQNALQLFPPAESGWKPEVLTCS
                     AIEKTGIEDVWNMVERYVEYTKANGFFEYKRNDQAKYWMYETINEQLKAHFYQNEEIR
                     KLLPEIENKILTNELTSFVAAKKLLETYFGKINKK"
     gene            complement(10024..11100)
                     /locus_tag="PLZ54_02480"
     CDS             complement(10024..11100)
                     /locus_tag="PLZ54_02480"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOJ65837.1"
                     /translation="MKKTVSIIFMMLLFVSASNAQKAVIQFQEKIHDFGQIKEEDGKV
                     SYQFKFTNTGTGPLVISRVQASCGCTTPNWTKEPIEPGKSGSIMVTYNPLGRPGIFNK
                     TITVFSNATEEQVQLQIKGEVIPKTDENSDYPISVSGIRLKTKIVQMNNVNKGTIQSR
                     SISFKNTTSQNVRITFENLPSYVTSEVIPDITQPNEEGKITFYLNSKNCQQWGPISDD
                     FYLIVNGQKKFSEDNKLTLYANIVEDFSKLSVSERQNAPILDIPSTTLSLGTFKAGSK
                     ISGKFLIKNIGKDELKIHRIINNNKELTVSSVQTIKGGSKGELKVTVNTANLASGNYK
                     KTITLQTNDPNNSFVILVVNWTIS"
     gene            complement(11314..11853)
                     /locus_tag="PLZ54_02485"
     CDS             complement(11314..11853)
                     /locus_tag="PLZ54_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ65838.1"
                     /translation="MIDFKRMQSSSDAFFQQFYRLYSSAFPENERRSLQNLEKELNNP
                     NFHAYVLMNKNSFVGIFNYWNFEKFIYIEHFAIEEKLRSQYLGSEALKSFLEKVNLPV
                     ILEVEMPNTDAAKRRIEFYHRHEFVELQNYYLQPPYESGGFPIPMQLMSNDYEFAKNN
                     FETIKQTIYLEVYHFKPEV"
     gene            complement(11893..12711)
                     /gene="nagB"
                     /locus_tag="PLZ54_02490"
     CDS             complement(11893..12711)
                     /gene="nagB"
                     /locus_tag="PLZ54_02490"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOJ65839.1"
                     /translation="MRLIIQPDYPTLSQWAANYVAAKILHAQPSAQKPFVLGLPTGSS
                     PLGMYHYLIELNKKGIISFENVITFNMDEYVGLPREHEESYYSFMWKNFFSHIDIKPE
                     NVNILNGNAEDLIAECAAYEEKMKSVGGIELFVGGIGPDGHIAFNEPGSSLSSRTRQK
                     TLTTDTILANSRFFNNDINQVPKTALTVGVGTIMDAREVLILVNGHNKARALRHAVEE
                     PISQMWTVSALQLHPKSIIVCDYDACIELKVGTYKYFLDIEKDYLNPESFNIFP"
     gene            12898..15126
                     /locus_tag="PLZ54_02495"
     CDS             12898..15126
                     /locus_tag="PLZ54_02495"
                     /inference="COORDINATES: protein motif:HMM:NF016945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase"
                     /protein_id="HOJ65840.1"
                     /translation="MKNHLLSILLLVACHQSIYASSYQSFVKNSEALIQRVLPEKANH
                     FVIEQISSEHGKDVFEIATSKNGQVVLRGNNELSVAMAFNRYLRDVAHVSYDWFSVSP
                     LQIKGELPLPISTIRMACNAQERFFNNTCTFGYTFPFWNWERWEKFIDWLAMNGVNRP
                     LMLAGQEKVWLNVWQSFGLKKEDIQAYFSGPAHLPWHRMANLDKWGGPLPVSYIEGQY
                     RLQKKILARCRELSMKPVLPAFAGHVPEQLKQIYPEANIHQISPGWGGMDADYTTYFL
                     DPSEKLFSEIQYRFLKEQEKLYGISHRYSADPFNEIDPPSWAPEYIASVAKTIYRSMK
                     NADPEAIWYQMSWTFYYDSEHWTQERLKAMVESVPKGKLIFLDYVAEEEEFYKRTENF
                     FGAPFIWCYLGNFGGNTHLVAPMKKVSKRIVEALKVANCLGVGATLEGLNVNPEIYEM
                     VLEIPCSNQISSFNLDEWIKNYAVRRAGQEDSAVIEAWKLLKEKVLVDEAQVIGNHSV
                     VFQVLPVMDLKESSWTTNPFVPYDNPDLAKALQIMLQANEESRKNDAYCFDVVNLARQ
                     ALGNYGSCLYEKMMTAYREQNLSDFQKYSHQFISLGMEIDLLLGTRKEFLLGSWLSDA
                     RRWGKTREEKIYYERNAREIITTWHKSGGELVDYANRQWNGLMRSYYLPRWEKFIELL
                     QKSLINGTSFEQEDFSAWCIDFERKWVNGEIQENFLEKPSGNAIETAQRLFEKYSTEM
                     LK"
     gene            complement(15596..16807)
                     /locus_tag="PLZ54_02500"
     CDS             complement(15596..16807)
                     /locus_tag="PLZ54_02500"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOJ65841.1"
                     /translation="MKILVLNCGSSSVKYRLLDLENNKVLGSGGVEKIGMKGSLLKHI
                     RQDNQIIILEGEVLEHQTAIEYILGVLTSAKHGCIKSLEEIDAVGHRVVHGAEKFNSS
                     VLITDEVIKKIEECIDIAPLHNPPNLAGINAIGELLPTVPQVAVFDTAFHQTMPEYAY
                     MYAIPYSLYTKYGIRRYGFHGTSHRYVSHRACEFLNMDYVQTKIITVHLGNGASITAI
                     ENGKSVDTSMGFTPLEGLMMGTRSGDVDLGIATFLMEKEMIGPSALSTLFNKHSGVLG
                     VSGISSDMRDIETAIANGNERAKLALDMYEYRIKKYIGAYAAALNGVDLIVFTGGIGE
                     NQAKTRQNIIKGLTYLGIDVDEKANAEARGKEALISSAESRVKVAVIPTDEEFMIASD
                     TMKIINEMKRG"
     gene            complement(16820..17776)
                     /locus_tag="PLZ54_02505"
     CDS             complement(16820..17776)
                     /locus_tag="PLZ54_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOJ65842.1"
                     /translation="MSQPIIEPIEKYGLSKRSRRRTLFSKIGVVGCGNEGSAIATIAL
                     LNGIEVVFLEPTEEKIANAYSRIERKLNKRIQNWGLTENEKKIALSRVQGTTKYKDFT
                     GCDFVIEAVRYDDQTGERHVEERKEVFLQLEKVLSPQAIIASNISTVVITELASELQY
                     KERCIGLHFLSNVPGSNIIEIVRGLYTSDETYEKICQFARLINHQYVAVAESAGLVSI
                     RLFLIELNEACAMLMEGISNVEDIDKVLTVGFGHRLGVFRTADQMGIEKIVKLMENLY
                     QEYGNSKYKPSPVLLRLSRAKHFGIKKGKGFYEYDEFGNVIK"
     gene            complement(17803..18801)
                     /gene="pta"
                     /locus_tag="PLZ54_02510"
     CDS             complement(17803..18801)
                     /gene="pta"
                     /locus_tag="PLZ54_02510"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOJ65843.1"
                     /translation="MDLLNKILERAKANIKRIVLPEGDETRTLQAAEIILKEKAAHLI
                     LLGKEDVILKTASNLHLNHIAEAEIIDPETSPRKAEYIDLLYNIRKNKGITYQEAEEL
                     TKNPLYFACLMIKNGDADGELAGARNFTGDVLRPAFQIIKTLPGIQVVSGAILMFTKT
                     PQYGENGLLVFADCAVNPNPTAEELAQIAVCTAKTTRQIGEIEPRIAMLSFSTKGSAS
                     HELVDKVVKATRLAREMEPTLLIDGELQADAALVPSVAHRKAPESAVAGKANVLIFPD
                     LQSGNIGYKLVERFGGAEAVGPILQGMAAPVNDLSRGCSVEDIFHMITITSNLAIK"
     gene            complement(18992..21043)
                     /locus_tag="PLZ54_02515"
     CDS             complement(18992..21043)
                     /locus_tag="PLZ54_02515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOJ65844.1"
                     /translation="MAIAVLGAGCTEKENHSVIGKQIPNIENGRMTPEVLWSFGRVGG
                     VQISPDRTKILYSVTYYSIPENKGNTELFIMNVDGTNKHQITHTATKEAEARWMKDGE
                     HIAFLSSESGSNQLWTMKIDGSERKQISNRDGGINGFLFSPDESKVLFIADVKYGKTA
                     ADKYPDLPKATGRIVTDLMYKHWDEWVESIPHPFIADFKDNTLKNEQDIMKGEPYECP
                     MKPFGGIEQLDWSPDGKIIAYTCRKKTGKEYALSTNSDIYFYYLENGKTENKTEGMQG
                     YDINPKFSPDGKWMAWESMERDGYESDKNRLFVMNVQSGEKLDLSEDFDQNCNAISWS
                     ADSRSIYFVSCWHAVTQIYKTDLRTKSIAPITHGVHDYEFVAEARNGFIGVKHSMSKP
                     DEIYFIDPVTGKEAELSFENKELLSKLKMGKVEERWITTTDKKQMQTWVIYPPDFDPA
                     KKYPALLFCGGGPQSTVSQFWSYRWNFQLMAANGYIIVAPNRRGVPGFGKAWLEQISG
                     DYGGQNMKDYLSAIDALAKEPFVNKNKLGCVGASYGGFSVYWLAGHHNKRFKAFIAHD
                     GMFNLEQQYLETDELWFVNWDLGGPYWDRKNPIVRRTYANSPHLFVDKWDTPILIIHG
                     EKDYRILASQAMAAFNAAVLQGVPAEMLIFPDENHWVLQPQNGILWQRTFFEWLDKWL
                     K"
     gene            21327..21677
                     /gene="rplS"
                     /locus_tag="PLZ54_02520"
     CDS             21327..21677
                     /gene="rplS"
                     /locus_tag="PLZ54_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOJ65845.1"
                     /translation="MDLLKIAEQSFEQEVKTYPEFRSGDTITVAYRIKEGNKERIQEF
                     KGDVIKISGHGGKKHFTVRKMSGNVGVERIFPMDSPFIESITVNKHGKVRRAKLYYLR
                     NLTGKKAKIKERRV"
     gene            complement(21763..22089)
                     /locus_tag="PLZ54_02525"
     CDS             complement(21763..22089)
                     /locus_tag="PLZ54_02525"
                     /inference="COORDINATES: protein motif:HMM:NF027794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HOJ65846.1"
                     /translation="MTKNEQTEEKLKEFFKTHKTEIPDNGFSLKVMRRLPTEKDHSWI
                     VWLIGATGISFLLYHYIHTGILLNFIENLLVMHFDMLLISIAVFPIVCGIVMLLWGKK
                     EKTFFG"
     gene            complement(22076..22621)
                     /locus_tag="PLZ54_02530"
                     /pseudo
     CDS             complement(22076..22621)
                     /locus_tag="PLZ54_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668052.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(22605..23231)
                     /locus_tag="PLZ54_02535"
     CDS             complement(22605..23231)
                     /locus_tag="PLZ54_02535"
                     /inference="COORDINATES: protein motif:HMM:NF039701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HOJ65847.1"
                     /translation="MKKLVLLVFSLILFSGIGFAQNKSDESSHPSLPAALENLTQREK
                     DSILLNKLSPEQLLELKKAELENERKKIEGNAREDMPLTGFQIFMIVVAPFLFVIILI
                     WTIYAAKNKESKRKFELYMKALEMGQPIPEDFFQKDKKDKPNSNLKKGVLWAAVGLAL
                     LIYFIINHNNDGLIVGIVPTFVGIGYLVVHFYDRSKEKQPIEQNEQQG"
     gene            23452..25737
                     /locus_tag="PLZ54_02540"
     CDS             23452..25737
                     /locus_tag="PLZ54_02540"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOJ65848.1"
                     /translation="MKTEKFDVVGMSCAACAAHIEKEVGKLEGVKKVNVNLLTNSMVV
                     DFEESNVDTDKIEEAVVEAGYEAHVKSSAEQAASRETKKIDFVQKEQQEMQKRWWITF
                     GFWLPLLYLSMGSMLGWYIPAFFRGEENAMVFAFTQFLLTLPIAMSNEKYFRNGFKML
                     FKAAPNMDSLIAIGSSAAILYGIYGLYKIAYGLGHAQPELVHQFAHDMYFESGATILT
                     LITLGKYLESRSKSQTSRAITQLMDLSPKTATVIRDGTEVEIPAEEVRVGDIIVVRPG
                     QRIPVDGVVESGSSTVDESALTGESIPVLKQSGDRVLSASINKSGYITLKATQVGNDT
                     TLSHIISLVEEASSSKAPISKLADRISRIFVPVVIFIALTAGIVWFSLGYSFDFSLSV
                     AISVLVISCPCALGLATPVAIMVGTGKGAEHGILIKSAASLEMAHKIDTVVLDKTGTL
                     TMGKPQLTDIIPSPNITENELLQIAASLEKLSEHPLAEAVLEETDKRKLETFSVKNFT
                     SLPGKGIVAEAGENYFLIGNRQLMEEQKIDVQNFDKIATQLAEEGKTPLFVASREKVI
                     GVIAAADPLKPTSKEAVNQFRSLGLEVVMLTGDHKKTAAAVQRKLGISTVFSEVLPQD
                     KDKVIVNLQKGGKKVAMVGDGINDAPALMRADLGIAIGAGTDIAIESADVVLMRNDLM
                     DAVSAIRLSNAVIRNIKENLFWAFFYNIIGIPLAAGIFYPLLGWKLSPIFAAAAMSFS
                     SVTVVLNALRLRFFKPSEVKS"
     gene            26017..26475
                     /locus_tag="PLZ54_02545"
     CDS             26017..26475
                     /locus_tag="PLZ54_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65849.1"
                     /translation="MENQINGPKKQYFEPMHTCEHILNQTMIRMFGCGRSQNAHIERK
                     KSKCDYLLPQAPTEEQIRELSDRVNAVIDSHLPVTIEFLKREEASGVVDLSKLPDDAS
                     DVLRIVRVGDYDACACIGQHVNNTSEIGHFEIISHDYEDGRWRVRFRLID"
BASE COUNT         7849 a         5125 c         5248 g         8378 t
ORIGIN      
        1 gtcgatttta ctttttccga cgaaaattgt gcaggaaaaa acatcacatt tacacctacc
       61 ggcttacctg ccggcgctga ttattctttt ttgtgggaat ttggagacaa taccacttct
      121 accgatagtg tagcacatca tatatttatg acggcaatag gaaacggcac tcaaactttt
      181 acggtaaaac tgaccgtcac tgataatttg actgcctgta ccaattcaaa acaaaaaacc
      241 atcaccttat ctcaacatcc cgatccaaaa ttggaaacat ccgcagaaag tgatacgttg
      301 aatggtcaag ttactttcat gtcttgtagc aaggtcgcca ccgaatttgt atttaccaat
      361 cccaatgaaa ataatcccta tatagtaaat tatactattg attggggtga tggttctcca
      421 attttttcgg aatccacatg gtcattactg actcatactt ataatttggg aatgaataca
      481 ttaaaatata cagttcaagg gcaaaatggt tgctccgtaa ccaaagaata caaaatattt
      541 gtaggctcca atcctgcagg tggcattctt agtccgggta atacggatat ttgtgtaaat
      601 gaacaattga cttttccaat tgccggtatt caaaataatc ctcctggtac catttatatg
      661 gtttcattta acgatggcac acctgaagag gttttttatg ataatacccg aacccaaata
      721 acccatactt ttactaatgg ttcatgtggt acttacagtt atgatgggag aaattattat
      781 cccaattcgt ttatggtgag tatgactgcc aaaaatcctt gcagcccttc aggaacatca
      841 tctgctgtta ctccaatcta cgtttctatt cctccggaag caaatttcaa tgccaacaat
      901 tctacttgtg taaataccga ttttttcata caaaacacct caagaagagg atatgtggca
      961 tcaagttcag gatgtacttc aggagataaa tttgtatggt ctatatctcc aaccgaaggt
     1021 gttactgtgg taagtggctc attaggaaat acacgagggt tgaacgatga acaatacagc
     1081 tcctggattt ctgggagtga taatgtcatt ctaagatttg caaatccggg aaactatacc
     1141 atcaaaatta agattgccaa tcgttgcgga gcaagcgata ctacgcaaac catttgtgta
     1201 gaaccggtag ttactccatc ttttacattg gataaaaata agggttgtgc tccatttacc
     1261 gtaaaagtta acaacacaac cgatacgaca aaattatgca gtaacccttc atacaattgg
     1321 actgtcggtt accagcctgc caattgcggc actacatcct catttgacta tgttgatcac
     1381 acttctacct catcaaaaaa tccacatatt acttttaaca atcccggaat atatacatta
     1441 acaatgaacg tttacacaaa ctgttctgtt caaactgtca gccagcaagt tacggtaaca
     1501 cgtccacctc ttgtttcgat cgacgatatt cccgattttt gcgctagcgc cgaaataagc
     1561 cctactgcaa ctattcaaac ctgtattccc gctaccgatt cagtcagtta tgaatggagt
     1621 ttcccgggag gaacacctgc aagttccaca gccgcaagtc cgggaaaaat tacctattca
     1681 tctccgggaa actacaccgt ttcattgaca gtaaaaaatg attgtggaag tacagttgca
     1741 acaaagacat tcaaaataaa tgttgttcct caaattacaa ataccgaatt aagccaaacc
     1801 atttgttcag gcgacagttc tcaatccgtt ccattgactt ccactgttcc gggaacaaca
     1861 ttttcgtgga cagcacaagc ctcaaccggt attactggct ttatttcttc cggaacaagc
     1921 aatacaattc cggctcaaaa tataataacc agtgcaaatg tttccggcaa tgtaacttat
     1981 tccattgtac caaaagtagg tacttgcaca ggagatactg tcagatatgt catcaacgtt
     2041 tatccacgtc cctattttac gaaacaaccc gtttccagta ttctttgtaa agatggagca
     2101 gcaacacttt tatcggtttc tttttcaaaa ggagaaggaa ctttttcttt ccagtggtat
     2161 tccaataatg taaaatccac ttcaggtggg acgcctattc caggagctgt ggactctgtt
     2221 tatacaccat caacttctac ggttgggaca acttattatt attgtatcat agaattttcg
     2281 tcaggttatt gcagagaaat aatttccgat attgctacag taactgtgaa tgaacctccc
     2341 caaattactc aacaacccct tgcatcacaa ataatttgtg tcggaggtag tttgcctcag
     2401 ccactcaagg taaaatacag tggtggtgct ggtccatctt ttcagtggta tagtaataat
     2461 gtagattcca atgttggagg aacagctatc tcgggcgcaa acgattctat atttacatca
     2521 tcttcattta ctgccaccgg cactttctat ttttatgtaa cgattacttt ttcgggcaac
     2581 aattgcgacc ccgttacaag tgaaacggca aaggttgaag ttatacccga tccgtccatt
     2641 gcgcaacaac ctttagcatc gcaaactatt tgtcaaaatt caatagcaga agaactaagc
     2701 gtaaaagtta ccggaggaat tgggaaatat gcctatcaat ggtaccaaaa caccacaaat
     2761 agtaacagtg gaggaacaat aattcctaat gcaaatgaca gtatctatat gccatccaca
     2821 acaaataccg gaactttata ttactattgt gaaattacgc agcccaatgg accaggatgc
     2881 aatgcagtca gcaatacttc gcaagtaatt gtaagagaag gtcccgctat tactaaacaa
     2941 ccttcatcgt ttgtggtttg taaaaatgaa atttcaccca agttggtgat ttcatggaaa
     3001 aatggagtag gaattccttc ctatcaatgg ttttcgaata cgttgaactc gaataccggt
     3061 ggaaccttaa ttccaacagc aactgattca gtttatatgc cttctactgc tgttgtcgga
     3121 acaatttatt attattgtgt tatttctttt tcatcgggag gatgttccga attagtttcc
     3181 aatccggctg cagtaacggt taatcaattc cctgtcattt cagatattaa cgtttcaatt
     3241 tgcagtggac aaagtttcaa tattattcct gaaaattccg gaagtaacat cgttccgaca
     3301 ggtacaactt atacatggtc aatgccggct atttctccat cgaattcaat tactggtgca
     3361 agtgcccaat caaatcctca acctcaagta agtcaaactt tgaccaatat aaccacttct
     3421 atcgctattg taacctatac ggtcaaacct tcagccaacg gttgtgatgg tgcagaattc
     3481 aaaatttcgg ttaccgtgta tccggcagct catattgaag caaatgttca caacattacg
     3541 tgtaatggag caaatgatgg aatgataagt ctgacaataa caggtggtgt accttttgct
     3601 acaggcgatc cgtatcaaat aacatggaac ggacccaacg gattcacttc acataatgca
     3661 aatatttcaa atttatcggc aggtgattat caacttcaag tgttggatgc cggaggttgt
     3721 ccctatcaag ctacgtatac aattatagaa cctgaaaaaa tcaagatttc cggaataact
     3781 caaaaaaatg tgagttgcta tggcaatgcc gacggtgaaa tcagcttcaa cgtaagtgga
     3841 ggaacgggaa attacgttta ctcatggaca aaagatggaa caacgttcac gggcaccaat
     3901 catctgactg cacttcaagc aggaacttat gccgtatcgg ttactgacgg caatgcgtgt
     3961 gctccgcaat cggaagaatt tacaattatt gaaccgctac ccttaacagt ttctctttcc
     4021 gacaaaaaga atgttctttg ctacggagat gcaaccggaa gtattgacgt ggaagtaaca
     4081 ggaggaacca aagccgaaat aacttcaggt gattatgatt attactattc gtggacagga
     4141 cctgaaaatt ataccagcaa caataaaaat ttaaccaacc ttaaagcggg agattatttt
     4201 ttgacagtgc gagatgcttc aaattgtact gtaacttctg acgcctttac agttacgcaa
     4261 ccgcaagaac tgattgtttc ggctacatct accgctatta cttgttttgg agatgacaac
     4321 gcttccatta cactcaacgt tacgggcggt gttgaaccct attcggttca gtggagcaac
     4381 tatggcgagg gaatggtgca ggaaaatctt tctcccggaa catacgatat aacggttacc
     4441 gataataatg gatgccaaaa gtcattacag gtcatcattc ccgaaccatt ccgattctcc
     4501 gtaaaaccgg tggtaaaaca aataagttgt tatggagcca atgatggcag tatcaaactc
     4561 aattttgtag gaggaatgcc acccatttct tttgcatggg atgatgatcc aaatgccgga
     4621 gtagatagga accaattgaa accgggcgtt tatacggttc gcattcatga tgcccaacct
     4681 tgcgatatta tccgcagttt taccataata gagccgcaac cgctgactgt atcggctcaa
     4741 gttaaggatg ctaccgattg ccaaaacgta aatagcggtt ctattctgct tgcagttaca
     4801 ggaggaagac caccctacaa atatgaatgg tcaaatggaa tgacaacaca aaatattttg
     4861 aatgttcctc cgggaaatta ttcagtatta gtaactgatt cttttggatg taatgtcact
     4921 gttcagcatt ccattcaaag acaactacct ttatatgtgc aagtgaatag cgatattgaa
     4981 tttgattgct taactaaaaa tattatccaa gtcaaccagg caacagtcac tggaggagtt
     5041 ccaccctacc agatttcgtg gtcaagtgga acgattagtg gcgcaaacaa tgagatcatg
     5101 acgaccggtc aaaatggttt ggtttttgtt catgttactg acgcattgca atgcgaaatc
     5161 acaacatcta ttaacattca aattccggaa caaggtatca gctatcagct tctcgactgc
     5221 aatcagcgat cctatcagtt taatgcattg attcccatag agaaagcaga ctatatctat
     5281 tcatgggatt ttggcgatgg cggagtttct gatattaaaa atcctcaata taatttttca
     5341 aaaccgggta attatacggt aaaattgaaa gttcaaaacg cagaatgttc gctgtattat
     5401 gaaaagacaa tttctgttga agctcctccg gtgctttcac tcgataaaga acccatattc
     5461 tgtattggtg attctctgtt gcttcatgtt tcaggggcag actattatcg ctggaatgat
     5521 ggttctacag cagataattt attgattgtc aaaccgggga cgtattctgt tgaaggaact
     5581 tcaaaagccg gatgcagttc aattttgact ttcatggcca gttatttcga tttattccat
     5641 tataccatac aaaccgatca ggaaatggta accgatcgcc aaccaacgat agaattctgg
     5701 tcggaagaca catccaatgc tatttattac tgggattttg gtgatggata caaaactcag
     5761 ggtaatcacg tttctcatag ttacattatt tcaagagatg ggtattatga agttaattta
     5821 caagtgatca accccaatgg ttgccgggaa tatgctacaa aacgaatttg gattggagct
     5881 ccaactttgc ccaataccat tacgcccaat ggagatggaa aaaacgatct gttgctgaag
     5941 ggatttcata ttcaggtatt caacagaaac ggactactgc tgtttgatgg cagagaaggc
     6001 tgggatggaa catacaaagg gaaaccggtg tctgagggaa catactttta cgttttaatg
     6061 tatgctacac ccgaaggatt gaagtttgct tcaaattttg ttacagtaat tcgctaaaag
     6121 caatttaatg aaaatacgtt ttctgatagt gattttcttg tttgcagtaa ttgtttccat
     6181 gtttggacag tcaaatattc gcgtaaacaa ttattgggaa aacacttatt atatcaatcc
     6241 tgcttccatt cacgatcagt ataatggaga aataagcatg gctgccagaa atcaatggat
     6301 aaattttccg ggctcgccca ctacattttt cggacatggc accgtttttc tttccgattt
     6361 tcatacgcaa attggaatga aggcaatggc cgataaaatt ggttacacca atacctacga
     6421 tctcgctttt tcctatgctt accagttaac attaaatcct acatggaatc tgagtatggg
     6481 attgaccgtt aattatcaag gattgaacta cgacctgagt caggtcaatt taacgaatgg
     6541 tgatgaccct tcagcatacg aacaatttaa atttgaatcc aattttaatt ctaatctggg
     6601 tgtggagtta aataacaaat actggaaaat cgggttggca gggcaaaata ttttttcact
     6661 cttttactct ttcagcgaac tgttcaataa caccaatttt ttatatgcct cctacaaacc
     6721 ctatactcga aattttttca gtgtgggtgg aggaatgtgt ttgattcagt atggcaacat
     6781 gatgcaagca gaattgaact ttaatggctt cttcaaaatg acccctgaaa acaatgcttt
     6841 tcaactgggt ttattttatc ggacatggca cgaagctggt gccctttttg gcattgacct
     6901 gacgaattcc tttcggcttt cctacagcta cgactataat gccggtgcaa tcagacggaa
     6961 ctcttatggt acgcacgagt tgattttgac ttataaaatc aaaggagaac ctaaatgtat
     7021 caattgctgg tattgaaatt cgacatttta gtagtggact caatattgct ttataaaata
     7081 attcagactt tttcggatta aaaaatacag aaaagatgaa tatttttaat tttgactatc
     7141 aatttgtaga aaaaatttag ataattcaaa taaattaaat acatttgtag agaaaaattt
     7201 tttaattatt tatttctgag cttaaagatt aagttatggc acaacatcaa attgatagat
     7261 tggatcgtaa aattttgcaa ttgatttcac aagatgcccg tatccctttt ctcgaagttg
     7321 cacgcgaatg taatgtttcg ggggcagcaa ttcatcagcg catacaaaaa cttcgcaatc
     7381 tcggaataat taaaggttct gaatttgtga tagataccta taaggttgga tttcaaacct
     7441 gcgcttatat tgggattatt ttaagcgata ttaaaatgtt taagtctgtg gtagaggagt
     7501 tgaaaaaaat tccagaaatt gttgaatgcc attatgcaac aggaaaatat tccatgttta
     7561 ttaaaattta tgcaaaagac aaccgacatt tgttacagat tatactagag aaattaaccg
     7621 ttataccagg tgtggcgcat acagaaacct ttcagatttc tctggatgaa atttttcgtc
     7681 gtcagctttt tgttttcgat acggaaaatg aagtagaaaa tttagaggaa gagatataat
     7741 aatttctatt agtttaaatt aaaaaattcc ggttttctgg attgcaacac acaaattaca
     7801 aaattgttgc tgactaccaa tatataatat aaaaatcctg aaaattttga aaataaaata
     7861 gacctccaat cggaaaaagt gaaacaggca aaatggtgga cgctacaaca aattgaagaa
     7921 aatttgcagg aaggaatttt taccgaaaac tttctgatag aatttgattt gttgaaaaag
     7981 agtggagttt tggagaataa tatttgcgat tgcgcatgca aattgaaaga aaccattttc
     8041 aaagctcctc atctgtttcg gtaaggcaaa acctaaaaaa tttcttggtt attcaacttt
     8101 ttgcaatctt tggttggcta tttcgcaata gagtggattg atttcaaagc caataaaatt
     8161 tcttttcagc attttgcagg ccactgccgt cgttcccgaa cccatgaacg gatccagtac
     8221 caaatcgcct tcgttactgc taatttcaat gtacggaatg cacactttca atggtttttg
     8281 tgttcgatga atggttctct ctttccccat gcagataggt gcttcaataa aattgtgcat
     8341 ttcgttggtg ctcttgtaat tccatgtttt gggagtgcct tttgaaaagt gtacaatcag
     8401 ctccatgctg ttggcaaaca tttttcgggg atgtacggca gggaaaggat tggttttgtg
     8461 ccagtgaatg acttttctga attgaaagcc tgcccgttca accatacgat tgagaaaaga
     8521 cacaaaatcg tcgccgcacc ataaatatcc gctggcgccg ggtttgctca cacgataaca
     8581 ttcctttaat acttcttcca aaaattgcaa atactcttct tccgatttaa aatcatcaaa
     8641 ttcaaactcg gtaaccacat ccttatgatt tttaagtcct tttatgtttt gagcccggta
     8701 ttgataatat ggtggatcgg taaagataag atcaatacta tggtcgggta atttttttaa
     8761 accttccaaa cacgacaaag cataaatttg attcatttcc atgtggggaa aaagttattg
     8821 aatatttgtc tgttattttt tgttgatttt tccgaaatat gtttcgagca atttttttgc
     8881 tgcgacgaat gatgtaagtt cattggtaag aatcttgttt tcgatttcag gcaataattt
     8941 cctaatttct tcattttgat aaaaatgagc ctttagctgt tcgttgatgg tttcatacat
     9001 ccagtatttt gcctgatcgt ttcgtttata ttcgaaaaaa ccgttggctt tggtatattc
     9061 cacgtatctt tccaccatat tccacacgtc ttcgattccc gtcttttcaa tggccgaaca
     9121 agtcagcact tcgggtttcc atcccgattc agcgggagga aaaagttgaa gggcattttg
     9181 atgctgtatt tttgccagtt gtgctttttc gatattattt ccgtcgcatt tgttgataac
     9241 aattccatca gccatttcca taatgccgcg tttgatacct tgcaattcgt ctccggctcc
     9301 ggcaatttga agcagcagaa agaaatcgac catagaatga acggcggttt ccgattgtcc
     9361 cacgccgact gtttccacaa aaaccctgtc gaaaccggca gcttcgcaaa gaataatgct
     9421 ttcgcgcgtt ttgcgggcaa caccgcccaa cgttcctgca gaaggagaag ggcgaataaa
     9481 agcatttttt gaaacggaaa gttcttccat tcgcgttttg tctcccaaaa tgcttccttt
     9541 ggttctttcg ctgctggggt cgatagccaa aactgccagt ctgtgtcctt tttgaaccaa
     9601 atacatgccc aatgcttcga taaaggtact tttgccggct ccgggaactc cagttattcc
     9661 caatctgacc gatttcccgg aatatggcag acatttttcg attatttcct gagcaatatt
     9721 ttgatgttcg gtcaatgaac tttccaccag cgttacggct tggctcaaca aggaaatatc
     9781 gcccgataat atgcctttca catattctga tgcagaataa atttttcttt tttgtttaac
     9841 cttctgatag ggattgacaa taggtggctt atttacacca tcattgacac ttaaattttt
     9901 gaatgaagga tcattttcgg gatgtttttg atgaaagtgc atagttgaat ttttggaata
     9961 aaatagccag aatgtaacct ttttttacat cctggctcgt aattttctaa aaagaatttt
    10021 ttgtcagctg attgtccaat ttacgaccag gataacaaaa ctgttgttcg gatcgttggt
    10081 ttgcagggta atggtttttt tgtagttgcc cgatgccaag ttggcggtgt tgacggtaac
    10141 tttcagttct cctttgcttc caccttttat ggtttgaacg gaagaaacgg ttaactcctt
    10201 attgttgtta ataattcggt gtattttcaa ttcgtctttt ccgatatttt ttattaagaa
    10261 tttaccgctg atttttgaac cggccttaaa ggttcccagt gataaggtgg tggaaggaat
    10321 atccagaatt ggagcgtttt gcctttcgct gacggaaagt ttgctgaaat cttccactat
    10381 attggcataa agggtcagtt tgttgtcttc cgaaaatttc ttttgaccat tgacaatcag
    10441 ataaaaatcg tcggaaattg ggccccattg ctgacagttt tttgagttga gataaaaagt
    10501 aattttccct tcttcattgg gttgtgtaat atcgggaata acttctgaag taacatacga
    10561 aggcaagttt tcaaaagtaa ttcttacgtt ttgggaagtt gtatttttaa aagaaatact
    10621 tctgctttga atggttccct tgttgacatt gttcatttga acgattttgg ttttcagtct
    10681 gattccactc acagatatcg gataatcgga attttcatca gttttcggta tgacttctcc
    10741 tttaatttga agttgtacct gttcttctgt tgcattgctg aatacggtga tagttttgtt
    10801 aaaaattccc ggacgtccca aaggattata ggtaaccata atagaaccgg atttaccggg
    10861 ttcaataggt tcttttgtcc aattcggtgt ggtgcaaccg caagatgcct gtactcggct
    10921 gattacgagc ggtcctgttc ctgtattggt gaacttgaac tgatagctca cttttccgtc
    10981 ttcttcctta atttgaccaa agtcgtgaat tttttcctga aattgaatca ctgctttttg
    11041 cgcattggaa gcggaaacaa ataaaagcat cataaaaatg atggaaacgg tttttttcat
    11101 atatctattc ataacttgag aattgtttat tctgaaaata ttttgaaaat ttctctattc
    11161 agactacaaa aataggtaaa atgcgctttc tttgcacctg aattttgtct atatctgatt
    11221 aataatagtt aaaaaagaat tttcctttca cctcaaatat cttaaccagg aggagcatta
    11281 taattctctt agcattgttg gagaaacaaa tatttaaact tccggtttaa agtgataaac
    11341 ttctaaataa atggtttgtt taattgtttc aaaattattt tttgcaaatt cgtaatcgtt
    11401 gctcatcaac tgcatgggaa tggggaaacc tccgctttcg taaggtggct gaaggtagta
    11461 attctgtaat tcgacgaatt catgtcggtg ataaaattca attcttcttt ttgcagcatc
    11521 agtattgggc atttcaactt ccagtattac aggcaaattt actttttcaa gaaacgattt
    11581 caatgcctct gagcccaaat actggcttct caacttttct tcaatagcaa aatgttcgat
    11641 ataaataaat ttttcaaaat tccagtaatt gaaaattccc acaaagctgt ttttattcat
    11701 caatacgtaa gcatgaaagt ttggattgtt cagttccttt tccagattct gtaagcttct
    11761 gcgttcattt tcaggaaaag cagaagaata cagcctgtag aattgctgaa aaaacgcatc
    11821 agaagaggat tgcatccttt taaaatcaat catggcaaac aggtattggt atgtttgtta
    11881 ctacggtttt tttcaaggaa aaatgttgaa actttccggg ttcaaatagt ctttttcaat
    11941 atccagaaaa tatttgtaag tgcccacttt cagctcaatg caggcgtcgt aatcgcatac
    12001 gataatactt ttgggatgca gttgcagtgc gctcaccgtc cacatctgac tgataggttc
    12061 ttctactgca tggcgcagag cgcgagcctt gttgtgtcca ttaacgagaa tcaaaacttc
    12121 ccgagcatcc attatagttc ccacaccaac ggtaagagca gttttgggaa cctgattaat
    12181 atcgttattg aagaaacgcg aattggcaag tatggtgtcg gtagtcaatg ttttttgtcg
    12241 cgtacgcgat gaaagagatg agcccggctc gttaaatgcg atgtgtccgt ccggccctat
    12301 tcctcctaca aaaagctcaa ttcctcccac cgatttcatt ttttcttcgt aagctgcaca
    12361 ttctgctatc aaatcttcag cattgccatt gagaatattt acattttcgg gtttgatatc
    12421 aatatggctg aaaaagtttt tccacatgaa actgtaatag ctttcttcat gttcacgggg
    12481 taacccaaca tattcgtcca tgttgaaagt gataacattt tcgaaggaaa taatgccttt
    12541 tttgtttaat tcgattaaat aatggtacat gccgagaggc gaagatcccg ttggcaaacc
    12601 caaaacaaaa ggtttttgag cgcttggctg ggcatgaaga atttttgctg ccacgtaatt
    12661 ggctgcccac tgcgataaag tcgggtaatc tggctgaatg atgagtctca tgatgaaaaa
    12721 ggttttttat agttttatag ttttttacaa aagtacgatt atttctttcg ttttaaaaga
    12781 atattttaaa aaatattagt ccaacatttt ttgttgcaca aataaatttt atctcttctc
    12841 atttttgtcg ggatgatttg ttatttttgt tgtgagaaaa ccaaattttc agttattatg
    12901 aaaaatcacc tattgtctat tttattgttg gtagcctgcc atcaaagcat ttatgcatct
    12961 tcttatcaat cttttgttaa aaattctgaa gcgctcattc aacgtgtgtt gcccgaaaag
    13021 gcaaatcatt ttgtgattga acaaatttct tctgagcacg ggaaagatgt ttttgagatt
    13081 gctacatcaa aaaatggaca agttgtgttg cggggaaaca atgaactgtc tgttgctatg
    13141 gcttttaatc gttatttgcg ggatgttgcg catgtctctt acgactggtt ttccgtatca
    13201 cctttacaaa taaaaggaga gttgcctttg ccgatttcaa caataagaat ggcatgtaat
    13261 gcgcaagaac gattttttaa caatacttgt acttttggtt atacttttcc gttctggaat
    13321 tgggaacgat gggaaaaatt tatcgactgg ctggcaatga atggggtcaa ccggccatta
    13381 atgctggccg gacaggaaaa agtctggtta aatgtatggc aatcttttgg attgaaaaaa
    13441 gaagatattc aggcttattt ttccgggcct gcgcatttgc cgtggcaccg aatggcgaat
    13501 ttggataaat ggggcggccc cttgccggtt tcgtatatcg aaggtcagta tcggcttcag
    13561 aaaaaaattc tcgccagatg cagagaattg tctatgaaac ctgttttgcc ggcttttgcc
    13621 ggacatgttc ccgaacagct aaaacaaatt tatccggagg ctaacattca tcaaatttct
    13681 ccgggttggg gtggaatgga cgccgattat acaacctatt ttctcgatcc ttcagaaaaa
    13741 ctattttccg aaattcaata tcgttttctg aaagaacagg aaaaacttta tggcatttca
    13801 catcgctatt cggccgatcc tttcaacgaa attgacccac ccagctgggc tcctgaatat
    13861 attgcttctg tggcaaaaac aatttaccga tcgatgaaaa atgctgatcc agaagctatt
    13921 tggtatcaga tgtcgtggac tttttactac gacagcgaac actggacgca ggagcggctc
    13981 aaagctatgg ttgaaagtgt tccgaaaggg aaactgattt ttttggatta tgtggcagag
    14041 gaagaagaat tttacaagcg gacagaaaac ttttttggag cgccatttat ttggtgttat
    14101 ttgggaaatt ttggcgggaa tacccatttg gttgcgccca tgaaaaaagt ttccaaaaga
    14161 attgtagaag cactaaaagt ggcaaattgt cttggcgtgg gtgctacgct ggaagggctg
    14221 aatgtgaatc ccgaaattta tgaaatggta ttggaaattc cttgcagcaa tcaaatttct
    14281 tcgttcaatc tggacgaatg gataaaaaat tatgctgtca gaagagccgg acaggaagat
    14341 tcggctgtca ttgaagcatg gaagttgtta aaagaaaaag tcttggtgga cgaggctcaa
    14401 gtaattggga atcatagcgt tgtttttcag gttttgccgg tcatggattt aaaggaaagt
    14461 tcatggacta ccaatccatt tgttccttat gacaatcccg atttggcgaa agctttacaa
    14521 atcatgttgc aagcaaatga agaaagtcga aaaaatgatg cctattgttt tgatgtcgtt
    14581 aatttggcca ggcaagcact cggaaattat ggcagttgcc tttacgaaaa aatgatgact
    14641 gcttatcgag aacaaaatct ctctgatttt caaaaatatt ctcatcagtt tatttcactt
    14701 ggaatggaaa ttgacttgtt gttgggaaca agaaaggaat ttttacttgg cagttggctt
    14761 tcagatgcac gtcgctgggg aaaaacgaga gaggaaaaaa tctattacga aagaaatgcg
    14821 cgtgaaatta tcaccacatg gcataagtcc ggcggcgaat tggtagatta tgccaatcgc
    14881 caatggaatg gccttatgcg gagttattat ttgccacgtt gggaaaaatt tattgagctc
    14941 ttgcaaaagt ccttgataaa cggcacttct ttcgaacaag aagatttttc tgcctggtgt
    15001 atcgattttg agagaaaatg ggtgaatgga gaaattcaag agaatttttt ggaaaaaccc
    15061 agtggaaatg ctattgaaac ggctcaacgt ttgtttgaaa aatatagtac cgaaatgctg
    15121 aaataaaaat cgagtagtta gggggattac tctaccaccc tttcacatta ccatgcgtgt
    15181 tcttacacac agcggtttct tttaattgtt taacttctcc agctgaacac attgataaac
    15241 aagcaaacat actgtctgga ggatgagttt gggtactaca aatttgttct aaaaaataaa
    15301 actcattgaa atccagtatg ttacaatttt aatcgactga atttgactac caaactaacc
    15361 aatatcagtg tgtttttaac aaaactttaa cgttacgtta ttcaaatttg gaaagttgag
    15421 ctaaagaacc attgataata gattaaattt aaaatgaatg gttgtgtagg acagatttaa
    15481 tgcctaaaaa gtaagatagt attttcttac gaaggaatta agttttgaag agcgaaccat
    15541 atgtctttta ttttgaaata caaaagaata cggatatcaa attactgttt gcaaattatc
    15601 ctcttttcat ttcattgatt attttcatcg tatcggaagc aatcatgaat tcttcatctg
    15661 taggaattac ggcaactttc accctcgatt ctgcagaact tatgagtgct tctttgccac
    15721 gtgcttcagc attggctttt tcatcaacat ctatccccag ataagtcagt ccctttatta
    15781 tgttttgtct ggttttggcc tgattttctc caataccacc ggtaaaaacg attaaatcta
    15841 ctccattcaa ggcagcagca taagctccaa tatatttttt tatccggtac tcatacatgt
    15901 caagagcgag ttttgctctt tcattgccat ttgcaatggc ggtttcaata tcgcgcatat
    15961 cggatgaaat gcctgaaacg cccaatacac cgctatgttt gttgaaaagt gtagaaagtg
    16021 cagaaggccc aatcatttct ttttccatca gaaaagtggc aattcccaaa tccacatctc
    16081 cggagcgtgt acccatcatc aatccttcca atggtgtaaa tcccatcgaa gtatcaaccg
    16141 atttaccgtt ttctatggcg gtaatggagg ctccatttcc cagatgtacg gtgatgattt
    16201 tcgtttgtac gtaatccata ttcaagaatt cacaggcccg atgtgaaacg tagcggtggc
    16261 tagtaccgtg aaacccgtat cggcgaatac cgtatttggt ataaagagaa tatggaattg
    16321 catacatgta ggcatattcg ggcattgttt gatgaaaggc cgtgtcgaaa actgctactt
    16381 gtggaaccgt cggcaatagt tcgccgatag cattaatacc ggccaaattt ggtggattgt
    16441 gcaagggagc tatatcgatg cattcttcta tttttttgat tacttcgtca gtaatcagca
    16501 cacttgaatt gaatttttcg gcaccgtgaa ctactcgatg ccctacggca tctatttctt
    16561 ccagcgactt aatacaacca tgctttgcgc tggtaagcac tcccaatata tattcaatgg
    16621 ctgtctgatg ttccaatact tcaccttcca gaataattat ctgattgtct tgtcggatat
    16681 gtttcaaaag ggaacctttc atgcctatct tttccactcc acccgaacca agaactttgt
    16741 tattctccaa atcgagcaat cggtatttta cggaggaact tccgcaattc aacactaaaa
    16801 ttttcatttt ttatcctcct tattttataa cattaccaaa ttcatcatat tcgtaaaatc
    16861 cctttccttt ttttatgcca aaatgttttg cgcgagaaag acgcaacaag accggcgaag
    16921 gtttgtattt agaattgcca tattcctgat acaaattttc cataagtttg acaatttttt
    16981 cgattcccat ttggtcggct gttcggaata cacccaagcg atgaccaaaa ccaacggtaa
    17041 gtactttgtc tatgtcttca acgttggaaa tgccttccat taacatggca caggcttcgt
    17101 tgagctctat taaaaacaaa cgaatactca ccagccctgc cgattcggcg actgcaacat
    17161 attgatgatt gatcagacgg gcaaattgac aaattttttc gtacgtttcg tcggaagtgt
    17221 aaagccctct gacgatttca ataatatttg atccgggaac attggaaagg aaatgaagcc
    17281 ctatgcaacg ttccttatat tgcagttctg atgcaagttc cgtgataacc acagtggaaa
    17341 tattggaagc aatgatggct tgaggagaca ggactttttc gagttgaaga aaaacttctt
    17401 ttctttcttc aacgtgcctt tctcccgttt ggtcatcata gcgtactgct tcaatgacaa
    17461 aatcgcaacc ggtaaaatct ttatatttcg ttgttccctg tacgcggctg agagcaattt
    17521 ttttttcgtt ctccgtcaat ccccagttct gaattctttt attcagtttc ctttcaatcc
    17581 ggctgtaagc attggcaatt ttttcttctg taggttccag aaaaacaact tctattccgt
    17641 tgagcaaagc aatggttgca atagcacttc cttcgtttcc gcaaccgacc actccgattt
    17701 ttgaaaacaa agtacgtcgg cgactcctct tgctcagacc atatttttct atgggttcaa
    17761 taattggttg ggacatattt ttttattgta tggtaaaaac acttatttta tcgccagatt
    17821 ggaagtaatg gtaatcatgt gaaagatatc ctctaccgag cagccacgag aaagatcatt
    17881 gacaggtgct gccattcctt gcagtattgg gccaacagct tctgctccgc caaagcgttc
    17941 caccaatttg tatccaatgt ttcccgactg aagatcaggg aaaatcaata catttgcttt
    18001 tcctgctact gcactttcag gcgcttttcg atgggcaaca gaagggacga gagctgcatc
    18061 cgcctgaagt tctccgtcaa ttaaaagtgt cggttccatt tcacgggcaa gacgggtagc
    18121 tttcactacc ttatctacca actcatggct ggcactgcct ttggtggaaa aactcagcat
    18181 ggcaatgcgt ggttctattt ctccaatttg gcgagtcgtt ttagctgtac agaccgcaat
    18241 ttgagccaat tcctcagctg taggattggg atttactgca cagtcggcaa aaacaagcaa
    18301 tccgttttcg ccgtattgag gcgttttggt aaacatgaga atggcaccgg aaacaacctg
    18361 aatgccgggt aaagttttaa ttatttgaaa tgcagggcga agcacatccc ccgtaaaatt
    18421 ccgagctccg gcaagttccc cgtcagcatc tccgttttta atcatcagac aggcaaaata
    18481 aagcggattt ttcgtcaatt cttctgcttc ctgataggtt atgcctttgt ttttgcgaat
    18541 attgtacagc aaatcgatgt attctgcttt tcgaggactt gtttcgggat cgataatttc
    18601 tgcctcagca atatgattta aatgcaaatt cgatgctgtt tttagaatga catcttcttt
    18661 tcccagtaaa attaaatgag cagccttttc ttttaatatt atttcagctg cctgcaaggt
    18721 acgggtttca tctccttcgg gcaatacaat tcgtttaata tttgcttttg cacgctccaa
    18781 tattttgttc agtagatcca ttttttataa ttatttttca ctaatttgct gtaaacaata
    18841 cctatagctc ttttgctttt gagcaataca aaagtatcca aaataaattg aaattgcaat
    18901 gtagagatgg caaattttat tttttatttt tttcttagct gttacatatc gcgtccaacg
    18961 gatttttcat caaaaaatga aaaatttttg ttcattttaa ccatttatcc agccactcaa
    19021 agaaagtgcg ttgccacaaa attccgtttt gtggttgtaa aacccaatga ttttcatcag
    19081 ggaaaataag catctccgca ggaacaccct gcaaaactgc tgcattaaaa gctgccatag
    19141 cttgtgaagc gaggatgcga taatcttttt ctccgtgaat aatgagtatt ggagtatccc
    19201 atttgtcgac aaataaatgt ggcgaattgg cataagttcg acgaacgatg ggattttttc
    19261 tgtcccagta gggtccaccc aagtcccaat ttacaaacca gagttcatcc gtttcaagat
    19321 attgttgttc gagattgaac ataccatcat gagcgataaa ggccttgaat cgtttgttgt
    19381 gatgccctgc cagccaataa acggaaaatc ctccatagct ggcacccaca catccgagtt
    19441 tgtttttgtt tacaaaaggc tcttttgcca aggcgtcgat ggctgaaaga taatctttca
    19501 tattctgacc gccgtaatcg ccactgattt gctcgagcca agctttaccg aatccgggaa
    19561 cacctctccg atttggcgca acaataatgt agccattggc agccataagc tggaaattcc
    19621 agcggtacga ccaaaattgg ctcacagtgg actgaggccc gcctccgcaa aaaagtaaag
    19681 caggatattt ttttgcagga tcaaaatccg gcggataaat gacccaagtt tgcatttgct
    19741 ttttgtcggt agtggtaatc catcgttctt caacttttcc cattttaagt tttgacaata
    19801 gttccttgtt ctcaaaggac aattccgctt cctttcccgt aaccggatca ataaagtaaa
    19861 tctcgtcagg cttgctcatg gaatgtttca ctccgataaa gccatttctt gcctctgcta
    19921 caaattcata gtcgtgtact ccatgtgtga tgggagcaat tgattttgtt cgcaaatcgg
    19981 ttttgtagat ttgtgtaacg gcgtgccaac aactgacaaa ataaatagaa cggctgtcgg
    20041 cactccaaga aattgcattg caattctgat caaaatcttc cgaaagatcc aatttttctc
    20101 cgctttgaac attcattacg aataaacggt ttttatccga ttcgtatcca tcgcgttcca
    20161 tgctttccca agccatccat ttcccgtcgg gagaaaactt tggattgatg tcgtaaccct
    20221 gcattccttc tgttttattt tcggttttac cattttcgag atagtagaaa taaatatccg
    20281 aattggtgga aagggcatat tctttcccgg ttttttttcg gcaggtgtaa gcaataattt
    20341 tcccgtcagg actccagtcg agttgttcaa taccaccaaa aggtttcatg ggacattcat
    20401 aaggttctcc tttcatgata tcttgctcat ttttcagcgt attgtcttta aagtcggcaa
    20461 taaaaggatg aggaatactc tctacccatt cgtcccaatg cttgtacatt aaatcggtaa
    20521 caattcgccc tgttgctttt ggcaaatcgg gatatttgtc ggcagcagtt ttaccgtatt
    20581 tgacatcggc aataaaaagc actttcgatt cgtcgggaga aaaaagaaat ccattgatgc
    20641 ctccatcgcg gttggaaatt tgtttgcgct cacttccatc gattttcatt gtccataact
    20701 ggttgcttcc actttcggaa gagaggaaag caatgtgttc gccatctttc atccatcggg
    20761 cctcggcttc ttttgtggct gtatgagtga tttgatgctt gtttgttcca tcaacgttca
    20821 taatgaaaag ttcagtattt cctttatttt cgggaatact gtaataggta acactataaa
    20881 gtattttggt tcgatcagga gaaatttgta ctccgccaac tcttccaaaa gaccataaaa
    20941 cttcgggcgt catgcgtcca ttttcgatat tgggaatctg tttcccgatt acggaatgat
    21001 tttctttttc agtgcatcca gcacctaaaa cggctattgc cataagaatc gttttaaatt
    21061 tcatttgtag ctttcaattg taatttttaa gcgtaagtct aaatattttg aacaaaatcc
    21121 tgtctcaaat gcaaaagtaa ataatttccg aatatttata aatgttctga aagattttct
    21181 ggtaaactct gcaattttga aaaaaatatg atgagttttg gtaaatctca aaaaaaagcg
    21241 tacttttgca ctccaatcaa tcgtaatctc tgacagaata aaagaagtct gtatatattg
    21301 cgtatttact taaaaaatca agtgagatgg acttattaaa aattgcagaa caatcttttg
    21361 agcaggaagt aaaaacttat cctgagttta gaagtggtga taccatcaca gtagcttatc
    21421 gaattaagga aggaaacaag gaaagaatcc aggaatttaa gggtgatgtt ataaaaattt
    21481 cgggtcatgg aggaaagaag cattttactg ttcgtaaaat gtcgggaaat gtaggtgtag
    21541 aacgaatttt tcctatggat tctccgttta tcgaaagtat tacggtcaac aagcatggta
    21601 aagtgcgtcg agccaagctg tattatttga gaaatctgac cggcaagaaa gccaaaatta
    21661 aggaaagaag agtttgacat tggcttttcg gcaaagaaat taaaaacagg agaagtttgc
    21721 ttcattgcag atttctcttt ttttgtttct tatcgtcatt tttcaaccga aaaaagtttt
    21781 ttcttttttt ccccacaaca gcatgacaat tccacataca atcggaaaaa cggctattga
    21841 aatcagcaac atatcgaaat gcataaccag cagattttct ataaaattca acaatatccc
    21901 ggtatggata taatggtaca gcaaaaacga aatgcctgtt gctccaatta gccatacaat
    21961 ccaggaatga tctttctctg ttggcaaccg gcgcatgact tttaaggaaa aaccattgtc
    22021 gggtatttcc gttttatggg ttttgaaaaa ttcctttaat ttttcctctg tttgttcatt
    22081 ttttgtcata accttcctta tttagataat ccatcaattt gttttttccc tttagcaggt
    22141 aggtttttac cgttccttcc ggacaattca tgatttttgc aatttctttc tgagttttgt
    22201 cttccatata atgcaaaatg agcactgttt gttcttcttt ttttaagtat tgcaaagcgc
    22261 tgtaaaagtc caacttttca gcagaaaacg cattgtcgga ctgatattga aggtcaattt
    22321 ccctaacgtc gagttcggaa aattgttttt gagtgcgcat tgagtcctaa aacacattgt
    22381 aagcgatgcg ataaagccat gtggaaaagt tggacaaagc ttgaaacgag tgaatattca
    22441 caaatgcctt gataaatgtt tcttgtgcca aatcgtcgct caatgaggca tcgcccattg
    22501 tcagattcaa aaagaaccgg cgaatgggcg actgatattt gccaacgagt ttttcaaaag
    22561 ctttgttgtc gccaagcaag gtgaattgtg ttatccaatg cagatcatcc ctgttgctca
    22621 ttctgctcaa ttggttgttt ctcttttgac cgatcgtaaa aatgcactac taaatatcca
    22681 atgcccacaa aagttggaac aattcccaca atcagcccat cattattgtg attgatgata
    22741 aagtaaataa gcaaggcaag tcccactgca gcccataata cgcctttttt taagttggag
    22801 ttcggcttat cctttttatc tttctggaaa aaatcttccg ggatcggctg tcccatttcc
    22861 aacgccttca tgtacagttc aaattttcgt ttcgactcct tgttttttgc agcataaata
    22921 gtccatatca gaataatgac aaaaaggaaa ggtgcaacca caatcatgaa aatctggaat
    22981 cctgtaagtg gcatgtcttc tcgtgcattg ccttcaattt ttttcctttc gttttccagt
    23041 tccgcttttt tcagttccag caattgttcg ggcgaaagtt tattgagcaa gatggaatct
    23101 ttctcccgct gggtcagatt ttccaaagca gccggcaatg acggatggga actttcatca
    23161 gatttgttct gcgcaaaacc gataccggaa aacagtatca gcgaaaacac caataatacg
    23221 agctttttca taatgtttga aattttagaa gtaaatataa tgtttgttta tcagttagac
    23281 gtaaaggaaa cagtgttttg atttgcaaag caatggaatt aacttttatt agttttcatc
    23341 ttttttttta tattttaaca aactagagga aatttttctt tgttggtgaa cttgaaccaa
    23401 atagtttttt atctgtttat ataaagaaat tttaattcag aacaaaccga aatgaaaaca
    23461 gaaaaatttg atgtggtggg aatgagttgc gctgcctgtg cagcccacat tgaaaaagag
    23521 gtcggaaaac tggaaggtgt taaaaaagtt aatgtcaatc tgctgaccaa cagtatggta
    23581 gtagactttg aggaatcgaa cgtagatact gataaaattg aagaagccgt tgtagaagcc
    23641 ggatacgaag cacatgtaaa atcatctgca gagcaggctg cgagtcggga aacgaaaaaa
    23701 atagattttg ttcaaaaaga acaacaggaa atgcagaaac gttggtggat tactttcggt
    23761 ttctggttac cgttactgta tttgtctatg ggttccatgc tgggttggta tataccggct
    23821 tttttcaggg gagaagaaaa tgcaatggtt tttgctttta cgcagttttt gttgactctt
    23881 cccattgcaa tgtcgaacga aaagtatttc agaaacggtt tcaaaatgct ttttaaggct
    23941 gctccaaata tggattcact gattgccata ggttcttcgg cggccatttt atatggaatt
    24001 tacggattgt acaaaatagc ttacgggctt ggtcatgcac aaccggagtt ggtgcatcag
    24061 tttgctcacg atatgtattt tgagtcggga gcaaccattt tgactttgat tacgcttgga
    24121 aaatatctcg aatcgcgatc aaaaagtcaa acatctcggg ctatcacgca attgatggat
    24181 ttgtctccaa aaacggcaac tgttattcga gacgggacag aagtagaaat tcctgcagaa
    24241 gaagttagag taggggacat aattgttgtt cgccccggtc agcgaattcc tgtggatggc
    24301 gttgtggaaa gcggaagttc tacagtggat gaatcggcac taaccggaga aagtatcccg
    24361 gtactgaaac aaagcggcga ccgagtattg tctgccagca tcaacaaatc cggatatatc
    24421 acgctcaaag caacgcaagt gggtaatgat accactcttt cgcatattat cagcctcgtg
    24481 gaagaagctt cttcctcaaa agctcccatt tctaagttgg ccgacagaat aagccgtatt
    24541 tttgtacctg tggttatttt tattgctttg acggctggca ttgtctggtt ttctttaggg
    24601 tattctttcg atttttcctt gtcagttgcc atttccgttc tggttatttc ctgtccgtgt
    24661 gctctgggac ttgcaacgcc ggttgccatt atggtgggaa cgggaaaagg agctgaacac
    24721 ggaattttga taaaatcggc tgcttcgctg gagatggcac acaaaattga tacggtagtg
    24781 ctggataaaa ccggaacgtt gacaatggga aaacctcaac ttaccgacat cattccttca
    24841 ccaaacatta cggaaaatga attgcttcaa atagccgctt cgctggagaa actttctgaa
    24901 catccattgg cagaagctgt tctggaagaa acagacaaaa ggaagctgga aactttttcg
    24961 gtgaaaaatt tcacttctct gcccggaaaa ggaattgttg ctgaagccgg agaaaattat
    25021 tttctgatag gaaacagaca attaatggaa gagcagaaaa tagatgtaca gaatttcgat
    25081 aaaattgcca ctcaactggc tgaagaagga aaaacaccct tgtttgtggc ttccagggaa
    25141 aaagtaatag gagtgattgc tgctgctgat ccattgaaac caaccagcaa ggaagccgtg
    25201 aaccaattcc gttctttggg actggaggtt gtcatgctga caggtgatca caaaaaaacg
    25261 gcggctgctg ttcagcgtaa attgggaatt tcaaccgttt tttcagaagt gttgcctcag
    25321 gataaagata aagtaatagt aaatctgcaa aaaggaggga aaaaggtggc aatggtggga
    25381 gatggcatca atgatgcacc ggctttgatg cgtgcggatc tgggaattgc cattggtgcc
    25441 ggtaccgata ttgctatcga atcggcagat gttgtgttga tgcgcaatga ccttatggat
    25501 gctgtttcgg ctattcgact gagtaatgcc gtgatacgga acattaagga aaatctcttt
    25561 tgggcatttt tctataatat tattggtata cctttagctg caggtatttt ttatccgctg
    25621 ttgggctgga aactcagtcc catatttgcc gcagcagcca tgagtttcag ttctgttacg
    25681 gtggtgctga atgcacttcg acttcgcttt tttaaacctt cggaggtgaa atcttaaagt
    25741 tgaaaagtct caaaaagaat ttcagataat ttatagtttg ttgttgagta tagatgagag
    25801 ttatttcaat tttgcaaatt gaaaaatatc caataatcga tttataggta tgtttacaaa
    25861 ctatttgctg tgttgaatga caggatgact atcagactat ttttgaaatc tttaattccg
    25921 aacagaaatt tttttatagt aagatatcga ttatagttgc ttctcattga gattcattat
    25981 ctttgcagac gacaaacaaa tgcaaaagag accaaaatgg aaaatcagat caatgggcct
    26041 aaaaagcaat acttcgaacc catgcacaca tgcgaacata ttctcaacca gactatgatt
    26101 cgcatgtttg gttgtgggcg ctctcaaaat gcccacatcg aacggaaaaa gagcaagtgt
    26161 gattatcttt tgccgcaggc gcctacagag gaacaaattc gggaactttc cgaccgggta
    26221 aatgccgtta tcgacagtca tttgccggta accatcgaat ttctgaaacg ggaagaagct
    26281 tccggtgtag tagatttgtc gaaactcccg gatgacgctt ccgacgtgtt gcggattgtc
    26341 agagttggcg attatgatgc ctgcgcctgc attggccagc atgtgaacaa tacttccgaa
    26401 atcggacatt ttgaaattat cagccacgat tatgaagatg gcaggtggcg cgtacgtttt
    26461 cgtctgatcg actaacaaat tttttcgtta tgttcaggaa agcttttaaa ttcattaaaa
    26521 actttatcgt tttattcttt ctcatcagca ttggatgggt gattatctat cggtttgtcc
    26581 cggtaatggt tacgccgctg
//
LOCUS       DAOSCH010000016        25288 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1411_length_25288_cov_6.800856, whole genome shotgun sequence.
ACCESSION   DAOSCH010000016 DAOSCH010000000
VERSION     DAOSCH010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 25288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25288
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1411_length_25288_cov_6.800856"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="PLZ54_02550"
     CDS             complement(<1..921)
                     /locus_tag="PLZ54_02550"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HOJ65850.1"
                     /translation="MKYLIILGDGMADEPIESLGNRTPLQVANKPNIDKIAELGRSGM
                     LDTIPEGFSPGSEIANLSVMGYDVAKVFEGRGSLEAASMGVPIEKGEMAMRCNLICIE
                     NGKIKNHSAGHISNEEAAELIKFLNEKLGNEIISFYHGVSYRHLLKMKGGNKNLKCTP
                     PHDVPGTPFKEVMIQPEVPEAEATARLLNELTLQSQSLLENHPVNLKRAAEGKDKANS
                     IWLWSPGYKPEMKTLMELYNLKSGSVISAVDLIKGIGVYAGLKVINVEGATGLYNTNY
                     EGKAQAAINALRTDEFVYLHIEASDEAGHEG"
     gene            complement(1117..2145)
                     /locus_tag="PLZ54_02555"
     CDS             complement(1117..2145)
                     /locus_tag="PLZ54_02555"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOJ65851.1"
                     /translation="MISKIIAEDLINKVYSSLQDAEKIAIVVHVNPDGDAMGAALGLW
                     HYLMTIEKEPTVISPTAYPNFLSWMPGANEVLIYENDKEKADNVLASAELIFTLDFNS
                     LHRLEEMAEAVASSPAPKILVDHHLHPESFAKITISYPEISSTSEMIFRLICRMGDFA
                     NINLACAECIYTGMMTDTGSFTYNSNDPEIYIIISELIKIGIDKDEIYRKVFNTYSEN
                     RLRLMGFCLYEKMQTFPEFRTAIISLTQEELSQFNYVNGDAEGFVNIPLSVKGIDFSV
                     FLREEKNRIKISLRSQGKFPANKVAADLFGGGGHLNAAGAESYASMQETIHTLLNALP
                     DYQVFFEE"
     gene            complement(2296..4119)
                     /locus_tag="PLZ54_02560"
     CDS             complement(2296..4119)
                     /locus_tag="PLZ54_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOJ65852.1"
                     /translation="MANIPDSLKENADAILRYSNVSYEIKENKLIKQKSFAITLMNDN
                     AKENAYLIIPYDSYTNIKKIEGYIYNANGIKTEKLSKENIKDYAAYPSFTLFSDNRIK
                     VVGFSAKNYPVTIEYIYELESKGLLMPESWVPINDYRQSLEKAQLQIIVPDSIIQYKY
                     QNIRPTFTKETVNNNIIYSWKINGIKAKESEPFSLPFQLIMPKIFVNASKTKYFEYPV
                     EFNDWKSYGKMVFNFLTGREIVPTKSVEEINSLCKNANTELEKVKIVYAYVQNKVRYV
                     NVTKGIGGFQPLPAAEVDKYGYGDCKALSNYTRALLNAVGIKSYYAEIGNGNNKRIQF
                     PDFASVDQTNHVILFVPLKSDSLWLECTSNTIPFGYIGSGNCNRQALVVTEEGGKLID
                     TPSYNEFDNLLSISGEIFVEETGNAHLKIKKTYSNELFDEVMPFLSLSKQETEKYINK
                     SIPINGFHLTGYSFINKSSYKNIKGELAFEGDILQYAELMGKRIALKPNIISRSYFGL
                     NKKRNNNVYFSQSFTQTDTIAIHVPSGYKLEFEYPEISFSCPAGYYSTKISQKKADSF
                     LMIRKLTIKKGIYGKENYPEIYDFFKNSALADNKSCIFTKL"
     gene            complement(4191..6233)
                     /locus_tag="PLZ54_02565"
     CDS             complement(4191..6233)
                     /locus_tag="PLZ54_02565"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOJ65853.1"
                     /translation="MKKSLLPAFMLAFVTCCGIFAQNSPSKFGNVGKEELEQNVCPID
                     SNAHAYFIFNCGLSRFTYPQTHVREDDAGENQGFVLETTKHFRIKILDESAFDWANIK
                     IPLYVSGKKKEKLSSFKASTFNINNGKVEETKFNKGNLIFESTSENWETAKAPLPNVK
                     KGSIIEVVYTVTSVFFYNLDKWNFQYSIPVLFSDFEVEIPEYFNFHQNIAGYYAIDRK
                     TERKNESLDLTYHWQDFNGRYSNTQRINFYVDISEYIGRNIPAFKEDSYLKTPKNYLS
                     AVEFELAYTKFPNSTLKYYTTSWENVIEQYLKYNDLENSIKVGFLDDIAKEINNKAKN
                     PYSKMELAFYNIANHYKWNEKNSIFTSKGFKDSYTNATGNCADINLCLVGLLQKLGLE
                     AYPVILSTQNNGFIKQTHPSLSDFNYLVALAKIDGNDYLMDATEPLSIINQLPVRCLN
                     DKGLIVKKGQANWVDMLKDASYKSQIILTYELDENLEAKGTYSSILNNYAALEIRKNI
                     KSYTSNDEYFKEIAKNNPGIETNEYQIEGLDSLNIPVQMKFDFSYNNAVEQAGDLIML
                     KPLLFEAMKQSPFKLSERTYPVEFNYQNKDKTTCQIKIPNGYAVESLPSSINLTLPDK
                     SAQFLFFTSIKDDYILIVSNYTRNKLLYLPEEYEALKEFFRNMVVKQNEKIVLKKL"
     gene            complement(6543..8615)
                     /locus_tag="PLZ54_02570"
     CDS             complement(6543..8615)
                     /locus_tag="PLZ54_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOJ65854.1"
                     /translation="MNFLQRTPFIRLLLAYICGIVVYHYAELFPATLWSLFFLSIALL
                     LIPFFIRETRLQFNFRWLFGTGVFLFLFITGYIFSFERDKASKFYPLNQKGIYVVELT
                     SAPVEKAKSVFYTAEIKKFLHNRHWESGHGKAYIYLQKDSLSGNLVFGDRLMLETEFK
                     SPEKLLNPDGFDYATYLKRQGVGATAYIASQHWQKIGHNQAFSIRRMADQCRNYLLGI
                     YRKFNIQGNEFGVLAALTLGYTEDLQPDLRKSFSASGATHILSVSGLHVGTVYAVLLF
                     IFGFLNHNQRQKISKIIIITLCMWGYAFITGLSPSVVRATLMFTFVALANCLERKSQI
                     YNTIFMSAFFMLLVNPNFLFDIGFQLSYSAVLSIIFFQPPLTKLLPTNHRFLKWGRDL
                     LLVSVAAQIGTAPFTLYYFQQFPNYFLLTNLLVLPLSTLVIYLAMGLLAVSFIPYLST
                     AVAFLLKISVGLLNFCIVQIEHLPYSLSYISLDIKQTILIFMVIFSLSAYYYNKKFVP
                     LALGLISLLLVCVVDLQIKYNTLRNQKIIVFAGQKSTHVNFIDHGKNYVYTTDSVELE
                     KIASNYWRKNKLQNPEYLSNSNWFENGFASFYGIKILILTDEQLKRKVTETPLNIDLL
                     IIGNHLKPRMDQILSCVQPQKIIVDKSISKWYTNDIRQQCEKHGIEFYSVADQGAYIL
                     DIPNKKSL"
     gene            complement(8707..9360)
                     /gene="rpe"
                     /locus_tag="PLZ54_02575"
     CDS             complement(8707..9360)
                     /gene="rpe"
                     /locus_tag="PLZ54_02575"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOJ65855.1"
                     /translation="MNRKIISPSILAADFSNLQAVCELINQSEAQWIHLDVMDGIFVN
                     NISFGFPVLEAIRKHCRKMMDVHIMIVHPEKYVTRFAKAGAEMLTFHFEAAENPQKVI
                     DQIHEEGMKAGITINPDVPVKVLEPFLNQVDMVLLMSVFAGYGGQPFIEETYERMDEL
                     KKMMELQKATCLLEVDGGVNLENAPKLFSHGANVLVAGSSIFKAENPLEIIQQMKNC"
     gene            complement(9835..10299)
                     /locus_tag="PLZ54_02580"
     CDS             complement(9835..10299)
                     /locus_tag="PLZ54_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ65856.1"
                     /translation="MALPKVFRSGSNRTPSLPSLIDRFFDTDLLDWDAFNFAGENMTL
                     PAVNVKETKDEYCIEVAAPGLNKEDFKVSYDDGMLTISSEKQQEKEEKEGEKVTRREF
                     SYESFQRSFTVPEEVVNADKISAKYENGILYVTLPKHEKAKKEKNVKEIKIS"
     gene            complement(10923..12209)
                     /locus_tag="PLZ54_02585"
     CDS             complement(10923..12209)
                     /locus_tag="PLZ54_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HOJ65857.1"
                     /translation="MIYFEKGSATTDLTPEDLKNGLYEALEKLGKRQKVLAIPPDFTR
                     YPSHAGELTEFAWQYYGEKLTDVLPALGTHTGMTDEQIATMFGQVPKTIFRVHDWRHD
                     VITLGYVPGSYVSEVTEGALSFDWPAQVNKLLVEGNFDLILSIGQVVPHEVVGMANYN
                     KNIFVGTGGQEGINKSHFIGAVYGMEKMMGRANTPVRKVFNYASEHFAAHLPIVYVQT
                     VVALNKETGKLQTYGLFIGDDFEVFDKAAGLSMQVNFEMVEKPLNKVLVWLDPAEFKS
                     TWLGNKAIYRTRMAIADGGELIVLAPALREFGEDKEIDRLIRKYGYFGTPKTLKATNE
                     NEELQQNLSAAAHLIHGSSEGRFTITYCPGKNPENLTKEEIESVGFRWGDIDEVTAKY
                     HVSTLKEGWNVMPDGEEIFYISNPALGLWAHKDRFR"
     gene            complement(12324..13778)
                     /gene="gnd"
                     /locus_tag="PLZ54_02590"
     CDS             complement(12324..13778)
                     /gene="gnd"
                     /locus_tag="PLZ54_02590"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008158292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HOJ65858.1"
                     /translation="MKKLADIGLIGLAVMGENLVLNMESKGFTVAVYNRTVEKVDKFI
                     NGRGAGKNFIGAHSLKELVDSLERPRKVMMLVKAGQPVDDLIEQLIPLLDKGDIIIDG
                     GNSHFPDTIRRTKYVESKGLLYIGTGVSGGEEGALKGPSMMPGGSPAAWPYVKDIFQA
                     VAAKVEDGTPCCDWVGENGAGHFVKMVHNGIEYGDMQVINEAYFLMKELLGMIDDEQH
                     EVFKKWNTGKLDSYLIEITRDILAFKDEDGTPLVEKILDTAGQKGTGKWTVDAALDLG
                     IPLTLIGESVFSRCLSAQKDLRVEASKAISGPKKAFKGDRQQFINDLENAIYASKIVS
                     YAQGYDLMMQAAKEYGWNLNYGGIALMWRGGCIIRSVFLGDIKKAFDQNPNLRHLLLD
                     PFFKEQVESAQEGWRRVCAVAMENGIPVPAMASALSYFDGFRTERLPANLLQAQRDYF
                     GAHTYERIDRPRGEFFHTNWTGRGGDTASTTYNV"
     gene            complement(13849..14928)
                     /locus_tag="PLZ54_02595"
     CDS             complement(13849..14928)
                     /locus_tag="PLZ54_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOJ65859.1"
                     /translation="MSLQLRKNAKYALLTPTSMGVRITPVNGQPVHSSQLFEMQATSA
                     ETNVASISSFLGLPVKVLTTFVKDSPIASFIKSDLHRRNIDFEGPEIPQGGPWGYRHQ
                     FNIADSGYGLRGPRVLNDRAGEVGRTLNVKDFDLERIFSKEGVKIIHLSGLIAALSPE
                     TSNFCLEIARYAKKEGTLISFDLNYRATFWKGREAELRTTFSEIASLSDILIGNEEDF
                     QLCLGIKGPAEGGKGLENAIDSFKEMIMNAQVTFPNVSVFGNTLREVISANEHLWGAI
                     VYNGKDWQVIKPRPIYVLDRIGGGDGFVGGLLYGILKEWNIEKCTQFAWASGALATTF
                     LTDYAQPADEEQIWSIWEGNARVKR"
     gene            complement(15335..16147)
                     /locus_tag="PLZ54_02600"
     CDS             complement(15335..16147)
                     /locus_tag="PLZ54_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ65860.1"
                     /translation="MYELFDIKDKVIAFTGGTGVLGESMVKYLAAHGAKIAVLARSKE
                     KGDKLVEEVKGKGGEAMFLVTDVTNETILRQNVEDILAKYGKVDVLINAAGGNMPGAT
                     IGPNNTIFDLKLDDFRKVVDLNLMGSVIPTIVFAECMVKANKGNIINFSSASALRPLT
                     RVAGYGAAKAAVTNFTKYMAGELATKFGENFRVNAICPGFFITEQNRTLLINPDGSYT
                     ERGKAVIAHTPFRRFGVPEDLLGTMHYLVSDASKFVTGTVAIVDGGFDAFCI"
     gene            complement(16212..17459)
                     /locus_tag="PLZ54_02605"
     CDS             complement(16212..17459)
                     /locus_tag="PLZ54_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HOJ65861.1"
                     /translation="MKLGKYSFGVGDRFSHQGKAQLKALMKANEKGLNIIPVWNKSNR
                     EHTYVHSQPKDTRVEADNAVKALNYKGPYFVDADHINLNTVAPFVQVSDFFTLDVAAY
                     IGKESTKEEVNQFIDSCKKYMGQLEIPGLKEPLNVSKDLLEKITNKFLAATQQASEIY
                     AYLKKEKGADNFITEVSMDEVESPQTPVELFFILKMLADKGVPVQTIAPKFTGRFNKG
                     VDYAGDLEQFAKEFEEDVLVIDFAVKEFGLPEELKLSVHSGSDKFSIYPVMSRIIKKH
                     DKGLHLKTAGTTWLEEVIGLAKAGGDGLMAAKKIYANSYNRKDELCAPYADVIDIDGS
                     KLPSVEEVNAWGSEKFVNTLRHDQTCPDYNPNFRQLIHVAYKVAAEMGDEYISLLEKY
                     EEIIGQCVEENIYDRHLKRLFDL"
     gene            complement(17487..18893)
                     /gene="uxaC"
                     /locus_tag="PLZ54_02610"
     CDS             complement(17487..18893)
                     /gene="uxaC"
                     /locus_tag="PLZ54_02610"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HOJ65862.1"
                     /translation="MQSFINDDFLLQSDAAKELYHQHAEHQPIIDYHCHLNPEYIAND
                     RQFDNLGQIWLEGDHYKWRAMRSNGIDEKYCTGKDTSDWEKFEKWAETVPYTMRNPLY
                     HWTHLELKRAFGVDKLLNPATAKEIFDECTAKLRTKEYSARGLMKKFNVEVVCTTDDP
                     VDSLNYHIALKQEGFGIKVLPAWRPDKAMAVENPVEYRKYVEKLSEVSGVTIRKFDDL
                     LQALRVRHDFFADLGCKLSDHGIEEFYAEKWSKSEIEVIFNKVYGGKELTQEEIRKFK
                     SAMLYEGAVMDWEKGWTQQFHYGAIRNNNSRMMKQLGPDTGFDSIGDFTVAKAMSAFF
                     DSLDVSGKLTKTIIYNLNPRDNDLVATMIGNFQDGSVPGKMQFGAAWWFLDQKSGIEA
                     QLNSLSNLGLLSRFVGMLTDSRSFLSYPRHEYFRRILCNLIGNDVENGLLPASEMDFL
                     GKMVENISYFNAKNFFEF"
     gene            complement(19057..20268)
                     /locus_tag="PLZ54_02615"
     CDS             complement(19057..20268)
                     /locus_tag="PLZ54_02615"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HOJ65863.1"
                     /translation="MFKISRSISLIVFALLIHNVYSQSVTVKNQSNVLLKDFTVEILV
                     NKLPLTIGNYYVVSKEGVQLPVEIVSDINAEQIAVFSIERLQPNAILKFNFYRGQAEN
                     YPKRTYAEIAHKIGGQFVNGKYQGEFSWVKPNFIRLPGNFKDHSYFIKYEGAGWESDK
                     VAYRFYLDQRNALDAFGKKTPGIVLPAVGVDGYDNYHKMAVWGMDDMEVGKSLGIGSI
                     AYWDGKKAFRVEKRDSLVCCVISDGKVRSQIKTVYYGWEAANKVNLTSLITIDAGHRA
                     SHMELKIDGKLDNIATGIIKLKNTEIIVPPANDGEWTYYATFGCQSMNNDNMGLAIFY
                     RKRQLQTLTEDELNHVIVLTPDHGYVEYYFMATWEKEWQPIQDKASFINAIDDELNCL
                     NQKVSISINKK"
     gene            complement(20282..22168)
                     /locus_tag="PLZ54_02620"
     CDS             complement(20282..22168)
                     /locus_tag="PLZ54_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOJ65864.1"
                     /translation="MKFSHSIFLFGAIVLCFPVKAQTVMINGVPRDTSYTVYSTYIKL
                     KKQYPFIRPAEARMSDNLKSYENIAYKFISPERKLLLNIYRLDNNEVLPAIMMIHGGG
                     WNSGSPSLQKAMAVKLAQKGFVCITVEYRLIPEALFPAGEEDLEDAVRWIADNAETYG
                     INRDKIAVSGCSAGGQLAALIGTKNKDKLIKTVVNIDGISSFIDKATIDRAQKARNIG
                     DKMPVDALWLGGTFAERPENWKAASAVTWVNQNSAPVCFINSSIPRFHNGRDEHIRML
                     DSLGIYSEVHAFDDCPHSFWHFHPWQLSTVQYVANFLNRILYNTPIAVDHSKYDLIVA
                     QDGTGDFRTVQEAINAVPDFRKRKTSIFIRNGFYREKLIIPETKDSLTLIGEDRNKTI
                     LSYNNFASKPSGFGDQLGTSGSASVYICSPNFTAENLTFENAAGPIGQAVAAIVRSDK
                     ARFLNCNFWGFQDTLYPHKAGSRQYYKNCYIEGTVDFIFGFSTAYFDSCELYCKESGF
                     TTAAATPQENNYGFVFYRCQIHGENPASFYLGRPWRPYAKVAFIECDMTNVIKPEGWD
                     NWGKVSNEKTAQFSEYQNSGEGGNLTNRRVKWSKTLSSRQVKNFDKEIVLGKDFFEKN
                     RENH"
     gene            complement(22261..24768)
                     /locus_tag="PLZ54_02625"
     CDS             complement(22261..24768)
                     /locus_tag="PLZ54_02625"
                     /inference="COORDINATES: protein motif:HMM:NF012754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ65865.1"
                     /translation="MNKTVILFLHLMFFFSLSAYSQSIDIFEANGWLESACLKWHSSG
                     SVDSYNVYYTGGGITNKKIDSPLIRKYADYWRADIVGLAEGEYSFTVKPVVNGTEAEG
                     ASVSAIMVKAHDRSGFAFQGSRVPGAYKSDGTLKDNAVILYITESNKNSVSMTVTGAT
                     TNPCVGLQSILDGFKKGKDTRPLSVRLIGQITDLDYMLNGDIVIENNNNASSYITFEG
                     IGDDAVADGWGLRIKNASNIEVRNLGFMNCDSDEGDNVSLQQNNDYIWVHNCDFFYGE
                     AGSDADQIKGDGALDCKKSTYVTFSYNHFWDCGKCNLLGLGEGTTSGLYATYHHNWYD
                     HSDSRHPRVRYYSTHVYNNYYDGNAKYGVGSTTGASVFVEGNYFRNCKYPMLISKQGT
                     DIYQSSGTFSGEDGGIIKAFNNYMTGQNRFVAYGSSDFANSTVDFDAYVVASRSETVP
                     STVKTKQGSNTYNNFDTDASVMYQYTADTPEQAKEKVMQYAGRVNGGDLKWTFNNAVD
                     DASYDVNTGLKAALTNYSTSLVAVQGESSSSGSSQTLTVTGDVNQTVTSGNAISNIVF
                     TWGGEATDASVTGLPASGIDYIKDAAAKTITISGTPTATVSYTVTTSGQSGTPASISG
                     TITVTSGSGSSGAMIHNFTTDGKTSSFYSITGNLSTSYGTVTYQGLTLTQCLKMESGT
                     NISFTTTSEATLTLVFNPDYTGTVKIDGTTYTPSSTNGIVTISSLPAGSHTIVKGSGS
                     SYLFYMSTVYNSISGISNTNQSTSIQLYPNPVSNLLNINSNEKVTKIEIFSISGQIIT
                     QLNGNSSSIDLSNLSSGNYLIKIHTQNGVLTKLIVKK"
     gene            complement(24899..>25288)
                     /locus_tag="PLZ54_02630"
     CDS             complement(24899..>25288)
                     /locus_tag="PLZ54_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ65866.1"
                     /translation="DSISSFSAPGVYSDPDGNNIPLETFNYTGGAATLYLYSVSSGVN
                     IYLLDVSSAINYIEPSAVKNPKSENSIAFNGSEIVNAKGLALQVFNASGKRIIASSAT
                     TISTTQLPKGVYIVREVGTSNTLKFVK"
BASE COUNT         7497 a         5142 c         4561 g         8088 t
ORIGIN      
        1 cccttcatgt cctgcttcgt cgcttgcttc aatgtgcaaa tatacaaatt catcggttcg
       61 caaggcattg atggccgctt gagcctttcc ttcatagttg gtattgtaca aacccgtagc
      121 tccttcgaca ttgatcactt tcaaccctgc atacacccca attcccttta ttaaatcgac
      181 tgccgagatg accgatccgc tttttagatt gtaaagttcc attaatgttt tcatttcggg
      241 cttgtaaccg ggcgaccaca gccaaatgct gttggccttg tccttgcctt ctgctgctct
      301 tttcaggtta accggatgat tttccagtaa actctgcgat tgaagagtca attcatttaa
      361 cagacgagcc gttgcttcgg cttccggtac ctcaggttgt atcatcacct ctttgaacgg
      421 tgtgccgggt acatcatgcg gaggcgtgca ttttaagttt ttatttcctc ctttcatttt
      481 taacaaatga cgataagaaa cgccatgata aaaactgatt atttcgttgc caagtttttc
      541 gtttaaaaat ttaattaatt cggcagcttc ttcattactg atgtgtccgg cagagtgatt
      601 tttgattttt ccgttttcta tacaaatcaa attgcaacgc atggccattt cgcccttttc
      661 aatggggaca cccatactgg ctgcttccaa cgaaccacgt ccctcaaaaa cttttgcaac
      721 gtcgtaaccc ataacactta aattggcaat ttcgctgccg ggagaaaatc cttcgggaat
      781 agtatccagc attccacttc ttcccaactc ggcaattttg tctatgttcg gtttattggc
      841 aacttgcaaa ggagttctgt ttcctaaaga ttcaatgggc tcgtcggcca ttccatctcc
      901 caatataatg agatatttca tcagaatata atttattttt taaaggtctg atggaaccca
      961 catgtctgaa tattatattc attcgtgttt gtgtatgcaa tacatgtggg tttcataggg
     1021 tcttttattt gttcaaacta aatttttttt gaaaaacatt ttgatttata acttcaagaa
     1081 gttatggttg tttttcaaat agtgaaaata atttttttat tcttcaaaaa acacctgata
     1141 atcaggcaat gcattcaaca aagtatgaat ggtttcttgc atggaggcat aactttcggc
     1201 tccggcagca ttcagatggc cgcctccgcc aaaaaggtcg gcagctactt tattggcagg
     1261 aaattttcct tgcgaacgca acgaaatttt aattctgttt ttttcttcac gcaaaaacac
     1321 tgaaaaatcg attcctttaa ccgaaagcgg aatatttaca aatccttctg catctccgtt
     1381 gacatagttg aattgtgata actcttcctg cgtgagggaa atgatggccg tacgaaattc
     1441 aggaaaagtt tgcattttct cgtaaaggca aaatcccatc aatcgcaatc ggttttcaga
     1501 ataagtattg aaaacttttc ggtaaatttc atctttatcg attcctatct taatcagttc
     1561 ggaaataatg atataaattt ccggatcgtt ggaattgtag gtaaaacttc cggtatcggt
     1621 catcatgcct gtataaatac attcggcgca ggcaagatta atatttgcaa agtcgcccat
     1681 tcggcaaatc aggcgaaaaa tcatctcgct ggtagaagaa atttccggat aggaaatggt
     1741 aatttttgca aaagattcgg gatgcagatg atggtcaacc aaaattttag gggccggcga
     1801 acttgcaact gcttcggcca tttcttcgag tcgatgcaaa ctgttgaaat cgagcgtgaa
     1861 aattaattct gccgaagcaa gaacgttatc ggctttttcc ttgtcgtttt cataaatcag
     1921 cacttcgttt gctcccggca tccaggaaag gaagttgggg taagcagtgg gtgaaataac
     1981 agttggctct ttttctatcg tcatcagata gtgccaaagt ccaagcgctg ctcccattgc
     2041 atctccgtcg ggattaacat gaacaactat ggcaattttt tccgcatcct gaagcgacga
     2101 gtaaacttta ttgataaggt cttcagctat aatttttgaa atcattgttt cggtttttga
     2161 ttttaaatta caaatttacg tgataatttt caatttacac acagccaaag aaaaatccca
     2221 ttctcgattt tttcaaaata catgaatgct cggaatggga tattttaatt ctgaagaaaa
     2281 aaattgtgtc gcggattaca atttggtgaa aatacaactt ttattgtcgg ctaacgcaga
     2341 gtttttaaag aaatcataaa tttccggata attttctttt ccataaatgc ctttttttat
     2401 tgttaatttt cttatcatca gaaagctgtc tgcctttttt tgacttattt ttgtggaata
     2461 ataaccggca gggcaagaaa aagaaatttc gggatattca aattccaatt tataacccga
     2521 gggaacgtga atagcgatgg tatcggtttg agtaaacgac tgggaaaaat aaacattgtt
     2581 gtttcttttt ttgttcagac caaaatagct tcggctaatt atattgggct tcaaggctat
     2641 tcttttaccc attaattctg catattgaag tatatctcct tcaaaagcaa gttctccttt
     2701 tatgttttta tagctgcttt tattgataaa gctgtagcct gtcagatgaa aaccatttat
     2761 tggtatcgat ttattgatat atttttctgt ttcctgtttc gacagactta aaaatggcat
     2821 tacttcgtcg aaaagttcgt tggaataggt ttttttgatt ttcaaatgtg cgtttcccgt
     2881 ttcttctaca aagatttctc cggaaatgga aagcaaattg tcgaattcat tgtaggaagg
     2941 cgtatcgatt aattttcctc cttcttctgt tacgaccaat gcttgtctgt tgcaatttcc
     3001 cgagccaata tatccgaaag gtatggtatt gctggtacac tccaaccaca aactatcgga
     3061 ttttaatgga acaaagagaa tgacatgatt ggtttggtct acgcttgcaa agtcgggaaa
     3121 ctgtattctt ttattgtttc catttccgat ttcggcatag taagacttga tccccacagc
     3181 attcagtaaa gctctggtat agtttgaaag agccttgcaa tctccatagc catacttgtc
     3241 tacttcggca gccggtaaag gctgaaaacc accaatgccc ttagtaacat tcacatatcg
     3301 aactttattt tgaacatagg cataaacaat tttaactttt tccagttcag tatttgcgtt
     3361 tttacaaaga gaattgattt cttctacact tttggttgga actatttctc tgccggtcaa
     3421 aaaattgaat accattttac cataggattt ccaatcgtta aattctacgg gatattcaaa
     3481 atattttgtt ttactggcat ttacaaatat ttttggcata atcaactgaa atggcaaaga
     3541 aaaaggttct gattcctttg cttttattcc atttattttc caagaataga tgatgttatt
     3601 atttacggtt tcttttgtaa aggtcgggcg aatattttga tacttatatt gaataatgct
     3661 atcaggaaca atgatttgca gttgtgcctt ttcaagtgat tgccggtagt cgtttatcgg
     3721 cacccaactt tcgggcatga ggaggccttt gctttccaat tcgtaaatat attctatggt
     3781 aaccggataa tttttggcgg agaaacccac aacttttatc ctgttgtcgc tgaaaagtgt
     3841 aaacgaagga taagccgcat aatctttaat gttttctttg gataattttt cggtctttat
     3901 cccatttgca ttgtaaatat aaccttcaat ctttttaatg tttgtatagc tgtcgtaggg
     3961 aataatcaaa taagcatttt cttttgcatt atcgttcatc aaggtaatgg caaaagattt
     4021 ttgttttatc aatttatttt cttttatttc gtaactcacg ttactgtaac ggaggatagc
     4081 atcggcattt tctttcaatg aatcgggtat gtttgccaca ttcagatttt cctgagagaa
     4141 gacaaaaaat gtcgaaaaaa aggctaagac taaaaaaaca aatcgcatga ttacaatttc
     4201 tttaaaacaa ttttttcatt ctgttttact accatgtttc tgaaaaattc ttttaacgct
     4261 tcatattctt ccggcaaata aagcaacttg tttctggtat aattcgatac aatcagtatg
     4321 tagtcatctt ttatcgaggt gaagaaaagg aattgtgccg atttgtcagg caaggtaagg
     4381 ttgatagaag atggcaggga ttctactgca tatccatttg gaatttttat ttgacaggtt
     4441 gttttgtctt tgttctggta attgaattcg accggatagg ttctttccga caatttgaac
     4501 ggagattgtt tcatggcttc aaacaacaag ggttttagca tgattaaatc accggcctgt
     4561 tctactgcat tgttgtagga gaaatcaaat ttcatttgaa cgggtatatt cagcgaatcc
     4621 agcccttcta tttgatattc gttcgtttcg atgccgggat tatttttggc gatttcttta
     4681 aaatactcgt cattggatgt gtacgattta atatttttac gaatttccaa tgctgcataa
     4741 ttgtttaata tggaagaata tgttcctttt gcttccaagt tttcatcaag ttcataggta
     4801 agaataatct gacttttgta gcttgcatct ttaagcatat caacccagtt tgcctgccct
     4861 tttttgacta ttaatccttt gtcgttaagg catctgacag gtaattgatt gataatgctc
     4921 aaaggttcgg ttgcatccat caagtagtca ttcccatcga tttttgccaa tgctaccaga
     4981 taattaaaat cggaaagtga tggatgagtc tgtttgatga atccgttgtt ttgtgtggaa
     5041 agtattaccg gataggcctc aaggccgagt ttttgcaaaa gtcctacaag gcaaagattg
     5101 atgtcggcgc aatttcccgt agcattggta tagctatcct taaagccttt tgaggtaaaa
     5161 atgctgtttt tttcgttcca tttataatga ttggcaatgt tataaaaggc aagttccatt
     5221 tttgaatatg gatttttggc tttattgttg atttcttttg ctatatcgtc caaaaaacca
     5281 actttaatgg aattttccaa gtcattatat tttaaatatt gttcaattac attttcccaa
     5341 ctggtggtat agtatttaag cgtggaattg ggaaattttg tgtaagccag ttcaaattct
     5401 acagccgaaa gatagttttt tggagttttc agatagctgt cttctttgaa agccggaatg
     5461 tttcgcccga tatattcgga aatatccaca taaaaattta ttctttgtgt attactgtat
     5521 cttccgttaa aatcctgcca gtgataggtc aagtctaatg attcgttttt tctttcggtt
     5581 tttctatcga tggcatagta gccggcaata ttttgatgaa aattgaaata ttccggaatc
     5641 tcaacttcga aatcagagaa aaggacggga attgaatact gaaaattcca tttgtcgaga
     5701 ttatagaaaa atacggaagt aaccgtataa acaacttcaa taatacttcc tttttttacg
     5761 tttggaagtg gagctttggc tgtttcccaa ttctctgaag tgctttcaaa aatcagattt
     5821 cccttgttga attttgtttc ttcaactttt ccgttattta tattaaaagt agaagctttg
     5881 aaagaagaaa gtttttcttt ttttttgcct gatacataaa gaggtatttt tatgtttgcc
     5941 cagtcgaagg cagattcgtc gagaattttt attctgaagt gtttggtggt ttccaaaaca
     6001 aagccctgat tttcgccggc atcatcttct ctcacgtggg tctgtggata ggtaaaacga
     6061 gaaagcccac aattgaaaat aaaatacgca tgggcatttg agtcaatggg acaaacattt
     6121 tgttccagtt cttctttgcc aacattgcca aattttgaag gagaattttg tgcaaatata
     6181 ccgcagcaag taacgaatgc caacatgaag gcaggtaata aacttttttt catgatatta
     6241 ttgtaaattg atataattca atgtcgtttt tatggaaaaa ttggaaaata attatccccc
     6301 agagaaaaaa tttctctgag cattgatgga atacacagga tgaatactct tccatcaaaa
     6361 aagttaaaat aagttcaaaa ttttaataca gagcaaagtt aaaattatat tttaaatttt
     6421 cctacacatt tttagaaata gcaacaaaaa attttttcct cagagatatt tctaattttt
     6481 ggggacgcat aagggcgaaa ttatttgaaa ggttggtgag ttaatttggc tgaattgccg
     6541 ttttaaagac tttttttgtt gggaatatcc aagatatagg caccttgatc agctacagaa
     6601 taaaactcaa tcccatgttt ctcgcattgt tgtcggatgt cgttggtata ccacttcgag
     6661 atgcttttgt cgacaattat tttttgtggt tgcacacaag aaagaatttg atccattcgg
     6721 ggtttcagat gattgccaat aatcagtaaa tcaatgttta atggagtttc tgtaactttt
     6781 cttttcagtt gttcgtctgt taaaatcaga atctttatgc cgtagaaaga agcaaaacca
     6841 ttttcaaacc agttgctgtt tgaaaggtat tcaggatttt gtaatttgtt tttccgccag
     6901 tagttggatg caattttttc caattctacc gaatctgtag tatagacata attttttccg
     6961 tggtcgataa agttgacatg tgttgatttc tgtccggcaa aaacaattat tttctgattc
     7021 cgtagagtat tgtattttat ttgtaaatcc actacacaaa ccagcagcag cgaaatgagt
     7081 cccaacgcga ggggtacaaa tttcttgttg taatagtatg ccgatagact gaaaattacc
     7141 atgaaaatta gaatggtttg ctttatatcc agagaaatat aagaaagaga gtaggggaga
     7201 tgttcaattt gaacaataca aaaattgaga agcccaacac ttattttcag caaaaaagcc
     7261 acagctgtgg acaaataggg gataaatgaa actgccagca aacccattgc caaatatatt
     7321 acgagtgtgg agagcggtaa aaccaataga ttggtcaaca gaaaataatt ggggaactgt
     7381 tgaaaataat acaatgtgaa aggagcggtt ccaatttgag ctgctactga aacgagaagc
     7441 aaatctcttc cccattttaa aaatcgatga ttggtaggta acaatttagt caggggcggt
     7501 tgaaagaaaa taatgctcag aacggccgag tagctgagct gaaaaccaat atcgaaaaga
     7561 aaattcggat tcaccagcag catgaaaaag gctgacatga aaatcgtgtt gtaaatctgc
     7621 gattttcttt ccagacagtt tgctaaagca acgaatgtga acatgagtgt tgcccgcact
     7681 acagaaggag acaaacccgt aataaaggca tagccccaca tacataaagt gataataatt
     7741 atcttggaaa ttttttgccg ctgattatga ttcagaaatc cgaaaataaa aagcaaaacg
     7801 gcataaactg ttcccacatg aagtccactt accgaaagta tatgtgttgc tcccgaagcg
     7861 ctgaaacttt tccgtaaatc gggttgtaaa tcttcagtat aacccagcgt caaagctgcc
     7921 aaaaccccaa attcgtttcc ttgaatatta aatttcctgt aaattcccag caggtaattc
     7981 ctgcattgat ctgccatgcg acgaatcgaa aaagcttgat tatgaccaat tttttgccag
     8041 tgttgtgaag caatataggc agttgctccc actccctgtc ttttaagata agtagcataa
     8101 tcaaaaccat caggattcag caacttttca gggcttttaa attcagtctc aagcatcaaa
     8161 cggtcgccaa aaaccaaatt gcctgacaaa ctgtcttttt gaagataaat gtaagctttg
     8221 ccatgtcccg attcccaatg tcggttatga agaaattttt ttatctcggc cgtataaaaa
     8281 accgattttg ctttttctac cggagccgaa gtcaattcaa ccacataaat ccctttttgg
     8341 ttcaggggat aaaacttcga tgctttatcg cgttcaaaac taaaaatata acccgtaata
     8401 aaaagaaaaa ggaatacgcc agtgccgaat agccatctga aattgaattg aagccgcgtt
     8461 tcgcggataa aaaaaggaat cagcagtaat gcgattgaaa gaaaaaatag agaccatagc
     8521 gttgcgggaa acaattccgc ataatggtac actacaatgc cgcagatgta ggcaagcagt
     8581 aaccgaatga aaggggttcg ctgtaaaaag ttcatgcaaa atacctgaaa aatgttttat
     8641 ttgaaaccct ccggccggaa aatttttcaa ttttaaaatc gtgccgccaa aggcgagaaa
     8701 gacttttcaa caatttttca tctgctgtat tatttccaaa ggattttcgg ctttgaatat
     8761 cgaacttcct gccaccagca catttgctcc atgagagaaa agcttggggg cattttccag
     8821 attcacacct ccgtcaactt ccagcaagca agtagccttt tggagttcca tcattttttt
     8881 cagctcatcc atgcgttcgt aagtttcttc aataaacggc tgaccgccat aacccgcaaa
     8941 aacactcatc aacaaaacca tatccacttg attgagaaaa ggctcaagca ccttgacagg
     9001 cacatcagga ttgatggtga tgccggcttt catcccttcc tcatgaattt ggtcaattac
     9061 tttttgtgga ttttcggcag cctcaaaatg aaaagtcaac atctcggcgc ctgcttttgc
     9121 aaagcgtgtt acgtattttt cgggatgaac gatcataata tgaacatcca tcatttttcg
     9181 acagtgtttg cgtatagctt ccaatacagg aaaaccaaaa gaaatgttgt tgacaaaaat
     9241 tccgtccatc acgtccaagt gaatccattg ggcttcactt tggttgatca gttcgcagac
     9301 agcctgcaaa ttgctgaagt cggccgccaa aattgaagga gaaatgattt ttctgttcat
     9361 gcgatgtccc ttataaattt agaaaattaa cagctgtgct tttgcaaaaa taacaaaaaa
     9421 tgtaatttaa caagtttagc acatgacaaa aattgtaaca tttgttttat atatctgtaa
     9481 aacaggacat ataagccaaa cacttggggt ttactttgtc cggtgagccg aaaaataagt
     9541 gagaaaagcg tttaaaaatc gtccttgttt gggttccgac gtatttcgtc agaatgagtt
     9601 tgggcgattt cagcttttcg agcgtaagtt tttcgagtga agcgggagca gtcttgaaag
     9661 cagcgcttaa gcgaaaacaa aatcaaattt atttgattta tgttgagcgg aacttcttca
     9721 atgaatttaa tttttttgta aggttctaac tgaatttttt taagttgtca aaaaaacagg
     9781 aagatcttcc tttcctgaaa atcctcctgt ttttattgga ttgaaagtag aaaattatga
     9841 aatcttgatt tcctttacat ttttttcttt cttagctttt tcatgttttg gcaaggttac
     9901 atacaaaata ccgttttcat attttgcaga aatcttgtcc gcattcacaa cttcttcagg
     9961 aacggtaaaa ctacgctgga acgattcata actgaactcg cgacgagtaa ccttttctcc
    10021 ttctttttct tctttttcct gctgtttttc ggaagatatg gtcaacatcc cgtcgtcata
    10081 acttactttg aaatcttctt tgttcaaacc gggagcagca acctctatgc aatattcatc
    10141 ttttgtttct tttacattca ctgcaggcag cgtcatattt tcacctgcaa agttgaatgc
    10201 gtcccaatcc agtaagtcag tatcaaaaaa tctgtcaatc aacgatggaa gtgaaggagt
    10261 tcgattactt ccagatctga aaacttttgg taatgccata attaacacct cctatatttt
    10321 ataagttaaa ccatcaattg ctttttgaaa tcttttttga taaatttcat attttattgt
    10381 tcaaaacgcg taccagcaaa aataaaaatt attgacatca tatctatacc attgaaaaac
    10441 agagctaatt gaccaataga atttatggat ttaaaaaaac aaagcacctt taaagcgtaa
    10501 taattgaata atatctgtcg aaatgtataa aaaactgcca atatgacagg actaaattat
    10561 tcgctgagta tttttagttt gagaaggttt tcaattaata aaaccaatta cactttagaa
    10621 attatccaac aaggaaaaat tgtcaaatat tttttctacc attcaaattg aaatacaacc
    10681 ttctacaaat ttttatatgg actgacgact tttttgaagc tttctttaac atctgagtaa
    10741 aaggttgcgg atagataaaa taaagtacgt aaaagcaatt ttgtaaatga attttctgtg
    10801 atttcaagtt cgccttgtta cgtcgatcaa cgaaatccaa tcattttcct atgaccgatt
    10861 tcctgtaata attttaaaga aaactctctc gagcagaaac gctgaaaatt caaacagacg
    10921 aattaccgaa acctgtcttt gtgagcccaa agtcccaaag caggattgga aatgtagaaa
    10981 atttcttctc cgtcgggcat cacgttccag ccttctttca gagtagaaac gtgatatttc
    11041 gctgtaactt cgtcaatatc accccagcga aaaccaacgc tttctatttc ttctttggtt
    11101 agattttctg gatttttgcc cggacaataa gtaatggtaa atcgtccttc agaagaacca
    11161 tgtattaaat gggcagccgc actcaggttt tgttgcaatt cttcattttc attggttgct
    11221 ttcaatgttt tgggtgttcc aaaataaccg tattttcgaa tcagtcggtc aatttccttg
    11281 tcttcgccaa attccctcaa tgccggtgcc agcacaatca gttcacctcc gtcggcaata
    11341 gccatgcggg ttcggtaaat cgccttattc ccgagccatg tgctcttgaa ttcagccgga
    11401 tcgagccaaa ccagaacttt atttaagggt ttttccacca tttcaaagtt tacttgcatg
    11461 gataaacccg ctgccttgtc aaaaacctcg aaatcgtcgc caatgaaaag cccgtaagtc
    11521 tgcagtttcc cggtttcttt gttcaaagcg accaccgttt gcacatatac aatcggcaaa
    11581 tgtgctgcaa aatgctccga agcatagttg aatactttcc gaacgggcgt attggctcgt
    11641 cccatcattt tttccatgcc gtaaaccgct ccgataaaat gacttttgtt gattccctcc
    11701 tgtccgcctg ttcccacaaa gatgtttttg ttgtaattgg ccatccccac cacttcgtgc
    11761 ggcacaacct gtccgatgga aagaatcaaa tcgaaattac cttccaccag cagcttattc
    11821 acctgggccg gccagtcgaa acttaacgct ccttcggtta cttccgaaac atacgatccg
    11881 ggcacataac ccagcgttat gacatcatgc cgccagtcgt gaacacgaaa aatggttttc
    11941 ggcacttgac cgaacatggt cgcgatttgt tcgtccgtca tgccggtatg cgtgcccaat
    12001 gccggtaaaa cgtccgtgag cttttcgccg taatactgcc atgcaaattc cgtcaactct
    12061 ccggcatgac ttggatagcg agtgaaatcg ggcggaatgg ccagcacctt ttgtcgcttg
    12121 cctaactttt ccaaagcttc gtaaagtccg tttttcaaat cttcaggtgt aaggtcggtc
    12181 gttgccgaac ctttttcaaa ataaatcata atggttgttg ataatgaaaa ttctgggatg
    12241 tagaaacata ctgtaaaaac aattctgaca gagcttttgg aaggcatctg tcagaatgga
    12301 tcaaattcaa aaaacattat tcattaaaca ttgtatgtag tcgaagcagt gtcgccgcct
    12361 cttcctgtcc agttggtgtg gaaaaattca cctctcggac ggtcaattct ttcgtaggta
    12421 tgtgctccga aataatcgcg ttgtgcctgt aaaagatttg ccggtagtcg ttcggtgcgg
    12481 aatccgtcga aataggaaag agcagaagcc atagccggaa ccggaattcc gttttccatt
    12541 gcaacggcac aaactcttcg ccaaccttcc tgagccgatt caacctgttc tttgaaaaat
    12601 ggatcgagca gcaaatgacg tagattcgga ttctggtcaa atgctttttt gatatctccc
    12661 aaaaatacgg aacgaataat acagcctcct ctccacatca gtgcaatgcc gccgtaattc
    12721 agattccagc cgtactcttt ggcagcttgc atcatcaaat cgtatccttg tgcatacgaa
    12781 actattttgg aagcataaat ggcattctcc agatcgttga taaattgctg gcggtcgcct
    12841 ttaaatgctt tcttcggtcc tgaaatggct tttgaagctt ccacgcgtaa atctttctgt
    12901 gccgacaagc aacgagaaaa aaccgattca ccaatcagag tcaacggaat gcccaaatcg
    12961 agcgctgcat ccactgtcca tttcccggtt cctttttggc cggccgtgtc gaggattttt
    13021 tccaccagcg gcgttccgtc ttcatcttta aatgcgagaa tgtctctcgt tatttcaatc
    13081 agataggaat ccaacttgcc ggtgttccat tttttgaaaa cttcatgttg ctcgtcatcg
    13141 atcatgccaa gcaattcttt cattaaaaag taagcttcgt tgattacttg catgtcgcca
    13201 tattcaatgc cgttgtgaac catttttaca aaatgtccgg caccattttc gccaacccaa
    13261 tcgcagcatg gcgttccatc ttccactttt gcggccacag cctgaaaaat atctttcaca
    13321 taaggccaag ctgcaggaga acctccgggc atcatggatg gaccttttaa tgctccttct
    13381 tcgcctccgg aaacgccggt accaatataa agcaagcctt tgctttcgac atacttagta
    13441 cgacgtatgg tatcagggaa atgactgttt ccgccatcaa tgataatatc gcctttatcg
    13501 agcagcggaa taagctgttc aatcaaatca tctaccggtt gtccggcttt caccagcatc
    13561 attactttac gcggcctttc cagcgaatcg accaattctt ttaacgaatg agcgccgata
    13621 aaatttttcc ctgctccacg tccgttgata aatttatcta ccttttcgac cgtacgatta
    13681 taaacggcta cagtgaagcc tttactttcc atgttgagta ccaaattctc acccatgacg
    13741 gccaatccta tcaagccaat atctgccaat tttttcatat tttttctgtt tttatttcaa
    13801 tattctgttt tataaaaaga ggtttcttct gtcgaatttc tatagctttt atctttttac
    13861 gcgggcattt ccttcccaaa tactccaaat ttgttcttca tcggccggtt gagcatagtc
    13921 agtaaggaaa gtggttgcca atgcaccgct tgcccacgca aattgggtac atttttcgat
    13981 attccactct ttcagaatcc cgtaaagtaa accgcctacg aatccatcac caccgccaat
    14041 ccggtcgagt acgtaaatgg gtcgtggttt gatgacctgc caatcttttc cattataaac
    14101 aatagcaccc cacaaatgtt catttgcact gatgacttca cgaagcgtat ttccaaaaac
    14161 gctgacattc ggaaatgtta cctgtgcatt cataatcatt tccttaaaag aatcaatggc
    14221 attttccaac cctttgcctc cttcggccgg tccttttatg cccagacaaa gttggaaatc
    14281 ttcttcattc ccgattaaaa tatccgacaa agaagcaatt tcggagaaag ttgtccttaa
    14341 ttcggcttcg cgtcctttcc aaaaagtagc ccgataattc aaatcaaaag aaatgagcgt
    14401 tccttctttt tttgcatagc gtgcaatttc aagacaaaaa ttgctcgttt ccggcgaaag
    14461 cgcagcaatc aatcccgaca aatgaattat tttcactcct tctttggaaa aaattcgttc
    14521 cagatcaaaa tccttcacgt tcaacgttcg tcccacttca ccggcacgat cgttgagcac
    14581 acgcggccct cgcaatccat aaccgctgtc ggcaatgttg aactgatgac gatagcccca
    14641 aggaccacct tgagggattt ccggtccttc aaaatcgatg ttccgacgat gcaagtcgga
    14701 cttgataaac gaagcaatcg gactgtcctt tacgaaagta gtcaacactt tgaccggaag
    14761 acctaaaaat gaggaaatac ttgccacatt ggtttcggca ctggtagcct gcatttcgaa
    14821 caattgactg ctatgaacag gttgtccatt cacaggagta atacgtacac ccatgctcgt
    14881 aggtgtcagc agggcatatt ttgcattttt ccgtaattga agcgacatat caatattatt
    14941 tttttattaa attaatgtag ttcgatataa gaatatttta taaaccatta taaataagct
    15001 aataaggttg atggaaagaa aatattcgca tgttactaat gttagttatg tctaaataag
    15061 gtctttatat tcaaaaaaga accttatttt tttaataaaa accttagaaa agatgatcaa
    15121 tatcaagaat aaaccttatt acctgtcaac ttttttatat aattaaaacg tgtaatcgtt
    15181 atttcgaaat catgtttaat ttcatcagaa tataatttat tttttaaagg tctgatggaa
    15241 cccacatgtc tgaatattat attcattcgt gtttgtgtat gcaatacatg tgggtttcaa
    15301 ataaatgttc tgtaagactt ttcggtactg aaaattaaat gcaaaaggca tcaaatccgc
    15361 cgtcaacaat ggcaacagtg cccgtaacaa atttagaagc atcgcttact aaataatgca
    15421 tggttcctaa caaatcttcc ggaacgccaa accgacggaa aggtgtatga gcaataacgg
    15481 cttttccacg ttcggtataa gaaccatcag gattaatcaa taaagtgcgg ttttgctccg
    15541 taataaaaaa tccgggacaa atagcattga cgcggaagtt ttcaccaaac ttggtagcca
    15601 gttcgcctgc catgtatttt gtaaagttgg ttacggctgc tttggctgct ccgtatccgg
    15661 ctacgcgagt caatggacga agtgcagaag ctgacgaaaa gttgatgata tttcctttgt
    15721 tggctttgac catgcattcg gcaaaaacaa ttgtcggaat gacagaaccc atcaaattca
    15781 aatctaccac tttacgaaag tcatcgagtt tcaggtcgaa aatggtgttg ttcggtccaa
    15841 tggtagcacc gggcatgttg ccaccggcag cattgatcaa cacatccact tttccgtact
    15901 tcgcaaggat atcttcgaca ttttgtctta aaatggtttc attggtaaca tcagttacca
    15961 gaaacatagc ttcgccaccc tttcccttca cttcttcgac gagtttgtcg cctttttcct
    16021 tgcttcgagc cagaacagcg atttttgctc cgtgtgcagc caaatatttc accatcgatt
    16081 cacccagaac gcctgttcct ccggtaaatg cgataacttt atccttaatg tcaaaaagtt
    16141 cgtacatgat atttattttt agaattatgt ttcaaaatta taaaattaat tcattctttc
    16201 tgagagagtt ttcacaaatc gaacaatctt ttcaaatgtc gatcgtaaat attttcctct
    16261 acacactggc caataatctc ttcatatttt tcgagcaatg aaatatattc atctcccatt
    16321 tcggcagcaa ctttgtaagc tacatgaatc aactgtcgga aattgggatt ataatcgggg
    16381 caagtttggt catgacgcaa cgtgtttacg aatttttcac ttccccatgc gttaacttct
    16441 tccactgacg gcagtttact tccatcaata tcaatcacat cagcataagg tgcgcataat
    16501 tcatcttttc ggttataaga gttggcgtag atttttttag ccgccatcag tccatctcct
    16561 ccggctttag ccaatccaat aacttcctcc aaccatgttg ttccggctgt tttaaggtgt
    16621 aagcctttgt cgtgtttttt aatgattcgg ctcataacgg gataaatgga aaacttgtcg
    16681 ctgcccgaat gaacgcttaa tttcaactct tccggaagtc caaattcttt cacagcaaaa
    16741 tcgataacca acacatcttc ttcaaattct ttagcaaatt gttccaaatc gcctgcataa
    16801 tcgactcctt tgttaaatcg gccggtaaat ttgggcgcta tggtttgaac cggtacacct
    16861 ttgtcggcca acattttaag tataaaaaac aattcaacag gcgtttgagg agactccact
    16921 tcatccatcg aaacttccgt aataaaatta tcggctccct tttctttttt gagataggca
    16981 taaatttcag aagcttgttg cgtagcagcc aaaaatttat ttgttatttt ttccagcaga
    17041 tcctttgaca cattcaatgg ttcctttaat ccgggaattt caagttgccc catgtacttt
    17101 ttacaggaat caataaattg attcacctct tccttagtac tttcttttcc gatatacgca
    17161 gccacatcga gtgtaaagaa atcggaaacc tgtacaaaag gagcaaccgt attcaaatta
    17221 atatggtcgg catcaacaaa atatggccct ttataattta aagctttaac agcattgtcg
    17281 gcttccactc tcgtatcttt cggttgagaa tggacataag tatgctcacg attcgattta
    17341 ttccatacgg gaataatatt taatcctttt tcattggctt tcattaaagc tttcaattgc
    17401 gcttttccct ggtgcgaaaa tctgtctccg acaccaaaac tatattttcc taatttcatt
    17461 atgaatattt tttaaatgaa taaaaatcaa aattcaaaaa agttttttgc attgaaataa
    17521 gaaatgtttt ccaccatttt tcccagaaag tccatttcgg aagcaggcag caaaccgttt
    17581 tccacatcat tgcctatcaa attgcaaaga atacgacgaa aatactcgtg tcgcggataa
    17641 gacaaaaagc ttcgtgaatc ggtcaacatt ccgacaaaac gactgagcaa tcccaaattg
    17701 gatagtgagt tcaattgagc ttcgatgccg gatttctgat cgagaaacca ccatgccgca
    17761 ccgaattgca tttttcccgg aacgctacca tcctggaaat ttcctatcat ggtggcaaca
    17821 agatcgttgt cgcgtggatt tagattgtaa atgatagttt ttgtaagctt tccggaaaca
    17881 tccaaactgt cgaaaaatgc actcatggct tttgcaaccg taaaatcgcc aatggaatcg
    17941 aatccggtat cgggccccaa ctgcttcatc atcctggaat tgttgttgcg aatagctccg
    18001 taatgaaatt gctgtgtcca acctttttcc caatccataa cagcgccttc gtaaagcatg
    18061 gccgatttga atttacgaat ttcttcctga gttaattcct taccaccgta aactttgttg
    18121 aaaatgactt caatttccga ttttgaccac ttttctgcat aaaattcttc aattccgtga
    18181 tcggaaagtt tgcagcccaa atcggcaaaa aaatcatgac gaacgcgcaa tgcttgtaac
    18241 aaatcatcaa atttgcgaat ggtaacgcct gaaacttccg aaagtttctc gacatatttt
    18301 cgatattcca ccggattttc caccgccatt gctttatcgg gacgccaagc cggcaacact
    18361 ttgataccga agccttcttg cttcaaagct atgtgataat tcaggctgtc aaccggatca
    18421 tcggtcgtac atacaacttc cacattgaat tttttcatta agccgcgtgc cgaatattcc
    18481 ttcgtacgga gcttagcggt acattcatca aaaatttctt ttgccgtagc cgggtttagc
    18541 aatttatcca cgccaaaagc ccgtttcaat tccaaatgcg tccagtgata cagtggattg
    18601 cgcatggtat aaggaacagt ttcagcccat ttttcaaatt tttcccaatc tgaagtgtct
    18661 ttcccggtac aatatttttc gtcaattccg ttggagcgca tagcacgcca tttgtaatga
    18721 tcaccttcga gccagatttg tcccagattg tcgaactgac gatcgttggc aatgtattcg
    18781 ggattcaaat ggcaatgata atcaataatc ggttgatgtt cggcatgttg atgataaagc
    18841 tcttttgctg catcactttg cagtaaaaaa tcatcgttaa taaaactttg cataatattc
    18901 tgttttaaaa gattattttc tgtaaaaata taaactgtgt gcgcacacaa agttataaaa
    18961 atattctcat tttaataagc atataaattt attttcataa tattaacaaa tgaaacgatc
    19021 aaagatgaat gaataacctt taaaatggat tcaatttcat tttttattta tactgatcga
    19081 cactttctga ttcagacagt taagctcatc atctatggca tttataaacg atgctttatc
    19141 ctgaattggc tgccattcct tttcccacgt agccataaaa taatattcaa cataaccatg
    19201 atccggcgta agaacgataa cgtgattcag ttcgtcttcg gtcaaagtct gcaattgcct
    19261 ttttctataa aaaatcgcaa gtcccatatt atcattattc atgctctgac aaccaaaagt
    19321 agcataatat gtccattccc cgtcgttggc tggaggcaca attatttcgg tattcttcaa
    19381 cttaataatt cctgtggcta tattatccaa tttcccatca attttcaatt ccatgtgcga
    19441 agcccgatgc cctgcatcaa tggttatcag tgaagttaaa ttgactttgt ttgctgcttc
    19501 ccaaccgtaa taaacggttt ttatttgtga acgaactttc ccatccgaaa tgacacaaca
    19561 caccaaactg tcgcgttttt cgactctgaa agcttttttc ccatcccaat aagcaatgct
    19621 gccaataccc agcgacttgc caacttccat atcgtccatt ccccaaacgg ccattttatg
    19681 ataattgtcg taaccatcaa cgcccactgc cggtaaaaca attccgggcg tttttttccc
    19741 aaaggcatcc aacgcatttc gctgatcgag gtaaaatcga taggccacct tgtcgctttc
    19801 ccagccggct ccttcatatt ttataaaata ggaatgatcc ttaaaatttc cgggcaaacg
    19861 gataaaattg ggcttcaccc acgaaaattc accctgatat tttccgttta caaactgacc
    19921 tccaattttg tgggcaattt ctgcataggt tcgtttggga taattttcag cttgtccacg
    19981 ataaaagtta aattttaata tagcattcgg ctgcaatctt tcaatcgaaa aaactgcgat
    20041 ttgttcagca tttatatcgg aaacaatttc aaccggcaat tgaacgcctt ctttcgaaac
    20101 tacataataa tttccaatgg ttaagggcaa tttattcacg agaatttcga ccgtaaaatc
    20161 cttcaacaac acgttggatt gatttttaac ggtaaccgac tgtgaataaa cattatgaat
    20221 aagcaatgca aaaactatta aagatattga tctactgatt ttgaacattt tactttatat
    20281 tttaatgatt ttctcgattt ttctcaaaaa aatcttttcc gagtactatt tccttgtcga
    20341 aatttttaac ttgtcgggat gaaagcgttt ttgaccattt cactcgacga ttggtcaaat
    20401 taccaccttc acctgaattt tgatattccg aaaattgagc cgttttttca ttggaaactt
    20461 ttccccaatt gtcccaccct tcgggtttta tgacgttggt catgtcgcat tcgataaaag
    20521 ctacttttgc ataaggacgc cacggtcttc ccaaataaaa tgaagccgga ttttcgccat
    20581 gaatttgaca acgataaaac acaaatccat agttgttctc ttgaggcgtt gctgctgcgg
    20641 ttgtaaatcc gctttctttg cagtaaagtt cacaagagtc aaaataggca gttgaaaagc
    20701 caaaaatgaa atcaaccgtc ccttcgatgt aacaattttt atagtattgc ctgctgccgg
    20761 ctttgtgtgg ataaagtgtg tcctgaaatc cccaaaaatt acaatttaaa aaacgtgcct
    20821 tatcgcttcg aacaattgcg gcaaccgcct gaccaattgg gcctgcagca ttctcgaagg
    20881 ttagattttc agccgtaaaa ttgggtgaac aaatataaac acttgcagaa ccggaagttc
    20941 ccagttgatc accaaaacca ctcggttttg aagcaaaatt attgtacgaa agaatcgttt
    21001 tattcctgtc ttctccaatc aaggtcaacg aatccttcgt ttcagggatg atgagttttt
    21061 ctctgtaaaa tccgttgcga ataaaaatac tggttttcct tttgcgaaaa tcgggaacgg
    21121 cattaatggc ttcctgaact gttcgaaaat cgcccgttcc atcctgagca acgatcagat
    21181 catatttcga atgatcgact gcaattggag tattgtataa aattcgattt agaaaattgg
    21241 caacatattg aaccgtagat aattgccacg gatgaaaatg ccaaaaagaa tgaggacaat
    21301 catcaaaagc atgtacttcg gaataaatgc ccaaactgtc caacattcgg atatgttcgt
    21361 ctctgccatt atgaaaacgt ggaatggaac tgttgatgaa acagactgga gcagaattct
    21421 gattgaccca ggttaccgct gaagcagctt tccaattttc gggacgttcg gcaaatgttc
    21481 cgcccagcca caaagcatca accggcattt tatctccaat gtttcgagct ttttgcgctc
    21541 tgtcaatggt cgctttgtcg ataaaggaag aaatgccatc gatattgaca acggttttga
    21601 tgagcttatc tttatttttt gttccgatta aagcagccaa ttgtccccct gccgagcatc
    21661 ccgaaacggc aattttatcc cgattgattc cgtaagtttc tgcgttatcc gcaatccatc
    21721 ttacggcatc ttccaaatct tcttctccgg caggaaataa agcttccgga ataagacgat
    21781 attcgacggt aatacagaca aaaccttttt gagccagttt cactgccatt gctttttgta
    21841 atgaaggaga acctgaattc caacctcctc catgaatcat cattatagca ggtaaaactt
    21901 cattgttatc caaccgataa atattcaaca acagcttcct ttccggtgaa atgaatttgt
    21961 atgcaatgtt ttcgtacgat ttcagattgt cggacattct tgcctccgcc gggcgaataa
    22021 aaggatactg ttttttcaac ttgatataag tactgtagac cgtgtagctt gtatctcgcg
    22081 gaacaccgtt tatcattact gtttgtgcct ttacgggaaa gcataaaaca atcgcaccaa
    22141 aaagaaatat tgaatgagaa aatttcatga taaaaagtta aaatacaata caaagggtga
    22201 aatagcagtg aatgaatatg gtcttgaata aacttcactg ctatttaccc aaaataagaa
    22261 ttatttcttc acgatcagct tggttaggac tccattttgc gtatgaattt ttattaaata
    22321 attgccactt gacaaattgc tcaaatcaat actcgagctg tttccattta attgcgtaat
    22381 tatctgacct gaaatactaa agatttcgat tttcgttact ttttcatttg aattgatgtt
    22441 caataaattg gaaacaggat ttggataaag ctggatagaa gttgactgat ttgtgttact
    22501 aattcctgaa attgaattgt aaacagtact catatagaac agataactgg agccgcttcc
    22561 ttttacaatc gtatgagatc cggcaggaag tgagctaatg gttacaattc catttgtaga
    22621 ggacggcgtg taagtagttc cgtcaatttt aacggtaccg gtataatcag gattgaatac
    22681 cagcgttaaa gtagcttccg aagtagttgt aaagctgata ttggtacccg attccatttt
    22741 aaggcactgc gttaaagtta aaccttgata agtaaccgtt ccgtaacttg tggatagatt
    22801 gcctgtaata gaatagaaag aactggtttt accatccgtt gtaaagttat gaatcattgc
    22861 tcccgaagaa cccgatccgc ttgtaacggt aattgtgcct gatattgaag ccggagttcc
    22921 tgattgtccg cttgtggtta ccgtatagga aacagtagct gttggtgtac cggaaatggt
    22981 aatcgttttg gcagcagcat cttttatgta gtcgattcct gaagccggca atccagtaac
    23041 tgaagcatct gtagcctcac caccccaagt gaaaacaata ttactaatgg catttcccga
    23101 tgttaccgtt tgattaacat cacctgttac agtcaatgtt tgcgagctgc cggaagaact
    23161 actttcaccc tgaacggcta ctaaacttgt actataattt gtaagagccg ctttcaaacc
    23221 cgtatttaca tcataagatg catcatcaac agcattgtta aatgtccatt tcaaatcgcc
    23281 accattaact cgtccggcgt attgcattac tttttctttt gcttgttctg gggtatctgc
    23341 tgtatattgg tacatgacag acgcatccgt atcgaaatta ttataggtat ttgagccttg
    23401 ttttgtcttc actgtcgaag gaacagtttc acttcttgaa gcaactacat aagcatcgaa
    23461 atcgacagtt gaattggcaa aatcagagct tccataagca acaaaacgat tttgaccggt
    23521 catgtaattg ttaaatgctt tgataatacc gccatcttcc cctgaaaaag taccacttga
    23581 ctgatagata tcagtacctt gcttggaaat cagcatggga tatttgcaat tgcggaaata
    23641 atttccttct acaaatacag aggcacctgt ggttgagcca acaccgtatt ttgcatttcc
    23701 atcgtaataa ttattataaa catgggtaga ataatagcga acgcgaggat ggcgagaatc
    23761 tgaatgatcg taccaattgt gatgataagt tgcataaagt cctgaagtgg ttccttctcc
    23821 cagacctaac aaattgcatt ttccacaatc ccaaaaatgg ttgtaagaaa aagtaacata
    23881 agtagatttt ttacagtcaa gtgctccgtc tcctttaatt tgatcggcat cgctgccggc
    23941 ttcaccatag aaaaagtcgc aattatgcac ccagatataa tcattatttt gttgtaagct
    24001 gacattatct ccttcatcgc tgtcgcaatt cataaaacca agatttctga cttcgatgtt
    24061 agaagcattt tttatacgta atccccaacc atcggctact gcatcgtcgc caattccttc
    24121 aaaagtgata taacttgaag cattattgtt gttctcgatc acaatatctc cattcaacat
    24181 ataatcaagg tcggtaattt gtccaatcaa tcgaaccgat aaaggacgtg tatctttccc
    24241 ttttttaaaa ccatccaata tgctttgcag tccaacgcat ggattcgttg ttgcaccggt
    24301 aacagtcatg gataccgaat ttttattact ttcggtaata taaagaatca ccgcattgtc
    24361 tttcaatgtt ccatcacttt tataagctcc cggaacgcga ctgccctgaa atgcaaaacc
    24421 gcttctgtcg tgagctttca ccattatggc acttaccgaa gctccttcag cttccgttcc
    24481 atttactaca ggtttcaccg taaaactgta ttctccttct gccaatccga caatatcagc
    24541 tctccaataa tctgcatatt tacggattaa tggactgtca atttttttgt ttgtaatgcc
    24601 accgccggta taataaacat tgtaactatc aaccgaaccg gaagaatgcc atttaagaca
    24661 agcagactcc agccatccgt tggcttcaaa aatatctatt gattgagaat atgccgataa
    24721 agaaaagaaa aacattaaat gaagaaagag aataacagtt ttgttcatga tgtaagattc
    24781 taaattttaa agttggtaac taaattaatt ggaatagtta gatttgtttg aataaataat
    24841 ggataacaag ctgtccaaaa tctttgaaca gcttgttaaa actacaactt gtttaaaatt
    24901 attttacaaa tttcaaagtg ttggatgtcc ccacctcacg aactatatac actccttttg
    24961 gaagttgagt agttgaaatt gtggtggcgg aagaagcaat aattcttttt ccacttgcat
    25021 taaaaacctg cagtgctaaa cctttagcat taacaatttc tgaaccgtta aaagcaatag
    25081 aattttcgga tttaggattt tttactgctg aaggttcaat atagttaatt gctgaagaaa
    25141 catccaataa ataaatgttt actccactac taactgaata taagtacaga gtagcagcac
    25201 caccggtata gttgaaagtt tctaaaggaa tattatttcc atcaggatca ctataaactc
    25261 ctggagcact gaaagaagaa attgaatc
//
LOCUS       DAOSCH010000017        23653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1550_length_23653_cov_6.020171, whole genome shotgun sequence.
ACCESSION   DAOSCH010000017 DAOSCH010000000
VERSION     DAOSCH010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1550_length_23653_cov_6.020171"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..615)
                     /gene="efp"
                     /locus_tag="PLZ54_02635"
     CDS             complement(46..615)
                     /gene="efp"
                     /locus_tag="PLZ54_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOJ65867.1"
                     /translation="MATTADIKNGMCIELDGQYYIITEFLHVKPGKGAAFVRTKLKNV
                     VTGRTIDKTFNAGVKIDEVRIERRPYQYLYQDDMGFNFMNNETFEQVSVNKELINGVD
                     FLKEGMICDVVTHAESETILYVDLPTHVTLQVTYTEPGLKGDTATNATKPATVETGAV
                     IRVPLFINEGELIKVDTRDGSYVERVKTV"
     gene            complement(814..4344)
                     /gene="nifJ"
                     /locus_tag="PLZ54_02640"
     CDS             complement(814..4344)
                     /gene="nifJ"
                     /locus_tag="PLZ54_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOJ65868.1"
                     /translation="MAKQKKFITCDGNQAAAHIAYMFSEVAAIYPITPSSTMAEYVDE
                     WAANGRKNIFNEKLQVQEMQSEAGAAGAMHGALQAGALATTFTASQGLLLMIPNMYKI
                     SGELLPGVFHVSARSIAAQALSIFGDHQDVMAVRQTGCAMFATGSVQEIMDMAAVAHL
                     SAIKSRVPFVHFFDGFRTSHEIQKIEQLDQEDLIPLIDQEALKAFRNRALNPEKPVIR
                     GTAQNPDIYFQAREAANPFYDAVPEIVEDYLNKLGEITGRKHGLFDYYGAPDADRVVI
                     AMGSATEAIKEGVDYLNAKGEKVGLISVHLYRPFSAKHFLSVLPETAKRICVLDRTKE
                     PGASGEPLYLDVKDVLYAKSNQPTVVGGRYGLSSKDFTPAQVLAVFENLALPEPKNHF
                     TVGIVDDVTFTSLPLREEIPMGGKGIFEAKFYGIGSDGTVGANKNSIKIIGDNTDKYC
                     QAYFSYDSKKSGGFTCSHLRFGDEPIRSTYLVTTPDFVACHVQAYLRLYDVTKGLKKN
                     GTFLLNTIWDEEEVKKNLPVKVKKYLAAHNINFYIINATKIAQEIGLGNRTNTILQSA
                     FFKISNVIPYELAVEKMKYAINKTYGKKGEDIVNMNYAAVDRGGEYVKVEVPSNWANL
                     EDEKEMEENFPPFIREVVMPINAQAGDDLPVSVFKGREDGTWDSGTAKYEKRGVASFV
                     PVWNSANCIQCNECAFVCPHAAIRPFVLDEAEQAKAPFTDTIKANGKQFVGMTYRIQV
                     DVLDCMGCKNCADICPGTKGNKALTMVPIETQYDNQKNWDFCISEVKSKQHLVDVKMN
                     VKNSQLATPLFEFSGACAGCGETPYVKLITQLFGDRELVANATGCSSIYSASAPSTPY
                     TTNENGQGPAWANSLFEDNAEFGYGMELATEVMRDRLERIMKEAIESNCCSDELKALF
                     AEWIENKEDAEKTKELAAKITPLLKNCDCEHCRKISELSHYLVKKSQWIIGGDGWAYD
                     IGYGGLDHVIASGKNVNILVLDTEVYSNTGGQSSKSTPIGAIAKFASAGKRIRKKDLG
                     MMAITYGYVYVAQIAMGASKAQTLKAIREAEAYNGPSLIIAYSPCISHGIHNGMEKAQ
                     EEQERAVECGYWQLYRYNPDLEAEGKNPFILDSKEPQWEKFQDFLKGEIRYTSLMKQF
                     PAEAQELFIAAENNAKWRYKMYKQMADRQF"
     gene            complement(4390..5571)
                     /locus_tag="PLZ54_02645"
     CDS             complement(4390..5571)
                     /locus_tag="PLZ54_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004307073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ65869.1"
                     /translation="MESFFRTHQYLVEHVQSPVRRTLMDEIDWTDRLIGIKGSRGVGK
                     TTFLLQYAKDFFRSNDQSCLYINFNNLYFTTHTLVEFAERFVKQGGRTLLIDQTFKYE
                     NWSKELRECYERFPELKIIFSGSSVMRLKEDNEDLQDVVKVYNLRGFSFREFLNLQAG
                     TNFQPIDLEDIIKNHVPIANEICSKIDPLLYFQDYLHHGYYPFFLEQRNFSENLLKTM
                     NMMIEVDILLIKQIELKYLSKIRKLLYLLMISAPATPNISQLSCEIQTSRATIMNYIK
                     YLKDARLLNLLYNDNEQFPKKPHLIYVHNTNLMHAAMPGNIDRLSEIKTFLYNSLHAK
                     YKVNSGKYHSDFTVDKQYTFKCELNSNISKQHSKLIFATDQLKIGKKNEIPIWLFGFL
                     Y"
     gene            5884..7287
                     /locus_tag="PLZ54_02650"
     CDS             5884..7287
                     /locus_tag="PLZ54_02650"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ65870.1"
                     /translation="MKIKFIGAAREVTGSKHLITTKDGKRILLDCGMFQGKGLETDAK
                     NKDLGFDPETIDHIILSHAHIDHSGLIPYLYRNGFRGSVICTNATRDLCSIMLADSAH
                     IQELDIKWFNKKRARQGLPPVEPIYDKEDAENCMDLFIGVSLNRRFYIDNKINVKFTN
                     TGHLLGSAVVNLEIIENGKKVHIAYTGDIGRSTDYLLRSPDPFPQCDYLIAESTYGNR
                     LHPETNQTEEDFLKIITETCIKNKGKLIIPAFSIGRTQELVFLLNNFYNRHLLPHIEV
                     FVDSPLSVNATEIYRMYSELMNKNVKEVLKYDNDPFGFNSLHYIKNVEESKALNTYDK
                     PCIIISASGMMEAGRIKHHVANNIENPRNTILIVGYCSPTSLGARIQQPGLREISIFG
                     EIHPVNAKICKIEAFSGHGDYKEMIDYLGCQSAGLIKKTFLVHGDYEVQVEYREKLQK
                     EGFRKVEIPDAKEEFIL"
     gene            7637..7891
                     /locus_tag="PLZ54_02655"
     CDS             7637..7891
                     /locus_tag="PLZ54_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOJ65871.1"
                     /translation="MTKMITFNELRRIKDALPHGSLSQIAEELGLDIETVRNYFGGHN
                     YKDGKSCGIHIEPGPDGGLVMLDNTDILEAALRILGKEEA"
     gene            8131..9477
                     /locus_tag="PLZ54_02660"
     CDS             8131..9477
                     /locus_tag="PLZ54_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA cytosine-C5-methyltransferase"
                     /protein_id="HOJ65872.1"
                     /translation="MNLSPAFEKRMQVLLEEEYALFRAALEKPAPVSIRANNKIVYQP
                     SKEQVSWCESGFYLSERPVFTVDPFFHAGVYYVQEASSMFLRQCLKQYFSQAETVLDL
                     CAAPGGKTTLFSQYINENALLVSNEINRSRAYILAENVAKWGNANVVVTNNQPADFKN
                     LPGFFDAMLIDAPCSGEGMFRKNPASIDEWSLTNVENCVVRQRKIIFEVWDTLKTDGI
                     LVYSTCTYNRQENDENIKWICEELGAEILRMDIQHFQGILETEAGYRFFPHKIKGEGF
                     FISVLRKTSPTPQKMRKAKKIERKSDDKIKSEVGIKLLEPEKWNLFMENNRLRAVSKA
                     RQKEIAIIENNLKCLLSGIYLGDYKNSDFIPSAHSALSKETDLKAVETVEIDYTTAVR
                     YLKKENIQVESDKKGFLLLLYKGVPLGWVKNIGQRCNNLYPQEWRIRMKIEELEEI"
     gene            9474..10220
                     /locus_tag="PLZ54_02665"
     CDS             9474..10220
                     /locus_tag="PLZ54_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004321259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ65873.1"
                     /translation="MKILLLGTGTSTGVPQIGCNCYTCTSTNIKDQRLRSSLLVSEKN
                     TRILIDCGPDLRQQLLNYRIHDLTGILLTHEHYDHIGGLDDLRPLGPSHLFGEKRVLK
                     VIERNMPYCFGDKRYPGVPELFLHEINEEEFSLDGLKVQPVRVMHANLPIFGFRIGKM
                     AYLTDVKTLDEKAVEQLQQLDVLIINALRPFKHISHLSLSEAIELAEKINAKETFFTH
                     ISHDMGRHNEINSVLPPHIHLAYDGLELAI"
     gene            10317..11342
                     /locus_tag="PLZ54_02670"
     CDS             10317..11342
                     /locus_tag="PLZ54_02670"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOJ65874.1"
                     /translation="MKKSFIIFVGLSIGAMLFSSCSNMLYTSLDVLRPAKVAFLPNVN
                     RLLIVNNTPVMALENDPNKTKKNVTLPADSLPIYCLSVLAQELGNNSFFSSVLLEPNS
                     IYPKNNYSEKLSTEMVKKLCENYNCDVILTLDSLKASDVLTEYYLEDISQYLSTYEIY
                     LKSKWSIYYPSQSKPVSLQFKDTVYWEAQDYSRQRALKQMPDRWDALVDGALYVGKNS
                     VKRFIPYWDKVDRYFFNPGNKLMRQAMDSVYVRNWTSAIELWKKAMNKSKSEKLKGEA
                     ANNIAIAYEILGDMDNAIAYASQSLQYFSSVSIIDYYTLERINNYYYELIKRKNEIQQ
                     LKLQLGE"
     gene            11416..12942
                     /locus_tag="PLZ54_02675"
     CDS             11416..12942
                     /locus_tag="PLZ54_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOJ65875.1"
                     /translation="MELLLPSDEILLQEKGIGKQQIEEQLHYFETGFPFLKIVAPAVI
                     GNGITIIDDKKGEFYINKWESYRQKEGIDIVKFVPASGAASRMFKDLYEFLESEAELP
                     QKSSVKDFFNQIEKFAFYEELNKACLKNEGKSVAELIKNNRQKTVVENLLLEKGLNYG
                     FLPKGLLLFHSYPDEKRTPVQEHLVEGALYATHADNDVKIHFTVSHQHRALFEKHIQE
                     ILEKYEQKFKVHYHIDFSEQKPSTDTLAVDMNNRPFRENGQLVFRPGGHGALLENLND
                     LLADIIFIKNIDNVVPDSFKEITVKYKKIIAGVLIDIQQLCFDYLKELENNNLTENRL
                     KEILRFTEETLCIRHPEIDSFDTEALKKYLFRKLNRPVRVCGMVKNTGEPGGGPFLTV
                     NADGTISPQILESSQIDLNNPEEKQKLENSTHFNPVDLVCSVKNYKGEKFDLTKYVDK
                     NTGFISIKSKNGRELKALELPGLWNGSMSDWNTVFVEVPIETFNPVKTVNDLLRPQHQ
                     "
     gene            complement(13004..13085)
                     /locus_tag="PLZ54_02680"
     tRNA            complement(13004..13085)
                     /locus_tag="PLZ54_02680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13049..13051),aa:Leu,seq:tag)
     gene            13417..15855
                     /locus_tag="PLZ54_02685"
     CDS             13417..15855
                     /locus_tag="PLZ54_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ65876.1"
                     /translation="MKTKLLLSLGLLGTMSFLYGQQYRPGKQIDHDNTAFVRKVELKK
                     AGEVIMKVDQISVTDTTSELSASIYLPDFTLFKNFHAPVPADGAQFFFGHGGVTVAEN
                     LVNNQPRYYFLYQTEGYSEDESGFRFFQDIHIIDENQQQVGKVPTSWMGYQLYMQDGK
                     NMLISDYQDWDTIFWSNPETGWSGWETFQVHTSVVTDVATGQTIFTFPRGTTFSNFNG
                     MQSGIYYATNEQNLFFNIDGEERILTTNNNFEKYFKGHSSGNHENALDMWIYNKDFSL
                     YKKISNVGYDIPIENLYMANTYQPLFGKHAAYFYHTVTYQTDGGLNYNSKTYLVNLNG
                     ELVKIIDGNLNIMSVVYLPQQDKDVALSMNWTNGAIEVLSIPDFEVIGEFNDLIKDKN
                     GNVKFVKYTDDALIIMNEDFSTYKSIPLPDKNTWSFQMVDYYSTCADEKIEFVFTGKE
                     TETGTGKGLLVINEDNETIIDMPDFSALHSSYYADNLPIILRKSTDKYGRPVSDEFIS
                     YYRTGKLPTQIKLGGNPLPGADMQILKQTDNELIALDKITMNADGKGEIELGEGVYYL
                     KALQTQNGLNTYYPNVLLWENATAIPYNTDPLEEGSMYCIEMQSIPVPVNPTAQGVIV
                     GKIVISQPEAVQQLMRIKLVTDNGSVPIFDVYLQNTADNSIIDHTQTDENGNYRFENV
                     PAGSYRVLVNVDGKPMESVNTVTLTTDADIVENVNYEITETGVVAKMPSGLKYTSTAL
                     IIYPNPAKDFIYLPQSAQNAKVQVLDVQGRILLNVDSYKGEKLDVSRLSNGWYMVQIN
                     NGNRIKTGKFIKIN"
     gene            complement(15968..17749)
                     /locus_tag="PLZ54_02690"
     CDS             complement(15968..17749)
                     /locus_tag="PLZ54_02690"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008776912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOJ65877.1"
                     /translation="MMQNIKERISALRNEMKINDIAACIIPSSDPHASEYIADYWKER
                     EWISGFNGSAGTVIITLTRAALWTDSRYFLQAENQLQGSGIELMKEGLPETLSWTDWL
                     GEELKNNEKVGLNAQMFSYNAFTHLKNELNKKGISLIAVDLIGKIWKDRPALPSQPFY
                     VYDIRYAGETASHKLASLREQMKKNQADIFVLSALDEIAWLFNIRGSDVDFNPVVIAY
                     AIIKIDKATLFVDSKKITEKTAAFLQEEGITIEPYNDIYSQLKKITEGKRVLIDGSKL
                     NAALYEAIPQHCSIINTMSPVLKMKSVKNKIELEGTRWAMVKDGVALVKFFKWLEENI
                     LSSEISEISAAEKLFECRQQQENFVSESFDTICGYAGHGAIVHYHAEPETDIVLKPDN
                     LLLVDSGGQYLDGTTDITRTIALGIPTQQQKIDYTLVLKGHIALAKAKFPTNTRGSQL
                     DILAHKWLWERGLNYGHGTGHGVGHFLCVHEGPQNIRMDENPAVLLPGMIISDEPGLY
                     RTNAYGIRIENLVLVVEDEKTEFGDFLCFETLTLFPYDRSLIELSMLTEDEINWINSY
                     QKKVLEKLSLYLNEEEKIWLQKKCASL"
     gene            complement(17961..18425)
                     /locus_tag="PLZ54_02695"
     CDS             complement(17961..18425)
                     /locus_tag="PLZ54_02695"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HOJ65878.1"
                     /translation="MKKQLKIALVAHDNRKADMVEWAVYNADMLSQHKLICTGTTGKL
                     IQRAMEEKGVKVDITCMHSGPLGGDAEIAALVVQKQINLAVFLIDDLNPQPHEADIQM
                     LLRQCRIHNVPIACNRYSADLMITSTLWDDENYIPTEPKYVKFVREDLFNFE"
     gene            18893..21214
                     /locus_tag="PLZ54_02700"
     CDS             18893..21214
                     /locus_tag="PLZ54_02700"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOJ65879.1"
                     /translation="MNLDFLNDLNESQRQAVEYMDGASLVIAGAGSGKTRVLTYKIAY
                     LLTKGFPPSSILALTFTNKAAREMKERIAQIVGERTARYLWMGTFHSIFSRILRNEAQ
                     YTGYSKNFTIYDSSDSSSLIKSILKERNLDTKTYPPGLIQSRISSAKNNLITPEAYAV
                     NSDFIKSDKQSRIPLLSEIYQTYCNRCKQADAMDFDDLLLQTNLLFRNHPEVLEKYQQ
                     LFQFILVDEYQDTNFAQYLIIKKLAQQHERISVVGDDAQSIYSFRGANIDNILQFKNA
                     YHNARIFKLEQNYRSTQTIVNAANSLIDKNRAQIQKTIFSEKEIGHPIKLLNVFTDYE
                     EGLTVAKHILDLYALNDYRYADIAVLYRTNSQSRILEETFRKQGIPYRIYGGLSFYQR
                     KEIKDVIAYCRLVCNQNDEEALKRIINFPTRGIGETTVGKLVECAQSNDVGIWEVLSN
                     MTKYGVMVNAGTASKLNDFRNLIESFSQQLEIKNAYDLAVTIVKTTGLLNEANFDHSP
                     ESISRKENIEEFLKAIHEFCEKKMEEGETLPLLPDFLSEVSLLTDQDTDKDSLSDKVT
                     LMTVHAAKGLEFKAVFIVGMEEELFPSMFSLVNERELEEERRLFYVAITRAKERCFIS
                     YAKSRFRNGKTNFCTPSRFIKDIDPQFLEITEEKAPTFQKKNFMNWDEEIESERNRFY
                     SPTNEKETYSEPQTINFSPRRLKKLDQSSDEKIIIENSSFPVGTIVKHQLFGIGKVLE
                     TSISNGNEKATIDFGEKGVKSLLLKFAKLEVVK"
     gene            complement(21320..22180)
                     /locus_tag="PLZ54_02705"
     CDS             complement(21320..22180)
                     /locus_tag="PLZ54_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOJ65880.1"
                     /translation="MRQLKITKSITNRESASLDKYLQEIGREDLISVEEEVELAQRIH
                     KGDRAALEKLTRANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAEKFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKINKAFSKFEQENERRPSPEELAE
                     ELDIPVDKIADTMKVSGRHISVDAPFVEGEDNSLLDVMVNEDSPDADHRLINESLSQE
                     IERALSTLTEREHEIIKKFFGIGVPEMTLEEIGDEFGLTRERVRQIKEKAIRRLRNSS
                     KSKLLKSYLG"
     gene            complement(22445..>23653)
                     /locus_tag="PLZ54_02710"
     CDS             complement(22445..>23653)
                     /locus_tag="PLZ54_02710"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOJ65881.1"
                     /translation="FFEFFFGRPRYYQEPPMVEGSGSGVIISNDGYIVTNNHVIDGSS
                     KIEVTLNDKRTFEAKVIGADPNTDLALIKIDAENLPIIPFGNSDSLKVGEWVLAVGNP
                     FNLNSTVTAGIVSAKARNINIINSKMRIESFIQTDAAVNPGNSGGALVNTRGELVGIN
                     TAIASQTGSFAGYAFAIPVSIVKKVVSDLRQYGVVQRAVLGVEIADITAQLAKEKDLK
                     TLEGAYVANVNAGSAAEKAGIKEGDVIVNVNGVAVKSVSELQEQIGRYRPGDKISITV
                     IRKNNELKFNNVELKNSQGSASIVSSDKVDVEQLLGATFKELSSKLQKDLGLDYGVQV
                     TSLTKGKLMQQGVRVGYIILKINNQTIRNVEDIQSAVTAALNSEDKVLFMAGVYPNGR
                     VTYYAVNLEE"
BASE COUNT         7570 a         4528 c         4293 g         7262 t
ORIGIN      
        1 attttttaca ggaaatattt ttaaaatttc ttttaaattc tatcgttaca ctgtttttac
       61 tctttctaca taagacccat cacgggtatc gactttaatt aattctcctt cgttaataaa
      121 aagtggaacc cgaattacag cacctgtttc cactgtggcc ggctttgtgg cattggttgc
      181 ggtatcgcct ttcaaacccg gttcggtata ggtaacttgc aaggtaacgt gagtgggaag
      241 atcaacataa agaattgttt cactttctgc atgggttacc acgtcacaaa tcattccttc
      301 tttcaaaaaa tctactccat tgattaattc cttatttacg gaaacttgtt caaatgtttc
      361 gttattcata aaattgaatc ccatatcgtc ctgataaaga tattgatagg gacggcgttc
      421 tatacgtact tcatcgattt ttactcccgc gttgaaagtc ttatctattg ttctgcctgt
      481 aactacattt ttcaattttg tacgtacaaa agcagctcct ttgccgggtt taacgtggag
      541 aaattccgta atgatataat actgtccatc aagttcgatg cacatgccgt ttttaatgtc
      601 tgcagtagta gccatagatt tttttattca aaggtaaatg aatgatttta tatattttga
      661 agataaccga tctgtttata aaaaaggttt gcaaatttac tacttttctc ttaattttca
      721 aaatgcttat aaaacactca ttgtcagata aataaaaaaa gagctgctca ttaaaatatg
      781 aacagccctt cgttaattta tggatgaatt ttatcaaaat tgtctgtcgg ccatttgttt
      841 atacatttta tagcgccatt tggcattatt ctcggctgca ataaacaatt cctgagcttc
      901 ggcagggaac tgtttcatca aggaagtata acgaatttcg cctttcagga aatcttggaa
      961 tttttcccat tgcggttctt tagaatcgag tataaaagga ttttttcctt ctgcttccaa
     1021 atcgggattg taacgataca gttgccaata accgcattca acagcgcgtt cctgttcctc
     1081 ctgagctttt tccattccgt tgtgaatgcc gtgactgata catggtgaat aagcaatgat
     1141 cagcgaaggc ccattgtaag cttctgcttc gcgaattgct ttgagcgttt gtgccttgct
     1201 ggctcccata gcaatttgag ctacatacac ataaccataa gttatggcca tcatgcccaa
     1261 atcttttttg cggattcttt ttccggcaga agcaaatttg gctatcgcgc caatgggcgt
     1321 tgattttgac gattgtccac cagtattgga ataaacttct gtatccaaaa ccaaaatatt
     1381 gacattttta ccggatgcta taacatgatc caatcctcca taaccgatgt cgtaagccca
     1441 accatcacca ccaataatcc attgcgattt ttttacgaga taatgggaaa gttccgaaat
     1501 tttccgacaa tgttcacaat cacaattttt gagaagcggc gtgattttgg cagccaattc
     1561 tttggttttt tcagcgtctt ctttgttttc aatccattcg gcaaaaagtg ctttcagctc
     1621 atcagaacag caattgcttt ctattgcttc tttcatgatt ctttcgagtc tgtcgcgcat
     1681 tacttcggtg gccaattcca ttccgtaacc gaattcggca ttatcttcaa acaacgaatt
     1741 tgcccacgct ggcccttgtc cattttcgtt ggtagtataa ggcgttgatg gagcagaagc
     1801 agaataaatg gaactacaac ccgttgcatt ggctaccaat tctctgtcgc caaagagctg
     1861 agttattaat tttacgtatg gcgtttctcc acaaccggca cacgctcctg aaaattcaaa
     1921 caatggtgtt gccaactgcg aattcttcac attcattttt acatccacca aatgctgttt
     1981 ggatttcact tccgagatac agaaatccca atttttctga ttgtcatatt gggtttctat
     2041 cggtaccatt gtaagcgcct tattgccttt tgttcctgga caaatatcgg cacaattttt
     2101 acagcccata cagtcgagaa catcaacttg aatgcggtaa gtcatgccca caaactgttt
     2161 cccgttagct tttatcgtat cggtgaaagg cgctttggcc tgttcggctt cgtccaaaac
     2221 aaaaggtcga atagcagcgt gaggacaaac gaatgcacac tcgttacact gaatacagtt
     2281 cgcagaattc caaacgggaa cgaaactagc tacgcctcgt ttttcatatt ttgcagttcc
     2341 ggaatcccaa gttccatctt cgcgtccctt gaaaacggag acaggcagat catcgccggc
     2401 ctgagcattt attggcatta ccacttcacg gatgaaagga ggaaagtttt cttccatttc
     2461 cttttcatct tccagatttg cccagttgct tggaacttca actttaacat attctcctcc
     2521 gcgatcaaca gcggcatagt tcatgtttac tatgtcttca ccttttttgc cgtaagtctt
     2581 gttaatagca tatttcattt tttcaactgc caattcgtaa ggaataacat ttgaaatttt
     2641 gaaaaatgct gattgaagaa tagtattggt tctgtttccc aatccaattt cctgagctat
     2701 tttggtagca ttgataatgt aaaaattaat gttgtgtgcg gccagatatt ttttaacttt
     2761 taccggaaga ttttttttca cttcttcttc gtcccaaatt gtattgagca ggaaagttcc
     2821 gtttttcttc aaacctttgg taacatcgta aagtctgaga taagcctgca catggcaagc
     2881 aacaaaatcg ggagtcgtta ccagataagt ggaacgaatt ggttcgtcgc caaagcgaag
     2941 atgagagcaa gtgaaaccac ctgatttttt agaatcgtaa gaaaaatagg cttgacaata
     3001 tttatcggta ttatctccaa ttattttaat tgaatttttg tttgcaccca ctgttccatc
     3061 cgaaccgata ccgtaaaatt ttgcttcaaa tattcctttt ccacccattg gaatttcttc
     3121 ccttagcgga agagaagtaa aggtaacatc atccactatg ccgacggtaa aatgattttt
     3181 tggttcggga agagccaaat tttcaaaaac ggccaaaacc tgagccggcg tgaaatcttt
     3241 ggaagaaagt ccgtatctac cacccacaac ggttggttga ttggatttag catacaatac
     3301 atcctttaca tcaagataaa gaggttcgcc ggaagctccc ggttctttgg tgcgatccaa
     3361 cacgcaaata cgttttgctg tttcgggtaa taccgacaga aaatgttttg ccgagaaagg
     3421 acgataaaga tgtacagaaa tcagtccgac tttttcgcct tttgcgttga gatagtccac
     3481 tccttctttg atggcttcag ttgccgatcc cattgcgata acaactctat cggcatccgg
     3541 agcgccataa taatcaaaca aaccatgttt tctgcctgta atctctccca atttgttcaa
     3601 ataatcttct acaatctccg gaactgcatc ataaaaagga ttggcagctt cacgagcctg
     3661 gaaataaata tcgggattct gagcggtacc tcttataacg ggtttctcag gattaagtgc
     3721 gcgattgcga aaagctttca aggcttcctg atcaattaat ggaattaaat cttcttgatc
     3781 caactgttca attttttgaa tttcgtgtga agtacggaat ccgtcaaaaa agtgaacaaa
     3841 aggaacgcgt gatttgatag ccgataaatg agccacagca gccatatcca taatttcctg
     3901 aaccgaacct gtcgcaaaca tggcgcagcc ggtttgtcga actgccatta catcctgatg
     3961 atcgccgaaa attgataaag cctgtgcggc aatagaacga gccgaaacat gaaaaacgcc
     4021 cggcaacagt tctccggaaa ttttgtacat attgggaatc atcagcagca atccttgcga
     4081 tgcagtgaaa gtagtagcca aagctcctgc ctgcaaagct ccgtgcattg ctccggcagc
     4141 tccggcttcc gattgcattt cctgtacttg aagtttttcg ttgaaaatat tttttcttcc
     4201 gtttgctgcc cattcatcaa catattctgc catagttgaa gaaggtgtga tgggataaat
     4261 ggctgccact tcgctaaaca tataagcgat atgggcggct gcctgatttc catcacaagt
     4321 tatgaatttt ttctgttttg ccattgtgtt gtgatttatt tgggttttta ctatagttta
     4381 ttatttggtt tagtataaaa aaccgaagag ccaaattgga atttcattct ttttcccaat
     4441 tttcagttga tcggttgcaa aaattaattt tgaatgttgt ttggaaatat ttgaatttaa
     4501 ctcgcattta aacgtatatt gtttatcaac agtaaaatcg gaatgatatt ttccggagtt
     4561 aactttgtat tttgcatgca gcgaattgta caaaaaggtt tttatttctg ataatctgtc
     4621 gatatttccc ggcatagcgg catgcattaa gtttgtattg tgaacatata tcaaatgagg
     4681 ttttttagga aattgttcat tgtcgttata aagcaagttc aaaagtcggg catctttgag
     4741 atactttatg taattcataa tagttgctct cgaagtttga atttcgcaac tcaactggct
     4801 tatgttcgga gttgcagggg cactgatcat gagtaaataa agcaattttc tgatttttga
     4861 aagatatttc aattcgattt gtttgatcag aagaatatca acttcaatca tcatattcat
     4921 ggttttcagc agattttccg aaaaatttct ctgttccaaa aaaaacggat aataaccatg
     4981 atgcagataa tcttgaaaat aaagcaaagg atcaatttta ctgcaaattt catttgctat
     5041 cggcacatga ttttttataa tatcttccaa atcaataggt tgaaagtttg tcccggcttg
     5101 caaattcaaa aattccctaa aggaaaaacc tcttagatta tatactttta caacgtcttg
     5161 taaatcttca ttgtcttctt ttaaacgcat tactgacgat ccgctgaaaa ttattttcag
     5221 ttcggggaaa cgttcataac attcacgcaa ttcctttgac caattttcgt atttgaaagt
     5281 ttgatcaatc aacaaagtac gtccgccctg tttcacaaaa cgctctgcaa attctaccaa
     5341 agtatgggta gtaaagtaca aattgttgaa attgatatat aaacaacttt gatcattcga
     5401 cctgaaaaaa tcttttgcat attgcagcaa aaaagtagtc tttccaactc ctctacttcc
     5461 ctttatccct atcaaccgat cggtccagtc tatttcatcc atcaaagttc gtctcacagg
     5521 agattgtaca tgttcaacca aatattgatg cgttctgaaa aatgactcca tcacgtataa
     5581 attttgtttg caaaaatagc aatatttatc tgattttgca aagtggagat gacaatttta
     5641 gcctttttat ttttattggt taagttttgg gttaagaaaa ctttaataaa atttattttt
     5701 ttagttggtt agggtttaaa tgtataattg ttgataaaaa attggataga aatttctgtc
     5761 aataacaata atcatttact tttgaaaaat aaaaggctct tttttatcaa cagaaatttt
     5821 ttaagtaaac ttaaaaaatt ccaaaaaaag gattaaaaac caaagattaa aatcattaat
     5881 tatatgaaaa tcaagtttat aggagcagca cgtgaagtta caggcagcaa acatttaata
     5941 accacaaagg atggaaaacg gatattgctc gattgtggaa tgtttcaggg aaaaggattg
     6001 gaaaccgatg ccaaaaataa agatttgggt ttcgatcccg aaacaataga tcatattatt
     6061 ctttcacatg ctcatattga tcattccgga ctgattccct atctctatcg aaatggtttt
     6121 cgtgggtcgg tcatttgtac caatgcaacc cgtgatcttt gttccattat gttagccgat
     6181 agcgctcata ttcaggagct tgatattaaa tggtttaata aaaaacgcgc acgtcagggt
     6241 ttgccgccag tggagcccat ttatgataag gaagatgcgg aaaattgcat ggatttgttt
     6301 attggagttt cactcaatcg ccgtttttat attgacaata aaattaatgt gaaatttacc
     6361 aataccggac atttattggg aagtgcagtt gtgaatctcg aaataattga aaatggaaaa
     6421 aaagttcaca ttgcctatac cggggatatt ggtcgttcaa ccgattactt gttgcgttca
     6481 ccggatccct ttcctcaatg tgattacctg attgccgaat caacttatgg caaccgtctt
     6541 catcctgaaa caaatcaaac ggaagaagat tttcttaaaa ttataactga aacctgtatc
     6601 aaaaataagg gaaaattgat cattccagcc ttttctattg gacgaaccca ggaattggta
     6661 tttctgttga ataattttta taatcgacat ttacttcctc acattgaagt ttttgttgac
     6721 agtcctctat ctgtgaatgc tacagaaatt tatcgaatgt acagcgaact aatgaataag
     6781 aatgtgaaag aagtgctcaa atacgacaat gacccatttg gattcaattc cttacactac
     6841 ataaaaaatg tggaagaatc aaaagcttta aatacctatg acaaaccctg tataattatt
     6901 tctgcctcgg gtatgatgga agcaggacgt atcaaacatc acgtggccaa taatattgaa
     6961 aatccgcgaa atacgatctt aattgtaggt tattgttcgc ccacttcttt aggtgctcgc
     7021 attcagcaac ctggcttaag ggaaatatcg atatttggag aaattcatcc cgttaatgcc
     7081 aaaatttgca aaattgaggc attttcaggt catggcgatt ataaagaaat gattgattat
     7141 ctgggttgcc aatcagcagg cttgattaaa aaaacttttc tagtgcacgg ggattatgaa
     7201 gttcaggtag aatatcgtga aaaattacaa aaagaaggtt tcaggaaagt tgagatacct
     7261 gatgcaaagg aggaatttat actgtaggaa aatttttaaa gttgaaaacc taacaagtta
     7321 acttaatcca aattaattga ctatcataaa tttaagtggc tatgaagcca ctacaaatac
     7381 tcctttaatt gttaaatgtg cctgtttatt agttcttcta agtttttata gaaatgttta
     7441 aatagatgaa ttaacaaaat gaattaacag ggaaaacata aatatttgaa acacattttt
     7501 ttaaccacct taaattaaaa attcaatttt ttccgtgaaa ttttcaaaaa aagtttcttc
     7561 atattaaaag aatatcctaa atttagagcg caatatatca aagtaaaaat ttataaacat
     7621 ctaatactta attgtcatga caaaaatgat aacattcaac gaattgcgta gaattaaaga
     7681 tgcgcttcca catgggagcc tgagtcagat cgccgaagaa cttgggttgg atattgaaac
     7741 agtacgaaat tactttggcg gtcataatta caaagatggc aaaagttgcg gaattcatat
     7801 tgaaccgggc cctgatggcg gtttagtcat gttggataac acagatattc ttgaggctgc
     7861 tttacgaatt ttggggaaag aagaagctta aaagtgcaaa aggaaaagga aaaaagaaga
     7921 aaaagaaatt gaaaggcagt gaaccagcat tcgctgcttt tttttaatcc gatgcttaaa
     7981 aaatcattcg tccaaaaatc gtcccattat ctataatgta ttcatttttc atcattttaa
     8041 gcaatatttc ctatatcatt ataaaactcc ctgtcttatt ttcacaaaaa agactaattt
     8101 tgcaacatac aaccaacaat ttcatttcgc atgaatcttt cgccggcttt tgaaaaaaga
     8161 atgcaagttt tattggaaga ggaatacgct ttgttcagag cagcattaga aaaaccggct
     8221 ccggtaagta tcagagcaaa caacaaaatt gtttaccaac cttccaaaga acaagtgtca
     8281 tggtgtgaaa gtggatttta tctgagcgaa cgcccggttt ttacagttga tccgtttttt
     8341 catgctggcg tatattacgt tcaggaagcc agttctatgt ttctccgtca atgcctgaaa
     8401 caatattttt cacaggcaga aacagttttg gatttatgcg ctgctccggg cggaaaaaca
     8461 acgctttttt cacaatatat aaatgaaaat gctctgttag tcagtaacga aattaatcgt
     8521 tcgcgagctt atattctggc cgaaaatgtt gcaaaatggg gaaatgccaa tgtggtggtt
     8581 accaataatc aacccgctga ttttaaaaat cttcccggct ttttcgacgc catgttaata
     8641 gatgcaccct gttcgggaga aggaatgttc agaaaaaatc ccgcttcgat tgatgaatgg
     8701 tcgttgacaa atgttgaaaa ttgcgtcgtt cgacaacgga aaattatctt cgaagtctgg
     8761 gatactttaa aaaccgacgg tattcttgtt tatagcacct gtacttacaa ccgtcaggaa
     8821 aacgatgaaa acatcaaatg gatttgcgaa gaactcggcg ctgaaatttt gagaatggat
     8881 attcaacatt ttcaaggcat tctggaaacg gaagccggtt atcgtttttt tccacataaa
     8941 ataaaggggg aaggtttttt catttccgtt ctacgcaaaa cttctcctac accgcaaaaa
     9001 atgaggaaag caaaaaaaat tgaaagaaag tcggatgaca aaataaagtc cgaagtcgga
     9061 ataaaacttc ttgaaccgga aaaatggaat ttgttcatgg aaaacaatcg tctccgcgca
     9121 gtaagcaaag ccaggcaaaa agaaattgct atcattgaaa ataatttaaa atgcctgctt
     9181 tcaggtattt atttgggcga ttataaaaat tccgatttta tcccttccgc tcattccgct
     9241 ttatccaaag aaaccgattt gaaagcagtc gaaacggttg aaattgacta tacaactgcc
     9301 gttcgttatt taaagaaaga aaacattcaa gttgaatctg acaaaaaagg ttttttattg
     9361 cttctttata aaggtgttcc tttgggatgg gttaaaaata taggacagcg atgcaacaac
     9421 ctttatcctc aggaatggag aataagaatg aaaattgaag aattagaaga aatatgaaaa
     9481 ttttgttatt aggcaccggc acttctacag gcgtacctca aattggttgc aactgctata
     9541 cctgcacttc caccaacatt aaagatcaac ggctgcgttc ttccttgctg gtaagcgaaa
     9601 aaaataccag aattttgatt gactgcgggc ccgatttacg acagcaatta ttgaattatc
     9661 gcattcacga tttaaccggt attctgctta cccacgaaca ttacgaccac attggcggac
     9721 ttgacgatct tcgtccgctt ggtccttcgc atttgtttgg cgaaaaaaga gttttgaaag
     9781 ttattgaacg aaatatgcct tactgttttg gcgataaacg ttatccgggt gtacccgaat
     9841 tgtttcttca tgaaattaac gaagaagaat tttcacttga tggtttaaaa gttcaacctg
     9901 ttcgtgtcat gcatgccaat ttgcctattt ttggattccg aatcgggaaa atggcttacc
     9961 ttaccgacgt caaaacgctg gatgaaaaag ctgttgaaca acttcaacaa ttggatgttt
    10021 taataataaa tgccttgcga ccttttaaac atatttctca tttatctttg tcggaagcca
    10081 tcgaattggc agaaaaaatt aatgccaaag aaactttttt tacccatatc agccacgata
    10141 tgggacgaca taacgaaata aattcggtac ttccgcccca tattcatttg gcttacgatg
    10201 gattggagct tgcaatttga gacagagaga aattcagaga aattattaaa attatcttct
    10261 cgcaaactaa atgcgcacat tttcgtttga tttatgaaaa aaataattaa agccagatga
    10321 aaaaaagttt tataatattt gttggtttat ccattggggc catgttgttt agttcctgtt
    10381 caaacatgct ctatacctcg ctcgacgtac tcagaccggc taaagttgct tttttgccca
    10441 atgttaaccg gcttttgata gtaaacaaca cacctgtcat ggcattggaa aacgatccga
    10501 acaaaaccaa gaaaaatgtt acgcttccag ccgacagttt gccaatttac tgcttaagcg
    10561 tattggctca ggaacttggt aacaacagct ttttttcatc cgttctgttg gaacctaact
    10621 ccatttatcc aaaaaacaat tattccgaaa agctttcaac cgaaatggta aaaaaacttt
    10681 gtgaaaatta caactgtgat gtaattctga cattggacag tctcaaagct tcggatgtgt
    10741 tgacagaata ttatctcgaa gacattagcc agtatttaag tacgtatgaa atttacctca
    10801 aatcaaaatg gagtatttac tatccttcgc aatccaaacc ggtttcttta cagtttaaag
    10861 atacagtata ttgggaagct caggattatt cgcggcagag agctttgaag caaatgcccg
    10921 acagatggga tgcattggtt gatggcgctt tgtatgtagg aaaaaattcg gtaaaaagat
    10981 ttattcctta ttgggataaa gttgatcgtt attttttcaa tcccggcaac aagttaatgc
    11041 gacaggcaat ggattctgtt tatgtaagaa attggacttc ggccattgaa ttgtggaaaa
    11101 aagccatgaa caaatccaaa agcgaaaaac tcaaaggtga agctgccaac aatattgcca
    11161 tcgcctatga aattcttggc gatatggata atgccattgc gtatgccagc cagtctcttc
    11221 aatatttttc ttctgtttcc attattgatt attacacact ggaaagaatc aacaactatt
    11281 actacgaact gataaaacga aaaaatgaaa ttcaacaact gaaattgcag ttgggagaat
    11341 gatttttttg agcaatggag caacaatcat aaaaatcaat tcaacttatt gataaccaac
    11401 aaaaaacgaa aaaatatgga actacttttg ccttctgatg aaattttatt acaggaaaaa
    11461 ggaatcggca aacagcaaat cgaagaacaa ttgcattatt ttgaaacagg attccctttt
    11521 ttgaaaattg ttgcaccggc cgtaattggc aacggaatta caattatcga tgataaaaaa
    11581 ggagaatttt acattaacaa atgggaaagt tacagacaaa aagaaggaat cgatatagtt
    11641 aaatttgttc ctgcctctgg agcggcatcc cgaatgttta aagatttgta cgaatttttg
    11701 gaaagtgaag cagaattacc tcaaaaatct tctgtaaaag attttttcaa tcaaattgaa
    11761 aaatttgcct tttacgaaga gttaaacaaa gcctgcctga aaaatgaggg aaaaagtgtt
    11821 gccgaattga taaaaaacaa cagacaaaaa acggtagtag aaaatctgtt gcttgaaaaa
    11881 ggtttgaatt acggctttct accgaaaggt ttgttgcttt ttcattccta tcccgacgaa
    11941 aaaaggactc cggttcagga acatttggtt gaaggagctt tgtatgctac ccatgccgac
    12001 aacgatgtaa aaatccattt tacagtttca catcaacaca gagctttatt tgaaaaacac
    12061 attcaggaaa ttctggaaaa atatgagcaa aaattcaaag tacattatca tatcgacttt
    12121 tctgaacaaa aacccagtac cgacacattg gctgtagata tgaacaatcg gcctttcaga
    12181 gaaaacgggc aactggtatt tcgtccggga ggtcatggcg ctttactgga aaacctgaac
    12241 gacctacttg ccgatatcat tttcataaaa aacatcgaca atgtggtgcc ggattccttt
    12301 aaagaaatta ccgtcaaata caaaaaaatt atagcgggag ttttaattga tattcaacaa
    12361 ctgtgcttcg attatttaaa agaactggaa aacaataatt taaccgaaaa tcggttgaaa
    12421 gaaatacttc gattcacaga agaaactctt tgtattcgtc atcccgaaat cgattctttt
    12481 gacaccgaag cattaaaaaa atatctgttc cgtaaattaa atcgacctgt gagggtctgc
    12541 ggaatggtaa aaaacacggg agaacccggc ggtggtccat tcctcaccgt aaatgccgac
    12601 ggaacaattt ctccgcaaat tttggaaagt tcacaaatcg acctgaacaa tccggaagag
    12661 aaacaaaagt tggaaaattc tacacatttc aatccggtag atttggtttg ctcggtaaaa
    12721 aattacaagg gagaaaaatt tgatttaacg aaatatgtcg ataaaaatac cggttttatc
    12781 tccattaagt caaaaaacgg aagggaactg aaagcgctgg aacttccggg tttgtggaac
    12841 ggttcaatga gtgattggaa tactgttttt gtagaagttc cgattgaaac ttttaatccg
    12901 gtgaaaaccg tcaacgattt gctacgccct caacatcaat aaaaaaccaa taaggatact
    12961 aaaaaaccgg ttatcaataa aatttgataa tcggtttttt ttgtgcggct gaagggactc
    13021 gaacccccac gcctttcggc accagatcct aagtctggcg cggctaccaa ttacgccaca
    13081 accgcataag gattattggc tgcgaagata aggaaatttt caggatatta caagttttta
    13141 cggtttgtaa aggaaatgat ttcctgagaa ttaattaata aattttattc atttatctga
    13201 taatcaaagt attaatttaa tttgcaacca agatgtattg tctgtttgat tgacgggaga
    13261 gaaaaatcga tatgaaaaat ttgaattaaa atcgagcgta aacaagattt ttacgatttg
    13321 cctgaaaatt tttaccaaat gcttacctat cgcattaagt tttgactatt tttgtttcaa
    13381 actattttag atacactaac ttaaattaat aatgctatga aaacaaaact acttttatca
    13441 ttgggtttat tgggaacaat gtcctttttg tatggtcagc aatacagacc cggaaaacaa
    13501 atagaccatg ataacactgc ttttgtacga aaagtcgaat taaaaaaagc gggagaagtc
    13561 attatgaaag tcgatcaaat ttctgttacc gatacaactt ctgagctttc ggcatcgatt
    13621 tatttacctg attttacact ttttaaaaat tttcatgctc ccgttccggc tgacggagca
    13681 caatttttct tcggacacgg aggcgtaact gtggctgaaa acttggtaaa caatcagcct
    13741 cgttattatt ttctttatca aacagaagga tatagtgaag acgagtccgg ttttagattt
    13801 tttcaagaca ttcatatcat agacgaaaat cagcagcaag tggggaaagt gccaacttcg
    13861 tggatgggtt atcaactcta tatgcaagat ggtaaaaaca tgctgatatc cgactatcag
    13921 gactgggata cgatattttg gagcaatcct gaaaccggat ggtcgggttg ggagactttt
    13981 caggtgcata cctctgttgt tactgatgta gcaacagggc aaaccatttt tactttccca
    14041 cgaggcacta ctttctcgaa cttcaacgga atgcaatcgg ggatatacta tgcaacaaat
    14101 gagcaaaatc tgtttttcaa tattgatgga gaagaaagaa tactcactac caataacaat
    14161 tttgaaaaat attttaaagg acattcctca ggaaatcatg aaaatgccct tgacatgtgg
    14221 atttacaaca aagatttttc attgtataag aaaatttcaa atgtgggata tgatattccc
    14281 atcgaaaact tatatatggc caatacctat caaccacttt tcggaaaaca tgctgcttac
    14341 ttttatcaca cggttaccta tcaaacagat ggaggcttaa attacaacag taaaacatac
    14401 ctggtaaatc tgaatggaga gttggttaaa attatcgacg gaaatctcaa tatcatgagc
    14461 gtggtatatt taccacaaca ggacaaagac gtggcattga gcatgaattg gacaaatggg
    14521 gcaatagaag ttttaagtat tcccgacttt gaagtaattg gagaatttaa cgatttgatt
    14581 aaagacaaga atggaaatgt aaaatttgta aaatataccg acgatgcatt gataatcatg
    14641 aacgaagatt ttagtactta caaatctatt cccttgcctg acaaaaacac atggagtttc
    14701 caaatggtag attattattc aacgtgtgca gacgaaaaaa tagaatttgt atttaccgga
    14761 aaagaaactg aaactggaac tggaaaagga ttgctggtta tcaatgaaga taacgaaacc
    14821 attattgata tgccagattt tagcgcactc cattcttctt attatgctga taacttgcct
    14881 attattttaa gaaaatcaac agacaaatat ggtcgtccgg tatcggatga atttatctcc
    14941 tactatcgca caggcaagtt gccaacccaa atcaaacttg gtggcaatcc attgccagga
    15001 gccgatatgc aaatactgaa acaaaccgat aatgagctta ttgcactcga taaaattacg
    15061 atgaatgctg acggaaaagg agaaattgaa ttgggtgaag gtgtttatta cttgaaggca
    15121 ctgcaaaccc aaaacggatt gaatacttac tatcccaacg tgctgttatg ggaaaatgca
    15181 actgccatac cttacaatac cgatccgctt gaagaaggaa gtatgtattg cattgagatg
    15241 caatctattc cggttcctgt aaatccaaca gcacaaggag ttattgttgg taaaattgtt
    15301 atcagtcaac cggaagcagt tcagcagttg atgcgaataa aactggttac cgacaatggt
    15361 tctgttccaa ttttcgatgt ttatttgcaa aacacagccg acaattccat tattgaccat
    15421 acacaaaccg atgaaaatgg aaattatcgt ttcgaaaatg tgccggcagg ttcgtatcga
    15481 gttttggtga atgttgacgg gaaaccaatg gaatccgtca atacagtaac attgacaacc
    15541 gatgccgaca ttgttgaaaa tgtaaattat gaaattaccg aaaccggtgt tgttgccaaa
    15601 atgccttccg gattgaaata tacttcaacg gcgctgataa tctatccgaa tccggccaaa
    15661 gatttcatat atcttccaca gtcggcacaa aatgccaaag ttcaagtttt ggatgtgcaa
    15721 ggaagaattt tattgaatgt cgacagttat aaaggagaaa agcttgatgt gtcaagactt
    15781 tcaaatggtt ggtacatggt tcaaataaat aatgggaaca gaataaaaac aggtaaattc
    15841 attaaaatca actagcgctt aaaacaacta tactgaaaaa aatatctggc ttacagcaat
    15901 ataagtcaga tatttttata aaattcaatt tgcaaacaga ttaaacaata ctcttttttt
    15961 aaagaaatta caaagaagca cattttttct gcagccaaat cttctcttct tcattcaaat
    16021 aaagagaaag cttttccaaa acttttttct gataagaatt tatccaattt atttcgtcct
    16081 cagtgagcat ggaaagttca atcagcgacc gatcgtaagg aaaaagtgtg agtgtttcga
    16141 aacacagaaa atcaccaaac tcagtttttt cgtcttccac gaccagcacg agattttcaa
    16201 tgcgtatgcc ataagcattg gttcgataaa gacccggctc gtcggaaata atcattcccg
    16261 gcaaaagtac ggcaggattt tcatccatcc gtatattttg tggcccttcg tgaacacaaa
    16321 gaaaatgtcc aacgccatgt cctgttccgt gcccataatt caaacccctt tcccagagcc
    16381 atttgtgcgc aagaatgtcg agctgggatc cacgcgtatt ggtagggaat ttagcttttg
    16441 caagtgcaat atgacctttc aatacaagcg tataatctat tttttgttgc tgagtgggaa
    16501 ttcccaacgc tatggttcgg gtaatatcgg tagttccatc caaatattga ccacccgaat
    16561 cgacaagtaa gagattatca ggctttaaaa cgatatcggt ttcaggttcg gcatgataat
    16621 gaacaatagc cccatgacca gcataaccgc aaatggtatc gaaactttcc gaaacaaaat
    16681 tttcctgttg ttgcctgcat tcgaacaact tttctgcagc ggaaatttcg ctgatttctg
    16741 aagataaaat attttcttcc agccacttga aaaattttac cagcgcaaca ccatctttta
    16801 ccattgccca tcgagtacct tccaattcta ttttattttt tacactcttc attttcaaaa
    16861 ccggcgacat agtattgata atgctgcaat gttgcggaat agcttcatac aacgcagcat
    16921 tcaacttgga gccatcaatc aaaactcgtt ttccttcagt aatttttttc aattgggaat
    16981 agatgtcgtt gtacggctct atggttatgc cctcctcttg aagaaaggca gcggtttttt
    17041 ccgtaatttt tttcgaatcg acaaaaaggg tagccttatc gatttttatt attgcataag
    17101 ctattaccac cggattaaaa tccacatcgc tccctctaat gttgaacaac caagcaattt
    17161 catccaaagc cgaaagcaca aaaatatctg cctgattttt tttcatctgt tcgcgcaggg
    17221 aggccagttt atggcttgcc gtttcacccg cataacgaat atcgtaaaca taaaaaggct
    17281 gagaaggcaa agcaggtcgg tctttccaaa ttttaccaat caaatcgaca gcaatcagtg
    17341 aaattccctt cttgttcaat tcgtttttca gatgggtaaa agcattgtaa gaaaacattt
    17401 gggcatttag tcccactttt tcattatttt tcagttcttc tcccaaccag tcagtccagc
    17461 tcaaagtttc cggaagtcct tccttcataa gctctatgcc ggagccttgc aactgatttt
    17521 ctgcctgtaa aaaatatcgc gaatcggtcc acaacgcagc tcttgtcaaa gtaattatca
    17581 ctgtaccagc cgaaccgtta aatcccgaaa tccattcgcg ctctttccaa taatcggcaa
    17641 tatattcgct ggcatgagga tcgctactcg gaataataca ggcagctata tcatttattt
    17701 tcatctcgtt gcgcaatgcc gagattcttt cttttatatt ttgcatcagt attcatttta
    17761 atttttccct gttgatgcgg aaaattttta tattaacatt ttaaaaatga aataattatc
    17821 tgaaagtaaa aaaattattg ttaagtactt tcatcagaat ataatttatt ttttaaaggt
    17881 ctgatggaac ccacatgtct gaatattata ttcattcgtg tttgtgtatg caatacatgt
    17941 gggtttcata aattaaaatt tcattcaaag ttaaataaat cttctctaac aaatttcaca
    18001 tatttgggtt cagtcgggat gtaattttca tcgtcccaca gcgtgctggt aatcatcaaa
    18061 tcggcactgt aacgattgca ggcaatggga acattatgaa tcctgcactg acggagcaac
    18121 atttggatat cggcttcgtg tggctgtgga ttcaaatcat caatcagaaa aactgccaga
    18181 ttaatctgtt tttgtaccac caatgcggca atttccgcgt cgccgcccaa agggccggag
    18241 tgcatgcaag taatatccac cttcacacct ttttcttcca tagccctttg aatgagtttt
    18301 cctgtcgtac cggtacaaat caatttgtgt tgcgaaagca tatcggcatt gtaaacagcc
    18361 cattctacca tgtcggcttt tcgattatca tgagctacca gcgcaatttt caattgtttt
    18421 ttcataagtt ttatgttttt tgatgaaaga atttattaaa gttttcaatg aaaatctcag
    18481 ttgaaggttg aatcattttt tcgattcaga atcgatcgaa ttaatgaaaa ataaaaaatt
    18541 caatctataa aaacaggaat ttcaaacatt aatagaaata aattttttgc tgttttcgaa
    18601 tgttttttca gaaaaaacct tataatccta atggttgcaa aaataaagaa taaatattta
    18661 tttcggacaa ggaaaatgaa aaaactttta taataacatc atagcgcata aaactcattt
    18721 gattatttca agctgtttat atctcgttaa atttttaaac aaagctttca accgaaaagt
    18781 acttatggtt tcataaaaat tgtctttaaa aaccgattca atttgctaac tttgcataaa
    18841 gaaaaccggt aagatttaaa gtttctacaa aaatatttgg atgaacaaat caatgaattt
    18901 ggatttttta aacgatttga acgaaagtca gcggcaagct gtagaatata tggatggcgc
    18961 ttcgctggta atagcgggtg cagggtcggg aaaaacccgc gtgttaactt acaaaattgc
    19021 ctatttactc accaaaggtt ttcctccgtc gtccattttg gctttaactt tcaccaacaa
    19081 agcggcacga gaaatgaagg aacggattgc acaaattgtt ggagaaagaa cagcccgata
    19141 cttgtggatg ggaacttttc actccatttt ttccaggatt ttgcgtaacg aagcacagta
    19201 caccggatat tcaaaaaatt tcaccatata cgactcttcc gattcttcca gcttgataaa
    19261 aagcattctg aaagaacgca atctggatac aaaaacttat ccgcccggac tcattcaatc
    19321 acgcatttct tcggcaaaaa acaacctgat tacacccgaa gcctatgccg tcaacagcga
    19381 ttttattaaa agtgacaagc aaagccgcat tccgcttttg agcgaaattt atcaaactta
    19441 ttgcaatcgt tgcaaacaag ccgatgcaat ggatttcgac gatttactgc tccaaactaa
    19501 tctgctgttt cgaaatcatc ctgaagtgct ggaaaaatat caacaattgt ttcaatttat
    19561 tttggttgac gagtatcagg ataccaactt tgcgcaatac ctgattataa aaaaacttgc
    19621 ccagcaacac gaacgaatta gtgttgtggg tgacgatgca caaagcattt attcatttcg
    19681 cggtgccaac atcgacaaca ttctacagtt caagaatgct tatcacaatg cacgcatctt
    19741 caaactcgaa cagaattatc gttccacaca aaccatcgtc aatgcagcca acagcctgat
    19801 tgacaaaaac agggcacaaa ttcaaaaaac cattttttcg gaaaaagaaa tcggacatcc
    19861 cataaaattg ttgaatgttt tcacagatta tgaagaaggt ctgaccgttg ccaaacatat
    19921 tttggacttg tacgcgctaa acgattatcg atatgccgac attgctgtgt tgtatcgaac
    19981 caattcacaa tcgcgaattt tagaagaaac attccgaaaa cagggcattc cctatcgaat
    20041 ttacggaggc ctgtcgtttt atcagcgaaa ggaaataaag gatgtgatag cttattgccg
    20101 attggtatgc aaccaaaatg atgaagaagc attgaaaaga atcattaatt ttcccacgcg
    20161 aggcattggc gaaactacag taggaaaact tgtggaatgt gcacagagta atgatgtagg
    20221 aatttgggaa gttctttcca acatgacaaa atacggcgtg atggtcaatg ccggaacagc
    20281 ttcaaaatta aacgatttcc gaaatttgat agaaagtttt tcccagcaac tggaaattaa
    20341 aaatgcttac gacttggccg ttacgattgt aaaaaccacc ggattgctca acgaagccaa
    20401 ctttgatcat tctcctgaaa gtataagcag aaaagaaaat atagaagaat ttctgaaagc
    20461 catccacgaa ttttgtgaaa aaaaaatgga agaaggagaa accttgcccc ttcttcccga
    20521 ttttttatcg gaagtatctt tattgaccga ccaggacacc gacaaggaca gcctgagcga
    20581 caaagttacc ctcatgaccg ttcatgcagc caaaggattg gaatttaagg ccgtattcat
    20641 tgttggtatg gaagaagaac ttttcccgtc aatgttcagc cttgtcaatg agcgggaact
    20701 agaagaagag cgccgccttt tttacgttgc cattacccgt gccaaagaac gctgttttat
    20761 cagctatgcc aaatcgagat ttagaaacgg taaaaccaac ttttgcactc ccagcagatt
    20821 tatcaaagat atcgaccctc agtttctgga aattaccgaa gaaaaagcac caacttttca
    20881 gaaaaaaaat tttatgaatt gggatgagga aattgaaagt gaacggaatc gtttttactc
    20941 gccaacaaac gaaaaagaaa cttattcgga acctcaaaca ataaattttt ctccacgaag
    21001 actgaaaaaa ttggatcaat cgtcggacga aaaaatcatt atcgaaaatt cttcttttcc
    21061 tgtaggaact attgtaaaac atcagctttt cggaattgga aaagtattgg aaaccagcat
    21121 ttccaatggt aacgaaaaag caacgatcga ttttggagaa aaaggagtaa aatcgctttt
    21181 actgaaattc gcaaaactgg aagtggtgaa ataattatcg aagagtattt actgatgcct
    21241 taatgggtta aggaataaaa aaagcgtcct ttttggggac gccctttgca ttcattttaa
    21301 aataagcatt ttatgaaaat tacccgaggt aactcttcaa taatttactt ttagaactgt
    21361 ttctcaatct acggatagct ttttccttga tttgacgaac acgctcgcgt gtcagtccaa
    21421 attcatcgcc gatttcttcc aacgtcattt caggtacgcc aatcccaaag aattttttaa
    21481 taatttcatg ctctctttct gttaatgttg acagagccct ttcaatttcc tgggaaagtg
    21541 attcattgat caaacgatgg tcggcatcgg gagaatcttc atttaccatc acatcgagca
    21601 ggctgttgtc ttccccttca acaaaaggcg catctacgga aatgtgacga cctgaaacct
    21661 tcatggtatc ggcaatttta tccaccggaa tatcgagttc ttcagccaat tcctcaggtg
    21721 aaggacgacg ctcattttcc tgttcaaatt ttgaaaaagc cttgttgatt ttgttcaacg
    21781 aacctacttg gtttaatggc aaacgaacaa tacgcgactg ctcggccaac gcctgcaaaa
    21841 tggattgcct gatccaccac actgcatagg aaataaactt aaatccgcgt gtttcatcga
    21901 atttttcggc agccttgata agtccaagat ttccttcatt gatcaaatcg ggcaaactta
    21961 agccctgatt ttgatactgt tttgctaccg aaacgacaaa acgcaaattg gcgcgggtta
    22021 atttttccaa agcggctcga tctcccttgt gaatccgttg agccaactca acttcttctt
    22081 ccacactgat cagatcttct cttccaattt cttgcaagta cttgtcgagc gatgcgcttt
    22141 cgcgattcgt gattgatttt gtaattttta attgcctcat ttttacctta aaaaattagg
    22201 gtgcaaaatt aatgtttttt tggttgatta caaaatttct tatataacgt ttttactaaa
    22261 tttttgtttt aaaaagtctt gaaaattttt ctttgttgtt ccataaatac gccgaaacga
    22321 tattttatga gttgcctgat tgttgataat ctcaaatttt agtacaaaag gaacttactc
    22381 ctttcgtaaa aaagagtaag ttccaatgca acagagaaat atttttcaac acacttatgg
    22441 aaaattattc ttccaaatta actgcatagt aggtaactct gccattggga taaacacccg
    22501 ccatgaaaag caccttgtct tcgctgttta atgccgcagt tacggcagat tgaatatcct
    22561 ccacatttct aatagtctga ttgttaattt ttaatataat atatccaact ctcacacctt
    22621 gctgcatcaa ttttcctttt gttagcgatg taacctgaac accataatcc aaaccaagat
    22681 ctttttgcaa cttactgctc aattccttga aagtagcacc cagaagttgt tcaacgtcaa
    22741 ctttgtcgga agaaactatg ctggcacttc cctgactatt tttcagttct acgttgttaa
    22801 atttcaattc attattttta cgtataacgg tgattgaaat tttatcgccg gggcgatagc
    22861 gtccaatttg ttcctgcaat tccgaaacag atttaacagc cacgccattc acattcacaa
    22921 ttacatcacc ttctttaatg cctgcttttt cggcagcact tccggcattt acattggcca
    22981 cataagctcc ttccaatgtt ttcaaatctt tttcttttgc caactgtgcg gtaatatcag
    23041 ctatttcaac tcccaacacg gctcgttgta ctactccgta ctgacgcaaa tcggaaacta
    23101 cttttttaac tatgctgacc gggatggcaa atgcgtaacc tgcaaacgaa cccgtctgag
    23161 aagctattgc cgtattgata cctaccaatt cgccccgtgt attgaccaaa gcgcctccac
    23221 tattacctgg attaaccgca gcatcggtct gaataaaaga ttcgatacgc attttggagt
    23281 taatgatatt gatatttctt gcctttgcac tcacaattcc cgccgtaacg gtagaattaa
    23341 gattgaaagg attccccact gccagcaccc attcgccaac ttttagcgaa tcggaattac
    23401 caaaaggaat gatgggaaga ttttctgcat cgatttttat cagcgccaag tctgtattag
    23461 gatcggcacc aattactttt gcctcaaatg ttcgtttatc gtttaatgta acttctattt
    23521 tggacgaacc gtcaattaca tggttgttgg ttacaatata tccatcattg gaaattatga
    23581 cgccagagcc agaaccttct accataggcg gttcttgata atagcgagga cggccaaaga
    23641 aaaattcaaa aaa
//
LOCUS       DAOSCH010000018        22824 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1627_length_22824_cov_6.385041, whole genome shotgun sequence.
ACCESSION   DAOSCH010000018 DAOSCH010000000
VERSION     DAOSCH010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22824
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1627_length_22824_cov_6.385041"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..296)
                     /locus_tag="PLZ54_02715"
     CDS             complement(24..296)
                     /locus_tag="PLZ54_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ65882.1"
                     /translation="MNKAELISAVAEKSGLSKADAKKAIDAVVKSISEALIAGDKVSL
                     VGFGTFSVAERASRSGINPATKKPITIPAKKVAKFKAGAELSDAIN"
     gene            589..1377
                     /locus_tag="PLZ54_02720"
     CDS             589..1377
                     /locus_tag="PLZ54_02720"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOJ65883.1"
                     /translation="MSYRSSFFGSIPVVVKNLILINIILWLATLVLPRFFLRWGINVD
                     LVNILGMHYWGSKQFNPAQLITYMFLHGNFEHIFFNMFALFMFGTVLEQFWGPKRFML
                     YYMVTGIGAALIQQLFWSVEYQPVISALNEAINTGNKDALFSHEGVLSRFFRINNLAA
                     LDTTSLIELKHLFVNLPVTIGASGAIFGLLLAFGWLFPDAKLMLIFLPIPIRARIFVL
                     MYAVAELFLGVAGFTGDNVAHFAHLGGMLFGIFLILYWKKKGVH"
     gene            1454..2347
                     /locus_tag="PLZ54_02725"
     CDS             1454..2347
                     /locus_tag="PLZ54_02725"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOJ65884.1"
                     /translation="MNIFDNIKLSYRQGSHLIKLIYINLGVFLLYHAVLITFKLFNAS
                     GIFLLPFLAMPADGRQLLYHFWTPVTYMFMHEGFLHLFFNMLCLYWFGKIFLMYYSEK
                     QLTGLYLVGGWVAGLFYFLSFNIFPYFDAAVSQGLLLGASGSIMAIIVASAVASPNLE
                     MRILFLGGIKLKYIALVAILTSFFGITSNNAGGEIAHLGGALMGYLFVVSLRKGKDIT
                     KWLNGVLDNIVNLFSRKKLKIKKPKYSTSYRKMTDEEFNMEKSRRMQEIDRILDKIKT
                     SGYDSLTAEEKKRLFEQGKPR"
     gene            2414..3517
                     /locus_tag="PLZ54_02730"
     CDS             2414..3517
                     /locus_tag="PLZ54_02730"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOJ65885.1"
                     /translation="MILKKITKWGLISANIIVACALLFSVFAGFVSPAVFTFFAYFAL
                     VFPIIFFLNFAFFLLWIVLKKWYFLISLSLMLVSFPQLKTNFSFHLKKQPVSSIQPDF
                     TIISYNTHMMGLEKKHDEANKKINPVIQYVLSTDADIVCLQEFMVSTNEKYLTEEDAM
                     KAFSKYPYHHIYFKAHEKKSLLGIATFSKFPIINRQTIPYNSSANASIYSDINIHGKT
                     IRVINNHLESNQLTEKDKAMPYQLKDNFNTDLATNITVYFSKKLGAAYKLRAIQADEV
                     KKVISSSPHPVVVCGDFNDVPSSYVYTKIRGKLKDCFLEQGKGFGWTFNEKHYRFRID
                     YIFHDPSLVLLNFKKDKVPYSDHFPLKAGFQKL"
     gene            3734..4342
                     /gene="udk"
                     /locus_tag="PLZ54_02735"
     CDS             3734..4342
                     /gene="udk"
                     /locus_tag="PLZ54_02735"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007484842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOJ65886.1"
                     /translation="MLTIGIAGGTGSGKTTVVRKIIERLPAGEVVVLPQDSYYRDSSF
                     LPLEKRLEINFDHPDSIEFELMIKHLQDLKEGKPIEQPIYSYLTCTRAAETVTIYPRE
                     VIIVEGILVLCNPELRELMDLKVFVDADPDDRLIRVINRDIEERGRTVNKVIERYEHT
                     VKPMHELFIEPTKRFADLIIPQGGDNHIAIDILTKYIENNLK"
     gene            4364..5101
                     /locus_tag="PLZ54_02740"
     CDS             4364..5101
                     /locus_tag="PLZ54_02740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOJ65887.1"
                     /translation="MTNADLQKIMFFDIETVPQTSSFSELSNEMAQLWEEKFQKLRIR
                     MPEKYKEDTSASEAFDTSAGIYSEFGKIVCISVGFIYFQGETMYFRTKSFASDDEKEL
                     LKNFSDLISKFCSSKDNMLCGHNIKEFDIPYICRRLLINGLELPPVFQISGRKPWENN
                     FLDTLELWKFGDYKNYTPLKLLTAVFGIPTPKDDIDGSQVAQVYYKEKDLERIANYCT
                     KDVIATAQVFLRLNGFELISPQNIENL"
     gene            5112..6491
                     /locus_tag="PLZ54_02745"
     CDS             5112..6491
                     /locus_tag="PLZ54_02745"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOJ65888.1"
                     /translation="MQRNIIFIVYLLIIIFLTSCREGEKVKLSSENSFRDIQEIVKSD
                     TLRAGTMYGSTSYFLFREEWMGYDYEMVKNFADYLKVNLKVIPAATESELFQLLKEGK
                     IDIAAYNLPVTKALKKHFLYVFPQPQTYMVLVQNMGRKALSSVTDLIGKEVYVKKNTL
                     FYQRLNALNKEIGGGIKIVIAPDSVSNDELIEMVANNKIDYTIAYYDKALLHKSYYKR
                     LDCRLPVSFEQPNGWLIRKENTDLFKTLSTWGQLAGTQRTEDRLINLYWEKSPYFITR
                     KIKIPKGSISPYDEMFKKYAPTIGWDWRMLAAIAFHESKFDSAEISRAGAVGLMQLMP
                     QTAASFGLNHENWFNPEANLDAAVQYIKSLNLIFREIKDKDERIKFILASYNSGPSHV
                     LDAMALAKKYGKNPHIWFNHVETYLLKKTDPDYYRDPVVKYGFFRGAETVRYVQNVLD
                     TYEKYLSRK"
     gene            6687..9755
                     /locus_tag="PLZ54_02750"
     CDS             6687..9755
                     /locus_tag="PLZ54_02750"
                     /inference="COORDINATES: protein motif:HMM:NF020246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65889.1"
                     /translation="MKKLIVKPLIIAFQIILILLPTDAKAQYFGRNKPSYQTFKYQIK
                     QTPHFEIYHYLTNDTVIKYFAGWTEEWYRFHQLIFKDTFKTRNPLILYANHADFQQTN
                     AISSIISEGTGGVTESLKNRVILPFAPTLYQTDHVLGHELVHAFQYHKLLKPDSAKYY
                     NINNLPLWMVEGMAEYLSIGSVDANTSMWMRDALIQKKFPSINDLNNTSEYFPYRYGQ
                     ALWAFIGKTWGDSIILPLFEKTAQYGLDRAIDSLFHFNEKTLSGMWKTATENHYGKLM
                     KDTTYNVIGKQIISKKNSGSTNVSPSLSPDGKYIAFFSEKNVFTLDLYLADAQNGKII
                     KKLWSVNRNQEIDDFSFIESGGTWSPDSKQFAFVVYSKGRQKLAIVDVRKAKMKLLEI
                     KGLNSFSNPEWSPDGRYIVVTGLVNGIGDLYLYEVKSGKTEKLTHDLFSNIHASWSSD
                     GKKIVYSSEYLCGAGSKKFAFHINILDLETREIKTLDVFPKADNLNPRFSNNNQYIYF
                     VSDADGFRNLYKYDLNTDKVFRLTQYMTGISGITTFSPAISTDRKNDLIAYTYYFNGN
                     YEIYTASATEFNDEEVNGNQVNFDAATLPLLHHLSVNIVDSTLYNRPAVIALPVDSIN
                     NLPYRPKLKLDYISNSVGVGISTGPSYNTTDMAGSIYMIFSDMVGNHQIYSSLSLNGE
                     IYDFGGQVAYLNQGRKIKWGASLSHIPYRSGNMFWTIDTLKINNTDYIFDNLVLDYIR
                     MFEDNISLFSYYPLSQTRRFEGGISASWYSYRIDRYNNYYDALGYYVGGNRKKLPAPD
                     GISFQTANIAYVEDDSYFGMTSPLQGHRARYQIERYFGNINLYNALIDYRRYLFNKPV
                     GLAFRLYHNGLYGKSSQIELASPMYIGYPWLIRGYEQISVYGGANNVAGYSSLNVNNL
                     VGTRMALVNAEIRIPFSGPKVLAPIKSNIFLTDLNLFLDGGLAWNKGDQIKIKWEPSN
                     FNDRIPVWSTGISMRINLFGYLVIEPYYAIPFQNGGFRNKVFGINFVPGW"
     gene            10047..11000
                     /locus_tag="PLZ54_02755"
     CDS             10047..11000
                     /locus_tag="PLZ54_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HOJ65890.1"
                     /translation="MNNTIARINTCVNNALPKAFKTIIWLLKIILPISLGVSLLQYWG
                     IIATLSVYLTPLFSLVGLPGESAIVFITSIFMALYAPIAIITTLPLEMREITILALMC
                     LISHNLIVETAVQKKTGSSAFIMFSLRIISSFVTAFVLNKILPMNMGHEKIVQAGVSY
                     TDMGSMLLGWLKSSGWLILKISLIITGLMILQNILKEFNILDMLSKIFAPFMKIMGLP
                     KNCSFLWFVAQILGLTYGSAVMIESVENKEISPHDADLLNYHIAVNHSLLEDTLLFAA
                     IGVPVLWMIIPRFVLAVIILWGVHGIRALRRQFSHPEAEIV"
     gene            11056..11496
                     /locus_tag="PLZ54_02760"
     CDS             11056..11496
                     /locus_tag="PLZ54_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOJ65891.1"
                     /translation="MPEKTSRTKKLLIEVARKLFAERGKKNVTMNDIAETSGKGRRTL
                     YTYFKNKDEIYKAVIDSELIKIFQEIQSVSLEKIEPDIKLTKHIHKHLDAIKETVNRN
                     GSLKADFFRDIYEVERTRRKIDIQEIELIKSILREGIEKNSLNV"
     gene            11493..11657
                     /locus_tag="PLZ54_02765"
     CDS             11493..11657
                     /locus_tag="PLZ54_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65892.1"
                     /translation="MNVELSSIIILYALKGLEVPYIRENMGIEFEKNKNSIVDFVLAG
                     IKLPTSKEMI"
     gene            complement(11853..12581)
                     /locus_tag="PLZ54_02770"
                     /pseudo
     CDS             complement(11853..12581)
                     /locus_tag="PLZ54_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007485744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(13066..14766)
                     /gene="sulP"
                     /locus_tag="PLZ54_02775"
     CDS             complement(13066..14766)
                     /gene="sulP"
                     /locus_tag="PLZ54_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HOJ65893.1"
                     /translation="MHFQPKIFHTLKNYKKQQFYADLMAGVIVGIVALPLAIAFGIAS
                     GVTPEKGLFTAIIAGFIISLLGGSKFQIGGPTGAFIVIVYGIVQEFGISGLAIATMIA
                     GVMLICMGIFKLGSIIKFIPFPVVVGFTSGIALTIFTTQIKDLLGLQTGELPGDFIHK
                     WITYGKYIGTINYWALGVSLLSILIIVITPKFSKKIPGSLLAIVIVTLIVYFLREKFG
                     ITGIETIGDRFTINASLPHAETPTISFAVIQSLLPTAFTIAMLGAIESLLSATVADGV
                     IGDSHNSNTELIAQGIANVITPIFGGIPATGAIARTMTNINNGGRTPVAGIIHAIVLL
                     LILLFLGNLTRHIPMSCLAGVLVIVSYNMSEWRTFKALLRNSKSDVAVLVTTFLLTII
                     FDLTIAIEIGLLLAVVLFLRRISETTSISVFKNDINSAEYVEGNADTEKLTLPKGVEV
                     YEIEGPFFFGVASKFEETMKQIGDKPMIRIIRMRKVPFIDSTGLHNLTNLIKLSKKDK
                     IHILLSGVNPQVRESLEKSGIAEMLSEENICENITEAIEKAEKYVAAHEKKKSKKTEA
                     "
     gene            complement(15760..19476)
                     /gene="purL"
                     /locus_tag="PLZ54_02780"
                     /gene_synonym="purI"
     CDS             complement(15760..19476)
                     /gene="purL"
                     /locus_tag="PLZ54_02780"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOJ65894.1"
                     /translation="MIYFFQSSEKKIFVVQSAQLLETDISKLKWLFSGAELLDALWVE
                     GNFIGPRREMITPWSTNAVEITQNMGINGIVRIEEFREIPANIPLAEAEFDPMLERSY
                     NGLNQEIFNIDKVPEPIVYIDDIAAYNEKEGLALSKEEIDYLNEVSRKIGRQLTDSEV
                     FGFSQVNSEHCRHKIFNGKFIIDGEEKQLSLFQLIKKTSKENPNKLVSAYKDNVAFIK
                     GPDIEQFAPVKANVPDFFELKKISSVLSLKAETHNFPTTVEPFNGAATGTGGEIRDRL
                     AGGKGSLPLAGTAVYMTSYPRCHEENEKERQWENAVKPRKWLYQSPEQILIKASNGAS
                     DFGNKFGQPLICGSLLTFEHEENGKKYGYDKVIMLAGGVGFANERDALKEAAEPGEKV
                     VLMGGDNYRIGMGGGAVSSVETGQYDNAIELNAVQRANPEMQKRVANVIRALVEAPEN
                     PVVSIHDHGAGGHLNCFSELVENTGGKINIASLPIGDPTLSAKEIIGNESQERMGLLM
                     KEKDIEETKKIAQRERAPFYVAGETTGDMRLVFEQADGQKPLDLKLEYLFGKPPRTVI
                     TDKTLKDEFEPVKFDEEKLQEYLELVLQLESVACKDWLTNKVDRSITGKVARQQCVGE
                     IQLPLSDTGVVALDYRGTSGMATSIGHAPLAALIDPAAGSVLSVAEALTNIVWANLVN
                     GLDSVSLSANWMWPGKNEGEDARLYKAVEACSDFVCSLGINIPTGKDSLSMTQNYGKE
                     KVFAPGTVIISAAGEVADVKKTVSPVLVNDVDSAIYYVDFSFDSFKLGGSALAQVLNK
                     IGTETPTVKDAEYFKTAFDTIQELIEKNLILAGHDISAGGMIVALLEMCFPNTSGGLK
                     IQLDTLVEKSLITILFSENPSVLIQVQNAKTKVVEKILIDNGIGFARIAFPIPENRIE
                     ICKKKLSYCFSIAQLRDVWYRTSYLLDRKQSGKNCALQRYENFKNQPLTFSFPQTFNG
                     KLSQFGLSMHHAKTGAKAAIIREKGTNGDREMAFALYLAGFDVKDVHMTDLVSGRETL
                     EDVNMIVFCGGFSNSDVLGSAKGWAGGFLYNEKAKLALDNFYRRDDTLSLGICNGCQL
                     MIHLGLITPEHEQKPQMLHNDSHKFESGFVSVEIPENHSVMLETLTGSKLGVWIAHGE
                     GKFSLPKAKDDYHIAAQYVYPEYPGNPNGSDYNVAALCSSDGRHLAMMPHPERAIFPW
                     QWAYYPANRKNRDEISPWLEAFVNARKWIEKRKK"
     gene            complement(19743..21065)
                     /gene="mtaB"
                     /locus_tag="PLZ54_02785"
     CDS             complement(19743..21065)
                     /gene="mtaB"
                     /locus_tag="PLZ54_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOJ65895.1"
                     /translation="MIDTNIYKTKKAAYYTLGCKLNFAETSQLGKQLSAAGYRKVHAG
                     EEADVCIVNTCSVTDTADHKCRQAIKRLKRQHPNAIMVVTGCYAQLNPEEIAAIDGVN
                     LVLGAGEKFDVVNFIEQWNAENSVEIHKTPIQKVKEFHPSCSGTDRTRYFLKVQDGCD
                     YYCTYCTIPFARGRSRNASIRETVELATKAVAEGAKEIVLSGVNIGDFGKSTGENFFD
                     LVKALDALPEEVRFRISSIEPDLLTDELIEFIALSHHFMPHFHIPLQAGSNEALLLMK
                     RRYSTELFAHKVEVIKKWLPDAFIGVDVIVGTRGETEELFEKSKNFIASLDISQLHVF
                     TYSEREGTKMIQMEQNVPLAERKRRSDELHLLSEQKTQLFYESQIGKDAEVLWEGRHN
                     GSKMVGFTANYIRVERPYEKKKVNTIEKIKLGGWNDQHSALIALGYHD"
     gene            21496..21837
                     /locus_tag="PLZ54_02790"
     CDS             21496..21837
                     /locus_tag="PLZ54_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOJ65896.1"
                     /translation="MAYPKNKTYDFDIEELARFAKAMGHPARISILRYLASMDSCFFG
                     DICRELPIAKATVSQHLTELKNAGLIRGEIEPPKVRYCIDQENWEKASKAFRQFFANM
                     QPKDICCNPED"
     gene            21848..22267
                     /locus_tag="PLZ54_02795"
     CDS             21848..22267
                     /locus_tag="PLZ54_02795"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOJ65897.1"
                     /translation="MKILILCTGNSCRSQMAEAFLRSFDNRLTVCSAGTEPAEKVNEK
                     AIQVMREAGIDISRNRTKSVSQYLQETWDYVITVCADADRNCPVFSGKVKHRLHIGFD
                     DPAKVTGSEEFVLNEFRRIRDAIKEKFHQFYQENLQL"
     gene            22264..22620
                     /locus_tag="PLZ54_02800"
     CDS             22264..22620
                     /locus_tag="PLZ54_02800"
                     /inference="COORDINATES: protein motif:HMM:NF006749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HOJ65898.1"
                     /translation="MNVIYTIVKVLLSSVVLVAVSEISKRSTFVGSLLASIPLVSLLA
                     IVWLYIDTKDTAQIASLSTGIFWLVIPSLVFFIVLPLLLKRSLNFWLSLIISSVLTII
                     AYFIMLYLLKKSGIKL"
     gene            22632..>22824
                     /locus_tag="PLZ54_02805"
     CDS             22632..>22824
                     /locus_tag="PLZ54_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65899.1"
                     /translation="MEKNIKSVVKDTYTVIAQQSKEQNETSCCGVGGCCSTVDYAVFS
                     ENYRQLKGYHEEADLGLGCG"
BASE COUNT         6959 a         4427 c         4182 g         7256 t
ORIGIN      
        1 ttattaaaac tttaacaaac aaattaattg atagcatcgc ttaattctgc accggctttg
       61 aattttgcaa cctttttagc agggatagtg atgggttttt tggttgccgg attaataccg
      121 gaacgagaag cacgttcagc aaccgagaaa gtgccaaaac ctactaaact aactttatct
      181 cctgcaatta aagcttcaga gatactttta acaacagcgt caattgcttt ctttgcatca
      241 gctttgctta gcccagattt ttctgctacc gcactgatta attctgcttt gttcataaca
      301 taaaattttt ttaaagttaa acacactaaa aaatcctttt taaagctaca aatgtacatt
      361 attgaaaaca aatagcaagc atttgaataa aaaaatttct gtatttttta ctttttctta
      421 caaaatatgt tacaaaacat gttttagtta attttttcgc tacatttgca atttaaaaaa
      481 acgatttaat gaactatata ctccattaaa tgttttgaaa gttggtaaat ggttgtggaa
      541 agaagatttt gtctgaacaa ttttcttgaa taaaaaaata agtattgcat gagttacaga
      601 tcctcttttt ttggttctat acctgtggtg gttaagaatc tgattttaat aaacatcata
      661 ttgtggttgg caactttggt tttacctcgt ttttttctgc gttgggggat aaatgttgat
      721 ctggtcaaca ttctcggtat gcattactgg ggatcgaaac agtttaatcc tgctcaactg
      781 atcacttaca tgtttttgca cggaaatttt gagcatattt ttttcaatat gtttgccttg
      841 tttatgtttg gaacggtatt ggaacaattt tggggtccca aacgctttat gctgtattac
      901 atggttacag gtattggagc tgctcttata cagcaacttt tttggtctgt agaatatcag
      961 ccggtaattt cagcactcaa cgaagccata aacaccggga acaaagatgc cttgttttct
     1021 cacgaaggag tattgtcccg tttttttaga atcaacaatt tggcagcttt agacaccact
     1081 tcattaattg aactgaagca tctttttgtg aatctacccg taaccatagg tgcttcaggt
     1141 gcaatttttg gtttattact ggcatttgga tggcttttcc ccgatgctaa acttatgctt
     1201 atatttctcc cgattcctat tagagcccga atatttgtat tgatgtatgc agtagccgaa
     1261 ttatttttag gggtggctgg ttttacgggt gataatgttg ctcattttgc ccatttgggc
     1321 ggtatgctct tcggaatttt tttgattttg tattggaaga aaaaaggtgt acattgatgt
     1381 aagtattctt aaaaaattta tataaagaat ataatttatt gatttacatt gttttatttt
     1441 caaatttttt gctatgaata tttttgacaa tattaaatta tcctatcgtc agggaagtca
     1501 tctgattaaa ctcatatata tcaacctcgg tgtgtttttg ctgtaccatg ctgttttaat
     1561 cactttcaaa ctattcaatg ccagtggtat ttttcttctt ccatttttgg ctatgccggc
     1621 agatggaagg caattgcttt atcatttctg gacaccggtt acttacatgt tcatgcacga
     1681 aggtttcctt catctgtttt tcaacatgtt gtgcctttac tggtttggaa aaatattttt
     1741 gatgtattat tccgaaaaac aacttaccgg tttatatctg gtgggaggat gggtagccgg
     1801 attgttttat tttctgtcat ttaatatttt cccctatttc gatgcagctg tttcacaagg
     1861 tttacttttg ggtgcttccg gttctataat ggccattatt gtagcttcag ccgtcgcttc
     1921 accgaatctg gaaatgcgga tactgtttct cggcggcata aaattgaaat acattgcatt
     1981 ggtggcgatc cttacaagtt ttttcggaat cacgtccaac aatgccggag gtgaaattgc
     2041 tcatttggga ggagctttaa tgggttacct ttttgtggta tctttacgta aaggaaagga
     2101 tattaccaaa tggctaaatg gagtgctgga taacattgtg aatctttttt cgagaaaaaa
     2161 actcaaaata aaaaaaccca aatattcaac ttcttaccga aaaatgaccg atgaggagtt
     2221 caacatggaa aagtcccgac gaatgcaaga aatagacaga atactcgaca aaattaaaac
     2281 atcgggttac gacagcttga cggcagaaga aaaaaaaaga ctttttgaac agggaaaacc
     2341 aagataaatt ttttgtcgat ccaatcggaa atcgtttctt tccccaaaat caattattga
     2401 aacagacggt aaaatgatcc taaagaaaat tacaaaatgg ggtttaatta gcgcaaatat
     2461 tattgtagca tgcgctttgc ttttcagcgt ttttgcaggt tttgtaagtc ctgcggtatt
     2521 tacttttttt gcttattttg cacttgtttt tcccattatt ttttttctta attttgcatt
     2581 tttcctttta tggattgtgc taaagaaatg gtattttctc atttccctga gcttaatgct
     2641 tgtttctttc cctcaattaa aaacaaattt ttcttttcat ttaaaaaaac aaccggtttc
     2701 ttcaattcag ccagacttta ccattattag ttacaacact cacatgatgg gactcgaaaa
     2761 aaaacacgat gaagcaaaca aaaaaataaa tccggttatt caatatgtac tttcaaccga
     2821 tgccgacatt gtatgcttac aggaatttat ggtcagcaca aacgaaaaat atcttaccga
     2881 agaagatgcc atgaaagctt ttagcaaata tccgtatcat catatctatt tcaaagcaca
     2941 tgaaaaaaaa agcttgttgg gaattgccac attctctaaa tttcccatta tcaatcgaca
     3001 aaccataccc tacaattctt cagccaatgc ttccatttat agtgatataa acattcacgg
     3061 aaaaaccata agggtaatca ataatcatct tgaatccaat cagttgacgg aaaaggacaa
     3121 agccatgccc tatcagttga aggataattt caacaccgat ttggcaacga acataactgt
     3181 ttatttttca aaaaagcttg gagccgccta caaactcaga gcaattcagg ccgatgaagt
     3241 taaaaaggtt atttcttctt ctccccatcc tgttgttgtt tgtggcgatt tcaatgatgt
     3301 cccttcttcg tatgtatata caaaaattcg cggaaaactc aaagactgtt tcttggagca
     3361 gggaaaagga tttggatgga ctttcaacga aaagcactat cgatttcgaa tcgattatat
     3421 tttccatgac ccgtctctgg ttttattgaa tttcaaaaaa gataaagttc cctattccga
     3481 tcatttccct ttgaaagcag gttttcaaaa attatagaat gttggtctct tacccaagaa
     3541 ttttgtcagt agaaaagaca atggattctt taagaataaa aagctaataa aaattttttc
     3601 atagcttaaa ttatttgttt ttaggcatat agaatttatc ccaataagaa atattaaaaa
     3661 aacattgaaa attaaatgaa agaaagcgta taaattctta ttttagtgct ctgaaataaa
     3721 acaaaatatc aatatgttga ccataggaat tgccggagga acaggttcgg gaaaaaccac
     3781 cgtcgttcgc aaaattatag aaagattacc tgccggagaa gtagtggttt taccacaaga
     3841 ttcgtattat cgcgacagca gttttcttcc tctcgaaaaa aggttggaga ttaattttga
     3901 tcatcccgat tccattgaat ttgaactgat gataaaacat ttgcaagacc tcaaagaagg
     3961 aaaacccatc gaacagccca tttattctta tctcacgtgc acgcgagcag ccgaaaccgt
     4021 tacaatttat ccacgcgaag taatcattgt ggaaggaatt ttggtgctgt gcaatccgga
     4081 attacgtgaa ctaatggatc tgaaggtttt tgtggatgcc gatcccgatg accgtttgat
     4141 tcgggttatc aaccgcgata ttgaagaacg cggtcgtacg gtgaacaagg ttatcgaacg
     4201 ttacgagcat accgttaaac ccatgcatga actttttatt gaacccacca aacgttttgc
     4261 cgacctcatt attccacagg gtggagataa tcatattgct attgatattc ttaccaaata
     4321 tatcgaaaac aacctgaaat aagaaaaaaa accgcaaaac aatatgacaa acgccgattt
     4381 gcaaaaaata atgttttttg atattgaaac tgttcctcaa acttcaagtt tttctgagct
     4441 ttctaatgaa atggcccaac tttgggaaga gaaatttcaa aaacttagaa tcaggatgcc
     4501 cgaaaaatac aaggaggaca catcggcaag cgaagctttc gatacttcag ccggaatcta
     4561 ttccgaattt ggcaaaattg tctgtatttc cgtaggtttt atctattttc agggagaaac
     4621 catgtatttt agaaccaaat cgtttgcttc cgacgatgaa aaggaacttt taaaaaattt
     4681 ttccgacctg atttccaaat tttgctcatc aaaagacaat atgctttgcg gacacaacat
     4741 caaggaattt gacataccct atatttgcag acgcctgctt atcaatggat tggaacttcc
     4801 tcccgtattt caaatatcgg ggagaaagcc gtgggaaaac aattttctcg ataccctgga
     4861 gctttggaaa ttcggcgatt acaaaaatta cacacccttg aaattgttaa ccgctgtttt
     4921 cggaattcca actccaaaag atgatattga tggaagtcaa gtcgctcagg tttattacaa
     4981 ggaaaaagat cttgaacgca ttgccaatta ttgtaccaaa gacgtcattg ctaccgcaca
     5041 ggtatttttg cgcttaaacg gatttgaatt gatttcaccc caaaatatag aaaatctata
     5101 acatttcgat aatgcaacga aatataatat tcatcgttta tctcctgatt attatattct
     5161 taacatcctg tcgagaaggg gaaaaggtaa aattatcttc cgaaaattct ttcagagata
     5221 ttcaagaaat tgtcaaatcc gatacattaa gagccggaac catgtatggc tcaacatcct
     5281 atttcctttt cagagaagaa tggatgggtt atgactacga aatggtgaaa aattttgccg
     5341 actacctgaa agttaattta aaggtgattc cggcagctac cgagtccgaa ctatttcaac
     5401 tgcttaaaga aggaaaaata gatattgctg catataacct tcctgtaacc aaagctttaa
     5461 aaaagcactt tctctacgtt tttcctcaac ctcagacgta tatggtattg gttcaaaata
     5521 tggggcggaa agcgctttca agtgtaacgg atttgatagg aaaagaagtt tatgtaaaaa
     5581 agaatacact tttttatcaa agactgaatg ccttaaacaa agaaattggc ggtggaataa
     5641 aaatagttat cgctcccgat tcggttagta atgatgaatt gatagaaatg gtggccaaca
     5701 ataaaattga ttataccatt gcttactatg acaaagcttt gttgcacaaa tcatactata
     5761 agcgtctcga ttgccgttta cccgtgagtt ttgagcaacc caacggttgg ttaattagaa
     5821 aagaaaacac tgatttgttc aaaacccttt caacatgggg tcaactggct ggaacacaaa
     5881 gaaccgaaga tcgacttatc aacttatact gggaaaaaag tccgtatttt attacccgta
     5941 aaataaaaat acccaaaggg tccatttctc cctatgacga aatgtttaaa aaatatgccc
     6001 caactatagg atgggattgg cgaatgctag cagcaatagc ttttcatgaa tccaagtttg
     6061 attcggcaga aatatcaagg gcaggagcgg tagggttgat gcagctcatg cctcaaacag
     6121 cagcctcttt tggtcttaat catgaaaact ggttcaatcc cgaagcaaat ttggatgcag
     6181 cagtacaata tattaaaagt ttaaatttga ttttcagaga aataaaagat aaagatgaac
     6241 gaataaaatt tattttggcc agctacaatt caggaccttc gcatgttctc gacgcaatgg
     6301 ctctggccaa aaaatatggt aaaaatcccc atatctggtt caaccatgtt gaaacttatc
     6361 tcctaaaaaa gactgatccc gattactatc gcgatccggt tgtaaaatat ggcttttttc
     6421 gcggtgcgga aaccgttcgg tatgtacaaa atgtattgga cacttatgag aaatacttaa
     6481 gcagaaaata agacattttt tcatagttgt tcctttttat tccattgttt tacaagaatt
     6541 ttctacaatt tttcattctg gcatttattt tgtttaaaac tttttgttct ttttacccga
     6601 gacaaaagcg acatgatttc agatttgttt tattgattgt ttgtttttca tagtcaattg
     6661 aattgagaac cacagaaagt tgaattatga aaaaattaat tgttaaaccg ctcataattg
     6721 cgttccagat aattttaata cttttaccga ctgacgcaaa agcccagtat tttggacgaa
     6781 ataaaccaag ctatcagact tttaagtatc aaataaagca gactccgcat tttgaaatct
     6841 atcattacct tactaacgat acggtaatca aatatttcgc aggttggact gaagaatggt
     6901 atcggtttca tcaattgata ttcaaagata cttttaaaac cagaaatcct ctgattttgt
     6961 atgccaatca tgccgatttt caacaaacaa atgctatcag cagcattatc agcgaaggaa
     7021 cgggcggcgt aaccgaaagt ctgaaaaatc gcgttattct gccttttgct cctaccttat
     7081 accaaaccga ccatgtgctg ggacacgaat tggttcacgc ttttcaatac cacaaactgt
     7141 taaagccaga ttcggccaag tattacaaca taaacaatct tcccctgtgg atggtagaag
     7201 gaatggcaga atatctttcc attgggagcg ttgatgccaa cacttccatg tggatgagag
     7261 atgcactgat tcagaaaaaa tttccatcca ttaacgattt gaacaatact tccgaatatt
     7321 ttccttatcg ctatggacag gctttatggg cattcatagg taaaacatgg ggcgattcga
     7381 taattctgcc gttatttgaa aaaaccgccc aatacggact ggatagagcg attgacagcc
     7441 ttttccactt taacgaaaaa accctttccg gcatgtggaa aacagctacg gagaatcatt
     7501 acggaaaatt aatgaaagat acgacgtata atgttatcgg gaagcaaata atttccaaaa
     7561 aaaattcagg ttctaccaat gtttcgccat ctttaagtcc ggatggtaaa tatatcgcat
     7621 tcttctccga aaaaaatgtt tttacgcttg atttatattt ggctgatgcg caaaatggaa
     7681 aaataatcaa gaaattatgg agtgtcaacc gaaatcagga aatagatgat ttcagtttta
     7741 tagaatcggg aggaacttgg tcgcccgaca gcaaacaatt tgcttttgtt gtttacagca
     7801 aaggccgtca aaaactggct attgtagatg tacgcaaagc caaaatgaaa cttttggaaa
     7861 taaaaggatt aaattcattt tcaaatcccg aatggtcgcc tgacggcaga tatattgtcg
     7921 ttacgggttt ggtaaacggg attggggatt tatatcttta cgaagtaaaa tcaggaaaaa
     7981 ccgaaaaact gactcacgat ttgttttcca atattcatgc ttcatggtct tcggatggca
     8041 aaaaaatagt atattcttcc gaatacttat gtggtgccgg aagtaaaaaa tttgcatttc
     8101 atataaacat tctcgacttg gaaacccgag aaataaaaac cttagatgtt ttcccaaaag
     8161 ccgataacct gaatcctcgc ttttcaaaca ataaccaata tatttatttt gtttccgatg
     8221 ctgatggatt cagaaatctg tataaatatg atttgaacac cgataaagtt tttcgtttaa
     8281 cgcaatacat gacaggaatt agcggaataa ctacattctc gccagcaatc agtacagaca
     8341 gaaaaaatga cttgattgcc tatacttact atttcaacgg caattacgaa atttatacag
     8401 cttccgcaac cgaatttaac gacgaagaag taaacggaaa tcaagtaaat ttcgatgccg
     8461 caactttgcc tttgttgcat catttatccg tcaatattgt tgattctacg ctttacaatc
     8521 gcccggcagt gattgcactt cctgtcgatt caatcaataa tctgccctat cgacccaaat
     8581 taaaactcga ttatatttca aattcggtcg gggttggaat ttcaacaggt ccaagttaca
     8641 acaccaccga tatggccggt agcatataca tgattttcag tgatatggtt ggcaaccatc
     8701 aaatttattc ttccttgtcg ctgaatggag aaatttatga tttcggcggt caggtagctt
     8761 atctgaatca gggaagaaaa atcaaatggg gagcatcctt atcgcatatt ccctacagaa
     8821 gcggcaacat gttttggaca attgatacgc tgaagataaa taataccgac tatattttcg
     8881 acaatctcgt tttggattac atccgcatgt ttgaagacaa tatttcgctg ttcagttatt
     8941 atccactctc tcaaacacgt agatttgaag gaggaatttc tgcatcgtgg tactcatacc
     9001 gcatcgacag atacaacaat tattatgatg cgttgggcta ctatgtggga ggaaatcgta
     9061 aaaagcttcc tgctccggat ggcataagtt ttcaaacagc aaatattgct tatgtggaag
     9121 atgattccta cttcggcatg acctcgccct tgcagggaca tagagcaaga taccaaatag
     9181 aaagatattt tggaaacatc aatttataca atgctttgat tgattacaga agatatttgt
     9241 ttaacaaacc tgttgggctc gcatttcgac tttatcacaa cggtttgtac ggtaaatctt
     9301 ctcagattga actggcttca ccaatgtata ttggataccc gtggttaatc agaggatacg
     9361 aacaaatatc ggtttatgga ggagcaaaca atgttgcagg ctattcttct ttgaatgtca
     9421 ataaccttgt gggaacacgc atggcacttg tcaacgctga aattcgtatt cctttcagtg
     9481 gaccgaaagt actggctccc attaaatcaa atatttttct taccgatctg aatctgtttt
     9541 tggatggtgg attggcatgg aacaaaggag atcagataaa aatcaaatgg gaaccatcca
     9601 atttcaatga tcgtattcct gtgtggagta caggaatttc catgagaatc aatttgtttg
     9661 gctatttggt aatagaacct tactacgcca taccattcca aaatggaggt ttcagaaata
     9721 aagttttcgg aatcaatttc gtacccggat ggtaatttta aaactaattt aatttcttga
     9781 atatcttttt gttaatacag aatcaaaacg aaaagtctca tattttcagt agtcgaaaaa
     9841 gcaagacgat gcatcaataa gttaaatgtt gttaaatatc aatttttttc tccgaatatg
     9901 ttgtacaaca taaaaaatcg atgtacattt gcacatgttt ttcatggtat tagatttaag
     9961 gttaattaaa aagattgggt tgtcgtgaga caacctttct tgtttttata acttccattc
    10021 tttgcaattt tttttacttt acttcgatga ataacaccat tgcccgaata aatacatgcg
    10081 taaataatgc actcccaaaa gctttcaaaa ccattatatg gcttttaaaa attatcttac
    10141 caatcagttt gggagtcagt ttactgcagt attggggaat tattgccacc ttatccgttt
    10201 accttactcc attgttttca cttgtagggc ttcccggtga atcagccata gttttcatta
    10261 ccagtatttt tatggcatta tacgctccca tagccattat cacaacattg ccactggaaa
    10321 tgcgtgaaat caccatcttg gctctcatgt gtctgatttc tcataacttg attgtagaaa
    10381 ctgccgttca aaagaaaacc ggttcttctg catttatcat gttctcgctg cgtattattt
    10441 cttcttttgt tacagctttt gttctgaaca aaatacttcc catgaatatg ggacacgaaa
    10501 aaattgtaca agcaggcgta agctataccg atatgggaag tatgcttttg gggtggctga
    10561 aaagttccgg ttggctgatc ttaaagattt ctctcattat taccggattg atgattcttc
    10621 aaaacatttt gaaagaattc aacatacttg acatgctgtc gaaaatattt gctccattca
    10681 tgaaaataat gggattaccg aaaaactgtt cttttttatg gtttgtagcc caaattttag
    10741 gattaactta tggttcggct gtcatgatag aaagtgtcga gaacaaagaa atcagccctc
    10801 atgatgccga tttgttgaat tatcatattg cagtaaacca ttccttgctg gaagatacct
    10861 tactttttgc tgctattggg gttcctgtat tgtggatgat aattccccgt ttcgttttgg
    10921 cagttatcat tttatggggt gtgcatggca tcagagcatt gcgccgccaa ttttctcatc
    10981 ctgaagcaga aattgtttga caatcaataa aaaaccgagc tgagaaaaat tttccctgtt
    11041 tcaaacatat tttttatgcc cgaaaaaaca tccagaacaa aaaaattatt gatagaagtt
    11101 gcacgaaaat tattcgctga aagagggaaa aaaaatgtta ccatgaatga cattgccgaa
    11161 acttcaggga aaggaagaag aactttatat acttatttca aaaacaaaga tgaaatatat
    11221 aaagccgtaa ttgacagcga actgatcaaa atttttcagg aaattcaatc tgtttcatta
    11281 gaaaaaatcg aaccggacat taaacttacc aaacacatac acaaacattt agatgccata
    11341 aaagaaaccg taaatcgaaa tggttcgctc aaagccgatt tttttcgcga tatttatgaa
    11401 gtagaacgca cccggcgtaa aattgacatt caagaaattg aactcattaa atccatcctg
    11461 agagaaggaa ttgaaaaaaa ctctttaaac gtatgaacgt cgaattgtcc tctatcatta
    11521 tcttatatgc tttgaaaggg ctggaggttc cctacatcag agagaatatg ggtatcgaat
    11581 tcgaaaaaaa caaaaacagc atcgttgact ttgtactggc cggaattaaa ctaccgactt
    11641 caaaggagat gatttaattt ggattaaata agaaagtacg ctattaagat accatactta
    11701 cccacaaaat agaacactcc tccgctgata acaaaagatt atatggaaaa cactgttttt
    11761 tgccggatgc tttgtggtaa aaaatcacta ctgcctgatg atttttagtg ttagtttttt
    11821 attcattaaa tttgaaagtt gtattcattt caaaatgttc ccaaccacga cttcaattct
    11881 gtcaacattt ccaaatggta tttgaaagat tctctgattc cccagccatg accgccgtcg
    11941 gaataaatat gcatggttgc aggaatgttt tgctgttgca atgccaaata gtattttaca
    12001 gaattcacag gttttaccac atcgtcgtca tccgacaata cgataaaagc tcgcggtgtg
    12061 ttggcattga cctgcaattc gttactgtat aatttttcca tttcaaatgt tggatttttt
    12121 cctagcagat gttcgcgcga accggcatgt gtttcttgtt tgttcatggt gataaccgga
    12181 taaaacaaaa tttgaaaatc aggccgtaat tctgtaggcg catgagttgc cactgtagca
    12241 gccaaatgac ctccggcaga agaacccatt atcccaatac aatcgggatg aatattccaa
    12301 gttttggcat tcgttttagt tagtcggatg gcttcataag catcggaaat tggcacttcc
    12361 aaatgtccgt taggcatgcg gtattttaaa acaataaccg ctattccctg attgttgaaa
    12421 aacgaagccc aatcgtaacc ttcatgattc atggctaaat gctgataacc acctccgggg
    12481 caaataacta cagcctttgt tggttgcgaa gctttgggaa gataaacggt aatgcagggt
    12541 ttgaagttcg atttggcatc atcgtaaccc aatgtttcca ttccgttggt atttggtaat
    12601 ccgttttgcc agagatcaat agtaaaaggt gatgttggag tggaaatttc tttatcctgt
    12661 gcaccaacaa aaggacaaac caccaacaaa agcagcaaaa ataagggctg aaaagaataa
    12721 aatgttttca tcagaatata atttattttt taaaggtctg atggaaccca catgtctgaa
    12781 tattgtattc attcgtgttt gtgtatgcaa tacatgtggg tttcattgaa atctgttcca
    12841 atttcagaaa ttttttcctt aattttgtcc ggtagcatat ttttctgttt ttgtaattaa
    12901 catctaatat actcattttt aatgatgtat tacataaaat acgctgcttt ttattcattt
    12961 tttctcttct tttcccttta ttttcattac tttctgaact gcgaaacttt agtaaatttt
    13021 ttatctgtta taactatatt tttatttttt gaatgcaaag tctgcttacg cttctgtttt
    13081 ttttgatttc tttttttcat gggcagccac gtatttttca gccttttcta ttgcttctgt
    13141 gatattttca caaatgtttt cttcagacag catttcagca atacccgatt tttcaagtga
    13201 ctcacgcact tgaggattta cacctgaaag aaggatatga attttatctt tttttgaaag
    13261 ttttatcagg ttagtaaggt tatgcaagcc tgtagaatca ataaatggca cctttcgcat
    13321 acgtattatt cttatcattg gtttatcccc aatttgcttc atggtctctt cgaatttact
    13381 tgcaactcca aaaaagaacg gtccctcaat ttcatacact tccacacctt ttggtaaagt
    13441 tagtttttct gtatcagcat tcccttctac atattcggct gaattgatgt catttttgaa
    13501 aaccgatatg gaagtagttt cagaaatacg acgcaaaaaa agtactacag ccaataacaa
    13561 cccgatttcg atggctatgg tcagatcgaa aattattgta agtaaaaaag tggtaaccaa
    13621 tacagctaca tctgattttg agtttcggag caatgcttta aaagtgcgcc attcgctcat
    13681 gttgtacgaa acaataacga gtactcctgc cagacaagac atgggtatat gacgggtgag
    13741 attgcctaaa aaaagtaata tgagcaacaa cacaatggca tgtatgattc ctgccaccgg
    13801 agttcgtcca ccgttattaa tatttgtcat tgttcttgct atggctccgg tagccggtat
    13861 tccgccgaaa atgggagtta taacatttgc tattccctgt gctatcaatt ctgtgtttga
    13921 gttatgacta tcgccgataa ctccatcagc aactgtagca gaaagcaatg attcgatggc
    13981 accaagcatg gcaattgtaa atgcagtggg caataacgat tgtattactg caaaacttat
    14041 tgttggagtt tcagcatgag gcagtgatgc attaatagta aaacgatctc caatagtttc
    14101 gataccggtt atgccaaatt tctccctgag aaaataaaca atcagtgtaa caatgacgat
    14161 tgcgagcagt gatccgggaa ttttttttga aaattttggt gttatcacaa taatgagtat
    14221 tgacagcaaa ctcaccccga gcgcccagta gttaatagta ccaatatatt ttccgtaggt
    14281 aatccatttg tgaataaaat cgcccggaag ttcaccggtt tgaagtccga gcaaatcttt
    14341 gatttgagtg gtgaaaatgg tgagcgctat accactggta aaacccacta ctacaggaaa
    14401 tggtatgaat tttataatac tgccaagctt gaaaataccc atacatataa gcatcacacc
    14461 ggcaatcatg gtagcaatgg caagtccgga tatgccaaat tcctgcacaa tgccatacac
    14521 tataacaata aatgcaccgg tagggccgcc aatctgaaat ttgctgcctc ctagaagcga
    14581 aataataaaa cctgcaatta tggcagtaaa caaacccttt tcgggtgtaa ctcccgatgc
    14641 tataccaaaa gcaatagcaa ggggcagggc cacgattcct actattacac ctgccatgag
    14701 gtcagcgtaa aactgttgct ttttgtaatt cttcagcgta tgaaaaattt taggctgaaa
    14761 atgcataaca tgaaattttt tttttaattt gaagtgcaaa gttactgcat ttcaacgaac
    14821 tttatccttc taaatatgtt ttttagcata aaaacaaact gaatcgttgc ctttaatgac
    14881 tttaatcttc agaacaattg agtcaattat ccttttaatt tcaaatttaa atgtaatttt
    14941 gtcattcaaa ttttatcagc aaaaatttat gtatagattc acttagtaca agagaaaaaa
    15001 ttgtacaaat atttatatta tttattttca gatatttaat aacggggctt tgccccaaac
    15061 cccacttact tttttgtctt gaaacaaata agtaagcaaa aaaatttact tagttgaagc
    15121 agctacgctc aacataaatc aaataaattt gattttgttt tcacttaaac gctactttca
    15181 atagtgtgtc caattcactc gaaaaactta ggctcgaaaa gctgaaatcg cccaaactca
    15241 ttccgacgaa gtacgtcgga actcaaacta aaaagattta taaaggcttt tctcactttt
    15301 tttcggctca ctggacaagg tcgacccgaa gagatcggct tataagtatt gttttacaga
    15361 tatacaaatg tctcaatttt tctcctgtac tgaaaaaagg aataaaaaaa ttggtcattc
    15421 gataaacttg tcattggttt tttgctttct gtactccact cgagtatttc cgtaaaataa
    15481 cgtgagctcg atataacgat tacatgtttt attcatataa aaatttgata attaataagg
    15541 taataaggtt tatttttgtt tttgtgatta tcgttactaa ggttttattt aaaaatatgg
    15601 ttcgtttctt gaatataaag actttattta gagataacta accttagcaa catgtgaata
    15661 tcatcttttc atcaacataa taagcttatt ttatgaactc acgttaaaat aataattttt
    15721 tcgatggggg tcaaattttt ataccgaacg agttcaaatt tatttctttc ttttttcaat
    15781 ccattttctg gcattgacaa aagcttccag ccagggcgaa atttcgtctc tgttttttct
    15841 gttggcggga taataagccc attgccaggg aaagattgcc cgttccgggt gtggcatcat
    15901 ggctaaatgt cgtccatccg aagaacaaag agcggccacg ttataatctg aaccattcgg
    15961 attaccggga tattcgggat aaacatattg tgctgcaatg tggtaatcat cttttgcttt
    16021 tggcaatgaa aatttgcctt ctccgtgtgc gatccaaact cccagcttgc ttccggtcaa
    16081 tgtttccaac atgactgaat gattttctgg aatttctaca cttacaaaac ccgactcaaa
    16141 tttatgggaa tcgttgtgca gcatttgtgg cttttgttca tgttcaggcg ttatcagtcc
    16201 caaatgtatc atcagctggc aaccattgca aataccgaga ctcaatgtat cgtctcttcg
    16261 ataaaaatta tccagcgcca atttggcttt ctcgttatac agaaatccgc ctgcccaacc
    16321 ttttgcagag cccaacacat cggaattgga aaatcctccg caaaatacaa tcatattgac
    16381 gtcttccaat gtttcgcgcc cggaaaccaa atcggtcatg tgcacatctt tgacatcaaa
    16441 tccggccaga tatagcgcaa aagccatctc acgatcgcca ttcgtgcctt tttcccgaat
    16501 tatggcagct tttgcaccgg tttttgcatg atgcatggac aagccgaact gcgaaagttt
    16561 tccgttgaaa gtttgaggaa aagaaaaagt caatggctga tttttaaaat tttcataccg
    16621 ttgcaaagca cagtttttgc cactttgttt gcggtcgagc aagtaggaag tacgatacca
    16681 aacatctcgc aattgggcaa tagaaaagca gtaggataat tttttcttgc aaatttctat
    16741 tcggttttcc ggtatgggaa atgctatgcg tgcaaaaccg attccgttgt ctataagaat
    16801 tttttcaact actttggttt tggcattttg gacctgaatc aaaacgcttg gattttccga
    16861 aaaaagaatg gttatcaacg atttttcaac cagcgtatcc aactgaattt tcaagcctcc
    16921 gcttgtattg gggaaacaca tttccaataa agcaactatc attccgccgg cagaaatatc
    16981 gtgtccggcc aaaattaaat ttttttcgat caattcctgt atggtatcga atgccgtttt
    17041 aaaatactct gcatctttga cggtgggcgt ttcggtgcca attttgttta aaacctgtgc
    17101 cagtgccgag ccacccaatt taaagctgtc gaaagaaaaa tcgacgtaat aaatagccga
    17161 atcgacgtcg tttaccaata caggcgaaac ggttttttta acatcggcaa cttcgccggc
    17221 agccgaaata atgactgttc cgggtgcaaa aactttttct tttccgtaat tttgagtcat
    17281 ggaaaggcta tccttccctg tcgggatatt gatacccaac gaacagacaa aatcactgca
    17341 agcttctaca gctttataaa gtcgtgcatc ttctccctcg tttttccccg gccacatcca
    17401 gtttgcactc aacgacacac tgtccaatcc attcaccaaa tttgcccaaa cgatattggt
    17461 caaggcttct gccacagaca acaccgaacc tgctgccgga tcaatcagcg cagccaaagg
    17521 cgcatgtccg atggaagttg ccatgcccga agtgcctcga taatccaatg ctacaactcc
    17581 cgtatcgctt aaaggaagtt gaatttctcc aacgcattgt tgacgggcaa ctttgccggt
    17641 gatggaacga tccactttgt tggtgagcca atctttgcag gctaccgatt ccaattgtaa
    17701 aacaagttcc agatattcct gtaatttttc ttcatcaaat tttaccggtt caaattcatc
    17761 tttcaaagtt ttgtcggtga taacggttcg cggtggttta ccgaaaagat attccaattt
    17821 taaatccagt ggtttttgcc cgtctgcttg ctcaaaaacc agacgcatat cgccggttgt
    17881 ttcgccggcc acataaaacg gagcacgttc gcgctgggct attttttttg tttcttcaat
    17941 atctttttct ttcatcaata aacccattcg ttcctgactt tcgttgccga taatttcttt
    18001 tgcacttagt gtcggatcgc ctatgggaag cgacgcaata ttgatttttc ctccggtatt
    18061 ttcaaccaat tcggaaaagc agttgagatg accgccggcg ccatgatcgt gaattgaaac
    18121 aacaggattt tccggcgctt ccaccagagc acgaataaca ttggcgactc ttttctgcat
    18181 ttcgggattg gcacgctgaa cagcattcaa ttctatagca ttgtcatatt gtccggtttc
    18241 aacagatgaa acggctcctc cacccattcc gatgcgataa ttgtctccac ccatcaaaac
    18301 aactttttct cccggttcgg ccgcttcttt cagtgcatcc cgctcattgg caaaacctac
    18361 acctccggcc agcatgatta ctttgtcgta accgtatttt ttcccgtttt cttcgtgttc
    18421 aaaagtcagc agagagccac aaataagcgg ttgaccaaat ttatttccga aatcggaagc
    18481 tccgtttgaa gctttaatta atatttgttc cggagactga tacaaccatt ttctgggttt
    18541 taccgcgttt tcccactgac gctccttttc attttcttcg tggcatctgg ggtaacttgt
    18601 catatatact gccgtacccg ccaatggcaa acttcctttt cctcctgcca gtcggtcgcg
    18661 aatttctccg cctgttccgg tagcagcccc attaaatggt tcaacggtgg tgggaaaatt
    18721 atgtgtttcg gcttttagcg aaagaacaga tgaaattttt ttcaattcaa aaaaatccgg
    18781 aacatttgct ttgacaggtg caaactgctc gatgtcgggc cctttgatga aagctacgtt
    18841 atccttgtag gccgaaacca gtttgttggg attttctttt gaagttttct ttattagctg
    18901 aaatagagat agttgctttt cttctccatc aataataaat tttccgttga aaatcttgtg
    18961 gcgacaatgc tcggaattta cctgcgagaa tccaaaaact tcactgtccg taagttgtct
    19021 gccaattttc cgactcactt cattcaaata atcaatttct tccttgctca atgccaaacc
    19081 ttccttttcg ttgtaggctg caatatcgtc gatgtaaacg attggttcag gaactttgtc
    19141 gatgttgaaa atttcctgat tcaaaccatt gtagctccgt tcgagcatgg gatcaaactc
    19201 cgcttcggca agtggaatat tagccggaat ctccctgaac tcttctatac gaacaatgcc
    19261 gttgattccc atattttgag ttatttcaac ggcatttgta ctccaagggg ttatcatttc
    19321 acggcgtggc ccaataaaat ttccttctac ccaaagggca tccagcagct cagctccgga
    19381 aaacagccat tttaacttgg agatatcggt ttccagcaat tgagcagact gaacgacaaa
    19441 gatttttttt tctgaagatt gaaagaagta aatcatattt tttgaggatt agttgttttc
    19501 cttattaagc gtaaaatatt taattgtcgg taattttcag agaatatttc taaaaattca
    19561 aatcacgatg tctaacaaag taagccgttt ttcggcaaaa aaactttttc atcagaatat
    19621 aatttgtttt ttaaaggtct gatggaaccc acatgtctga atattatatt cattcgtgtt
    19681 tgtgtatgca atacatgtgg gtttcataat tgcgcaaacg gaattgatat gataagctta
    19741 aattaatcat ggtatcctaa ggctattaag gccgaatgct gatcattcca gccgcccagc
    19801 ttgatttttt cgatggtatt gactttcttt ttttcataag gtcgttccac ccgaatataa
    19861 ttggccgtga aacctaccat ctttgagcca ttatgccggc cttcccataa gacttcggcg
    19921 tccttaccga tctgactttc gtaaaataac tgagtttttt gttcggacag gagatgaagt
    19981 tcatcactgc gccttttcct ttctgccaat ggaacatttt gctccatttg aatcattttt
    20041 gttccttctc tttcggaata ggtaaatacg tgaagttgag agatatccag cgaggcgata
    20101 aaatttttgc ttttttcaaa aagttcttca gtttctccac gtgttcctac aattacgtca
    20161 actccgataa aggcatcagg caaccatttt ttaataactt caactttgtg agcaaaaagt
    20221 tcggtcgaat atcgtctttt catcaatagc agggcttcat tgctgcccgc ctgcaaagga
    20281 atatgaaaat gaggcatgaa atgatgactg agagcaataa attctatcaa ttcatcggtc
    20341 aacaaatcgg gctcaatgga tgaaattctg aaacgaactt cttcaggaag cgcatccaat
    20401 gcttttacca gatcaaagaa attttctcct gtcgattttc cgaaatcacc aatgttgaca
    20461 ccggaaagca caatttcctt tgcaccttca gcaacggctt ttgttgccaa ttccaccgtt
    20521 tcccgaatgg atgcatttct gctcctgcct cgcgcaaaag gaatagtgca ataagtacaa
    20581 taataatcgc atccatcctg tacttttaaa aaatatcgtg tacgatcggt tccggagcag
    20641 gaagggtgaa attctttcac tttttgaata ggtgttttat gaatttcaac cgaattttcg
    20701 gcattccatt gctcaataaa attcaccaca tcaaattttt caccggcacc cagcaccaaa
    20761 tttacgccat caattgcagc aatctcttcc ggattgagtt gagcgtaaca ccctgttacc
    20821 accatgatgg cattcggatg ctgacgtttg agtcgtttga tggcttgcct gcatttgtgg
    20881 tcggccgtat cggtaaccga acaggtattg acaatacata catctgcttc ctcgcccgca
    20941 tgtacttttc ggtaacctgc agccgacaat tgtttgccca actgagatgt ttcggcaaag
    21001 ttcagtttgc aacccaatgt ataataagcc gcttttttgg ttttatatat atttgtgtca
    21061 atcatttgtt gcttactcga attcttctgc aaaattacaa aaaaacagcc aatttctttt
    21121 tatcatatct ttgttgaatc ctctgaagga aaatattttc tgatgagaat caataaaaag
    21181 ttggttgtaa tacataaata aaaaataatc gacaaaaagg aaagaagatg tggccatgat
    21241 taaattgtgt tggtttgatt tcaactcatt actatgtaaa aaacttcgat ttgttgcttt
    21301 tgcagtctgg gccaagttaa ataaacgcta caagtgttgc ttctcgggca aaaactttga
    21361 tgtgaaaaat ggacaatctc aaacattcag tttttaactt cccattaatt gacttgcaaa
    21421 aaaatatcac tgatttttta ttttgaatat tattcgtaat tttgcgaaca acaaattcaa
    21481 atcatccata caccgatggc ttaccctaaa aataaaacct acgacttcga catcgaagag
    21541 ttggcgcgtt ttgccaaagc aatgggacat cctgcccgca tcagcattct tcggtacttg
    21601 gcttcgatgg acagttgttt cttcggcgat atttgccggg aacttcccat tgcaaaggct
    21661 accgtatcgc aacacttgac ggaattgaag aacgccggat taatcagagg cgaaatcgaa
    21721 ccgcccaaag tgagatactg catcgatcag gagaattggg aaaaagccag taaagctttt
    21781 cggcaattct ttgcaaacat gcaacccaaa gacatttgtt gcaatcccga agattaattt
    21841 tacgtatatg aaaatactta tcctttgcac cggtaacagt tgccgaagcc aaatggcaga
    21901 agcatttctg cgttcgtttg ataatcgttt gacggtttgc tctgccggta ccgaaccggc
    21961 ggagaaagtg aacgagaagg ccattcaggt aatgcgtgaa gccggtatcg atattagccg
    22021 gaacagaacg aaatcggtca gtcagtatct gcaggaaacg tgggattacg tgattaccgt
    22081 ttgcgccgat gccgaccgca attgtccggt attttcggga aaagtaaaac accggctgca
    22141 tattggtttt gatgatccgg ccaaagttac aggttctgaa gaatttgtct taaacgagtt
    22201 cagaagaata agggacgcaa taaaagaaaa attccaccaa ttctatcaag aaaatttaca
    22261 gctatgaatg tcatttatac catcgttaaa gtactgcttt cgtcggttgt attggtagcc
    22321 gtttccgaaa tatccaaacg gagcactttt gtgggcagcc tgttggcttc cataccgctg
    22381 gtttcgctgc tggccatcgt ttggctgtac atcgacacca aagacacggc acaaattgcc
    22441 tcgttgtcca ccggcatttt ctggctcgtg attccttcgc ttgtcttttt tattgtgctg
    22501 cccctgttgc tcaaaaggag tctcaacttt tggctgtcgc tgattatctc ttcggtgctg
    22561 acgatcatcg cttatttcat catgctctat ttactcaaaa aatcaggaat aaaactctaa
    22621 cattcaattc tatggaaaaa aatatcaaat ccgttgttaa ggacacttac acggtgattg
    22681 ctcagcaatc gaaagaacag aacgaaactt cctgctgcgg cgtgggcgga tgttgttcta
    22741 cggtcgatta cgccgtattc agtgaaaatt accgccaact gaaaggttat cacgaagaag
    22801 ccgatttggg attgggatgc ggca
//
LOCUS       DAOSCH010000019        22082 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1682_length_22082_cov_6.615290, whole genome shotgun sequence.
ACCESSION   DAOSCH010000019 DAOSCH010000000
VERSION     DAOSCH010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 22082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22082
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1682_length_22082_cov_6.615290"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(489..956)
                     /locus_tag="PLZ54_02810"
     CDS             complement(489..956)
                     /locus_tag="PLZ54_02810"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOJ65900.1"
                     /translation="MIQRIQTFYLFLVLILSSVTLFSPVAVLFNKESATLYEISYKGL
                     CLLDESSKVPVSSVWALTAISAIVPVVTLIIIFLYNKRLIQIRLSIFNMIIMLGYYVI
                     LFIYLFYAAKTLHADWSLKIVSAFPLVNIILTLLAMRAIAKDEALIKSLNRLR"
     gene            complement(1023..2009)
                     /locus_tag="PLZ54_02815"
     CDS             complement(1023..2009)
                     /locus_tag="PLZ54_02815"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HOJ65901.1"
                     /translation="MADNSLKKILKHYFKKIKTFFLSKDIFIFLLFFVFSAGLWFVNA
                     LGKERERIISIPLNYTGIPQHIAIVNNPPKSLSVKIKDEGMHLLQYRQKRLKPLDIYF
                     SQIFYEKGKILITPDQISNYLLRYLQPTTLVLETKPDSIVVEYEKLSQKLLPVECKIK
                     YSLAQQYMIVDKILLQPSKITVFGTAQQINSLKTLKTEPLLLSNLNDTVNTICRLQPI
                     HGVRFTTEAIQVFIPIEKFTEKKVRLPIQVLNVPNYITIRTFPTQVEATFNIALSRFS
                     SFDPAQIQLAVDYHQIKESASDKLQVFVIDHPSYVQNIRLSPQQVEFLVEKF"
     gene            complement(2019..2348)
                     /gene="yajC"
                     /locus_tag="PLZ54_02820"
     CDS             complement(2019..2348)
                     /gene="yajC"
                     /locus_tag="PLZ54_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOJ65902.1"
                     /translation="MNLTAILLQATPAANGTGGGYTGILMMILIFVVFYFFLIRPQTK
                     RQKEIKKQRESMKVGDHVVTSGGIYGKIKEVKDNTMIIEIAENVRIKVDKNSVFLSPE
                     DIQSQAK"
     gene            complement(2425..3375)
                     /gene="nusB"
                     /locus_tag="PLZ54_02825"
     CDS             complement(2425..3375)
                     /gene="nusB"
                     /locus_tag="PLZ54_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOJ65903.1"
                     /translation="MINRILIRIKIVQVLYSFYKNDEKSFTVAEKELFHSIEKTYDLY
                     FHLLQLAVEITRFANLRIDARKNKLRPSPEDLNPNMRFVENSFVEQLSTNKQFTKYLT
                     ERGLSWSDYPEVIKRLYEQITASDFYAEYMNAPFVDYEVDKNLWKKIYKKIILQSEEL
                     DSSLEEQNIYWINDIELVVSFIIKTIKKFQLSAGAQQELLPMFRDEEDVSFAAKLLKN
                     AISNCDEYRALIDKHTKNWELDRIAFMDIIIMQAALAELMEFPTIPVNVTLNEFIEIA
                     KIYSTEKSGTFINGILDNIVGQLKKENKLIKVVMFNENKK"
     gene            3642..4004
                     /locus_tag="PLZ54_02830"
     CDS             3642..4004
                     /locus_tag="PLZ54_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOJ65904.1"
                     /translation="MEDVTRKEDLNKQPNEIVYSRAIKAGKRIYYLDVKKNKNDNFFL
                     AITESKKKVLGSDEDAQVSFEKHKIFLYRQDFEKFLEGLQDVMKFIRLQQPEEPYAES
                     HGKSSETTDEQDDFFLTK"
     gene            complement(4199..4381)
                     /locus_tag="PLZ54_02835"
                     /pseudo
     CDS             complement(4199..4381)
                     /locus_tag="PLZ54_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769314.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-phosphate reductase"
     gene            complement(4462..5061)
                     /locus_tag="PLZ54_02840"
     CDS             complement(4462..5061)
                     /locus_tag="PLZ54_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65905.1"
                     /translation="MKKYIYLAFLCSFTLISCDENKEPAVTLKNEIKVKVFSTDTWDP
                     TTNKMDTVVGATVNLISDSGTSSVITDNKGIATFSNVTEDDYIIVAKKDNLCNLINTS
                     LEGDMTIGNLIIGVYTSEKDIEQSAFYANARVGGNKLADANGDGLINNLDKVRGQSSE
                     FEYQYKDLNGDGTIDAKDLSNGNLIQIDHQVEETIFIGK"
     gene            complement(5181..6440)
                     /locus_tag="PLZ54_02845"
     CDS             complement(5181..6440)
                     /locus_tag="PLZ54_02845"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOJ65906.1"
                     /translation="MNATNHSYDWKEAALNAARTLQLVDDKAISETLLALADEIAIGS
                     EKILKANAEDLAAMEKAYPMYDRLLLNEQRLQNIAADIRNVAGLPSPLGKTLMETVRP
                     NGMKISKITVPFGVIGIVYEARPNVTFDVFSLCLKSGNSCVLKGGSDAYHSNAAIVSL
                     IREVLNRFGLNPDIITLLPSGHEATADLLNATGYVDLVIPRGGKGLIDFVRTHSKIPV
                     IETGAGICHTYFDEFGNTEKGSKIILNAKTRRVSVCNALDCLIIHEKRLSDLPALCKK
                     LAEKNVVIYADAKAYTFLVGKYPVHLLQKATQESFGTEFLDYKMAIKTVESLQEAIQH
                     INCYGSKHSECIVSENEANIALFDAMIDAACIYTNVSTAFTDGAQFGLGAEIGISTQK
                     MHARGPMALNELCTYKWIIKGNGQVRE"
     gene            complement(6510..7265)
                     /locus_tag="PLZ54_02850"
     CDS             complement(6510..7265)
                     /locus_tag="PLZ54_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ65907.1"
                     /translation="MPEFENVLENREFQTLPSVSVDCVIFGYEGNLLKVLVRREYVPF
                     EGNILIEWKLPGNHVRRNEQIDATASRILKEQTGLENIFMKQFMVFSDPDRLKRRERD
                     YEWVKPRLQDDRVLTVGFYSLVNIHDVDNSKLLEEAKWENAYEIRELMFDHNDIFNEA
                     LKKLRYDLLHEPLIFELLPEKFTLTQMQKVYEAIFDTTYDKRNFRRKVNKMPYLIQTD
                     ELQSGVSHKPARYYTFDREIYENTRSERFDFRM"
     gene            7502..9814
                     /locus_tag="PLZ54_02855"
     CDS             7502..9814
                     /locus_tag="PLZ54_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOJ65908.1"
                     /translation="MKNKRLNLFYLLLFFIPIFSAISAQESVSDRVEELLSKMTIEEK
                     VGQMAQITLDAIGNGATVFESKIPFAIDSVRLHKAIVDYHVGSVINTTNNYALSALQW
                     NSIIDQIQKTATTKTTLRIPVIYGIDAIHGVTYTSGATLFPQQIAIAATFNPGNAYNM
                     AKVTAYETRASGIAWNFSPVLDLGADPRFSRQYEGFGEDPYLASVMGRQAIRGYEGIT
                     DSIGHAEKVAACAKHFLGYSVPFSGKDRTPAYIPDHILREYHLPSFKAAIDEGVESVM
                     INSGIINGVSVHANYNIITKLLKEELGFQGVVVSDWMDIINLEKRDKIAKNNKEAIAL
                     AINAGIDMSMVPYDYEQFCKDLTALVKEGKVKESRIDDAVRRILKLKFKLGLFENPVT
                     NLSNYPDFGSDKHEKLAYKAAADAITLLKNKNNILPLNKNIKILVTGPNANSMRSLNG
                     GWSYSWQGEKTERYAAQYHTILEAVQQKIGEKNVLYEPGVSYVETGQYFEEKENGLQK
                     AVAAARKVDAIVLCLGENSYAEKPGDLNDLNLSELQIKLAKSLSAVNKPIILVLNEGR
                     PRCFSAIESKMNAIVQTYLPGNYGGDALADILFGDVNPSGKLPYTYPAYPNSIVNYYH
                     KFSEEQKPSEGAYKYESDYNPQYEFGFGLSYTTFAYSNLKISSSVLYPNELIEVSVDV
                     TNTGTREGKETVLLFTSDVIASITPDMRRLRRFEKINLKPNETKTVKFTLSANDLAFV
                     NTDNQWTIEPGEFEIKIGNLKKTINYQSTN"
     gene            9930..12842
                     /locus_tag="PLZ54_02860"
     CDS             9930..12842
                     /locus_tag="PLZ54_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ65909.1"
                     /translation="MPLALSAQKTTITGVVVDSNNEPVIGATVLIKGTTTGTVTNYEG
                     KFTIEASSNDVLVVSYIGMVPQEIKVGNSKNINVVLKEDIKILSEVVVVGYGTQKKKD
                     LTTAVSTVSAEDFNVRPIVSAGQAIQGKAAGVQVIQPNGAPGGETVIRVRGTTSFNGN
                     SDPLYVVDGVPVDNINFLSPNDIENINILKDASSAAIYGSRAANGVVIITTKQGKSGE
                     SKIAFNAQLTAKNVTNKIESLNAADYKDLMEEVRPGSSSQMSSDDITNWYDEVYRTGL
                     QQSYQLSFSNSTEKTSYFLSAGYLDEKGIFPASFFKRYSFRANMTNEIRKWLSIGSNI
                     SYSDNSNNGITTGAGSNRGGVVLALINLPTSLPVINPETGYYNRSFYGQNITNPVEAM
                     ENGKDDITRENRLIASGNAMITFMKGMNFKSTFTLDRRHGLQTGFSPVIHVTGIDEYG
                     SAWDNRNMNTVLTWDNIFNYQKSFDKNNIELMMGSSWTDSKYTNSWINGSHYRNNKIK
                     TLNVANKISWNNTGTGGSTWGIMSYFGRVSYNYNSKYLITANFRADGSSRLHPDHRWG
                     YFPSFSAAWRISSEEFMQGLNWLDDLKIRGGWGQTGNQLGLGDYAYLQRYSVTRQQWW
                     ITGQENILPIITASNLRTTDLTWETTTQSNVGIDFTAFKSRLNIIMDYYYKKTTDMLL
                     NITLPSGANVTSIQRNGGLMRNTGFELSVNSINITNKNFKWTSDLEFSLNRNKLVKLD
                     LTKIYYDAATSDAFHGIQIVRNQEGRSLGGFYGYISDGVDSETGELIYRDINGDNKIT
                     ASDRTFIGDPNPDFIYGFTNTFTWKGFSLSIFLQGTYGNDIFNASRIETEGMYDLKNQ
                     STRVLDRWRRPGMITTIPKANFNLQPSSYFVEDGSYLRLKDLTLSYNVTAPFLKKSGI
                     SRLQPYITASNLLTFTKYLGMDPEVNEWGNSNAVQGIDWGTYPQAKSFVFGFNIEF"
     gene            12856..14406
                     /locus_tag="PLZ54_02865"
     CDS             12856..14406
                     /locus_tag="PLZ54_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOJ65910.1"
                     /translation="MKIIKYILISAVVFVAASCSLDYNPIDTYSDITEGIDTTSAASQ
                     VILKDKAAVLNQRQVLYQRLKDGQEHWYLDMLLLAESHSDNAYAGSPNAETTPFEINS
                     IEGSNPNLKRDWNGHMDNIAQANRLIQGIDELKDPSLTEAEAKQIRAEGLIFRSLVYF
                     DMVRIWGRVPLITTVGKDITADNIEEVYPAYFPQQSEEIDVYRQIEEDLLEAVRYAPN
                     NDPSNKTLLTKSVARALLAKVYAEKPLRDYNKVIAYADSVAADGFELVSDFSDLWGVQ
                     LQNPSAPPGENNFAIDAKKRNTSEAILEIQYPVGSGNWVTWMFGRSLDNWNFWFDWAK
                     WITPSRDLINAFQAAGDIKRYNESVVWYSCSWNIYYPSNNYPFMYKTRSAYNSIIKIR
                     YADILLLKAEALIQTGDLSGAASIINDKIRKRAGLAPLPASVTNDKNALLSAYLNERR
                     LELAFEGQRWFDLVRLEKVEEVLNTLPSRDSGRPALRYPYDKYSYKLPIPQDIIDQNP
                     NLVQNPGY"
     gene            14426..15853
                     /locus_tag="PLZ54_02870"
     CDS             14426..15853
                     /locus_tag="PLZ54_02870"
                     /inference="COORDINATES: protein motif:HMM:NF027723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5016 domain-containing protein"
                     /protein_id="HOJ65911.1"
                     /translation="MKNIIKLTIFGLLGLFATSCQQEELRVHYPASKPVFDSISVPET
                     NIVYGDSITLYVGVSDPLTPLSTLEIKVVVNDELIASETIRTKGNQATYHQRYGIPFV
                     ARMPDNAEVEIHLTSINVEGTKTDSIYVNTIAKRPTITDLYLVVPLKSPVKMKLTDAQ
                     NYIYTADNLSLENTVSFRLATKLKYGISIDWTGLVFGKVGNGIGLIDINGSPITLSDA
                     TLIGYKKITVDLFNFTVKGEGDKLQPATAMDISTFGTVSLSSTDYLNVTTKEDWKTKK
                     MYLGKGTEMTISGIPTTNLANNLAPDFFEVTGSNTAKFLGETGIYTVYYLPRLNFVFV
                     EQPDAVYPDAMWLCGVGFGPARTPHIKTSSWNWNTPLEYQFCRKVSDGVYQATFYALH
                     QIDDTAAEPWRLKFSVKFFHQRMWGGEEDARTYTLNVNGLLFAPTESDQGNFAGTDAF
                     KDKPGIYRLTIDVNNKTANFQKISD"
     gene            15911..17359
                     /locus_tag="PLZ54_02875"
     CDS             15911..17359
                     /locus_tag="PLZ54_02875"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOJ65912.1"
                     /translation="MNKKSIFVFLFLLWVFIFSTGCKHSGDEPVPTPTPQDSVGDVSV
                     WLTTGNESCLLKKMAPINFSRDFADFTIVIDTTTTYQYIDGFGAALTGSSAHLIHQMS
                     AQNRKKLLDDLFDPEKGIGINYLRITIGSSDFSIGTYSYCDSPDINKFDIPEIDRNEL
                     LPVLKEILEINPSMKILASPWSAPAWMKSNNSMYGGSLKGEEVYNDFAEYFVRYVQVF
                     EQEGIHIDALTIQNEPMHETGGYPTMKMLWQEQNKIIRDYLGPKFIANGIQTKIIIWD
                     HNFDMSYYPINILNDPVTRQYVNGTGWHGYKGNASAIDQVTALYPDKETYFTEISGGG
                     WNTETRIKNMFYYMKDFLMATVNRGSKNFLMWNLALNPQHGPTTTTWGGCQDCRGVVT
                     INENETYIRNEEYYLLGHFAKVVRLGAVRVNNSCYNLPSGMTISSFMNADGSKVVVIL
                     NRSGSSRKYNVRCGTRKFYYQQVDESVVSFKF"
     gene            17407..18780
                     /locus_tag="PLZ54_02880"
     CDS             17407..18780
                     /locus_tag="PLZ54_02880"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65913.1"
                     /translation="MKIRALFFLMAFAIVTNCISAGKVTVWSSLPDTKALLVKKSMKL
                     NSKPVEIKNKVEIFPDQTCQSVEGVGAALTHSSAYVFHHNLTAQRRDSLFKVLFTPAG
                     IGINYTRLCIGASDFAFNLFSYSETEDFSLSNFNIQEDEKDVIPMLKEILAINPNLQI
                     LASPWSPPGWMKTSGSMVGGKLRPDCYDVYAEYLIKYIEAYRQHGITIHALTVQNEPE
                     YGTAAYPCMDMTAKEQQIFIRYFLGPKMQKKGIQTKIILFDHNCDNPDYPISILNDPD
                     TKKFVDGSGFHLYKGEISALCKVHEAHPDKNIYFTEQSGGGWAPDFDQNVRWYVGELF
                     AGAMNCWSKNVLLWNLALDENDGPKNFGCQNCWGVVRVTSDGRIEKNAEFYAIGHYGK
                     VVQPGAVRLVSNQDILKGVAFRNPDNSFAYLAVYYGTTPQTIGIQCGNSSFVYTFKPG
                     EVVSFLW"
     gene            complement(19105..19830)
                     /locus_tag="PLZ54_02885"
     CDS             complement(19105..19830)
                     /locus_tag="PLZ54_02885"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOJ65914.1"
                     /translation="MLTENVFYIPQTVSTNVLCWKMLKERDIPEGFVVVADYQTAGKG
                     QAGNSWESARGKNLLFSIVLYPHHIPIEEQFLISQAVSVGIKNVLDTVSDDITIKWPN
                     DMYWKDSKLAGILIETSLFRGKISKAVIGVGLNVNQTEFFSDAPHPISLKQIAGKSFN
                     RKKLLHRIVASILEVYGQWESEKIRSVYAQSLYRKNGYHFYQAGTEIFRAKISRIYPD
                     GALELETEKGTSAKYYFKEIKFL"
     gene            complement(19841..21250)
                     /gene="dacB"
                     /locus_tag="PLZ54_02890"
     CDS             complement(19841..21250)
                     /gene="dacB"
                     /locus_tag="PLZ54_02890"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOJ65915.1"
                     /translation="MKKIFVNVFVSFFVLNLVAQTPVDNFIHHPLLKNANVSLLIRET
                     GKGKVVGRYNANKSIIPASTLKLVTTASALELLGPNFRFQTKLQISGNVTKEHVLQGN
                     LIIKGAGDPTLGSEFLGEKNFLDQWVLAIKQAGISKIEGKIIADASIYNQEGVSPFWI
                     WEDIGNYYAPSIFGISYLDNTFRLVLRSGPIGTTPAIIRTLPDLPDLVIENYLKSSKI
                     TFDSAYFCGMPNSNLRRLYGEIPANRAEFVVKGDIPNPVLLLAKQFSDKLIQSGITVN
                     NPPVAEFSTPFDGQVVYTHFSPPLRDIIKQTNERSNNHYAECLFRYLGLGEGVPVTGR
                     GGVKTIRNLWKLKGLPIDQLFMCDGSGLSPMNAVSAQFITELLDYMYSKSAFSKDFFN
                     SLPIAGKTGTLVSFLAGTPLEGKVFAKSGSIEKVKCYAGYVKNEGKTYTFVIMVNTAQ
                     GTSRAVTKKIEELLLSVFK"
     gene            21701..>22082
                     /locus_tag="PLZ54_02895"
     CDS             21701..>22082
                     /locus_tag="PLZ54_02895"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HOJ65916.1"
                     /translation="MKNFTFLLLFSVFGGELLCHASGEQANIISLKNDSLLFSSDQIE
                     LDAFDSIPEKNIKEVVVYGISSGRLTFPLSLVSKTSLLNSSFTTPADALQRETGIFMS
                     RDGIWATSVNIRGLSEQRLLFLVDE"
BASE COUNT         6875 a         4038 c         4264 g         6905 t
ORIGIN      
        1 tttcagaaaa aatcaagtgg agttaaaaat ttatctaagt acatgaataa atttaaaatt
       61 atggatattt aactaaaaac taaaatttaa aatccgtgtt gcaatgaaaa gaagatcata
      121 tttaatatga cttttttccg cttactgatg gtggcagagg aagtatgaaa aattagaccc
      181 aattgaaaat ttgtaaaata actttcttta atttactttc taatgtgaac atttttacgg
      241 cattcgaatg ataaacctgt tgcataattt tttggtttaa ttttgaataa attaatcaag
      301 tttttcattc gtaggttctt ttttaaaatt atgttgccgg ttaacggagc gtgctttgga
      361 gtatgtacaa cgcaatttcg taacccaccg atactgctaa attcacactt ctatatagaa
      421 cttagctctc aactttttaa tttacacact tcgtgaaata gtgtccaatt tcctgtcttt
      481 acacaatttc atctgagcct attaagtgat ttaattaaag cttcatcttt ggctatagcc
      541 ctcatggcca acagggtaag aatgatgttt accaatggaa atgccgaaac aatttttaaa
      601 ctccagtcgg catgaagtgt tttggctgca taaaataaat aaataaacag tattacataa
      661 taacccagca tgatgatcat attgaaaata gaaaggcgta tttgaatcaa tcgtttgttg
      721 taaagaaaga tgataatcag ggttaccacc ggaactattg cactaatggc agttaatgcc
      781 caaaccgaag aaacaggtac ttttgaactt tcatccaata aacacaaacc cttatagctg
      841 atttcgtaaa gtgtggcaga ttccttgtta aagagcactg caacaggcga aaaaagagta
      901 acggaagaaa gaattagcac caaaaacaaa taaaaagttt gaatgcgttg aatcataaat
      961 ttcgatttaa gtggttaaga aaagtaaatt tacgatgata aaaacatgaa aataaaaaaa
     1021 tattaaaatt tttcaaccaa aaactctact tgctgaggag aaagtcgaat attttggaca
     1081 tatgatggat gatcaatgac aaaaacctgt aatttgtcgg aagctgactc tttaatttga
     1141 tgataatcta cagccaattg aatttgggca ggatcaaagg aactgaacct gcttaaggcg
     1201 atattaaaag tagcttccac ttgagtaggg aaagtacgta ttgttatata atttggtaca
     1261 ttcaatactt ggataggcaa acggactttt ttttcggtaa acttttctat cgggataaaa
     1321 acctgaatgg cttccgttgt aaaacgaacc ccatgaatgg gttgaagtcg acaaattgta
     1381 tttacagtat cattgagatt tgaaagaaga agaggctctg tttttaacgt tttcaagctg
     1441 ttgatctgct gcgcagtccc aaaaacggtt attttgcttg gttgaagaag aattttatcg
     1501 actatcatgt attgttgtgc caaagaatat ttgattttgc attctacagg caacaacttc
     1561 tgactaagct tttcgtattc cacaacaata gagtcgggtt tggtttcgag caccagcgta
     1621 gtaggctgaa gatagcgtaa aagatagtta ctgatttgat cgggagtgat caaaattttt
     1681 cctttctcgt aaaaaatttg agaaaaataa atgtcaaggg gcttcaatct cttttgccga
     1741 tattgcaaaa ggtgcatccc ttcatcctta atttttactg acaaagattt gggtggattg
     1801 ttaacgatag ctatatgttg gggaatgccg gtatagttca atggaattga aataattctt
     1861 tcacgttctt ttcccaaagc atttacaaac caaagtcctg ccgaaaacac aaaaaaaagc
     1921 aaaaagataa aaatatcttt tgacagaaag aatgttttta tctttttgaa gtaatgtttg
     1981 agtatttttt ttaagctgtt gtcagccata ataccctctt attttgcttg cgattgaata
     2041 tcttcaggac ttaggaacac ggaattttta tctactttga tacgaacatt ttcagctatt
     2101 tcgattatca ttgtattgtc ctttacttcc ttgatttttc cgtaaatgcc acctgaagta
     2161 accacgtgat cacctacttt catcgattcg cgctgtttct tgatttcttt ttggcgttta
     2221 gtctgtgggc gaatcaggaa gaagtaaaac accacaaaaa ttaaaatcat cattaaaatt
     2281 ccggtatatc ctccgcctgt tccgttggct gcaggagttg cctgcaataa aattgctgtt
     2341 aaattcatag atctataatt tatttgttat ttaatatttg atatggtaaa gtgtttcttt
     2401 tttaatgagt tgcaaatata agtattattt tttgttttca ttaaacatta ccacttttat
     2461 tagtttattt tcttttttta attgtccaac aatattatcc aaaataccat tgataaaagt
     2521 accacttttt tcggtactgt aaatttttgc aatttcaata aattcattta gagtaacatt
     2581 tacaggaata gtaggaaatt ccatcaattc agctaatgca gcctgcataa taatgatatc
     2641 cataaaggca atcctgtcca attcccagtt tttggtatgt ttgtcgatca gcgccctgta
     2701 ctcatcacaa ttgctgatgg cattttttaa aagtttggca gcaaacgata catcttcttc
     2761 gtctctaaac atgggtaaaa gttcctgctg agcacctgcc gaaagttgaa actttttaat
     2821 ggttttaatg ataaaactga caaccaattc tatgtcgttt atccagtaaa tattttgttc
     2881 ttccagcgag ctgtccaatt cttcactttg aagaataatt tttttataaa tttttttcca
     2941 gagatttttg tcaacttcat aatcaacaaa gggagcattc atgtactcgg cataaaaatc
     3001 ggatgccgta atctgctcgt aaagcctttt tattacttcc ggataatccg accaggatag
     3061 tccgcgttca gtcaaatatt tggtaaactg cttgtttgta gataattgtt ctacaaaact
     3121 attttcaaca aaacgcatgt tgggattcaa atcttcgggt gaaggacgca atttgttctt
     3181 tcgggcatct atgcgtaaat tggcaaaccg tgtgatttct acagccaact gtagcagatg
     3241 aaaataaaga tcgtaggttt tttcaatact gtgaaaaagt tctttttcag ctacagtgaa
     3301 cgatttttcg tcgtttttgt aaaaagaata taaaacttga acaatcttaa tccgaattaa
     3361 aatacgatta atcataaaat cagtggacgc ttataaataa cgcacaaaga taagtatttt
     3421 ttttgttccc tcaacttttg ctctgcagca aaaaatcgat caactatttc agaaaaaaac
     3481 caaaatatgt ccctatttag agaaaatgag aaatttattt gaataattaa aaaaaaatac
     3541 tgaaatttgg agaaaattga cttcaactat aaaattttat tataaatttt tatagttatt
     3601 tgaaatacag tgttttaatt taaaaagaga ggttgaaaat tatggaagac gtaacaagaa
     3661 aagaagattt gaataaacaa cccaacgaaa ttgtttattc acgagccata aaagctggaa
     3721 agagaatcta ctaccttgat gttaagaaaa acaaaaacga caactttttt ttggccatta
     3781 ccgaaagcaa aaaaaaggtt cttggcagtg atgaggatgc tcaggtatca ttcgagaaac
     3841 ataaaatttt tctttaccgt caggattttg aaaaatttct tgagggactg caagatgtaa
     3901 tgaaatttat tcgtcttcag caacctgaag aaccttatgc tgaatcacat ggaaagtctt
     3961 ctgaaaccac agacgaacag gatgattttt ttttgacaaa ataaattttt aggaaaaaac
     4021 aaagagaaaa ttcaatagga ggatagcttt ttcgttgtcc tttttactcg attaattttt
     4081 acaagtaaga gaaggtacat ttatgaaaat aaccatgtga ttaaactttt tccgttatat
     4141 gttattttgc aatcaaataa attaggaagt taatgtaatt tcactcttcc ggatattttt
     4201 attctcttac ttgcccattc cctttgatga tccatttgta ggtacaaagt tcgtttaacg
     4261 ccattggacc gcgagcgtgc attttctgcg tactgatgcc tatttcagca cccaatccga
     4321 actgagcgcc atcggtaaaa gcggtagaaa cattggtata aatgcaggct gcatcgatca
     4381 ttgcatcgaa taaaaaagag agattgcttt acgcgtttaa atcttagtgc ctttttgtca
     4441 gaaggctgcc gaagattact tttattttcc tataaaaatt gtttcttcaa cttgatgatc
     4501 gatttggatc agattgccgt tagataaatc tttcgcatca attgttccat ctccattcaa
     4561 atctttatat tgatattcaa attccgaaga ttgacctcgc actttatcga gattgttaat
     4621 caatccatct ccattggcat cggcaagttt atttcctccc actcttgcat ttgcgtaaaa
     4681 tgctgattgt tcaatgtctt tttcagaagt ataaacacca ataatcaaat ttccaatagt
     4741 catgtctcct tccaaagaag tattgatcag attgcacaaa ttatctttct tagccacaat
     4801 tatatagtca tcttctgtaa cattagaaaa agttgcaatt cctttattat ctgtaattac
     4861 agaagatgtt ccagagtccg atattaaatt gacagttgcg ccgacaaccg tgtccatttt
     4921 gtttgtggta ggatcccatg tgtctgtgct gaagactttt actttaattt cgtttttcaa
     4981 ggttactgcc ggttctttgt tttcatcgca gcttatcaag gtaaatgagc aaaggaatgc
     5041 aagataaatg tactttttca tttttttaga ttgttattgt aaattaactt ttaaatattt
     5101 tgacaaagat aattataaga acatgaaata aatacatttc aggaacataa attttctttt
     5161 tgattaattt ttttaaaaat ttattctctt acttgcccat tccctttgat gatccatttg
     5221 taggtacaaa gttcgtttaa cgccattgga ccgcgagcgt gcattttctg cgtactgatg
     5281 cctatttcag cacccaatcc gaactgagcg ccatcggtaa aagcggtaga aacattggta
     5341 taaatgcagg ctgcatcgat cattgcatcg aataaggcaa tatttgcttc gttttcgctg
     5401 acaatacatt cactgtgttt tgagccataa cagttgatgt gttgaatggc ttcctgaagg
     5461 gactcaacag ttttgatggc cattttgtag tcaaggaact cggtgccgaa actttcttgt
     5521 gtagcttttt gaagcaaatg cacgggatat tttcctacaa gaaaagtata ggctttggcg
     5581 tctgcatata taactacatt tttttctgcc agttttttac ataacgctgg gaggtcgctc
     5641 aatcgctttt cgtgaataat caaacaatcg agcgcattgc agacacttac tcgtcgggtt
     5701 ttagcattga gaattatttt gctgcctttt tctgtattgc caaattcatc aaaataagta
     5761 tgacaaatgc cggctccggt ttcaattacc ggaatttttg agtgtgtccg tacaaagtcg
     5821 attaatcctt ttcctccacg tggaataact aaatctacat atcccgttgc attgagcaaa
     5881 tcagctgtgg cttcgtgccc agatggcaac aaggtaatga tatccggatt tagaccaaaa
     5941 cgatttagaa cttcacgaat caggctgaca atggcagcat tggaatggta tgcatcactg
     6001 ccacctttca gtacgcagga atttcccgat ttcagacaga gtgaaaaaac atcaaaagtt
     6061 acattgggac gagcttcgta aacaatacca atcacgccaa acggaacggt aatttttgaa
     6121 attttcatgc catttgggcg aacagtttcc atcaatgttt ttcccaaagg actaggaaga
     6181 ccggcaacat ttcgaatatc tgccgctata ttttgaagcc gctgttcgtt gagcagaagc
     6241 cgatcgtaca taggatatgc cttttccata gcggcgaggt cttctgcatt tgcttttaaa
     6301 attttttccg aaccgatagc tatctcatcg gcaagggcaa gtaaggtttc ggaaattgct
     6361 ttatcgtcga ccagttgaag ggtgcgggca gcatttaggg cagcctcttt ccagtcgtaa
     6421 gaatgattgg tagcgttcat catcaaaaaa ttttataggt tgagagctaa aagttaactg
     6481 aatgactgca aaatgaacat gcaaagagtt tacattctga agtcgaaacg ttcactgcgg
     6541 gtattttcat aaatttcccg atcgaaggta taatacctgg ccggtttgtg ggaaactccg
     6601 ctttgcaatt cgtccgtttg aatcagataa ggcattttgt tgaccttgcg tctgaaattg
     6661 cgtttatcgt atgttgtgtc aaaaattgct tcataaactt tttgcatttg agtcagtgta
     6721 aatttttcgg gcaaaagttc aaatattagt ggttcgtgca gcaaatcgta acgtaatttt
     6781 ttcaaagctt cgttaaaaat gtcattgtga tcaaacatca attcccgaat ttcataagca
     6841 ttttcccatt ttgcttcttc aagcagtttg ctattgtcca catcatgaat atttaccagc
     6901 gaataaaatc caaccgttaa aactctgtca tcctgcaagc gcggttttac ccattcgtaa
     6961 tctctttcgc gtcgcttgag gcggtcagga tcactaaaaa ccataaactg tttcatgaaa
     7021 atattttcaa gcccggtttg ctctttcaaa atacgagaag cggttgcatc tatttgctcg
     7081 tttcgacgga cgtggttgcc gggcaatttc cattcgatga gaatatttcc ttcaaatgga
     7141 acatattccc ttcgcaccag tactttcagt aaattgcctt cgtaaccgaa aataacacaa
     7201 tcaaccgaaa cgcttggcag agtttggaac tctctattct ctaaaacgtt ttcaaattcg
     7261 ggcatgataa aaacaatata atttttaacc cgacaaagtt aagaaaaaaa gatatttttc
     7321 gctaaaaaat tttttctcaa aaaattgaaa atcacggttt caaaaaaaaa tttttgtact
     7381 tagtgtaaaa aaaacacatt tttatttgga gaataaaatt ttagcctcta tatttgtcct
     7441 tgaaaatttg ttttagctac ataaaaattt aatcaattat ttttttaaaa ctattaatat
     7501 catgaaaaac aaaaggttaa atctgtttta cctcctttta ttcttcattc cgattttttc
     7561 tgctattagt gctcaggaat ccgtcagcga tcgtgttgaa gaattgcttt ccaaaatgac
     7621 tattgaggaa aaagtcgggc agatggctca gataactttg gatgctattg gcaacggagc
     7681 taccgttttt gaaagcaaaa taccttttgc cattgattcg gtgaggttgc ataaagccat
     7741 agtcgattat cacgtcggct ccgtaatcaa taccacaaac aattacgctt tgagtgcgct
     7801 tcaatggaat agtattattg accaaattca gaaaacagca acaactaaaa caactctgcg
     7861 aataccggta atttacggaa tagatgctat tcacggggta acttacacct ccggagctac
     7921 tcttttcccg caacagattg ccatagccgc cacttttaac ccgggaaatg cctataatat
     7981 ggcaaaagtt acagcttatg aaacacgagc ttcaggcatt gcgtggaatt tttctcctgt
     8041 gctcgactta ggggctgatc ctcgattctc acgacaatat gaaggatttg gagaagatcc
     8101 ttatttggct tcggttatgg gaaggcaagc aataagaggt tatgaaggaa ttaccgatag
     8161 cattgggcat gcagaaaaag tagctgcctg tgccaaacat tttttaggtt attcggttcc
     8221 tttttcgggg aaggatcgca cgcccgccta tattcccgat catattctca gagaatatca
     8281 tttgccttcc tttaaagctg ccatcgatga aggagtagaa tcggtcatga tcaattcagg
     8341 aattattaat ggagtttcgg ttcatgccaa ttacaatatc attaccaaac tgcttaaaga
     8401 agaacttgga tttcagggtg ttgtggtaag cgactggatg gacattatca atctcgaaaa
     8461 aagggataaa attgcaaaaa acaataagga agccattgct ttggctatca acgcaggcat
     8521 agatatgtcg atggttcctt acgattacga acagttttgc aaggatttga cagcgcttgt
     8581 gaaggaggga aaagttaaag aaagcaggat agatgatgcc gttcgtcgaa ttttaaagtt
     8641 aaaattcaaa ctcggtttat ttgaaaatcc tgttacaaat ttgagcaatt atcccgactt
     8701 tggttccgac aagcatgaaa aattggctta caaggctgcc gctgatgcca ttacgttgct
     8761 gaagaacaaa aacaatattc ttccgttgaa caaaaatatc aagatattgg taaccggtcc
     8821 caatgccaac agcatgagaa gtttaaatgg aggatggagt tattcgtggc aaggtgaaaa
     8881 aaccgaacgt tatgctgctc aataccatac tattttggaa gctgtacagc aaaaaattgg
     8941 cgaaaagaac gtcctttatg agccgggcgt aagttatgtg gaaacgggtc aatattttga
     9001 agagaaagaa aacggtttgc aaaaagcagt tgctgcggct cgtaaggtag atgccattgt
     9061 actttgttta ggggaaaatt cttatgcgga aaagccggga gatttaaacg atttgaattt
     9121 gagcgaactg caaatcaaat tggccaaatc gctttcggca gtcaataaac ctattatttt
     9181 ggttttgaat gaagggcgtc cgcgttgctt cagtgctatt gaatccaaaa tgaatgccat
     9241 tgtacaaact tatcttccgg gcaattacgg aggcgatgca ttggccgata ttctttttgg
     9301 agatgtcaat ccttcgggaa agcttccata tacttatccg gcttatccta attcgatagt
     9361 gaactattat cataaatttt cggaagaaca aaaaccttcc gaaggagctt ataaatatga
     9421 atcggattat aatccacagt atgagtttgg attcggactt agttacacta catttgccta
     9481 cagcaatttg aaaatttcct cttcggttct ttatcccaac gaattaattg aagtcagcgt
     9541 ggacgttacc aatactggaa ccagagaagg aaaggaaacg gtacttttgt ttaccagtga
     9601 tgtaatagcc agtataacac cggatatgag aagattgcga cggtttgaaa aaatcaattt
     9661 gaaacccaat gaaacgaaaa cggttaaatt tactctgtcg gccaatgact tggcttttgt
     9721 taacacggat aaccaatgga caattgaacc gggagaattt gaaattaaaa taggcaatct
     9781 caaaaaaaca ataaattatc aatcaacaaa ttaaccaact aatttatcaa tcaatggaaa
     9841 atgtaaagaa aaattccgga aaaaaaatcg tgtctcggta tgttacgcga tttttaaaaa
     9901 gatggttgcc cttactggtg attattgtta tgccgctggc attgtcggcg caaaaaacaa
     9961 cgattacagg agttgttgtc gacagcaata acgagcccgt tattggtgca actgttttga
    10021 tcaaggggac tactacggga accgttacca attacgaagg gaagttcacc atcgaggcat
    10081 ccagcaatga tgtgctggta gttagctata ttggaatggt tccgcaggaa attaaagtgg
    10141 gaaattcaaa aaacatcaat gtagtgctga aagaagacat taaaattctt tctgaagttg
    10201 tagtagtggg atatggaacg caaaagaaaa aagatttaac tacggcagta tcgaccgttt
    10261 ctgctgaaga ttttaatgtt cgacccatcg tttcggcagg tcaggcaatt cagggaaaag
    10321 ctgcaggggt tcaggtaatt caacctaatg gagcgccggg aggagaaacg gtgataagag
    10381 tacgtggaac gacttctttt aatgggaata gtgacccatt atacgttgta gatggagtgc
    10441 cggttgataa tattaatttc ctttcaccca atgatatcga gaatatcaat atacttaaag
    10501 atgcttcttc ggcagctatt tatggctctc gtgcagcaaa tggagttgtt attattacta
    10561 caaaacaagg caagtcgggt gagtcgaaaa ttgcgtttaa tgcccaattg acagccaaaa
    10621 atgttactaa taagatagaa tctcttaatg ccgctgatta taaggattta atggaagagg
    10681 ttcgcccggg ttcgtcttct caaatgagtt ccgatgatat aaccaattgg tatgatgaag
    10741 tttaccgcac cgggttgcaa caaagttatc agctttcttt ctcaaatagt actgaaaaaa
    10801 cgagttattt tctttctgcc ggctaccttg atgagaaagg tattttccct gcatcttttt
    10861 ttaagcgtta ctctttcaga gcaaatatga ccaatgagat aagaaaatgg ctgagtattg
    10921 gttccaatat atcgtattca gataattcca acaatggcat aactaccggt gctggttcaa
    10981 atcgaggcgg agtagtgttg gctttaatta atcttccaac aagtttaccg gttatcaatc
    11041 ctgaaacagg atattataat cgtagttttt atggtcagaa cattaccaat cccgtagaag
    11101 caatggaaaa cgggaaagac gatattacgc gtgaaaaccg attaatagct tcaggtaatg
    11161 ccatgataac ttttatgaaa ggaatgaatt tcaagtcaac ttttacgctc gacagaaggc
    11221 atgggttaca aacgggtttt agtcctgtga tacacgttac aggtattgac gaatatggaa
    11281 gcgcatggga caatagaaat atgaatacgg ttctgacttg ggataatatt tttaattacc
    11341 agaaatcgtt tgataagaat aatattgaat tgatgatggg ttcgtcttgg actgattcaa
    11401 agtacaccaa tagttggata aacggttctc attatagaaa caacaaaata aaaacgctta
    11461 atgttgccaa caaaatatcg tggaataata cgggaaccgg gggctctaca tggggaatta
    11521 tgtcttactt tggacgtgtt tcgtacaatt acaacagtaa gtatttaatt actgccaatt
    11581 tcagggctga tggttcttct cgtttacatc ccgatcatcg atggggatat tttccttctt
    11641 tttcggctgc atggcgtata tcgtccgaag aatttatgca ggggctcaat tggctcgacg
    11701 acctcaaaat acgtggtggt tggggacaaa cgggtaatca actcgggctc ggcgattatg
    11761 cctatttgca gagatattcc gttacccgtc aacaatggtg gataaccggt caggaaaata
    11821 ttttaccaat cattactgcc agcaatttgc ggactacaga cttgacttgg gaaactacaa
    11881 ctcagtcaaa tgtgggtatt gattttacgg catttaagag caggttgaat attattatgg
    11941 attattatta caaaaagacc accgatatgt tgctcaacat tactttgcct tcgggggcta
    12001 atgtaacaag tattcagcgt aatggcggat taatgcgcaa tacaggtttt gaactttccg
    12061 taaactcaat caatatcacg aataaaaatt tcaagtggac ttcagatttg gaattttcgc
    12121 tcaatcggaa taaactggta aagcttgatt taacaaagat ttattatgac gctgctactt
    12181 ccgatgcttt tcatgggatt cagattgtac gcaatcagga aggacgttct cttggcggtt
    12241 tctacggcta cattagcgat ggggtagatt cagaaacggg ggaacttatc tatagagata
    12301 tcaatggaga caataaaata actgcgtctg accgaacttt tattggagat cccaatcctg
    12361 attttattta tggatttacc aatacattca catggaaagg tttcagtctt agtattttct
    12421 tgcaaggaac ttatggaaac gatattttca atgcttcaag aatagaaacg gaaggaatgt
    12481 atgatttaaa gaaccaatcg acgcgtgttc tggatcgatg gagacgtccg ggtatgataa
    12541 ccaccattcc caaagccaac ttcaatcttc aaccttcgtc ctattttgta gaagatggaa
    12601 gttatttaag attgaaagat ttgacattat cctataatgt aacggctcca tttttgaaaa
    12661 aatcgggaat ctctcggtta caaccttata taactgcttc caatttattg acttttacaa
    12721 aatatttagg tatggatcct gaagtcaatg agtggggtaa ttcaaacgct gttcagggaa
    12781 ttgactgggg aacatatcct caagcaaaat cctttgtttt tggttttaat attgaattct
    12841 aaaaaattta ttactatgaa aataataaaa tatatactga tttccgctgt ggtatttgtt
    12901 gcagcatcat gctcgctcga ttataacccc attgatacct attcggatat tactgaagga
    12961 attgatacca catctgcagc cagtcaagtg attttaaaag ataaggctgc cgttctgaat
    13021 caacgacagg tactttatca gcgactgaaa gacggtcagg aacattggta tcttgatatg
    13081 ttgttgttgg cagaatctca ttccgataat gcttacgccg gatccccaaa tgcggaaaca
    13141 acaccttttg aaataaattc tattgaagga tctaatccaa atcttaaacg tgattggaat
    13201 ggacacatgg ataatattgc ccaagccaat cgacttattc aaggcattga cgagttgaaa
    13261 gatccttcct tgacggaagc agaagctaaa caaattcgtg ccgaaggact tatttttcgc
    13321 tcactggttt acttcgatat ggtcagaatt tggggtagag tgcctcttat aactactgta
    13381 ggaaaagaca taacggccga taatatcgaa gaagtttatc ctgcgtattt tcctcagcaa
    13441 agtgaagaaa ttgatgttta tagacagata gaagaagatt tgttggaagc agtacgatat
    13501 gcacccaaca atgatccgag caataaaacc ctacttacca aatcggtggc aagagctctt
    13561 ttggcaaaag tgtatgccga gaaaccattg cgtgattaca ataaagttat cgcttatgcc
    13621 gattctgttg cagccgatgg ttttgaattg gtttccgact tcagcgattt gtggggtgtg
    13681 caacttcaaa atccttctgc acctccgggc gaaaacaatt ttgccattga tgcaaaaaaa
    13741 agaaacacat cggaagccat tcttgaaatt cagtatcctg ttggatcagg gaattgggta
    13801 acatggatgt ttggccgttc gcttgacaac tggaatttct ggttcgattg ggctaaatgg
    13861 ataactcctt ctcgcgattt gatcaatgct tttcaggctg ccggcgacat aaaacgttat
    13921 aacgaaagtg tggtttggta ttcttgcagt tggaatattt attatccttc caacaattat
    13981 ccatttatgt acaaaacccg ttcggcctat aacagcatta ttaaaattcg ttacgccgat
    14041 attctgttgc tgaaagctga agcgttaatt caaaccggag acttgagcgg tgccgcttca
    14101 attataaatg ataaaattcg caaacgcgca gggcttgctc ccttgcctgc ttctgtaaca
    14161 aatgacaaaa atgcgttgtt gagtgcttat ctgaatgaac gtcgtcttga attggctttc
    14221 gaaggacaac gctggtttga cttggtaaga ttggagaaag tggaagaagt tttgaataca
    14281 cttccttcaa gagattcagg tcgccctgcg ctccgttatc cttacgataa atactcatat
    14341 aaactcccta ttcctcagga tattatagat cagaatccaa atttggtaca aaatccgggt
    14401 tattaataat taaaattcta ttcaaatgaa aaatataatc aaactaacaa tattcggact
    14461 gctggggtta ttcgcaacat cttgccagca agaagaactg agagtgcatt atcctgcctc
    14521 caaacccgtt tttgacagca ttagcgtacc ggaaaccaat attgtttatg gcgattccat
    14581 cacgttatat gtaggcgttt ccgatcctct gactccatta tctacgctcg aaataaaagt
    14641 ggttgtaaac gacgaactca ttgcttccga aacgatcaga acaaaaggga atcaggcaac
    14701 ctatcatcaa cgttacggaa ttccttttgt ggcgcgtatg cccgataatg cagaagtgga
    14761 aattcatttg acttccatta atgtggaagg aaccaaaacg gatagcattt acgtaaatac
    14821 catagccaaa cgccctacta ttacggatct ttatttggtt gttccactta aatcgcctgt
    14881 caaaatgaaa ttgaccgatg ctcaaaatta tatttatacg gctgacaatt taagtctgga
    14941 aaatactgtt tctttccgac tggctaccaa attgaaatat ggaataagca tcgattggac
    15001 aggtttggta ttcggcaaag taggaaatgg aattggattg atcgatatta atggaagtcc
    15061 cattactctg tctgatgcaa ctttgatagg ttataagaaa attacggttg atctgttcaa
    15121 ctttactgtt aaaggagaag gcgacaagct gcaaccggct acagcaatgg atatcagtac
    15181 tttcggaact gtttcgcttt cgtcgacaga ttacctgaat gttactacca aagaagactg
    15241 gaaaacgaaa aaaatgtatc ttgggaaagg caccgaaatg accattagcg gtattccaac
    15301 gacaaatctt gccaataatc tggctccgga tttctttgag gttacaggct cgaatacggc
    15361 aaaattcctg ggcgaaaccg gcatttatac ggtttattac ttgcccagac tcaattttgt
    15421 atttgttgaa cagcccgatg ccgtatatcc ggatgccatg tggctttgtg gggtaggttt
    15481 tggcccagct cgaacgccac atataaaaac ttcttcttgg aattggaaca cgccgctcga
    15541 atatcagttc tgcagaaaag tttccgatgg cgtttatcag gcaacattct atgcacttca
    15601 tcaaattgac gatactgcag cagaaccctg gagattgaag ttcagtgtga aattcttcca
    15661 tcaaagaatg tggggcggag aagaagatgc ccgcacttac acattaaatg tcaatggttt
    15721 gctgtttgct ccaactgaaa gcgatcaagg taattttgcc ggaaccgatg cgtttaagga
    15781 taaacccggg atttatcgac ttaccattga tgtgaacaat aagacggcta actttcaaaa
    15841 aataagtgat taattattta catccatccc gtaaacaact tttttgcggg atggataatt
    15901 tttttttgag atgaacaaaa aaagcatatt cgtattttta tttcttcttt gggtttttat
    15961 tttttctacg ggatgtaagc attcgggaga tgaaccggtt cctactccta cacctcagga
    16021 ttcggtaggg gatgtaagtg tatggttgac caccggcaat gaatcttgtc tgttaaaaaa
    16081 aatggcaccg attaattttt ccagagattt tgccgatttt accattgtca tcgacaccac
    16141 aacaacttat caatatattg atggttttgg tgctgcgttg accgggtctt cagcacattt
    16201 aatccatcaa atgagtgccc aaaacaggaa aaaacttttg gacgatctgt tcgaccctga
    16261 aaaaggaatc ggtatcaatt atttgcgaat aacaattggt tcttccgatt tttctatagg
    16321 gacatacagt tattgcgaca gtcccgatat taataaattc gatattcccg aaatagaccg
    16381 aaatgaactt ttgccggtat tgaaagaaat tcttgaaatt aatccatcga tgaaaattct
    16441 ggccagtccc tggagtgcgc cggcatggat gaaaagtaat aacagcatgt atggcggaag
    16501 tttgaaaggt gaagaagttt acaatgactt tgcagaatat tttgttcgtt atgttcaggt
    16561 ttttgaacag gaaggcattc atattgatgc gctgaccatt caaaacgagc ctatgcatga
    16621 aacaggagga tatccgacca tgaaaatgct ttggcaggaa caaaacaaaa ttattcgcga
    16681 ttatttaggc cctaagttta ttgccaacgg cattcaaacc aaaattataa tttgggatca
    16741 caatttcgat atgtcctatt accccatcaa tattctaaat gatccggtta cccgacaata
    16801 tgtaaatggc accggctggc atggatacaa aggaaatgca tccgcaatag atcaggtaac
    16861 tgcactttac cccgataagg aaacttattt caccgaaatc tcgggtggag gatggaatac
    16921 cgaaacccgc atcaaaaata tgttttatta catgaaagat ttccttatgg ctacggtcaa
    16981 tcgtggctct aaaaattttc ttatgtggaa tctcgcttta aatcctcaac atggaccaac
    17041 taccacaact tggggtggct gtcaggattg cagaggtgtg gtaactatca acgaaaatga
    17101 gacttacatt cgcaacgagg agtattattt gctgggacat tttgctaaag tggtacgttt
    17161 gggagctgta cgtgtaaata attcctgtta caatttacct tccggcatga ccatatcttc
    17221 ctttatgaat gccgatggca gcaaagtggt ggtcattttg aacagaagcg ggtcatctcg
    17281 gaaatataat gttcgctgtg gaacccgaaa attttactat cagcaagtag atgaatcggt
    17341 tgtatcattc aaattctgat tttttatcga gaaattattt tttttgttaa ataaaaaagt
    17401 ttatccatga aaataagagc tttatttttt ttaatggcct ttgccattgt taccaattgc
    17461 atttcggcag ggaaagtaac tgtttggtca tcgctgcccg ataccaaagc tttattggta
    17521 aaaaagagca tgaagttgaa ttctaaacct gtagaaatta aaaacaaggt tgaaattttt
    17581 cccgatcaaa cctgtcaatc ggttgaaggt gttggagctg ccctcaccca ttcttcagct
    17641 tatgttttcc atcacaatct gacagcccaa agaagggatt cattgtttaa ggtgcttttt
    17701 actcctgcag gtatcggcat caattatacg cggctttgca ttggtgcttc cgactttgcc
    17761 tttaatcttt tcagttacag cgaaacggaa gatttttcgt taagtaattt taatattcag
    17821 gaagatgaaa aagatgtaat tcccatgctg aaggaaattt tggccatcaa tccaaacttg
    17881 caaattttgg catcaccctg gagccctccc ggatggatga aaacttccgg aagtatggtt
    17941 ggcggcaaat tgcgtcccga ttgctacgat gtgtatgccg aatatttgat caaatacatt
    18001 gaggcttatc gacaacatgg gataaccatt catgcgctta cggtgcagaa cgaaccggaa
    18061 tacggaactg cagcttatcc ttgtatggat atgacggcaa aggaacaaca gatttttatc
    18121 cgttattttc tgggccccaa aatgcagaaa aaaggaattc agaccaaaat cattcttttc
    18181 gatcacaatt gcgacaaccc ggactatccg ataagtattt taaatgatcc cgatacaaag
    18241 aaatttgtag atggttcagg atttcatctt tataaaggag aaatatctgc gctttgtaaa
    18301 gttcatgaag cacatcccga taaaaatatt tatttcaccg aacaatcggg tggaggttgg
    18361 gctcccgact ttgatcaaaa cgttcgttgg tatgttggag aattgtttgc cggcgccatg
    18421 aactgctgga gtaaaaatgt tctcctttgg aatttggctt tggacgaaaa cgacggacca
    18481 aaaaatttcg gttgccaaaa ttgttggggc gtagtacgcg ttacttcgga tggaagaatc
    18541 gaaaaaaatg ctgaatttta tgccattggt cattatggga aagtggtaca accaggagct
    18601 gtccgactcg tttcaaatca ggatatttta aaaggcgtgg cttttcgcaa tcctgataac
    18661 agttttgcct atctggccgt ttattatggc acaactccgc aaaccatagg tattcagtgt
    18721 ggaaacagtt cttttgttta tacttttaaa cccggagaag tagtatcttt tctgtggtag
    18781 atgtaagaaa ttgctatcgg ttgcagttca gataaaaatc tgtccaagcc gatagcaatt
    18841 ttttttgtga attttcgagc gaaactttcg ctgaatatgt ttctaaaatg taatttttct
    18901 caagaaagca attttttttt taccatttcc gaaatcgctt tcccatctgc ttttccctcc
    18961 aacttgcgag tggcaattct cactactttt cataaatttt gagcccctgt gcactggttt
    19021 gggctatgat ttatctgaaa gttactctct gaaaatctca gcttactttt ttacacctgt
    19081 aagtccaatt ttgaaaaaac gcaatcaaag gaattttatt tccttaaaat aatatttggc
    19141 tgaggttcct ttttcggttt cgagttccaa agcgccatcc ggataaatgc ggctgatttt
    19201 ggcccgaaat atttctgtcc ccgcctgata aaaatggtag ccatttttgc gatacaagga
    19261 ttgggcataa acacttctga ttttttcgga ttcccactgc ccgtacactt ccaaaattga
    19321 cgcaacaatt cgatgcagca actttttccg attaaaactt ttgccggcaa tttgtttcag
    19381 agaaatcgga tggggtgcgt cgctgaaaaa ttcggtctga ttgacattca agcctacacc
    19441 tattacagct tttgatattt tgccccgaaa aagggaagtt tcgatcagta ttccggccag
    19501 cttgctatct ttccaataca tatcgttggg ccatttgatg gtaatatcgt ccgaaactgt
    19561 gtccaagaca ttttttattc ccacactcac cgcttgcgaa atcagaaact gctcttcaat
    19621 aggaatatga tgcgggtaca gtactatgct gaaaagcaga ttctttccgc gagccgattc
    19681 ccaagaattt cctgcctgtc ctttccctgc tgtctgataa tctgcaacca caacaaatcc
    19741 ttccggaata tctctttctt tcaacatttt ccaacacaac acattggtgg ataccgtttg
    19801 aggaatataa aaaacatttt ccgtgagcat gcaaaacaat ttatttgaaa accgacagca
    19861 acagttcttc gatttttttg gttaccgctc ttgaagttcc ctgcgcagtg tttaccatga
    19921 tgacaaaagt ataggttttt ccttcgtttt ttacataacc ggcataacat ttcacttttt
    19981 caatgcttcc acttttggca aaaactttcc cttccaatgg cgtaccggcc aaaaacgaaa
    20041 caagagttcc tgttttccca gcaatgggta aagaattgaa aaaatcttta ctgaaagcac
    20101 ttttggaata catgtaatcc agcaactcgg taataaactg agctgaaaca gcattcatgg
    20161 gtgaaagtcc agaaccgtcg cacataaaga gctgatctat gggaagtcct ttcaatttcc
    20221 acaaattacg tatggtttta actccgcctc tgccggtaac gggaacacct tcaccaagtc
    20281 ccaaataacg aaaaagacat tctgcataat gattattgct tctttcgttg gtttgcttga
    20341 taatatctct cagtggagga gaaaaatgcg tataaacaac ttgcccatca aaaggcgttg
    20401 aaaattcggc aacaggagga ttattaactg taatgccact ctggatcaac ttatcggaaa
    20461 actgctttgc caaaagcaaa accggattgg gaatatcacc ttttactaca aattctgctc
    20521 tgttggctgg aatttcgcca taaagccgac gcaaattgga attgggcata ccgcaaaaat
    20581 aggcactgtc gaacgtaatc tttgaacttt ttaaataatt ttctatcacc aagtccggca
    20641 aatcgggaag agtgcggata atggcaggcg tggtcccaat cggtccggaa cgtaaaacca
    20701 aacgaaaagt attgtcgaga taagaaattc cgaaaatgga aggagcataa taatttccaa
    20761 tatcttccca tatccagaaa ggactgactc cttcttggtt gtaaatactg gcatcggcta
    20821 taatttttcc ttcaatttta ctgattcctg cttgttttat agccaacacc cattggtcaa
    20881 gaaaattttt ttctcccaaa aattccgaac ccagtgtggg gtcacctgca cctttaataa
    20941 ttagattccc ctgtaaaaca tgttctttgg ttacattgcc ggaaatctgc agtttcgttt
    21001 gaaatctgaa attcgggccc agcaattcga gagccgaagc tgtagtaacc agttttaaag
    21061 tagaagccgg aataatgctt ttgttggcat tgtaacgtcc caccactttc ccttttcccg
    21121 tttcgcgtat cagaaggctt acattggcat tcttcaacaa aggatgatga ataaaattat
    21181 cgacaggagt ttgagcgaca aggttgagca caaaaaagct aacaaaaacg ttgacaaaaa
    21241 ttttcttcat aaaatagaaa tttcagatca tgttgattta tagataaatg tatatctcac
    21301 tgtaatgctg taaaaaataa atggtaatag aagcatattg tttgccgtga cctgttctat
    21361 agcaatagtt aatctgcaca aaaacaacca aattatgttc ccaaaccatt tcaatattta
    21421 tttctcaata aatttttatt ttgtacaaat tcgaccctta tcttgggtat tgagtcaaaa
    21481 gtagaagttt caaatgtaca atattttttt aaatttgtca ttccttcccg tgaattaaaa
    21541 atatagcaat tatcgtttca cagcaaagag aatgatttta atattttttc attgattaaa
    21601 gttttgctta ttttcaactt aaaaattgcg tcaagttagt gttataatta aatttaatct
    21661 tgtaactttg cgaaggttcg ttatctttac tgaaaaaagt atgaaaaatt ttactttcct
    21721 gttgttgttt tccgtttttg gcggggaact tttatgccat gcttcaggag aacaagcaaa
    21781 tataatttca ctaaaaaatg attctttgct gttttcatca gatcagatcg agcttgatgc
    21841 tttcgattct attcctgaaa aaaacataaa ggaagtagtt gtatatggca tttcttctgg
    21901 tagattgact tttccacttt cgttggtaag taaaacatca cttctgaata gttcttttac
    21961 cacccctgcc gatgcgttgc aaagagaaac cggcattttc atgtcccgcg atggaatttg
    22021 ggctacttca gttaatatca gaggtttgtc cgaacaaaga cttctttttc tggtagacga
    22081 ag
//
LOCUS       DAOSCH010000020        21898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1700_length_21898_cov_5.829236, whole genome shotgun sequence.
ACCESSION   DAOSCH010000020 DAOSCH010000000
VERSION     DAOSCH010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21898
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1700_length_21898_cov_5.829236"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(304..561)
                     /locus_tag="PLZ54_02900"
     CDS             complement(304..561)
                     /locus_tag="PLZ54_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65917.1"
                     /translation="MNEKQTKNAAESIVKALVELSLGKEPNIFSKSPFRKLAEHKNYT
                     LIRDAYIDYLKEFDGKIDSEEDMKRLFDFRIKILNYFNDDK"
     gene            complement(574..2322)
                     /gene="aspS"
                     /locus_tag="PLZ54_02905"
     CDS             complement(574..2322)
                     /gene="aspS"
                     /locus_tag="PLZ54_02905"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOJ65918.1"
                     /translation="MYRTHNCGELRISHVGQIITLAGWVQRIRKMGGMVFIDLRDRYG
                     ITQLVFNQEINSDLWEKANKLGREFVVQVTGKVSERSNKNPNLSTGDIEILVSELTVL
                     NESKTPPFTIEDATDGGDDLRMKYRYLDLRRNVVRKNLELRHQMTIETRQYLDNLGFL
                     EVETPVLIASTPEGARDFVVPSRMNPGEFYALPQSPQLFKQLLMVGGFDRYFQIVKCF
                     RDEDLRADRQPEFTQIDCEMSFVEQEDVLQTFEGLVKHLFKTIKNIDFPEPFPRMEWS
                     EAMKRFGSDKPDIRFGMELVELKEMVTGHDFSVFDNAEYVGGICAPACASYTRKQIDE
                     LTEFVKRPQIGAKGLIYIRFENDGSFKSSIDKFFSVDDLKKIASLFSANPGDLILIMS
                     GEKLKTQKALCELRLEMGGRLGLRKKDEFAPLWVIDFPLFEWNEELNRYQAMHHPFTS
                     PKPEDIPLLDTQPGEVRANAYDMVVNGVELGGGSIRIHNSNLQNKMFELLGFTPEKAQ
                     AQFGFLMNAFQFGAPPHGGLAFGLDRFVSLLAGLDSIRDCIAFPKNNAGRDVMINAPS
                     PLDPSQLEELHLMLKL"
     gene            2557..2991
                     /gene="ruvX"
                     /locus_tag="PLZ54_02910"
     CDS             2557..2991
                     /gene="ruvX"
                     /locus_tag="PLZ54_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOJ65919.1"
                     /translation="MGRILAIDYGQKRVGIAVSDVMHISANGLTTLPVNEVFPFLEKY
                     LVQENVETIVVGFPRQMNGKESDSMKFIRPFVAKLVQKFPQIELIYEDERFTSVLAHK
                     TMLEGGLKKKGRQNKALVDKISATIILQSYLESVRYKNERNI"
     gene            3019..3570
                     /gene="def"
                     /locus_tag="PLZ54_02915"
     CDS             3019..3570
                     /gene="def"
                     /locus_tag="PLZ54_02915"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOJ65920.1"
                     /translation="MILPIYTYGNPVLRKVAQPIDASYPELKSLVNNMFETMYHADGV
                     GLAAPQVGLPIRVLVIDLAPFKDEDAELGSFKIAMINPTILERSAELLPGEEGCLSIP
                     GIHETVFRSKTIKVHYFDTDFNEYEEIFEDYKARVIQHEYDHLEGNLFTDRVNPLRRQ
                     LLKSKLTNISKGKIQCNYKIITA"
     gene            complement(3597..4901)
                     /locus_tag="PLZ54_02920"
     CDS             complement(3597..4901)
                     /locus_tag="PLZ54_02920"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOJ65921.1"
                     /translation="MNYEQTLDYLYSRLPVFHQIGSAAYKPGLENTIRILTALHQPQN
                     SFRSIHVAGTNGKGSVSHFLSSILQSSGYKTGLYTSPHLVDFGERIRVNGEMIDKQFV
                     IDFVENHKKLIEEVQPSFFEITMAMAFDYFASSKVDVAVIEVGLGGRLDSTNIIQPEL
                     SVITNISFDHEEFLGHTLQEIATEKAGIIKPHTPVVIGEALPETKPVFLQKAQEMNAP
                     IFFSENIKQIFFDRYEENRMIVKTSDGKSYTVGLTGNYQLKNIATVLTAVDQLRKANF
                     EIPEASLQEGLKKVIEKTGLQGRWQIISASPKIIADTGHNPGGIAFVAEQLKTQHYQT
                     LRIIFGMANDKDIDTVLSLLPKNGVYYFTQANIKRAFPSDQLLKRGQQAGLYGKSFSN
                     IEEAIKAALDEANKEDLIFIGGSNYVVGEALTFFEKHEEVTQ"
     gene            complement(4956..5498)
                     /locus_tag="PLZ54_02925"
     CDS             complement(4956..5498)
                     /locus_tag="PLZ54_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ65922.1"
                     /translation="MESFLDLVKRRQSAHSFTNKPVEKEKIDRILEIVRLSPSACNAQ
                     PWKFIVVTDEEKRQEIADATSKRFLPVNHFSKQAPVHIVIVEESTNFFTKVGCYGVNR
                     QYPLIDIGIATAHLCLAATNEGLDTHVIGWYNEKKIQQALEIPENKKVMLVVALGYSD
                     EEVRPKKRKKMEELVSFNKY"
     gene            5651..6979
                     /gene="tilS"
                     /locus_tag="PLZ54_02930"
     CDS             5651..6979
                     /gene="tilS"
                     /locus_tag="PLZ54_02930"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOJ65923.1"
                     /translation="MILKKVELYIEKNKLLFPEKGKILVGVSGGRDSVALLDILIKLG
                     YQCTVAHCNFHLRGEESDRDEKFVQQLSFNLNIPYYLVDFDTVNYAKQKNISIEMAAR
                     ELRYSWFTSLAEKINAQAIAIAHHADDNVETLLMHLVRGTGLKGLTGISPKNGLIVRP
                     LLCCSRNEINEYIKNNNLSFIEDSTNQSVDFQRNKIRLQVIPLLEEINPSVKKVLSES
                     IERFSEINTFYENAIEKIKKQLLTVDNDQLKINIDLLCKQASPKTILFEILHPYGFNE
                     SIVQDIEKHLHDESGKIFYSPTHYLIKDRKYLIISNKIKKNETTFFITENDSEISFPL
                     HLSITRKKKEKNFVISKDNRIIQIDASLIQYPLTLRRWTYGDTFFPFGMNRQKKLSDF
                     FIDQKLNLKQKAETWLLLSQNQIVWVVGLRLDNRFKITESTQEVLEIKML"
     gene            7208..9070
                     /gene="rho"
                     /locus_tag="PLZ54_02935"
     CDS             7208..9070
                     /gene="rho"
                     /locus_tag="PLZ54_02935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOJ65924.1"
                     /translation="MSNYNILQLNAKELSELKQIASELGLKVPGTITKDKLVYEILDQ
                     QAIANAQQKVASNEKKENERKKRMRVSVKKNVEQKVFSSPNPTITPAPSNSTTSPTPL
                     VPFTENSPKQEIQNNQTSDNTIEAPASTTEVKPKKSGGQSKRNKTSKSNQKTLLKKTS
                     AETSEKPEVNIPLEEIDNFEVPNLPEVEITEEQDIDIEKDTLIPVQIIETSEPRPGLS
                     LNINKNANGNNNENKVKIAPRQDKTEKQFDFDGILEGTGTLEIIQDGYGFLRSPDYNY
                     LSSPDDIYVSQSQIKLFGLKTGDTVTGTIRPPKEGEKYFPLIKVLKINGRSPEYVRDR
                     VSFEHLTPLFPNEKFKLTTGVNDPLSVRIVDLFSPIGKGQRGLIVAQPKTGKTVLLKD
                     IANAIAANHPEVYMIVLLIDERPEEVTDMARSVKAEVISSTFDEPAERHVKVASMVHE
                     KAKRMVECGQDVVILLDSITRLARAYNTVAPASGKILSGGVDANALHKPKRFFGAARN
                     IENGGSLTIIATALTETGSKMDDVIFEEFKGTGNMELQLDRKLANKRIFPAVDILASS
                     TRREDLLLDQDTLNRMWILRRHLSDMNSLEAMEFLKERMERTVNNEEFLVSMNA"
     gene            9129..9983
                     /locus_tag="PLZ54_02940"
     CDS             9129..9983
                     /locus_tag="PLZ54_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ65925.1"
                     /translation="MNKLFRLGIVSFLIVSLFTSCEKENNQVTSVTISETSKTMHIGD
                     IDTLYAQSQYEGNIVPSLAWSSSDSSVVSVDKNGEISAQKKGNAIITASAGDKSATCN
                     VTVDDEIVTSFTKAELAFWGDYYQTGNSNNYSLYLTNRQDTLLIEINTDTLSTNGIPS
                     GTYEMIENFDSLQQIVPLTLLPAFIYDGYSYGSFLFNSVAVYPIYSGIATFSNEENSN
                     TITYNFLDYFGNHIAGEYSGTIIFYDETENPSAVASNVKAIKMNKKIHQFSSNRKISK
                     VLKLINAR"
     gene            10096..10842
                     /gene="fabG"
                     /locus_tag="PLZ54_02945"
     CDS             10096..10842
                     /gene="fabG"
                     /locus_tag="PLZ54_02945"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOJ65926.1"
                     /translation="MKLLEGKVAIVTGAARGIGKSIAIKLAQEGADIAFTDLVIDENA
                     SNTQKEIESLGVKVKAYASNAANFEETHAVVEEIQKEFGHIDILVNNAGITRDGLLIR
                     MTESQWDSVINVNLKSAFNFIHACAPIMMKQRNGSIINMSSVVGVSGNAGQANYSASK
                     AGLIGLTKSVAKEFGSRGIRANAIAPGFIETEMTHALTEEQRAKWVENIPLRRGGTPE
                     EVAKVTLFLASDMSSYVSGQVIHVCGGMNT"
     gene            11054..14356
                     /gene="secA"
                     /locus_tag="PLZ54_02950"
     CDS             11054..14356
                     /gene="secA"
                     /locus_tag="PLZ54_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOJ65927.1"
                     /translation="MALNNFFKKILGNKAQRDLREIEPIVEKIQAVYPEIKSLSNDDL
                     RARTASLKERIQEYVAPEVNRIQELKNSIEETEIDLREKIYTEIDKLEKEITDKQEKI
                     LNEILPEAFNIMKETARRFAQNDEIIVKANNFDRNLAAKHDFVYIDGEEAHYKNEWMA
                     GGSMIKWDMVHYDVQLIGGVVLHQGKIAEMATGEGKTLVATLPVFLNALTGNGVHIVT
                     VNDYLSKRDSEWMGPLFMFHGLSVDCIDKYRPNSEERRQAYLADITYGTNNEFGFDYL
                     RDNMATSPLDLVQRKHHYAIVDEVDSVLIDDARTPLIISGPVPKGEDQLFEEFRPTVE
                     RLVNAQKVLATKYLTDARNLLKSENEKEREEGALALFRSYKGLPKNKALIKFLSEPGN
                     KTILLKTEEYYMQENNRNMHIATDPLYFVIDEKNNSIELTDKGFDLLSETSEDPTFFV
                     LPDVGSEIAELEKNHTLTPEEKQAQKDEILQNYSIKSERIHTVNQLLKAYTLFEKDVE
                     YVVINNEVKIVDEQTGRIMEGRRYSDGLHQALEAKERVTVEAATQTFATITLQNYFRM
                     YHKLAGMTGTAETEAGEFWDIYKLDVVVIPTNRPVIREDMEDRVYKTKREKYAAVIDE
                     IVRLMEAGRPVLVGTTSVEVSELLSRMLTGRKIKHNVLNAKQHQREAEIVAEAGRKGT
                     ITIATNMAGRGTDIKLTPEVREAGGLAIVGTERHESRRVDRQLRGRAGRQGDPGSSVF
                     YVSLEDDLMRLFGSERISSVMDRLGFQEGEMIQHPMISRSIERAQKKVEENNFGIRKR
                     LLEYDDVMNSQREVIYTRRRHALMGERIESDITNMIYDAIENMVANAKSNGFYEELEE
                     QLLTVFAMDVPFSEEEFNSARTRDLTDKVVNAALTTFKRKMEKLSSIAYPVIKQVYEQ
                     QGDKYENILIPVTDGKRIFNVVVHLETAYKTEGKEVIKSFEKQTLLYVIDDEWKEHLR
                     QLDELRNSVQNATYEQKDPLLIYKLESFGLFKEMIENINHKVLSILMRGQIPVREPEK
                     VREAKIPARQDFSKYKTQKDEIAERGNSNAGDTREKQRPQPVHVEKKVGRNDPCPCGS
                     GKKYKHCHGANA"
     gene            14388..15278
                     /gene="lgt"
                     /locus_tag="PLZ54_02955"
     CDS             14388..15278
                     /gene="lgt"
                     /locus_tag="PLZ54_02955"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018695276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOJ65928.1"
                     /translation="MLNYIVWNVNPEIFRLGSLSVRWYGLLWAVGILLALLIGQKIFK
                     HEKLPEVWLDKIFIYAVIGGIVGARLGHCLFYEWKLLPEPVQFLGITFKYGNYYLSHP
                     WEILYVWHGGLASHGGAIGILIAMYFYNKNVTKKGYLWAFDRLAIGVAITGACIRLGN
                     LMNSEIYGNPTNLPWGFIFVRAGETQPMHPTQIYEMLYCLITFGVIWWLYWKKEAYKK
                     TGFIFGVFLIGIFGTRFLLEFIKLDQEAFESGMLLNMGQILSIPFIVWGIWLVARKQK
                     ITPTSDVNANKNKPVANRKK"
     gene            complement(15293..16309)
                     /gene="holA"
                     /locus_tag="PLZ54_02960"
     CDS             complement(15293..16309)
                     /gene="holA"
                     /locus_tag="PLZ54_02960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOJ65929.1"
                     /translation="MIKQEAAADEILQEIKSKNFHSVYFLMGEEPYYIDKISNYIQEN
                     MLSESEKDFDLTILYGKDVEISTVINTAKRFPMIAPYQVVIVKEAQMISSWENLVYYL
                     QKPLKSTILVFCYKYGTPDKRTKWFQETKKAGVVFESGKLYDYQVGEWIRSYLKTRKT
                     GITQQAENMLVDYLGTDLTKIANELDKLLLTKPQDTQQITPELIERNIGISKDYNVFE
                     LQKAFVNRDILKANRIILYFAENKKNNPLVLVLAQLFNFFGNLLLFHYMKDKTEKNIA
                     SELKINPFFVKDYQNAAKIYDREKTIKVLSLIRETDARSKGVDNAGIDEGELLKELVY
                     KILH"
     gene            complement(16353..17126)
                     /locus_tag="PLZ54_02965"
     CDS             complement(16353..17126)
                     /locus_tag="PLZ54_02965"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOJ65930.1"
                     /translation="MKTKKEIVENWLPRYTKRPLEEFTPYILLVNFNIYVELFAGYYQ
                     VPIVGKDGNMPNASANGITIINFGLGSPNAALIMDILSAIQPKAVLFLGKCGGLRDKN
                     KEGDYILPIAAIRGEGTSNDYFPPEIPSLPAFSLQRSVSSNIRDIGKDYWTGTVYTTN
                     RRVWEHDEKFKEYLRMTRAMAIDMETATLFSAGFYNGIPTGALLLVSDMPMYESGIKT
                     AESDKRVTANFVEEHLRIGIESLTSIMHNSRTIKHLRFE"
     gene            17210..17668
                     /locus_tag="PLZ54_02970"
     CDS             17210..17668
                     /locus_tag="PLZ54_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOJ65931.1"
                     /translation="MLQLNLPSYNFKIKKVNETLYIFDSQRKKYVKLTPEEWVRQNFI
                     RFLLEEKHFPSAYLAVEKELVFNGMKKRCDAVLYDGKAQPLMILEFKAPHVLLTQSVF
                     DQVAVYNYRLNVEYFIVSNGLEHYCCRVKKEHTKFEFLKEIPDYTNFLNQ"
     gene            complement(17698..18609)
                     /locus_tag="PLZ54_02975"
     CDS             complement(17698..18609)
                     /locus_tag="PLZ54_02975"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOJ65932.1"
                     /translation="MVDLKVDIGKLHLKNPVMTASGTFGYGMEYSDFIDISRIGGIIV
                     KGTTLRKREGNPYPRMKETASGMLNAVGLQNKGVDHFIEHIYPQLTNVDTNIIVNVSG
                     STIEEYVETAEKLNNLEKIPAIELNISCPNVKEGGMLFGTSCTAAKEVVAKVRMAYKN
                     VLIVKLSPNVTNISEIAQAVEEAGADAISLINTVLGMAIDAETRKPCLSTVTGGLSGP
                     AIKPLALRMVWETARDVKIPVIGIGGIMNAADAIEFMLAGASAVQVGTANFIDPTVTI
                     KIIEGIEDYLNRHGYTSVSDIVGALET"
     gene            complement(18603..19373)
                     /locus_tag="PLZ54_02980"
     CDS             complement(18603..19373)
                     /locus_tag="PLZ54_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOJ65933.1"
                     /translation="MKKLILDLILKKNIRLNEQVYLLIFSSEKGLPEILPGQFAQVKI
                     NDSPATFLRRPFSVNFVDYENNELWLLIQVVGAGTLKLSETSPGASVNMILPLGNSFS
                     FPEKKDEKILLIGGGSGIAPLLFFGSWLFDAGFSPEFLIGARSSIHLVEVEEFKKYGN
                     VYITTEDGTEGEKGFVTDHSILKKRNYDRIYSCGPTPMMKAVGAYAENNNIFCEVSLE
                     NTMGCGIGACLCCVTDTTQGRVCVCTEGTVFNIEKLKW"
     gene            complement(19479..19964)
                     /locus_tag="PLZ54_02985"
     CDS             complement(19479..19964)
                     /locus_tag="PLZ54_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ65934.1"
                     /translation="MDLKDRIEKIMQNEAMNAVQFASEIGIQGSTLSHILNGRNKPSL
                     EVVMKILKRFDYINSDWLIFGEGPMSRTERQSKTPTLFDSQDLSNTNTVNYTQKTEQK
                     IESLQSHFQQKPEQKEIISESVNSKISEENKIAPVVVEKIIKRIIVYFNDNTFQEFTT
                     T"
     gene            complement(20895..21671)
                     /locus_tag="PLZ54_02990"
     CDS             complement(20895..21671)
                     /locus_tag="PLZ54_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOJ65935.1"
                     /translation="MATEPKTEKEISVEEKLRALYELQRVYSKIDEIRNLRDELPLEV
                     QDLEDEIIGLNTRLENYKNEIAELETAIKAKKLEIEQARTKIEKYKEQQENVRNNREY
                     DNLSKEIEYQTLEIELCEKRIKEYTAALEAKKAEQQSTMEALSERRLDLDQKKGELNE
                     IISETKQEEERLREQIKQIEQRVEPRLLTAFKRIRKNSRNGLSVVPVQRDACGGCFNK
                     IPAQRQLDIRSRKKIIVCEYCGRILVDPELAGIVPPTSES"
     gene            complement(21677..>21898)
                     /locus_tag="PLZ54_02995"
     CDS             complement(21677..>21898)
                     /locus_tag="PLZ54_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOJ65936.1"
                     /translation="LKDAVQQGADIYISADFKYHEFFNAEGKILIADVGHYESEQFTK
                     DIFYEIISKKIPNFVVKISETYTNPINYL"
BASE COUNT         7180 a         4005 c         3843 g         6870 t
ORIGIN      
        1 atttctattt aatttctatt tatttatgaa ttaagtaatt tcaaacgata tttttcaaaa
       61 aataattttg caaataactt agaaactctt tgaacaaaat tttattgatc attcatttaa
      121 aaaactctgt tttgctgcaa ttttgatatg atatttgtta gtatggaata ttatttgaaa
      181 tcacactatt gtaaactatt tcagaataaa ataaatttgc ttaattttcc tataatttat
      241 attatgtcaa ataagaattg atgaaattct ttcttaatac tctatcctaa aactgaaata
      301 tttttattta tcgtcattga aataattcaa aatttttatg cggaaatcga aaagacgctt
      361 catgtcttct tccgaatcaa ttttgccatc gaattctttc aaataatcga tataagcatc
      421 tctgattaaa gtataatttt tatgttcggc caattttcta aatggtgatt tggaaaaaat
      481 atttggttct tttcccaaac taagttcaac caaagcctta acaatcgatt cggcagcgtt
      541 tttggtttgt ttttcattca tggcaaatgt tttttaaagt ttgagcatca aatgtaattc
      601 ttccagttgc gaaggatcca atggtgacgg agcatttatc attacatctc ttccagcatt
      661 attttttgga aaagcaatac agtcccttat cgaatcaagt ccggccaata aagaaacaaa
      721 acggtcgagg ccaaaagcca aacctccatg cggaggcgct ccaaactgaa aagcattcat
      781 cagaaaacca aattgagctt gagctttttc cggtgtaaat cccagtaatt caaacatttt
      841 attctgcaaa ttgctgttat gaattcggat agagccccca cccaattcaa ctccattcac
      901 taccatatcg taggcattgg ctctaacttc accgggttgg gtatccaaca aagggatatc
      961 ttcaggtttt ggagatgtaa atgggtgatg catagcttga tagcgattta attcctcatt
     1021 ccattcaaac aatggaaaat cgataaccca aagaggtgca aattcgtctt ttttgcgaag
     1081 gcctaacctt cctcccattt ccaatcgtag ttcgcaaagt gctttttgag tttttaattt
     1141 ttctccggac atgataagaa ttaaatcgcc cggattggcg gaaaataacg aagcaatttt
     1201 tttcaaatca tcaacagaaa agaacttgtc gatagaagat ttgaaggaac cgtcattttc
     1261 aaagcgaata taaatcagtc cctttgctcc tatttgaggt cgttttacaa attccgtcaa
     1321 ctcatcaatc tgtttgcgtg tataggaagc gcaagccgga gcacatattc caccaacata
     1381 ttcggcattg tcgaaaactg aaaaatcgtg tcccgttacc atttctttaa gttcgacaag
     1441 ttccattcca aagcgaatat ccggtttatc ggaaccaaaa cgtttcattg cttcgctcca
     1501 ttccatacgt ggaaatggct ctggaaaatc tatattttta atggttttga acaaatgctt
     1561 taccaatcct tcaaaagttt ggagcacatc ttcctgttcc acaaaagaca tttcacaatc
     1621 aatctgtgta aattcgggtt gacgatcggc acgcaaatct tcatcacgga aacatttgac
     1681 aatttggaaa tagcgatcga atccgccaac cattaaaagt tgcttgaaca attggggaga
     1741 ttgaggtagc gcataaaact ctccgggatt cattcgggat ggaaccacga aatcgcgtgc
     1801 accttcaggt gtagaagcaa tcagcacagg agtttccact tcgagaaaac ctaaattgtc
     1861 caaatattga cgtgtttcaa tggtcatttg atgtcgaagt tccaaatttt ttctaacgac
     1921 atttctccgc aaatcgagat accgatactt cattcgcaaa tcatcgccac catccgtagc
     1981 atcttcaata gtaaaaggag gtgtttttga ttcattcaaa acggtgagtt cggaaaccaa
     2041 aatttctata tcgcccgtac ttaaattggg atttttatta cttctttcag aaactttacc
     2101 ggtaacctgt accacaaatt cgcgacccag tttgttggct ttttcccaca aatcggaatt
     2161 aatttcctga ttgaaaacca attgagttat accataccga tcgcgaagat caataaatac
     2221 catccctccc attttacgga ttctctgtac ccagccggct aaagtaataa tttgtccaac
     2281 atgagatatg cggagttcac cacagttatg agtcctatac atttattatt ctgataatta
     2341 ataaattaat aatactttta tttttaaagc tacaaacgaa aagaaaatta tctttatcat
     2401 tcaacaaaaa tacaaaaaat ccgtgtacct aaaccaatag acacaaaaat tcattttata
     2461 cgacaattaa aaataatttt agccataggt tgtgagtaag ttgaaaattt ttaaaatgaa
     2521 cactattttg taactttgca ttttcaaatg aagaaaatgg gaagaatact ggcaatagat
     2581 tatgggcaaa aaagagttgg aattgcagta agcgatgtga tgcatatttc tgcaaatgga
     2641 ctgacaacat tgccggtaaa tgaagttttt ccttttcttg aaaaatattt ggtgcaagaa
     2701 aatgttgaaa ccattgttgt aggttttcca cgacaaatga acggcaaaga atctgattcc
     2761 atgaaattta tccggccatt tgttgcaaaa ttggttcaga aatttcctca aattgaactt
     2821 atttacgaag atgaacgttt tacatcggtt ttggctcaca aaaccatgct cgaaggcggt
     2881 ttaaagaaaa agggcagaca aaacaaagcg ttggtcgata aaatttctgc aacaattatt
     2941 ttgcaaagtt atttggaatc agttagatac aaaaatgaaa gaaacatata acactcgaat
     3001 tacaataaaa aacaaataat gattcttcct atttacacat acggaaatcc cgttttacga
     3061 aaagttgcac aaccaattga tgcttcatat cccgaattaa aatctttggt aaacaacatg
     3121 tttgaaacca tgtatcatgc tgacggtgtc gggttggcag cgccacaagt tggtttaccc
     3181 ataagagttt tggttattga tttggctcca tttaaagatg aagatgcaga attgggcagt
     3241 tttaaaattg ccatgattaa tcccactatt ctcgaacgca gtgctgaatt gttgccgggc
     3301 gaagaaggtt gtttaagcat tcccgggatt catgaaacgg tatttcgttc caaaacgata
     3361 aaagtccatt atttcgatac cgacttcaac gaatacgaag agatttttga agattataaa
     3421 gcacgcgtta ttcaacacga atacgaccat ttggaaggta acctgttcac cgaccgagta
     3481 aatccactcc gacgtcagtt gctcaaatca aaacttacca atatttcaaa aggtaaaatt
     3541 cagtgcaatt ataaaattat tactgcttga aaatgatcga ataattgatt ttgaatttat
     3601 tgagtcactt cttcgtgctt ctcgaaaaac gttaaagctt ctccaacaac ataattgctg
     3661 ccgccgataa agattaaatc ttctttattt gcctcatcca aagcagcctt tatagcttct
     3721 tcgatatttg aaaaagattt tccataaagc cctgcttgtt gtcctctttt caataattgg
     3781 tcggaaggga atgccctttt aatgttggct tgtgtgaaat aatagactcc gtttttcggc
     3841 agcaatgaaa gtaccgtatc gatgtcttta tcgtttgcca ttccgaaaat tattcttagt
     3901 gtttgataat gttgcgtttt taactgttcc gcaacaaagg caattccacc gggattgtgg
     3961 cctgtatcgg caataatttt aggtgatgca gaaataattt gccatcttcc ctgaagtcct
     4021 gttttttcga taactttttt caaaccttct tgtaaagagg cttccggaat ttcaaaatta
     4081 gctttgcgca gctgatcaac agcagtcaaa accgttgcaa tatttttaag ttgatagttg
     4141 cctgtcaatc caactgtata agattttcca tcagaagttt tcactatcat ccgattttct
     4201 tcatatcgat caaaaaaaat ctgtttgatg ttttctgaaa aaaagatggg agcattcatt
     4261 tcctgtgctt tctgtaaaaa aacgggtttc gtttccggca aagcttctcc aataaccacc
     4321 ggtgtatgcg gttttatgat gccggctttt tctgtagcaa tttcctgcaa agtatgtcct
     4381 aaaaattctt cgtgatcaaa actgatattg gtaattaccg aaagctcagg ctgaataatg
     4441 ttcgtactgt cgagcctgcc tcccaaacca acttctatta ctgcaacatc aactttgctg
     4501 ctggcaaaat aatcgaaagc cattgccata gtaatttcaa aaaacgaagg ctgcacttct
     4561 tcaatcaatt ttttatgatt ttccacaaaa tctataacga attgtttatc aatcatttcg
     4621 ccgttcacac gaattctttc gccaaaatca accaaatgag gcgaagtgta caggccggtt
     4681 ttataacctg aagactgcaa aatggaagac aaaaaatgtg atacggaacc ttttccgttc
     4741 gttccggcaa catgaataga acgaaacgaa ttttgcggtt gatgcagggc tgtaagaata
     4801 cggattgtat tttctaatcc cggtttatat gcagcactgc caatttgatg aaatacaggt
     4861 aaacgcgaat aaagataatc taatgtttgt tcgtaattca ttaatttaca aataaatatt
     4921 cttatttaga ttttcacaaa aataagaatt ttcaatcaat atttattaaa ggaaaccaat
     4981 tcttccattt tctttctttt tttgggtcga acttcttcat cggaatatcc caaagcaaca
     5041 accagcataa cttttttatt ttcaggaatt tcaagagctt gctgaatttt tttttcatta
     5101 taccaaccta tcacatgcgt atccaaacct tcattcgttg cagccaaaca taaatgagca
     5161 gtggcaatgc caatatcaat cagtggatat tgacgattta cgccataaca gccaaccttc
     5221 gtaaaaaaat tggtgctttc ttcaacaatt acaatatgaa ccggagcttg tttggaaaaa
     5281 tgattgacag gaagaaatct ttttgaggta gcatcagcaa tttcttgtcg tttttcttcg
     5341 tctgtaacta caataaattt ccacggctgc gcattgcaag ccgatggaga aaggcgcact
     5401 atttcaagaa tccggtcgat tttttctttc tcaaccggct tgttggtaaa agaatgagca
     5461 ctctgacgcc ttttaaccaa atctaaaaat gattccataa tacatatttt tttaaattga
     5521 tcggcacttt tgttgcaatg acacgaaaaa aactatttgc tggaaattat atatctttgt
     5581 aaatctttga attcgagaaa aattttttat aagtttctat atttggcaaa gataatgtaa
     5641 ataaaagaaa atgatactca aaaaagtaga attatatatc gaaaaaaata aattactttt
     5701 ccccgaaaaa ggaaaaattt tagttggtgt gagtggtggt agagattcag tagcattact
     5761 cgatatttta attaaattgg gttatcagtg caccgttgct cattgtaatt ttcatttgcg
     5821 cggagaagaa tccgatagag acgaaaaatt tgtgcaacag ttatcgttca acttaaatat
     5881 tccttattat ttggttgatt ttgatacagt taattatgca aaacaaaaaa atatttccat
     5941 agaaatggct gcgcgcgaat tgcgttattc gtggtttaca tcattagcag aaaaaatcaa
     6001 tgcccaagcc atagccattg ctcaccatgc cgatgataac gttgaaacat tattaatgca
     6061 tcttgttcgt ggcaccggat tgaaaggcct gacgggaatt tctcccaaaa acggtctgat
     6121 tgttcgtccg ttgctttgtt gcagtagaaa tgaaattaac gaatacatca aaaacaacaa
     6181 tttaagtttt attgaagatt ctacaaatca atcagttgat tttcaaagaa ataaaattcg
     6241 acttcaagta atcccacttt tagaagaaat aaatccgtcg gtgaaaaaag ttctttccga
     6301 atccattgaa cgattttcgg aaatcaatac cttttatgaa aatgccatcg aaaaaataaa
     6361 aaaacaactg cttaccgtcg acaacgatca gctaaaaatc aatattgatt tgctgtgcaa
     6421 acaagcttct ccaaaaacta ttctgtttga aattttacat ccttacggct tcaacgaaag
     6481 tattgttcaa gatattgaaa agcatttgca tgacgaatcg ggcaaaatat tttattctcc
     6541 tacccattat ttgattaaag acagaaaata tttgattatc agcaataaaa taaagaaaaa
     6601 tgaaacaact tttttcatta cggaaaatga ttccgaaatc agctttcccc ttcatctttc
     6661 cataacaagg aaaaaaaagg aaaagaactt tgttatttca aaagacaatc gaatcataca
     6721 aatagatgcg tccttgattc aatatccact taccttgcgc agatggacgt atggtgatac
     6781 gtttttccct tttggtatga accgacaaaa aaaattaagt gattttttta tcgatcaaaa
     6841 gctgaattta aagcaaaaag ctgaaacatg gctgttgctt tcgcaaaatc aaatagtttg
     6901 ggtagtaggt cttcgactcg acaatcggtt taaaattacc gaatcaaccc aggaagttct
     6961 tgaaattaaa atgttgtaaa taagcgctca acaaattatc cttgaaaaat attttaataa
     7021 atttgtccat ttaacacaaa atgattaatt ttgcagcgta aaagcaaatc gatttaaaaa
     7081 agcattcctg ctttcattct ctacaatcaa aaatattttt attgcatttc tacgcaaaaa
     7141 atcttttttt attctgaatt ttattttgaa tatttgaaaa atcccaactt tatttttatc
     7201 caattttatg agtaattata atattttgca attaaatgca aaagaattat ccgagttaaa
     7261 acaaattgcc tccgaactcg gcctgaaggt acccggtacc attacaaaag acaaactggt
     7321 ttatgaaatt cttgatcaac aagccattgc aaatgcacag caaaaagttg catcaaatga
     7381 aaaaaaggaa aacgaaagga aaaaacggat gagggtttcg gtaaagaaaa atgttgaaca
     7441 aaaagttttt tcttcaccaa acccaactat cacacccgca ccttccaatt ccacaacttc
     7501 tccaactcct ttagttccct ttactgaaaa ttctcccaaa caagaaatac agaacaatca
     7561 gacttctgat aacacgattg aggctccagc ttcaactact gaagtaaaac ctaaaaaatc
     7621 gggcggacaa tcgaaacgca acaaaacttc caaatcaaac caaaaaacct tgctaaaaaa
     7681 gacttctgct gaaacgtcgg aaaaacctga agtaaatatt cctttggaag aaattgataa
     7741 ttttgaagtc ccgaatcttc ccgaagtcga aattaccgaa gaacaggata ttgatattga
     7801 aaaagataca ctgattcctg ttcagattat tgaaacttcc gaaccacgtc ccggactttc
     7861 tttaaatatc aacaaaaatg ctaacgggaa taataatgaa aataaggtaa aaattgcacc
     7921 tcgacaagac aaaaccgaaa aacaattcga ttttgacgga attctggaag gaaccggaac
     7981 acttgaaatt attcaggatg gctatggatt tctgcgttcg cccgattaca attatctttc
     8041 ttcccctgat gatatttacg tttctcaatc tcaaatcaaa cttttcggac tgaagaccgg
     8101 cgatacggtt acaggcacca ttcgtcctcc caaagaaggg gaaaaatatt ttcctttaat
     8161 aaaagttctg aaaatcaatg gtcgttcgcc tgaatatgtg cgcgatcgtg tttcttttga
     8221 acatttgaca cctcttttcc ccaacgaaaa atttaaactg actaccggcg ttaacgatcc
     8281 tctttccgta cggattgtag atttgttttc tcctattggt aaaggtcaac gcggactaat
     8341 tgttgcgcaa cccaaaaccg gaaaaacagt tttgttgaaa gacattgcca atgccattgc
     8401 cgccaatcat cccgaagttt atatgattgt tttgctcatc gatgagcgac cagaagaagt
     8461 tactgacatg gcacgcagcg taaaagccga agtaatatca tctactttcg acgagccggc
     8521 cgaacgccac gtaaaagtgg caagcatggt acacgaaaag gcaaaacgca tggtagaatg
     8581 tggacaagat gtggtcattt tgctcgattc cattacccgt ttggctcgtg cttacaatac
     8641 agtggccccg gcttccggaa aaatactttc cggtggcgtg gatgccaatg ctcttcacaa
     8701 gccaaaacga ttttttggag ctgcaagaaa tatagaaaac ggtggaagcc tcaccataat
     8761 tgcgacagcc cttactgaaa caggttcaaa aatggacgat gttatttttg aagaatttaa
     8821 aggtaccggc aatatggaat tgcaactcga ccggaaattg gccaacaaaa gaattttccc
     8881 cgctgtggat attctggctt ccagcactcg acgcgaagat ttactgctcg atcaggatac
     8941 tttgaacaga atgtggattt tgcgtcgcca tctttccgat atgaactctt tggaagcaat
     9001 ggaatttttg aaggaacgca tggaacgtac cgtaaacaac gaagaatttt tagtttcaat
     9061 gaatgcttga tttatcataa cttgtaattc aatatcttat ttctataacc aataattttg
     9121 taataaaaat gaataaatta tttagattgg gtatcgtctc ttttcttatc gtttccctgt
     9181 ttacatcttg tgaaaaggaa aataatcagg ttacctctgt aactattagc gaaaccagca
     9241 aaactatgca tattggcgat atcgacacac tctatgccca atctcaatac gaaggtaata
     9301 ttgttccatc tttggcttgg agttcaagcg attcatcagt agtttcagta gataaaaacg
     9361 gagaaatatc ggcccaaaaa aagggaaatg ccattattac ggcttctgcc ggcgataaat
     9421 cggctacatg caatgttacg gtagatgatg aaatcgtaac ttcatttaca aaagcagaat
     9481 tggcattttg gggtgattat tatcaaacag gcaattccaa taattactcc ttatatctta
     9541 ccaacaggca agatactctg ttaattgaaa tcaacaccga tactctttct accaatggaa
     9601 ttccgtcagg aacttacgaa atgattgaaa attttgattc attacaacaa attgtccctt
     9661 taactttact tccggctttc atatacgatg gttattctta tggttcattt cttttcaatt
     9721 ctgttgcagt ttatccaatt tattcgggaa ttgcaacttt cagcaatgaa gaaaattcta
     9781 atacaattac ctacaacttc ttggattatt ttggaaatca tatagccggt gaatattcag
     9841 gaacaattat tttttatgat gagacagaaa atccatcagc agtagcttca aacgtgaaag
     9901 ctataaaaat gaacaaaaaa attcatcagt tttcatcaaa ccgtaaaatt tcgaaagtat
     9961 tgaaattaat caatgcaaga taattttata caatcatatt taataatttt cgttttgaac
    10021 aatcgtttca agaatcgtta ataaatttta caatactcga aaaaacaaaa ttaattattt
    10081 caaaaacaat tttttatgaa attattagaa ggaaaagtag ctatcgtaac cggagctgca
    10141 cgcggaatag gcaaatccat tgccatcaaa ttggctcaag aaggagcaga tattgctttt
    10201 accgatttgg ttatagacga aaatgcgtca aatacgcaaa aagaaattga atctcttggt
    10261 gtaaaagtga aagcttatgc gtccaatgct gccaattttg aagaaacgca tgctgttgta
    10321 gaagaaattc aaaaagaatt tggccatatc gacattttgg tcaataacgc cggtatcacc
    10381 agagacggtt tattgattcg tatgacagaa tctcaatggg attcggttat taatgtaaat
    10441 cttaaatctg cttttaattt tattcatgcc tgcgctccca taatgatgaa acaaagaaat
    10501 ggcagtatta tcaacatgag ttctgtagta ggcgtttcag gaaatgcagg acaagccaat
    10561 tattctgctt ccaaagccgg attaatagga ttgacaaaat cggtcgcaaa agaatttggc
    10621 tcacgcggta ttcgtgccaa tgccattgct ccgggattca tcgaaacaga aatgactcat
    10681 gctttgactg aagagcagcg tgccaagtgg gtagaaaata ttcctttacg tcgtggtgga
    10741 actcccgaag aagttgccaa agttacactt ttccttgcat ctgacatgtc gagttatgtt
    10801 agtggacagg ttattcatgt ttgtggtggc atgaatactt aaaaccaaat atccttttat
    10861 atatttcttc aaacgcttct gacaacggaa gcgtttttgc tttttatcaa ctataagaag
    10921 aatttttata cattatccat ctggttttaa ctttaaaaaa atgtatcttt gcaaaacttt
    10981 ttgcttacag atatgtttaa gcacgagtca ttttatttat tcatcagaga gcgtataaaa
    11041 aatttacaaa ataatggcat taaataattt ttttaaaaaa atactgggaa acaaagcaca
    11101 acgtgactta agagaaattg agcccatcgt tgaaaaaatt caagctgttt atcctgaaat
    11161 caaatctctt tcaaatgacg atctcagagc aagaacagct tccttgaaag aacgtattca
    11221 agaatacgtc gctccggaag ttaatcggat tcaagaatta aaaaacagca tagaagaaac
    11281 ggaaatcgac cttcgagaaa aaatttatac cgaaatagat aagctcgaaa aggaaataac
    11341 cgacaagcag gaaaaaattc tcaacgaaat tcttccggaa gctttcaata taatgaaaga
    11401 aactgctcgc cgttttgccc aaaacgatga aattattgta aaagctaaca attttgatcg
    11461 taatcttgca gccaaacatg attttgtata cattgacggt gaagaagccc attacaaaaa
    11521 tgaatggatg gcgggtggca gcatgattaa atgggacatg gttcactacg acgttcagct
    11581 tattggtggc gtggtattgc atcagggaaa aattgccgaa atggccacag gtgagggaaa
    11641 aacactggta gctactttgc ccgttttttt aaatgctctg accggtaatg gtgttcatat
    11701 cgttacggtg aacgattatt tgtccaaacg cgactccgaa tggatggggc cactatttat
    11761 gtttcatgga ttatctgtag actgcatcga taaatatcgt ccaaattccg aagaacgccg
    11821 tcaggcttac ctcgctgaca taacctacgg aaccaacaat gaattcgggt ttgattactt
    11881 gcgcgataac atggcaacga gccctttgga tttggtacaa cgtaaacacc attatgccat
    11941 tgtcgacgaa gtggactctg ttttgattga tgatgcaaga acacctttga ttatttccgg
    12001 ccctgtaccc aaaggagaag atcagctttt cgaagaattt cgccctactg tagagcgttt
    12061 ggtaaatgca caaaaagtgt tggctaccaa gtatcttacc gatgcccgta acttgctgaa
    12121 atcggaaaat gaaaaggaac gtgaagaagg tgctttggct cttttccgct cctacaaagg
    12181 tttgccaaaa aataaggctt tgattaaatt tttgagtgaa ccgggaaata aaaccatttt
    12241 gctgaaaacg gaagaatact atatgcagga aaacaatcgg aatatgcaca tagctaccga
    12301 tcccctgtat tttgtaattg atgaaaaaaa caattctatt gaacttaccg ataaaggatt
    12361 tgatttactt tcggaaactt ctgaagatcc cacatttttc gttttaccgg atgtcggaag
    12421 cgaaattgcc gaactggaaa aaaatcatac gctgacaccc gaagaaaaac aggctcaaaa
    12481 agatgaaatt ctgcaaaact attccattaa atcagaacgc attcacaccg tcaatcaatt
    12541 actgaaagct tatactttat ttgaaaaaga tgttgaatat gtggttatca acaatgaagt
    12601 taaaatagtt gatgaacaaa ccggacgtat catggaagga cggcgatatt ctgacggact
    12661 gcatcaggca ctcgaagcca aagaacgcgt taccgtagaa gctgccacac agacttttgc
    12721 caccattaca ctgcaaaatt acttcagaat gtatcacaaa cttgccggta tgacgggaac
    12781 tgccgaaact gaagccggag aattttggga catttataaa cttgatgtgg tggttattcc
    12841 taccaatcgt cctgttattc gcgaagatat ggaagaccgc gtttacaaaa caaaacgtga
    12901 aaaatatgct gctgttatag atgaaattgt tcggctgatg gaagccggta gaccggtatt
    12961 ggttgggaca acatctgtag aagtatccga attattaagc cgaatgctca ccggtagaaa
    13021 aataaaacac aacgtgttaa atgccaaaca gcatcaaaga gaagccgaaa ttgttgctga
    13081 agccggacgc aagggaacaa taaccattgc taccaatatg gcaggtcgtg gtacggatat
    13141 taaacttact cctgaagtta gggaagccgg aggattggcc atcgttggta cggaacgaca
    13201 tgaaagccgt cgtgtggatc gtcagttgag aggacgtgcc ggtcgtcagg gtgatccggg
    13261 ctcttccgtt ttttacgttt cgttggaaga tgatttgatg cgtctgtttg gttctgaacg
    13321 tatttcgagc gtcatggacc gacttggctt tcaggaagga gaaatgattc agcaccctat
    13381 gatttcccgt tccatcgaaa gagcacaaaa gaaagtagaa gaaaataact ttggcatccg
    13441 taaacgatta ctcgaatacg atgatgtgat gaattctcag cgcgaagtta tttatacccg
    13501 tcgtcgacat gctttaatgg gagaaagaat agaatcggat atcaccaaca tgatttatga
    13561 tgccattgaa aatatggtgg ctaatgccaa aagcaacgga ttctatgaag aacttgaaga
    13621 acaactgttg acggtttttg ctatggatgt tcctttttcc gaagaagaat tcaattctgc
    13681 ccgaacacgc gatttgactg acaaagtggt taacgcagca cttactacct tcaagcgaaa
    13741 aatggaaaaa ttgtcttcca tagcctaccc cgtgattaaa caggtatatg aacaacaagg
    13801 agataaatat gaaaacatcc tgattcctgt aactgatgga aaaaggattt ttaatgttgt
    13861 tgttcatttg gagacagctt acaaaacaga aggcaaagaa gtaatcaaat cttttgaaaa
    13921 acagacttta ctttatgtga ttgacgacga atggaaagaa catctgcgcc agttggacga
    13981 actgcgaaat tcggttcaaa acgcaactta cgaacaaaaa gatcctctgt tgatatataa
    14041 actcgaatcg ttcggattgt tcaaagaaat gattgaaaat atcaaccaca aggttctctc
    14101 cattttgatg cgtggacaaa ttcctgtaag agaaccggaa aaagtcagag aggcaaaaat
    14161 acctgcacgt caggatttca gcaaatataa aacgcaaaag gacgaaattg ctgaaagagg
    14221 aaattcaaat gccggcgata ccagagaaaa acagcgtccg caacctgtcc atgtggaaaa
    14281 gaaagtggga agaaatgatc cctgtccgtg cggtagtgga aaaaaataca aacactgtca
    14341 cggagcaaat gcttaaaaat ttaatcccat aaaagaatcg aatcggcatg ctcaattata
    14401 tagtttggaa cgttaatccg gaaatatttc gtttaggttc attgtctgta cgatggtacg
    14461 gattactttg ggctgtgggt attttgctgg ctttactgat aggtcagaaa atttttaaac
    14521 atgaaaagct tccagaagtt tggctcgaca aaatttttat ttatgcagta attggcggta
    14581 ttgttggggc acgtttgggg cactgtctgt tttatgaatg gaaattactc cccgaaccgg
    14641 tacaattttt aggtattact ttcaaatacg gaaattatta tttgtcgcat ccctgggaaa
    14701 ttctatatgt gtggcatggg gggctggcaa gtcatggcgg agcaattggc attctgatag
    14761 ccatgtattt ttacaataaa aatgttacca aaaaaggata tctttgggca tttgaccgtt
    14821 tagctattgg tgttgccatt accggtgcgt gcataaggct cgggaatttg atgaattcgg
    14881 aaatttatgg aaatccgacc aatcttccgt ggggttttat ttttgttcga gcgggcgaaa
    14941 cgcagcccat gcatccaact caaatttatg agatgcttta ttgcctgata acatttggcg
    15001 taatttggtg gttgtactgg aaaaaagaag cctataaaaa aaccggattt atttttggcg
    15061 tttttttgat aggtatattc ggaacacgtt ttttactcga atttattaag cttgatcagg
    15121 aagcttttga atcgggtatg ttattgaata tggggcaaat tttaagcatt ccatttattg
    15181 tttggggcat ttggctggtg gcaagaaaac aaaaaattac gccaacatcc gatgtaaatg
    15241 caaataaaaa caagcctgtt gccaatagaa aaaaataatg ttataaactt gcttagtgca
    15301 aaattttgta aaccaattct tttaatagtt ctccttcgtc aattccggcg ttgtcgaccc
    15361 ctttgcttct tgcatctgtt tctctgatta gcgaaagtac ttttattgtc ttttctctat
    15421 cataaatttt agctgcgttc tggtaatctt ttacgaaaaa aggattgatt ttcagttcgg
    15481 aggcaatatt tttttcggtt ttatctttca tgtaatggaa caaaagcaaa ttcccaaaaa
    15541 aattgaaaag ctgcgccaat acaagtacca acggattatt ctttttattt tcggcaaaat
    15601 atagaataat tcggttggct ttcaaaatat ctcgattgac aaatgctttt tgaagttcga
    15661 aaacattata atccttgctg atgccaatat tcctttcaat taattccgga gttatttgtt
    15721 gggtatcttg tggcttagtt aaaagtaact tatccagctc attggcaatt tttgtcaaat
    15781 ctgttcccaa ataatcaacc agcatatttt cagcttgctg ggttattccc gttttacgag
    15841 ttttaagata cgatcgtatc cattcgccta cctgataatc atacagtttt cccgattcaa
    15901 aaacaactcc tgctttcttt gtttcctgaa accacttggt tcgtttatcg ggtgttccat
    15961 atttgtaaca aaaaaccaaa atagtcgatt ttaatggttt ttgaagataa tataccaaat
    16021 tctcccatga acttatcatt tgcgcttcct tcactattac tacctgatag ggagcaatca
    16081 tgggaaatcg tttcgccgta ttgatgaccg tcgaaatttc aacatctttt ccatagagaa
    16141 tcgttaaatc gaagtccttt tcactttctg aaagcatatt ctcctgtatg taatttgaaa
    16201 ttttgtcgat ataatacggt tcttcaccca tcaaaaaata aacggaatga aaatttttcg
    16261 attttatttc ctgcaaaatt tcatcagctg cggcttcctg ttttatcact ttaacaaaaa
    16321 tattttatca gttttataat ggcaactaat tgttattcaa atcttaaatg cttaatggtt
    16381 ctgctgttgt gcataatgga tgtaagcgat tctattccga ttcgtaaatg ttcttccaca
    16441 aaattggccg ttactctttt atcgctttct gccgttttaa tccccgattc gtacataggc
    16501 atatccgaaa ccagcagcaa tgcaccggta ggaatgccgt tataaaaacc ggcagaaaac
    16561 aaagtggctg tttccatatc gatagccata gctcgcgtca ttcgaagata ttccttaaat
    16621 ttctcgtcat gctcccatac tcttctgttg gtcgtgtaaa cagtgcctgt ccaataatct
    16681 tttccaatgt cgcgaatatt tgaagatacg gagcgctgta agctgaatgc aggcaaagag
    16741 ggaatttcgg gagggaaata atcatttgaa gttccttctc cacgaatagc cgcaataggc
    16801 aaaatataat ctccttcctt gtttttatct cttaatccgc cacatttccc caaaaataat
    16861 actgctttgg gttgaatggc cgacaaaata tccataatca aagctgcatt ggggcttccc
    16921 aatccgaaat tgattatggt aatgccattg gctgaggcat tgggcatatt tccatcttta
    16981 ccgacaatag gaacttggta gtaaccggca aaaagttcca cataaatgtt gaaattaacc
    17041 agcaagatat atggagtaaa ttcttcgagc ggtcgcttgg tatagcgggg aagccaattt
    17101 tctacaattt ctttttttgt tttcatagtt atttcagcat ttgttttgta acttcgtaag
    17161 cttaaactgc tccataaata aaaatttaat cgggacaaaa ttaggaaaaa tgttgcaatt
    17221 aaatcttcct tcttacaatt tcaaaataaa aaaggtgaat gaaacccttt atattttcga
    17281 cagtcagaga aaaaaatatg ttaagttgac ccctgaagaa tgggttcgtc aaaacttcat
    17341 acgttttctt ttggaagaaa agcattttcc ttctgcctat cttgctgttg agaaagaatt
    17401 ggttttcaat ggaatgaaaa aacgctgtga tgcagtttta tacgatggga aagctcaacc
    17461 tctaatgatt ttggaattta aagctcccca cgttctcctt actcaaagtg tgttcgatca
    17521 ggttgctgtt tataattacc ggctaaatgt agaatatttt atcgtaagta acggtttgga
    17581 gcattactgt tgccgggtga agaaggagca tacaaaattt gaatttttga aggaaattcc
    17641 cgattacacg aattttttga atcaataaaa ttagaaaaaa gcttgtttta aaaactttca
    17701 ggtttccaat gctccaacaa tatctgaaac cgaagtataa ccatgacgat tgagataatc
    17761 ttctattcct tcgataattt ttatggttac cgtaggatca ataaaatttg ctgttccaac
    17821 ttgaacggct gaagccccgg caagcataaa ttctatggca tcagcagcat tcatgatacc
    17881 acctatacca ataactggaa ttttaacgtc tcgagctgtt tcccaaacca ttctcaacgc
    17941 caatggcttg atggcaggtc cggataatcc ccccgtaacc gttgaaaggc aaggttttcg
    18001 agtttcggca tcaatagcca taccgagcac cgtattaatt agcgaaatag cgtctgcacc
    18061 ggcttcttcg acggcttgcg caatttcgga aatgttggta acatttggcg aaagcttaac
    18121 aattaatacg tttttatatg ccattcgcac tttagcaaca acctccttag ctgctgtaca
    18181 tgaggttcca aagagcattc cgccttcttt tacgtttgga caggaaatat tgagttcgat
    18241 ggcgggtatt ttttcgagat tattcaattt ttcggccgtt tctacatatt cttcaatggt
    18301 agaacccgac acattcacaa ttatatttgt atcgacattt gttaattgag gatagatatg
    18361 ttcaataaaa tgatcaacac ccttattttg cagtcccaca gcatttaaca tgcctgaagc
    18421 agtttctttc atgcgaggat aagggtttcc ttcacgtttg cgaagtgttg ttccttttac
    18481 aataatacca cctattcgtg atatatcaat aaagtctgaa tactccattc catagccgaa
    18541 agttcctgat gcagtcatta caggattttt caaatgtaat tttccaatgt caacttttaa
    18601 atctaccatt ttaatttttc aatattaaac accgttccct ctgtacaaac acaaactcga
    18661 ccttgagtag tgtcggttac acaacataaa catgctccaa tgccacaacc catagtattt
    18721 tcaagtgaaa cttcacaaaa gatattatta ttctcggcat aagcacctac agctttcatc
    18781 atgggagtgg gcccacaaga gtaaattctg tcataattcc tcttttttaa tatagaatga
    18841 tctgttacaa atcccttctc gccttcagtg ccatcttctg tagtaatata gacgtttcca
    18901 tattttttaa attcctccac ctccaccaaa tgaatactac tacgtgcgcc aataagaaat
    18961 tctggggaaa aaccggcatc gaaaagccaa gatccaaaaa aaagtaaagg agctatacca
    19021 cttccaccac ctatcagcaa aattttttca tctttttttt cgggaaaaga aaacgaattt
    19081 ccaagcggta aaatcatatt tacagaggct cctggagaag tttcagacag ttttaatgtt
    19141 ccggctccaa cgacttgaat cagcagccac aactcattat tttcgtaatc tacaaaattt
    19201 acggagaaag ggcgtcggag aaaagtagcc ggcgaatcat taattttaac ctgagcaaat
    19261 tggccgggca aaatttcggg aagtcctttt tcagaagaaa aaatcaacaa ataaacttgt
    19321 tcgtttaagc gaatattttt cttcaaaatt aaatcgagaa tcaatttttt catttagaat
    19381 aagtaaagca gaatgttata acattaaacc taaaaactcg gtcaatgata atgcaaaatt
    19441 aaaagaaaaa attaatccgt gaaaaacatc aacttttctc aagtagtagt aaattcctga
    19501 aaagtattgt cattaaaata cacaataatt cttttaatga ttttttcaac cactacagga
    19561 gcaattttat tctcttcaga aattttactg tttaccgact cggatataat ttccttctgc
    19621 tcgggttttt gctggaagtg cgattgaagt gattcaattt tttgctctgt tttttgagta
    19681 taatttactg tatttgtgtt agataaatct tgcgaatcga ataaagtagg cgttttagat
    19741 tgccgttctg tacgactcat tggcccttca ccgaaaataa gccagtcaga attaatgtaa
    19801 tcgaatcgtt tcaaaatttt catcaccact tccaagcttg gtttgttgcg tccattaagg
    19861 atgtgcgaca aagtggaacc ttgtattcca atttcggatg caaactgaac agcattcata
    19921 gcttcatttt gcataatttt ttcaattctg tcttttagat ccatgattca ataaaatttt
    19981 aaagagaatt gatgagattg attgaaatta caaagataaa tcattaattt cacaaaaaca
    20041 aatttcatta caaaagtaat atcaattttg taaattacaa aagtgaattt atcaatttac
    20101 aaaaatgaaa aattaacgta taaaaatcag tcgtatttaa taaggctaca atacattaaa
    20161 atgatttatt aatggaatag tttaagttat atcgaaaaac aaagttatat attataaata
    20221 attggtttac aattaaatgg attatgaaaa catgtaaatt tcgatattat gacagaatat
    20281 atttatggaa gtaaaaattt tattaaagtc aaaatataga tattataata taattgtctg
    20341 gtttaatgta aattattagt aatttttttg tttaaaataa tttttacaaa tatgaatgta
    20401 tagaattgtt ttatatgaat tattgagttt actaaagtga aaatatttat ttaaatcaag
    20461 caattacatt tgtaaatagg cgaaatatat ttgtgaaata tacaaatgta tagattcctc
    20521 aagtcgaaaa aagatgatat aaaaatttct ttgctatgat ctactcaagg agggatatag
    20581 acactttgaa tgaataatga aaaactaaaa gaacaatgta aaaattaaga aagagagggg
    20641 aagaaaataa gataatatag ctcaaggctc ctattcttga actttgaaat aaaattgtca
    20701 ttcttttcag ttagaaaaat acaatttaaa aatatcattt agagcaatat acaaacattt
    20761 ttgttctctg agatttcaat atttgtactt ttccttatgt ggaataagaa ataatggaat
    20821 ttcagcacat aaaaaagcct gaatgaacta catcactcag gcaattaaaa aatttcaaaa
    20881 aatatttttt gcttttagct ttctgaagtc ggaggaacaa ttccggccaa ttcgggatcg
    20941 accagaatgc ggccacaata ttcacacact ataatttttt tgcgcgagcg aatatccaac
    21001 tgtctttgtg caggaatttt attgaagcat cctccacaag catcgcgctg aacgggaaca
    21061 accgaaagac cgtttctcga atttttacga atgcgtttga atgccgtcaa aagtcgaggt
    21121 tcaactcgtt gttcaatttg ttttatttgt tctctcaatc tctcctcttc ctgtttggtt
    21181 tcagaaataa tttcattcaa ttctcctttt ttttggtcca aatccaagcg tcgttcggat
    21241 aaggcttcca ttgtgctttg ctgttctgcc tttttagctt cgagcgctgc agtgtattct
    21301 ttgatacgtt tttcgcacaa ctctatttcg agcgtctgat attcaatttc tttggaaaga
    21361 ttgtcatatt cacgattatt tctgacattt tcctgttgct ccttgtactt ttctatttta
    21421 gtacgagctt gctctatttc caattttttg gctttgatgg cagtttccag ttcggcaatt
    21481 tcatttttat aattttccag tcgggtattc aaaccaatga tttcatcttc aagatcttgt
    21541 acttccaaag gaagttcatc tctcaaattt ctgatttcat caatttttga atacactctc
    21601 tgtagttcat ataatgccct gagcttttct tcaacgctaa tttctttttc tgtttttggt
    21661 tctgtagcca tattgattac aaataattaa tgggatttgt ataggtttct gagattttga
    21721 caacaaagtt aggaattttt ttcgagataa tttcataaaa aatatctttc gtaaattgtt
    21781 cagattcata atgtccaaca tctgcaatca aaatttttcc ttcagcatta aaaaattcat
    21841 gatatttaaa gtccgcagaa atataaatat cggctccctg ttgaacggca tctttcaa
//
LOCUS       DAOSCH010000021        21671 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1721_length_21671_cov_5.897900, whole genome shotgun sequence.
ACCESSION   DAOSCH010000021 DAOSCH010000000
VERSION     DAOSCH010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21671
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1721_length_21671_cov_5.897900"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..737)
                     /locus_tag="PLZ54_03000"
     CDS             complement(<1..737)
                     /locus_tag="PLZ54_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOJ65937.1"
                     /translation="MIRKFFVLFFGFNIFIDSLLWAIDPVTALPIPKQVVFANETVSL
                     ERIDMRERYDREQTVIAYNHSTSILLLKRANRFFPVIEPILKNNGIPDDFKYLAVVES
                     NLDLRAFSPAQAAGIWQLMPKTAEQYGLEVNDQIDERYYLEKATVAACQFLKSAYAKY
                     HSWVTVAASYNAGMAKISGEQEKQQVDDAYDMLLSTESSRYIFRILAMKQFFENPKLF
                     GYQLKKDDFYPYIPTKEIVVSTSVEDWT"
     gene            1096..1548
                     /gene="coaD"
                     /locus_tag="PLZ54_03005"
     CDS             1096..1548
                     /gene="coaD"
                     /locus_tag="PLZ54_03005"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOJ65938.1"
                     /translation="MKRAIFPGTFDPFTIGHYSIVKRGLKLFDEIIIGIGVNQAKKTL
                     FSIEKRQDIISQTFKSEPRVKVSIYDSLTVDFARSVDAGFILRGLRSVSDFEYEHNIA
                     DANRKISGIETVILFTDYEYSYISSTVVRDLIVYGKDISIFLPPNVKI"
     gene            1758..3410
                     /locus_tag="PLZ54_03010"
     CDS             1758..3410
                     /locus_tag="PLZ54_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ65939.1"
                     /translation="MKKKKFFLFLLIFCSPFFLVAQQDNQRTFRISKNLNIYNSLLRE
                     LDMFYVDTLNYDKLIKTSIDEMLNTLDPYTIYLPEEETEDLTFMTTGEYAGIGALIMK
                     NGNEIVVSEVYEGMPAQRNGIRPGDIIVEVDGKKTDGFAVNDVSNLLRGTPNSQINIK
                     IKRYGEKKIISKTFLREKIQINPIGYAAEVDDKTGYLLLNEFTENAALGVKSAVNDLI
                     KNHHIESLVLDLRNNGGGLVDEAVQILGYFLPKGTTVVTTKGKNNILDRIYKTPTEPV
                     FPNLKLAILVNRSSASASEILAGAIQDLDRGVIVGERTFGKGLVQNIRNISYGGHLKV
                     TTAKYYIPSGRCIQAIDYSHRNEDGSVGYVPDSLTSEFLTKNGRKVRDGGGIIPDKQT
                     ESKGKLNVAHYIFAQNLYFDFATRYASLHPTIASPKDFKLSEDDFNNFVDYLHEKNFT
                     YSYQTNKYFQQLYQVAKIEGLDSIAQSEFEALKTKLTPNVDADIRENKDEIEALLSLE
                     IIKRYYYQKGQIEFSLRTDDDLKEAIQILKSEETYRKILSAI"
     gene            3527..4123
                     /locus_tag="PLZ54_03015"
     CDS             3527..4123
                     /locus_tag="PLZ54_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOJ65940.1"
                     /translation="MAVITPFIMEKKNIGEKILALCKSRNLSIQDLVDTTGLSEVQIN
                     RVIESETIPSLAPLIKIARALGVRLGTFLDDAEELGPVVHRSGEVQQPASFSSQLSDA
                     NSHLDFYALASRKTNRHMEPFFIDIQPSSTHEPIFSSHEGEEFIFVLEGAAKIDYGNQ
                     TFFLHPGDSIYYDSIINHLVSSADEKPAKILAVVYTPL"
     gene            4227..5876
                     /locus_tag="PLZ54_03020"
     CDS             4227..5876
                     /locus_tag="PLZ54_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ65941.1"
                     /translation="MELFEKTIGEWLEYWANETPEREYLVYADRNLRFTWKSFNERVD
                     NMAKGLMAIDVKKGDHVGIFAQNVPDWLTFLYACAKIGAIAVTLNTSCKEHELAYIIE
                     NSDMHTLCMTEGDNGNDFIKTIYSLIPELKTSERGRLKSNRFPVLKNVVFIGQEKHRG
                     MYTTAEILLLGTYQNNEEYFQLKKTINCHDVVNMQYTSGTTGFPKGVMLTHHNITNNG
                     FYTGEHMKFTNDDKLCICVPLFHCFGIVLAVMNCLTHGCTQVMVEKFDALLTLASVHK
                     ERCTALYGVPTMFIAELNHPMFNLFDLTSLRTGIMAGALCPIELMRNVNEKMYMNITS
                     VYGLTETSPGMTQTRVDDSFEVRCTTVGREYEFVEVAVLDPKTGAPCPDGVEGEMCCK
                     GYNVMKGYYKNPEATHQVIDANGFLHSGDLGVRDKNGNYRITGRIKDIIIRGGENISP
                     IEVEDFLYQMPGIKDVQVVAIASPRYGEDVAAFIIKKDGFDIREEDVRDFCKDKIARY
                     KIPRYVFFVDEYPLTGSGKIQKFKLREMALQICKERGIEIV"
     gene            5935..6714
                     /locus_tag="PLZ54_03025"
     CDS             5935..6714
                     /locus_tag="PLZ54_03025"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOJ65942.1"
                     /translation="MKKLWRIYRYFLHRFTARNTHGFGVHSPFLFHFICFVVEEKNPY
                     YIFSSIENLRDRLLKDRTKIQIEDFGTGKSRTSTIATVVNKSVKSKKYGQLFFRITLH
                     TKAQSVLELGTSLGITTAYLAAASSQLHCISLEGSQTLAALAKKNFQQLGLKNISLIE
                     GNIDENLEKVLESTPSFDLIFIDANHTSQAVIEYFNMCLEKIHTHSVMLVDDIYWSAD
                     MLKAWKYIKQHPKVTATIDLFETGIVFFDPNLYKKHYKVRY"
     gene            6864..7424
                     /locus_tag="PLZ54_03030"
     CDS             6864..7424
                     /locus_tag="PLZ54_03030"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOJ65943.1"
                     /translation="MKLYTKTGDEGETGLIGGTRVPKNHIRLEAYGTVDELNSFIGLL
                     TTFQIDNSDLLFLQSIQNKLFTIGSYLATDFSKFSKTKVSPLFEKDITSIEKEIDRLT
                     SEVPALKNFVLPGGSQASALCHICRTITRRLERRLYDLHEQIPVDKNILIYINRMSDY
                     FFALSRYLLFKNNIQEILWQKNEEND"
     gene            7512..7733
                     /locus_tag="PLZ54_03035"
     CDS             7512..7733
                     /locus_tag="PLZ54_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOJ65944.1"
                     /translation="MYWTLELASKIEDAPWPATKDELIDYAIRSGAPLEVIENLQEIE
                     DEGEIYESIEDIWPDYPSKEDFFFNEEEY"
     gene            7916..8863
                     /locus_tag="PLZ54_03040"
     CDS             7916..8863
                     /locus_tag="PLZ54_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HOJ65945.1"
                     /translation="MKKIVLTVVVFMVSSVSIISQTASEWSQYMFNSSSLNPAAVGEK
                     GMIEAVWQHRIQWVGMPNAGQSDIINLNLPVDFLSTTHGVGLKVIDDKVGLFSNTSVY
                     LQYAYKLKTTSGVWSFGTELGLINAGFEGDSVHSVNIGDYHESTDPVVPSTNVQGMGF
                     DIGLGAWYSSPKFEAGISYAHLNQPVISWSDQLDYQEKGVLFITGGYNWTLIDPKMVI
                     KPTTLFKTDFVSYQWDASARMEYDNKYWGGLSYRWGDAVVFFAGINAIAGLNIGYAYD
                     LPVSQIIKASSGSHEFLISYSFEYKLNKAKHKYKSIRIL"
     gene            8933..10288
                     /locus_tag="PLZ54_03045"
     CDS             8933..10288
                     /locus_tag="PLZ54_03045"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOJ65946.1"
                     /translation="MLISILFGSLLLAGCNKPAGELIGVGNRGNFREANPYGMVFIKG
                     GSFLMGANDQSAIGAINDKSIHVTVDAFWMDQTEITNDEYKQFVNWVRDSIILRSLVM
                     AGRDEFRLKLRNQDELPPETAPLNWKAKIPWKSQDEEIREVLNTFYYEDVNSLSGRQL
                     NPTKFRYRYEWINYDQAALASNKYDVNTGAYPKNAKVRVDTSYVDENGVIHHQTIERP
                     LRTRKDLISTKIVNIYPDTIMWIRDFQYAYNEPKMKMYFSHPGFANYPVVGVTWEQAQ
                     AFCQWRTHIYNGTNKISAQDYRLPTEAEWEYAARGGRQMALYPWGGNYVRDKRGCFMA
                     NFKPMRGSYNDDTGTTTMKVGSFLPNDFGLYDMAGNVAEWTSSAFNVSSSNIVYDINP
                     NFQFNAKNSDPQVLKRKVVKGGSWKDIAYYLQCGARTYEYQNESRPYIGFRCVRSYNG
                     E"
     gene            10315..11193
                     /gene="gldL"
                     /locus_tag="PLZ54_03050"
     CDS             10315..11193
                     /gene="gldL"
                     /locus_tag="PLZ54_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOJ65947.1"
                     /translation="MSNNAINSKKQSGFSEWWNSPKGRKIVNAIYSVGAGVVIIGAMF
                     KILHLPGANVVLGTGMICEAIIFSMGALDKPFKEYHWDKIFDFENGTARFQTELIGSA
                     TISASPAHPSINYSETLSEEDVEKLSEGIKNLSATARQLASVSDLTAPTSEFIRNITS
                     ASESVSNFSKDQNNLNSSLRNLSGIYVEIGTEMKKAEENTKDYTGEIEKINKNLSSLN
                     AIYEIHLKNVEEQSTKIGNSNKELENILSNLKKINSFSSASAEESEKYKNETVKLAQQ
                     ISDLNKVYGNMLNALS"
     gene            11256..12791
                     /gene="gldM"
                     /locus_tag="PLZ54_03055"
     CDS             11256..12791
                     /gene="gldM"
                     /locus_tag="PLZ54_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOJ65948.1"
                     /translation="MSGAKNCPETTRQKMIGMMYLVLTAMLALNVSSEVLEGFTMVDN
                     SLHNTIISASVRNEDLYKQFEYLYNQNPTKVKEWLDKALDVKKQSDSLYNYIQNFKYE
                     ILKMVDNKNADPEARRVINKENYDAPGEYALVKGNGKILKEKIDNYRNFLISLSGNSP
                     AKQQMYATIFSTAPSENGVPWEYALFEMMPVSAVITVLTKYQSDIRMAEAELIQYLKA
                     QTDVSDFRVNKIEALVVPNSRYVFRGSKYSAQIVLSAVDSTKTPEFYVGNTPVKNGIY
                     EVNCNQTGSFTYTGFVQLIGNDGVIRKFPFTSDYIVGEPSATISNEDLNVVYRGIDHK
                     FSISVPGVASENISVRVAGATVQKTAGGKFIIRPTQDGEINVAVYAKVEGKEILMGNS
                     IYRVRYLPDPKAYLQYSDAGGITRLIQDGNLNKRLLRGDNVAIVASYGEDELIQAKFN
                     IISFTMLTIYGSVETTGSRLSNRQLADIDRLEGGDYITFKNIKAVGPDGKVRNLGLVQ
                     VQI"
     gene            12841..13743
                     /gene="gldN"
                     /locus_tag="PLZ54_03060"
     CDS             12841..13743
                     /gene="gldN"
                     /locus_tag="PLZ54_03060"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOJ65949.1"
                     /translation="MKKIGVLTLFLAFILLPGSIKAQINEIPFFDSKGIVNIETTELD
                     TLADTIAVVHHRRDDIVWSRVVYRVIDMREKQNYQLYFPTRPNKEYRNLFTVMIDAIC
                     DGVNVYKKNPRDIKPEFNELLEGEELSRAFAFEEDNTSNLIQIDPITNQRTFNNYRYI
                     QYVRNQLKYLIQEIIFFDKHTSRMYSKIIGIAPLYALHPDNVESNETMGYFRGSVLCW
                     FAFDELRPYLAKQYVIPNGNDTQRMTFDDFFSQKLYASYLLGDSNMFNRMLLDYVIDP
                     AKVRKEQNRIENELLNFEQDLWEY"
     gene            13950..14600
                     /locus_tag="PLZ54_03065"
     CDS             13950..14600
                     /locus_tag="PLZ54_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ65950.1"
                     /translation="MKNIILSDNQYISKAGWLYLISTYSLEATIREAATKKELEKLLV
                     EEQESLVILDYTLFDFENVNELIIWSMRFEKTEWLLFSEELSEEFLQQIIYNTHSFSV
                     LTKNSNKEEINLALKETLSGNRFICNFVSNLLIDCQKHLKNDGKPVLTPTEREILKEI
                     ALGKTTKEIASERFVSVHTIMTHRKNIFRKIKVNNVHEATKYAMRAGIVNLAEYYI"
     gene            14615..15676
                     /gene="mnmA"
                     /locus_tag="PLZ54_03070"
     CDS             14615..15676
                     /gene="mnmA"
                     /locus_tag="PLZ54_03070"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOJ65951.1"
                     /translation="MKIAALLSGGVDSSVVVHLLKEQGYTPSLFYIKIGRDEDEMLNC
                     SSEEDIEMASLVAQKYDCKLEIIDLHKDYWETVVAYTISKVKEGLTPNPDVMCNKLIK
                     FGIFEQKVGKEFDKTATGHYATITEKDGKIFLSTAKDPIKDQTDFLAQIDYLQVSKLM
                     FPIGHFMKSEVRSIAQKAGLPSAKRQDSQGICFLGKINYNDFIRRYLGEKPGPIVEWE
                     TGKILGKHKGYWFHTIGQRKGLGLSGGPWYVIKKDIAANIVYVSCGYDVETQYGNEFS
                     MRNFHFITENPWQNQTEEANITFKIRHTPEFTKGKILHTNSGWHVISEKKIQGIAPGQ
                     FGVIYDKDSHICIGSGEIF"
     gene            15742..16968
                     /locus_tag="PLZ54_03075"
     CDS             15742..16968
                     /locus_tag="PLZ54_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOJ65952.1"
                     /translation="MTYQTHTLPNGLRIIYLQKKSEVTFCGITINAGSRDENREEEGC
                     AHFAEHLLFKGTRKKNAREIINRMEDIGGELNAYTSKEETVVYAAAMKKYTRRMIELM
                     ADMTLHSTFPQKELEKEREVILDEIASYHDNPSELIFDDFEELIFKDHPLGHNILGNA
                     KLLKKMNSESVSNFVKRCYQPHQMIVFVVGNLNFEHFVKWCEEYFVSESAENINEKQK
                     RNPPEQYRAEKLKLKKKTHQIHTVIGNRCFDLYHPNRLPMYLLNNILGGPGMNSLLNL
                     SLREKHGLVYTAESNYQPFTDSGWWCVYYACDERNDEKCEQLVLEQLQKIVDRPLPES
                     KLRKYKQQLIGQMTVSRENTENFALSLGKSYLRFGKVEEMEEIKKQLENIQPKDLQYI
                     AQEIFNEEQLSVLKYY"
     gene            complement(17124..18107)
                     /locus_tag="PLZ54_03080"
     CDS             complement(17124..18107)
                     /locus_tag="PLZ54_03080"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GSCFA domain-containing protein"
                     /protein_id="HOJ65953.1"
                     /translation="MTNYLFRTPVELAPSQQKITYKDKIMMLGSCFSENIGKQLHEAF
                     FQVDVNPFGVLYNPLSISLSLERLIENKGFSRSDIFECQCLWHSFFHSSSFSDVSPEK
                     TLSNIQARFDESCSFLKNTDFLLLTFGTAWVFEDRKTQTVVSNCHKLPSQHFNRRRLS
                     IEEIVEHYTLLIAQLQQLNPSLHLIFTVSPIRHWKDGAHENNLSKSILLLSIEELQKK
                     FKNVDYFPAYEIQMDELRDYRFYASDMLHPSETAINYIWEKFSDTYFSEKTKQIKQRL
                     EMLREKMNHRPLHPNSPEYVSFLQQIELEKNNILNEYPFLKDRIKDVNSRN"
     gene            18521..19087
                     /locus_tag="PLZ54_03085"
     CDS             18521..19087
                     /locus_tag="PLZ54_03085"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HOJ65954.1"
                     /translation="MPLDSVIPAFVREQSSDYRNNNLFMLNTLNLQNYFAFNNDHILE
                     GFSLWSDENIFSYGIKRYVFMGGAHGLSTQTFLNFDLKTGKTLTEKDLFIPDYKEKLT
                     TLIRERLIEENDEISEYRDLNQTDYWVDSIQPNGNFYISDEGINYLFNPYEIGPYYLG
                     ETEVRIPYARLKDILKNGNPLEYLYENR"
     gene            19099..19626
                     /locus_tag="PLZ54_03090"
     CDS             19099..19626
                     /locus_tag="PLZ54_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOJ65955.1"
                     /translation="MNCFFNLAKTRRSIRKFLPQKVEPEKISMITKVALMAPASKRSN
                     PWEFVVVQQPEMLQKLSQCRPKNSQFLAGAPLAIVVLADTEKSNVWMVDAAIASIMMQ
                     LQAHDLGLGSCWIHVHDRQMDENTSSEDYIRGLLQIPTQYAILNILAVGYPNETKNPH
                     DEEKLAYEKVHFEKF"
     gene            19642..19965
                     /locus_tag="PLZ54_03095"
     CDS             19642..19965
                     /locus_tag="PLZ54_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65956.1"
                     /translation="MASKKKSSNLAWGITLLLFGLLFLLRQLQIVPPEIGVFVFDIKN
                     FPFIAGVIFLLFHPNISIGIVLLAVGVLFRLSEIIAWTKNFSEFILPALLIIAGSILI
                     FSKRK"
     gene            20051..20845
                     /locus_tag="PLZ54_03100"
     CDS             20051..20845
                     /locus_tag="PLZ54_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009434040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOJ65957.1"
                     /translation="MKRVVIIGAGNVATHLGRALQNKNFVVSQIFSRTENSATQLASD
                     LGVPFTTNLQSLDREADIYFYCVNDAVLCDVINKIDIPDALHVHTAGSLPLSIFESHL
                     QYRRYGVFYPLQTFSKTRKVNFDEIPIFVEGNSLEAEEEIRQIAQTLSKRVFSSDSEQ
                     RKKIHLSAVFACNFVNHLFSIAEELVKTAGYSFDILLPLITETVNKLSELSPYDAQTG
                     PAVRNDQNIIRQHLLLLENKPEFSEIYSVLSESIYRMHKKYIQTRE"
     gene            20905..>21671
                     /locus_tag="PLZ54_03105"
     CDS             20905..>21671
                     /locus_tag="PLZ54_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOJ65958.1"
                     /translation="MKQYLQLLRLPNLLFIAFVQFAMSEAVILPLLMTYGFDAGMSSS
                     KLLLLILSTILIAAGGYVLNDYFDVKIDTINRPDKMIVGKTIPRASAMRYYQILTALG
                     LLSGFILAFLVKSFALGFIFLVVPGMLWFYSATYKRQFLTGNIIVAFASALSVLLPAI
                     LELAVLESQYGKLIFETPIPKQIYAWVGGFAVFAFFCTLLREIIKDAEDIAGDREMEC
                     HTLPIVWGIKKTKIIVYCLILLIVAGLLAADFFLIDF"
BASE COUNT         7199 a         3830 c         4190 g         6452 t
ORIGIN      
        1 gtccaatctt caacggaagt acttacaaca atttcttttg tgggaatata cggataaaaa
       61 tcatcctttt tcaattgata accaaaaagt tttggatttt caaaaaactg tttcatggcc
      121 agtattctga aaatataacg agaactttcg gtagaaagta acatatcata ggcatcatcc
      181 acctgttgtt tctcctgttc accggaaatt tttgccattc cggcattgta ggaagccgcc
      241 accgtaaccc aactatgata ttttgcatag gcgcttttta aaaactgaca ggctgccaca
      301 gtagcctttt ccaagtaata acgctcatca atttgatcgt tgacttccaa cccgtactgt
      361 tcggcggtct tcggcattaa ttgccaaata ccggcagcct gagccggaga aaaagcacgc
      421 agatcgagat tgctctctac aacagccaaa tatttaaaat catccggaat accgttgttt
      481 tttaaaatag gttcgattac ggggaaaaac cgattggcac gcttcagcaa taaaatgctg
      541 gtggaatggt tataagctat caccgtctgt tctctatcgt aacgttcacg catatcgatt
      601 ctttccaaag aaacagtttc attggcaaaa acaacctgtt tgggtattgg cagtgccgta
      661 accggatcaa tagcccacaa aagggaatca ataaaaatat taaaaccaaa aaacaaaaca
      721 aaaaattttc gtatcatcac aaaatctttc aattaattgc agcaaagtta ataaaccatt
      781 ttaaaactga aaataaaatg caaaaaaatg cacttcgatg cagaatttag ttggccataa
      841 aagaattgaa aaaataaaac ctgaaaccaa tgaacgacgt ctgaaaattc tgtaagggga
      901 actcgagaaa aaccaatttt atcagcgata tacagaaatt gaaaattatc atgtaaattt
      961 gcacataaat aaatttaatc aaaaatgaat ggacatccct tgttgcaaaa aatggtttcc
     1021 ttagtagcaa aaaagtgcga aatgagggca actttccttc aaccaagttc ttgtcgattc
     1081 aaaataattt agatcatgaa acgagccatt ttcccgggaa cgtttgaccc atttaccatt
     1141 ggacactaca gcattgtcaa gcgaggattg aagcttttcg atgagataat tattggcatt
     1201 ggagtcaatc aggcaaaaaa aaccttattc tccatcgaaa agaggcagga cataattagt
     1261 caaacattta aaagtgagcc aagagtgaag gtttctattt acgacagcct cacagtggat
     1321 tttgcccgtt cagtcgatgc aggtttcata cttcggggat tgcgctctgt tagcgatttt
     1381 gaatatgaac acaatattgc cgacgccaac cgaaaaattt ccggaattga gacagtcatt
     1441 ctgtttaccg attacgaata ttcgtatatt tcttcaactg tggtgcgaga tttaattgtt
     1501 tacggaaaag acatttcgat ttttctgccg cctaatgtaa aaatttaata aattcatttt
     1561 cgatcattta tattcattca aacgaaaaga aataaaaata atttccctaa gaattttgtt
     1621 ttcctacaaa atccattcaa gaaaaaaatt aatgacaaac gcatgaaacc cacatgtatt
     1681 gcatacacga acacgaataa atataatatt cagacatgtg ggttccatca gacctttaaa
     1741 aaataattta tattctgatg aaaaagaaaa aattcttcct gttcctccta attttttgct
     1801 ctccattttt tcttgtggct cagcaagata atcaacgtac atttcgtatc agcaaaaatc
     1861 tgaatattta caattcattg ctgcgtgagt tggacatgtt ttatgtcgat accctgaatt
     1921 acgacaaact gataaaaact tctatcgacg aaatgttgaa cacactggat ccttatacaa
     1981 tttatctgcc cgaagaagaa accgaagacc ttacctttat gacgacaggc gaatatgccg
     2041 gtattggtgc acttatcatg aaaaacggaa atgaaattgt tgtttccgaa gtttatgaag
     2101 gcatgccggc gcaacgtaac ggtattcgac ccggggacat cattgtggaa gtagatggga
     2161 aaaaaaccga tggctttgcc gtcaacgacg ttagcaattt attgagaggt actcccaata
     2221 gccaaatcaa cataaaaatc aaacggtacg gagagaaaaa aatcatcagc aaaacttttc
     2281 tgcgtgaaaa gattcaaatc aatcccattg gctatgcggc agaagttgat gacaaaacag
     2341 gctatctgct gctcaacgaa ttcaccgaaa acgcagcgtt gggagtaaaa tcggctgtaa
     2401 acgatttgat aaaaaatcat catattgaat ctcttgtact cgatttacgc aacaacggag
     2461 gcggattggt ggacgaagcc gttcaaattt tgggatattt tttgcccaaa ggcacaacag
     2521 tggttaccac caaaggaaaa aacaatatac tcgacagaat ttataaaact cccaccgaac
     2581 ctgtttttcc caacctcaag ctggcaattt tggtcaaccg atcttcagcc tctgcctccg
     2641 aaatactggc cggagccatt caggatctcg atcgcggtgt aatagtcgga gaacgtacat
     2701 ttggcaaagg attggttcaa aacattcgca acatcagtta cggcggacat ttgaaagtta
     2761 cgacagcaaa atattacatt ccgagtggca gatgcataca agccatcgac tacagtcatc
     2821 gcaatgaaga tggaagtgtg ggatacgtac ccgacagcct gacttccgaa tttctgacaa
     2881 aaaatggtcg aaaagttcgc gacggtggcg gaattatccc cgacaaacag accgaaagta
     2941 aaggaaaatt gaacgttgcc cactatattt ttgcacaaaa cctgtatttt gattttgcga
     3001 cccgctacgc cagtttacat cctactattg cgtctccaaa ggattttaaa ctttctgaag
     3061 acgactttaa caattttgtg gattacctgc acgaaaagaa tttcacttac tcatatcaga
     3121 ccaataaata ttttcagcaa ctctatcagg ttgccaaaat agaaggctta gattcaatag
     3181 cacaatctga gtttgaagca ttaaaaacca aattaacacc caatgtagat gccgatattc
     3241 gggaaaacaa ggatgaaatt gaggcattgc tcagtctgga aataattaaa agatactatt
     3301 atcaaaaagg acaaattgag ttttcacttc gaactgatga cgatttgaaa gaagcaattc
     3361 aaattttaaa gtccgaagaa acctatcgaa aaattttatc ggccatttaa aaaaacagtt
     3421 gatattttat aagttaagta aaggtttttg attggaaata aaatattttt ccttcaaaaa
     3481 ccttcatttt ataaattttt atcgtacttt tgttcccgtt ttaaatatgg cagttataac
     3541 accttttatc atggagaaaa aaaatatcgg agaaaaaatt cttgcccttt gtaagagtag
     3601 aaatctaagc attcaagatt tggttgacac cacaggttta tctgaagttc aaattaacag
     3661 agtgatcgaa agcgaaacta ttccttcgct ggccccactt ataaaaattg cacgcgcatt
     3721 gggcgttcgg ttgggaactt ttcttgatga cgcagaagaa ttgggacctg ttgtccatcg
     3781 ttccggcgaa gttcagcaac cggcttcttt ttccagtcag ctttcggatg ccaattctca
     3841 tctcgatttt tatgcgttgg catcacgcaa aaccaaccga cacatggaac ccttcttcat
     3901 cgatattcag ccttcatcga cacacgaacc cattttttct tcccacgaag gagaagaatt
     3961 tatttttgtt ctggaaggag cagcaaaaat agattatgga aaccaaactt ttttcctcca
     4021 tcccggcgac agcatttact acgactctat tatcaaccat ctcgtcagct cggctgatga
     4081 aaaaccggcc aaaattctgg ctgtcgttta cacacctctt taatcttttc accttgttga
     4141 attattgaga attttattgc tgttttttcc ggtctgaaaa aattttattt ccatcaagca
     4201 ccataaagct ttatacaaat cttatcatgg aattatttga aaaaaccata ggcgaatggc
     4261 tcgaatattg ggcaaatgaa actcccgagc gtgaatacct tgtttatgcc gatcgaaatt
     4321 tgcgtttcac atggaaaagt tttaacgaac gtgtggacaa tatggccaaa ggtttaatgg
     4381 ccatcgatgt aaaaaaaggg gatcatgtcg gtattttcgc tcaaaacgtg cccgactggt
     4441 tgacttttct ttatgcctgc gccaaaattg gagcaatagc cgtaacactc aataccagtt
     4501 gcaaagagca cgaacttgcc tatattattg agaattcgga catgcataca ttgtgtatga
     4561 cagaaggcga caacggaaat gactttatta aaaccatcta ttcgttgatt cctgagctaa
     4621 aaacttcgga acgcggaaga ctgaaatcaa accgctttcc tgttttgaaa aatgttgtct
     4681 ttatcggaca ggaaaaacac aggggaatgt atactactgc tgaaattctg cttcttggaa
     4741 cttatcagaa taatgaagaa tattttcaac tgaaaaaaac gatcaattgc catgatgtgg
     4801 tcaatatgca atatacttcc ggaactaccg gtttcccaaa aggagtaatg cttacccatc
     4861 acaacataac caacaacggg ttttacacag gagaacacat gaaatttacc aatgacgaca
     4921 aactgtgtat ctgtgttcct cttttccact gttttggcat tgtattggca gtaatgaatt
     4981 gtctgacaca tggttgtaca caagtaatgg ttgaaaaatt cgatgcactt ttaacgctgg
     5041 cttctgttca taaagaacgt tgcacggcac tttacggagt ccccactatg tttatcgccg
     5101 agctgaatca tcccatgttt aatttgtttg acctcacctc attgcgtacc ggcatcatgg
     5161 caggtgcact ctgtcccata gaactgatgc gcaacgtaaa tgaaaaaatg tatatgaaca
     5221 tcaccagcgt ttacggactc accgagacat ctcccggtat gacgcaaaca cgagttgatg
     5281 attcgtttga agttcgctgt accaccgttg gaagagaata tgaatttgtt gaagtggcag
     5341 ttctcgatcc aaaaacaggc gctccctgcc ccgatggtgt agaaggcgaa atgtgttgca
     5401 agggctacaa cgtaatgaaa ggttactaca agaatccgga agccacccat caggtaattg
     5461 atgccaatgg atttttgcat tcaggtgatt tgggagtcag ggacaagaac ggaaattatc
     5521 gaattacggg acgaattaag gatattatta ttcgaggtgg cgaaaatatt tctcccatcg
     5581 aagttgaaga ttttctttat caaatgcccg gaataaagga cgttcaggtc gtggccattg
     5641 cttccccacg ttacggagaa gatgttgcag cttttattat caaaaaggat ggatttgaca
     5701 ttcgggaaga agatgtcaga gatttttgca aagataaaat tgcccgttac aaaattccgc
     5761 gttatgtatt ttttgttgat gaatatccat tgaccggcag cggaaaaata caaaaattca
     5821 aactgcgcga aatggctttg caaatctgca aggaaagagg aattgaaatt gtttgaatgt
     5881 aacggtgaaa ataaaaatgt acctttgtaa ggtttcttga tatttttttt cgcaatgaaa
     5941 aaactatggc gtatttacag atatttttta catcgtttta cagcaagaaa cacgcatggt
     6001 tttggcgtac attctccatt ccttttccat tttatttgtt ttgttgttga agaaaaaaat
     6061 ccctattata ttttttcttc catcgaaaat ttgagagatc gcctcctcaa agacagaaca
     6121 aaaatacaaa ttgaagattt cggtacgggt aaaagcagga cttcaaccat tgcaacggta
     6181 gtcaacaaat cggtaaaatc aaaaaaatac ggtcaacttt tttttcggat aacactccac
     6241 accaaagcgc aatctgttct tgaactggga acttcactcg gtattacaac ggcatatctg
     6301 gctgcagctt cttctcagct tcattgcata agtctggaag ggtcccaaac attggccgcc
     6361 ttggcaaaaa aaaatttcca acaattggga ctcaagaata tttccttaat cgaagggaat
     6421 attgatgaaa acttggagaa agttcttgaa agtactccgt cattcgactt aatattcatt
     6481 gatgccaatc atacgtcgca agccgtaatt gaatatttca acatgtgtct tgaaaaaatt
     6541 cacactcatt ccgtaatgct ggttgacgat atttattggt ctgccgatat gctgaaagcc
     6601 tggaaataca tcaaacaaca tccaaaagta acagctacca tcgatttgtt tgagacagga
     6661 atcgtatttt tcgaccccaa tctatacaaa aaacactata aagtgcgtta ctaaattgct
     6721 attgcacaaa acatattaat gtatttgttc aacaaaaaat cacgaaaaat tgttatgtgg
     6781 tcagattgct attgaaaaac aaatcaacaa attaaaaaac aactgatttt caaaacaata
     6841 gaaaaataaa gaaaccaaac agtatgaaac tatatacaaa aacaggcgat gaaggtgaaa
     6901 cgggattaat tggaggaacg agagttccaa aaaatcacat tcggctggaa gcttatggta
     6961 cggtagatga gctaaattca tttattggct tgttgaccac ttttcagata gataattcgg
     7021 atttactttt tctgcaatcc attcaaaata aactttttac cattggctct tatttggcaa
     7081 ccgatttttc taaattttcc aaaactaaag tttctccact ttttgaaaaa gacatcacaa
     7141 gcatagaaaa ggaaatagac cgattgacgt cggaagttcc ggctttgaaa aattttgtct
     7201 tgcccggtgg cagtcaagcc tcagccttgt gccatatctg cagaaccatt acacgtcgtt
     7261 tggaacgacg tttgtatgac ttacatgaac aaatacctgt cgacaaaaat atactgattt
     7321 atatcaatcg catgtccgat tatttctttg ccttgtcgcg atatttgttg tttaaaaaca
     7381 atattcagga aatattatgg caaaaaaatg aggaaaacga ttaatttgta agttttttaa
     7441 ttatttttgc gcaaaattta gaggaaaaat aaatattcaa tcaaaaaaat tgaacctaaa
     7501 tatttagcta tatgtattgg acgttagaat tagcttcaaa aatagaagat gcaccctggc
     7561 cggcaactaa ggatgaattg atagattatg ctatccgctc cggtgcgcca ttggaagtaa
     7621 tcgaaaactt gcaggaaatt gaagatgaag gtgaaatcta tgaatccatc gaggatattt
     7681 ggcccgatta tccgagtaaa gaagactttt tcttcaatga agaagaatat taatctttca
     7741 aaattctttt tattacaaaa ctttacttat ttttgttgag ctaaaagtaa ggattaggag
     7801 taatggatac ggtgaaagtg caattttttt gttaaattga agttatttat aaagttgaca
     7861 agcttttttg atcgaaaaaa aggttgaata aaaacttaat aataagaatc atctcatgaa
     7921 aaaaatagta ctcactgttg ttgtatttat ggtttcatca gtgagtatta tttctcaaac
     7981 tgcatcagag tggagccagt acatgtttaa ttcttcttcc ttaaatccgg cagcggtcgg
     8041 cgaaaaagga atgattgaag ctgtttggca acatcgtatt cagtgggtag gaatgcccaa
     8101 tgcgggtcaa tcggatatta ttaacctcaa cttgcctgtt gattttttgt ccaccactca
     8161 tggtgtcggt ttaaaagtca tagatgataa ggtaggctta ttttctaaca cttcagtata
     8221 tctgcaatat gcatataaac taaaaacaac atcaggagtt tggagttttg gaacagaatt
     8281 gggtttgatc aatgcagggt ttgagggaga cagcgttcat tcagtcaaca taggtgatta
     8341 ccacgaatcg accgatcctg tggtaccatc aaccaatgtt caagggatgg gtttcgatat
     8401 tgggttggga gcttggtact cttcgccaaa atttgaggca ggaatttcct acgcacatct
     8461 caatcagcct gtcattagtt ggagcgacca acttgattat caggagaaag gtgttctttt
     8521 tatcacaggt ggttacaatt ggactttgat tgatccgaaa atggttataa aaccgacaac
     8581 gctctttaaa actgattttg tttcttatca gtgggatgct tctgcacgta tggaatatga
     8641 caataaatac tggggaggac tttcctaccg atggggcgat gcggtggtat tttttgctgg
     8701 aatcaatgcc attgctggtt taaatattgg ttatgcttat gatttaccgg tttcgcaaat
     8761 tataaaggca agttccggat ctcatgaatt tttgataagt tatagttttg aatataaatt
     8821 aaataaagca aaacacaagt acaaaagtat tcgtattttg tgacaaaagt attttttcag
     8881 aaaaaagatt aaaaaacaat cactttattg ttatacaagg atgaaaagaa atgtgctaat
     8941 tagtatcctt ttcggatcat tgcttctggc cggatgcaac aagcctgccg gagaattgat
     9001 tggagttggg aacagaggaa attttcgcga agccaatccc tatggtatgg ttttcataaa
     9061 aggaggttcg tttttgatgg gagccaatga ccaatcagca attggagcta tcaatgacaa
     9121 gtcgatacac gtaacggtcg acgccttttg gatggatcag accgaaatta ccaatgacga
     9181 gtacaaacaa tttgtgaatt gggtgcgtga ttccatcatt ttacggtctc ttgtaatggc
     9241 tggccgagac gaatttcgcc tgaagttgag gaatcaggat gagcttccac ctgaaacggc
     9301 acctttaaat tggaaagcga aaattccgtg gaaatcccaa gatgaagaaa ttagagaggt
     9361 gctcaataca ttttattatg aggacgtaaa ctcactttca ggcagacagt tgaatccaac
     9421 caaattccgt tatcgttatg aatggattaa ttatgatcaa gctgctttgg ccagcaataa
     9481 atatgatgta aacacaggag cctatccaaa aaatgcaaaa gttagagttg acacctctta
     9541 tgttgacgaa aacggagtga ttcatcatca aactatcgaa cgtcccttga gaactcgtaa
     9601 agatttgatt tccacgaaaa tagtaaatat atatcccgat actatcatgt ggattagaga
     9661 ttttcagtat gcctacaacg agccaaaaat gaaaatgtac ttttcccatc ccggctttgc
     9721 caattatcct gtggtagggg taacatggga acaggcacag gcattttgtc aatggagaac
     9781 gcatatttac aacggtacaa acaaaatcag tgctcaggac tatcgtttgc ctaccgaagc
     9841 cgaatgggaa tatgcagctc ggggtggtcg tcaaatggct ttgtatcctt ggggtggcaa
     9901 ttatgtaaga gacaaaagag gatgttttat ggctaatttt aaacccatgc gtggaagtta
     9961 taacgatgat accggaacaa caaccatgaa agttggttca tttcttccga acgattttgg
    10021 actgtacgat atggcaggaa atgtagcaga atggacttct tcagctttca atgtctccag
    10081 cagcaacatt gtttacgata ttaacccgaa ttttcagttc aatgcaaaaa acagcgatcc
    10141 tcaagtactg aaacgcaaag tggtaaaagg cggatcatgg aaagacattg cctactacct
    10201 gcaatgcggt gcacgtacct atgaatatca gaacgaaagc agaccttata ttggatttag
    10261 atgtgtacga tcatataatg gcgaataaaa gtgtaaactt taataattac aaacatgtca
    10321 aataatgcta taaattcaaa aaaacaatca ggattttccg aatggtggaa ttctcccaaa
    10381 gggagaaaaa ttgttaatgc catatacagc gttggagccg gagtagttat catcggtgca
    10441 atgtttaaaa tactgcactt gccgggtgca aacgtggtgc taggtaccgg catgatttgt
    10501 gaggcaatta ttttttcaat gggagcatta gataaacctt ttaaagaata ccattgggat
    10561 aagattttcg attttgaaaa tggaacagcc cgatttcaaa ctgaactaat aggaagtgcg
    10621 acaatatccg cttctccggc tcatccatct ataaattatt ctgaaacgct ttctgaagaa
    10681 gatgtagaaa aattatcgga aggaataaaa aatctttctg ccacagcacg acaactagca
    10741 tctgtttctg atttaactgc cccaacttcg gagtttatcc gcaacataac ttctgcttct
    10801 gaatctgtaa gcaatttttc gaaagaccaa aataacctga actcttctct tcgtaattta
    10861 tcagggattt atgtggaaat tggtactgaa atgaaaaaag cagaagagaa cacaaaagat
    10921 tacactgggg aaattgagaa aatcaacaag aatttaagtt cattgaatgc catttacgaa
    10981 attcatttga aaaacgtgga agaacaatca actaaaatag gaaattccaa caaggaattg
    11041 gaaaatattc tatcgaactt gaaaaaaatt aattcatttt cctctgcaag tgctgaagaa
    11101 tcagaaaagt ataaaaacga aactgttaaa ctggcacaac aaatttctga tctcaacaaa
    11161 gtttatggaa atatgttgaa tgcattaagc tgataacaaa gagattttta ttttttaaat
    11221 tttcttaaaa taagacatta gaaataaaat aaaagatgag tggagcaaaa aattgtcccg
    11281 agaccacccg acaaaaaatg atcgggatga tgtatttggt gctgacagcc atgctggcat
    11341 tgaatgtatc aagtgaagtg ctggaagggt ttactatggt tgacaacagc ttgcataaca
    11401 ctatcatttc agcttcggta aggaatgaag atttatacaa acaattcgaa tatttataca
    11461 atcagaatcc cacaaaagta aaagaatggt tagataaagc tttggatgtt aaaaaacaat
    11521 cggattctct ttacaactat attcaaaatt tcaaatatga aatattgaaa atggttgata
    11581 ataaaaatgc tgatcctgaa gcgcgtaggg tgattaataa agaaaattac gatgcacccg
    11641 gtgaatatgc attggtaaaa ggtaatggga aaattcttaa ggaaaaaatt gataattata
    11701 gaaatttttt gatctcatta tctggcaata gtccggcaaa acagcaaatg tatgctacta
    11761 tattttcaac cgctccatca gaaaacggag taccttggga atatgctttg tttgaaatga
    11821 tgcccgtttc ggcggttata accgtactaa caaaatatca aagcgatatt cgaatggcag
    11881 aagccgaatt aatccagtac ctcaaagcac aaactgacgt ttccgatttc agggttaata
    11941 aaattgaagc cttagttgtt ccaaattctc gttacgtatt tagaggaagc aaatattcgg
    12001 ctcaaattgt tctttcagcc gttgattcca ctaaaacgcc cgagttctac gtaggtaata
    12061 cccctgtaaa aaatggaatt tatgaagtta attgcaatca gacaggtagc tttacttata
    12121 ctggtttcgt tcagttaata gggaacgatg gtgtgataag aaagtttccc tttacaagtg
    12181 attatattgt cggtgagcct tcggcaacca tttccaatga agatctgaat gttgtttatc
    12241 gcggcatcga ccataaattc agtatctctg ttccgggagt agcctctgaa aatatttcag
    12301 taagggtggc gggagcaacc gttcaaaaaa ctgctggagg taaattcatt attcgtccca
    12361 cacaggatgg ggaaatcaat gttgctgtgt atgccaaagt tgaaggaaaa gaaattttaa
    12421 tgggtaacag tatttataga gtgagatatt tgcctgatcc aaaagcttat ttacaatatt
    12481 cggatgccgg tggaattaca agattaatac aggatggaaa tcttaataag cgtttattga
    12541 gaggcgacaa tgtagccatt gtggcaagtt atggtgaaga cgaactcatt caggcaaaat
    12601 tcaatattat ttcttttaca atgctcacta tatatggttc agtagaaacc accggatcaa
    12661 ggttaagtaa tagacaattg gctgatattg atcgtttgga gggtggagat tatattacat
    12721 ttaaaaatat taaagccgtc ggtcctgatg gcaaagtaag aaatcttgga ttagttcaag
    12781 tacaaattta gttttttaaa attttttgca aaaataagca aaccaaatag aatgttcagt
    12841 atgaaaaaaa ttggtgttct aactttattc ttggctttta ttctactacc gggttccata
    12901 aaagcccaaa ttaatgaaat tccttttttt gatagcaaag gaattgttaa cattgaaact
    12961 accgagctcg atacattggc ggacaccatt gcagttgttc atcatcgtag agacgatatt
    13021 gtttggtcga gagtggtata tagggttata gatatgcgag aaaaacaaaa ctatcaacta
    13081 tattttccaa ctcgacccaa taaggaatat cgcaatcttt ttacggtaat gatcgacgca
    13141 atttgtgatg gagttaacgt gtataaaaaa aatccgcgcg atatcaaacc tgaattcaac
    13201 gaattgttgg aaggggaaga attatccagg gcttttgctt ttgaagaaga caataccagc
    13261 aatcttattc aaatagatcc cataaccaat cagcgaacct tcaacaatta ccgttatatt
    13321 cagtacgtgc gcaatcagtt gaaatatctg attcaggaaa ttattttctt cgataagcat
    13381 acctccagaa tgtattccaa aatcattggt attgcccctc tttatgcttt acatcccgat
    13441 aatgtcgaat ccaatgagac aatgggatat ttcagaggtt ccgttctctg ttggtttgca
    13501 tttgacgaac tgcgtcccta tctggccaaa caatatgtta tcccaaatgg gaacgacacc
    13561 caacgcatga cttttgacga ctttttctcg caaaaactct atgcaagtta cttgctgggc
    13621 gacagcaaca tgtttaacag aatgctgctc gattatgtta ttgaccctgc caaagttaga
    13681 aaagaacaaa acaggataga aaacgaattg ctgaactttg aacaggattt gtgggaatat
    13741 tgaaaaaagc tttcaaatat tattcaaata ttcaaaaaga cggctgttga gtcgtctttt
    13801 tttgcttaac gtatgaaact acattgcaaa aaattttttt ttaaagtaaa ttttatcgaa
    13861 caagtgaaca aaattattct taaacgatta taatcaaaaa aacacaaacg tcgtaaagat
    13921 tttttaagaa aaaagttatt cataaaaaaa tgaagaatat catactttcc gataatcaat
    13981 atatttctaa agccgggtgg ctttatctga tctcaaccta ctctctggag gcaaccatta
    14041 gggaagcagc cacaaaaaaa gaactggaaa aactccttgt tgaagagcag gaatcattgg
    14101 taatattgga ttatacactt tttgattttg aaaacgtcaa tgaattgata atatggagca
    14161 tgcgttttga aaaaaccgag tggttattat tttccgaaga attaagcgaa gaatttcttc
    14221 aacaaattat ttacaacacc cactccttca gtgtactcac aaaaaacagc aataaagaag
    14281 aaataaatct ggcattaaaa gaaacattgt ctggtaacag gtttatttgt aactttgtat
    14341 ccaatttgct gattgactgc caaaaacatc tgaaaaatga tggcaaaccg gttttaacac
    14401 ctactgagcg tgaaattctg aaagaaattg cactgggaaa aaccacgaag gaaatagcat
    14461 ccgagcgttt tgtgagtgta cacaccatta tgacacacag aaaaaatatt ttccgcaaaa
    14521 taaaagtcaa taacgtacac gaagccacaa aatatgcaat gcgggctgga attgtcaatc
    14581 tggctgaata ttacatctaa aatcaggcta aaaaatgaaa atagcggctt tactttccgg
    14641 aggggttgac agttcggtag tagttcattt gctgaaggag cagggttaca ctccttcgct
    14701 gttttatatt aaaatcggaa gagacgagga cgaaatgctg aattgctctt cggaagagga
    14761 tatagaaatg gcttcactcg tagcacagaa atatgattgc aagctcgaaa ttatcgacct
    14821 gcacaaggat tattgggaaa ctgttgttgc ctacaccata agcaaggtga aggaaggatt
    14881 gaccccaaat cctgacgtga tgtgcaacaa actgataaaa tttggcattt ttgaacaaaa
    14941 agttgggaaa gaattcgaca aaacagccac cggacattat gccaccatca ccgaaaaaga
    15001 tggaaaaatt tttctttcca ctgcaaaaga tccgataaaa gaccaaacgg attttcttgc
    15061 acaaatcgat tatctgcagg tttcaaaact gatgttcccg attggacact tcatgaaaag
    15121 tgaagttaga agcattgcac aaaaggccgg tttgccaagt gccaaaagac aggacagcca
    15181 gggaatctgt tttctgggaa aaatcaatta caacgatttt attcgtcgct acttgggaga
    15241 aaaaccaggt cctattgtgg aatgggaaac gggaaaaatt cttgggaagc acaaaggata
    15301 ttggtttcat accattggac aacgcaaagg tcttggacta tcgggagggc cgtggtatgt
    15361 gataaaaaaa gatattgctg ccaatatcgt ttacgtttcc tgcggatatg atgtagaaac
    15421 acaatatgga aatgaatttt cgatgcgcaa ttttcatttc attaccgaaa atccgtggca
    15481 aaaccaaacg gaagaagcaa atataacttt caaaatcaga catacacccg aatttacgaa
    15541 aggaaaaatt ctgcatacca acagcggctg gcatgtgatt tccgaaaaaa aaattcaggg
    15601 tattgctccg ggacaattcg gcgtgattta cgacaaggat tcccatattt gcatcggcag
    15661 tggtgaaata ttctgaaaaa acatcccgtc ttttttcaca caaatgtaac catcaattat
    15721 ttcaataaac aaattctgca gatgacttac cagacccata cgcttcctaa tggtttacga
    15781 atcatatatc tccaaaagaa aagtgaagtt accttttgcg gcattacgat aaatgccggt
    15841 tcacgcgacg aaaatcggga agaagaagga tgcgcgcatt ttgccgaaca tttgttgttt
    15901 aaaggtacac gaaaaaaaaa tgcacgagaa atcatcaacc gaatggaaga catcggagga
    15961 gaactgaatg cctatacatc caaagaagaa acagtggtgt atgcagcggc catgaaaaaa
    16021 tacacccgtc gcatgattga attgatggcc gacatgaccc tacattccac ctttcctcaa
    16081 aaagaactcg aaaaagagcg ggaagtaatc ttggacgaaa tagcgtccta tcatgataat
    16141 ccgtcggaac ttatttttga cgatttcgaa gaattgattt ttaaagatca tcctctgggg
    16201 cacaatatat tgggaaacgc aaaattattg aaaaagatga attcggaatc agtcagtaat
    16261 tttgtaaaaa gatgctatca accccatcaa atgattgttt ttgtggttgg aaacctgaat
    16321 tttgaacact ttgtaaaatg gtgtgaagaa tattttgtat cggaaagtgc agaaaatatt
    16381 aacgaaaaac aaaaacgcaa tccccctgaa caatatcggg cagaaaaatt gaagctgaag
    16441 aaaaagaccc atcaaatcca taccgtaatt ggaaatcgct gtttcgattt gtaccatccc
    16501 aacaggttgc ccatgtattt actcaacaat atacttgggg ggccgggaat gaatagtttg
    16561 ctcaatttat cgctgcgcga aaaacacgga cttgtttata ccgcagaatc gaattatcaa
    16621 ccttttaccg acagtggatg gtggtgtgtt tactatgcat gcgacgaaag aaacgacgaa
    16681 aaatgtgaac agctggtttt ggaacaactt caaaaaatcg ttgaccgacc cttgccggaa
    16741 agtaaactta gaaaatacaa acagcaactg atcggacaaa tgactgtttc aagagaaaac
    16801 actgaaaatt ttgcacttag tttgggaaaa agttatttgc gcttcgggaa agtggaagaa
    16861 atggaagaaa taaaaaaaca gttggagaat attcaaccta aagatttaca atacattgct
    16921 caagaaattt ttaatgaaga gcaactatcc gttttgaaat attattgaaa ttatcttcta
    16981 caaaaaaaaa tggatcgaaa atccgaaaag gaaaaaccga gtttgctttt ttgcctgatt
    17041 atggatgtat tgggttgcat tactacatta tgccttgaaa aataatttcc atttaccttt
    17101 tatttcctct gaaaaaagtt gcattaattt cttgaattta cgtcttttat tctgtctttg
    17161 agaaacggat attcgttcag gatattgttt ttttccaatt cgatttgttg tagaaaagaa
    17221 acatactccg gtgaattggg atgcagtggg cgatgattca ttttttccct caacatttcc
    17281 aatcgttgtt ttatttgttt tgttttctcc gaaaaataag tatccgaaaa tttttcccaa
    17341 atgtaattga tggcggtttc cgaaggatgt aacatgtccg aagcatagaa ccgataatca
    17401 cgcaattcat ccatctgaat ttcataggca gggaaataat caacattttt gaattttttc
    17461 tgcaattcct ctatcgataa cagcaaaatg cttttactta agttgttttc gtgagcgcca
    17521 tctttccaat ggcgtatagg gctgactgta aaaatgagat gaagcgatgg attcaattgt
    17581 tgaagttggg cgatgagcaa agtgtaatgt tccactattt cttcaatgga tagccttcgg
    17641 cggttgaaat gttgtgaagg tagcttatgg cagttggaaa ctacagtctg tgttttccgg
    17701 tcttcaaaaa cccacgctgt gccaaaggtc agcaaaagaa aatctgtatt tttcagaaat
    17761 gaacaagatt catcaaaccg ggcttgaata ttagagagtg ttttttcggg tgaaacatca
    17821 gaaaatgaac tactgtgaaa aaaactatgc caaagacatt ggcattcaaa aatatctgaa
    17881 cgtgaaaaac ctttgttttc aattaatctt tcaagactca aagagatgga aagcggattg
    17941 taaagcactc cgaaaggatt gacgtcaacc tgaaagaaag cttcatgaag ttgtttacct
    18001 atattttcag aaaagcaaga gcctaacatc atgattttat ccttataagt aattttttgt
    18061 tgggatggag caagttcaac aggggttcta aaaagatagt tagtcataaa atttacttta
    18121 tttttaaaaa tttgtcagat ttcatattag ctttaacaga ttataaacgt attttccgta
    18181 aaaaatcatt tatttatttt acaaaagtaa taaatgttca tagttattgt tgtaaatttg
    18241 tagctctaaa aatgatatac aaaatatgaa aaagaacaaa aacatcttat tgtttatctg
    18301 ttttttatcg atttcttttt tttgggggtg tgtgtcaaaa acagttaaaa caaaaaattt
    18361 cgagtatgcg aataaatttt acctcaccga tgattcaaca aaaggagctt taaatttgat
    18421 tattaatgtt gagataccag taaatttttc taataaaacc gttttggatt cagttcgcaa
    18481 taaaatattg ttgtttctat ttggagaaaa ttatgtgaac atgcctttgg attcggttat
    18541 cccagccttt gtccgagaac aaagttccga ttatcgaaat aacaatcttt ttatgttgaa
    18601 tacactcaat ttgcagaatt attttgcttt caacaatgac catattttgg aaggattttc
    18661 gttgtggagt gacgaaaata ttttttcata cggaataaaa cgttacgtat tcatgggtgg
    18721 agctcacgga ttatcaacac agactttcct gaattttgat ttgaaaacgg gaaaaactct
    18781 tacagaaaaa gacttgttta ttcctgatta taaagaaaaa ctgactactt tgattcgtga
    18841 aagactgatt gaggaaaatg acgaaatttc ggaatataga gatttgaatc aaaccgatta
    18901 ttgggtggac tccatacagc ctaacgggaa tttttacatt tcggatgaag gaatcaacta
    18961 tttgtttaat ccttatgaaa ttgggcctta ttatctgggc gaaacagaag ttcggattcc
    19021 ttatgctcga ttaaaagata ttttaaaaaa tggtaatccg ttggagtatc tatatgaaaa
    19081 tcggtaagtt aaatgaatat gaattgtttt ttcaatttgg ccaaaacgcg gcggagcatt
    19141 cgcaaatttc ttcctcaaaa agttgaaccc gaaaaaattt cgatgataac aaaagtggct
    19201 ttgatggctc ctgcatcgaa aagaagcaat ccctgggagt ttgtggtggt tcaacaacct
    19261 gaaatgttac aaaaattatc gcagtgtcgt cccaaaaatt cacaatttct ggcgggggct
    19321 ccgttggcaa ttgttgtgct cgccgatacg gaaaaaagta atgtttggat ggttgatgct
    19381 gctatcgctt ccattatgat gcagttgcag gcacacgatt tgggattggg cagttgctgg
    19441 attcatgttc atgaccggca aatggatgaa aatacaagct cggaagatta cattcgcggt
    19501 ttgctacaaa ttcctactca gtatgcaatt ctgaatattc tggctgtggg ttatcccaac
    19561 gaaacaaaaa atccgcacga cgaggaaaaa ctggcttacg aaaaagttca tttcgaaaaa
    19621 ttctaacttt atttttaaaa tatggcttcc aaaaagaaaa gcagtaatct ggcctgggga
    19681 ataacattac tgttatttgg cttgttgttt cttttgcgtc aattgcagat tgttccgccc
    19741 gaaatcggcg tttttgtttt tgatattaaa aattttccgt ttattgccgg agttattttt
    19801 cttctttttc atcccaatat cagtatcggg atagttttac tcgcagtagg tgtgctgttt
    19861 cggctttcag aaattattgc atggacaaaa aatttttcgg aatttattct tcccgctttg
    19921 ctgataattg ccggtagcat attgattttt agtaaaagaa aataatcaat tgccacaggt
    19981 aagtcaaatt ttttgtaagt atctattcct taacgctttt aaattctgaa acagttatag
    20041 ttttttcatc atgaaacggg ttgttattat cggagcggga aatgtagcta ctcatttggg
    20101 gcgagcgtta caaaataaga attttgttgt gagtcagatt tttagccgta ctgaaaattc
    20161 tgcaacacag cttgcttctg acttgggagt tcctttcaca acaaacttgc aatcactcga
    20221 cagagaagcc gacatttatt tttattgtgt aaatgatgcc gtattgtgcg acgtcatcaa
    20281 taaaatcgat attcccgacg ctttgcatgt tcatacggcc gggagcttgc ctttaagcat
    20341 tttcgaaagc catctgcaat accgtcgata tggagttttt tatccacttc aaactttttc
    20401 aaaaacaaga aaagttaatt ttgatgaaat tcctattttt gtggaaggga attcattgga
    20461 agcagaggaa gaaatcaggc aaattgcgca aacattatcc aaaagagttt tttcttccga
    20521 ttcggaacaa cgaaaaaaaa tccatctttc tgccgtattt gcctgcaatt ttgtcaatca
    20581 tcttttttcc attgcagaag aattggtgaa aactgccgga tattcctttg atattttgtt
    20641 gccgttgatt actgaaacag taaataagct cagcgagttg tctccttacg atgcgcaaac
    20701 cgggccggcc gtgcgaaatg atcaaaacat tattcgtcag catttgttgt tgcttgaaaa
    20761 taaacccgaa ttttcggaaa tttacagcgt gttgtccgaa agcatttatc ggatgcacaa
    20821 aaaatatatt caaacaagag aatagtcaat tttattgtaa atcagaaaat tgccttttct
    20881 ttttatcagc taaattcaat atacatgaag caatatttgc aattacttcg gttaccaaat
    20941 ttacttttta tcgcttttgt tcagtttgcc atgtccgaag cggttatttt gccgcttttg
    21001 atgacctatg gttttgatgc cggcatgagt tcatccaaac ttttgttgtt gatattgagt
    21061 acgatattaa ttgctgccgg aggatatgta ttgaacgatt attttgacgt aaaaatcgat
    21121 accatcaatc gtcccgataa aatgattgtg ggaaaaacca ttccccgtgc ttctgccatg
    21181 cggtattatc aaattttgac ggctctggga ttgttgtcag ggtttattct tgcctttctt
    21241 gtaaaaagtt ttgctcttgg ctttattttt ctggtggttc cgggtatgtt gtggttttat
    21301 tcggccacct acaaaaggca gtttctgacg ggaaacatca ttgtagcttt tgccagtgct
    21361 ctttccgtac ttttgcctgc cattctcgaa ctggctgtat tggaaagtca atacggaaaa
    21421 ttaatttttg aaacgcctat tccgaaacag atttatgctt gggtaggagg atttgccgtt
    21481 tttgcttttt tttgtacttt attgagggag attataaagg atgcggaaga tattgccggc
    21541 gaccgggaaa tggaatgtca taccttgcca attgtttggg gaataaaaaa gaccaaaatt
    21601 attgtttatt gccttatttt gctcattgtt gccggcttgc tcgcagccga ctttttcctc
    21661 attgatttta g
//
LOCUS       DAOSCH010000022        21167 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1774_length_21167_cov_6.161425, whole genome shotgun sequence.
ACCESSION   DAOSCH010000022 DAOSCH010000000
VERSION     DAOSCH010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 21167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21167
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1774_length_21167_cov_6.161425"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..416
                     /gene="ffs"
                     /locus_tag="PLZ54_03110"
     ncRNA           317..416
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLZ54_03110"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(452..1780)
                     /locus_tag="PLZ54_03115"
     CDS             complement(452..1780)
                     /locus_tag="PLZ54_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOJ65959.1"
                     /translation="MASPINFFVRIYNFFVSDIWKIAENELTRTRRFFYHLIKIILYA
                     IRGYSTDKLAIKASALTYSILFAVVPVIAIIIAIGRGFGMETLIEDALKNTFIAQANL
                     VPVVMDFVKRYLESIQGGVFLGVGIVILLWAVVSFFMQVEDTFNSIWQVKKSRSFLRQ
                     FTTYFSIILILPLFIVLSSGFSIFVNTTISQIPGFQILNPLLKVGANLLPYLINWLIF
                     TFMYLFIPNTQVRFINALIAGIIAGTAFQLFQILYITGQINLSRYNAVYGGFAAIPLL
                     ILWIRISCLIVLLGAELTYASQNIRNYESELDTQNISIRNRKLISLYITYLVIKQFEL
                     QKPPLSSEQIASDFKLPIRLVNQILNQLVNISVLIEVFNDKNKSKSYQPAMDINQLTV
                     GLLFEKLESYGTGITLLKNNEKLKNFWEKTSAVLAAGSKESSNEILIKDL"
     gene            complement(1865..2222)
                     /gene="rnpB"
                     /locus_tag="PLZ54_03120"
     ncRNA           complement(1865..2222)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLZ54_03120"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2296..4122)
                     /locus_tag="PLZ54_03125"
     CDS             complement(2296..4122)
                     /locus_tag="PLZ54_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004325559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOJ65960.1"
                     /translation="MEYQLTDFLPVTRKELELRAWEDVDVVFFTGDAYVDHPAFANAL
                     IGRLMESLGLRVAIVPQPNWRDDLRDFKKMGRPRMFFAVSAGNMDSMVNHYTANKRLR
                     SNDAYSPDGKPGFRPDYATVVYCNILKKLYPDVPILIGGIEASLRRLTHYDYWSDQLK
                     QSILCDAQADLLIYGMGELPLTELIRQLKNGKQFHELTDIPQTVYLSDNIPQDNLWNT
                     IELPSFETCLHDRKKYASAFKTIEEQSNRYQADRLIQKTEDKWVVVNPPYPPMIDAQL
                     DASYELPYTRMPHPKYKGKTIPAYEMIKFSVNIHRGCFGGCSFCTISAHQGKFIVSRS
                     KKSILNEVEKIKLMPDFKGYLSDLGGPSANMYQMKGKNIEICKKCKKPSCIFPKVCFN
                     LNTDHHKLLDIYRSVDALQGIKKSFIGSGIRYDLLLHESADKLTKKSQSEYIRELILH
                     HVSGRLKVAPEHTSDRVLAIMRKPSFQYFIRFKTQFDRINKEANLNQQIIPYFISSHP
                     GCTKEDMAELAVITKNLDFKLEQIQDFTPTPMTLATEIYYSGFHPYSLQPVYVAKTKE
                     EKLEQRKFFFWYKPEYRSQIKRDLLMIQRPDLLKKFFQNERK"
     gene            4439..4753
                     /gene="rplU"
                     /locus_tag="PLZ54_03130"
     CDS             4439..4753
                     /gene="rplU"
                     /locus_tag="PLZ54_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOJ65961.1"
                     /translation="MYAIVEILGQQFKVQAGKKLYVHRMEEAEIGSQVEFDKVLLVDN
                     DGSIAVGAPCVEGAKVVCDVVGHVRGEKVIVFHKKRRKGYRKRTGHRQDFTELLIKEI
                     VA"
     gene            4773..5033
                     /gene="rpmA"
                     /locus_tag="PLZ54_03135"
     CDS             4773..5033
                     /gene="rpmA"
                     /locus_tag="PLZ54_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOJ65962.1"
                     /translation="MAHKKGVGSSKNGRESESKRLGVKIYGGQFAKAGNIIVRQRGTV
                     HHPGKNIGMGKDHTLYALVDGKVEFRKKVGNRSYVSINPEMN"
     gene            5209..6480
                     /gene="serS"
                     /locus_tag="PLZ54_03140"
     CDS             5209..6480
                     /gene="serS"
                     /locus_tag="PLZ54_03140"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOJ65963.1"
                     /translation="MLTLKYIAENSEDVLKRLKKKHFDGKELISKVLELNAERKKTQY
                     DADSIAAEINKISKEIGILYKQGKTSDAETLKQKTLELKENIKLLEEKQNQIEAQLNE
                     ILVQIPNLPHESVPEGKLAEDNQVERTGGKIPSLPTDALPHWDLAKKYDIIDFETGVK
                     ITGAGFPLYKGKCARLQRALINFFLDNAREAGYMEVQPPYVVNEASAYATGQLPDKEG
                     QMYFCQEDNLYLIPTAEVPVTNIYRGMILNENELPIKNVAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQIQHPEKSYETLYEMVHYVEGLVEKLELPWRTLRLCGGDMSFTSA
                     LTFDIEVYSAAQQRWLEVSSVSNFESYQANRLKCRYRSADKKIELCHTLNGSALALPR
                     IVAALLENNQTPEGIRIPKALIPYTGFEIID"
     gene            6686..7594
                     /locus_tag="PLZ54_03145"
     CDS             6686..7594
                     /locus_tag="PLZ54_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOJ65964.1"
                     /translation="MESISSFAELIAHLKFLNNRKRVAVANAVDPHTLEAVLRASDNG
                     FVEAFLIGDLAMIESPHFFEHKLTPYVHIIDIPEVEEATREAVRMVRTGEADILMKGL
                     VNTDVILRAILNKENGLVEKGKIITYNAALEIPDYQKLLFFTDPAVIPSPTVEQRTAM
                     IKYVTETAYKFGITRPKIALIHATEKANPKIQYMQDYLDIMEKWRMGEFGDVIMDGPL
                     DIFLALDPERGSIKKVSSPIHGDADILIFPSFECANAFYKGLMVFAKAEMAGLLQGTQ
                     KPVVLTSRSESVNSKYYSLAMACVMG"
     gene            complement(7584..8738)
                     /gene="lpxB"
                     /locus_tag="PLZ54_03150"
     CDS             complement(7584..8738)
                     /gene="lpxB"
                     /locus_tag="PLZ54_03150"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOJ65965.1"
                     /translation="MKYFLIAGEASGDLHASHLMHELKKQDLQAEFCFLGGDLMWTEG
                     GEMIRHYRQMAFMGFIAVLKNLSVISVNIKDGKQALLKFQPDVLILVDYPSFNLRMAE
                     FAKKKLNIPVYYYISPKIWAWKEFRIKKIRRYVDRMFTIFPFETEFYAKHRYPVEYVG
                     NPTVDAVEEFKKAYRGNFQTFRQSNNLVDKPVIALLAGSRRQEIKSCLPRMVEAAMQF
                     PDYQPVVAGAPGVEKDFYFSVLKDTSVAVVFGQTYELLFHAEVAIVNSGTATLETALI
                     GTPQVVVYHVAFGKVAYWLKKLFIKVKYISLVNLIAGKEVVMELYAHLFTVENVKNEL
                     NRILTDAHYRQNMKNEYKKVSEILGEPGAASRAAKKIFELLHSDKSVLTP"
     gene            complement(8788..9072)
                     /locus_tag="PLZ54_03155"
     CDS             complement(8788..9072)
                     /locus_tag="PLZ54_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65966.1"
                     /translation="MKRLNHFYIGFLVGLILPCVFVWLYITSFYPVDISFFEILKRLY
                     PGVLLGKLLLLSIVPDLLMAFVFYKNDAFRLTSGTIVGGLPFLIASLFML"
     gene            complement(9084..9842)
                     /gene="surE"
                     /locus_tag="PLZ54_03160"
     CDS             complement(9084..9842)
                     /gene="surE"
                     /locus_tag="PLZ54_03160"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOJ65967.1"
                     /translation="MNKNLILISNDDGIDAKGIKILTKLMMQLGDVVVVAPDIPRSAQ
                     SSALTVTQPIRYRKIEEEEGMVRYSCTGTPTDCVKIALNEILNRKPDLLVSGINHGSN
                     SAINVIYSGTMGAVFEGCENGIPSIGFSLASHFPDPDFSFFEPHVLKIAQIVMEKGLP
                     ESVCLNVNAPCGEIHGVRVARQCKGYWTKEFAARTDPAGRAYYWLTGYFENMEPDAED
                     TDEWAISHGFISIVPDKIDLTAYDAMDLIREWNL"
     gene            complement(9916..10647)
                     /gene="gldF"
                     /locus_tag="PLZ54_03165"
     CDS             complement(9916..10647)
                     /gene="gldF"
                     /locus_tag="PLZ54_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOJ65968.1"
                     /translation="MISIFKKELGSFFSSATGYLVIGIFLALTGLFLWVIPGEFNIIE
                     SGYANVDGLFYLAPWLFLFLCPAITMRSFAEEKKSGTWEILITKPLSITELVLGKFLA
                     GWVLVLIALFPTVLYYFSVYFLAEPVGNIDAGAFWGSFIGLIFLSGVYIAIGIFSSSL
                     SNNQIISFVVSVLLCFIFFYGFDLLGSFFSSGAVIDFVQNLGINAHYKSMSRGVLDMR
                     DVGYFLFLGGLFLGIMVWKNSKSDK"
     gene            10808..11980
                     /locus_tag="PLZ54_03170"
     CDS             10808..11980
                     /locus_tag="PLZ54_03170"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOJ65969.1"
                     /translation="MFFAIYSFGFAQTFKNYSLSADFIYGEILKHNKHLENLVKGPLK
                     GEEFSIEWQTMGEKAWHQYLNFPSVGISTAFLDFSHPDTLGYAVALYPYLKFPIVRQQ
                     HFNMAFKAGGGLSYVTKTFYNATAYHPDGSVYLNKSNAAIGSHVNVYLTANLNMEIPL
                     TKGWSITSNWGWNHTSNGSIVQPNSGINMLDMYLGIKYFPNYERYIPPKPQPLPDLPH
                     KIEIEMAFTGGIRQLYYKDDKTYPIGTFNIAAYRTMTNFYRMGWGADLFYDGVFNGKS
                     AFKRTYLTTDELKYKLRIGASFRHELLVGRLTTGFHFGLYLYNPVKNLEPYEEAKNGT
                     LNKPLIYKYNIEKEDGWFYTKAVLKYALDEHFLLSLGLKTHLQKAEFIDAGIGYKF"
     gene            complement(11999..14212)
                     /locus_tag="PLZ54_03175"
     CDS             complement(11999..14212)
                     /locus_tag="PLZ54_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ65970.1"
                     /translation="MNATQIDTTYKAILRFVSASRLKPAFDRIKMMIDDLQYGEFSDR
                     FNELERNYKFLLDYYLKGVQDPERKSVYSKLVAKLLILLADVREELMMKNSSNFEYSQ
                     KRFFPYTRKYQTVNELFNALQYFHKQSALLSENKEQYKIELSRMRSNFEDALDELFGV
                     FWLATRYTEEENNLYKKLLEADDKYWVEKSLMVSAVTLNLWRKFDEQKIMLLFDSCEN
                     ADQKVVQRALVGLTFILAKYNIYLPYFPSIRNRLVLLVDQNRIAENFQNIIIQIIGTT
                     ETEKISRKLREEILPEMMKINPLLRDKMDAENFLNSDEWAEANPEWQEIFDESGLSDK
                     LKELSDLQLEGADVYMSTFSMLKNFTFFSQFSHWFLPFDTENSAVNQLFESDEKTILS
                     AFVNSNVMCNSDKYSFCLSVLQMPESQRNMLKHSFKMESDQMDEMEEEMSILSPETIT
                     KNISKQYIQDLFRFFKLQPHKEDFEDMFSYSLTLHRTWLFDILSSIPGFSESVAEYYF
                     SKGYYLQALELYEKIQSESQPSAALYQKMGYAYQQTSQLQKALDAYLKADMIQPDDGW
                     TIRKIALCYRLLGNYEKALAFYQHADFLKPGQYSTQIHIAQCYFELGKFKDALAIYYK
                     LDAENVDDIKVWKAIAKCNFFDGNLDKADYYSEKIILAEPNAYDYLLSGHISWCRRQL
                     SAAANAYRKALEMLQNKWEVFQDLFNEDKDRLISFGIREEELPLMMDALLYYENE"
     gene            complement(14288..14989)
                     /locus_tag="PLZ54_03180"
     CDS             complement(14288..14989)
                     /locus_tag="PLZ54_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ65971.1"
                     /translation="MTKKPAYELKPIEEVFMVWDVLTPDERQFVRNNYTVHHFKKNEL
                     IQSEGEISTHMMILAAGKAKVYKDGIGSRTQIIRMLKPGENFGYRAIIANEPNSTNVA
                     AFEASTVYMLKADLFLSILRHNIAVCYRFLEELASDLAVSDARTVNLTQKHIRGRLAE
                     ALLFLKKRYGVEEDGATVNIYLSREDLANLSNMTTSNAIRTLSNFTNEHIIAVDGRKI
                     KIIDEDKLRKISKMG"
     gene            15266..16513
                     /locus_tag="PLZ54_03185"
     CDS             15266..16513
                     /locus_tag="PLZ54_03185"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HOJ65972.1"
                     /translation="MKRAIFVSLLSFILFPILSQNFTFSDSAKVSLLTCSPGKKVYEK
                     FGHTAIRVNDPITGYDVVFNFGVFDFNTKNFYLKFIKGDTDYQLAVYDTKSFLEEYKS
                     RNSMVWEQKLNLTLNEKRNLLHSLLINYEPENKFYRYNFVYDNCSTRPRDKIVQSVNG
                     KIEYHLYNEPVTFREMIAQYTGVNSWMQFGLDLIFGRDADKSVSRNVSMFLPENLMYE
                     FQAAHIIAADGAKRHLVAENKILIPKQEEKYSKPLPIFQPVFVCTFLLILGIIITIFN
                     NKKSFRLFDSLLYIVSGLGGIIVFYLAFFSSHPLVKNNFNLLWLNPLNFALGILLWFH
                     KMKPYVFYYQVLNCLLIFLALVAYAAGLQVFHPASFPLILLLLLRTVRWTATTKKDFS
                     LKKKNNRDYYTNSNGLHHHSRKN"
     gene            16547..17320
                     /gene="kdsB"
                     /locus_tag="PLZ54_03190"
     CDS             16547..17320
                     /gene="kdsB"
                     /locus_tag="PLZ54_03190"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOJ65973.1"
                     /translation="MTLHSNRNFRFVGIIPARFASTRFPGKPLADIGGKPMIQRVYEQ
                     ARKVLQTVYVATDDHRIYDCVNNFRGKVLMTSAAAKSGTDRCYEAVSQLQENFDAVIN
                     IQGDEPFIQPEQIVTLQQCFEDEQTQIATLAKIVHQTEEYDYILNPNRPKIVLNKNNE
                     ALYFSRQVIPYQRNTANEHWISNHQYFVHVGIYAYRTEILKELCSLPVSSLEKAESLE
                     QLRWLENGYRIKVGFTTAESYGIDTPEDLEKVRKMFLGK"
     gene            complement(17689..18720)
                     /gene="mltG"
                     /locus_tag="PLZ54_03195"
     CDS             complement(17689..18720)
                     /gene="mltG"
                     /locus_tag="PLZ54_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOJ65974.1"
                     /translation="MKKKVRKILFAALAVPVIVVVYLTYVFFAPNIFPKKETETFVCI
                     PENTSFEDVVKLIKNESRVWDSYSLRLTAKIFDYDKNIRSGRYALKKGMNNFALIRNL
                     RSGRQVPLRLTFNNIRTKEQLAGRLSSQLMTDSLTFLKLLNNSQFLSQYALNPDNSIV
                     IFIPDTYEVFWNIKPEKLFDRMYREYLKFWNEERRQKAASIPLTPIEVSILASIVEEE
                     SNKSYEQPIIAGVYINRLKNKMPLQADPTIKFAWKDFSLKRIKGQHLQINSPFNTYKF
                     QGLPPGPIRLPSPQVIDAVLNYTHHSYLYMCAKETLNGEHNFATTHAEHLINARKYQR
                     ALNERQIFE"
     gene            18924..19010
                     /locus_tag="PLZ54_03200"
     tRNA            18924..19010
                     /locus_tag="PLZ54_03200"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18958..18960,aa:Ser,seq:gct)
     gene            19119..20486
                     /locus_tag="PLZ54_03205"
     CDS             19119..20486
                     /locus_tag="PLZ54_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004302195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOJ65975.1"
                     /translation="MEKTKKFILTEKELPTHWYNIVAEMQNKPMPILNPATKQPMKEE
                     ELYPLFAKELSHQELNQTDAWIEIPEEVREKYKIYRPTPLVRAYDLEKALDTPAHIYF
                     KNESVSPVGSHKLNTALPQAYYCKQEGVTHLTTETGAGQWGTALAFAAKTFGLELFVY
                     MVKISYEQKPYRRSLMQTWGAQVIASPSMSTNAGRKILSEHPNYLGSLGTAISEAIEL
                     AMNTPNCKYTLGSVLNHVSLHQTVIGLEAEKQMEMAGEYPDIVIGCFGGGSNFSGISF
                     PFLRHNIKDGKKTRFIAAEPASCPKLTRGVFEYDFGDEVGYTPLLPMFTLGHNFAPAH
                     IHAGGLRYHGAGTIVSQLKKDNLVEAVDIDQLDSFKAGVLFAQTEGIVPAPESAHAIA
                     VAIHEALKAKEEGTPKTILFNLSGHGMVDMTAYDQYFAGDLTNYSLSENDIAENIKNL
                     EHLAK"
     gene            complement(20568..20801)
                     /locus_tag="PLZ54_03210"
     CDS             complement(20568..20801)
                     /locus_tag="PLZ54_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65976.1"
                     /translation="MIESSAISISVAIGNSRYLPAALDELSNEFDVSYNENLELLTVR
                     GISDEIAQEVTNHREVLLMQRTRRIGRFLMKER"
     gene            complement(20808..>21167)
                     /locus_tag="PLZ54_03215"
     CDS             complement(20808..>21167)
                     /locus_tag="PLZ54_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOJ65977.1"
                     /translation="WKDVPGILNADPRLYPQAKLVPELTYSEAVELAYSGAQVIHPKT
                     IKPLQNKNIPLYVRPFGNPSEKGSVIKAHVEKPAGIPFYSLLKNQVLVTIRPIDFSFV
                     LEENLPKVFSMINKHRL"
BASE COUNT         6960 a         4234 c         3796 g         6177 t
ORIGIN      
        1 tgtattctat gttactgctc aaaaattggt gttatttctc cgtaattatt tccatatact
       61 cttaaaacta tacctccatg ctgccgcaaa actttcgtgt ggtactgcgg tagtacatga
      121 aactgatttt gtcccggagg gacaaccttt tggtagaaaa aggcaatgaa caaaaaaatc
      181 ccgtagggat gaccagagga atgatgttga tttaaagttt tgccacacga gcaaattttt
      241 tttgtcatgt gctgtatgct gaggaaaaaa tacagagata tttggaaata tgataaaaaa
      301 tcgtaccttt gcaacggggt aagtcctaca cgaccagctc ctttcaaact cccccagggc
      361 ttgaccgcag caagggtaga aagttgtagc ggtgcgatgt agggagcttg cccttttttg
      421 tttcaataca ttaatgttgt aaacaaataa tttacaaatc cttaattaaa atctcattag
      481 aagattcttt actacctgca gctaaaacag cggacgtctt ttcccagaaa tttttaagtt
      541 tttcgttatt ctttaaaaga gttattcccg ttccgtaaga ttccaacttt tcaaacaaca
      601 atcctactgt taattgattg atatccatcg caggctggta acttttagat ttatttttat
      661 cgttgaaaac ttctattaat accgatatat tgactaattg attgagaatc tgattcacca
      721 aacgaattgg taatttaaaa tcggaagcta tctgctcgga agataagggt ggtttttgca
      781 attcaaattg tttgataaca agataggtaa tgtataaaga aataagcttt ctgtttctta
      841 tgctgatatt ttgagtatcc aattctgatt cataattacg aatgttttga gaagcataag
      901 tcaattctgc tcccaaaaga acgataaggc aagaaatgcg aatccaaaga atgagcaggg
      961 gaatcgcagc aaaaccgcca taaacagcat tgtaacgcga caaattgatt tgtccggtga
     1021 tatacagaat ttgaaatagc tgaaaagctg tccccgcaat aattccggca atcaaagcat
     1081 tgataaatcg tacttgggta ttggggataa ataagtacat aaaagtaaaa atcaaccaat
     1141 tgattaagta gggtaataaa tttgctccaa cttttaaaag aggattcaaa atctgaaaac
     1201 cgggaatttg agaaatggta gtgtttacaa aaattgaaaa tccgctggaa agcactataa
     1261 aaagcggtaa aataagaata atggaaaaat aggttgtaaa ctgacgaaga aaggaacgcg
     1321 atttctttac ctgccaaatg ctgttgaatg tatcttccac ctgcatgaaa aaactcacta
     1381 ccgcccaaag caaaattaca atacccactc ccagaaatac gccgccctga atgctttcca
     1441 agtatctttt aacaaagtcc ataactaccg gcacaagatt cgcctgagct ataaaagtat
     1501 tttttaacgc atcttcaatc agtgtttcca ttccaaagcc acgtcctatt gcaatgataa
     1561 tggcaattac aggtaccact gcaaaaagga tggaataagt cagagcagaa gcttttatgg
     1621 ctaatttatc agtggaataa ccacgaatag cataaagaat tatttttatc aaatgataaa
     1681 aaaaacggcg ggtacgcgtg agttcgtttt cggcaatttt ccaaatgtcg gaaacaaaaa
     1741 aattgtaaat gcggacaaaa aaatttatcg gagaggccat aagcagatga atgatgattt
     1801 acaattttgt gaatttgaga tttaccacaa aaaatatcat tgaaaaatca gccacttttt
     1861 taaaaaaaat agcaggtttg taagccgggt tttgtacttt gcaaaagcaa agtgtttgtc
     1921 atttatctga agcaaatgtc gccacttgct ttaagcgatc tacccctcga catcggacga
     1981 gccatcctac atgcgtcgat atacatgatc ttgcaaccca taaggcgtac ggctaaaagt
     2041 gttgccactc ttaccggtga gctcttgcct caccttttca cccttacctc aaacaaggcg
     2101 gttgttttct gttacgctac tctgcccttg cggacagctt cccgttagga agtatggcgc
     2161 tctgtgttgc ccggactttc ctctttccaa aaaatttgga aagcgacaaa ccaacctgct
     2221 atcaacttac aaagttactg atttttatac acttttcaaa aaaaacggga aaaaataatt
     2281 gttatatacg ataagttatt ttctttcatt ttgaaagaat ttcttgagca aatcgggacg
     2341 ttgtatcatc aataaatcgc gttttatctg acttcgatat tcaggtttgt accaaaagaa
     2401 aaattttcgc tgctcgagtt tctcttcttt ggttttggca acataaaccg gctgcaaaga
     2461 atagggatga aatccggaat aataaatttc ggtagccagc gtcataggtg tcggagtaaa
     2521 atcctgaatc tgttccagtt taaaatccaa atttttcgtt ataacggcca gttcggccat
     2581 atcttccttt gtacaaccgg gatggctgga aataaaatac ggaataattt gctgatttaa
     2641 gtttgcttcc ttgttgattc tatcgaactg ggtcttgaac cgaatgaaat attgaaaaga
     2701 aggctttcgc attattgcca atacacggtc cgaagtatgc tcaggagcca ctttcaggcg
     2761 acctgaaaca tggtgaagaa tcagttctcg gatgtattcg gattgacttt ttttagtcaa
     2821 cttatcagcc gattcgtgca aaagtaaatc gtaacggata ccgctcccaa tgaaagattt
     2881 tttaattcct tgcaaggcat caaccgaacg ataaatatcc agcaatttat gatgatcggt
     2941 gttgaggttg aaacaaactt taggaaaaat acaggaaggt tttttacatt ttttgcaaat
     3001 ttctatgttt ttccctttca tttgatacat attggccgaa ggtccgccca aatcacttaa
     3061 ataaccttta aaatcaggca taagcttaat tttctctact tcattcaaaa tagatttttt
     3121 tgaccttgac acaataaatt ttccttgatg ggcagagatg gtacaaaaag aacaacctcc
     3181 aaaacagcct ctgtgaatat tgacggaaaa ttttatcatt tcgtaagccg gaatggtttt
     3241 ccctttgtat ttgggatgag gcatccgcgt gtaaggcaat tcataagaag catcaagttg
     3301 tgcgtcaatc attggaggat aaggtggatt tacaactacc catttgtcct cggtcttttg
     3361 aattaaccga tctgcctgat atcggttcga ctgttcttct attgttttga atgcagaagc
     3421 atattttttt ctgtcgtgca aacaagtttc aaaggaaggc aactcgattg tattccacaa
     3481 attgtcttgg ggaatgttat ccgacaaata aacagtttgg ggaatatcgg tcaattcatg
     3541 aaattgtttc ccgtttttta actgacggat taattcggtc agcggcaatt ctcccattcc
     3601 ataaataagt aaatcggctt gagcatcaca taaaatactt tgtttcaact gatccgacca
     3661 atagtcgtaa tgggtcagtc gtcgcagtga agcttctatt ccgcctataa gaattggaac
     3721 atcaggataa agtttcttta aaatattaca ataaaccact gtggcataat cgggacgaaa
     3781 gccgggtttt ccgtcgggag aataagcatc gttggaacgc aaacgcttgt tggccgtata
     3841 gtgattgacc atagaatcca tattaccggc cgaaactgca aaaaacatgc gtggtcgccc
     3901 cattttttta aaatcacgca aatcgtctct ccaattgggc tgaggaacaa tagctacacg
     3961 gagccccaac gactccatca gccttcctat caaggcattg gcaaaagcag gatgatccac
     4021 ataggcatca ccggtaaaaa aaaccacatc cacatcttcc catgctctta attcaagctc
     4081 ttttcgcgta accggcaaaa aatcggtcaa ttgatattcc atatatcttc acagtttcaa
     4141 caacaaaaat aatggataat tttcaattta tcctacgaca catgattatt aaaactttcc
     4201 tactcaattt ttttttcaaa ctgtaattgc aaattcgtcc aataaaccaa aaaaattcga
     4261 gtttctttaa aatctttcaa gtttttaaaa tcaaagaaaa aagtaaccgg aaaaatattg
     4321 agaaaaagat tgaaaaacca aaaaaaaatt gtacatttgc acctccattc agaaaaggtt
     4381 cttaagaagc actgctttag gcatctcacc ttactgattt aacaaaaaaa cgaataaaat
     4441 gtacgcaatc gtagaaattt taggacaaca atttaaggta caggcaggca aaaagctgta
     4501 tgtacatcga atggaagaag ccgaaatagg ctcacaggtg gagttcgaca aagtattact
     4561 ggtggacaac gatggttcaa tagctgtcgg tgcgccatgc gtggaaggag caaaagtggt
     4621 ttgtgatgta gtaggacacg taagaggtga aaaagtaatc gtttttcaca aaaaacgtag
     4681 aaaaggatat agaaaacgca ccggacaccg tcaggatttc actgaattat tgatcaaaga
     4741 aattgtagct taattttaaa aattcactaa aaatggcaca taaaaaagga gtaggaagct
     4801 caaaaaatgg tcgtgaatcg gaaagcaagc gacttggagt aaaaatttat ggcggacagt
     4861 ttgccaaagc cggtaatatc attgttcgtc aaagaggtac ggttcatcat cccggaaaaa
     4921 atatcggaat ggggaaagac catactttgt atgctctggt agatggaaaa gttgaattcc
     4981 gcaagaaagt cggtaatcgc tcttatgttt ccatcaatcc cgaaatgaat tgaaaaattt
     5041 cagtctcttt caatgcaaga gacttgtaaa cacatgataa tctcctgata atttacccaa
     5101 cggtaaaatt gtaggagatt tttttattaa atatttctca aacctcaatt ttttggccga
     5161 agtttttcgg tcgtctttta actgctaaaa taaaaaattg tttttataat gctgacactt
     5221 aaatatattg ccgaaaattc cgaagatgtt ctgaaacgac tgaaaaagaa acattttgat
     5281 ggaaaagagt taatttcaaa agttctggag cttaacgccg aaagaaaaaa aactcaatat
     5341 gacgccgatt ccatcgcagc cgaaataaat aaaatatcca aagaaattgg gattctttat
     5401 aaacaaggta aaacatcgga tgccgagact ttgaagcaaa aaacccttga gctgaaagaa
     5461 aatattaaac ttcttgaaga aaaacaaaac cagattgaag cccaactgaa cgaaattctg
     5521 gtgcaaattc ccaatttacc ccacgaaagc gtacctgaag gaaaacttgc agaagacaat
     5581 caggtggaac gtaccggagg aaaaattccc tcattgccaa cagatgcctt gcctcattgg
     5641 gacttggcaa aaaaatacga cattatagat tttgaaaccg gcgttaaaat tacgggagcc
     5701 ggatttcccc tttataaggg aaaatgtgcc cgacttcaac gcgctcttat caactttttc
     5761 cttgacaatg ctcgcgaagc cggttatatg gaagttcagc caccttatgt tgttaacgaa
     5821 gcttcggctt atgccaccgg acagcttccc gataaggaag gtcaaatgta tttctgtcag
     5881 gaagacaatc tttatttgat tccgaccgca gaagttcccg ttaccaacat ttacagaggt
     5941 atgattctca acgaaaacga attgccgata aaaaatgtgg cttattctgc atgttttcga
     6001 cgcgaagccg gttcgtacgg aaaagatgtc agaggattaa atcgtctcca tcaatttgat
     6061 aaggtggaaa ttgttcaaat tcaacatccc gaaaaatctt atgaaacact ttacgaaatg
     6121 gttcactatg tggaaggatt ggttgaaaag ctcgaattac cctggcgaac actccgactt
     6181 tgcgggggag acatgagttt tacttccgct cttaccttcg acatcgaagt ttattcggct
     6241 gcccaacaac gttggctgga agttagttca gtttccaact ttgaaagtta ccaggctaac
     6301 cgactgaagt gccgttacag aagcgcggat aagaaaattg aactttgtca caccctcaac
     6361 ggcagtgctt tggctttgcc tcgaattgtg gctgccttgc tggaaaataa tcaaactccc
     6421 gaaggtattc gcattccaaa agctttgatt ccgtacacag gatttgagat aattgattga
     6481 tttttcctat aaaacaaaag ctatatttca attttttaag attggatttg tttcaaggca
     6541 aatccaatct tttttttgta attgtgtttc cactaaaaat gctgagccaa aaaaaccgct
     6601 atgaatcaga aaaaatacga acaggtattt tgtaaaaaat gaataaattc caaattttta
     6661 tgtaagtttg tagaaaaatc attgtatgga atctatttct tcattcgctg aactgatagc
     6721 ccatttaaaa tttttaaaca acagaaaacg cgttgcagta gcaaatgcag tagatccaca
     6781 tacattggaa gcggtactaa gagcttcgga taacggtttt gttgaagctt tcctgattgg
     6841 cgacttggcc atgatagaat ctccacactt ttttgaacac aaactaactc cttatgtgca
     6901 cattatcgat attccggaag tggaagaagc tacacgcgaa gcggtaagaa tggtcagaac
     6961 aggcgaagcc gacatattaa tgaaaggatt ggtaaacacc gacgtgattt tgcgggcaat
     7021 tctgaataaa gaaaacggtt tggtagaaaa aggaaaaatt attacctaca atgcagcctt
     7081 agaaataccc gattaccaaa aactgctgtt ttttaccgat ccggcagtca tcccctctcc
     7141 tactgtagaa caacggactg ccatgataaa atacgtaacg gaaacagctt acaaatttgg
     7201 aataacccga ccaaaaatag ctttaatcca tgcaacggaa aaagccaatc cgaaaattca
     7261 atacatgcag gattatctcg acataatgga gaaatggcga atgggtgaat ttggcgatgt
     7321 tattatggat ggcccgctcg atatttttct tgctttagac cctgaaagag gaagtatcaa
     7381 aaaagtatct tcacccattc atggcgatgc agatattttg attttcccaa gttttgaatg
     7441 tgcaaatgct ttctataaag gattaatggt ttttgcaaag gccgaaatgg caggtcttct
     7501 tcagggaacg caaaaaccgg tagtgctgac ctcgagaagc gaaagcgtca actccaaata
     7561 ttacagtttg gctatggctt gtgtcatggg gtaaggacag acttgtcgga atgcaacaac
     7621 tcgaagattt tttttgcagc tcggcttgcg gctccgggtt ctcccaatat ctcagaaact
     7681 tttttatact catttttcat attttgcctg taatgcgcat ctgtaagaat tctatttaat
     7741 tcatttttca cattttctac cgtaaacaaa tgagcataca gctccatcac cacttccttt
     7801 ccggcaatca gattgactaa agaaatatat ttcactttaa taaaaagttt tttcaaccaa
     7861 taagccactt tcccaaaggc aacatgataa acaaccactt gcggagtgcc gattaaagca
     7921 gtttcgagag tagctgttcc tgaattgaca atggcaactt cggcatgaaa aagcaactca
     7981 taagtttgac caaaaaccac agcaactgaa gtatctttta aaacagaaaa ataaaaatct
     8041 ttctcaacac ccggtgctcc ggcaaccaca ggctgataat cgggaaactg cattgcagcc
     8101 tcaaccatgc gcggtaaaca ggatttgatt tcctgtcgcc gacttccggc caatagtgct
     8161 atcacaggtt tatcgaccaa attattggat tgacggaaag tttggaaatt tccccgataa
     8221 gctttcttaa actcttccac cgcatccact gtaggattac caacatattc gaccggataa
     8281 cgatgttttg cataaaactc cgtttcgaaa ggaaaaatcg taaacattct atctacgtaa
     8341 cgtctgattt ttttaattct aaactctttc catgcccata tttttggaga aatataataa
     8401 tatacgggaa tattcaattt cttttttgca aattcggcca ttctgaggtt gaaactcgga
     8461 taatcgacca gtatcagcac atcaggctga aatttgagaa gcgcctgctt gccatctttg
     8521 atatttacag aaataaccga aaggtttttc aacacagcaa taaatcccat aaaagccatt
     8581 tgccgatagt gtcttatcat ttctccacct tcggtccaca ttaaatcacc gcccaaaaag
     8641 caaaattcgg cttgcagatc ctgctttttc agttcgtgca tcaaatgcga cgcatgcaaa
     8701 tcgcctgatg cttcacccgc aatcaaaaaa tatttcatga aaatataaaa tcaataattt
     8761 tagctatgta tacaaataat cggacaatta cagcataaaa agacttgcaa ttaaaaaagg
     8821 caatccgccc acaatggtgc ccgaagtaag acgaaaagca tcatttttat aaaaaacaaa
     8881 agccatcagc aaatcgggaa caatggacaa cagcagcaac ttccccaaca aaacgcctgg
     8941 ataaagacgt ttcaaaattt caaaaaaaga tatatcgaca ggatagaagc ttgttatata
     9001 gagccaaacg aaaacgcagg gcagtatcaa acccactaaa aagccaatat aaaaatggtt
     9061 gagacgtttc acggcataag aatttacaaa ttccattccc tgatcaagtc cattgcatca
     9121 taagctgtca aatcaatttt atcgggaaca atggaaataa aaccatgact gatagcccat
     9181 tcgtcggtat cctcggcatc gggttccata ttttcaaaat atccggtaag ccaataataa
     9241 gccctgccgg cgggatcggt tcttgcagca aactcctttg tccagtaccc cttgcattga
     9301 cgcgcaacac gcacaccgtg aatttctccg cagggagcat tcacattcag acaaacactt
     9361 tctggaagac ctttttccat cacaatttgt gcaattttca atacgtgagg ttcaaaaaaa
     9421 ctaaaatcag gatcgggaaa atggctggcc aacgaaaacc cgatggaagg aattccgttc
     9481 tcacaacctt caaaaacagc acccatagtg cccgaatata tgacattgat ggcagaattg
     9541 gagccatgat tgattcctga aaccaataaa tcgggtttac gattcagaat ttcattcagt
     9601 gcaattttta cacaatcggt tggcgttccg gtacaggaat atcgaaccat tccttcctct
     9661 tcttctattt ttctgtaacg aataggctgc gtaaccgtca aagcacttga ctgagccgaa
     9721 cgcggtatat cgggagccac aaccactaca tctcccagct gcatcattaa tttcgttaaa
     9781 atttttatcc cttttgcatc tattccatca tcatttgaaa tcaaaatcaa atttttattc
     9841 atgcttcctc attaattttt ctaacaacaa aattttaaaa atcgacaata aaaaatattc
     9901 tttcaaaatc agcgcttact tgtccgattt ggaatttttc cataccatta ttcccaaaaa
     9961 caatcccccc aaaaacaaga aatatcccac atcacgcata tccaacactc ctctactcat
    10021 agacttgtaa tgagcgttta ttccaagatt ttgaacaaaa tcaatcacag ctccggaaga
    10081 gaaaaaactt cccaataagt cgaaaccgta aaagaaaata aaacacagca acactgacac
    10141 aacaaacgaa ataatttgat tgtttgaaag cgatgacgaa aaaattccaa tggctatgta
    10201 aacacccgaa agaaaaatca accctataaa cgagccccag aaagctccgg catcaatatt
    10261 tcccactggt tcggccaaaa aataaaccga gaaataatac aaaacggttg gaaataaagc
    10321 aattaaaacc aaaacccaac cggccagaaa ttttcccaaa accagttctg tgatgctcaa
    10381 aggtttggtt atgagtattt cccaagttcc cgattttttt tcttcggcaa aagatcgcat
    10441 ggtaatggca ggacacaaaa acaagaaaag ccatggcgcc agataaaaca atccatccac
    10501 attggcataa cccgattcaa taatattaaa ttcgccggga ataacccaca aaaataatcc
    10561 tgtcaaggcc aaaaaaatgc caataaccaa atatcctgta gcactgctga aaaaacttcc
    10621 gagttctttt ttgaaaatag aaatcatacc aaccggcgtt tttgtttaaa ttatttactg
    10681 ataaagactg caataattcg ctcgattaaa aaatttgtta actttacgct ttcaaaggta
    10741 accaaaaatt gtcaaaggaa aaattacatt tatgggcaga aataaaaaaa aattcctgtt
    10801 attgccaatg tttttcgcaa tctactcctt tggctttgcg caaacgttta aaaattattc
    10861 tctgagtgca gatttcattt acggtgaaat tctcaagcac aacaagcatc tcgaaaatct
    10921 ggtcaaagga ccgctcaaag gcgaagaatt ttcaatagaa tggcaaacaa tgggagaaaa
    10981 agcgtggcat cagtatctta attttccttc cgttggaatt tccacagctt ttttggattt
    11041 cagtcatccc gacactttgg ggtatgctgt agccctatat ccatatttga aattcccgat
    11101 tgttcgtcag caacatttta atatggcttt caaagccggc ggagggctga gttatgttac
    11161 aaaaacattc tacaatgcta cagcttatca tcctgatggc agcgtatatc tcaacaaaag
    11221 caatgccgct atcggatcac atgtgaatgt ctatcttaca gccaatctca acatggaaat
    11281 tcctttgaca aaaggatgga gcattacttc caactgggga tggaatcata cttccaacgg
    11341 aagcattgtt caaccaaatt caggaatcaa tatgttggat atgtatttgg gaattaaata
    11401 ttttccaaat tacgagcgtt atatcccacc taaacctcaa ccattacctg acctgccgca
    11461 taaaatcgaa attgaaatgg cttttaccgg cggcatacga caactttatt acaaagacga
    11521 caaaacctat cccatcggca ctttcaatat agcagcatac cgaaccatga ccaattttta
    11581 cagaatggga tggggcgcag atttgtttta cgatggcgtt tttaatggta aatcagcttt
    11641 caaaagaact tatctcacta ccgacgagtt aaaatacaaa cttagaattg gcgcttcttt
    11701 tcgacacgag cttttagttg gcagactcac aacaggtttc cattttggtt tgtaccttta
    11761 taatccggtg aaaaatctcg aaccttacga agaagcaaaa aacggaactt taaacaaacc
    11821 tttgatttac aaatataata ttgaaaagga agatggttgg ttttacacaa aagctgtatt
    11881 aaaatatgct ttggacgaac atttcctcct atctttggga ttgaaaaccc atttgcaaaa
    11941 agcggaattt attgatgcgg gaataggata taaattttaa aatgttctcc aaccatttct
    12001 attcattctc gtaatatagc aatgcatcca tcatgagcgg caattcttct tcccgaatac
    12061 caaacgaaat caacctgtcc ttatcttcat taaacaaatc ctgaaaaact tcccatttat
    12121 tctgcaacat ttctaaagcc ttgcgataag cattggctgc tgcagacaac tgacgtcggc
    12181 accagctaat atgaccggaa agcaaataat cgtaagcatt gggttcagcc aaaataattt
    12241 tttctgaata ataatcggct ttatccagat ttccgtcaaa aaaattgcat tttgcaatcg
    12301 ctttccacac ttttatgtcg tccacatttt cggcatccag tttataataa attgccagcg
    12361 catctttaaa ttttccgagt tcaaaataac attgagcgat atgtatttgc gtactgtact
    12421 gaccgggttt cagaaaatcg gcatgttgat aaaatgccaa cgctttttca taatttccca
    12481 gcaaccgata acaaagcgca atttttcgaa tagtccagcc atcgtccggt tgaatcatat
    12541 cggctttcaa ataggcatcc agtgcctttt gcaattgcga agtctgctga tacgcataac
    12601 ccattttctg atacaacgca gcggaaggct gactctccga ctgaattttt tcataaagct
    12661 ccagtgcctg caaataatac cctttagaaa aataatattc cgctacactt tctgaaaaac
    12721 cgggaataga agacaaaata tcaaacagcc aggtgcgatg taaagtcaga gaataggaaa
    12781 acatatcttc aaaatcttcc ttgtgaggct gaagtttaaa gaaacggaac aaatcctgaa
    12841 tgtactgttt ggaaatgttt tttgtaattg tttcgggcga aagtatcgac atttcttctt
    12901 ccatttcgtc catttgatcc gattccattt taaaagaatg tttaagcata tttcgttggc
    12961 tttcgggcat ttgtaacaca ctcaggcaaa atgaatattt gtctgaattg cacatgacat
    13021 tgctgttgac aaaagccgac aaaatggttt tttcatccga ctcgaacaac tgattgactg
    13081 ccgaattttc agtatcgaaa ggcaagaacc aatgagaaaa ttgagagaaa aatgtaaaat
    13141 ttttcagcat ggaaaaagta ctcatgtaaa catccgctcc ttccaattgt aaatcgctca
    13201 attctttgag tttatccgac aagccacttt catcgaaaat ttcctgccat tccggatttg
    13261 cttctgccca ttcatcggaa ttcaaaaagt tttcagcatc cattttgtct ctcaacaaag
    13321 gattgatttt catcatttca ggcaaaattt cttccctgag tttccgcgaa attttttctg
    13381 tttcggtagt cccaataatt tgaataatga tattctgaaa attttcggca atacggtttt
    13441 gatcgactaa aagaaccaaa cgattacgaa tggaagggaa ataaggcaga taaatattgt
    13501 atttggccag tataaaagtt aacccgacta atgcccgctg aacaactttt tgatcggcgt
    13561 tttcgcaact gtcaaaaaga agcattattt tttgttcgtc aaattttctc cacaaattca
    13621 acgttacggc cgaaaccatc aaggattttt ccacccagta tttatcatcg gcttcgagca
    13681 atttcttgta caaattgttt tcttcttcgg tatagcgagt tgccagccaa aacacgccaa
    13741 aaagttcatc cagcgcatct tcaaaattgg atcgcattct gctcaattca attttgtact
    13801 gttccttatt ttcggaaagc aaagccgact gcttgtggaa atactgcaaa gcattgaaca
    13861 gttcattgac tgtctgatat ttacgagtgt atggaaaaaa tcttttctgt gaatattcga
    13921 aatttgaaga atttttcatc atcagctctt cgcgaacgtc agcaagcagt atcaaaagtt
    13981 ttgcgaccaa cttactgtaa accgatttcc tttcgggatc ttgcacaccc ttgagataat
    14041 aatcgagtaa aaatttgtaa ttcctttcca attcgttaaa acgatccgaa aattcgccgt
    14101 actgcaaatc gtcaatcatc atttttatcc tgtcgaatgc aggtttgagc cttgacgccg
    14161 acacaaatcg caaaatagcc ttataagtag tatcaatttg agttgcattc ataaataaaa
    14221 acaatggtca attaaatatc actttttcaa tttggagaaa gaatcacaaa gcgaaaaaca
    14281 acaaaaatta tcccatttta cttatttttc tgagtttatc ttcatcaata atcttaattt
    14341 ttcttccatc cacagcaatg atgtgctcgt tggtaaaatt cgataaagtg cgtatagcat
    14401 tggatgtggt catattcgac aaattcgcca aatcctcacg cgacaaatag atattgacgg
    14461 tagccccatc ttcttccact ccgtatcgtt ttttcagaaa cagcaaagct tctgccaaac
    14521 gaccacgaat gtgcttctgt gtcaaattga cagttcgtgc atcggaaact gccaggtcgg
    14581 aagccaattc ttcaagaaaa cgatagcaaa ctgcaatatt gtgtctgaga atggacaaaa
    14641 acaaatcggc cttgagcata taaacggttg aggcttcaaa agccgctaca ttggtgctgt
    14701 ttggctcatt ggctataatg gcacgataac cgaaattttc gcccggtttc aacatccgaa
    14761 taatttgcgt tcggctacca ataccatcct tgtaaacctt tgctttaccc gccgccaaaa
    14821 tcatcatgtg ggtagaaatt tcaccttcgc tttggatcag ttcgtttttc ttgaaatgat
    14881 gaaccgtgta gttgtttctt acaaattgcc tttcgtcggg cgtcagcaca tcccaaacca
    14941 tgaaaacttc ttcaatgggt ttcagttcat aagcaggttt tttggtcatt gcttcaaatg
    15001 aaaaaatatg tttgacaaca ttgttataca gcacaaattt aattattttt ttaaaataaa
    15061 ttccaaaacg taaaataaaa atatttctct attccgaata aaaattctaa cggggaaaaa
    15121 ttaaaaaatt tctgaattgt aaacatttga aactacctgt aaaatgtaga ggaaaacatt
    15181 gaataaaact tttaacaaaa agctatgaga aaagaaaaat ttattacttt tgccatttga
    15241 aatccataaa aaccaatcgc aatacatgaa aagagcaata ttcgtttctc tcctcagttt
    15301 catattattc ccaatattat cacaaaattt cacattcagc gattcggcaa aagtaagttt
    15361 gctcacttgt tctcccggga aaaaagtgta tgaaaaattc ggacatactg ctattcgtgt
    15421 caacgacccg attaccggct acgatgtggt tttcaatttt ggcgtattcg atttcaacac
    15481 caaaaacttc tacctgaaat ttattaaggg agataccgat tatcagcttg ccgtatatga
    15541 taccaaaagc tttttggaag aatataaaag tagaaattcg atggtttggg aacaaaaact
    15601 gaaccttact ttgaacgaaa aaagaaatct gcttcactct cttttaataa attatgaacc
    15661 cgaaaacaaa ttctaccgct acaattttgt ttatgacaat tgctctaccc ggccaagaga
    15721 taaaattgta caatcagtta atggaaagat tgaatatcat ttatataacg agcccgtaac
    15781 tttcagagaa atgatagctc aatacaccgg agtaaacagt tggatgcaat ttggtcttga
    15841 tctaatcttc gggcgtgatg cagacaagag cgtttcgcga aatgtatcga tgtttttacc
    15901 tgaaaatctg atgtacgaat ttcaggcagc tcacataata gccgcagatg gtgcaaaacg
    15961 ccacctggta gctgaaaata agatattgat tcccaaacag gaagaaaaat attctaaacc
    16021 tttgcctata tttcagcctg tatttgtttg tacttttctt ttaattttgg gaattatcat
    16081 aactattttc aacaataaaa aaagtttccg tttgttcgat agcctacttt atatagtaag
    16141 cggccttggt ggaattattg tcttttatct cgcctttttc tcatcgcatc cgcttgtaaa
    16201 aaacaatttt aatcttctgt ggctgaatcc gctgaatttt gctctgggca ttctcctttg
    16261 gtttcacaaa atgaaaccgt atgtgtttta ttatcaagtt ttaaactgtt tattgatttt
    16321 tctggctttg gtagcttatg ctgctggact tcaggttttc catccggcca gttttccgct
    16381 tatcctgttg cttttgctgc gaacagtgcg atggacagcc acaacaaaaa aagatttttc
    16441 gctaaagaaa aaaaataatc gggactatta caccaatagc aacgggcttc accaccactc
    16501 aaggaaaaat tgagataaaa tttctttata aaaaatcatt tgaccaatga ctctccattc
    16561 taatagaaat tttcgctttg tcggcattat tccggcccgc tttgcttcca ctcgttttcc
    16621 gggaaaaccg ctggcagata ttggtggaaa acccatgatt caacgagtgt atgaacaggc
    16681 acgcaaggta ttgcaaaccg tttatgtggc taccgacgac catcggattt atgattgcgt
    16741 taacaatttt agaggaaaag tacttatgac ttctgccgca gccaaaagtg gaacagatcg
    16801 gtgttatgaa gctgtaagtc agctacagga aaattttgat gccgttatta acattcaggg
    16861 agatgaaccc tttattcaac cagaacaaat cgttacgctt caacagtgtt ttgaagatga
    16921 acaaacacaa attgctacac ttgccaaaat agttcaccaa accgaagaat acgattacat
    16981 actgaatccc aacagaccta aaatagtact caataaaaac aatgaagccc tgtatttcag
    17041 caggcaagtt attccatacc aacgaaatac tgccaacgag cattggattt cgaatcatca
    17101 gtattttgtt catgtgggaa tttatgcgta tcgaacagag atattgaaag aactctgttc
    17161 acttcccgtt tcatcgctcg aaaaagcaga atcattggag caacttcgct ggctcgaaaa
    17221 tggataccga ataaaagttg gcttcacaac agccgaatct tatggcatcg atacaccgga
    17281 agatttggaa aaagtcagaa aaatgttttt gggtaaataa tcaccttcct tttttttttc
    17341 aaatgtaagg gaaagctctt cattacgaaa ttttctgata aattggataa gtactttacg
    17401 aaaacccatc ctattctcgc acgattttat cgtgtgggtg gtcttaaagc cgatagatag
    17461 caatacaatt gaaaatgcct tagggaaaag ataggttgaa ataattttaa ttagctacac
    17521 aatgcaatca tgcggcaacc aagtaaaaaa ttatgatcag aaaacagaat tatatatatc
    17581 atttttccat tgtggcaaca gttgggtaaa aaatcgctta ataaattgta accaattgat
    17641 taaaaactac aaattcaata tgacaaacgg ctcaatacta ttttaaattc attcaaaaat
    17701 ctgcctttca ttcaatgccc gctgatattt tcgagcattg atcaaatgct cagcatgtgt
    17761 ggtggcgaaa ttatgttctc cattcaacgt ttctttggca cacatataaa gataggaatg
    17821 atgcgtatag tttaaaacgg catcaatcac ctgaggcgaa ggcaatcgaa tgggaccggg
    17881 tggcaaacct tgaaatttgt aggtgttgaa gggcgaattg atttgcaaat gttgaccttt
    17941 gattcgtttc aaagaaaaat ctttccatgc aaattttatg gtaggatcgg cttgcaaggg
    18001 cattttattt tttaacctat tgatataaac tcctgcaata ataggctgtt catacgattt
    18061 gttggattct tcttctacaa ttgatgccaa aatactgact tctattggag tcagcggtat
    18121 ggaagcagct ttctgccggc gttcttcgtt ccaaaatttt aaatactctc tgtacattcg
    18181 atcgaaaagt ttttcgggtt tgatattcca gaaaacttca taagtatccg gtataaatat
    18241 cacaatcgaa ttatcgggat tcaaagcgta ttgcgacaaa aattgagaat tgttcagcaa
    18301 ttttaaaaaa gtgagtgaat cggtcattag ttgagacgac aatcgaccgg ccaattgctc
    18361 ttttgttcgt atattgttga atgtcaatcg caatggaacc tgtctcccac tccttaagtt
    18421 tcgaattaat gcaaaattgt tcattccttt ttttaacgca tagcgccccg aacggatatt
    18481 tttatcgtaa tcgaaaattt tggccgttaa ccgcaaagaa taactgtccc acacgcgtga
    18541 ttcatttttg ataagtttca cgacatcttc aaaactcgta ttctcgggaa tgcaaacaaa
    18601 agtttctgtt tctttttttg ggaaaatatt gggcgcaaaa aaaacgtagg tcaaataaac
    18661 aaccacaatt actggaaccg caagcgctgc aaacaaaatt ttcctgactt tctttttcat
    18721 aaaaaatgcg tttggatttg agttgcaaaa gtaaatgttt ttcttaaaaa aacacttatc
    18781 cgttctaaaa tgtaattcat taaaaatcaa tttgaattgt taaattcttt ttcccctaaa
    18841 attatcccga acaaaggttc agtaaaaatg actttgataa tcgaaaactc tttcttatct
    18901 ttgcattaca aaagcagaca caaggaagtg tgggtgagtg gctgaaacca tcagtttgct
    18961 aaactgacgt acgggtttcc gtaccacgag ttcgaatctc gtcgcttccg ctttatattg
    19021 ccggatactt ttcaccagtt ttcggctttt ttttaagaca acacacacaa catcaatccg
    19081 ataaatttta tcgtctctca aattttaaaa ttactactat ggaaaaaaca aaaaaattta
    19141 ttcttaccga aaaggaactt ccaacccatt ggtacaacat tgtggcagaa atgcagaaca
    19201 agcccatgcc aattctgaat cctgctacca aacagcccat gaaagaagaa gaattgtacc
    19261 cacttttcgc aaaagaattg tcgcatcagg aattaaatca gaccgatgca tggattgaaa
    19321 tacccgaaga agtacgcgag aaatataaaa tttatcgacc tactccgctg gtaagagctt
    19381 atgatttgga aaaagcgctc gacacacccg cacacattta ttttaaaaac gaaagtgtca
    19441 gtcctgttgg ctcccacaaa ttaaacacag ctttgccaca agcttattat tgtaaacagg
    19501 aaggcgttac ccacttgact accgagaccg gagccggtca atggggaaca gcgttggctt
    19561 ttgctgccaa aactttcggt ttggaattgt ttgtttacat ggtaaaaatc agttacgaac
    19621 aaaaacctta tcgtcgctca ctgatgcaaa cttggggcgc tcaggtaatt gcttctccaa
    19681 gcatgtccac caatgcagga cgaaaaattc tgtccgaaca tcccaactat ctcggaagtt
    19741 tgggaactgc catttctgaa gccatagaac ttgccatgaa tacgccaaac tgcaaatata
    19801 cgcttggcag tgttttgaat catgtctcac tgcaccaaac cgtaatagga ctggaagcag
    19861 aaaagcagat ggaaatggca ggcgaatatc ctgatatagt tattggctgt tttggtggcg
    19921 gttccaattt ctcaggaata tctttcccat ttttacgtca taatattaaa gatggtaaaa
    19981 aaacacgttt tatcgctgcc gaaccggctt cttgtccgaa attgacacgt ggtgtttttg
    20041 aatatgactt tggagatgaa gttggttaca ctccactttt gcctatgttt accttaggac
    20101 ataattttgc tccggctcac attcacgctg gtggtctgcg ttatcacggt gccggcacta
    20161 ttgtcagcca attgaaaaaa gacaacttgg tcgaagcagt tgatattgat caattggatt
    20221 ctttcaaagc cggcgtactt ttcgcacaaa ccgaaggaat tgttccggcg ccggaatcgg
    20281 ctcacgcaat tgcagtggct attcatgagg ctctgaaagc caaagaggaa ggaacaccaa
    20341 aaactatttt gtttaatctt tccggacacg gaatggttga tatgactgct tatgaccagt
    20401 attttgctgg cgacttaacc aattattccc tttctgaaaa cgacattgcc gaaaatataa
    20461 aaaatctgga acatttggca aaataaacaa atctgaaaat taatacgaaa aacaggacag
    20521 tgaaaaattt cacaggtcct gtttttttat aaccaaaagg taagaattta tctttctttc
    20581 atcaaaaaac gaccaatacg gcgcgtacgc tgcataagca aaacttctcg atgatttgta
    20641 acttcctgtg caatttcatc cgaaattcct ctgacagtca gcaattccaa attttcgttg
    20701 taacttacat caaactcatt cgacagttcg tccaaagcag ccggcaaata tcgactgttg
    20761 ccaatggcta ccgaaatgct gatggctgag ctttcaatca tcgaaactta tagccggtgt
    20821 ttattgatca tggaaaaaac ttttggcaaa ttttcttcca gcacaaaaga aaaatctatc
    20881 ggacgaatgg ttaccaacac ttgatttttc aacaaactgt aaaacggaat accggccggt
    20941 ttttcgacat gagctttaat aacagaaccc ttttcagagg gattcccgaa aggacgaaca
    21001 taaagcggaa tatttttatt ttgcaaaggt ttaatcgttt tgggatgaat cacctgagcc
    21061 ccgctgtaag ccaattccac cgcttcggaa taagtcaact cgggaacaag ctttgcctga
    21121 ggataaagac gtggatcggc attcaaaata cccggaacat ctttcca
//
LOCUS       DAOSCH010000023        20781 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1818_length_20781_cov_5.816607, whole genome shotgun sequence.
ACCESSION   DAOSCH010000023 DAOSCH010000000
VERSION     DAOSCH010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20781
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1818_length_20781_cov_5.816607"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            280..6168
                     /locus_tag="PLZ54_03220"
     CDS             280..6168
                     /locus_tag="PLZ54_03220"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOJ65978.1"
                     /translation="MKKLSFILLAVVFVVVAFAAFQQPTVSFDERWKKVEELAEKQLP
                     ESALKEVDAILKDAKKQNNSPEIIKATLYKMRFTLEKDPDQAPQLIKEFETFATSTSD
                     TAEKSLLYSMTAELYAQYFQQKAYSIRQRTEISDYVPENIDEWTKNIFFDKVSQLLAA
                     SLEPAEMLQKTDIHKFETILEKGDSSHRVQPTLFDFLAYRRIDILQSLEGAQSIKNPL
                     KSEDLFSDLSHFVSLLLDTTYSKSVENQVLQTYQQLLSFHLKDNNVPALLYADLQRLT
                     YLKENSQLSNSDELYLSALARLSEQYANNENVVEVMAAMAQYYLDKEYELEENMNFAK
                     KAYAICSEGIRRFPDYPRIALLKNIQQQITQKSVSLRHSDVVRSKSQLSLYISTKNVE
                     DLQMQIYRVNASSLDYFSFTKNPRNSGKDFPDRTLWQTSEIKIKPDTNFVAVDTVLKI
                     PSGTFGIYEVQLMIKGDTSSEVARSSYVVSDLSFMVRQNEPKKTALYVLNSETGWQQK
                     EVKVSLYEQKWTGNFYQMNLLQTAVTDKNGFCTFPYNNQGTKWIFLEKGEDRYFSSSV
                     NTYYWERDTNKEKPVQLEFFTDRAVYRPGQTVYFKGIAYYADAKRQEVAKSLKFDVSL
                     IDTHYQKIASKTFTTNKFGSFAGEFVLPADGLNGTFRLESGNFSKTIWVEEYKRPTFE
                     VKIQKPTDEVKFGEKVTLTGNVKAYSGYNIADAKVNFRVVRRPHWYCSWIRSEEKAIS
                     NGETKTDANGNFTLFFIPEKSKENFSFRKDAYYVYTVYAEVTDTKGETQQGQQFVAVG
                     DKSLFILADVPEKWNKKEKADFPLRLETINGEKVDGTVQFTVYRFENSEDFATNEKWA
                     EMEEEGEEEISMKSAKKIMEGTFNTKDGKLKLDVNKWESGGYRIVFSTLDQSGKKVEL
                     GKNFILYGKNDKRPSIQTMTWLPVEEITCEVGETAEVVFGSSADNVAVLYEMMQGNTV
                     LESKWIRMNNEIKTFPIPFKAEYGDGITVMFTFMKKGLFFTESVIIRRKIAEKKLTPT
                     LTVFRNKLLPGEKAEWTVNIPEIKQIKKQAELLMDMYDASLDVIRPNEWTFQPIFRGN
                     FMESPRWDAPYNRIISDNIDIPVKYIPVEEYRLDRLNWFGLDLMAGQKYNGPFVVRGL
                     KSLSATSEFREISAMNEKFEEETVVAFSVVKTVKTDLTNVIMQPETSPVIRKNFNETA
                     FFYPQLYTDSAGDVKFSFTAPESLTRWNIKMLAHTPDLYFGQADAQAITQQDLMIQMN
                     LPRFVRCSDTLILSCIITNLSEKEISPVAHFELLDAANEQPVAVFEDSKVQLSPNETK
                     TVRWQLKDFSSHELLICKVMATAGSFSDGEQNYLPVLPDKILVTESLPMTVRGHQTKT
                     FQFESLQKKFSQVETKNLTLEFAAQPVWYAIQALPTLAEPENQNAVDYFAAYYVSTLA
                     AYIVQSYPEIEKIFRRWEKSETGKNALLSNLEKNSELKNFLLKETPWLMAAQDEMEQK
                     NRLALLFDLNMQQDKSQQYLDKLLKLQLSTGAFTWFEGMTENRYITQLVLLNMKRLHE
                     KTGLPMDKRLESAMTLALNYLDKEIVNDFEQLKKNRKNFEKENCISPLQLQYLYLRSS
                     YSEIPVPDFSQEAVKFYRHQAEKFWTDFSLYGKSQAAIVAYRDGKMALARNILKSLKE
                     NALKSEEMGMYWANNKAGYFWNERPVAVQTAIMEAFSEIGADTSDLEEMKTWLLKQKQ
                     TQRWDSPISTVDAVFALLTIGNNWLQNNDKVIIKIGDKELQPSSIEAGSGYFKQSLPV
                     EQITSASSRITVSKNSDGIGWGAMYWQYLQKMDQVQSTVGALNISKKLFVEKVTSAGR
                     TLVPIEQVKISLGDKVVTRLVVTTDRNLEFVALKDIRAAGLEPVNQISGCQWKQGVCY
                     YQTIKDASAEFFFSSLPKGTYVFEYALWANNVGTFNGGTASIQCQYAPEFVGYAAGQQ
                     VVVQ"
     gene            complement(6234..7619)
                     /locus_tag="PLZ54_03225"
     CDS             complement(6234..7619)
                     /locus_tag="PLZ54_03225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ65979.1"
                     /translation="MKIQINIKLKENRWLLMAIVIGSFFNSFMSSSLNIAMPVIDNEY
                     HVGLAKVAWISESFLLASAVGLMLAGWLADQFGRRKTFLFGNLIFGFISLFIYFVPGF
                     YPAVMLRFIQGLGSALIMASAPALIASAFDPSRRGFVLGIQTTFTYVGLSLAPLLGGA
                     LTQIFGWRSLFLFNFIFGLAALSIMFFGVKREWKAETRNKFDVGGYVIFLLAIVALVL
                     ALDWMNKFSIIMLLFSLMAGYVFIRYELKKEMPFISIAFLKSNKYFTNSNFAALINYA
                     TTFAISYVLSLYLQNVRGMTPVGAGLILCFQPLMMALFSAYAGHLSDRHNPGKIASYG
                     MAMIALALFIIGITLSEKLPLAIIAGLLAFLGFGFALFSSPNTNAVMSSADAGSYGFA
                     SAFLAFNRLFGQFISMALASAFILLSNHTRLATVEEQALLMASKIIFIFFGLLSCLGV
                     YLSYQRIRMKK"
     gene            7931..10396
                     /locus_tag="PLZ54_03230"
     CDS             7931..10396
                     /locus_tag="PLZ54_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOJ65980.1"
                     /translation="MIYPKHFEQKIDFITIRQLLKDKCVSTLGIEKVDEIRFSSNFDE
                     IFHWLSETNEMLKVLTTEVQELPVNHFYDLRSSLSKIRIEGLYLTETEIFQLYQTLEN
                     VLTITNYISQLNPQLYPCLHQLTGNVNVFKPIIQRVDAILSKFGKIKDQASPQLTQIR
                     KNMTEVQNNISKSIQEILRKAQTNGYVEKDTTPALREGRLVIPVPQAFKRKINGIIHD
                     ESASGKTVYVEPAEIVEMNNRLRELEGEERRETIRILTLFADFVRPYLPDIQESQNFL
                     GKIDFLRAKALLAIDIHAIQPQLENHCLIDWHKAIHPLLFLSFSKQNKQVVPLDIQLN
                     EKQRILIISGPNAGGKSVCLKTTALLQYMLQCGLLIPLHESSRTGIFEHLFIDIGDEQ
                     SIEDDLSTYSSHLLNMKFFIRYSNDKTLLLIDEFGSGTEPQLGGAIAEAVLDRINHNK
                     AFGVITTHYTNLKQFAEETEGIVNGAMLFDRQKLQPLFQLQIGMPGSSFAIEIARKIG
                     LPEDIIDQASKKIGKDYLNYDRYVQDIIRDKRYWQNKREQIKLKEKQMEETIRRYETE
                     LTELKRQRTEIIQKAKEEAQNILEETNAKIENTIRQIKEAKAEKEKTKEIRKELENFK
                     QSVNQSIDNRFSVNPAAVSTKKKLVKKVTPKEEIHKKDVQEEKISVGSNVRLKNQGTT
                     GTVLEINGNEAIVAFGQMKSTVPFSKLEPLSRTKLKKENQNKKNIHVNTIEAVRQRKL
                     EFKTEIDVRGMRSNEALQAITYYIDDAIMVGVPSVRILHGTGTGALRQIIREYLRTIP
                     AVRNFHDEHIQFGGSGITVVELE"
     gene            10604..10765
                     /locus_tag="PLZ54_03235"
     CDS             10604..10765
                     /locus_tag="PLZ54_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65981.1"
                     /translation="MKKVLLFCAVIFAFAACAPKESKEASTTEDSVVVVDTVVVDSMV
                     VDTTAAPMK"
     gene            complement(10841..11794)
                     /locus_tag="PLZ54_03240"
     CDS             complement(10841..11794)
                     /locus_tag="PLZ54_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOJ65982.1"
                     /translation="MEKPYVIGIDMGGTNTVFGIVDVRGNVISKSAIKTAVHDDVNLY
                     VNDIYHELMKLIDAEGGIDQIRGIGVGAPNGNYYSGTIEFAPNLPWKGVIPFAKLLNE
                     KFGVPVALTNDANAAAIGEMTYGIARGMKDFIMITLGTGVGSGIVIDGKLVYGHDGFA
                     GELGHTTSIRNGRLCGCGKNGCLEAYSSATGVARTAREILETTNQDSILRNIPLADIT
                     SKDVYDAAKEGDQVAIDIFNFTGKILGEAFADFVAFSSPEAIILFGGLSKAGEFILNP
                     IIENMEKNLLPIWKGKIKILFSELKESDAAVLGASALAWEL"
     gene            complement(11817..13172)
                     /locus_tag="PLZ54_03245"
     CDS             complement(11817..13172)
                     /locus_tag="PLZ54_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007411075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ65983.1"
                     /translation="MNAEKKSGNLVAIITMFFLFAMISFVTNLAAPIGVIWKNQPAFE
                     GSNFLGMLGNMMNFLAYLFMGIPAGKLLSKIGYKKTALVAIAVGFLGVLTQYLSGIIG
                     VGAELFSLPANFFVYLLGAFIAGFSVCMLNTVVNPMLNLLGGGGNKGNQLIQIGATFN
                     SLAATITPMFVGALIGDITKERILSGEVTINNVNPVLYIALGVFALAFLILLFIPIAD
                     PDENRKKRSEIKYEHSPWNFRHFVLGSIAIFIYVGVEVGIPGTLNFFLADTTIKGASL
                     DPQKAAAIAGFVAGTYWFLMLIGRFLGSLVGGKVSSKTMLTFVSALGIALVLLAILLP
                     KDITTSMPVFTGSSFSMVQVPLIALFLVLCGLCTSIMWGSIFNLAVEGLGKYTAAASG
                     IFMMMVVGGGILPLLQNWIADISTYQASYIVILVGLLYILFYALIGSKNVNKDIPVEV
                     E"
     gene            complement(13360..15528)
                     /locus_tag="PLZ54_03250"
     CDS             complement(13360..15528)
                     /locus_tag="PLZ54_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOJ65984.1"
                     /translation="MKRIFLAFVLAFAVQFSFADLLDDIVDGKFNVNNIMVPRSMKDG
                     ENYTLMKDNAVLKYNYKTGQLVDTVFSIHRLKDAPFKEINGYEFSPNEAKLLVYNNVK
                     KRYRRTFTADYYVYDIKRNEIQPLSDNGSQEVPLFSPDSRYIAFGRNNNLYLKKLDFN
                     TESAITKDGEPGKIINGLPDWLYEEEFEATRYFVWSPDSKLLAFVKFDETEVPQFSFQ
                     LYENKNSMDDLMLYPSLVSFKYPKAGQNISKVSVCVYEERTKAIRTVQLSNVGEDFYI
                     PRIKWTNSLDQLAVFVLNRNQNQLKMFLANPKSTLSTLILQENDKYYIDYQNIDACQF
                     TSDNQYFIFLSEKDGFRHVYQHRINGTPVKQLTKGNFDVTQVYGFDEKNQTLYYQSAE
                     SSPLQRDIYSVSLKGVKKKLTNGNGFHNAIFNSTFSFFVDNFSNLQTPDKLILYSNNG
                     NFIREMQNSDGILENWNTLHLPKKEFFSFTTSENISLNGWMLKPLNFDPNKKYPLLLI
                     QYSGPNSQMVLDKWDVDWEYYLAENNMLVVCVDGRGTGARGVEFRKCTYEQLGVLEAK
                     DQTEAARFFSKQDYIDSQRIGIWGWSYGGFLTLMAMSSGENVFKAGIAVAPVTDWRFY
                     NAAYTERYMRRPQENFKGYDLASPIMKADRLKGELLIIHGTADDNVHAQNTLMYVKRL
                     VEADKQYNMQLFTDKNHSILGKQTRRYLYRSMSNFLFKNL"
     gene            15694..16515
                     /locus_tag="PLZ54_03255"
     CDS             15694..16515
                     /locus_tag="PLZ54_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ65985.1"
                     /translation="MNDDNITNHEQNINKTGICLSGGGALGFAHIGVLQALEENGIFP
                     VQIAGSSMGAIVGTLYAAGFTPAQMLQMIREDKLYKITKLMSIHPMSLKSGLSTHKML
                     RDLIKELIPHNSFELLKKKMSVCVSNLNKAEWEIIESGKELDKWVAASASIPGVFEAI
                     QVKKEYYVDGGLLNNMPAQALKENCSTIIGVDVIPHLNEVQLKKPIDTLTYSIRIIQH
                     KNAQKGRELCQYLIEPKAIRKFHEFSFDAYQQIYEEGYQCTHTFIENNPAINNGI"
     gene            complement(16596..18170)
                     /locus_tag="PLZ54_03260"
     CDS             complement(16596..18170)
                     /locus_tag="PLZ54_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOJ65986.1"
                     /translation="MNSELVVDVKPSEISIALLEDKRLVELAQETREEQFAVGNIYLG
                     KVKKIMPGLNAAFVDVGYEKDAFIHYLDLGPNFNTLSQFTLQALSDPKKAPSMQKVKM
                     LPEIEKTGFINDLLKPGQQILVQVAKEPISTKGPRLTAEISLAGRFLVLIPFNDKVLI
                     SQKIKSENERVRLKQLIQSIKPKGFGVIVRTVAEDKKVAELINELNVLVKRWDEAVSK
                     LQQAKGVTLISEELGRTVGIIRDLFNPSFKNIYVNDPNVFAEVQDYIEWIAPEQKNIV
                     KLYKDETPIFDHFGITKQIKSSFGKTVSFKNGAYLIIEHTEALHVIDVNSGNRAKTGN
                     GQEANALEVNLAAADEIARQLRLRDMGGIIVVDFIDLHESDNRQKVYDRMKEMMANDR
                     AKHNILPLSKFGLMQITRQRVRPDTHVDIEEACPTCFGTGKIKPSILFTDLLESKIDY
                     LVNTLKVKEFTLYVHPYVEAYISKGMIPLKNKWKIKFGRGCKVRASQELGFLQYKFID
                     NKGMELNLQEEIEKIS"
     gene            complement(18405..18677)
                     /locus_tag="PLZ54_03265"
     CDS             complement(18405..18677)
                     /locus_tag="PLZ54_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ65987.1"
                     /translation="MTKAEIVNEIAKKTGIDKATVLASVEAFMDAIKDSLSKNENVYL
                     RGFGSFVVKTRAQKTARNISKNTTIIIPPRNIPSFKPAKKFMSVVK"
     gene            19117..19779
                     /gene="ung"
                     /locus_tag="PLZ54_03270"
     CDS             19117..19779
                     /gene="ung"
                     /locus_tag="PLZ54_03270"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOJ65988.1"
                     /translation="MDVKIESSWKEVLQSEFEKEYFKQLTDFVRKEYKTKKIFPPGSL
                     IFNAFNLCPYDKVKVVIIGQDPYHGPGQANGLCFSVNEGVDFPPSLVNIYKEIENEFA
                     KPMPKNGDLSSWAKQGVLLLNAILTVQAHLAGSHQGKGWETFTDAVIRILAEKKEHLV
                     FLLWGSYAQQKGLYIDTNKHLVLKSPHPSPLSAYRGFIGNRHFQLANQYLKEHGMSEI
                     EW"
     gene            19977..20615
                     /locus_tag="PLZ54_03275"
     CDS             19977..20615
                     /locus_tag="PLZ54_03275"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOJ65989.1"
                     /translation="MTVIHFFVLKYSTVEFIGLFISLFGLFIRAYAVGHTPANTSGRN
                     TKEQLADELNTTGIYSMVRHPLYLGNFFMWLGAAVLTKNGWFIVAFILLYWIYYERIM
                     FAEEQFLRRKFGSVYTDWANKTPAFIPSFKKYIPSKYPFSWKKVLKKEKNGLLAVFVL
                     LFVFHNINYSFLTNHIRIDLNWIAWGTFAAGILYLVLKFLKKKTSILNETGR"
BASE COUNT         6773 a         3977 c         3900 g         6131 t
ORIGIN      
        1 acaaatatat gaattttcaa aaacaacatt tttaaaaact ttcgtacggc agtgataatt
       61 ttccatttct caaccataag gtcgctccta cagagctttc aagtcacaca gggacaacct
      121 tttggtagaa ctgagtagag ccgttccatg caacatctct acaaaaacaa ttttccctgt
      181 cgaattttgc tcaaaaaatt tcttcctgcc gattttggta ctatatttgt cttccatttc
      241 gcttcacaaa taaaaatatc cgtttaaaat ttcttcctca tgaaaaagct ttcttttata
      301 ttgcttgccg tagtttttgt tgtggtggct tttgctgcct ttcagcaacc gactgtttcc
      361 tttgacgaac gctggaaaaa agtggaagaa ctggccgaga aacagttgcc cgaatcggca
      421 ctgaaagaag tggatgccat tttgaaagac gcaaaaaaac aaaacaattc tccggaaatt
      481 atcaaagcta ctctttacaa aatgcgtttc acgctggaaa aagatcccga tcaggcacca
      541 caactcatca aagaatttga aacttttgca accagtactt ccgacacagc cgaaaaatcg
      601 ttgctttatt ctatgacggc agagctctat gctcaatact ttcagcaaaa agcctattcc
      661 attcgtcaac gaacggaaat ttccgattat gttccggaga atatcgacga atggaccaaa
      721 aatattttct tcgataaagt cagtcagttg ctggctgctt cattggaacc ggcagaaatg
      781 cttcaaaaaa ccgatattca taaatttgaa accattctcg agaaaggaga tagcagccat
      841 cgggtacagc ccacactttt tgattttttg gcctatcgcc gtatcgacat tttgcaatcg
      901 ttggaaggtg cacaatccat aaaaaatccg ttaaaatccg aagacctgtt ttctgaccta
      961 tctcattttg tttcgttatt attggataca acctattcca aatccgttga aaatcaggtg
     1021 ttgcaaacct atcagcaatt gctttctttt cacttgaagg ataataatgt gccggcactt
     1081 ttgtatgccg atcttcagcg gttaacatat ctcaaagaaa attcccagtt gtcgaatagc
     1141 gatgagctgt atttgtcggc tctggcacgt ttatcggaac aatatgcaaa caacgaaaat
     1201 gtagtggaag ttatggctgc aatggcacag tattatctcg acaaagaata tgaattggaa
     1261 gaaaatatga attttgcgaa aaaggcttac gctatttgtt ccgaaggaat tcgccgtttt
     1321 cctgattatc cgcgtattgc attgttgaaa aatatccagc agcaaataac gcagaagtct
     1381 gtttctttgc ggcattctga tgttgttcga tcaaaatcac aactctcatt gtatatttct
     1441 acaaaaaacg tggaagattt acaaatgcag atttaccgcg tcaatgcttc atcgctcgat
     1501 tatttttcat ttaccaaaaa tccgcgaaat tcaggaaaag attttcctga ccgtaccttg
     1561 tggcaaactt cggaaataaa aattaagccg gatacaaatt ttgtagccgt cgatactgtt
     1621 ttaaaaattc cttcgggaac ttttggaatt tacgaagttc agttgatgat aaaaggcgat
     1681 acttccagtg aagttgccag aagttcttat gtggtttccg atttaagttt tatggtacgc
     1741 cagaacgagc cgaaaaagac agcattatat gttttaaata gcgaaaccgg atggcaacaa
     1801 aaagaagtga aagtgagcct ttacgaacaa aaatggacag gcaattttta tcagatgaac
     1861 cttttacaaa ctgctgttac cgacaaaaac ggtttttgca catttcctta caacaatcag
     1921 ggtacgaaat ggatatttct cgaaaaagga gaagatcgat attttagttc ttctgtaaat
     1981 acttattatt gggaaagaga tacaaataaa gaaaaaccgg ttcagttgga atttttcacc
     2041 gaccgtgcag tgtatcgtcc cggacaaaca gtctatttta aaggaattgc ctattatgcc
     2101 gatgcgaagc gtcaggaagt ggcaaaatcg ctaaaatttg atgtttcgct tattgatacc
     2161 cattatcaga aaattgcttc aaaaacattc actaccaata aatttggttc atttgcagga
     2221 gaatttgtct tgcctgccga tggattgaat ggaactttcc ggcttgagtc gggaaatttt
     2281 tccaaaacta tttgggtgga ggaatacaaa cgtcccactt ttgaagtaaa aattcaaaaa
     2341 ccgaccgatg aagtaaagtt tggggaaaaa gttacgctaa ccggcaatgt gaaggcttat
     2401 tcaggttata atattgcgga tgcaaaagta aattttcgtg tagttcgacg tccacactgg
     2461 tattgcagtt ggatcagaag cgaagaaaaa gcaatttcga atggagaaac aaagactgat
     2521 gcaaacggta attttacact tttttttatt cccgaaaaat caaaggaaaa cttttctttc
     2581 agaaaagatg cctattatgt ttacacagtt tatgccgaag ttaccgatac caaaggcgaa
     2641 acacaacaag gtcagcagtt tgtagcggtt ggcgacaagt cgttgtttat tttggccgat
     2701 gttcccgaaa aatggaacaa gaaagaaaaa gccgattttc cgctgcggct tgaaaccata
     2761 aacggtgaaa aggtggatgg tacggtacaa tttaccgttt atcgatttga aaattcggaa
     2821 gattttgcaa ccaatgaaaa atgggcagaa atggaagaag aaggtgaaga agagatttcc
     2881 atgaagtcag caaaaaagat aatggaagga actttcaata ccaaagatgg taagttaaaa
     2941 ttagatgtaa ataaatggga atcgggtggt tatcgtatag ttttttctac gcttgatcag
     3001 tcgggcaaaa aagtggaatt gggtaaaaac tttattcttt acggaaaaaa cgacaaacgt
     3061 ccgtctattc aaaccatgac ctggttgcct gtggaagaaa taacctgtga agtaggtgaa
     3121 acagctgaag ttgtttttgg ttcgtcggct gataatgttg ccgtgcttta tgaaatgatg
     3181 cagggaaata ccgttttgga aagcaaatgg atcaggatga acaacgaaat caaaactttt
     3241 ccgattccat tcaaagctga atatggagat ggcatcacgg tgatgtttac ctttatgaaa
     3301 aaaggactgt ttttcactga aagtgtaatc attcgccgaa agatagcgga gaagaagttg
     3361 acgccaactt taaccgtttt tcgcaataaa cttttgccgg gtgaaaaagc ggaatggaca
     3421 gtcaatattc ctgaaataaa acagattaaa aaacaggcag aattgttgat ggacatgtac
     3481 gatgcttcgc tggatgtcat tcggccgaat gaatggacat ttcagcctat ctttcgtgga
     3541 aattttatgg aaagtccgcg ctgggatgca ccttacaaca ggataatttc cgataatatt
     3601 gatattccgg ttaaatatat tccggtagaa gaatatcggt tggatcggtt gaactggttt
     3661 ggtctggact tgatggcagg tcaaaagtat aatggtcctt ttgttgtaag aggcttaaaa
     3721 agtttaagtg caacatctga atttagagag atttcggcca tgaatgagaa attcgaggaa
     3781 gagactgttg ttgctttttc agtagtaaaa acagtaaaaa ccgatttgac gaatgtaatc
     3841 atgcaacctg aaacttctcc agtcattcgg aaaaatttca atgaaacggc atttttctat
     3901 ccgcaacttt ataccgacag tgcaggcgat gtgaaatttt ctttcactgc accggaaagt
     3961 ttgacccgat ggaacattaa aatgctggct catacgcccg atttatattt cggacaagct
     4021 gatgcacagg ccatcactca gcaagattta atgattcaga tgaatttgcc tcgttttgtt
     4081 cgttgctcgg acacgttgat attgagttgc attattacca acctttcgga aaaagaaatt
     4141 tccccggtgg cacatttcga actgttggat gcagcaaatg aacagccggt tgcagttttt
     4201 gaagacagca aagtacagtt atcccccaac gaaacaaaaa ccgtgcggtg gcaattaaaa
     4261 gatttttctt cgcatgaact gttgatttgt aaggtgatgg caacagcagg cagtttcagc
     4321 gatggagagc agaattatct tccggttttg cccgataaga ttttggtaac agaaagttta
     4381 cccatgacag tacgcggtca tcaaaccaaa acttttcaat ttgaaagttt acagaaaaaa
     4441 ttctcacagg tggagactaa aaatctgacg ctggaatttg ctgctcaacc ggtttggtac
     4501 gccattcagg ctttgcctac tcttgccgag cccgaaaacc aaaacgctgt tgattatttt
     4561 gctgcctatt atgtttctac gctggcagcc tatattgttc aatcttatcc cgaaattgaa
     4621 aagatatttc gacgttggga aaagtcggaa acagggaaaa atgcactttt atcgaatctg
     4681 gagaaaaatt ccgaactgaa aaacttttta ctgaaggaaa caccctggtt gatggctgct
     4741 caggatgaaa tggagcagaa aaatcgattg gctttacttt tcgatttgaa tatgcagcaa
     4801 gacaaatcgc aacaatatct cgacaaattg ttgaaacttc aactttcgac cggagctttc
     4861 acttggtttg aaggcatgac ggagaatcgt tacatcacgc agcttgtttt gctcaatatg
     4921 aaaaggttac acgaaaaaac cggtttgcca atggataaaa ggttggagtc ggccatgacg
     4981 ctggctttga attatcttga caaagaaatt gtcaatgatt ttgagcaact gaagaaaaac
     5041 cggaaaaatt ttgaaaagga aaactgtatc agtccgcttc aacttcagta tttgtatctc
     5101 cgttcgagct attcggaaat tcccgttccc gatttttcac aggaggcggt aaaattttat
     5161 cggcatcagg cggaaaaatt ctggacggat ttctcacttt atggcaaatc gcaggctgcc
     5221 attgttgctt atcgtgatgg aaaaatggct ttggctcgca acatactgaa atctttgaaa
     5281 gaaaatgcac tcaaatcgga agaaatgggt atgtattggg ctaacaataa agccggatat
     5341 ttttggaacg aacgtcctgt tgctgttcaa acagctatta tggaagcttt ctcggaaatt
     5401 ggagctgaca cttccgacct ggaagaaatg aaaacctggt tgctcaaaca aaaacaaacg
     5461 caacgatggg attcgcccat ttcaacagtt gatgctgttt ttgccctgct taccatagga
     5521 aataactggt tgcagaacaa cgataaagtt attatcaaaa ttggcgataa agaacttcag
     5581 ccttcatcca tcgaagccgg gagcggatat ttcaagcaat cgttgcctgt ggagcaaata
     5641 acatctgctt caagtcgcat cacggtcagc aaaaactcgg atggaattgg ttggggagcc
     5701 atgtattggc aatatttaca aaaaatggat caggtgcaga gtacggtcgg tgcattgaat
     5761 atttccaaaa aattgtttgt ggaaaaagtt acttccgctg gtcgaacgct tgttccaatc
     5821 gaacaggtta agatttcgct gggagacaag gttgttacgc ggctggtggt tactaccgac
     5881 agaaatctcg aatttgttgc tttgaaggac attcgtgcgg cgggtctcga accggtgaat
     5941 caaatttcgg gctgtcagtg gaaacagggt gtttgttatt atcagaccat aaaagatgct
     6001 tcggcagaat ttttcttttc gtctttgcca aaaggaacct atgtgtttga atatgccctt
     6061 tgggctaata acgtcggaac tttcaacggc ggaacagctt ccattcagtg tcagtatgca
     6121 ccggaatttg tcggctatgc agcaggacaa caggttgttg ttcaataact tttcgggaaa
     6181 aatttgatga tttgagtgtg taaagaaatt cgcatgattt tgttttggta tttctatttt
     6241 ttcatgcgaa ttctctgata agaaagataa actcccagac aactaagcaa gccaaaaaag
     6301 atgaaaatta tttttgaggc cattagtaaa gcctgttcct caacggttgc taagcgggta
     6361 tgattactca acaaaataaa tgccgatgcc aatgccattg aaatgaactg tccgaacagc
     6421 cggttgaaag ccaaaaaagc cgaagcaaaa ccataagaac ccgcatctgc cgagctcatc
     6481 acggcattgg tgttgggtga agaaaaaaga gcaaatccaa atcccaaaaa tgccaacaat
     6541 ccggcgataa ttgccagcgg aagtttttcg ctcagagtaa ttccaatgat aaacaatgcc
     6601 aaagcgatca tagccatccc gtaagatgct attttgccgg gattgtgtct gtccgacaaa
     6661 tgacctgcat aggctgaaaa caaagccatc atcagtggtt gaaagcataa aataagaccg
     6721 gcgccgacag gtgtcattcc gcgtacattt tgcaggtaca aactcagcac ataactaatg
     6781 gcaaatgtgg tggcgtaatt gattaaagct gcgaaattgg aattggtaaa atatttgttc
     6841 gatttcagaa aagcgattga aataaatggc atttctttct tcagttcata gcgaataaaa
     6901 acataacctg ccataagact gaataacagc atgataattg aaaatttgtt catccagtca
     6961 agtgcaagta ccaatgcaac tatggccaag agaaaaataa catatccgcc aacatcaaat
     7021 ttgtttcttg tttcggcttt ccattctctt ttcacaccaa aaaacataat ggacaaagcc
     7081 gccagtccaa aaataaagtt gaaaagaaac agacttctcc acccgaaaat ttgagtcaaa
     7141 gcgccaccca gaagcggagc cagtgaaagt ccgacgtaag taaaggtggt ttgaattccc
     7201 agtacaaatc ctcttctgga agggtcgaaa gcagaagcaa tcagggcagg agcggaggcc
     7261 attatcaatg cgctccccaa tccttgtata aatcgcaaca ttacagccgg gtaaaaaccg
     7321 ggaacaaagt aaatgaaaag tgaaataaaa ccgaaaatca aatttccaaa aagaaaagtt
     7381 ttgcgtcgcc caaactgatc ggccagccaa ccggccaaca ttaatccgac ggcagaagcc
     7441 aacagaaaag attccgatat ccatgctact ttggccagtc ctacatggta ttcgttgtcg
     7501 ataacgggca tggcgatatt cagcgatgag gacatgaaac tgttaaaaaa tgaacctatc
     7561 acaatggcca tcaataacca tctgttttcc ttcaatttta tattgatttg tattttcata
     7621 ccgtttgctt atacaattta ctaaaatact ttttaaaacg gcttaatgaa atatttttca
     7681 taattttgtc ttcccacaga ggggttcagt ctgcaaacaa cataatatga aaaatcaatc
     7741 attcgattta tttactttac aaaagtaagg tgataaacat gggtggttta aagaaaaagg
     7801 ctagcaaatt tataatattt tttggacaaa actgttttta aaaaaataga gtttatctta
     7861 tctttctcaa tacgcagcaa accaagtttt taatagctat ttgattttaa aaaatttaat
     7921 tacatgctca atgatttatc ctaagcactt cgagcagaaa atcgacttca ttaccattcg
     7981 gcaactgctg aaagataaat gtgtcagcac gttgggaatc gaaaaagttg atgaaattcg
     8041 tttttcttcc aactttgatg aaatctttca ttggctgtcg gaaaccaatg aaatgttgaa
     8101 agtgttgacc actgaagtgc aagaactgcc tgtaaatcat ttttacgatt tgcgttcttc
     8161 tctttccaaa attcgtatcg aaggtcttta tcttaccgaa acagaaatat ttcagttgta
     8221 tcagacgctg gaaaacgtgc tgaccatcac caattatatt tcacaactga atccgcagct
     8281 ttatccttgt cttcatcaac tgaccggcaa cgtaaatgtt ttcaaaccca tcatacaacg
     8341 tgtcgacgcc attctttcca aatttggaaa aatcaaggat caggcatctc ctcagttgac
     8401 gcagatacgc aaaaacatga ccgaagttca gaacaatatc tcaaaatcta ttcaggaaat
     8461 tttgcgtaaa gcgcagacta acgggtatgt agagaaagac accactcccg ctctacgtga
     8521 agggaggttg gtaatacctg ttccacaggc atttaaaaga aaaatcaacg gtattatcca
     8581 tgatgaatcg gcaagcggaa aaaccgttta tgtggaaccg gccgaaatag tggaaatgaa
     8641 taaccggttg cgtgaactag aaggagaaga acgccgcgaa acaatccgaa ttttgacctt
     8701 atttgccgat tttgtacgtc cctatttgcc tgacattcaa gaatcacaga attttttggg
     8761 aaaaatagat tttttgcgtg ccaaagcatt actggctatt gatatccatg ccatacaacc
     8821 gcaattggaa aatcattgcc tcatcgactg gcataaagcc attcatcccc tgctttttct
     8881 ttcattctcc aaacaaaaca aacaagttgt tccgctggat attcagctga atgagaaaca
     8941 gcgtattcta attatttcag gtcccaatgc aggaggaaaa tccgtttgtc tgaaaactac
     9001 agcgttgttg caatacatgc tgcaatgcgg cttgctgatc ccgctacatg aaagcagtag
     9061 aaccggaatt tttgaacatc tctttatcga tattggcgac gaacaatcca ttgaagacga
     9121 cttgtccacc tacagttcac atttgttgaa catgaaattt tttattcgct acagcaacga
     9181 caaaacatta ctgctgattg atgaattcgg ttccggaacc gaacctcaat tgggtggcgc
     9241 aatagctgaa gccgtactgg accgtatcaa tcacaacaaa gctttcggcg taatcaccac
     9301 acattacacc aatttaaaac aatttgctga agaaacggaa ggaatagtca acggagcgat
     9361 gctcttcgac cgacaaaaac ttcagccgct tttccagctt caaattggta tgccgggtag
     9421 ctcgtttgcc attgaaatag cgagaaaaat aggattgccc gaagatatta tcgatcaggc
     9481 atcaaaaaaa atcggaaaag attatctcaa ttatgaccgt tatgttcagg acattattcg
     9541 cgacaagcgt tattggcaaa acaaaagaga acaaatcaaa ctgaaagaaa aacaaatgga
     9601 ggaaactatc cgccgttacg aaaccgaatt gactgaactg aaacggcagc ggacggaaat
     9661 catacaaaaa gctaaagaag aagcccaaaa tattctcgaa gagacaaatg ccaaaattga
     9721 aaataccatt cgacaaataa aagaagcaaa agcagaaaaa gaaaaaacca aagaaatcag
     9781 gaaagaactg gaaaatttca aacaaagtgt caatcagtct attgataatc gattcagcgt
     9841 aaatcccgct gctgtttcaa ctaaaaagaa acttgtaaaa aaagtaacac ccaaagaaga
     9901 aatccataaa aaagatgtac aggaagaaaa aatttctgtt ggcagcaacg tcaggctgaa
     9961 aaatcaaggt actacaggaa ctgttctcga aattaatgga aatgaggcta ttgtcgcttt
    10021 cgggcaaatg aaatctacgg tgccgttctc aaaactcgaa ccactgagcc gcaccaaact
    10081 taaaaaggaa aatcaaaaca aaaaaaatat ccatgtcaat accatcgaag ccgttcggca
    10141 acgcaaactc gagttcaaaa ccgaaatcga tgtacgcgga atgcgtagta acgaagcttt
    10201 gcaagccatt acctactata ttgacgacgc cattatggtt ggagtccctt cggtacgcat
    10261 tcttcatggt accggaacag gtgctttgcg ccaaattatt cgggagtatt tacgcactat
    10321 cccggctgta cgaaactttc acgacgaaca tatccaattc ggtggctcgg gtattacagt
    10381 agtggagttg gaataaaaat aaaatttctt ttaatttttt taggtaaaaa caagataaat
    10441 taaaacaaac agtatggaat taaaaaattg aatgctgaaa ttattctgaa aaaaaacgaa
    10501 aaaaaaaatt attttgataa caatatattt aaaatattca taactttgca gaaattttcg
    10561 tcatagaagt tcacatgtta aatttttaaa ttatttttga aaaatgaaaa aagtattgtt
    10621 gttttgtgct gttatttttg catttgcagc ttgtgctcct aaagaaagca aagaagcctc
    10681 aaccacagaa gattctgtag ttgttgttga tactgttgtt gttgacagta tggtggttga
    10741 cactaccgct gctccaatga agtaaaaatg ccgttttttt tttgtaatcg agtaggtgag
    10801 attttcattt tcttcctact cgattatttt ttaagttatt ttaaagttcc caagctaatg
    10861 cactggcacc caatacagct gcatccgatt ctttcagttc ggaaaacaag attttgattt
    10921 tacctttcca aataggaaga agattttttt ccatattttc tataatagga tttaaaataa
    10981 actctcccgc ttttgataaa ccaccaaaaa gaataatggc ttccggagaa ctgaaagcaa
    11041 caaaatcggc aaatgcttca cctaaaattt ttccggtaaa attaaaaatg tctattgcca
    11101 cctgatctcc ttccttagca gcatcataaa catcttttga agtaatatcg gcaagaggaa
    11161 tattacgcaa gatactgtct tgattggtag tttcaagaat ttcacgagct gtgcgtgcca
    11221 caccggtagc agaagaatag gcttccaaac aaccattttt tccacagcca cataaacgcc
    11281 cattgcggat ggaggttgta tgacccaatt cacctgcaaa tccgtcatga ccgtaaacca
    11341 gctttccgtc aataacaatt ccacttccaa cgccggttcc caatgtgatc atgataaaat
    11401 ctttcatgcc gcgagcaatt ccataagtca tttcgccgat ggctgcagca ttggcatcat
    11461 tagtcaaagc cactggaacg ccaaattttt cgttaagcag ttttgcaaaa ggaattactc
    11521 ctttccaggg caaattggga gcaaattcga tggtaccgga ataataattt ccattgggag
    11581 ctccaacccc aatccctctt atttgatcta taccgccttc ggcatcaatc aatttcatta
    11641 attcatggta aatatcattt acataaagat tgacatcatc gtgaactgca gtcttgatgg
    11701 cacttttaga aatcacattt ccacgaacgt ccacaatacc gaaaacggta ttggttccgc
    11761 ccatatctat tccgataaca taaggttttt ccataatacg ataattttat tgaaatttat
    11821 tccacttcaa cgggaatatc tttattaaca tttttagaac caattaaagc atagaacaaa
    11881 atatacagca atccaaccaa aattacaatg tagcttgcct gataagtaga aatgtcggca
    11941 atccagtttt gcagaagtgg taaaatgcca ccacctacta ccatcatcat aaatattcct
    12001 gaagcagcag ccgtatattt tccaagtcct tctactgcga ggttgaaaat actaccccac
    12061 ataatggaag tacacaagcc gcaaagaaca aggaataaag caatgagcgg tacttgcacc
    12121 atgctgaatg atgaaccggt aaatacaggc atggaagtag taatatcttt cggcaataaa
    12181 atggcaagca acaccaatgc aattcctaat gcggaaacaa atgtaagcat ggttttacta
    12241 gaaacttttc caccaaccaa actaccaaga aaacgtccaa taagcatgag aaaccaataa
    12301 gtgcctgcca caaaaccggc aatggcagcc gctttttgag gatctaaaga agctccttta
    12361 attgttgtat cggcaaggaa gaaatttaac gttccgggta tgcccacttc tactcctaca
    12421 taaataaata tggcaatgga tcccagtaca aaatggcgga aattccacgg tgaatgttcg
    12481 tatttgattt cggacctttt ctttctgttt tcatccgggt ctgcaatagg aataaacaaa
    12541 agaataagaa aggcaagtgc aaaaacgccc aaagcaatat aaagcacagg atttacgttg
    12601 ttaatggtaa cttcacccga caaaatgcgt tctttggtta tatcaccaat caaagctcca
    12661 acaaacatag gcgtaatggt tgcagccaaa gaattgaaag ttgctccgat ttgaatcaac
    12721 tgatttcctt tgttgccacc gcctccaagt aaattcaaca ttgggttgac aactgtgtta
    12781 agcatacaaa cagagaaacc ggctataaat gcacccaaca aataaacaaa gaaattggca
    12841 ggtagtgaga aaagttcagc tccaacacca ataataccgg acagatactg tgtcagaaca
    12901 cctaaaaaac ctactgcaat tgcaacaaga gccgtttttt tataaccaat ttttgaaagc
    12961 agtttaccgg ctggaatacc cataaacaga taagccaaaa agttcatcat atttccaagc
    13021 atcccaagga aattcgatcc ttcaaaagca ggctgatttt tccaaattac cccaatcggc
    13081 gcagcaagat tagtaacaaa cgaaatcatt gcaaacagga aaaacatggt gataatggcc
    13141 accagatttc cacttttttt ctctgcattc atattgttta gaattaataa agttaattgt
    13201 aatgaaaatg ttttttgaat taatttatat tgaccgacaa aaataccatt tttttgtcag
    13261 ttccttttaa cagaataaac aaaaaagctt tatccaaaca atgttttaca acatattttt
    13321 aaccaataaa aacagtttga ttttttatat ataatggaat tacaaattct taaacaaaaa
    13381 attactcatc gaacgataca aatacctacg cgtttgtttt cccaaaatgc tgtgattttt
    13441 gtccgtaaac agctgcatgt tatattgttt gtcagcttct accaatcgtt ttacatacat
    13501 cagggtattt tgtgcgtgaa cattatcatc ggccgtccca tgaatgatca gcaattcacc
    13561 ctttaatctg tctgctttca ttatgggcga agcaagatca tatcccttaa aattttcctg
    13621 aggacgacgc atgtatcttt cggtgtatgc agcattgtaa aatctccagt cggttaccgg
    13681 agctacagca atgcctgcct taaatacatt ttctccacta ctcatggcca ttaacgtaag
    13741 aaatccgccg taactccaac cccaaatacc gattctttgc gaatcaatat aatcttgctt
    13801 gctgaaaaaa cgggctgcct ctgtctggtc ttttgcttcg agaacgccta actgttcata
    13861 cgtacatttt ctgaattcta ctccgcgcgc tccagtgcca cgtccatcaa cgcaaaccac
    13921 caacatgtta ttttcagcca aataatattc ccagtccaca tcccatttgt ctaaaaccat
    13981 ttgcgaatta ggcccactat attgaatcaa caataatgga tattttttat tcggatcaaa
    14041 attcaacggt ttgagcatcc agccatttaa cgaaatattt tcagaagtcg taaagctgaa
    14101 aaattccttt ttgggcaaat gaagggtatt ccaattttcc aatatgccgt cagagttttg
    14161 catttcacga atgaaattgc cattgttact atacaaaata agtttatcgg gagtttgtag
    14221 attggaaaaa ttatcgacaa aaaagctgaa ggtcgaatta aaaatggcgt tgtgaaaacc
    14281 attcccattg gtaagtttct ttttaacgcc ttttaagctg acagaataaa tatctctttg
    14341 caaaggagaa ctttctgccg attgataata aagcgtttga tttttttcgt caaaaccata
    14401 aacttgcgtt acatcaaaat ttccttttgt cagttgtttg accggcgtgc cattgattct
    14461 atgctgataa acatgacgaa agccatcttt ctcactcaga aaaataaaat attgattgtc
    14521 ggaagtaaat tggcaagcat caatattttg ataatcaata taatatttat cgttttcctg
    14581 caatatcaga gtggacagag tagattttgg attggcaaga aacattttca actgattttg
    14641 attgcgattc aacacaaaaa ctgccaactg atccagtgaa ttggtccatt ttatcctcgg
    14701 aatataaaaa tcttctccaa cattactcaa ctgaacagtt ctgatcgctt ttgtacgttc
    14761 ctcataaacg caaacactta ctttggaaat attttgtcca gctttgggat atttaaaaga
    14821 aacaagtgat ggataaagca ttaaatcgtc catagagttt ttgttttcat acaattgaaa
    14881 tgaaaattga ggcacttcag tttcgtcaaa ttttacgaaa gccaataatt tgctgtcggg
    14941 gctccaaaca aaataacgcg ttgcttcaaa ttcttcttca tacagccagt caggcaaacc
    15001 attgataatt ttacccggtt caccatcttt tgttatagcc gattcggtat tgaaatcgag
    15061 ttttttcaaa taaagattgt tgttccttcc gaaagcaata tatctactgt cgggagaaaa
    15121 aagaggtact tcctgcgagc cgttatccga aagcggctga atttcgttcc ttttaatgtc
    15181 gtaaacataa taatcggctg tgaaagtacg cctgtatcgt ttttttacat tgttataaac
    15241 caatagtttg gcttcatttg gactgaattc ataaccatta atttccttga atggggcatc
    15301 tttcaagcga tgaatactaa aaacggtatc taccaattga ccggttttgt aattgtattt
    15361 tagcactgca ttatctttca tcagcgtata attctctcca tctttcatcg agcggggaac
    15421 catgatatta ttcacattaa acttgccatc aacaatatcg tccagcaagt cggcaaacga
    15481 aaattgcact gcaaatgcca aaacaaaagc taaaaaaata cgtttcataa aaaactttta
    15541 accgttaaat tacctattag ttgatttttt tcttgtaaat tcacacaaaa gtagcgtaat
    15601 tcatcagttt taaaaatttt aggtcaattt ttttgaatgg tttaacatta tacaagaaag
    15661 aacatccaca acaatacaaa aatacatata acaatgaacg atgacaacat cacaaaccat
    15721 gaacagaata ttaacaaaac aggcatttgt ttgagtggag gcggcgcttt aggctttgcc
    15781 catattggcg ttttacaggc actcgaagaa aatggcattt tccccgttca gattgcaggc
    15841 tcaagtatgg gagccattgt aggaacgctt tatgctgctg ggtttacacc ggcccaaatg
    15901 ctgcaaatga tcagagaaga caaattgtac aaaataacca aattaatgtc tatccatccc
    15961 atgtcgttga aatcgggact atccacccac aaaatgttgc gggatttgat aaaggaattg
    16021 atccctcaca acagtttcga attattaaag aaaaaaatgt ctgtctgcgt cagcaatttg
    16081 aataaagccg aatgggaaat aattgaaagc ggcaaggaac tggataaatg ggtagccgct
    16141 tcggcaagta ttccgggcgt atttgaagcc attcaggtaa aaaaagaata ttatgtggat
    16201 ggcggattat tgaacaacat gccggcacaa gctctcaaag agaattgtag tacgattatc
    16261 ggcgtagatg tgattcctca cctgaatgag gttcaattaa aaaaaccgat tgacaccttg
    16321 acttattcca tccgcatcat acaacataaa aacgcacaaa aagggagaga actttgccaa
    16381 tatttgattg aaccgaaagc gatccgaaaa tttcacgaat tcagttttga tgcttaccag
    16441 caaatttatg aagaaggcta ccaatgcact cacactttta tagaaaataa cccggcgata
    16501 aataatggaa tttagcatac cgaaataaaa aactgccttt agatggtaac atccaaaagg
    16561 cagttttctg ttagaatttt tttacaaatg tccaattacg aaattttttc tatttcttct
    16621 tgaagattca attccatccc tttattatcg ataaatttat actgcaggaa acccaattcc
    16681 tgtgaagctc tcactttaca tcctcgtccg aatttaattt tccatttgtt tttcaaagga
    16741 atcattcctt tggaaatata ggcttcaacg tatggatgta catagagagt aaattcttta
    16801 acttttaaag tattgaccaa ataatctatt ttgctttcca ataggtcagt aaataaaata
    16861 gaaggtttta ttttccctgt tccgaaacaa gtagggcaag cttcctcaat atcgacatgc
    16921 gtatcgggtc gaacacgttg acgtgttatt tgcatcagtc cgaacttgct gagaggcaaa
    16981 atattgtgtt tggctctgtc gtttgccatc atctctttca tccggtcata cactttctga
    17041 cgattgtcag attcatgcaa atcaatgaaa tccactacaa taattccgcc catatcgcgc
    17101 aaacggagct gtcgggctat ttcgtcggca gcagccaagt taacttccaa agcgttggct
    17161 tcctgtccgt ttcctgtctt tgcccgattg ccactgttca catcaataac gtgtaaagct
    17221 tccgtatgtt caataatcag ataagctcca tttttaaatg aaacggtttt accaaaagac
    17281 gattttattt gtttggtaat gccgaaatga tcaaatatcg gagtttcatc tttataaagc
    17341 tttacgatat ttttttgttc cggagcaatc cattcaatgt agtcctgcac ttctgcaaat
    17401 acattgggat cgttcacata aatgttttta aaactcgggt taaataaatc cctgataatt
    17461 cctacagtac gacccaattc ttccgaaata agagtaactc cctttgcttg ctgaagtttg
    17521 gaaacggctt catcccagcg ttttacgagc acattgagtt cattgatcag ttcggcaact
    17581 tttttgtctt ctgccactgt acgaacgata acgccaaacc ctttcggttt gatgctctga
    17641 ataagctgtt taagacggac acgctcattt tcagatttaa ttttctgaga aataagcacc
    17701 ttgtcgttaa agggaattaa caccaaaaat cgtccggcta aagaaatttc ggctgtcagc
    17761 cgcggtcctt tggtggagat gggttctttg gccacctgca ccagaatttg ttgacccggc
    17821 ttgagcaggt cgttgataaa acctgttttt tcaatttccg gaagcatttt aactttttgc
    17881 atcgagggcg cttttttcgg gtcgctgagc gcctgcaaag taaactgaga aagggtattg
    17941 aaatttggac ctaaatcaag ataatgaata aaagcatctt tttcgtaacc gacatcaaca
    18001 aaggctgcat ttaagcctgg cataattttt tttacttttc ctaagtaaat attcccgaca
    18061 gcgaattgtt cttctcgtgt ttcttgggca agttcaacca gacgtttatc ttcgagcagc
    18121 gcaatagaaa tttctgatgg ctttacatca actactaatt cgctattcac ttttttattg
    18181 attttggtta ttcgattttc aacaaaaaaa caaactcaaa gcctcaatga atgctttaaa
    18241 gtttgtttcc ttgaacttta atcatttaaa gacccatcga cctatttttt cttgtgtctg
    18301 ttctttctta gtcttttttt acgcttgtgc gtagacattt tgtgtctttt tctttttttc
    18361 ccgcttggca ttttttaaaa ttttaatttt tgaaatgaaa taatttactt cactacagac
    18421 ataaatttct ttgcaggttt gaaagaagga atgttgcgag gtggaatgat aatggttgtg
    18481 ttttttgaaa tgtttcgggc agttttctgt gctctggttt ttaccacaaa actaccaaaa
    18541 cctcttaaat atacattctc atttttagaa agagaatctt ttattgcatc cataaaagct
    18601 tcaacagatg ccaaaacagt agctttatcg atccctgttt ttttggcaat ttcatttaca
    18661 atttctgctt tagtcatttt tgctaatatt ttaaatatgt taataaatta agaaaactat
    18721 ttattgaatt gaaataggat tgcaaatata ttgcttttat tctgattaaa aaacatttgc
    18781 gctatttttt ttagctaaaa ttgatttttt taatgcaaga ctaaaatata aaatgaaatt
    18841 ttacatggaa gcaaacatta aaatttcttt attcaacgta aaatcaaatt gtttattatc
    18901 agtaaaatat aaacatcggc tttgaattaa tttctttttc tgaattgtcc aatgggaaaa
    18961 atttttaatc cattgaaaat aaactgctaa agaaaaagtt ataaattaaa aaaaacaaac
    19021 agatcattga actttcgtag ccgtataaat atttttttga tgtaaatttg caccggaaaa
    19081 ctttattttt cacagcgata taaaaaacct tttctaatgg acgtcaaaat agaatcgagc
    19141 tggaaggagg tgcttcaatc ggaatttgaa aaagagtatt ttaaacagct taccgacttt
    19201 gtacgaaagg aatataaaac aaaaaaaatt ttcccgccgg gctctcttat ttttaatgct
    19261 ttcaatctgt gtccgtatga taaagtgaaa gtagttatta taggtcagga tccgtatcat
    19321 ggtcccggtc aggctaacgg tttatgtttt tcggtaaacg agggcgtgga ttttccgcct
    19381 tcgctggtca atatttataa ggaaatagaa aatgaatttg ccaaaccaat gcccaaaaat
    19441 ggcgatttaa gctcttgggc aaaacagggc gtgttgctgc tcaatgccat tctcaccgtg
    19501 caggctcatt tggccggttc tcatcaagga aaaggttggg aaacatttac cgacgcagta
    19561 attcggatat tggccgaaaa aaaggaacat ttggtgtttt tactttgggg aagttatgcc
    19621 caacaaaaag gcctatatat cgacaccaat aaacatttgg tgctgaaatc gcctcatcct
    19681 tctcctcttt cggcttatcg gggatttatc gggaatcgac attttcaact cgccaatcaa
    19741 tacctgaaag agcatggtat gagcgaaata gaatggtaaa tcctctcaaa aaaagtaaat
    19801 caaaacaata acaattaaac ttttctataa ttaatcaact atacaaaaat catttaaata
    19861 atattttatg gcattgcagg aagaaatgga gaaacagggt aatttccttt tcaagtatcg
    19921 tggatattta cctttgatta ttgtagcagt agggcttgcg gtttttattg aaccacatga
    19981 cggtcataca tttttttgta ttgaaatatt cgaccgttga atttatcggt ttgtttattt
    20041 cacttttcgg acttttcata agagcttatg cagtgggaca tacgccggca aatacatcgg
    20101 gacgaaacac caaagaacag ttggcggatg aattgaatac caccggaatt tattctatgg
    20161 ttcgtcatcc actctatttg ggtaattttt tcatgtggct tggtgctgct gtcctgacga
    20221 aaaatggctg gtttatcgtg gctttcattc ttctttactg gatttattac gaaagaatca
    20281 tgtttgccga agaacagttt ctgcgtcgaa aatttggttc tgtttatacc gattgggcca
    20341 acaaaacgcc ggcttttatt ccaagcttta aaaaatacat tccttcaaaa tatcctttca
    20401 gctggaagaa agtactcaaa aaagaaaaaa acggcttgct ggctgtattt gtcctcctgt
    20461 ttgtatttca caatatcaat tattcttttc taacgaatca tatccgtatt gatttgaact
    20521 ggatagcttg gggaacattt gcagcaggca tcctttatct ggtattgaaa tttttgaaaa
    20581 agaaaacttc gatattgaac gaaacgggaa gataaatctg aaaaatattt tttacaaaaa
    20641 aaccggtgaa attttctacc ggtttttttt gtatgtatgg tattaagctt gcaggatgcg
    20701 agttccggta tgtgccgagt tgataagaaa cattagcgaa tggcaagggt gttgcgggtg
    20761 actgcaaatc tgaaagaagc c
//
LOCUS       DAOSCH010000024        20670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1832_length_20670_cov_5.567257, whole genome shotgun sequence.
ACCESSION   DAOSCH010000024 DAOSCH010000000
VERSION     DAOSCH010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20670
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1832_length_20670_cov_5.567257"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(524..2959)
                     /gene="lon"
                     /locus_tag="PLZ54_03280"
     CDS             complement(524..2959)
                     /gene="lon"
                     /locus_tag="PLZ54_03280"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOJ65990.1"
                     /translation="MKHSFDDFNIEELTQPSEAFPIISFEEEKNGDVSLESGDILPIL
                     PLRNMVLYPGVLLPVSVARPKSLKLVREAKESNHYIGVCTQKDKNIDDPTPDQLYSMG
                     TVAKVVRILEMNDNTTTVILEGKSRFFLGEVLTLKPYMKARINLAPEIIPSAEDGVFP
                     ALVATIKDMAAKIIANAGIMPPEAVFAIRNIDNPVFLINYVCVNFGLNVQEKQRLLEI
                     DDILDRGYQLLDLLNKEWQLLEIKISIENKAKEGIDQQQKQYFLQQQMKTIQEELGGA
                     SPEQDILDFREKAKQKKWNENVAALFEKELKKLERMSPHSPDYTTQLNYVETLLELPW
                     NEYTKDNFNLKNARKILEQDHFGMETVKERILEHLAVLKLKGDMKSPIICLYGPPGTG
                     KTSLGKSIARALNRKYVRISLGGLHDEAEIRGHRRTYIGALPGRIMQNIQKAQSANPV
                     FVLDEIDKVAADYKGDPSSALLEVLDPEQNTSFHDNYLDVDFDLSKVMFIATANNLNT
                     IPRPLLDRMELIEVSGYTQEEKVEIARRHLVPKELENHGLTKEQLILTKPVIEHLIEN
                     YTRESGVRELDRQIASLMRKIAKKVASNEPYNVQLTVDDLKTFLGVPRYTKEKYQGND
                     YAGVVTGLAWTQVGGEILYIESSLSKSKSPQLTLTGNLGEVMKESAVIALEYIKAHAE
                     QLGIDSEMFEKWNVHIHVPEGAIPKDGPSAGITMLTALVSAFTKRKVRKNLAMTGEIT
                     LRGKVLPVGGIKEKILAAKRAGITELVLCEENKKDVEEINASYLKGLHFHYVNDIMEV
                     INFALLDEKVRME"
     gene            complement(3393..3875)
                     /locus_tag="PLZ54_03285"
     CDS             complement(3393..3875)
                     /locus_tag="PLZ54_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOJ65991.1"
                     /translation="MRTFFIEDQVEIENIINACDICFVGISELDGSPYVIPMNFAFDH
                     NEVILHSAPEGKHQLLLQKDNRVCITFCSDRKLMYQHPDVACSYSMNSKSVLVKGKVI
                     FIENLEEKEQAMNLFMKKYTQRSDFKYSLPALRNVKVWKVKVESITAKAFGQNFKTQQ
                     "
     gene            4299..4463
                     /locus_tag="PLZ54_03290"
     CDS             4299..4463
                     /locus_tag="PLZ54_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65992.1"
                     /translation="MKKLFLLCAVVFAFVACGPKENKEAAATEDSVVVVDTVVAPVDT
                     AAVDTTVAPM"
     gene            4638..5489
                     /gene="hisG"
                     /locus_tag="PLZ54_03295"
     CDS             4638..5489
                     /gene="hisG"
                     /locus_tag="PLZ54_03295"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOJ65993.1"
                     /translation="MLRIAVQTKGRLFDDSMSLLNEAGIKIELGKRLLLIPAQNFPLE
                     VLFLRDDDIPESVADGVADIGIVGENEFAEKQKNVKLIRRLGFSKCRLSLAVPKEAEY
                     SDICWFEGKTIATSYPVILSNFLKEKGIRAEIHVITGSVEIAPSIGLADAIFDIVSSG
                     STLVTNHLKEVEVVMKSEALLIANPKLSEAQKKILDELLFRIEAVKMAEDKKYVLMNV
                     PTSQLEKIIEIIPGMKSPTVMPLAKEGWNSVHAVIAEKEFWDIIGKLKACGAEGILVL
                     PIEKMIL"
     gene            5504..6229
                     /locus_tag="PLZ54_03300"
     CDS             5504..6229
                     /locus_tag="PLZ54_03300"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOJ65994.1"
                     /translation="MNLCIDQGNSSIKMGIFDQSKLVKTFVYKNFDRKSVLSMLRNYP
                     VDRCIYSSVINKNASAVAFLKKNIAQVLLLTHETPLPIENKYQTPKTLGKDRLAAVVG
                     AGFLEPNRDVLVIDAGTAITFDFMDAKRNYWGGNIDVGLELRLMALHTFAKKLPLVKP
                     VQEVPLMASNTEMALAAGALNGIIFEIDGYINALKIEYPDLSTFLTGGSLIFFDKKLK
                     NSIFVEKNLVLIGLNYILQYNVQ"
     gene            6267..7469
                     /locus_tag="PLZ54_03305"
     CDS             6267..7469
                     /locus_tag="PLZ54_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65995.1"
                     /translation="MQDTSSQNNTNSPYTRFGFGEINDNHNSEQRAMGGVALGVRSVS
                     SINSINPASYSVVDSMTFMFDFGVSALRSQFKDPSGKSSNFTANLDYITIQFPLSKNI
                     ALSAGIQPYSFSGYNFYTTEILTIPNNSNTPDSLNVTKSFTGSGGFSEVYMGLSIDFL
                     QHWALGVNAYYMFGTINNYRNISFSSTNYTSTTELNYIEANNFRFRYGMQYFTGFNKK
                     NDLNIGLIFEPKKNFNVKSSKINSGVLTDTTNYDTDFDLPMLLGGGIYYTYNKKLSLG
                     IDYTFQNWANTRFYGKTDSLVNRNKISIGAEYLPNSSGRKYSDHIRYRLGLNVNNPYY
                     KIDGKSLDNNFGITFGIGLPLPYSRTMLNTSFEYGKIGNYSSLREDYFKFTFNVCVNE
                     NWFFKRKL"
     gene            7500..8873
                     /locus_tag="PLZ54_03310"
     CDS             7500..8873
                     /locus_tag="PLZ54_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ65996.1"
                     /translation="MKTKILVLLLAAIFSTNTFAQKSKNVQTPATTENSSEQVTDSMC
                     LIYISLFNEAAKNKQYADAIGPWTLAYNECPGANRVIYTRGREILQWKLSQAKDLTSY
                     KEAFDELMAMYDKRIKYFGDDSRYPTPWILGVKALDYITYVKGDDLKKPAYEWLEQSI
                     NGLKENSELEVLRQFIMLSYNLYKANPEHAEKFIADYLKVNDILETQIANPEYKSNEL
                     ANQIKQGLDVLFAQSGAADCQMLDKMYASKVTQNSTNLEFLNNVLSFYNRIGCIQSDV
                     YFSAALSAHKIQPSTESANALAEMSYKKKDYSGAIKFYEQATQLANNNKEKATYQYKI
                     AQIYYGDLKNYSRAREYALKSLEFNPNNGSAYLMIGIMYANSNIYDDPVLQKSVYWAA
                     VDKFIKAKQVDNDPKIIEEANKLIQTYINYFPSKEDIFFHPQLEEGKSFFVGGWIGES
                     TICRASN"
     gene            8962..9561
                     /gene="lptC"
                     /locus_tag="PLZ54_03315"
     CDS             8962..9561
                     /gene="lptC"
                     /locus_tag="PLZ54_03315"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOJ65997.1"
                     /translation="MFIVVMLFLSFACNRPTKPEIVQIESDPSLIPTLHATDITTVIS
                     DSGITRYRISAPQWDIYDKVPDPYWEFPYGIHLERFDMNLQVDANIHSKYARFFVNKK
                     LWELKNDVKATNLKGELFETEQLFWDQNSQKIYSDSLIKVTQATQIINAIGFESDQSM
                     NYYVFRNVKGIFPVDESKDTVPTSQSSNAGTPIRAMNAK"
     gene            complement(9593..10987)
                     /gene="glmM"
                     /locus_tag="PLZ54_03320"
     CDS             complement(9593..10987)
                     /gene="glmM"
                     /locus_tag="PLZ54_03320"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOJ65998.1"
                     /translation="MALIKSISGIRGTIGGRAGEGLTPLDIVRFSAAYAKFMKQNAGN
                     DSNKIVVGRDARISGDMVYHLVAGTLSGQGLEVVNIGLATTPTTELAVIYEKALGGII
                     LTASHNPMQWNALKLLNEKGEFLNAKEGEEVLKIAAEENFDFASVEQLGKISENFTFL
                     QKHIDNILSLNLVEVDAIKKADFTVAIDAVNSVGGIAVPALLEALGVKKVIKLNCEPT
                     GIFAHNPEPLPEHLSEISSLVKESKADVGFVVDPDVDRLAIICEDGSMFGEEYTLVAV
                     ADYVLQHTPGNTVSNLSSSRALRDITQKRGGKYSAAAVGEVNVVTLMKQTNAVIGGEG
                     NGGVIYPASHYGRDALVGIALFLSYLAKSKKTVSALKNEYPQYFISKNKIELTPSIDV
                     DKILATIKNNYQLQYEVNDIDGVKIDFPTGWVHLRKSNTEPIIRIYAEAKTKEQAEAF
                     ANAIMEEIRRIAAA"
     gene            complement(11018..11566)
                     /locus_tag="PLZ54_03325"
     CDS             complement(11018..11566)
                     /locus_tag="PLZ54_03325"
                     /inference="COORDINATES: protein motif:HMM:NF027435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4827 family protein"
                     /protein_id="HOJ65999.1"
                     /translation="MKKFIFLFLGFLPVILIFSSCNNTKTYAELLKDEKAIIASYIKR
                     SNIQVVTTFPADGEWDNKYVKTSSGLYFHLVSPGDTAANADTVAKNDRVVIRYRQYTL
                     TEYPDTIYYWNTIDYPYPISFTYLDYTQACTAWHEAVSYMKRNNSEAKLIVPSKIGLS
                     EYINSVTPLGYDFKIVGIQKSY"
     gene            complement(12355..15198)
                     /locus_tag="PLZ54_03330"
     CDS             complement(12355..15198)
                     /locus_tag="PLZ54_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOJ66000.1"
                     /translation="MDERDDREEITGAENEEDFVQQADIEDNSETKPSGHSSYKIPKI
                     ENSEVKHHLSGMYQNWFLDYASYVILERAVPDIYDGLKPVQRRILHSMRRLEDGRYNK
                     VANIVGHTMQFHPHGDASIGDALIQLGQKDLLIDCQGNWGNILTGDSAAAPRYIEARL
                     SKFALETVFNPKTTEWKLSYDGRNKEPVNLPVKFPLLLAQGVEGIAVGLNSKILPHNF
                     NELIDACIAYLKGEDFELYPDFQTGGLIDVSHYNDGERGGVVKIRAKINKVDNKTLSI
                     TEIPYGTNTSKLIDSILKANDKGKIKIRKVDDNTASSVEILVHLQPGTSPDKTIDALY
                     AFTDCEISISPNCCVIHDRKPRFMNVSEMLRVSCDHTKDLLEAELNIQKQELLEQLFF
                     TSLEKIFIENRIYKDKGFEDSSSQDAVVNYIDQRLEPFKENFIRAVTREDILRLLDIK
                     MVRITKFDSDKANDTLIAIQKKIDEVDFHLNHLIDYTISWYQGLKDRYGKNYPRRTEI
                     RNFETIIATKVAEANEKLYVNREEGFIGTSLKKDEFVCNCSDIDDIIVFFKDGRYKVT
                     KVAEKMFVGKNILYVNVFKKNDKRTIYNVVYRDGKAGPYYIKRFAVNGITRDKEYDLT
                     QGSAGSKILYFSANPNAEAEVIRVTLKPKPRMTKLVFEKDFGEIAIKGRQAMGNILTK
                     NEIHKITLKQKGGSTLGGRQVWFDHDVLRLNYDKHGEYLGEFQSDDLILIVSKNGEYY
                     TTNFDAANHYENNILIIEKFDENKVWSAVVYDGELKNYYLKRFQLTPSQKKNSFVGEH
                     PESRLILLSSVDFPRFEVVFGGHDARRNPLIVDAKEFISVKSYKAKGKRLTTFEVETV
                     NELEPVRFKEKNNEESTVINSDEEEDNENASENDRISNSSDAQESPEKATDVERKTNV
                     NSQTKEDAVSNAINEDRPEIHSTDEEGSQLTLF"
     gene            complement(15223..15972)
                     /locus_tag="PLZ54_03335"
     CDS             complement(15223..15972)
                     /locus_tag="PLZ54_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOJ66001.1"
                     /translation="MTSWTQRTELLIGKSGMEALQQASVLVVGLGGVGGMAAEMICRA
                     AIGKMTIVDHDTINPTNLNRQILASVNTISQYKTDVMAQRLLQINPDLKLKTFNQWAE
                     VSNIEELLETEKYDFVVDAIDTVTPKVFLIKSCLERGIDIISSMGSGGRMDPSKVRIT
                     DISNTHTCPLAKAVRQRLSKMGITKGLAVVFSDELPNKNAVLPVEDEKFKKSTVGTIS
                     YMPAIFGCMMASHVIREILRKNHFQKTLDRT"
     gene            complement(16272..17072)
                     /locus_tag="PLZ54_03340"
     CDS             complement(16272..17072)
                     /locus_tag="PLZ54_03340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ66002.1"
                     /translation="MLNASIVLYHHSIDTIKPLVETLRASNLISDIFLIDNSQSPNAD
                     FEKLPVTYIFTGKNLGYGAGHNIAMRETLKKNVPYHLVVNPDVAFEPEILKKIVDFMD
                     KNTNIGLLMPKVFYPDGEIQYLCKLLPTPFDLIFRRFLPERWTKKRTEKFEMRASGYD
                     KVIDVPYLSGCFMFFRTEALREVGLFDERFFLYPEDIDLTRRIRRKFRSVFYPHVSVV
                     HYHERGSYKNLRMLLIHIINMIKYFNKWGWFCDAERKRVNRDTMKQYF"
     gene            complement(17093..17797)
                     /locus_tag="PLZ54_03345"
     CDS             complement(17093..17797)
                     /locus_tag="PLZ54_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ66003.1"
                     /translation="MISVCMATFNGEKYITEQIDSILSQLGKEDELIISDDGSTDKTL
                     DFINELNDSRIKVFKNEGKHGYTHNFENALKHSSGDIIFFSDQDDVWLPNKVETILSY
                     LKKDCYVISDAYITNEKLEIQGKLSSWRSYKKGYFNNLYKSIYAGCTSAFTKQIKEYC
                     LPFPKTSLIQHDTWIGLLCELKFNVIYIDEPLIYYRRHDNNASGAGSKSTKSFFYMIQ
                     YRSVLLFETLKKVLFS"
     gene            complement(17832..18668)
                     /locus_tag="PLZ54_03350"
     CDS             complement(17832..18668)
                     /locus_tag="PLZ54_03350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ66004.1"
                     /translation="MENALITVLMTTFNEPVEYIQQSIESVLNQTYANFELLILDDSS
                     NEDAIQAIDSFKRDNRVSVVRDKQRMGFVNALNKGMKMAKGEYIARMDADDICFNDRF
                     EKQINFMQTHSDVDILGGNMKIIDENGIVVSQRQYPCSKFSLKFSSVFRSPVAHPTVM
                     FRRSIIEKNMFYDDLFKKAEDLEFWLRLRNNGFKIANMPEYVLYFRISGDLAKKRNSE
                     HFSYSFRARSKNFSWRYFYLDIPSILFAKLYSIVPSKITSIYYLNENKNMKNGVPKSK
                     KQ"
     gene            complement(18671..19237)
                     /locus_tag="PLZ54_03355"
     CDS             complement(18671..19237)
                     /locus_tag="PLZ54_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOJ66005.1"
                     /translation="MNYTKDISEYFNRVKQTIERVSIEDINLLMNILLKAKDEGQNVF
                     VMGNGGSAATASHMCCDFNKGISYGKEKMFRFICLNDNIPTMMAYANDLGYEEIFVGP
                     LKNFLQEGDLVIGISGSGNSRNVVKALEYANQAGAITIGLTGYSGGKVKQICKYNVHI
                     PIDDMQITEDLHMILDHCIMSILSQKED"
     assembly_gap    19408..19507
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19508..20382)
                     /locus_tag="PLZ54_03360"
     CDS             complement(<19508..20382)
                     /locus_tag="PLZ54_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HOJ66006.1"
                     /translation="MIISRTPFRISFAGGGSDLPSFYREETGAVVSTTIDKYMYLAIH
                     PYFDKSKIQLKYSKTELVDSIEEIRHPIFREVLSLYNLKGIDLNSIADIPAGTGLGSS
                     SSFTVGLLNVVNAYLNKACSSQKLAELACDIEINKVGSPIGKQDQYAAAFGGLNFITF
                     YGDETVNVEKIIMNPEKKKELQDNLMMIYIGGKHSAKDILKEQQAIKNGSKKFSSQRE
                     MVKQAYQMKSLLENNNIDDVGRILHEGWMLKKTLTDRISNDHIDEIYNRGIKAGALGG
                     KLLGAGGAGFILFYCP"
     gene            complement(20385..>20670)
                     /locus_tag="PLZ54_03365"
     CDS             complement(20385..>20670)
                     /locus_tag="PLZ54_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOJ66007.1"
                     /translation="KQAYLNAIYFCPHHPDAGYPEERKEYKIQCNCRKPAPGMLFHAA
                     KDWNIDLTNSIMIGDRETDVKAGINAGVKMSLMIERNKPYELLNTLKQIL"
BASE COUNT         6462 a         3991 c         3445 g         6672 t
ORIGIN      
        1 ttttctttac cttttatttc ccctgaaaaa gttgcattaa ttacttgaat ttacgcttta
       61 taatagatat gaaccatgaa ttaattcaat tatgggacaa aatagactga agttactttc
      121 ttactttgag aaggaattgg tatctatatt ttgccaatgg ccccagagtg tttcaaatgg
      181 agaagggtag ggtggatgtt gctcaaacat ttgtacaatt tggagataaa cgataactga
      241 attattgaat acggaatgtt taatttcagc atttttgcag aagtgtgttt tttgaatacc
      301 gtttgccgtt tgatcagaac gattttgtcc attttcggca aaccaatcgg cgaagacggc
      361 atacaaatta acgctttatt ggctactcaa gcttggaatt tgaaaaaaat gatgtgaaaa
      421 ctcaaagaag agttttttgt ttcatttcaa caaaaatttt ataatcttac cactaaaaca
      481 aacttttaag gattgactaa tcatacttga caaatcttta aatttactcc atcctaactt
      541 tctcatccaa cagagcgaaa ttgatgactt ccattatatc gtttacgtag tgaaaatgaa
      601 gacctttcaa ataagacgca ttaatttctt ccacatcttt tttgttctct tcgcaaagaa
      661 ccaattcggt tatgcctgct cttttggccg caagtatttt ttctttaatt ccaccaaccg
      721 gtaatacttt tccacgtaag gtgatttcgc cggtcatggc aagatttttc cgaacttttc
      781 ttttggtaaa tgccgaaaca agcgctgtaa gcattgtaat tcccgccgac ggaccatctt
      841 tgggaatagc tccttcggga acatgaatgt gaacattcca tttttcaaac atttcactat
      901 caattcctaa ctgttcggcg tgtgctttta tgtattccaa tgcaatcaca gcagattctt
      961 tcatcacttc gcccaaattt cccgttaaag tcaactgagg agatttactt ttgcttagac
     1021 tcgactcaat atagagaatt tcgcccccca cttgtgtcca tgctaaaccg gtaacaaccc
     1081 cggcataatc gtttccctga tatttttctt ttgtatatcg cggaactccc aaaaaagttt
     1141 tcaaatcatc tacagtaagt tgcacattat aaggctcatt ggaagccact ttttttgcaa
     1201 ttttccgcat gagcgaagca atttgtctgt ccaattcgcg aacgcccgat tcacgcgtat
     1261 aattttcgat caaatgttcg attacaggct tcgtcaaaat caactgttct tttgttaaac
     1321 cgtgattttc gagctctttg ggaacaagat ggcgtcgggc aatttccact ttttcttcct
     1381 gtgtatagcc acttacttct atcaattcca tcctgtcgag taaaggacga ggaatggtat
     1441 tcagattgtt ggctgtagca ataaacatca ctttcgacaa atcgaaatcg acgtcgagat
     1501 aattatcgtg aaaactggta ttctgctcgg ggtcgagaac ttcgagcaaa gcagaagacg
     1561 gatcaccttt ataatcggca gcaaccttgt caatttcatc caacacaaaa accggattgg
     1621 ccgactgtgc tttctgaata ttctgcataa ttcttcccgg caacgctccg atataagttc
     1681 ttcgatgacc gcgaatttcg gcttcgtcat gcaaaccacc aagcgaaatt cttacatatt
     1741 ttctgttcaa cgctctggct atggatttgc ccaacgaagt ctttccggtt cccggcgggc
     1801 cataaagaca aataatgggc gatttcatgt ctcccttcaa tttcagcacg gccaaatgtt
     1861 ccaaaatacg ttcttttacg gtttccatgc cgaaatgatc ctgttcaagg attttacgtg
     1921 catttttcaa attaaaatta tcttttgtgt actcattcca tggcaattcc aataaagttt
     1981 ccacataatt cagctgcgta gtataatccg gagaatgagg cgacatacgt tccagttttt
     2041 tcaattcctt ctcaaacaaa gcagccacat tttcattcca ttttttttgt tttgcttttt
     2101 ctctgaaatc cagaatatcc tgttcgggag aagctccacc caattcttcc tgaatggttt
     2161 tcatttgctg ctgcaggaaa tactgcttct gctgctgatc gataccttct ttggctttat
     2221 tttcaataga aatttttatt tccagcaatt gccattcctt gttcaacaaa tcgagcaatt
     2281 gatagccccg atccagaata tcgtcaattt caagcagtct ttgcttttcc tgtacattca
     2341 atccaaaatt cacacataca taattaataa gaaaaacggg gttatcaata ttacgtatgg
     2401 caaaaactgc ttctggaggc attattcctg cattggctat aatttttgca gccatatcct
     2461 taatggtagc aaccagtgcc ggaaaaacac catcttcagc gcttggaatt atttcgggag
     2521 ccaaattaat ccgcgctttc atataaggct tcagtgtcaa aacttcaccc agaaaaaaac
     2581 gtgattttcc ttcaagaata accgtagtag tgttatcgtt catttccaaa atccgtacaa
     2641 ctttagccac agttcccata gaatacaact ggtcaggagt cggatcatca atatttttat
     2701 ctttttgtgt gcaaacgcct atataatgat tgctttcttt tgcttcccgc acaagtttca
     2761 gcgacttcgg tcgagcaacc gataccggta ataaaacacc tggatacaaa accatgtttc
     2821 tcaagggtag aataggcaat atatcgcccg actcgagaga aacatctcca tttttttcct
     2881 cttcaaacga aataatggga aaagcttccg aaggttgtgt taattcttca atattaaaat
     2941 catcaaatga atgtttcatt gcgctattta aaattttaat gacaaaatta gtatctttaa
     3001 tgtcagaatg acataaattt attcaaattc aacaaaatgg gtttccttct tttctaaaaa
     3061 atcttcagaa aatgggtcga cgatattttt ttcaattgtt gtgccaaaaa atctgtatta
     3121 aaaaccggca gttctaaaaa tggtttttca caaaataccc atttaaatct ttcatcagaa
     3181 tataatttat ttttttaaag gtctgatggg acacacatgt ctgaatatta tattcattcg
     3241 tgtgtatgca ctacatgtgg gttttcttat tataaaattt tttttcgtgt gggagaaaaa
     3301 gaaaacaaat gattgaaaaa atacgaaaag gaaattaaat atatagttca ggaaatcaaa
     3361 taattaaata tattaagagg ttaaaaaatt acttattgtt gggttttaaa gttttgacca
     3421 aaagccttag ctgtaatcga ttccaccttt actttccata ctttcacgtt tctaagtgcc
     3481 ggaagcgagt atttaaaatc gctgcgttgt gtatattttt tcataaacaa attcatggcc
     3541 tgttcttttt cttccaaatt ttcgataaaa atcaccttcc ctttcaccag cacacttttg
     3601 gagttcatgc tgtaactgca tgccacatca ggatgttggt acattaattt cctgtccgaa
     3661 caaaacgtaa tacagacacg attatccttt tggagtaata gctgatgttt tccttcaggt
     3721 gcagaatgta aaataacttc gttatgatca aaagcaaaat tcatgggtat aacataagga
     3781 cttccatcca attccgaaat acctacaaag caaatatcac aggcattgat aatattttca
     3841 atttcaactt ggtcttcaat aaaaaaagtt ctcatataaa ttctgtttaa aagacaaata
     3901 taatcaaatt tctttgtgat ttatcatcga aatgaaacta ttataaaatc ttggcataaa
     3961 atttgttaat aaacaatagc ttcacttaag cgaaatccta gaaaacgaga tagaatagaa
     4021 aacgttttcg taaaaaaatt tttttttcac actgcaacta aaagtaatta ttcaaaagtc
     4081 caacaaaact caggcaaaaa caagaaacta ataattttac tttattgaat acgaagtatt
     4141 taattgtttt tgagagggca caaaatattt agaaaaataa aaatgaaaaa aattgaaaaa
     4201 aatatcgcaa atacttgcat ttataaagaa aaaaagatgt acctttgcat cgattttgaa
     4261 ttgtaaacaa acatgtaaaa cttcaaaaaa ttaagaaaat gaaaaaatta tttttattgt
     4321 gtgctgttgt atttgcattc gttgcatgtg gaccaaaaga aaacaaagaa gctgctgcta
     4381 ctgaagattc tgtagttgtt gttgatactg ttgttgctcc ggttgatact gcagcagttg
     4441 acacaactgt ggctcctatg taattttaaa acaaccaaaa tttacagagc tgaagtccgt
     4501 tagtgttagc ggactttttt cgtttataca tgtagcgaat tataaaaaac tcaaatccca
     4561 cttttacttc tttacctgaa taagaaatta tatgtaaatt tgtaaaccga ttgtcataat
     4621 aaagaaaaaa attgtttatg ctaagaattg cagttcaaac caaaggaaga ctttttgatg
     4681 acagcatgtc gttacttaac gaagccggta ttaaaataga attgggaaaa cgtcttttgc
     4741 ttattccggc acaaaatttt cctttagaag ttttgttttt acgtgatgac gatattcccg
     4801 aatctgttgc cgatggcgta gccgatattg gtattgttgg agaaaacgaa tttgctgaaa
     4861 agcaaaaaaa tgtcaaactt attcgtcgat tgggattcag taaatgccgc ctctcgctgg
     4921 cagttcccaa agaagcagaa tattctgata tctgctggtt tgaaggcaaa accatagcaa
     4981 cttcttatcc cgtaattttg agtaattttc tgaaagaaaa agggatacgt gctgagattc
     5041 atgttataac aggttcagta gaaattgctc ccagcatagg tcttgcagat gccattttcg
     5101 acattgtcag ctcaggaagt acactggtaa ccaatcatct gaaagaagta gaggttgtta
     5161 tgaagtcaga agccttgctg attgcgaatc caaaactctc tgaagcgcaa aaaaagatac
     5221 tggacgagtt acttttccgc atagaagccg taaaaatggc tgaagacaaa aaatacgttt
     5281 tgatgaatgt tccaacctct cagctggaaa aaattatcga aataattccc ggtatgaaaa
     5341 gtcctaccgt catgccattg gctaaagaag gatggaactc tgttcacgca gtaattgccg
     5401 aaaaagaatt ttgggatatt attggaaaac tgaaagcttg cggtgctgaa ggaattctgg
     5461 tattacccat tgaaaaaatg atcctgtaaa tttctatcga aatatgaatc tttgcatcga
     5521 tcagggaaac tcaagtatca aaatgggaat tttcgaccaa agcaaactgg taaaaacttt
     5581 tgtatataaa aattttgatc gaaaatctgt tttatcaatg ctccgtaatt atcctgtcga
     5641 tcggtgtatt tactccagcg taatcaataa aaatgcttca gccgttgcat ttttaaagaa
     5701 aaatattgcg caagtattac tgctgacaca tgaaactccc ttgcctatcg aaaacaaata
     5761 ccaaacgccc aaaactctgg ggaaagaccg gttggcagct gttgtcggtg caggttttct
     5821 cgagcccaat cgtgatgttt tggtaatcga tgcaggtact gccataactt tcgatttcat
     5881 ggatgccaag agaaactatt ggggtgggaa cattgatgtc ggcttggaat tgagattaat
     5941 ggcgctgcat acatttgcaa aaaaattgcc tttggtgaaa ccggttcagg aagttccctt
     6001 aatggcctcc aatactgaaa tggcattggc tgcaggcgct ctgaacggaa taatatttga
     6061 aatcgacggt tatattaatg ctttgaaaat cgaatatcct gacctttcaa catttttaac
     6121 aggcggaagc ttgatttttt ttgataaaaa attaaaaaat agcatctttg tagaaaaaaa
     6181 tttggtgtta atcggactca attatatact tcaatataat gtacaataaa aagattttaa
     6241 ttgcattttt aatattgttt ttatctatgc aggatacttc cagtcaaaac aatacaaact
     6301 caccctatac gcgttttggt tttggtgaaa tcaacgacaa ccacaacagc gaacaaagag
     6361 caatgggagg tgtggctttg ggggttcgtt ctgtttcgag tatcaattct atcaatcctg
     6421 catcctacag tgtggttgac agtatgactt tcatgttcga tttcggagtt tcggcgttac
     6481 gctcgcaatt taaagatcca agtggaaaaa gttcaaactt tactgccaat ctcgattata
     6541 tcaccattca atttcctctt tcaaaaaaca tagctttaag tgccggcatt cagccttatt
     6601 cgttttcggg ctacaatttc tatactacag aaatacttac cataccaaat aatagtaata
     6661 ctccggacag ccttaatgta acgaaaagtt ttaccggatc aggtggattc agtgaagttt
     6721 atatgggatt gtctatcgat tttttgcaac attgggcatt gggggtcaat gcttattaca
     6781 tgtttgggac aattaacaat tatagaaaca taagcttttc ttccactaat tacacctcta
     6841 ctacagaatt gaattacata gaagccaata atttccgttt tcgttatggg atgcagtatt
     6901 ttactggttt caacaaaaaa aacgatctaa atatcggttt aatattcgaa cctaaaaaga
     6961 attttaacgt caagtcttcc aaaattaatt cgggagtatt gaccgacaca accaattatg
     7021 acacagattt tgatcttccc atgctactgg gagggggaat ttattatacc tacaacaaaa
     7081 agctttcact cggcatcgac tatactttcc aaaattgggc caatacaaga ttctacggca
     7141 aaacagacag tttggtcaat cgaaacaaaa tttccattgg agcggaatat ttgcccaact
     7201 cttccgggag aaagtacagc gaccatatac gttatcgttt aggattaaat gtaaacaacc
     7261 cttattataa aattgacgga aaatcgcttg ataataattt tggtataact tttggtattg
     7321 gattaccgtt accctacagc cgaacaatgc taaacacttc ctttgaatat ggaaaaattg
     7381 gtaattacag ttctttgagg gaagactatt ttaaatttac atttaatgtt tgcgtcaatg
     7441 agaattggtt ctttaaaaga aaattataaa acaaacaaaa ttttaaaagt cataaaaaaa
     7501 tgaaaacaaa aatattagtg ttgttgttgg ctgcaatatt ctcaaccaac acgtttgccc
     7561 aaaaaagcaa aaatgttcaa acaccggcta ctacagaaaa cagttcagaa caggtaaccg
     7621 attccatgtg tttgatttac attagtcttt ttaacgaagc tgcaaaaaac aaacagtatg
     7681 cagatgccat aggcccttgg actttggctt ataatgaatg ccccggagca aacagggtaa
     7741 tttatacacg aggaagagaa attttgcaat ggaaactttc acaagccaaa gacttaactt
     7801 cttacaagga agcttttgat gaattgatgg ccatgtatga caaaagaata aagtattttg
     7861 gtgatgacag ccgatatcca actccttgga tacttggggt aaaggctttg gattatatta
     7921 cttacgtaaa aggtgatgac ttgaaaaaac cggcttacga atggttggaa caatccatca
     7981 acggactcaa ggaaaattct gaattggaag tacttcggca atttataatg ctttcctata
     8041 atctttataa agcaaatcct gaacatgccg aaaaatttat tgccgattat ttaaaagtta
     8101 acgatatact tgaaactcaa attgctaatc cagaatataa atccaacgag ctggccaatc
     8161 aaatcaagca ggggctggat gtgttgtttg cacaaagcgg tgcagcagat tgtcaaatgc
     8221 tggacaagat gtatgcctca aaagtaacac aaaattctac caatctagaa tttttaaata
     8281 acgttttaag tttctacaat cgcataggat gcatacaatc agatgtttat ttcagtgctg
     8341 cattgtctgc tcataaaatt caaccttcaa ctgaatcggc aaatgcattg gcagaaatga
     8401 gttataaaaa gaaagattat tccggtgcca ttaaatttta tgaacaagcc actcaactag
     8461 caaacaacaa caaagagaaa gctacctatc agtataaaat tgctcaaata tactatggcg
     8521 acctcaaaaa ctattctcga gccagagaat atgcactgaa atcgttagaa ttcaatccca
     8581 ataacggaag tgcctatttg atgataggca tcatgtacgc caattccaat atctatgatg
     8641 atccagtatt acaaaaatcg gtgtattggg cggctgtcga taaattcata aaagcaaaac
     8701 aagttgataa tgaccctaaa attattgaag aagccaataa attgattcaa acttacatta
     8761 attatttccc atcaaaagaa gatattttct tccatcctca actggaagag ggtaaatctt
     8821 tttttgtagg cgggtggatt ggcgaatcaa ccatttgcag ggcaagcaat taaaatcatt
     8881 cgaataaata aaataaaggc atcccttagg gatcttatga aacagatatt aaaaaattat
     8941 ttttcaaaaa gcataacaac tatgtttata gttgttatgc tttttttatc ttttgcatgc
     9001 aatcgaccaa ctaaacctga aattgttcaa attgaatctg atccttcttt gattccgact
     9061 cttcatgcta cggatattac taccgttatt tccgattcag gaattacccg ctaccgtatt
     9121 tctgctccgc aatgggatat ttacgataaa gttccggatc cctattggga atttccttat
     9181 ggcattcact tggaacgttt cgatatgaat ctgcaagtag atgccaatat tcacagcaag
     9241 tatgcccgtt tttttgttaa taaaaaattg tgggaactga aaaatgatgt aaaagccacc
     9301 aatctgaaag gtgaactttt tgaaaccgaa caactttttt gggatcagaa cagtcaaaaa
     9361 atttattccg acagtttgat taaagttact caggcaaccc aaatcattaa tgccattgga
     9421 tttgaatcgg atcaatccat gaattactat gttttccgca atgttaaggg aattttccct
     9481 gtggatgaaa gcaaagatac tgttccgact tcccagagca gtaatgccgg cacaccgatt
     9541 agagcaatga acgccaagta aaaattttcc aaaaatttgt tattcggaaa attcaagcag
     9601 cagctatccg ccgaatttct tccataatgg cgtttgcaaa agcttcagcc tgttctttgg
     9661 ttttggcttc ggcgtaaatt ctgataatcg gctcagtatt cgatttgcgt aaatgtaccc
     9721 aaccggtagg aaaatcgatt tttacaccat caatatcatt aacttcgtac tgaagttgat
     9781 aattgttttt aatggttgca agaattttat cgacatcaat agacggcgtc aattcaattt
     9841 tatttttaga aataaaatac tgtggatact catttttcaa tgcagacaca gtttttttcg
     9901 actttgccag ataagaaaga aaaagtgcga tacctaccaa cgcatcgcgg ccataatgac
     9961 ttgcaggata aataactccg ccattacctt caccaccaat tacagcattc gtttgtttca
    10021 ttaaggtaac cacattcact tcaccaacag cagcagctga atattttcca ccccgttttt
    10081 gtgtaatatc gcgtaaagct ctcgacgaac taagattgga aacggtattt cccggtgtat
    10141 gctgcaatac ataatctgcc acagccacca acgtatattc ttcaccaaac atgctgccgt
    10201 cttcacaaat aatggctaaa cgatccacat caggatcaac cacaaaacct acatcggctt
    10261 tagattcttt taccaacgac gagatttcag acaaatgttc gggaagcggt tcgggattgt
    10321 gagcaaaaat tcctgtcggt tcgcaattga gtttaataac ttttttaacg cccaacgctt
    10381 cgagcaaagc cggtactgca attcctccca ccgaatttac tgcatctatg gcaaccgtaa
    10441 aatcagcttt ttttatagca tcaacttcta ctaaattcag cgacagaata ttatcaatgt
    10501 gtttctgcaa aaaagtaaaa ttttcggaaa tttttcccaa ctgctcaaca gaagcaaaat
    10561 cgaaattttc ctcggccgct attttcaata cttcttcacc ttccttcgca ttcaggaact
    10621 cacctttttc gttaagcagt ttcaaagcat tccactgcat tgggttgtgg ctggcagtaa
    10681 gaataatgcc acccaaagct ttctcgtaaa tgacagccaa ctctgtagta ggcgtagtgg
    10741 ccaatccaat attaaccacc tccagtcctt gacccgataa ggtaccggct accaaatgat
    10801 ataccatatc tcccgaaatt cgggcatcac gtcccactac aattttattt gaatcattac
    10861 ctgcattttg tttcataaat ttggcataag cagccgaaaa ccgaacgata tccagaggtg
    10921 ttaatccctc tcctgctcgg ccaccaatag ttccacgaat tccggaaata gattttatca
    10981 gtgccatata aaacttaatt agaagtttgt taaaaaatta atagctcttt tgaatgccaa
    11041 caatcttaaa atcgtatccc agtggagtta cgctgtttat atattcgctc aagcctattt
    11101 ttgaaggaac aataagtttt gcttcagaat tgttgcgttt catataggaa acggcttcgt
    11161 gccaagccgt acaggcttgc gtataatcaa gataagtgaa agaaatcgga taaggatagt
    11221 caatggtatt ccagtaataa atggtatcgg gatattcggt cagtgtatat tgtcgataac
    11281 gaataacaac ccgatcattt tttgctaccg tgtcggcgtt ggcagcagta tcgcccggac
    11341 tgaccaaatg aaaatataga ccacttgaag ttttcacata tttattatcc cattctccat
    11401 cagcaggaaa agttgtaact acctgtatat tactacgttt aatataactt gctataatgg
    11461 ctttttcatc ctttaaaagc tcggcgtaag ttttagtgtt gttgcacgaa gaaaaaatta
    11521 ggataacagg caaaaaaccc agaaacaaaa aaatgaattt tttcataaaa ccgtataaat
    11581 taattttata caaatgtacc ataaaaaagt agaaagaaaa agttaagaaa ctcaaatcta
    11641 tggattgaaa atccataggt tggattgcaa ataaaattcg ctttaatttt ggctataagt
    11701 caattaaaat gttaaattaa tgttcataaa cttatttaaa aatgtaattt tcattagaat
    11761 tggattgttt tttgtgatcc aaataggaat ttatcatctc atccgttacc ttgtctgtac
    11821 tcgaacatcc acaaccttgc gtataaaaat gattaatcca accccgacta tgccgttcct
    11881 gaaatttcct gcaacaacat ccctgaactt ttccctttta atcgcttttt atatcgctta
    11941 aacttatttg aaatggctgg cttaaaaaaa catcttcctt actcaccact tctttgaaaa
    12001 tctctacttg ttcccgttgc atatttagcg taaaatatct caacaacgca gtggttttcc
    12061 gttgaaatac tgcataaaca tctttgtaat cacactttat gaatatgaac ttgtaaactg
    12121 tatatgctat taccgctttc ctgtttattc atctcatttt tatggcaaat ttaatagcag
    12181 ttttcatcag aatataattt attttttaaa ggtctgatgg aacccacatg tctgaatatt
    12241 atattcattt gtgtttgtgt gtgcaataca tgtgggtttc ttaaaactaa cgtttttgaa
    12301 aaaatttgaa ataaggaagc ataagttatt gcctatggtt gactgtatat taaatcaaaa
    12361 caaggtcaac tgactgccct cttcgtcagt tgagtgaatc tccggcctgt cttcgtttat
    12421 agcatttgaa acggcatctt cttttgtttg ggaatttacg ttggttttcc tttcaacatc
    12481 agttgccttt tccggcgact cttgtgcatc agaagaattt gatattcgat cgttttccga
    12541 agcgttttcg ttatcttctt cctcatcgct gttgattacc gtactttctt cattattctt
    12601 ttctttaaac cgaacaggct ccaattcgtt aacagtttct acttcaaaag ttgtcaaacg
    12661 ttttcctttg gctttgtaac tttttacact gataaattct ttagcatcaa ctattaaggg
    12721 atttcttcgg gcatcgtgtc caccaaaaac aacctcaaat cttggaaagt cgacactcga
    12781 aagcagaatc aatcgagatt ctggatgttc accaacaaag ctgttttttt tctgcgatgg
    12841 agtaagttga aatcttttca gataataatt ttttaattct ccgtcatata ccactgccga
    12901 ccaaacctta ttttcgtcaa atttttcaat aatcagaata ttgttttcgt aatgattggc
    12961 agcatcaaaa ttcgtggtgt agtattcgcc gttttttgaa acaataagaa tcaggtcatc
    13021 gctttgaaac tctcccaaat attctccatg tttgtcgtaa ttcaatcgta aaacatcatg
    13081 gtcgaaccaa acctgacgtc ctccgagcgt agaacctccc ttctgtttga gcgtaatttt
    13141 atgaatttcg tttttggtca gaatgtttcc cattgcctgg cggcctttta tggcaatttc
    13201 tccaaaatct ttttcaaata ccagcttggt catgcgcggt ttgggtttca acgttacacg
    13261 aattacttcg gcttcggcat tgggattggc tgaaaaatac aaaatttttg aaccggcaga
    13321 accctgcgtc aaatcgtatt ccttgtcgcg agtaatgccg ttgacggcaa aacgtttgat
    13381 gtaatatggt ccggctttcc catctctgta caccacgtta taaatcgttc gtttgtcatt
    13441 ttttttgaaa acattaacat agagaatatt tttccctaca aacatttttt cggcaacttt
    13501 ggtaaccttg tatcttccat ccttgaaaaa cacaataata tcgtcaatat ctgaacaatt
    13561 gcaaacaaat tcatcctttt tcagcgaagt tccaataaaa ccttcttcac ggtttacata
    13621 tagtttttcg ttggcttcgg ccactttggt tgcgataatg gtttcaaaat tacgaatctc
    13681 cgtgcgccgg ggataatttt tcccgtaacg atcctttaaa ccctgatacc aagagatggt
    13741 ataatcgatt aaatgattta agtgaaaatc cacttcatca atttttttct gaatagctat
    13801 cagcgtatcg ttagctttgt cggaatcaaa tttggtaatg cgaaccattt taatgtcgag
    13861 tagtctcaat atatcttccc ttgttacggc tctgataaaa ttttccttga agggttccaa
    13921 acgttggtcg atataattga ctacggcatc ctgacttgag ctgtcttcaa aacctttatc
    13981 cttgtaaatt ctgttttcaa taaaaatttt ttccagtgat gtaaaaaaaa gctgctccaa
    14041 aagttcctgc ttctgaatat taagttcggc ttccaataaa tctttggtat gatcgcagct
    14101 gacgcgaagc atttcgctga cattcataaa ccgcggtttc cggtcatgaa tcacgcagca
    14161 gttgggcgat atgctgattt cacaatcggt aaaggcatac agcgcatcaa tggttttgtc
    14221 gggtgaagtt ccgggttgca aatgaaccaa tatttcgacg ctggacgcag tattgtcgtc
    14281 aactttcctg atttttattt ttcccttgtc gttggccttt aaaatggaat caatcagttt
    14341 cgacgtattt gtcccgtaag gtatttcggt gatagacaat gttttattgt caaccttatt
    14401 gattttagct ctgattttaa ccactcctcc gcgttcgccg tcattgtaat ggctgacgtc
    14461 gataagtccg cctgtttgaa aatcgggata cagttcaaaa tcttcacctt tcagataagc
    14521 aatgcaagca tcaatcaatt cgttaaaatt gtgaggcaaa attttagaat tcaatccaac
    14581 tgcaattcct tccactccct gagccagtag taatggaaat ttcaccggca aattcaccgg
    14641 ttccttgttc cgaccgtcgt aagatagttt ccactccgtt gttttagggt tgaaaacggt
    14701 ttcgagtgca aatttggaaa gacgtgcctc gatataacga ggtgcagctg cagagtctcc
    14761 cgtgaggatg tttccccagt ttccctgaca gtcgataagc aaatctttct gtcccagttg
    14821 aataagcgca tccccaatgg aagcatctcc atgcggatga aattgcatgg tatgccccac
    14881 aatgttggcc accttgttgt aacgaccatc ttcgagtctt ctcatagaat gaagaatacg
    14941 tcgctgcaca ggtttcagtc catcgtaaat atcgggtacg gcacgttcaa gaattacgta
    15001 ggaggcgtaa tccagaaacc agttctgata catgccggaa agatgatgtt ttacttcact
    15061 gttttctatt ttcggtattt tataagaaga atgcccggaa ggtttcgtct ctgaattgtc
    15121 ttcaatatcg gcctgctgaa cgaaatcttc ctcattttcc gctccggtta tttcttccct
    15181 gtcatctctt tcgtccatat attaatttac tccatttttt tcctaagttc tatctaaagt
    15241 tttctggaag tgattttttc ttaaaatttc acgaattaca tgagaagcca tcatacagcc
    15301 aaaaatggcc ggcatatacg aaatggttcc cacagtagat tttttaaact tttcatcttc
    15361 aacaggtaat acggcatttt tgttgggcaa ttcatccgaa aagactacgg caaggccttt
    15421 tgttatgccc attttcgaca gccgctgccg tacagctttt gccaatggac aagtatgcgt
    15481 attggaaata tccgtaattc tgacttttga aggatccatt ctgcctcccg aacccattga
    15541 ggaaataata tcgatacccc gctccaaaca gctctttatt agaaaaactt tgggcgtaac
    15601 cgtatcaatg gcatctacca caaaatcgta tttctctgtt tcaagcaatt cttcgatgtt
    15661 cgatacttct gcccattgat taaaagtttt taattttaaa tcagggttga tttgcaacaa
    15721 cctttgggcc atcacatcgg ttttgtattg cgaaatcgtg tttacagacg ccagtatctg
    15781 cctgtttaaa ttggtaggat tgatggtatc gtgatccaca attgtcattt tgcctatagc
    15841 ggcacggcaa atcatttctg cagccatgcc acccacaccg cctaaaccca ctacgagcac
    15901 cgatgcctgt tgcaaggctt ccatgcccga ctttccaatc agtaactctg tacgttgtgt
    15961 ccacgaagtc atatgggtat aattgtcata caaagttaaa cttttgcgag gacaaatcca
    16021 tagttttgga agagaaattt tttgatcggt ttgaataaaa tggagtagag ttgcaagttt
    16081 gagaccaata acctcaaatt gaaagaattt gggttagaag gttatagtag ttatatgtta
    16141 tttgttacat tcttttatgc atgtttacag agttgaattt tagtttttga atttagaaat
    16201 taagttttcc aaaaaatatc gatagatatc ataaattaat ttatactttt aattttaatc
    16261 tcatggcatt gctaaaaata ctgcttcata gtatcacgat ttactcgctt tcgttcggca
    16321 tcacaaaacc aaccccattt attgaaatat tttatcatat tgataatgtg aatcaacaac
    16381 atccgcaaat ttttataaga accgcgttcg tgataatgta ccaccgaaac atgaggataa
    16441 aaaaccgacc gaaattttcg gcgtattcta cgcgtcagat cgatatcttc aggatacaga
    16501 aaaaaacgtt cgtcaaacag gccaacttca cgaagcgctt cggtacgaaa aaacatgaaa
    16561 caacccgaaa gataaggaac atctattact ttatcgtaac cggaagctct catttcaaac
    16621 ttttcggttc gtttttttgt ccatctttca ggtaaaaaac gccgaaatat caagtcgaaa
    16681 ggagtaggaa gcaatttaca aagatactga atttctccat caggataaaa tacttttggc
    16741 attaaaagtc caatatttgt atttttatcc ataaaatcaa caattttttt gagaatttca
    16801 ggttcaaaag caacatcagg attgacaacc aaatgatacg gcacgttttt tttcaatgtt
    16861 tcacgcatag caatattgtg cccggctcca tagcccaaat ttttccctgt aaaaatatac
    16921 gtaacgggaa gtttctcaaa atcagcgttg ggagactgag aattgtcgat aaggaaaata
    16981 tcggaaatga gattagacgc gcgaagtgtc tcaaccaaag gttttatcgt gtcaatggaa
    17041 tgatgatata aaacaataga agcattcagc atgaaatagt ttttttgaaa aactatgaga
    17101 ataaaacttt tttcaaggtt tcaaataata atactgagcg gtattggatc atataaaaaa
    17161 atgatttcgt actttttgaa ccagcacctg atgcattatt atcatgtctt ctgtaataaa
    17221 taagaggttc atcaatataa attacattga atttcaattc acaaagcaaa ccgatccacg
    17281 tatcgtgttg aatcaatgaa gttttaggaa aaggaagaca atattcttta atttgttttg
    17341 taaaagcact ggtacaaccg gcataaatac ttttgtacaa attgttgaaa tacccttttt
    17401 tataacttct ccaagaactt aatttacctt gaatctccag tttttcattt gtaatataag
    17461 catccgaaat aacataacaa tcttttttca aataggatag aattgtttca actttattgg
    17521 gaagccaaac atcgtcctga tcggagaaaa aaataatatc tcctgatgaa tgttttaaag
    17581 cattttcaaa attatgtgtg taaccgtgct ttccttcgtt tttaaatact tttatacgac
    17641 tgtcatttaa ttcgtttatg aaatccaaag ttttatctgt tgaaccgtca tccgaaatga
    17701 taagctcatc ctccttcccc agttgactca gaatagaatc gatttgttct gtgatatatt
    17761 tttctccatt aaaggtagcc atgcatactg aaatcataga aataatttta gatgtagcag
    17821 ttaaaaattc ctcattgttt ttttgatttt ggtacaccgt ttttcatgtt cttattttca
    17881 tttaaataat agattgaagt aattttggag ggaacgatcg agtataattt tgcaaacaat
    17941 atagatggaa tatctaaata gaaataacgc catgaaaaat tttttgagcg tgcccggaaa
    18001 ctgtacgaga aatgttcgga atttcttttc ttggctaaat cacctgaaat tctgaaatac
    18061 aacacgtatt ccggcatatt tgcaatttta aatccattat ttcttaaccg taaccaaaat
    18121 tcaaggtctt cggctttctt gaataaatca tcgtaaaaca tgtttttttc gatgattgat
    18181 cttcgaaaca taactgtcgg gtgggctaca ggacttcgaa aaacagaaga aaatttcaaa
    18241 gagaatttag aacacggata ttgacgttgt gaaacgacga ttccattttc gtcaatgatt
    18301 ttcatgtttc cacccaaaat atctacatcc gaatgagttt gcatgaaatt gatctgtttc
    18361 tcgaatcggt cgttaaaaca aatgtcgtcg gcatccatac gggcgatata ttcacctttt
    18421 gccattttca ttcccttatt taaggcattt acgaaaccca ttctttgttt atctctaacc
    18481 acagacactc gattgtccct tttgaaagaa tcaatggctt gaattgcatc ttcatttgag
    18541 ctgtcatcta aaataagcaa ttcaaaattg gcataggttt gatttaatac actttcaatc
    18601 gattgttgaa tatattcaac cggctcgttg aaagtagtca tcagcacagt aattaaagca
    18661 ttttccattt tcaatcttct ttttggctta aaatactcat aatgcaatga tccaaaatca
    18721 tgtgcaaatc ttctgttatt tgcatgtcgt caattggtat gtgaacgtta tatttgcaaa
    18781 tttgctttac ctttcctcct gaatatccgg tgagaccaat cgtaattgcg cctgcttgat
    18841 ttgcatattc caacgctttc actacatttc ttgaattccc cgaaccggaa attccaatca
    18901 ctaaatcacc ttcttgaaga aagttcttca acggaccaac aaagatttcc tcatagccca
    18961 aatcattggc ataagccatc attgtaggaa tgttatcatt caaacagata aatctgaaca
    19021 ttttttcctt tccataagat attcctttgt tgaaatcgca gcacatgtgt gaagcagtag
    19081 ctgcagaacc tccattcccc attacaaaaa cattctgtcc ttcgtcttta gctttcaaca
    19141 aaatattcat taataaattg atatcttcaa ttgagactct ctcaatggtt tgctttaccc
    19201 gattgaaata ttcacttatg tcttttgtat agttcatgtt aaattaaaag ttatctccaa
    19261 tgtatattat tttagagcca ttattttcaa atgaaaattg taactcattc caatctttca
    19321 ttgattttcg aaaaaaatcc tgtttttcta tcggacaata aaataaaata aaccctgcgc
    19381 cacctgctcc aagcaatttt ccacccannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19501 nnnnnnngga caataaaata aaataaaccc tgcgccacct gctccaagca attttccacc
    19561 caaagcacca gccttgatgc ctcgattgta aatttcatca atatgatcgt ttgatattct
    19621 atctgttaac gtttttttca acatccatcc ttcatgtaaa attcgaccaa cgtcatcaat
    19681 attattattc tcaagtaaag atttcatctg ataagcttgt ttaaccatct ctcgttgaga
    19741 actgaatttt ttcgacccgt tttttatcgc ttgctgctct tttaaaatat cctttgcaga
    19801 atgtttccca ccaatataaa tcatcattaa attatcctgc aattctttct ttttctcagg
    19861 attcataatg attttctcga catttaccgt ttcatcacca taaaaagtaa taaaattcaa
    19921 accaccaaaa gcggcagcat attgatcctg tttcccaatc ggacttccaa ctttatttat
    19981 ttcaatgtca caagccaact ctgcaagttt ctggctacta catgctttat ttaaataagc
    20041 atttaccaca tttaacaaac caaccgtaaa tgaactgctt gaacctaaac ccgttccggc
    20101 tggaatatca gcgattgaat tcaaatcaat acctttaaga ttgtataatg aaagaacttc
    20161 tctgaaaatc ggatgccgaa tctcttcgat tgaatctacc aattcagttt tagaatattt
    20221 caattgaatc ttgcttttat caaaatatgg atgaatggca agatacatgt atttgtcaat
    20281 cgtagtgctt actactgctc cggtttcttc tctgtaaaaa gaaggtaaat cggaaccgcc
    20341 accggcaaat gagattcgaa aaggagtacg tgatatgatc atttttacag tatttgcttt
    20401 aaagtgttca acaattcgta aggtttgttt cgttcaatca tcaacgacat ttttactccg
    20461 gcgtttattc ctgcctttac atcagtttca cgatctccaa tcatgattga atttgtcaaa
    20521 tcgatattcc aatctttagc tgcgtgaaat aacataccag gagcaggttt tctacagttg
    20581 cactgaattt tatattcttt ccgctcttca ggataacccg catcgggatg atgcggacaa
    20641 aaataaatag cattcagata agcctgtttt
//
LOCUS       DAOSCH010000025        20273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1874_length_20273_cov_6.046098, whole genome shotgun sequence.
ACCESSION   DAOSCH010000025 DAOSCH010000000
VERSION     DAOSCH010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 20273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20273
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1874_length_20273_cov_6.046098"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="PLZ54_03370"
     CDS             complement(<1..888)
                     /locus_tag="PLZ54_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3131 domain-containing protein"
                     /protein_id="HOJ66008.1"
                     /translation="MIEKQKYCTLKRKSRKALLSLLLLFIVEITVWGGTQQNFTVPST
                     MKAEAWPAHVELTFENYQCFDYEIFRSKDGGKNFIKCGVTSAGQYLDFLGKPVSKEET
                     FIYRILPKGMDVKNKNAYKFEQKVTVKHSDDEQLLNMVQHYTTRYFYEFAEPVTGMAR
                     ERSNDRNGEIVTTGGTGFGIMALIAGAERNYFSRQDACRSITKIVSFLEKAERFHGAW
                     AHWYDARTGKPFSFSKYDDGGDLVETAFLVQGLLAAREYFNTENVEEHLLSNRISQLW
                     ETVEWNWYTQGTDSLYWHWS"
     gene            complement(902..3130)
                     /locus_tag="PLZ54_03375"
     CDS             complement(902..3130)
                     /locus_tag="PLZ54_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOJ66009.1"
                     /translation="MKNYLSSLVIISFLLMGCTTATQTSTADKKIDAKVDSVLKLMTL
                     DEKIGQMVLYTSDWDVTGPTLKSDYIDDIRSGKCGNIFNAHTVEYVRKLQKIAVEESR
                     LGIPLLFGYDVIHGHKTIFPISLGESASWDLQAIEKSARIAATEAGASGLNWTFAPMV
                     DISVEPRWGRVSEGAGEDPFLGSQIAAARVRGFQGNDLADTTTVLACVKHFAAYGAPL
                     AGRDYNTVDMSQRVFLDVYLPPYKAAIDAGALSVMSSFNEFDGVPATGNKYLLTDILR
                     NQLGFKGFVVSDYTSVNEMVHHGIVANEEEAALLAIKAGLDMDMQGEVYAKYLKGLLE
                     KGLVDEKTIDQSVRRILKVKFKLGLFDDPYRYCNEAREKRTIYAPEHLEAAFDVAKRS
                     MVLLKNDNNVLPLKKGEKIAVIGELAASKRDLLGSWKAAGDWNFMKSVLDELKEYNGE
                     KNVLYAEGCKKMGDDRTGFAQAIATARRADKIVMVIGEDAEWTGEAASRSNINVPGVQ
                     TELLEQLKLLNKPLVVVLMNGRPLVLTKENKLADAILEAWFPGTMGGKAITYVLYGEY
                     NPSGKLTMTFPQNLGQVPIFYYEKNTGRPIYLGNDKYKSKYLDCPNDPLFPFGFGLSY
                     TTFEYSDITLSTKELQQNGSIKATVTVTNTGKTDGEEVVQCYVRDLVGSVTRPVKELK
                     GFEKIFLKAGQSKEVSFTITPDLLAFHRLDMTYGTEPGDFKLFIGGNSRDVKETSFKL
                     ID"
     gene            3548..3757
                     /locus_tag="PLZ54_03380"
                     /pseudo
     CDS             3548..3757
                     /locus_tag="PLZ54_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711510.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
     gene            complement(3955..5610)
                     /gene="hcp"
                     /locus_tag="PLZ54_03385"
                     /gene_synonym="priS"
     CDS             complement(3955..5610)
                     /gene="hcp"
                     /locus_tag="PLZ54_03385"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOJ66010.1"
                     /translation="MFCYQCQETSKGTGCELKGVCGKTSDVAALQDLLIYQLKGLSNY
                     TTALRKVGVESPLVSRFIIDGLFMTVTNVNFDKQRFVEQIREGFDLRSQLKQDLSIHG
                     ITLPASELTEWFADTEADMENMASKVGVLSTENEDVRSLRELIIYGLKGMAAYAKHAM
                     NLRFTDGEINAFVEETLVKTTDDTLSIEELINLVLETGRFGVKVMELLDKANTTAYGH
                     PQVTKVSLQTREKPGILISGHDLKDMEELLKQTEGTGVDVYTHGEMLPAHYYPAFKKY
                     DHFVGNYGGAWWNQTQDFETFNGVILFTTNCIVPPRSNSTYADKVYTTGAAGFPGFRH
                     IAEAPEGKAKDFSSLIEHAKQCTAPIQIEKGEIVGGFAHEQFFALSDQIIEAVKSGQI
                     RKFFVMAGCDGRLKSRNYYTEFAEKLPKDTVILTAGCAKYRYNKLALGEINGIPRVLD
                     AGQCNDSYSLALIALKLKEIFNLNSINELPIVYNIAWYEQKAVIVLLALLYLGVKNIH
                     LGPTLPAFLSPNVAKVLVDNFGIAPIGSVEDDLNMFLNLENFN"
     gene            complement(5670..6026)
                     /locus_tag="PLZ54_03390"
     CDS             complement(5670..6026)
                     /locus_tag="PLZ54_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ66011.1"
                     /translation="MENSEANNRQESKTIPAGEKFVLANQVNYADGSVVSKIIVRNRQ
                     GNITLFAFDKGEFLSEHAAPFNALVVALDGKGTITISGQPFELISGESIILPAGVPHA
                     VNATERFKMMLVMIKD"
     gene            6196..6858
                     /locus_tag="PLZ54_03395"
     CDS             6196..6858
                     /locus_tag="PLZ54_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005866248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOJ66012.1"
                     /translation="MKSIDPEWKSYGSIDEGESSFPADLKYSIKPYRKNEIMFRQGSI
                     CDALYILTSGSVKTEMITETGNIMGIELIKAPRPLAPAFLFSDNNRFPVDVTALEDCE
                     ILRIPKEEVMRLMAIQPDFMQQFLKHNANRAEFLANRLQLLSIKTIKGRIAHFLLEQM
                     AEYGKTFTINRNRTELAEFFGVARPSLARSLSEMVEDNIIKINKKQFTVIDERKLKDL
                     LL"
     gene            complement(6893..9739)
                     /gene="gcvP"
                     /locus_tag="PLZ54_03400"
     CDS             complement(6893..9739)
                     /gene="gcvP"
                     /locus_tag="PLZ54_03400"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOJ66013.1"
                     /translation="MKDFLSFRHIGVSSDEIQEMLEVVGAESLDELITQTIPLSIRLN
                     EKLNLPSALTENQYAQHISEIAEKNKIFTSYIGRGWYDTCTPAVILRNVFENPSWYTS
                     YTPYQAEISQGRLEALLNFQTAVCEFTGLPLANASLLDEATAAAEAATMMYNLRSREQ
                     MKNGANLLFVDENIFTSTLAVLQTRTQAQGIQLEIGDYKSVQFSEKHFGAILQYPNAD
                     GNVEHYFSFVSQAHTAGCKVAVAADILSLALLVPPGEWGADIVFGSTQRWGIPMGFGG
                     PSAAYFATRDEFKRDMPGRIIGISKDKNGKPALRMALQTREQHIKREKATSNICTAQA
                     LLASMAGFYTVFHGPEGIRNIAKNIHAIAAYINAMLPTYGYRQLNKLFFDTLKIVLPE
                     NVSQEEFRNIALSHKVNFYYFETGEVGLSINETTEAPDIQRLINIFSEAVHKKPIVFN
                     PTDLSEFEIPDEKIRRKSAYLTQEIFHRYHTEMEMMRYIKKLERKDISLTHSMISLGS
                     CTMKLTAASEMLPLSKAEFLKIHPLAPSDQVKGYIELIGELEKYLCVITGFDACSLQP
                     NSGAAGEFAGLMTIKAFYRSRGETERNVVLIPASAHGTNPASAHQAGLEIVVVDCDEN
                     GNIDLNDWKVKAETYRTRLLGCMVTYPSTHGIFEAHIGEMCQIIHANGGQVYMDGANM
                     NAQVGLTHPAKIGADICHLNLHKTFAIPHGGGGPGVGPVCAAAHLAPFLPGKTSGQNT
                     VSAAAYGSASILPITYGYIRMMGAEGLTNASKIAILNANYLAERLKETYGVVYRGENG
                     RVGHELILECRNFKVAGITETDIAKRLMDYGFHAPTLSFPVHGTLMVEPTESESKAEL
                     DRFVEAMIQIYEEIMEIENGTADKTDNVLLNAPHPEYEMVSDEWKHLYSRKKAAYPTK
                     WTAENKFWINVARIDNAFGDRNLICSCPPTVEWQ"
     gene            10500..11612
                     /locus_tag="PLZ54_03405"
     CDS             10500..11612
                     /locus_tag="PLZ54_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HOJ66014.1"
                     /translation="MIDTLNLFGKTKIEVPKTEGIKYAGSKLKILPYIFNMISGLDVE
                     NILDGFSGTTRVSQALAKTGFKTTSNDISIWSATFAKAYLLNNRKPEYYLPIIEYLNS
                     LKGYVGWFSEYYGGTENNKEKRPFQLKNTMKLDAIRDEIDRMNLDEIEKSVALTSLIL
                     ALDSVDNTLGHYAAYLAEWSPRSYRDLKLKLPLLFKNNQQNEVIKGDIFETVKNHKFD
                     LAYFDPPYGSNNGKMPPSRVRYNSYYHIWTTVILNDKPKLFGKVNRRLDSKDEVAASV
                     FEEFRKDEQGHFIAMNAIKELIHCTNSKYILLSYSSGGRATKEELFDIINSEGKMIQA
                     IEINYKKNVMGQMRWTNEWINSDDKYMEYLFLMEKC"
     gene            11707..13029
                     /locus_tag="PLZ54_03410"
     CDS             11707..13029
                     /locus_tag="PLZ54_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOJ66015.1"
                     /translation="MITSEPIIHVGILSSREIAFILNGIFIISDGKNFSGKQKAEWKN
                     GKILFDGETFDELNFQPHDESSTFDLLDVVIGIHFHWERKENQRFKGALKIIVENEQL
                     TVVNEIKAEDYLLSVISSEMSATSSLELLKAHAVVSRSWLFYPLQKDKKELSGRESVE
                     ENTENRHIKWYERGEHEHFDVCADDHCQRYQGITRATSDAVRHAVEATRGEVLMFEQQ
                     ICDARFSKCCGGVTETFDNCWAPEEHPYLSSIVDNDLLPQSFDLDLTMEENAQKWIRE
                     FPTAFCNTQDKNVLTQVLNNYDQETTDFYRWKVVYTQEELAEILRRRSGYDFGDILDL
                     IPLKRGKSGRIVELKIVGTKRTMIVGKELEIRKWLSDSHLYSSAFVVDKENYSQQIPG
                     KFILTGAGWGHGVGLCQIGAAVMGEKGYAYERILLHYFRGAELKRIYE"
     gene            13516..14646
                     /locus_tag="PLZ54_03415"
     CDS             13516..14646
                     /locus_tag="PLZ54_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOJ66016.1"
                     /translation="MKKIPFLLLPCFVLFIACTHGSSHLAKEQPVDSLRLSHAEGFSI
                     RYFRNYKEVVVFNPFSHNAVYQRYYLVRDTDVSVPADGLKIKIPVRSLAATSVTHFEF
                     LNLLHVIDAVTGVCSSKIIYSPQINERLNKGLIADLGDAFNINLEKTVQLHPDLVMVS
                     GYNQNDPFAQRLLQAGIFPVYNNEWMEKSLLGRAEWIKFLAAFFDREKIADSLYLEIE
                     KEYQNTANRVKGIKFRPQIMSGSNFRGTWYMPGGRNFMAQLFSDAGGNYFYARDTTVG
                     SLPLSFEKVLKNFSESEVWLNCNYASLSELLQADPRHALFRPVKLQQVYNFNKRMLPS
                     GSNDFWESAVAHPDLLLKDVIFILHPELLPGYEPFYTQKLNP"
     gene            14653..15648
                     /locus_tag="PLZ54_03420"
     CDS             14653..15648
                     /locus_tag="PLZ54_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOJ66017.1"
                     /translation="MHSKNHLLFFAFSLLVVFLFLADLAWGSVAIPAKDIFSVLCGQQ
                     SSSIYTEIIVNFRLPKAITAILAGASLSVAGLMMQTLFRNPLADPYILGVSSGASLGT
                     ALVVMASGFLPFSFVGNGWVLIISAIVGAALVLLIVLFVSFRVNDIVSLLIVGIMFGT
                     IAAALVSIIQNFSNPDAIKLFLMWTFGSLSAVTWNYLQILLPVVLVGIGIAFFLQKRL
                     DGLLLGENYARGLGIPISQTRSLIILATGLLAGGITAFTGPIAFVGVAVPHIARGIFQ
                     TSSHKILLPACMLVGAALLMICDIITQIPDYTLPVNTISALFGAPVIIWIILRRK"
     gene            15676..16665
                     /locus_tag="PLZ54_03425"
     CDS             15676..16665
                     /locus_tag="PLZ54_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ66018.1"
                     /translation="MSVLSTQHLSIGYRRKGTANVIQHDLNLKLESGKLVGLIGPNGS
                     GKTTLLRTIAGLLKPIDGNILIDDKEFLLLSQREKALNVSFVLTEKVDIPNADVFDMV
                     SFGRFPYNNWRGNLSKQDRTKVFQALQMVRMEKMMHRYINELSDGERQRVMIAKALAQ
                     DTPIILLDEPTAHLDVPNRVGIMLLLHKLAHTTGKMILLSTHELDLALQAADLIWLMS
                     ENGGIECGVPEDLVFSGSFNRTFASDYYFFNEANGNFSLNYPLTGKVSVSGNKMRIYW
                     TLRALARAGYEAVENAPLKIEVTDSAWILNQKEIFSVAEMLDELNNLNGNTSL"
     gene            17052..17249
                     /locus_tag="PLZ54_03430"
     CDS             17052..17249
                     /locus_tag="PLZ54_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOJ66019.1"
                     /translation="MSKKLTRSSNQMIAGVCAGIAEYFGWNVTLVRVIYVLLSIFSAG
                     FPGTMVYIILWIVMPRPEKFD"
     gene            17323..19341
                     /gene="uvrB"
                     /locus_tag="PLZ54_03435"
     CDS             17323..19341
                     /gene="uvrB"
                     /locus_tag="PLZ54_03435"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOJ66020.1"
                     /translation="MNFKLESPYQPTGDQPEAIQQLVEGINAGIPYQTLLGVTGSGKT
                     FTIANVIEQVQRPTLVLSHNKTLAAQLYSEFKSFFPHNAVEYFVSYYDYYQPEAYLPL
                     TDTYIEKDLSINQEIEKLRLSATSSLLSGRRDVVVVSSVSCLYGIGNPDDFSKNVIEI
                     RKGQHLVRNAFLRNLVNSLYTRNEIELNRGNFRVKGDTVDIFLAYTDFILRIVFWGDE
                     IDEILTVDPVNYDIIDSLDAYKIYPASIFVTTKERIAVAINEIEKDLQLQVDYFRSIG
                     KDLEAKRIYERVRYDIEMIKELGYCSGIENYSRYFDGRPPGSRPFCLLDYFPKDFLLV
                     IDESHVTIPQIRAMYGGDASRKQNLVDFGFRLPAAKDNRPLRFEEFEQLTPQTIYVSA
                     TPADYELVKSEGIVVDQLIRPTGLLDPVIEVRPSLNQIDDLLEEIAQRTENNERVLVT
                     TLTKRMAEELTNYLSKMGVKCNYIHSDVETLDRVQIMEDLRRGVYDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSHRSLTQTAGRAARNLNGKVIMYADQITESMEKTINET
                     NRRREKQLRYNEAHHITPTAIVKGERNPLSKAEPYAYVEPEKSTDIAADPVVRYMSVP
                     ALEKAIAKTRKAMQEAAKKLEFLEAAQLRDELMKLEELLKKKMFEAKN"
     gene            19520..20140
                     /locus_tag="PLZ54_03440"
     CDS             19520..20140
                     /locus_tag="PLZ54_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOJ66021.1"
                     /translation="MLEIIPKGILIGLCISVPVGPIGMLVIQRTLNRGRKYGVATGLG
                     ASMSDLFYTIITLFFLSFVISFIEAHRFVIELIGSLVVICFGYFIYKNNPSVQPKPNE
                     TVQHNVYRDFISSFVLTFSNPLILFILIALFARIGFIDSETSFFQTIIGIISILIGAT
                     IWWLTLTFIVSHFRSKIGMHQLKAINRITGGLIALIGCIGLVSSLI"
BASE COUNT         6178 a         3846 c         3937 g         6312 t
ORIGIN      
        1 cgaccaatgc cagtaaagcg aatcggtacc ttgcgtatac caattccatt ctactgtttc
       61 ccaaagttgt gagatgcgat tcgacaacaa gtgttcttca acattttcgg tgttgaaata
      121 ttcccgtgca gccagcagac cttgtaccag aaatgcggtt tctaccaaat cgcccccatc
      181 atcatatttt gaaaagctga agggttttcc cgttcgtgcg tcataccaat gcgcccatgc
      241 tccgtgaaat ctctcagctt tttccaaaaa ggaaacaatt tttgtgatgg aacgacaggc
      301 atcctgccga ctgaaataat ttcgttctgc accggcaatc aatgccataa taccaaaacc
      361 agttccgccg gtagttacaa tttctccatt tcggtcattg cttctttcgc gcgccatgcc
      421 ggtaacaggt tcggcaaatt cataaaaata tcttgttgtg tagtgttgta ccatgttcag
      481 taattgttcg tcatcactat gctttacggt tactttctgc tcaaatttgt atgcgttttt
      541 attttttaca tccattcctt ttggcaaaat tctgtaaata aaagtttctt cctttgaaac
      601 tggtttgccc aaaaaatcta gatactgacc tgcagaagtt actccacatt tgataaaatt
      661 ttttcctcca tctttcgacc tgaaaatttc gtaatcgaaa cactgataat tctcaaaggt
      721 taattccacg tgtgcgggcc acgcttctgc tttcattgta gaaggtactg taaaattttg
      781 ttgagtgcca ccccacactg ttatttcaac gataaataat aacaataatg aaagtagcgc
      841 ttttctactt ttccttttca acgtgcaata tttctgcttt tctatcatga aaaaagattt
      901 tctaatcaat tagtttgaat gaagtttctt ttacgtctct cgaatttcca ccaataaaca
      961 gtttaaaatc tcctggttcg gttccgtaag tcatatccag tctgtgaaaa gccagcaaat
     1021 cgggcgtaat ggtaaaagaa acctctttac tttggccggc tttcagaaaa attttctcaa
     1081 aacctttcaa ctccttaaca ggacgggtta cacttcccac caaatcgcgc acatagcatt
     1141 gcactacttc ttcaccgtct gttttaccgg tattggttac ggtaacagtt gccttgatgc
     1201 ttccgttttg ttgcaattct ttggttgaca aagtaatgtc tgaatattca aaagtggtgt
     1261 agctcaatcc gaaaccaaaa ggaaacagcg gatcatttgg gcaatcgaga tattttgatt
     1321 tgtatttatc gttgcccaaa tatatgggac gtccggtgtt tttttcgtaa taaaatatgg
     1381 gaacttgacc taaattttgt ggaaaagtca tggtcagttt gccggaagga ttatattctc
     1441 cgtaaagaac ataagttatg gcttttccac ccatcgttcc gggaaaccaa gcttccagaa
     1501 tagcatctgc caatttgttt tcttttgtga gaacaagagg acgaccgttc atcaatacta
     1561 caacgagagg tttatttaac aatttcagtt gctcgagtaa ttctgtttgc acacccggca
     1621 cattgatatt cgatcggctg gcagcttctc cggtccattc ggcatcttcg ccaatcacca
     1681 tcacaatttt atcggctctg cgggctgtag ctattgcttg agcaaatccg gtacggtcgt
     1741 cccccatttt tttgcagcct tctgcgtaga gtacattttt ttctccattg tattctttca
     1801 attcatccag aacagatttc atgaaattcc aatctccggc agctttccac gaaccaagca
     1861 aatcgcgttt tgaagccgcc aattcgccga ttactgcaat tttttctcct tttttaagag
     1921 gaagaacgtt gttgtcattt ttcagtaaaa ccattgaacg tttagcaacg tcaaaagcag
     1981 cttccagatg ttcgggggca taaatcgttc gtttttcgcg cgcttcgttg caataacgat
     2041 aagggtcatc aaaaagaccc aatttgaatt tcacctttaa aattcgacgt actgactgat
     2101 caattgtttt ttcatctacc aagccttttt cgagcagtcc ttttaaatat ttggcataca
     2161 cttctccctg catatccata tcgagaccag ccttgatggc cagcaacgca gcttcttcct
     2221 cattggcaac aatcccgtga tgaaccattt cgtttaccga agtataatca cttactacaa
     2281 agcctttgaa tccgagctga tttctcaata tgtcggttag caaatatttg tttcccgtag
     2341 caggaacacc gtcaaattca ttgaaagaac tcataacgct cagagcacca gcatcaatgg
     2401 cagctttgta aggaggcaga taaacatcaa gaaatacgcg ttgcgacatg tccactgtgt
     2461 tataatcgcg tcctgccaac ggtgctccat aagcagcaaa atgttttaca caggctaaaa
     2521 ctgttgtcgt atcggctaaa tcatttccct gaaaacctct gacacgggcc gcagcgattt
     2581 gagaacccag aaaaggatct tcgcccgccc cttcagaaac ccttccccag cgcggctcta
     2641 cagaaatgtc aaccatgggc gcaaaagtcc agtttaatcc ggatgcacca gcttcggtgg
     2701 ccgcaatacg tgctgatttt tcaatagctt gcaaatccca cgaagccgat tcacccaaag
     2761 aaataggaaa aatagttttg tgcccatgaa tgacatcgta accaaacaac aacggaatac
     2821 ctaaccgtga ttcttctacg gcaatttttt gaagtttgcg tacatattca accgtatgtg
     2881 cgttgaagat gttgccacat ttgccgctgc gtatatcgtc tatgtaatcg ctttttaatg
     2941 ttggaccggt aacatcccaa tcactggtat aaagcaccat ttgtccgatt ttctcatcca
     3001 aagtcatcaa ttttaataca gaatcaactt tggcatcgat ttttttgtca gctgtggaag
     3061 tttgagttgc agtagtacaa cccataagca ggaaagaaat aattaccaat gatgaaaggt
     3121 agtttttcat ggttcaacgt tttaaaattt cgatttcaaa taacgttgca aagaaggaga
     3181 taattaaaag cgtttgcaaa tttttatacc ctataacaac cccactggtg aaaatatttt
     3241 taatttatac ccctattttt cccctataat gagaaaatat tattatttaa acctttaaaa
     3301 aacaaataaa tatattttat tttaatttta aaaaatattt tgtagaattg cttgaattaa
     3361 ggcaaactat aacctaaaaa tttgttaaat tatggaaagg gagaagaata aatgagtgaa
     3421 acaatatctg ttgagtgtaa tttgacaatt tctaaactga tattagctta taaaaaaaac
     3481 cgagcagtaa aaaataatag ttatttttac aactgcgatg gaaaagagaa aaaatttgac
     3541 atcaaaaatc aaggctgaaa ttgttttatc cttgttgcca cagggaaaag tcgaagctgc
     3601 aagacgcgta aaagcaatgg ttgcaaaaat ggacgaactc ggtttcggca attgcaccaa
     3661 taccagagct tgcgaggccg aatgcccaaa aaatatttca atctccaaca ttgcccgcct
     3721 caaccggcaa tttctttcag ccaaattcaa ggattgacat tctcgtttat tactcgatag
     3781 atttcaaaaa agaccgtaag aaaaacttgc ggtctttttt gttgaaaaaa tgaaaattac
     3841 atttgtaatt tccttaaaat tttctatatc ccatttatac tgaacgggta atcaaaaaaa
     3901 tttaggtagc tccaaaaaca agattttgcc gaataataaa actttgtgaa ataatcaatt
     3961 gaaattttct aaattgagga acatattcaa atcgtcctcc acgctgccaa taggagcaat
     4021 tccaaaatta tctaccaaca ctttggcaac atttggtgaa agaaatgccg gcaatgtggg
     4081 tcccaaatgg atatttttca ctcccagata aaggagcgca agaagcacaa ttacagcttt
     4141 ctgttcgtac catgcaatgt tatagactat gggcaattca ttgatactgt ttaaattaaa
     4201 aatctctttc agtttcaggg ctatcagtgc cagcgaataa ctgtcgttac actggccggc
     4261 atcgagcact cgcggaatgc cattgatttc tcccaatgcg agtttgttat agcgatattt
     4321 tgcacagcca gccgtaagaa taacagtatc ttttggcaat ttttccgcaa attccgtata
     4381 ataattgcga ctttttaatc ttccgtcaca accggccatt acaaaaaatt tcctaatttg
     4441 tccggatttc actgcctcga taatctgatc cgacagagca aaaaattgct cgtgagcaaa
     4501 accgcccaca atttctcctt tttcaatctg tatgggagcg gtacattgtt tggcatgttc
     4561 aatcaacgac gaaaaatcct ttgcctttcc ttcaggtgct tcggcaatat gtctgaagcc
     4621 tggaaaacca gccgctccgg tagtataaac tttgtcggca taagtggagt tgcttctcgg
     4681 tggaacaata cagttggttg taaaaagaat cactccgtta aatgtttcaa aatcctgtgt
     4741 ttggttccac catgctccgc cataatttcc gacaaaatgg tcgtattttt taaatgccgg
     4801 atagtagtgt gccggcaaca tttctccatg tgtataaacg tcaacgcctg ttccttcggt
     4861 ttgtttcagc agctcttcca tatctttgag gtcatgtcca ctgattaaga tacccggctt
     4921 ttcccgagtt tgcaaactga cttttgtaac ttgtggatgt ccataagcag ttgtattggc
     4981 tttatcgagc agctccatga ctttgactcc gaaacgtccg gtttccaaaa ccaaatttat
     5041 cagctcttct atactcaatg tatcgtctgt ggtttttacg agtgtttctt ctacgaaagc
     5101 atttatttcg ccgtcggtga aacggagatt catggcatgt ttggcataag cagccattcc
     5161 tttcagtcca taaattatta gttcgcgcaa agagcggacg tcttcatttt cggtactcaa
     5221 cacgccaact ttgcttgcca tattttccat atctgcttcg gtatcagcaa accactccgt
     5281 gagttcggac gccggcaatg ttatcccatg aattgataaa tcttgtttta attgagaacg
     5341 cagatcgaat ccttcacgaa tttgttcaac aaatcgctgt ttatcgaaat tgacattggt
     5401 aaccgtcata aaaaggccgt cgatgataaa acgactcaca agcggacttt caactcccac
     5461 tttgcgcaaa gcggttgtgt aatttgataa tcctttcagc tgataaatca acaaatcctg
     5521 caaagccgcc acatcagaag ttttaccaca aacacctttc aattcgcagc cggttccttt
     5581 agaagtttcc tgacactgat aacaaaacat attcattttt ttcacaatta attttaaaat
     5641 ctcaaaatct atttttattt acttgttttt caatctttta tcattaccaa catcatttta
     5701 aaacgttctg tggcattcac cgcatgtgga acaccggcag gtagaatgat actttctccg
     5761 gaaattaatt caaacggctg ccccgaaatg gtaatcgttc ctttaccatc gagagctacc
     5821 accaatgcat tgaatggtgc tgcatgttca cttaaaaatt ctcccttgtc gaaagcaaac
     5881 agtgtaatgt ttccctgacg atttcgaaca atgattttgc ttaccaccga gccatcggca
     5941 tagttgacct gattggccaa tacaaacttt tcgcctgcag gaattgtttt gctttcctgc
     6001 ctgttgttag cttctgaatt ttccatcatg ttttgaattt ttagttatgt aattttataa
     6061 attgtttttg aaattgaagc acaatacttt atatttgtac actgtacgag attgaagttt
     6121 ttgacattgc aaagttagag agtttaaaac aattaaaatg taacacttgt tacagatgaa
     6181 aaaaattgat ttttcatgaa aagcattgat cctgaatgga agagttacgg cagtattgat
     6241 gagggagaaa gtagctttcc tgccgattta aaatacagca ttaagccata ccgaaaaaac
     6301 gaaattatgt ttcgtcaggg aagtatttgc gatgcattgt acattttgac ctcgggttcg
     6361 gtgaaaacag aaatgattac cgaaaccggc aatattatgg gaatagaact gataaaagct
     6421 cctcgtccgt tggctccggc ttttttattt tccgacaaca accgttttcc cgtagatgtt
     6481 actgcactcg aagactgcga aatcctacgt attcccaaag aagaagtaat gcgtctgatg
     6541 gcgatacagc ccgattttat gcagcagttt ttaaaacaca atgccaaccg agcagagttt
     6601 ttagccaatc gacttcagtt gctttccatt aaaaccataa agggacgcat tgctcatttt
     6661 ttattggaac aaatggccga atacggaaaa actttcacca tcaatcgaaa caggaccgaa
     6721 ttggcagaat tttttggcgt agctcgtcct tcattggcca gaagtctttc ggaaatggtc
     6781 gaagacaaca tcattaaaat caacaaaaaa cagtttactg taattgatga gcgcaagtta
     6841 aaagatttgc tcctttaatg ttaaaaactt aataaattgc gacaataaaa atttattgcc
     6901 actcaacagt tggaggacag gaacaaatta aattcctatc accaaaagca ttatcaatac
     6961 gtgcgacatt tatccaaaat ttgttttcag cagtccattt cgtaggataa gcggcctttt
     7021 ttctgctgta aagatgtttc cactcatccg acaccatttc atattccgga tgaggtgcat
     7081 tcaataaaac attgtccgtt ttgtcggctg tcccattttc aatttccata atttcctcat
     7141 aaatttgaat catagcttcc acaaacctgt cgagttctgc ttttgattca ctttcggttg
     7201 gctcaaccat caacgtaccg tgaacaggaa aagacaaagt aggagcatga aatccataat
     7261 ccatcaaacg tttggctata tcggtttccg taataccggc aactttaaaa tttcgacact
     7321 ccaaaatcaa ttcgtgcccc acgcgaccat tttcgccccg atacacgact ccgtaagttt
     7381 cctttaaacg ttcggccagg tagttggcat ttaaaatagc aattttagaa gcatttgtca
     7441 aaccttcagc tcccatcatt cgtatataac catacgtgat gggcaaaata ctggcactgc
     7501 cgtaagctgc ggctgaaaca gtattctgac cggatgtttt acccggcaaa aaaggtgcca
     7561 aatgagcagc agcacaaacg ggtccaacac cgggaccgcc tccaccgtga ggaatggcaa
     7621 aagttttatg taaattcaaa tgacaaatat ccgcaccaat ttttgccgga tgagttaatc
     7681 cgacctgagc attcatattg gctccatcca tataaacttg tcctccgttt gcatgaataa
     7741 tttggcacat ttccccaatg tgagcttcaa aaatgccatg tgtagaagga taagttacca
     7801 tacaacccaa caatcgcgta cgataagttt ctgctttcac tttccagtcg ttgagatcaa
     7861 tattcccatt ttcatcgcaa tcgaccacaa ctatttcaag ccccgcctga tgagctgaag
     7921 ccggattggt gccatgagcc gatgccggaa ttaaaacaac gtttcgttct gtttcaccac
     7981 gcgaccgata aaaggctttg atagtcatca agccggcaaa ctctccggct gcaccggaat
     8041 tgggctgcaa actgcaagca tcaaaaccgg taataacaca caaatatttt tccaattcgc
     8101 caattagttc aatgtaacct ttcacttgat cggacggagc caaaggatga atcttcaaaa
     8161 attctgcttt gctcaacggt agcatttctg aagctgccgt gagtttcatg gtgcacgacc
     8221 ccaatgaaat catggaatga gtcaacgata tatcttttcg ttccagtttc ttgatataac
     8281 gcatcatttc catttcggta tggtagcggt gaaaaatttc ctgagtcaga taagccgact
     8341 ttcgtctgat tttttcatcc ggtatttcaa actccgacaa gtcggttgga ttgaagacaa
     8401 tcggtttttt atgaacggct tccgaaaaaa tatttatcaa tctttgtata tcgggtgctt
     8461 cggtagtttc atttatactc aaacctactt ctccggtttc aaaataataa aaattaactt
     8521 tgtgggagag cgcaatgttt cgaaattctt cttgagaaac attttcgggc aaaactattt
     8581 tcaaagtatc gaaaaaaagt ttattcaact gacgataacc gtaagttggc agcattgcat
     8641 tgatataagc tgctatggca tgaatgtttt tcgctatatt tctaattccc tccggcccgt
     8701 gaaaaacggt ataaaaaccc gccatcgaag ccaaaagagc ttgtgcggta caaatgttag
     8761 aagttgcctt ttcgcgtttg atgtgctgct cacgggtttg aagtgccata cgcaaagccg
     8821 gtttcccgtt tttatccttg ctaatgccaa tgatgcgacc cggcatatcg cgcttaaatt
     8881 catcgcgtgt agcaaaataa gccgccgaag gacccccaaa ccccatagga attccccaac
     8941 gttgagtaga accaaaaaca atatcggcgc cccattctcc cggtggaaca agtaaagcca
     9001 aacttaaaat gtcggcagcc acagcaactt tacaaccagc tgtatgtgct tgtgatacaa
     9061 atgaaaaata atgctctaca ttcccatccg cattaggata ttgaagaatc gctccaaaat
     9121 gtttttctga aaattgtacg gatttataat ccccaatttc gagttgtatt ccctgtgctt
     9181 gtgtccgcgt ttgtaaaaca gccaaagttg aagtaaaaat attttcatcg acaaaaagca
     9241 aatttgctcc atttttcatt tgttccctcg aacgcaaatt atacatcatg gtagctgctt
     9301 ctgctgcggc agttgcttca tcgagcaaag aagcatttgc caatggaaga ccggtaaatt
     9361 cacaaacggc ggtctgaaaa ttcagcaaag cttccaatct tccttgagaa atttcggctt
     9421 gatagggagt gtaggaagtg taccatgaag gattttcaaa aacattccgt aaaattacgg
     9481 ccggtgtaca ggtgtcatac catcctctgc caatatagga agtaaaaatt ttattttttt
     9541 ctgcaatttc agaaatatgc tgagcatact gattttcagt cagagcagaa gggagattca
     9601 atttttcgtt caaacgaatg gagagaggaa tagtttgagt gataagttca tctaaacttt
     9661 cagcccctac aacttccaac atttcttgaa tttcatcaga cgaaacgcca atgtgtcgaa
     9721 aagagagaaa atctttcatt tgattatata ttactgattt ttagttaatt ataaaaattt
     9781 gaatgatcga atattaaaaa ataaagcggc taaaatttca agttgtaaag ttacagcaaa
     9841 aaaatcagga aagaaaattc aattttttaa caaaatattg tttgagtcca ctccgaaaag
     9901 tttttcaaag agaattagtt tttaaagaca ttggaagtac ttgaccattt gtagagaagg
     9961 tttgtgccgt tggtgcttat gacagtgaga aaaatcaaga atttgcaacg acaaacaaca
    10021 aagaacttat ttcaagtggt atacagggag ccaaaggaag atatgaactt tcagttgatt
    10081 cacaaaatcc tgacaattta atagtaacca atacccagac aggagaaatt ataaaagcac
    10141 aaaaataaaa acaaaaagaa gagtggtaat ctacaatggc gtatcaaaac agagcatggc
    10201 aactatcgtt attttaacct tgagcaggtg caaatttttc ttgcttcttt tcaaaaaaaa
    10261 atctcatttt ttatcgtttt tgaaaaaaat taggttttaa aaaaaaggac tcaatactta
    10321 atttaggtca gctttacaaa tagacaataa ttcaatacgt tttattaaat ttgcaaatca
    10381 aacttcgatt ataagtaaaa aaacatggat tcttagaatg attttaaaca acttttcaaa
    10441 cttttggcag acaaaaaggt actgagttgt taattttcta agcaatattc acgatgataa
    10501 tgattgatac attaaattta tttggtaaaa ccaaaataga agttccaaaa actgaaggaa
    10561 ttaaatatgc tggttcaaaa ctaaaaattc ttccatatat ttttaatatg atttctggct
    10621 tagatgtaga aaatatatta gatggattca gtggaacaac aagagtatct caagcacttg
    10681 ccaaaacggg ttttaaaact acttcaaacg atatatctat atggtcagca acttttgcaa
    10741 aagcatattt gctaaataat agaaagcctg aatactattt gcctattatt gagtatttaa
    10801 attcattaaa aggttatgta ggttggttct ctgagtatta tggaggcact gaaaataata
    10861 aagaaaaaag gccttttcaa cttaaaaata caatgaaact ggatgcaatt agagatgaga
    10921 ttgatagaat gaatttagat gaaatagaaa agtctgtagc tctaacaagt ttaattctcg
    10981 ctcttgattc agtagacaac acattaggcc attatgctgc atatttagca gaatggtctc
    11041 ctagatcata tcgtgattta aaattaaaat taccgttatt atttaaaaat aatcagcaaa
    11101 atgaagtaat aaaaggagat atttttgaaa cagtaaaaaa tcataaattt gatcttgctt
    11161 attttgatcc tccttatggt tcaaataatg gaaaaatgcc accaagcaga gttagataca
    11221 attcatatta tcatatctgg acaactgtta tattgaatga caagccaaaa ctttttggaa
    11281 aggttaatag aagactggat tcaaaggatg aggtagcagc gtcagttttc gaagaattta
    11341 gaaaagatga acaaggtcat tttattgcaa tgaatgcgat aaaagaattg attcattgta
    11401 caaactctaa atatattctt ttatcgtata gttcgggtgg tagagctaca aaagaagaac
    11461 tttttgatat aataaattct gaaggtaaaa tgattcaagc aattgagata aattataaga
    11521 aaaatgttat gggtcaaatg agatggacaa atgaatggat aaatagtgat gataaatata
    11581 tggaatacct ctttttaatg gaaaaatgtt gattccactc acttcaaact tattgttatt
    11641 cttatattga gaaaaatttt ttgaaagaat tgaaagccta aaatagatta aaaacaaatc
    11701 atagaaatga ttacctcaga accaattatt catgtgggga tattaagttc ccgggaaata
    11761 gcatttattc taaatggcat ttttattatt tccgatggaa aaaatttttc cgggaagcaa
    11821 aaagccgaat ggaaaaatgg aaaaatactt tttgatggcg aaacttttga tgaattgaat
    11881 tttcaaccgc atgacgaatc gtcaacattt gatttactag acgtagtgat cgggattcat
    11941 tttcattggg aaagaaaaga aaatcagcga tttaaaggag ctttgaaaat tattgtggaa
    12001 aatgagcaat taacagttgt caacgaaata aaggccgaag attatctgct cagtgtaatt
    12061 tccagtgaaa tgagtgctac ctcctcactt gaactgctga aagcccatgc cgttgtttcc
    12121 agaagctggc tgttttatcc tctgcaaaaa gacaaaaaag agctttccgg tcgggaatcg
    12181 gtcgaagaaa acacggaaaa tcgacatatc aaatggtacg aaagaggcga acacgaacat
    12241 tttgatgtgt gtgccgacga tcattgtcag cgttatcaag gaattacaag agcaacttcg
    12301 gatgcggttc gacatgcagt agaagccacg cgtggcgaag tgctgatgtt tgagcaacaa
    12361 atatgtgatg ctcgtttttc caaatgctgt ggcggagtta ccgaaacttt tgacaattgc
    12421 tgggcgcccg aagagcatcc ttatttatcc agtattgtgg acaatgattt attacctcaa
    12481 agttttgatt tggatttaac gatggaagaa aatgcccaaa aatggattcg tgaatttccg
    12541 acagcttttt gcaatacgca ggacaaaaat gttctcacac aggtgttgaa taattacgat
    12601 caggaaacaa cagattttta tcgctggaaa gttgtttaca cccaggaaga acttgccgaa
    12661 attttgcgac gtcgttcagg atatgatttc ggagatattt tagacttgat tcctttgaaa
    12721 cgaggtaaat cgggccgaat tgtggagtta aaaattgtgg gaaccaaacg aacgatgata
    12781 gtaggtaaag agttggaaat tagaaaatgg ctgtccgata gccatttgta tagctcggct
    12841 tttgtagttg acaaggaaaa ttattcccaa caaattccag gaaaatttat tttgaccggt
    12901 gcgggttggg gacatggcgt aggactttgt caaattggtg ctgccgttat gggcgaaaaa
    12961 ggttatgcct acgaacgtat tttgctgcat tattttcgag gcgctgagtt gaaaaggatt
    13021 tatgaatgaa gaatagtatt tttagcattt tcttgattcc atttcatcag aatataattt
    13081 attttttaat ggtctgatgg aatccacatg tctaaatgtt atattcattc gtgtttgtgt
    13141 atgtaataca tgtgagtttc ttaattgaaa ataataaata aattttgagt ccatattttt
    13201 aatattttgt ataaatttac ttaattgatt aataatcaat tgtttaaaaa aaataacata
    13261 atctgtataa atggttgatt gtaagtataa tttcaatatt gatgtcagag aatattcgag
    13321 aataaaaaat ttttcacttt tttagtttac tctatttcaa tagtttgata aagtaatata
    13381 ctttcattca ttggtcattt tcagtttttc aaactatctt tgcaagcggt taatccttcg
    13441 gaaaagtttt ttggaaaaaa tgaatttcaa gacaatgaaa attatcaaag gcttttccga
    13501 taatttggta aaacaatgaa aaaaattccg tttttgttgc ttccctgttt tgttttgttt
    13561 atcgcatgta cacacggaag ttcgcatctg gcaaaagagc agccggtgga ttcgcttcga
    13621 ctttctcatg ccgaaggttt ttccattcgt tattttcgaa attataaaga agtggttgtt
    13681 tttaatcctt tttcgcacaa tgctgtttat caacgttatt atttggttcg ggatactgat
    13741 gtgtctgtgc cggcggatgg cttgaaaatc aaaattcccg ttcgttcgtt ggcagcaaca
    13801 tcagttaccc actttgaatt tttgaatttg cttcatgtga ttgacgctgt aacgggtgtc
    13861 tgttcttcaa aaattatcta cagtccgcaa attaatgagc gtctcaacaa aggcttgata
    13921 gccgatttgg gagatgcatt caatatcaat ctcgagaaaa cggttcagtt gcatcccgat
    13981 ttggtcatgg taagcggcta caatcaaaac gacccttttg cacagcggtt gcttcaggcc
    14041 ggaatttttc cggtttacaa caacgaatgg atggaaaaat cgctgttggg gcgcgccgaa
    14101 tggataaaat tcttggcggc tttttttgac agagaaaaaa ttgccgacag tctttatctg
    14161 gaaattgaaa aggaatatca aaatactgcc aacagggtaa aagggataaa atttcgtccg
    14221 caaataatgt ccggttccaa ttttcgtggc acttggtaca tgcccggagg acgaaatttt
    14281 atggcacaac ttttttcgga tgccggtgga aattattttt atgcccgcga tacaaccgta
    14341 ggaagtttac cgctcagttt tgaaaaagta ctgaaaaatt tttctgaatc cgaagtttgg
    14401 ctaaactgca attatgcttc attgtccgaa ttgttgcagg ccgatccgag acatgctctt
    14461 ttccgtcccg taaagttgca acaggtgtat aatttcaata aacgcatgtt gccatctggc
    14521 tccaacgatt tttgggaaag tgcggtagct catcccgatt tacttttgaa agatgtgatt
    14581 tttattttgc atcccgaatt gctgcccggt tacgaaccat tttacactca aaaattaaat
    14641 ccgtaaggtt gtatgcattc gaaaaaccat ttgctttttt ttgctttttc gttgctggtt
    14701 gtttttttgt ttctggccga tttggcatgg ggaagtgtag ctattccggc aaaagatatt
    14761 ttttcagtgc tctgcgggca acagtcttcc tcaatttata cggaaataat tgttaatttc
    14821 cgcttgccaa aggccattac ggccatactt gccggcgctt cgctttcggt agccggcctt
    14881 atgatgcaga cgctttttcg gaaccctttg gccgatccgt atattttggg cgtcagttcg
    14941 ggcgcaagtt tgggcacggc gctggtggtg atggcttccg gttttttacc gttttccttt
    15001 gtcggaaatg gttgggtact gataatttcg gctattgttg gcgctgcatt ggtgctgttg
    15061 attgttcttt ttgtttcctt tcgcgtcaac gatattgttt cgctgctcat tgtcggtatt
    15121 atgtttggaa ccattgcagc tgctttggtg tcgattattc agaatttcag caatcccgat
    15181 gccataaagc tgtttttgat gtggacattc ggaagtttaa gcgccgttac gtggaattat
    15241 ttgcaaattt tgttgcctgt tgttttggta ggcataggta ttgctttttt tctgcaaaaa
    15301 aggctcgacg gacttttatt gggcgaaaat tatgcacggg gtttgggtat tcccatttca
    15361 caaacccgat cgctgattat tctggctacc ggattactgg caggaggaat cactgctttt
    15421 actgggccca ttgcattcgt aggggtggca gttccgcata tagcacgagg aatttttcag
    15481 acttcatcac acaaaatttt attgccggca tgtatgctgg tcggtgcagc attgttgatg
    15541 atttgcgata tcattactca aattcccgat tatacgttgc cggtgaatac catcagtgcc
    15601 ttatttggtg cgccggtaat tatttggatc attttaagaa gaaaataagt caggaagaaa
    15661 ttataagtca ataatatgtc ggttttatcc acacaacatt tatcgattgg ttaccggaga
    15721 aaaggaacgg ctaacgtaat tcaacatgac ttgaatttaa agctggaatc agggaaactg
    15781 gtaggtttga ttggcccgaa cggaagtgga aaaactacgc ttttacgaac tattgcagga
    15841 ttgttgaaac ccatcgacgg aaatattttg atagatgaca aagagtttct tttgctttcg
    15901 caacgtgaaa aagcattgaa tgtttctttt gtacttaccg aaaaagtaga tattcccaat
    15961 gccgatgttt tcgatatggt atcctttggg cgttttccct ataataattg gcggggaaat
    16021 ctttccaagc aggacagaac gaaagttttt caggcgctgc aaatggtcag gatggaaaaa
    16081 atgatgcatc gatatatcaa tgaactttcc gacggagaac gtcaaagagt catgatagcc
    16141 aaagcgttgg ctcaggatac gcccattatt ttactcgatg aaccaactgc ccatctcgat
    16201 gttcccaatc gtgtgggaat tatgttgctg ctccataaat tggctcatac aactgggaaa
    16261 atgattctgc tttcgacaca cgaactggat ttggcgttac aggctgctga ccttatttgg
    16321 ctgatgagcg agaatggagg aattgaatgc ggagtccccg aagatttggt tttcagcggg
    16381 agtttcaatc gaacttttgc cagcgattat tattttttca atgaagccaa tggtaacttt
    16441 tccctgaatt atcctttgac aggaaaggtg agcgtatcgg gcaacaaaat gcgtatatat
    16501 tggactttgc gggctttggc acgtgcagga tatgaagcgg tggaaaatgc tccgttgaaa
    16561 atagaagtta ccgacagtgc atggatactg aatcaaaagg aaattttttc ggtggccgaa
    16621 atgttggatg aattgaataa tttgaatggc aacacttcat tataatttat tttgacttct
    16681 tagtcagttt aacgtaaata ttcactaccg atattcctat atatcgatta actgcgcctg
    16741 tttgtcccaa ctttacaagg tagttcttta ctataaagat aaccataatc atcaattcct
    16801 tcaactaaaa taattgattt ttaatttatt aagtgaattt ttcaccgaag tattatgaat
    16861 gaagttgtcg atattaaatt ctttttcttt agattgacta atatctatgc ttagacacat
    16921 atttttaaat ggggacccat aaatttggtt aattggattc ttgaaaaaac aggatgagaa
    16981 ataggttatt tgtcggtttt ttttctttat tttgtattga attacattac caaattaatt
    17041 tttaaaaggc tatgagcaaa aaattgacgc gttcgagcaa tcaaatgata gccggagttt
    17101 gtgccggaat agcagaatat tttggttgga atgtaacttt agtaagagtt atttatgttt
    17161 tgctttccat cttttctgca ggattccccg gtactatggt ttacattatt ctttggattg
    17221 tcatgcctcg ccccgagaaa ttcgattaaa taaccgaaag aacaaaagtc gttgtgaaaa
    17281 cgacttggaa acaaacctat aatttacact tttttaaacg ttatgaattt taagctcgaa
    17341 tcgccctatc agcctaccgg tgatcagccg gaagccattc agcaactggt agagggaatc
    17401 aatgccggta ttccgtatca aactttgttg ggtgttaccg gatcggggaa aacctttacc
    17461 atagccaatg ttattgagca ggttcagcgt ccgacgctgg tactgagtca caacaaaacg
    17521 cttgctgccc agttgtacag cgaattcaaa tcttttttcc cacacaatgc cgtagaatat
    17581 tttgtttcct attacgatta ttatcagcct gaagcgtatt tgccattgac cgatacgtat
    17641 atcgaaaaag atttgtccat caatcaggaa atcgaaaaac tgagactctc ggccacctct
    17701 tcgcttcttt cgggacgcag ggatgttgtg gtggtatcat cggtttcgtg tctgtatggc
    17761 attggtaatc ccgacgattt ttcgaaaaat gtaattgaaa ttcgaaaagg acagcatttg
    17821 gtgaggaatg cttttttaag aaatttggtc aacagccttt atacccgtaa cgaaattgaa
    17881 ctgaatcgtg gaaattttcg cgtaaagggc gacactgtgg atatttttct tgcctatacc
    17941 gattttattt tgcgtatcgt tttttgggga gacgaaattg atgagatact gacagttgat
    18001 ccagtcaatt acgacattat tgattctctg gatgcctata aaatatatcc ggccagcatt
    18061 tttgtaacca caaaggaacg aattgctgtt gccataaatg aaatcgaaaa agatttgcag
    18121 ttgcaggtgg attattttcg ctccattgga aaagatctcg aagccaaaag aatttatgaa
    18181 agagtacgtt acgatatcga aatgataaaa gaacttggtt attgctcggg aatcgaaaac
    18241 tattcgcgat atttcgacgg tcgtccgccg ggaagccgtc ctttctgtct gttggattat
    18301 tttccgaaag attttctgtt ggtcattgat gaaagtcatg ttaccattcc tcaaataaga
    18361 gccatgtacg ggggcgatgc ttcacgtaaa cagaatttgg tcgatttcgg attccgattg
    18421 ccggctgcca aagacaatcg tcccttgcgt tttgaagaat ttgaacagct tacacctcaa
    18481 actatttatg tgagtgcaac ccctgctgat tatgagttag taaaatcgga aggtattgtg
    18541 gttgatcagc ttatacgtcc taccggactg ctcgatcccg ttattgaggt tcgtccgagt
    18601 ttaaatcaga ttgacgattt gctggaagaa attgcccagc gaaccgaaaa caacgagcgg
    18661 gtgctggtaa ctacccttac caaaagaatg gctgaagaac tgaccaatta tctatcaaaa
    18721 atgggagtga aatgcaatta tattcattct gatgtcgaaa cgctggatcg ggttcaaatc
    18781 atggaagatt tgcgcagagg agtgtatgat gtgttggtcg gtgtcaacct gttgcgcgag
    18841 ggattggatt taccggaagt ttcactggtg gctattttgg atgccgataa ggaaggattt
    18901 ttgcgttctc atcgttcgtt gacgcaaacg gcagggcgag ctgcacgaaa tttgaatggg
    18961 aaagtcatca tgtatgccga tcagatcacc gaaagcatgg aaaagaccat aaatgaaacc
    19021 aatcgccgca gagagaagca acttcgatac aatgaagcgc atcatattac gccaactgct
    19081 attgtgaaag gcgaaagaaa tccgctgtcg aaagcagaac catacgcgta tgtggaaccg
    19141 gaaaagtcga ccgatattgc agccgatccg gtggtgcgat atatgtccgt gccggcactt
    19201 gaaaaagcca tagcaaaaac cagaaaagcg atgcaggaag ctgccaagaa actcgaattt
    19261 ctggaagcgg ctcaactcag ggacgaattg atgaaactgg aagaattgtt gaaaaagaaa
    19321 atgttcgagg cgaagaattg aaaagaaaat gttgctttaa aagtttcaaa aaatcaattt
    19381 cataagtcga aacaaaagga caattcattc aaaaaacacc aaacaagccc taaaaattgt
    19441 tgaattattg cattaaattt agttttctga tgatagaaaa attatctttg tatcgctaat
    19501 tcaccagaaa acctataata tgctggaaat cattccgaaa ggtattctaa ttggtttgtg
    19561 tatttctgtt ccggtaggtc ccattggtat gctggttatt cagcgtacct tgaatagagg
    19621 aagaaaatac ggagtggcca ccggtttggg tgcgagcatg tccgatttat tttataccat
    19681 catcacttta ttctttttga gttttgtgat cagttttatt gaggctcatc gttttgtcat
    19741 agaattgata ggaagtttgg ttgtgatttg ttttggatat ttcatatata aaaataatcc
    19801 gtcagtacaa cccaaaccaa acgagacagt tcagcacaat gtataccgcg actttatcag
    19861 ttctttcgta ttgactttct ctaatcccct cattttgttt attttgattg cattatttgc
    19921 cagaattgga tttattgact cagaaacatc tttttttcaa accattattg gtatcatatc
    19981 tattttaatt ggtgctacta tttggtggtt aactcttact tttattgtca gccatttcag
    20041 gtcaaaaatt ggaatgcacc agttgaaagc cataaatcga ataacaggtg gactgattgc
    20101 tttgattgga tgtattggac ttgtttccag tttgatataa aaattcaccc ttctttcttc
    20161 gcagaaaaaa gggtgtactt cactatgggt gaatcattta acccagaatc cgcaataggg
    20221 aaaaagtttt aattttgtag gaaagtctaa tgcgtagcaa tagaaaaaaa gac
//
LOCUS       DAOSCH010000026        19948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_1912_length_19948_cov_6.145076, whole genome shotgun sequence.
ACCESSION   DAOSCH010000026 DAOSCH010000000
VERSION     DAOSCH010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 19948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19948
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1912_length_19948_cov_6.145076"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="PLZ54_03445"
     CDS             <1..1416
                     /locus_tag="PLZ54_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ66022.1"
                     /translation="LYGSNAMGGVINIITSKLYKDTVQQNFHFGYGSYSTLESSASNH
                     FHKNRITGKFAISYNRTDGHRENMDFDQISGYAQLGYRLDRYWRVSTNANISHFNASN
                     PGTIDQPIVDNDSHVKRGWINASVENRYRYTSGALSFFYNWGNHIINDGYNMGGQPLD
                     YLFHSNDKISGITFYQSAILMPNNKITFGFDYQKFGGKAWNKYNDGNKKSIIDTTLYE
                     IAGYADMRQDFGLLATVDIGIRLDNHSKTGSQWIPQVGLSFHLPKNIEAKTIVGKGFR
                     NPTIRELFMFTPQNPNLLPEEVMNYEFSVSQNMVEKNLFYDVAVFYLEGKNMIQTVIS
                     NGRPINVNTGKIKNFGFESVITYQINSIWSANVNYSWLDMKYPVTGAPKQKVFAGFDY
                     SKKKLKFSSGIQYVHRLYSSLNPVKKENFTLLNARTTYQLGKFTQLFLTGENLLNCSY
                     EINAGYPMPKATFLIGCNVHF"
     gene            complement(1516..2322)
                     /gene="mazG"
                     /locus_tag="PLZ54_03450"
     CDS             complement(1516..2322)
                     /gene="mazG"
                     /locus_tag="PLZ54_03450"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOJ66023.1"
                     /translation="MEKTTHTMEEKLQEFKRLLEIMERLRAECPWDREQTFESMRVQT
                     IEETYELADAIMKDDKKAIMTEVGDLLLHVVFYAQMGSETGDFDIFDVCKNLNEKLIF
                     RHPHVFSNTKADNSQTVVQNWEDLKLKEKNGNRTVLAGVPETLPALIKAHRIQDKARN
                     VGFDWEKREQVWDKVQEEFQELKDEVEAMDKNKMEEEFGDLLFSLINAARLYDINPEN
                     ALERTNRKFIQRFNYLESQTIAQGKDLRQMSLDEMDVFWEEAKKKEKEEG"
     gene            complement(2478..4736)
                     /locus_tag="PLZ54_03455"
     CDS             complement(2478..4736)
                     /locus_tag="PLZ54_03455"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ66024.1"
                     /translation="MKKILILLSLVPTFIFAVDLEPKDSLKHVYLEEIQIISHKETLP
                     AQPAASYSVVSSNLINQAQVNSVRDLSILVPNFFIPDYGSSMSTSPYIRGVGARSTGQ
                     SIALYVDNVPYFEKSTFDFDFYDIQQAEVLRGSQSTLYGRNALGGVVNIYTLSPFEYQ
                     GTKLSVTGGNYGLLQAQASHYAKLSNKTGLSLSGYYHHRNGFFTNTFTGKDADWKNSA
                     GGRAKFDWHITPSLRAQYIINYDYVNQNAFPYGLYNATTKSTAEPNFNDKSSYLRNML
                     TNSVLIEYRNDKIQLTSTTSYQYFSDKMFMDQDFTPLSYFSLEQQQKQHAVNEEIVLK
                     SRTSGNYQWSFGATGFYQKLNTDAPVAFKQDALTNILQPVLDRAHTAGAPQMTITSTV
                     MDIPGTYDTKTAEGALYHQSTFNHLLTDGLSATIGVRADFEKVNLDYFTNTRLDLLMK
                     MGNRNIPTSLADTLRGAESVKFSEFLPKVSVKYAWNSQQYIYANVSKGYKAGGYNIQM
                     FADLIQNDLMNSGNSSASQPDVRKAILFQPEYSWNYEAGCQSTFLDNRLKMQLSLFYM
                     QITDMQLTRFVPSGNGRMITNAGKVDSKGMEFSLQANLGKGFAAGLNYGYAHAAFTNY
                     TDSVKSNGTVNEVDYKGKFVPYAPQNTLNIGGEYLRIFKNSFIDQFMVSMQYNAAGKI
                     YWDVDNSIYQDFYGLINAKVGVEKGNFRVELWAKNLFDTQYNAFYFESFENKFFQKGN
                     PAQFGLTLKTEF"
     gene            complement(5002..5358)
                     /locus_tag="PLZ54_03460"
     CDS             complement(5002..5358)
                     /locus_tag="PLZ54_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66025.1"
                     /translation="MMFDNILFASFVPQILMILGYIMCLSAATSSSHVSGISEITPIT
                     QHQVFFSTENKIETENTFQFEDYFFVSAIIPSKESIKPIYHFPTIIPLVKCNACISNG
                     FLAELFCRPPPTLSFC"
     gene            complement(5380..5452)
                     /locus_tag="PLZ54_03465"
     tRNA            complement(5380..5452)
                     /locus_tag="PLZ54_03465"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5417..5419),aa:Thr,seq:cgt)
     gene            complement(5540..6223)
                     /locus_tag="PLZ54_03470"
     CDS             complement(5540..6223)
                     /locus_tag="PLZ54_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOJ66026.1"
                     /translation="MNLIETLRIWDTNLFLALNGIHSPFFDGFMMAFSNKLVWIPFYI
                     SVLYVMIKQWKSEAFWIVLSFAICILLADQISSGIIKNAVQRLRPSREETISGLVHLV
                     DGYRGGKYGFVSSHAANSLGFAVLSAYFFRKKSYTLIILLWSLITGYSRIYLGVHYPL
                     DVMGGYLVGFFVAIFCYWVLKKLKPNIFYVSSRFRSKRFADSTLPLLVLTFTVVGIFG
                     WSLIRILAF"
     gene            complement(6263..7021)
                     /locus_tag="PLZ54_03475"
     CDS             complement(6263..7021)
                     /locus_tag="PLZ54_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ66027.1"
                     /translation="MKRLISNIILIITLLASLPLSAQSDAIKYANDLYAKGNFHAAAE
                     QYEKILKNEGVAPELYYNLGNAYYKMNEIGLAILNYERALKLSPRFEDARFNLQLAQQ
                     KVLDNVNYNQQFFIQRWITNLMHMFTSNQWFTAGYLFFLAALIFAFLFAFGSSVRQRK
                     NSFYIGIVLLLFAVTSIIFSGIRKNEETNSLQAIIMTGVVTVKSSPDDSGTDLFQLHE
                     GTKVTIKSTLGDWAEIKIGNGNVGWVESKSIEKI"
     gene            complement(7131..8954)
                     /locus_tag="PLZ54_03480"
     CDS             complement(7131..8954)
                     /locus_tag="PLZ54_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOJ66028.1"
                     /translation="MKRKIFLLFLIFAGFIGVKADEPVRFTATAPSTVILDKPFQLVY
                     TINAQGKDLRMPEIKDFDILAGPFESRSSSIQIVNGKRTSSLSISYTYTLMPQRTGTF
                     TIPSASIVVNNQKYTSNGLTIKVLPADEQSDESSSPADNGRKSSPKNISNENIFIRTI
                     PSKTSVYEQEALLITYKLYTLVDVAQCVNKKMPDFDGFLKQEIEQTQNKQFSYENYNG
                     RNYGTVVLYQTLLYPQRAGTIQIDNAVFESVIRVQNQTSIHSIFDDFFDSYTNVTKNL
                     VAPGLKITVKPLPEDKPTIFGGAVGNFTMSSSISAQEVKENEAVTLKINIAGSGNMKL
                     IKTPEVKFPSDFEIYDPKVTNNFKTSASGVSGTKTIEYLFIPRHSGNFEIPPVEFSYF
                     DLRDKTYKMLRTPSYSLKVLKGSGNQNATVVSNYNPKEDIRQINKDIRYIYTGNIRLI
                     KPSTPVFGTTLAWLLFIIPLVVVVGLFFFFRKKVKENADIALVRNKKANKMAQKRLKL
                     AQKLLKEGNKDAFYDEILKATWNYLSNKLSIPVASLTRETVSEELKLRGVTDELTKKL
                     MKILNDCEFARYAPKSGQQEMGNIYEETIQVISELEEAIKK"
     gene            complement(9048..>9242)
                     /locus_tag="PLZ54_03485"
     CDS             complement(9048..>9242)
                     /locus_tag="PLZ54_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66029.1"
                     /translation="QDNQQNKQNDNQKDQKNQQQQQQQQMTKENAQQILDALSQDEKE
                     TMNKAKKLPSKQEQKPDKDW"
     assembly_gap    9243..9342
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9343..9660)
                     /locus_tag="PLZ54_03490"
     CDS             complement(<9343..9660)
                     /locus_tag="PLZ54_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ66030.1"
                     /translation="MKSKFQNFSVKTNVHSEFKKGVFLFLIMSVLSVSVSAQKGNNEV
                     RAGNKKYNNKKYIEAEIDYRKGLEKNPKSFEGNYNLGNSLFRQKKYEEAYQQYEKAAS
                     YAPK"
     gene            complement(9650..10684)
                     /locus_tag="PLZ54_03495"
     CDS             complement(9650..10684)
                     /locus_tag="PLZ54_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ66031.1"
                     /translation="MFRFAHPEYFFLLIVVPVLIGTFLYTQALKKRRLKKFGDPELLA
                     ELMPNVSYRRPVVKFFLQLFAVILLIIVLAQPQFGSKVENVKRQGIDVMIALDVSNSM
                     MAQDIQPNRLEKAKQILSKLIDQMNDDRIGLIVFAGDAYTQLPITVDYVSAKMFLSAI
                     STNMVPRQGTAIGTAIDLAIKSFGQKSQSGKAIIVITDGENHEDDAISAAKLAAQNGI
                     NVHVIGVGSPEGAPIPIPGTMSFKKDKDGNVVVSKLNEEMCKNIAVAGNGIYVRADNT
                     NNAYRIISKQLDTLAKSTTESKVFSDYSEQYQSFALIALIVLIIDFFLFDRKNKKLSK
                     IKIFDIENEK"
     gene            complement(10741..11724)
                     /locus_tag="PLZ54_03500"
     CDS             complement(10741..11724)
                     /locus_tag="PLZ54_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOJ66032.1"
                     /translation="MTFHNPGYLFLLLLLLPIIYWYIKEMHKSDASLQISSLQDLTKF
                     PKSKRIKMRHLPFILRLFTLIFLIIAIARPQSSNSLRNETTEGIDIMIALDISGTMLA
                     EDLKPNRLEASKAVATEFILSRPNDNIGLVIFAAESFTQCPLTTDHDVLINLFKGVQY
                     GMIEDGTAIGLGLANAVNRIKDGKAKSKVIILLTDGSNNRGDIAPVSAAEIAKTFGIR
                     VYAIGVGSHGMVRVPVPTPLGMQYQLMESEFDEQTLKAIANITGGKYFRATDNLKLRS
                     IYQEIDQLEKTKISVREYSKKNEMFYLFALAAFLCLISEILLRNTVLRKIP"
     gene            complement(11777..12775)
                     /locus_tag="PLZ54_03505"
     CDS             complement(11777..12775)
                     /locus_tag="PLZ54_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HOJ66033.1"
                     /translation="MRKRNKHIKLLINLCISLLVSTTLSAQKFSVTVKVDSSVIWIGD
                     QTGFTLEVSQAIGQKVNFPLFSDTLIHGLEIVEPLKIDTVAGDNNSIRVLHKYIITAF
                     IDTLYYIPSYPFVEGTDTVWSNSVSLKVVQPFQIDTASNSITDIKPVFQPKYDWSGLF
                     KKILLTLLVLAVLAAAYYFIRKKIQKKPVILIHKEEPAIPPHEAALHALDKIKEEKLW
                     MKGKYKEYHTQLTDVIREYIERRFLINSMEMTSSEILDNLPKNIYKEQKTAFSALHQL
                     LQLADLVKFAKWVPSPEENISSLNNAYLFVEQTKEETPLTEEKQTEKPVDKKEGEE"
     gene            complement(12794..13672)
                     /locus_tag="PLZ54_03510"
     CDS             complement(12794..13672)
                     /locus_tag="PLZ54_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOJ66034.1"
                     /translation="METSEILKKVRKIEIKTRGLSQDIFAGAYHSAFKGRGMTFSEVR
                     EYQYGDDIRTIDWNVTARFGRPYIKIFEEERELTVVLLIDVSGSQEFGTVSRMKKDMM
                     TEIAATLAFSTIQNNDKIGVIFFSDKIEKFIPPKKGRKHVLYIIRELIDFRPESNKTN
                     LAGALRYFTNAIKKRSTAFVISDFMDSNFDNELTIANRKHDVVALQIYDERETEMPDI
                     GLVKLKDAETGQRIWIDTADKHLRTFYKHHWGERQLNLQRAFTKAKVDAVSMNTSDDY
                     VQALMRLFQMRSKIGR"
     gene            complement(13792..14787)
                     /locus_tag="PLZ54_03515"
     CDS             complement(13792..14787)
                     /locus_tag="PLZ54_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOJ66035.1"
                     /translation="MDQSVDIRELNERIERESSFVDALVMGMNKVIVGQKHLIESLLI
                     GLLADGHILLEGVPGLAKTLAIKTLSDLIKGNFSRIQFTPDLLPADVVGTMIYSQKKE
                     EFSIRKGPIFANLVLADEINRAPAKVQSALLEAMQERQVTIGEKSFKLEEPFLVMATQ
                     NPIEQEGTYPLPEAQVDRFMLKVIINYPKKDEEKLIIRENLQKNMPSVQPILSPEDIV
                     KARDIVRQIYIDEKIEHYIVDIVFATRFPEEYGLESLKNMISFGASPRASINLALAAR
                     AYAFIKRRGYVIPEDVRAVCYDVMRHRIGLSYEAEANNMTSEEMITEILNTVEVP"
     gene            complement(15020..15937)
                     /locus_tag="PLZ54_03520"
     CDS             complement(15020..15937)
                     /locus_tag="PLZ54_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HOJ66036.1"
                     /translation="MNIKFHFLLFTAIWLSECLPFPLNAAKPQKEPIKILAIGNSFSD
                     DAVEYLDELAIADSVPLIVGNLYIGGCSLERHWKNATELLPDYSYRKSNLGIFTNQDH
                     KTLLDGLLDENWDIVVVQQVSQLSGKYDTYFPYLPNLMRFVKTHAKNPKVKFALHQVW
                     AYAQNSTHEGFANYDRDQMKMYLAITETVKKVAATTGIKIIIPSGTAIQNCRTSEIGD
                     HLNRDGYHLDLHIGRYVAACTWFETFFHRSCVGNPFKPAGVTDRQAKIAQYAAHYAVQ
                     RPFEITLMTNFAWTFLPSSEKTISEFLVY"
     gene            16414..18162
                     /locus_tag="PLZ54_03525"
     CDS             16414..18162
                     /locus_tag="PLZ54_03525"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOJ66037.1"
                     /translation="MENDALLKEVMSKAQKWLDGNYDENTKNEIRRMMAAEDKTELIE
                     AFYKELEFGTGGLRGIMGVGTNRMNIYTVGSATQGLSNYLKAQFSDLPQISVVIGHDC
                     RNNSRHFAEISANIFSANGIKVYLFENLRPTPETSFAIRYLGCQSGIIITASHNPKEY
                     NGYKAYWNDGAQMVSPHDVNVIAEVNKITRVEDIKFEGNPELIEIIGEKIDKAFIDAI
                     KTLSLSPEAIRRHSNLKIVYTPLHGTGITLIPRALEEYGFKNIIHVPEQDVISGDFPT
                     VVSPNPEEPAAMEMAVNKAKEVDADIVMASDPDADRMGIAVKNDKGEWILVNGNQTAL
                     LFVYYLIRRWKELGKLTGNEYVVKTIVTTELIKNIADRNGVECFDCYTGFKWIASVIR
                     ENEGKKVYIGGGEESYGFLPEDFVRDKDAVSCCALMAEITAWAKDQGKSLFELLQDIY
                     LEYGYGKEKGISIVKKGKSGADEIQEMMRNFRNHPPKEIAGSKVSIIKDFQTLKMLNL
                     KTNEENSLNMPATSNVLQFFTEDGTKISVRPSGTEPKIKFYIEVRGEMKSRADFDKAE
                     TEALKKIEAVRASLGI"
     gene            18261..19205
                     /locus_tag="PLZ54_03530"
     CDS             18261..19205
                     /locus_tag="PLZ54_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567067.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOJ66038.1"
                     /translation="MGMDTGFQQRYLNYNQVLKSEFDERIQKISVHAGFTCPNRDGTK
                     GWGGCTYCNNQTFVPEYCKPVKSITQQLDEGVNFFRHKYKSQYYLAYFQSYTNTYDSF
                     ENLKSLYEEALHHPKIKGLVIGTRPDCVSEDVLDYFERISKNSYVLIEYGIESTSDAT
                     LEFIRRGHDFACAEFAVKETARRGIKTGAHLILGLPFESKEIILEHADKISRLPLTAL
                     KLHQLQIVKGTVMAVQYRQHPEWFQLFTVDEFVDLVVDFLERLSPNIAVERFVSQSPE
                     GLVIAPQWKIKNFEIASKIEKQLIKRNTFQGRCWKGND"
     gene            complement(19290..>19948)
                     /locus_tag="PLZ54_03535"
     CDS             complement(19290..>19948)
                     /locus_tag="PLZ54_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ66039.1"
                     /translation="SLNVSSASVSRNTSKKLFEASSYLFSYSFSEPEAYATYLATTLG
                     FVATHDKVVGLHVYGDLSGNSIELRFVAGAEVKYVKLCDLNFFGWEFVETKLDHLQEG
                     LPYEFTGFRVVRNEGILSGNGEIYFDNMLLYDEPIMAIPQVLKNKILIYPNPASNMVM
                     VKTSDQFISQMQLYSLQGKLLKETNKTNLDISNVAAGTYVLKIKTEKDIVSFPLIIVH
                     "
BASE COUNT         5886 a         3751 c         3884 g         6327 t
ORIGIN      
        1 ctttacggat ccaatgccat gggtggcgtt atcaatatta ttacttcaaa actttacaag
       61 gataccgttc aacaaaattt ccacttcggt tacggctctt acagcacgct ggaatcttcg
      121 gcaagcaatc acttccataa aaatcggata accggtaaat ttgccatttc atacaatcgt
      181 actgatggac accgagaaaa tatggatttc gaccaaataa gcgggtatgc acaactcggt
      241 tatcggctgg acagatactg gcgtgtttcc actaacgcaa atatttctca tttcaatgct
      301 tccaatcccg gcactatcga tcagccgatt gttgacaacg attcgcacgt taaaagggga
      361 tggataaatg cttcggtaga aaacaggtac agatacactt caggagcttt gagttttttt
      421 tacaattggg gaaatcacat tataaatgat ggatacaaca tgggaggaca gccactcgat
      481 tatcttttcc actccaacga taaaatatcc ggtataacgt tttatcaatc agcaatttta
      541 atgcccaaca acaaaattac ttttgggttc gactatcaaa aatttggagg caaagcatgg
      601 aataaataca acgatggaaa taaaaaatcg attatcgata ctaccttgta cgaaatagcg
      661 ggttatgctg atatgcgtca agacttcggc ttgctggcca cagtggatat aggcattcgc
      721 ttagataatc attccaaaac cggttctcaa tggattcctc aagtgggctt atcctttcat
      781 ctccccaaaa atattgaagc aaagacaata gttggcaaag gattcagaaa tccaaccatc
      841 agggaacttt ttatgtttac tcctcaaaac cccaatttat tgcccgaaga agtaatgaat
      901 tatgaatttt cagtttctca aaatatggtt gaaaaaaatt tgttctatga tgtggctgta
      961 ttttatcttg aaggaaaaaa tatgattcag acggtaattt caaatggaag accaataaac
     1021 gtaaatacgg gaaaaattaa aaactttggc ttcgaaagcg tcataactta tcaaataaat
     1081 tcaatttggt cggcaaatgt caactacagt tggctcgata tgaaataccc agtaaccgga
     1141 gcacccaaac aaaaagtttt tgcaggattc gactactcga aaaaaaagct aaaattttct
     1201 tccggaattc aatatgttca tagactttat tcttctctca atcctgtgaa aaaggaaaat
     1261 tttaccttgt tgaatgccag aacgacgtac caattgggta aattcaccca actattttta
     1321 acgggtgaaa atcttttaaa ctgctcttat gaaataaatg ccggttatcc catgccaaaa
     1381 gccacatttt taataggctg caatgtccat ttttaaaaaa cttcgatttc tttcaattat
     1441 tcaaatgata aataattgta ggctaatact ttattaaggc tttgctgcca gaaaagaaaa
     1501 aaataaaatt gatttttaac cttcttcttt ctctttcttt ttggcttcct cccagaaaac
     1561 atccatttcg tccaacgaca tttgtcgcag gtcttttcct tgcgcaatgg tttgagattc
     1621 gaggtaattg aatcgttgaa taaatttacg gttggtgcgt tcgagtgcat tttcgggatt
     1681 aatgtcataa aggcgggctg cattgatcag gctgaaaagc aagtcgccaa attcttcttc
     1741 catcttattt ttatccatcg cttctacttc gtcttttagt tcctgaaatt cttcctgaac
     1801 cttatcccat acttgttcgc gtttttccca gtcaaatcca acatttcgcg ctttgtcctg
     1861 aatccgatga gcttttatca atgccggtaa agtttcggga acgccggcaa gaacagttcg
     1921 gttgccgttt ttctccttta atttcaaatc ttcccaattt tgaacgaccg tttggctgtt
     1981 gtcggctttg gtattggaaa atacgtgagg atggcgaaaa atcagttttt cgttcagatt
     2041 cttacaaaca tcaaaaatgt cgaagtcgcc ggtttcgctt cccatttgtg cataaaaaac
     2101 cacatgaagc aacaagtcgc ctacttccgt catgatggct tttttgtcgt ctttcattat
     2161 tgcatcggcc aattcataag tttcttcaat agtttgaacg cgcatgctct cgaaagtttg
     2221 ttctctatcc caagggcatt cggctctcaa ccgctccatt atttccagca aacgtttaaa
     2281 ttcctgaagt ttttcctcca ttgtatgtgt tgttttttcc ataatttcag ggtgaagtat
     2341 gtataaattt tatgcgaaat tacttatttt ttttcgatga agagattttt ccgaaaatat
     2401 aaaatccatt acgagaataa gtttttctcg taatggatca atgatactga agcaaaagtt
     2461 attttttaaa agttttttca gaattcggtt tttaaagtca aaccgaattg tgcaggattt
     2521 cctttttgaa aaaacttgtt ctcaaaagat tcaaaataaa aagcattata ctgggtatcg
     2581 aacagatttt ttgcccaaag ttcgaccctg aaatttcctt tctccacacc aacttttgca
     2641 ttgattaaac cataaaagtc ctgataaatg gaattgtcca catcccaata aattttcccg
     2701 gctgcgttgt attgcattga aaccatgaat tgatcaataa acgagttttt gaatatgcga
     2761 agatattcgc ctccgatatt caaggtgttt tgaggagcat aaggaacaaa ttttcctttg
     2821 taatccactt cgtttacagt tccgttactt tttactgaat cggtataatt ggtaaaggct
     2881 gcatgtgcat aaccatagtt aagaccggcg gcaaaacctt ttcccaaatt tgcctgcaat
     2941 gagaactcca ttcctttgct gtccaccttg cctgcgttgg ttatcattcg tccgttgccg
     3001 ctgggaacaa atcgggttaa ttgcatatcg gtgatttgca tgtaaaacaa cgacaattgc
     3061 attttcagtc tgttgtccaa aaatgtcgat tggcagcctg cttcgtaatt ccaactgtat
     3121 tccggttgaa acaaaatggc tttgcgaaca tcgggttgag aagcagacga atttcccgaa
     3181 ttcattaaat cattctgtat caagtcggca aacatctgta tgttgtaacc tcctgctttg
     3241 taacctttcg aaacgttggc ataaatgtac tgctggctgt tccatgcgta tttcacactg
     3301 acttttggaa gaaattcgga aaatttcact gattctgctc ctcgaagtgt atcggccaaa
     3361 gaagttggaa tgttcctatt tcccattttc attaataaat ccagtctcgt attggtaaaa
     3421 tagtctaaat ttactttttc aaaatcggca cgaacaccta ttgtcgccga aagtccgtcg
     3481 gttaaaagat gattgaaggt cgattggtga tacaaagctc cttcggcggt ttttgtgtca
     3541 taagttccgg gaatgtccat tacagtactg gtaatggtca tttgtggcgc tccggcagta
     3601 tgtgccctgt ccagtaccgg ttgcagaata ttggtcaatg catcttgttt gaaagctacg
     3661 ggcgcatcgg tatttaattt ttgataaaag cctgtagctc cgaaactcca ctgataattg
     3721 ccggaggttc tcgatttcaa tacaatttct tcgtttactg catgttgttt ttgctgttgt
     3781 tcgagagaaa agtaggaaag aggcgtaaaa tcctgatcca taaacatttt atccgaaaaa
     3841 tactggtaag aagtagtaga agtcagttgt attttgtcgt tccgatattc aatcagtaca
     3901 ctattggtaa gcatgttgcg taaataggaa cttttatcgt taaaattcgg ttctgcagtc
     3961 gacttggtag ttgcattata cagtccgtaa gggaatgcat tttgattcac ataatcataa
     4021 ttgataatgt attgagcacg cagcgaaggc gtaatatgcc agtcgaattt agctctacct
     4081 ccggcagaat ttttccaatc ggcatctttt ccggtaaaag tattggtaaa gaatccgttg
     4141 cgatgatgat aataaccgct tagcgacaat cctgttttgt tgcttagttt ggcataatgc
     4201 gaagcttgcg cttgcaataa accgtaatta ccacctgtaa ccgataattt tgttccctga
     4261 tattcaaagg gagaaagtgt ataaatattt accacgcccc ccaaagcatt gcgtccgtac
     4321 aaagtacttt gtgaaccacg caaaacttca gcctgctgta tgtcgtagaa atcgaaatcg
     4381 aacgttgatt tttcaaaata tggaacattg tccacataca aagcaattga ttgtccggtg
     4441 ctgcgcgctc caacaccacg aatgtaggga gaggttgaca tggaagaacc gtaatcggga
     4501 ataaaaaaat tgggaacgag tatgctcaag tcgcgaacgg aattgacttg tgcctgattg
     4561 ataagattcg atgaaacaac cgaataagaa gcagccggtt gtgcaggtaa ggtttctttg
     4621 tgtgaaataa tctgaatttc ttcgagataa acatgtttca atgaatcttt cggttccaag
     4681 tcaaccgcaa aaataaaggt agggactaaa gaaagtaata tcaatatttt tttcattttt
     4741 tttgatttta atatggaaaa acaaatacaa tagtgaatat ttattcagca ttttttgtat
     4801 gcaatatgct gcaagattgt taaaaaaacc gattttcgtt tattgagttt tttcagattg
     4861 aattaaagct caataattca ataaaaacgt ccaatttttt cgattttctt tcgtctttta
     4921 tactgcaaca aacagccatt aatgaagggt tttaggaaaa aattataagt gtaattgccg
     4981 aaacacagga atatttttga tttaacaaaa ggagagagta ggaggtggac gacagaaaag
     5041 ttctgccaag aaaccattcg aaatgcaggc attacatttt acaagaggga ttattgtggg
     5101 aaagtgataa attggcttta ttgattcttt ggatggaatg atggctgaaa caaaaaaata
     5161 atcttcaaat tgaaaagtat tttcggtttc aatcttgttt tcagtcgaaa aaaatacttg
     5221 atgttgtgtt atgggagtaa tttcactaat gcctgaaaca tgagaagatg aagtagctgc
     5281 cgacaaacac atgatataac ccaatatcat cagtatttgt ggaacaaaag aagcaaaaag
     5341 aatattgtca aacatcatct tttggaaatc gttataaaga gcctttttgg ggactcgaac
     5401 cccagaccta ttcattacga atgaatcgct ctaccagctg agctaaaaag gcactatttt
     5461 attaacggct gcaaaattaa tcaaaaaaat cgagatattt gcaggtgttg aagtcaaaat
     5521 ttttagaaca agcaattctt taaaaagcca atatccgaat cagactccaa ccaaaaattc
     5581 ccacaaccgt gaaggtaagc accaatagcg gtaaggttga atcagcaaaa cgcttgcttc
     5641 gaaaacggga agaaacgtaa aaaatattcg gtttcagttt ttttagtacc caatagcaaa
     5701 aaatagctac aaaaaatccg accaaatacc ctcccatgac atcaagagga taatgtacac
     5761 ccaaatatat tcgtgaatag cccgtaatca acgaccagag taaaataatc agcgtgtagc
     5821 tttttttacg gaaaaagtaa gccgaaagaa cggcaaaacc aagtgaattg gcagcatgcg
     5881 aagaaacgaa tccatatttt ccaccacgat aaccatccac caaatgaacc agcccactaa
     5941 tagtttcttc gcgcgaagga cgcaggcgtt gaacggcatt tttaataatt cccgatgaaa
     6001 tttgatcggc gagtaaaata caaatggcaa aagaaagaac aatccagaaa gcttccgatt
     6061 tccactgttt aatcatcacg tacaaaacgg aaatataaaa cggaatccat accagtttgt
     6121 tgctgaaagc catcatgaac ccgtcaaaaa atgggctgtg tatgccattc aaggccagga
     6181 acaagttggt atcccaaatt cttaatgttt ctatcaggtt cattttccga agtatttgca
     6241 accgtgaaaa ccagtaacct ttttatattt tttctatgct tttgctttct acccagccca
     6301 catttccatt accgattttg atttctgccc aatcacccaa tgtacttttt attgtaacct
     6361 ttgttccttc atgcaattga aacaaatctg ttccactatc gtcgggtgag cttttcaccg
     6421 taaccactcc cgtcataata atagcttgaa gagaattggt ttcttcgttt ttgcgaatgc
     6481 cggaaaaaat aatactggta acagcaaaga gaagcagaac aattccgata tagaaagaat
     6541 tttttcgttg tcgtaccgat gagccaaatg caaaaagaaa tgcaaaaatc aaggcagcca
     6601 agaagaacag gtaacccgcc gtaaaccatt ggttggaagt gaacatgtgc atcaaattgg
     6661 taatccagcg ttgaataaaa aattgctgat tgtaattgac attgtccaat actttttgtt
     6721 gtgccagttg caaattgaat cgggcatcct caaagcgtgg tgaaagtttc aaagcccttt
     6781 cataattcag gatggccaat ccgatttcat tcattttata ataggcattt cccaaattat
     6841 agtagagctc gggcgctacg ccttcgtttt tcagtatttt ttcgtactgt tctgcagcag
     6901 catgaaaatt tcctttggcg tagaggtcgt tggcatattt aatggcatcc gattgcgctg
     6961 agaggggcag cgaagccaag agagttatta taagtatgat attggatatg agacgtttca
     7021 tagtttattt ttaaattttt tattcggttt gttacagcac agccgatatt ttatttgtta
     7081 agataaaatg gtgtttttaa acaagcttat cttcgctatc tgcgaattgt ttactttttt
     7141 atggcttctt ccaattcgct gattacttga atagtttctt cgtaaatatt acccatttcc
     7201 tgttgtccgg attttggtgc atatcgtgca aactcgcaat cgttcaaaat tttcatcaac
     7261 ttctttgtca attcatcggt tactcctctt agtttcaatt cctccgaaac cgtttctctg
     7321 gtaagtgagg caacaggtat ggagagtttg ttgctcaaat aattccaagt ggctttcaaa
     7381 atttcatcat aaaaagcatc cttgttgcct tctttgagta atttttgagc cagtttcaac
     7441 cttttctgag ccattttgtt ggctttttta tttctcacca aagcaatatc tgcgttttcc
     7501 ttgacttttt tgcggaagaa gaagaataaa ccaacaacca caaccaatgg aataatgaac
     7561 agcagccatg ccagggtagt gccgaaaaca ggagttgatg gttttatcaa acggatgttg
     7621 cctgtgtaaa tgtagcgaat gtctttgttg atttgccgga tatcttcttt cggattatag
     7681 ttgcttacaa cagtggcgtt ttgattgccc gaacctttca ataccttcag gctgtaactc
     7741 ggagtgcgca acattttata agttttgtca cggagatcga aataggaaaa ttctaccggt
     7801 ggaatttcaa aattccccga atgacgcgga ataaagaggt attcgatagt ttttgttcca
     7861 ctgacgcccg aagccgatgt tttaaaatta ttggtaactt ttgggtcgta aatttcaaaa
     7921 tcacttggaa atttaacttc cggtgttttt atcagtttca tatttccact gccggcgatg
     7981 ttaattttaa gtgttacggc ttcattttcc ttcacctcct gagccgatat ggaagaactc
     8041 atggtaaaat ttcccactgc tcctccaaaa atcgtaggtt tatcttcagg aagcggtttg
     8101 acggtaattt tcaatcccgg tgctacgaga tttttggtta cattggtata ggaatcaaaa
     8161 aaatcgtcga aaatactgtg aatggaagtt tgattttgaa cgcgaatcac cgattcaaaa
     8221 acagcattgt cgatttgaat ggtacctgcc cgttgcgggt agaggagtgt ttgataaagc
     8281 actaccgttc catagttgcg tccgttgtag ttttcgtaag aaaattgctt gttttgtgtt
     8341 tgttcgattt cctgttttag aaatccgtca aaatcgggca tttttttgtt tacgcactgc
     8401 gccacatcta ccagcgtata aagtttgtag gtaattaaaa gtgcttcctg ttcgtaaaca
     8461 cttgttttgg aagggatagt gcggataaaa atattttcat tggaaatatt tttcggggac
     8521 gatttccttc cgttatcggc aggagaagag gattcatctg attgttcatc ggctggtaaa
     8581 accttgattg tcaatccatt ggaagtatat ttttgattgt ttaccacaat gctggccgaa
     8641 ggaatggtaa aagttccagt ccgctgaggc attagggtat aagtgtagga aatcgacagg
     8701 gaagaagttc gttttccgtt gacaatctga atgctggaac tgcgcgattc aaaaggacct
     8761 gccaaaatat cgaaatcctt gatttcgggc atgcgcaaat cttttccttg tgcattgata
     8821 gtgtaaacca actgaaaagg tttgtccaaa attacggttg aaggagccgt agccgtaaaa
     8881 cggactggtt catcggcttt gacgccaatg aaaccggcaa aaattaaaaa gagaagaaat
     8941 attttgcgct tcatttttac aattccttaa atttttcttt gtctgttcgg attcttccaa
     9001 tttcgaagat tttttattac aaaaatacag ataaaaatcc aaaaaattta ccagtctttg
     9061 tcaggttttt gttcctgttt tgatggtaac tttttggctt tgttcatagt ttctttttcg
     9121 tcttgtgaca gggcatccaa aatttgctgc gcattttctt tagtcatctg ttgctgttgt
     9181 tgctgctgct gatttttttg gtctttttgg ttgtcgttct gtttattttg ctgattgtcc
     9241 tgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttttggtg catacgaagc
     9361 agccttttcg tattgctgat aagcttcttc atattttttt tgccgaaaaa gcgaatttcc
     9421 caagttgtag tttccttcaa acgatttggg atttttttca agtccttttc ggtaatcgat
     9481 ttcggcctcg atgtattttt tattgttgta ttttttgttt cctgctctca cctcgttgtt
     9541 ccccttttga gctgaaacag aaaccgaaag aacactcatt atcaaaaaga gaaatactcc
     9601 ctttttaaat tccgaatgta catttgtttt gacagaaaaa ttctgaaatt tacttttcat
     9661 tttcaatatc aaaaattttg attttgctca attttttatt tttcctgtcg aagaggaaaa
     9721 agtctatgat taatactatc agtgctatca aagcaaatga ctgatactgt tcgctgtagt
     9781 cggaaaaaac ttttgattcc gtagttgatt tggctagcgt gtcgagttgt ttggaaataa
     9841 tccgatacgc attgtttgta ttatcggctc tgacatatat cccgttgccg gctacagcaa
     9901 tatttttaca catttcttca ttcagtttgg agacgaccac atttccatct ttgtcttttt
     9961 tgaaactcat ggttccggga attggaattg gcgcaccttc agggctgcca acgccaatta
    10021 cgtgtacatt gataccgttt tgggcagcca gttttgcagc gctgatggcg tcgtcttcgt
    10081 gattttcacc atccgtaata acaataatgg cttttccgct ttgcgatttc tgtccaaaag
    10141 atttaatggc caagtcaata gccgtgccta tggctgttcc ctgtcttggc accatgttgg
    10201 tcgatatagc ggaaagaaac attttggccg atacgtaatc aaccgttatg ggcaattggg
    10261 tataagcgtc gccggcaaaa acaatcagtc ctatgcggtc atcattcatt tggtcaatca
    10321 gtttcgacaa tatttgtttg gctttttcga gtcggttggg ttgaatgtcc tgcgccatca
    10381 tggagtttga aacatccaat gcaatcatga cgtcgatgcc ctgacgttta acattttcca
    10441 cttttgaccc gaattgcggt tgtgccaaaa cgataatcaa caggattacg gcaaaaagct
    10501 ggagaaaaaa tttgacaaca ggacgccgat aagacacatt tggcattaat tcagccaaca
    10561 attccgggtc gccgaatttt ttcaaacgtc gtttctttaa agcctgcgtg tagagaaaag
    10621 tccctatcaa cacaggaaca acaatcagca gaaaaaaata ttccggatgt gcaaatcgaa
    10681 acataaaata tctgattaat ttttaatcta tttctttcat ttttaatgca aaaaattttg
    10741 tcagggaatt tttcgcaata ccgtatttct tagcagtatt tcagaaatca gacacagaaa
    10801 ggcagccagc gcaaataggt aaaacatttc gtttttcttg ctgtattcgc gaacactgat
    10861 ttttgttttt tccaattgat ctatttcctg gtatatactt ctaagtttca ggttgtcggt
    10921 ggcacgaaaa tatttacctc cggtaatgtt ggcaatagct tttaaagttt gttcgtcaaa
    10981 ttccgattcc ataagctgat attgcatgcc gaggggagta ggaaccggaa ctcttaccat
    11041 tccgtgagaa ccaacaccga tggcataaac cctgataccg aatgtttttg caatttcggc
    11101 agccgaaacc ggagcaatgt cgcctcgatt gtttgagccg tccgtcaaca aaataatgac
    11161 tttcgatttg gcctttccat cttttatccg attcacggca ttggccaacc caagtccgat
    11221 ggctgttcca tcttctatca ttccgtactg aacaccttta aaaaggttga taagtacgtc
    11281 gtgatcggta gtgagcggac attgggtaaa actttcggct gcaaaaatca ccaatccgat
    11341 gttgtcgttc gggcgggaaa gaataaattc cgtagctacg gcttttgatg cttccaatcg
    11401 attgggtttc aaatcttctg ccagcatggt tcccgaaata tcgagggcta tcattatgtc
    11461 gatgccttct gttgtttcgt ttcgtaaact attggaggat tgaggtcggg caatggcaat
    11521 aatcaggaat atgagggtaa ataaacgcaa aataaaaggt aaatgcctca tttttatcct
    11581 tttacttttc ggaaattttg tcaaatcttg caatgaagat atttgcagac tcgcatccga
    11641 cttgtgcatt tccttaatgt accaataaat aatcggtagc aacagcaaca agagaaataa
    11701 atatcccgga ttatgaaaag tcatttttta ttgtaagcgt tttttatctg tttaaccaaa
    11761 tttttttgct ttatctttac tcttcgcctt cttttttatc cacaggcttt tcggtttgct
    11821 tttcttctgt gagtggcgtt tcttctttgg tttgttccac aaacaaatag gcattgttca
    11881 aactcgaaat gttttcttcg ggcgatggaa cccacttggc aaatttcacc aaatcggcaa
    11941 gctgtaaaag ctgatgtaaa gctgagaatg ccgttttttg ttctttataa atatttttcg
    12001 gcaaattatc caaaatttcg ctggaagtca tttccatgct gttgatcagg aagcgtcttt
    12061 cgatgtattc ccgaatgaca tccgtcagct gggtatggta ttctttgtat tttcccttca
    12121 tccaaagctt ttcttccttg attttgtcga gagcatggag agctgcttcg tgtggcggaa
    12181 tggccggttc ttccttgtga atgagtatga caggtttttt ctggattttt tttctgatga
    12241 aataataagc cgctgccagc acagcaagca ccagcaatgt caacagtatt tttttgaata
    12301 atcccgacca gtcatatttt ggctgaaaaa cgggcttaat gtctgttatg gaattggaag
    12361 ccgtatcgat ttgaaatggc tgcactacct ttaacgacac agaattggac caaaccgtat
    12421 ctgtaccttc aacaaaagga tacgaaggaa tataatagag ggtatctata aaagccgtaa
    12481 taatgtattt gtgcaaaacg cgaattgaat tgttgtctcc tgcaaccgta tctattttta
    12541 gcggttcaac aatttccaac ccgtgaataa gggtatccga aaagagcgga aagttaactt
    12601 tttgccctat agcttgagat acttccaacg taaaacctgt ttgatcacca atccaaatga
    12661 cggaagaatc gactttaaca gtaactgaaa atttttgagc cgacagggta gtggaaacca
    12721 ataaactgat acacaagttt atcagcagct ttatatgttt attccttttt cgcatttttt
    12781 aaaaaattcc gtattaacgt ccaattttac ttctcatctg gaacagcctc atgagcgcct
    12841 gtacataatc gtcggaagta ttcatggaga cggcatctac ctttgctttg gtaaatgcac
    12901 gctgcaagtt cagttgtctt tctccccaat ggtgtttgta aaatgttcgt aaatgcttgt
    12961 cggccgtatc aatccagata cgttgaccgg tttctgcatc ctttagtttt acgagtccga
    13021 tgtccggcat ttccgtttct ctttcgtcgt aaatctgcag tgcaactaca tcatgtttac
    13081 ggttggctat ggtcagttca ttgtcaaaat tgctgtccat gaaatcggaa atgacaaaag
    13141 cggtcgagcg tttttttatg gcattggtaa aataccgcaa agctcctgcg agatttgttt
    13201 tgttgctttc ggggcggaaa tcgatcagtt ctctgatgat gtaaagtacg tgttttcgtc
    13261 cttttttcgg aggaatgaat ttttctattt tgtcggagaa aaaaatcacg ccaattttat
    13321 cgttgttctg aattgttgaa aaagccaatg tagctgcaat ttctgtcatc atgtcctttt
    13381 tcatgcgcga aactgttcca aattcctgac tgccggaaac atcaatgagc aaaacaactg
    13441 tcagttcgcg ttcctcttca aaaattttaa tgtaggggcg tccaaaacgg gcagtaacat
    13501 tccagtcgat ggtacgaata tcgtcgccat attgatattc acgcacttcg gaaaaggtca
    13561 ttcctcgacc tttaaaagcg gaatgataag cgcccgcaaa aatgtcttga gacaatccgc
    13621 gggtttttat ttcaattttt cgaactttct ttaaaatttc actcgtttcc ataaattagc
    13681 gtgtagatgg aaaattttct ccactttttt ccgacagttt tagctgcata tttttctctt
    13741 ttctgtctcc ggtggaacat ttttaaacac tcttaatgac aaagccgttt tttatggaac
    13801 ttcaaccgta ttcaaaattt cggtaatcat ttcttcggaa gtcatattgt tggcttcggc
    13861 ttcataactt aaaccgatgc ggtggcgcat tacgtcgtag caaacggcac gaacatcttc
    13921 gggaatgacg tatccgcgtc gtttgataaa ggcataagca cgtgctgcca acgccaaatt
    13981 gatggaagct cttggtgatg caccgaacga aatcatattt ttgagcgatt ccaatccgta
    14041 ttcttccggg aatcgggtag caaacacaat atccacaata taatgttcta ttttctcatc
    14101 gatatagatt tgtcgcacaa tatcgcgcgc ttttacaatg tcttcggggc tcagtatggg
    14161 ttgaacggaa ggcatgtttt tttgcaggtt ttcgcgaata atcagttttt cttcatcttt
    14221 tttcggataa ttgatgatga ctttcagcat gaaacgatcg acttgcgctt cgggaagcgg
    14281 atatgttcct tcctgttcga tgggattttg tgtggccatc accaaaaaag gttcttccaa
    14341 tttgaaagat ttttcgccaa tcgttacctg ccgttcctgc atggcttcga gcaaggcact
    14401 ttgcactttg gccggagctc tgttgatttc gtccgccaaa acgaggttgg caaaaatagg
    14461 gcctttacga atgctgaatt cttctttttt ctgactgtaa atcattgttc cgacaacgtc
    14521 ggcaggcagc aggtcgggtg taaactgaat tcggctgaaa tttcccttaa tcaagtccga
    14581 aagtgttttg atggccagcg ttttggcaag gccgggtacc ccttccagta aaatatgccc
    14641 gtcagccagt aaaccgataa gcaatgattc gatcaaatgt ttttgtccta caattacctt
    14701 gttcattccc ataacgagag catccacaaa tgagctttcc cgttcaatac gttcgttaag
    14761 ttcccttata tcaaccgatt gatccatatt gttttttctt tgtttgttta ttgaatttgt
    14821 tgagcaaata tacagctatt tgaaacgtta aaaatctgat ttaataactt ttaacctgaa
    14881 tttagatgat taagtaatat ccttcttagc gctattttgc agtgaaaaat gctttattct
    14941 tctcatagtt ggcttattga tatttttcac ttttcaactg tacagatagt ggaaataata
    15001 aattgaaatt tttcttaact taataaacca aaaattccga aattgttttc tctgaagatg
    15061 ggagaaaagt ccatgcaaaa ttggtcatca atgtaatttc aaagggacgc tgaacggcat
    15121 agtgagctgc atactgagct atttttgcct gtcggtcggt tacgccggca ggcttgaaag
    15181 ggttaccgac acagcttcga tgaaaaaagg tttcgaacca tgtgcaagct gcaacatacc
    15241 ggccgatatg caaatcgagg tgataaccgt cgcgattgag atggtcgcca atttcgctgg
    15301 ttctgcaatt ctgaatggct gttccggaag gaataattat ttttatcccc gtagtggctg
    15361 caaccttttt cactgtttcg gtgatggcca gatacatttt catttgatcc ctgtcgtaat
    15421 tggcaaaacc ttcatgggta gaattttgtg cgtatgccca aacctgatgc aatgcaaatt
    15481 ttacttttgg attcttggcg tgtgttttga cgaatctcat caagttaggc agataaggga
    15541 aataagtatc gtattttccc gaaagttggc taacctgctg cactaccaca atatcccaat
    15601 tttcgtcgag cagtccatcc aacagcgttt tgtgatcctg attggtgaaa atgcccaaat
    15661 tgcttttgcg gtaagaataa tcgggaagga gttccgtagc atttttccag tgcctttcca
    15721 gcgaacaacc accaatgtac aaattgccaa caatcagcgg aacgctgtcg gctatcgcca
    15781 gctcatccaa atattctacg gcatcatcgg aaaaactgtt gccgatggcc agaattttaa
    15841 tgggttcctt ttgcggttta gcggcattca atgggaaagg caggcattcg ctcagccaaa
    15901 tagcggtaaa caataagaag tgaaatttta tattcatggt ggtttggtgt ttttcgttga
    15961 tagtgaaaac tttctttact gcaaaaatag cattttcatg caaagccttt gatggaaata
    16021 gtcttgtttt aatctaattt aatggccaaa gcggattttt cactttttta tagagaaaag
    16081 atggtcaaat ttgaacttaa catcgaattt atcgagaaaa atttgatttt aattacccaa
    16141 tacttaaaaa tgagttcatg aaattattaa tgcaatttcg acttgatagt tgaatctatt
    16201 agttctaaaa aacagtctat tttttgacct aagacctaaa tttattattt ttgtcggaga
    16261 aaagccgaat ttgtttgata ttttaatctg caggtaattt cctgatttaa aaatttcatt
    16321 tgaaatttga tcgaaacaga aacaaaaaac aagtggggca gaagcgtttt tgaattttag
    16381 aaattaaaaa gttaacatca aaaatataaa aatatggaaa atgatgcatt attaaaggaa
    16441 gtgatgtcga aagcacaaaa atggctggat ggaaattatg atgaaaatac caaaaatgaa
    16501 atccgcagaa tgatggctgc cgaagacaaa acagagttaa tagaagcttt ctataaagaa
    16561 ttggaattcg gtaccggtgg tttgcgcggc attatgggcg tggggaccaa tcggatgaat
    16621 atttataccg taggatcagc cacgcaaggc ttaagtaatt atttaaaagc tcaattctcc
    16681 gatttacctc agatcagtgt tgtaattgga cacgattgcc gcaacaatag ccgtcatttt
    16741 gccgaaattt cggcgaatat tttttcggca aacggcatta aggtttattt gtttgaaaac
    16801 ttaaggccta cgcccgaaac ttcttttgcc attcgttatt tgggttgtca gagtggaatt
    16861 attattacag cttcgcacaa tccgaaagaa tataatggtt acaaggccta ttggaacgat
    16921 ggcgcacaga tggtttcacc tcatgatgtg aacgtaattg ccgaagtcaa taaaatcact
    16981 cgggtggaag acataaaatt tgaaggcaat cccgaactga ttgaaatcat cggagaaaaa
    17041 atcgacaaag cttttatcga cgcaattaaa actctttccc tgtcgccaga ggctattcgt
    17101 cggcatagca atttgaaaat cgtttatacg ccacttcacg gaacgggaat tacccttata
    17161 ccgagagcat tggaagaata cggttttaaa aatattattc atgtgccgga acaggatgta
    17221 attagtggtg attttcctac tgttgtttct ccaaatcccg aagaaccggc tgcgatggaa
    17281 atggctgtca acaaagccaa agaagtagat gccgatattg tgatggcttc cgatccggat
    17341 gccgacagaa tgggaattgc cgtaaaaaac gacaaaggcg aatggatatt ggtcaatggc
    17401 aatcagacag ctcttttgtt tgtgtattac ctgattcggc gctggaaaga attgggaaaa
    17461 ctcacaggca acgaatatgt cgtaaaaacg attgtaacga cagaacttat caaaaatatt
    17521 gccgatcgga atggagtaga atgtttcgac tgttatacgg gttttaaatg gattgcttcc
    17581 gtcattcgcg aaaatgaagg caaaaaagtt tacattggtg gaggagagga aagttacggt
    17641 ttcttgccgg aagattttgt tcgcgacaaa gatgccgttt cctgctgcgc tttgatggct
    17701 gaaattactg catgggcaaa agatcaggga aaatctcttt ttgaactttt gcaggatatt
    17761 tacctggaat acggatacgg aaaagaaaaa ggaatttcca ttgttaaaaa aggaaaatca
    17821 ggtgcggatg aaattcagga aatgatgcgc aacttcagaa atcatcctcc caaagaaatt
    17881 gccggctcaa aggtttctat tatcaaagat tttcaaactt tgaaaatgtt aaatctaaaa
    17941 accaatgaag aaaactcatt gaatatgcct gccacatcga atgtattgca gttttttacg
    18001 gaagatggca ccaaaatatc ggttcgtcct tcaggtacag agcctaaaat caaattttat
    18061 attgaagtta ggggcgaaat gaaatctcga gccgattttg acaaggctga aactgaagct
    18121 ttaaagaaaa ttgaagctgt tcgcgcttcg ttaggaattt aacgatattg attttttaga
    18181 atattgaaag cgtcattcta gataattatt aacgaatgac gcttttttac cttttacctt
    18241 tcatgcaaaa ttttagcaga atgggaatgg ataccggctt tcagcaacga tatttaaact
    18301 acaatcaggt tttgaaatca gaatttgatg aacgcattca aaaaatttcc gttcatgccg
    18361 gattcacttg tcccaatcga gatgggacaa aaggctgggg tgggtgtact tactgcaaca
    18421 atcagacgtt tgtacccgaa tactgtaaac cggtgaaaag tatcacccaa caactcgatg
    18481 aaggagtaaa tttttttcgg cataaataca aaagtcaata ttatttagct tattttcaat
    18541 catatacaaa tacatacgat agttttgaaa atctgaaatc gctgtacgaa gaagctttgc
    18601 atcatccaaa aataaaaggt ttggtaatcg gcactcgtcc cgattgtgtg agtgaagatg
    18661 tattggatta tttcgaaaga atttctaaaa acagctatgt tcttattgag tacggcattg
    18721 aatcaacttc ggatgctaca ttggaattta ttcgtcgcgg gcatgatttt gcttgtgccg
    18781 aatttgcggt gaaggaaact gctcgcagag gcatcaaaac cggtgctcat ttgattctgg
    18841 gacttccatt tgaaagtaag gaaataatac ttgaacatgc cgataaaatt tcccgattgc
    18901 ctttgactgc attgaaactt catcaacttc agatagtgaa aggaacagtg atggcggttc
    18961 aatatcgtca acatcccgag tggtttcaac tttttactgt tgacgaattt gtcgatttgg
    19021 tggtagattt tctggagaga ctttctccaa acatcgctgt cgaacgattt gtttctcaat
    19081 cgcccgaagg gttggtgata gccccgcaat ggaaaataaa aaattttgaa atagcctcaa
    19141 aaatagagaa acaactcatc aagcgaaata cttttcaggg aagatgctgg aagggaaacg
    19201 attgaaaaac agatacctga gtacaagata taaatatcag tgaagagctt acttataatg
    19261 tacactcttc tctgatattt gtgttttttt tagtgaacga ttattaacgg aaaactgacg
    19321 atgtctttct ctgtctttat tttcagcaca taagttccgg cagcgacgtt gctgatatcc
    19381 aaatttgttt tatttgtctc tttcaataat tttccttgca aagaataaag ctgcatttga
    19441 gaaatgaatt ggtcggaagt tttaaccatt accatgtttg aagcaggatt cggataaatc
    19501 agtattttat ttttcaatac ttgcggaata gccattattg gctcatcata aagaagcata
    19561 ttatcaaaat aaatttctcc attaccagac aaaatccctt catttctaac tactctaaaa
    19621 cctgtaaatt catatggcag cccttcttgt aaatggtcta atttagtttc tacaaattcc
    19681 caaccaaaga aattcaaatc acacaactta acatatttga cttctgcacc tgcaacaaat
    19741 cgaagttcta tcgagtttcc cgataaatct ccatacacat gaaggccgac taccttatca
    19801 tgtgtagcaa caaatccaag tgttgttgcc aggtaagttg cataggcttc tggttctgaa
    19861 aaactgtagc tgaataaata agaggaagct tcaaaaagtt ttttagaagt attacgagac
    19921 acggaagcag aagaaacatt taaacttt
//
LOCUS       DAOSCH010000027        18827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2047_length_18827_cov_5.940550, whole genome shotgun sequence.
ACCESSION   DAOSCH010000027 DAOSCH010000000
VERSION     DAOSCH010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18827
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2047_length_18827_cov_5.940550"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            372..3620
                     /locus_tag="PLZ54_03540"
     CDS             372..3620
                     /locus_tag="PLZ54_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ66040.1"
                     /translation="MTAYFNIYFNGLESYNEGLKNILVAHQDDYSTILPMFPISKHSN
                     ASAATANMDRAIEKSRKAIKLHSIKQKPEKNYNKWNSAEYQAFYNQEEFNPVLKDAWM
                     LIGKAEFHKGDFLGSAGTFSYIARHFASDPDLVAQAKIWLARAYGEMGWLYEAQQVLG
                     DVNQDHLKRQHIGLFASVNADILLKNHQYKEAIPFLRVAIDKETDKKLKQRFLFILAQ
                     LYQKTGDEKAAYETYTKVLQSNPPFEMDFYARIQRSELKAANHAEVQKDLNKMLKSSK
                     YKDYQDQIYYVLGKNFLHRGDTAQAINAFRSSIEKSTRNGYDKANSLITLGDLFYHQK
                     NYKEAYPCYDAASKIIAEEHPDYRRISKLADILSEFVTQYDIVMLQDSLQRLAAMPEK
                     QRMEVIQKIISQVEKEEKAAAKKAEEELLGLNYPNNDDEETLIPIGAQMGNSNDWYFY
                     NQNLMKVGKSEFQKKWGNRKLEDNWRRANKSASLFAEEPNAENVSSEMMMDSTSSTLA
                     ITDNKNPAFYLKQIPLTPLQLKISNEQMAQAMFNVGKIYYEKIQDIPSAIRTFEEFVG
                     RFPDHKLVPDAYFQLYLMESKLGNLANAEKWRNRLVGEFLQTDYSKMLSKPDYIQRLQ
                     RMYQEQDSIYNATYRAYSSNDFATVFRNFQYMQENYPLSELMPKFQFLYALSVGKTQE
                     SQNFEKELDLLVSKYPESDVSTMAKDILALMRQGMVAKSGTTHGSLLAKREEAEKLEI
                     SPSAQQQFSTDTKTKHRLLLITDAPREELNKLMYQIASFNFSRFLIKDFDLGIQPLDS
                     VRNILYVDNFDSYDEVVWYENTMASDAYLTELLSRLKVQELIISDENFALLKNGLSLA
                     DYMAFHDKFLGKNAGSASKQISALPEKEKKSESPKTIAATANKPNSVTETKKTVSTST
                     EKTPLVAPTQPAEQTPNVASVAEQPVKNEPVEKVEVKKEEEVPLFKNLFAYKPNDPHF
                     IAVYVVSGTVDVAKTKAAFDEYNRQNYDVLQLNVRFENVGKQQFFIIGSFTDANTAKS
                     YLLRMVKESKLFEGLKGASYRNLVGTQQNLNVMVQQNALNVYFEFMKQYYLN"
     gene            3652..5199
                     /locus_tag="PLZ54_03545"
     CDS             3652..5199
                     /locus_tag="PLZ54_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOJ66041.1"
                     /translation="MRKERILWIDDEIEMLRPYLLFLDEKGYEVVTATNGNDAVEICQ
                     EENFDIIFLDENMPGLSGLETLALIKEIDPDVPMVMITKNEEENIMNMAIGNKIADYL
                     TKPVNPSQILMTLKKNIHRKEIVNEHATAAYRSEFGQIGMKINDSFTYDDWVEVYKKL
                     VFWELELSETESGMDELLRMQKMDANTTFSKFIKNNYERWFERPEERPLMSFEIFKNK
                     VFPLLDNGEKVFFVVVDNFRLDQWKMIRELLSDYYQFDEEDLYCSILPTATQYARNAI
                     FSGLMPLQIQQMFPQFWVEEEEEEGKNLMESELLQTQIQRYRKKYTFSYHKINDSVSC
                     QHLIDQLPHLENNNLNVCVLNFIDMLSHARTESKMVRELANDEKAYRSLTLSWFRHSA
                     TFDLFRAIAERGYKVVFTTDHGTIRVNNPIKVIGDKNTNVNLRYKVGKNLSYNKKEVF
                     EITQPAKIGLPSPNVSSAYIFACKDDFFAYPNNYNYYVSYYKNTFQHGGVSLEEMLVP
                     LIVMNPK"
     gene            5310..6062
                     /locus_tag="PLZ54_03550"
     CDS             5310..6062
                     /locus_tag="PLZ54_03550"
                     /inference="COORDINATES: protein motif:HMM:NF013293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ66042.1"
                     /translation="MRIGLIGFGKTGKAVASVILQSESFELKWILRRKKLLDHRSAAE
                     VLGIETSSTEGLIYSTSNTNLENLMDTLPVDAIIDFSSGQGIYTYGELAAKRKIKIIS
                     AISHYKPKEVAFLHKLAQQTTVFWSPNITLGVNYLMISAKFLKKIAPWVDIEIVEEHF
                     KGKDGISGTALKIAEVLDMDKAKINSIRAGGIVGRHEIIFGFPYQTVRLIHESISREA
                     FGNGVLFVAENLKDKEDGFYNFQDLLLPYFYD"
     gene            6152..8191
                     /locus_tag="PLZ54_03555"
     CDS             6152..8191
                     /locus_tag="PLZ54_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOJ66043.1"
                     /translation="MKKILFVLMTVAFLGCRSEKPATPAMETFPYTVDRFADIEILRY
                     RVPDFETLTLQQKELIYYLSEAALSGRDILYDQNNKFNLCVRRTLEAVYENYRGDRQS
                     EEFRNFITYLKQVWMGNGIHHHYSQDKFVPKFSAAFFAQAVKSIDAEVLPLEKGETPD
                     DLIRKLTPVIFDPTVFPKRTNQADGVDLLLTSAVNFYEGVTQAEAEEFYDRMKNPNDS
                     TPISYGLNSKLIKENGVLMEKTYKLGGMYGKAIEQIVFWLNKALSVAENDKQKDVIHS
                     LIKFYETGDLKTYDEYCIKWVQDTASLVDFVNGFTETYADPLGLKGSWESIVNFKNLA
                     STKRTETISENAQWFENNSPIDNRFKKENVKGITAKVVTAAILAGDCYPSTPIGINLP
                     NSDWIRHDYGSKSVTIENITEAYDRASLGNGFNEEFMWSDVERERAKKYGSLTNNLHT
                     DLHECLGHGSGKLLPGVDPNGLKAYASTIEEARADLFGLYYVADSKMLELGLLPDHEA
                     YKAEYYKYIMNGLLTQLTRVQTGKNIEEAHMRNRQLIARWVYEKGLPENVIELKKRDG
                     KTYVVVNDYEKLRILFGKLLAEIQRIKSTGDYNGAKTLVENYGVKVDPVIHKEILERY
                     EKLNLAPYRGFVNPVYKAIKNADGEIRDVKISYDENYVEQQLRYSRQYSNLPTFN"
     gene            8405..9562
                     /locus_tag="PLZ54_03560"
     CDS             8405..9562
                     /locus_tag="PLZ54_03560"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOJ66044.1"
                     /translation="MNTNNEKWHGSPKKGGNSYDSREGRQFEKGGNNSPFSKPGRNQK
                     VYSSRNTSNSNYDNRSTGGKPYYKNENKKPYRRPFTSDENTEGENRKLKLVKPGKFVE
                     TRFDDRQPNVTFRKRKNKTEGEFNPNDKYSLKKQLEYQKNNVDYSKPIRLNKFLANAG
                     ICSRREADEFIQSGVISVNGEVVTQLGAKIMMTDTVMFKNQPVQSERKVYLLLNKPKD
                     CLTTSDDPNNRKTVLDLVKNACSERIYPVGRLDRNTTGVLLLTNDGDLAAKLTHPKYN
                     KKKIYHAVLDKNLETVDYESIIQGVDLGDEHVSVDALEFVKAGNRREIGIEIHSGQNR
                     VVRRIFEKFGYKVVYLDRVYFAGLTKKNVPRGKYRFLTQREINMLKMGAYE"
     gene            9639..10205
                     /locus_tag="PLZ54_03565"
     CDS             9639..10205
                     /locus_tag="PLZ54_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66045.1"
                     /translation="MSEENLNNTRSDEQSPVSSQPTNKKGFFRTFISRHTGVFVLLCL
                     LIAVVIAAFIRIKLLERSFNKQQEALRVRYENKVDSLLQADRLTTAKVFSWAVRGELI
                     RDNRDQINQYFMNFVKESGVKKIKLIDPVSAKAIISTDKKDEGLVFADPTFFTEEIAV
                     RHLDSVTQIISPVMGLNKKIGILVIEYE"
     gene            10401..13628
                     /locus_tag="PLZ54_03570"
     CDS             10401..13628
                     /locus_tag="PLZ54_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOJ66046.1"
                     /translation="MNDLMKRFKSLNVIFGWVAFAIATVTYLLTLEPTASFWDCGEFI
                     STAYKLDVGHPPGAPFFMLTARFFTLFTSDPTRVAVMVNTLSALCSGLTILFLFWTIT
                     HLARKVVIKSDEDYTTGNIIIILGAGLVGALAYAFSDTFWFSAVEAEVYAYSSLFTAV
                     VFWLILKWEEVADEPGSDRWLLLIAYLMGLSIGVHLLNLLAIPAIVLVYYFKKFTPSL
                     KGVLFALLGSFLILGVVLYGIIPGFVQVAGWFELLFVNKLGMAFNSGLYVYIVLSIAA
                     LTWAIYESYVNKNYLRIVLSFLVAITLVGIPFFGQHIVLGIILVAGLSFYFYKWKNKI
                     NVHWIHTSILMITLMLIGYSTYSVIVIRSAANPPMDQNSPDNVFSLRYYLNREQYGDR
                     PLIYGPVYNAPVKLRIDGNMCIPEQELGEAMYAPKPKKSPNEKDQYMLTGYKMDYKMD
                     ERFNMLFPRMYSTNSSHVQAYKIWGNVKGKPITFDYCGQQRTEMKPTFAENLRFFFDY
                     QLNFMYWRYFLWNFSGRQNDLQGNGEVDKGNWITGINFIDKYLVGDQTNLPTFLAENK
                     GRNAYFMLPLILGILGILWQLYGGKNGKQTFWVTFLLFFMTGIAIVVYLNQTPYQPRE
                     RDYAYAGSFYAFSIWIGLGVLEIFKFINKYIPKTPSAILASLLCLGVPVLMASENWDD
                     HDRSGRFTARDFGQNYLESCRPNAIIFTNGDNDTFPLWYNQEVEGKRTDIRVCNLSYL
                     QTDWYIDQMKREAYESAPLPISWEPEDYISGKNEVISVEDFIKQPIDVKTIMDFIRSS
                     DPQTKINGEAVIPTKQIYIPVDAQQVVKTGTVKPERAAEIVPKIDFNLKNRLTKSELM
                     VIEMLKENNWERPMYFAVTVGDDYYLGLKDHFELTGMAYQIVPVGAKDAGPGVNTDEM
                     YENVMHKFRFGGINNPKVYIDENIGRMCRTHRMFMGQLAGALLAEGDTVKAKAVLDYC
                     LEMIPGNSVRHDYTSVQIANYYYRLEEFEKGDAIMDSVAKDCVEYINWGFSLSPSKQN
                     SITGDLGNNMAVLSQILQICDELKRKNILDKYLPVYMQYSRYFQM"
     gene            13629..14237
                     /locus_tag="PLZ54_03575"
     CDS             13629..14237
                     /locus_tag="PLZ54_03575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOJ66047.1"
                     /translation="MSCQFPAFLRPLIGRNYLWRKNSSEKVVYLTFDDGPVPEVTPQV
                     LGVLDDFDIKATFFCVGDNVKKYPELYREILLKGHSVGNHTFHHLNGFKVGQEQYLKD
                     VEQASQLIDSRLFRPPYGKLTRKEKKILEQKFQIVLWDVISYDYDQNLSSSQVLKNVK
                     RYTRSGSVILFHDSVKAKNNMLTVLPQAISFLLAKGYRFEKL"
     gene            14307..15584
                     /locus_tag="PLZ54_03580"
     CDS             14307..15584
                     /locus_tag="PLZ54_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOJ66048.1"
                     /translation="MKESILQYVWQHKLFLQSNLQTADGEKVEIIDVGKSNTDAGPDF
                     FNSKIKIDGTLWVGNVEIHLRSSEWIKHGHQKDKAYDTVILHVVAEMDGEVFRTDGTK
                     IPQLVLPFSKDIEANYDSLLNNNKWIPCADKIEKISEIFIQSWKNALLVERLEQKMTE
                     IENLLTQNHYHWEEAFYIIIARNFGFGTNGQAFELLAKSVPLNVLAKHKDNLLQLEAI
                     LFGQAGLLPVDTSDDEYAMQLIKEYAFLRNKFSLQPIDCSQWKLFRLRPYNFPHVRIA
                     QFASLIHQSSKLFSKIIETPNLELMKDLFSCKPSNYWQTHYLFGSASPKSTKQLGLYS
                     IYSIVINTIVPFLFCYASKKNNEDLKDKSLHLLEAIPSEKNAIVKGWRNIGIASESAF
                     DSQALIQLKKQYCDTKKCLFCRIGHKVLTLQTT"
     gene            15597..17432
                     /locus_tag="PLZ54_03585"
     CDS             15597..17432
                     /locus_tag="PLZ54_03585"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ66049.1"
                     /translation="MRLNFFKTIAFSGIILSVVGCANKAQGPTGGPKDSIPPSVVRSV
                     PSNGALNFKKKQILIDFDENVSIEKAVENVLISPPQVKPPEVVAIGKRVTVNLQDDLK
                     DSTTYTINFGNAIVDLNEKNPLKNFSFSFSTGNQIDTLQVKGTVINAEDLNPVSGVIV
                     GVYNDLSDTVFTQKPFLRIGKTDEIGKFSVENLKKDTFKIYALGDANRDYFYQPGEGL
                     AFTDSLIVPGFFREEVMDTIWKDSVQIDTIRSIVRTHFFPDNILLKYFKENKKRQYFI
                     KAERTLPYAFSLFFSAPMTELPTLKPIDFEWDGKYILQKNNTMDSLTYWMTDSLVWKK
                     DTLQFEMSYQKTDSLFRLVPATDTLKIFMRTARSTVRNTKDKKEPVSRVKMEQNYQFT
                     TNIAGSFDIYQPVVFRFSTPLAYFDLSKISLAQRVDTILKPLPVNWRQVDSTKMVYAV
                     DYKWEPEKNYELTVDSAAFTSIYQQTSKKYSGKFKIRSLDEYSTVIMVLENFVPNAVI
                     QILDAKDAVIASKQAQEKGTVFQYLKPGDYYARLFLDENGNGKWDTGDFAAHRQPEAV
                     FYYPKKMTLMPNWEFEETWDYLKVPLLEQKPKEIRKDAGKKVSGN"
     gene            complement(17474..18703)
                     /locus_tag="PLZ54_03590"
     CDS             complement(17474..18703)
                     /locus_tag="PLZ54_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOJ66050.1"
                     /translation="MKKLLLLAILGFGIVSCNDETESASQLPPDAKPITLTDGQAKRV
                     GQDNEFALDLFRKVINFSKETNVFISPLSVSIALGMTWNGAAGETKSEMESALKMSGM
                     SVEEINEYYRILQTTLPTIDPTTKLTLANSLWYRSGFQVKQDFLKVNIDYFNAYVREL
                     DFSKAWAVDTINQWCAKKTNNLIPSVLDAIPDEAVMYLINAIYFKGIWRHQFEKKNTK
                     QTDFTNEAGEKVKVNMMFQKDTFAYAEDQKAQYLDMPYGNKAFSMTVILPKNGNTTAD
                     LLEYLTVDQWNSILQNLSMREVEVYFPRFSSKNKFLLNDPLIDMGMKLAFTDNADFTN
                     IANENLFISRILHNTYIEVTEEGTEAAAVTVVEVGYTSVPIIPEFRVNKPFLFVIREN
                     STGVILFMGKMGNMELF"
BASE COUNT         5898 a         3273 c         3912 g         5744 t
ORIGIN      
        1 aagagaagct ccggggtatc caacagaaat gttgatcctc tggagctttt ttcttgtatt
       61 aaagtattat ctgtgtgttt ttttctgaaa tgaatatgat tttatggcta cacaatttat
      121 gttgaatggt gaaaaattaa aaattttatg taagtttgta tgttgtaaaa taatagaatt
      181 tcaatttttt tgtttttgtt ttagtggaga atatttttta ttaaacaatc tcttatatta
      241 ttgattaatt tgattcacga taattgttga aaaggaaaaa tttaaataaa atactgtttc
      301 tgctgatcat aatttttgtg agcagttgtt caatgcagaa aaacacatgg ttttctcgta
      361 attatcatgc gatgactgct tattttaata tctatttcaa tgggctggaa agttacaacg
      421 agggattgaa aaacattttg gtggctcacc aggacgatta ttccactatt cttccgatgt
      481 ttcccattag taaacattcc aatgcttcgg ctgctacggc aaatatggat cgtgccatag
      541 aaaaatcgag aaaagccata aagcttcact ccatcaagca aaaacccgag aagaattaca
      601 ataaatggaa ttctgccgaa taccaggctt tttataatca ggaagaattc aatcctgttt
      661 taaaagatgc atggatgctt attgggaaag ccgagtttca taaaggcgat tttttgggtt
      721 cggccggaac attttcctat atcgcccgcc attttgcttc tgatcctgat ttggttgcac
      781 aagccaaaat ttggttggct cgtgcttatg gtgaaatggg ttggctttac gaagctcagc
      841 aagttttggg agatgttaac caagaccatt taaaacgcca gcatatcggt ctttttgctt
      901 ctgttaatgc agatattctg ttgaaaaacc atcagtacaa agaagcgatt ccttttttga
      961 gggttgcaat tgacaaggaa accgacaaga aattgaaaca acggttcctt ttcattttgg
     1021 ctcagcttta tcagaaaacg ggcgacgaaa aagcagccta cgaaacctat accaaagtgc
     1081 ttcaatccaa tccgcctttc gaaatggatt tttacgcacg cattcagcgc tcggaactga
     1141 aagcagccaa ccatgcagaa gtgcagaaag atttgaacaa aatgcttaaa agttccaaat
     1201 ataaagatta tcaggatcag atttattatg ttctcggcaa aaatttcttg catcggggcg
     1261 acacggctca ggccataaat gctttccgtt cctctattga aaaaagcacc cgaaatggtt
     1321 atgataaagc caattctctg attacgctcg gagatttgtt ttatcatcag aagaattaca
     1381 aggaagcata tccctgctat gatgctgctt ccaaaattat tgctgaagaa caccccgact
     1441 accggcgtat ttcaaaatta gccgacattt tgagtgaatt tgtaacgcaa tacgatatag
     1501 taatgttgca ggacagtttg cagcgtttgg ctgccatgcc cgaaaaacag cgtatggaag
     1561 tgattcaaaa aatcatcagt caggttgaga aggaagaaaa ggcggctgct aaaaaagctg
     1621 aagaagagtt gctgggattg aactatccca ataatgacga tgaagaaacc ctcatcccaa
     1681 ttggtgctca aatgggtaat tccaacgact ggtattttta taatcaaaat ttgatgaaag
     1741 ttgggaaaag tgagtttcag aaaaaatggg gcaaccgaaa attggaagac aattggcgtc
     1801 gtgccaataa atcggcttcg ctttttgcag aggaacctaa cgccgaaaac gtcagcagtg
     1861 aaatgatgat ggacagcacg tcatcaacat tggccatcac cgacaataaa aatccggcat
     1921 tttatctgaa acaaataccg ctgacgccgc ttcaattgaa aatttcaaat gagcagatgg
     1981 cgcaggcaat gtttaatgtg ggaaaaattt attatgaaaa gattcaggat attccttctg
     2041 caattcggac ttttgaggaa tttgtcggaa gatttcctga tcacaagctc gttccagatg
     2101 cttattttca gttgtattta atggaatcta aactcggcaa tctggctaat gcagaaaaat
     2161 ggcgcaacag acttgtgggt gaatttctac aaaccgacta ttcaaaaatg ctttcaaaac
     2221 cggattatat tcaacgtttg caacgaatgt atcaagagca ggattcgatt tacaatgcta
     2281 catatcgggc ttattcgagt aatgatttcg ctactgtttt ccgaaatttc caatatatgc
     2341 aagaaaatta tcccttgtcg gaattgatgc ctaaatttca gtttctttat gcgttgagtg
     2401 tgggcaaaac acaagaatcg caaaactttg aaaaagaact ggatctgttg gtttcaaaat
     2461 atcctgaaag tgatgtcagt acaatggcga aagatatttt ggcgctgatg cgtcagggga
     2521 tggtagcaaa atccggtact acacacgggt cattgcttgc caaaagggaa gaggcggaga
     2581 aattggaaat ttcgccatct gcgcagcagc aattttctac agataccaaa accaaacata
     2641 ggcttttgtt gattacggat gctcctcgag aagaactgaa caagttaatg tatcagattg
     2701 cttccttcaa cttttcccgt tttctcatca aggattttga tttgggaatt caaccgttgg
     2761 acagtgtcag aaatatatta tatgttgaca acttcgattc ttacgacgaa gtggtttggt
     2821 atgaaaatac aatggcttcc gatgcttatt tgactgagtt gctttcacga ttgaaagtgc
     2881 aggaattgat aatttccgat gaaaattttg ctttgttgaa aaatggatta tcgttagcgg
     2941 attatatggc ttttcatgat aaatttttgg gcaaaaatgc cggctctgct tccaaacaaa
     3001 tatccgcgtt acctgaaaaa gaaaagaaat ctgaatctcc caaaactatt gcggcgacgg
     3061 caaacaaacc caattcggta accgaaacaa aaaaaacagt ttcgacttcg accgaaaaaa
     3121 ctcctttggt tgcaccaact caaccggcag aacaaacgcc aaacgttgct tctgttgccg
     3181 aacaaccggt gaaaaacgag cctgttgaaa aagttgaagt caagaaggaa gaagaagttc
     3241 cgctgttcaa aaatctgttt gcctataaac cgaacgatcc acatttcatt gccgtttatg
     3301 ttgtatcggg aacggtagat gtggcaaaaa ccaaagcagc ttttgacgag tacaaccgac
     3361 aaaattacga tgtgctgcaa ctgaatgtgc gatttgaaaa cgtgggcaag cagcaatttt
     3421 tcattatagg cagcttcacc gatgccaata ctgccaaatc gtatttgttg cgtatggtga
     3481 aggaaagcaa actgtttgaa ggattaaaag gtgcaagtta cagaaatctg gtcggaacgc
     3541 agcagaacct gaatgttatg gtacagcaaa atgcgctgaa cgtatatttc gagtttatga
     3601 aacaatatta tctgaactga aaagtttttt gagaaaagga aattgaagga aatgagaaaa
     3661 gagagaatac tttggataga tgacgaaatc gagatgctgc gaccttatct cctttttttg
     3721 gatgaaaagg gatatgaagt tgttacggct accaatggga atgatgccgt agaaatctgt
     3781 caggaggaaa atttcgatat tatttttctg gatgaaaaca tgccgggatt gagtgggctg
     3841 gaaacgctgg cactgattaa ggaaatcgac ccggatgttc caatggtgat gattacaaaa
     3901 aatgaagaag aaaacatcat gaatatggct attggcaata aaattgccga ttatttgaca
     3961 aaacctgtca atccaagtca gattctgatg acgctcaaga aaaatattca tcgaaaggaa
     4021 atcgtaaacg aacatgcaac agctgcctat cgttccgaat ttggacaaat aggcatgaaa
     4081 atcaacgatt catttacata cgacgattgg gtagaagttt acaaaaaact tgttttctgg
     4141 gaattggagc tttccgaaac cgaaagtggg atggatgaat tgcttcgaat gcagaaaatg
     4201 gacgccaata caactttttc aaaattcata aaaaacaact acgaacgatg gttcgagcgc
     4261 cccgaagaac gtcctttgat gagttttgaa atatttaaga acaaagtttt tcccttgctt
     4321 gataatggcg aaaaggtttt ttttgtggtg gtagataatt tccgcctcga tcagtggaaa
     4381 atgatacgtg aattgttgag cgattattat cagtttgacg aagaagatct ttattgcagt
     4441 attcttccta ccgctacaca atatgcgcga aatgccatct tttccggtct gatgcctctg
     4501 caaattcaac agatgtttcc tcaattttgg gtggaagaag aagaggaaga aggtaaaaac
     4561 ctaatggaga gtgagttgct tcagacgcaa attcaacgct atcggaaaaa atatactttt
     4621 tcgtaccata aaattaacga ttccgtttcg tgtcagcatc tgatcgacca gttgccacat
     4681 ttggaaaaca ataatttgaa cgtttgtgta ctgaatttta tcgacatgct ttcgcatgca
     4741 cgtacggaat caaaaatggt tcgcgagttg gccaacgacg aaaaagctta tcgctcactc
     4801 accctttcat ggttccgtca ttctgctact ttcgatttgt tcagggctat tgccgaacgc
     4861 ggttacaaag tggtttttac aaccgatcac ggcaccattc gagtaaataa tcccataaaa
     4921 gtcattggag ataaaaatac aaatgtcaat ctgcgctata aggtagggaa aaatttgagc
     4981 tacaacaaaa aggaagtttt cgaaattacc caacctgcaa aaattggctt accaagcccc
     5041 aatgtcagtt cggcttacat atttgcctgt aaggatgatt tttttgctta tccgaataat
     5101 tacaattatt acgtgagtta ttataaaaat acctttcaac atggcggcgt ttctttggaa
     5161 gaaatgttgg tgcctctaat tgtgatgaat cccaaataag tggatgaagt taaatctttt
     5221 tcgacagcgg ttgaaaaaga gtaatttgca gtaatatttt tatttataaa cgacatcagg
     5281 tgaagactct gatgaaaaaa cgaaatacta tgagaatagg attgatagga tttggaaaaa
     5341 cgggaaaagc cgttgcttca gttattttac aaagcgagag ttttgaactg aaatggattc
     5401 tcaggcgtaa aaaactactc gatcaccgtt ctgctgctga agtcttgggt atagaaacca
     5461 gcagtacgga aggacttatt tattcgactt caaatacgaa tctcgaaaat ttaatggata
     5521 ctttacccgt agatgccatc attgattttt cttcaggtca aggtatttat acttatggag
     5581 agctggcggc aaagagaaaa attaaaatta tctcggctat ttctcattac aaaccgaaag
     5641 aagttgcttt tttgcataag ttggctcaac agactaccgt tttctggtcg ccaaatatta
     5701 cgcttggagt aaactattta atgatttctg ccaaattttt gaaaaaaatt gccccttggg
     5761 ttgatattga aatagtcgaa gagcatttta aggggaaaga tggaatttca ggtactgcct
     5821 tgaaaattgc tgaagtgctg gatatggata aagcaaaaat caattccatt cgtgcagggg
     5881 gaattgttgg ccgacacgaa atcatttttg gatttcctta tcagaccgtc agactgattc
     5941 atgagtctat ttcgcgggaa gcttttggta atggagtttt gtttgttgcc gaaaatttaa
     6001 aggacaagga agatggtttt tataattttc aagatttatt gcttccttat ttttatgatt
     6061 aatcaaaaag ttgcattttc aacttgaatg ttgcaataga aaaaatcaaa ttttttctat
     6121 taacttgaaa tacgaagaaa ttttgaaaag tatgaaaaaa attttatttg tgcttatgac
     6181 agtagctttt ttaggatgtc gttccgaaaa accggcaacg ccggcgatgg aaacatttcc
     6241 ttacacggtt gaccgatttg ccgatattga aattctgcgt tatcgggttc ccgatttcga
     6301 aacactaact ttgcaacaga aagaactcat ttattatctt tcagaagctg ctttatcagg
     6361 gcgcgacatt ttgtacgacc agaacaacaa gttcaattta tgtgttcgtc gtacattgga
     6421 agctgtttac gaaaattatc gcggagatcg ccagtctgaa gaattcagaa attttattac
     6481 ttatctgaag caggtttgga tgggcaacgg tattcatcat cactattcgc aggataaatt
     6541 tgttccaaag ttttctgcgg ctttttttgc tcaggcagtg aaaagtattg atgctgaagt
     6601 tttgccgctc gaaaagggcg aaactcccga tgatttgatt cgtaaactga cccctgtgat
     6661 ttttgatccg actgtttttc ctaaacgtac caatcaagct gacggagtgg atttattgct
     6721 tacttcggca gttaattttt atgaaggcgt tacacaagct gaagctgaag aattttacga
     6781 ccgaatgaaa aatccgaatg attcaactcc catctcttac ggactaaaca gtaaactgat
     6841 caaagaaaat ggtgttttga tggaaaaaac ttataaattg ggaggaatgt acggaaaggc
     6901 cattgagcaa attgttttct ggctcaataa agctctttct gtggccgaaa atgataaaca
     6961 aaaggacgtc attcattcat tgataaaatt ttatgaaacc ggagatttga aaacctatga
     7021 tgaatattgt ataaaatggg tacaggatac tgcgtcactg gtagattttg taaacggctt
     7081 cacggaaacc tatgccgatc cgcttggatt gaaaggcagt tgggaatcta ttgtgaattt
     7141 taaaaatttg gcttccacca aaagaactga aaccatcagc gaaaatgcgc agtggtttga
     7201 aaataattcg cccatcgata atcgttttaa aaaggaaaat gtgaagggaa tcacagcaaa
     7261 agtagttact gcagccattt tggccggcga ctgctatccg tccactccga ttggcattaa
     7321 tcttcccaat tccgattgga tcaggcacga ttatggttca aaatcggtta ctattgaaaa
     7381 cattacggaa gcttacgacc gggcttcttt gggaaacgga ttcaacgaag agtttatgtg
     7441 gagcgacgtg gaacgcgaaa gagctaaaaa gtacggttcg ttgaccaata atttgcacac
     7501 tgatttgcat gaatgtctgg ggcatggttc cgggaaattg cttccgggcg tcgaccccaa
     7561 tgggttgaaa gcttatgcat cgaccattga agaggcacgt gccgatttat tcggattgta
     7621 ttatgtggct gattcaaaaa tgctcgaact cggactgttg cccgaccatg aagcctataa
     7681 agccgagtat tataagtata tcatgaatgg ccttctcact cagctgacgc gcgttcagac
     7741 agggaaaaat attgaagagg ctcacatgcg caatcgtcaa cttattgctc gctgggtgta
     7801 cgaaaaaggt ttgcccgaaa atgtgattga acttaaaaaa agagatggaa aaacttacgt
     7861 tgtggtcaat gattatgaaa aactgagaat tctttttgga aaacttctgg ccgaaattca
     7921 gcgcattaag tccaccggcg attacaatgg tgcaaaaact ttggtagaaa attacggagt
     7981 aaaagttgat cctgttattc acaaggaaat tttggaacgc tacgaaaaat taaatcttgc
     8041 accttatcgc ggctttgtca atcctgttta taaggctata aagaatgccg atggggaaat
     8101 cagggatgtg aaaatttctt atgacgaaaa ttatgtggag caacaattga ggtattcaag
     8161 acaatattcc aatttaccga cattcaattg aaaagcattt ttttgtaata gtttgttgaa
     8221 taatagttta ataaaatttt aaaggaattt ttttcgtaag ggaacttact tttttggtta
     8281 ttgtcgccat gacaggaagt aagtgtaaca attgttgtgg ataatgccta aatttggcta
     8341 aaaaactgtt gatatttaag gaatttttcc gaactttgca gcctaaaaat gaataacatt
     8401 ggaaatgaat acgaacaacg aaaaatggca tgggagtcca aaaaaaggag ggaactctta
     8461 tgacagccgt gagggcagac agtttgaaaa aggcggcaat aattcgcctt tcagtaaacc
     8521 gggaaggaac caaaaggttt attcttctcg aaatacctcc aattccaatt atgacaatcg
     8581 ttctaccgga gggaaaccct attataagaa cgaaaacaaa aaaccataca ggcgaccgtt
     8641 tacaagtgat gaaaatacgg aaggtgaaaa tcgcaaattg aaactggtaa aacctggtaa
     8701 gtttgttgaa acgcgttttg atgaccgaca acccaatgta acttttcgta aacgaaaaaa
     8761 caaaaccgaa ggcgagttta atcccaatga taaatacagt cttaaaaaac aactcgagta
     8821 tcaaaaaaat aatgtcgatt attcaaaacc tattcggttg aacaaatttt tggccaatgc
     8881 aggtatttgt tctcgccgcg aagccgatga atttattcaa tcgggtgtaa tttctgttaa
     8941 tggtgaagtg gttactcagc tcggtgcaaa aattatgatg accgacacag tgatgtttaa
     9001 aaaccagccc gtgcagtcgg aaagaaaagt gtacctattg ctcaataagc cgaaagattg
     9061 cctgacaact tctgatgatc ccaataaccg caaaacagtg ctcgatttgg taaaaaatgc
     9121 ttgcagtgaa aggatttatc cggtcggaag gctcgacaga aataccactg gtgttttact
     9181 gctcactaac gatggtgatt tggcagccaa attgacccac ccgaaataca acaaaaagaa
     9241 aatttaccat gccgttctcg acaaaaatct tgagacagtc gattacgaat ctattataca
     9301 aggtgtagat ttgggagatg aacatgtgtc ggtcgatgca ctcgaatttg taaaagccgg
     9361 caatcgaaga gagataggaa tagaaattca ttcaggacaa aacagggtag taaggcgaat
     9421 ttttgaaaaa tttggttata aagtcgtata tcttgaccgt gtttatttcg ccgggttgac
     9481 aaaaaagaat gtacccagag gtaaatatcg ctttctgact cagcgcgaaa tcaatatgct
     9541 gaaaatggga gcttacgaat aaaaaagttc ttatgggttt tttcttatga atttttttaa
     9601 aaaaatttgt actacaatca tttaaaacca aatgtgatat gagtgaagaa aatttgaaca
     9661 atactcgttc tgacgagcaa agtcccgttt cgtcgcagcc tacaaataaa aaaggttttt
     9721 tccgaacatt tatttcacgt catacgggag ttttcgtatt gttgtgtttg ctgatagctg
     9781 tagttattgc tgcctttatt cgtattaaat tgttggaacg cagtttcaac aaacaacagg
     9841 aggctttgcg ggttcgatac gaaaataaag tagacagcct tcttcaagcg gacaggctga
     9901 caacggccaa agttttttcg tgggcagtga ggggcgagct gatacgtgat aaccgagatc
     9961 agataaatca gtattttatg aattttgtta aggaatccgg cgtaaagaaa attaaactca
    10021 ttgaccctgt ttctgcaaaa gcaattatct ctaccgataa aaaagatgaa ggtttggttt
    10081 ttgccgatcc cactttcttc actgaagaaa ttgcagttcg ccatcttgat tccgtaactc
    10141 aaattatttc tccggttatg ggactgaata aaaaaattgg cattttggta atagaatacg
    10201 aataaatttc tttttttttt gacatgtaca aaagactgtt taaaattttt tttgatcagc
    10261 ctttttattt ttgttctgaa ttttttttgt ttcaatttta aaaagaagcg gggaaatcat
    10321 ttcgtctttt taaaataaat tttctttgta cctttggatt ttcttaagta ttttcagcaa
    10381 aaaagctaaa aaatttttcc atgaatgatt taatgaaacg attcaaatct ctcaacgtta
    10441 tttttggctg ggtagcattt gctattgcta ctgtaaccta tttgctaact ttggagccaa
    10501 ccgccagttt ttgggattgc ggcgaattta tatccacagc ttacaagctc gatgtgggtc
    10561 atcctcccgg agcaccgttt tttatgctca cggcaagatt ttttacgcta tttacttccg
    10621 atcccacacg cgttgcagtt atggtaaata ctttgtcggc tttgtgcagt ggcttgacta
    10681 ttttattcct tttctggacc attactcatc tggctcgcaa ggttgtgatc aagtcggacg
    10741 aagattatac taccggcaac attatcatca tcttaggtgc cggactggtg ggtgctttgg
    10801 cttatgcttt ttccgataca ttctggtttt cggctgtgga ggcagaagtt tatgcttatt
    10861 cttcactgtt tactgcagtg gttttttggc tgatattgaa gtgggaagaa gttgcagatg
    10921 agccgggttc ggatcgatgg ttgttgctga ttgcttattt aatgggtttg tctattggtg
    10981 ttcacctatt aaatttgttg gcaattcctg ccattgtgct ggtttattat tttaaaaaat
    11041 tcacaccatc tcttaagggt gttttgtttg ctttgcttgg ttcttttctg attttgggag
    11101 tggttttgta tgggattatt ccaggatttg ttcaggtggc cggttggttt gaacttttgt
    11161 ttgtcaataa attgggaatg gctttcaatt cagggcttta tgtttatatt gttctttcaa
    11221 ttgcggcact tacttgggct atttacgaat cttacgtcaa caaaaactat ttaaggattg
    11281 tattgtcttt cctcgtggcc attactttgg taggcattcc gttttttgga cagcatattg
    11341 tgctgggcat tattcttgtt gccggcttaa gcttttattt ttataaatgg aaaaataaaa
    11401 tcaatgttca ttggattcat acttccattt tgatgataac tttaatgttg attggctatt
    11461 cgacttatag cgtcattgtt attcgttctg ccgccaatcc acctatggat caaaattcac
    11521 ccgacaacgt tttttcgttg cgttattatt tgaatcgtga gcagtatggc gatcggccac
    11581 tgatttatgg acctgtttac aatgcaccgg tgaagttgcg cattgatggt aatatgtgta
    11641 ttcctgaaca agagttggga gaagcaatgt atgcaccaaa accaaaaaaa tcgcccaacg
    11701 aaaaagatca gtatatgctt acagggtata aaatggacta taagatggac gaaaggttca
    11761 atatgttatt cccacgtatg tacagcacca acagttcgca tgttcaggca tacaaaatat
    11821 ggggcaatgt aaagggtaaa cccataactt tcgattattg tggacagcag cgcacagaaa
    11881 tgaaacctac ttttgccgaa aatctgcgtt tctttttcga ttatcaatta aatttcatgt
    11941 attggagata ttttctttgg aatttttccg gccggcagaa cgacttgcag ggaaatggag
    12001 aagttgataa gggaaactgg attaccggta tcaattttat tgacaagtat ctggttggag
    12061 atcaaacgaa tcttcctact tttcttgcag aaaataaagg aagaaatgct tatttcatgc
    12121 ttcccctcat tttgggtatt cttggaattt tatggcagct ttacggtgga aaaaatggta
    12181 agcaaacttt ttgggttacc tttctcttat tctttatgac cgggattgcc attgtggtat
    12241 atttgaatca aactccttat cagcccagag aaagagatta tgcttatgca ggttcatttt
    12301 atgcttttag tatctggatt gggttgggag ttttagaaat tttcaaattc ataaataaat
    12361 acataccgaa gacgccaagt gctattcttg cttctttact ttgtttggga gttcctgtac
    12421 taatggcttc cgaaaactgg gatgaccatg accggagcgg acgtttcaca gctcgcgatt
    12481 ttgggcaaaa ttatcttgaa tcgtgccgac cgaatgccat catcttcacc aacggcgata
    12541 atgatacttt ccctctgtgg tacaatcagg aagtggaagg gaaaagaacc gacattagag
    12601 tttgcaattt aagttatttg caaaccgact ggtacataga tcagatgaaa cgtgaagctt
    12661 acgaatcagc tcctctaccc atttcttggg aacctgaaga ttatatttcg ggtaaaaatg
    12721 aagtgatttc ggtggaagat tttattaagc agcctattga tgttaaaacc atcatggatt
    12781 ttattcggag ttccgaccct caaaccaaaa taaatggtga agcggttatt ccgaccaagc
    12841 aaatttacat tcccgttgat gcacagcagg tagtaaaaac cggaaccgtc aaacctgaaa
    12901 gagcagccga aattgttcca aaaattgatt tcaacctgaa aaacaggctg acaaaaagcg
    12961 aattgatggt gattgaaatg ttgaaggaaa ataattggga acgtcccatg tattttgccg
    13021 taacagtagg agatgattat tatttaggat taaaagatca ctttgaactc acaggtatgg
    13081 cttatcaaat tgtgcccgta ggtgcgaagg atgcaggtcc tggtgttaat acggatgaaa
    13141 tgtacgaaaa tgtgatgcat aaattccggt ttggaggaat aaataatccc aaagtgtata
    13201 tagatgaaaa cattggcaga atgtgtcgca cacaccgcat gtttatggga cagctggctg
    13261 gcgctttact tgctgaaggc gacaccgtaa aagctaaagc agtgcttgat tattgtttgg
    13321 agatgattcc tggaaactct gtgcgtcatg attatacttc cgtgcaaata gcaaattatt
    13381 attatcgcct ggaagaattt gaaaaaggtg atgccatcat ggatagcgtg gctaaagatt
    13441 gtgtagaata tataaattgg ggttttagtt taagtccttc aaaacaaaac agcataacgg
    13501 gagacttggg aaataacatg gcagtgctaa gtcagatact gcaaatttgc gatgagctaa
    13561 aacggaaaaa tattcttgat aagtatcttc ctgtttacat gcaatattcg cggtattttc
    13621 aaatgtgaat gagctgtcaa tttcctgctt ttttgcgtcc gctgatagga cgaaattatt
    13681 tgtggagaaa aaattcttcg gaaaaagttg tctatctgac ttttgatgat ggaccggtgc
    13741 ctgaggttac gcctcaggta cttggtgtac ttgatgattt tgacattaaa gctacttttt
    13801 tttgtgtagg cgacaatgtg aaaaaatatc ccgaacttta ccgggaaatt cttttgaagg
    13861 ggcacagcgt tgggaatcac acttttcatc atttgaacgg gtttaaagtt gggcaagagc
    13921 aatatctgaa agatgtggaa caggcttcgc agttgattga cagccggttg tttcgtcccc
    13981 cttacggaaa actcactcgc aaagagaaga aaattttgga acaaaaattt caaattgtct
    14041 tgtgggatgt gatttcgtat gactatgacc aaaatctgtc gtcttcacaa gttttgaaga
    14101 atgtgaaacg ctatacaaga agtggttcgg tgattttgtt tcacgattcc gtgaaggcaa
    14161 aaaacaatat gctgacggtt cttccacaag ccataagttt tctgttggca aaaggttatc
    14221 gtttcgaaaa actctgatgt attttttgtt tttctcaata atttattgat tcaaaagtct
    14281 gtatttctta actgaaaatc gcttccatga aagaatctat tttgcaatat gtatggcagc
    14341 ataagctttt tttacaaagt aatctgcaaa ctgctgatgg agaaaaagtg gaaatcattg
    14401 atgtgggcaa aagcaatacg gatgcggggc ccgatttttt taattctaaa ataaaaatcg
    14461 acggaaccct ttgggtgggt aatgtggaaa ttcacctccg ttcttcggaa tggataaaac
    14521 acggacacca gaaagataaa gcttatgata ccgtaatttt gcatgtggtg gcagaaatgg
    14581 atggagaagt cttcagaacg gacggaacaa aaattcctca gctggtttta ccattttcaa
    14641 aagatattga agcgaattac gattcgttgt tgaacaataa taaatggatc ccttgcgcag
    14701 ataaaattga gaaaatttcc gaaattttta ttcaaagttg gaaaaatgct ttgctggtgg
    14761 agcgattgga acaaaaaatg acagagatag aaaatttgtt gacacaaaat cattatcatt
    14821 gggaagaagc tttttacatt ataatagcgc gaaattttgg ttttggcacc aatggtcagg
    14881 ctttcgaact gttggcaaaa tctgttccgc tgaatgtgtt ggccaagcat aaggataatc
    14941 tgttgcaatt ggaagccatt ttgttcggtc aggcaggttt gcttcctgtt gatacatcgg
    15001 atgatgaata cgctatgcaa ctgataaagg aatacgcttt tttgagaaac aaattttcgt
    15061 tacagcccat cgactgcagc caatggaaat tattcagact caggccttat aattttcctc
    15121 atgttagaat tgcacaattt gcttccctga ttcatcagtc atcaaagctt ttctcaaaaa
    15181 ttatcgaaac tcccaatttg gaattgatga aagacctgtt ttcctgcaaa ccttccaatt
    15241 attggcaaac gcattatctg tttggctctg caagtccaaa aagcaccaaa cagctcggat
    15301 tgtattctat ctacagcatt gtaatcaata caattgttcc ctttttgttt tgttatgctt
    15361 caaaaaagaa caacgaagat ttaaaagata aatctttgca tttgctggaa gccattcctt
    15421 cagaaaaaaa tgccattgtc aaaggttgga gaaatattgg aattgcttcc gaatcagcat
    15481 ttgattctca ggctcttata caactgaaaa agcaatattg cgatacgaag aaatgtttgt
    15541 tttgccgaat tggtcacaaa gtattaacgt tgcaaacaac ctgacttgaa acaacaatga
    15601 gattaaattt ttttaagacg atcgcttttt caggaataat tttatcggtt gttggctgtg
    15661 ccaacaaagc acaaggccca accggcggcc caaaagactc cataccgcca agtgttgttc
    15721 gctctgtccc ttccaacggt gcgttaaatt ttaagaaaaa gcaaattctg atcgattttg
    15781 atgaaaatgt aagtatagaa aaagcggttg aaaatgtgct gatttctcct cctcaggtga
    15841 aaccgcccga agttgttgcc atcggaaaga gagttacggt caatttgcag gacgatttaa
    15901 aagacagtac aacctacact attaatttcg ggaatgccat agtagatttg aacgaaaaaa
    15961 atccattaaa aaatttttct ttctcgtttt ctaccggaaa tcagatcgat accttgcaag
    16021 ttaagggaac tgttatcaat gcggaagatt tgaatccggt ttcgggagtt attgttggtg
    16081 tttataatga tttatccgat acggttttta cacaaaaacc ttttttgaga attgggaaaa
    16141 cggatgaaat cgggaagttt tcagtagaaa atttgaaaaa ggatactttc aaaatatatg
    16201 ctttgggcga cgccaacaga gattattttt atcagccggg tgaagggctt gcttttaccg
    16261 attcgctgat tgttcctgga tttttcagag aagaagtgat ggatacgatt tggaaagatt
    16321 ctgttcaaat cgataccatt cgctccattg tacgaacaca ttttttccct gataacatcc
    16381 tgctgaaata ttttaaggaa aacaaaaaaa gacaatattt cattaaagct gaacgaactt
    16441 taccttatgc tttttctttg tttttcagtg cacccatgac tgaattgcca accttgaaac
    16501 caattgattt tgaatgggat ggcaaatata ttcttcagaa aaacaatacg atggacagtc
    16561 tgacttactg gatgactgat tcgttggttt ggaagaaaga cacgttgcag tttgaaatga
    16621 gttatcagaa aaccgattcg ctttttcgac ttgtacctgc aacggataca ttgaaaattt
    16681 tcatgcgaac tgctcgctct actgtgagaa ataccaaaga caaaaaagaa cctgtttcac
    16741 gggtaaaaat ggagcaaaat taccagttta caaccaatat tgccggttct tttgatattt
    16801 atcagcctgt tgtttttcgt ttcagtacgc ctctggctta ttttgattta tccaaaattt
    16861 cacttgctca aagagttgat acgattttaa aacctttgcc tgtaaattgg cgacaggtag
    16921 attctaccaa aatggtttat gctgtcgatt acaaatggga accggagaaa aattatgagt
    16981 tgacagtcga ttcggctgct tttaccagca tatatcagca aaccagtaag aaatattccg
    17041 gaaaattcaa aattcgttct ttggatgaat acagcacggt tattatggtt ttggaaaatt
    17101 ttgttcccaa cgccgtgatt caaattttgg atgccaaaga tgcggtgatt gcttcaaagc
    17161 aggcacagga aaaaggaacc gtttttcagt atctgaaacc cggcgattat tatgctcgac
    17221 tgtttttaga tgagaatgga aacggcaaat gggataccgg agactttgct gctcacaggc
    17281 aaccggaagc ggttttctat tatccgaaaa aaatgactct aatgcccaat tgggaatttg
    17341 aagaaacatg ggattatctt aaagtgcctc tgcttgaaca gaagcccaag gaaattcgca
    17401 aagatgccgg aaagaaggta tcgggaaatt gagatgaaga aaggttgccg aatcattttc
    17461 agaataccac tcatcaaaac aattccatat ttcccatttt gcccataaag agaatgacac
    17521 ccgtactgtt ttcacgaatc acaaataaaa atggtttgtt tactcgaaat tctggaatga
    17581 tgggaacaga agtatatcca acttctacta ccgttacagc agcggcttca gttccttctt
    17641 ccgtaacttc gatataagta ttgtgaagaa ttctcgaaat aaaaagattt tcgttggcaa
    17701 tgtttgtaaa atcggcatta tcggtaaatg ccaatttcat gcccatgtca atcagcgggt
    17761 cgtttaaaag gaatttattt ttactgctga aacgtggaaa ataaacttcc acttctctca
    17821 tagataaatt ttgaagaatg ctgttccact gatctactgt aagatattcc aacaaatctg
    17881 ccgttgtatt tccatttttc ggtaaaatta ccgtcatgct gaaagctttg tttccgtaag
    17941 gcatatcgag gtattgagcc ttttgatctt ctgcataggc aaaagtatct ttctgaaaca
    18001 tcatatttac tttcactttt tcgcctgctt cgttggtaaa atcagtttgc ttagtgtttt
    18061 tcttctcaaa ttgatgacgc cagattccct tgaaataaat ggcattgatc agatacatca
    18121 ctgcttcatc gggaattgca tccagtaccg aaggaatcaa attattggtt tttttagcac
    18181 accattgatt aatggtatcc accgcccaag cttttgaaaa atcgagttct cgtacatacg
    18241 cattaaaata atcgatatta actttcaaaa aatcctgttt tacctgaaaa ccgctgcgat
    18301 accacaaaga attggcaaga gtgagtttgg tggtcgggtc aatggttgga agcgtggttt
    18361 gcaaaatacg ataatattca ttgatttctt caactgacat gccactcatt ttcaaagcac
    18421 tttccatttc ggatttggtt tcgccggctg ctccgttcca cgtcattccc agtgcaatgc
    18481 tgacacttaa gggagaaata aaaacattcg tttcttttga aaaattgatg actttacgaa
    18541 ataaatcgag agcaaattca ttgtcttgcc ctacacgttt tgcttgtccg tcggtcaaag
    18601 taataggttt tgcatcgggc ggaagttgtg aggcagattc agtttcgtcg ttgcatgaaa
    18661 caattccgaa acccagaatg gcaagtagta gaagtttttt catggctaaa atcttttgaa
    18721 atgaaaagaa tataagctac aatttaagga ataatcggca attcatgtta agtttcacaa
    18781 ggttgttgat gaacaacttg gattttcaat aggcttcaaa tgtataa
//
LOCUS       DAOSCH010000028        18656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2066_length_18656_cov_5.722273, whole genome shotgun sequence.
ACCESSION   DAOSCH010000028 DAOSCH010000000
VERSION     DAOSCH010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18656
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2066_length_18656_cov_5.722273"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /gene="rplB"
                     /locus_tag="PLZ54_03595"
     CDS             <1..167
                     /gene="rplB"
                     /locus_tag="PLZ54_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOJ66051.1"
                     /translation="RGVVMNPVDHPMGGGEGRASGGHPRSRKGLLAKGYKTRHPKKQS
                     SKYIIERRKK"
     gene            190..456
                     /gene="rpsS"
                     /locus_tag="PLZ54_03600"
     CDS             190..456
                     /gene="rpsS"
                     /locus_tag="PLZ54_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOJ66052.1"
                     /translation="MSRSLKKGPYISLKLEKKILDMNESGKKTVVKTWARASMISPDF
                     VGHTIAVHNGNKFIPVYITENMVGHKLGEFALTRTFRGHAGKKK"
     gene            500..913
                     /gene="rplV"
                     /locus_tag="PLZ54_03605"
     CDS             500..913
                     /gene="rplV"
                     /locus_tag="PLZ54_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOJ66053.1"
                     /translation="MGARKKISADRRKEARQTLYFAKLNDVPTSPRKMRLVADMIRGM
                     EVNKALSVLKFSSKEASRRMEKLLKSAISNWEIKTEQKADNANLFVSKVYVNEGTTLK
                     RLRTAPQGRGYRIRKRSNHVTLYVDSRNNNDTNQI"
     gene            922..1674
                     /gene="rpsC"
                     /locus_tag="PLZ54_03610"
     CDS             922..1674
                     /gene="rpsC"
                     /locus_tag="PLZ54_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOJ66054.1"
                     /translation="MGQKTNPISNRLGIIRGWDSNWYGGKKYGDTLLEDSKIRKYLNV
                     RLSKASVSRIIIERTLKLVTITVCTARPGIIIGKGGQEVDKLKEELKKITDKEIQINI
                     FEIKRPELDAVIVASNIAHQLEGKIAYRRAVKMAIASTMRMGAEGIKVQVSGRLNGAE
                     MARSEMYKEGRTPLHTFRADIDYACAEALTKVGLIGIKVWICRGEVYGKRDLAPSYVT
                     GQKEGSRTERRSGDHHSGGDHGNKNSSRRKKK"
     gene            1699..2133
                     /gene="rplP"
                     /locus_tag="PLZ54_03615"
     CDS             1699..2133
                     /gene="rplP"
                     /locus_tag="PLZ54_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOJ66055.1"
                     /translation="MLQPKKTKYRRQQKGRMKGNAQRGNQLAFGSFGIKSMDTVWLTG
                     QQIEAARIAVTRYMQRQGQVWIRVFPDKPITKKPAEVRMGKGKGSPEGFVAPITPGRI
                     IIEVDGVPYAVAKEALRLGAQKLPIITKFVVRRDYDLTDQNL"
     gene            2137..2331
                     /gene="rpmC"
                     /locus_tag="PLZ54_03620"
     CDS             2137..2331
                     /gene="rpmC"
                     /locus_tag="PLZ54_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOJ66056.1"
                     /translation="MKTREIRELSTKELLERLETEKENLVRLKLNHSISPLDNPMQIK
                     VVRRNIARIATELRLREINQ"
     gene            2346..2603
                     /gene="rpsQ"
                     /locus_tag="PLZ54_03625"
     CDS             2346..2603
                     /gene="rpsQ"
                     /locus_tag="PLZ54_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOJ66057.1"
                     /translation="METRNLRKERIGVVTSNKMDKTITVAVRWKEKHPIYGKFVNKTK
                     KYYAHDEKNECNIGDTVSIMETRPLSKLKRWRLTEIIERAK"
     gene            2606..2971
                     /gene="rplN"
                     /locus_tag="PLZ54_03630"
     CDS             2606..2971
                     /gene="rplN"
                     /locus_tag="PLZ54_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOJ66058.1"
                     /translation="MIQQETRLLVADNSGAKEALCIRVLGGTGKRYATVGDTIVVSIK
                     SVIPSSDIKKGMVSKAVVVRTKKEVRRVDGSYIRFDDNACVLLNNAGELRGSRIFGPV
                     ARELRATNMKIISLAPEVL"
     gene            2994..3305
                     /gene="rplX"
                     /locus_tag="PLZ54_03635"
     CDS             2994..3305
                     /gene="rplX"
                     /locus_tag="PLZ54_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOJ66059.1"
                     /translation="MTKLHIKKGDIVYVNTGVDKGKTGRVLEVLVKEQRAIVEGINLV
                     SKNMKPNAKYPQGGIVKQEAPIHISNLNPVENGRPVRVGRKLNNEGKLVRISKKTGEE
                     I"
     gene            3308..3853
                     /gene="rplE"
                     /locus_tag="PLZ54_03640"
     CDS             3308..3853
                     /gene="rplE"
                     /locus_tag="PLZ54_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOJ66060.1"
                     /translation="MNTASLKQEYKERIVPALMKEFAYKSVMQVPRLEKIVLNQGLGI
                     AVADRKIIDTAINEMTTITGQKAVATVSKKDISNFKVRKKMPIGVRVTLRGDRMYEFL
                     ERLIRVALPRIRDFKGIENKLDGRGNYTLGITEQIIFPEINIDSISRLLGMNITFVTT
                     AKTDEEGFALLKEFGLPFKKN"
     gene            3867..4136
                     /gene="rpsN"
                     /locus_tag="PLZ54_03645"
     CDS             3867..4136
                     /gene="rpsN"
                     /locus_tag="PLZ54_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOJ66061.1"
                     /translation="MAKESMKAREVKRNKMVAKYEAKRSLLLAEGNYEALQSIPKNAS
                     PVRLHNRCQLTGRPKGYIRQFGVSRIVFREMASKGLIPGVKKASW"
     gene            4257..4652
                     /gene="rpsH"
                     /locus_tag="PLZ54_03650"
     CDS             4257..4652
                     /gene="rpsH"
                     /locus_tag="PLZ54_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOJ66062.1"
                     /translation="MTDPIADYLTRLRNAIKANHRVVEIPASNLKREITRILFEQGYI
                     LNYKVVEDGPQGTIKIALKYDPVNKVNSIKKLIRVSKPGLRKYVGYQDIPRVLNGLGI
                     AILSTSSGVMTNKEAAKLKIGGEVLCYVY"
     gene            4671..5222
                     /gene="rplF"
                     /locus_tag="PLZ54_03655"
     CDS             4671..5222
                     /gene="rplF"
                     /locus_tag="PLZ54_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOJ66063.1"
                     /translation="MSRIGKLPIKIPAGVNVTIKNNLVSVSGPKGTLSQQIHPNISVE
                     VKDGFCTLTRIDDEKENRAMHGLYRSLINNMVEGVSNGFKKTMELVGVGYRVSAQGQV
                     LEFALGYSHSIFLKVPPEVKVETKSERNQNPLIILESCDKQLLGQVCAKIRSFRKPEP
                     YKGKGIKFQGEVLRRKAGKSAGK"
     gene            5244..5585
                     /gene="rplR"
                     /locus_tag="PLZ54_03660"
     CDS             5244..5585
                     /gene="rplR"
                     /locus_tag="PLZ54_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOJ66064.1"
                     /translation="MTKLDRRQKIKAHIRKKIFGTAQRPRMTVFRSNAQIYVQLIDDE
                     KGITLASASSLAIKEKITKQEQAGKVGALIAQAAKEAGITEVVFDRNGYLYHGRVKQL
                     ADAAREGGLKF"
     gene            5592..6110
                     /gene="rpsE"
                     /locus_tag="PLZ54_03665"
     CDS             5592..6110
                     /gene="rpsE"
                     /locus_tag="PLZ54_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOJ66065.1"
                     /translation="MATMMNRVKTTTDLELKDRLVAVNRVTKVTKGGRTFSFSAIVVV
                     GNEDGIVGWGLGKASEVTAAIAKGTEVAKKNLIKVPVLNGTIPHEQEAKFGGARVFIK
                     PASSGTGVTAGGAMRAVLESVGITDVLAKSKGSSNPHNLVKATIAALSELRDAYTVAQ
                     NRKVSLDKVFNG"
     gene            6123..6299
                     /gene="rpmD"
                     /locus_tag="PLZ54_03670"
     CDS             6123..6299
                     /gene="rpmD"
                     /locus_tag="PLZ54_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOJ66066.1"
                     /translation="MATIKIKQVKSKIKSPKVQKLTLEALGLHKINDVVEHEATPQIL
                     GMVEKVKHLVKIVD"
     gene            6329..6778
                     /gene="rplO"
                     /locus_tag="PLZ54_03675"
     CDS             6329..6778
                     /gene="rplO"
                     /locus_tag="PLZ54_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOJ66067.1"
                     /translation="MDLHNLTPAAGSVKSNKRKGRGSGSGMGGTSTRGHKGAKSRSGY
                     AHKIGFEGGQMPIQRRLPKFGFKNINKIEYKAINIDTLEILAQENQLSKIGIDDLRQA
                     GFISKNQLVKILGRGKLTVKLDVEAHAFSKSAENAIVGAGGTAVKIS"
     gene            6780..8123
                     /gene="secY"
                     /locus_tag="PLZ54_03680"
     CDS             6780..8123
                     /gene="secY"
                     /locus_tag="PLZ54_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOJ66068.1"
                     /translation="MNKLIETLKNIWKIEDLRNRLLTTLLFVMIYRFGSYVVLPGIDA
                     GALTALKAQTSGGLLALLDMFSGGAFANASIFALGIMPYISASIVIQLLTIAVPYFQK
                     MQREGESGRRKMNQITRYLTVGILLLQGPSYLINLSVQLRQAGSALPSGFFFQATSTI
                     ILCAGSMFVMWLGERITDRGIGNGISFIILVGIIARLPQAIVREFASRLTDVGGGLIM
                     FIIEILFLLLVIGASIMLVQGTRKVPVQYAKRVVGNKQYGGVRQYIPLKINAAGVMPI
                     IFAQAIMFIPITIVGFSKSESMQGFVGAFMDNTGFWYNFVFAILIILFTYFYTAITIN
                     PTQMAEEMKRNNGFIPGVKPGKKTAEYLDMIMSRITLPGSIFLAIIAIMPAFASIFGV
                     NHAFAQFFGGTSLLILVGVTLDTLQQIESHLLMRHYDGLLKSGRIKGRTSGVSAY"
     gene            8141..8926
                     /gene="map"
                     /locus_tag="PLZ54_03685"
     CDS             8141..8926
                     /gene="map"
                     /locus_tag="PLZ54_03685"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOJ66069.1"
                     /translation="MIYLKSEEEIELLRISNQLVAKTLAELAKHITPGISTLELDRIA
                     DEFIRDHGAVPSFLDYNGYPNSICTSVNEQIVHGIPSDKVFLKEGDIISIDCGAYINC
                     FHGDSAYTFCVGEVDQQIVNLLKTTKESLYKGIEQAQEGHRTGDIGYAVQSYCEQRGY
                     SVVREMVGHGLGRGIHEAPEVPNYGRKGNGVMLKYGMVICIEPMINMGTRHIVFERDG
                     WTTRTLDRKPSAHFEHAVAIRQGKADILSSFEYIEQVLGERAI"
     gene            8932..9150
                     /gene="infA"
                     /locus_tag="PLZ54_03690"
     CDS             8932..9150
                     /gene="infA"
                     /locus_tag="PLZ54_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOJ66070.1"
                     /translation="MAKQTAIEQDGTIIEALSNAMFRVELENGHIITAHISGKMRMNY
                     IKILPGDKVKVEISPYDLTKGRITFRYK"
     gene            9167..9283
                     /gene="ykgO"
                     /locus_tag="PLZ54_03695"
     CDS             9167..9283
                     /gene="ykgO"
                     /locus_tag="PLZ54_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HOJ66071.1"
                     /translation="MKVRASIKKRSEDCKIVRRKGRLYVINKKNPRFKQRQG"
     gene            9310..9690
                     /gene="rpsM"
                     /locus_tag="PLZ54_03700"
     CDS             9310..9690
                     /gene="rpsM"
                     /locus_tag="PLZ54_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOJ66072.1"
                     /translation="MAIRIVGVDLPQNKRGEIALTYIFGIGRSSANKILSKAGVDRDI
                     KVKDWTDEQAAKIREIISSEYKVEGDLRSEVQLNIKRLMDIGCYRGIRHRLGLPVRGQ
                     STKNNARTRKGKKKTVANKKKATK"
     gene            9739..10128
                     /gene="rpsK"
                     /locus_tag="PLZ54_03705"
     CDS             9739..10128
                     /gene="rpsK"
                     /locus_tag="PLZ54_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOJ66073.1"
                     /translation="MAKKTVAAKKRVVKVDAAGQLHVHSSFNNIIISLTNENGEVISW
                     SSAGKMGFRGSKKNTPYAAQMAAQDCAKIAFDLGLRKVKAYVKGPGNGRESAIRTVNG
                     IGIEITEIVDVTPLPHNGCRPPKRRRV"
     gene            10291..10896
                     /gene="rpsD"
                     /locus_tag="PLZ54_03710"
     CDS             10291..10896
                     /gene="rpsD"
                     /locus_tag="PLZ54_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOJ66074.1"
                     /translation="MARYIGPKTKIARKFGEAIFGPDKVLSKKNYPPGQHGNNRRKKT
                     SEYGIQLREKQKAKYTYGVLEKQFHIIYERARRASGVTGVILLQMLESRLDNLVFRMG
                     IAPTRAAARQLVNHKHITVDGKVVNIPSYQVKPGQVVGVREKDKSMEVIQNSLSGFNH
                     SKYPWIEWNASTLSGTYLHIPEREDIPENIKEHLIVELYSK"
     gene            10908..11900
                     /locus_tag="PLZ54_03715"
     CDS             10908..11900
                     /locus_tag="PLZ54_03715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOJ66075.1"
                     /translation="MSILAFQKPEKVIMLEADDFHGKFEFRPLEPGYGITIGNALRRI
                     LLSSLEGFAITSIKIEGIDHEYSTIPGVIDDVTNIILNLKKVRFKQVVEGVDNEKVSI
                     TVSDTAVFKAGDIGKHLTGFEVLNPELIICELDPKGSFQIDIAINKGRGYVPAEENRT
                     PNLEIGVIPIDSIFTPIRNVKYAIENYRVEQVTDYEKLVIEILTDGSIPPKEALKEAA
                     KILIQHFMLFSDEKIEVETVEGETTSEFDEEVLHMRQLLKTKLTDLELSVRALNCLKA
                     ADIETLGQLCSFQRNDLLKIRNFGKKSLAELEEKLISLNLSFGMNVSKYKLDKD"
     gene            11906..12436
                     /gene="rplQ"
                     /locus_tag="PLZ54_03720"
     CDS             11906..12436
                     /gene="rplQ"
                     /locus_tag="PLZ54_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOJ66076.1"
                     /translation="MRHNKKFNHLSRTSSHRKALLSNMASSLILHKRISTTLPKAKAL
                     RVYVEPLLTRAKEDTTQSRRLVFSYLQNKEAVTELFQNISVKIANRPGGYTRILRTGF
                     RLGDNAEMCIIELVDYNENMLKDKTTKKITRTRRSSSRKKAETEVQAVPATSEVSAPK
                     PVEQAPAENQAPTTEE"
     gene            complement(12585..13586)
                     /gene="gap"
                     /locus_tag="PLZ54_03725"
     CDS             complement(12585..13586)
                     /gene="gap"
                     /locus_tag="PLZ54_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOJ66077.1"
                     /translation="MIKVGINGFGRIGRLVFRAAQERNDIEVVAVNDPFLDKDYLIYM
                     LKFDSVHGKFQGTAENKDGKLIINGKEVLFFAEKDPANIGWGAAGAEYVVESTGVFTT
                     VEKASAHLKAGAKKVVISAPSNDAPMFVCGVNLDAYTKDMDIVSNASCTTNCLAPLAK
                     VIHDNFGIVEGLMTTIHAATNTQKTVDAPSNKDWRGGRSILNNIIPSSTGAAKAVGKV
                     IPSLNGKLTGMSMRVPTADVSVVDLTVRLEKAATYEEIKAAVKAASENEMKGILGYTE
                     DAVVSTDFIHDPRTSIFDANAGIALNSNFVKLVSWYDNEWGYSNKVLDLICHMDSVK"
     gene            complement(13814..14068)
                     /locus_tag="PLZ54_03730"
     CDS             complement(13814..14068)
                     /locus_tag="PLZ54_03730"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOJ66078.1"
                     /translation="MKTNVGSTDKVVRLALAIVLIVLFYKGIFSVILGIVALIVAFVL
                     TLTSLIGFCPLYTLFGINTAKKKEQSENAEQPTKKRQPKN"
     gene            14382..15182
                     /gene="proC"
                     /locus_tag="PLZ54_03735"
     CDS             14382..15182
                     /gene="proC"
                     /locus_tag="PLZ54_03735"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOJ66079.1"
                     /translation="MEIKNLKIAIIGAGNMGGALARGWSKSDILSAENIAISDISQEK
                     LDQLTAFNPHFTVSTSNREIIRQADIVLLAIKPWLVETVAKEISKSIDYKKQVIISIA
                     AGVSFETLTHFFDSDGVFFRVIPNVAVEIGQGVSVVSSFNACKEHQQLLLEIFNVLGK
                     TFFVSENQMDAFMALTSCGIAYAFRYVRASVAGAVEMGISPQMAQEAVIQTVRGAVDL
                     LELHQSHPETEIDKVTTPGGFTIKGLNEMEANGFSNAVIKGLKASYSK"
     gene            15228..15776
                     /locus_tag="PLZ54_03740"
     CDS             15228..15776
                     /locus_tag="PLZ54_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOJ66080.1"
                     /translation="MNIQIKQIAERLRALRESLELTPEQAAEKCGATTEEYQQYESGD
                     YDIPMSFICQAADSFGVATTALLVGDDPHAQVFFVTRKGTGASVERTKAYKYQALAYG
                     FRHPKAEPFEVTVEPNDNPLTLNTHFNQEFNYVLEGTLLLRIAGNDLILNEGDSIYFD
                     ATRPHGMKALNGKRVKFLAVII"
     gene            15882..17537
                     /locus_tag="PLZ54_03745"
     CDS             15882..17537
                     /locus_tag="PLZ54_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOJ66081.1"
                     /translation="MLEKYLKQTEFKDFDDFKANYQLIVPEHFNFAYDVIDEWAAKEP
                     DKLAMIWTNDQGECRKFSFAEMKKYSDQTASYFQSLGIGKGDVVMAILKRRYEFWFTI
                     LALHKLGAITIPDTHLLTPKDLIYRNNAADIKAIVAVGEEPIVSHINDSMAESPTVEM
                     LISIGPIVPENWLDFHKGIDEATPFTPIHHVNENTDPLIISFTSGTTGNPKMVLLDCL
                     YPLGHIITAKYWHNLHENSIHLTIADTGWLKALWGKIYGQWIVGACVFVYDHEKFTPA
                     NMLEMIEKYHVTSLCAPPTIFRFLIREDISKYDLSSLEYATTAGEALNASVYEKLYEL
                     TGIKLHEGYGQSETTVCVYTTPWIEPKPGSMGLPGPHYDVDIQMPDGRSAEVGEQGEI
                     VIKLHKNYPVGLFKGYYRNEELTREAFRGNVYHTGDMAWKDEEGYFWFVGRTDDVIKS
                     SGYRIGPFEVESALMTHPAVVECAITGVPDELRGQVVKATVILAKEYKDKAGEELVKE
                     LQNHVKKVTAPYKYPRIIEFVDELPKTISGKIRRVEIREKDKQ"
     gene            17599..18252
                     /gene="upp"
                     /locus_tag="PLZ54_03750"
     CDS             17599..18252
                     /gene="upp"
                     /locus_tag="PLZ54_03750"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOJ66082.1"
                     /translation="MKIINLSDNNSILNQFIKEIRSVEIQKDSMRFRRNMERIGEIMA
                     YEISKAMDYVEEKVQTPLGIAPVKVIDEKVVISTILRAGLPFHQGFLNYFDGSENAFV
                     SAYRKYRDAENFEIFVEYIASPGLEGKTLILTDPMLATGSSMEQTFSALTTKGFPSKI
                     HIATVIASRHAINYLTTHFPENITTIWAAAIDPEINSHSYIIPGLGDAGDLAFGEKL"
     gene            complement(18308..18562)
                     /gene="rpsT"
                     /locus_tag="PLZ54_03755"
     CDS             complement(18308..18562)
                     /gene="rpsT"
                     /locus_tag="PLZ54_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOJ66083.1"
                     /translation="MANNNSAIKRIRQANKKRLHNRYYIKSTRTAIKRLRTTTDKEKA
                     TELLPQVSSMLDKLAKRNMIHKNKANNLKSKLAIHVNKLA"
BASE COUNT         6135 a         3227 c         3990 g         5304 t
ORIGIN      
        1 tgagaggtgt tgtaatgaat cctgtagatc acccaatggg tggtggagaa ggccgtgctt
       61 ccggtggtca tcctcgttcg cgcaaaggac ttttggctaa agggtacaaa acacgtcatc
      121 ccaaaaagca gtccagcaag tacataattg aaagaaggaa aaaataattc aataatgaag
      181 taaatcaata tgagccgttc attaaaaaaa ggaccttata tcagtctgaa acttgaaaag
      241 aaaatacttg acatgaatga aagcggaaag aaaaccgttg tcaagacatg ggcacgtgct
      301 tccatgattt cgcctgattt tgtgggtcat actattgctg ttcacaatgg aaacaaattt
      361 attccggtgt atatcaccga aaacatggtt ggacacaaac tgggtgaatt tgcactgacg
      421 cgtactttcc gtggacatgc aggaaagaaa aaataaacca ttgaagcagt aagttagaaa
      481 attaaaaaaa ttggtaaaaa tgggtgctag aaaaaaaata tctgctgata gaagaaaaga
      541 agctcgtcaa acgctctatt tcgctaagtt gaatgacgtt cctacttctc cgcgcaaaat
      601 gaggcttgtt gccgacatga tcagaggaat ggaagtaaat aaggctttaa gcgtgttgaa
      661 attttcatca aaggaagctt ctcgcagaat ggagaaatta cttaaatcgg ccatcagcaa
      721 ttgggaaatc aagaccgaac aaaaagccga taatgctaat ttgtttgtaa gcaaagtata
      781 tgtgaatgaa ggaactacgt taaaaagatt acgtacggcc ccgcaaggac gcggttatcg
      841 tattcgcaaa cgttccaatc acgttacttt atatgtggat tcaagaaata ataacgatac
      901 aaatcaaatt taaatagctc aatgggacaa aaaactaatc caataagcaa tcgcttggga
      961 attatacgtg gttgggattc aaattggtac ggtgggaaaa aatatggcga caccttgctc
     1021 gaagacagta agataagaaa atatttaaat gtccgcctat ccaaagcaag tgtatcccga
     1081 atcattatcg aacgaaccct taagcttgta accataacag tttgcacggc tcgtcccggt
     1141 atcattatcg gtaaaggagg acaggaagta gataagttaa aggaagaatt gaagaaaatt
     1201 accgataagg aaattcaaat caatattttc gaaatcaagc gcccagaact tgatgcagtg
     1261 atagttgcca gtaacattgc tcatcagctg gaaggaaaaa ttgcataccg aagagccgta
     1321 aaaatggcta ttgcttctac catgcgaatg ggagcagaag gaatcaaagt tcaggtttcc
     1381 ggtcgtttga acggtgctga aatggcacgt tcggaaatgt acaaggaagg cagaacaccg
     1441 ttgcatactt tcagagccga tattgattat gcttgcgccg aagctttgac aaaagtaggt
     1501 ttgataggca taaaagtgtg gatttgcaga ggcgaagttt acggtaagcg cgatttggct
     1561 ccttcctatg ttacaggaca aaaagaaggc tcacgtaccg aacgtcgttc gggagaccac
     1621 cactcgggag gcgatcatgg aaataaaaat tcttccagaa gaaagaaaaa ataaaaatat
     1681 aatttgatat aaggcatcat gttacaacct aaaaaaacaa aatacagaag acagcagaaa
     1741 gggcgcatga aaggaaatgc gcagcgcggg aatcaactgg ctttcggatc atttggtatc
     1801 aaatctatgg atacagtttg gttgaccgga caacaaatag aagctgcacg tattgctgta
     1861 actcgttata tgcaacgtca gggtcaggtt tggattcgtg tttttcccga taaacctata
     1921 accaaaaaac ctgcagaagt acgtatggga aaaggtaaag gttctcccga aggttttgtt
     1981 gctccaataa cgcctggcag gataattata gaagtggatg gagtacccta tgccgttgca
     2041 aaagaagcat tacgtttagg tgctcaaaaa ttgcccatta taacaaaatt tgtagtaaga
     2101 agagattatg atctcacaga tcaaaatttg taaaaaatga aaacgcgaga aatcagagaa
     2161 cttagcacca aagaactatt ggaacgatta gaaactgaaa aagaaaattt agttcgttta
     2221 aaactaaatc attcgatcag tccattggac aatccaatgc aaataaaagt tgttcgtaga
     2281 aatattgcac gtattgctac agaattgcgt ctgcgagaaa ttaatcaata aattaagtac
     2341 acccgatgga aacaagaaat ttaagaaaag aaagaattgg tgtagttacc agcaacaaaa
     2401 tggataaaac cataactgta gctgtaaggt ggaaagaaaa acaccctatt tacggtaaat
     2461 ttgtaaataa aaccaaaaag tattatgctc acgacgaaaa aaacgaatgc aatataggtg
     2521 ataccgtcag cattatggaa actcgtccgc tgagtaagtt aaaaagatgg agattgactg
     2581 aaataataga aagagccaaa taattatgat acagcaagaa acaagacttc tcgttgccga
     2641 caatagcgga gccaaagagg ctttgtgcat ccgagtttta ggcggaaccg gcaagcgata
     2701 tgcaacagta ggggatacca ttgtcgtttc aataaaaagt gtaatacctt catcagacat
     2761 taaaaaagga atggtatcaa aagcagtagt agttcgaaca aaaaaagaag ttcgacgtgt
     2821 tgatggttca tatatacgct ttgatgacaa tgcgtgtgta ttgttaaaca atgctggtga
     2881 acttcgtggg agtcgtattt ttggaccggt agctcgtgag ttgcgtgcca ccaatatgaa
     2941 aatcatttct ttggcaccgg aagtgcttta acataagaaa aatttatata gagatgacga
     3001 agttacatat taaaaaaggc gatatagttt acgtaaatac gggagtagac aaaggtaaaa
     3061 ccggacgcgt attggaagtg cttgttaagg aacaacgtgc tattgtcgaa ggcattaatt
     3121 tggtgtcaaa aaacatgaaa cccaatgcca aatatccaca aggtggtatt gttaaacaag
     3181 aagcgcccat tcatatttcc aatttaaatc cggtagaaaa tggtcgaccg gtgcgtgttg
     3241 gtcgaaaact gaataatgaa ggtaaattag ttcgaatttc aaaaaagaca ggagaggaaa
     3301 tttaagaatg aatacagcaa gtttaaaaca ggaatacaaa gaacgaattg ttccggcttt
     3361 aatgaaagaa tttgcttaca aatcggtgat gcaggttccc aggttggaaa aaattgttct
     3421 taatcaaggg ttgggtattg ccgttgccga caggaagatt attgataccg caatcaatga
     3481 gatgacgacc attactggac aaaaagccgt agcaaccgtt tccaaaaaag atatttcaaa
     3541 tttcaaggtt cgaaaaaaaa tgcctattgg cgttcgagta accttacgtg gggacagaat
     3601 gtacgagttt ttggaacgtc tgattcgagt tgcccttccc cgtattcgtg actttaaggg
     3661 aatagaaaac aaactcgatg gtcgtggaaa ttacacattg ggaattacgg agcaaattat
     3721 cttccctgaa atcaatatcg attccattag tcgattactg ggaatgaaca taacttttgt
     3781 tactaccgca aaaactgatg aagaaggatt tgctctttta aaagagtttg gtttaccatt
     3841 caaaaaaaat taaaaaagga ataaaaatgg caaaagaatc aatgaaagcc cgagaggtaa
     3901 agagaaacaa aatggtggca aaatacgagg ccaagcgaag tctgttgctt gccgaaggaa
     3961 attatgaagc gcttcaatcc atacccaaga atgcttctcc cgtacgtttg cacaacagat
     4021 gtcagcttac cggacgtcca aaaggatata tccgtcagtt tggtgtatct cgtattgtat
     4081 ttcgtgaaat ggcttcaaaa ggacttattc ccggcgtaaa gaaggcgagc tggtaagttt
     4141 cccttcaaaa atatgctgga ttagtgattt ttcggtttat aaaaattaca tttagaattt
     4201 taccatatat taaatattgt ctcggcagtc gagattaatt taaagtttaa acatttatga
     4261 cagatccaat agcagattat ttaactcggt tgcgaaatgc aattaaagca aaccatcggg
     4321 ttgtagaaat tcctgcttca aatttaaagc gtgaaattac gcgtatttta tttgaacaag
     4381 gctatatcct gaattacaag gttgttgaag atggtccaca gggaacaatc aaaattgcgt
     4441 tgaaatatga ccctgttaac aaagtaaatt caataaagaa attaatcaga gtatctaaac
     4501 ccggtcttcg gaaatatgtt ggataccagg atatacctcg cgtgttaaat ggattgggta
     4561 tagctatcct ttcaacatcc agtggcgtta tgaccaataa ggaagcagcc aaattgaaaa
     4621 ttggcggcga agttttatgt tatgtttatt aagaggcgga ggaaaatgat atgtcaagaa
     4681 taggaaaatt acccataaaa attccggctg gagtcaacgt tacgataaaa aacaatttgg
     4741 tttcagtaag cgggcctaag ggaactttaa gtcagcaaat tcatccaaat atttcagttg
     4801 aagtaaaaga tggtttttgt actttaaccc gcatcgatga tgagaaagaa aatcgtgcca
     4861 tgcacgggct gtatcgttct ttgatcaaca atatggttga aggagtgtct aatggcttta
     4921 aaaagactat ggagttggta ggtgtaggct atcgtgtttc tgctcagggt caggttttgg
     4981 aatttgcttt aggatattcg cacagtattt ttttaaaagt accgcccgaa gttaaggtag
     5041 aaacaaagtc ggaacgtaat cagaatccgc tgattatttt ggaaagttgc gataagcagc
     5101 ttttgggaca ggtttgtgca aaaatacgtt ctttccgtaa acctgaaccg tataaaggaa
     5161 aaggaattaa attccagggt gaagtactgc gaagaaaagc aggaaaatca gccggtaaat
     5221 aatatacgta aatttgtgaa gttatgacaa aattagacag aagacaaaaa ataaaggctc
     5281 atatacggaa aaaaattttt ggaactgctc agcgaccccg aatgaccgta tttagaagca
     5341 acgctcaaat ttatgttcag ctaattgatg atgaaaaggg aataacattg gcttcagctt
     5401 cgtcattagc tattaaggaa aaaataacca agcaagagca agccggtaag gtgggtgctc
     5461 taattgctca ggctgctaag gaagccggta tcactgaagt agtgttcgac cgcaatggtt
     5521 atttatatca cggtagagtt aaacaattgg ccgatgctgc tcgtgaaggc ggtcttaaat
     5581 tttaagaatg tatggcaaca atgatgaata gagtaaaaac aacaacggat ttagaattaa
     5641 aagacagatt agtagctgtc aatcgggtaa ccaaagttac caaaggagga cgaactttta
     5701 gcttctcagc tattgtggtg gtaggaaatg aagatgggat cgtaggctgg ggacttggaa
     5761 aagcaagcga agttaccgca gcaattgcta aaggaacaga agtagccaag aaaaatctaa
     5821 tcaaagtgcc ggtgctgaat gggactattc ctcacgaaca ggaagcaaaa tttggtggtg
     5881 cgcgcgtttt tatcaaacca gcttcttcag gtaccggagt aactgcaggc ggtgcaatgc
     5941 gtgcagtgct cgaaagtgta ggtattacgg atgtgttagc aaaatcaaag ggttcatcca
     6001 atcctcacaa cctggtgaaa gccacgattg ctgccctctc cgaactccgc gatgcataca
     6061 ctgtcgctca gaacagaaag gtttctttgg ataaggtttt taatggataa tttcagataa
     6121 tcatggcaac tattaaaatt aaacaagtta aaagtaaaat aaaatctcct aaagttcaaa
     6181 aattgacttt agaagcactt ggattacata aaatcaatga tgttgttgaa catgaagcta
     6241 ctccacaaat tttgggaatg gttgagaaag ttaaacattt ggtaaagata gtcgattaaa
     6301 aaattttaat ctttaataaa aaggagatat ggatttacat aatttaaccc cagctgcagg
     6361 ctcggtaaaa tcaaataaaa gaaaaggtag aggttctgga tctggaatgg ggggcacatc
     6421 tacacgtgga cataaagggg caaaatctcg ctcggggtat gcgcacaaaa tcggttttga
     6481 aggtgggcag atgccaattc aaagacgctt acccaagttt ggttttaaga atataaataa
     6541 aattgaatat aaagcaatca atatcgatac tttagaaatt ttagcacaag aaaatcaatt
     6601 atctaaaatt gggatcgatg atcttcgtca agccggattt atatctaaaa atcagttggt
     6661 aaaaatctta ggacgcggga aattgaccgt aaaattagat gttgaagctc acgctttttc
     6721 aaaatctgcc gaaaatgcaa ttgttggagc cggtggaacg gcagtaaaaa tttcataaga
     6781 tgaataaact cattgagaca cttaaaaata tttggaagat agaggacttg cgcaacaggt
     6841 tattgacaac attattgttt gtcatgattt atcgctttgg ttcttatgtt gtgcttccgg
     6901 gtattgatgc aggagcatta actgctctta aagctcaaac aagcggagga ctacttgctt
     6961 tattggatat gttttcaggt ggagcgtttg ccaatgcatc catttttgcg ttgggaatta
     7021 tgccgtatat ttcggcttct atcgttattc agttgctgac tatagcagta ccatattttc
     7081 aaaaaatgca acgtgaaggt gaaagtggca gaagaaaaat gaatcagata acgcgttatc
     7141 ttacagtagg aattcttctc ttgcaaggac cttcttattt aattaattta agtgttcagt
     7201 taaggcaggc tggttccgca cttccatcag gttttttctt tcaggcaact tctaccatta
     7261 ttctctgtgc aggtagtatg tttgttatgt ggcttggtga acgaattaca gacagaggaa
     7321 ttggaaacgg aatatcgttt attattttag tcggtattat tgcccgtctg ccgcaagcta
     7381 ttgtaaggga atttgcctct cgattgaccg atgtgggcgg aggtctgatc atgtttatta
     7441 tagaaattct tttcctttta ctggttatcg gagcttctat aatgctggtt cagggaacac
     7501 gtaaagttcc tgttcaatat gcaaaacgtg tggtaggaaa taaacagtat ggtggcgtaa
     7561 ggcagtatat cccgctgaaa attaatgcag ccggagttat gccgataatt tttgctcaag
     7621 ctatcatgtt tattcccatt acaattgttg gattttcaaa atccgagtca atgcagggat
     7681 ttgttggtgc ttttatggat aatacaggtt tctggtacaa ctttgttttt gccatcctga
     7741 ttattttgtt tacgtatttc tatacggcta tcactattaa tccaacgcag atggcagaag
     7801 agatgaaacg taataacgga tttattccag gtgtaaaacc tgggaaaaag acggctgaat
     7861 atcttgatat gattatgtcc agaattacat tgcccggttc aatatttctg gctatcattg
     7921 ccattatgcc tgcgtttgcc agtattttcg gtgtaaacca tgcctttgca caattttttg
     7981 gaggcacctc cctgttaatt ttagttggtg ttacgttgga tactttgcaa caaattgaaa
     8041 gtcatttgtt gatgcgacat tacgacggat tattgaagtc aggacgtatc aaaggacgaa
     8101 caagtggagt ttctgcttat taaataagat aacattaaaa atgatttatc tcaaatcgga
     8161 agaagagata gaattactta gaatcagtaa tcaattggta gctaaaacgt tagctgaact
     8221 cgcaaaacat attacacccg gaatcagtac gttggaactt gatcgaatag ccgatgaatt
     8281 tattcgtgat catggtgcag tgccgagttt tcttgattat aacggatatc ctaattccat
     8341 ctgtacttcg gtaaacgaac agatagtaca tggaattcct tctgataaag tttttttaaa
     8401 ggaaggcgat ataatttcga ttgactgtgg tgcttatatc aactgttttc atggcgattc
     8461 tgcttatacc ttctgtgttg gagaagtaga tcaacagatt gtcaatctgc tgaaaacaac
     8521 caaagaatca ctttataaag gtattgagca ggctcaggaa ggacatcgta ctggtgatat
     8581 tggttatgct gtacagagtt attgtgaaca acgcggctat tccgtagtgc gtgaaatggt
     8641 tggtcatggt ctgggtagag ggatacacga agcacctgag gtaccgaatt atggaagaaa
     8701 aggcaacggc gtaatgctga aatacggaat ggtgatttgc atagagccca tgattaatat
     8761 gggaacacga catattgttt ttgaaagaga tggatggact acccgaacgc tggacagaaa
     8821 gccttcggca cattttgaac acgccgtagc cattcgtcag ggaaaagcag atatactatc
     8881 aagttttgaa tacattgaac aagttttagg agaaagagca atttaatttt tatggccaaa
     8941 caaacagcaa tagaacagga tggtacgata atagaagcat tatcgaatgc catgtttcgt
     9001 gttgaacttg aaaacggtca cattattaca gcccatattt ccggcaaaat gaggatgaat
     9061 tacataaaaa ttttaccggg agataaggtg aaagttgaaa tatcgcctta tgatttgacc
     9121 aaaggaagaa ttacatttag atacaaataa aaaaatacta tcagagatga aagtgagagc
     9181 atccataaaa aaacgttctg aagattgtaa aatcgttcgc cgaaaaggtc gtctttacgt
     9241 gattaataaa aagaatcccc gcttcaaaca acgtcagggt taattaattt acatgaaaaa
     9301 aattttaata tggcaataag aattgttgga gtagacttac ctcaaaacaa gcgaggtgaa
     9361 attgcgttga cttatatttt cggaattggt cgcagcagcg caaataaaat tttaagtaag
     9421 gccggtgttg atagagacat aaaagtgaaa gactggacag atgaacaagc cgcaaaaatc
     9481 agggagataa tttcttccga atacaaggta gaaggagatt tgcgctcaga agttcagttg
     9541 aacattaaac gattgatgga tataggttgc taccgcggca tccgtcatcg tctcggtttg
     9601 cctgttagag gtcaaagcac caagaacaat gcccgcactc gaaaagggaa gaagaaaact
     9661 gttgcaaaca agaaaaaagc aaccaaataa cagataactg gaaattataa tcatcagaaa
     9721 aagaagatat aaataattat ggcaaagaaa acagttgcag ccaaaaaaag agtagtgaaa
     9781 gtggatgccg caggtcaatt gcacgttcat tcatctttca acaacattat tatttcactt
     9841 accaacgaaa atggtgaagt tatttcttgg tcctctgccg gaaaaatggg attcagaggt
     9901 tcaaagaaaa acactcccta tgctgcgcaa atggcagctc aggattgtgc aaaaatagct
     9961 ttcgacttgg gtttacgtaa ggtaaaagcc tatgtcaaag ggccgggaaa tggacgcgaa
    10021 tcagctatcc gaaccgttaa cggaataggt atcgaaataa ctgagattgt tgatgttaca
    10081 cctcttccac ataatggttg tcgtccaccc aaaaggagaa gagtataata tcaattttcc
    10141 actttttgta tgaatcaaaa agggtaatgc cgacaggtaa aaatttaaaa aattcattgc
    10201 aagttttttc ctctctgcaa tggcggctga agcagttttt acaatgtgga tacccgaata
    10261 aattaattat aacaatcaat tttagaaaaa atggcaagat atattggacc taaaacaaaa
    10321 atagcacgta agtttggcga agctattttt ggacccgata aagtattgag caaaaagaac
    10381 tatcctcccg gacagcacgg gaacaaccga agaaagaaaa cttctgaata cggtattcag
    10441 ctgcgcgaaa aacaaaaggc taaatatacc tacggagttc ttgaaaagca gtttcatatc
    10501 atttatgaac gtgcacgtcg tgcttccggc gtaacaggcg taattttgtt gcaaatgctg
    10561 gaatcgcgtc ttgataattt ggttttcaga atgggaattg cacctacacg tgctgctgcc
    10621 cgtcagttgg taaaccacaa acacatcaca gtggatggta aggtagttaa cattccttcc
    10681 tatcaggtta aacctggtca ggtggtgggt gtacgcgaaa aagacaaatc aatggaagtg
    10741 attcagaatt cgttgagcgg tttcaaccac agcaaatatc cgtggattga atggaatgct
    10801 tcaacgcttt caggaactta tctgcatatc cccgaaagag aggatatccc tgaaaatatc
    10861 aaagaacatt taatcgttga gttatactct aaataatttg taaattcatg tctatattag
    10921 catttcaaaa acccgaaaag gttattatgc tggaggcaga cgattttcat ggaaaatttg
    10981 aatttcgccc tctggaaccc ggctatggca ttactatcgg caatgcattg cgccgtattc
    11041 tcttgtcttc tttggaaggt tttgccatca cttctatcaa aatcgaagga atagatcatg
    11101 aatattctac cattccggga gtgattgatg atgtaaccaa tatcattctc aacctgaaaa
    11161 aagtgcgttt caaacaagtg gtagaaggtg ttgataacga aaaagtttcg attaccgttt
    11221 cggatacagc agtttttaaa gccggagata ttggtaaaca tcttaccggt tttgaagttc
    11281 tcaatccgga gcttattatt tgcgaacttg atccgaaagg aagttttcag attgatattg
    11341 ccatcaacaa gggacgtgga tatgtaccgg ctgaagaaaa cagaactccc aatctcgaaa
    11401 ttggcgttat tcccatcgat tcaattttta cacctatccg taacgtgaaa tatgccatcg
    11461 aaaattatcg tgtggaacag gttacagatt acgaaaaatt agtaattgaa attttaaccg
    11521 atggttctat tcctcctaag gaagcattga aagaggcagc aaaaattctg attcagcatt
    11581 tcatgttgtt ctctgatgag aaaatagaag ttgaaacagt agaaggtgaa acaacaagcg
    11641 aatttgacga agaggttctt cacatgcgtc agttgttaaa aaccaaactt accgatttgg
    11701 aactttcagt cagagcattg aattgtttga aagctgccga tattgaaaca ttgggacaac
    11761 tttgcagttt tcaacgcaac gacctgttga agatccgcaa tttcggcaaa aaatcgctcg
    11821 ccgaactgga agaaaaattg attagtttaa atctttcctt cgggatgaat gtttcaaagt
    11881 ataaattaga taaagattaa atgaaatgag acataataag aaattcaacc atttaagtcg
    11941 tacgtcttct cacaggaaag ctttattgtc caatatggct tcatcgttga ttttgcacaa
    12001 aagaatctcc actacacttc ctaaagctaa agctttaaga gtttacgttg aacctctttt
    12061 gaccagagcc aaggaagata cgactcaatc aagacgtttg gttttcagtt atttgcagaa
    12121 caaggaagct gtaaccgaat tgtttcaaaa tatttcggtg aaaattgcaa atcgtccggg
    12181 tggttatacg cgtattctca ggacgggttt ccgcttggga gataatgcag aaatgtgcat
    12241 tattgagctt gttgattaca acgaaaacat gctcaaggat aaaactacca agaaaataac
    12301 tcgtacacgt cgttcttctt caagaaagaa agcagaaact gaagttcagg cagtacctgc
    12361 cacttcggaa gtttcggctc ctaaacctgt tgaacaggca cctgccgaaa atcaggcacc
    12421 aacgacagaa gaataaaatt gaagattttt tgttaatcat attaaaaagg ggaccacgca
    12481 agctgttccc ttttttgttg ggagtaattg aataaaaaac tccctcaaaa aattaatttt
    12541 gagagagttc aaaaaaacta ttcttttttt tctattaaag ctctttattt aacgctgtcc
    12601 atgtggcaga ttaagtcgag aaccttattt gaataacccc attcgttgtc gtaccagctc
    12661 accagtttaa cgaaattgct gttcaatgca ataccggcat tggcatcaaa aatagaagtt
    12721 ctgggatcgt gaatgaaatc ggttgaaacc acagcatctt cggtgtaacc caaaatacct
    12781 ttcatttcgt tttcagaagc agcttttacg gcagctttaa tttcttcgta ggtagcagct
    12841 ttttccaaac gaacggtaag gtcaactacc gaaacatcag cagtcggaac gcgcattgac
    12901 atacccgtga gttttccgtt cagtgaagga atgacttttc caacagcttt tgcagctccc
    12961 gtagaagaag gaataatgtt gttcaaaatg gaacggccac ctctccagtc tttatttgaa
    13021 ggagcatcaa ctgttttttg agtgttggta gcggcatgaa tggttgtcat caacccttca
    13081 acaattccga aattgtcgtg gataactttg gcaagtggag ccaagcagtt ggtagtacat
    13141 gaagcattgg aaacgatatc catatctttg gtgtatgcat caagattcac accgcacaca
    13201 aacatgggag catcattcga tggagcagaa ataacaactt tctttgcacc ggctttcagg
    13261 tgagcagaag ctttttcaac agtggtgaat acaccggttg attctactac atattcagca
    13321 ccggcagctc cccagccaat gtttgccgga tctttttctg caaagaaaag aacttctttt
    13381 ccgttgataa ttaattttcc atccttattt tcggctgttc cctgaaattt gccatgtacg
    13441 ctgtcaaatt tcaacatgta gatcagataa tccttatcca aaaaaggatc gttcacagca
    13501 acgacttcaa tatcgtttct ttcctgggct gcacggaaaa ccaaacgtcc aatgcggcca
    13561 aaaccattaa tacctacttt aatcatttta tttaagttta gagattagtt acttaaaaaa
    13621 ccgtacaaat ttacgaattt ttgtccgatt ttcaaaggaa caggttgttt aattaaagaa
    13681 atttttaagt aattggaaga tttttttctc cctttccctt ttacatgcat ttgtatcctt
    13741 tgtcgaatga aatgcatgaa gataactcaa tttcgtgtac cgaaaattcc gattcctgac
    13801 gtcatcgatg tcctcagttc ttgggttgtc gttttttagt gggttgttca gcgttttcac
    13861 tctgctcttt tttctttgct gtatttattc caaaaagtgt gtaaagcggg cagaacccaa
    13921 tcagcgaggt tagagtaagt acaaaagcaa caataagagc aacgattccc agaatgacgg
    13981 agaaaatacc cttgtaaaac agaacaatca gcacgatagc caatgctaag cgtactactt
    14041 tgtcggtcga acctacattt gttttcataa gtttgagatt tgatttttat tttaaaacaa
    14101 atattcgagc ccttatgttt aaattttcat taccaaatct tgtgaaaata tttttttgga
    14161 atgtttttga tagatatttt aaaatgttta gaacaattaa ataaaaatca gcaagttaga
    14221 ataattgttt aaattgaaac aatttaaagt tttcttgctg ctaaaaagaa ttgcagttga
    14281 accaatactt tttgtttcgc tgcagaaaat aagtccaaaa ttttgtgtaa gtttgcattt
    14341 ggttcacgaa gttctcggat atttatcatt ttaatgagct tatggaaatc aaaaatttaa
    14401 aaattgccat tattggagcc ggaaatatgg gtggagcatt ggcacgcggc tggtcaaaat
    14461 cagacatttt aagtgctgaa aatatcgcca ttagcgatat ttctcaagaa aagttggatc
    14521 aattgactgc ttttaatcca cattttaccg taagtacttc taatagggaa atcatcaggc
    14581 aggccgatat tgttttgctt gctatcaagc cgtggctggt cgaaacggtt gccaaagaaa
    14641 taagcaagag catcgattat aaaaagcagg taatcatttc cattgcggca ggtgttagtt
    14701 ttgaaaccct gacgcatttt tttgattctg atggagtgtt ttttcgtgtt attccaaatg
    14761 tggcggtcga aataggacag ggtgtttctg ttgtttcatc gttcaatgcc tgcaaagaac
    14821 accaacagtt gttactggaa atttttaatg ttttagggaa aacatttttc gtgtccgaaa
    14881 atcaaatgga tgcttttatg gcattaactt catgtggcat tgcttatgct tttcggtatg
    14941 tgcgggcttc ggtagccgga gctgttgaaa tgggcatttc gcctcagatg gctcaggaag
    15001 cggttattca aaccgtaaga ggtgctgtgg atttgcttga actccatcag tctcatccag
    15061 aaacggaaat agataaggtt actacgcccg gaggctttac catcaaaggt ttgaacgaaa
    15121 tggaagccaa tggattttca aatgctgtta tcaaagggtt gaaagcatct tattccaaat
    15181 gaaaaaatag attctgcaaa aaacaatttt ttttaagttt tacatccatg aatattcaaa
    15241 tcaaacaaat tgccgaaaga ttacgtgcat tgcgcgaatc acttgaactg actcccgaac
    15301 aggctgcaga aaaatgtggt gctacaacag aagaatatca acaatacgaa tcgggcgatt
    15361 acgatatacc gatgagtttt atctgtcagg ctgccgattc tttcggcgtt gcaactactg
    15421 ctcttcttgt tggcgatgat cctcacgcac aggttttttt cgttacccgt aagggaacgg
    15481 gagcaagcgt agaaagaaca aaagcttaca aatatcaggc acttgcttac ggttttcgtc
    15541 atcccaaggc agaacctttt gaggttaccg tagaacccaa cgacaatccg cttacattga
    15601 atacccattt caatcaggaa tttaattatg tgctggaagg cactttgcta ttgcgcatcg
    15661 ccggcaacga ccttattctg aacgaaggcg acagcattta ctttgatgca acacgcccac
    15721 atggaatgaa agctttgaac gggaaaagag tcaaatttct ggctgttatc atttaattaa
    15781 tagtctcgaa agaaagcttt tttcggataa aaaataattt taaaagacgt agcaaatttt
    15841 attaattgaa cttatcgtta gtaatttatt ctattcaaaa tatgttggaa aaatatttaa
    15901 aacaaactga atttaaggat tttgatgatt ttaaggctaa ttatcaattg atcgttcccg
    15961 agcattttaa ttttgcttac gacgtgatag acgagtgggc tgccaaagag ccggacaaac
    16021 tcgccatgat ttggaccaat gatcagggag aatgcagaaa attttctttt gctgaaatga
    16081 aaaaatacag tgaccagact gcttcttatt tccaaagttt gggtatagga aaaggcgatg
    16141 tggtgatggc catactcaaa cgtcgatatg agttctggtt tactattctg gctttacata
    16201 aattgggcgc tattactatt ccagacacgc atttgcttac gccgaaagac ttgatctacc
    16261 gaaacaatgc tgctgatatt aaggcaatag ttgcagttgg agaagaaccg attgtaagcc
    16321 acattaacga ttcaatggct gaatccccta ctgttgaaat gctgatttcc attgggccta
    16381 tcgtgccgga aaattggctc gattttcaca agggaattga tgaggcgact ccctttacac
    16441 ccattcatca tgtgaatgaa aataccgatc cgttgattat cagttttacg tccggaacta
    16501 caggtaatcc aaaaatggtg ttgcttgatt gtctttatcc gttgggacat attattacag
    16561 ccaaatactg gcacaatctg catgaaaaca gtattcatct gacgatagcc gataccggat
    16621 ggttgaaagc actttggggg aaaatttatg gacagtggat tgtgggtgct tgtgtatttg
    16681 tatatgacca cgaaaaattt actcccgcaa atatgctcga aatgatcgag aaatatcatg
    16741 taacttctct ttgtgcgcct ccaaccattt tccgttttct gatcagggaa gacattagca
    16801 agtatgattt gtcttctctg gaatatgcta caacggcagg agaagccctg aatgcttccg
    16861 tttacgaaaa actttacgag ttgacaggca ttaaactgca cgagggttac gggcaaagtg
    16921 aaacgacagt ttgtgtatat accactcctt ggattgaacc aaaacccggt tcgatgggat
    16981 tgccgggacc tcattacgat gtggatattc aaatgccgga tggacgatcg gctgaagtgg
    17041 gtgaacaagg ggaaattgta atcaagctgc acaaaaatta tcctgtgggt ttgttcaaag
    17101 gttattatcg taacgaagaa ctgacacgtg aagctttcag gggaaatgtt tatcataccg
    17161 gcgatatggc ttggaaagat gaagagggtt atttctggtt tgttggtaga acggatgatg
    17221 tgatcaaaag ttccggttac cgtatcgggc ctttcgaagt ggaaagtgca ctgatgactc
    17281 accctgcagt tgttgaatgt gccattaccg gagtacccga cgaattgcgc ggtcaagtag
    17341 tgaaagcaac ggtaatactt gccaaagaat acaaggataa agcaggagag gagcttgtaa
    17401 aagaacttca aaatcatgtg aaaaaagtta ccgctcccta taaatatccc cgtattatcg
    17461 aatttgtgga cgaactgccc aaaaccatca gcggaaaaat tcgccgtgtg gaaataagag
    17521 aaaaggataa acagtaaaaa aactgttgtt ttgatggaga gagagaagag aaaataatta
    17581 tttgatgttt gtcgatatat gaaaattata aatctgtccg ataacaattc aattttaaat
    17641 cagtttataa aagaaatccg gtcggtagaa attcagaaag attccatgcg tttcaggcgg
    17701 aatatggaga gaattgggga aatcatggct tatgaaatca gcaaagctat ggattatgtg
    17761 gaggaaaaag ttcagactcc tttagggatt gctcccgtga aagttatcga cgaaaaagtg
    17821 gtaatttcta ctattcttcg tgccggcctt cctttccatc aaggtttttt aaattatttc
    17881 gatggctcgg aaaatgcttt tgtctcagct tacagaaaat acagggatgc ggaaaatttt
    17941 gaaatttttg tagaatacat tgcttctccc ggtttggaag gaaaaacgtt gattctcact
    18001 gatcccatgc tggctaccgg aagctcgatg gagcagactt tttcagcgct aaccaccaaa
    18061 ggatttccct ctaaaattca tatcgctacg gttattgcca gccgccatgc gataaattat
    18121 ctcactaccc atttccctga aaatataacc actatctggg ctgctgccat cgatcccgaa
    18181 ataaacagtc attcttacat cattcctggt ttgggggatg ccggagattt ggcttttggc
    18241 gagaaactgt aaataaaaaa gctcttcacc tgtcgatgaa gagctttaaa aattcatttt
    18301 tatcggttca cgccagcttg ttcacatgaa tagccaattt cgattttaag ttgttggctt
    18361 tgttcttgtg aatcatattg cgtttagcca gcttgtcgag catggaagaa acttgtggca
    18421 acaattctgt ggctttttct ttatcggtag ttgtacgcaa tcttttaatt gccgtacgtg
    18481 ttgatttgat gtaatagcgg ttgtgaagcc tttttttgtt tgcctgacga attcttttaa
    18541 tagctgaatt attgttagcc attgtttttt gagatattaa agtgttgtaa tatggtagcc
    18601 catagcagaa tcgaactgct ctttagagaa tgaaaatctc tcgtcctaac cgatag
//
LOCUS       DAOSCH010000029        18640 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2068_length_18640_cov_6.607264, whole genome shotgun sequence.
ACCESSION   DAOSCH010000029 DAOSCH010000000
VERSION     DAOSCH010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18640
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2068_length_18640_cov_6.607264"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="PLZ54_03760"
     CDS             <1..368
                     /locus_tag="PLZ54_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HOJ66084.1"
                     /translation="MVKEEKYYVLIGPGRWGSSDPWLGIPVKWPNISNARVIVETELS
                     HYRIDPSQGTHFFQNLTSFGVAYFTVQPFQHLGFYDLDFLAQQPAIYESEYIRHLHFD
                     KPLVVKVDGKKNIGVIMKP"
     gene            complement(504..950)
                     /locus_tag="PLZ54_03765"
     CDS             complement(504..950)
                     /locus_tag="PLZ54_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOJ66085.1"
                     /translation="MEPIILAYIGIGLMVGLSGVGSAIGVTITGNATVGALKKNPDAF
                     GNYMVLSALPGTQGLYGFLGYFLLSSHLVPNMTWGASAAVFGASLALALTGLLSAIRQ
                     GQVCANGIAGIGSGYDVFGRTLILAVFPELYAIVSLAAVFMIGNAI"
     gene            complement(1019..2842)
                     /locus_tag="PLZ54_03770"
     CDS             complement(1019..2842)
                     /locus_tag="PLZ54_03770"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOJ66086.1"
                     /translation="MTRYTFLVYHKQYHDFLEKMREVGVVHVIEKVEGIAENDELREK
                     MLLSSRMKIALKQMEKLIPQDASPLPADLSVDGMELLKKIENKFAEKELLIQKLQLAE
                     KERDRMEMWGIFDYQLIENLQKAGYEINFFSCNVRKFDQEWEIRYNAFEIDNIGSTRY
                     FVTITHTGEIIEIDADPVKIGTKNVLQIDEDIQQIHQEIDNINRQLITLSVENYNSLK
                     AAQVKVIENMDFARVLLNTEPTVENKVMLLEGWCPEDAEEKLKEFLNETDIYYETSKP
                     EEDEKVPVKLKNNRFSRLFEFIGNLYDLPNYHELDLTPFFAPFYMLFFGICFGDAAYG
                     LLLLIASLYFRKKADAALKPILSLAALLGISTVIMGFVSGTFFGFNLLEMDVPWLASF
                     KAFMLDSNQLFYTSLLLGVVQILFGMVIKAIGKVKRYGWPASFSTWGWLILLIGCGST
                     FVLSSFAGLSPQTAKWLYIIFGSAGGALVFLLNNIKRNPLINIGSGLWDTYNTATGLL
                     GDVLSYIRLFALGISGAVMGFVFNDLAINLSGNIPIVSTLIMLVIMIFGHGINIFMCG
                     LGAFVHPMRLTFVEFYKNAGFEGGGKKYKPFSHFKEEKSII"
     gene            complement(2851..3456)
                     /locus_tag="PLZ54_03775"
     CDS             complement(2851..3456)
                     /locus_tag="PLZ54_03775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOJ66087.1"
                     /translation="MSIQFQYNKTSLQQLEKNLKIRVRALPTIKNKESALRTEVKKAK
                     EEIKRIENELEQRIAEYDKMLALWGEFDTTLLEVEEVKMNIKKIAAVRVPILEEVVYK
                     TKPFSLFNSPKWYSDGLALLKKLAQIGIEQEFFIQKLSLLEFARKKTTQKVNLFEKVQ
                     IPGYEDAIRKIKRFMEDEENLSKSSQKIVKSRQELIKKENL"
     gene            complement(3512..4843)
                     /locus_tag="PLZ54_03780"
     CDS             complement(3512..4843)
                     /locus_tag="PLZ54_03780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOJ66088.1"
                     /translation="MTTKAFQKIYTKITKITKATCSLHASGIGNDELALVNGKLAQVV
                     KILGDEVTLQVFEGTEGIPTDAEIVFLGKAPTLKVSDQLAGRFFNAFGDPIDDRPEIE
                     GEEREIGGPSVNPVRRKQPSELIATGIAGIDLNNTLVSGQKIPFFADPDQPYNEVMAN
                     VALRAKTDKIILGGMGLTNDDYLYFKNVFNNAGVLDRIVSFVNTTEDPPVERLLIPDM
                     ALTAAEYFAVDKNEKVLVLLTDMTLYADALAIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPAGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRRDSDIGKVIVDPFR
                     SLSRLKQLVIGIKTREDHPQVMNAAVRLYADAANAKTKLENGFDLTDYDERALAFAKD
                     YSDRLLAIDVNIDTTAMLDTAWKLFAKYFSPTEVNIKQEFIDKYWKQKQHE"
     gene            complement(4918..6678)
                     /locus_tag="PLZ54_03785"
     CDS             complement(4918..6678)
                     /locus_tag="PLZ54_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOJ66089.1"
                     /translation="MPTKGKVKGIVSNLVTVSVDGAVSQNEICFIELGSEKLMAEVLK
                     VNGSDVFVQVFESTRGLKIDNEVTFEGHMLEVILGPGLLSKNFDGLQNDLNKLTGIFL
                     KRGERNFPLDEDKKWKFKPIAQVGDEVEAASWLGEVDENHQPHKIMVPFVMEGSYKVK
                     SIAPAGEYTISETIAIITDAHGKDFEINMIQKWPVKKAINVHAEKPRPYKLLETGVRV
                     IDTANPIVEGGTGFIPGAFGTGKTVLQHAISKQAEADIVIMAACGERANEVVELFVEF
                     PELKDPHTGRFLMERTIIVANTSNMPVAAREASVYTAMTIAEYYRAMGLRVLLMADST
                     SRWAQALREMSNRMEELPGQDAFPMDLSAIIANFYSRSGFVKLKNKQTGSITFIGTVS
                     PAGGNLKEPVTESTKKVARCYFGLEQARADSKRYPAINPIESYSKYIEYPEFDEYISE
                     RISPQWISMVNDMRTLLQRGKEVQEQINILGDDGVPVDYHITFWKSELIDFVILQQDA
                     FDDIDAVTPIERQHYMLKLIMDICKSDFNFVNFTDVMDYFKRIINVCKQMNYSEFHSQ
                     AFESYYNELQNILAERKIKQ"
     gene            complement(6765..7622)
                     /locus_tag="PLZ54_03790"
     CDS             complement(6765..7622)
                     /locus_tag="PLZ54_03790"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOJ66090.1"
                     /translation="MNYYCLIAGLPDLSPDDKKTTFTLADFKNELLELLDTSDVEFLR
                     LIFAQYDNQNFLAFLIDRDAFLQPLGNLNYDDWVEIVQLLKETENPKDKRILPYIQHF
                     YSVYQDEEFWAEGISKEDYLTGLYYDFALQCNNSFLKSWFEFSLNINNLLTAIACRKY
                     GLNPHNYVVGSNTIAQQLRTSNARDYGLTGTFEYLEQVLQIAEEPDLMEREKKIDLLK
                     WQWMDEHTFFYYFSIEKILAYILKMQMIERWQPLTPEKGNVIFRELIEKMKQSVEFEE
                     TLQAGEKIR"
     gene            complement(7700..8290)
                     /locus_tag="PLZ54_03795"
     CDS             complement(7700..8290)
                     /locus_tag="PLZ54_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66091.1"
                     /translation="MNTKLQELTEKIYLEGVEKGNAEAQSIIEEAQKKADSIISSAQE
                     KAARIIAEAEAKAAELEENTKSELRLYAQQSLNALKTEITNLICGKIVDDSVKAAMVD
                     KNFMQQVILQLAKEWSKKESIVIEAPDAKALTEYFLANAKNLLNQGVTITQADKSKAT
                     FTIRPDKGGYKINFGEEELTEYFKDFLRPKLIEMLF"
     gene            complement(8682..9350)
                     /locus_tag="PLZ54_03800"
     CDS             complement(8682..9350)
                     /locus_tag="PLZ54_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66092.1"
                     /translation="MKKTIKLAIALSILTFISCKDNTPNQPYQYELNPAYTWGYVVFY
                     GDYYADYLNKNNVLTVNLFTDSLKINNQNELLGYGQYLFLEDVFVAPSDTLLPEGIYR
                     VDNSGEPFTIAPGINDTIDNQVYTLGAVINYYEPNPLKSTQKLIVGGKMTVSRQGENY
                     SLNCDFVLQDSLKLKGIFSGTLPHFDESLQREERNSIYIFKENSLVKEKKTIYKFFGK
                     KKNY"
     gene            complement(9432..10721)
                     /locus_tag="PLZ54_03805"
     CDS             complement(9432..10721)
                     /locus_tag="PLZ54_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ66093.1"
                     /translation="METIHPNQTKGDNTSGGFRFATWVARRYLFSRKSHNAINIISGI
                     AALGVGIGTMALICVLSVFNGFDSLIQGMFSTFDPDLKITVSQGKTFDINTPEWEKVK
                     KLNNVAYFTEVIEENALLRFRDKQMPATIKGVSDDFQKMTKIDSIMFDGKFLLYDGAF
                     QRAVPGVGVASILGLGANFIDPLYIYAPKRTSHINLLHPENSFNQIGTFVSGIFSVKQ
                     LQYDDHYVLVSIELARDLFEYQPNTVSSIELKLKDNIKTLDAQKKIKNILGGNFEVKD
                     RYEQQEDFFKILKIEKWITYLILCFILLIASFNIISSLSMLIIDKKQDIQTLRNLGAE
                     QQLIRRIFLFEGWLISAVGAVLGIIMGLLLCLLQQHFGILKLGTGYVVDVYPVLVNFW
                     DVLLVFMTVLLMGFLAAYYPVRYIDIHDAPSPVNVEL"
     gene            complement(10767..11147)
                     /gene="rbfA"
                     /locus_tag="PLZ54_03810"
     CDS             complement(10767..11147)
                     /gene="rbfA"
                     /locus_tag="PLZ54_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOJ66094.1"
                     /translation="MDNTRQQKVARLLQKELGEIFLLYAREMHGIIISVTNVRISPDL
                     SIARIHLSIFPGKQSAEVLEKINNDQKNIRFDLGKRVKHQLRIIPELFFYLDDSLDYI
                     DHIDQLLKNDSPSDKKDHSQENNQ"
     gene            complement(11264..12091)
                     /gene="rhuM"
                     /locus_tag="PLZ54_03815"
     CDS             complement(11264..12091)
                     /gene="rhuM"
                     /locus_tag="PLZ54_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOJ66095.1"
                     /translation="MNNEIILYQSDNAVELEVILENETIWLTQAQIAELFGTRRQAIT
                     KHLKNIFDSGELSENSVSSKMELTAKDGKNYLTNFYNLDAILSVGYRVNSKNATQFRI
                     WANKVLKDYLLKGYAVNQRIENLERRVSATEDKIDFFVKTALPPVQGIFYDGQIFDAY
                     KFVADLVKSATKSIVLIDNYLDESVLILLSKRKENVKCQIFTNKISAQFKLDLEKFNA
                     QYPPLEVKEFSKSHDRFLIIDETIFHIGASLKDLGKKWFAFSKINLDAKLMMEMMSK"
     gene            complement(12136..12969)
                     /gene="rhuM"
                     /locus_tag="PLZ54_03820"
     CDS             complement(12136..12969)
                     /gene="rhuM"
                     /locus_tag="PLZ54_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008781192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HOJ66096.1"
                     /translation="MNANNEIILYQSDNAVQLEVMLENETVWLTLNQMSILFERDKSV
                     ISRHISNIFKEQELDKNSVVAKNATTASDGKTYLIEYYNLDVIISVGYRVKSKRGTQF
                     RIWANKVLKDYLLKGYAVNQRIENLERRVSATEDKIDFFVKTALPPVQGIFYDGQIFD
                     AYKFVADLVKSATKSIVLIDNYLDESVLILLSKRKENVKCQIFTNKISAQFKLDLEKF
                     NAQYPPLEVKEFSKSHDRFLIIDETIFHIGASLKDLGKKWFAFSKINLDLQDILKKIY
                     Q"
     gene            complement(13002..14468)
                     /gene="cysS"
                     /locus_tag="PLZ54_03825"
     CDS             complement(13002..14468)
                     /gene="cysS"
                     /locus_tag="PLZ54_03825"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOJ66097.1"
                     /translation="MENNLYIYNTLHRKKELFVPLHAPHVGMYVCGPTVYGDPHLGHA
                     RPAITFDVLFRYLKHLGYKVRYVRNITDVGHLENDADSGEDKIEKKARLEQLEPMEIA
                     QYYANRYHKAMEALNVLSPSIEPHASSHIPEQIEFVKKILDAGFAYESEGSVYFDVEK
                     YDKKYHYGKLSGRNIDELLETTRMLEGQEEKRRSVDFALWKKASPEHIMRWKSPWSEG
                     FPGWHLECSAMSTKYLGEEFDIHGGGMDLLFPHHECEISQSTAALGKESVRYWMHNNM
                     ITINGQKMGKSLGNFITLEEFFSGNHPLLSQAYQPMTVRFFILQAHYRSTVDFSNEAL
                     QASEKGLERLMEGYDRLQKLMPSDISTSDISGLRQRCEEAMCDDLNTPIVIAHLFEAV
                     KFINLVYDGKATVSSADLEELKDVFRVFVEDLLGLKKEDENFAKYEALKKAVDLLLQI
                     RLEAKRNKDWATSDKIRNELTALGFEIKDTKDGFEWKI"
     gene            complement(14515..15249)
                     /locus_tag="PLZ54_03830"
     CDS             complement(14515..15249)
                     /locus_tag="PLZ54_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOJ66098.1"
                     /translation="MSGFAKWIGGGLGWALGGPIGALIGFAIGSFIGAKIETVEKSQK
                     RRTTTTSGDFKISLLVLIACMMKADGHVKKSELEIVKRFLVDNFGEEGALEALSILKN
                     LLEKDIDERQVAEQIYRYTNYSTRLELIHLLFDIAYADHQVSTSELTLIERIAGIFRI
                     SGLDFESLKAPYIKQVDINWAYKALEIEPSASVDEIKKAYRKMAMKYHPDKVNTLGED
                     VKKKATEKFRSINEAYETLKKERKFT"
     gene            15534..16019
                     /locus_tag="PLZ54_03835"
     CDS             15534..16019
                     /locus_tag="PLZ54_03835"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOJ66099.1"
                     /translation="MKLSKFILAFLVLSALVFALPACKSKQKVVEIPGANAKAGTSTS
                     ENQQKTTSPVIVTQPTTSTENVPEITRSESFSLAEGETNQSAMNYKYHVVVGSFRNQS
                     NAKGLQRTLSNEGYNPVIVINEQGMFRVIMNSFNTYNEAHVFISKIQSRFPDAWVLIK
                     K"
     gene            16753..17172
                     /locus_tag="PLZ54_03840"
     CDS             16753..17172
                     /locus_tag="PLZ54_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOJ66100.1"
                     /translation="MATHITKTIWRKDNIFDTDVDGHIITIDLGKEAGGEDAGPRPKK
                     LLLVAAAGCSGLDVVSLLKKMRVELKGFDIQIEAQMSEEHPKQYTSMKVIYEFEGDNL
                     PMEKLEKAVTLSYEKYCGVMAMYKKAIPISWEIRIKS"
     gene            17294..18556
                     /locus_tag="PLZ54_03845"
     CDS             17294..18556
                     /locus_tag="PLZ54_03845"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOJ66101.1"
                     /translation="MQTMDSFNFAGKKAFVRVDFNVPLDENFNITDDTRIRAAIPTLK
                     KILNDGGCVIIASHMGRPKKNPDPKYSLKYILPHVSKLLGIEVKFAPDCIGPETAAMV
                     SALKPGEALMLENLRFYEEEEGKPRLPETATEEEKAAAKKELKERQKKFTATLASYAD
                     CYVNDAFGTAHRAHASTALIADYFDADHKMFGYLMEKEVAAVEKIMNNTEHPFTAIMG
                     GSKVSSKIDIIENLLNKVDNLIIGGGMTYTFVKALGGKIGSSICEEDKLDLALSLLEK
                     AKSKGVNMVLAVDAKIADAFSNDANTKFVPVNEIPDNWEGLDIGPETEKLFADVIKSS
                     KTILWNGPVGVFEFDNFTHGSLAVAQAIVEATKNGAFSLIGGGDSVACINKFGLADGV
                     SYVSTGGGALLEAIEGKVLPGIKAIRGY"
BASE COUNT         5566 a         3571 c         3273 g         6230 t
ORIGIN      
        1 aaatggttaa agaagaaaaa tattatgtgt tgataggtcc gggacgatgg ggctcaagcg
       61 acccttggct gggtattccg gtgaaatggc ccaacattag caatgccaga gtaattgtgg
      121 aaacggaatt atcgcattat cgaatcgacc ccagtcaggg aacgcatttt ttccaaaatc
      181 tgacttcttt tggtgtagcc tattttacgg tacaaccttt tcagcatctc ggtttttatg
      241 acctcgattt cttggctcag caaccggcaa tatacgaatc ggaatacatt cgtcatttgc
      301 atttcgataa gccgctggtg gtgaaagtgg atgggaaaaa gaatatcggc gtgatcatga
      361 aaccgtaaaa ttttttctga aatgctaaac ctaatgaaaa catcgaacaa gacaaaaatc
      421 ctgttcgatg ttcacaatga gaaaaaataa ttgatttgag gttgttttat tgtgtttcag
      481 agatattttt tcagattgga atatcagata gcatttccaa tcataaaaac agctgccaat
      541 gaaacaatgg cataaagttc aggaaatact gccagtatta atgttcgacc aaagacgtca
      601 tatcctgatc caataccggc tataccattt gcacaaactt gaccttgtcg tatggcagaa
      661 agaagccctg taagggcaag tgccaaactg gcgccaaata ctgctgctga agcaccccac
      721 gtcatgttgg gaactaaatg tgatgagagc aaaaaatatc caagaaatcc atataatcct
      781 tgtgtacctg gtaatgcact taaaaccata tagtttccaa atgcatcggg attttttttc
      841 aaagcaccga cagtggcatt gccggtaatc gttacgccaa tagcacttcc tactcctgaa
      901 agtcccacca ttaggccaat accaatgtaa gctaaaataa taggttccat aatttttttt
      961 ttaaatttgt tttttaaatg tgatatttct ttttttagtt ggattttatt ccgaaaaatt
     1021 atatgatgct tttctcttct ttaaagtgcg aaaaaggttt atattttttc ccgccgccct
     1081 cgaagcctgc atttttataa aattctacaa aagtaagacg catgggatga acgaaagcac
     1141 ccagaccaca cataaatatg ttgattccat gtccaaaaat catgataacc aacattatga
     1201 gtgtgctcac tataggtatg ttgccgctta aattgatggc aagatcgttg aaaacaaaac
     1261 ccataactgc accagaaata cctagtgcaa ataatctgat ataagacagt acatctccca
     1321 gtaagccggt agcagtgtta taagtatccc ataaccctga tcctatattg ataagtggat
     1381 ttctttttat attgttcagc agaaatacca aagctccacc tgcactgcca aaaattatat
     1441 acaaccattt ggcagtttgg ggcgaaagcc ctgcaaatga cgaaaggaca aaagtacttc
     1501 cgcaaccgat caacaaaata agccatcccc acgtagaaaa ggaagcaggc cagccataac
     1561 gtttaacttt tccaattgct ttaattacca tcccaaaaag tatttgaacc acgccaagca
     1621 gtagcgatgt gtaaaacaat tgattggaat ctaacataaa tgctttaaaa gatgcgagcc
     1681 aggggacatc catttccagt aagttaaatc caaaaaaagt tccactgaca aagcccataa
     1741 taacggtaga aattcctaat agggcagcca aactcaatat tggttttagt gctgcatctg
     1801 ctttttttct gaaataaaga gatgcaatca gtaaaagaag tccgtacgct gcatcgccaa
     1861 agcaaattcc aaaaaaaagc atgtaaaatg gtgcgaagaa aggggtcagg tctaattcat
     1921 gataattagg taagtcatag aggttgccta taaattcaaa caagcgcgaa aagcgattgt
     1981 ttttcaattt tacgggtact ttttcgtcct cttcaggttt gctggtttcg tagtaaatat
     2041 ccgtttcatt aagaaattct ttgagctttt cttctgcatc ttccggacac cagccttcca
     2101 aaagcattac tttgttctcc acagttggct ctgtgttaag caacactctt gcaaaatcca
     2161 tattttcaat aacttttact tgagctgctt ttaatgagtt gtaattttca acagaaagtg
     2221 ttatgagttg gcgattaatg ttatcaattt cttgatgtat ttgttgaata tcttcatcta
     2281 tttgcaatac attttttgtg ccgattttta cgggatcagc atctatttca ataatttcgc
     2341 ctgtatgagt tatggttaca aaataacgtg tgctgccaat attatcgatt tcaaaagcat
     2401 tgtagcgtat ttcccattcc tgatcgaatt ttcgcacatt acaactgaag aaattaattt
     2461 cgtaacctgc cttttgcaga ttttctataa gttgataatc aaaaattccc cacatttcca
     2521 tgcggtctcg ttctttttct gcaagttgga gtttttgaat taataattct ttttccgcaa
     2581 atttattttc aatttttttc aaaagttcca ttccatcaac cgatagatcg gccggtagag
     2641 gagaagcatc ttgaggtatc aatttttcca tttgtttgag agctattttc atgcgcgaag
     2701 aaagtagcat tttttcacgt aattcgtcgt tttcggcaat gccttccact ttttcaatta
     2761 cgtgaacaac acccacttcc ctcatttttt cgaggaaatc gtggtattgt ttgtgataca
     2821 ccagaaaagt atatcgtgtc atttgcaaaa tcataaattt tccttcttta taagttcctg
     2881 acgcgattta actatttttt gagacgattt ggataaattt tcttcatctt ccatgaaacg
     2941 tttgatttta cgaatagcat cctcgtagcc gggaatttgt actttttcaa acaaattgac
     3001 tttttgggtg gttttttttc gagcaaactc cagcaggctt agcttttgta tgaaaaattc
     3061 ctgctctatt ccgatttgag ccagtttttt aagaagggcc aatccatcgg aataccattt
     3121 tggagaatta aataggctga aaggtttggt tttataaact acttcttcca atatcggtac
     3181 tcgtacagct gctatttttt ttatgttcat ctttacttct tctacctcca ataaagtagt
     3241 atcaaattcg ccccatagag caagcatttt atcgtattct gcaatacgtt gctccaattc
     3301 attttcaatt ctttttatct cttccttagc ttttttcact tcggtacgta aagcgctttc
     3361 tttgttttta attgtgggta aagcgcgcac tcttattttt aaattttttt caagctgttg
     3421 cagcgatgtt ttattatatt gaaattgtat ggacataaaa cgtttattta actaaatgat
     3481 aatttttttt agcaatttaa caaatgttga tttattcatg ctgtttttgc ttccaatatt
     3541 tatctatgaa ttcctgttta atgttcacct ctgttgggct gaaatatttg gcaaagagtt
     3601 tccatgccgt gtcgagcatg gcggttgtgt ctatattgac atcgatagcc aataatctgt
     3661 cggaatagtc tttggcaaaa gccaatgctc tttcgtcata gtcggtgaga tcaaatccat
     3721 tttcaagttt tgttttggca ttggcagcat cagcataaag tcgaacagct gcgttcatta
     3781 cctgtgggtg atcttcgcga gtttttatgc cgataaccag ctgtttgagg cgcgacaagg
     3841 aacggaaggg gtcaacaatt actttgccta tatccgaatc gcgacgcagg aacaattgtc
     3901 cttcggtaat ataacctgta ttgtcgggaa tagcatgggt aatgtcgcct cccgagagag
     3961 tagttaccgc gataatggta atagaacctc cggcgggaaa ttgtacggct ttttcatata
     4021 tttttgccaa gtcggaatac agtgagcccg gcatggaatc tttagaagga atctgatcca
     4081 ttctattgga tactatagcc aatgcatcgg catataatgt catatcggtg agcaaaacca
     4141 atactttttc attcttgtcg acagcgaaat attctgcagc ggtaagtgcc atatcgggaa
     4201 taagtaaacg ctctaccgga gggtcttctg tggtattcac gaaactgaca atccggtcta
     4261 gtacgccggc attgttgaaa acgtttttga aatagagata atcatcattt gtaagtccca
     4321 ttccccctaa aataatttta tcggtttttg cacgcaaggc aacatttgcc attacttcat
     4381 tgtatggttg atcgggatcg gcaaaaaatg gtattttctg accggatacc agcgtgttat
     4441 tcaggtcgat gcccgcaatt cccgttgcaa tgagttccga aggttgtttc cgtcgtacgg
     4501 gattaactga tggacctcca atttctcttt cttcaccttc aatttcgggg cggtcatcaa
     4561 tagggtctcc aaaagcattg aaaaaacggc ctgccagttg atcgcttact tttagggttg
     4621 gtgctttccc gagaaagaca atttctgcat cggttggtat tccttcagtt ccttcaaaaa
     4681 cttgcaaggt tacttcatcg cctaagattt taactacttg agccaattta ccgtttacta
     4741 aagccagttc gtcattgcca atgccacttg catgaagaga acaggttgct tttgtgattt
     4801 tggtaatttt ggtataaatt ttttgaaatg cttttgttgt cattttttct cagattatta
     4861 ttttctcttt ttccgggaaa tttttttctt tttagtcttt atatttgtcc aaaaaaatta
     4921 ttgttttatc ttcctttctg caagtatatt ttgcagttca ttgtaataac tttcgaaagc
     4981 ttgggaatga aattcggaat agttcatttg tttgcaaaca ttaataattc gtttgaaata
     5041 atccataaca tcggtaaaat ttacaaagtt aaaatctgat ttgcagatat ccataatcag
     5101 ttttaacatg taatgctgtc tttctatcgg agttacggca tctatatcgt caaaggcatc
     5161 ttgctgaaga ataacaaagt ctatcaattc ggatttccag aaagtaatgt gataatcgac
     5221 gggaacacca tcatctccca gaatattgat ttgttcctga acttctttac cgcgttgaag
     5281 aagtgttcgc atgtcgttta ccatactgat ccattgggga gaaatacgct ccgagatgta
     5341 ttcgtcaaat tcgggatatt caatatattt tgaataactt tcaatgggat tgatggccgg
     5401 atatctttta ctatcggctc ttgcctgttc aagtccaaaa taacaccggg ctactttttt
     5461 ggtgctttcc gttacaggtt ctttcagatt acctccagcc ggcgatactg ttccaataaa
     5521 ggtgatggaa ccggtttgtt tgttttttaa tttgacaaaa cccgatcggc tgtaaaaatt
     5581 ggctataata gccgacaaat ccatcggaaa agcatcttgt cccggtaatt cttccatgcg
     5641 gttactcatt tcgcgtaaag cctgagccca gcgtgaagta gagtctgcca tcaataacac
     5701 tcgaagtccc attgctctgt aatattcagc tattgtcatg gccgtgtaaa ctgatgcttc
     5761 acgggcggca acaggcatat ttgaagtgtt ggccacaata atggtacgtt ccattaaaaa
     5821 tcgacctgtg tgtggatctt tcaattcggg aaattctaca aacaattcaa ctacttcatt
     5881 tgcacgttca ccacaagcag ccatgataac aatgtctgct tcggcttgtt tggaaatggc
     5941 gtgttgcaaa acggtttttc ctgttccaaa agcaccggga ataaatcctg ttcccccttc
     6001 tacaattggg ttggctgtgt ctatgacgcg aacacctgtt tcgagcaatt tatagggtct
     6061 cggtttttcg gcgtgtacat ttattgcctt ttttacaggc catttttgta tcatatttat
     6121 ttcaaaatcc tttccatgtg catcggtaat tatggctatc gtttcagata tcgtatattc
     6181 gcctgccggg gcaatgcttt ttaccttata actgccttcc atgacaaagg gtaccataat
     6241 tttgtgcggt tgatggtttt cgtcgacttc ccccaaccat gaagcagctt caacttcatc
     6301 ccctacctga gcaataggct taaacttcca ttttttatct tcgtccagtg gaaaatttcg
     6361 ttctccacgt ttaaggaaaa tacctgttag tttgtttaag tcattttgaa gaccgtcgaa
     6421 atttttcgac agcaagccgg gccccagtat tacttctaac atgtgacctt caaatgttac
     6481 ttcattgtca atttttaatc cgcgagtgct ttcaaatact tgcacaaaaa catcagaacc
     6541 atttactttc agcacttcag ccataagctt ttccgaaccc agttctatga aacaaatttc
     6601 attttgtgat actgctccgt caacacttac ggttaccaag ttagaaacga ttccttttac
     6661 ttttcctttt gttggcatag ttgtttgttg tttccttatt ttttctcttt tgaactttca
     6721 acaaagagag gtctttttaa ttaaacatat tttacttatt agaattatct gattttttct
     6781 ccggcctgaa gagtttcttc aaattctaca ctttgtttca ttttttctat caactccctg
     6841 aaaattacat ttcccttttc gggagtcaat ggttgccagc gttctatcat ttgcattttc
     6901 agaatgtagg ccaatatttt ttcgatggaa aaataataaa aaaatgtgtg ttcatccatc
     6961 cactgccatt ttaaaagatc aatttttttc tctctttcca ttaaatcggg ttcttctgca
     7021 atttgcaaaa cctgttcaag gtactcgaat gtgccggtca aaccataatc gcgagcattg
     7081 cttgttctca gttgttgagc tattgtgttg ctgcctacaa cgtaattgtg gggattcagt
     7141 ccatattttc tacaggcaat ggcagtaagc aaattattga tattcaaact gaattcaaac
     7201 catgatttta aaaaactgtt gttgcattgc aaggcaaaat catagtataa tccagtgagg
     7261 taatcttctt tagaaatgcc ttccgcccag aattcttcat cctgatacac cgaataaaaa
     7321 tgctggatgt aaggcaatat tcttttgtct ttggggtttt ctgtttcttt cagcaactga
     7381 acgatttcta cccaatcgtc gtaattcaaa tttcctaaag gttggaggaa agcatccctg
     7441 tctatgagga aagccagaaa gttttgattg tcgtattggg caaaaatcaa tctcagaaat
     7501 tctacatccg aagtatcaag caattccaaa agttcgtttt tgaaatcagc gagagtaaat
     7561 gtagttttct tatcatcggg agacaagtcg ggaagtccgg caataagaca gtaataattc
     7621 ataactttct tttttgattc ttgaatttac gtattttttc ttgatgtttt tttgaaaaaa
     7681 tacggtatca tcatcttgtt taaaaaagca tttctattag tttggggcgt aaaaaatcct
     7741 tgaaatattc tgtcaactct tcttctccga aatttatttt gtatcctccc ttgtccggtc
     7801 gaatagtaaa ggttgctttt gatttgtcgg cttgggttat ggtaacaccc tgattcagca
     7861 aattctttgc attggctaag aaatattctg ttaaagcttt ggcatcggga gcttcaataa
     7921 ctatagattc ttttttagac cattctttgg caagttgaag aataacttgt tgcatgaaat
     7981 ttttatcgac catagctgcc ttcactgaat cgtcaactat ttttccgcaa atcagattgg
     8041 taatttcggt ctttagtgca ttgagtgatt gttgtgcata tagcctgagt tcagatttgg
     8101 tattttcttc gagctcagca gcttttgctt cggcttcggc tattatgcgc gcagcttttt
     8161 cttgagcgga ggaaatgatc gaatctgctt tcttttgagc ttcttcaata atagactgtg
     8221 cttcagcatt ccctttttct actccttcca aataaatttt ttcggtcaat tcctgcagtt
     8281 tagtattcat atacaaagta tttttctgat gttttatatt gcaaatttaa tcttttctgt
     8341 ctaaatcaaa acgtttttag gaaaatttaa aaaatgaaaa cgcatattcc attttatatt
     8401 ccagaattgt ttaaaaaaat attttttgag gcttgttgca gggtctttta gttaaaaaag
     8461 aaaattttac gagcaattaa ctgcataatg tgttacctcg aaaattttaa acaaatttat
     8521 tctcgagcga agagtaactg cacactaaac aacagtccga atttacaact atccggtttt
     8581 tataaaaata gggttagatt tttaaaaata tgatttgtaa aattaaatga tgaataaaca
     8641 atgtaaaagt aaagtcaacc gaatttaatt tatttaggtt gctaataatt cttctttttc
     8701 ccaaagaact tatagattgt tttcttttcc tttactaaag aattttcctt gaaaatataa
     8761 atagaatttc tttcctctct ctgcaatgat tcgtcgaaat gaggaagagt gccggaaaaa
     8821 attcctttca gtttcaggga atcttgcaga acgaaatcac aattcagtga ataattttca
     8881 ccctgacggc tgaccgtcat ctttccgcca acaataagtt tttgtgtgga tttcaatgga
     8941 ttgggctcat aataattaat gacagctcct aacgtataga cctggttgtc aattgtatca
     9001 tttattccgg gagcaatggt gaaaggttcg ccactgttat caactctgta aattccttcg
     9061 ggaagaagcg tatcggaagg agctacaaaa acatcttcaa gaaaaagata ttgaccataa
     9121 cccaaaagtt cattttggtt gtttattttt aacgaatctg tgaacaaatt gactgtcaaa
     9181 acgttgttct tattcaaata atcggcatag taatctccgt aaaaaacgac atagccccac
     9241 gtataagccg gattcagctc gtattgataa ggttgattgg gtgtgttgtc cttacaggag
     9301 ataaaagtca aaatgcttaa ggcaattgcc aatttaatcg tttttttcat gtttttttga
     9361 ttagtatata ttgatgaaat ctataaaaca gctggcttta taaacaaact ttcatagcac
     9421 ttctcgataa attataattc tacattcaca ggcgaagggg catcatgtat atcaatgtaa
     9481 cgaaccggat agtaggctgc cagaaaaccc attaacaaaa cagtcataaa taccagcaaa
     9541 acatcccaaa aattaaccaa aacaggataa acatccacta cataaccggt tccgagtttt
     9601 aaaatgccaa aatgttgctg cagtaaacag agcaacaatc ccattataat tcccagaaca
     9661 gcaccaaccg ccgaaattag ccatccttca aaaaggaaaa ttcttcttat cagttgctgc
     9721 tcagctccta aatttcgcag ggtttgaatg tcctgttttt tgtcaataat caacatggat
     9781 aacgaactga taatgttgaa actggctatc agcaaaataa aacataaaat gagataagta
     9841 atccattttt cgattttcag gattttaaaa aaatcttcct gctgttcata tctgtcttta
     9901 acttcaaaat tacctcccag tatatttttt atttttttct gtgcatctaa agttttgata
     9961 ttatctttta gctttaattc aattgatgag actgtatttg gttgatattc gaacaaatca
    10021 cgagccagct caatagaaac cagcacataa tgatcatcat attgcaattg cttaactgaa
    10081 aaaattcctg atacaaatgt tccgatttgg ttaaaactgt tttcaggatg gagcaaatta
    10141 atgtgtgaag tgcgttttgg ggcgtaaata tacaatgggt cgataaaatt ggctcccaat
    10201 cccaaaatgc ttgcaacgcc gacgccgggg acggctcttt ggaaagcccc atcataaagt
    10261 aaaaactttc catcgaacat aatgctgtct attttagtca ttttttgaaa atcgtcgctt
    10321 acgcccttaa tggtggcggg catttgcttg tctcgaaatc gcagaagagc attttcttca
    10381 ataacttccg taaaataggc aacgttgttc aactttttca ctttttccca ttccggagta
    10441 tttatatcga aagtttttcc ttgcgaaaca gtaattttca aatccgggtc gaaagtcgaa
    10501 aacatgcctt gtatcaagga atcgaaacca ttgaaaacgg aaaggacaca aataagtgcc
    10561 atagttccaa tgccaacccc caaagctgca attccggata ttatattgat ggcattgtgc
    10621 gatttgcgtg aaaacaaata tcgccgtgcc acccatgtag caaaacgaaa tcctcctgat
    10681 gtgttatctc ctttggtttg gtttggatga attgtttcca ttgtttcttc gggcaaaatc
    10741 tgctttcatt atccaatttt caataatcat tgattgtttt cttgcgaatg gtccttttta
    10801 tcggatggag aatcattttt taaaagttga tcaatatgat cgatatagtc aagtgaatcg
    10861 tccaaataga aaaacagttc aggaataatt cgcagttggt gtttcactct ttttcccaag
    10921 tcgaaacgaa tatttttctg gtcgttatta attttttcaa ggacttctgc tgattgtttg
    10981 ccgggaaaaa tactcaaatg aatgcgcgca atacttagat cgggacttat gcggacattg
    11041 gttacgctaa taatgatacc gtgcatttcg cgagcataga gcagaaaaat ttctcccaat
    11101 tctttctgaa gaagtcgcgc cactttttgt tgtcttgtat tatccataat aaattctcct
    11161 ttttctcagg caaatacgaa ccttcaattc cgttttttgc ctttttaatc tgtttttaat
    11221 tgtttaaccc cccaaatctt atactgtctc ttcaaattat attctatttt gacatcattt
    11281 ccatcatcag ctttgcgtcc agattaattt ttgaaaaagc aaaccacttt ttacccagat
    11341 ctttcagcga agctccaatg tgaaagatgg tttcgtcgat aatcaaaaat cgatcatgtg
    11401 atttggaaaa ttccttcact tcgagaggtg gatattgagc attaaatttt tccaaatcca
    11461 gtttgaattg cgctgaaatt ttattggtaa aaatttgaca tttaacattt tcctttcttt
    11521 ttgaaagcaa aatcagaacg ctttcatcca aataattgtc aatcaaaacg attgattttg
    11581 ttgccgattt caccaaatct gcaacaaact tgtaggcatc aaaaatctgt ccatcgtaaa
    11641 aaattccctg caccggagga agagctgttt taacaaagaa atcgatttta tcctctgttg
    11701 ccgaaacgcg tctttccaaa ttttcaatac gttggttgac cgcataacct ttcaacaaat
    11761 aatctttcaa aaccttgttt gcccatatcc tgaattgagt tgcattttta gaattaactc
    11821 tataaccaac tgaaagtatt gcatcaaggt tataaaaatt ggtgagatag tttttcccat
    11881 ctttggcagt tagttccatt ttggaactaa ctgaattttc actcaattct ccggaatcaa
    11941 aaatattttt tagatgtttt gtaatggcct gtctgcgagt tccaaacaat tctgcaatct
    12001 gagcttgcgt gagccaaatg gtttcatttt ccagtatcac ttcgagctca acagcgttat
    12061 cagactgata aagaattatt tcgttgttca ttttttgaaa tttttattat tgaattcatt
    12121 acctaatgag atattctatt ggtaaatttt cttcaatata tcctgcaagt ccagattgat
    12181 ttttgaaaaa gcaaaccact ttttacccag atctttcagc gaagctccaa tgtgaaagat
    12241 ggtttcgtcg ataatcaaaa atcgatcatg tgatttggaa aattccttca cttcgagagg
    12301 tggatattga gcattaaatt tttccaaatc cagtttgaat tgcgctgaaa ttttattggt
    12361 aaaaatttga catttaacat tttcctttct ttttgaaagc aaaatcagaa cgctttcatc
    12421 caaataattg tcaatcaaaa cgattgattt tgttgccgat ttcaccaaat ctgcaacaaa
    12481 cttgtaggca tcaaaaatct gtccatcgta aaaaattccc tgcaccggag gaagagctgt
    12541 tttaacaaag aaatcgattt tatcctctgt tgccgaaacg cgtctttcca aattttcaat
    12601 acgttggttg accgcataac ctttcaacaa ataatctttc aaaaccttgt ttgcccatat
    12661 cctgaattga gtaccacgtt tggattttac acgataacct acagatatga ttacatccag
    12721 attatagtat tcaatcaaat atgtttttcc atccgaggca gttgttgcat tttttgcaac
    12781 aactgaattt ttatcaagct cttgttcttt aaaaatatta cttatgtgtc ttgaaataac
    12841 tgatttatct ctttcaaaca atatactcat ctgattcaga gtgagccaca cggtttcatt
    12901 ttccaacatt acttcaagct gaacagcatt gtcggactga taaagaatta tttcgttgtt
    12961 ggcattcatt cgctaagcag tcaaattttg atacagttcc atcaaatttt ccattcaaat
    13021 ccatctttgg tgtcctttat ttcaaagccc aaagcagtca attcgttgcg gattttgtcc
    13081 gaagtagccc aatccttatt cctttttgct tccagtcgaa tttgcagcaa taaatcaacg
    13141 gcttttttta gtgcttcgta tttggcaaaa ttttcatctt cttttttaag tcccaacaag
    13201 tcttccacaa acactcgaaa aacgtctttc agttcttcca aatcggcaga cgaaacagta
    13261 gcttttccat cataaaccag attgataaat ttcactgctt caaacaaatg tgcaataaca
    13321 ataggagtgt tcaggtcatc acacatggct tcttcacagc gctgtcgaag tccacttata
    13381 tcggaggttg aaatatcgga tggcatcaac ttttgcaatc tatcatatcc ttccattaag
    13441 cgttccaatc ctttttcaga ggcttgtaat gcttcattgc tgaaatcgac cgtactgcga
    13501 taatgtgcct gcagaataaa aaaacgaacc gtcatgggct gatacgcttg agaaagcaaa
    13561 ggatggtttc cggagaaaaa ttcttccaaa gtaatgaagt tccctaatga ttttcccatt
    13621 ttttgaccgt tgatggtaat catgttgttg tgcatccaat aacgaacact ttccttaccg
    13681 agtgcagcag ttgactgcga aatttcgcat tcgtgatggg gaaaaagcaa atccatgcct
    13741 ccgccatgaa tatcaaattc ttcacccaaa tatttggtac tcatcgccga gcattccaga
    13801 tgccagcccg gaaaaccttc gctccagggc gatttccagc gcatgatatg ttcaggggat
    13861 gcttttttcc ataaagcaaa atccacgctg cgccgttttt cttcctgtcc ttcaagcata
    13921 cgcgttgttt ccagcagttc gtctatattt ctgcccgaaa gtttaccgta atgatatttc
    13981 ttatcgtatt tttcaacatc gaaatatacc gatccttcac tttcataggc gaatcccgca
    14041 tcgagaattt tttttacaaa ttcaatttgt tcagggatat gactcgaagc atgaggctcg
    14101 atacttgggg acagaacatt caaagcttcc atagctttat ggtaacggtt ggcataatat
    14161 tgagcaattt ccattggttc caactgttcc agacgtgctt ttttttcaat tttatcttcg
    14221 ccactgtcgg catcattttc caaatgaccc acgtcggtaa tatttctcac atatcgaacc
    14281 ttataaccca aatgtttcag gtagcgaaac aaaacatcaa atgttatggc cggtcgtgca
    14341 tgtcccaaat gtggatcgcc gtaaacggtt ggtccgcaaa cgtacattcc cacatgcggt
    14401 gcatgaagtg gaacaaacag ttcctttttt ctgtgtaaag tattgtaaat gtataagtta
    14461 ttttccattt ggttataaaa atttttttgg atttttattg ataacttgct tttttcaggt
    14521 aaattttctt tcctttttta aagtttcgta agcctcgttt atggatcgga atttttctgt
    14581 ggcctttttc ttcacatctt ctcccaatgt gttgaccttg tcggggtgat atttcatggc
    14641 cattttccgg taagctttct taatttcatc cacagaagcg gaaggttcaa tttcaagagc
    14701 tttataggcc caattgatgt caacttgttt tatataaggt gcttttaaag attcaaaatc
    14761 cagtccggaa atacgaaata tgccagcaat tctttcaatc aatgtcagtt cggaagtaga
    14821 aacctgatga tcggcatatg caatgtcgaa aagcaaatga atcagttcca gccgtgtaga
    14881 atagtttgta taacgataaa tctgttctgc aacctgacgt tcgtcaatat ctttttcaag
    14941 cagatttttt aatattgata atgcttccaa tgctccctct tctccaaaat tatcaaccaa
    15001 aaaacgtttg actatttcca attccgattt cttgacgtgt ccatcagctt tcatcataca
    15061 agcaatcaaa accaacagac ttattttgaa atctcctgaa gttgtggtag ttcttctctt
    15121 ttgagatttc tctactgttt caattttagc gccaatgaat gagccgatgg caaaaccaat
    15181 caaagcaccg attggaccac ccaaagccca gcctaagcct cctccaatcc atttggcaaa
    15241 accactcata aaaacaattg gttacacatt tcagaaacca caaagatacg aaaaaagata
    15301 gaaatttaca ttcgtgtaaa attgagctga caacggctta aaaggagaat gattggttgt
    15361 tgaatttata tatctattca atttaatttt ttttaatttg gttttcataa aaatcaaaaa
    15421 taatcttgta aatttgcatg ctacaagtta ttctggttgt ttaactttat gcttttaaga
    15481 aattcaatca acactaaaat attttatcaa caaatttaat aaaacaagta atcatgaaat
    15541 taagcaaatt tattctcgca tttcttgttc tttcagcatt agtatttgcc ttgcctgctt
    15601 gcaaatccaa acagaaagta gttgaaattc ccggtgccaa tgcaaaagca ggtacttcga
    15661 cttcagaaaa tcaacaaaaa accacctcgc ctgtaatagt aacgcagcca acaacaagta
    15721 ctgaaaatgt ccccgaaatt acaagaagcg aaagtttcag tttggctgag ggtgaaacca
    15781 atcaaagcgc tatgaattat aaatatcatg tggtagtagg aagtttcaga aatcaatcaa
    15841 atgccaaagg actacaacgt acgcttagca atgaaggcta taatccagtt attgtaatca
    15901 acgaacaagg aatgtttcgt gtaattatga attcttttaa tacttacaat gaagcacacg
    15961 ttttcatcag taaaatacaa agccgattcc ctgatgcttg ggttttaatt aaaaaataaa
    16021 aagggatgtg tgtttcaaag ccatgaaata acgacttggt ttgactttcc tattaatttg
    16081 gctaatgctt aatctgggat aagttcttct tttggaatgt ttcatggctt ttggctcaac
    16141 gcaaaagaga agtttgtgca ttctcaacct taaactggct atttttgtct ttaatatatc
    16201 atttttaata ctgctctttt ttcttggttt tacttttttt attaggtatt ggtttttatt
    16261 tttattttgt gaattttttg aaagttccta tttccctttt gcttcgtgcc agccacttaa
    16321 aaaataagat gcttgttgat tgacattcca aattcctgat tctctttcct agcataagca
    16381 ccattggcaa aacatttttc tacagaatgg tcaaatattt tttctctttg aaaaatgctt
    16441 tccttgagga acttattgtt tgcggtagaa gctatttcta catatacatc aatttacttc
    16501 cttcccgttt tttcaaacca aaatagtcaa ctttttatgg gatgattaaa tggagtgaga
    16561 acttattttt tcttcggcgc aagcactact tttttctgac ttattctata ctgttgtata
    16621 aaaattaaca aaattaattc ggtacaggct caataattca atattaattt gataaaaaga
    16681 aaaatagaga atcgaaccaa agtcgtttaa aattgttgga taaagaaaaa acttaaaaaa
    16741 acaccaatca ttatggcaac acacataact aaaaccattt ggagaaaaga taatattttc
    16801 gataccgatg ttgacgggca catcataacc atagatttag gcaaggaagc cggaggagaa
    16861 gatgctgggc cacgtcccaa aaaactgctg ttggtggcag cggcaggttg tagtggactg
    16921 gatgtagttt cgcttttgaa aaaaatgcgt gtggaactga aagggtttga tattcagata
    16981 gaagcgcaaa tgagtgaaga gcatcccaaa caatacacat ccatgaaagt aatctacgaa
    17041 tttgaaggag acaatttacc gatggaaaaa ctggaaaaag ctgttaccct ttcctacgaa
    17101 aaatattgtg gtgtcatggc catgtacaaa aaagctattc ctatttcatg ggaaataaga
    17161 atcaaatcct gagcaagctt aaaacttttc ttttcccatt tgttaaataa accacgaaat
    17221 tttgctaaat ttgcaacagt tttctttttc attgaattta caaaataatc accactttaa
    17281 atatttgaag actatgcaaa caatggattc ttttaatttt gccggcaaaa aggcatttgt
    17341 tcgtgtggat tttaacgtgc cactggatga aaatttcaat attaccgacg atacgcgcat
    17401 tcgtgcagcc atcccaacac ttaaaaaaat tttgaatgat ggtggttgtg taattattgc
    17461 ttcacacatg ggaaggccca agaaaaatcc ggatcccaaa tattccttaa aatacattct
    17521 tcctcatgta tcaaagcttt tgggtataga ggttaaattt gcacccgatt gcataggacc
    17581 ggaaactgca gctatggttt cagctttaaa accgggagaa gctctaatgc tggaaaattt
    17641 gcgcttttat gaagaagaag aaggaaaacc gcgtttgccc gaaacggcca ccgaagaaga
    17701 aaaagctgct gcaaaaaaag aattgaagga acgtcagaaa aaatttacgg ctacgctagc
    17761 ttcttatgcc gattgctacg tgaatgatgc ctttggaacc gctcaccgtg cccatgcttc
    17821 taccgcactc attgctgatt attttgatgc cgatcataaa atgttcggat atttaatgga
    17881 aaaggaagtg gcagctgttg aaaaaataat gaacaacacc gaacatcctt ttacagccat
    17941 catgggaggc tcaaaagttt cttcaaaaat tgatattatt gaaaatctgc tcaataaagt
    18001 tgacaatctg attattggtg gtggtatgac ttacactttt gtaaaagctc tgggtggaaa
    18061 aataggcagt tcaatttgcg aagaagacaa attggatttg gcactttcat tgctcgaaaa
    18121 agcaaaaagc aaaggggtaa atatggtatt ggccgttgat gcaaaaattg ctgacgcttt
    18181 cagcaacgat gcgaatacca aatttgttcc cgtaaacgaa attcctgaca attgggaagg
    18241 tttggacatt ggccccgaaa cagagaaact ttttgctgat gtaataaaat cctcaaaaac
    18301 cattttatgg aatggccccg ttggtgtttt tgaattcgat aatttcacgc acggatcttt
    18361 ggctgtagct caagctattg ttgaagccac caaaaatggt gcattttcgc tcattggtgg
    18421 aggcgattct gttgcctgca tcaacaaatt cggtttggca gatggcgttt cttatgtttc
    18481 tacaggtggc ggagctttac tggaagccat tgaaggaaaa gttcttcccg gtatcaaagc
    18541 cattagaggc tattaatttt caactttgat tttcatattt taaatagagg gtgtcccaaa
    18601 aggacacttt cttttttata ttaagcagcc cgtttaaggg
//
LOCUS       DAOSCH010000030        18336 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2115_length_18336_cov_5.962584, whole genome shotgun sequence.
ACCESSION   DAOSCH010000030 DAOSCH010000000
VERSION     DAOSCH010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18336
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2115_length_18336_cov_5.962584"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..1405
                     /locus_tag="PLZ54_03850"
     CDS             275..1405
                     /locus_tag="PLZ54_03850"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ66102.1"
                     /translation="MAITVREVTTKKQLKQFVWFGINLYKDNEYAAPPLFIDDYTNLT
                     KGKNPSLDFCEAAYFLAYNEKNEIVGRIAAIINPVANETWNQKNARFGWVDFIDDYEV
                     SKALFDAAVEWARAKGMTAMQGPLGFTDFDHEGTLIEGFDRLGTLAGIYNYPYYSQHI
                     EKYGFVKDADWVEFLITIPEKFPDKYFRIAELVKQKYHLTPRHFKSRKKLVNGYGNKI
                     FDLLNTCYQHLYGFTKLNEAQKQFYIKLYFSFFRLDTVSVVTDENDDVVAVGIAMPSL
                     TKALQKAKGKLFPLGWFYLLKSIYYKNDIVDLYLIAVHPRYQGKGVNSILFAELMVNF
                     AKNGYKFAESNPELETNTQVSSQWSDFEHINHKRRRAYIKQI"
     gene            1570..3156
                     /gene="nadB"
                     /locus_tag="PLZ54_03855"
     CDS             1570..3156
                     /gene="nadB"
                     /locus_tag="PLZ54_03855"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOJ66103.1"
                     /translation="MTRKFDFLVIGGGIAGISYALKVAEYGKVALICKTTLEESNTSL
                     AQGGMAAVLYPPDNFEKHIQDTLIAGDYINNIEAVKKVVYEAPKQIEELIRWGVKFDK
                     AEDGKFDLHREGGHSEFRILHHKDNTGAEVQKRLNKKVLHHPNIEIFEYHFALEILTQ
                     HHLGQIVTHHTPNIECYGAYILDQKNNQIITFLSKVTMLATGGIGGAYLTSTNPPTAT
                     GDGIAMVHRARGVIKDMEFVQFHPTALYHPGDKPSFLISEAIRGYGAILRTKDGQKFM
                     HKYDERKELAPRDIVARAIDNEMKIRGDEYVYLDVTHKDPKETKKHFPTIYKRCRELG
                     INITKDYIPVAPTQHYLCGGIAVDLNAESTIHRLYAAGECSCTGLHGANRLASNSLIE
                     AIVYADAAAKHSVVRLNSLGFQENIPDWDDEGTTWPEEMILITQSAREVEQIMNNYVG
                     IVRSNLRLKRAMSRLEILFRETEDLYERSVVSRQICELRNIISVSYLIIKQATQRKES
                     RGLHYNIDYPGHAEDLKNKE"
     gene            3301..3690
                     /locus_tag="PLZ54_03860"
     CDS             3301..3690
                     /locus_tag="PLZ54_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ66104.1"
                     /translation="MKRTMLIVFWLALATMLLAGSYSPDDPRSSAQQVVVKSDVAVGQ
                     NWLRQDNSSGASDNQNQNIDNVSFSATSEGIYLSLGSGTYNVKLFALTGQLLWSGDLV
                     QGKFFIPVRKGIYFLRINGKSFKVVCK"
     gene            3762..4067
                     /locus_tag="PLZ54_03865"
     CDS             3762..4067
                     /locus_tag="PLZ54_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HOJ66105.1"
                     /translation="MVKHIVMFQLSDFSEGKTKKENATLIKSQLENLQHLIPELKKIE
                     VGIQMEETAAENYDLVLYTEFENVEDLQIYINHPEHKKVSAYIGKIKTGRVAVDYKV"
     gene            complement(4159..6618)
                     /gene="pheT"
                     /locus_tag="PLZ54_03870"
     CDS             complement(4159..6618)
                     /gene="pheT"
                     /locus_tag="PLZ54_03870"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOJ66106.1"
                     /translation="MNISYNWLKKYINIDLPVEKVSEILTSIGLEVGSTEEVQTVKGG
                     LEGLVIGEVLTCVPHENSDHLHVTTVNIGSGEPLQIVCGAPNVASGQKVVVATIGTKL
                     YAGDESFTIKRSKIRGVESFGMICAEDEIGIGTSHEGIIVLPDDAKPGMLAKDYYGIK
                     SDYVMEIDLTPNRVDAASHFGVARDLAAYFSLRDKSIKLTLPSVDNFQVQQHGLTIPV
                     TVENPEACPRYSSVTITGVKVGESPKWLKNYLTTIGLRPINNVVDATNFVLYELGQPL
                     HSFDADKIKGGKVIVRHMPEGTTFVTLDGVERKLSAEDLMICNAEEPMCIGGVFGGLD
                     SGVTESTQNVFLESAYFNPVYIRKTARRHGLSTDASFRFERGCDPNNTIYALKRCALL
                     IQEIAGGEIAGEIVDVYPKKILPFEVDITIAKINALIGKNFEKDTIQTILSALEIEIK
                     SSSEEGFVLKVPTYRVDVQRDVDVIEDILRIYGYNNVEIGEKLVSTLSYRPKPNNFRI
                     QNIISEQLTAQGFNEVMNNSLTKRNYYALSATLPLEKCVKIMNPLSSDLSVMRQTLLF
                     GGLENIVYNNNRKNFDIKFYEFGNCYFYLPENKNSSDPLSAYLEEFHLGLWLSGNKHP
                     QSWTSSEEKSSIFELKAYVENIFRRLGLKLQHFHFEEISNDLFSQALSFRNAAGKQLA
                     LYGIVNAALLKSFDIENEVFYADIDWNLLIEETKGKQVSFAEISKFPEVKRDLALLLD
                     KSVTFAEIEKIAYQTERKLLKKVQLFDVYEGKNIEAGKKSYAVSFLLQDETKTLTDSQ
                     IDAIMQKLLKNFTEKLGAKLR"
     gene            6957..7029
                     /locus_tag="PLZ54_03875"
     tRNA            6957..7029
                     /locus_tag="PLZ54_03875"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6990..6992,aa:Gly,seq:gcc)
     gene            7065..7148
                     /locus_tag="PLZ54_03880"
     tRNA            7065..7148
                     /locus_tag="PLZ54_03880"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7099..7101,aa:Leu,seq:cag)
     gene            7173..7256
                     /locus_tag="PLZ54_03885"
     tRNA            7173..7256
                     /locus_tag="PLZ54_03885"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7207..7209,aa:Leu,seq:gag)
     gene            complement(7274..8191)
                     /locus_tag="PLZ54_03890"
     CDS             complement(7274..8191)
                     /locus_tag="PLZ54_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOJ66107.1"
                     /translation="MKNEDRTSQAMHATWMGFWVNFTLTVFKLLAGFLGKSTAMIADG
                     VHSLSDFITDLIVIIFVRIAGKEKDQDHEYGHGKFETFATFLVSLALAAVGVYIFING
                     LNKVIQVTQGEIIEPPTIIALIAALVSIIVKEGLYRYTARIGKKINNQAIVANAWHHR
                     SDALSSIGTGLGIAGAIFFGEQARILDPIAGIIVSFFIIGVALKILLPSVNELLERSL
                     PEETKKEIFEIIESNPEILSYHNLKTRKVGNTFAIDIHIKLDKTTTFLRSHDIATEIE
                     NKLRNRFGKQTIINIHTEPFNEEKDQKRR"
     gene            8744..9958
                     /locus_tag="PLZ54_03895"
     CDS             8744..9958
                     /locus_tag="PLZ54_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOJ66108.1"
                     /translation="MFRNQKITDDIYYVGVNDRQKAKFENLIPLYDGVSYNAYLIVDE
                     KTALIDTVDIHFGEILIQKILSQLGDKGLDYLIINHMEPDHSGSLRLLRKYFPNMTIV
                     GNERTLSMVKGFYEIDGSLLKVSDEESLSLGRHQLQFFLTPMVHWPETMMTFDHADKV
                     LFSGDAFGSFGALNGSILDENLDIDKYWDEMYRYYANVIGKYSSPVQKAIKKLEHLPY
                     EIICSTHGPVWKINLKKAVEIYDRLSSFQAEEGVVIVYGSMYGNTELMAETVAQGLAE
                     AGVKPVIIHNISTVDLSIILRDIYRYRGCIFGSPTYNNGLFPEMEDLIRKIESRGLKN
                     RIFGCFGSYTWSGAAVRNLTEFANRLQWPMSDVMVEEKQGLKKDNYESCLQLGHQIAE
                     MLQNPVQFNKGC"
     gene            10031..10257
                     /locus_tag="PLZ54_03900"
                     /pseudo
     CDS             10031..10257
                     /locus_tag="PLZ54_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122798.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
     gene            10260..11144
                     /locus_tag="PLZ54_03905"
     CDS             10260..11144
                     /locus_tag="PLZ54_03905"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOJ66109.1"
                     /translation="MTFWQTIIIAIVEGLTEFLPVSSTGHMIITQSFLGIESTEFVKA
                     FTVIIQFGAILSVIVLYWNRFFRLQPLDEFNVPTYSEGKKFVKQRVIIRKFFYRYDFY
                     WKLLIGFLPAAVIGFLAGDFIDVLLGDVFVVALMLFIGGFVMLFVDKWFSASSQDQSI
                     GWQRALKIGFFQCIAMIPGVSRSMATIVGGMAVKLTRKNAAEFSFFLAVPTMAAASGY
                     KLFQLLSEQGGKDLLVQNWLLLLLGNVIAFIVALVAIRFFIDFISRYGFKAFGYYRIA
                     VGGVILLLLGMGYNLNMM"
     gene            11145..11843
                     /gene="truB"
                     /locus_tag="PLZ54_03910"
     CDS             11145..11843
                     /gene="truB"
                     /locus_tag="PLZ54_03910"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOJ66110.1"
                     /translation="MDVFKGEVFYVDKPLHLTSFNVVNRLRWKIKKVAGIKKIKVGHA
                     GTLDPEATGVMIICTGKATKQIESFQSQTKEYIAELKLGATTPSFDMELPVNAVYPTE
                     HITRELIDQVVSTFIGEIEQIPPIFSAVKIDGKRAYEYARNGQEIELKPKKLVIDEIE
                     ILNFVPPTLTLRIVCSKGTYIRALARDIGFALNSGSFLTSLRRIRIGNVRIDDCWDIE
                     NLLNHLDKELKSTE"
     gene            11905..12954
                     /gene="queA"
                     /locus_tag="PLZ54_03915"
     CDS             11905..12954
                     /gene="queA"
                     /locus_tag="PLZ54_03915"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOJ66111.1"
                     /translation="MKLSQFKFKLPDELIAQYPPKHRDEARLMVVHKKTGEIEHRIFK
                     DVLDYFNENDLFIFNDTMVFPARLYGNKEKTGAQIEVFLLRELNAEMRLWDVLVEPAR
                     KIRIGNKLYFGEDDSIVAEVIDNTTSRGRTLRFLFDGTHEEFKKALYALGETPLPHYI
                     KRQVEAADAENFQTIFAKNEGAVAVPAAGLHFSRELLKRMEIKGIDMAFVTSHMSLGI
                     FRDIDVEDLTKHKMDSEQIIVPEETAKKVNKAQEEGRNICAVGVTVMRALETVAGTEG
                     KIKSFDGWTNKFIFPPYDFSVANAMITNFQFPYSTLLMLTAAFGGYDTIMHAYEVAIE
                     EKYRFGVYGDAMLIV"
     gene            13137..14336
                     /locus_tag="PLZ54_03920"
     CDS             13137..14336
                     /locus_tag="PLZ54_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOJ66112.1"
                     /translation="MPHTSQRGQLMPESPIRKLVPLADKAKARGVKVYHLNIGQPDLK
                     TPQVALDTLKNIDLKILEYSPSDGFKSLRSKLAGYYDKFNIEATEEDIIITTGGSEAV
                     NFAFMSCLDPGDEIIVPEPAYANYTAFAIAAGVVVRPVVASIEDGFALPPIEKFEELI
                     TPKTKGILICNPNNPTGYLYTRAEMNKIRDLVKKYDLYLFSDEVYREFCYTGGPYISA
                     FHLDGIDENVILVDSFSKRYSECGIRVGALVTKNKEVRKNVMKFCQARLSPPLIGQII
                     AEASIDTPAEYMLEMYNEYLERRKFLIDGLNRISGVYSPIPMGAFYTVARLPVDDADK
                     FCAWLLSDFDYEGSTVMLAPGSGFYTIEGMGKNEVRIAYVLEKEDLKKALVVLEKALQ
                     AYPGKTI"
     gene            14419..15660
                     /locus_tag="PLZ54_03925"
     CDS             14419..15660
                     /locus_tag="PLZ54_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ66113.1"
                     /translation="MNVEYFIAKRIHFQQGKKNVSRPAVRIATIGIALGLAVMLISVC
                     VVIGFKSQIENKIIGFGGHIQITNFDNNNTYEMNPIKMDAALIQKIRQISGVVHVQRF
                     ATKPGIIKTDNEFQGIILKGIDKDFDWNFFRSNLLEGNILNVGSDTLKNEILLSKKLS
                     DLLGLKLGDELFMYFIQDQVRARKFKIVGIYSTNFVDYDQLFVLTDIRQVQRLNNWDS
                     ASFGGLEVLIDNFDKIDERGEAVYEATANIFSKDGNAYYTQTIKQLNPQIFSWLDLLD
                     MNVWVILLLMLSVAGFNMISGLLILILERTNMIGILKSLGATNWSVRKIFLYHSFFLI
                     AKGMFWGNLIGLSICALQYFTGIIPLDPEAYYVASVPVAFPWGYIVLLNIGTFVASIL
                     MMIAPSYLITKISPAKIIRYE"
     gene            complement(15839..16903)
                     /gene="corA"
                     /locus_tag="PLZ54_03930"
     CDS             complement(15839..16903)
                     /gene="corA"
                     /locus_tag="PLZ54_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOJ66114.1"
                     /translation="MAKYIKKRKKDIGVSPFELIFRGEKKMEKPRLYLMTYDENSVVE
                     TEIKSLRELNFNLNKDKFHWLNVEGLHEVKLMEKIAESFDIPFNIMSNIMDPMTRPQV
                     QDYEKGLFIVIKMLTYNEKLDKLDVENLSFILTKNTLILFQENQGDAFDPIRERLRKH
                     HHRIRTAGIDYLACALLDVVIDNYLYLIGQLGDKIEDVEELMTKELDNDIPSLINRYK
                     RELNYLRKYIMPAKEMILNLSKLESEFIRDDNHPYFKELQVNLNEVSELMDSYREILY
                     DQLSIYHNTLSSRLNDIIKILTIFSVLFIPLTFIVGVYGTNFDYFPEIHWQYGYFAMW
                     AVMLLIAGVMLWYFKKKKWL"
     gene            17169..17450
                     /locus_tag="PLZ54_03935"
     CDS             17169..17450
                     /locus_tag="PLZ54_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOJ66115.1"
                     /translation="MNLDVSVIGYLAGFCTAVAQFPQAYKVYRTKDTRSISLGMYAIM
                     TFGVFLWLIYGILIQDWPMMLANGIGLIPCTFNLIITFQNVFRERKKMI"
     gene            17528..17950
                     /gene="aroQ"
                     /locus_tag="PLZ54_03940"
     CDS             17528..17950
                     /gene="aroQ"
                     /locus_tag="PLZ54_03940"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOJ66116.1"
                     /translation="MKHIQIINGPNLNLLGEREPSIYGNKSFNGFFEELKKQYPDVKL
                     EYFQSNNEGEIIDKLQEIGFSADGIILNAGAYTHTSIAIADAIRSIQSPVIEVHISNV
                     FQRESFRHHSFLSEVCKGCIIGFGLNSYRLALEALLLI"
     gene            17999..>18336
                     /locus_tag="PLZ54_03945"
     CDS             17999..>18336
                     /locus_tag="PLZ54_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008654373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOJ66117.1"
                     /translation="MPKFTKIIATISDKRCDPDFIRDLFEAGMNVVRMNSAHLNREGF
                     LKIINSVRSVSNEIALLIDTKGPEVRTTIAENDKIILHTGDTIKIIGDPDKISTKECV
                     AVSYPFFVRD"
BASE COUNT         5610 a         3238 c         3554 g         5934 t
ORIGIN      
        1 tgtgttgtgg ttatattttt cttttatcgt tttatttacc ttaataagga aaaatattaa
       61 ctgaaaatca attatttaat aaaaaatgat tcaaaaaagt tcaaaattga ttctttcccg
      121 ttgaaaattg actaatttta catttttttt taacagataa aaagtacttt ctaaaaagca
      181 gtattactgt aatttcttat ttttggcaaa gaatgaaaaa agacattctg atgaaaaagc
      241 aaataaaata ttttttttaa aaacctaaac ggatatggcg ataactgttc gagaagtaac
      301 taccaaaaaa caattgaaac aatttgtctg gtttgggatc aatttgtaca aggataatga
      361 atatgcagct ccaccgctgt ttatagacga ctataccaac ctgactaaag gaaaaaaccc
      421 ttcgcttgat ttttgcgaag ctgcttattt tcttgcctac aacgaaaaaa atgaaattgt
      481 tggtcgcatt gcagccataa tcaatccggt tgccaatgaa acatggaatc agaaaaatgc
      541 acgtttcggg tgggtggatt ttatcgacga ctatgaagtt tcgaaagccc tgtttgatgc
      601 ggcagtggaa tgggcaagag ccaaaggcat gacagccatg caaggcccac tcggatttac
      661 cgattttgac cacgaaggta cgcttatcga aggttttgac cgacttggaa cattggccgg
      721 catatataat tatccttatt attcacagca catcgaaaaa tatggttttg tgaaagatgc
      781 cgattgggtt gaatttctca ttaccattcc cgaaaaattt cccgataaat atttccgtat
      841 cgctgagctg gtgaaacaaa aatatcactt aacacctcga cattttaaaa gccgaaaaaa
      901 actggtaaac ggctatggaa acaaaatttt tgatttgctc aatacttgct accagcattt
      961 atacggtttc accaaattga acgaagccca aaagcaattt tacataaaac tctatttcag
     1021 ctttttcagg ctggatacgg tttcggtggt aaccgacgaa aatgatgatg tagtagcagt
     1081 gggtattgcc atgccaagtc tgaccaaagc tttgcaaaaa gcaaaaggga aactattccc
     1141 gctgggttgg ttttatttgc tgaaatctat ttattataaa aacgatatcg tggatttgta
     1201 tctgatagca gtacatcctc gttatcaggg aaaaggtgtc aattctatcc tttttgccga
     1261 attgatggtc aattttgcaa aaaatggata taaatttgca gaaagtaatc cggagttgga
     1321 aaccaatact caggtttcct ctcagtggag tgattttgaa catatcaatc acaagcgcag
     1381 aagagcttac attaaacaaa tctgaaaatt tactttttct tgaaaaaatt atttaattcg
     1441 tttttttgac caactatgaa attttttgaa tggttggact ccacctatca acttctttca
     1501 acttttttag ctcgaatttt gtttgaatag tgtgaagtca ataaacaatt gacttaatac
     1561 atattgatta tgacacgaaa atttgatttt ttggtaatag gaggtggaat agctggcatc
     1621 agttatgcac tcaaagtagc tgaatacgga aaagttgccc tcatttgtaa aaccacactt
     1681 gaagaatcga atacttcttt ggctcaggga ggtatggcag ccgttcttta tccacccgac
     1741 aattttgaaa aacatattca ggatacttta attgcaggtg attacataaa taatatcgaa
     1801 gctgtaaaaa aagttgttta cgaagccccc aaacaaattg aagaattgat tcgctggggt
     1861 gtgaaattcg ataaggcgga agacggaaaa ttcgatttgc atcgcgaagg cggtcattcg
     1921 gaatttcgta ttctccacca taaggacaat acaggagcgg aagttcaaaa aagattgaac
     1981 aaaaaagttc tacaccatcc caatatcgaa atttttgaat atcattttgc tctcgaaatt
     2041 ttaactcagc atcatttggg tcaaattgtt acgcaccata ctccaaatat cgaatgttat
     2101 ggcgcttaca ttcttgacca aaaaaacaat caaattatta cttttctttc aaaggttacc
     2161 atgttggcaa ccggaggtat tggaggtgca tatctgactt ctaccaatcc gcccactgcc
     2221 actggcgatg gcattgcaat ggtacacaga gccagaggcg taataaaaga catggaattt
     2281 gtgcagtttc atcccactgc actttaccat ccgggtgata aaccctcttt cctgatttcg
     2341 gaagccatac gtggctatgg agcgattttg cgtaccaaag acggacaaaa attcatgcac
     2401 aagtatgacg aaagaaaaga gctggctccc cgagacattg tagcccgcgc tatcgacaac
     2461 gaaatgaaaa tacgcggtga cgaatatgtt tatttggatg ttacgcacaa agacccaaaa
     2521 gaaacaaaaa aacatttccc caccatttat aaaagatgcc gcgagttggg tatcaacatt
     2581 accaaagatt atattcccgt agctccaaca cagcattatt tgtgtggtgg cattgctgtg
     2641 gatttgaacg ccgaatcaac cattcatcgt ctgtatgcgg caggagaatg ttcctgtacc
     2701 ggtttacatg gagccaaccg tttggcttcc aattctttga tcgaggcaat tgtttatgct
     2761 gatgctgcag ccaagcattc tgttgtccga cttaattcac ttggttttca ggaaaatatt
     2821 cccgattggg atgatgaagg cacaacttgg cctgaagaaa tgattttgat tactcaaagt
     2881 gcgcgggaag tagaacaaat catgaataac tatgtaggaa ttgttcgttc caatttgcgt
     2941 ttgaagcggg ctatgagccg attggaaatt cttttcaggg aaacggagga tttatatgag
     3001 cgatcggtgg tttcgcgcca aatttgcgaa cttcgaaaca ttatcagcgt ttcgtacttg
     3061 attatcaagc aggctacaca gcgaaaagaa agtcgcggat tgcattacaa catcgattat
     3121 ccgggacatg cagaagattt aaaaaataag gaataacgac ctgaatattt ttcgataacg
     3181 aatcacaaaa taaactgaaa gtttatgaac cggcgataaa ttttttccct tttttctttg
     3241 gacgtttttc actatttttg cagtaaatta tttctcaacc tgttaaattt tctatttgcg
     3301 atgaaacgta cgatgttgat tgtgttttgg cttgctttgg ccacgatgct tttagccgga
     3361 agctattctc ccgacgatcc gcgttcatca gcccaacaag tcgttgttaa atctgatgtt
     3421 gctgtgggac aaaactggtt gagacaggat aactcttccg gtgcatctga caatcaaaat
     3481 caaaatatcg acaatgtaag ttttagcgct acatccgaag gaatttattt gtcattgggc
     3541 agtggaacat ataatgtgaa actttttgca ctcaccggac agttgttgtg gagtggcgat
     3601 ttggttcagg ggaaattttt tattccggtg cgaaaaggaa tttatttttt aagaatcaac
     3661 gggaaaagtt ttaaagtggt ttgcaaataa aactaatact tttgccccca ttaaaagaaa
     3721 ccggaaaaaa ctctatccga taaattataa agaaattttt tatggttaaa cacatcgtta
     3781 tgtttcagct ttccgatttt tcggaaggaa agacaaaaaa agaaaatgca acgctcatca
     3841 agtcgcaatt ggaaaatctt caacacttga tacctgaatt gaaaaaaatc gaggtcggta
     3901 ttcaaatgga agaaacggct gcagaaaatt atgatttggt attgtacacc gaatttgaaa
     3961 atgtagagga tctgcaaatt tatatcaacc atcccgagca caagaaagtc agcgcatata
     4021 tcggtaaaat taaaaccggc agagtagcag tagactacaa agtttgatca aaaaaaaaca
     4081 ggaaaaagaa aagatttttc tttatcacaa gcaaaggtga aaagaaaaat ctttttttat
     4141 gtatcggttt gttctgcgtt aacggagttt agcacccagt ttttcggtga aatttttcag
     4201 aagtttttgc ataatggcat caatctgact gtcggtaagt gtttttgttt catcctgcaa
     4261 taaaaagctc actgcataag attttttacc tgcttcgata tttttgcctt cgtacacgtc
     4321 gaacaactga acttttttca aaagttttct ttcggtttga taggctattt tctcaatttc
     4381 ggcaaatgtt acgcttttat cgagcaacag ggccaaatca cgttttactt caggaaattt
     4441 tgatatttcg gcaaaactaa cctgtttccc tttcgtttct tctatcaaca aattccaatc
     4501 aatatcggca taaaaaactt cattttcaat atcaaaactt ttcagcagtg cagcattcac
     4561 tattccgtac aatgccagtt gtttaccggc tgcattgcga aagctcaaag cttgtgaaaa
     4621 caaatcgtta gaaatctcct cgaagtgaaa atgctgcaat ttcaatccca gcctacgaaa
     4681 aatattttcc acgtatgctt tcagttcaaa aatacttgat ttttcctccg aagaagtcca
     4741 cgattgcgga tgtttgttgc ccgacaacca aagtcccaaa tgaaattctt ccaaatatgc
     4801 cgataaagga tcggatgaat ttttattttc gggaagatag aaatagcaat tcccaaattc
     4861 ataaaacttt atgtcgaaat ttttccgatt gttgttgtaa acaatgtttt ccaaacctcc
     4921 aaaaagcaga gtttgtctca tcacgctcaa gtccgaactc aacgggttca ttatttttac
     4981 acatttttct aaaggcaaag ttgcagaaag tgcgtaataa tttcttttgg tcagcgaatt
     5041 gttcattact tcattaaaac cctgagctgt cagttgctcg gaaataatgt tctgaatacg
     5101 gaaattgttc ggtttaggtc gatagctcaa cgtggaaacc agtttttctc cgatttctac
     5161 attgttgtaa ccataaatac gaagaatatc ttcaatgaca tccacatcgc gttgcacatc
     5221 caccctgtag gtcgggactt tcaaaacaaa accttcttcg gaagaagatt ttatctcaat
     5281 ttccaaagcc gaaagaatgg tctgaatggt gtccttttca aaattttttc ctatcaaagc
     5341 gttgattttc gcaatggtga tgtccacctc gaaaggaagt atttttttgg ggtaaacatc
     5401 aacaatttct cctgcgattt ctcctccggc aatttcctga atcagcaaag cacagcgctt
     5461 caaagcgtaa atggtgttgt tggggtcgca gcctcgctca aagcggaaag aagcatcggt
     5521 actcaatccg tgccgacgag ccgtttttcg gatataaacg ggattgaaat aggcactttc
     5581 cagaaaaacg ttttgtgttg attctgtaac tcccgaatcg agtccaccaa aaacgcctcc
     5641 gatacacatg ggctcttctg cattgcaaat catcaaatct tcggcgctca atttacgctc
     5701 tactccatcc agcgttacaa aagtcgttcc ttcaggcatg tgtcgcacaa taacttttcc
     5761 gccttttatt ttatcggcat caaaagaatg aagcggctgc cccaattcat acaacacaaa
     5821 attggtggca tccaccacgt tgttgatggg acgcaagcca atggttgtca ggtaattttt
     5881 cagccatttg ggcgattcgc caacctttac gccggtaatg gttacagaag aatatcgcgg
     5941 acaagcttcc ggattttcta ctgttaccgg aatggtaagc ccatgttgtt gaacctgaaa
     6001 attatctacc gacggcaggg taagttttat cgatttgtct cttaacgaaa aataggcagc
     6061 caaatcacga gcaacgccga aatgtgaagc agcatccacg cggttgggtg tcagatctat
     6121 ttccatcaca taatcgcttt ttatgccgta ataatctttt gccaacattc cgggttttgc
     6181 atcgtcgggc aaaactatga tgccttcgtg actcgttcca attcctattt catcttcggc
     6241 acaaatcatt ccgaaagatt ccacaccgcg aattttggag cgttttatgg taaaactttc
     6301 gtcgccggca tacagttttg taccaatggt agccacaacc actttttgtc cggaagccac
     6361 attcggagcg ccgcaaacaa tctgcaaagg ttcaccggag ccaatattga cagttgttac
     6421 gtgcaaatga tcagaatttt catgaggaac gcacgtcagc acttctccaa tgaccaatcc
     6481 ttccaaccca cctttcacag tttgaacttc ttcggtgctg ccaacttcca atccgataga
     6541 agtcagaatt tccgaaacct tttctaccgg caaatcgatg ttgatgtatt ttttgagcca
     6601 attatacgag atattcatat ttgtttgttt ttatagtcaa ttatttggaa agggtcttga
     6661 tttttccgct gcaaaagtac atgaaaatcg aggtatttcc aacttccaac tataaaatct
     6721 tgaacaaaac cttaatagct tgccatagga taatagttga acagtttcta ttgataggtt
     6781 gcatttaatc aattacagaa ggatacagct acggaatata gaaagaaaaa agtttttgaa
     6841 gaattagttc cttgtatttt tttaaaagaa aaatatcaag atttttcctt tgaaattttt
     6901 tgcaaattag caaaaagaaa gtatctttgc tgcgcttttc caaaagatca aattgtgcga
     6961 aaatagctca gttggtagag cacgaccttg ccaaggtcgg ggtcgcgggt tcgagtcccg
     7021 tttttcgctc aaaacttatc cctgcaattt tgaaaacagg aatagcccaa atggcggaat
     7081 tggtagacgc gctagtttca gggactagtg gccgttaggc tgtgcaggtt cgagtcctgt
     7141 tttgggcaca ccaagttttc attttgtttt ttgcgcagat ggcggaattg gtagacgcgc
     7201 tactttgagg gggtagtgcc ggttacggcg tgtgagttcg agtctcattc tgcgcaccct
     7261 gagtaaatct tgtttatctt ctcttttggt ctttttcttc gttaaatggt tcggtatgaa
     7321 tattaatgat ggtttgttta ccaaatcgat ttcttagctt attttcaatt tcagttgcaa
     7381 tatcgtgaga acgtaaaaag gttgtagttt tgtcgagttt aatgtgtata tcgatggcaa
     7441 aagtgtttcc tacttttctg gttttgaggt tatggtaaga cagtatttca gggttcgatt
     7501 cgataatttc aaatatttcc tttttcgttt cttctggtaa tgacctttct aaaagttcat
     7561 tgacgctggg taataaaatt ttcagggcta ctcctattat aaaaaagctg acaatgatac
     7621 ccgctatcgg atctaaaatt cgagcctgct caccaaaaaa aattgctccg gcaataccta
     7681 atccggtacc tattgatgat aaggcatcgg agcgatggtg ccatgcattt gctacaatgg
     7741 cttgattgtt aatttttttc ccaattcttg ctgtatatcg gtataatcct tccttaacaa
     7801 taatactcac cagagctgct atcaatgcta taatggttgg tggttcaatt atttctccct
     7861 gtgttacctg tataactttg tttagaccgt ttatgaaaat gtaaactcct acagcagcga
     7921 gtgcaaggct gactagaaaa gttgcaaatg tttcaaattt tccgtgtccg tattcatggt
     7981 cttgatcttt ttcttttcca gcaattctta caaatataat aacaatcaaa tctgttataa
     8041 aatcggatag tgagtgtact ccatctgcaa tcatggcagt acttttaccc aaaaaacccg
     8101 ccaaaagttt aaatactgtt aaggtaaaat taacccaaaa acccatccac gtagcatgca
     8161 tggcttgtga tgttctgtct tcatttttca tatttcactt caatgataga aaaatattca
     8221 tgaatgcaaa aataataaat aataaatggt tttttcacct atttttgaag taacaagcta
     8281 atttataaga agtttaaata atattttaaa ctattctaaa ttactttatt ccagtcaaaa
     8341 ttcacttaaa agcagtaatc aaggaatcaa gtaagaaaat tctttttcaa cagaatataa
     8401 tttattttta aaggtctgat ggaacccaca tgtctgaata ttatattcat tcttgtttgt
     8461 gtatgcaaca catgtgggtt tcataaacaa ctctgtaaaa cctcatagct atttggtttt
     8521 ctacaaagtt aaaacttttt cttttaggaa tttttgtttt gatttaccaa aaatgagttt
     8581 ttcaaacatt ttatctaatt tcgcatttgt cggttgaatt ttcctcgtca taaggctgac
     8641 tctatatttt tcctatttgg agaatttatt ttatctttgt agttcgattt tttatttttg
     8701 aaatcactat cattacaaac tgagaagtta aaattgacta aaaatgttcc gtaaccaaaa
     8761 gataacagac gatatatatt atgtaggcgt taacgataga cagaaagcga aattcgagaa
     8821 tttaattccc ttatacgatg gtgtttctta caatgcatat ttgattgtag atgaaaaaac
     8881 cgctttgatt gatacggtag atattcattt tggcgaaata ttgatacaaa aaatactctc
     8941 acaattgggc gataaaggat tagactatct gattatcaat cacatggaac cagatcattc
     9001 gggttccttg cggttgttga gaaaatattt tccaaatatg actattgtag gcaatgaaag
     9061 aacattgagc atggtgaaag gattttatga aattgatgga tcgcttttga aagtttccga
     9121 tgaagaaagc ctttctttag ggcgccatca attacagttt tttctgactc cgatggttca
     9181 ttggccggaa acaatgatga cttttgacca tgccgataag gtgctttttt caggcgatgc
     9241 ttttggaagc tttggtgcac ttaatggaag tatattggat gaaaaccttg atatagacaa
     9301 atattgggat gaaatgtatc ggtattatgc taatgttatt ggtaaatata gttcgccggt
     9361 acaaaaagcc ataaaaaaat tggagcattt accttatgaa ataatttgca gcacacacgg
     9421 ccccgtgtgg aaaatcaatc taaaaaaagc tgtagaaata tatgatagac taagcagttt
     9481 ccaagcagaa gaaggagtag tgatagttta tggatcaatg tatggaaata cagaactaat
     9541 ggctgaaacg gtagcgcaag gtttggcaga agcaggtgtt aaacccgtga ttattcacaa
     9601 tatttcaaca gtcgatcttt ccatcatttt gcgagacatt taccgatata gaggttgtat
     9661 ttttgggagt ccaacttata acaacggact atttcctgaa atggaagatt taattagaaa
     9721 aattgaaagt cgtggattaa aaaacaggat attcggttgt tttggttcct atacgtggtc
     9781 gggcgcagca gtgagaaact tgactgaatt tgcaaatcgc ttgcaatggc ctatgtctga
     9841 tgtgatggtg gaagaaaaac agggtctaaa aaaagataat tatgaatctt gcctacaatt
     9901 aggtcatcaa atagcagaaa tgttacaaaa tcctgttcaa ttcaataagg gctgttaacc
     9961 gcgtaacttt ttaaaaaagg taatatgttt cttcctgtgt atattttaac ttactaaatt
    10021 ttttaacgta atggaaaata gacattcatc cggacttggc aaaataaatt tggtgcttat
    10081 agcatccgga tttgcaatta ttgttatagg ttttttctaa tgacaggttc ttctacccaa
    10141 gtggaattta atccggatat tttcagtttt cgtcgcataa ctgtaggccc aatggtttca
    10201 ttttttggtt ttttgtttat catatttgcc attcttttta aaccaaaaag taaataatga
    10261 tgactttttg gcagacaatt ataatcgcca ttgttgaagg actaacggaa tttcttcctg
    10321 tttcttcaac tggacacatg ataattacac aaagttttct gggtatagaa agtacggaat
    10381 ttgtaaaagc tttcactgtt attattcaat ttggagctat tctttctgtg attgttcttt
    10441 attggaacag attttttcgg ttgcaaccac tggatgaatt taatgtgccg acctattctg
    10501 aagggaaaaa atttgtgaaa caacgtgtta ttatccgaaa atttttttat cgatacgatt
    10561 tttactggaa actgttaatt ggttttttac ctgctgctgt cataggtttt ttagcaggcg
    10621 attttattga tgttttgctc ggagatgttt ttgttgttgc tttgatgctc tttataggag
    10681 gctttgtgat gctttttgta gataaatggt tttcagcctc ttctcaagac cagtcaattg
    10741 gttggcaaag agctttaaaa atcggttttt ttcaatgtat agccatgatt cctggagttt
    10801 cccgttcaat ggccaccata gtaggtggaa tggcggtaaa attaacgcga aagaatgcgg
    10861 cagaattttc gtttttcctt gctgttccta ctatggctgc agcttcaggg tacaaacttt
    10921 ttcagcttct cagcgaacag ggtggaaagg atttgctggt gcagaactgg ttgttgctgt
    10981 tattgggtaa tgtaatagct tttattgttg cattggttgc cattcgtttt tttatcgatt
    11041 ttataagtcg ttatggattc aaggcattcg gttattatcg catagcagta ggaggagtta
    11101 ttttattgtt gttgggaatg ggttataatt taaatatgat gtaaatggat gttttcaaag
    11161 gcgaagtttt ttatgtggat aaacctttgc acttgacatc attcaatgtt gttaataggt
    11221 tacgctggaa aattaaaaaa gttgccggaa taaaaaaaat taaagttggc catgccggca
    11281 cgcttgatcc ggaagcaacg ggcgtgatga ttatttgtac tggtaaggca accaaacaaa
    11341 ttgaaagttt tcagtcccaa accaaagaat atattgccga attaaaatta ggtgcaacta
    11401 caccatcctt cgatatggaa cttccagtaa atgcggttta tccaaccgaa catatcacgc
    11461 gagaattgat agatcaagtt gtgtcaacct ttattggtga aatagaacaa atccctccga
    11521 tattttcagc ggtgaaaata gatggaaaaa gagcttatga atatgcaaga aatggacagg
    11581 aaattgaact aaaaccaaaa aaattggtta tagatgagat tgaaatactc aattttgttc
    11641 ctcctacatt aacgcttcga attgtttgca gtaaaggaac ttatattagg gctttagctc
    11701 gcgatattgg ttttgctctt aactcgggat cctttcttac ttcattgaga cgcatacgca
    11761 ttggaaatgt tcgaatcgat gattgttggg acatcgaaaa tttgttgaac catttggata
    11821 aagaactaaa atcgacagaa taaaaattct tacattataa ggcttttgga acaactttaa
    11881 aaattaaatc tttagctgat aaatatgaaa ttatctcaat ttaaatttaa attacccgat
    11941 gaattgattg cccagtatcc tcctaaacat cgagatgagg cacgattaat ggtagttcac
    12001 aaaaaaaccg gagaaattga acatcgtatt tttaaagatg tgttggacta tttcaacgaa
    12061 aatgatctct ttattttcaa cgatacgatg gtttttcccg cccgtcttta tggaaataaa
    12121 gaaaaaacag gagctcaaat tgaggttttt ttgcttcgtg agttaaatgc tgaaatgcgt
    12181 ttatgggatg ttttggtaga acccgcccgt aaaattcgaa taggaaataa actttatttt
    12241 ggggaagatg attccattgt ggcggaagtt atcgataata ctacttcacg cggtcgaacc
    12301 ctacgttttt tgtttgatgg cacacacgaa gaatttaaaa aagcgcttta tgccttagga
    12361 gaaacacctc ttcctcatta tatcaagcga caagttgaag ctgccgatgc agaaaatttc
    12421 caaacaattt ttgccaaaaa tgaaggagca gttgccgttc ctgcggcagg tttgcatttt
    12481 agtagagaat tgttaaaaag gatggaaatt aagggaatag atatggcttt tgttacttct
    12541 cacatgagct tgggtatatt tcgagacatt gacgttgaag atttgaccaa gcataaaatg
    12601 gattccgagc aaattattgt tcctgaagaa acggccaaaa aagtcaacaa agctcaagag
    12661 gaaggaagaa atatctgtgc agtaggggtt acagtaatgc gagcactgga aactgtagcc
    12721 ggaacagaag gaaaaattaa atcctttgat ggctggacaa ataagtttat atttcctccc
    12781 tatgattttt cagttgctaa tgcaatgatt accaactttc agtttccata ttccactttg
    12841 ctcatgctaa cagctgcttt tggcggatat gataccatta tgcatgccta tgaggttgct
    12901 attgaagaaa aatatcgatt tggagtatac ggagatgcta tgttaattgt ttaattattg
    12961 acttcctact caatgtcata tttcattgac aggaaaattt tcaatacgat aaaaaaacaa
    13021 catttcattt ttatcgggta aattcattaa ttttggaaat cggttgaaat ggtaaatttc
    13081 atttattcag taccgaataa ttttatattt gttaatttgc aaatttaaaa caacacatgc
    13141 ctcacacttc acaacgtggg caactcatgc ccgaatctcc catccgcaag cttgttcctt
    13201 tggccgataa agccaaagca cgcggagtaa aagtttatca tttgaacatt ggccagcccg
    13261 atttgaaaac gccacaagtt gctttagaca ctttaaaaaa tatagatctt aaaattttgg
    13321 aatacagccc cagcgatggt tttaaaagtt tacgcagcaa attggccggt tattacgaca
    13381 agttcaacat tgaagcgacg gaagaagata ttattatcac taccggaggt tcggaggcgg
    13441 taaattttgc ctttatgagt tgtctcgatc cgggtgatga aatcattgta ccggagcctg
    13501 cttatgccaa ttacacggct tttgccattg ctgcgggagt ggtggtacgt ccggttgtgg
    13561 cctccattga agatggcttt gctctgccac ctattgagaa atttgaagaa ctgataactc
    13621 caaaaaccaa aggaatttta atttgcaatc ccaataatcc taccggctat ctttacacgc
    13681 gtgccgaaat gaataaaatt cgcgatctgg tgaagaaata cgatttgtat cttttttccg
    13741 acgaagttta tcgtgaattc tgttatacgg gtggtcctta tatttcggct tttcatctcg
    13801 atggcattga cgaaaatgtg atattggtcg attctttttc caaacgttac agtgaatgtg
    13861 gtatccgtgt tggtgcactg gtaacgaaaa acaaggaagt gagaaagaat gtgatgaaat
    13921 tttgtcaggc tcgtctcagc cctccgctta taggtcaaat tattgccgaa gcttctatcg
    13981 acacgcctgc tgaatacatg ctcgaaatgt acaatgaata tctcgaacgg agaaagtttt
    14041 tgatcgacgg attgaatcgt atttcgggag tttattctcc aattccgatg ggtgcttttt
    14101 atacagtcgc tcgcttaccg gttgatgatg ccgataaatt ttgtgcatgg ctgctttccg
    14161 atttcgacta cgaaggttct accgtgatgc tggctccagg ctccggtttt tataccatcg
    14221 aagggatggg gaaaaatgaa gttcgtatcg cttacgtgct ggaaaaagaa gatttgaaaa
    14281 aagctcttgt cgttctcgaa aaagctttac aagcctatcc cggaaaaaca atctgatgtt
    14341 ggcagatgaa aaagatttga acatttctct gaaaattcaa aatatttttg ttcatcttaa
    14401 tcgataattc ttattgattt gaatgttgaa tatttcatag caaagcgaat tcattttcag
    14461 caggggaaga aaaatgtttc ccgccctgcg gtgcgtattg caaccatcgg tattgcgttg
    14521 ggattggccg taatgttgat ttcggtttgt gtggtgattg gtttcaaaag ccaaatcgag
    14581 aacaaaatca tcggttttgg cggacacatt caaatcacca atttcgacaa caacaatacc
    14641 tacgaaatga atcccataaa aatggatgct gctctgatac aaaagattcg gcaaatttcg
    14701 ggtgtagttc acgtgcagcg ttttgccacc aaaccgggta ttatcaaaac cgataatgaa
    14761 tttcagggta ttattctaaa aggtattgac aaggattttg actggaattt tttccgttcc
    14821 aatttgctgg aaggaaatat tttaaatgtt ggcagcgata cgctgaaaaa cgaaattttg
    14881 ctttctaaaa aattgtccga tttactggga ttgaaattgg gcgacgagct ttttatgtat
    14941 tttattcagg atcaggttag agctcgaaaa tttaaaattg tcggcatata ttccaccaat
    15001 tttgttgatt acgaccagct ctttgtgctt accgatatac gacaggttca gcgtttgaac
    15061 aattgggaca gtgcatcgtt cggtggactt gaggtgttga tagacaattt tgacaaaata
    15121 gatgaacggg gagaagctgt ttatgaagcc acagccaata ttttcagcaa agatggcaat
    15181 gcttactaca cccaaaccat taagcagttg aatcctcaga ttttcagttg gctcgattta
    15241 ttggatatga acgtttgggt aattttgcta ttgatgcttt ccgttgcggg ttttaacatg
    15301 atttccggtt tgctgatttt aattcttgaa cgtaccaata tgattggcat tcttaaatcg
    15361 ttgggtgcaa ccaactggtc ggtaagaaag atttttcttt atcattcttt tttcctgatt
    15421 gcaaaaggaa tgttttgggg taacctcatt ggcctttcca tttgtgctct tcagtatttt
    15481 acgggtatta ttccgctcga tcccgaagct tattatgtgg cttccgtacc ggttgctttt
    15541 ccgtggggtt atattgtatt gctgaatatc ggaacttttg tggcatcaat tttaatgatg
    15601 attgcgccat cttatcttat caccaaaatt tctccagcaa aaattattcg ttacgaatag
    15661 gtgcattttc tattaacaac cgaaaaatta ttgcttttca aattaagtgg taataaccac
    15721 atacatttta atggattgcc aaattaattt ctaaattaat acgcctaata gtagtcgaac
    15781 aaaatcattt ttaatggttt ttccttctca aaaaaattct agttttttag gggctttttt
    15841 acagccattt tttctttttg aaataccaca acattactcc ggcaatgagt agcatgacgg
    15901 cccacatggc aaagtagcca tattgccagt ggatttcagg aaaataatca aaatttgtcc
    15961 cgtacactcc aacaatgaaa gtaagtggaa tgaacagtac cgaaaaaatg gtcagtattt
    16021 tgattatatc attcaaacgg gagcttaggg tattgtgata aatgctcaac tggtcgtata
    16081 gaatttcgcg gtagctatcc attaattcgc ttacttcatt aagatttacc tgtaactcct
    16141 tgaaataggg atggttgtca tcccgaataa attccgattc gagtttggac aggttcagaa
    16201 tcatttcttt ggccggcata atatattttc tcaaataatt caattctctt ttataccgat
    16261 ttatcagcga aggaatatcg ttatccaatt ctttggtcat gagttcttcc acatcttcaa
    16321 ttttatctcc caattgtccg ataaggtaca aataattatc aattaccaca tcgagcaatg
    16381 cacaagccaa ataatcgatg ccggcagtac gaattcgatg atgatgtttt cgtaagcgtt
    16441 cgcgaatggg atcaaaggca tcgccctgat tttcctgaaa tagtatcaag gtgtttttgg
    16501 tgagtataaa actcaaattt tccacgtcca atttgtcaag cttttcatta taagtaagca
    16561 ttttgattac gataaaaagc cctttttcgt agtcctgaac ttgagggcgc gtcatgggat
    16621 ccatgatgtt cgacatgata ttgaagggaa tatcgaaaga ttcggcaatt ttttccatca
    16681 gttttacttc atggagtcct tcaacgttta accagtgaaa tttatctttg ttaagattga
    16741 aattcaattc gcgtaaagat ttaatttctg tttccaccac gctgttttca tcataggtca
    16801 tcaaatacag acgtggtttt tccatttttt tttcgccacg aaaaatcaat tcaaaaggag
    16861 agacgccaat atcttttttc ctttttttga tatattttgc catatgactt atttttttag
    16921 ttcaataaca aagatatacg ataattttga attctgatgc agctttttca attttgatga
    16981 taaagagaag acgaacaaaa aaaaattgtt aaatgagtaa caggttttgc agagaaatcc
    17041 gtctttttct ctgttatctt tatttgaaaa tagtgaagac attaaatatt gaaatgaaac
    17101 gttaacttct cgtgtttgtc ggtttttcat tattgcattt tttcttattt ttgcataaaa
    17161 atcttatcat gaatttagat gtttcggtga ttggctattt ggcggggttt tgtactgctg
    17221 tggcacagtt tccacaagca tacaaagttt acagaaccaa agatacacga agtatttcgc
    17281 tgggtatgta cgcaattatg acttttggcg ttttcctgtg gctaatttac ggaattttga
    17341 ttcaggactg gccaatgatg ttggcaaacg gaattgggtt aattccctgt acattcaatt
    17401 taatcataac ctttcagaac gtttttcgtg aacgaaagaa aatgatataa attggagtaa
    17461 ttaaaaaaat tatcagttta ggttttcgaa actgagaaaa aatttaacgt ttcattatca
    17521 atacgctatg aaacatattc aaattatcaa tgggcctaat ctcaatcttt tgggagaaag
    17581 agagccttcc atttatggaa acaaaagttt taacggtttt tttgaagagt tgaaaaagca
    17641 atatcccgat gtgaaattgg aatatttcca atccaacaat gaaggtgaaa ttatcgataa
    17701 gttgcaagaa attggatttt cggctgacgg aataatttta aatgcaggag cttatacgca
    17761 tacttctatt gcaattgccg atgccattcg ctccattcaa tcgcctgtga tcgaagtgca
    17821 catttccaat gtttttcagc gcgaaagttt tcgtcatcat tcttttttgt ccgaagtatg
    17881 taaaggctgc attatcggat tcggactgaa ctcttatcga ctggctctag aagctttgct
    17941 gcttatttaa aacggattag gaagctggtt gaatttcaaa tttattcatt aattttttat
    18001 gccaaaattt acaaaaataa tagctaccat tagcgataaa agatgcgatc ccgattttat
    18061 tcgcgatttg tttgaagcag ggatgaatgt ggttcggatg aattcggcac atctcaacag
    18121 ggaaggattt ctgaaaatta tcaatagtgt tcgttcggtg agcaacgaaa ttgccctgtt
    18181 gatcgatacc aaaggtcctg aagtgcgaac gaccattgcc gaaaacgata aaataatttt
    18241 acataccggc gacaccatta aaattattgg cgatcccgac aaaatctcga ccaaagaatg
    18301 tgttgcggtc agttatccgt tttttgtgcg cgattt
//
LOCUS       DAOSCH010000031        18110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2147_length_18110_cov_5.998726, whole genome shotgun sequence.
ACCESSION   DAOSCH010000031 DAOSCH010000000
VERSION     DAOSCH010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18110
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2147_length_18110_cov_5.998726"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="PLZ54_03950"
     CDS             complement(<1..1008)
                     /locus_tag="PLZ54_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOJ66118.1"
                     /translation="MKKTIFISAILTLFLMGIMSSCEETLEAPTKSSLDEQVIFSTPV
                     LAEGALMGIHQSLAETNSYRGRYIPFYGINTDIEWYNSSENVSDDRAKLTGYDPLPTN
                     TQMNMENNAWAKMYEAIERANLCIRGLRLYGNVEKNPELAQILGEALTLRAMIYNDLI
                     KAWGDVPARFEPISPSTLYLPKSDRDVIYKQIIADLAEAENYVAWPNETAVTRTTERV
                     NKAFVKGLRARICLAAAGYSQRPDGTIRRSNDPELSVENLYPIVKQECIDVIESKTCK
                     LGTFEQNFRNLCQDNVTAGLESLYEIPFSDGRGRVLYTFGVKHTTTDKYTQQAQGGQN
                     GP"
     gene            complement(1030..4275)
                     /locus_tag="PLZ54_03955"
     CDS             complement(1030..4275)
                     /locus_tag="PLZ54_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ66119.1"
                     /translation="MRTQKKFISILFFILVGFFVQVYGFTDQNNLIQQNERTIKGTVK
                     TTDGEPLIGVSVIVAGSPSAGTVTNLNGDFTITVPANATKLQFSYVGMKPLTVEIGNR
                     SVINVVMTEEAIGLNEVVAIGYGTVKRKDITGSVVSVSSDAIMNIPVSSPAEAISGKL
                     AGVQVTTTEGSPDAEIRIRVRGGGSITGSNKPLLIVDGFPVQSIDDISPYDIESIDIL
                     KDASSTAIYGSRGANGVVIITTKSGKEGKITANYNFYTAAKKIAKTLDVLSPSDYAKW
                     QYELALLKNDGDASSYEKYFGNYQDIDLYNNVPSNNWQDLVFGRTGTTFNHNLSINGG
                     DSKNKFAFSFSHIDDKAIMQMSSFKRDNINFKYTTNPSKNVSMNFSTRYINTKINGGG
                     MNEQKEVSSADSRLKYAMIYPPFPLKDLTSEAGTSDDTEIGNLYNPLVAISDNDRQQK
                     RNDLNMAGDITWKAFDNFKLKVEFGYENYRAEDQRFYGTTTYYIKNIPSSENQGYPAA
                     ELNNTFLNTFRNTNTVSYDFKKLLGTSHHLDVLLGEEYIFNSKQTLTNTIHGYPKSFT
                     STNAFRLTSQGTPYNIENYYSPDDILLSFFGRANYDYLSRYLMSVTFRADGSSKFSKG
                     NKWGYFPSAALAWRISSEPFMESTQSWLDDLKLRLSYGTAGNNNIPSGQMTQSFDSKS
                     TSWINNVASYWSPSKIMANPDLKWETTVTRNLGLDFVLLKSKLNGTIEGYYNTTSDLL
                     INFPVPGTGYDTQYRNMGETQNKGVEFTLNWIAIDKKNYGLSFSGNIGFNKNKINSLG
                     IMSDFGASSGWASTEIGNDYWIAVGGSVGQMYGYRSDGRYEVSDFEGYDSVKKKWILK
                     EGVVDCSSVIGSDALRPGAMKLKDITNDSIVNSKDYTIIGNANPLHTGGFTINGRLYG
                     FDLSANFNWSYGNDVYNANKIEYTSTSKYQYRNMLAIMADGKRWTNLKSDGTISNNPA
                     ELEAMNANTTMWSPYMSKYVFSDWAVEDGSFLRLNTLTLGYTLPVHLIRQIKIQQLRF
                     YVTGYNLYCWTNYSGFDPEVSTRRKTELTPSVDYSAYPKSRQVVFGLNLNF"
     gene            5085..6335
                     /locus_tag="PLZ54_03960"
     CDS             5085..6335
                     /locus_tag="PLZ54_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015506 - nucleoside:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HOJ66120.1"
                     /translation="MSIKFRLILMNFLQYFIWGSWLISLGSYAGNILNFSGVQIGSIF
                     ASMGIVSLFMPGLLGIVADRWIPAHKMLGICHLLGSIFLIAASFQTQYMAFYSFMFIS
                     VMLYMPTISLSNTVAYNALEKNHYDIVKDFPPIRVWGTIGFICAMWTVDFTGFKFSQA
                     QLYVAATAALILGVYSFTLPPCTVNRSNEKKSVIDVLGLRAFTLFKHKKMAIFFIFSM
                     LLGASLQITNGFGDLFLRSFSEIPEYMNSFGVRHSVVLLSISQISETLFILTIPFFLK
                     KFGIKNVMLMSMFAWVFRFALFGMGNPGDRLWMLILSMIVYGMAFDFFNISGSLFVEQ
                     STDSSIRASAQGLFMIMTNGLGATFGSYLAGAIVDKFVTHAEVPNWSTAWYIFAGYAL
                     IVGILFAILFKYKHNTQEVTEKFH"
     gene            complement(6504..9095)
                     /gene="clpB"
                     /locus_tag="PLZ54_03965"
     CDS             complement(6504..9095)
                     /gene="clpB"
                     /locus_tag="PLZ54_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOJ66121.1"
                     /translation="MNFNNFTIKSQEAIQEAVDIVQSKGQQAVETPHLLKGVLLKGED
                     VIQYLFGKMGVNLSMLNSVIDRMIETYPRVSGGEPYLGRETNAVLQKAIDISSKAGDE
                     FVALESILMALVVENNSVKKALNDAGVNEKSLSEAINELRKGSKVNSASAEDTYNSLN
                     KYAINLNERARSGKLDPVIGRDEEIRRVLQILSRRTKNNPILIGEPGTGKTAIVEGLA
                     HRIVRGDVPENLKSKQIYSLDMGAVIAGAKYKGEFEERLKSVVNEVVNSNGEIILFID
                     EIHTLVGAGKSEGAMDAANILKPALARGDLRAIGATTLAEYQKYFESDKALERRFQIV
                     MVDEPDIPSSISILRGLKERYENHHKVRIKDDAIIAAVELSSRYITERFLPDKAIDLM
                     DEAAAKLRLEIDSVPEELDVIERNIKQLEIEREAIKRENDTKKLEQLSQEIANLKEQA
                     DALRAKWASEKSLIDKIQQAKIDIENLKFEAEKAEREGNYGKVAEIRYGKIKALESTI
                     AQYQEELAKIQGNNPLIKEEVDSEDIAEIVSRWTGIPVTKMLQSEKEKLLHLEEELHK
                     RVVGQDDAIQAVADAIRRSRAGLQDPKRPIGSFIFLGTTGVGKTELAKALAEYLFNDE
                     NMMTRIDMSEYQEKFSVTRLIGSPPGYVGYDEGGQLTEAIRRKPYSVVLFDEIEKAHP
                     DVFNVLLQVLDDGRLTDNKGRTVNFKNTIIIMTSNIGSSLIRENFEKINEYNHDEIIE
                     RTKNEVFELLKQTIRPEFLNRIDELIMFKPLDRSQIEDIVRLQLDGVRRMLEQNGIRL
                     ETTDRAVKFIADNGYDPQFGARPVKRAIQKFILNDLSKKIIAGEIVSNQVVVVDADEN
                     GIVFKNK"
     gene            9388..12111
                     /gene="ppdK"
                     /locus_tag="PLZ54_03970"
     CDS             9388..12111
                     /gene="ppdK"
                     /locus_tag="PLZ54_03970"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOJ66122.1"
                     /translation="MDNKKRVYTFGNGQAEGSASMRNLLGGKGANLAEMNLLGIPVPP
                     GFTITTEVCTEYYALGKDKVVELLKPEVEKAMEGVEKLMRSKFGDKKNPLLVSVRSGA
                     RASMPGMMDTILNLGLNDEIVEGLAEKTGNPRFAWDSYRRFIQMYGDVVLGLKPANKN
                     DIDPFEAVIEQLKEERGVKLDTELTVDDLKELVKRFKALVAERTGKEFPLSVYDQLWG
                     AICAVFDSWMNERAIFYRKMEGIPSEWGTAVTVQAMVFGNMGNTSATGVCFTRDAATG
                     EDVFNGEYLINAQGEDVVAGIRTPLQITKQGSQQWAKLTGISEEERKEKYPSMEEALP
                     EIYKELNEVQEKLEKHYKDMQDMEFTVQEGKLWLLQTRNAKRTGTAMVKIAIDMLHQG
                     IIDEQTALLRVDPLRLDELLHPIFDKNAVKKAKVIAKGLAASPGAASGKIVFNADDAA
                     EWAARGEKVIMVRIETSPEDLAGMAAAQGILTARGGMTSHAAVVARGMGKCCVSGAGS
                     LKIDYAQKTMEADGIVLREGDYISLNGSTGDVYLGQIPTQEAELDADFAELMTLADKY
                     SSMKVRTNADTPHDAAVARKFGAVGIGLCRTEHMFFEGEKIKAMREMILAENEEGRRK
                     ALGKILPYQQADFEGIFEVMDGFPVTVRLLDPPLHEFVPHDEKGQKEMAEAMGLPLQK
                     IQQRVEELREQNPMLGHRGCRLGNTYPEITEMQTRAILGAALKLKSKGIKIIPEIMVP
                     LVGIKKEFLEQERIIRETAEKLFAEKGDRIEYKVGTMIEVPRATLIADQIAENAEFFS
                     FGTNDLTQMTFGFSRDDIASFVPDYIEKKIIEKDPFQVLDQEGVGQLIKLGVEKGRST
                     RSDLKIGICGEHGGEPSSIEFCYRVGMNYVSCSPFRVPIARLAAAQAALKN"
     gene            12661..13017
                     /gene="rsfS"
                     /locus_tag="PLZ54_03975"
     CDS             12661..13017
                     /gene="rsfS"
                     /locus_tag="PLZ54_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOJ66123.1"
                     /translation="MEQSEQIVNKIVEGIQEKKGKKVVIINLTGLKNAPCSYFVICEG
                     TTSIQVDAIGTSIKDTVREKMNIKPYAIDGLRNSEWVAMDYGSVIVHIFRPQIRNFYD
                     IEHLWEDAEITEIYDE"
     gene            13075..15078
                     /gene="ftsH"
                     /locus_tag="PLZ54_03980"
     CDS             13075..15078
                     /gene="ftsH"
                     /locus_tag="PLZ54_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOJ66124.1"
                     /translation="METKSPQNSPQKPGKGPKFNITWLYVIIIGILIGSYLFSDTAPV
                     KEVPFSDFEKYMYNNKMEKIDVYSSKNIAEALIIKDSLKQVFGEKYEMYEKEPKIRTR
                     IPSAEEFSKIYQKAKEQYGLTAVVDYKESRNYVDIFLYSILPFLLLILFFVYMNRRMA
                     NQMGGGSGGGIFNVGKSKAQLFDKGDVANRITFKDVAGLEEAKQEVEEIVEFLKNPSK
                     YTELGGKIPKGVLLVGPPGTGKTLLAKAVAGEADVPFFSISGSEFVEMFVGVGASRVR
                     DLFRQAKEKAPCIIFIDEIDAIGRARGKNPNFGSNDERENTLNQLLTEMDGFASNSGV
                     ILLAATNRADILDKALLRPGRFDRQIHVDLPDVHGRKEIFKVHLRPIKIDKSVDIDFL
                     AKQTPGFSGADIANVCNEAALIAARKNKSFVEKQDFLDAVDRIIGGLEKRTKITTKFE
                     KKSIAYHEAGHATISWMLEHASPLVKVTIVPRGEALGAAWYLPEERQLTVRAHMLDEM
                     CSTLGGRAAEELFLNQTSTGAINDLERVTKRAYAMVTYFGMSEKLPNLSYYDSSGSAE
                     YSFTKPYSEKTAELIDEEVKNIVSREYERAKEILREHAEGHNRLAELLIEREVIFSED
                     LERIFGKRPWASRSDELLAQNGENGKSSERMEDNTVENENKEE"
     gene            15059..15724
                     /locus_tag="PLZ54_03985"
     CDS             15059..15724
                     /locus_tag="PLZ54_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HOJ66125.1"
                     /translation="MKIKKNDLINEEELSSREIILKKISDILCTRTSETIFSEYFSHQ
                     IYREIAPDMLTCFMEEIKAVSGECFVYDSPSNLFVALKKFLLGKGFSKIHCRNEKITE
                     YLKSFDIPFSSSEADFEIMEVGITDCEFLVARTGSVIVSSFNSGRKMNIFPPVHIVIA
                     HRSQLVSYLDDAYRQILQKYKNNLPSLISTITGPSRTSDIEKTLVLGAHGPKQLLVFL
                     LDN"
     gene            15790..16602
                     /locus_tag="PLZ54_03990"
     CDS             15790..16602
                     /locus_tag="PLZ54_03990"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOJ66126.1"
                     /translation="MNEMLKRAISGFIYVGLIVTSILVHPLAFAFVFGILTYCALHEF
                     YKITYRPSDIEVPISLASMGGLLLFVSTFLFKSGFSSPLIFSYYAIFVIWMFIRELFL
                     KKSNPIHNWAYFILGQVMVALPMSLLIFILFVDGYQPMLLLSIFILTWLNDTGAYLIG
                     VKFGRHRFFEHISPKKTWEGFFGGIFFSLTGGFILSLFIAELSIFQWLALCFITVLAA
                     TLGDLTESLLKRTLNIKDSGNLLPGHGGLLDRLDSMLLVSPFVLIFFLYIFV"
     gene            16691..17848
                     /locus_tag="PLZ54_03995"
     CDS             16691..17848
                     /locus_tag="PLZ54_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOJ66127.1"
                     /translation="MEMNFDDIRCYTNEEVHEVLEKLTKEKSFMKVVSTAYPLLPKEI
                     IKERLLQYNNQQEFQKDFIFPFLQYLEENTTKGIELFGLEKINVSKAYLYLSNHRDIV
                     MDSSFFCKKLLENSIDTVEIAIGDNLLIYPWIEDFVKLNKSFIVKRGLNTRQTLESSK
                     LLSAYLLHTICEKNQSIWMAQREGRAKDSDDYTQESVLKMLNLSAKDNNFIQHILQFN
                     ICPLSISYEYDPCDYLKAKEFQLKRDFPDYKKSSTDDLENMVIGIMGYKGKVVFRITG
                     CINDELKKIEQKTSNRHEQISQTAQLIDLKIHANYEIYSVNKIAFDTLTGENRFAHEY
                     SNMERIDFEQYLMKQIDKIQLENKDEKFLRQKLLEMYANPLINHLKTLQHS"
BASE COUNT         5638 a         3324 c         3363 g         5785 t
ORIGIN      
        1 cggtccattc tgtccacctt gtgcttgttg agtgtattta tcggtagtag tatgttttac
       61 tccaaatgta tataacaccc gaccacgtcc atcggaaaat ggtatttcat aaagtgattc
      121 taatcctgca gtaacgttgt cttggcaaag gtttctgaaa ttttgttcaa aagttcccaa
      181 tttacaggtc ttgctttcaa tgacatcaat acattcttgt ttaacgattg gataaaggtt
      241 ttcaaccgaa agttcaggat cattacttct gcgaatcgtt ccatcgggac gttgagaata
      301 tccagcagca gccagacaaa ttctggctct taaacctttt acaaaagcct tgtttactcg
      361 ttcagtggtt cgtgtaacag ccgtttcatt aggccaagcc acataatttt ctgcttcggc
      421 caaatcggca attatttgct tgtaaattac atctctgtcc gattttggca aataaagcgt
      481 agaaggagaa atgggttcaa accgcgcagg gacatcaccc cacgctttta tcaaatcatt
      541 gtaaatcatg gctcttaaag taagggcttc gcccagtatt tgggcaagtt ctggattttt
      601 ctccacattt ccataaagtc gcaaacctct aatacataaa ttagcacgtt caatagcttc
      661 atacatttta gcccatgcat tgttttccat attcatctga gtattggtag gaagcggatc
      721 gtaaccggtc agttttgcac ggtcatcact tacattttca gatgaattgt accactcgat
      781 atcggtgtta atcccgtaaa aaggtatgta acgaccacga taggaattgg tttctgccaa
      841 agattgatga atgcccatca aagctccttc tgccaaaaca ggcgtagaaa aaattacctg
      901 ttcgtccaat gacgatttgg tgggagcttc taaagtttcc tcgcatgaac tcattattcc
      961 cattaagaaa agagtgagta ttgcagaaat aaatattgtc tttttcattt tatcaaatta
     1021 tttacaaggt taaaaattta aatttaagcc aaatactacc tgtcggcttt ttggatatgc
     1081 cgaataatca accgatggtg tcaattcggt tttacgtcga gttgaaactt caggatcgaa
     1141 accggaataa tttgtccagc aatacaaatt ataaccggta acataaaaac gcaactgttg
     1201 aattttgatt tgtctgatta aatgaaccgg taaagtataa cctaaagtaa gtgtatttaa
     1261 cctcaagaag gaaccatctt caacagccca atcggaaaaa acatattttg acatatacgg
     1321 agaccacatt gttgtattgg cattcatggc ttccaattct gccggattgt tgctaatggt
     1381 tccgtctgat ttcaaattgg tccagcgttt tccgtctgcc atgatggcta acatgtttcg
     1441 atattgatat ttactggtag aagtgtattc gattttattg gcattgtaca catcattccc
     1501 ataactccag ttaaaatttg cagaaaggtc aaatccgtac aatcttccat taattgtaaa
     1561 tccaccggta tgtaagggat tggcatttcc tattatcgta taatcttttg aatttacaat
     1621 gctgtcattg gtaatatctt tcagtttcat tgctcccggg cgaagcgcat ctgatccaat
     1681 tacggaagaa caatcaacaa ctccttcctt caatatccat tttttcttta cagaatcgta
     1741 tccttcaaaa tcagatactt catatcttcc atcggaacgg tagccataca tttgcccaac
     1801 agaacctcct acagcaatcc agtaatcgtt acctatttca gttgaagccc aaccactgga
     1861 tgctccaaag tcgctcatta ttcccaaaga attgattttg tttttattga atccaatgtt
     1921 tccactgaaa ctcaaaccat aattcttttt gtctattgct atccaattca gtgtaaattc
     1981 aacgccctta ttttgcgttt ctcccatatt acggtactga gtatcataac ctgtaccagg
     2041 aacggggaaa ttgatcaaca agtcggaagt ggtattataa taaccttcaa ttgttccatt
     2101 taatttactt ttcaagagga caaagtccaa tcccaagttt cgcgttacgg ttgtctccca
     2161 tttcaaatcg ggatttgcca taatttttga gggagaccag taagaagcaa cgttattgat
     2221 ccatgaagta gacttggaat cgaaagattg tgtcatctga ccggatggta tgttattgtt
     2281 tcccgctgtt ccatagctca acctgagctt taaatcatcc aaccaagatt gagtagattc
     2341 cataaaaggt tctgaagaaa ttctccatgc taaggcagca gaaggaaaat atccccattt
     2401 attgcctttg gaaaacttac tcgaaccatc ggctctaaat gttacactca tcagataacg
     2461 acttaaataa tcataatttg ctcttccaaa gaatgataaa agtatatcgt cgggtgaata
     2521 ataattctcg atattatagg gagttccttg agaggttaac ctaaaagcat tggtagaagt
     2581 aaagcttttg ggatatccgt gaatagtatt agttaacgtt tgcttactat taaaaatgta
     2641 ttcttctccc agcaatacat ccaaatgatg actagttccc aataatttct taaagtcata
     2701 acttaccgta ttggtatttc tgaaagtatt caaaaatgta ttgttcaatt cggcagcggg
     2761 ataaccctga ttttcggagg aaggaatatt tttaatgtaa taagtggtag ttccataaaa
     2821 acgctggtct tctgcacgat aattctcgta tccaaactca actttcagct taaaattatc
     2881 aaatgctttc cacgttatat ctcctgccat attaagatcg ttccgctttt gttgacgatc
     2941 gttatcggaa atagccacca atggattata taaattccct atttccgtat catcactggt
     3001 accggcttct gatgtcagat cttttaaagg aaaaggagga taaatcatgg catatttcaa
     3061 acgcgaatcg gcacttgaaa cttccttttg ttcattcata cctccaccat tgatttttgt
     3121 gttgatgtat cgagtagaaa aattcattga aacatttttg gaaggattgg tagtgtactt
     3181 aaaattaata ttgtcgcgct tgaaacttga catttgcata atggccttgt catctatatg
     3241 actgaaacta aaagcaaatt tgtttttgga atcgcctccg ttgatgctta aattgtgatt
     3301 aaaagttgtt cctgtcctac caaatacaag atcttgccaa ttgttggaag gtacattgtt
     3361 ataaagatca atatcctgat aattgccaaa atacttttcg taagagcttg catctccatc
     3421 gtttttcaac aaagccagct catactgcca cttagcataa tctgatggag acaaaacgtc
     3481 caatgttttg gctatttttt ttgctgcggt atagaaatta taatttgctg ttattttgcc
     3541 ttcttttccc gatttggtag tgatgataac taccccatta gcaccacgag aaccataaat
     3601 tgccgtagat gaggcatctt tcaaaatgtc aatagattca atatcataag gagaaatgtc
     3661 atcaatagat tgtacaggaa atccatcaac tatcaacaaa ggtttgttgc ttccggtaat
     3721 ggaacctccg ccacgaacac gtattctgat ttccgcatcg ggagatcctt cagtagttgt
     3781 aacctgcacg ccggccaatt ttcctgaaat tgcttctgct ggagaagata cgggaatgtt
     3841 cataatcgca tccgaactaa ccgaaactac agaaccggta atgtcttttc ttttcaccgt
     3901 gccatatcca atggcaacaa cttcattcaa tcctatcgct tcttcggtca ttaccacatt
     3961 aatgacactt cgattcccta tttcaacagt taacggtttc attccaacat aggaaaactg
     4021 caacttggtg gcattagccg gaacagtgat agtaaaatct ccatttaaat tggtaacagt
     4081 acctgcagaa ggagatcccg caacaataac tgatacccca attaatggct ctccatcagt
     4141 agtttttaca gttcctttaa tcgttctttc attttgctga attaaattgt tctggtcagt
     4201 aaaaccataa acttgaacaa aaaaaccaac caatatgaaa aacaaaatgg aaataaattt
     4261 tttttgagtt ctcataaaat tagatttaat taaaagttaa gtatttaaaa ttgatgtgat
     4321 tcttatcctt ctaaatgttt caatataaaa aataatcttt taattttatc gatgctaaaa
     4381 ttaaaatgtg ttcgcacacg gtttttattt caaaattatt tttaatgtgt tgaaaaagca
     4441 atgaaatata tgattataca tttttttaaa cttattactc gtaataacta tatgtctctg
     4501 attaacaagt aattatattg tttttcaaaa ataatgtgta cgcacacgat tttatcacaa
     4561 caaatgtaat atataattct ctatttaatt cactttgaaa aattaaataa tgttaaatat
     4621 atattgttgt tacattcttg tttttgtaat tctctgattt tgtgattttt aaaataaaat
     4681 tttatttccg aaaaatttat gttttgcacg taagcagcga aaaataaaaa aagaacctct
     4741 gaatgctcat gctgataaaa tattaccatc ttaaattatt gctaaataat gtttgatttg
     4801 catggaaaat ttaataacat aaataaggtt gtattttgca attgacttta tagcccgaat
     4861 tggcaattat atcatttgta aacaaaataa ggaataaaac tttgaaaatt tgcttttcca
     4921 actttttatc tatctttgct cccataaaat ttggggagat taatttcctt aatattgtga
     4981 aaaggcatct tttatttgta gatactatca cttttttaaa agctaccaat cttgtggtga
     5041 atttttaaag aaaaatcaaa tataatcact taaattaata cgctatgagt atcaaatttc
     5101 gcttgattct tatgaatttc cttcaatatt ttatttgggg ttcttggttg atttctttgg
     5161 gtagttatgc aggaaatatt cttaacttta gcggagtcca gattgggagt atctttgcct
     5221 caatgggtat tgtttcactt ttcatgcctg gattgcttgg cattgttgcc gacagatgga
     5281 ttccggcaca taaaatgctg ggtatttgtc atttattagg ttcaatattt cttattgcag
     5341 cttcatttca aacccaatat atggcatttt acagcttcat gttcataagt gtaatgctat
     5401 acatgccaac tatttcacta agcaacacag tggcatacaa tgctttggaa aaaaatcact
     5461 acgatattgt caaagatttt cctccaatta gggtatgggg aaccattggg tttatttgtg
     5521 ccatgtggac agttgacttc acggggttca aattttcaca agcgcaactt tatgttgctg
     5581 ctacagcggc tttaattttg ggagtctatt cttttacatt accaccctgc actgttaacc
     5641 gaagcaacga aaaaaaatcg gtaatagatg tattggggtt aagggcattt actttattta
     5701 agcacaaaaa aatggccatc ttttttatat tttccatgct attgggggct tcacttcaaa
     5761 ttaccaatgg ttttggagac ttatttttga gaagtttctc cgaaattcct gaatatatga
     5821 attctttcgg agtaagacat tctgttgtgt tactatccat ttcacaaata tccgaaaccc
     5881 tttttattct tactatacca ttctttttga aaaaatttgg aatcaaaaat gttatgctaa
     5941 tgagtatgtt tgcttgggta tttagatttg cactttttgg gatgggcaat ccaggagaca
     6001 gactttggat gcttattctt tcaatgattg tttatggcat ggcttttgac tttttcaata
     6061 tttccggatc tctttttgtc gaacaatcga ccgattcttc cattcgggca tctgctcaag
     6121 gattatttat gatcatgacc aacggactgg gcgcaacttt tggatcctat ctagccggcg
     6181 ctattgttga taaatttgtt acccatgccg aagttccaaa ttggtcaact gcctggtata
     6241 tttttgccgg ttatgcctta atagtgggaa ttttatttgc tattctcttc aaatacaaac
     6301 acaacacaca agaagttacc gaaaaatttc attgataaaa cacttttttc attgaaatga
     6361 aacaacgtaa atccaagtaa atcaattgtt ttttcaaata atgaaagtat tcggttaaat
     6421 tcaccaaatt ccgttttgta acataaaaag gggccgtctc aaaaaattag gatagccccc
     6481 tctttatttt tcaagttttt tatttatttg tttttgaaaa caataccatt ttcgtcagca
     6541 tcaacaacca ctacttggtt gctaacaatc tctccggcaa ttattttctt cgacaaatcg
     6601 ttcaaaataa atttttgtat tgctctcttt accggccggg ctccaaactg tggatcgtag
     6661 ccattgtcgg caataaactt cactgcacga tcggtagttt ccaaccggat accgttttgt
     6721 tcgagcattc ggcgtacacc atccaattgc agacgaacaa tatcttctat ctgcgaacga
     6781 tcaagtggtt tgaacatgat cagctcatcg atccggttca aaaattccgg acgaatggtt
     6841 tgtttcagca attcaaacac ctcatttttc gttctttcga tgatttcatc atggttgtat
     6901 tcgttaattt tttcaaaatt ttcacgaatc aatgatgaac caatgttgga cgtcatgatg
     6961 ataattgtat ttttgaaatt tacagtacgt cccttgttat cggtcaaacg tccatcgtcc
     7021 agtacctgaa gcaaaacatt gaagacgtca ggatgagctt tttctatttc gtcgaaaagc
     7081 acaacggaat aaggttttct tcgtatggct tcggtgagtt gtccaccttc gtcgtaacct
     7141 acatatcccg gaggcgaacc aataagtcgt gttaccgaaa atttttcctg atactcgctc
     7201 atatcgattc gtgtcatcat gttttcgtcg ttgaacagat attcagccaa tgctttggcc
     7261 aattccgttt taccgacacc agtggtacca agaaagataa acgatccgat aggtcttttt
     7321 ggatcttgca atccggctct actgcgacga attgcatcgg caacagcctg aatagcatcg
     7381 tcctgtccca ccactctttt atgtaattct tcttccaagt gcaaaagttt ttccttttca
     7441 ctttgcaaca tcttggttac ggggattccg gtccagcggc ttacaatctc tgcaatatct
     7501 tcgctgtcca cttcttcctt aatcaacgga ttatttccct gaatttttgc cagctcttcc
     7561 tgatattgag ctatggtgga ctctaatgct tttattttgc cataacgaat ctcggccact
     7621 ttgccataat tgccttctct ttcggccttt tcggcttcaa atttcaaatt ttcaatgtct
     7681 atttttgcct gttgaatttt gtcaatcagc gatttttcac ttgcccattt ggctcttaag
     7741 gcatcggcct gctctttcag attggcaatt tcctgactaa gttgttcgag tttttttgta
     7801 tcgttttcac gtttgatagc ttcacgttca atttccagtt gcttaatgtt tctttcaatc
     7861 acatccagtt cttccggcac agaatcaatt tccaaacgca atttggcggc ggcttcgtcc
     7921 atcaaatcaa tagccttgtc gggaagaaaa cgttcggtaa tataacgact cgaaagttca
     7981 acggctgcaa ttatggcgtc atccttaatt ctcaccttat gatgattttc gtagcgttct
     8041 ttcaaccctc gcaaaatgga aatagaactc ggtatatccg gttcgtccac catgacgatt
     8101 tggaatcgac gttccaatgc tttatccgat tcaaaatatt tttgatattc ggccaaagtt
     8161 gttgcaccaa ttgctcgcaa atcgcctctg gcaagtgccg gttttaaaat gttggcagca
     8221 tccatcgctc cttcgctttt ccctgcgcct accagcgtat gaatttcgtc aataaagaga
     8281 ataatttcgc cattggaatt aaccacttca ttcacaaccg attttagacg ttcttcaaat
     8341 tcacctttat atttggcacc tgctatgacg gcacccatat ccaatgagta aatctgtttc
     8401 gattttaaat tttccggaac atcgcctctc acaattctgt gtgccaaacc ttccacaatg
     8461 gcagttttac ctgttccggg ctctcctatc aaaataggat tatttttggt acgacggctc
     8521 aatatttgca acacacgacg aatttcttca tcccttccta tcacgggatc caacttgccg
     8581 cttctggcac gttcgttcag gttaatggca tatttgttta aagaattgta tgtatcctcc
     8641 gccgaagccg aatttacttt tgagcccttt ctcaattcat taatggcttc gcttaacgac
     8701 ttttcgttaa cacctgcatc attcagtgct ttttttacac tgttgttttc cacaaccaat
     8761 gccatcagga tagattccaa tgccacaaat tcatcaccag cttttgaaga aatatcaatg
     8821 gctttctgca aaacagcatt tgtttctcgt cccagataag gttcgcctcc ggaaacgcgt
     8881 ggataagttt ctatcattcg gtcgattaca gaattcagca tcgacaaatt aacgcccatt
     8941 tttccaaaca aatactgaat aacatcctcg cctttcaaca aaacaccttt cagcaaatga
     9001 ggcgtttcga cagcctgctg tcctttgctc tgaacaatgt ccacagcttc ctgaatagct
     9061 tcctgcgact tgatggtaaa attattgaag ttcataagtt ttatgtttta aaaatttttt
     9121 aactatttca attgtttttc ctttttcgtt caaaacactt accaatcatc aaaagaggaa
     9181 atattgacgt ggtttttcat tcaattaact gataaacaag aaaatatttc ataattactg
     9241 aaaatatgtc atttactcga ttgtggcaaa ttgcaatata aagatgacaa tttcccggtg
     9301 ttttcttttg gtttttaatc aaaataatta tttttgtatt ttgaaattgt tctctctaag
     9361 aattaatttt tttaaatgaa aggaaatatg gataataaga aaagagttta cacatttggt
     9421 aatgggcaag ctgaaggaag cgccagtatg aggaatttat tgggaggaaa aggtgcaaat
     9481 ttagccgaaa tgaatttgct gggtattccc gttccaccgg gttttaccat aaccaccgaa
     9541 gtttgtacgg aatattacgc tttaggaaag gataaagtag tggaactact caaacccgaa
     9601 gtggaaaaag ctatggaagg cgttgaaaaa ctaatgcgtt ccaaatttgg agacaaaaaa
     9661 aatccattgt tggtttcggt gcgctccgga gcaagagctt ccatgcccgg aatgatggac
     9721 accattttga atcttggttt aaatgatgaa attgtagaag gcttggctga aaaaaccggc
     9781 aatccgcgtt ttgcatggga ttcataccgc cgctttattc aaatgtatgg tgatgttgtg
     9841 ttgggattaa aacctgccaa caaaaacgat atcgatccat ttgaagcagt tatcgaacaa
     9901 ttgaaggaag aacgaggcgt aaaactggac accgagctaa cggttgatga cttgaaagaa
     9961 ttggtcaaaa gatttaaggc tttggttgcc gaacgaacgg gcaaagaatt tccgctgtcg
    10021 gtttacgacc aactgtgggg agctatttgt gccgttttcg attcatggat gaatgaacgc
    10081 gccatatttt accgaaaaat ggaaggaata cccagcgaat ggggaactgc tgttaccgtt
    10141 caggctatgg tatttggaaa tatgggaaat acttctgcta caggtgtttg tttcactcgt
    10201 gatgcggcta ccggcgaaga tgttttcaac ggagaatatt taatcaatgc gcagggagaa
    10261 gatgtagtgg ccggtattcg tactccgctg caaatcacca aacaaggttc tcaacaatgg
    10321 gcaaaactta caggcatttc agaagaagaa cgaaaagaaa aatatccttc gatggaagaa
    10381 gcgctgccgg aaatctataa agaactgaat gaagtgcagg aaaaacttga aaaacattac
    10441 aaagatatgc aggatatgga atttaccgtt caggaaggca aactctggct actgcaaacc
    10501 cgtaatgcca aacgtaccgg aacagcaatg gtgaaaattg ccatcgatat gcttcatcaa
    10561 ggaattattg atgaacaaac tgccttgttg agagtcgacc ctttgcgttt ggacgaacta
    10621 ctccacccaa tttttgacaa aaatgccgta aaaaaagcaa aggtcattgc aaaaggactg
    10681 gctgcttcac ccggagccgc cagcggaaaa atagttttca atgctgatga cgctgcagaa
    10741 tgggctgcac gcggagaaaa agttattatg gttcgtatag aaacttcgcc agaagattta
    10801 gccggaatgg ccgcagctca aggaattctt actgctcgtg gcggaatgac ttcacacgcc
    10861 gcagttgtag ctcgcggaat gggaaaatgt tgcgtttcgg gtgccggttc tcttaaaatt
    10921 gattatgctc aaaaaacaat ggaagccgat ggcattgtac tccgagaagg agattacata
    10981 tccctaaatg gttcaacagg tgatgtttat cttggccaaa ttcctacgca ggaagccgaa
    11041 ttggatgccg attttgccga actgatgacg cttgccgata aatattcgag catgaaagtt
    11101 cgcaccaatg ccgatacgcc acacgatgca gccgtagctc gaaaatttgg tgcagttggc
    11161 ataggtttgt gccgtacgga acacatgttt tttgaaggag aaaaaatcaa ggccatgcgt
    11221 gaaatgattt tggccgaaaa tgaagaagga cgccgcaaag cactgggaaa aattctgcct
    11281 tatcagcaag ctgattttga aggaattttt gaagtgatgg atggttttcc ggttacggtg
    11341 cgtctgctcg atcctccatt gcacgaattt gtcccccatg atgaaaaagg acagaaggaa
    11401 atggctgaag ctatgggact tcccttacaa aaaatacaac aacgcgtcga agaacttcgc
    11461 gaacaaaatc ccatgttggg acatcgtgga tgtcggttgg gaaacactta tccagaaatt
    11521 accgaaatgc aaacacgcgc tattttggga gctgccttga aacttaaatc caaaggaatc
    11581 aaaatcatac ctgaaattat ggtacctttg gttgggataa aaaaagaatt tctggaacag
    11641 gaacgcataa tccgagagac tgccgaaaaa ctttttgccg agaaaggtga tcgtatcgaa
    11701 tacaaagtgg gcaccatgat tgaagtaccg cgtgcaacac tgattgccga tcagatagct
    11761 gaaaatgctg aatttttctc gtttggtacc aatgacctca cgcagatgac tttcggcttc
    11821 tcacgagacg atattgcctc ttttgttcct gattacatcg agaaaaaaat cattgaaaaa
    11881 gatccgttcc aagttctcga tcaggaaggt gtcggacagc ttattaaatt aggagttgaa
    11941 aaaggacgtt ctacacgcag tgatttgaag attggaatct gtggtgaaca cggaggagag
    12001 ccatcttcca ttgaattttg ctatcgcgtg ggaatgaatt atgtcagttg ttctcctttc
    12061 cgcgtgccaa ttgcacgctt ggctgcagct caagcagctt tgaaaaattg aggtggcaaa
    12121 atacgattaa agttaattcg aaaacacgcc tactcttatt ctttgcaccg tgcatgcttt
    12181 atgcacggtt tttttgcgtt ttttccatta ttattattat tttctttgaa ttacatggtt
    12241 tgtggtaacc tttttatctt ttgggtgaaa aatttatcct ttttacacgg attggggaac
    12301 aagaaagcaa ttacttttgc aacaatttca agcattattc aataaaatat cacacggcct
    12361 tttaagcaat caagaaaaaa cagaaaaaat caagtcaata aatccgactt tttatctgtt
    12421 gttttcatta ctgatggtaa tagggttgtc ttttgcagac agccttattt ttttgtagca
    12481 gcgccacgac gtatcgggtg caaaagcgta aaaaaattgt tgtttgttaa ggctgtaatc
    12541 aaaattatat ttacaccatt aataaattga tttaaaaaag attatgttta aaaggttaag
    12601 ttttaatttt aataattcat catttcaaat tatctttgta ctaaaaattg gttgtcaata
    12661 atggaacaaa gtgaacaaat agttaataaa attgttgaag gaatacaaga aaaaaaagga
    12721 aagaaagtag tcattattaa tttaaccggg ttgaaaaatg cgccttgcag ttattttgta
    12781 atatgtgaag ggacaacttc gatacaggta gatgccattg ggacttcgat aaaggataca
    12841 gtaagagaaa aaatgaatat aaaaccttat gctatagatg gtttaaggaa tagcgaatgg
    12901 gtggccatgg attacgggtc ggtaattgtt catatttttc ggccacagat tcgtaatttt
    12961 tacgatattg aacatttatg ggaagatgcc gaaataacag aaatttacga tgaatgataa
    13021 aagcaaaaca acaatttaaa aatattcaaa caaaaaaatt aaagagaatt tacaatggaa
    13081 accaaatctc cacaaaatag tcctcaaaaa cccggcaaag gaccaaagtt taatattaca
    13141 tggctttacg tcatcataat aggtattctg ataggttcat atttgttttc ggatactgct
    13201 ccagtaaaag aagtcccatt ttctgatttt gaaaagtaca tgtataacaa caaaatggaa
    13261 aaaattgatg tgtattcttc taaaaatatt gctgaagcac ttatcataaa agattctttg
    13321 aagcaggttt ttggcgaaaa atatgaaatg tacgaaaaag aaccaaaaat caggacaaga
    13381 attccttctg cagaagagtt ttcaaaaata tatcaaaaag ccaaagaaca atatggcctt
    13441 acagctgtgg tcgattataa ggaaagccgc aattatgtag atattttcct ctacagtatt
    13501 ttaccttttc ttttgctgat attgtttttt gtttacatga acaggcgcat ggcaaatcag
    13561 atgggcggag ggtcaggagg aggcattttt aatgtaggta aatccaaggc acaacttttc
    13621 gataaagggg atgtggccaa caggattacc ttcaaagatg tagccggatt ggaagaagcc
    13681 aaacaagaag ttgaagaaat tgtggaattt cttaaaaatc catccaaata tacggaattg
    13741 ggaggtaaaa ttccaaaagg agttttattg gttggtcctc cgggaacagg aaaaacttta
    13801 cttgcaaaag ctgtggccgg agaggctgat gtgccttttt tctctatttc aggatcggaa
    13861 tttgtagaaa tgttcgtggg tgtaggagct tcacgtgttc gagacctttt ccgacaagca
    13921 aaagagaaag ccccctgcat tatttttatt gatgaaatag acgcgatagg acgtgcaaga
    13981 ggtaaaaatc cgaatttcgg gagcaatgat gaaagagaaa atacactcaa tcaactactt
    14041 accgaaatgg atggttttgc ttcgaacagt ggggtcattt tgttggcggc aactaaccgt
    14101 gccgatattc ttgacaaggc gttattgcgt ccgggtcggt tcgacagaca aattcacgta
    14161 gatcttccgg atgtacatgg aagaaaagaa atatttaaag ttcatttacg tccaataaaa
    14221 atcgacaaat cggtagatat agatttttta gctaaacaga cgccggggtt ttcgggtgca
    14281 gatattgcca atgtttgtaa tgaagctgcc ttaattgcag ccagaaaaaa caaatctttt
    14341 gtagaaaaac aggatttcct tgatgccgtt gacagaataa taggaggttt ggaaaaacga
    14401 accaaaatca ctacaaaatt tgaaaaaaaa tccattgcat accatgaagc cggtcatgca
    14461 accatcagct ggatgctcga acatgcaagt cctttggtga aagttaccat tgtgcctcgt
    14521 ggagaagctt tgggggcagc atggtatctt cccgaagaac gtcagcttac cgttagagca
    14581 catatgcttg acgaaatgtg ttctacattg ggtggaagag cagcagaaga actattttta
    14641 aatcaaactt ctacaggagc tattaacgat ttagaaagag taaccaaaag agcttatgcc
    14701 atggttactt acttcggaat gagcgaaaaa ttacccaatt taagttatta cgattcaagt
    14761 ggttcagccg agtattcgtt tacaaaaccc tatagcgaaa aaacagccga gttgattgat
    14821 gaagaggtta aaaacattgt cagtcgagaa tatgaacgag ccaaggaaat tttacgggaa
    14881 cacgccgaag gacacaacag actggccgaa ctattgattg aaagagaagt tattttttcc
    14941 gaagatttgg aaagaatttt cggtaaacga ccttgggcat cacgcagtga tgaattgctg
    15001 gctcaaaacg gggaaaatgg gaaatcatcc gaacggatgg aagacaatac cgttgaaaat
    15061 gaaaataaag aagaatgatt tgattaatga agaagaacta tcttcgcgag aaatcatttt
    15121 aaaaaagatt tcggatattc tttgcacccg tacatcagaa actatttttt cagaatattt
    15181 ctcacatcag atttataggg aaattgctcc cgatatgctt acctgtttta tggaagaaat
    15241 aaaggcagta tcgggagaat gttttgtcta tgacagtcca tcgaacttat ttgttgccct
    15301 gaaaaaattt ttgttgggaa agggattttc caagatacat tgtcgtaatg aaaaaataac
    15361 ggaatatttg aaaagttttg atattccttt ttcatcttcg gaagccgatt ttgaaataat
    15421 ggaagtgggt attacggact gtgaatttct ggttgcccgt accggaagtg taattgtttc
    15481 gtctttcaat tccggaagaa aaatgaatat ttttccacct gtgcatatag ttatagctca
    15541 tcgttcacaa cttgttagtt atctcgatga tgcttacagg caaattcttc agaaatacaa
    15601 aaataatttg ccatcactta tttctaccat aaccggccca agtcgtactt cagatatcga
    15661 aaagactttg gttttaggag cacacgggcc aaaacaattg ctggtcttcc tgttagacaa
    15721 ctaagacgct gtttttcttt ataataaaaa atattgtgta agtttgtaat tttgaaaaca
    15781 taaatccata tgaacgaaat gttaaaacga gcaattagcg gctttattta tgtaggattg
    15841 atagtaacaa gcattcttgt ccatccactt gcttttgctt ttgttttcgg cattcttact
    15901 tattgcgcac ttcatgagtt ttacaaaatc acctatcgac cttcagatat agaagttccc
    15961 atttctcttg cttcaatggg tggcttactt ttgtttgttt ctactttttt gtttaaatct
    16021 ggttttagtt cacccctcat tttttcttat tatgcaattt ttgttatatg gatgtttatc
    16081 agggaattgt tcctcaaaaa aagtaatcct attcataact gggcatattt tatattggga
    16141 caggtgatgg tggcacttcc aatgtcgctg ttgattttta ttttatttgt agatggttat
    16201 caacccatgc ttttgttgtc catatttatt ttgacatggc tcaatgatac cggggcctac
    16261 ctaattggcg ttaagtttgg gaggcatcga ttttttgaac atatatctcc caaaaaaacg
    16321 tgggaaggtt ttttcggagg aatctttttt agtttaacgg gaggatttat attatctctt
    16381 tttatcgctg aactgagcat ctttcaatgg cttgccctat gctttataac agtattggca
    16441 gccaccttag gtgatttaac cgaatcatta ttgaaaagaa ctttaaacat aaaagactcg
    16501 ggaaaccttt tacccggtca tggtgggtta ctcgaccgtt tagacagcat gttgttggtt
    16561 tcaccctttg ttttgatttt ttttctttat atttttgttt gaatttgctg ttctattgtc
    16621 ttttatgaat aataagtaaa tttattgctg agaaaataaa accatattga attgaaaata
    16681 aacagaatct atggaaatga attttgatga catacgttgc tacacaaacg aggaggtaca
    16741 tgaggtgttg gaaaaactaa ccaaagaaaa atcgttcatg aaagtggtta gcacagcata
    16801 ccctttgcta cccaaagaaa taattaaaga acgattactt caatataaca atcaacagga
    16861 atttcagaaa gattttattt ttcctttttt gcagtatctt gaagaaaaca ctaccaaagg
    16921 tattgaattg ttcggtttgg aaaaaatcaa tgtctctaaa gcttatttat acttatccaa
    16981 tcatcgcgat attgttatgg attcaagttt tttttgcaaa aaattattgg aaaattcaat
    17041 agatacagta gaaatagcta taggagataa tttgcttatt tacccgtgga ttgaagattt
    17101 tgtgaagctc aacaaaagtt ttatcgtaaa acgtgggctg aatacacggc aaacgttgga
    17161 aagttccaaa ttactttctg cctatctgct tcatactatt tgtgaaaaaa atcagtcgat
    17221 atggatggct caaagagaag gaagagcaaa ggattcggac gactatactc aggaaagtgt
    17281 attgaaaatg ttgaatttaa gtgcaaaaga taataatttt atccagcata ttttacaatt
    17341 taatatttgt cccttatcca tttcatacga atacgatcct tgtgattacc tgaaagccaa
    17401 agaatttcaa ttaaaaagag acttccccga ctacaaaaaa tcatcaacag acgatttgga
    17461 aaacatggtt atcggcatta tgggatataa agggaaggtg gtgtttcgta ttacaggctg
    17521 catcaacgat gaattaaaaa aaatagaaca aaaaacatcc aacaggcacg aacaaatttc
    17581 ccaaacagca caattgatag atctaaaaat acatgccaat tatgaaattt attcggtgaa
    17641 taaaattgca ttcgatacgc ttactggtga aaatagattc gctcatgaat actccaatat
    17701 ggaaagaatt gattttgaac agtatttgat gaagcaaata gacaaaattc aactggaaaa
    17761 taaagatgaa aaatttttga ggcaaaaatt gttggaaatg tatgccaatc ccttaataaa
    17821 ccatttgaag accttgcaac attcttaacc ttccgtataa aaaagaaaaa ataaaccgga
    17881 acgatttttt caacacaaaa aatcggactt accaaattca ctctccatag atacaatgtt
    17941 taccatttgt aatcatcagt cattatagca aatgggatag tgttttccat aattttttat
    18001 ggaagttgtc ctgaaattgt ttgaaaaaat tatgcaatcg tttgcgttag ttgaaaaaat
    18061 attctacatt taccaccgaa aaaaccaaaa ttaatttttt aaaatgttaa
//
LOCUS       DAOSCH010000032        18106 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2148_length_18106_cov_6.046369, whole genome shotgun sequence.
ACCESSION   DAOSCH010000032 DAOSCH010000000
VERSION     DAOSCH010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18106
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2148_length_18106_cov_6.046369"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLZ54_04000"
     CDS             <1..604
                     /locus_tag="PLZ54_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOJ66128.1"
                     /translation="DYVDIFYSHRYDPNTPIEETMGALSDMVKQGKALYIGLSKYPEE
                     KAREAFRILRENGTPCLIYQDRYNIFSRNIERSHFPLLKENGIGFIAFSPLAQGILTE
                     KYLHGIPEHSRAALSYGFLQKSQITPEVLAKVQELNQMAHQRGQTMAQMALAWCLRTS
                     DVNSVIIGASSVNQIQENIDALENISFTDDELWRIGELGA"
     gene            647..1096
                     /locus_tag="PLZ54_04005"
     CDS             647..1096
                     /locus_tag="PLZ54_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66129.1"
                     /translation="MKIFKTFIFFVLLTLVFIGCDNSSRSSIPDAPVNLQLNMLVAPY
                     NTFNTPNSYLIFDKRMNEIDRIGYGGILLYIDYEGKYCAFDLACPYEVNPNVKVTPND
                     EGKAVCEKCGSVFELLYGIGAPISGPAKEPLKRYRVYLSNNWLYVTN"
     gene            complement(1113..2213)
                     /locus_tag="PLZ54_04010"
     CDS             complement(1113..2213)
                     /locus_tag="PLZ54_04010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ66130.1"
                     /translation="MNFILIGCFILVGSYLLMVIFFIIGWKRLSEFELSPHRMNGKFP
                     VSVVVSCHNEEEHLPRLLSALLRQTFSDFELVLVDDHSTDGTYKIMQEFSSAFSNMKF
                     FPSEIKGKKNALQKAVNIANGELMITTDADCEPCESWIETICNFYFQYRPDLIIAPVK
                     LKSNSKIFPQLQKFEFASLVASGAGAAAFGKSILCNGANLAFPKKIYSIHVNDLHNEL
                     LSGDDMFLLESVKKSKGEIRFLKSTLAMVETKTAENLRDFFRQRKRWSSKFSAYRDKD
                     VITVALLVFLTSFFLLCLFILSLITPRMWKYFLVCFMVKYLIDGYFLYSVRKFFDLRS
                     VWFYAFLLSLIYPFYVLITAVSGVFSGQKSWK"
     gene            complement(2537..3031)
                     /gene="tpx"
                     /locus_tag="PLZ54_04015"
     CDS             complement(2537..3031)
                     /gene="tpx"
                     /locus_tag="PLZ54_04015"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOJ66131.1"
                     /translation="MATVKFAGTDVHTNGELPAVGSKAPDFTLVGKDLQEIHLKDFAG
                     KRVVLNIFPSLDTSTCATSVRKFNQWVSGKNNVVVICVSKDLPFAQSRFCGAEGLDNV
                     ITASDFRYNTFATDYGVLLVDGPLKGLMARAVVAIDENGKVIYNQLVDEISNEPNYDI
                     KVLD"
     gene            3131..4330
                     /locus_tag="PLZ54_04020"
     CDS             3131..4330
                     /locus_tag="PLZ54_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66132.1"
                     /translation="MKLSLLHKLGFFVSIVFLLLSCKDSHAYRVDSAFSTYLYTFQSE
                     AAKRGQVFNFQQEGLIMEFADLTDNKAGLCHYEKPIRIEIDRTYWNDISKYAGAEYMK
                     EDVIFHELGHGFLGREHLNDVLENGDWKSMMCGGQKVNNRAWNINYRGIRRAYYLDEL
                     FNQNTPSPAFSTEDFSVDTSAYTLKVRKSFDTALDAGWKIENTSEYETSIDNRRLKFE
                     SKVDPVYFVYAKTNVDVQSDFIYQLQIECKSKNDSDQYGLIFGSVPDQSASSVQKSLE
                     YFTINNLQKMYMGNTTWYSFYTELTKPCIIPSGNNRMKIVKSGEMLYYFINDVYCYCS
                     EMEVKDSGFYFGFIVPPKSTVWLDNLIVSQQITSQSAVSKVRELPVFDAQVVSEPKFR
                     MKCIKGK"
     gene            4379..6061
                     /locus_tag="PLZ54_04025"
     CDS             4379..6061
                     /locus_tag="PLZ54_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ66133.1"
                     /translation="MSKFVIYQVLPRLFGNYNSNNKYNGSIEENGCGKFNSFTTKALK
                     EIKDLGVTHIWYTGIFEHATQTDYSAYGIRKDFPCLVKGKAGSPYAVKDYYDVDPDLA
                     ENVPDRMKEFEDLIHRTHKEGMKVLIDFVPNHVARQYFSDAKPKNVVDLGEHDHPEWA
                     FSPLNNFYYLPGQPFRPNFDIDGYNEFPAKVTGNDQFTPSPTLNDWYDTVKLNYGINY
                     MEGMQKQFDPIPDTWNKMLDILLFWASKKVDGFRCDMAEMVPVEFWNYAISRVKEKYP
                     KIIFIAEIYKPAEYRNYLANGKFDYLYDKVGMYETLRNVTSRNYPARNITYAWQQLNG
                     IEDHMLNFMENHDEHRIASGFFAGDGSYAQPAMIVAATLTKAPVMIYFGQELGELGMD
                     NEGYSGLDGKTTIFDYWGVKSVQAWANNGKFDGENLTAEQKELRQFYQKLLNITLQEK
                     AITEGVMYDLEYANFNNPHFDTHEQFAYIRKFKDEMLLFVLNFDDKELYTEVRIPLEA
                     FQYLELMPGCQYKAVNLLDEVETIEQITLIPDMIFKISIPAWKGKIFKFKKL"
     gene            6171..6641
                     /locus_tag="PLZ54_04030"
     CDS             6171..6641
                     /locus_tag="PLZ54_04030"
                     /inference="COORDINATES: protein motif:HMM:NF025425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4251 domain-containing protein"
                     /protein_id="HOJ66134.1"
                     /translation="MKAYFGLFAGMALLLISCSGVKTTPESVKSLTQKVENKNFTVKV
                     QYAQPLSGKQIFLTSEYVLTVKNDSAEAFLPYFGVAYSAPYGGDGGIRFNERMTDFSI
                     MPNKRNNGWDIHFKVKAPEDYYEFFLKIFNDGSANFTVNSNRRQSISFMGEVKN"
     gene            7170..8714
                     /gene="zwf"
                     /locus_tag="PLZ54_04035"
     CDS             7170..8714
                     /gene="zwf"
                     /locus_tag="PLZ54_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HOJ66135.1"
                     /translation="MAEQKVLSTKNNIEGQMLVIFGSNGDLARRKLLPSIFQLHADRL
                     LPESFAVIGTGSQEKEQSVFLQEVEESLKKYVKSDSVNDDQTVREFLKKVYYLPVRNQ
                     VDEDFGKLKEYLHSLSAKLNIPRNIIYYFSIPPFLYEVVASNLVKYGLNRQKDGWKRI
                     IVEKPFGYSYETALDLEKKLHAGFQEDQIYRIDHYLGKETVQNIMVTRFANGFFEPIW
                     NSKYVDRVEITAAEKIGVGNRGGYYDTAGALRDMVQNHLLQVMAIVAMEPPAVFDSDS
                     IRNETVKVLQALRPIDEEHVAENVVRGQYIETMVDDVLQKGYRQEKGVASNSRTETYV
                     AMKLFIDNWRWADVPFYIRTGKQLPVQVTEVVIHLKPAPHQLFKQRCITQSTNMIILR
                     LNPDAGIAIDFGMKIPGAGYKVQNVNMAFHYSDLAHSKIPEAYERLILDCMMGDSTLY
                     ARADALRASWKFVDPILNAWKNNPEIPLYFYECNTWGPNEANALFGDKRLKWRPPFNK
                     FKTTEE"
     gene            8750..9502
                     /gene="pgl"
                     /locus_tag="PLZ54_04040"
     CDS             8750..9502
                     /gene="pgl"
                     /locus_tag="PLZ54_04040"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HOJ66136.1"
                     /translation="MKQYDIHVYNTPEDTAKAIAQLISNRINEKQNSLSSFNLAISGG
                     KTPQTLFNILANDFAEKIPWHQLRLFWVDERCVPPTHPESNFGMTNETLLKKVPLPKK
                     NVFRMKGETVPEEEAIHYQSLLTKELPSKNGIPVFDLILLGMGNDGHTASIFPKDINL
                     IDSHLSVAVTQHPDTGQKRITLTGPVLKQADEIIFFVTGASKATILKEIVLNEPPAKK
                     YPAFHIYSVEGKTSFYLDKEAAALLPKELPLL"
     gene            complement(9596..10861)
                     /locus_tag="PLZ54_04045"
     CDS             complement(9596..10861)
                     /locus_tag="PLZ54_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOJ66137.1"
                     /translation="MFGLVDCNNFYVSCERVFNPSLIGRPVVVLSNNDGCVISRSNEA
                     KALGIPMGQPAYQLKELIEKQNVAVFSSNYVLYGDMSHRVMTTLASFVPDVEVYSIDE
                     AFLLLDGFEKQNLKELGRKIVETTVRNTGIPVSMGIAPTKTLAKVANKFAKKFIDYKG
                     LCLIDTDEKREKALQLTAIEDVWGIGRQYSKMLAYQGIRTAWDFTQKSKAWVRRNMSV
                     TGERTWLELQGNPCIEMEHVAAKKSICTSRSFGERLTEYAPVSEAVANFAANCAEKLR
                     KQHSKAMLLTVFIHTNPFSTSQLQYFNQKTVPLPVPTNDSAELIHHALQALKAIFAEG
                     YLYKKAGVVVGDIVPETPLQTDLFDTRDREKFGKAMQVMDSLNATYGKHKVRIAAQGF
                     GRNWKLKNEKLSPFYTTRLKDILEVKATE"
     gene            complement(10914..11387)
                     /gene="umuD"
                     /locus_tag="PLZ54_04050"
     CDS             complement(10914..11387)
                     /gene="umuD"
                     /locus_tag="PLZ54_04050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOJ66138.1"
                     /translation="MKADKIATSGYIDFYSVNTESAFYLPIADGGIAAGFPSPAQDYI
                     DVKIDLNRELIANPSSTFLGRVKGTSMKDAGIEDGDILIIDKSIEPQDGDMAVCFLDG
                     EFTLKYIHMEKDAVYLMPANPKFKPIKVTEENHFCIWGIVTYSIKHHKQKQGKTK"
     gene            complement(12035..12107)
                     /locus_tag="PLZ54_04055"
     tRNA            complement(12035..12107)
                     /locus_tag="PLZ54_04055"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12072..12074),aa:Gly,seq:ccc)
     gene            complement(12224..12799)
                     /gene="rsxA"
                     /locus_tag="PLZ54_04060"
     CDS             complement(12224..12799)
                     /gene="rsxA"
                     /locus_tag="PLZ54_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOJ66139.1"
                     /translation="MTYILIFISAVFVNNIVLSQFLGICPFLGVSKKINTALGMSGAV
                     VFVMTIATIFTYIIQKSILEAYNIAFLQTITFILVIAALVQLVEIILKKISPALYQAL
                     GVFLPLITTNCAILGVAILVVQKNFNLLESVVFAISTSIGFGLALILFSGIREQLSMI
                     KVPKKMEGAPIALLTAGLLALAFMGFSGIVK"
     gene            complement(12863..13453)
                     /locus_tag="PLZ54_04065"
     CDS             complement(12863..13453)
                     /locus_tag="PLZ54_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOJ66140.1"
                     /translation="MSNSKVKIILNGLITENPIFVLILGMCPTLATTSSAINGFSMGL
                     ATSFVLIGSNVAISLLKNLIPDKVRIPAYVVVIATFVTVVEMLMQAYLPALFESLGIF
                     IPLIVVNCIVLGRAEAFASKNSVIDSSLDGIGIGLGFTFALTLLGIVRELLGTGKIFS
                     LSVLPEKYGMLVFVLAPGAFIALAYLIAIMNKIRKA"
     gene            complement(13509..14135)
                     /locus_tag="PLZ54_04070"
     CDS             complement(13509..14135)
                     /locus_tag="PLZ54_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOJ66141.1"
                     /translation="MAKLTSSFKNMVISLLSITVFSALTLGSVYTLTKEPIEKSKTAK
                     LQKALKEVLPAFEHLDTAIKVPVNNVDTLNVYKAYDKNNVFVGAAVEANSKNGFGGEI
                     KIMIGFDKEGNIINYSVLEQKETPGLGTKMVDWFKMSNTKHDIRGKNPAKHRLLVSKD
                     GGEIDAITAATISSRAFLEAVNKAYQAYANNPKAPKTHDVSSGASNIN"
     gene            complement(14128..15096)
                     /locus_tag="PLZ54_04075"
     CDS             complement(14128..15096)
                     /locus_tag="PLZ54_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOJ66142.1"
                     /translation="MNKLIVSPAPHVHSGNSIRKEMVSVLIALIPAYLVALYYFGVGA
                     IIVSVTSVISCVIFEYLIQKFLIKGENTVSDYSAVLTGLLLSFNLPSNLPVWIVVIGA
                     LVAIGVGKMSFGGLGNNPFNPALVGRVFLLISFPVQMTSWPRPMAWNTAYLDAETSAT
                     ALSALKEHLGNIPSLFDMFVGNMGGSMGEVSALALLLGFVYLLFKRTITWHIPVSILL
                     TVFIMTGIFYLVNPQTYVSPIIHLLSGGLMLGAIFMATDFVTSPMTKTGMTIYGIGIG
                     IITVVIRMFGSYPEGVSFAILIMNAFTPLINTYIKPKRFGEVRKNG"
     gene            complement(15115..16452)
                     /gene="rsxC"
                     /locus_tag="PLZ54_04080"
     CDS             complement(15115..16452)
                     /gene="rsxC"
                     /locus_tag="PLZ54_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOJ66143.1"
                     /translation="MKTFKMGGVHPPENKFSAGKKTKIISLPQQVIIPLRHYSGNPSV
                     AEVSRGEIVKVGQLIGRANGFISANVHSSVSGKVSKIDNAMDAFGYKFPAVFIDVIGD
                     DWLDEIDLSPEVKRVCNLEPHEIIKKVAEAGIVGLGGATFPTHVKLSIPQGKKAEVLI
                     INGAECEPYLTCDHQLMLEKGEEIIIGISILMKAIRVKKAIIGIESNKMDAVLNLEKW
                     AKTMEGIEVVPLKVKYPQGSEKQLIQALTGREVPSGALPIEVGAVVQNVATTFAVYEA
                     VQKNKPLIERIITVTGKSVPEPGNYWVRIGTPFTHIIEETGGMPQDTGKVVAGGPMMG
                     RALPSLDLSVAKGTSSILFIPSSESKRGKVFNCIRCAKCVFHCPMGLEPYLLMNQGEK
                     QMWENMEENRVMDCIECGSCSFICPSNRPLVDYIRYGKSTVGTMIRNRKTHKS"
     gene            complement(16512..17375)
                     /locus_tag="PLZ54_04085"
     CDS             complement(16512..17375)
                     /locus_tag="PLZ54_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOJ66144.1"
                     /translation="MNLILASVVSLGGIGALSAVILYFIAQKFHVEEDARIDEVEAVL
                     PAANCGGCGFPGCRAFAAACVTADTLDNLSCPVGGTATMEKVADILGKKAAAVVPTIA
                     VVRCGGTCEMREKTNSYDGVKSCTIVHNLYGGETGCSWGCLGLGDCEVSCTFDAIHIN
                     PETMLPEVDEDKCTACGACVKACPKNIIELRRKGPKSRRIYVNCVNKDKGAVNKKVCK
                     VACIGCSKCFNVCPYEAIEMGNNLAYIDSEKCKLCRKCVEVCPTNAIVELNFPPRKTK
                     IEEPSTTGTEK"
     gene            complement(17415..17834)
                     /locus_tag="PLZ54_04090"
     CDS             complement(17415..17834)
                     /locus_tag="PLZ54_04090"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOJ66145.1"
                     /translation="MQQIEHIGIVQHIEGSKAQILIIQQSACSACHAKSACTVMEEEK
                     KIVDVVVTDSSIKEGDQVVIFGRNSMGLQAVLLAFIFPLLLIVLTLIISNYFQQNETI
                     SGIIALCSIFPYYGILSLFNHKLKRKFEFHVKKESLT"
BASE COUNT         5805 a         3478 c         3314 g         5509 t
ORIGIN      
        1 ggattatgtg gatatttttt attcgcaccg atacgatccg aatacaccca ttgaggaaac
       61 tatgggagcc ctttccgata tggtaaaaca aggaaaagct ctttacattg ggctttcaaa
      121 atatcctgaa gaaaaagccc gggaagcatt ccggatttta cgcgaaaacg gcacaccctg
      181 tttaatctat caagatcggt acaatatttt cagccgaaat attgaacgct cacacttccc
      241 tttattgaag gaaaacggaa tcggatttat tgctttttct ccattggctc agggaatttt
      301 aaccgaaaaa tatttgcacg gaattcctga acattcaaga gctgctttgt cctatggctt
      361 tttacaaaaa tcgcaaatca cacccgaagt gttggccaaa gtgcaagaac tgaaccaaat
      421 ggctcatcaa agaggtcaaa ctatggcaca aatggcgctg gcttggtgtt tgagaacttc
      481 cgacgtcaat tcggttatca ttggtgccag ctcggtaaat caaattcagg aaaacattga
      541 tgctttggaa aatatttcgt tcaccgacga tgaactctgg agaatcggtg aattgggagc
      601 ttagcgcatc aattaccctg actcaataag gaaatttaac tgcaaaatga aaattttcaa
      661 aacattcatc tttttcgtat tgctcacatt ggtcttcatc ggttgcgaca actcatcaag
      721 atcttccatt cctgatgccc cggtgaattt gcagctcaac atgctcgtcg caccttacaa
      781 cacatttaat acgccaaatt catatctgat tttcgacaaa cgtatgaacg aaattgaccg
      841 aattggctac ggaggaattt tactctatat cgactacgaa gggaaatatt gcgccttcga
      901 cctcgcttgc ccttatgaag taaacccaaa tgttaaagtt acgccaaatg atgaaggaaa
      961 agctgtatgc gaaaaatgtg gaagtgtttt tgaacttctt tacggtattg gcgctcccat
     1021 ttccggacct gccaaagaac cactgaaacg ttaccgtgtg tatttgtcaa acaactggct
     1081 ttatgttaca aactgacctt cgagagttta aattatttcc aacttttttg accggaaaaa
     1141 acgccggaaa cagccgttat tagtacataa aaaggataaa tcagcgaaag cagaaaagca
     1201 taaaaccaaa ctgaacgtaa gtcgaaaaat tttcgtaccg aataaagaaa ataaccgtca
     1261 atcagatatt tgaccataaa acaaaccaaa aaatatttcc acattctcgg cgttattaaa
     1321 gacaaaataa aaaggcacag cagaaaaaaa ctcgtcaaaa aaaccaatag agctacagta
     1381 ataacatctt tatctcgata agcagaaaat ttggaagacc atcgcttgcg ctgtcgaaaa
     1441 aaatcgcgca aattttcggc agtttttgtt tctaccatcg ccaaagtcga ttttaaaaat
     1501 ctgatttctc ccttactttt tttcacactc tccaacaaaa acatgtcatc tcccgaaagc
     1561 aattcattat gaagatcatt tacgtgaatc gaataaatct tttttggaaa agcaagattc
     1621 gctccattac acaagatcga tttcccaaaa gctgctgctc ctgctccgga agccaccaaa
     1681 ctggcaaatt caaacttttg gagttgagga aaaatttttg aattactttt tagcttaacc
     1741 ggagcaataa ttaaatcggg acgatattga aaataaaaat tacaaatagt ctcaatccaa
     1801 ctttcacaag gttcacaatc ggcatcggtt gtaatcatca gttccccgtt tgcaatattt
     1861 acggctttct gcaaagcatt tttctttcct ttaatttctg atggaaaaaa cttcatattg
     1921 gaaaatgctg acgaaaattc ctgcattatt ttataggtcc catccgttga atgatcatcg
     1981 accagcacca gttcaaaatc ggaaaaagtc tgacgcaaca aagccgaaag caaccgagga
     2041 agatgctctt cttcattgtg gcaactgacg accacagaaa caggaaattt tccattcatt
     2101 cgatgaggag aaagttcaaa ttcagacaat cttttccaac ctatgatgaa aaaaatcacc
     2161 ataagcaggt aagaaccaac caaaataaaa caaccaatta ggataaaatt cattgttcaa
     2221 aaaatgattt aaaccaatta attaatagac cttttatgag aataacagat aaaaagttct
     2281 ttttcaaaat atttgataaa agaacttttt ttgctttgat gaaacaaatg ctttactttg
     2341 attttccttt tatcaaaagc cacaaaaata taacatttta ctgaaaaaca aagacaaaca
     2401 aattatgtta aaaattaaac aggttgtcaa taagttgaaa atgaaaaagg gtaatttgtt
     2461 tattttcaac gcaaaaacac cctctcccac aaaataagtg aaagagggtg ttttttaaaa
     2521 gaaaatagac cttttatcag tcgagcactt ttatatcgta attcggctcg ttgctgattt
     2581 cgtcaaccaa ctgattgtaa ataactttac cattttcatc gatggctact acggcacgag
     2641 ccattagtcc tttcagcggg ccatcaacca gcagaacccc ataatcggta gcaaaagtat
     2701 tgtatctgaa atcggaagcc gtgataacat tgtcaagtcc ttctgctcca caaaatctgc
     2761 tttgggcaaa gggcaaatcc tttgaaacgc aaattaccac aacattgttt ttcccggaaa
     2821 cccactgatt gaattttcta acggaagttg cacaagtgct ggtatccaag ctgggaaaaa
     2881 tattcagcac gacgcgtttg ccggcaaaat ctttcaagtg aatttcctga agatcttttc
     2941 caaccaatgt gaaatcggga gctttggaac ccactgcagg caattcgcca ttggtatgta
     3001 cgtctgttcc cgcaaattta actgtagcca taaaattaag tattaaaatg tttaaaaatt
     3061 attttcattt taaactaata acaaagctat gacgtttttt gtttaaattt gtgtggaaaa
     3121 atttttaaca atgaaattat ctctgctcca taaacttggt ttttttgtaa gcattgtttt
     3181 tctactcctt tcctgtaaag atagccatgc ctatcgagta gattcagcct tttccactta
     3241 tttgtacacc tttcagtccg aagccgcaaa aagaggtcag gtttttaatt ttcaacaaga
     3301 aggtttgatc atggaatttg ccgatttaac tgacaacaaa gcaggacttt gtcattatga
     3361 aaaaccgata cgaatagaaa ttgaccgaac ttactggaat gatatttcaa aatatgccgg
     3421 ggctgaatat atgaaagaag atgttatttt tcacgagttg ggacatggtt ttttgggccg
     3481 tgaacatttg aatgatgtgt tggaaaatgg cgattggaaa tcaatgatgt gtggaggcca
     3541 gaaggtgaac aatagagcat ggaatataaa ttatcgcggc atcagaagag cctattatct
     3601 cgatgagctt ttcaatcaaa ataccccttc tcctgcattt tctacagaag atttttcggt
     3661 agatacttcc gcctatactc tcaaagttcg taaatctttc gatacggcgc ttgatgccgg
     3721 gtggaaaatc gaaaacactt ccgagtatga aacttccatc gataatcgcc gtctaaaatt
     3781 tgagtcaaaa gttgatcctg tttattttgt ttatgccaaa acgaatgtcg atgtacaatc
     3841 agattttatt tatcagcttc agatagaatg taaaagcaag aacgattccg accagtatgg
     3901 tttgattttt ggttcagtgc ccgaccagag tgcatcttcc gtacaaaaat cgctggagta
     3961 ttttaccatt aataatttgc agaaaatgta tatgggaaat acaacatggt acagttttta
     4021 taccgaatta accaagccat gcattattcc atccggaaat aaccggatga aaatagtaaa
     4081 atccggcgaa atgctttatt attttatcaa tgatgtgtat tgttactgtt cagaaatgga
     4141 agtgaaagac agcggttttt attttggatt cattgttccg ccaaagagta cggtttggtt
     4201 ggacaattta atcgtatctc agcaaatcac ttcacagagt gcagtatcaa aagttcgaga
     4261 attgcctgta tttgatgctc aagttgtttc tgaaccgaaa tttaggatga agtgtattaa
     4321 aggcaaataa aaattagaag aaaaactttt ttgtttaaaa ccaacaaatt cagtcattat
     4381 gtcaaaattt gtaatatacc aggttttacc gcgtcttttc ggtaattata attccaacaa
     4441 caaatacaat ggaagcatag aagaaaatgg atgcggaaaa tttaacagct ttacaacaaa
     4501 agccctgaaa gagataaaag acttgggagt tacccatatt tggtacaccg gaatttttga
     4561 gcatgctacc caaacagatt attctgctta cggcatcaga aaagattttc cttgtctggt
     4621 aaaagggaaa gcagggtctc cttatgccgt aaaagattac tacgatgtcg atcccgattt
     4681 ggctgaaaac gtccccgaca gaatgaaaga atttgaagat ttgattcacc gaacccacaa
     4741 ggaaggcatg aaagttttga ttgattttgt gcctaatcac gtggcgcgac aatatttttc
     4801 ggacgccaag ccaaaaaatg tcgttgactt gggcgaacat gatcacccgg aatgggcttt
     4861 ttctccgctc aacaattttt attatttgcc cggtcaaccc ttccgtccca attttgatat
     4921 tgatggctac aatgaatttc cggcaaaagt taccggaaac gaccaattca ccccttctcc
     4981 aacgctgaac gactggtacg atacggtgaa gctgaattat ggcataaatt atatggaagg
     5041 aatgcaaaaa caattcgatc ctatccccga tacatggaat aaaatgttgg atattttgct
     5101 tttctgggca tcgaagaaag ttgacggctt tcgttgcgac atggccgaaa tggtgccggt
     5161 agaattttgg aattatgcca tcagcagagt aaaggaaaaa tatccgaaaa ttatttttat
     5221 tgcagaaatc tataaaccgg ccgagtatcg gaattatctg gcaaacggga aattcgatta
     5281 tctttacgat aaagtaggta tgtacgaaac gcttcgaaac gtaaccagcc gaaattatcc
     5341 cgcccgcaac atcacttatg cttggcaaca gctgaacggt atcgaagatc acatgcttaa
     5401 ttttatggag aatcatgatg aacatcggat cgcttccgga ttttttgcag gcgatggcag
     5461 ttatgcgcag ccggctatga tagtggcagc aactctcacc aaagctcccg ttatgattta
     5521 tttcggtcag gaacttggtg aattgggaat ggataacgaa ggatacagcg gactcgacgg
     5581 aaaaacaact attttcgatt attggggagt aaagtccgtg caggcatggg caaacaatgg
     5641 aaaatttgat ggagaaaatt taactgcaga acaaaaggaa ctgcgacagt tttatcaaaa
     5701 gttgttgaat attacgctgc aagaaaaggc tataacggaa ggggtaatgt acgatttgga
     5761 atatgccaat ttcaacaatc ctcatttcga tacacacgaa caatttgcct acattcgaaa
     5821 gttcaaagac gaaatgctgc tgtttgttct gaatttcgac gataaggaac tttacaccga
     5881 agtgcgtatt cctttggagg cttttcaata cttggaactt atgccgggtt gtcagtacaa
     5941 agcagtcaat ttacttgatg aggtggaaac gattgaacaa attacactca tacccgatat
     6001 gatttttaaa atttccatcc cggcttggaa aggtaaaatt tttaaattca aaaagctgtg
     6061 aatcaggaag tttttgttat aaattttttt gacaaaataa tggaaatgga atattttttg
     6121 atgaattaaa actgacaatt attcttgttt tacttataaa tctgtacact atgaaagctt
     6181 attttggctt gtttgcaggc atggctttat tgctgatttc ctgctccgga gttaaaacca
     6241 ctcccgaaag tgttaaatct cttacccaaa aagtggaaaa taaaaatttt acggtgaaag
     6301 ttcagtatgc tcaacctctc tcaggaaaac agattttttt gacgtccgaa tatgtactga
     6361 cagttaaaaa tgactccgcc gaagcatttt tgccctattt cggagtggct tattcagctc
     6421 cctatggtgg cgatggcggt attcgtttca atgaacgcat gaccgacttt agcataatgc
     6481 ccaataaaag aaacaatggt tgggatattc attttaaagt caaagcgccg gaagattact
     6541 atgaattttt tctgaaaatc ttcaacgatg ggagtgccaa ttttacggta aattcaaata
     6601 gacggcaatc aatttcgttt atgggagaag tgaaaaatta gctaatgcta attgtagaaa
     6661 cttctttcct tacggtcgaa gaagatatac aaattaaccc tatatggaat gcttctgctt
     6721 gaaatttgaa aaatatcaga aaaggtcaac ttttttattt ttttgattga atgtgtccta
     6781 aaattttata agcgtcccat ttaattaaaa gagtttttaa aaattgattg tttagtcaac
     6841 gtaaatcctc cgttcagtca gaattatttt taaaactgct gaaatagagg atagaaagtg
     6901 aagaattgtt attttaggaa actgtttgat aacagagagc gttgataaaa ttttttcctc
     6961 attcgacatt aaaaatcatt ttaaaaaaca catcatttca ataaactttt gttcaattgg
     7021 gttgtttaca caatgataag atgaaaaaag ttttgcaatg ttattttgta ttcaagacaa
     7081 tgcaataaat tcgatttttt aaaacgtaaa aacacaaata attgtttatc aatcaaatca
     7141 aataaaataa aataaaataa aaatataaaa tggctgaaca aaaggtctta tctacaaaaa
     7201 ataatattga agggcaaatg ctggtaatat tcggttcgaa tggagattta gccaggcgca
     7261 agttgctgcc ttcaatattt caactgcatg cggatcgtct tctacccgaa agttttgccg
     7321 taattggaac cggcagtcag gaaaaggaac aatccgtttt tttgcaggaa gtggaagaaa
     7381 gtctgaaaaa atatgtaaaa agcgattcgg taaacgatga tcagacagtg agggaatttt
     7441 tgaaaaaagt ctattacttg cccgtcagaa accaggtgga tgaagatttc gggaaactga
     7501 aagaatatct tcattccctt tcggcaaaac taaatattcc gcgtaatatt atttactatt
     7561 tttctatccc tccttttcta tacgaagtgg ttgcttccaa tttggtaaaa tatggactga
     7621 acagacaaaa agacggttgg aaaaggatta tcgtggaaaa accgtttggt tacagctatg
     7681 aaacagcatt ggacttggaa aaaaaacttc atgccggctt tcaggaagat caaatttacc
     7741 gtatcgacca ttatctggga aaagaaacgg tgcaaaacat tatggttaca cgttttgcca
     7801 acggtttttt tgaaccgata tggaacagca aatatgtgga cagagtagaa ataacggctg
     7861 ccgagaaaat tggcgttggc aatagaggcg gatattacga tactgcgggt gcacttcgcg
     7921 acatggtgca aaatcatttg ttgcaggtaa tggctattgt tgcaatggaa ccgcctgccg
     7981 tttttgattc cgattccatc cgaaacgaaa cggttaaggt gcttcaagct ttgcgaccca
     8041 ttgatgaaga acatgttgcc gaaaatgtgg tgcgtggcca gtatatcgaa actatggtag
     8101 acgacgtgct gcaaaaaggc tatcgtcagg agaagggagt tgcgtccaat tccagaaccg
     8161 aaacgtatgt tgccatgaaa ctttttatcg acaactggcg ttgggcagac gtaccatttt
     8221 atattcgcac cggtaaacaa ttacctgttc aggttaccga agtagtcatt catttaaaac
     8281 cggctcctca tcagttgttc aagcaacgtt gcattactca gtccaccaat atgattatac
     8341 ttcgtctcaa tccggatgca ggcattgcca tcgatttcgg tatgaaaatt cccggcgctg
     8401 gctacaaagt tcaaaatgtc aacatggcat ttcattattc cgacttggcg cattcgaaaa
     8461 ttccggaagc ttatgaaaga ctcattttgg actgcatgat gggagattcc acactttatg
     8521 cccgagccga tgcactgaga gcaagctgga agtttgtaga tcctattttg aatgcttgga
     8581 aaaataatcc tgaaattcca ctctattttt acgaatgcaa tacgtgggga cccaatgaag
     8641 ccaatgccct ttttggcgac aaacgtttga aatggaggcc tccattcaac aaatttaaaa
     8701 caaccgaaga ataaattatc aattctcatt tctttataca aagttcgcaa tgaagcaata
     8761 tgatattcat gtttacaata cgccggaaga tacagcaaaa gctattgctc agcttatttc
     8821 gaatcggata aacgaaaaac aaaattcatt atcatccttt aatttggcta tttcgggagg
     8881 aaaaactcca caaacacttt ttaatattct ggctaatgat tttgcggaaa aaattccgtg
     8941 gcaccaactt cgcctgtttt gggttgacga acgttgcgtc cctccgacgc atccggaaag
     9001 caactttgga atgactaatg aaacgctgct gaaaaaagtt cctcttccta aaaaaaacgt
     9061 ttttcgtatg aagggagaaa ctgttccaga agaagaagcc attcattatc aatcgcttct
     9121 gacaaaagaa cttccatcaa aaaacggaat tcccgttttc gacctgattt tgttgggaat
     9181 gggaaatgat gggcataccg cttccatttt tccaaaagac ataaatttga ttgacagtca
     9241 tttatctgta gcggttaccc aacatcccga cacaggacaa aaaagaataa cgctaactgg
     9301 gcctgtgctg aaacaggcag acgaaataat attttttgtt accggtgctt caaaagcgac
     9361 aattttaaag gaaatcgttc tgaatgagcc tcctgcaaaa aaatatccgg cttttcatat
     9421 ctattcggta gaaggaaaaa cttcttttta tctggacaaa gaagccgcag ctcttctacc
     9481 caaagaattg ccattgttgt aatatttttt gtttattcat tgatttcaaa aatccgccaa
     9541 ttcaaatttt gattgataaa acatttcaac ttgatagcaa agaaagtcaa aaaatttatt
     9601 ccgtagcttt gacttccaga atatccttta gtcgggtagt gtaaaaagga gaaagttttt
     9661 cgtttttcaa tttccagttg cgcccaaaac cttgtgcagc aatacgtact ttgtgtttcc
     9721 cgtaggtagc atttaagcta tccattacct gcatggcttt accgaatttt tccctgtcgc
     9781 gcgtatcgaa caaatcggtc tgaagcggag tctccggaac aatatcgcct acaacaactc
     9841 ctgctttttt gtacagataa ccttcggcaa aaatagcttt caaagcctgc agtgcatgat
     9901 gaatcaattc ggctgaatcg ttggttggaa caggtagagg aacggttttc tggttaaaat
     9961 attgcaattg tgaggttgag aaagggttgg tatgaataaa aaccgtcagc agcatagctt
    10021 ttgaatgttg ttttctcaac ttttcggcac agttggctgc aaaatttgct accgcttccg
    10081 atacgggagc atattcggtg agtctttcac caaaacttcg agaagtacaa atagattttt
    10141 tggcagctac gtgttccatt tcaatgcacg gattcccttg caattcgagc caagtacgtt
    10201 cgcccgttac actcatattt cggcgtaccc aggctttcga tttttgagta aaatcccaag
    10261 ccgtacgaat accctgatat gccagcattt tggaatattg tctgccaatt ccccaaacat
    10321 cttcaatggc agtgagttgg agtgctttct cacgtttttc gtcggtatca attaaacaaa
    10381 gtcccttgta atctataaat tttttggcaa atttattggc tacttttgcc agtgttttgg
    10441 taggagcaat acccatactg acaggaatgc cggtgttgcg tacggtagtt tccacaattt
    10501 ttcttcccaa ttcctttaaa ttttgctttt caaacccatc taacaacaaa aaggcttcat
    10561 ctatggaata cacttccaca tcgggcacaa aagatgccaa tgtagtcatc accctgtgag
    10621 acatatcacc gtacaaaaca taattggaag aaaaaacggc aacattttgt ttttctatca
    10681 gttccttcaa ttgataggca ggttgtccca tcggtattcc cagtgctttg gcttcgttgg
    10741 aacgggaaat cacacatccg tcgttgttgg acaataccac aacgggacga cctatgagcg
    10801 acggattaaa aacgcgttca caggagacat aaaaattatt gcaatctacc aacccaaaca
    10861 ttttgcacat ttttttcttc ttttccctca atttcgggaa aaatttcttg gacttatttc
    10921 gtttttccct gcttttgttt gtgatgcttt atggaatacg taacaattcc ccaaatgcag
    10981 aaatgatttt cctccgttac cttaataggc ttgaatttcg gattagccgg cattaaatac
    11041 acagcgtcct tttccatgtg gatatatttc agtgtaaatt cgccatccag aaaacatacc
    11101 gccatatcgc catcctgcgg ttcgatcgac ttgtcgataa tcagaatatc tccgtcttca
    11161 ataccggcat ctttcatcga tgttcccttt acccgtccca aaaaagtact ggaaggattg
    11221 gcaataagtt cacgattcaa atcaattttc acatcgatgt aatcctgcgc cggactcgga
    11281 aatcctgctg caattcctcc atctgcaata ggcagataaa aagcagactc cgtatttacc
    11341 gagtaaaaat caatataacc tgatgttgcg attttatcag ctttcataaa tttttgttga
    11401 catgaataaa tcggtttttg tttgttcttt caacacaaag taacagattt ttttgaaaaa
    11461 aaaggaaaga gcaaagtaaa agaaagcata aaaattgaaa aaatcaacaa tttattgtat
    11521 gtcaatgcga taaaaaatta ggagttactc ttttaatagt ccggtttacg cggcaagatt
    11581 ataaatgttt gtgaatagaa atagaaacaa tgcttcgata aataattctc ttataaaaag
    11641 ttagaaattg attgcacaaa tggttatcct acttttcaaa ttaagatcag gaaatgtcat
    11701 aatattgaga aacccgtatg attacataca caaacacgaa tgaatataat tttagacatg
    11761 tgggttccat cagaccatta aaaaataaat catattctga tgaaaagaag tttaaaaatt
    11821 agagtttttc atcccaaaaa atataatgta ctatagtctg aagcgttcgc aaacgggctt
    11881 ggataagttg ttttttacct agatactttt ttacaagcaa gtgctttttg caggtgagcc
    11941 catgtttttg caggtgagcc atttctattt ctatttctat ttcaatatcc aataaaaaga
    12001 gactgtccca acttttgaga cagccccatt tttgagcggc aaacgggact cgaacccgcg
    12061 acctttagct tgggaagcta aagctctacc aactgagcta ctgccgcaaa tagaaataca
    12121 aaagtaaatt aaattttata aacgacaaga agttttcaaa aaatccctta gatatggatt
    12181 ataattcttt tgttgtgtta aatatcatat tccccgaaaa aatttatttt acgataccgg
    12241 agaaacccat aaatgccaaa gccagcaaac cggcagtaag taatgctatg ggtgctcctt
    12301 ccattttttt cggaactttt atcatgctta attgttctct aatgcccgaa aaaagaatta
    12361 atgccaaacc gaaaccaatg gatgtagata tggcaaatac tacactttca agcaaattga
    12421 agtttttctg aaccaccaaa atagctacgc caagaatcgc gcaattggta gtaattaaag
    12481 gaagaaaaac tcctaaagcc tgatatagcg ccggggaaat tttttttaag ataatttcaa
    12541 ccagttgtac caaagctgca ataaccagaa tgaaggtaat agtttgtaaa aaagcgatat
    12601 tgtaagcttc cagaatggat ttttgaatga tatatgtgaa aatagtagca atagtcatta
    12661 caaaaactac tgctccactc attcccaaag ccgtattaat ttttttggat acccccaaga
    12721 atggacaaat tcctaaaaat tgagaaagaa caatgttgtt tacaaagacg gcactgatga
    12781 aaattaaaat ataagtcatg gcattgtaaa tatttaatca gttcaacaat ttaatatgtt
    12841 tcaaaaataa atctatcagg ttttatgctt ttctaatctt gttcataatt gcaatcagat
    12901 aagcgagagc aataaaagct cccggagcca gaacaaatac cagcattccg tatttttcgg
    12961 gtaatacact cagactgaaa atttttcctg ttccaagcaa ttctcttact atgcccaaca
    13021 atgtcaatgc aaaagtaaat cccaatccaa tgccaatgcc atcaagggac gaatcgataa
    13081 ctgagttttt tgatgcgaaa gcttcagccc tgcctaatac gatacaattg acaactatta
    13141 agggaataaa aataccgagg ctttcaaaaa gtgctggtaa atatgcctgc ataagcattt
    13201 ccacaactgt tacaaatgtg gcaataacaa ccacataagc agggatacgc actttgtcgg
    13261 gaataagatt ttttaacaga gaaatggcca cattggaccc aattagtaca aatgaagtgg
    13321 ccaaacccat actaaaaccg ttgattgcag aagatgtggt agccagcgtt ggacacattc
    13381 ccaatatcaa tacaaaaata ggattttcgg tgattaaacc attgagtatg attttaactt
    13441 ttgaattact cattgtttta ttttttgcgg ataataatcg taataaatat ataaaaatat
    13501 gtaattactt aatttatatt tgatgctccc gatgaaacat catgggtttt tggtgctttg
    13561 ggattgttgg cataagcctg atacgctttg ttcacggctt ccaaaaaagc tcttgagcta
    13621 atggttgctg cagtgatggc gtcaatttct cctccatcct tgctgaccaa aagacgatgt
    13681 ttagcaggat tttttccacg aatatcgtgt ttggtgttgg acattttaaa ccaatctacc
    13741 atttttgtcc ccaaaccggg agtttctttt tgttccaata ccgaataatt tatgatattt
    13801 ccttccttgt cgaaaccaat cataattttt atttctcctc cgaatccatt ttttgagttg
    13861 gcttctaccg cagcacctac aaaaacgtta tttttgtcat aggccttgta cacattcagt
    13921 gtatcgacat tatttactgg aactttgatg gcagtatcca aatgttcaaa agccggcaat
    13981 acttctttca gtgctttttg taatttggca gtctttgact tttctattgg ttctttggtg
    14041 agtgtatata ctgagccgag tgttaaagcc gaaaaaactg ttatcgacaa aagtgaaatc
    14101 accatatttt taaaagaaga tgttaattta gccattctta cgtacctccc caaaacgttt
    14161 aggtttaata tatgtattaa ttagtggtgt aaacgcattc atgataagga tagcaaaaga
    14221 tactccttcg ggataggaac caaacattct gataacaaca gtgataatac caatgccaat
    14281 gccgtaaatt gtcattcccg tttttgtcat gggagaagta acaaagtcgg tggccataaa
    14341 gatagctccc agcattaatc cgcccgacaa taaatgtata attggggaaa cataagtctg
    14401 aggattaaca agatagaaaa tgcctgtcat gataaataca gttagtaaaa tactcacagg
    14461 aatatgccag gtaatggtac gtttaaagag taagtaaaca aatcctaaca gcaaagccaa
    14521 tgcagaaact tctcccatag atcctcccat atttcctaca aacatatcga acaaagaggg
    14581 tatgtttccc aaatgttctt tcaaagcact taaggctgta gctgaggttt ctgcgtcaag
    14641 gtaagcagta ttccaagcca tagggcgagg ccaagatgtc atttgtacgg gaaaggaaat
    14701 caataaaaaa acacgaccca ccagggcggg attgaaagga ttgtttccca gtcccccaaa
    14761 actcattttg cctacgccaa ttgctaccaa agctcctata acaacaatcc atacgggcag
    14821 attggaagga agattaaaag atagcaatag tccggttaaa acagctgaat agtcggaaac
    14881 agtattttct cctttgatta aaaatttttg aattaagtat tcaaaaatta cacatgaaat
    14941 caccgaggtt acacttacaa tgatagcgcc aactccaaaa taatacagag ccaccagata
    15001 tgcaggaatg agtgcgatca gtacactgac catttctttt ctgatggaat tgccactgtg
    15061 tacgtgtggg gcaggcgaaa cgattaattt attcatattt ttaatttttc tgtcttatga
    15121 tttatgtgtt ttcctgtttc gaatcatcgt tccgacggta gattttccat aacgaatgta
    15181 atcgaccaaa ggtctgttgg aagggcaaat aaaactgcat gaaccacatt ctatacaatc
    15241 cattacacga ttttcttcca tattttccca catctgtttt tctccctgat tcataagaag
    15301 atacggttct aagcccatcg ggcagtgaaa aacacattta gcacagcgaa tgcaattaaa
    15361 tacttttcct cttttcgatt ccgaggaagg gataaacaat atggaagatg tccctttagc
    15421 caccgacagg tcgagacttg gcaatgcacg tcccatcata gggcctccgg ctacaacttt
    15481 tccagtatcc tgaggcattc ctcccgtttc ttctataata tgtgtaaagg gagttcctat
    15541 acgaacccaa tagttacctg gttcgggtac tgatttgcca gttaccgtaa tgattcgctc
    15601 aataagcggt ttattttttt ggacggcttc atacacggca aaagttgtgg ctacattttg
    15661 aactactgcg ccgacttcaa taggtaaagc tccactggga acttctcgtc ccgttaatgc
    15721 ctgaatcaat tgtttttcac ttccctgtgg gtatttgact ttgagaggaa ctacttctat
    15781 gccttccatt gttttcgccc atttttccaa gttgagcaca gcatccattt tattgctttc
    15841 gattccaata attgcttttt ttactcgaat tgctttcatg agtatgctga tgccgatgat
    15901 aatctcttct cctttttcca gcattagctg atggtcgcag gtgagataag gttcacattc
    15961 tgctccgttg ataataagta cttcagcttt tttcccctga ggaatagata attttacgtg
    16021 cgttgggaaa gttgctcctc ctaatccaac tattccggct tctgcaactt ttttaattat
    16081 ttcgtgaggc tcaaggttac atactctttt tacttcaggc gacaaatcaa tttcatcgag
    16141 ccaatcgtca cctattacat caataaatac tgccggaaat ttatatccga atgcatccat
    16201 tgcattatca atttttgata cttttcccga tactgaggaa tgaacattgg cagaaataaa
    16261 tccatttgct cgtccaatca attgaccaac ttttactatt tcacctctgg atacttctgc
    16321 tacgctcgga ttgcctgaat aatgacgaag tggtataatc acctgctggg gcaacgagat
    16381 tatttttgtt ttttttccag ctgaaaattt attttccggg gggtggacac cgcccatttt
    16441 aaatgtcttc attaaaagtt tatgattttt tcttatctat ttaaatcaaa taaaaattgt
    16501 gttgaaaaaa cttatttctc agttcctgtt gtagaaggtt cctctatttt tgttttacgt
    16561 ggtggaaaat tcaactccac gatagcatta gttgggcaaa cttcaacgca tttgcggcat
    16621 aatttacact tttcggaatc gatataagcc aaattgttcc ccatttcaat ggcttcgtag
    16681 ggacacacgt tgaagcattt ggagcagcca atacaagcca ctttgcaaac ttttttattt
    16741 acagctccct tatccttgtt tacgcaattc acgtaaattc ttctcgattt aggacctttt
    16801 cgtcttagtt caatgatgtt tttgggacat gcttttacgc aggcgccaca ggctgtgcat
    16861 ttatcttcat caacttcggg caacattgtt tcgggattaa tatggatagc atcgaaagtg
    16921 caagaaactt cgcaatctcc cagtcccaaa caaccccagg aacaaccggt ttcacctcca
    16981 tataaattgt gtacaatcgt gcatgatttt acaccatcgt aactattggt tttttcgcgc
    17041 atttcgcaag ttcctccgca gcgaaccaca gctatggtag gaactactgc cgctgctttc
    17101 tttcccaaaa tatcggccac tttttccata gtagccgtac ctcctaccgg acaagataaa
    17161 ttatctagtg tatcggctgt tacacatgct gctgcaaatg cccggcaacc agggaacccg
    17221 caaccaccac agttggcagc cggtaatact gcttccactt cgtcgatgcg tgcatcttct
    17281 tctacatgaa atttttgggc aataaaatat agaataactg ccgataaagc gccgattcct
    17341 ccaagtgaaa ctactgatgc taatattaaa ttcattgctg atttatttgt gatttgattt
    17401 tactaaagaa ttttttaggt aagtgattct tttttgacgt gaaattcaaa ttttcttttt
    17461 aatttatggt tgaataatga aagtatccca taatatggaa atatggagca cagtgctatg
    17521 atgccggaaa ttgtttcgtt ttgctgaaaa taattggaaa ttattaaggt aagaacaatc
    17581 agcaacaacg ggaaaataaa agccagcaaa acggcttgaa gtcccattga atttcgtcca
    17641 aaaattacta cttgatcacc ttcttttatt gaagaatcgg ttacgactac atcaacaatt
    17701 tttttttcct cttccattac agtacaggca cttttggcat gacacgcact gcatgccgac
    17761 tgctggatga taagtatttg agctttactc ccttcaatat gttgaactat gccgatgtgt
    17821 tctatttgtt gcattcaatt ttttttttgc aatgttgaca ttacaatttg tcggcaaaaa
    17881 tacggattat ttatatttac atcaaaaaaa atcaataaaa aatcgatttt gtagagtcaa
    17941 ccgggaattt gagaagttaa attttttaaa agtatgagta ccctcttaaa tttgattttt
    18001 tcaaaaaagg gaaaaataag attatttgaa aagcagcatt aaattttttt attgtttcga
    18061 tattttacaa taatttgcaa tataaaagtt atttttgtta caaaag
//
LOCUS       DAOSCH010000033        18087 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2151_length_18087_cov_6.582354, whole genome shotgun sequence.
ACCESSION   DAOSCH010000033 DAOSCH010000000
VERSION     DAOSCH010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 18087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18087
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2151_length_18087_cov_6.582354"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1210)
                     /locus_tag="PLZ54_04095"
     CDS             complement(128..1210)
                     /locus_tag="PLZ54_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66146.1"
                     /translation="MKKILYLLLLTFICASCNDMKDKPEPRNFPENTNEKGKMYVLSE
                     GLYHLNNSTLACIDFDERKMDFNFFAVKNGRGLGDTANDMKRYGDRLWIVVCGSSQVE
                     IVHISTGKSIKRLALTNQNDEPRYPRAITFWENKAYVCSFDGSITEIDTASFVMKTNI
                     YCGRNPDGIVAVNGKLYVSNSGGLDFPNYDSTVSVIDLAIYREIKRIKVGQNPYKLQS
                     DSEGDVYVVSRGNNGTIPAVWQRIDSKTDELLQTFADLPVGNFVIQNDTAYMYNFDFI
                     TNNFWFKSFDCKTERIINERLITDETEIERPYSISVHPSNGNIYICDAKNYTKNGTLF
                     CFDKNGRLRYKIPQIGLNPNNLIIVE"
     gene            1986..2462
                     /locus_tag="PLZ54_04100"
     CDS             1986..2462
                     /locus_tag="PLZ54_04100"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOJ66147.1"
                     /translation="MNFYDFEAKKINGENISMQQFKGKTVLVVNTASKCGLTPQFEGL
                     EKMYEKYKDKGLVILGFPCNQFAHQDPGSSSEISEFCVVNYGVTFPMFEKVDVNGKNA
                     HPIFKYLKSQLKGGLFGSKIKWNFTKFVIDKNGNPVKRFAPTVTPEKIEKYIEKIL"
     gene            complement(2642..3862)
                     /locus_tag="PLZ54_04105"
     CDS             complement(2642..3862)
                     /locus_tag="PLZ54_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ66148.1"
                     /translation="MTQSKEKLWTASFISICVANFMLFFAFYLLLPILPLYLIENFQA
                     SKSLVGIILSSYTIAALLIRPMAGFLLDMFQRRPIYLLAYFLFSLCFIGYPLANFLNF
                     FLSLRIVHGLCFGMVSTASNSFVIDIMPSSRRGEGLGYFGIANNLAMSIGPMISLFLH
                     DYSSYQMIFFTSIASSLIGFVVVLTVKAKKTTIPTIKKYKEAIVLDRFFLIKGTHAGI
                     CLTLMGIPYGMLTTFIAIYGKEIGMYSGMGIFFTLMAIGLIFSRFFAGRMVDRGHLLK
                     VITVGTIVCTVSIFVLPALKDLFLLQQNFSMILFYVVAVFLGLGYGMLFPAYNTLFVN
                     LAPHNRRATASSTFLTTWDVGIGLGLIIGGKLGDTHGGLSLSFLTGGLIAFSSLVYFI
                     KITAPHYKKNTIIT"
     gene            complement(3875..4423)
                     /locus_tag="PLZ54_04110"
     CDS             complement(3875..4423)
                     /locus_tag="PLZ54_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOJ66149.1"
                     /translation="MIINERNTRRERLMQAANAMMTAARTAPKGKGYDIIEIAIITDE
                     TIVELSNAMFEYSQQSGLKFITRDAENILKSEVILLIGTRQQNQNLNCGYCGFDTCAE
                     KNSQPDIPCALNTVDVGIAIGSACAVAADLKVDTRVMFSVGRVAKEIGLLPGCSSIFG
                     IPLSCSSKNPFFDRVSKTPAPK"
     gene            complement(4485..5282)
                     /locus_tag="PLZ54_04115"
     CDS             complement(4485..5282)
                     /locus_tag="PLZ54_04115"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005803970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOJ66150.1"
                     /translation="MEIELNQLCLSVCEIARSAGKMIADERKRFDDSKVEYKGLHDLV
                     SYVDKNSEKLIVSQLEKLLPEAGFLAEEGTTSKEGERYNWIIDPLDGTTNFVQGVPVF
                     AVSIGLHEANEMVLGVVYEIGRDECFYAWKGGGAFLNGRAIHVSERDNIQDALLATGF
                     PYSEFSRLNEYMEFLKWAVTNSRGVRRLGSAATDLAYVACGRFEAFWEYNLKAWDVAA
                     GSIIVKEAGGTVTDFSGKNNFLFGKEIVATNQKLKQLFLDKLREMMF"
     gene            5614..7572
                     /gene="gyrB"
                     /locus_tag="PLZ54_04120"
     CDS             5614..7572
                     /gene="gyrB"
                     /locus_tag="PLZ54_04120"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOJ66151.1"
                     /translation="MITEEKLNAIAEYDASSIQVLEGLEAVRKRPAMYIGDIGIKGLH
                     HLVYEVVDNSIDEAMAGHCDDIQVTIHPDNSITVTDNGRGIPVDFHEKEGKSALEVVM
                     TVLHAGGKFDKGSYKVSGGLHGVGVSCVNALSSFMRVEVARNGKIYEQEYERGIPKFP
                     VREIGTTSTTGTRTTFIPDDTIFTEVVYKYDIIADRLRELAFLNAGIRLTLVDERTVE
                     EDGSFRKEVFYSKEGLKEFVRYIDEAREKLIDDIIHIRNEKNGIPVEIAMTYNTSYNE
                     NIHSYVNNINTIEGGTHLAGFRRGLTRTLKKYAEESKMLDKVKIEISGDDFREGLTAV
                     ISIKVAEPQFEGQTKTKLGNNEVMGAVDMAVGEALSAYLEENPKAARMIVEKVILAAT
                     ARNAARKAREMVQRKSPLSGGGLPGKLADCVERDPAKCELFLVEGDSAGGTAKQGRSR
                     QFQAILPLRGKILNVEKAMQHKVLESEEIRNIYTALGVTIGTEEDSKALNISKLRYHK
                     IIIMTDADVDGSHIATLIMTFFFRHMKELIEQGYLYIATPPLYLCKKGKIEEYCWTEQ
                     QRQAFIEKYGNGQEGSIHVQRYKGLGEMNETQLWETTMNPEKRTLRQVTIENAASADH
                     VFSMLMGDDVLPRRQFIEQNATYANIDA"
     gene            7669..8217
                     /locus_tag="PLZ54_04125"
     CDS             7669..8217
                     /locus_tag="PLZ54_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOJ66152.1"
                     /translation="MNVAVFCSSSNQIQPVYKQAAFLLGQFIAESGFTLVYGGSTGGT
                     MDDIALGATEKKGKIIGVIAQPIIRMHRQSTLPTELIVVEDLSERKNKMKQLADVFVA
                     LPGSYGTFDEMMDVIASGVVGEHHKPIAVVNEQHFFDDFIRQINRMKIEQFLPEMENY
                     RPVFVDNINQCKRFLQSLSIRN"
     gene            8231..9553
                     /locus_tag="PLZ54_04130"
     CDS             8231..9553
                     /locus_tag="PLZ54_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66153.1"
                     /translation="MALFGNKFFRKITPTGKFERSENERVQSLQRYNEIARSAELAEY
                     QQLSKVISSPKFQENKKILKNRNYKDTEEYRLSSEYKKLQNSHSLQQYFKTLESKELN
                     DFLKFKETPDYLDLGNRNKVKASEELKKFKQFEHSKEYKNFVRYHDSFVVKEYEELKE
                     KVNSPEFKKSNDFWANPDRWHTTKEYALQRRHHQLRKNPDIIYYENENPERFGIFRSL
                     ELTFEENFDWNTLDSSRWKYGFHYKSPQLIGDHSFANEKQANNSGKNVIVSDGRLKIF
                     TKEEKVTARAWDIQKGFIMKEFDYTSDVIQTSDSFRQKYGMFRAKLRCSGKIHHAFWL
                     GGDEMLPHINIFHFDGKYINVGNASKNEMDGTKIKGIHPSEFYIYSLVWTEKELIWMI
                     NNYEVYRTVRNIPDEELYLALNSFIPEKEKGDKGVLEVDWIKVYQFKQ"
     gene            9590..10402
                     /gene="kdsA"
                     /locus_tag="PLZ54_04135"
                     /pseudo
     CDS             9590..10402
                     /gene="kdsA"
                     /locus_tag="PLZ54_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
     gene            10433..10957
                     /locus_tag="PLZ54_04140"
     CDS             10433..10957
                     /locus_tag="PLZ54_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HOJ66154.1"
                     /translation="MVNQPVEKVQLPQKEKQKIKLLLSDIDGVMTDGGLYYSEFGDQS
                     KKFHVRDGMGLKILQSKGIKVGIVTSEDNKIAEMRYNKLQLDYLYKGRKNGGKLAAAL
                     EICEKEGISLQQTAYIGDDVNCYELLCSVGLAACPADAMELIKSVPGIIKMKAKGGQG
                     CVREFVEYILKNYC"
     gene            10970..11926
                     /locus_tag="PLZ54_04145"
     CDS             10970..11926
                     /locus_tag="PLZ54_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOJ66155.1"
                     /translation="MDFVKRSKEIFEQEISELQKLAEKIGPEINRAVELIYNCKGKLV
                     ILGIGKTGIIGHKIASSLASTGTQTIFINAAEAMHGDLGMIDRNDIALLISNSGSSPE
                     IVNLITPLKKIGCTIIAMTGNPKSPLAREASLVINTGIEREACPLGLAPTTSTTATLV
                     MGDALTICLMERRNFKAENYALYHPGGALGRQLLARVKDQMNHDIPKVYTHTLFKDVI
                     YEVSNKRLGMTMVYDDNENAVGIITDGDIRRAIQRFDDLKNLTAQEFMTHGFKKIAQN
                     EMLSEALEIMDENNITNLAVMENDSSSTVVGILSIHDIIDFK"
     gene            11939..12784
                     /locus_tag="PLZ54_04150"
     CDS             11939..12784
                     /locus_tag="PLZ54_04150"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ66156.1"
                     /translation="MFKKIYIEITNSCNFHCSFCFTTSQPPRFMPVKDFHTIVRKIKP
                     FTNYIYLHVLGEPFLHPHIEDILNVAEQEGMNVNITTNGSLLNNRKKLLLNHSIRQLN
                     ISLHDAEENIPSIHWESFFENIFNIAMEISAKTYVNLRLWNIDNPVSGKFNSLCKEKI
                     VEKFQLSPDELTQPEKGNSIKLANHIFLQQSPKFQWPTENGEPLFEHKSCYALRDHIA
                     ILAGGEVVPCCLDAGGSLKLGNIFTEELSDILMCERAKKMKQGFRQHKITEPFCKTCG
                     FSVAF"
     gene            complement(12806..13864)
                     /locus_tag="PLZ54_04155"
     CDS             complement(12806..13864)
                     /locus_tag="PLZ54_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HOJ66157.1"
                     /translation="MTAELANEAIQLLRQMIAIPSFSKEEKEIADFLEQYLTERGYVV
                     FRHLNNIWLQSPGFDVSRPTILLNSHLDTVKPGSSWIRNPFEASLKNGKLFGLGSNDA
                     GASLVSLLQVFFYLTQRPQPYNLIFAATAEEEISGKNGIEIMLQEFPKVDFAIVGEPT
                     GMNMAVAERGLMVIDATVTGKSGHAAREEGENAIYKALDAINWFKNYRFAKVSTLLGP
                     VKMTVTQINGGIQHNMIPDKCTFVVDVRSNELYSNEEILEIIRENVDCRIEPRSVRLC
                     STATQPDHPIIKRAQQMNLTLYGSPTLSDQALMPFPSVKIGPGDSARSHTADEYVLVE
                     EIEKGIEVYIQLLDGLQI"
     gene            complement(13926..14738)
                     /gene="argB"
                     /locus_tag="PLZ54_04160"
     CDS             complement(13926..14738)
                     /gene="argB"
                     /locus_tag="PLZ54_04160"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HOJ66158.1"
                     /translation="MNSDKSPNIDMFQKKEKLTIIKVGGKIVEESDSLISLLNDFSKI
                     DGYKVLVHGGGRSATALATRLGLETKMIEGRRVTDAEMLKVVTMIYAGWVNKQIVAGL
                     QALGLNALGLTGADLNYMRSEKRSPYPIDYGFVGDVKEVNVSLLTDLIRKEVVPVLAP
                     LTHDKCGNLLNTNADTIAAEAAKAFSSFFEVTLIYCFEKKGVLMNEKDEDSVISSLTP
                     ELFDKFVSQKVIQGGMIPKMENSFNALKSGVSKVIITRADAILTHSGTTITL"
     gene            complement(14810..15334)
                     /locus_tag="PLZ54_04165"
     CDS             complement(14810..15334)
                     /locus_tag="PLZ54_04165"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOJ66159.1"
                     /translation="MDVEQVIASIRNIPDFPKKGVLFKDITTAMKDAEVLRFISDELY
                     EYYKDKGITKVVGLESRGFMLGSILAYKLHAGFVLIRKAGKLPAATYRVDYELEYGVD
                     SLEIHKDAIEKDDVVLLHDDLLATGGSANAALTLLDNFKPKAIYVSFLIELKDLKGRD
                     RLKGVQDVHSLIQF"
     gene            complement(15398..16351)
                     /locus_tag="PLZ54_04170"
     CDS             complement(15398..16351)
                     /locus_tag="PLZ54_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine carbamoyltransferase"
                     /protein_id="HOJ66160.1"
                     /translation="MKTFIRVQDLGNLQEALQEAFEVKKNRFGYQHLGKNKTLLMVFF
                     NSSLRTRLSTQKAGMNLGMNTIVLDINQGAWKLETERGVVMDGDKTEHILEAIPVMGN
                     YCDLIGVRSFAQFENKAYDYQETILQQFIQYSGRPVFSMESATVHPLQAFADLITIEE
                     YRKKPHPKVVMSWAPHPKALPQAVPNSFADFMNEADVEFVITHPKGYELAPEFVRNAR
                     IEYDQMKAFEGADFVYAKNWAAYQDPNYGKIICTDRSWTVSSRQMAVTNNAYFMHCLP
                     VRRNMIVTDEVIESLQSLVIPEAANREISAEVVIKRMLESL"
     gene            17122..>18087
                     /locus_tag="PLZ54_04175"
     CDS             17122..>18087
                     /locus_tag="PLZ54_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66161.1"
                     /translation="MKKNNYFLLAVFTAVVAFTSQQAKAFPPYIYEGFDYEIGATPLN
                     YNPLVPGTWGAVHMWKPASSWNGYGGSFSNTFPWGVTDVSPMVVPSLVQTGKYFNGNG
                     EWVSCFLREINTDWNSPYWDYMDESKLGKSGTTLWFSVILRPQKNKKEAALGLCKAYN
                     EQSVMKIGAFGGNYWGIRVNNDAEQALSTVPIVNDEAKFLVCKVEYTDVTGGTITLYI
                     DPTPGVEPSNAAATLTTTSDIRFKSVQLLTHYSGGCMAADEIRIATTYDEVAPSTGPS
                     TGLVQDMKKDIRVYSRDGKVVADLTMLKGLSTVSVIDTKGAVVKTI"
BASE COUNT         5864 a         3549 c         3452 g         5222 t
ORIGIN      
        1 gactaaaaaa aataatccta atatcatatt gttctaatgg acaatttggg tttatgtctc
       61 acgaaatatt ggagaacaag gcaaattgtt tattcggcga gaaatgaaaa aatacatttt
      121 ttgagcatta ctcaacaatt atcaggttat tcggattcaa cccaatttgc gggattttgt
      181 atcttaatct cccgtttttg tcgaagcaaa ataaagtacc attcttagta tagttctttg
      241 catcgcaaat ataaatattc ccatttgaag gatgaacaga tatggaataa ggacgttcaa
      301 tttcagtttc atctgtaata agccgttcgt taatgatgcg ttcggttttg cagtcgaaac
      361 tcttaaacca gaagttgttg gttataaagt cgaaattgta catataagct gtatcgttct
      421 ggattacaaa attccccaca ggcaagtccg cgaaagtctg aagtagctca tccgttttgc
      481 tgtcgatgcg ttgccatacg gccgggattg ttccgttatt tcctcgagaa accacgtaaa
      541 cgtctccttc gctgtccgat tgaagtttat atgggttctg acctactttt attcttttta
      601 tttcacgata tatcgccaaa tcaattaccg aaacagtgct gtcgtaattt ggaaaatcga
      661 gaccgccgga attggatacg tatagtttcc cgtttactgc aacaattcca tcaggatttc
      721 gaccacagta aatattcgtt ttcatgacaa aactggcagt gtcgatttcc gttattgaac
      781 catcaaaaga acacacataa gctttattct cccagaaagt aatggcacgg ggataacgtg
      841 gttcgtcatt ttggttggtt aatgccagtc tttttatcga ttttcccgtt gaaatatgta
      901 caatttccac ttgagaagag ccacaaacca ctatccacaa acggtctcca taccttttca
      961 tatcgtttgc agtgtcgcca agacctctgc catttttaac tgcaaaaaaa ttaaaatcca
     1021 tttttctttc atcaaaatcg atgcaggcaa gggtgctgtt gttcagatga tacaatcctt
     1081 cggataaaac atacattttc cccttttcat tcgtattttc aggaaaattt cgaggttcgg
     1141 gcttgtcctt catatcgttg catgaggcac aaataaaagt cagtaagagt aaatagagta
     1201 tttttttcat ctttttccag aactttgagt ctgcaaaatt acatttttcc agctaaaaag
     1261 taccattagt ccacaaatag atcgattata caagcactaa tcacttgttg tcctgtgctg
     1321 tcgcacaatt ttatttgata aactcttctt tgttttctcg aaaaaccact tgatttgcca
     1381 attcgacttc cagaagcatt atacaaatca tctcagattg ctttacatat ttgacgtttc
     1441 tctccgtcgt acatgcaatc accatttaac ttttcaattt accttccgta gcatcatata
     1501 ttttacatgc atcaaaccaa aaaagtacgt gatggatttc tatatcaaaa ttatttattt
     1561 catcagaata taataaggtc tgatggaacc cacatgtttg acatattata ttcattcgtg
     1621 tttgtgtatc taatacatgt gggtttcatt tttattttta ttatttacaa ataatgttgt
     1681 ccgataaaaa taaaggagca aatagtgtcg ctcctacgga gctctaatga tatttgtaac
     1741 ttattgacta ccataatatc gtcccgatgg gacttaagag aaagctgcat tgcagcgaaa
     1801 ttatggtaga aaaattcaac ccacaaaatc taagccccat taggggcgat attatagtaa
     1861 gacattttct atattgattt tcactaattt ttataaatgt ttaccggaca acaatgattt
     1921 gcaaacaaaa aagcaacaac tttgttatca atacaaataa agattttatc aactaaaaaa
     1981 acaatatgaa tttttacgat ttcgaagcaa aaaaaatcaa cggtgagaat atttccatgc
     2041 agcaatttaa aggaaaaacc gtgttggtag ttaacactgc aagtaaatgt ggattgacac
     2101 ctcaatttga ggggcttgaa aaaatgtatg aaaaatacaa ggataaagga ttggttattt
     2161 tgggctttcc atgcaatcag tttgcgcatc aggacccggg ttcatcaagc gaaatcagtg
     2221 aattttgcgt tgtaaattat ggcgtaacct tcccgatgtt tgaaaaagta gatgtaaatg
     2281 gaaaaaacgc ccacccaatt tttaaatatc tcaaatcgca attgaaaggt ggattgtttg
     2341 gaagtaaaat taaatggaac ttcacaaaat ttgtcatcga caaaaacggc aatccagtaa
     2401 aaagatttgc acctaccgta actccggaaa aaatagaaaa atatatagaa aaaattttat
     2461 aatgtgttga aaaacaaatt tataattaaa taaaatttat tttaaaaaga aagaaggtat
     2521 tccaaaaaat actttctttt ttattttaag cagccgcttg aggagtaagt tttgattggg
     2581 aattgccttt cattatattc cttgaatatg cgaataataa aaaaacagga atttaaatcc
     2641 atcatgtaat gatggtattt ttcttataat gaggggctgt aatttttata aaatagacca
     2701 gcgatgaaaa agcaatcaat cccccagtta aaaacgaaag cgaaagtccg ccatgcgtat
     2761 cgccaagttt tcctccaata atcaaaccca gtcctattcc tacgtcccaa gtagtcagga
     2821 atgtggaact tgccgttgcc cggcgattgt gaggcgccaa gttgacaaac aaagtattgt
     2881 aagcgggaaa tagcattcca taacccaaac cgagaaaaac ggccaccaca taaaacaaaa
     2941 tcatggaaaa attttgctga agaagaaaca aatctttcaa tgcaggcaaa acaaaaatgg
     3001 aaaccgtaca aacaattgtt ccaacggtaa taactttcaa taaatgacct ctatccacca
     3061 tcctgccggc aaaaaatctc gaaaaaatca gtccaatggc catcaaagta aaaaagatgc
     3121 ccatgcccga atacataccg atttctttcc cgtaaatggc tataaaagtg gtcaacatgc
     3181 cataaggaat tcccattaat gtcagacaga tgccggcatg agttccctta atgaggaaga
     3241 aacggtccaa cacaatcgct tccttgtatt tttttatagt aggaattgtg gttttcttgg
     3301 cttttacggt taaaacaaca acaaagccta tcaatgagct ggcaatggat gtaaagaaaa
     3361 tcatttgata agaagaataa tcgtgtaaaa acaagctaat cattggccca atagacatgg
     3421 ccaaattgtt ggcaatgcca aaataaccca atccttctcc tctcctcgaa gaaggcataa
     3481 tatcaataac aaagctatta cttgctgtgg ataccatacc gaagcacaat ccgtgaacaa
     3541 tacgcaggga caaaaagaaa ttcaaaaaat tggccagcgg ataacctata aaacagagcg
     3601 aaaaaagaaa ataagccaac agataaatgg ggcgccgttg aaacatatcc agcaaaaaac
     3661 cggccatagg tcgaatcagc aaagctgcta tcgtataact ggacaagata atgccaacaa
     3721 gcgatttgct ggcttggaaa ttttctatca aataaagtgg aagtatgggc agcaagaggt
     3781 aaaatgcaaa aaacagcata aagttggcaa cacaaatgct gataaaactt gccgtccaga
     3841 gtttctcttt ggactgagtc attattttag aaaattattt aggagccggt gttttggaaa
     3901 cacgatcgaa aaatggattt ttggacgaac aactgagcgg aattccgaaa atggaactgc
     3961 atccgggcaa caaacctatt tctttcgcca ctcttccaac ggaaaacata acacgtgtat
     4021 ccactttcag gtcagcagct acagcacaag ccgagccaat agcaatacct acatctaccg
     4081 tgttcaaagc acagggaatg tcaggttgac tattcttttc ggcacatgta tcgaacccac
     4141 agtaaccaca attcaaattc tgattttgtt gtcgggtgcc aattaataaa attacttccg
     4201 atttcagaat attttcggca tcgcgcgtaa taaatttcaa tccggattgt tgactgtact
     4261 cgaacatggc atttgacaat tccacaatag tttcatcagt aatgatcgct atttcgatta
     4321 tatcgtatcc tttacctttg ggagccgtac gggcagctgt catcatggca ttggccgctt
     4381 gcattaagcg ttcgcgacgt gtattgcgtt cattgataat cataataaga gttttttaca
     4441 gattccttta ttgctataac caaactaaaa gtttagcagt gcatttaaaa catcatttct
     4501 ctcagcttgt caagaaaaag ctgtttcagt ttttgattgg tagctacgat ctccttccca
     4561 aacaaaaaat tgttttttcc tgaaaaatcg gtaactgttc cgcctgcttc cttcacaata
     4621 attgaaccag cagctacatc ccaagcctta agattgtatt cccaaaaagc ttcgaatcgg
     4681 ccgcaagcta cataagccaa atcagtggca gccgaaccga gccggcgaac cccacgtgaa
     4741 tttgttacgg cccactttag aaattccata tattcgttta gccggctgaa ctccgaataa
     4801 gggaaaccgg tagcgagcaa tgcatcctga atattatcgc gttcagaaac atggatggct
     4861 ctaccgttga gaaaagctcc acctcctttc caagcataaa aacattcatc tcttcctatt
     4921 tcgtaaacca ccccaagaac catttcattg gcttcatgca agccaatgct tacggcaaaa
     4981 acaggaacac cctgaacaaa attggtcgta ccatccaacg gatcaataat ccagttgtaa
     5041 cgctctcctt ctttcgatgt tgtaccttct tccgctaaaa aaccggcttc aggcaaaagt
     5101 ttttcaagtt ggctgacaat cagcttttcc gaatttttgt caacatagct caccaaatca
     5161 tgtaaacctt tgtattccac tttgctgtca tcaaatcttt tccgttcgtc ggcgatcatc
     5221 tttcccgccg atcgggcaat ttcacatacc gaaaggcaaa gttgattgag ttctatctcc
     5281 atgtcttttt ttaattaaaa atcgactgca aagttagcct ttttttgaga aacatggaca
     5341 aaaatattga ctgctgtcga caacaagcat tccatttttc gtgataaatt tattcaataa
     5401 tcgagcacaa aaagcatgtc gttaaaatgt tcaaaaaact ttattaaatc gggattttaa
     5461 aattctgaaa tagtgatgag atgttgtata tttcgctcaa aaaacgaggt tatctcaaat
     5521 atttttatta aatttgtatg ttttttcaaa acactttttt cggttgaaaa atggcttatt
     5581 aatagagaaa aaaataaaat taactatata gaaatgatta cagaagaaaa gttaaatgct
     5641 attgccgaat atgatgccag cagcattcaa gtcctcgaag gactcgaagc tgtacgcaaa
     5701 cgtcctgcca tgtatattgg ggatataggc atcaaaggat tacatcattt ggtttatgaa
     5761 gtggtagata attctatcga cgaagcgatg gcaggacatt gtgatgatat tcaagttacc
     5821 atccatccgg ataattccat tactgtaact gataacggac gcggtattcc ggtcgatttt
     5881 cacgaaaaag aaggaaaatc ggcattggaa gttgtaatga cggtgcttca cgccggagga
     5941 aaatttgaca agggaagtta caaagtttca ggaggtttgc atggggtggg agtgtcttgt
     6001 gtaaatgcgc tttcgtcgtt tatgcgcgtg gaagttgcca gaaacggtaa aatttacgaa
     6061 caggaatatg aacgcggtat tcccaaattc cctgtaagag aaattggaac tacttctaca
     6121 acaggcacac gcactacttt catccctgat gatacgattt tcacggaagt cgtttacaaa
     6181 tacgacatca tcgccgaccg ccttcgcgaa ttggctttct tgaatgccgg tattcgattg
     6241 acacttgtag atgagagaac agtggaagaa gatggaagtt tcaggaaaga agttttctat
     6301 tccaaagaag gactcaagga gtttgtcagg tacatagacg aagctcgtga aaaactgatt
     6361 gatgacatta ttcacatcag aaatgaaaaa aatggcattc cagtagaaat cgccatgact
     6421 tacaatactt cctacaatga aaacattcac tcttatgtga acaacatcaa caccattgag
     6481 ggaggaacgc atttggccgg tttccgaaga ggactgacca gaacgctgaa aaaatatgcc
     6541 gaagaaagca aaatgctcga taaggttaaa attgaaatca gtggcgatga ctttcgtgaa
     6601 ggactgacgg ctgtcatttc gataaaagtt gcagaacctc agtttgaagg acaaacaaaa
     6661 accaagcttg gcaacaacga agtaatggga gcagtggata tggctgtcgg agaagctttg
     6721 agtgcctatc tcgaagaaaa tccaaaagca gcccgaatga ttgttgaaaa agtgattctc
     6781 gctgccacag ctcgaaatgc agctcgcaaa gcacgcgaaa tggttcagcg caaatcgccc
     6841 ctttccggtg gaggactacc cggaaaactt gccgactgtg tagaaagaga tcctgccaaa
     6901 tgtgaattgt tccttgtcga aggagattcg gccggcggaa cagcaaaaca gggacgtagc
     6961 cgtcaattcc aagctatttt gccgctgagg ggaaaaattt tgaatgtgga aaaagccatg
     7021 caacacaaag tgctggaaag tgaagaaata cgcaatatat atactgcttt gggcgttact
     7081 ataggaacag aagaagacag caaggctttg aacatcagta aacttcgata tcataaaatc
     7141 ataatcatga ccgatgctga tgtggatggg agccatattg ccacgttgat tatgaccttt
     7201 ttcttccgac atatgaaaga actgatcgaa caaggttatc tatatatcgc tacacctcct
     7261 ctctaccttt gcaaaaaagg aaaaatagaa gagtattgct ggacagaaca gcaacgtcag
     7321 gcttttatag aaaaatacgg aaatggtcag gaaggttcca tacatgttca gcgttacaaa
     7381 ggtttgggtg aaatgaacga aacgcaactt tgggaaacaa ccatgaatcc tgaaaaacgt
     7441 acacttcggc aggtaaccat cgaaaatgcc gcttccgccg atcatgtttt ttcgatgctt
     7501 atgggagacg acgtgttgcc gcgacgccag tttatcgaac aaaatgccac ttacgccaac
     7561 attgacgcat aacatgcttt tcaaaaattt ccttccactt taaaacactt aaaagtggaa
     7621 ggaaaaattt atataaacaa gctgtgagcg cttgaaaaaa gaaattaaat gaacgttgcc
     7681 gtattttgtt cttccagtaa tcaaattcaa cctgtttaca aacaagctgc ttttctgctg
     7741 ggccaattta ttgccgaaag cgggtttact cttgtatatg gaggttccac aggaggaaca
     7801 atggatgaca tagctttggg agctaccgaa aaaaaaggaa aaattattgg cgtcatcgct
     7861 cagcctatta tcagaatgca tcgccagagt acattaccca cagaactcat cgttgtagaa
     7921 gacttaagcg agagaaaaaa caaaatgaaa caattggctg atgttttcgt tgctctaccg
     7981 ggaagttatg gaactttcga cgaaatgatg gatgtaattg cttccggtgt ggttggagaa
     8041 catcataaac cgatagctgt agtaaatgaa caacatttct ttgatgattt tatcaggcaa
     8101 ataaacagaa tgaaaatcga acaatttttg cctgagatgg aaaattatcg acctgttttt
     8161 gttgataaca tcaatcaatg caagagattt ctccaatcac tttcaataag aaactgaaat
     8221 acaaaaaaat atggctctat ttgggaataa gttttttagg aaaatcactc ctaccggtaa
     8281 atttgaaaga agtgaaaacg agcgtgttca atcgttgcaa cgttacaacg aaatagcaag
     8341 atcggcagaa cttgcagaat accagcaatt atccaaagtc attagttctc caaaatttca
     8401 ggaaaacaaa aaaattctga aaaacaggaa ttacaaggat acggaagaat atcgactgag
     8461 ttcagaatac aaaaaacttc aaaattcgca ttcactgcag caatacttca aaacactgga
     8521 atcgaaagaa ttgaacgatt ttttgaaatt caaggaaact cctgattatc tcgatttggg
     8581 caaccgaaac aaagtaaaag cttcggaaga attaaaaaaa ttcaaacagt ttgaacattc
     8641 taaggaatac aaaaattttg ttcgttatca tgactctttc gtggtaaaag aatacgaaga
     8701 gctgaaagaa aaagtaaatt cacccgaatt caaaaaatcg aacgattttt gggccaaccc
     8761 cgacagatgg cacaccacaa aagaatatgc ccttcagcgt cgtcatcacc aactccgaaa
     8821 aaatccggat attatttatt acgaaaacga aaatcccgaa agattcggaa tatttcgttc
     8881 actggaactg acatttgaag aaaatttcga ttggaatacg cttgacagtt ctcggtggaa
     8941 gtatggtttt cattataaaa gccctcagct tatcggcgat cactcttttg ccaacgaaaa
     9001 gcaagcaaac aattcaggta aaaatgtaat agtttctgat gggcgtttaa aaattttcac
     9061 caaagaagaa aaagttactg ctcgcgcttg ggatattcag aaaggattta taatgaagga
     9121 attcgattac acttccgatg ttattcagac gtccgactct ttccgccaaa aatacggaat
     9181 gttcagggca aaacttcgtt gcagcgggaa gatacatcat gctttctggc tgggtggtga
     9241 tgaaatgttg ccacacatca atatctttca tttcgacgga aaatacatca atgtcggtaa
     9301 tgcttcaaaa aatgaaatgg atggaaccaa aatcaaggga attcatcctt ccgaatttta
     9361 tatttacagt ttagtgtgga cagaaaagga actgatttgg atgattaaca attacgaagt
     9421 gtaccgaacg gtaagaaata taccggatga agagttgtat ctggcactca attcatttat
     9481 tcccgaaaag gaaaagggcg ataaaggtgt tttggaagtt gactggataa aagtttatca
     9541 atttaaacaa taatcttcct ttttttaaaa aaatcaaact tacggtttca tgattgaaac
     9601 aaaaaaaata aatgttcttc atcagtttga tataggcgat ggcggtcgct ttgtgctcat
     9661 agccgggcct tgcgccattg aatcggaagc catgactatg gaagtggccg gaacgctgaa
     9721 agaaatctgt cgggaattga atatccattt gatttttaaa tcttctttcg acaaagccaa
     9781 tcggacttca ttgaaatcgg ctcgtggcgt tggcatggag cgcggacttg aaattttaca
     9841 aaaagtgaag aatgaatttc aacttcccgt actgactgat gttcacgaaa catggcaatg
     9901 ccaacctgtt gccgacgttg tggatgtgct gcaaattcct gcttttcttt ctcgacaaac
     9961 cgaccttttg acagctgccg ccaaaaccgg gaaaattgta aacgtaaaaa aaggtcagtt
    10021 catggccccc tgggacatga aaaatgtggt ggataaaatg ttggaagcag gaaacgacaa
    10081 aattctgctt tgtgagcgcg gttcttcctt tggatacaat aatttggtgg tggatatgac
    10141 cggattggta gaaatgcgaa aatatggttt ccctatagtt ttcgacgcca ctcattccgt
    10201 acaaaagccc ggagggcagg gaaattcaac aggaggaaac agagaaatgg ttccttattt
    10261 gatgcgggcg gcactggctg tcggcgtgga tgcagttttt gccgaagttc atcctcaacc
    10321 cgattatgca atttccgatg gcccgaatca actttatctt tccgatgttc gtaacatctt
    10381 acagcaagcc attttaattg acaatgttac gaaaaacctt tccgaaaaag aaatggtgaa
    10441 tcaaccagtt gaaaaagtac agttgcctca gaaagaaaaa caaaaaatca agttgttgct
    10501 ctccgatata gatggagtaa tgacagatgg aggcttgtat tattctgaat tcggcgacca
    10561 atcaaaaaaa tttcatgtac gtgacggcat gggtttaaaa attttacagt cgaaaggaat
    10621 aaaagttggc attgttactt ccgaagacaa caaaattgca gaaatgcgat acaacaaact
    10681 tcagctcgat tacctgtaca agggcagaaa aaacggagga aaattggctg cagcattaga
    10741 gatttgcgaa aaagaaggca ttagtttgca gcaaacagcc tatattggcg atgatgtgaa
    10801 ttgttacgaa ttgctatgct cggttggttt ggctgcatgt cctgccgatg caatggaact
    10861 gataaaaagc gtgccgggaa ttattaaaat gaaagcaaaa ggtggacaag gctgtgtgcg
    10921 ggaatttgtg gaatatattc taaaaaatta ctgttgatat attaaaatca tggattttgt
    10981 aaaaaggagt aaagaaattt ttgaacagga aatatcggaa cttcaaaaat tggccgaaaa
    11041 aattggacct gaaattaacc gggctgtaga gttgatttac aactgcaagg gaaaactggt
    11101 aattttagga ataggaaaaa cgggtatcat tggacataaa attgcttctt cactggcctc
    11161 taccggtacg caaaccattt tcatcaatgc ggctgaagcc atgcacggcg atttgggaat
    11221 gattgacaga aacgacatcg ctcttttgat ttccaacagc ggaagttctc cggaaattgt
    11281 caacctgatt actccactga aaaaaatcgg ctgcaccatt attgccatga ccggaaaccc
    11341 gaaatctcca ctggctcgag aagcttcatt ggtaatcaat accggcattg agcgcgaagc
    11401 ctgtcctttg ggactggcac ccacaacttc tactaccgca acgctggtta tgggcgatgc
    11461 gttgacaata tgcttgatgg aaagacgaaa cttcaaggca gaaaattatg ctctttatca
    11521 tcccggagga gcgctgggac ggcaattgct ggcacgggtt aaagaccaaa tgaaccacga
    11581 tattccaaaa gtttataccc atactctttt taaggatgta atttatgaag tgagcaacaa
    11641 acgactggga atgacaatgg tatatgatga caacgaaaat gccgtaggca tcattaccga
    11701 tggagatatt cgacgtgcta ttcaacgatt cgatgattta aagaatctga ctgctcagga
    11761 atttatgaca catggattta agaaaattgc tcagaatgaa atgcttagtg aggcattgga
    11821 aattatggac gaaaacaaca ttaccaatct ggcagttatg gaaaatgact cttcctcaac
    11881 tgtagtcggc attttgtcaa ttcatgacat tatcgatttc aaatgaagtg aacaacccat
    11941 gttcaaaaaa atttacattg aaatcaccaa cagctgcaat tttcactgca gcttttgttt
    12001 tactacatct caaccaccga gatttatgcc ggtgaaagat tttcacacaa tcgtccggaa
    12061 aattaaacct tttaccaatt atatttatct gcatgtgttg ggagaacctt ttcttcaccc
    12121 tcatattgaa gatattctga atgttgccga acaggaagga atgaatgtca atatcaccac
    12181 aaacggctct ttactcaata atcggaagaa attacttttg aaccattcca ttcgtcaact
    12241 gaacatttcg ctgcatgatg ccgaagaaaa cattccttcc attcattggg aaagcttttt
    12301 cgaaaatatt ttcaacatcg caatggagat ttcggccaaa acttatgtca atttgcgttt
    12361 gtggaacatt gataatcctg taagcggaaa attcaacagc ttgtgtaagg aaaaaatcgt
    12421 cgaaaaattt caactttcac ccgatgaatt aacacagcct gaaaaaggaa acagcataaa
    12481 gctcgccaat catatttttc tgcagcaatc gcctaaattt caatggccaa cagaaaacgg
    12541 agaacctctc tttgaacaca aatcctgtta tgctttacgt gatcacatcg ccattctggc
    12601 cggaggagaa gttgttcctt gttgccttga tgcaggcgga tcgctgaaac tgggaaatat
    12661 ttttacggaa gaactttcgg acatccttat gtgcgaaaga gctaaaaaaa tgaaacaggg
    12721 ctttcggcaa cacaaaatta ctgagccatt ttgtaaaact tgcgggttta gcgttgcttt
    12781 ttgaagtgca ttttcagcaa atttctcaaa tttgcaaacc gtccagcaat tgaatataaa
    12841 cctcaattcc tttctcgatt tcttctacaa gaacatactc atccgccgta tgggaacgag
    12901 cggaatcgcc gggaccaatt ttaacgcttg gaaacggcat taatgcctga tcggaaagcg
    12961 ttggagaacc gtataacgta aggttcattt gctgcgctct cttaataatg ggatgatccg
    13021 gctgagtggc agttgaacac agtcgaacag aacgaggttc aatacggcaa tcgacatttt
    13081 cacggataat ttccagaatt tcttcattgg aataaagttc gttgcttctt acgtcgacta
    13141 caaacgtaca cttatcggga atcatattgt gttgaatgcc gccatttatc tgggttaccg
    13201 tcatcttcac cggcccaaga agcgtagaaa ctttagcaaa acgataattt ttaaaccaat
    13261 tgatggcatc caacgccttg taaatagcat tttcaccttc ttcacgcgcc gcatgacccg
    13321 attttcccgt taccgttgcg tctatcacca ttaatcctct ttcggcaaca gccatattca
    13381 ttcctgtagg ttcaccaaca atggcaaaat caacctttgg aaattcctgc aacatgattt
    13441 caatgccgtt tttccccgaa atttcttctt cggccgttgc agcaaaaatc aaattatagg
    13501 gttgaggtcg ctgcgtcaga tagaaaaaaa cttgcagcaa acttaccaaa ctggcaccgg
    13561 catcattact tcccaatcca aacaattttc catttttcag agaagcttca aagggattgc
    13621 gaatccaact gcttccgggt tttacggtat ccaagtggga attgagcagg atagtcggcc
    13681 gagaaacatc aaaacccgga ctttgcaacc aaatgttgtt taaatgtcgg aaaacgacat
    13741 atccacgttc tgtcagatat tgttccagaa aatcggctat ctctttttct tccttgctga
    13801 aagaaggaat tgctatcatt tgccgcaaaa gttgaatagc ttcatttgca agttctgctg
    13861 tcattttcgt tgtaatatga tgagaaataa aaaaatcaat tcctcaacaa gaaaatttaa
    13921 agtttttata aagttatcgt tgttcccgaa tgcgtgagga tagcgtcggc tcgtgtaata
    13981 atcacctttg aaactccgga cttcaaagca ttgaaagaat tttccatttt gggaatcatt
    14041 cctccctgaa tgactttttg agacacgaat ttatcgaaaa gttccggcgt taaagaagaa
    14101 attacactat cttcgtcttt ttcattcatt aaaacgcctt ttttctcgaa acaataaata
    14161 agtgtaactt caaaaaaaga agaaaaagct tttgcagctt cggcggcaat cgtatcggca
    14221 ttggtattca aaagattacc gcatttatcg tgagtgagcg gagccaacac cggcaccact
    14281 tctttcctga tcaaatcggt taaaaggctc acatttactt ccttcacatc gccaacaaat
    14341 ccataatcta taggataggg cgaccttttt tcggaacgca tgtaattcaa atcggcaccg
    14401 gtaagaccca aagcattcaa tcccaaagcc tgcagacctg ccacaatttg cttgttgacc
    14461 catccggcat atatcatggt aaccaccttc aacatttccg catccgtaac ccgccttccc
    14521 tcaatcattt tggtttccag tcccaagcga gtagccagag ctgttgccga tcgccctcca
    14581 ccatgtacca gtactttgta accatcaatt tttgaaaagt cgttcaacag ggaaattaaa
    14641 ctatcggatt cttccacaat ttttccgcct accttgatga tggtaagttt ttcctttttc
    14701 tgaaacatat ctatgttggg cgatttatcg ctattcattt ctaattcgag attatggagc
    14761 tattacttcg atattatgtc gaaaaaacct atatctttta ttgaaaaaat caaaactgaa
    14821 tgagagaatg cacatcctgc acacctttga ggcgatctct tcccttcaaa tctttcagtt
    14881 cgatgagaaa gctcacataa atagcctttg gtttgaaatt gtcgagcaac gtcagcgcag
    14941 cattcgcact tccaccggtg gcgagcaaat cgtcgtgaag gagaacaaca tcgtcctttt
    15001 ctatggcatc tttgtgaatt tcgagcgaat ctactccata ttcgagttca taatccactc
    15061 tataagtagc ggccggcaat ttccctgctt tacgaatcag tacaaatccg gcatgaagtt
    15121 tatacgccag aatgcttcca agcatgaatc cccggctttc caaaccaaca actttcgtta
    15181 tccccttgtc cttgtaatac tcgtatagct catcggaaat aaatcttaat acttcggcat
    15241 ccttcatggc cgtagtaata tctttaaaaa gaactccttt tttagggaaa tcgggaatat
    15301 tcctgatact tgcaataact tgttctacat ccatagtttt aaacccacac gggcaacaaa
    15361 attaatttaa ataattgata attaaagcaa taaaattcta taaactttca agcattcgtt
    15421 tgataacgac ttctgctgag atttcgcggt tggcagcttc gggaataacc aacgactgta
    15481 gactttcgat cacctcatcg gttacaatca tattccgccg cacaggcaga caatgcataa
    15541 aataagcatt gttggtaacg gccatttgtc ggctgctgac ggtccagctt ctgtcagtgc
    15601 agataatttt tccgtagttc ggatcttgat aggcagccca atttttagca taaacaaaat
    15661 ctgctccttc gaaagctttc atctgatcgt attctatacg tgcatttcgc acaaattcgg
    15721 gagcgagttc ataacctttg ggatgagtaa taacaaattc cacatcggct tcgttcataa
    15781 aatcggcaaa agaattgggt acagcctgag gcagagcttt gggatggggt gcccacgaca
    15841 tgaccacttt gggatgcggt tttttgcgat attcctcaat ggtaatgaga tcggcaaaag
    15901 cctgcaatgg atgtaccgtt gcactttcca tgctgaacac aggtcttcca gaatattgaa
    15961 taaactgctg aagaatggtt tcctgataat cgtacgcttt attttcaaac tgcgcaaaag
    16021 aacgaacgcc aattaagtcg caataattgc ccatcaccgg aattgcttcc agaatatgct
    16081 cggttttatc accatccatc accacgccac gttcggtttc aagtttccaa gctccctgat
    16141 ttatatccag cactatggtg ttcattccca gattcattcc tgctttttgg gtgctcagac
    16201 gcgttctcaa actcgaatta aaaaaaacca tcaacaaagt tttgtttttc cccagatgct
    16261 ggtacccgaa acggttcttc ttcacttcaa aagcttcttg taaagcttcc tgcaaatttc
    16321 ccaaatcttg taccctgata aaagttttca tttctactat ggttatttaa ctgaaatttg
    16381 ttaaggttta atagtttcat cagaatataa tttattttta atgctctgat ggaacccaca
    16441 tgtctgaata ttatattcat tggtgtttat gtatgtaata catacgggtt tcagaaaaaa
    16501 ttaagtattt aaaagtattt tctaaaatta tcaataaaga aacagaccaa atgatgtata
    16561 gaagaaaaat gtttgcattt gattacaaaa gattgttaat cttttggttg gaaaatctgt
    16621 aaaccacaat ttgttttctc tgctgcacaa ctcaatttat ctcaagtcaa tatttattgg
    16681 caaaattagt tattatacaa ataatctcaa atgttttatt atttattttt tgtaaactat
    16741 ataatctatt cttagagttt tattcataac tgatagtttt ctaactgtat taaatattcc
    16801 gagagtcatt acaatccaaa ttttaaaccg atttttgttt ggttatctca taatatattt
    16861 tcatgcaata ttagcatgta tttcgaatat gatgctataa tttatcagta ataccggtaa
    16921 attatattca ttttttatac ataaatatac attctaaatt atattagatg tactaataca
    16981 attcaattaa ttttgtcttc tgaatttaaa ttaattatga agtgaaaaag tatcatgttt
    17041 agataaatat ttatgtgttt tttggatttt gatatattac ttttgtttta ttgatttata
    17101 acactaaaat aattaaacgt catgaaaaaa aacaattact tcttgttagc agtatttact
    17161 gccgttgtag ccttcacaag ccaacaagcg aaggctttcc ctccttacat ttacgaaggc
    17221 tttgattatg agattggtgc aacgccgctt aactacaatc ctttagttcc gggtacttgg
    17281 ggtgcggtac atatgtggaa acctgcatct agctggaatg gctatggagg ctcattttca
    17341 aacacttttc cctggggggt tactgatgtt tcacccatgg tcgttcctag tttggttcaa
    17401 acgggaaagt attttaacgg caatggtgag tgggtatcat gtttccttcg tgaaataaac
    17461 accgactgga attccccata ttgggattat atggatgaat caaaattagg aaagagtggc
    17521 actacacttt ggtttagtgt aatacttcgt ccgcagaaga ataagaaaga agcagcgctt
    17581 ggattatgca aagcatataa cgaacagagt gtaatgaaaa ttggtgcatt tggtggaaat
    17641 tactggggca tacgtgtaaa taacgatgcc gaacaagcac tttcgacagt tcctattgta
    17701 aacgacgagg ccaaatttct agtgtgcaaa gttgaataca cagatgttac cggtggaaca
    17761 atcactttat acattgatcc tactccaggt gtggagccat caaatgcagc agcaacacta
    17821 acaacgactt ccgatattag attcaaatct gttcagcttc ttacgcatta ctccgggggt
    17881 tgtatggctg ccgacgaaat tcgtattgcc accacctatg atgaggtagc ccccagtaca
    17941 ggaccatcaa ctggtttagt gcaagatatg aagaaagata ttcgtgttta tagccgggat
    18001 ggcaaggttg tggcagattt aaccatgctg aaaggcttgt caactgtttc ggttatcgac
    18061 accaaaggtg ccgttgtgaa aaccatc
//
LOCUS       DAOSCH010000034        17974 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2164_length_17974_cov_5.533735, whole genome shotgun sequence.
ACCESSION   DAOSCH010000034 DAOSCH010000000
VERSION     DAOSCH010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17974
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2164_length_17974_cov_5.533735"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1200)
                     /locus_tag="PLZ54_04180"
     CDS             complement(133..1200)
                     /locus_tag="PLZ54_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOJ66162.1"
                     /translation="MLLFFERIILGNRLSDWGISLLIIVGAFLLNKGISLLNKNVIQK
                     ITAKSKNRLDDLLVKTLEAPILLGIALIAVWVAARRLDLGTQVDQGFYRAYQILTVLN
                     ITWFLVKLTGALINEYLLPHSKEEEDNKRLDESMVRILRRISVGIIWAIGIVMALNNA
                     GVNVGALLASLGIGGLAVALAAQDTLKNFLGGLTIFMDRPFRIGDRIVTGAYDGFVED
                     VGIRSTRIRNFDRRIISIPNYKIVDAAIENISIEPMRRVVLKIGLTYNTPPEKMKEAI
                     AILKNIPQKVDEVFPDEIYAYFTDFSDSALTITFIYFIKKEANVMETPSKVNMNILED
                     FNAAGLNFAFPTRTVYIENQN"
     gene            complement(1242..3143)
                     /locus_tag="PLZ54_04185"
     CDS             complement(1242..3143)
                     /locus_tag="PLZ54_04185"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HOJ66163.1"
                     /translation="MNYGFVRIAAAVPELKVSDVLFNTEKIIELIHVAEENRTQVVCF
                     PELCITGYTCADLFFQQQLQEEVLKALSEIQMATLSVSCAVIVGAPLRFRNRLFNTAV
                     VLQKGRIIGIVPKTFLPNHGEFYEKRWFVSGKNLRGQTIVCNEQEVPFGVDLIFFSPS
                     FSFGIEICEDLWAPLPPSTELCMAGAEIIFNPSASNELIGKHEYRLQLIEQQSARCHA
                     GYVYSSSGMYESTTDLVFSGSAVIAENGKILETSKRFSLDSQLIFNEIDVEILRYERM
                     HNSNFGEDALQQTYRKIEMQGQPLEQKEIKRTVSPYPFVPPVSKREESCSEIFSIQTS
                     GLAKRWLHTQAKSLIVGISGGLDSTLALLVCVKTADKLGFDRKTVIGVTMPGFGTTNR
                     TYGNALNLMKSLEITGLEISIKEACLQHFKDIGHDPDVHDVIFENTQARERTQILMDL
                     ANKYNGLVIGTGDLSELALGWATYNGDHMSMYAVNSGIPKTLVRYLVEYVAQSMEEKT
                     KTILEDILKTPVSPELLLADEYGEIHQKTEDLVGPYELHDFFLYYFVRFGFSPKKILF
                     LAQKAFGDKYSEAILRKWLKVFLRRFYSQQFKRSCMPDGPKVGSINLSPRGDWRMPSD
                     AGFHWDDII"
     gene            complement(3154..3579)
                     /locus_tag="PLZ54_04190"
     CDS             complement(3154..3579)
                     /locus_tag="PLZ54_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOJ66164.1"
                     /translation="MKELTLITPTFLFSAVSLILLAYTNRFLSYAQLVRTLKDRYMED
                     HSAVTRAQILNLRKRLSLTRLMQLFGVASLLLCVATMFLIYVGFQTVSAYVFGIALVF
                     LIVSLSFSVWEIQISTGSLKIYLEEMENEEIREEEKLKT"
     gene            complement(3616..4497)
                     /locus_tag="PLZ54_04195"
     CDS             complement(3616..4497)
                     /locus_tag="PLZ54_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOJ66165.1"
                     /translation="MGKNNNLHGKRSIKNIHFISTFSMSLVLFLVGLVILLLLTARNL
                     GINVRENINLSIILDDEIKNTEIQRIQKYLLSSPYAKSVRFISKEEALQEHIESLGED
                     PQKFLGYNPLLASFEVKLKAEYANNDSVEMIESKLKRFDHINRIAYQKDVVDLVNRNI
                     QKLSYILLGIALVLLLVSIALINNTIRLLIYSNRFLINTMKLVGATWWFIRKPYIKQG
                     MINGLVASILSLLYLTATIYYVKYEFGISGIVIPFGTALMVSSIILFLGVTLTAISSY
                     LAVGRYLRMGTNNMYFV"
     gene            complement(4560..7058)
                     /locus_tag="PLZ54_04200"
     CDS             complement(4560..7058)
                     /locus_tag="PLZ54_04200"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOJ66166.1"
                     /translation="MNKSLFQKLLPHFVALILFFCLSFWYFSPVLEGKQLMGHDTQSW
                     LYSAKETIDYNQTHDDKTLWTNSMFGGMPTYQISMTQLNNIIKYVDQLIKSLPSPVFF
                     LVLYLLCFYILLLAFGINPWLAIIGSFAFSFASYNFIILSVGHNSKAITIAYIAPLIG
                     SIYMAFRSKRMLGSLLTALFLSLAIYANHIQILYYTLLMVLVFGLVELIFSIKEKKAS
                     SYFKTLGLLIIAALLAAGMNATSLLTTYEYNQYTMRGKSTGLTLDEQNAQHGLNKDYI
                     TQWSYGIDETLTLLIPNFKGGASAGTLSSESKTAQRLSSYGVPNVERMMKEMQLPLYW
                     GNQPGTAGPVYVGAIVCFLFVLGIFLVDKRTKWWLLPLIVLSIMLSWGKNFMPLTDFF
                     IRYIPFYNNFRTVSMILVIASFGMALMAILALKEVFNPATDKAKLLRPVSISAAIVGG
                     ICLIFAVVPSLAGNFVSPADGQFTGDYAFLKETLPLDREALLRSDALRSLTFVILCAG
                     LIWLYAKNLVKKNLAYVLLGCLMLGDLVPVAKRYLNDDNFTRKRSFEKFIQPSVADKI
                     ILQDKSQFRVLDATVDIFNDASPSYFHKNIGGYHAAKLRRYQELINIHLTKELGQLFG
                     AFGRAKTIEEIVQVMDSLGVLNMLNMKYVIYNKEAPPLVNPYANGNAWLVTKVRIAEN
                     ADEEMRLLGEINTKTEVVVDKSFSALLPPAIIPDSSAHIQLVSYEPNHLIYNFSSKTD
                     QLAIFSEIYYDKGWNAYINGKKVPYIRANYLLRAMPLKAGSYQIEFKFEPDSYRIGNS
                     IALVSSILFVLWLVGYIYLRWKKPSTAKKIDQWE"
     gene            complement(7203..8210)
                     /locus_tag="PLZ54_04205"
     CDS             complement(7203..8210)
                     /locus_tag="PLZ54_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ66167.1"
                     /translation="MKVLFIGGTGIISSACTEIAIEKGMEVFHLNRGMTDKDHVPKEV
                     TTIHADIRNGNEAEAKLKGHYFNSVVDFIAFEPQHIEQDIRLFRDRTAQYVFISTASA
                     YQKPPDKLPITEKTPLWNPYWEYSQKKIFCEKLLKSEAKKSGFPFTIVRPSHTYNYKK
                     VPAIGGYTVLHRMLKGQPVVLHGDGTSVWTLTHHRDFAVGLIGLLGNRAAIHKDFHIT
                     SDEWLTWNGIYKILANELGVEPKTVHVPSQIIALYDKEMGANLLGDKSHSMIFDNSKI
                     KKIVPEFRAEIPFKTGAAEVVQWFRENMLYKGPDAGVENWMNRLIEDWEGFKSFRGNV
                     K"
     gene            8481..10076
                     /gene="pckA"
                     /locus_tag="PLZ54_04210"
     CDS             8481..10076
                     /gene="pckA"
                     /locus_tag="PLZ54_04210"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOJ66168.1"
                     /translation="MANLDLSKYGITGEYEIVHNPTYEQLFRAETDPKNEGFERGVVT
                     TTGAVAVDTGIFTGRSPKDKYFVKDEITEKAMWWDGVINRPINTEVWDYCRGLVTDRL
                     GSVKKLYVVDCFCGTNPDTRMKVRFVMEVAWQAHFVTNMFIRPSHYELANFGEPDFVV
                     LNGSKVTNPKWKEQGLNSEVFVLFNLSQRMAVIGGTWYGGEMKKGIFSLMNFYLPQKG
                     IASMHCSANVGEDGDVAIFFGLSGTGKTTLSADPKRYLIGDDEHGWDDHGVFNYEGGC
                     YAKVIDLSEENEPDIWRAIRRDALLENVTVLPDGTPDFSKAASKTENTRVSYPIYYIN
                     KIVLPSKAGHASKIIYLSADAYGVLPPVSILDEKSAQYHFLCGYTSKLAGTERGINEP
                     VPSFSPAFGEAFLTLHPTIYAKVLAEKMKKHNAKAYLVNTGWNGTGKRISIKDTRAII
                     DAIIDGSIEKAEKVHIPIMNLTIPKSLNNVNPAILDPRDTYANPAEWEQKAKSLAAQY
                     IKNFEQYLDNENAKELLSAGPQL"
     gene            complement(10164..11159)
                     /gene="trpS"
                     /locus_tag="PLZ54_04215"
     CDS             complement(10164..11159)
                     /gene="trpS"
                     /locus_tag="PLZ54_04215"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOJ66169.1"
                     /translation="METVLSGIRPTGNLHLGNYFGALKNFVQMQNEYNCYFFIADYHS
                     LTTHPTPQNLTENVREILAEYLAAGLDPEKAVIYIQSDVPEVTELYLFLNMNAYVGEL
                     ERCTSFKDKVRQQPHNVNAGLLTYPTLMAADILLHRANKVPVGKDQEQHLEMTRTFAN
                     RFNRMYQVDYFPEPQAFNFGNELIKIPGLNGTGKMGKSEGEGNAIFLSDEPEAIRKKV
                     MKAVTDAGPTQPHQEKSQPIENLFQLMRAVSSAETLAYFEEQYDSCQIKYGEMKKQLA
                     EDMITFTAPFREKIKDLLSNKDYLSNVARMGKEKAQENARKTIRDVREIIGFKSI"
     gene            11364..12179
                     /locus_tag="PLZ54_04220"
     CDS             11364..12179
                     /locus_tag="PLZ54_04220"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOJ66170.1"
                     /translation="MEKKVILITGASSGIGLSTAIQLMNKGYRVYAASRSGSENSTSK
                     NGGEIISTKMNVNSEEEIKKVLDEILEQNGHLDAVISNAGNGIAGSIEDTSIEEAKYQ
                     FETNFFGAVRVIQACLPIFRKQGYGKIIATSSVAAIVPIPFQAFYSASKAALLIFMQA
                     LDMETKPFGIQCSTILPGDTKTNFTSARKYTAASQSTESVYYEKMKKAVGKMEKDEQN
                     GMPADAIAKAIVKQIVHKSMKAIVVPGFQYQVICWLFNRLPVKFRLKVVSMLY"
     gene            complement(12265..>12624)
                     /locus_tag="PLZ54_04225"
     CDS             complement(12265..>12624)
                     /locus_tag="PLZ54_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family zinc-binding domain-containing
                     protein"
                     /protein_id="HOJ66171.1"
                     /translation="AYEDRRERFVSRVQSVLNYAQEENVCRSQMLLSYFGEKNSQPCG
                     KCDVCQKKKETKISTAEFEAIRSSIVQVLSNENLPLDMAVHKIHFKEKKVLQVIRFML
                     DNNQIKENEKKQLILAK"
     assembly_gap    12625..12724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12725..14016)
                     /locus_tag="PLZ54_04230"
     CDS             complement(<12725..14016)
                     /locus_tag="PLZ54_04230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOJ66172.1"
                     /translation="MQKYLDILKQYWGYSSFRPLQDEIIGSIASGKDTLGLMPTGGGK
                     SLTFQVPAMTMEGVCVVVTPLIALMKDQVENLKKRGIAAAAIFSGMSYDEILMTLENA
                     SFGAYKFLYVSPERLATRIFIEKIKQTKVCLIAVDESHCISQWGYDFRPSYLKIAEIR
                     AFLPNAPVLALTATATPEVAEDIQKQLHFPASNIFKKSFYRSNLAYVVRYTEDKNQYL
                     LKILNSVPGTSVVYVRNRKKTKETAEFLVKNGIVAEHYHAGLNNVKKDEIQTRWKNGK
                     TRVIVSTNAFGMGIDKADVRSVIHLDLPDSLEAYFQEAGRAGRDEKKAFAVLLFNNSD
                     AAKIKKRISDTFPPKEMIRKVYESLGNYYQVGIGSGLDNTFPFDLVDFCTKFHLPVLM
                     AFSSLKILQQAGYIEFTDEEESTSQVLFTVKKEELYQL"
     gene            14293..16731
                     /gene="topA"
                     /locus_tag="PLZ54_04235"
     CDS             14293..16731
                     /gene="topA"
                     /locus_tag="PLZ54_04235"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008662589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOJ66173.1"
                     /translation="MPKNLVIVESPAKAKTIEKFLGKEYHVVSSYGHIRDLSEKGIGI
                     DFEHNYQPQYKVLPDKKKLVSELKKATEQAELVWLASDEDREGEAIAWHLSEELKLPK
                     EKTRRIVFHEITKDAIVHAIEHPRDLDLNLVDAQQARRVLDRIVGFELSPLLWKKIRP
                     SLSAGRVQSVAVRLIVEREREINQFVPESSYRVTAIFSTTDKDGRSVELKAELQQRFS
                     TKEEALNFLNQCKQAIFRVENITTKPTKRSPAPPFTTSTLQQEAARKLGFSVSQTMLI
                     AQHLYESGKITYMRTDSVNLSDFALKAAEAEITNMLGKKYWKLRHYVTKTKGAQEAHE
                     AIRPTYMNVHEIDGSDQEKRLYDLIWKRTLASQMADAEFEKTTIQIGMQGQPYQFVAS
                     GEVIIFDGFLHLYSESTDDESDSEKSEILPAINVGEKLLPKEILAQERFTQRPPRYTE
                     ASLVRKLEELGIGRPSTYAPTISTIQNRKYIEKGNRPGEKRTYNILKLVDGKISDQSA
                     TEITGAEKAKLFPTDIGIVVNDFLIEYFPGILSYNFTANVEKEFDNVAAGKVGWTQVI
                     DKFYKKFHPTVEKTLTSTERKVGGRTLGTDPKTGKPVTVKIGRYGPYVQIGTAEDAEN
                     PVFASLRPGQSIDSITLEEALDLFKLPREVGEFEGKPLTVAIGRFGPYIRQGNSFYSI
                     PKGEDPLNVSLERAIEIIQHKREEDQKKTIKTLGENKEIQILNGRFGPYISFEKKNYK
                     IPKNVDPTGLTFEQCMEMIQEQAGTDKKGKKGNTRKTASTEKSAKNTKTSKTTKTTKT
                     SKTKKSTTAGKKKV"
     gene            17075..>17974
                     /locus_tag="PLZ54_04240"
     CDS             17075..>17974
                     /locus_tag="PLZ54_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOJ66174.1"
                     /translation="MNNLKTNIMKFNKKIKWLLIRLLLLFSGFLGAQTRTITGIVSST
                     EDGLPLIGVTVMEKGTRNGTVTNNDGQYNISVSSPNSILQFSMVGMENVEEKVGNRKI
                     IDVRMSPSTVLLDQIVVTGYTTEKKADLTGAVSVVDVKDMMSESGNNPILALQGRVAG
                     MSITADGNPSGAATVRIRGIGTMNNNDPLYVIDGVPTKGGMHELNSNDIESIQVLRDA
                     SAATIYGSRAANGVIIITTKQGEKGKTNVNFDSYLTLSQYGKTVEMMNTKQYGESLWQ
                     AMINSGYDPNANGIGYMYNYSYDD"
BASE COUNT         5601 a         3540 c         3503 g         5230 t
ORIGIN      
        1 cgagcttgcc gagcgattgt ttttctaaag atgcttctgt gccgtttccg tttttttcat
       61 gttcttattt gtatcttaaa cgagttcacc attaagctac cacaaaatta ccggtctttt
      121 ggattccgat tgtcagtttt gattttcaat gtacacagta cgcgtaggga aagcaaagtt
      181 tagacctgct gcattgaaat cttccaaaat attcatattt acctttgagg gtgtttccat
      241 cacatttgcc tcttttttaa taaagtaaat aaatgttatt gtcagtgccg aatcggaaaa
      301 gtcggtgaaa taagcataaa tttcatctgg aaacacctca tcaacttttt gcggaatatt
      361 tttcaaaata gcaatggctt ctttcatctt ttcaggagga gtgttgtaag taaggccgat
      421 tttcagcact acgcgccgca tgggttcaat ggaaatattt tctatggcgg catctacaat
      481 tttgtaattg ggaatggata tgattcttct gtcgaaattt cgtattctgg tgctgcgaat
      541 gccaacatct tcaacaaaac cgtcataggc tccggtaaca atccgatcgc ctatgcgaaa
      601 gggccgatcc ataaaaatgg tgagtccgcc aagaaaattt tttaacgtgt cctgtgccgc
      661 caaagccact gccaaacctc caattcccaa actggccagc agagctccca cattgacacc
      721 tgcattgttc aatgccatca caatgccaat agcccaaata atgcccaccg aaatgcgtcg
      781 taaaattctg accatgcttt catcgagcct tttgttatct tcctcttcct ttgagtgcgg
      841 cagaagatat tcattgatca gggcaccggt taattttacc aaaaaccatg tgatattaag
      901 aaccgtcaat atctgataag cgcgataaaa gccttggtcg acctgtgtac ccaaatcgag
      961 acggcgagca gccacccata cggcaatcaa agcaatacca agtagaatag gcgcttcgag
     1021 tgtttttacc aataaatcgt ccagccgatt tttacttttg gccgtaattt tctgaattac
     1081 gtttttgttc aaaagtgaaa tacctttatt caacaaaaat gcacccacaa ttatcaacag
     1141 agagattccc caatcggaca gtcggtttcc caaaattatt ctttcaaaaa ataataacat
     1201 gaattttaaa tttatgaaat gaaacagtgt ttataaagaa atcaaattat atcgtcccaa
     1261 tgaaaaccgg catcgctcgg catccgccag tcgccacgcg gcgaaagatt gatggagccg
     1321 acttttgggc catcgggcat acacgaacgc ttgaattgtt gcgaataaaa acgccgtaaa
     1381 aaaactttta gccatttccg caggattgct tccgaatatt tatcgccaaa tgctttttgc
     1441 gccaaaaaaa gaattttttt tggactgaag ccaaaacgaa caaaatagta aagaaagaaa
     1501 tcgtgcaact cgtaaggacc caccaaatct tccgtctttt gatgaatttc tccatactca
     1561 tcagctagta ataattcagg actaacaggc gttttcaaaa tatcttccaa aattgttttg
     1621 gttttttctt ccatactttg agccacatat tctaccagat aacgaaccaa agttttggga
     1681 ataccgctgt taacggcata catcgacatg tgatcgccgt tgtatgttgc ccatcccagc
     1741 gccaattccg aaaggtcgcc ggttccaatc accaatccgt tgtatttatt tgccaaatcc
     1801 attaaaattt gcgttcgttc acgtgcctgt gtattttcaa aaatcacgtc gtgaacatcc
     1861 gggtcgtgtc caatgtcttt gaaatgttgc agacaagctt ccttgatgga aatttccaaa
     1921 ccggtaattt ccaaagattt catcaaattc aacgcattgc catacgttcg gttggttgtg
     1981 ccaaaaccgg gcatggtaac cccaataaca gtttttctgt caaaacccaa tttgtcggca
     2041 gtttttacac acaccagcaa agcaagcgta gaatccagcc ctccggaaat tccaactatc
     2101 agcgatttgg cttgtgtatg cagccagcgt ttggccaacc ccgacgtttg aatggaaaaa
     2161 atctcgctgc agctttcttc tcttttcgat acaggtggaa caaacgggta gggtgaaacc
     2221 gttcttttaa tttccttctg ttcgagaggt tgcccctgca tttcaatttt cctgtaagtc
     2281 tgctgtaaag catcttcacc gaaattactg ttgtgcattc tttcgtaccg caatatttcg
     2341 acatctattt cgttgaaaat cagctgagaa tcgagcgaaa atcttttgga agtttcgaga
     2401 atttttccgt tttctgcaat gactgcactt cctgaaaaga ccaagtcggt agtcgattcg
     2461 tacattccgg aagaagaata aacatatccg gcatggcaac gagccgactg ttgttcaata
     2521 agttgcagtc gatattcgtg ttttccaatc aattcattgc tggccgaagg attaaaaata
     2581 atttctgccc ctgccatgca caattcggta ctgggaggaa gcggtgccca taaatcttca
     2641 caaatttcga ttccaaaaga aaatgaagga gaaaaaaata ttaaatcgac gccaaaaggc
     2701 acttcctgtt cattgcacac aatagtttgt ccacgaagat tttttccgga aacaaaccat
     2761 cgcttttcgt aaaattcgcc atgattgggc aaaaaagttt tagggacgat gccgataatc
     2821 cgcccttttt gtaagacgac cgccgtattg aaaagccgat tgcgaaaacg aagcggtgct
     2881 cccacgataa cagcacagga aacggacaag gtggccatct gaatttcaga cagggctttc
     2941 aatacttctt cttgcaattg ttgctgaaaa aataaatcgg cgcacgtgta accggtgata
     3001 cacaattcgg ggaaacagac cacttgggtc cggttttctt cggcaacatg aatcagttct
     3061 attatttttt cagtgttaaa caaaacatcc gaaactttga gttcgggaac cgcagctgct
     3121 atgcggacaa aaccataatt tatttccatt tcttcaagtt ttaagttttt cttcctccct
     3181 gatttcttcg ttttccattt cctccaaata aatcttgagc gaaccggtag aaatctgaat
     3241 ttcccaaaca gaaaaggaaa gagaaacaat caggaataca agtgcaatac cgaaaacata
     3301 ggcggaaacg gtttgaaaac cgacgtaaat aagaaacatg gtagctacac agagcaataa
     3361 actagccacg ccaaacaatt gcatcagtcg cgtaagcgaa agtcttttac ggagatttaa
     3421 aatctgagcc cgcgtaactg ccgaatgatc ttccatgtaa cgatctttaa gtgttcgcac
     3481 caactgggca tacgacagaa aacgattggt ataagccagc aaaataagcg aaactgccga
     3541 aaaaagaaaa gtcggtgtaa ttaaggtgag ttctttcatg gtaaagacag ttacaaatta
     3601 aaaaaccgaa aaaaattata caaaatacat attgttggtg cccatacgca aatatctgcc
     3661 taccgccaaa taggatgaaa tagcagtaag cgtaacaccc aaaaacaaaa taatagagga
     3721 taccatcaaa gctgtaccaa agggaattac tattcccgaa ataccaaatt catacttaac
     3781 ataataaatc gtagcagtca aataaagcag agaaaggata gaagccacaa gtccattgat
     3841 cattccctgc ttaatataag gtttgcggat aaaccaccag gtagcaccca ccaatttcat
     3901 ggtgttgata agaaaacgat tggaataaat cagcagtcga atggtgttgt ttatcaatgc
     3961 tatagaaaca agcaacaata ccaaagcaat tccgagcaga atgtaactca atttctgaat
     4021 attccgattt accaaatcta ctacatcttt ctgataggct atgcgattga tatggtcaaa
     4081 tctttttagt ttagattcaa tcatttcaac actgtcgtta ttggcatatt cggctttcag
     4141 tttcacctcg aaagaagcca gcaaagggtt gtatcccaaa aacttttgtg gatcttcgcc
     4201 caaactttcg atatgctcct gcaaagcttc ctctttggag ataaacctca ccgatttggc
     4261 ataaggagaa gaaagaagat atttttgaat tctctgaatc tctgtgtttt taatttcatc
     4321 gtccaaaata atggaaagat taatgttctc tcttacgttt atgcccagat ttcttgccgt
     4381 caaaagcaat aaaatcacca gtccaaccaa aaacagcacc aacgacatgc tgaaggtaga
     4441 aataaaatgt atgtttttta tacttcgttt accgtgaaga ttattgtttt tacccatttc
     4501 cgatattctt tttcaaataa ttatctcttt gaacccttta caaatcctta ttccagaaat
     4561 tattcccatt ggtcgatttt ctttgccgta gaaggttttt tccatcttaa ataaatataa
     4621 cccaccaacc aaagcacaaa caatatggaa gaaacaaggg ctatactgtt cccaattctg
     4681 taagaatcgg gttcaaattt gaattctatt tgataagaac ccgctttcag tggcattgct
     4741 ctaagcaagt agttggcacg aatataggga actttcttgc cgttgatata ggcattccat
     4801 cccttatcgt aatatatttc cgaaaaaata gcgagctgat cggttttgga actgaaattg
     4861 taaatcaggt gattgggttc gtagcttacc agttgaatgt gagccgacga atcgggaata
     4921 atggcaggag gcaatagcgc agaaaaagat ttgtccacca ccacttctgt tttggtattg
     4981 atttcaccca acaaacgcat ttcttcatct gcattttcgg ctattctaac ttttgtcacc
     5041 aaccatgcat tgccattggc ataaggattg accaatggag gcgcttcctt gttgtaaatt
     5101 acatatttca tgttcagcat atttaaaacg cccaaagaat ccattacctg cacaatctct
     5161 tcaattgttt tggctcttcc aaacgctcca aacaattgtc cgagctcctt agtgagatga
     5221 atgttgataa gttcctgata acgacgcaat tttgccgcat ggtatccgcc tatatttttg
     5281 tgaaaatatg aaggtgaagc atcgttgaaa atatccactg tggcatcgag taccctgaat
     5341 tgcgacttgt cctgaagaat aattttatcg gctaccgatg gctgaataaa tttttcaaag
     5401 cttctttttc gcgtaaaatt atcatcgttc aaatatcgtt tggcaaccgg caccaggtct
     5461 cccagcatca agcatcccaa caaaacatag gcaagattct ttttaaccag atttttggca
     5521 tacagccaaa tcaagccggc acaaagaata acaaaagtca atgaacgcaa agcatcgctg
     5581 cggagtaaag cttctctgtc gaggggaagt gtttctttca aaaaagcata atcacccgta
     5641 aattgcccat cggcaggcga aacaaaattc ccggccaaag aaggaacaac agcaaaaatc
     5701 agacaaatac caccaactat tgcggcgctg atggaaacag gacgcaaaag ttttgccttg
     5761 tctgttgcgg gattgaatac ttcctttaag gcgagtattg ccattaatgc cataccaaaa
     5821 ctggcaatga ccaaaatcat ggaaaccgtg cggaaattat tgtaaaaggg aatatatcgg
     5881 atgaaaaagt cggtaagggg catgaaattt ttcccccacg aaagcataat agaaagaaca
     5941 attaatggca acaaccacca ttttgtacgc ttgtccacca aaaaaattcc caacacaaac
     6001 agaaaacaga cgatggctcc aacatacacc ggtccggcag ttcccggctg attaccccaa
     6061 tacagaggta attgcatctc cttcatcatt ctttccacat tgggaacccc ataacttgac
     6121 agtcgctggg ctgttttgct ttcggatgaa agtgtgccgg cactggcacc tcctttaaaa
     6181 ttcgggataa gcaatgtcag cgtttcatca attccgtagc tccattgtgt aatataatcc
     6241 ttgttcagtc cgtgttgcgc attttgttca tcaagggtaa gtcctgtcga ttttccgcgc
     6301 atggtgtact gattgtattc gtaggtagta agtaaactcg tggcattcat tcctgctgcc
     6361 agcaaagcag caataatcaa caatcccaac gttttaaaat aagaacttgc cttcttttcc
     6421 tttatactga agatcagttc caccaatcca aaaaccaaaa ccataagcaa agtataataa
     6481 agaatttgga tatggttggc ataaatggct aaactcagga aaagtgccgt caataggctt
     6541 cccaacattc tcttggaacg gaaagccatg taaatgctcc cgataagcgg tgcaatgtaa
     6601 gctatcgtaa tggctttgga attatgtccg acagacagaa taataaaatt gtaagaggca
     6661 aaggaaaagg caaatgaacc aataatggca agccagggat taataccaaa agctaaaagc
     6721 aaaatataaa aacacaaaag ataaagaacc agaaaaaata ccggtgaagg caaggatttg
     6781 ataagttggt cgacatattt aataatattg ttaagttgcg tcattgaaat ttgataagtc
     6841 ggcatcccac caaacatgga gttagtccat aatgttttat catcatgtgt ctggttataa
     6901 tcaatggtct ctttggctga gtacaaccag ctttgggtat cgtgtcccat cagttgtttg
     6961 ccttccaaaa cgggagaaaa ataccaaaac gacaaacaaa aaaacaaaat aagtgctaca
     7021 aaatgaggca gcaatttttg aaataaactt ttgttcataa gaatgatggt ttatattttc
     7081 agctaaatga ggaaatttga gtacaaaaat acattatttt ttttcaaaag ctctttctca
     7141 taccttaaaa ttacagtaac ttgcatttag gaataaaagc cttatggttt acgaaacttt
     7201 aatcatttaa cgttgcctcg aaacgacttg aatccttccc aatcttctat tagtcgattc
     7261 atccaatttt ccacaccggc atctggtcct ttatacagca tgttttctct gaaccattga
     7321 acaacctctg ctgcacctgt tttaaatgga atttcggcac gaaattcggg cacaattttt
     7381 tttattttgg aattgtcgaa aatcatgctg tgcgatttgt ctcccaataa attggcgccc
     7441 atctccttat cgtacaaggc aatgatttga gatggaacat gtaccgtctt tggctcaaca
     7501 cccaattcat ttgccaaaat tttataaatt ccgttccacg tcagccattc gtcggaagtg
     7561 atatgaaaat ctttgtgaat ggctgctctg tttcccaaca atccgattaa tccgactgca
     7621 aaatcgcgat gatgggtcaa agtccaaact gaagttccat caccatgtaa cactaccggt
     7681 tgacccttca acatccgatg taatacagtg taacccccta tggccggcac ttttttatag
     7741 ttgtaggtat gcgaaggacg aactatggta aaaggaaagc cggatttttt tgcttcactt
     7801 ttcaatagtt tttcacagaa aattttcttt tgggagtatt cccaataagg attccatagt
     7861 ggagtttttt cggtaattgg caatttatcg ggtggcttct gataagccga tgcagtactg
     7921 ataaacacat attgagcggt acggtctcga aacagtcgga tatcctgctc gatatgttgc
     7981 ggctcaaaag cgatgaaatc gacaacggaa ttaaaataat gtcctttcag cttagcctct
     8041 gcttcattgc cgttgcgaat atcagcatga atggttgtaa cttctttcgg cacatggtcc
     8101 ttatcggtca ttccacgatt caaatgaaat acttccatcc ctttctcaat ggctatttcc
     8161 gtacatgccg aactgataat gcctgttccg ccaataaata aaactttcat cagaatataa
     8221 tttatttttt aatggtctga tggaacccac atgtctgaat attatattca ttcgtgtttg
     8281 tgtatgtaat acatgtgggt ttcataaact ttaaagtttt tcgtttaact caacgaagtt
     8341 acagtttttt ttgtaattaa ttaaagaaat ttttctttaa ttcctttgtc gtgaaaaaaa
     8401 tagttgtaat tttgcgacgt gaaaaacaag gcttttcaga aaaaagataa aaacaaacat
     8461 aacaattaat ttaaaaaaaa atggcaaatt tagatttaag taagtacggt attaccggcg
     8521 agtacgaaat cgtacacaat cctacttatg aacaactgtt cagagcagaa accgacccta
     8581 aaaacgaagg ttttgaacgc ggtgttgtta ccacaaccgg tgcagtagca gtagataccg
     8641 gtatttttac cggacgttcg cccaaagata aatattttgt aaaagatgaa attaccgaaa
     8701 aagccatgtg gtgggacggg gttattaatc gacccatcaa cacggaggtt tgggactatt
     8761 gcagaggctt ggtaaccgac aggctgggtt ctgtgaaaaa actttatgtg gtagattgtt
     8821 tctgcggtac caatcccgat acgcgaatga aagtgcgctt tgttatggaa gtggcttggc
     8881 aggcacattt tgtaaccaac atgtttatcc gtccttcaca ctatgaactg gcaaattttg
     8941 gagaacccga ttttgttgtg ctcaatggtt ctaaggttac caatcctaaa tggaaggaac
     9001 agggattgaa ctctgaagtt tttgttcttt tcaatctttc tcagcgtatg gccgttatcg
     9061 gtggaacatg gtacggcggt gaaatgaaga aaggaatttt ctcactgatg aatttttatc
     9121 tgccacagaa aggtatagcc tccatgcact gctctgccaa tgttggcgaa gacggagatg
     9181 tagccatttt ctttggtttg tcggggacag gaaaaacaac tctttctgcc gatccaaaac
     9241 gctatttaat tggtgacgac gaacacggat gggatgatca cggcgtattc aattacgagg
     9301 gtggatgtta tgctaaagtt atcgacttaa gcgaagaaaa cgaaccggat atctggcgtg
     9361 ctattcgtcg ggatgctttg ttggaaaatg ttaccgtttt gcccgatggc acacccgact
     9421 tttcaaaagc agcttctaaa acggaaaata cacgtgtttc ttacccaatt tattatatca
     9481 ataaaattgt gcttccttca aaagccggac acgcaagcaa aattatatat ttgtcggcag
     9541 atgcttacgg cgttttgcct ccggtttcta ttttggatga aaaatcggca cagtatcact
     9601 tcctttgcgg atatacttca aaattggcag gaaccgaacg cggtatcaac gaacccgttc
     9661 cttccttctc gcctgcattt ggagaagcat ttttaacatt gcatcctacc atttatgcca
     9721 aagtgttggc cgaaaaaatg aaaaagcaca atgcaaaggc ttatttggtt aataccggtt
     9781 ggaatggtac tggcaaacgt atttccatta aggacacccg ggccattatc gatgccatta
     9841 ttgacggttc aatcgaaaaa gcagaaaaag ttcatattcc aattatgaat ctgactattc
     9901 caaaatcttt gaacaatgtg aaccctgcta ttcttgatcc gcgcgacacc tatgctaatc
     9961 cggcagaatg ggaacagaaa gcaaaatctt tggctgctca atatatcaag aactttgaac
    10021 agtatctcga taacgaaaat gccaaagaac ttttatctgc aggccctcaa ctttaataag
    10081 ttacatacct gaaatataaa aagggagccg ttcatttttg agcagttccc ttttttcttc
    10141 taaaaagtag agaaagaagt tatttatatt gatttgaaac cgattatttc gcgaacatcg
    10201 cgaatggttt tccgcgcatt ttcctgtgct ttttcttttc ccatgcgggc aacattcgac
    10261 aggtaatctt tattgctcag aaggtcttta attttttcac gaaacggagc agtaaaagta
    10321 atcatatctt ctgccagctg ttttttcatt tccccgtatt tgatttggca agaatcgtat
    10381 tgttcttcaa aataagccaa agtttcggca ctggatacgg ctctcatcaa ttgaaaaaga
    10441 ttttcgatgg gttgagattt ttcctggtga ggctgtgtgg ggcctgcatc ggttaccgct
    10501 ttcattactt ttttccgaat tgcctccggc tcgtccgata agaaaatggc attgccttct
    10561 ccttccgatt ttcccatttt tcccgtaccg ttgagtcccg gaattttaat cagttcgttt
    10621 ccgaaattaa aagcttgcgg ttcagggaaa taatctacct gatacattct gttgaatcga
    10681 ttggcaaaag tccgcgtcat ttcgagatgt tgttcctggt ctttgcctac cggaaccttg
    10741 ttggcgcgat gcagcaaaat atcggcagcc atcaatgtcg gataagtcaa cagtccggca
    10801 tttacgttgt ggggttgctg gcgaacttta tctttgaagg aagtacagcg ttccaattca
    10861 ccgacgtaag cattcatgtt caagaaaaga tacagttccg taacttcggg cacatcactc
    10921 tgtatgtaaa taactgcttt ttcgggatca aggcctgcag ccaaatattc cgctaaaatt
    10981 tctctcacat tttccgtcag attttgcggt gtgggatgcg tggtcagtga gtggtaatcg
    11041 gctataaaaa aataacaatt gtattcattt tgcatttgta caaaattctt caatgcgcca
    11101 aaatagtttc ccaaatgcaa gttgccggtc ggacgtatgc cgctaagtac agtttccatc
    11161 aaaaataaaa aattttagtg ttgtatttta aattcaaatg caaaattaat cattttagga
    11221 gaaatatcca gcttctcaat taaagtcaga ggaatttgat tcggagagaa gcaaaagaat
    11281 ttttccaaaa aacgttggaa atataaatct ttctttgcat ttgattattt ttgcagaaaa
    11341 atttttaaaa atggagatgt aatatggaga agaaagtaat attaataacc ggagcaagca
    11401 gcggtattgg attatcaaca gccattcaac taatgaacaa gggttacagg gtttatgctg
    11461 cctcccgtag tggtagtgaa aatagcacct ccaaaaatgg cggagaaatt atttctacaa
    11521 aaatgaatgt gaatagtgaa gaagaaataa aaaaagtgtt ggacgagatt ttggaacaaa
    11581 atgggcattt ggatgctgtt atttccaatg caggtaacgg catagccggt tctatcgaag
    11641 acacttccat tgaagaagcc aaatatcagt ttgaaacgaa tttttttgga gcagtaagag
    11701 ttattcaggc ctgtttgccc attttcagga aacagggcta tggaaaaatc attgccacat
    11761 cttcggtagc tgccattgtt cctattcctt ttcaggcatt ttattccgct tccaaagcag
    11821 ctttgcttat ttttatgcag gcgcttgaca tggaaaccaa accttttggc attcagtgca
    11881 gcaccatttt accgggtgat accaaaacca atttcacgtc ggcaagaaaa tataccgcag
    11941 cttctcaatc gaccgaatcg gtttattacg aaaaaatgaa aaaagctgtc ggaaaaatgg
    12001 aaaaagatga acaaaacggg atgcccgccg atgctattgc aaaagccatt gtcaagcaaa
    12061 ttgtacacaa aagcatgaaa gcaatcgtag ttccgggatt tcagtatcag gttatttgct
    12121 ggcttttcaa ccgattgccc gtcaaattca ggctgaaagt ggtaagtatg ctctattagt
    12181 tcctgaaagt tttgctgata cgctgaaagt acatttaaac gattaaaaaa cagctgaaaa
    12241 tagaaaacag tggagcgggt gattttattt cgccaaaata agctgttttt tttcgttttc
    12301 ctttatttga ttgttgtcga gcataaagcg gataacctgc aatacctttt tttctttaaa
    12361 atgaatttta tgaacagcca tatcgagcgg aagattttcg tttgagagta cctgaacaat
    12421 tgacgaacga atggcttcaa attcggcagt agaaatttta gtttctttct tcttttgaca
    12481 cacgtcgcat ttgccacaag gctgactgtt tttttcgccg aagtaggaaa gcaacatttg
    12541 acttcgacag acattttctt cttgggcata gttcagtaca ctctgcacgc gggaaacaaa
    12601 tcgctctcgt cggtcttcat aagcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnntttagt tgatacaatt cctctttttt aaccgtgaaa agcacttgtg atgtactttc
    12781 ctcttcatcg gtaaattcaa tataaccggc ttgctgaagg attttcaggc tgctgaaagc
    12841 cattaataca ggcaagtgaa atttagtgca aaaatcgact aaatcgaaag gaaaagtatt
    12901 gtccagtcct gagcctatac caacctgata ataattgccc aaagattcgt aaactttgcg
    12961 aatcatttct tttggcggaa aagtatccga aatgcgtttt ttaatttttg cagcatcgct
    13021 gttgttgaaa agcagcacgg caaaagcttt tttttcatct cggcctgctc ttccggcttc
    13081 ctgaaaatat gcttccagcg aatcgggcaa atccaaatga atcactgacc gtacatcggc
    13141 tttgtcgatt cccatgccaa aagcgttggt ggaaacaatg acgcgggttt tcccgttttt
    13201 ccagcgtgtt tgaatttcgt cctttttcac gttgttgagg cctgcatgat aatgttcggc
    13261 tacaattccg ttttttacaa gaaactcggc tgtttccttt gttttttttc tgtttcgcac
    13321 ataaactacc gaagtgcccg gtacactgtt gagaattttc aacaaatact gatttttatc
    13381 ttcggtatat cgaaccacat aagccaaatt ggaacgataa aaactttttt tgaaaatgtt
    13441 ggatgccgga aaatgtaatt gtttctgtat gtcttcggcg acttcaggag tggcagtagc
    13501 cgtaagtgca agtacgggtg cattgggtaa aaacgctctg atttcggcaa ttttcaggta
    13561 ggaaggtcga aagtcgtaac cccattgaga aatacagtgc gattcgtcca cagcaatgag
    13621 acaaactttt gtctgtttta ttttttcgat aaaaatgcgt gttgcaagtc tttcgggcga
    13681 tacataaaga aatttgtagg ctccaaatga agcattttcg agcgtcatca gaatttcgtc
    13741 gtaagacatg ccagagaaaa tggcagcagc cgcaattcct ctttttttca agttttctac
    13801 ctgatctttc atcagtgcaa tcaagggcgt aacaaccacg caaactcctt ccattgtcat
    13861 ggcaggcacc tgaaaagtca gtgattttcc tccgccggta ggcataagtc ccaacgtatc
    13921 ttttccggaa gctatgctac cgataatttc atcctgtaat ggtcgaaatg aagaatatcc
    13981 ccagtattgc ttgagaatgt caaggtattt ctgcataaat taattgcaac tttattacaa
    14041 aaataatgat ttttactgaa agttcggatt tttatatgta aatcaggaaa gagaaaaaaa
    14101 ggtgaaaatt gtccactaaa ttagattatt tccttttatt caatgaagtg aacattttgt
    14161 aactttcagc tcattttctg ttttcgtttt ataaatttta aaaataataa tctgaaaatt
    14221 tggctgatac ctttcaaatt ttgttacttt gcaggcaatt ttaaataccg cttattaatt
    14281 taaagttgta atatgccaaa aaaccttgtt attgtagaat ctccggccaa agccaaaact
    14341 atcgaaaaat ttctgggaaa agaatatcat gttgtttcaa gttacggaca tattcgtgat
    14401 ttatccgaaa aaggcattgg tattgatttt gaacacaatt atcagcctca atacaaagtt
    14461 ttacccgaca agaaaaaact ggtttcggaa ttaaagaaag ccactgaaca ggcagagttg
    14521 gtttggctgg catctgatga agaccgcgaa ggagaagcta ttgcatggca tttgtcggaa
    14581 gaacttaaac tgccaaaaga aaagacacgc agaattgtat ttcatgaaat aaccaaagat
    14641 gccattgttc acgccattga gcatccgcga gatttggatt tgaatttggt agatgcccag
    14701 caggcgcgtc gtgtcctcga ccgaattgtt ggttttgaac tttctccgct gttatggaaa
    14761 aaaattcgtc cttccctttc ggcagggcgt gttcagtcgg tagctgtccg tctgattgtg
    14821 gaacgcgaaa gagaaatcaa tcagtttgtt cccgaatcgt cttatcgcgt aacggcaatt
    14881 ttcagtacaa cagacaaaga tggtcgttcc gtagaattga aggcagaact tcaacaacgt
    14941 ttttccacca aagaagaagc attgaatttt ttaaatcaat gtaagcaagc aatttttcgg
    15001 gtggaaaaca ttactaccaa accgaccaaa agatcacccg caccgccttt taccacttct
    15061 actcttcagc aggaagctgc acggaaactc ggtttttcgg tttctcaaac catgttgata
    15121 gctcagcatc tttacgaatc gggaaaaata acctatatgc gaaccgattc agtgaatttg
    15181 tcggattttg ccctgaaggc ggcagaagcg gaaattacca acatgttggg gaaaaaatat
    15241 tggaaattgc gacattatgt tacaaaaacc aaaggcgctc aggaagctca tgaagcgatt
    15301 cgtcctactt atatgaatgt tcacgaaatt gatggttcag accaggaaaa gcgattgtat
    15361 gacctgatat ggaaacgtac gctggcttca caaatggcgg atgcggaatt tgaaaaaacc
    15421 accattcaaa ttggaatgca aggtcaacct taccagtttg tggcttcggg tgaagtgatt
    15481 atatttgatg gttttcttca tctttacagc gaatcgacag atgatgagtc cgattcagaa
    15541 aaatcggaaa ttttaccggc cattaatgtg ggcgaaaaat tgttgccaaa agaaattctc
    15601 gctcaggaac gttttaccca gcgtccgccc cgatatacgg aagcaagttt ggtgcgaaaa
    15661 ctcgaagaat tgggcattgg gcgaccttct acgtatgctc caactatttc gactattcaa
    15721 aatcgaaaat acatagaaaa aggaaaccgt ccgggcgaaa agcgaactta caatatactg
    15781 aagcttgttg acggaaaaat ttccgatcag tcggctactg aaattaccgg tgccgaaaaa
    15841 gcaaaattat ttccgactga tatcggtata gtggtcaacg attttttaat cgaatatttt
    15901 cccggtattc tgagttataa cttcacggcc aatgtggaaa aggaatttga taatgtggcg
    15961 gcgggaaaag taggttggac gcaagttatc gacaagtttt acaaaaaatt tcatccaacg
    16021 gttgaaaaaa ccttaacttc caccgagcga aaagttggcg gtcgcacgtt gggtaccgat
    16081 cccaaaacag ggaaaccggt tacggttaaa ataggaagat atgggcctta tgttcaaatt
    16141 ggaacggctg aagatgctga aaatcctgtt tttgcttcgc tgcgtccggg tcagtccatc
    16201 gacagcatta cactcgaaga agcgctcgat ttgtttaaac tcccgcgtga agttggagaa
    16261 tttgaaggaa aaccgctgac tgttgccatt ggacgttttg gtccttatat ccgtcaggga
    16321 aattcgtttt attccatacc taaaggggaa gatcctttga atgtttcact ggaacgcgcc
    16381 atagaaatta ttcaacacaa acgggaggaa gatcagaaga aaactatcaa aactttaggc
    16441 gaaaacaaag agattcaaat tttaaacgga cgcttcgggc cttacatttc ttttgaaaaa
    16501 aagaactaca aaattcctaa aaatgtcgat cctacaggtt taacttttga acaatgtatg
    16561 gaaatgattc aggaacaggc cggaacagat aaaaaaggga aaaagggaaa tacgaggaaa
    16621 acagcttcga ccgaaaagtc tgcaaaaaat acaaaaacct ccaaaacgac taaaacgacc
    16681 aaaacatcaa aaacaaaaaa atctacgact gctggcaaga aaaaagttta ataaaaaatt
    16741 caggaaacat ttctgggcgc aagtaggaaa tttggcagag gaagaaagac cggttttttc
    16801 ttcctctgct atttttttgt ctgtatgaga aaaaattcat ttttttggag aataatggaa
    16861 atattttttg caaaatacta ttagctgtcc ttttaaacac ttaatcagac caataaaatt
    16921 cttttgaaga aacattgaaa tttttatcaa tgaaacaaaa atacatcaca atgaatcaaa
    16981 atttatagtg aagagaattt gtttcatcta attttgttac cgaaagcgct gagaattttc
    17041 ctttgaagga tgagaaaaac aaattaaatt tttaatgaat aacttaaaaa ctaatattat
    17101 gaagttcaac aaaaaaatta aatggctatt gataaggtta cttctattgt tttctggttt
    17161 tttaggggct caaaccagaa caattacggg aattgtttca tctacagaag atggattgcc
    17221 attgattggc gtaactgtta tggaaaaagg cactaggaat ggaactgtta ccaacaatga
    17281 tggtcagtat aacatctcag tatcttctcc taattctata ctacaatttt caatggtagg
    17341 gatggaaaat gtagaggaaa aagttggaaa tagaaaaata attgatgtga gaatgtcccc
    17401 ttcaactgtt ttattagatc aaattgttgt tactggttat acaacagaaa aaaaagccga
    17461 tttaactggt gcagtttctg tggttgatgt gaaagatatg atgtctgaat caggcaacaa
    17521 tccaatatta gcattgcaag gacgagttgc aggtatgtct attactgcag atggaaatcc
    17581 tagtggcgct gccacagtgc gtattcgtgg tattggtacc atgaacaata atgatccgct
    17641 ttacgtcatt gacggtgtac ctaccaaagg aggaatgcat gaactgaaca gtaacgatat
    17701 agaatcaatt caggtactac gcgatgcctc ggcagcgacc atttatggtt cacgtgcggc
    17761 aaacggtgtt atcattatta caactaagca aggggaaaaa gggaaaacga acgttaattt
    17821 cgacagttat ttaactttgt cccaatatgg taaaacggta gaaatgatga acactaaaca
    17881 atacggtgaa agcttgtggc aggcaatgat aaatagtgga tatgatccta atgctaatgg
    17941 tatcggttat atgtataact acagttatga tgac
//
LOCUS       DAOSCH010000035        17881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2186_length_17881_cov_5.137496, whole genome shotgun sequence.
ACCESSION   DAOSCH010000035 DAOSCH010000000
VERSION     DAOSCH010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17881
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2186_length_17881_cov_5.137496"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="PLZ54_04245"
     CDS             complement(<1..859)
                     /locus_tag="PLZ54_04245"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HOJ66175.1"
                     /translation="MKKRFFLVLCCFFGFLFSEAQIFEPIHWDISHKLTGKTTADIIF
                     KASIDEGWHLYGLSLPPNGPKPTAIVFEKIENARKVGELQAVSNLEKKFDPNFGMELN
                     WYTKEAVFIQKITFSSMQNVRLEGYVEYMACNDKSCLPPTSEPFSIRLSTTEFNSINS
                     GEQAPSSESEKIQPTEDFKNAKQVDLSSEDTAVTSSEWWTPVVKELNSFGNVVYHKET
                     TSLWYILLAGLLGGFLALLTPCVWPIIPMTVSFFLKRSAVPSRGRKDAVLYGLSIIVI
                     YVTLGLLITL"
     gene            complement(972..8405)
                     /gene="sprA"
                     /locus_tag="PLZ54_04250"
     CDS             complement(972..8405)
                     /gene="sprA"
                     /locus_tag="PLZ54_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOJ66176.1"
                     /translation="MTNRKKSPSIFVYFGIFLSSTVGFVYASLQQPVPAENRSNAVKQ
                     QDSVQQHFPVKKLVQDTYEDITAAYPLDAPVPSNIKTEIEYDPSTASYILHTYLGNIE
                     IATPFRMTEQEYLDYSGRQEMQRYWYEKNSSAQQNVEDKFSVTDMKFDIGPADKIFGP
                     GGVQIRTQGSAEISMGFKTNKIDNPALTERMRKSTILDFDEKIQMNVNGKVGDKVNFN
                     MNYNTESSFDFDQKLLKLNYKGKEDDIIKNIEIGNVSMPLNSSLITGSTALFGVKTDL
                     QFGKLSISAVASQQQSQTQTVSSKGGAQTTDYEIGIDEYDENRHFFLGHYFRNNFESA
                     MSKLPFVTSGVTITRVEVWVTNKRGNYDQARNIVAFMDLGETQRLDNHHWNVLSATSL
                     PQNKANSLYDEVTSIPNVRDIQQTNNVLSSQYAADSIFGGEDYEKIESARRLDPSEYT
                     LNSSLGYISLRTALNPDEVLAVAYEYTYGGQVYQVGEFSTDAVTAPNALIVKLLKSTA
                     QSPSLATWDLMMKNVYSLGALQIQPENFQLDIVYRNDSVGTDLKYITEGNIQNQLLLR
                     VMNLDRLNTKNAAQPDGKFDYVEGYTVLSSSGRIIFPVLEPFGSHLRKAIGNDQIAEK
                     YVFQELYDSTLVTAREYSEKNKFHIVGEYKASSGNEIRLNAMNIPRGSVTVTAGGVAL
                     VENVDYTVDYTMGTVNIINTSILESGSNIDVKLENQSMFSMQRKSLVGTHLEYQFNRD
                     FSLGATVMHLSEMPLTTKVNTGDEPISNTIWGLNTSWRKESQWLTNQLDKLPFVNATK
                     PSTLALNAEFAQLIPGHSNVIGSAGLAYIDDFESTKTTIDLHYPVFWHLASTPFDPNG
                     GLFPEAALSNNVDYGKNRALLAWYYVDPILNANSRNTPENLRNNLESQSNHLTRNVLE
                     EEIFPNKERLATQTSRMTIMNLSFYPNERGPYNLDWEGINEDGSLKNPEKRWGGIMRK
                     LDVTDFENSNIEYIEFWMMDPFITDKDGMQKGGDLYINLGDISEDILKDGKKFFEQGL
                     SIDGDVTKSDTTVWGRVPKTQSTVKAFDNTPGARKYQDVGLNGLSTDEEFEYPTYKKY
                     VEQITRKISPQTLQKWQDDQFSPLNDPAGDNYHFYRGSDYDEQKKDILSRYKHYNGTE
                     GNSPEAENVNETYTTSATSLPDMEDLNEDNTMNEYEKYYQYRVQIRRDAFQVGKNYIA
                     DKITSNVKLANGNVEPVTWYQFRIPIREYNDKIGSIRNFKSIRFIRMFLTDFEKDIHL
                     RFATFDLVRGEWRSYTKSLYPNGLTPPSEGKLEVQAVNIEENADRTPVNYVLPPGVTR
                     ETDPGQPQLLQLNEQSMVLRVRDLAPNDAKAVYKTTSYDMRQYKRLQMFVHAEKLIDD
                     ISNLQDNELTCFIRIGTDMVNNYYEYEIPLRLTPHGKYLGENTEQREIVWYPENMFDF
                     AFSKLINVKLQRNKTLQASAGNVSNLVPYTIFDPDKPKNRITVVGNPTISDVQNIMIG
                     VRNRSGSIKSGEIWVNELRMSEFDESGGWAAMGNLAVGLSDIGTLNLSGRIETSGYGS
                     LESNVMNRNLEDLRQMNFAAAIELGRFLPERAKLQIPTYFSYSNETVSPKYNPLDQDV
                     ELSDALDNLPTKSDRDSLLNLSQTVSTSKSFNVANAKLNIKSKKPQFYDPANLKFTYA
                     YIENNEHNSEIEQNLVKNQRAAVDYNFNFAAQPWEPFKDVKALNSPAFKIIKDFNLYY
                     LPSSLNYNANLNRSYSQIKLRSFDVYSGSYTNDELSNLTFSKDFMFNRQFDIKYDLTR
                     SLHFSFQTVMNANIDEPYFTPEMGKEYYEAWRDTVWHNIKKLGTPYVYQQVFTASWNV
                     PLNKIPITDWMQANVSYNSNYNWNRSAQMEGGANIGNTATSTGSWQFDGQLNFETLYN
                     KSKFLKNVLNRNKVTSARPQPFKPGNYTQTVQLKSDQPLTIQHRLGSERIRLTAKDKD
                     GNSVLLSYKVKNASSVEILPKQNMDSITINIVSLDPNQQSVINKTRDITLGALMMVRR
                     FSINYRENNSTVLSGFMPETGFMGQQNYNGIDAPGYAFAFGFQEKDFIKKAFERGWLN
                     AGDSVVNPATTAFTSDLDIKATLEPIPGFKIDLNGRRYTASNTSVFYMYDGMPTTYSG
                     SFNITQIGLSTAFKKLGNATDNYNSETFNAFLANRQKVADRLNQKYVGTRYPQSGFIK
                     SSNLAGKEYDPSLGAYQLNSADVLIPAFLAAYTGRDATKISTNPFLSISSILPNWRIS
                     YDGLSKIEWFKNHFRNVNLTHAYSFRYSIGSYSSYSTWVPMEDESSLGYVRDVQTDNP
                     IPSGAYDFTSVSLNEQFSPLLGINATMNNSLSAKIEYRKQRNLALNLSSTQLIESASD
                     EYVLGFGYLLKDFDVILRLKSDKQSKIKNDLKLSADISYKDIKTLLRKVEENITQASS
                     GNKMITIKFLADYVFSSKVNIQLYYDRQSSTPLVSSSFPVSSSNFGASLRFMLTR"
     gene            complement(8485..9075)
                     /gene="ruvA"
                     /locus_tag="PLZ54_04255"
     CDS             complement(8485..9075)
                     /gene="ruvA"
                     /locus_tag="PLZ54_04255"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016278555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOJ66177.1"
                     /translation="MIDYIKGQIVELTPTYVVLEANGLGYFISIPLPAYTLLSHQSEA
                     KLFVHEVVREDAYLFFGFITKKEREVFQLLISVSGIGVNTARMIMSSYSVEEIQEIIA
                     AGNVSALNAIKGIGSKTAQRIIVDLKDKILKGKDDGGSLSLLSDKNAQIKEEAVSALV
                     MLGFPAGASQKAIENILKEQSQLEVEQLIKMALKIL"
     gene            9346..10020
                     /locus_tag="PLZ54_04260"
     CDS             9346..10020
                     /locus_tag="PLZ54_04260"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOJ66178.1"
                     /translation="MKSLTLSFIALFGAMVMAFGQFQLPKLDYGYSDLEPYIDSTTMY
                     IHYNNHHATYANNLNKTLEKYPELYKKDIVELLENINELPADIQLSVRNNGGGYYNHC
                     LFWSMLAPAGSTTISPKLEKILIKNFGSIDTFKNEFEKAALGQFGSGWAWLIKESSGK
                     LRIISTSNQDNTLMPFSSVKGKPVLTLDVWEHAYYLKYQSKRASYVKAFWNVVNWKEV
                     EKLIEK"
     gene            complement(10334..11029)
                     /locus_tag="PLZ54_04265"
     CDS             complement(10334..11029)
                     /locus_tag="PLZ54_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275865.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOJ66179.1"
                     /translation="MKNRIVEAAIIAVGIICLGLCIRNGIKIFSQKDRAVEVKGLAEM
                     EVPANKVICPILYKDLGNDLAQLYVDMNKKNQAIISFIKENGIQENEISPSAPEIVDM
                     AAERFIENKSPYRYNLTSVITISTPNVDKVLALMSRQAELLKQGIAVTVNNYSVRYEY
                     TKLNEIKPQMIEEATKNARKAAEKFAKDSGSKLGKIKSATQGQFIISDRDENTPYIKN
                     VRVVSTITYYLED"
     gene            complement(11099..11839)
                     /locus_tag="PLZ54_04270"
     CDS             complement(11099..11839)
                     /locus_tag="PLZ54_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ66180.1"
                     /translation="MKKLIIISLPLILLLFSMKASSQKFEKDVFPTSAGNLTITFIGH
                     GTLMFEIDNKVIHIDPTAMFADYSTLPKADLILITHEHADHFDMATIDKISKKETEII
                     LTTTCKNQLQKGKVMKNGDSYNWKKFIHIQAVPAYNIQHKTNTGQLFHPKGQGNGYIL
                     TFGNFKVYVAGDTENIPEMKSLKDIDVAFLPMNLPYTMTPKMTADAALSFMPKILYPY
                     HYGNTNTNELVNLLKNRPEIEVRIRKLN"
     gene            complement(11926..13383)
                     /gene="rodA"
                     /locus_tag="PLZ54_04275"
     CDS             complement(11926..13383)
                     /gene="rodA"
                     /locus_tag="PLZ54_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOJ66181.1"
                     /translation="MARTSNIKYDWITIIYYVLLVFMGWLSIYGASYDFEHPAIFDFS
                     QRAGKQFIWILTAFGIAGILLLIDDRIYNYFSYFIYGFTILLLIATIFVAQDIKGSRS
                     WIVIGPISFQPAEIAKMSTSLALAKFMNTYGFKLRSWKNLIPLLVIVFIPLILIMLQN
                     ETGSALVFLSFFLMFYREGMNGIVLLIGALAAFFFVVVIRFSIVPIAFGTGTLGIVLA
                     MTLIIVGQFIYAYFFVKNKKEALYLLGGSSLVALVAYLLSLKFKVNFNSVAFFTVLAS
                     CVYWMVMELKFRHKKFLYLSLISLGFSLFSFSSDYIFEKTLEPHQQTRIKVLLNMEND
                     PMGAGYNINQSKIAIGSGGFWGKGFLNGTQTKLKYVPEQDTDFIFCTVGEEHGFVGST
                     IVLILYWLFLMRLISIAERQRETFNRVYAYCVVSIFFFHIMVNIGMVLGIMPVIGIPL
                     PFFSYGGSSLWGFTILLFILLRLDASRFEHIDTNQ"
     gene            complement(13367..15247)
                     /locus_tag="PLZ54_04280"
     CDS             complement(13367..15247)
                     /locus_tag="PLZ54_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOJ66182.1"
                     /translation="MINDSLQNRRYVIAGIISVVIALYIIRLFSIQVIETKYKQGAVS
                     NAFLNKTLYPPRGLIYDRNQNLLVYNKPAYDIAVIMREIHDLDTLAFCRALNIDQTFF
                     NKRMAEIKDRKKNLGYSAYTPQLFLTQLDIQDIATIQQMMYKFPGFYIQSRTLREYAY
                     PYAAHVLGSIGEISQSRLDEDNYYVRGDYVGRDGLEYTYEKDLRGEKGVEVLLRDAKG
                     RIKGKYENGKHDVAPKTGKNLITTLDIQLQMLGEQLLNGKIGGIVAIEPKTGEILAMV
                     SSPTFNPSSLVGRQRTKNYIKLANDPAKPLLNRATQAQYSPGSTFKTIQALVCQQMGG
                     IDTKTLFPCNGPASSPIKCTHHHGSPVNLLNAIEQSCNPYFWGAFRTTLEKDGYGEKN
                     KNFKRNYQIWRSYVLSFGFGKKFTDGDIYQQSSGNIPTEKYFNRYFGETGWRAMTIRS
                     LAIGQGEILVTPLQLSNAMAIIANKGYFVTPHLNKADSLLNRVHRTSVEKNYFDIVDE
                     GMWRVCEFGTARHYKVPNLSMCGKTGTVQNSRGKDHSIFVGYAPRENPQIAIAVVVEN
                     AGFGATWALPIASLMIERYINGKIERTDLLERIASATLSASATRLTNKTNLTDFINGQ
                     DF"
     gene            complement(15251..15751)
                     /gene="mreD"
                     /locus_tag="PLZ54_04285"
     CDS             complement(15251..15751)
                     /gene="mreD"
                     /locus_tag="PLZ54_04285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOJ66183.1"
                     /translation="MAITVFQNIIRFVLLMMVQIFVLNNIQLSGYINPYIYILFILSL
                     PYRTPQWLTLILAFVTGLMVDIFMNTMGTHAFATVLMAFTRNGIINLITDFEKDENPT
                     PSFYTFGIGVYIRYIVLLVFIHHTALFFLEAFSLAHFGIVIKKIFFSSVVSILLILGI
                     QSLKKK"
     gene            complement(15753..16598)
                     /gene="mreC"
                     /locus_tag="PLZ54_04290"
     CDS             complement(15753..16598)
                     /gene="mreC"
                     /locus_tag="PLZ54_04290"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOJ66184.1"
                     /translation="MKNLINFLIHHSGFFVFLLLEILSLMLVFNNRDYQKSVFFSSSN
                     TIISSFYNWNNYIVGFFNLSKSNQQLAEENTQLKNQLLDLQNKLEALQPSGTDSLSYK
                     IPPEKQYRFIEAKVINNSTNKLQNYITLNKGALDGVKPDMGVINDEGIVGIVKNVSQH
                     FSVVIPVLNPKIQINCKFKRNNYIGSLVWDGKDYRFALLTDIARHVPIKMGDTLVTSG
                     LTTTFPEGIPVGTIENFNLKESDAYYEIKVKLATNFRTLSYVKVIDFKNAGELKELEK
                     AAYEE"
     gene            complement(16710..17729)
                     /locus_tag="PLZ54_04295"
     CDS             complement(16710..17729)
                     /locus_tag="PLZ54_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOJ66185.1"
                     /translation="MGFFSFTQEIAIDLGTANSIIVYNDQIVVDEPSVVALDKRTDRL
                     IAVGERARQMQGKENESIRTIRPLRDGVIADFYAAEMMIRGLIKMIPSKNRFFTPSLK
                     MVIGIPSGSTEVEMRAVRDSSEHAGGREIYMIYEPMAAALGIGIDVEAPDGCMVVDIG
                     GGTTEIAVISLGGIVLNKSIRIAGDDLTDDIVEYMGRMHNIKIGERTAELIKINVGAA
                     LTDLEDAPEDYIVRGPNRMTALPIEVPVSYQEIAHCLEKSISKIEAAILSALELTPPE
                     LYSDIVQHGIFLSGGGALLRGLDKRLTDKINIPFHIADDPLHAVARGTGIALKNVDKF
                     SFLLR"
BASE COUNT         5391 a         3606 c         3343 g         5541 t
ORIGIN      
        1 taagcgttat caacaatccc aatgtcacat aaatgactat aatggacaaa ccatataaaa
       61 ctgcatcttt tcttccgcgt gaaggaacgg cagaacgttt caggaagaaa cttaccgtca
      121 tgggaataat tggccaaaca catggagtaa gcaaagccag aaatccgccc aataaaccgg
      181 ccagcaaaat gtaccaaagc gaagtcgttt ccttatgata aaccacattc ccaaagctgt
      241 tcaactcttt cactaccggt gtccaccatt cagaagaagt tacggcagta tcttcggaac
      301 ttaaatctac ttgtttggca tttttgaaat cttccgttgg ctgaattttt tcggattccg
      361 aagaaggtgc ttgctccccg gagttgatgg aattaaattc agtggtactc aatcgaatcg
      421 aaaacggttc ggaagtggga ggcaaacaac ttttgtcgtt acaggccatg tattctacat
      481 acccttccaa acgaacattt tgcatggaag agaatgtaat tttctgaatg aaaacagcct
      541 ctttcgtgta ccagttcagc tccatgccaa agtttggatc gaactttttc tccaagttcg
      601 aaacggcttg caattcacca accttgcggg cattttcaat tttttcaaaa actatagcgg
      661 taggtttagg tccatttgga ggtaaagata acccgtataa atgccaccct tcatctatgg
      721 aagctttgaa aataatgtcg gccgtagttt ttccggtcag tttatgactg atatcccaat
      781 gtatcggttc aaaaatttgg gcttcggaaa acaagaaccc gaaaaagcaa cacagtacca
      841 gaaaaaatct tttcttcatt tcacgtgatt aaaatttcta ttaaccttcc aacaataata
      901 atcaaccaaa gtttaattga ggtttgatat tcaaaaaatt taacaattca acaaacaaca
      961 tgtaatcact tttatctggt cagcataaac ctgagactag ccccaaaatt ggaagatgaa
     1021 accggaaatg aagaggaaac aagtggggta ctcgactgac ggtcataata aagttgaata
     1081 ttgactttag aactgaatac gtaatcggcc aggaatttta tggttatcat cttatttcca
     1141 ctcgaagcct gcgtaatatt ctcctccact ttgcgcaaca aggttttaat atctttatag
     1201 gaaatatcgg ccgaaagttt taaatcgttt ttgattttcg actgcttgtc cgatttcaat
     1261 cgcaaaatca catcaaaatc cttcaacaga taaccaaatc ccaaaacata ttcgtcgctg
     1321 gcactttcaa taagctgagt gctggaaagg ttcaaagcaa ggtttctttg cttgcggtat
     1381 tcgatttttg ccgacaaact gttgttcatg gtagcattga tacccagaag tggactaaac
     1441 tgttcgttga gcgaaacact agtaaaatcg taagcacccg aaggaatcgg attgtcggtt
     1501 tgaacatcgc gaacataacc caacgaactt tcgtcctcca taggcaccca tgtagaataa
     1561 gaagaatagg aaccaatgct gtaacgaaaa gaataggcat gggtcagatt cacatttcta
     1621 aaatgatttt taaaccattc tatttttgaa agtccatcat agctgattcg ccagttgggc
     1681 aaaatactcg aaatagaaag gaacggattg gtagaaattt ttgtagcatc acgtccggta
     1741 taagcagcca gaaaagctgg aatcagcaca tcggcagaat tcagctgata tgcacccaat
     1801 gatgggtcat attctttgcc tgccaaattg gaagatttga taaatccact ttgaggatat
     1861 cgcgtgccta catatttctg attcagacgg tcggcaactt tttgccgatt ggcaaggaaa
     1921 gcattaaaag tttccgaatt gtaattgtcg gtagcgtttc ccagcttttt aaaggcagtg
     1981 gacaagccaa tttgcgtaat attaaaactt ccgctgtaag tagtcggcat accgtcgtac
     2041 atgtagaaca ccgaagtatt tgaagccgtg tatcgccgcc cattcaaatc aattttaaag
     2101 cccggaatag gttccagcgt tgctttgata tcaagatcgg aagtaaaagc agttgtggcc
     2161 gggttaacca cagaatcacc tgcattcagc caacctcttt cgaaagcttt ttttataaaa
     2221 tctttttcct gaaatccaaa agcaaacgca tatccgggag catcgatacc attgtaattc
     2281 tgctgcccca taaatccagt ttcaggcata aatcccgaca ataccgtgct gttgttttct
     2341 ctataattga tagaaaaccg tcgaaccatc attaaagcac ccaatgtaat atcacgcgtt
     2401 ttgtttatta ccgattgctg attgggatcc agactgacaa tgttgattgt gatgctatcc
     2461 atattttgtt tgggtaaaat ctctaccgaa gaagcatttt ttactttata ggagagtaac
     2521 acagaatttc catccttatc ttttgctgtc agcctgatgc gctcactgcc caagcgatgc
     2581 tgaatggtga gcggctggtc ggacttgagt tgaacggtct gagtataatt tcccggtttg
     2641 aaaggctgag gtctggcact agtaacttta tttcggttca aaacgttttt cagaaacttg
     2701 gatttattgt aaagggtttc aaaattcagt tgtccatcaa actgccacga accggtgctg
     2761 gtagctgtat ttccaatatt ggctcctcct tccatttgtg cacttcgatt ccagttgtaa
     2821 ttggaattat aggaaacatt tgcctgcatc cagtctgtga tgggtatctt attgagtgga
     2881 acattccaag aagcagtaaa tacctgctga taaacatagg gtgttccaag ttttttgata
     2941 ttgtgccaca ctgtatcgcg ccatgcttcg taatactcct tccccatttc gggcgtaaaa
     3001 tagggttcat cgatgttggc gttcattact gtttgaaaac tgaaatgtaa cgaacgagtc
     3061 aaatcatatt tgatatcgaa ctgccgattg aacataaaat ccttgctgaa agtgagattg
     3121 gacaattcat cgttggtata gctgcccgaa taaacatcaa aactccgaag tttgatttga
     3181 gaatacgatc gattgagatt tgcgttataa tttaaagaag aaggcagata atacagatta
     3241 aaatccttga tgattttaaa tgccggactg ttcaaagctt ttacatcttt aaacggttcc
     3301 cacggctggg ctgcaaagtt gaaattataa tcgactgcag cacgctggtt ttttaccaaa
     3361 ttttgctcaa tctccgaatt atgttcattg ttttcaatat aggcataggt aaacttcaaa
     3421 tttgcagggt cataaaactg tggtttttta ctttttatat ttaatttggc attggcaaca
     3481 ttaaaacttt ttgaagtgct caccgtttgc gacaaattca gcaaggaatc ccgatctgat
     3541 tttgtgggaa gattatccaa agcatcggac agttcgacat cctgatcgag cggattatat
     3601 tttggcgata cggtttcgtt ggaatacgag aaataagtag gaatctgcaa tttagcccgt
     3661 tcaggtaaaa atctgcccaa ttctatggca gctgcaaaat tcatctgacg caaatcttcc
     3721 agatttctat tcataacatt actttcaagg ctcccataac ccgaagtttc aattcttccc
     3781 gaaagattca gtgtcccaat gtccgataat cctaccgcca aatttcccat tgcagcccag
     3841 ccacccgatt catcaaattc agacatgcgt aattcgttta cccaaatttc tcccgatttt
     3901 atgctgccac ttctgttgcg aacgccaatc ataatattct gaacatcgga aatggtagga
     3961 tttcccacaa ccgtaattct attttttggc ttatcgggat caaatatggt ataaggcaca
     4021 agattcgaaa catttcctgc gctggcttga agagtcttgt tgcgttggag tttgacatta
     4081 atcagttttg aaaatgcaaa atcaaacatg ttttcaggat accatacaat ctcccgctgc
     4141 tcggtatttt cacccagata ttttccgtga ggcgtcaatc gcaagggaat ttcgtattca
     4201 tagtaattgt tcaccatatc ggttccgatt cggataaaac aagtcaattc gttatcctgc
     4261 aaattggaaa tatcgtcaat caatttttct gcatgtacaa acatttgaag acgcttgtac
     4321 tgacgcatat cgtatgaagt agttttataa acggctttgg catcatttgg cgccaaatct
     4381 ctgactctta aaaccatcga ttgttcgttc aactgtaata gctgtggttg acccggatca
     4441 gtttctctgg taactccagg aggcaataca taatttactg gggtacgatc ggcgttttct
     4501 tcaatattca cagcctgaac ttccaatttc ccttccgaag ggggagtcag tccattcgga
     4561 tacaatgact tggtgtaact tcgccattca ccccttacca aatcgaaagt agcaaaacgc
     4621 aaatgaatgt ctttttcaaa atcggtaaga aacattctga taaaacgaat ggatttaaaa
     4681 ttgcgaatgc tcccgatttt atcgttgtac tcacggatgg gaatacggaa ctgataccaa
     4741 gttacaggtt caacgtttcc attggccaac tttacattgg aagtaatttt gtcggctata
     4801 taatttttcc ctacctgaaa agcatctctg cgaatctgaa cccgatactg ataatatttt
     4861 tcgtattcgt tcatggtatt gtcttcattc aaatcttcca tgtcgggaag cgaagtagct
     4921 gaagttgtgt aagtttcgtt aacattttct gcttcaggtg aatttccttc cgtcccatta
     4981 taatgcttgt atcgggaaag aatatctttc ttctgttcgt cataatcgga accacgataa
     5041 aaatgatagt tgtcgcctgc aggatcattc aatggcgaaa attggtcgtc ttgccatttt
     5101 tggagggttt gaggactgat ttttcgtgta atttgttcta catatttttt gtaagtagga
     5161 tattcaaatt cttcatcggt ggatagtcca tttaaaccaa catcctgata ttttctcgct
     5221 cccggtgtat tgtcaaaagc ctttaccgtt gattgcgttt tcggaactct tccccacacg
     5281 gtagtatcgc ttttggtaac atcgccatcg atactcaagc cctgttcaaa aaactttttc
     5341 ccatctttta aaatatcctc gctgatatcc cccaaattta tatacaaatc accacctttc
     5401 tgcatgccat ctttatcggt aatgaaagga tccatcatcc aaaattcgat atattctatg
     5461 ttggaatttt caaaatccgt tacgtccagt ttacgcatga taccacccca tcttttttcg
     5521 ggatttttca atgaaccatc ttcatttatg ccttcccagt ccagattgta cggacctctt
     5581 tcgttaggat aaaaggaaag attcataatg gtcattcggg atgtttgcgt tgcaagcctt
     5641 tctttattcg gaaaaatttc ttcttccaac acattgcgcg ttaaatgatt ggattgtgat
     5701 tccagattgt ttcttaaatt ttcgggagta ttacgactgt tggcattcaa tatcggatcc
     5761 acatagtacc atgccaataa ggctctattt ttaccataat ccacattgtt gctcagtgcg
     5821 gcttcaggga aaagtccacc attggggtca aaaggcgtgc tggcaagatg ccagaatacc
     5881 ggataatgaa ggtctatggt ggttttggtc gattcaaaat cgtcgatata ggccagccct
     5941 gccgaaccaa taacatttga atgtccggga atcaactggg caaattcggc atttagcgcc
     6001 aatgttgaag gcttggtggc attaacaaaa ggcagcttgt cgagctggtt ggtgagccat
     6061 tgagattcct ttctccacga agtattgagc ccccaaatgg tattggaaat gggctcatct
     6121 cctgtgttga ctttggtggt caaaggcatt tcggaaagat gcatcacggt ggcgcccaac
     6181 gaaaaatcgc gattaaactg atattccaaa tgggttccta ccaacgattt tcgttgcatg
     6241 ctgaacatcg actgattttc caatttgaca tcaatattgc tacctgattc caaaatggaa
     6301 gtattgatta tatttaccgt tcccatagta taatcaactg tataatcaac attttctaca
     6361 agagcaacac caccggccgt taccgtaacc gagcctcgtg gtatattcat ggcattcagt
     6421 cgaatttcgt ttcccgaact ggccttgtat tccccaacga tgtgaaattt gtttttttca
     6481 ctgtattcac gagcggttac caatgtagaa tcatacaatt cctgaaaaac atatttttcg
     6541 gcaatctgat cattgccaat cgctttccgc aaatgggaac caaaaggttc gagcacaggg
     6601 aaaataattc ttccgctgga agaaaggaca gtgtagcctt ccacataatc aaattttccg
     6661 tcgggctgag ctgcattttt tgtgttaagc cgatcgagat tcatcacacg cagcaaaagc
     6721 tgattttgta tgtttccttc ggtaatatat ttcagatcgg ttcccaccga atcgtttcga
     6781 taaacaatat ccaactgaaa attttcaggt tgaatctgca gagcgcccaa actatacacg
     6841 tttttcatca tcaaatccca agtggcaagt gaaggagact gggcagtact tttcaaaagt
     6901 ttaacaatca aagcattcgg agcagttaca gcatcggtag aaaattctcc tacctgataa
     6961 acctgtcctc cgtaagtata ttcataggca acagccaaca cttcatcggg attcaaagcg
     7021 gtacgtaacg aaatataacc caaagaagaa ttcaaggtgt attccgaagg atcgagtctt
     7081 cgggcacttt caattttctc ataatcttct cctccaaaaa tactgtcggc agcatattga
     7141 gaagaaagta cattgttggt ctgctgtatg tcccgaacat taggaataga agtaacttca
     7201 tcataaagag aattggcttt gttttgtggt aaagatgtcg ctgaaagcac attccaatga
     7261 tgattgtcaa gtcgctgtgt ttctcccaaa tccataaatg caacaatgtt ccttgcctga
     7321 tcgtaatttc cacgtttgtt ggttacccaa acttcaacac gtgtaatggt aacacccgaa
     7381 gttacaaatg ggagcttgct catggcactt tcaaaattgt ttctgaaata atgccccaaa
     7441 aagaaatgtc ggttttcatc atattcatca atacctattt cgtaatcggt agtttgtgct
     7501 cctccttttg aactgacagt ttgcgtctgc gattgttgct gagaagctac ggcgctaatg
     7561 cttaacttgc caaactgcaa atcggtttta acaccaaaca atgccgtgct cccggtaatg
     7621 agagaactgt tcaacggcat gctgacatta ccaatttcaa tgtttttaat gatatcatct
     7681 tccttgcctt tataattcag ttttaacaat ttctggtcaa aatcgaaact cgactcggta
     7741 ttgtaattca tattgaagtt caccttgtcg ccaaccttgc catttacatt catctgaatt
     7801 ttctcgtcaa agtcgagaat agtacttttg cgcatccgct cagtcaatgc cggattgtcg
     7861 attttgttgg ttttgaaacc catagaaatt tcggcagaac cttgtgttct gatttgaact
     7921 ccaccgggcc caaatatttt atcggcaggg ccaatatcga atttcatgtc cgtaacactg
     7981 aatttgtctt caacgttttg ttgtgcagaa gaattttttt cgtaccaata acgttgcatt
     8041 tcttgccttc cggaataatc gagatattcc tgttccgtca ttcgaaacgg agtagctatt
     8101 tcgatgtttc ccaaataagt atgcaaaata taactggctg ttgatgggtc gtattcaatt
     8161 tccgttttaa tatttgaagg aacgggtgcg tcgagaggat aagctgcagt gatatcttcg
     8221 taggtatcct gcaccaattt tttgacagga aaatgttgct gaacggaatc ttgctgtttc
     8281 acagcattgg aacgattttc ggccggaacg ggttgttgca gcgaagcata aacaaatcca
     8341 actgtcgaac tcaaaaaaat accgaaataa acaaaaatcg aaggtgattt ttttctattt
     8401 gtcatataaa atttctattc cttgttctta acaatttgtc ttcactgaaa tgaagttttc
     8461 ttgaatttca aattttgcag accctcacaa aattttcagg gccattttga tcaattgttc
     8521 aacttcaagc tgagactgtt ctttcaagat attttctatt gctttctgcg aagcaccagc
     8581 cggaaaacca agcatgacca atgcagacac tgcttcttcc tttatttggg catttttgtc
     8641 ggaaagcaaa gacagcgaac caccatcatc tttccctttt aaaattttgt ccttcaaatc
     8701 cacaataata cgctgggccg ttttgctgcc aatgccttta atggcattca gcgccgacac
     8761 attgccggcg gcaattattt cctgaatttc ttctaccgaa taagaagaca taatcattct
     8821 ggcagtattt acgcctatgc ccgaaacgga aattaaaagt tgaaaaactt ctctctcctt
     8881 tttggtaatg aaaccaaaaa acaaataagc atcttcccgc actacttcat gcacaaaaag
     8941 ctttgcttcc gattgatgac tcaataaagt ataggccggc aaagggattg aaatgaaata
     9001 tcccagtccg tttgcctcca gcaccacata agttggggtc aactcaacta tttgcccctt
     9061 aatataatca atcatccgac aaaactttaa aaaagagttt ttgtgtgtaa aaatacaaat
     9121 ttatttacat acgctccaaa agacaacctc caacccattg acagaagtac tgattaaaat
     9181 aacgctgtat ttgattaaaa attacaatta caacgttaaa aggaagaaaa gccgacttcc
     9241 atgatggtaa aaaatggcat caaccttata gatttttttt catcactgaa caaaggaaat
     9301 aagtctgttg tttataaata taataactct aaaaacatta taaaaatgaa atccttaaca
     9361 ttatcattca tagcattatt tggagctatg gtcatggctt tcggacaatt tcagcttcca
     9421 aaacttgatt atggctacag cgatcttgaa ccgtatattg acagtacaac catgtacatt
     9481 cactacaaca accatcatgc gacttacgca aacaatttga ataaaacact cgaaaaatat
     9541 cctgaacttt acaagaagga tatcgtagaa ttgttggaaa atataaacga attgcctgct
     9601 gatattcaat tgtcggtacg caacaatggt ggaggttatt acaatcattg tcttttctgg
     9661 tcgatgctgg caccggccgg ttccacaacc atttctccca aactcgaaaa aatactgatt
     9721 aaaaatttcg gcagcatcga tacttttaag aacgaatttg aaaaagctgc attaggacaa
     9781 tttggttcag gatgggcctg gctgataaaa gaatcttcag gaaaattgag aatcatttcc
     9841 acttccaatc aggataatac cttaatgcca ttttcttcgg taaaaggaaa gccggttttg
     9901 acgctcgatg tgtgggaaca tgcgtattat ctgaaatatc agtcaaaacg agcatcctat
     9961 gtcaaagcct tttggaatgt ggtaaattgg aaagaagttg aaaaactgat tgaaaagtaa
    10021 atttcttcac tttgattttt ttaaaaaaaa ggtaatatgc taaacgttac ctttttttgt
    10081 gcatgtcgtc aataacacaa gttttcgaga acaacgattt tttcgctttt cagtttactc
    10141 tattttagca gttttagtaa acaaattggt tctgagataa attcaattaa cgttattttc
    10201 aagaagttat cttctacaga ttgcttattg ctgccgtaaa ctacactccg atttcaagtc
    10261 aagaatttac aataaaaatc attgcggata atcattataa tatatcgcaa tacggataat
    10321 tataaaaaat tttttagtct tccaaataat aggttatggt agaaacaacg cggacatttt
    10381 ttatgtaagg cgtgttctcg tctctgtcgg aaataataaa ctgaccctga gtggcgcttt
    10441 taatttttcc taatttgctc cctgaatcct tggcaaattt ttcggctgct tttcgagcgt
    10501 tttttgttgc ctcctctatc atttggggct taatttcatt cagtttcgta tattcgtatc
    10561 gaacgctgta attgttgacc gtaacagcta ttccttgctt gagaagttca gcttgacgcg
    10621 acattaaagc aagcacctta tccacatttg gtgtgctaat tgtaataact gaagtcaaat
    10681 tgtatcgata aggcgattta ttttctataa atctttcggc tgccatgtcc acaatttccg
    10741 gtgccgaagg tgatatttca ttttcctgaa ttccgttttc ctttatgaaa gaaataatgg
    10801 cctgattctt tttattcata tctacataaa gctgagcaag gtcatttccc aaatccttgt
    10861 aaagaatggg gcaaatcacc ttgttggcag gaacttccat ttctgcaagt ccttttactt
    10921 ccactgcgcg gtctttttgt gagaatattt taatgccgtt tcggatgcag agtcccagac
    10981 aaattattcc aacagcaata atggctgctt caacgattct gtttttcatg gaattgattt
    11041 tttaatttgt gaaacaatat tgtttgttta gaaaaaataa gttctttaga aatcaatctt
    11101 aattcaactt gcgaatcctc acttcaattt cgggtctgtt tttcagcaga tttaccaatt
    11161 cgtttgtgtt ggtattaccg taatgatagg gatataaaat ttttggcata aagctaagag
    11221 ccgcgtcggc ggtcattttg ggtgtcattg tgtagggcag gttcatgggt aaaaaagcca
    11281 catcaatatc tttcaaagat ttcatttcag gtatgttttc ggtgtcgccg gccacataaa
    11341 ctttaaaatt tccaaacgtt aatatataac cgtttccttg ccctttggga tggaagagtt
    11401 ggccagtatt tgtcttgtgc tgaatgttgt aagccggaac agcttgaata tgaataaact
    11461 tcttccaatt gtaagagtcc ccgtttttca taacttttcc tttttgcagt tgatttttgc
    11521 aggttgtggt aagaatgatt tcggtttcct ttttactgat tttgtcgatg gtagccatat
    11581 cgaaatgatc ggcatgttca tgtgtaataa gtatcaaatc ggctttggga agagtagaat
    11641 aatcggcaaa catggcagtg gggtcaatgt gtataacttt attgtcgatt tcaaacatca
    11701 atgttccatg tccgataaac gtgattgtta aatttcccgc tgatgtggga aatacgtctt
    11761 tttcaaattt ttgcgatgac gctttcatag aaaataaaag taaaataagc ggcaatgaaa
    11821 tgataattaa ctttttcata atattttttt aatggattta acagaaacga atataagtgt
    11881 taattcaata aattatccag aatatcggcg taaatataaa aaatattatt gatttgtatc
    11941 aatatgttca aatcttgaag cgtcgagtcg caaaagaata aaaagaagaa tcgtaaagcc
    12001 ccaaagtgaa gagccaccat aactgaaaaa tggcaaagga atcccaataa caggcatgat
    12061 gccgagtacc ataccaatgt taaccataat atggaaaaag aaaatactca ctacacaata
    12121 tgcatatacc ctgttgaaag tttcccgctg tcgttcggca atacttataa gtctcatcag
    12181 aaaaagccaa tataaaataa gtactatggt ggaaccgaca aagccatgtt cttctccaac
    12241 ggtacaaaaa atgaaatcgg tatcctgttc gggaacgtat ttcaatttgg tttgggttcc
    12301 gttcaaaaaa ccttttcccc aaaaaccacc tgaaccaatg gcaattttcg actgattgat
    12361 attataaccc gctcccatag ggtcattttc catattcagc aaaactttga tacgggtttg
    12421 ttgatgcggt tcgagtgtct tttcaaaaat ataatctgac gaaaaactga aaagtgaaaa
    12481 accaagtgaa atcagcgaaa gataaagaaa ctttttatgg cgaaatttca gctccatcac
    12541 catccagtat acgcaggaag ccagcactgt gaaaaaagct accgaattga aattcacttt
    12601 aaatttcaga ctaagcagat aggcaaccaa agcaaccaat gaacttccac ccaacaaata
    12661 aagcgcttct tttttatttt taacaaaaaa ataagcatat ataaactgtc caacaataat
    12721 taacgtcata gccaaaacaa ttcccaatgt gcctgtgccg aaggcaattg gaacgatgct
    12781 gaaacgaata accacaacaa agaaaaatgc agctaaagca ccaatcaata aaacaattcc
    12841 attcattccc tctcgataga acatcagaaa aaacgacaaa aaaaccagag ccgatccggt
    12901 ttcattttgc agcataatga gaatcaaagg aataaatacg attaccaaca aaggaatcaa
    12961 gtttttccac gatcgcaatt tgaaaccata tgtgttcata aacttggcca acgcaagaga
    13021 tgtggacatt tttgcaattt cggcaggctg aaagctaata ggacctatga caatccacga
    13081 acgcgaacct ttgatgtcct gagcaacaaa aattgtggca ataagcaata aaatagtaaa
    13141 gccgtaaata aagtagctga aatagttgta aattcggtca tcaattagca atagaatgcc
    13201 tgcaatgccg aaagccgtca gtatccaaat aaattgtttc cctgcacgct gactgaagtc
    13261 aaaaattgcc ggatgttcga agtcgtaact tgcgccataa atgctaagcc aacccataaa
    13321 aaccagcaaa acgtagtaaa tgatggttat ccagtcgtat ttgatattag aagtcctggc
    13381 cattgataaa atctgtaaga ttggttttat ttgttaatct agttgcggaa gctgagagag
    13441 ttgccgatgc aattcgttcc agcaaatcgg tacgttcgat ttttccgtta atatatcgct
    13501 caatcattaa actggcaatt gggagcgccc atgttgctcc aaatcccgca ttttccacca
    13561 caacggctat tgcaatttgg ggattttctc ttggcgcata tccaacaaaa atagaatgat
    13621 cttttccccg actgttttgt acggttcctg tttttccgca catggacaaa ttgggaactt
    13681 tgtagtgccg tgccgttcca aattcgcaaa cccgccacat tccttcatct actatgtcga
    13741 aataattttt ttccacgctg gtacgatgaa ctctatttaa caacgagtca gctttgttga
    13801 gatgaggagt aacgaaataa cccttgttgg caataatagc catagcattt gacaactgaa
    13861 gaggagtaac aagaatttca ccctgaccaa tggctaatga tcgaatggtc attgctctcc
    13921 atccggtttc cccaaaatag cgattgaaat atttttcggt tggaatattg cctgaagatt
    13981 gctgatagat gtcgccatcg gtgaattttt ttccaaagcc gaaacttaat acataacttc
    14041 gccagatttg ataatttcgc ttaaaatttt tgtttttttc gccgtatccg tctttttcca
    14101 atgtggtgcg gaaagctccc caaaaataag gattgcaact ttgctctata gcattaagca
    14161 aattaaccgg tgaaccatga tgatgcgtgc atttgatggg agaggatgca ggtccattac
    14221 aaggaaaaag cgttttcgta tcgattcctc ccatttgctg acataccagt gcctgaatag
    14281 ttttaaaggt agaaccgggt gagtattgcg cttgtgtagc cctattcaac aaaggtttgg
    14341 cagggtcatt tgccagtttt atgtaatttt ttgttcgttg ccttcccaca agtgaagaag
    14401 gattaaaagt aggactggaa accatagcca atatttcgcc ggttttgggt tcgatggcaa
    14461 caattccacc tattttcccg ttcaacaact gttctcccag catttgaagc tgtatatcaa
    14521 gcgtcgtaat aagatttttc cctgttttgg gtgccacatc gtgtttcccg ttttcgtatt
    14581 ttccctttat tctgcctttt gcatcacgca gcaagacttc aactcctttt tctcctctga
    14641 gatctttttc gtaggtgtat tccaacccat ctcttccaac ataatcgcct ctaacataat
    14701 agttgtcttc atccaatcgc gattgtgaaa tttctccaat acttcccaaa acatgtgcgg
    14761 cataaggata ggcatattca cgcaaagttc ggctttggat gtaaaatccc ggaaatttgt
    14821 acatcatttg ctgaatggta gctatgtcct gaatatcgag ttgagtcaaa aacaattgcg
    14881 gggtataggc agaatagcca agattctttt ttctgtcctt gatttcggcc attcgtttat
    14941 tgaaaaacgt ttggtctata ttcagagcac gacaaaatgc cagcgtgtcc aagtcgtgaa
    15001 tttcacgcat gatgacggct atgtcatagg caggtttgtt gtaaaccagc aagttttgat
    15061 ttctgtcata aattaaacct ctaggcggat aaagtgtttt attcagaaat gcattgctga
    15121 ctgctccctg tttatatttt gtttctatta cctgaataga gaacaaacga atgatgtaca
    15181 gggcaatgac tacagaaatg atgcctgcaa ttacgtatct tctgttctgt aaagaatcgt
    15241 ttatcattga ttattttttc ttcagcgatt gaatgcccaa aattagcaaa attgatacaa
    15301 ctgagctaaa aaaaattttt ttgataacaa tgccaaagtg agctagtgaa aatgcttcca
    15361 gaaaaaaaag ggcggtatga tgtatgaaaa caagcaatac aatatatctg atatacacgc
    15421 caatgccaaa agtatagaaa gatggagtag gattttcatc tttttcgaaa tcggtaatca
    15481 aattgatgat tccgtttctg gtgaaagcca ttaaaacagt tgcaaaagca tgtgttccca
    15541 ttgtgttcat aaaaatatcc accatgagtc cggttacaaa agcaagaatc aaggttaacc
    15601 actgaggtgt gcgatagggc aacgaaagaa tgaaaagaat gtaaatatag ggattgatgt
    15661 acccacttaa ctgaatgttg ttcagtacga aaatctgaac catcatcagt aaaacgaaac
    15721 gaataatatt ttgaaaaacc gtaattgcca tcttattctt cgtatgctgc tttttcaagc
    15781 tcttttaatt cgccggcatt tttgaaatca atcaccttaa cataggaaag ggtacgaaaa
    15841 tttgttgcca gcttcacttt gatttcataa taggcatcgc tttccttgag gttaaaattt
    15901 tctatggtgc ctaccggaat accttcgggg aaagtagtcg tcagtccgct ggtaaccaat
    15961 gtgtctccca tttttatcgg aacatgtcgc gctatatcgg tcaacaaggc aaagcgataa
    16021 tcttttccat cccatacaag cgaaccaatg taattgtttc gcttaaattt gcaattgatt
    16081 tgaattttgg gatttaaaac cggaattaca acggaaaaat gctgagaaac attttttaca
    16141 atacctacta tgccttcgtc gttgattact cccatatccg gtttcacgcc atccaaagct
    16201 cccttgttca acgtaatata attttgcagt ttattggtag aattgttgat cacttttgct
    16261 tcaataaatc ggtattgctt ttccggaggg attttgtacg aaagcgaatc agtccctgac
    16321 ggttgcaacg cttccagttt attttgcaag tcgagcaact gatttttgag ttgtgtgttt
    16381 tcttcggcca gttgctgatt cgatttgctt aaattgaaaa agccgacaat ataattgttc
    16441 cagttgtaaa aagaagaaat tatggtgttg ctggaagaaa aaaatacact tttctgataa
    16501 tctctgttat tgaaaaccag catcagtgaa aggatttcca acagcaagaa tacaaaaaat
    16561 ccgctatgat gaatcaaaaa attaatgaga tttttcataa aaagtttgta atctttgcgg
    16621 cttttttctg caaacacaaa aatatccgat tctttggagc agtaagaaaa tacattcttg
    16681 tagtccggca gaatcgaata ttaacaattt tatcttaaca agaaagagaa tttatcgaca
    16741 tttttcaaag ctattccggt gccgcgtgct actgcatgaa gcgggtcatc ggcaatatga
    16801 aaaggaatgt tgattttatc cgtgagtctt ttgtctaatc cacgcagcaa agctccgcca
    16861 cccgaaagaa aaataccatg ctgaacaatg tcggaataga gttcgggagg cgtcagttcc
    16921 aatgcgctta atattgctgc ttcaattttt gaaattgatt tttcgagaca atgtgcaatt
    16981 tcctgataag atacaggaac ttcaatgggc aatgccgtca ttctgtttgg tccgcgaaca
    17041 atatagtctt ccggagcatc ttccaaatcg gtcaatgcag ctccaacatt aatttttatc
    17101 aattcggcgg tacgttctcc aattttaata ttgtgcattc gtcccatata ttccacaata
    17161 tcgtcggtca gatcgtctcc ggcaatgcga atagacttgt tcaaaacaat tccgcccaat
    17221 gaaattacgg ctatttctgt tgttcctcca ccaatatcta ccaccatgca gccgtcggga
    17281 gcttccacgt cgattccaat tcccaaagcg gctgccattg gttcgtaaat catatatatt
    17341 tcgcgccctc ccgcatgttc tgaagaatcc ctgacggctc tcatttctac ttcagtgctg
    17401 cctgatggga taccaatgac catttttaag gaaggagtga aaaaacgatt ttttgaagga
    17461 atcattttga taagtccccg aatcatcatt tcggcagcat aaaaatcggc tattacccca
    17521 tcgcgtaaag gccgtatggt acgaatcgac tcgttttcct ttccctgcat ctggcgtgca
    17581 cgttctccta cggctattaa tcggtcagta cgtttgtcca aagccacaac agacggttcg
    17641 tccaccacta tctgatcatt gtaaacgata atgctgttgg cagtacctaa atcgatggct
    17701 atttcctgtg taaatgaaaa aaaacccatt tgtttgtgtg tttatgtttt taagaaaatt
    17761 tttatttgca aattgtcaat gtttgaaatg ccgataacct gtaaatacca tcgaaagacc
    17821 cttttgattg cagaaatcga tggagtcctg gtctttgatg gaaccacccg gttgaatgac
    17881 g
//
LOCUS       DAOSCH010000036        17772 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2204_length_17772_cov_5.867867, whole genome shotgun sequence.
ACCESSION   DAOSCH010000036 DAOSCH010000000
VERSION     DAOSCH010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17772
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2204_length_17772_cov_5.867867"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            146..217
                     /locus_tag="PLZ54_04300"
     tRNA            146..217
                     /locus_tag="PLZ54_04300"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:178..180,aa:Thr,seq:ggt)
     gene            289..1476
                     /gene="tuf"
                     /locus_tag="PLZ54_04305"
     CDS             289..1476
                     /gene="tuf"
                     /locus_tag="PLZ54_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOJ66186.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITKVLAEKGLAEM
                     RSFDSIDNAPEEKERGITINTSHVEYQTAARHYAHVDCPGHTDYVKNMVTGAAQMDGA
                     IIVVAATDGPMPQTREHILLARQVNVPRLVVFINKCDLVDDEEMLDLVEMEMRELLSF
                     YDFDGDNTPVIRGSALGALDGDPKWEAKIVELMDAVDSWIELPQRDVDKPFLMPVEDV
                     FSITGRGTVATGRIETGIVKVGDEVQIIGLGHEAKKTVVTGVEMFRKILDQGQAGDNV
                     GLLLRGIDKDEIKRGMVITHPGAVTPHKKFKAAVYILKKEEGGRHTPFHNHYRPQFYI
                     RTMDVTGEITLPEGVEMVMPGDNIEFTVELIYPVACSVGLRFAIREGGRTVGSGQITE
                     ILD"
     gene            1603..1679
                     /locus_tag="PLZ54_04310"
     tRNA            1603..1679
                     /locus_tag="PLZ54_04310"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1637..1639,aa:Trp,seq:cca)
     gene            1695..1886
                     /gene="secE"
                     /locus_tag="PLZ54_04315"
     CDS             1695..1886
                     /gene="secE"
                     /locus_tag="PLZ54_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOJ66187.1"
                     /translation="MKIINYIKECYSELVHKVSWPSLSELTNSAIVVLIASIILALIV
                     WVMDLSFESIMTFIYEKIF"
     gene            1912..2460
                     /gene="nusG"
                     /locus_tag="PLZ54_04320"
     CDS             1912..2460
                     /gene="nusG"
                     /locus_tag="PLZ54_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOJ66188.1"
                     /translation="MSETTNFKWYVLRAISGKENKVKEYIETEIKNSDLGQYVRQVLI
                     PTEKVFQIRNGKKIAKERNCMPGYVLIEANLMGEALHRVRETPNVLGFLGEKNNIPTP
                     IRTSEINRILWTMDELQESEEEMITPFMVGENVKVIFGPFSGFSGTIEEVNTEKKKLK
                     VMVMIFGRKTPLELAFTQVEKE"
     gene            2514..2954
                     /gene="rplK"
                     /locus_tag="PLZ54_04325"
     CDS             2514..2954
                     /gene="rplK"
                     /locus_tag="PLZ54_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008628200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOJ66189.1"
                     /translation="MAKEIAGVIKLQIKGGAANPSPPVGPALGSKGINIMEFCKQFNA
                     RTQDKAGKILPVVITYYTDKSFDFVVKTPPVAIQLLELTKLKSGSPEPNRKKVAEISW
                     DQVKEIAQDKMVDMNCFELESAMRMVAGTARSMGITVKGTFPGK"
     gene            2976..3671
                     /gene="rplA"
                     /locus_tag="PLZ54_04330"
     CDS             2976..3671
                     /gene="rplA"
                     /locus_tag="PLZ54_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOJ66190.1"
                     /translation="MSTLTKNRKYALEKIEAGKAYPLIEAAKLVKEVTTTKFDASVDI
                     DVRLGVDPRKANQMVRGVVSLPNGTGKQVKVLALCPPELEAAAKEAGADYVGLDEYIE
                     KIKGGWTDVDVIITTPAVMGKLGALGRILGPRGLMPNPKSGTVTNDVAKAIKEVKQGK
                     IDFKVDKYGIVHASIGKVSFTAEQIAENAQEFISTLIKLKPSTAKGTYIKSIYLSSTM
                     SPGIKVDPKSIEI"
     gene            3683..4198
                     /gene="rplJ"
                     /locus_tag="PLZ54_04335"
     CDS             3683..4198
                     /gene="rplJ"
                     /locus_tag="PLZ54_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOJ66191.1"
                     /translation="MKKEDKSTIINQIESVLSEYPHFYLTDIGGLNAAQTSELRKLCY
                     KEKVKLVVVKNTLLKKALDRSSVDFSELYPTLKGETALMLSDMANVPAKLIKNFSAKN
                     KKPVLKAAYVEESFYIGENQLDTLVNVKSKNELIADLIALLQSPAKNVVSALQSGANT
                     IHGVLQTLSEK"
     gene            4237..4614
                     /gene="rplL"
                     /locus_tag="PLZ54_04340"
     CDS             4237..4614
                     /gene="rplL"
                     /locus_tag="PLZ54_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOJ66192.1"
                     /translation="MADLKAFAEQLVNLTVKEVNELAQILKNDYGIEPAAAAVAVAAG
                     PSAAAAEAVEEKTAFDVVLKSPGANKLAIVKLVKELTGLGLKDAKDLVDAAPSVIKEG
                     ISKDEAEALKNQLVEGGAEVEIK"
     gene            4726..8538
                     /gene="rpoB"
                     /locus_tag="PLZ54_04345"
     CDS             4726..8538
                     /gene="rpoB"
                     /locus_tag="PLZ54_04345"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOJ66193.1"
                     /translation="MSSSTNNQRINFASIKNQLEYPDFLEVQVKSFQDFFQMDTSSDK
                     RKSEGLYKVFTENFPIADTRNNFVLEFIDYYIDPPRYSIEECIERGLTYSVPLKAKLK
                     LYCTDPEHEDFDTVIQDVFLGPIPYMTPRGTFVINGAERVIVSQLHRSPGVFFGQSIH
                     ANGTKLYSARIIPFRGSWIEFATDINNVMYAYIDRKKKLPVTTLLRAIGFESDKDILE
                     IFNLAEEIKVNKSNLKKAVGSKLAARVLKTWLEDFVDEDTGEVVTIERNEIIIDRETV
                     LENEHIDLILQSGAQTILIHKEEANQNDYSIIYNTLQKDPSNSEKEAVLYIYRQLRNA
                     EPPDDATAREVINNLFFSEKRYDLGEVGRFRINQKLNLTTSMEVKVLTKEDIIEIIKY
                     LIDLVNSKAEVDDIDHLSNRRVRTVGEQLYNQFSVGLARMARTIRERMNVRDNEVFTP
                     GDLINAKSISSVINSFFGTNALSQFMDQQNPLAEITHKRRMSALGPGGLSRERAGFEV
                     RDVHYTHYGRLCPIETPEGPNIGLISSLCVYAKINELGFIETPYRKVNNGVVDLSENG
                     IGYYTAEEEEDLVVAQGNAPLDENGKFIRSRVKARLGADFPVVDPMEVHLMDVSPSQI
                     ASIAAGLIPFLEHDDANRALMGSNMMRQAVPLLRCEAPIVATGIEGRVIHDSRTQITA
                     EGDGVVEYVDANCIKIRYDRTEEEEFVSFDSAVKEYKLPKYQKTNQNTTIDLRPIVRR
                     GDRVHAGMIITEGYGSESGELALGKNLKVAFMPWKGYNFEDAIVINEKVVREDVFTSI
                     HVVEQLLDVRETKRGTEEFTSDIPNVSEDATRNLDENGIIRVGARVEPGDIIIGKITP
                     KGESDPSPEEKLLRAIFGDKAGDVKDASLKATPSLSGVVIGKRLFSKAQKNRKSKLAD
                     KAVLPKLDEEFETQANALRETLIEKLMKILTNKTSTGVKDFLGTDIIPRNSKFTVERL
                     REIDYTTVQLGKWTNDAHTNDMVNSLITNFLRKFKELDAQLKRQKFNITIGDDLPSGI
                     MQMAKVYIAKKRKIRVGDKMAGRHGNKGIVSRIVREEDMPFLEDGTPVDIVLNPLGVP
                     SRMNLGQIFESVLGWAGKELGVRFATPIFDGATLNDLNAWTDKAGLPRAGKTYLYDGG
                     TGERFDQPATVGVIYMMKLGHMVEDKMHARSIGPYSLITQQPLGGKAQFGGQRFGEME
                     VWALEAFGAANILREILTVKSDDVNGRARTYEAIVKGDPMPTPGIPESLNVLLHELRG
                     LCLSINLE"
     gene            8620..12918
                     /gene="rpoC"
                     /locus_tag="PLZ54_04350"
     CDS             8620..12918
                     /gene="rpoC"
                     /locus_tag="PLZ54_04350"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOJ66194.1"
                     /translation="MAFKTDNKIKSNFSKISIGLASPEEILKLSHGEVLKPETINYRT
                     YKPERDGLFCERIFGPVKDYECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHIQ
                     LVVPVAHIWYFRTLPSKIGYLLGMSSKKLESIIYYEKYVIIQGGILENGDNIATGELL
                     TEEEYLDILDSLPKDNQQLKDTDPNKFIAKMGAEAIYDLLSRIDLDALSYELRHKANT
                     DTSQQRKAEALKRLQVVEAFRASKNRNRPEWMIVKIVPVIPPELRPLVPLDGGRFATS
                     DLNDLYRRVIIRNNRLKRLIEIKAPEVILRNEKRMLQEAVDSLFDNSRKASAVKTEAN
                     RPLKSLSDSLKGKQGRFRQNLLGKRVDYSARSVIVVGPELKMHECGLPKDMAAELYKP
                     FIIRKLIERGIVKTVKSAKKIIDRKDPVIWDILEYVMKGHPVLLNRAPTLHRLGIQAF
                     QPKLIEGMAIQLHPLACTAFNADFDGDQMAVHLPLSNEAVLEAQLLMLASHNILNPAN
                     GAPITVPSQDMVLGLYYITKPRPNALGEGLIFYSPEEAEIAYNEHKVDIHARVKVRTK
                     DLDENGNSVEKLIETTVGRIIFNKYVPEEVGYINELLSKKSLRDIIGRVIETCGMART
                     AHFLDDIKELGYNMAFRGGLSFNLDDVIIPKDKEKLIEEGNREVEEILNNYNMGFITY
                     NERYNQIIDTWTHVNSKLSDVLMKELSSDNDGFNSVYMMLDSGARGSKEQIRQLSGMR
                     GLMAKPQKSVSEGGQIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKTADAGYL
                     TRRLVDVAQDVTITEEDCGTLRGLIATEMKNNEEVISSLYERILGRVSVHDIYHPLTG
                     ELIVSSGEEITESLAKKIQDSPIEQVEIRSVLTCESKKGVCAKCYGRNLATGRMVNIG
                     EAVGVIAAQSIGEPGTQLTLRTFHVGGIASNIAAESKIVLRYDGRLEFDELRTVQPSD
                     DQSYRVVVSRLAEMRVVDPNTGIVLTTMNIPYGSKLYVKEGDICTKGQLICEWDPFNA
                     VIIAENEGKIEFEDVVENVTFKVETDEATGLREKIIIESKDRNKVPTAHILDKDGNIL
                     KTYNLPVGAHIVFENGDYIKTGQILVKIPRAVGKAGDITGGLPRVTELFEARNPSNPA
                     IVAEIDGEVTFGKIKRGNRELSITSKTGEVKKYLIPLSKQILVQENDFVRAGTPLSDG
                     SITPTDILQIKGPTAVQDYIVNEVQDVYRLQGVKINDKHFEIIVRQMMRKVEIIEPGD
                     TRFLEKQVVDKNDFMEENDRIWGKKVIVDAGDSPTLKPGQIITPRKLRDENSMLKRKD
                     LRPVQARDAIPATSNQILQGITRAALQTNSFMSAASFQETTKVLNEAAINAKVDKLEG
                     LKENVICGHLIPAGTGQRDFEKIVVYSREEYEKKVFARKSLIDVEEGEVVEENN"
     gene            complement(13009..13839)
                     /locus_tag="PLZ54_04355"
     CDS             complement(13009..13839)
                     /locus_tag="PLZ54_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOJ66195.1"
                     /translation="MNSKPDFLIDIEKVVKEKAPNTFIPSFLVSYLRKIVHEKEINEL
                     LTTYSHQKNVDFLESAVKYFQVNVSIKNRENLPHDDKLYIFAGNHPLGGMDAIMTGSL
                     LGREYDGKIRFMANDMLMFIEPLKDLFIPVNKVGSQGKNHVRELNDFFSSDCHLITYP
                     AGKCSRKSNGKIQDEAWNKNFVVKAIQHQRDIVPIYFDGKNSNFFYNLANLRKFFHIK
                     FNIEMLYLSDEMFKQKGKSFSLVIGEPIRWQTFDRSKTYGKWAQWVKEKVYALSEKTF
                     "
     gene            14179..14628
                     /locus_tag="PLZ54_04360"
     CDS             14179..14628
                     /locus_tag="PLZ54_04360"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOJ66196.1"
                     /translation="MSTFIGKYEAKADVKGRIFIPSAYRKILGVDDREQIIMRKDAGN
                     ECIVFYPESIWNQKVENLKSKLDEWNPSDQLLLMQFVADAEWLDIDAQGRVLISKKHL
                     QTIGVENAEVLFVGMIDRFALWSKSRYEQMQLSAENFAALLKEKMSK"
     gene            14643..15563
                     /gene="rsmH"
                     /locus_tag="PLZ54_04365"
     CDS             14643..15563
                     /gene="rsmH"
                     /locus_tag="PLZ54_04365"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOJ66197.1"
                     /translation="MSEKEYHIPVMLKETLQGLVLNPDGIYVDVTFGGGGHSRGILDL
                     LSKKGKLIAFDQDQDACQNIFEDDRFLFVQSNFCYLKNFLRYHGISLVDGILADLGVS
                     SHHFDESERGFSFRLDGELDMRMNRKTELTAATVLNTYTEEQLADVFYHYGELSNARK
                     IAATIVKARDREPFQKILPFVEMMKRFFPRQKEKKGLACVFQALRIEVNHELEALQEF
                     LQQTPDVLKPGGRLAVLTYHSLEDRMVKNFMKTGNFEGILNKDFFGNIIAPFKPISGK
                     AITPQLSEIEKNPRARSAKLRIAELIDKTN"
     gene            15611..15991
                     /locus_tag="PLZ54_04370"
     CDS             15611..15991
                     /locus_tag="PLZ54_04370"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOJ66198.1"
                     /translation="MSWFSKIIENIKGSEDFSELKSSSFRDILNGNIFTKRFFIKQIG
                     LLIMIVVLFIFYIGNRYEYESELTYQNKLKKEVQDKKYESLSISAELMQVSRQSNVEK
                     MLKERGIELHEPVSPLVVLDDSIQ"
     gene            16000..>17772
                     /locus_tag="PLZ54_04375"
     CDS             16000..>17772
                     /locus_tag="PLZ54_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOJ66199.1"
                     /translation="MADQHKNIVFRFGIVYVFIVVAFLLVIYKIITIQFFERENWLAL
                     AAKNVKTNIEVKANRGNIFSSDGRLMASSIPTYYIYMDLRVPALQENNGQLFHENIDS
                     LSLCLSKFFKDKSQAEYKKDITEAYNKKLGAFRIYPKKISYSQLKELKTFPLFRLGKN
                     KSGLITKEQFRRVKPFGSLASRTIGDIYADETKGGKNGLELKFDSLLRGTPGIAIRQK
                     VANTWQETVQVEPIDGIDVITTIDVDIQDIAEKALLDSLRSFDAQEGYVIVMEVQSGE
                     VKAIVNMQKNENGTYSENRNGAVSDMVEPGSTFKVVSLMAALDDGKVKVTDSIDTGNG
                     IFQFANAFMKDHNYNRGGFHKITIAEAIHASSNIGISRMIVNAYGNNPAAYVDKLYKM
                     KVNEPMNLEIPGTAAPNIRHPLDKSKYWSKTTLPWMSIGYETQIPPIYTLAFYNSIAN
                     DGKLIRPYFVKALSKNGQILKTFQTEIINEQICKPSVLQTIRETLLGVVEGRLGTAKN
                     VRSKYVRIAGKTGTAQISKGALGYRSGGKSHQVSFCGYFPAEHPLYTCIVVIREPRIG
                     YPSGGKMSGSVFKDIAERIMFLRST"
BASE COUNT         5629 a         3198 c         3905 g         5040 t
ORIGIN      
        1 gatagagcat tagccttcca agctgagggt cgcgggtttg agtcccgtct tccgctctga
       61 aaatctgaag gaaacaaaca acggttcaca tgaaacagga ccgttgtatt ccttttttta
      121 cagaagggaa ctgtaaaaat tgcacgctgt tgtagctcag aggtagagca cttccttggt
      181 aaggaagagg tcgcgggttc aattcccatc aacagctcta tgtttttctt gaaacaaagc
      241 ttttataaaa cataaattac taattattaa aaaaactatt ctaagattat ggcaaaagaa
      301 aaatttgaca ggtcaaaacc acatgtcaac attggaacaa tcggacatgt tgaccatggt
      361 aaaactactt tgaccgccgc tattaccaaa gtgcttgctg aaaaaggact tgctgaaatg
      421 cgatctttcg attctatcga taatgcacca gaagaaaagg aacgtggaat caccattaat
      481 acttcgcacg tagaatatca aacagctgcc cgccactacg ctcacgttga ctgtccggga
      541 cacactgact acgttaaaaa catggttacg ggtgcagccc agatggatgg agctattatt
      601 gtggttgcag ctactgatgg tccaatgcca cagactcgcg aacacattct tttggctcgt
      661 caggttaacg taccaagatt ggttgtgttt atcaacaaat gcgatttggt tgatgacgaa
      721 gaaatgctcg accttgttga aatggaaatg cgcgaacttc tttcattcta cgatttcgat
      781 ggcgacaata ctccggttat tcgtggttca gctttgggcg cattggatgg tgaccctaaa
      841 tgggaagcta aaattgttga gttgatggat gctgttgaca gctggattga attacctcaa
      901 cgtgatgttg acaaaccatt cttgatgcct gttgaagacg tattctctat tacaggtcgt
      961 ggaactgttg ctacaggcag aattgaaact ggaattgtaa aagttggcga cgaagttcag
     1021 attatcggat tgggacacga agccaagaaa accgttgtaa cgggtgttga aatgttccgt
     1081 aagattttgg atcaaggaca ggccggcgat aatgtaggtt tacttctgag aggtatcgat
     1141 aaggatgaaa tcaaacgagg tatggttatt acccatccgg gagccgttac acctcacaag
     1201 aaattcaaag ctgccgttta tattctgaag aaagaggaag gtggacgtca tactcctttc
     1261 cacaatcact atcgtcctca gttctatatt cgtacaatgg acgttaccgg tgaaattact
     1321 cttccggaag gagtggaaat ggtaatgccc ggcgacaata tcgaatttac tgtagaactg
     1381 atttatccgg tagcttgtag cgtaggtttg cgttttgcta ttcgtgaagg cggacgtacg
     1441 gtaggttccg gtcagattac agaaattttg gactaatttg actgacagat gtcgttcact
     1501 tttttggaca tcactaaaaa ataaacatga attattcttt ttaagctctt ttccttttac
     1561 aggctttcaa atcatcaaag cctgtaaaag gaaaaattac ctacgggcgt agctcagttt
     1621 ggtagagcac tggtctccaa aaccaggtgt cgggagttcg agtctctctg cccgtgccaa
     1681 tttgtacaaa aaacatgaaa ataattaact atatcaaaga gtgctattca gaactggttc
     1741 ataaagtttc ttggccttcg ctctcggaac ttaccaacag cgcaattgtt gtattgattg
     1801 cttccatcat tcttgcgttg attgtgtggg ttatggattt aagttttgaa tctatcatga
     1861 cgttcattta cgaaaaaatc ttttaaactt taatcagtgg aggttgagaa agtgtctgaa
     1921 acgacaaatt ttaaatggta tgtactccgt gccattagtg ggaaagaaaa caaagttaag
     1981 gaatatattg agacagaaat caaaaattct gacctgggcc agtatgtacg tcaggttttg
     2041 atccctactg aaaaagtttt tcagattcgt aacggcaaaa aaattgctaa agaaaggaat
     2101 tgcatgccgg gttacgtttt gatagaagcc aatcttatgg gtgaagctct tcaccgtgtt
     2161 cgcgaaacac ccaatgtttt ggggttttta ggcgaaaaaa acaacattcc cacgcctatc
     2221 cgtacttccg aaattaaccg aattttatgg acaatggatg aattgcagga aagtgaagaa
     2281 gaaatgataa ctcctttcat ggtaggagaa aatgtaaaag tcatcttcgg acctttttcg
     2341 ggatttagtg gaacgattga agaggtaaac accgaaaaga aaaaactcaa agttatggtt
     2401 atgatattcg gtcggaaaac tccgctcgaa ttagcgttta ctcaagtaga aaaggaatag
     2461 tgggctcggt tacaagtcga ttattccgat aacaatcaaa aatcaaaaca attatggcaa
     2521 aagagattgc tggagtaatt aaattgcaga taaaaggagg tgctgcaaat ccatctcctc
     2581 ccgtaggacc tgctttaggt tctaaaggga tcaacattat ggagttctgt aaacaattta
     2641 atgccaggac tcaggacaaa gccggtaaaa tcttacccgt tgtcattacc tattatacgg
     2701 acaaatcttt cgattttgtt gttaaaacgc ctccggtagc cattcaattg ctcgaactta
     2761 ccaaactcaa aagtggatct cccgaaccca accgtaaaaa ggttgccgaa atttcttggg
     2821 atcaggtgaa ggaaattgca caggataaaa tggttgacat gaactgtttc gaattggaat
     2881 cagccatgag aatggtagct gggacagcaa gaagtatggg tattaccgtg aagggtactt
     2941 ttccaggtaa ataacaattt aacacttcaa ttacaatgag tacattaaca aaaaatcgaa
     3001 aatacgcttt agaaaaaatt gaagctggaa aggcatatcc actcattgaa gctgcaaagt
     3061 tagtaaaaga agttactact actaaatttg atgcttcggt ggatatcgat gtgcgtttag
     3121 gtgttgatcc ccgtaaggcc aatcaaatgg tacgtggagt cgtttcactt cccaatggga
     3181 cagggaaaca ggtgaaagta cttgctttgt gccctcctga attggaagct gctgctaaag
     3241 aagcaggcgc cgattacgtt ggactggacg aatacatcga aaaaattaaa ggtggctgga
     3301 cagatgttga tgtcatcatt actacacctg ctgtaatggg gaaattggga gctcttggaa
     3361 gaatacttgg gccccgcggt ttaatgccaa accccaaaag cggtacagta acgaatgatg
     3421 ttgccaaggc tattaaggaa gttaaacagg gaaaaataga tttcaaagtc gacaagtacg
     3481 gaattgttca tgcttccatc ggaaaagttt cctttacggc agaacaaatc gccgaaaatg
     3541 cacaggaatt tatttctacg ctcataaaac taaaaccttc tacagccaaa ggaacttaca
     3601 ttaaaagcat ttatctttct agtacgatga gtccgggtat aaaagtagat cccaaatcaa
     3661 ttgaaattta aagattaaac ttatgaagaa ggaagataaa agcacaatca ttaatcagat
     3721 tgaatcagtt ctgagcgagt atcctcattt ttaccttact gacattggcg gtttaaatgc
     3781 cgctcaaacc agtgagttga gaaaactttg ctacaaggaa aaggtgaaac tggtagtggt
     3841 taaaaatact ttgttgaaaa aagcactgga tcgctcatcg gttgatttca gtgaactgta
     3901 tccaacttta aagggagaaa ctgctctcat gctttccgat atggctaatg ttccggcaaa
     3961 actgattaaa aatttcagtg ccaagaacaa aaaacctgtt ttaaaggctg cttatgttga
     4021 agaaagtttt tacattggcg aaaatcaact cgatacactt gtcaatgtga agagcaagaa
     4081 tgaactgatt gccgatttga ttgctttgtt gcaatctccg gctaaaaatg tcgtttcagc
     4141 tctacaatca ggagctaata ctattcatgg tgttttacaa acactttctg aaaaataatt
     4201 tttttaaatt aaaaacaaat caaaaaatag aataaaatgg cagatttgaa agcttttgca
     4261 gaacaattgg ttaacttgac agtaaaagaa gtgaacgagt tagcacagat tttaaaaaat
     4321 gattatggta tcgaacccgc tgcagcagct gttgctgttg ctgcaggccc gtcagctgca
     4381 gcagcagagg cagttgaaga aaaaacagct tttgatgtag tattgaaatc gccgggtgcc
     4441 aacaaattgg caattgtaaa attagtaaaa gaacttaccg gtttgggact taaagatgcc
     4501 aaagatttag ttgacgctgc tccttctgtt ataaaggaag gaatttcgaa agatgaagct
     4561 gaagccttaa aaaatcagtt ggttgaaggc ggagctgaag tcgaaattaa ataatgtcag
     4621 ctgaatatca gtaacttggt ttagaacctt tcgcaacggg aggttctaaa cctttttgtc
     4681 tatacaatat agtgagttca ttgaacccca ataacctctt tgaaaatgtc ttcatctact
     4741 aacaatcaaa gaataaattt tgcttccata aaaaatcagt tggagtatcc tgattttctg
     4801 gaagtacaag tgaaatcttt tcaggatttt ttccaaatgg atacttcctc cgacaaacga
     4861 aaatcggaag gtttatacaa agtgtttacc gagaatttcc ccatcgctga tactcgaaat
     4921 aattttgtac tcgaatttat agattattac atagatccac cgcgctattc tatcgaagaa
     4981 tgcattgagc gaggtttaac ttacagtgtg cctttaaagg caaaacttaa actttattgc
     5041 accgatcctg aacatgagga tttcgatacc gttattcagg atgttttctt gggtccaata
     5101 ccttatatga ctcccagagg tacttttgtt atcaatggag ccgaaagggt tattgtttct
     5161 caattacacc gttccccggg ggtatttttt ggacaaagta ttcatgccaa cggaacaaaa
     5221 ttgtattcag ccagaattat tccatttcgt ggctcgtgga tagaattcgc taccgacatc
     5281 aacaatgtca tgtatgctta tattgacaga aagaaaaaat tgcccgtaac cacgttgctc
     5341 agagccattg ggtttgaaag tgataaggat attttggaaa tttttaatct ggctgaagaa
     5401 atcaaagtca ataaatccaa tctgaaaaag gctgtgggca gtaaacttgc tgcccgtgtg
     5461 ctgaaaacat ggttggaaga tttcgtggat gaagataccg gtgaagtagt aaccattgaa
     5521 cgtaatgaaa tcattatcga tcgtgaaacc gttttagaaa acgagcacat tgatctgatt
     5581 ctccaatccg gggctcaaac catactgata cataaagaag aagccaatca gaacgattac
     5641 agcattattt acaatacgct gcaaaaggat ccaagcaact cggaaaaaga agctgtactt
     5701 tatatttaca ggcaattgag aaatgctgaa ccaccagatg atgctactgc ccgtgaagta
     5761 atcaacaatc tgttcttttc ggaaaaaaga tatgatttgg gtgaagttgg acgtttccgt
     5821 atcaatcaaa agctgaacct cactacaagt atggaggtga aagtgctcac caaagaagat
     5881 attattgaaa ttataaaata tctgatcgat ttggtgaact ctaaggctga agtggacgat
     5941 atcgatcacc tgagcaacag aagagtcaga acagttggag aacaattata caatcagttt
     6001 agcgttggat tggcacgtat ggcacgtacc attcgggaac gaatgaatgt tcgcgataat
     6061 gaagttttta ctcccggcga tctgatcaat gccaagagta tttcatcggt tattaattca
     6121 ttctttggaa ccaatgcttt atcgcaattc atggatcagc agaatccgct tgctgaaatt
     6181 acccacaagc gaaggatgtc ggctttgggg cccggcggtt tgtctcgcga aagagccggt
     6241 tttgaagtac gtgacgttca ttatactcat tatggtcgac tgtgtcctat agaaacacct
     6301 gaaggtccaa atatcggtct tatttcgtct ttgtgtgtat atgcaaaaat caacgaactc
     6361 ggttttatag aaactcctta ccgtaaagtt aataatggag tagtcgattt gagtgaaaac
     6421 ggaattggtt actatactgc tgaagaagaa gaagatttgg ttgtagcaca agggaatgct
     6481 cctcttgatg aaaacggaaa atttattcgt tcgagagtga aagcacgtct tggcgctgat
     6541 tttccggtag ttgacccaat ggaagttcat ttgatggatg tttctccttc gcaaattgcg
     6601 tctattgcag ccggattaat tccttttctg gaacatgatg atgccaatcg tgccttaatg
     6661 ggttcgaaca tgatgaggca ggcagttccg ttgctacggt gtgaagcccc gattgtggcc
     6721 accggcatag aagggagagt gattcatgat tcgcgcacgc aaataactgc tgagggagat
     6781 ggtgtagttg aatatgttga tgcaaattgc attaaaattc gctatgaccg cactgaagaa
     6841 gaagaatttg taagtttcga ttctgctgtt aaggaatata aacttcctaa atatcaaaag
     6901 acaaatcaga ataccaccat tgaccttcgt ccaattgttc gcaggggaga ccgtgtacac
     6961 gcaggaatga ttatcaccga aggctatggc agcgaaagtg gagagttggc tttgggcaaa
     7021 aacttgaaag ttgcttttat gccgtggaaa ggatacaatt tcgaggatgc tattgtgata
     7081 aacgaaaagg tggttcgaga agatgtcttt acttccattc acgtagtgga acaattgttg
     7141 gacgttagag aaaccaagcg tggcactgag gaatttactt ccgacatacc caatgtgagt
     7201 gaagatgcta ccagaaatct tgacgaaaac gggattattc gagttggtgc acgcgtagaa
     7261 cccggtgata taattatcgg aaaaataaca ccaaaaggag aatccgatcc ttctcccgaa
     7321 gaaaagttgc ttcgtgcaat attcggcgat aaggcaggcg atgtaaaaga tgcttctctg
     7381 aaagccacac catcgttgtc gggggtagtc attggaaaaa gattattctc caaagctcag
     7441 aaaaatcgta aatcaaaatt agctgacaag gcagttcttc caaaattgga cgaagagttt
     7501 gaaacacaag ccaatgcgtt gcgtgaaact ttgatagaaa agttgatgaa aattttgacc
     7561 aacaagactt caaccggtgt gaaggacttt ttgggtaccg atattatacc acgtaacagt
     7621 aaatttactg ttgagcggtt gcgcgaaata gattatacta cggttcagct tggaaaatgg
     7681 acaaacgacg ctcatacaaa tgacatggtg aacagtttga ttaccaattt cttaagaaaa
     7741 ttcaaagaat tggatgcgca gctgaaaagg caaaaattca acattaccat tggagatgat
     7801 ttgccaagtg gcattatgca aatggccaaa gtttatatag ccaaaaaacg taaaattcgt
     7861 gttggggata aaatggcagg tcgtcacggg aataaaggta ttgtttcgcg gattgtacgc
     7921 gaagaagaca tgcctttcct cgaagatgga acgcctgttg atattgtgct caacccgttg
     7981 ggtgtacctt cgcgtatgaa tttgggtcag atttttgaaa gtgttttggg atgggcaggt
     8041 aaagaattgg gagttcgttt tgcaacgcca atttttgatg gtgccacgct caacgactta
     8101 aatgcttgga cagacaaagc agggttgccc cgtgcaggta aaacttattt gtacgatggc
     8161 ggaaccggcg aacgcttcga ccaacctgcc acagtaggcg ttatttacat gatgaaactg
     8221 ggacacatgg tggaagataa gatgcacgct cgttctattg gaccttattc tctgattacg
     8281 cagcaacctt tgggaggaaa agcccagttc ggcggacagc gatttggaga aatggaagta
     8341 tgggcactcg aagcattcgg ggctgccaat atcctgcgtg aaattcttac cgttaagtcg
     8401 gatgatgtca atggtcgtgc ccggacttat gaagcaatcg tgaagggaga tccaatgcct
     8461 actcccggaa tcccggaatc attgaatgta ttacttcacg aattacgcgg tctgtgcttg
     8521 agcattaatt tggaataggg aacgtgaatt tatcataacg ttcattccta tccaaaatta
     8581 atttaaaatt gtcaatttaa ttaaattgaa ataaaaaata tggcttttaa aactgataat
     8641 aaaataaaga gcaatttctc gaaaatttct atcggattag cttcacccga agaaattttg
     8701 aaattgtctc atggtgaagt actcaaaccc gaaacaatca actacagaac ttacaagcct
     8761 gaaagagacg gtttgttttg cgaacgcatt tttggccctg taaaggacta tgaatgtcat
     8821 tgcggaaaat acaagcgtat tcgatacaag ggaattgttt gtgatcgttg cggggtagaa
     8881 gttacagaga aaaaagttcg tcgtgaaaga atgggacata tccaactggt tgttcctgtt
     8941 gcccatatat ggtatttccg cactttaccg agcaaaattg gctatctgct gggaatgtcg
     9001 agcaaaaaac tggaatcgat tatttattac gaaaaatatg tgattattca gggaggcatt
     9061 cttgaaaatg gagacaatat tgccaccgga gaactactca ccgaagagga atatttggat
     9121 attttggatt cgctgccaaa agataatcaa caactgaaag acaccgatcc caataagttt
     9181 attgctaaaa tgggtgcaga agccatttac gatctgttga gcagaataga tttggatgcg
     9241 ctttcctatg aattgcgtca taaggccaac accgatactt ctcagcagcg taaagccgaa
     9301 gctttgaaac gtttgcaggt ggtggaagct ttcagagctt ctaaaaatag aaatcgtccc
     9361 gagtggatga ttgtgaagat tgttccggtt attcctcctg aattgcgtcc gttggttccg
     9421 ttggatggcg gtcgttttgc cacttcagac ttaaatgatc tttatcgtcg agtaattatt
     9481 cgcaataatc gcctgaaacg tctgattgaa atcaaggcac cggaagtaat tttacgcaat
     9541 gaaaaacgta tgttgcagga agctgtggat tcgttgttcg acaattcgcg taaagcaagt
     9601 gctgtgaaga ccgaagccaa ccgtccttta aaatcacttt ccgattcatt gaaagggaaa
     9661 cagggacgtt ttcgtcagaa cttgctggga aaacgtgtgg actattcggc tcgttcagtt
     9721 attgtggtag gcccggaatt gaaaatgcat gaatgtggat tgcccaaaga tatggctgcc
     9781 gagctttaca aaccgtttat tatccgaaaa cttatcgaaa ggggaatagt aaaaactgta
     9841 aaatcggcaa aaaaaattat tgatcgcaag gaccctgtta tctgggatat tctggaatat
     9901 gtaatgaaag gacatccggt tttacttaac cgagctccaa cattgcaccg actgggaatt
     9961 caggcattcc aacccaaact tatcgaaggg atggccattc aattgcatcc tttagcttgt
    10021 acggctttca atgccgactt tgatggagac caaatggccg tgcatttacc gttgagcaat
    10081 gaggctgttt tggaagctca attgttgatg ctggcttctc acaatatttt gaatccggcc
    10141 aacggagctc ctattactgt tccttcacag gatatggtat tgggattgta ttatattaca
    10201 aaacctcgac caaatgcttt gggtgaaggt ttgatatttt attctcccga agaagcagaa
    10261 attgcttata atgaacataa agtagatatt catgctcgcg taaaagttcg taccaaagat
    10321 ttggacgaaa acgggaattc ggtggaaaaa ctgattgaaa ccactgttgg acgaattatt
    10381 ttcaataaat atgttccgga agaagttggc tatatcaacg aattgctttc aaaaaaatcc
    10441 ttgagagaca ttatcggcag ggttatagaa acttgtggaa tggcgcgtac ggctcatttt
    10501 ttggacgata tcaaggaatt gggctacaat atggccttta gaggcggact ttccttcaat
    10561 ttggatgatg tgattattcc aaaagataag gaaaaactta ttgaagaagg caacagggaa
    10621 gtagaagaaa ttctgaataa ctacaatatg ggattcataa cctataacga acgttataat
    10681 cagattatcg acacttggac gcacgtaaat tccaaattgt ccgatgtgct gatgaaggaa
    10741 ttgtcgagcg ataatgatgg attcaattct gtgtatatga tgcttgattc gggagcgcgc
    10801 ggttcaaagg aacagattcg tcagttgtcg ggaatgcgtg gtttgatggc aaaacctcag
    10861 aaatcggtat cggaaggtgg acaaattatt gagaacccga ttctttccaa ctttaaggaa
    10921 ggactttcgg tgttggaata ctttatctcc acgcatggtg cccgtaaggg tttggccgat
    10981 acggcactta aaacggctga tgcagggtat ctgactcgac gtctggtcga tgttgctcaa
    11041 gatgtaacca ttactgagga agattgcgga acattgaggg gcttgattgc taccgaaatg
    11101 aaaaataatg aagaagtaat ttcttctttg tatgaaagaa ttttgggacg tgtttccgtt
    11161 cacgatattt atcatccgct taccggagaa ttaatcgtat cttcgggaga agaaattaca
    11221 gaatcactgg ccaaaaaaat tcaggattct cctatcgaac aagttgaaat tcgttccgta
    11281 ttgacctgcg aatccaaaaa aggagtctgt gcaaaatgtt acggacgaaa tttggcaaca
    11341 ggaagaatgg taaacattgg agaagctgta ggtgtcattg ctgcccagtc aattggagag
    11401 ccgggaactc agctgacact tcgtaccttc catgttggag gtattgcttc aaatatagct
    11461 gccgaatcaa aaattgtact tcgttatgat ggacgtctcg aatttgacga acttcgtacc
    11521 gttcagccct ctgatgatca aagctatcgc gttgtagtaa gtcgtttggc cgaaatgcgc
    11581 gttgtggatc ctaatacggg tattgtgctt actaccatga atatccctta tggatcgaag
    11641 ttgtatgtga aggaaggaga tatttgtact aaaggtcagc ttatttgcga atgggatcct
    11701 tttaatgcgg ttataattgc agaaaacgaa ggaaaaatcg aatttgaaga tgtggtcgaa
    11761 aacgttactt ttaaggttga aaccgatgaa gctacgggac ttcgagagaa aattatcatc
    11821 gaatcgaaag accgaaacaa agttcctaca gctcatattc tggataagga tggcaatatc
    11881 ttgaaaactt ataatttacc ggttggagct cacattgtgt ttgaaaacgg tgattatata
    11941 aaaaccggtc agattctggt aaaaattcct cgtgctgttg gaaaagccgg agatattacc
    12001 ggaggtcttc cacgagttac ggaattgttt gaggctcgaa atccttctaa tcctgccatt
    12061 gtggctgaaa ttgatggaga agttactttc ggaaaaatca aacgtggaaa tcgtgagtta
    12121 agcatcactt ccaaaaccgg tgaagttaaa aaatatctga ttcctctttc aaaacaaatt
    12181 ctggtacagg aaaacgattt tgttcgtgca ggtacgcctc tctctgatgg ctcgataacg
    12241 ccaaccgata ttttgcagat taagggacct actgccgttc aggattacat tgtcaacgaa
    12301 gttcaggatg tttaccgcct gcaaggggtg aaaattaacg acaaacattt tgaaattatt
    12361 gttcgtcaga tgatgcgcaa ggttgaaatc attgaaccgg gagatacccg tttcctcgaa
    12421 aaacaggtag ttgacaaaaa tgattttatg gaagaaaacg acagaatttg gggaaagaaa
    12481 gttattgtgg atgcgggaga ttctcctact ttgaaacccg gccaaattat tacgcccaga
    12541 aagttgaggg atgaaaacag tatgttgaaa cggaaagact tgcgtccggt tcaggctcgt
    12601 gatgccattc cggcaacatc caatcagatt ttgcagggaa ttactcgtgc tgcacttcaa
    12661 accaacagct ttatgtcggc tgcttctttc caagaaacaa caaaagtgct gaacgaagct
    12721 gccattaatg ccaaagtgga caagctggaa ggcttgaaag aaaacgttat ttgcggtcat
    12781 ttaatccctg caggtactgg acagcgcgac tttgaaaaaa ttgttgtcta ttcccgtgaa
    12841 gaatacgaaa agaaagtttt tgcacgtaaa agcttaattg atgttgaaga aggtgaagta
    12901 gtagaagaaa acaattaaaa cttatatcta ttttttcaaa aggcagggct ttttttgctc
    12961 tgtctttttt ttattaatgc ccttatttta ggaataatcg taattttatt agaaagtttt
    13021 ttcggacaag gcataaactt tctctttgac ccattgcgcc catttgccgt aagtttttga
    13081 acggtcaaaa gtttgccaac gaataggctc gccgataacc agcgaaaaac ttttcccttt
    13141 ttgtttgaac atttcgtcgg acagatagag catttcgata ttgaacttta tatgaaaaaa
    13201 ttttctcaga ttggccaggt tgtaaaagaa atttgaattt tttccgtcaa aataaatcgg
    13261 gacaatatcc cgctgatgtt gaatagcttt gacaacaaaa ttcttgttcc atgcttcatc
    13321 ctgaattttt ccgttggatt ttctgctgca ttttccggcc ggataagtaa tcagatgaca
    13381 atcggaactg aaaaaatcgt tcaattcgcg aacatggttt ttcccctgtg aaccgacttt
    13441 gttgaccggg ataaacaagt ccttcaaggg ctcgataaac atcaacatat cgttggccat
    13501 aaatcgaatt ttcccatcat attccctgcc caacaagcta ccggtcatta tggcatccat
    13561 tcctcccaac ggatgatttc cggcaaaaat ataaagttta tcgtcatgag gcaaattttc
    13621 tctgttttta atggaaacat tgacctgaaa atattttact gcgctttcga gaaaatctac
    13681 attcttttgg tgcgaataag tggtcagcaa ctcattgatt tccttttcgt gaacaatttt
    13741 acgcaaataa ctcaccaaaa aagaagggat aaaagtatta ggcgcttttt ccttgacaac
    13801 tttttctata tcaattaaaa aatccggttt actattcatg aaaataaaat ggtgttgttc
    13861 tattgcaaag ttacaataat ttgaaatttt tgtgtttttt atcattgcat ttttcgttga
    13921 aaagttatca acatttttga aattcacaaa tatttttcac cacttaccat ataaaaatga
    13981 atgaataaaa tataaaatct ttattttctg atgtttacaa aataattgtc taatttcttg
    14041 ttgttgataa attgttgaaa agcggttgaa aacaagctaa aataatttaa aaaaattgtt
    14101 tataactttt ttgtgggtga atgtggggaa ttgaaaataa ttccatattt ttacagttga
    14161 aaaaatatag ctactgcaat gtctacattt attggaaaat atgaagccaa agccgacgta
    14221 aaaggtcgca tattcattcc ttccgcttat cgaaaaatct tgggcgtaga cgaccgtgaa
    14281 caaatcatca tgcgcaaaga cgcagggaat gaatgtattg tgttctatcc ggaaagtatc
    14341 tggaatcaaa aggtagaaaa cttaaaatcg aaattggacg aatggaatcc ttccgatcag
    14401 cttttactga tgcagtttgt ggcagatgcg gaatggctgg atattgatgc gcagggacgt
    14461 gtattgattt cgaaaaaaca tttgcagacc attggcgttg aaaacgcaga agtgcttttt
    14521 gtgggcatga ttgaccgttt tgcgctttgg agcaaaagtc ggtacgaaca aatgcaattg
    14581 tcagccgaaa attttgcggc tttgctgaag gaaaaaatgt caaaataact aattataatt
    14641 tcatgtctga aaaggaatat cacatacccg tcatgctgaa ggaaacgctg caaggattgg
    14701 tactcaatcc ggacggtatc tatgtggatg taaccttcgg cggcggagga cattcacgtg
    14761 gtattctcga tttactgagc aaaaagggaa aacttatcgc ttttgatcag gaccaggacg
    14821 cctgccaaaa tatttttgag gatgacagat ttttgttcgt tcaaagtaat ttttgttatt
    14881 taaaaaactt tctacgttat catggtattt ctctggtaga tggcatttta gccgatttgg
    14941 gcgtttcttc gcatcacttt gatgagagcg aacgggggtt ttcgttccgg cttgatggag
    15001 aattggatat gcgcatgaac agaaaaacgg aactgacggc tgctaccgtg ctgaacacct
    15061 acactgaaga acagttggcc gatgtttttt atcattacgg agaattgtcc aatgcacgaa
    15121 aaattgcagc aaccatagta aaagcgagag acagggaacc ttttcaaaaa attcttccgt
    15181 ttgtagaaat gatgaaacgc tttttccccc gtcagaaaga aaaaaaagga cttgcctgcg
    15241 tttttcaggc tttgcgcatt gaagtaaatc acgaattgga agctttgcag gaatttctgc
    15301 agcaaacccc cgatgtgctg aaacccggag gaagattggc cgtgctcact tatcattcgc
    15361 tcgaagaccg catggtgaaa aattttatga aaacagggaa ctttgaaggt attttaaaca
    15421 aagatttttt tgggaatatc attgctccct tcaagcctat aagcggcaag gctattacgc
    15481 cacagctttc ggaaattgaa aaaaatccga gagccagaag cgccaaatta cgcattgccg
    15541 aattgattga caaaactaat tgattgattt tttgaacatt taatgaaaga gttaaacttt
    15601 gaaataacga atgagttggt tcagtaaaat catagagaac ataaagggca gtgaggattt
    15661 ttccgaactc aagtcgagct catttcggga tatcctcaac ggaaatattt tcaccaaaag
    15721 attttttatc aagcaaatag gtcttttgat tatgattgtg gtgcttttta tattttatat
    15781 tggcaaccgc tacgaatacg aatcggaatt gacctaccaa aacaaattga agaaggaagt
    15841 ccaggacaaa aaatatgaat cgcttagcat ctcggcagaa ctgatgcaag tcagccgtca
    15901 gtcgaacgtg gaaaaaatgt tgaaagaaag aggcatcgaa ctgcacgagc cggtttctcc
    15961 tttggtagtg ttggacgatt cgatacaata agaattgata tggcagacca acataaaaat
    16021 attgtatttc gctttggcat tgtatatgtt tttatagtcg ttgcctttct gctggtgatt
    16081 tacaaaataa ttaccataca gttttttgag cgcgaaaatt ggctggcttt ggcagcaaaa
    16141 aatgtaaaaa ccaatataga agtgaaagcc aacagaggaa atattttttc ttccgacgga
    16201 cgtttgatgg ccagttccat acctacttat tacatttaca tggacttacg ggtgccggca
    16261 ttgcaagaaa acaacggaca actgtttcac gaaaatattg actccttgtc gctttgtttg
    16321 tcaaaatttt ttaaagataa gtctcaagcc gaatacaaaa aagacattac ggaagcttac
    16381 aacaaaaagt tgggagcttt tcgcatttat ccgaaaaaaa tctcttatag tcaattaaag
    16441 gaattaaaaa cttttccgtt gtttcgtttg ggaaaaaaca aaagcggttt gataacaaaa
    16501 gaacaattta gaagagtaaa acctttcggg tcgctggcct cccgaaccat tggagatatt
    16561 tatgctgacg aaaccaaagg cggaaaaaac ggtcttgaac ttaaattcga ctccctttta
    16621 agaggaacac ccggaatagc cattcgccag aaagttgcca atacatggca ggaaacggta
    16681 caagtggaac ccattgacgg gattgatgtg attaccacta ttgatgttga cattcaggat
    16741 attgccgaaa aagcgctgtt ggacagttta aggtctttcg atgcgcagga aggttacgtt
    16801 attgtaatgg aagttcaatc gggagaagtt aaagccattg taaacatgca gaaaaatgaa
    16861 aatggaacct attccgaaaa tcgaaacggc gctgtttccg atatggtgga gccgggctct
    16921 acgtttaaag tcgtttctct gatggctgca ctcgacgacg gtaaagttaa agttaccgat
    16981 tcaatagata ccggaaacgg aatttttcag tttgctaatg cttttatgaa agatcacaat
    17041 tacaacagag gcggttttca taaaatcacc atagccgaag caattcatgc ttcttctaac
    17101 atcggaattt cccgaatgat tgtaaatgct tacggcaaca atccggctgc gtatgtagat
    17161 aaactgtaca aaatgaaagt gaatgaacca atgaatttgg aaattcccgg aacagccgct
    17221 ccaaatatcc gtcatccgct ggataaatca aaatattggt cgaaaaccac tttaccttgg
    17281 atgtcgatag gatatgaaac ccaaattcca cccatatata ctttggcttt ttacaattca
    17341 attgccaatg atggaaaact gatacggcct tattttgtaa aagcgctgag taaaaacgga
    17401 caaattttaa aaacctttca gaccgaaatc atcaatgaac aaatttgcaa gccatctgtt
    17461 ttgcaaacca ttcgagaaac gttgttgggg gttgtagaag gccgattggg aacggctaaa
    17521 aacgtacgtt ccaaatacgt tcgcattgca ggaaaaacgg gaacggcaca aatttcaaaa
    17581 ggtgcgctgg gttatcgttc gggaggaaag tcacaccaag tgtctttctg cggatatttt
    17641 ccggccgaac atccactcta tacttgcatt gtcgtgattc gtgaaccgag aatcggctat
    17701 ccttcgggcg gcaaaatgag tggttcggta ttcaaagata tcgccgaacg aatcatgttt
    17761 ttgagatcaa ca
//
LOCUS       DAOSCH010000037        17686 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2216_length_17686_cov_6.305598, whole genome shotgun sequence.
ACCESSION   DAOSCH010000037 DAOSCH010000000
VERSION     DAOSCH010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17686
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2216_length_17686_cov_6.305598"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1468)
                     /locus_tag="PLZ54_04380"
     CDS             complement(<1..1468)
                     /locus_tag="PLZ54_04380"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOJ66200.1"
                     /translation="MDFFTQDIQFIPGVGPKRAELFKKELNIRTAEDLLRHYPYKYVD
                     RSRFYMISEISENMPFIQLKGKILKFEKVGEGCSQRLIGVFTDGRQYIELVWFKGLKF
                     VAERYKTNIEYVVFGKPTLFNGKYNIVHPDIEILSQLPPVEQMGLQPFYNTSEKMKTH
                     FLNSKAIQKIIYSLLPTLQNGIPETLPAYILNRYGLLNLKDALVNIHFPKNSHLLNRA
                     RQRLKFEELFYIQLDIVKQTAWRNQRFKGFVFKHVGYYFNTFYRDYLPFELTNAQKKV
                     IREIRSDVASGKQMNRLLQGDVGSGKTLVALFAMLMAVDNGFQACMMAPTEILAHQHF
                     ESLKQFLGNIGIQIALLTGSTRPKERAAIHEKLLSGELNILLGTHALIEETVQFKNLG
                     LVVIDEQHRFGVAQRARLWSKNVQPPHILVMTATPIPRTLAMTIYGDLTVSVIDELPP
                     GRKPVQTFHFYDNKRQALYKFMVKQIEAGRQIYVVYPLI"
     gene            complement(1501..2235)
                     /locus_tag="PLZ54_04385"
     CDS             complement(1501..2235)
                     /locus_tag="PLZ54_04385"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOJ66201.1"
                     /translation="MELTKIVLIVAGGKGERMQSDIPKQFIEIAGKPILMHTIEAFAR
                     YDFDLPVFVVLPVTQIEFWKNLCSKYSFKFSHQVVIGGETRFHSVKNGLEAVAAFLAS
                     LYPDKSEEENNSALVAVHDGVRPLVSVETIRRCFDEAEKFGSAIPSMDLVDSLRTIHT
                     KYSQSLDRSAYKLVQTPQVFDFNILKKAYEQEFSPLFTDDASVVEAIGVNVHLTEGNR
                     ENIKLTTAFDLELASFLLAKRENSSY"
     gene            2390..2866
                     /locus_tag="PLZ54_04390"
     CDS             2390..2866
                     /locus_tag="PLZ54_04390"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOJ66202.1"
                     /translation="MMKNFKFLLFTIFLAVSGGLQAQDESTSVRQNIFEVLQASDGAG
                     KVIIHQDKRIENLINQKQTAKTGREATLGNGYRVQVFSSNEQRTGKVEAFKIEKEIRE
                     AFPNTGVYVNYNSPFWKVRVGDFSSVDEAQAFRNKLLEVFPDLRSQTYVVRDQIVK"
     gene            2890..3483
                     /gene="folE"
                     /locus_tag="PLZ54_04395"
     CDS             2890..3483
                     /gene="folE"
                     /locus_tag="PLZ54_04395"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOJ66203.1"
                     /translation="MDFDITSSRPFDEKKTGEIAQHYQEIIRLLGENPDRQGLLQTPQ
                     RVARAMQFFLQGYEQDAEEIIRTATFTENYRQMVIVKDIDFYSLCEHHLLPFFGKAHV
                     AYIPNKKITGLSKIARVVEIYARRLQVQERMTTQIKQCIQNTLDPMGVMVIIEAQHLC
                     MQMRGIQKQNSITTTSDFTGVFQEARTREEFMNLINR"
     gene            complement(3784..6333)
                     /locus_tag="PLZ54_04400"
     CDS             complement(3784..6333)
                     /locus_tag="PLZ54_04400"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOJ66204.1"
                     /translation="MKPKTYTAEEVKKASFEYFQGDELAATVWSTKYALKDSYGNFYE
                     LTPDDMHKRLAREIARIEKHYPNPLTEDELFELFRHFKYIVPQGSPMTGIGNDFQIAS
                     LSNCFVIGFDHDADSYGGIIKIDEEQVQLMKRRGGVGHDLSHIRPKGSPVKNSALTST
                     GIVPFMERYSNSTREVAQDGRRGALMLTVSIKHPDSESFIDAKLEAGKITGANISVKI
                     HDDFMQAALAQETYTQQFPIEDDHPWFKKEIDAHSLWKKIIHNAWQSAEPGVLFWDTI
                     TRESVPDCYADLGFKTISTNPCGEIPLCGYDSCRLLAINLYSYVNHPFTPEASFDFDL
                     FKKHVAVAQRIMDDIIDLEMEKIDKIMAKINADPEDEEIKRTELKLWEKIKNKTSMGR
                     RTGVGTTAEGDMLAALGLRYGTNSAIDFSEQVHKILALAAYRSSVTLAKERGAFSIYD
                     AKREEKNPYILRLREADPELYDEMVKHGRRNIACLTIAPTGTTSIMTQTTSGIEPVFM
                     PVYTRRRKVNPNDKNVRVDFVDENGDSWEEYVVFHHKFVTWMEANHYSTTQKYTKAEL
                     DELVEKSPYYKATANDIDWLKKVQMQGRIQKWVDHSISVTINLPADTTEELVGQLYEE
                     AWRCGCKGVTVYREGSRAGVLLSVEEKKSEKSGERKICFPQSEEHLIRPKELDCDVVR
                     FQNAKDKWIAFVGLKDGRPYEIFTGLADDEEGIALPKTVTQGKIIKTVDENGNKRYDF
                     QFTNKRGYKTTIEGLSHKFDKEFWNYAKLISGVLRYGMPIDQVIKLVSGLQLDSESIN
                     TWKVGVERALKKYIPDGTEANGKNCPSCGQKTLIYQEGCLTCKNCGYSHCG"
     gene            complement(6633..7724)
                     /locus_tag="PLZ54_04405"
     CDS             complement(6633..7724)
                     /locus_tag="PLZ54_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HOJ66205.1"
                     /translation="MYIESINIVNYKNIRQAELSFSPKINCFIGNNGMGKTNLLDAVY
                     FLSFCKSHSNFIDSQNIFHEADFAVLQAKYRMGEATEEIYCGMKRNQKKQFKRNKKDY
                     ERLSDHIGLLPLVLISPEDSVLISEGSDERRKFVDGVISQYDKKYLHNLLLYNNALKQ
                     RNALLKVETPVDESLFEILEEQMADYGSYIFEQRTRFIDQLIPVFQQYYRYISSHENE
                     EIDITYLSHFQQNDIKKQMKSTRERDKMLGFSTRGIHKDELEMLLNGYPIKRVGSQGQ
                     NKTFLVSLKLAQFKFLKQTHHQPPILLLDDIFDKLDADRVRKIVELVSQETFGQIFIT
                     DTNREHLDQILYQLDQEAKIFKVENGIIL"
     gene            complement(8268..10565)
                     /locus_tag="PLZ54_04410"
     CDS             complement(8268..10565)
                     /locus_tag="PLZ54_04410"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOJ66206.1"
                     /translation="MTKLTKEDALLYHSQGKPGKIEVVPTKPHSSQRDLSLAYSPGVA
                     EPCLEIEKDPATVYDYTNKGNLVAVISNGTAVLGLGDIGAMAGKPVMEGKALLFKIFA
                     GIDVFDIEVNEKDPEKFIQAVKAISPTFGGINLEDIKAPECFMIEERLKAELDIPIMH
                     DDQHGTAIISGAGLINALEIAGKRMEDVKIVVNGAGAAAVSCTKLYIRLGAKPENIVM
                     LDSKGVINKQRTDLNETKKAFATDREINTLEEAVKGADVFLGLSVAGVLSIEMVRSMN
                     ENPIVFALANPNPEISYEDAISAREDIIFATGRSDHPNQINNVLGFPYIFRGALDVRA
                     TCINEEMKLAAVYALAELAKKPVPDVVNAAYNLKRLSFGRDYIIPKPLDPRLLTIVAP
                     AVARAAMESGVARKHITDWAGYNAKLRETMGYDDKMLRSFYETARQNPKRVVFSEANH
                     LNMLKAAEMAYNEGVCFPVLLGNEEKINSIAREYNIDLSGIEIINLRHDREEARRIAY
                     AEKLAKKLGREGMTYDEAYEKMYERNYFGMMMVETGEADALITGVYTKYADAIQAAKD
                     VIGIRPGSKHIAAMHIMNTKKGTFFMADTLFNRHPSTETLIDIAKLTYRTVKFFAHEP
                     VMAMLSYSNFGSDKQGSPASVHEVVETLHREVPEMIVDGEMQVNFALNKELRDRKYPF
                     TRLAGKDVNTLIFPNLSSANSAYKLLLEMGISEAVGPIQMGLNKPVHILDVESSVRDI
                     VNMTAIAVLDAIVEEKITTENLERI"
     gene            10992..12941
                     /locus_tag="PLZ54_04415"
     CDS             10992..12941
                     /locus_tag="PLZ54_04415"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOJ66207.1"
                     /translation="MKEIFKFLNNTDTFSHLASLKYLPRWVVLFIDITISAISFLAAY
                     LVASSFIKHLPDQLLLPLFPRLILLLFTQSVFFWLFHTYSGILRYSTMTDAAKLFLAI
                     FGNCIFLSALNFVCSLYFPAPLFYYASILIYGVISFILLFGLRLTVRNIFNYFVETSA
                     QIIPVMIYGIDEAGLGIARMILSSVNSKKQYKLVGFIDGNENIVNKMVMGLRVYKHDD
                     HTIKTKVANKVNTVIVSPIKMKEINPDVDLDIFIQHHISLLTSQPMDIWEGEMPSVRQ
                     IKPLKIEDLLEREPIQISFDNIAAELKSKTVMVTGAAGSIGSEIVRQLCNFTPKLIVL
                     VDNGETAIHNLKIELQAFSTSQHFNFVLGDVRNKERMEYIMDLYRPDFVFHAAAYKHV
                     PMMEDFPCESVQVNVLGTKIMADLALKYRVSRFVMVSTDKAVNPTNVMGASKRIAEIY
                     VQSLARKMQKTGTETTKFITTRFGNVLGSNGSVVPFFQKQIEKGGPVTVTHPDIIRYF
                     MTVPEASSLVLEAGVMGEGGEIYVFDMGNPVNISHLAKKMIQMAGLIPGVDIQIAYTG
                     LRPGEKLYEELLSHKEKVRSTQHPKIMVASVAEYDFDKISVDIDNLIKYSYLCKDFLV
                     VSMMKQIVPEFKSNNSQYELLDISN"
     gene            13078..14157
                     /locus_tag="PLZ54_04420"
     CDS             13078..14157
                     /locus_tag="PLZ54_04420"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOJ66208.1"
                     /translation="MNYLYNFIQQNPYLLILFSFLLGKAFMPSVVRIAKKHNFVVKPN
                     KRTTHKGLIPYTGGFDIFISFIFTILIFCFPLMEKLQYIPIGIFIILMTGFIDDLLNI
                     KPTSKLLGEFVAGVFLIVFSDIRITNFFGVFGIHELPLSISYIFSFLVFLLIINSINL
                     IDGVDGLASGLGIFYNLFFGIYFHLTGNLSLAMIAYTLTGSLAVFFIYNVFSKKNKIF
                     MGDTGSLFLGFMLTFYLFEFCEMNAYQSVPQSMYMAAAPAVAICVLIVPLYDTLRVVI
                     TRLKKGVSPFKADKNHIHHLLLKIGFSHRQVTFVLLAITAFFTAFALLARQWSNLVLA
                     FIAFLLATGMIFWLWRVINRKMQRK"
     gene            14209..16158
                     /locus_tag="PLZ54_04425"
     CDS             14209..16158
                     /locus_tag="PLZ54_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOJ66209.1"
                     /translation="MISVEQLCVEFGGTTLFDNITFLIHPKEKIALVGKNGAGKTTLL
                     RILAGKQQPTRGNISYQKDITIGYLPQHMIHSEGTTLWQETEKAFGNIVQLRQQLEDI
                     QQQLVERTDYESSAYHSLLDKLSQLTEYLQISGNGNYQAEMEKTLLGLGFLRSDFYRQ
                     CSEFSGGWRMRIELAKILLSHPDVLLLDEPTNHLDIESIQWLENFLFTSGSSLLLVSH
                     DKAFIDAVTTRTLEISLGKLYDYDVNYSKYIQLRKERHEQQLRAYENQQKLIQDTEDF
                     IERFRYKATKAVQVQSRIKQLEKLQRIEVDLEDNANMHLKFPPAIHSGKIPLEIEHLS
                     KAYREHLVLDDVNLMMHRGEKVAFVGKNGEGKSTLVKCIMNEIEYSGTLKLGYNVKIG
                     YFAQNQASLLDENLTVFETIDRVAVGEIRTKIRDILGAFMFGGENSEKKVKVLSGGER
                     SRLAMIKLLLEPVNFLILDEPTNHLDIPSKEVLKEAIKAFDGTAIIVSHDREFLDGLV
                     TKVYEFGNKKVKEHLGGIYDFLQAKKMESLHELERNVSSEVKNEGEAKPESENKLNYE
                     SRKELNRKIRKLEKDVEATEGLITDLDNEIQQMNTDLQIPEKASDPEFVSTLQKKRAE
                     LERKMYEWEILSEELEKIRKEWQGQ"
     gene            16226..16930
                     /locus_tag="PLZ54_04430"
     CDS             16226..16930
                     /locus_tag="PLZ54_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOJ66210.1"
                     /translation="MKTLYLIPTPLGNTSFDRIFPPVNLEIIRNLRYFIVEDIRTARR
                     FLKQVDVEIDIDKLFFYTLNEHTSPTEVDGFLQPLREGEDMGLLSEAGCPAVADPGAD
                     MVAIAQKNAFRVVPLVGPSSILLALMASGFNGQNFAFVGYLPVSPAERIKAIRKLESR
                     AYSENQTQIFIETPYRNLKLFEQILQNCRPSTLLCIAANVTLPDESIVTRSVAEWKTI
                     TPDIDKKPCIFLIYKG"
     gene            complement(17303..17485)
                     /locus_tag="PLZ54_04435"
     CDS             complement(17303..17485)
                     /locus_tag="PLZ54_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66211.1"
                     /translation="MSRYSKHSEQRFRNQFKKKFGFITYNSSLIKPYLGKRTVISFDP
                     SHITKSGKKTPYLGSF"
BASE COUNT         5311 a         3388 c         3335 g         5652 t
ORIGIN      
        1 gaatcaacgg ataaacaaca taaatctgtc ttccggcttc aatttgtttc accataaatt
       61 tataaagcgc ctgccttttg ttgtcgtaga aatgaaaagt ctgaacaggt tttctgccgg
      121 gcggcaattc atcgataacg gaaacagtaa ggtcgccgta aatggtcatg gccagggtac
      181 ggggaattgg tgtggctgtc attaccagaa tatgcggagg ttgaacattt ttactccaaa
      241 gtcttgcacg ctgagccacg ccaaaacgat gctgttcgtc aatcacaacc aaccccagat
      301 ttttgaactg aaccgtttct tctatcaagg catgtgtacc cagcagaata ttcaattcac
      361 cgctcagcag cttttcgtga atcgccgctc gttcctttgg tcgcgtcgaa cccgtgagca
      421 aagcaatttg gataccaata tttcccaaaa actgcttcaa actttcaaaa tgttgatggg
      481 caagaatttc ggtgggagcc atcatacaag cctgaaaacc attatcaacc gccatcagca
      541 tggcaaaaag tgctaccagc gttttcccgc ttcccacatc tccttgaagc aatctgttca
      601 tttgtttgcc ggaagcaaca tcggagcgaa tttctcgaat gacttttttt tgggcattgg
      661 tcaactcaaa aggcaaataa tcgcgataga aagtattgaa ataatagcca acatgtttga
      721 aaacaaaccc tttaaaccgc tgatttcgcc atgcagtttg tttcacaata tccaactgaa
      781 tgtaaaaaag ttcttcaaat ttcagtcgct gtcgtgcgcg attcaacaaa tgactgtttt
      841 ttggaaaatg tatgttgact aaggcatctt tcaggttaag caatccataa cgattcagaa
      901 tgtatgccgg caaggtttca ggaattccat tttgcaaagt cggcaacagt gaataaataa
      961 ttttctgaat ggctttggaa ttcagaaaat gagtcttcat tttttcggaa gtattgtaga
     1021 aaggctgtaa gcccatttgc tcaaccgggg gaagttgtga aagaatttcg atgtcgggat
     1081 gaactatatt gtactttccg ttgaaaagcg taggttttcc gaaaacaaca tattcgatat
     1141 tggttttata acgttctgct acaaatttca aacctttaaa ccacaccagc tcgatatatt
     1201 gccttccatc ggtaaatacc ccaatcaaac gctgactgca tccttcacca accttctcaa
     1261 acttcagaat tttccctttc agttggatga aaggcatatt ttcggaaatt tcgctgatca
     1321 tgtaaaaacg acttcggtca acgtatttat aaggataatg tctcagcaaa tcctcagccg
     1381 ttcggatgtt caattccttt ttgaaaagct cggcccgctt ggggccaacg cccggaataa
     1441 attgtatgtc ttgggtaaaa aaatccactt atgtaaaaaa tcttcaaaaa attttccaaa
     1501 tcaataactg ctgttttctc ttttcgccaa cagaaaagat gccaattcca aatcaaaagc
     1561 tgtagtcagt ttaatatttt cccgatttcc ttcggtgaga tgaacattta caccgatagc
     1621 ttcaaccaca gaagcatcgt cggtgaaaag tggagaaaac tcctgctcat aagccttttt
     1681 caaaatgtta aaatcaaaaa cctgcggcgt ttgcaccagt ttgtaggcac ttctgtccaa
     1741 cgattggctg tacttggtat gaattgtgcg aaggctatca accaaatcca tactcggtat
     1801 ggccgaacca aatttttcgg cttcgtcaaa acaacgccga atggtctcca cacttaccaa
     1861 aggccgaaca ccatcgtgca cagcaaccaa tgctgaatta ttctcttcct cagatttatc
     1921 cggataaagc gacgctaaaa aagcagcaac agcttccaac ccgtttttta cggaatgaaa
     1981 acgcgtttcg ccaccaataa ccacttggtg agaaaatttg aaagaatatt tcgaacaaag
     2041 atttttccaa aattcaattt gagttacagg caaaaccaca aatacaggta aatcgaaatc
     2101 gtaacgtgca aaagcttcaa tggtgtgcat caatatgggt tttcccgcta tttcaataaa
     2161 ctgttttgga atgtcggact gcatgcgttc accttttccg ccggcaacaa taagcacaat
     2221 ttttgtaagt tccataaatt gggaaaaata gttacgaaca aagataaaca ttaaacttca
     2281 aaaaatgatg accaaattta gttcaatctt tccattattt cataatttcg tcgaaaagct
     2341 atcggattta cccgatattt tataactttg cactccaatc atgaatgaaa tgatgaaaaa
     2401 ttttaaattt ttacttttca caatttttct cgcagtgtct ggcgggcttc aggctcagga
     2461 tgaaagcaca tctgttcgac agaatatttt tgaagttctt caagcatcgg atggagcagg
     2521 caaagttatc attcatcaag acaagcgcat agaaaatctt atcaatcaaa agcaaaccgc
     2581 caaaacgggt cgtgaagcga cgttggggaa tggttaccgc gtgcaggttt tttcgagcaa
     2641 cgaacagcgc accggaaaag ttgaagcttt caaaatagaa aaagaaatac gtgaggcttt
     2701 ccccaatacc ggcgtttatg tcaactacaa ctcgcctttc tggaaagttc gcgtaggcga
     2761 cttcagttcg gtagatgaag cgcaagcctt tcgcaacaag ctgctggaag tctttcccga
     2821 cctgcgaagt cagacttatg tggtgagaga tcagattgta aaatagcttt ttgaaaaaaa
     2881 atcagtttca tggatttcga cattaccagt tcccgtcctt ttgacgaaaa aaagacagga
     2941 gaaattgcac aacattatca ggaaattatc cgtctgctgg gcgaaaaccc cgaccgtcag
     3001 ggtttgctgc aaacgcctca gagagtggca cgcgccatgc agtttttcct acaaggttac
     3061 gaacaggacg ctgaagaaat tatacgcacg gccactttca cagaaaacta ccgtcagatg
     3121 gttattgtta aggatataga tttttattcg ctttgcgaac atcatttgct tccttttttc
     3181 ggaaaagcgc atgttgccta tatccccaac aaaaaaatta ccggtttgag caaaatagca
     3241 cgcgtagtag aaatttatgc ccgtcgattg caagtacagg aacgtatgac cacacaaata
     3301 aaacaatgta ttcaaaacac gctcgatcct atgggggtaa tggttattat cgaagcgcag
     3361 catctgtgca tgcagatgag aggaatacaa aaacaaaatt ctattacaac cacttccgat
     3421 tttacaggcg tttttcagga agcgcgcaca cgtgaggaat ttatgaattt gattaaccga
     3481 tagcccgaaa tcataaaaca tcaactttcc cctacacgca gcaacataca attaaaagct
     3541 atatttaaaa gtctttttgt aagtttttta aaaacaagac aaaagtttaa acaatctttt
     3601 taaatgttcc tttttcccaa agacaaatca aattagagtc cgctccgaaa agtcaaaaaa
     3661 gctaaattca gcaacttttt gcccttaatt taaatgtagg ttgctgaaaa atgagttttc
     3721 tttatcgttt gggcgaagcg aattttcaaa aataaaactt ttcggagcgg actcaaatta
     3781 tatttatccg caatgcgaat atccacagtt tttgcaggtt aaacaacctt cttgatagat
     3841 cagtgttttt tgtccacagg aaggacaatt tttaccatta gcttctgttc catccggaat
     3901 atattttttc agggcacgtt ctactccaac tttccaagtg ttgattgatt cgctgtccaa
     3961 ctgcagtccc gaaaccaatt ttattacctg gtcaatcggc attccgtaac gcaacacgcc
     4021 tgaaatcagt ttggcataat tccaaaattc tttgtcaaac ttatgggaca agccctcaat
     4081 ggttgtttta taccctcttt tgttggtaaa ctgaaaatcg tatcttttgt tgccgttttc
     4141 gtccaccgtt ttaattattt ttccctgagt aacggtttta ggcaatgcaa ttccttcttc
     4201 gtcatcagcc aatccggtaa aaatttcata aggacgccca tctttcagcc ctacaaaagc
     4261 aatccattta tctttggcat tctgaaatct gaccacatcg caatccaatt ctttcggacg
     4321 aattaaatgc tcttccgact gaggaaagca aatttttctc tcgcctgatt tctcggattt
     4381 tttttcttct accgacagca aaacgccggc tcgtgaacct tcacgataaa cggttactcc
     4441 cttacatccg caacgccatg cttcttcata aagttgacca accagctctt cggttgtatc
     4501 ggcaggtaaa ttgatggtta cgctaatgga atggtccacc catttttgaa ttcgtccctg
     4561 catctgaact tttttcaacc agtcaatatc gttggctgtt gctttgtaat agggagattt
     4621 ttccaccaat tcgtcaagtt ctgctttggt atacttctgg gtagtggaat aatgattggc
     4681 ttccatccat gtaacaaact tatgatgaaa aaccacatat tcttcccatg aatcgccatt
     4741 ttcgtccaca aaatctacgc gaacattttt atcattggga ttcacttttc gacgacgggt
     4801 ataaaccggc ataaaaaccg gttcgatacc tgaagttgtc tgtgtcatga tgctggttgt
     4861 tccggttgga gcaatggtaa ggcaggcaat gtttcttctt ccatgtttta ccatttcatc
     4921 gtacagttca ggatcggctt cacgtaaacg gagaatatag ggattttttt cttcgcgttt
     4981 tgcatcataa atgctgaaag caccgcgttc cttggcaaga gtcacagaag aacgataagc
     5041 cgccaaagca agaattttgt gtacctgttc agaaaaatct atggcagaat tggttccata
     5101 acgtaaaccc aacgcagcca acatgtctcc ttcagctgtg gtacccacac cggtacgccg
     5161 acccatagaa gttttatttt taattttttc ccaaagtttg agctccgtgc gtttgatttc
     5221 ttcatcttcg ggatcggcat ttattttggc cataatttta tcgattttct ccatttccag
     5281 atcaataata tcgtccataa ttcgctgagc tactgcgaca tgcttcttaa acaaatcgaa
     5341 atcgaatgaa gcttccggcg taaatggatg attgacatac gaatacagat taatagccag
     5401 cagtcggcag ctatcgtatc cgcacagtgg aatttcacca caaggattgg tagaaatagt
     5461 tttgaaaccc aaatcggcat aacagtcggg aacagattca cgggtaatgg tatcccagaa
     5521 aagtacgccc ggttcagccg attgccaagc attgtggata atttttttcc acagggaatg
     5581 ggcatctatt tcttttttga accacggatg gtcatcctct ataggaaatt gctgagtata
     5641 agtttcctgc gccaaagcag cctgcataaa atcatcgtga attttcaccg aaatattggc
     5701 acccgtaatt ttccctgctt ccaattttgc atcgataaac gattccgaat cgggatgttt
     5761 aatggaaacc gtcaacatca aagcaccacg acgaccatcc tgtgcaactt cacgagtaga
     5821 attggaataa cgctccataa aaggtacaat tcccgtagaa gtaagcgcgg aatttttcac
     5881 cggcgaacct ttggggcgaa tgtgagaaag atcatgcccc actccacccc gacgcttcat
     5941 caattgaacc tgttcttcat caattttaat gataccgcca taagaatcgg catcatggtc
     6001 aaaaccaatg acaaaacaat tggaaagtga agctatttga aaatcgtttc caattccggt
     6061 catggggctt ccttgtggaa cgatatactt gaaatgccgg aaaagttcaa aaagttcatc
     6121 ttctgtcagc ggattgggat aatgtttctc gattctggca atttcacgag ccaatctttt
     6181 gtgcatgtca tccggcgtca actcgtaaaa attcccgtaa gaatctttta acgcatattt
     6241 tgttgaccaa acagtggcag ccaattcatc tccctgaaaa tattcaaaac tggctttttt
     6301 tacttcttcg gcagtataag tttttggttt cacaacaaaa atttttaatt gggttaataa
     6361 ataaaggttt aagaaaaatt agcgacgcat tttttacaac tgcaagttta taaataaaaa
     6421 aatgattatc caataatttc aataattatt tttccttttc ggtaaaaatt ttctatttca
     6481 aaaattatat tattgattat taataattta actttttata ttaaataaaa aatttcctta
     6541 tttgaaaact tttgcatata attaattttc aataaaggaa atctataacc taataatata
     6601 aatagaaaaa agatagatga gattaataaa acttacaaaa ttattccatt ctctacctta
     6661 aaaattttgg cttcctgatc gagttggtaa agaatttgat ccaaatgttc gcgattggtg
     6721 tcggtaataa aaatctgacc gaaagtttcc tgtgaaacaa gttcaacaat ttttctaaca
     6781 cgatcggcat cgagtttgtc aaaaatatca tccaataaaa gtatcggtgg ttgatgatgg
     6841 gtttgtttaa gaaatttgaa ttgagccaat ttcaacgaaa ccaaaaaagt tttattttgt
     6901 ccttgtgaac ccacacgctt aatcggataa ccattcaaca acatttccaa ctcatcctta
     6961 tgaatgcctc gtgtggaaaa accaagcatt ttatcccgtt cacgggttga cttcatttgt
     7021 ttttttatat cgttttgctg aaaatgcgac aaataagtga tgtcaatctc ctcattttca
     7081 tgggaagaaa tatagcgata gtactgttga aacacaggaa tcaactgatc gataaaacgg
     7141 gttcgttgtt caaaaatgta agaaccatag tcggccattt gttcttcgag aatttcaaac
     7201 aaagattcgt cgacgggcgt ttcgaccttc aacaaggcat ttcgctgctt cagcgcattg
     7261 ttgtaaagca ataaattatg cagatatttt ttgtcgtatt gcgaaatcac gccatcaaca
     7321 aactttcttc tctcgtcgct gccttcggaa ataaggaccg aatcttcggg cgaaatcaat
     7381 accagcggta acaatccaat gtggtcggat agacgttcgt agtccttttt gttgcgttta
     7441 aattgctttt tctgattgcg tttcattcca caataaatct cctctgttgc ttcgcccata
     7501 cgatattttg cctgcaatac ggcaaaatcg gcctcatgaa aaatattttg tgaatcaata
     7561 aagttggaat gacttttaca aaaagacaaa aaatatacgg catccagcaa attggttttc
     7621 cccatcccgt tgttgccaat aaagcaattg attttcgggg aaaagctcaa ttcagcctga
     7681 cggatgtttt tataattgac aatgttaatc gattcgatgt acatgaatgc taaatcaaaa
     7741 ctttgtgttc acaaatttac gggataattt ttgaatttca cacggtaaaa aacaaaaata
     7801 catgaataac gctgacgtcg gcttctttag catgaaattt cgaaattcag gggattaaac
     7861 caggattaag ctttgacaat ttaaaaaagg aaagtcaagc tcagaagagc tgtcccatat
     7921 tcatcaaaac catgctatcc ctttttggta aaaagcaatt ttgcatcaat gtttattatg
     7981 aatcatttca aaacgcatcc ggaggccaaa agtatttatc gcataaaaaa cttttgattc
     8041 gcaaacaggt aattggttag accaaaccga acgaaataga aaggatacca tcagagaata
     8101 aggaggctgc agaaattatt ggtttgtgta taatttaaat ctaaaaattc tgcaagcctg
     8161 tttggaaatg gaaattatga atgatgagcg gcaaaacgat tgaaaataat taaaaatgct
     8221 cgaccatttt tcctatgatc gagcaattgt ttagaaagaa aaaattatta aatacgttcg
     8281 agattttcgg ttgtaatttt ttcttctaca atggcatcaa gaacagcgat agcagtcata
     8341 ttgacaatat cgcggacgga gctttccaca tccaaaatat gtacgggctt attcagtccc
     8401 atttgaattg gcccaacagc ttccgagatt cccatttcga gcaatagctt gtaagcagaa
     8461 tttgcagaac ttaaattggg gaaaatcaat gtattgacat ctttccctgc taatctggta
     8521 aaaggatact tgcggtcgcg caattctttg ttcagtgcaa aattcacttg catttcaccg
     8581 tcaactatca tttcaggtac ttcgcggtgc aatgtttcaa ctacttcgtg aacgctggct
     8641 ggacttccct gtttgtcgga accaaaattg gagtaggaaa gcatggccat caccggttcg
     8701 tgtgcaaaga attttaccgt acggtaagtt aatttggcaa tatcgattaa agtttcggta
     8761 gaaggatgac ggttaaacaa tgtgtcggcc atgaaaaaag ttcctttttt tgtattcata
     8821 atgtgcatgg ctgcaatatg tttggaccca ggcctgatgc cgataacgtc ctttgctgcc
     8881 tgaatggcat cggcatattt tgtataaact ccagtgataa gggcatccgc ttctccggtt
     8941 tcaaccatca tcatgccgaa ataattgcgt tcatacattt tttcataagc ttcatcgtaa
     9001 gtcattcctt cgcgtcccag tttttttgca agtttttcgg cataggcaat acgtcttgct
     9061 tcttctctgt cgtggcggag attaattatc tcgattcctg acaagtcgat gttgtattcg
     9121 cgggctatgc tgttgatttt ttcttcatta cccaacagaa cagggaaaca aacaccttcg
     9181 ttgtaagcca tttcggcagc tttaagcata ttcagatggt ttgcttcgga aaatacgacc
     9241 cgttttggat tttgacgtgc tgtttcgtaa aagcttcgca gcattttatc gtcataaccc
     9301 atcgtttcac ggagttttgc attatagccg gcccagtcgg taatgtgttt gcgtgcaacc
     9361 cccgattcca tcgctgctcg agctacagcc ggagccacaa tggtaagtaa gcggggatcg
     9421 agaggtttgg gaataatata atcgcgtcca aaacttaatc ttttcaagtt gtaggcagca
     9481 ttcaccacgt caggaactgg tttttttgcc aattcagcaa gtgcataaac ggcagccaat
     9541 ttcatctcct cattgatgca ggttgccctc acatcaagtg ccccacggaa aatataagga
     9601 aatccgagta cgttgtttat ttgattggga tgatcggaac gaccggttgc aaaaataata
     9661 tcttcgcgtg ccgagatagc atcttcataa gatatttcag gatttggatt ggctaaggca
     9721 aaaacaatgg gattttcatt cattgatctt accatttcaa tagacagaac gccggcaacc
     9781 gataaaccta aaaatacatc tgctcccttt acagcttctt ccagtgtatt aatttctctg
     9841 tcggtagcaa aggctttttt cgtttcattc aggtcggtgc gttgtttgtt aattactcct
     9901 tttgagtcga gcatgacaat attttcaggt ttagcgccca atctgatata taattttgtg
     9961 caggaaacag ctgcggctcc ggctccatta acgacgattt taacgtcttc cattcttttg
    10021 ccggcaattt caagtgcatt aatcagtccg gcgcccgaaa taatggctgt tccgtgctga
    10081 tcgtcgtgca taatgggaat atccagttct gcttttaatc gttcttctat catgaaacat
    10141 tccggagctt taatatcttc caggttgata ccgccaaaag taggtgagat agcttttact
    10201 gcctgaataa atttttcggg atctttttcg tttacttcaa tgtcgaaaac atcaataccg
    10261 gcgaaaattt taaacagcaa agcttttcct tccataaccg gtttccctgc catcgcgcca
    10321 atatcgccca gccccaatac ggctgtaccg ttggaaatta ctgctaccaa attacctttg
    10381 tttgtgtaat cgtaaacagt agcgggatct ttttctattt ccaaacaagg ctccgctacg
    10441 cccggcgaat aggcaagcga taaatctctt tgagaactgt gaggcttggt aggaacaact
    10501 tcgatttttc ccggttttcc ttgcgaatga taaaggagcg catcttcttt ggttaatttg
    10561 gtcatatagt tttttgaatc aattagaaat taaaaaatat taatgggttt gttttaaaaa
    10621 ttgtcgtaaa attacatctt tttatgacga tgtgcaatta taaacttaac tctttctata
    10681 ttttccgaaa agaactgctt ccttttaagg aaaaataaat tttcttaacc cactttctgt
    10741 attttgtctg aacttatcgt aaaaaaattt tatatcaagc ctttttgcca atctatggaa
    10801 ttagtttttt aaaattgaaa caattttaac tgtatactgt ttataattca gctttaattc
    10861 actcccttcg attttttttt cgaaatttat tgacaagaac tttatatttt taaattttta
    10921 aacttatctt tgcattgctt ttgactgtgc acaggaaata accttacaaa aatttatcct
    10981 tttggttttt tatgaaagag atattcaaat ttttaaacaa tacggatacg ttctctcacc
    11041 tggcttcttt aaaatattta cctcgctggg tcgttttatt tatagatatt acaatatctg
    11101 ccatatcttt tttagcagct tatctcgttg cttcatcttt cataaaacac ttgcctgacc
    11161 aacttctttt acctcttttt ccacgtttga ttttattgct ttttacacaa agtgtttttt
    11221 tctggctttt tcatacttat tccggtatcc tcagatattc tacaatgaca gatgctgcca
    11281 aacttttttt ggcaatattt ggtaattgta ttttcctctc agcgttgaac tttgtttgtt
    11341 ctctttattt tcctgcacca cttttttatt atgcatccat tctgatttat ggtgttatta
    11401 gttttattct gttattcggt ctcaggctca ccgtaagaaa tattttcaat tattttgttg
    11461 aaacctcggc tcagatcatt ccggtgatga tttatggcat cgatgaggcg ggtctgggta
    11521 tagccagaat gattctttcc agtgtgaatt ctaaaaaaca atataaattg gttggtttca
    11581 ttgatggcaa tgaaaatatt gtcaacaaaa tggtaatggg attgagagtg tataaacatg
    11641 atgatcatac tattaaaact aaagttgcca ataaagtaaa taccgttatt gtttctccca
    11701 taaaaatgaa ggaaattaat ccggatgtgg atttggatat atttattcag catcatattt
    11761 cattgctaac atctcagccg atggatattt gggaaggaga aatgccttcc gtgagacaaa
    11821 tcaaaccgtt gaaaatagaa gatttattgg agagagaacc gattcagatt tcttttgaca
    11881 atattgctgc cgaattgaaa tcgaaaactg tgatggttac cggagcagca ggttccattg
    11941 gcagtgaaat tgtccgtcag ttgtgtaatt ttactccaaa gttaattgtg ttggtagata
    12001 atggagaaac ggccattcat aacctgaaaa tcgaattgca agccttttct acatctcaac
    12061 attttaattt tgtattgggt gatgttcgga ataaagagcg gatggagtat attatggatt
    12121 tatatcgtcc cgattttgta tttcatgctg cagcctacaa acatgttcca atgatggaag
    12181 attttccctg tgaaagtgtt caggtgaatg tgctgggcac caaaattatg gccgatttgg
    12241 cattgaagta tagagtttca cggtttgtca tggtttctac cgataaagca gtgaatccga
    12301 ccaatgtgat gggagcttcc aaacgtatcg ccgaaattta cgtgcaatca ttggccagaa
    12361 aaatgcagaa aaccggaact gaaaccacaa aatttattac tacgcgtttt ggaaacgtat
    12421 tgggatcgaa tgggtcagtg gttccgtttt ttcaaaaaca aatcgaaaaa ggaggacccg
    12481 ttacagtaac acatcctgat attattcgtt atttcatgac tgttcccgaa gcaagcagct
    12541 tggtgttgga agcgggtgta atgggcgaag gcggagaaat ttatgtattt gatatgggaa
    12601 atccggtaaa tatcagtcat ctggcaaaga aaatgattca aatggccgga ttaattcccg
    12661 gtgtggatat tcagatagct tacaccggcc tgcgtccggg tgaaaaatta tacgaagaac
    12721 ttttaagtca taaagaaaaa gtgagatcta cccaacatcc aaaaattatg gttgcttcgg
    12781 ttgcagaata cgattttgat aaaatctccg ttgatataga taacttgatt aaatacagct
    12841 atctctgcaa ggattttctg gttgtatcca tgatgaagca gattgtcccg gagttcaaaa
    12901 gtaataattc tcaatacgaa ttattggata tcagtaatta agacacaagt ttaagcagat
    12961 cttttggttg taagcgtttg aaaataatta atttcttttt tcattatttt cagacgattt
    13021 tttattgatt tttttttttc gcagaaacag agaaataaat atacttaaca acacaaaatg
    13081 aattatttat ataattttat tcaacaaaat ccttatttgc tgattctttt ttcttttctt
    13141 ttgggaaaag ctttcatgcc ttccgttgtt agaattgcca agaaacataa ttttgtggta
    13201 aaacccaaca aacgaacaac tcataaaggt ttgataccct atacgggcgg ttttgatatt
    13261 tttatttctt ttatctttac aattttaatt ttttgtttcc ctttgatgga gaaattacaa
    13321 tatatcccca ttggtatttt cattattttg atgaccggtt ttattgatga cttgctgaat
    13381 attaaaccca catccaaatt attgggagaa tttgttgcag gagtttttct tattgtgttt
    13441 tccgacatca gaataactaa tttttttggt gttttcggta ttcatgaact tcctttgtcg
    13501 attagttata ttttttcttt tttggttttt ttactgatta tcaattccat taatttaatt
    13561 gatggggtag atggtttggc ttccggactc ggaatttttt acaatctttt tttcggcatt
    13621 tattttcatc tgaccggcaa tttgtcactg gccatgattg cttatacgct tacgggttcg
    13681 ttggccgtat tttttattta caacgtattc agcaagaaaa acaaaatttt tatgggagat
    13741 accggttccc tgtttttggg atttatgctg actttctatc tctttgaatt ctgtgaaatg
    13801 aatgcttatc agagtgttcc tcaaagtatg tatatggctg ctgctccggc tgtagctatt
    13861 tgtgtgctga ttgttccgtt atatgacact ttgcgggttg ttattactcg tttaaagaaa
    13921 ggggtttctc catttaaggc cgataaaaat catatacatc atttattgct gaaaattgga
    13981 ttttctcacc gacaggttac attcgtttta ttagctatta ccgccttttt tacggctttc
    14041 gcactgttag ctcgtcaatg gtcaaacttg gtgttggcgt ttattgcctt tttgttggct
    14101 acgggtatga ttttttggtt atggcgagtt ataaacagaa aaatgcaaag aaaataactg
    14161 cttgcaagtt tattcttaca ttttctattt tttacttttt gaatatttat gatttcagtt
    14221 gagcaacttt gtgtcgaatt tggcggaacc acactttttg ataacattac ttttctgatt
    14281 catcccaaag aaaaaattgc gttggtaggg aaaaatggtg ctggtaaaac tactttgttg
    14341 cgtatattgg caggcaaaca gcaacccaca cgcggaaaca tatcttatca aaaagacatt
    14401 accattggct atctacctca acacatgatt catagtgagg gaacaaccct ctggcaggag
    14461 acggaaaagg cttttgggaa cattgtgcaa cttcgacagc aattggaaga cattcagcag
    14521 caattggttg aacgtacaga ttatgaaagt tcagcatatc attccttgtt ggataaactc
    14581 tctcagctga ccgaatatct tcaaatttcc ggaaatggca attatcaggc cgaaatggaa
    14641 aaaactttgt tgggacttgg ttttctgcgt tccgattttt accgacagtg ttcagaattc
    14701 agtggaggtt ggcgcatgag aattgaactt gccaaaattt tgctttccca tcccgatgtg
    14761 ttattactcg atgaacctac caatcatttg gatattgaat ccattcaatg gctcgaaaat
    14821 tttttgttca cttcgggcag ctctctttta cttgtttctc acgataaagc ttttatagat
    14881 gcggttacta cacgtacttt ggaaatttcg ttgggaaaac tgtatgatta cgatgtgaat
    14941 tattcaaaat atattcagtt gcgcaaagag cgacacgaac aacaattaag agcttacgaa
    15001 aaccaacaga aattaattca ggatacggaa gattttattg aacgtttccg atacaaagct
    15061 accaaagctg ttcaggtaca atcgcgtatc aagcaactcg aaaaattgca gcgtatcgaa
    15121 gtggatttgg aggacaatgc caatatgcac ttgaaatttc cgccggcaat acattccgga
    15181 aaaattcctc tcgaaataga acacctttcc aaagcttaca gggaacattt ggtgctggac
    15241 gatgtaaatt tgatgatgca tcgaggagag aaagtagcat ttgtcggaaa aaacggagaa
    15301 gggaaaagta cattggtaaa gtgtatcatg aatgagatag aatattcggg aactttaaaa
    15361 ttgggataca atgtaaaaat cggctatttt gctcaaaatc aggcttcttt gctggacgaa
    15421 aatttgacgg tttttgaaac catcgaccgc gtagccgttg gagaaattcg tacaaaaatc
    15481 cgggatattt tgggcgcttt tatgtttggt ggcgaaaatt ccgaaaaaaa agtcaaggta
    15541 ctttccggtg gtgaacgcag cagattagcc atgataaagc ttttactcga acctgtgaat
    15601 tttttgattt tggacgagcc aacgaatcat ctggatattc catcgaaaga agttcttaaa
    15661 gaagccataa aggcatttga cggtacggca attattgttt cgcacgaccg tgaatttctg
    15721 gatggtttgg taacaaaagt gtatgagttc ggcaataaaa aagtgaagga acatttgggt
    15781 ggtatttacg atttccttca ggcaaaaaaa atggaaagtt tacatgaact tgaaagaaat
    15841 gtatcttctg aagtgaaaaa tgaaggtgaa gcaaaaccgg aaagtgaaaa caaattgaat
    15901 tatgaaagtc gcaaagagct gaatcgaaaa attagaaaat tggaaaaaga tgtggaagca
    15961 accgaaggcc tgataacaga tttggacaat gaaattcaac aaatgaacac ggatttgcaa
    16021 attcccgaaa aggcttctga tcccgaattt gtttcaactc ttcagaaaaa gcgtgccgag
    16081 ttggaaagga aaatgtatga gtgggaaatt ttgagtgaag agttggaaaa aattagaaaa
    16141 gaatggcaag gacaataaat ctcttttcgt tccattgtcg gtaattcgtt tttaaatttc
    16201 aatcaatgta ttaaagccga tttttatgaa aacgttgtat ttaattccca ctccacttgg
    16261 caatacttct ttcgatagaa tttttcctcc tgtcaatctg gaaattataa gaaatttacg
    16321 ttattttatt gttgaggata ttcgtactgc tcgtaggttt ttaaaacagg tcgacgtcga
    16381 aattgatatt gataaattat ttttttatac gctgaatgaa catacttctc ccactgaagt
    16441 tgacggattt ttacagcctt tacgggaagg cgaagacatg ggacttctct ccgaagccgg
    16501 atgccctgct gtagccgatc ccggcgccga tatggtagct atcgcccaga aaaatgcttt
    16561 tcgcgttgtg cctttagttg ggccttcttc aattttgctg gcgctaatgg cttccggttt
    16621 caacgggcag aattttgctt ttgtcggata tttgcccgta tcgcctgcag aaagaataaa
    16681 agcgattcga aaacttgaat caagagccta ttccgaaaat caaacgcaga tttttattga
    16741 gacaccctat cgtaatttaa aactttttga acaaattttg caaaattgcc gtccttccac
    16801 tttgctttgt attgcagcca atgttacttt gccggatgaa tccatcgtta ctcgaagtgt
    16861 tgcagagtgg aaaacaatca ctccggatat tgataagaaa ccatgtattt tcctgattta
    16921 taaaggttaa aagcacttca atctaatttt ttttggttga taagaaattt tcctcctcac
    16981 tttgacaatg cgtcacccta aaaatttttc aaaatttcat atcacatagg ggtgagattg
    17041 atttgtgttt tttagttatc aaaattgctt tggtcattgt ttcactactc ttagatagat
    17101 taattttcag agtagtaatt gttttggcta tgttgaaaac tttatccaca ctcaaatcgg
    17161 acaatgtcag ggaaactgtc tttagcgttt ctaccggtgg tgtttgtgct gcttccaaat
    17221 ggaagctctg atttgagtca atatccagca gagaaaattc agctatttca agaccttttg
    17281 tgatacattg ctcggaatct gatcagaacg aaccaagata gggtgtcttt tttcctgatt
    17341 tagtaatatg actcgggtca aaagaaatga ctgtcctttt acccaaataa ggttttatca
    17401 gagaactgtt gtaggtgata aaaccaaatt ttttcttgaa ctgattccga aaacgttgct
    17461 cgctgtgctt actgtaacga ctcatttgct taaaatttat tcgacccgga atgatgaggt
    17521 ataacataaa aatttccaat aaaaagtttt gacgccattt gttgattgat aaagaatgaa
    17581 agccgctgct gactgctgaa ataaaagtgt taatttcctc gaaaatcata tcttatatac
    17641 attacattaa attattgcac aaataaatta ttatctttgt ctcata
//
LOCUS       DAOSCH010000038        17647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2222_length_17647_cov_6.358572, whole genome shotgun sequence.
ACCESSION   DAOSCH010000038 DAOSCH010000000
VERSION     DAOSCH010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17647
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2222_length_17647_cov_6.358572"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /gene="rplB"
                     /locus_tag="PLZ54_04440"
     CDS             complement(<1..623)
                     /gene="rplB"
                     /locus_tag="PLZ54_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOJ66212.1"
                     /translation="MAVRKLLPTTPGQRNKIISKFDTITKREPEKSLVYGKRKTGGRN
                     NNGKMTMRYIGGGHKKKFRVIDFTRKKDGIPATVKAIEYDPNRSARIALLYYADGEKS
                     YILAPNGLQVGQKIISGSNVAPEVGNALPLENIPLGTIVHNIELRPGQGAAIARSAGT
                     FAQLVSREDKYVIIKLPSGEVRKILGACKATVGSVGNSDHALEKSGKA"
     gene            complement(633..923)
                     /gene="rplW"
                     /locus_tag="PLZ54_04445"
     CDS             complement(633..923)
                     /gene="rplW"
                     /locus_tag="PLZ54_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOJ66213.1"
                     /translation="MGVILKPLVTEKLTKLGDKFNRYGFKVQKDANKLQIKQAVETMY
                     NVTVVDVNTMIVPSKKRSRYTKSGVISGKRPAYKKAIVTVKKGEEIDFFSNI"
     gene            complement(938..1570)
                     /gene="rplD"
                     /locus_tag="PLZ54_04450"
     CDS             complement(938..1570)
                     /gene="rplD"
                     /locus_tag="PLZ54_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOJ66214.1"
                     /translation="MELSVLNIKGEDTGRKVSLNDAIFGIEPNDHAIYLDVKQFLANR
                     RQGTHKAKARSEISGSTRKLGRQKGGGGARPGDIKSPLRVGGGRVFGPVPRDYSFKLN
                     KKVKQLARKSALSYKAKNGAITVVENFEFEAPKTKKMLELAKNLQVADKKPLIVLSSA
                     NKNVYLSARNLPSINVITTSELNTYNVLNAKTLVLTEGSVTILEQIFKVE"
     gene            complement(1573..2190)
                     /gene="rplC"
                     /locus_tag="PLZ54_04455"
     CDS             complement(1573..2190)
                     /gene="rplC"
                     /locus_tag="PLZ54_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOJ66215.1"
                     /translation="MPGLIGKKIGMTSVFSADGKNVPCTVIEAGPCVVTQIKSIETDG
                     YEAVQVGFEEKKPKHTNKPEAGHFKVAGVAPQRHLVEFKGFEGDFKLGDVITVEIFDG
                     STFVDVVGTSKGKGYQGVVKRHKFSGVGETTHGQHNRQRAPGSLGASSFPSRVFKGMR
                     MGGRMGGDKVTVQNLQVLKIIPEHNLLLVKGSVPGAKGSIVIINK"
     gene            complement(2310..2615)
                     /gene="rpsJ"
                     /locus_tag="PLZ54_04460"
     CDS             complement(2310..2615)
                     /gene="rpsJ"
                     /locus_tag="PLZ54_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOJ66216.1"
                     /translation="MSQKIRIKLKSYDYNLVDKSAEKIVKTVKATGAVVSGPIPLPTH
                     KRIFTVNRSTFVNKKSREQFELSSYKRLIDIYSSTSKTVDALMKLELPSGVEVEIKV"
     gene            complement(2685..4808)
                     /gene="fusA"
                     /locus_tag="PLZ54_04465"
     CDS             complement(2685..4808)
                     /gene="fusA"
                     /locus_tag="PLZ54_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOJ66217.1"
                     /translation="MAKVDLSYTRNIGIMAHIDAGKTTTSERILFYTGLTHKIGEVHD
                     GAATMDWMEQEQERGITITSAATTTYWNYNQHQYKINLIDTPGHVDFTVEVERSLRIL
                     DGAVATFCAVGGVEPQSETVWRQADKYNVPRLCFVNKMDRSGADFFEVVRQIREVLGA
                     KPCPIQIPIGAEEKFKGVVDLIKMKAIFWHDETMGAEYSEEEIPADLVNEAREWREKM
                     LEIVAEYDDAVMEKFFDDPSKITEDEIKAAIRKGTLAKEINPVICGSAFKNKGIQTML
                     DAVCAYLPSPLDNPEIVGKDPRYEDKTVVRHPDPNEPLCALAFKIATDPYVGRLCYFR
                     VYSGKLESGSYVYNPRTDKKERISRIFQMHSNKQNPVDMISAGDIGAGVGFKDIKTGD
                     TLCDENHPIVLESMDFPEPVIGISIEPKTQKDLDKLGVGLSKLAEEDPTFTVHTDEQS
                     GQTIISGMGELHLEIIIHRLRREFKVECNQGRPQVAYKEAITQPVEIREVYKKQTGGR
                     GKFADIIVRVEPADNDFEGSLQFIDMVKGGNIPKEYIPSIQKGFQSAMKNGVLAGFPV
                     DRLKVTVLDGSFHPVDSDQLSFEICAQIAFKNACTKAKPVLLEPIMKIEVVTPEESMG
                     DVIADLNKRRGQVEGMESSRTGARIIKAKVPLAEMFGYVTALRTITSGRATSSMEFSH
                     YAEVSNSLAREVVAEAKGKVELLHY"
     gene            complement(4828..5304)
                     /gene="rpsG"
                     /locus_tag="PLZ54_04470"
     CDS             complement(4828..5304)
                     /gene="rpsG"
                     /locus_tag="PLZ54_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018362998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOJ66218.1"
                     /translation="MRKSKPKRRVVLPDPIFNDPMVTKFVNHLMFDGKKSIAFDIFYS
                     ALENVKNKLPNEEKSSLEIWKKALENVTPLVEVRSRRVGGATFQVPTEIRPERKESIS
                     MKNLILYARKRGGHSMSDKLAAEIVDAFNSQGGAYKRKEDIHRMAEANRAFAHFKF"
     gene            complement(5496..5906)
                     /gene="rpsL"
                     /locus_tag="PLZ54_04475"
     CDS             complement(5496..5906)
                     /gene="rpsL"
                     /locus_tag="PLZ54_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOJ66219.1"
                     /translation="MPTIQQLVRKGRTELVDKSKSPALDSCPQRRGVCIRVYTTTPKK
                     PNSALRKVARVRLTNQKEVNAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DTAGVNGRLQRRSKYGAKRPKKGQPAAASTGKKK"
     gene            complement(6210..8003)
                     /gene="argS"
                     /locus_tag="PLZ54_04480"
     CDS             complement(6210..8003)
                     /gene="argS"
                     /locus_tag="PLZ54_04480"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOJ66220.1"
                     /translation="MNLEHTIQQLVEQALQQLYGLEIGQNPLQVQKTRKEFEGDMTVV
                     LFPFIKLLRKSPEQIGKEIGDNLLKSSSVIEDYNVVKGYLNLKISVSYWLERLNEIFL
                     QKDFWKVKLKENADLMMIEYSSPNTNKPLHLGHVRNNLLGWSIAQIQEANGWKVVKTN
                     LVNDRGIHICKSMLAWKLFGNGETPQSSGLKGDHLVGKYYVLFDQAYKQQVRELVEEG
                     WSEEEAKKSAPLMQEAQKMLLAWENNDPEVRKLWETMNGWVYEGFNETYRKLGIDFDK
                     IYYESETYLVGKKIVEEGLGKGIFYRRPDGSVWADLRDDGLDEKLLLRADGTSVYMTQ
                     DIGTAKVRYDDFPIKKMVYVVGNEQNYHFQVLSILLDKLGFEWGKSLVHFSYGMVELP
                     NGKMKSREGTVVDADDLIDEMLRTARETSEELGKLDGCSPEEVENIVRMVALGALKYF
                     ILKVDPRKNMTFNPKESIDFNGNTGPFIQYTHARIRSVLRKAQEQSISFGKLNNLNLS
                     ISQKERYLVQLLTEFPLMVKEAGDEFNPALIANFLYELVKEYNQFYHDYSILKEENTD
                     LRNFRLVLSSAVADVIKDGMALLGIEVPERM"
     gene            complement(8264..8663)
                     /gene="ssrA"
                     /locus_tag="PLZ54_04485"
     tmRNA           complement(8264..8663)
                     /gene="ssrA"
                     /locus_tag="PLZ54_04485"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8680..10200)
                     /gene="rny"
                     /locus_tag="PLZ54_04490"
     CDS             complement(8680..10200)
                     /gene="rny"
                     /locus_tag="PLZ54_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOJ66221.1"
                     /translation="MNLIIAIIISFGVGVGGSFIYFKRNHKKALKELEAEAELIKKNN
                     LIEVKEKFIALKAEHEQQVQQRNAKIQAAETRIQQREMQLNQKQGELQRKMNEVDVMK
                     ENLESQFLTVENKKKELEHLIRQAQEQLETVSGLSSEQAKERLIESLKEEAKTEAMAY
                     VNDIMDEAKMTANKEAKRIVIQSIQRLATETAIENSVTVFHIESDEIKGRIIGREGRN
                     IRALEAATGVEIIVDDTPEAIVLSAFDPVRREIARLSLHQLVTDGRIHPARIEEVVNK
                     VRKQLEEEIIEVGKRTTIDLGIHGLHPELIRLVGKMKYRSSYGQNLLQHSRETANLCA
                     TMAAELGLNVKKAKRAGLLHDIGKVPDDEPELPHAILGMKLAEKYKEKPDICNAIGAH
                     HDEIEMESLIAPIVQVCDAISGARPGARREIVEAYIKRLKDLEDLALSYPGVLKTYAI
                     QAGRELRVIVGADKIDDKEAELLSFDIAKRIQDEMTYPGQVKITVIRETRAVSYAK"
     gene            complement(10356..10652)
                     /locus_tag="PLZ54_04495"
     CDS             complement(10356..10652)
                     /locus_tag="PLZ54_04495"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOJ66222.1"
                     /translation="MNENDTFLINVQIGGYRLPLRIERKEEELYRLAEKVLGKYLEVY
                     QKKYSHRSAEEVFIFVAYELAVMLVRKMMQQDTAPLAEKVSSLNQELKKLLAEK"
     gene            complement(10660..10956)
                     /locus_tag="PLZ54_04500"
     CDS             complement(10660..10956)
                     /locus_tag="PLZ54_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66223.1"
                     /translation="MTHSYDDLVATFESNLRKLMEDFKSLQEENSLLRAQLSNKEQDI
                     ELLKVKFSELQKNYDHLRIAKTLSLSDEDRIKSKQQINKLVREIDKCLALLLND"
     gene            11150..12943
                     /gene="ispG"
                     /locus_tag="PLZ54_04505"
     CDS             11150..12943
                     /gene="ispG"
                     /locus_tag="PLZ54_04505"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOJ66224.1"
                     /translation="MWKIDPFNYQRRPSTEVRIGQLLMGGTQPIRIQSMTNTDTNNIE
                     ASLEQCIRLIRAGSELVRFTTQGNREAESLKKIHDLLRDKGYQTPLSADVHFNANVAD
                     VVAQYVEKVRINPGNYLGSNRGNITSDYSDEEYKAEYERIRKRFKQLLNICNKHQTAL
                     RIGVNHGSLSERIMNRYGDTAEGMVESCMEFLRICREENFHNLVISMKASNTLTMIHS
                     VRMLVERMEAENFHFPLHLGVTEAGNEEEGRIKSAVGIGTLLSEGIGDTIRVSLSEDP
                     EAEIPVARKLISYIDAKKNHEPIIAEKNFQHSPFNYHRRTTHAVENIGGNNVPVVIAH
                     TKENFEITPDYYFAENQIINIHNEVFPIFDIDEKEKIIKHSSRIKFLKMSFPELTKEN
                     VHFLKNTADTVVILESHHKNAPAEQKAFFHSLMNAGCTVPVIVTQSYKENIEEDLEIK
                     AGVDFGMLFVEGFGDGIFFKNEGNIPSEKLNSIAFGILQATRARISKTEFISCPGCGR
                     TMFDLQTVVSRIKSKTSHLKGLKIGIMGCIVNGIGEMADADYGYVGAGRGKVSLYKKK
                     ICVEKNIPEEEAVEKLIELIKINGDWTEPIS"
     gene            12997..13461
                     /locus_tag="PLZ54_04510"
     CDS             12997..13461
                     /locus_tag="PLZ54_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66225.1"
                     /translation="MKEKLIVLLLASASFMGLNAQVRGQALGVRLGYGGEVSYQHPLN
                     NQNRIEGDLGFYYDHGFNLAGVYQWVFDLSELSPGFNWYVGLGPQIGSWSNQDNKDYD
                     GLALGIVGQIGIEFNFNIPLQLSLDYRPGWYVLPSSYGGAWEGIALGVRYNF"
     gene            complement(13592..14011)
                     /gene="secG"
                     /locus_tag="PLZ54_04515"
     CDS             complement(13592..14011)
                     /gene="secG"
                     /locus_tag="PLZ54_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOJ66226.1"
                     /translation="MYLLFTILIIIASILLILIVLVQNSKGGGLAAGFSSSNQYMGVR
                     KTADFLEKATWGLIAFIVVLSIGATGIHANQKSGGSDSEIKEQYQKAQEELPGVVAPD
                     FGETQGAGNNQQDNNGGQQNNGNQQKAPQKLPVVPAK"
     gene            complement(14025..14681)
                     /locus_tag="PLZ54_04520"
     CDS             complement(14025..14681)
                     /locus_tag="PLZ54_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005679357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66227.1"
                     /translation="MTQAEFFTLLQHPESITSEHTPDLKDLVEHYPYFSQARMLYLKA
                     LQVSGSIHFEKTIKTAVVYTSQRRWLYHFLYPEVFAKEVYRRPEKGKLTGSYFDLLDV
                     VEKEGGDSGQTLKNLADQLKKARAMVMENPPAPDEEEPIKKKGSTKSSALQSEYFAEE
                     IIPELEISEESVKRLIKNRKYTEALEILKKLNLNNPKKSVYFADQIRFLEKVIENLKI
                     "
     gene            complement(14721..15281)
                     /locus_tag="PLZ54_04525"
     CDS             complement(14721..15281)
                     /locus_tag="PLZ54_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOJ66228.1"
                     /translation="MELNRKPKNSFFGRNTKSVKLLSAIFCGVFLLLASCTVSYKFNG
                     ASIDYTKIKTISIADFPNVSELVYPPLSQQFSEALRDIYTKQTQLRLVRQNGDLNLEG
                     EIVGYQLTPMSISADTYASETKLTVTVKVRFTNKVNPADDFEKTYSAFQVFDSSKMLN
                     DVQEELVKAIITEITENIYNDTVAKW"
     gene            complement(15268..16563)
                     /locus_tag="PLZ54_04530"
     CDS             complement(15268..16563)
                     /locus_tag="PLZ54_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ66229.1"
                     /translation="MQPSEIQAVKQRFGIIGNSELLNRAIDVAVQVAPTDLSVLITGE
                     SGVGKENFPQIIHYYSHRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGAMADRKGY
                     FEVADGGTIFLDEVGELPLSTQVRLLRVLETGEFIKVGSSKIQKTNVRVIAATNLNMT
                     QAVRDGRFREDLYYRLNTVPIYVPPLRERKEDIVLLFRKFAADFAEKYHMPPIKLTED
                     AKKLLTEYYWNGNVRQLKNITEQISVIEQQREITAEILKQYLPKDELERFPVLLSNHR
                     SDQKTFTSEREILYQVLFDMKQDMIELKKLVYNLMNAQGNQELLQKDFPLSPSYVAPH
                     ETFVPATPVPVDVLKPVNNLGREVNQFPNTNKQNSFEEAAEFQEIEDIQEPMTLEDME
                     RQMIKKTLEKHKGKRKAAAEELKISERTLYRKIKEYGIE"
     gene            16902..17645
                     /locus_tag="PLZ54_04535"
     CDS             16902..17645
                     /locus_tag="PLZ54_04535"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HOJ66230.1"
                     /translation="MMEDNFIRLMKAQQKSPADEVINKIKELIESGELKPGDRLPAER
                     KLASDFGFGRTHIREALQKLEFYGIVKTLPQSGTILNGLDINSLNGLLSDVLNLQSYD
                     FYSLVETRCILEMNAIRLCAERRTEEDIEAMEKSHQNFIKFHNDEILRVSYDFAFHRN
                     IAEGSHNPVIKSMLMTIIPDILIIYNKERVCNTDPFVVIKEHAEMINAVKMQNGELAS
                     SLLYQHVQGVMTLARTKLENLKVKSLSMA"
BASE COUNT         5129 a         3680 c         3181 g         5657 t
ORIGIN      
        1 gccttacccg atttttccag cgcatggtct gaatttccta cacttcctac agtggctttg
       61 catgcaccaa gaatttttct gacttctccc gaaggcaatt ttataatgac atatttatct
      121 tcacgggaaa ccaactgagc aaatgtacct gctgaacggg ctattgcggc tccctgaccg
      181 ggacgaagtt ctatattgtg aactatcgtt ccgagcggaa tgttttccaa aggaagtgca
      241 ttacccactt caggtgctac attgctgccc gaaataattt tctgacctac ctgcaaaccg
      301 ttgggagcta aaatgtatga tttttctcca tctgcataat aaagcagtgc gatgcgtgcc
      361 gaacggttgg gatcatattc aatagccttc actgtagccg ggattccatc tttttttctg
      421 gtaaaatcga taactctgaa tttcttttta tgaccaccac caatataacg catggtcatc
      481 ttaccattat tatttcttcc accggtctta cgttttccgt aaaccaggga tttttccggt
      541 tctcttttcg taatagtgtc aaacttgcta ataattttat ttctctgccc cggtgttgtg
      601 ggcaataatt tacgtacagc cattttagtt ttttaaatat tgctaaaaaa atcgatttct
      661 tctccttttt ttacagtaac aatagctttt ttataagccg gtcttttccc tgatattact
      721 ccactctttg tatagcggct ccgttttttg gaaggaacaa tcattgtgtt gacatcaact
      781 accgttacgt tgtacatggt ttcaacagcc tgcttaattt gcagtttatt ggcatccttc
      841 tgtaccttaa agccatagcg attgaacttg tcgccaagct ttgtcaattt ttctgttacc
      901 aatggtttta atatgactcc catttctata ttcctcctta ttctacttta aaaatttgct
      961 ccaaaatagt aaccgatcct tccgtcaata caagagtttt tgcattgagt acattgtaag
     1021 tgtttagttc agaagtcgtt attacattta ttgaaggtaa attacgagcc gacaaatata
     1081 cgtttttatt tgcacttgat aaaacaatta aaggcttttt atcagcaact tgtaaatttt
     1141 tagccaattc gagcattttt tttgttttcg gagcttcaaa ttcaaaattt tcaactacgg
     1201 taattgctcc atttttggct ttataagaaa gagcagattt gcgagccaac tgtttaactt
     1261 ttttattcaa cttgaagctg taatcgcgtg gaaccggacc aaaaacacgt ccgccaccaa
     1321 ctctgagagg agatttaata tcgcccggac gagcaccgcc tccgcctttt tgtcttccca
     1381 atttacgggt gctgcccgaa atttcgctgc gggctttagc tttatgcgtt ccctgacgtc
     1441 ggttggccaa aaattgtttt acatccaaat aaatagcatg gtcgttcggt tcaatgccga
     1501 aaatagcatc attcaacgac acttttctgc cggtgtcttc tcctttgata tttaatacac
     1561 ttaattccat gattatttat tgatgattac gattgaacct ttagctccgg gaacagaacc
     1621 tttgaccagc aataaattat gttccggaat aatttttaat acctgcaaat tttgaacggt
     1681 tactttatcg cctcccatgc gtccacccat tctcatacct ttaaatacac gcgacggaaa
     1741 agaagaagct cctaaagaac cgggcgctct ctgtcggttg tgctgaccgt gagttgtctc
     1801 tccaactccc gaaaatttat gtctttttac aacgccttga tacccttttc cttttgaagt
     1861 acctactaca tcgacaaaag tacttccgtc aaagatttca actgtgataa catcacccaa
     1921 tttaaaatct ccttcaaatc ccttgaactc aaccaagtgt ctttgtggcg ctactcctgc
     1981 aactttaaaa tgtcctgctt ccggcttgtt ggtatgtttt ggtttctttt cttcaaaacc
     2041 tacctgaaca gcttcataac cgtcggtttc aatgctttta atttgagtaa caacacaagg
     2101 accagcttcg ataacagtgc atggaacatt ttttccatca gcactgaaaa cggatgtcat
     2161 tccgattttt ttccctatta atcctggcat ttcaattgtt tgtttttttc tgtctttcgt
     2221 aaacgagaga cagaaagttg atagcttatt ttataattaa atatttttca aatttcctga
     2281 tctttgaggt ttataaccct caaattttgt cagactttaa tttcaacttc aactccgctg
     2341 ggtaattcca acttcatcag tgcatctacc gttttgctgg tggaactgta aatatcgatc
     2401 aaccttttgt aggatgacaa ttcaaactgt tcgcgcgact ttttgttgac aaaggtggaa
     2461 cgatttaccg taaaaattct tttatgggtg ggaagcggaa ttggaccact tactactgca
     2521 cctgttgcct tcaccgtttt tacgattttt tcggctgact tgtcaaccag attgtaatcg
     2581 taagatttta atttaattct aattttttga ctcatcgctg atgattttgt tttagtgtgt
     2641 ttaattttta attaagagtc atttactaat taaaaatgta ttcattaata atgtaataat
     2701 tcaacttttc cttttgcttc agctaccact tcccttgcta acgaattgga gacttcggca
     2761 taatgtgaaa attccattga agaagttgca cgaccggatg ttatggttcg aagcgcggta
     2821 acataaccga acatttctgc caaaggaact tttgccttta taatacgtgc tccggttcgg
     2881 ctcgattcca tgccttcaac ctgtcctctc cgcttattca agtcggcaat tacgtcgccc
     2941 atgctttctt ctggagtaac cacttcaatt ttcatgatag gttccaataa aaccggtttg
     3001 gctttcgtgc aggcattctt aaatgcaatc tgagcacaaa tttcaaacga aagttgatca
     3061 gaatcgacag gatggaacga gccatccaat accgtaacct ttaagcggtc gacagggaat
     3121 cctgctaaaa ctccattttt catagccgac tggaaacctt tctggatgga aggaatatat
     3181 tctttgggaa tatttccgcc ctttaccata tctataaact gcaaacttcc ttcaaaatcg
     3241 ttatcggcag gctccacgcg aacaataata tcggcaaatt taccgcgtcc accggtctgc
     3301 tttttataca cttcgcgtat ttctacaggc tgtgtaatgg cttctttgta ggcaacctgc
     3361 ggccttccct gattgcattc aaccttaaac tcacgtcgca atcggtggat aataatttcc
     3421 aaatgaagtt cacccattcc actaataatg gtctgacctg attgctcatc ggtatgtaca
     3481 gtgaacgttg gatcttcttc tgccaatttt gacaatccta cgccaagctt atcgagatct
     3541 ttttgtgtct ttggttcaat gctgattccg ataactggtt cggggaaatc catcgattcg
     3601 agcacaatgg gatggttttc atcacaaagt gtatctcctg ttttaatatc cttaaatccg
     3661 acaccggctc caatatctcc tgcggaaatc atatctacag gattctgttt gttggaatgc
     3721 atttggaaaa tacgggaaat tctttccttt ttatctgttc gcggattgta tacgtaagaa
     3781 cccgactcaa gttttccaga ataaacacgg aaatagcaca aacgtcccac ataagggtcg
     3841 gtggcaattt tgaaagccaa tgcacacaga ggttcattgg gatcgggatg acgaaccacc
     3901 gttttatctt cgtaacgcgg atcttttccc acaatttctg gattgtcgag tggactcgga
     3961 aggtaagcac aaactgcatc aagcatggtt tgtattccct tatttttaaa tgcagaaccg
     4021 caaataaccg gattaatttc ctttgctaat gttcctttac gaatagctgc cttaatttca
     4081 tcttcagtta ttttagaagg atcatcaaag aatttctcca tcactgcatc atcatattcg
     4141 gctacaattt ccagcatttt ttctcgccat tcgcgggctt cattcaccaa atcggctgga
     4201 atttcttctt ccgaatattc agctcccatt gtttcgtcgt gccagaaaat ggctttcatt
     4261 tttatcaagt ccaccacacc tttgaatttc tcttcagcgc ctatggggat ttgaattggg
     4321 cagggtttag cacccaagac ttctctgatt tggcgaacca cttcaaagaa atcggctccc
     4381 gagcgatcca ttttattgac aaaacataaa cgaggaacat tatatttgtc ggcttgccgc
     4441 caaacggttt ccgattgtgg ttcaacacca ccaacggcac aaaaagttgc aacggctccg
     4501 tcgagaattc gaagcgaacg ttctacttca acagtgaagt caacgtgccc cggagtgtca
     4561 atcaaattta ttttatattg atgctgattg taattccaat aagtagtagt tgcagcggat
     4621 gtaatggtaa tgcctcgttc ctgttcctgt tccatccagt ccatagtggc agcgccatca
     4681 tgaacttctc cgattttatg tgttaaaccg gtataaaaca ggattcgttc ggaggtagtg
     4741 gttttcccgg catcgatgtg agccatgatg ccaatatttc ttgtataact taaatctact
     4801 ttcgccattt taaaaaaatt ccttcaatta aaatttaaag tgtgcaaacg cacgattagc
     4861 ttccgccata cgatgaatat cttctttgcg tttataagct ccaccctgac tgttgaaagc
     4921 atccacgatt tcggcagcca gcttgtcgga catggaatgc cctcctcgtt tacgagcata
     4981 tagaattaaa tttttcatcg aaatggattc tttccgttca ggacggattt ctgtgggaac
     5041 ctggaaagta gctccaccaa cacggcgaga cctaacttcg accaaaggtg ttacattttc
     5101 aagtgctttt ttccagattt caagagagga tttctcttcg ttggggagtt tgttttttac
     5161 attctcgagt gcagaataaa aaatatcgaa agcaatggat tttttgccat cgaacataag
     5221 atggttgaca aattttgtta ccatcggatc attgaaaatc ggatcaggta atacgaccct
     5281 tcttttaggt ttcgattttc tcatttttta ttaaaaattt ccgtttttgt ttttagttgc
     5341 tttcttagta tgcttcaacg acttctttaa gtcatttact caactatcat ctgcttcctg
     5401 aaaaactcaa acaagtgttt aaacttgaat tgattttata tattcaaaaa aatataacct
     5461 tattatttaa aaaacttcac ttctgaagtt taattttatt ttttcttgcc tgttgatgca
     5521 gctgcgggtt gtcctttttt aggacgtttg gctccgtatt ttgaacgacg ctgcagacga
     5581 ccattcactc ccgccgtgtc gagcgtacct ctaacaatgt gataacgaac acccggcaaa
     5641 tcttttacac gacctccgcg aaccagtacg atagagtgtt cctgcaaatt gtgtccctcg
     5701 cccggtatat aggcatttac ttccttctga tttgtcagac gcacacgagc aaccttgcgc
     5761 aaagcggaat ttggtttctt gggcgtagta gtgtaaacac ggatacatac tcctctgcgt
     5821 tgtggacatg aatccaaagc aggtgattta cttttatcaa cgagttctgt ccttcctttt
     5881 ctaactaatt gttgaattgt aggcatttta ttattagttg tataaatatt tttgagttct
     5941 tgatttctta ttcaaacggg ttgcaaagtt aggaattatt tttttattta caatgcaatc
     6001 ctcaatattt ttttaaaaat tcattatctg tttgcttttc tgcctcttaa aagcattcga
     6061 cccgaaaaga atttggctta gtgagtccgg aatttccgca aaaaaacctt cttgcggcaa
     6121 aaaagagaat cggtttttca attgtttata acaaaaacga tgattttaga aatgagaaat
     6181 tatttcagca cgtgaaaaaa ttttttctgt tacattcttt ccggaacttc tatacccagc
     6241 aatgccatac catccttaat cacgtcagca acagccgaag aaagaaccaa tctgaaattg
     6301 cgcaaatcgg tattttcttc tttcaaaatg gaataatcgt gataaaactg attgtattct
     6361 tttactaact cataaaggaa attggctatc agggcaggat taaattcatc acctgcttcc
     6421 ttcaccatta gaggaaattc ggtaagaagt tgaacaagat agcgttcctt ttgagagatg
     6481 gaaaggttca agttattcaa tttaccaaaa gaaattgact gttcttgagc ttttcgcaaa
     6541 accgaacgaa tccgtgcatg ggtatattga atgaaaggcc ctgtatttcc attgaaatct
     6601 atcgattctt ttggattaaa agtcatgttc tttcgtggat ccactttcaa tataaaatat
     6661 tttaaagccc ccaatgctac catacgaacg atattttcaa cttcttcggg agaacaacca
     6721 tcaagttttc ccagttcttc agaagtttca cgcgctgttc gaagcatttc atcaattaaa
     6781 tcatctgcat ctaccaccgt accttcacga cttttcattt ttccgttcgg caattcaacc
     6841 ataccgtagg aaaaatgtac cagacttttt ccccattcaa atcccaactt gtcgagcaaa
     6901 atggaaagaa cctgaaaatg ataattttgt tcattcccta ccacataaac cattttcttg
     6961 ataggaaaat catcataacg aacttttgcc gtgccaatgt cctgagtcat atacactgaa
     7021 gtgccatcag ctctcagcag taatttttcg tccagaccgt catcccttaa atcagcccaa
     7081 accgaaccat cgggacgacg ataaaaaatg cctttgccca agccctcttc aactattttt
     7141 ttcccaacaa gataggtttc ggattcgtaa taaattttat caaaatcaat tcccaattta
     7201 cgataggttt cattaaaacc ttcatagacc caaccattca ttgtttccca aagtttacga
     7261 acttcaggat cgttgttttc ccatgccaaa agcattttct gagcttcctg catcaacgga
     7321 gccgattttt tggcttcctc ttcgctccat ccttcttcga cgagttccct gacttgttgc
     7381 ttatacgcct gatcgaacaa cacataatat ttccccacca gatgatcacc ctttaaacct
     7441 gaactttgag gcgtttcgcc attaccaaac aatttccaag caagcatgga cttacaaatg
     7501 tgaatgcctc gatcgtttac caagtttgtt ttaacaactt tccatccatt ggcttcctga
     7561 atttgagcaa tactccatcc cagcaaattg ttgcgtacat gtcccagatg caagggcttg
     7621 ttggtgttgg gtgaagaata ttcaatcatc atcaagtcgg cattttcttt cagcttaact
     7681 ttccaaaaat ccttctgtag aaatatctca ttcaaccttt cgagccaata tgaaacacta
     7741 attttcagat tcagataacc tttcaccaca ttataatctt caatcacgga tgaactcttc
     7801 aacaaattat cgccaatttc tttccctatc tgttcaggag acttgcgtaa taacttgatg
     7861 aacggaaaaa gcacaaccgt catatcgcct tcaaattcct ttcgcgtttt ctgaacttga
     7921 agaggatttt gaccgatttc caacccatat aattgttgta aagcttgttc aaccagctgt
     7981 tgaatggtgt gttctaaatt catttccaat tgttttgaaa tttggggcac aaagttacaa
     8041 aaaaaaatgc atgttaagat ggcgttagac taatgaagca agataaaaag cttttaccaa
     8101 aatgatgaaa aaaactattt caacatgaga aaaaatgaaa aacaagcaaa cttccattta
     8161 aagatattca aaaagcttac tcaaataaaa aacgcacaaa caaatgcaac aatttgcata
     8221 aatttgtgcg attcctaata aagtaattga aaattaatga attttgtgga gctggaggga
     8281 gttgaaccct cgtccaaaca tggaaacaat atgctttcta catgcttagc ttcgcttggt
     8341 ttttcgtgta caggcaaggc cgaagctacc aacctgtacc ttatcctcta tggttttcat
     8401 tgcatttacg aggctaaatg caactatcct cgattttgct gcacctccgt atcaagctgc
     8461 ctcgaggaga gggcgcttgg aagatgtctc gtccccgcac cttgtgcagg gataaagctt
     8521 aatctactat acttcgatca agcagcgaga gcgtaattag tttcgccagt taattttttg
     8581 agattcggga ttaacgagcc gtaaactcag cgctcgacat gcttacatac cttttctaca
     8641 tgttgtcaaa tccatgcagc cccataattt caaagatcct tacttggcat agcttactgc
     8701 tcttgtttct ctgataacag taattttaac ctgaccggga tatgtcattt catcctgtat
     8761 gcgttttgct atatcgaatg aaagcaattc tgcttcctta tcatctattt tatcggcacc
     8821 gacaatcacc cgcaattctc ttcctgcctg aatggcataa gttttcagaa cgcccggata
     8881 agaaagtgcc aaatcttcca aatccttcaa tcgctttatg taagcttcta caatttcgcg
     8941 acgagctccg ggacgagcgc ccgatatggc atcgcatacc tgaacgatag gagctatgag
     9001 cgattccatt tcgatttcgt cgtgatgggc tccaatggcg ttgcagatat cgggtttttc
     9061 cttgtatttt tcagcaagtt tcatgccaag tattgcatgt ggcaattccg gctcatcgtc
     9121 cggaacttta cctatgtcat gcagcaatcc ggctcttttc gcttttttta cattcaagcc
     9181 caactcggca gccattgttg cacacaaatt ggctgtttct cgtgaatgtt gcagcaaatt
     9241 ttgaccatag gaagaacgat atttcatttt tcccaccaaa cgaatcagtt caggatgaag
     9301 tccatgaatc cccaaatcga tggtggtacg tttccctact tcaataattt cttcttccag
     9361 ctgcttacgc accttgttga ccacttcttc aatgcgtgcc ggatgaatac gtccatcggt
     9421 aaccaactga tgaagagaaa gccgtgcaat ctcgcgtcga acgggatcaa aagccgacag
     9481 cacaatggct tcgggcgtat cgtccacaat gatttcaact ccggtggcag cttcaagtgc
     9541 acgaatattt cttccctcgc gcccaataat gcgacctttg atttcatcag actctatatg
     9601 gaacaccgta acggaatttt cgatggccgt ttctgtagcc aaccgttgaa tgctctgaat
     9661 gacaattctt ttggcttcct tgttggcagt catcttggct tcatccataa tatcgttgac
     9721 ataagccata gcttccgttt tggcttcttc tttcagcgat tcgataagcc gttctttggc
     9781 ctgctcggaa gaaagtccgg aaacagtttc caattgttcc tgagcctgac ggatcaaatg
     9841 ttcgagttcc tttttcttgt tttcgacagt taaaaactga ctttccaagt tctctttcat
     9901 tacatccact tcattcattt ttcgctgcaa ttctccctgt ttttggttga gttgcatttc
     9961 tcgctgttga attcgggtct cggctgcctg aattttggca tttctttgct gtacctgctg
    10021 ttcgtgttcg gctttcagcg caataaactt ttcctttact tcaatcaggt tgttcttttt
    10081 gatcaactcc gcttcggctt cgagttcttt caaagctttt ttatgattgc gtttgaagta
    10141 aatgaaacta ccgcctacac caacaccaaa agatattata atggcaataa ttagattcat
    10201 ttcgtatata tatatttatt tgataaacaa ttaattacaa tttcaatttg ataataaaaa
    10261 aacgcattaa cgttgacaac ttcgggttaa aattttttta accaactaaa caacgtaatg
    10321 cgagaaaaat tgtttgaaga acgattcttc gaaaattact tttcagccaa taattttttc
    10381 agttcctgat tcagagaaga cactttttct gccagaggcg cagtatcctg ctgcatcatt
    10441 tttctgacaa gcattactgc aagctcgtag gcaacaaaaa taaaaacttc ttctgccgac
    10501 cgatgactgt atttcttttg ataaacttcc agatattttc ccaaaacttt ttctgccaat
    10561 cgatacaact cttcttcttt gcgttcaatt ctcaatggca acctgtaacc gcctatctgt
    10621 acatttatta aaaaagtatc gttttcgttc ataaccgctc taatcattta acaaaagagc
    10681 caaacactta tcaatttccc gcaccagttt gttgatttgc tgctttgact tgatccgatc
    10741 ttcatcagaa agggacaaag ttttagcaat tcgcaaatgg tcataatttt tttgaagctc
    10801 ggaaaacttg acttttaaga gttcaatatc ttgttcttta ttgctcaact gcgctcttaa
    10861 caacgaattt tcctcctgca atgacttgaa atcctccatc agctttcgca gattggattc
    10921 aaaagttgcg accaaatcat cataagaatg cgtcatttta tcaaaaaaat ctatacaaaa
    10981 atacattttt cagtcgaaat tcatatacga aattaccttt tttttcgtat attgataata
    11041 attattgttt ttaaattatt tttaataaat gtatttgtgc tctaaattgt ttaaataaca
    11101 ggattcgaga ataaataatc cgtatttttg caaaaaattt cgtaacatca tgtggaaaat
    11161 agatcctttc aattatcagc gtcgccccag caccgaagtc cgcatcggcc aacttttgat
    11221 gggaggcact caaccgatca gaattcaatc gatgaccaat accgacacaa ataacataga
    11281 agcatcgtta gagcaatgca tccgactgat tcgtgccggc agtgaactgg ttcgctttac
    11341 cactcaagga aacagagaag cggaaagttt aaaaaaaatt cacgatttat tgcgtgataa
    11401 agggtatcaa actccgttgt cggccgatgt gcattttaac gccaatgtgg ccgatgtggt
    11461 ggctcaatat gtagaaaaag tccgtatcaa tcccggtaat tatctgggaa gcaacagagg
    11521 aaacattact tccgattata gtgatgaaga atataaagct gaatatgaaa ggataagaaa
    11581 acgctttaaa cagttactga atatttgtaa taaacatcaa acggcgcttc gtattggtgt
    11641 caatcatggt tctttgagcg aaagaattat gaatcggtat ggagatacgg ctgaaggcat
    11701 ggtagaatca tgcatggaat ttcttaggat ttgcagagaa gaaaattttc acaacttggt
    11761 gatttccatg aaagcatcca acacccttac catgattcat tcggttagaa tgctggtgga
    11821 aaggatggaa gctgaaaatt ttcatttccc tttgcatttg ggcgttacgg aagcaggcaa
    11881 tgaagaggaa ggccgaatca aatcggcagt gggaattggc acccttcttt ccgaaggaat
    11941 tggagatacc ataagggttt cgctgagcga agacccggaa gcagaaattc ctgtagctcg
    12001 aaaactgatt tcttatattg acgcaaaaaa aaatcacgaa ccaataattg ccgagaaaaa
    12061 cttccaacat tctcctttca attatcatcg acggacaaca catgccgtag aaaatatagg
    12121 aggaaataat gttcctgtgg ttatagctca cacaaaagaa aattttgaga ttactcccga
    12181 ttattatttc gccgaaaatc agataataaa catccacaat gaagtttttc ccatttttga
    12241 catcgatgaa aaagaaaaaa taataaaaca ttcctcccgc atcaagttcc taaaaatgtc
    12301 gttccccgaa ctgacaaaag aaaatgtgca ttttcttaaa aacacggccg atacagtggt
    12361 tattctcgaa agccatcata aaaatgcacc ggctgaacaa aaagcttttt ttcattcact
    12421 tatgaatgcc ggttgcacag ttcccgtaat cgttacgcaa agctataaag aaaacataga
    12481 ggaagacttg gaaatcaaag ccggggttga ttttggtatg ttatttgttg agggattcgg
    12541 cgatggaatt ttttttaaaa atgaaggaaa catcccgtct gaaaaactta attccatagc
    12601 gtttggaatt ttgcaggcaa caagggcaag aatctcaaaa accgaattta tttcctgtcc
    12661 cggttgcggt agaacaatgt tcgaccttca aaccgttgtt tcccgaataa agtcaaaaac
    12721 ttctcactta aaaggattaa aaatcggcat tatgggttgc attgtcaatg gaataggaga
    12781 aatggccgat gccgattacg gatatgttgg agcagggcga ggtaaagtta gtttgtacaa
    12841 gaaaaagatt tgtgttgaaa aaaatattcc tgaagaagaa gcggtggaaa aattgatcga
    12901 actcattaaa ataaatggcg actggacaga acctatttct taattctgta cttcgcaatt
    12961 tttttctatt tattatcaaa caaaaaactg aaaaatatga aagaaaaatt aattgttttg
    13021 ttacttgctt cggcatcttt tatgggatta aatgcgcagg tcagaggtca agcgctgggt
    13081 gtgagactgg gatacggagg ggaagtttcc tatcagcatc ccttgaacaa tcagaacaga
    13141 attgaaggtg atttgggatt ttactacgat cacggattca atttggcagg agtttatcaa
    13201 tgggtttttg acttgtcgga actttctccc ggttttaact ggtatgttgg ccttggaccg
    13261 caaattggca gttggtcaaa tcaggacaat aaagactatg atggactggc attgggaatt
    13321 gttggacaaa tcggtattga attcaatttc aatattccct tgcaactttc attggattat
    13381 cgtcccggct ggtacgtact gccttccagt tacggaggag catgggaagg tattgcgctg
    13441 ggggtgagat ataatttcta atcaaacgat tcataaaaaa gaaagacaga tgggaaaagt
    13501 ttcctgtctg tcttttttgt ttcatgatcg ataaaaaagc acatcgaata ttcaacgtgc
    13561 tttccaaaat taagttgact attttttcta attattttgc aggtacaaca ggtaattttt
    13621 gaggagcctt ctgctgattt ccgttatttt gctggccgcc attattgtct tgctgattgt
    13681 tacctgctcc ttgagtttca ccaaaatcgg gagctacaac gcccggtaat tcttcctggg
    13741 ctttttgata ctgttctttg atttcggaat cgcttcctcc ggatttctga ttggcgtgaa
    13801 tgccggtggc accaatggaa agcaccacaa tgaaagcaat taatccccat gtagcttttt
    13861 ccagaaaatc ggctgttttg cgaactccca tgtattgatt ggaagaagaa aaacccgctg
    13921 ccagtccacc tccttttgaa ttttgtacca acacaataag tatcaggaga atggaggcaa
    13981 ttataattaa aatagtaaac aaaaggtaca taaaagtata ttatttaaat tttcaagttt
    14041 tctattactt tttccaagaa acgaatttgg tctgcaaagt aaacactttt tttcggatta
    14101 ttcaaattta atttttttaa aatttccaat gcttcagtgt attttctgtt cttaattaac
    14161 cttttaaccg attcttctga aatttcaagc tcaggaatga tttcttccgc aaaatattcc
    14221 gattggagtg cggacgattt tgtagaccct ttcttcttaa tgggttcttc ttcatctggt
    14281 gccggtggat tttccatcac catagctcgc gcttttttca actgatcggc caaatttttc
    14341 aaagtctgac ctgaatctcc tccttccttt tcaactacat ccaataagtc gaaataagaa
    14401 cctgtaagtt ttcctttttc gggacgacga taaacttcct ttgcaaaaac ttccggataa
    14461 agaaaatgat agagccagcg acgttgagaa gtatagacaa ctgcagtttt tatggttttt
    14521 tcaaaatgaa tactacccga cacttgaaga gctttcaggt aaagcatcct tgcctgcgaa
    14581 aaatagggat aatgctccac caaatctttt agatcaggag tatgttccga tgtaatgctt
    14641 tcgggatgct gcaaaagtgt aaaaaattcc gcctgtgtca ttattcctaa atgctcaaaa
    14701 aaaccagaaa aaacatttcc ttaccattta gcaacagtgt cgttataaat attttccgta
    14761 atctcggtaa taatcgcctt caccagctct tcctgcacat cattcaacat ttttgagctg
    14821 tcgaaaacct gaaaagccga ataggttttt tcaaaatcat ctgccggatt caccttattg
    14881 gtaaaacgaa ccttaacggt taccgtcagt ttggtttcgg aagcataggt atcggcactg
    14941 atggacatag gcgtaagctg atagccgaca atctcgcctt ccaaattcaa atctccattt
    15001 tgacggacaa gccgaagttg ggtctgcttg gtgtaaatat cgcggagtgc ttcggaaaat
    15061 tgctgcgaca aaggcggata aaccaactcc gaaacatttg gaaagtcggc aatggaaata
    15121 gttttgattt tagtgtaatc gatagaagct ccattgaatt tgtatgaaac ggtacaggaa
    15181 gccaaaagaa gaaaaactcc acaaaaaatg gccgataata gtttgaccga tttcgtgttt
    15241 cttccaaaaa atgagttttt cggttttcta ttcaattcca tattctttta ttttgcggta
    15301 cagagttcgt tctgaaattt tcaattcttc agcagctgct tttcttttgc ctttatgctt
    15361 ttcgagcgtc ttctttatca tttggcgttc catatcttcg agcgtcattg gttcttgtat
    15421 atcttcaatc tcttggaatt cagcagcttc ttcaaaagaa ttttgtttgt ttgtattggg
    15481 gaactgattt acttcgcgcc ccaaattatt gacaggtttc aacacgtcca ccggcaccgg
    15541 tgtagccgga acaaaagtct catgcggagc aacataagaa ggcgataaag ggaagtcttt
    15601 ttgtagcaat tcctgattgc cctgagcatt cattaaattg taaaccagtt ttttcagttc
    15661 tatcatatcc tgtttcatgt cgaaaagcac ctgatagagg atttctctct cggaagtgaa
    15721 agttttctga tcgcttcggt gattggacaa caaaacagga aaacgctcca attcatcctt
    15781 cggcagatat tgtttcaaaa tttctgctgt aatttcccgt tgctgctcaa tgacagaaat
    15841 ttgttcggta atatttttaa gttgccgaac attgccattc caataatatt cggtgagcag
    15901 tttttttgca tcttccgtaa gtttaatggg tggcatgtgg tatttttcgg caaaatcggc
    15961 tgcaaatttt ctaaaaagca gaacaatatc ttctttcctt tccctcaaag gcggaacata
    16021 aataggaaca gtattcaaac gataatacaa atcttcccga aaacgtccgt ccctaactgc
    16081 ctgcgtcatg ttcaaattgg tggcagcaat aacacgaaca ttggttttct gaatttttga
    16141 agaaccaacc ttgataaatt cacctgtttc gagcacacgc aataagcgaa cctgagtgga
    16201 aagcggtaac tcccccactt catccaaaaa aatagtacct ccgtctgcta cttcaaaata
    16261 gccctttcgg tcggccatag ctccggtgaa tgaacctttt tcgtgcccaa acagttccga
    16321 atcaatcgtt ccttcaggta tggcgccaca attgacggca atataaggtc cgtgtttgcg
    16381 atgactgtaa taatgaataa tttgaggaaa attttccttg ccaacaccac tttcgccggt
    16441 aatcaatacc gataaatccg ttggtgccac ctgaacggcc acatcgatag cccgatttaa
    16501 caattcagaa tttccaataa ttccaaaacg ttgttttaca gcttgtattt ctgatggttg
    16561 catgtttcta acaataattt actgcaataa tgacataatt gtctgacaaa attaatgaaa
    16621 ataaaaggat tttgtttatt ccacatcatg aaaaagtcta ataaaagaat ttatttccca
    16681 atttttgatt accgaatttg aaaaatgttg ttttttggaa ttgagaaaaa gaaatttaaa
    16741 acgaagaaaa caaattattg gaaaaaattt tgaaggtaaa aaaatttgtg taattttggt
    16801 caaccaatta aacgggttaa aaaataattt taaaattttt cctttttttc ggcagagaaa
    16861 tattcggaac aaataagtca atagaaaaaa ctacaaatat tatgatggaa gataatttta
    16921 tccgattgat gaaagcccag caaaaatcgc ctgcagatga agtaatcaat aaaattaaag
    16981 aattaattga gtcaggtgaa ttaaaacccg gagaccgatt gcctgccgaa aggaaattgg
    17041 caagcgactt tggttttggg cggacacaca ttcgcgaagc attacaaaaa ctcgaatttt
    17101 atggcatcgt aaaaacactt ccacagagtg gaactattct caatgggctg gatataaatt
    17161 cattaaacgg cttactttct gatgttttaa atcttcagag ttacgatttt tattcgttgg
    17221 ttgaaacaag atgtatcctc gaaatgaatg ccattcggct atgtgccgaa agacgtaccg
    17281 aagaagacat tgaggcaatg gaaaaatctc atcagaattt tatcaaattc cataatgatg
    17341 aaattttacg tgtcagttat gactttgctt ttcaccgcaa catagcagaa ggcagtcaca
    17401 atccggtcat caaatccatg cttatgacca ttattccgga tattctcatc atctacaata
    17461 aagaaagggt atgtaatacc gacccttttg tagtaattaa agaacacgcc gaaatgatca
    17521 atgcagtgaa aatgcagaat ggagaattgg cctcatcctt gctttaccaa catgtgcaag
    17581 gcgttatgac tctcgctcgc acgaaacttg aaaacttaaa agtgaaaagt ttgagtatgg
    17641 cttgaga
//
LOCUS       DAOSCH010000039        17279 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2275_length_17279_cov_5.668718, whole genome shotgun sequence.
ACCESSION   DAOSCH010000039 DAOSCH010000000
VERSION     DAOSCH010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17279
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2275_length_17279_cov_5.668718"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="PLZ54_04540"
     CDS             <1..905
                     /locus_tag="PLZ54_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOJ66231.1"
                     /translation="HYSTFIYLRKLNDTEKINTFFSGINEKLPDDGNFVCCFEKKSTR
                     KKKFLQKYPPGINYILYSLDYFFKRFIPKLFFSRRIYHSLKGGKNRILSKAEVLGRFY
                     CHGFEVIEEKKIDGLLYVWAQRKRNPTPVYNRAYGPLIKLKRYGKNGKEIYVYKFRTM
                     HPYSEYLQEYVYQRHSLQDGGKFNKDFRVTTIGKFMRKYFLDELPMLINIFRGEMKLI
                     GIRPLSQQYLNLYNDEIQQLRMKHRPGLLPPFYADMPRTLDEIQESERKYLLACEKNG
                     VLWTDIKYFFLILKNILFHHARSA"
     gene            1087..2814
                     /locus_tag="PLZ54_04545"
     CDS             1087..2814
                     /locus_tag="PLZ54_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66232.1"
                     /translation="MKKHQKKIHIALLLFSNILFVVAQDVPEHISYSRIYDFLDELAN
                     EKLIEINSVVKPYPRTFIAQLLQQAKSKEAKLNRRQQKELYFFLENYALEQNRLPQYD
                     YPLFEQTNWQLDVFPPSVHYRDSLLRGRVMPILGMNILYNNNDKITQRWFGIDFQSMI
                     GKHFAVYASLRDVSQQGELLAESTYLTPYQGGAYKRTTNGGDYSEMRGGISYAWEWGS
                     VALTKDHVQWGDNYHGSNIIAGRTPSFPMLKLSLKPAKWLEMNYFHGWLTSNVIDSTR
                     YYLSNQNEKMYRYTNKFLAANMISFIPLPQLHLSAGNAIVYAESNVQPAYLIPLAFYK
                     SMDHTLTMGTENQNSQLFLNISSRNIQHLHLFTSIFVDEFSISRIKPSSKEKNPISYK
                     LGFRLSNFPLENLSLRGEYTKNAILSYKHSIPALTWATNGFNLGNYLGDNSQEIYAAL
                     QYKPLRGLDINLSWTQAEHGNEYAYIRHGTWQGSKASVLDIISQPFMKDKIWSNKTLA
                     LHVKYELFPNTYATLNIENSYIQTFNAVSEVTFGERRMTAGEALNYYTPKFLQGKQTT
                     FTAGFSFGF"
     gene            complement(2876..3799)
                     /locus_tag="PLZ54_04550"
     CDS             complement(2876..3799)
                     /locus_tag="PLZ54_04550"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HOJ66233.1"
                     /translation="MKISNLLVILGLLFCFSFVQAKSIQKITRTYAVKDSLELKMDIY
                     TVDSTSSSLQPCLVFVFGGGFKEGKRDEPTYQPYFDYFTKKGFMVVSIDYRLGMKDQR
                     SPGVFNTKPLQNAISLAVLDLYSATKFLIEKADSLKIDKNKIIISGSSAGAITVLQAE
                     YELKNHHAITSELPHDFRFAGVISFAGGIFSTKGMPSYAESPAPTLFFHGSADKLVPY
                     KETRLFNKGMFGSRSLTNKFHKKKYSYLFYSMEGLGHEVASFPMKEFLPEIEQFIQNF
                     VFEQKPWMVEVYFTDKERKTEKSVTPADYYK"
     gene            4240..6720
                     /locus_tag="PLZ54_04555"
     CDS             4240..6720
                     /locus_tag="PLZ54_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ66234.1"
                     /translation="MHSRKLFLFVFFSILSISLFSQNKVRIYGYVIDTNNRGIELANV
                     YVQHTNIGTSTNQNGYYELTVDIKDSIKLVFSMIGYQTVTYTVHPKLKVIQLTVELPP
                     LSKQIEDVNVIGQRRQTSTIDVVDPSKYRLMPNSAGGIESLLISFTGVSSSNELSSQY
                     NVRGGNFDENIVYVNGIEVYRPLLIRAGQQEGLSFINPDMVQNVSFSSGGFNAEYGDK
                     MSSVLDISYKKPSAFEATASASLLGASAYMGTAGKRFSQMHGIRYKTSKYLLGTMDTQ
                     GEYDPSFVDYQTFLTYQIKPKWELTFLGNFSQNDYRFIPQTRETSFGTYSMGRKLTIF
                     FEGQEKDLFQTSFGALTLNFRPSKSTKLSLIGSAFYTNERENYDISGEYILSEVKMDV
                     DPDKQTGESLGIGNYHEHARNKLNATVANLSHLGEYAKNGHLLKWGANVQIEKIDDQI
                     NEWEWRDSAGYSLPYNNRQVNLYYNMNASNSLKTLRTTSFLQDTYKYSSTAGTFSFTG
                     GLRSHYWTFNREFLLSPRFTVAYLPHWEKDFSFRFATGLYYQAPFYKEIRDTLTDALG
                     NVDIKLNSNIKAQRSLHFVLGGDHYFRAWGRPFKFTTEAYLKLADRVVSYSVDNVRIR
                     YSGENDAKAYTAGIDFKLFGELVPGTDSWINLSLMDSKEDIIGDSYVRHTYDDEGNIL
                     TSETVYPSWVPRPTEQRYAFSMMFQDYLPSNPKYKLQLKLIWSDGLPFGAPRNEQFRS
                     AFRMPPYRRVDIGGSRLLVNGSDEWMNKSWLGHVKNIWINVEVFNLLDFKNVNSYYWV
                     TDIYQHQVAVPNYLTGRQLNLRLLVDFK"
     gene            complement(6934..9627)
                     /locus_tag="PLZ54_04560"
     CDS             complement(6934..9627)
                     /locus_tag="PLZ54_04560"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOJ66235.1"
                     /translation="MKIHFKIPYYTHWGQQIFVCGNLPELGNNVPEKSFQLHFQNTED
                     WYGEIEIHQSEHFELEYRYLLYEENTKRFTYEWIEDRKITIDPRMEHIFCFDFWNAPG
                     AVENVFFTAPFKKVFFSDNFNHSQKTRSKKNFTHIFRIKAPLLKNHQVPCLIGDCKVL
                     GSWNTVSPLLLTRDGENWWKAEVDLGKEKSDVHYKYGIYDTENGQFLMFENGFDRTAP
                     VFNAKNTFVRLSDGFLRMNSQSWKGAGVSVPVFSLRTKESFGVGDFGDIRLLVDWAEK
                     AGLKLIQVLPLNDTIGTHTDADILPYAAISAFALNPLYLNLPSLGKLPENHPLQKEYE
                     ERQPQLNALEWIDFLEVVNFKLKYVKALFSFHQEEFLQNKDFLTFFENNKHWLIPYAA
                     FCVLRDRYGTPDYHQWKEFSTFNAEKIAAFVSANQPHYADIALNYFIQYHLHIQLMEA
                     ASYAHQHGIVLKGDIPIGVNKNSVETWAHPELFHIDMQAGAPPDMFSIKGQNWELPTY
                     NWEAIKQTGFDWWKKRLKQMSNYFDAFRIDHILGFFRIWEIPNDQLEGIMGHLYPSIP
                     VYLDEFQAKGIWFDYNRFCKPFINDQVLEQLFGTDAGWVKVNCLQIEDGWILRLKPFY
                     RSQKNVQQLYEEGQFSENIRNGLFDLISNVLFFEVEGSHGTRFYPRWGMHSLSTFQYL
                     DDFTKQKLDEIYIDYFYHRQDEFWFQSGMEKLPVLKQTTDMLICGEDLGMMTPCVTRA
                     MKELGILSLEIQRAPKTNKIEFFHPSDAPYLSVVTPSTHDMSTIRGWWEEDRNITQRF
                     YNTQLGHWGEAPYFCEWWICRDILLQHVYSPAIWAIFQIQDLLGVSEKLRRENPHEER
                     INVPSDSKKSWRYRLHLNLEDLLKEDDFNRQIRNYIRQAGR"
     gene            9921..10865
                     /locus_tag="PLZ54_04565"
     CDS             9921..10865
                     /locus_tag="PLZ54_04565"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOJ66236.1"
                     /translation="MAASNSSFKVFSGTNSRSLAEKICQSLGCPLGKMNIEHFADGEF
                     AVSYEESIRGLNVYLVQSTFPNSDNLMELLLMIDAAKRASAYKVIAVIPYFGWARQDR
                     KDKPRVSLGAKLIADMLSVAGIDRLITMDLHADQIQGFFNVPVDHLYASTIFAPYIQT
                     LRLKNLVIAAPDVGASKRAGSYSKYFDVPMVLCHKTREKANVVGEMRIIGDVKGKDVV
                     IVDDMVDTAGTLCMAANLMMEQGANTVRAIVSHGVMSGSASEKVEKSALTELAFTDSI
                     PFDASKCSKVKILSIADMFADTICRVQEHKSISEQYIF"
     gene            complement(10989..12458)
                     /gene="crtI"
                     /locus_tag="PLZ54_04570"
     CDS             complement(10989..12458)
                     /gene="crtI"
                     /locus_tag="PLZ54_04570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HOJ66237.1"
                     /translation="MKEKVIIVGAGLGGLATGLRLAKEGYEVEFLEKDTKAGGRLNQI
                     KKDGFTFDTGPSFFSMSYIFKEFAEKCGINLPFEFIELDPLYSVNFSGEDKTYFLYKD
                     IHKLAKQFDDVEPDFELKMEKYLQKSAQLFNDTVDIVIKRNFDSYLDYFLALMKVNPV
                     HLPILFKTFWQHVCDYFTSKQAREIISLVAFFLGRTPFDTMGIYSLLSHVEFRHDGYY
                     NVKGGMYKIVEGMLEELSKLNVKFHYETEIVAYHAENRKIVSLKDAQGNLWKADKFVI
                     NADAALFRSKVMKYKNFSEKKLDKMEWTMGYLTFYLGLKCKLPQVNHHNYFLGSNYEQ
                     YAGNILQNPDTLEKPYYYVNVVSKYNPECAPEGCESLFFVCPVPNLKYKNDWSDREKI
                     VNSIIDDFSQRIKKDISTEIISKTIFTPQEWRDKFSLYQGSGLGLSHKFTQIGAFRPK
                     NYDEKHDNLFYVGASTIPGAGLPMAVISAELVVERILGK"
     gene            complement(12462..12893)
                     /locus_tag="PLZ54_04575"
     CDS             complement(12462..12893)
                     /locus_tag="PLZ54_04575"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66238.1"
                     /translation="MIEAYKSAFYGFQIGMNKLKAPVLGPKSVEAAKLAIQYDPNNYF
                     GYLQMGNIYYYSPAAFGGSKAKAIESYRQAEKLILSNNHRLKNDWNYLSLLVTLAQAY
                     ENTGNDESAKIYYQKILNVEPDFAWVKNKLYPEFLKKTQKK"
     gene            complement(12886..13206)
                     /locus_tag="PLZ54_04580"
     CDS             complement(12886..13206)
                     /locus_tag="PLZ54_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66239.1"
                     /translation="MERKKSLVLFFLLILLHLHVHASHRENIYKAYINNDMQAWKNTI
                     DKMNLSSGNASDEFLLELVNYEYGYIGWCIGNKKTAEAKEYLKKAENRLITLEKKSIS
                     LIND"
     gene            complement(13185..14276)
                     /locus_tag="PLZ54_04585"
     CDS             complement(13185..14276)
                     /locus_tag="PLZ54_04585"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ66240.1"
                     /translation="MIYLAWVVFGFTFFQLCIAGLNYFFREKIPISTNRISDEKISVL
                     IPARNEEQHIGNLLNDLSGEDDNILEIIVFDDLSSDHTVEIVNQFIGTNEKIRLIRST
                     HLPAGWLGKNFACYSLANAAKGNYFLFLDADVRMEKEIVVSSVKFFKQKSLHLLSIFP
                     TQLMMTKGEKMTVPNMNYILLTLLLLPLVRKSSFPSISAANGQFMLFDADTYKKFQPH
                     EKFKDKRVEDIEIARFYKKLKLKIACLTGIKQVRCRMYENFAEAVNGFSKNVIHFFGN
                     SFLIAVLFWIVTTFGWLAVWLAFGLPTLAVYLLAVVCIRIFFSLASNQSILDNLIFFI
                     PQQFSLGIFIYQALINRSKNQYLWKGRKV"
     gene            complement(14280..14906)
                     /locus_tag="PLZ54_04590"
     CDS             complement(14280..14906)
                     /locus_tag="PLZ54_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ66241.1"
                     /translation="MLKAKHHCLIYPFFRRYTLLLMKRNFHSVNVIRTNFSRNENLPV
                     LMIANHISWWDGFWAMYLNLKVFHKKFYFMMLEDQLLKFRFFQWTGGFSIKKKSREMI
                     ESLQYAAKILDNPDNLLLIFPEGKLQSLYNPLHVFEKGIERIVEGHKNNFQLVFVANF
                     IDYLSHKKPDLYMYVSEYQGNVFSHSKLQEAYNVFYGNCLDIQKSKTE"
     gene            complement(14916..15581)
                     /locus_tag="PLZ54_04595"
     CDS             complement(14916..15581)
                     /locus_tag="PLZ54_04595"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HOJ66242.1"
                     /translation="MQISLLLEKFKQKYPERKVRQFIFTFYAIGIVGLVLPATHVFFV
                     YLIPLALLLSAFILLPFQEDDFSTKTGVIFTVIFVVSFTVEAIGVNTGKIFGQYFYEN
                     GLGVKCFGTPLIIGLNWALLVYLTASIFQSFRINKFFLVLFPSVLMVLYDVVLEQVAP
                     KMQMWFWNADRVPFRNYLVWFLMALIFHTLLVISGLKLKNPLSKIVFSAQFLFFLILM
                     FIL"
     gene            complement(15572..16126)
                     /gene="idi"
                     /locus_tag="PLZ54_04600"
     CDS             complement(15572..16126)
                     /gene="idi"
                     /locus_tag="PLZ54_04600"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOJ66243.1"
                     /translation="METNKENFVQLVDENDQPVGIMEKYEAHKKAALHRAISVFIVNS
                     KGDWLIHRGALDKYHSNGLWTNATCSHPYPDESYLEAAKRRLKYEMGINCELIPLFHF
                     IYQEKVNDELTEHELDHVFFGVSDDVPIHNTGEVEEWKYIPYRQLLEDVENHPEKYTV
                     WFKIIWKKVKAYIDTENLTESSCR"
     gene            complement(16113..16964)
                     /locus_tag="PLZ54_04605"
     CDS             complement(16113..16964)
                     /locus_tag="PLZ54_04605"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004360351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOJ66244.1"
                     /translation="MDKKYNALSFSTSKFVTKFYSTSFYSASQYFSKEIQEAIFSIYG
                     FVRLADEIVDSFHFSDKEYLLRKFESDYYDAVKNGISLNPILHSFQMTVGKYGISDEY
                     VQHFLSSMKADLEKKEYKSQIELNTYVYGSADVVGLMCLKVFCKGDEELFDRLMLPAT
                     KLGSAFQKVNFLRDIKQDTQELGRHYFPQIVEEGLTEQTKKELVEDIRKDFDDAYKGI
                     VLLPADSRLGVLIAYKYYTALLRKIEKTAACDILNKRIRISDAGKMFLVLSTQIKYYL
                     RAFYGNK"
BASE COUNT         5398 a         3393 c         3126 g         5362 t
ORIGIN      
        1 atcattattc aacttttatc tacctgcgta agttgaacga tacggaaaaa atcaacactt
       61 ttttttcggg tatcaacgaa aaattgccag acgatggaaa ttttgtttgt tgttttgaaa
      121 agaaaagcac cagaaaaaag aaattccttc aaaaatatcc tccgggtatc aattatattt
      181 tatattcttt agattatttt ttcaagcggt ttattccaaa acttttcttt tcgaggcgca
      241 tttatcattc gctgaaaggt ggaaaaaacc gtatcctttc caaagcagaa gtattgggga
      301 gattttattg tcacggtttt gaggtgattg aagagaaaaa aatagatgga cttctgtacg
      361 tatgggcgca aaggaaacga aatccgacac ccgtttacaa tcgtgcctat ggcccattga
      421 ttaaattgaa acgctatggc aaaaacggaa aggaaatcta tgtttataaa tttcgcacca
      481 tgcatcccta ttcggaatat cttcaggaat atgtttatca gcggcattct ttacaagatg
      541 gaggaaaatt caacaaagat tttcgggtaa ccaccatcgg aaaattcatg cgcaaatact
      601 ttctggacga attgcccatg ctgatcaata ttttcagggg tgaaatgaaa ttgattggca
      661 ttcgcccact tagccagcaa taccttaacc tgtacaatga cgaaattcaa caacttcgca
      721 tgaaacaccg acccggattg ctaccccctt tctatgccga tatgcctcgc acgttggatg
      781 aaattcaaga atcggaacga aaatatttac tggcctgcga aaaaaatggc gttttatgga
      841 ccgatataaa atatttcttt ctcattttga agaatattct gtttcatcat gcacgaagtg
      901 cctgaaaatt caagaatcca agaacttata aaaagcaaaa gtataaggaa ccaaaataag
      961 caacaggtta aatcaaactt ttttcaaaaa aatttcaaga cttctttagc gaaaaaattt
     1021 tttaataaca acatattgcc tttattttca actaattttg aaaaatctta aaaatttggc
     1081 ttcaacatga aaaaacatca aaaaaaaata catatcgcac tgctgctctt ttccaatatc
     1141 ctttttgttg ttgctcaaga tgttcccgaa catatttcct actcccgcat ttacgatttt
     1201 ctggatgagc tcgccaacga aaaacttatt gaaatcaact cggttgttaa gccctatcca
     1261 agaactttta tagcccaatt gttgcaacag gcaaaatcaa aggaagcaaa actcaaccgc
     1321 cgtcaacaga aagaattgta tttttttctg gaaaattatg cgttggaaca aaatcgcctc
     1381 ccgcaatacg attatccgct tttcgaacaa accaactggc agttggatgt tttccctcct
     1441 tccgttcact accgcgattc actgttgcgt ggacgtgtga tgcccatttt gggaatgaat
     1501 attctctaca acaacaacga caaaattacg caacgctggt ttggaattga ttttcaatcg
     1561 atgataggaa aacattttgc cgtctatgct tccctccggg atgtttcgca acagggagaa
     1621 ttgcttgccg aatcaactta cctgacaccg tatcagggcg gagcctacaa acgtactacc
     1681 aatggtggcg attacagcga aatgcgtggc ggtatttcgt atgcttggga atggggaagt
     1741 gttgcactga caaaagacca tgtgcaatgg ggcgacaact atcacggttc caacattatt
     1801 gcgggtcgca cgccttcgtt tcccatgctg aagctgagcc tgaaaccggc aaaatggctg
     1861 gaaatgaatt attttcatgg gtggctgact tccaatgtaa tcgacagcac acgttattat
     1921 ctttccaatc aaaatgaaaa aatgtatcgt tacaccaata aatttctggc tgccaatatg
     1981 atatcattta ttcccctgcc ccaattgcat ctgtcggcgg gaaatgccat tgtgtatgcc
     2041 gaaagcaatg tacagcccgc ttatttgatt cctttagctt tttacaaatc gatggatcat
     2101 acgctgacta tgggtaccga aaatcagaat tcgcaactgt ttcttaatat cagttcaaga
     2161 aacatacaac atttgcactt gtttacttcc atttttgtgg acgaattctc catttcccgc
     2221 attaaaccct ccagcaagga aaaaaatcct atttcctata aactgggatt tcgccttagc
     2281 aatttcccgc tggaaaatct ttcgttgagg ggtgaataca caaaaaatgc catccttagt
     2341 tacaaacatt ctattcctgc tctgacatgg gcaaccaatg gtttcaatct gggaaattac
     2401 ttgggcgaca attcgcagga aatttatgcc gccttgcaat acaaaccttt gcgcgggctg
     2461 gacataaacc tgtcgtggac acaagccgaa cacggaaatg aatatgccta tattcgacac
     2521 ggaacctggc aaggctcaaa agcaagtgtt ctcgacatta tcagtcaacc gtttatgaaa
     2581 gacaaaattt ggagcaataa aactctggca ctccatgtaa aatatgagct tttccccaat
     2641 acttatgcaa cattgaacat agaaaacagc tatatacaaa cttttaatgc tgtttcggaa
     2701 gttacttttg gtgaaaggag aatgactgcc ggagaagcat tgaattacta tacgccaaaa
     2761 tttcttcagg gtaaacaaac tacttttact gccggcttta gctttggatt ttgaacaaaa
     2821 gttttcttaa aatgatgaaa gtataaatag cgaaaaacgt ttttcggcaa actgattact
     2881 tgtaataatc ggccggagta actgattttt ctgtttttcg ctccttgtcg gtaaaataaa
     2941 cttcaaccat ccagggtttt tgttcaaaca caaaattttg aatgaattgc tcaatttcag
     3001 gtaaaaattc tttcattgga aaacttgcaa cttcatgtcc taaaccttcc atgctgtaaa
     3061 aaagataaga atatttcttt ttatgaaact tgttggtcaa cgatctggaa ccaaacatgc
     3121 ccttgttaaa aagtcgagtt tccttatatg gcaccagttt gtcggcacta ccatgaaaaa
     3181 acagggttgg tgcaggtgat tcagcgtaag acggcatgcc tttcgtgctg aaaatgcccc
     3241 ctgcaaagga aattacgcct gcaaaacgaa aatcgtgagg taattcggat gttatagcat
     3301 gatgattctt taattcgtac tcagcctgaa gaactgtaat ggcaccggca cttgaaccgc
     3361 taataatgat cttatttttg tcaattttca atgaatcagc cttttctatt aaaaattttg
     3421 tggcagaata taaatctaaa acagctaaag aaatggcatt ttgcaaaggt ttggtattaa
     3481 aaaccccggg tgagcgttgg tctttcatcc ccaatcgata gtcaattgaa accaccataa
     3541 aacctttttt tgtaaaataa tcaaaataag gttggtaagt cggttcgtct cttttccctt
     3601 ctttaaaccc tccaccaaat acaaatacca aacatggttg caaagaagaa gatgtggaat
     3661 cgacagtata aatatccatt ttcagttcca aagaatcctt aacggcataa gtacgtgtta
     3721 ttttttgaat gcttttggct tgtacaaaag aaaaacaaaa taataagcct aaaattacta
     3781 ataagttact gattttcata tgtataaatt tatttgtcgt aaagtcaact gaataatgca
     3841 actttttagg aaaagaaaag gtaaagaaaa attagcgcaa aacgagcaac aaaatccttg
     3901 ctgctttgta aagtatatat tttaccttgc cacttttccc ctaaaaaatt atcaaaatga
     3961 agcatagaac tttagaacaa aagtacgaaa tttgtctcac taaaaatttc ctttataagt
     4021 taaattcagc ttataaatta atatctttgt gggcaacata cttttatagt ctgttttgta
     4081 tgatcaaaaa gctacaaatt agaggaatga tccaaattcg gttagctttt ctttattcta
     4141 ccaatgcttt ctatttgttt tttggtcaaa aatccaacaa aataaacttt tgtttccgac
     4201 gtttaaaaaa gtattaattt tagtgtgaag acaataacaa tgcacagccg taaattattt
     4261 ttgtttgttt tcttttcaat actatccatt tcgctttttt cgcaaaataa agtgcgtatt
     4321 tacggttatg taatcgatac caacaatcgc ggcatagaat tggcaaatgt atatgttcaa
     4381 cataccaaca ttggcacttc taccaatcag aatggctatt atgaactaac ggtcgacatc
     4441 aaggattcga taaagctcgt tttttctatg attggttatc aaacggttac ctataccgtt
     4501 catcctaagt tgaaggtgat acagttaact gtggaacttc ctccgctatc gaaacaaatt
     4561 gaagatgtga atgtaatagg ccagcgacgg cagacatcta ccattgatgt ggtcgatcct
     4621 tccaaatatc gcttgatgcc caattcggcc ggcggaatag aatcgctgtt gatttctttt
     4681 acaggcgtaa gttccagtaa cgaactgagt tcgcaataca atgtaagagg cggaaatttt
     4741 gacgaaaata ttgtgtatgt taacgggatt gaagtttatc gccctttgct gattagagcc
     4801 ggacaacagg aaggtttgag ttttatcaat cccgatatgg tacaaaatgt atccttttcg
     4861 tcaggtggtt tcaatgccga atatggtgat aaaatgtctt ccgtgttgga tatcagctat
     4921 aaaaaacctt ctgcatttga agcaacagcc tctgccagtc tgctcggggc ttcggcttat
     4981 atgggtacgg ccggcaagcg gttttcacaa atgcacggca ttcgttacaa aacttccaag
     5041 tatttgctcg gaacaatgga tactcaggga gaatatgatc cttcttttgt ggattatcag
     5101 acttttctta cctatcaaat aaaacccaaa tgggaattga catttctggg gaatttttca
     5161 caaaatgact accgttttat tcctcagact cgcgaaacat catttggcac ctattcgatg
     5221 ggacgaaaac tgaccatttt ttttgaagga caggaaaaag acctttttca aacttctttc
     5281 ggtgcgttga cgttgaattt tagaccttca aaatcgacaa aactaagtct catcggttcg
     5341 gcattttaca ccaacgaaag agagaattac gacatctcgg gcgaatatat tttgagtgaa
     5401 gttaaaatgg atgttgaccc cgataaacaa accggcgaat ctttgggtat tggaaattat
     5461 cacgaacacg cccgaaacaa gctgaatgct acggttgcca atctgtcaca tttgggcgaa
     5521 tacgcaaaaa atggtcattt actgaagtgg ggtgccaacg ttcagataga aaaaatcgat
     5581 gaccagatca atgaatggga atggcgcgac tcggccggct attctttgcc ttacaataac
     5641 aggcaagtga atttgtatta caacatgaat gcttccaatt cgctgaaaac gcttagaaca
     5701 acgtcctttt tacaggatac ctataaatat tcttccactg ccggaacttt ttcatttacc
     5761 ggcggacttc gttctcatta ttggactttc aaccgcgaat ttttactcag tccgcgtttt
     5821 actgttgcct atcttccgca ttgggagaaa gatttcagtt ttcgttttgc tacggggctt
     5881 tattatcagg ctccttttta caaggaaatt cgcgatacgc ttaccgatgc tttgggaaat
     5941 gtggatatca aattgaattc caacataaag gcacaacgtt cgttgcattt tgtgttgggt
     6001 ggcgaccact atttcagggc gtggggaaga cctttcaaat tcactaccga agcttatctt
     6061 aagttggcag acagggttgt ttcctattcg gtggataatg ttcgcattcg ctattcgggt
     6121 gaaaatgacg ccaaagctta tactgccggc atcgatttca aacttttcgg agaacttgtt
     6181 cccggaaccg attcgtggat caatctgtcg ctgatggatt cgaaggaaga tattattggc
     6241 gacagctatg tgcgtcatac ttacgacgac gaaggcaata ttttgacttc cgaaacggta
     6301 tatccatcct gggtgcctcg tcctacggag caacgctatg ctttttcgat gatgtttcag
     6361 gattatttgc cctcaaatcc aaaatacaaa ttacagctca agttaatttg gtctgacgga
     6421 ttaccgtttg gcgctccacg caacgaacaa tttcgttcgg cttttcgcat gcctccttat
     6481 cgccgtgtgg atattggtgg ttcacggctg ctggtaaacg gttcggatga atggatgaac
     6541 aaatcgtggc tggggcatgt gaaaaacatc tggatcaatg tggaagtgtt taatcttctg
     6601 gattttaaga atgtaaattc ttactactgg gtaacagata tttatcagca tcaggtggct
     6661 gttcccaatt atcttaccgg ccggcagctg aaccttcgct tgctagtgga tttcaaataa
     6721 tataattcac gaaatttttt catcagaata taatttattt tttaatggtc tgatgaagcc
     6781 cacatgtctg aatattatat tcattcgttt tgtgtatgca atacatgtgg gtttcttaac
     6841 aacaaatgaa ttttattaca aaatttgtct aaccaaaaat tgtcttcaag aatggttccc
     6901 tgtaagattt cgctaataga aatcaagaat tttttatcgt cccgcttgcc ggatgtaatt
     6961 tctgatttgc cgattaaaat catcttcttt cagtaaatct tccaaattca aatgcaagcg
     7021 ataacgccag ctttttttgg aatcggaagg cacattaatt ctttcttcat ggggattttc
     7081 tcttcgtaat ttttccgaca cgcccagtaa atcctgaatt tgaaaaattg cccagatagc
     7141 gggtgaataa acatgttgga gcagaatgtc ccgacatatc caccattcgc aaaaataagg
     7201 tgcttcgccc caatgcccca attgcgtatt gtaaaaacgc tgcgttatgt ttctgtcttc
     7261 ttcccaccaa cctcgaatag tactcatatc gtgagtggaa ggagtaacaa ccgacaaata
     7321 aggcgcatcg ctcggatgga aaaactctat tttgttggtt ttcggagcac gctgtatttc
     7381 aaggctcaaa attcccaatt ctttcatggc tcgggttacg caaggcgtca tcatgcccaa
     7441 atcttcgccg caaataagca tatcggttgt ttgtttgagc acgggaagtt tttccattcc
     7501 cgattgaaac caaaattcat cttgcctatg gtaaaaataa tcaatgtaaa tttcgtccaa
     7561 cttttgtttg gtaaagtcgt cgagatattg gaaagttgat agcgaatgca ttccccaacg
     7621 cggataaaag cgcgttccat gcgaaccttc cacttcaaaa aacagcacat tagaaatcag
     7681 atcaaacaat ccgtttctta tattttccga aaattgtcct tcttcataca attgctgaac
     7741 gtttttctga ctgcgataaa acggctttaa tcgaagtatc cagccatctt ctatttgtaa
     7801 acaatttact tttacccaac ccgcatcagt tccaaaaagc tgctccagca cctgatcgtt
     7861 aataaatggt ttgcagaagc ggttgtaatc gaaccaaatt ccttttgctt gaaattcatc
     7921 aagataaacc gggatggaag gatacaaatg ccccataata ccctccagct ggtcattggg
     7981 aatttcccaa attcggaaaa acccgagaat gtggtcgata cggaaagcat cgaaatagtt
     8041 ggacatttgt ttcaatcttt ttttccacca gtcaaatcct gtttgtttta tagcttccca
     8101 attgtaagtt ggcaattccc aattttgccc ttttatggag aacatgtcgg ggggtgcgcc
     8161 ggcttgcata tcaatatgga aaagttccgg atgcgcccat gtttcaacac tgtttttatt
     8221 gacgccaatt ggaatatctc ccttgagcac aatgccatgc tgatgagcat aagaggcagc
     8281 ttccattaat tggatatgca aatgatactg aatgaaataa ttcagggcaa tatctgcgta
     8341 atggggctga tttgccgaaa caaatgcggc gattttttca gcattgaaag tggaaaattc
     8401 tttccattga tgataatcgg gtgtaccata ccggtctctc aaaacacaaa aagcagcata
     8461 cggaatcaac cagtgtttgt tgttttcaaa aaaagtcaga aaatctttat tctgcaaaaa
     8521 ctcttcctga tgaaaagaaa aaagagcttt aacatacttc agtttgaaat tgaccacttc
     8581 gagaaaatca atccattcca aagcattcaa ttgcggttgt cgctcctcgt attctttttg
     8641 caacggatga ttttcgggca gttttcccaa cgagggtaag ttaagataaa gcggattcaa
     8701 cgcaaaagcc gagatggcag cgtaaggaag aatatcggca tcggtgtgcg taccaatggt
     8761 atcgttgagc ggaagaacct gaatcaactt taaaccggct ttttcagccc aatccacaag
     8821 caacctgata tccccaaaat cgcctacacc aaaactctct tttgtccgca gactgaaaac
     8881 cggaaccgaa acaccggctc ctttccatga ttgcgaattc attcgcaaaa aaccgtctga
     8941 aagacgaaca aatgtgtttt ttgcattgaa tacgggtgcc gttcggtcaa aaccattttc
     9001 aaacattaaa aactgtccgt tttcggtgtc gtaaattcca tatttataat gaacgtccga
     9061 cttttcctta cccagatcga cttctgcctt ccaccaattc tcaccgtctc tggtcaaaag
     9121 taagggcgaa accgtattcc aactgcccaa cactttgcaa tctccaatca gacaaggaac
     9181 ttgatgattt ttcagcaaag gagccttgat tctgaaaata tgggtaaaat tcttttttga
     9241 acgagttttt tgtgagtgat tgaaattgtc gctgaaaaaa acttttttga aaggagccgt
     9301 aaaaaataca ttttcgaccg ctccgggagc attccaaaaa tcgaaacaga aaatatgctc
     9361 cattcttggg tcaatggtta ttttacgatc ttcaatccat tcgtaagtaa aacgttttgt
     9421 gttttcttcg taaagtaaat atcggtactc caactcaaaa tgttcacttt gatgaatctc
     9481 tatttctcca taccagtctt cggtattttg aaaatgtaac tgaaacgatt tttcaggaac
     9541 attgtttccc aactccggaa gattgccaca aacaaaaatc tgctgtcccc aatgggtgta
     9601 gtaaggtatt ttaaaatgaa ttttcataat ttagaaataa gtgtttcaac tacattaaca
     9661 ttttcaactt caaaaataaa tttatttcta ttaaaatgat gtttgttgac gtaaataatt
     9721 atacaaaaat ttatacaaac gtttgcacca attttaattt ataaaattcc aaaaataaaa
     9781 ttctttgttt ttagtagcaa ttattgggtc atgtgaaaca tgatttgaaa agagaaaaat
     9841 tttttatgaa tcaaaagtct gtttttacta ataaaatgag taattttgcg aaaaatattt
     9901 aactcaaaaa ttaattattc atggcagctt caaactcctc atttaaagtg ttttccggaa
     9961 caaacagtcg ttctttagcc gaaaaaatct gtcaatcttt gggatgcccg ctgggaaaaa
    10021 tgaacattga gcattttgcc gatggcgaat ttgctgtttc ttacgaagaa tcgatacgcg
    10081 gattaaatgt ttatttggta caatccacat ttccgaattc ggataatttg atggagttgc
    10141 ttctgatgat tgatgccgca aaacgagctt cagcctataa agttattgcc gttatccctt
    10201 atttcggatg ggcacgtcag gacaggaaag acaaaccgcg cgtttcgctg ggagcaaaac
    10261 ttattgccga catgttgagt gtggccggta tcgacagact gattactatg gatttgcatg
    10321 ccgaccaaat tcaaggattt ttcaatgtac cggtggatca tttgtatgca tcaacaattt
    10381 ttgctccgta cattcaaact ttgcgtctga aaaatctggt gatagctgcc cccgatgtgg
    10441 gtgcttccaa acgtgcaggt tcgtattcga agtattttga cgttccaatg gtgttgtgcc
    10501 acaaaacgcg cgaaaaagcc aatgtggtgg gagaaatgag aattattgga gacgtaaagg
    10561 gaaaagatgt ggttattgtt gacgatatgg tggacacggc aggaacttta tgcatggctg
    10621 ccaatttaat gatggaacag ggagccaata cggttcgtgc cattgtttct catggcgtga
    10681 tgtcgggaag tgcttccgaa aaagttgaaa aatcggcact gacagaattg gcttttaccg
    10741 attccatccc gtttgatgcc tcaaaatgtt ccaaagttaa aatactttcc attgccgata
    10801 tgtttgccga cactatttgc cgcgttcagg aacataaatc tatcagcgaa caatatattt
    10861 tctgattttt tttggagtaa aatccgaaaa atgaaaaaat tttttatccg gcatttttct
    10921 gttttattaa cgagttaaga cagagaaatg ccggaaatgt tttgacaaaa attgactttt
    10981 cgattcgatt acttgcctaa aattctttcc accaccaatt cagcactgat aactgccatt
    11041 ggcagaccgg cgccgggaat ggtggaagct cccacgtaaa aaagattgtc gtgtttttcg
    11101 tcgtaatttt ttggtcggaa agctccaatt tgggtaaatt tatgagacag tcccagtcca
    11161 ctaccctgat acaaggaaaa tttatctcgc cattcctgag gtgtaaatat cgtttttgaa
    11221 ataatttcgg tagaaatgtc ctttttgatt cgttgtgaaa agtcgtcaat aatgctgttg
    11281 acaattttct cgcggtcgct ccaatcgttt ttgtatttca aattgggaac gggacaaaca
    11341 aaaaataaac tttcacaacc ttcgggagca cattcgggat tatattttga aaccacgtta
    11401 acataatagt atggtttttc gagtgtatcg ggattttgta aaatattgcc ggcgtattgc
    11461 tcgtagttgg accccaaaaa ataattgtga tggtttactt gcggaagttt gcatttcaag
    11521 cccagataaa aagttaaata tcccattgtc cattccattt tatccagttt tttttcagaa
    11581 aaatttttgt atttcataac tttcgatcga aacagtgccg catcggcatt gatcacaaac
    11641 ttgtcggctt tccataaatt gccttgagca tcttttaacg aaacaatttt tcgattttcg
    11701 gcatgatatg caacaatttc tgtctcataa tgaaatttga cattcaattt agaaagttct
    11761 tcgagcattc cttcaacaat tttgtacatg ccgcctttta cgttgtaata gccatcgtgt
    11821 cggaattcta catgcgaaag taaactgtaa attcccattg tatcaaacgg cgtcctcccc
    11881 agaaaaaaag ctaccagcga aataatttcc cgtgcttgct tcgaggtaaa ataatcgcat
    11941 acatgttgcc agaaagtttt gaacaagatg ggcaaatgaa caggattaac tttcatcaac
    12001 gcaagaaaat aatccagata ggaatcgaaa tttcttttta tcacaatatc taccgtgtca
    12061 ttgaacaact gtgcagattt ttggagatat ttttccattt tcagttcaaa atccggttcc
    12121 acatcatcaa actgtttggc aagtttatga atatccttat ataaaaaata agttttgtct
    12181 tcaccactga aatttaccga ataaagggga tcaagttcaa taaactcaaa aggcagattt
    12241 atcccgcatt tctcggcaaa ttccttaaaa atataggaca tgctgaaaaa acttggcccc
    12301 gtatcgaatg taaaaccatc tttcttaatc tgatttaacc tgcctccggc tttggtgtcc
    12361 ttttccaaaa attctacttc gtatccttct tttgccagcc gaagtcctgt tgccaatcct
    12421 ccaagtccgg cgcccacaat gatcactttt tctttcatat attatttttt ttgagtcttt
    12481 tttaaaaatt caggatataa tttgtttttc acccaagcaa aatcgggttc aacatttaag
    12541 attttctgat agtaaatttt tgccgattcg tcattgcccg tgttttcgta agcttgagcc
    12601 agagtaacaa gcaggctcaa ataattccaa tcgtttttaa gtcggtggtt attggacaat
    12661 atcaactttt ctgcctgacg gtaactttca atggcttttg ctttagaacc accaaaagca
    12721 gcaggcgaat aatagtaaat attacccatt tgcagataac caaagtaatt gtttgggtcg
    12781 tattgaatgg ctaatttggc ggcttcaacg cttttgggtc ccaacacagg tgcctttaat
    12841 ttgttcatac ctatttgaaa tccgtaaaaa gccgatttat atgcttcaat cattgataag
    12901 cgaaattgat tttttttcga gagttattag cctgttttct gcttttttta aatattcttt
    12961 ggcttctgca gtttttttgt tcccaataca ccaaccaatg tatccatatt cataatttac
    13021 cagctccaga agaaattcgt cggaagcatt gcctgacgaa aggttcattt tatcaatcgt
    13081 atttttccaa gcctgcatgt cgttgttaat gtaggctttg taaatatttt ccctgtggga
    13141 agcatgaaca tgcagatgca gcaaaatcaa caaaaaaaat aaaactaaac ttttcttcct
    13201 ttccatagat attgattttt cgagcggttg atgagcgcct gataaataaa aattccgaga
    13261 gaaaactgtt ggggaatgaa aaaaatgaga ttatccaata tactttgatt gcttgccagc
    13321 gaaaaaaata tcctgataca gacaacagca agcaagtaaa cggcaagtgt cggtaatccg
    13381 aatgcgagcc atactgctaa ccatccaaaa gtggtaacaa tccagaataa aacagcaatc
    13441 aaaaaagaat ttccaaaaaa atgaattaca tttttagaaa atccgtttac cgcttcagca
    13501 aaattttcat acatccgaca tcttacttgt ttaattccgg tgagacaggc aattttaagt
    13561 tttagcttct tgtaaaacct tgcaatttcg atatcttcca cccgcttgtc tttaaatttt
    13621 tcatgtggtt gaaatttttt ataagtatcg gcatcaaaca gcataaattg tccgtttgct
    13681 gccgaaatag atgggaaaga tgattttctt accaaaggta gcaacagcaa agtcaataaa
    13741 atgtaattca tgtttggaac cgtcattttt tcgccttttg tcatcattaa ttgagtcggg
    13801 aaaatggaaa gcaaatgcag cgatttttgt ttgaaaaatt ttaccgatga aacaacaatt
    13861 tctttttcca tccgaacatc tgcatccaga aacaaaaaat aatttccctt tgcagcgttg
    13921 gcaagcgaat aacaggcaaa atttttcccc agccagcctg ctggcaaatg ggtggaacga
    13981 atcagccgta ttttttcgtt cgtaccaata aattgattca ctatttcaac tgtatgatcg
    14041 gaagaaagat cgtcaaaaac aataatttcg agaatattgt cgtcttcgcc ggacaaatca
    14101 tttaaaagat ttccaatatg ttgttcctca ttacgagcgg gaataagcac cgaaattttt
    14161 tcgtcggaaa tccggtttgt cgaaattggt attttttcac ggaaaaaata attcaacccg
    14221 gcaatgcaca gttgaaaaaa agtaaaacca aaaacaaccc aggctaagta tatcataact
    14281 tattctgttt tagatttttg aatatcgaga cagttgccgt aaaaaacatt ataggcttct
    14341 tgcaacttgg aatgcgaaaa aacatttcct tgatattcgg acacatacat gtacaaatcg
    14401 ggttttttgt gcgaaagata atcgataaaa ttggccacaa ataccagttg aaaattattt
    14461 ttgtgtcctt caacaattct ttcgattcct ttttcgaaaa cgtgaagagg attgtataac
    14521 gactgaagtt ttccttcggg aaatattaaa agcaaattgt cggggttgtc caaaattttg
    14581 gcagcatact gtaacgattc aatcatttcc ctgcttttct tttttatgga aaatccgccc
    14641 gtccactgaa agaaacgaaa cttcaaaagt tggtcttcca acatcataaa ataaaacttt
    14701 ttgtggaata ctttcaaatt cagatacatt gcccaaaacc cgtcccacca gctaatatga
    14761 ttggctatca tcaaaacggg caaattttcg ttccgagaaa aatttgttcg aataacgtta
    14821 accgaatgaa aattcctttt catcaacaaa agtgtatatc gacgaaaaaa aggataaatc
    14881 aggcaatgat gtttggcttt caacatagaa cctttttaga ggatgaacat aaggataaga
    14941 aaaaacaaga attgagccga aaaaacgatt ttactcaaag gattttttaa tttaagtccg
    15001 ctgattacca ataatgtatg aaaaatcaaa gccatgagaa accaaaccaa ataattccga
    15061 aaagggacgc ggtctgcatt ccaaaaccac atttgcattt tgggagcaac ctgctcaagt
    15121 accacatcat acaaaaccat caaaacagaa ggaaaaagta caagaaaaaa tttgttgatg
    15181 cgaaacgatt gaaaaataga agccgtgaga taaaccagca atgcccagtt taaaccaata
    15241 atcagcggag taccaaaaca tttaacgccc aaaccgtttt cataaaaata ctgtccgaat
    15301 attttccccg tatttacgcc aatagcttcc actgtaaaag aaacaacaaa aataacggta
    15361 aatattacgc ccgttttggt actaaaatca tcttcctgaa aaggcaggag gatgaaagcc
    15421 gaaagcagca aagccaacgg gatcaaataa acaaaaaaca catgcgttgc cggcagcacc
    15481 aaaccaacaa ttcctatcgc ataaaaggtg aaaatgaatt gtcgaacttt cctttcggga
    15541 tatttctgct tgaatttttc aagtaaaagg cttatctgca tgaactttct gttaaatttt
    15601 ccgtatcaat gtatgctttg actttcttcc atattatttt gaaccaaacg gtgtactttt
    15661 cgggatgatt ttcaacatcc tccagcagtt gtcggtaggg aatgtatttc cattcttcta
    15721 cctcaccggt gttgtgaatg ggaacgtcat cgctcacacc gaaaaaaaca tggtccaatt
    15781 catgctctgt cagttcatca ttaacttttt cctgataaat aaaatgaaaa agtggaatta
    15841 attcgcaatt tattcccatt tcgtatttta gccttcgttt tgctgcttcg agataacttt
    15901 catcaggata gggatgcgaa caggttgcat tcgtccaaag tccgttggaa tggtatttgt
    15961 ccaaagctcc ccgatgtatc agccaatcgc ctttggagtt cacgataaaa acagaaatgg
    16021 ctcggtgtaa agcagctttt ttgtgggctt catatttttc catgattccc acaggctgat
    16081 cattttcatc caccaactga acaaaattct ctttatttgt ttccataaaa ggctcttaaa
    16141 taatatttta tttgcgttga caaaaccaaa aacattttcc cggcatcaga aattctgatt
    16201 cttttgttaa gaatatcgca ggcagcagtt ttttcaattt ttcttaacag tgccgtataa
    16261 tatttgtagg caatcaaaac ccccagtctg ctgtcagccg gcaacaatac gattcccttg
    16321 taggcatcgt caaaatcttt tcgaatatcc tcaaccagtt cttttttcgt ttgttcggtc
    16381 aacccttctt caacaatttg tggaaaataa tgcctgccca gctcttgcgt atcctgctta
    16441 atatctctaa gaaaattaac tttctgaaaa gctgatccaa gtttggtagc cggcagcatc
    16501 aatcggtcga acaactcttc atctcccttg caaaaaactt tcaaacacat taatccaaca
    16561 acatctgccg acccatacac ataagtattc agttcaattt gacttttgta ttcttttttt
    16621 tccaaatcgg ctttcatact tgagagaaaa tgttgcacat attcatccga aattccatat
    16681 ttacccacag tcatttgaaa cgaatgaagg atgggattca ggctgatacc gtttttcacg
    16741 gcatcataat aatcggattc aaatttccga agcagatatt ccttatcgga aaaatgaaaa
    16801 ctgtcaacga tttcgtcggc caaccgtaca aagccatata tgctgaaaat ggcttcctga
    16861 atttcttttg aaaagtattg cgaagcggaa taaaaggaag tactgtagaa ttttgtaaca
    16921 aacttgctgg tagaaaagct aagggcattg tattttttgt ccataatttt ttggagaaat
    16981 ttgttaatca ggataaaaac ttaattcttg tattttaaaa atttttattt tgtaatcgga
    17041 taccaagtat tgttgattaa tcaaatttaa cttaatttta tcggtattgt ccgacaaaac
    17101 attcattttc aataccattg ttacatactt tgctcatatt aatttgcttt tagtttccag
    17161 aacttcatat ttagtattta acttttgttg gccaaaaagc ttgtattcca ttaaatttca
    17221 atccgtaaaa tttggtcgaa acttatttct cgagcagttg tgattttaaa atttaaacc
//
LOCUS       DAOSCH010000040        17043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2321_length_17043_cov_6.730633, whole genome shotgun sequence.
ACCESSION   DAOSCH010000040 DAOSCH010000000
VERSION     DAOSCH010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 17043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17043
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2321_length_17043_cov_6.730633"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..906
                     /locus_tag="PLZ54_04610"
     CDS             331..906
                     /locus_tag="PLZ54_04610"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOJ66245.1"
                     /translation="MKFQLPELPYAQNALEPVISERTISFHYGKHHLTYVNNTNNLVV
                     GTEFENQDLETIVKKATGPLFNNAAQIWNHNFYFLSLTPDKGTKPSEKLLKAIEAEWK
                     SLDNFKIEFGKAATGLFGSGWAWLVKDASGKLSIVNESNAGNPMTKNLSPILTFDVWE
                     HAYYLDYQNRRADYVAALWDLVNWSIVSERF"
     gene            1094..1339
                     /locus_tag="PLZ54_04615"
     CDS             1094..1339
                     /locus_tag="PLZ54_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66246.1"
                     /translation="MFYTLLITLLIVLLSVLLLGFRIFFIKSGKFPNIHIGGSKALQQ
                     RGIGCATTQDKEAQRNFHKGIDVSNLLTEISEQLKNE"
     gene            1423..2031
                     /locus_tag="PLZ54_04620"
     CDS             1423..2031
                     /locus_tag="PLZ54_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOJ66247.1"
                     /translation="MKNLLFVSNIVLFIAVVVLFVLFFQIKPAKNAVQLSLKGDTLSS
                     QVLPIAYINVDSLLLKYRFAKDANESLIKKQEDSRLTINSRARELQNEMNEFQRKLEN
                     NAFLSRERAEQEHTRLLKKQQELQNLDGQLTQQLLQEQQKMSEELRDTINAFLKEYNK
                     DGRYEIIFSNTTGDNILFASKSYDITDEVVKLLNERFSNKKK"
     gene            2267..3913
                     /locus_tag="PLZ54_04625"
     CDS             2267..3913
                     /locus_tag="PLZ54_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005939031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOJ66248.1"
                     /translation="MLKPEYIFETSWEVCNMVGGIYTVLSTRAATLKEVYGDKLIFIG
                     PDIWIQKNNPLFVEDSRLFPGWKDAVQQQFQLNIRIGRWQIHGNPIAVLVDFMPLMLR
                     KDEIYGHVWLQTGVNSLPAYGDYHESSMFGYAAGMVIESFYRFFGLSLKNDVIAHFHE
                     WQTTFGIFYLKEHLPEAATIFTTHATTVGRSIASNHKPLYDYLQEYNGDQMAAELNVV
                     SKHSAEKTAAHLSDCFTTVSDITAAECRQLLEIKPAVVTPNGFEDDFVPKADTFIQKR
                     QEARTCLRKVAETLLGYSLQENALFVGTAGRYEYKNKGIDVFIESMRRLIECPHLNRE
                     IVVFIMVPAHVMGPRTDLLAALNHPNLKLHSWNLFTTHELYDYQNDHIMSAIRWFHFT
                     NQSSQKVKMIFVPSYLNGSDGIFNKKYYDLLIGLDVTIFPSYYEPWGYTPLESIAFSV
                     PTITSDLSGFGRWVAKQPQDITEGVGVVHRSDYNQYDATVKIAQMICQFSSLEQSQVE
                     NIRSIAASISLKALWENFIQYYYEAYNIALTQKNLRLGKH"
     gene            4146..6710
                     /gene="glgP"
                     /locus_tag="PLZ54_04630"
     CDS             4146..6710
                     /gene="glgP"
                     /locus_tag="PLZ54_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOJ66249.1"
                     /translation="MSLKLNQVNEPRWKEINVKSFLPDGLKKLEEIAQNIFWVWRSDA
                     RHLFRELDKDAWAESGYNPVQLLNMLSYERLVEVEKDAHFMSNLDRIYNEFQAYLHQP
                     FAPDKPSIAYFSMEYGLTEVLKIYSGGLGILAGDYLKEASDCGVDLTAVGLLYRYGYF
                     TQSLSVDGDQIEIYDKQNFNTLPIVQVRDENGQAVVIEVPYPGRTVYANLWKVSVGRI
                     NLFLLDTDNEMNSEADRSITFELYGGDWENRLKQEIMLGIGGILTLKKLGIEKDVYHC
                     NEGHAALINLQRLADLVTEKKLSFHEALEIVRSSSLYTVHTPVPAGHDRFDEALFGKY
                     MKDYAALLNISWNDLMNLGRENPDDPNDKFSMSVFACNTCQEVNGVSWLHGKVSQEMF
                     QPIWKGYYPEELHVSYVTNGVHLPTWTTSEWKGVYEKYLGKDFYDDQSNMKLWQAIYE
                     VPDQVIWETRVKMKNKLIDYIRLQFQENWLKSQGDPSRIMSVLESMNPNALIIGFGRR
                     FATYKRAYLLFTDLKRLSKIVNDPAKPVQFIFSGKAHPADQGGKALIKRIVEVSRMPE
                     FLGKIIFLENYDMHLAKRLISGVDIWLNTPTRPLEASGTSGQKAQMNGVLNFSVLDGW
                     WLEGYVKGAGWAITEKRTYDNDAYQDQLDAATIYSMLENEIIPLYFAKNSKGISPEWI
                     QYIKDSIAQITPRFTTKRMIDNYVEQFYTKLSNRHALLKDNNFARAKEIAAWKQKIAK
                     GWDKIEVVSMDIPEGLLKNPQVSEVYHMNIVIDIKDINDKGIGIELVITKPDKDNKFY
                     LHDVEEFKLVKANGSLLYFSLEYQLNRAGLFQFGFRMFPKNEDLPHRQDFCYVKWI"
     gene            complement(7177..8340)
                     /locus_tag="PLZ54_04635"
     CDS             complement(7177..8340)
                     /locus_tag="PLZ54_04635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ66250.1"
                     /translation="MYEEILCITHKYPPFTGGMEKQSYELIQGLSSYYRVHIIAFEGK
                     ENKLLWFLKLKSRVKKLLKANPNIKLIHLNDGAMGVAALWLQKITQIPVVVTYHGLDI
                     TYPLTFFQKKLIPKLIKYKGAVCVSKFTSEQCIKRGFDPRTTFIVNNGVDLKLNEIPF
                     DSAIAQKLGEKYGIDVSGKNIIMVTGRPVKRKGFSWFLKNVMPLLYNDVILILTGPLK
                     KSQTFFDIFLRCLPKMLRHKIQLLFGFPDDNKEVIEQLKKQKNAFHLGRVSNEDLMQI
                     ISLADLFVMPNINIPGDVEGFGLVALEASVRGTYVLASKVQGITDAVIDMENGTLLPC
                     ENAEAWADKIKEMLSDKEKLKILSEKGRNFTIQHFSWQKMVDEYKQVFDGFIH"
     gene            complement(8340..9347)
                     /locus_tag="PLZ54_04640"
     CDS             complement(8340..9347)
                     /locus_tag="PLZ54_04640"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOJ66251.1"
                     /translation="MSKLKRYRKLIISVLMISVSVLIIRFISSIDFHILNMYLQRMPS
                     MFLLVIAISFVGYFFSSLGWKLCMGEEGMNISIWKIFIIRHIGEMLGAFNPTSIIAGD
                     SLKISYLVKSGISTKIGLSSILLMRMITVLSSIFLIVVSIFYLIFSKFGNGKTFVLFL
                     LIALVMISGFLMAKYLLDDHLYLGKTLERIKSKTNWSFLTSKTIDSCYEINTLASNYF
                     KHHKLNFLLAFLLLTLHWVCGALEFFIILQTLHVNISVIDAISMEVGVSLFKSLGAIV
                     PGQIGIEEYGNKIMLDVVAVKGNEIWFVVSLVRRSRQLFWLSVAGVFFLITKRKKFTL
                     Q"
     gene            complement(9394..10620)
                     /locus_tag="PLZ54_04645"
     CDS             complement(9394..10620)
                     /locus_tag="PLZ54_04645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOJ66252.1"
                     /translation="MYSNKKIKVAFFADILTRDHDGAIKTMYQLIDKVPEENFEFMFC
                     TGTPPKEKINHKILQIPSLTLPFNSNYKACLPHFSSNKIVKALEDFDPDVIHIATPSP
                     LGFFGLNFARKKHIPVLSIYHTNFLSYIQYYFNRLPFLIKPVESIMKKIYQSFYNFCN
                     MVYVPTKQMIIELKDSGVSHQPLKLWQRGIDSNLFNPSKKDMHYIQQITGNNKPCILF
                     ASRLVWEKNLETLFNIYDETEAQNLDVNFIIAGSGVAEQAARKRMKKAFFLGFLNHEE
                     LSKVYASSDVFVFPSISETFGNVVVEAMASGCVPVIARGGGSQSLVMNGVTGFLCNPY
                     DAREYVENIKVILNYPSLRESIQQNAIRFASNFNWNRLANEYFSDLQMLAYKSSPTFE
                     DNVVLTNELFPTNYAI"
     gene            10976..12601
                     /locus_tag="PLZ54_04650"
     CDS             10976..12601
                     /locus_tag="PLZ54_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3843 family protein"
                     /protein_id="HOJ66253.1"
                     /translation="MSTKSTDRINLKQWLELKPYSQQTSTDKYYVELANKVKNVLTKK
                     QPSAFLNEYLNEDEIDILCCFLVSYLEDIVTGSNIWNTFTKKHFSLYKKYLPFFPLEN
                     YILNNVNMQDVNFLIWYFLNTIQQNFFISPINEEIMDMAFEVKNILSDEYKYAPENPI
                     LKSYYQLNRDETNYFSVRGLIELILFNTYLFYPDTYIRFKKENEKEQKNKQKSVDKDR
                     EAMLFYEIKDNFINTSNTSLLAMTGKEWAAELLGEKHPLYKDVINISPKVKGFFLYKG
                     QDKNNIFIEHIASGRKFEMTKKSYEHYNDLKKVDTILLIGIVKWRGEWWFSGTSSKMD
                     YNKKLIEDVKHTLAFKQTVNFLDFEEFEGNKLIKNQMMAFLNANNGNFIAFMKRSQVN
                     EFIEKVTNCYNDKLNISEDEKERFKKNAKQKKDLKLEKEDEISADELDDPCIVFFNPH
                     SGIEIAVGINSAFPMKSNPFFHPEKSETDFWQLLLSNFYSAELVQFCLDTCKNNLPFL
                     RKKVWKTYLKDIDFVLRFLKVDSYHSKPEITFL"
     gene            13159..13935
                     /locus_tag="PLZ54_04655"
     CDS             13159..13935
                     /locus_tag="PLZ54_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOJ66254.1"
                     /translation="MNGIVNLSLVPLRAEDNECSEMISQLLFGETVEIMEWKERWLHI
                     RNLSDNCCGWADRKMITLLSEDEYENLKNNENFRILKAVSFCRNLTLGTTFLLPGGSI
                     LRKFQNGNFTDGTYQYAIHPSDAGMPKPLTGGNIVSLARQYHFTPYLWGGKSALGIDC
                     SGLVQVVFSLCGVLLPRNASQQAEIGEKINSLTQAEAGDLAFFANSAHHIVHVGILMN
                     SHQIIHASGWVKIENIDEHGIISEQTGNYSHTLYSIKRMI"
     gene            14084..14593
                     /locus_tag="PLZ54_04660"
     CDS             14084..14593
                     /locus_tag="PLZ54_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ66255.1"
                     /translation="MDAAQFNSVILPLADQLFRVAKSIVCNDEAAKDAVQELNAKLWE
                     NRSQLDKVQNLTAFAMKSMRNLCLDWLRTRKSDYDVPLDENLIASAPNPHQLMEQKDL
                     TNYVKQLIDKLPELQRTIIHLRDVEGLEISEIAAITSISENAISVNLSRARQKLRTEL
                     MEKSKKEKI"
     gene            14643..15128
                     /locus_tag="PLZ54_04665"
     CDS             14643..15128
                     /locus_tag="PLZ54_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66256.1"
                     /translation="MKEIDDLLNKYFEGKTTLEEENYLKSYFRNEEIQPEHEVYKTLF
                     HVFDEKAKEKIPQPVDEKNAQPNVKRFWKLTAVIAGIAAAFLMIIWVMPIEKNQDFAI
                     VNGEKIENTQFAQNYALEKLNHVSYKLETNLKPLEAIATVKSRMEPVKKIPEIKKEWK
                     K"
     gene            15166..15654
                     /locus_tag="PLZ54_04670"
     CDS             15166..15654
                     /locus_tag="PLZ54_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HOJ66257.1"
                     /translation="MKKNGILILTILLLASFRAGAQTIDKFFDRYANDDRFTYVSVGK
                     GMMNLASTFGANKNMDKNQKEVLDRLTGIKILTLENSRDEKLMNSVLDELDKVIRAGK
                     YETAVRVKDKDETVNIFFRTTGKDQSDMIIVSKEKNEMSIIWMNGKMTKEEMSDIFSQ
                     NS"
     gene            complement(15922..16266)
                     /locus_tag="PLZ54_04675"
     CDS             complement(15922..16266)
                     /locus_tag="PLZ54_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ66258.1"
                     /translation="MCAKILFREYPDLKQGYALTHSLRMIYNKNSIKAAARLSLAKWY
                     NNVAESQFKSFNTIAATVYEHHEEVLNFFINRSTNASAESFNAKIKSFRASLRGVIDI
                     KFFLFRLTNIYA"
     gene            16575..>17043
                     /locus_tag="PLZ54_04680"
     CDS             16575..>17043
                     /locus_tag="PLZ54_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008999622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOJ66259.1"
                     /translation="MNEVIVTGTRSEADVRNLPMSLSVITDKQILDRQSQSVIPLLNE
                     LVPGLFITGRGIMGYGISTGAAGTMKMRGIGGNPTTGVLILIDGEPQFMGLMGHPIAD
                     LYQSVIADRIEVVRGPASALYGSNAMGGVINIITSKFYKDTVQQNFHFGYGSYS"
BASE COUNT         5693 a         2895 c         3101 g         5354 t
ORIGIN      
        1 ccgggggttc gaatccctct ctctccgctg aaaagtcctg tatttcaggg ctttctttgt
       61 atataaacat attgaatcgt ttccctttat cgacttcaaa ttccattggt caaaaaaatt
      121 ctttacttaa aaaaattggt atagttatta atttgaaaga taattatttg gatttttttt
      181 tttaacagaa ctatagaaat tttgaaaagt aagagataat atccgttgat ttttttttgg
      241 gtaaatatta aaacaaaaca gaaaattgat tgttataaca ataaattttc tataaaatca
      301 atttagtatt aaccataaaa ttttattgtt atgaaatttc aattgcctga attaccttat
      361 gcacagaatg ctttggaacc ggttatcagt gaaagaacaa tcagttttca ctacggcaag
      421 catcatctaa cttacgtaaa caacaccaac aatttggtag taggtacaga atttgaaaat
      481 caggatttgg aaaccattgt taaaaaagcg acaggtccgc ttttcaacaa tgccgcacaa
      541 atatggaatc acaatttcta tttcctttcc ttgacccctg ataaaggtac taagcccagc
      601 gaaaaactac tgaaagccat cgaagccgaa tggaaatcgt tggataattt caaaattgaa
      661 tttggcaaag ctgcgaccgg attgtttggt tcaggctggg cttggctcgt aaaggatgct
      721 tcgggaaagc tttccattgt caacgaaagt aatgcgggga atccgatgac aaaaaatctg
      781 tctcccattc ttactttcga tgtttgggaa catgcttatt atctcgatta ccaaaaccgg
      841 agagccgatt atgtagctgc tttatgggat ctggtaaatt ggagcatagt gtcggaacgt
      901 ttctgattat tttgtttttt acctttttgt ttttttaccg gcttataaca taggccggtt
      961 tttaatttat tgcataaata tttaagaatt actaataaag caaataaatt atttgttttg
     1021 ctaaaaaatc atagaaataa tttcattttc tattaaatat cttatctttg caagccgtta
     1081 tatttttaag tttatgtttt acaccttact tataacctta ctgattgttt tactttcggt
     1141 tttattattg ggatttcgta tcttcttcat taaaagtgga aaatttccca atattcatat
     1201 tggtggaagc aaagccctcc aacaaagagg tattggctgt gctaccacac aggacaaaga
     1261 agcccaacgg aattttcata aagggattga tgtttccaat ttgttaactg aaatttctga
     1321 acaattaaaa aacgaatagg ttgtttcatg cagattgtct ttttgaattt tatttttgtc
     1381 tttgcatggt atgaattaaa gataatattt taaagcttaa atatgaaaaa tttgttattt
     1441 gtttccaaca tcgttctttt cattgctgtt gttgtacttt ttgttttgtt ttttcagatc
     1501 aaacccgcta agaatgcggt tcagttgtca ttaaaaggag ataccctgtc aagtcaggtt
     1561 ttacctattg cttacataaa tgtagattcc ttgttattaa aataccggtt tgcaaaggat
     1621 gccaatgaat ccctcattaa aaaacaggaa gattcacggt tgaccataaa ctcacgtgct
     1681 cgtgaactgc aaaatgaaat gaacgaattt cagagaaaac tcgaaaacaa tgcatttctc
     1741 agccgggagc gggcagagca ggaacatacg cgtttattaa aaaaacaaca agaattacaa
     1801 aatcttgatg ggcaacttac tcaacagctt ttacaggaac aacaaaaaat gagtgaagaa
     1861 ttgcgcgata ccattaatgc ctttctcaaa gaatataata aggatggccg ttatgaaatt
     1921 attttcagta ataccacagg cgacaatatc ctatttgctt ccaaaagtta cgatattacc
     1981 gatgaagtgg tgaagctgtt gaacgaacga ttttcaaaca agaaaaaata atttctaatt
     2041 tggctgttca aaaagtatat cctacgtaag taggattttt tttgacaagc aaggtatagg
     2101 atattcataa aactaatatt tagttttttc aaaaatactt ttttatcact tttgtttgat
     2161 tttaaaatca ttgataagat aattaatttt tatcagtttt attttcaatt tttccccgtt
     2221 ttcaaaaaac aacagaaagt ttaatttcga ttataccaaa ttaagtatgt tgaagccgga
     2281 atacatattt gaaacaagct gggaagtatg caatatggtt ggtggaatat ataccgtttt
     2341 atctactcgt gctgcaacat tgaaagaagt ttatggagac aaactgattt ttattggacc
     2401 tgatatatgg atacaaaaaa acaacccgct ttttgttgaa gacagtaggc ttttccccgg
     2461 ttggaaagat gctgttcagc aacagtttca gttgaatatt cgtatagggc gttggcaaat
     2521 tcatggaaat ccgattgcag tattggtcga tttcatgccg ctgatgctac gaaaagatga
     2581 aatttatggc catgtttggc tgcaaaccgg agtcaattcc ttgcctgcct atggcgatta
     2641 tcacgaatct tcgatgtttg gctatgctgc aggtatggtg atagaaagtt tttatcgatt
     2701 ttttggtttg tctttgaaaa atgatgtgat tgcccatttt catgagtggc aaacaacttt
     2761 cggtattttt tacttaaaag aacatttgcc tgaagctgca accattttta ctactcatgc
     2821 cactactgtg ggacgttcca tagccagcaa tcacaaaccg ctgtatgatt atctgcaaga
     2881 gtacaatggg gaccaaatgg ctgccgaatt gaatgttgtt tccaaacatt cggccgaaaa
     2941 aactgccgct catctttccg attgcttcac aactgtaagc gacattactg ccgctgaatg
     3001 ccgacaattg ctggaaatta aacccgctgt tgttacacca aacggttttg aagatgattt
     3061 tgtgcccaaa gccgatactt ttattcaaaa gcgacaggaa gcacgaactt gtttacgcaa
     3121 agtcgctgaa actttgttgg gttattcttt acaggaaaat gctctttttg tgggtactgc
     3181 cggacgttac gaatacaaga acaaaggtat tgatgtgttt atcgaatcga tgcggaggtt
     3241 gattgaatgc cctcatttga acagagaaat tgtggttttc attatggtgc ccgcccatgt
     3301 gatgggcccc agaacggatt tacttgcagc tttaaatcat ccaaatctca agttgcattc
     3361 atggaatttg tttaccacgc acgaacttta cgattatcag aatgatcata tcatgtcggc
     3421 tattcggtgg ttccatttta ccaatcaatc gtcgcaaaag gttaagatga tttttgtgcc
     3481 ctcttatttg aatggttccg acggcatatt caataaaaaa tattacgacc tgctcattgg
     3541 gctcgatgtt accatatttc cttcctatta tgaaccatgg ggctatacac ctctcgaaag
     3601 cattgctttt tccgtaccaa ctattaccag cgatttgtcg ggttttggcc gctgggtggc
     3661 aaaacaacct caggatatta ccgaaggtgt gggagtagta catcgttccg actacaatca
     3721 gtatgatgca actgtgaaaa ttgcacaaat gatttgtcag ttttcgagtt tggagcaatc
     3781 tcaagtagaa aatatacgct caatagcggc ttcaatttcc ctgaaagcct tatgggaaaa
     3841 cttcatacaa tattattatg aagcatacaa tatagccctt actcaaaaaa atctccgact
     3901 tggcaaacat tagaattaag aaaaaaatcg attttttcgg tttttctttg taacgaatta
     3961 ataattaatt aattgaaaaa gaatatatta acaaaaattt tttaataaat tgcaatcgtt
     4021 tgcatttgtt tttactcctt ttatgatttg ttttatcttg gctttttgaa actttgctta
     4081 actttgccta cagagtttta ggtataaaat tttttttgaa tttcactaac ttaaagtaag
     4141 gaaacatgtc tttgaaatta aatcaggtaa atgaaccgcg ctggaaagaa ataaatgtca
     4201 agtctttttt gcccgatggc ttgaaaaaat tggaagaaat agcgcaaaat atattttggg
     4261 tatggagaag tgatgcccga cacctttttc gtgaattgga caaagatgct tgggcggaat
     4321 cgggctataa tcctgtgcaa ttgctgaaca tgctcagtta tgagcgactt gtagaagtgg
     4381 agaaggatgc ccatttcatg tccaacttag acaggattta caacgaattt caggcttatc
     4441 tgcatcaacc ttttgcaccc gataaacctt cgattgccta cttcagtatg gaatacgggc
     4501 tgacggaagt tttaaaaatc tattccggag ggcttggaat tttggcaggc gattatttga
     4561 aggaagccag tgattgtggc gttgatttga cggctgtcgg attactttat cgttacggat
     4621 attttactca gtcgctttcg gtcgacggtg atcaaattga gatttatgac aaacaaaact
     4681 tcaatacttt gcctattgtg caggttcgtg atgaaaatgg acaagcggtt gttatagaag
     4741 ttccctaccc gggacgcacc gtgtatgcca atttgtggaa agtaagcgtt ggacgtatta
     4801 atcttttcct tttggatacc gacaatgaaa tgaacagtga agctgacaga agtattactt
     4861 tcgagttgta tggcggcgat tgggaaaatc gtctgaaaca ggaaatcatg cttggaattg
     4921 gtggaattct gacactgaaa aaactaggca tcgaaaagga tgtctatcat tgcaatgaag
     4981 gccatgcagc ccttatcaat cttcagcgtc tggcggattt agtaaccgag aaaaaacttt
     5041 ctttccacga ggctttggaa attgtgcgga gcagttccct ttatactgta catacacctg
     5101 ttcctgcggg acacgaccgc tttgatgaag ccttgtttgg aaaatacatg aaagattatg
     5161 ctgccttgct gaatatttcg tggaacgact tgatgaactt gggacgtgaa aatccagatg
     5221 atcccaatga caaattcagt atgagtgttt ttgcctgcaa tacctgtcag gaagttaatg
     5281 gtgtaagttg gttgcatggc aaggtttcac aagaaatgtt tcagccaata tggaagggtt
     5341 attatcctga agagctgcat gtgagctatg ttaccaatgg tgttcattta cccacatgga
     5401 ccacttcaga gtggaaagga gtttacgaaa aatatctcgg aaaagatttt tatgacgatc
     5461 agtcgaacat gaaactctgg caggctattt acgaagtgcc cgatcaagtt atctgggaga
     5521 cgcgcgtcaa aatgaaaaac aaattgatcg attatattcg tttgcaattt caggagaact
     5581 ggctgaagtc tcagggcgac ccttcccgta tcatgtcggt tctggaatcg atgaatccca
     5641 atgctttgat catcggtttt ggtcgaagat ttgcaactta caagcgcgct taccttttat
     5701 ttaccgattt gaagcgttta tcgaaaattg taaatgatcc ggcaaaacct gtacagttta
     5761 ttttttccgg taaggcacat cctgccgatc agggaggaaa agcgcttatt aaacgcatag
     5821 tggaggtttc tagaatgccc gaatttttag gcaaaatcat tttcctcgaa aattatgata
     5881 tgcatttggc caaacgattg atttccggcg tcgatatttg gttgaatacc cctacgcgtc
     5941 ctttggaagc ttcgggaact tctggacaaa aggcacaaat gaatggcgta ttgaattttt
     6001 cggttctcga tggatggtgg ttggaaggtt acgtgaaagg agccggttgg gctattaccg
     6061 aaaaacgaac ttacgataac gatgcgtatc aggatcagct tgatgctgca accatctaca
     6121 gcatgctcga aaatgaaatt attccattgt attttgccaa aaattccaaa ggaatttcgc
     6181 ccgaatggat tcaatacatc aaagattcca ttgcacaaat tacaccacgt tttacaacca
     6241 agcgcatgat tgacaattat gtagagcagt tttacaccaa actaagcaat cgtcatgcat
     6301 tattaaaaga taataatttt gcgcgtgcca aagaaattgc tgcatggaaa cagaaaattg
     6361 ccaaagggtg ggacaaaatt gaagttgtga gcatggatat tcctgaaggt ttgttgaaaa
     6421 atcctcaagt tagtgaggtt tatcacatga atattgtcat tgatatcaaa gacattaatg
     6481 acaagggtat cggaatagaa ctggtcatta ccaaacccga taaggacaat aaattctatc
     6541 ttcatgatgt ggaagagttt aagctggtga aagcgaatgg ttcactattg tatttcagtc
     6601 tcgaatatca gctcaataga gcaggattgt tccaattcgg attccggatg ttccccaaaa
     6661 atgaagattt gcctcatcgt caggatttct gttatgtaaa atggatataa ccgttaccta
     6721 ttcggatttt aatcaaaaga tttcctcgag gcttgcctct gggttatttt tttgatgaaa
     6781 gtgcattatg ttatgttgtt gttcacagta tgctcatatt gtaaaaaaac gcagaaagtt
     6841 ttattacgcg agtccactcc aaaaagtcaa atttgacaac tttgatattt gtaaaacaca
     6901 gaatatcaac gctaagcctt aataagaaat taaaaaaaca gacttttaaa agtggactct
     6961 tacactgaaa atttaaactt ttgttactgt atatttagaa aaaatcaaca acaaaccgtt
     7021 attaaatttt gtttcaaata caagaagaca aacgtgattt tcctcactta taaacagttc
     7081 ttggagtaag actttttcat taaattggtt aatgagtaat tttggtttat tacagtaagc
     7141 caaaaaagtt gcaacttgga taaaatacaa tatcttttaa tggataaagc catcgaatac
     7201 ttgtttgtac tcatcgacca ttttttgcca agaaaaatgt tgaattgtaa aatttctgcc
     7261 tttctccgac aatattttaa gtttttcctt gtcgctcagc atttctttta ttttatctgc
     7321 ccaagcttca gcattttcgc aaggaagtag cgtcccattt tccatgtcta taactgcatc
     7381 tgtaattcct tgtactttgg atgccaacac atacgttccc cgaacgctgg cttcgagtgc
     7441 aaccaagcca aatccttcta catcgcctgg aatattaatg ttaggcatta caaataaatc
     7501 ggcaagcgaa attatctgca taagatcttc atttgaaact cttcccagat gaaaagcatt
     7561 tttttgtttt ttcaattgtt ctataacttc tttattatca tcaggaaaac caaataacaa
     7621 ctgaatttta tgtcgtagca tttttggaag gcaccgtaag aaaatgtcaa aaaatgtttg
     7681 ggatttttta agtggaccgg ttaaaatcaa gatcacatca ttatacaata aaggcatgac
     7741 atttttcaaa aaccatgaaa aaccttttcg tttcaccggc ctgccggtta ccatgataat
     7801 atttttaccg gatacatcta tgccgtattt ttccccaagt ttctgggcaa tggctgagtc
     7861 aaaagggatt tcattcaact tcaaatctac cccattgttt acgataaagg tggttctcgg
     7921 gtcaaaaccc cttttgatac actgttcgct ggtaaattta cttacgcaaa cagcaccttt
     7981 gtattttatt agttttggga taagtttttt ttgaaaaaaa gtcaaaggat aggttatgtc
     8041 cagaccatga taagttacaa cgacaggaat ttgagtgatt ttctgtagcc aaagtgctgc
     8101 aactcccata gctccatcat tcaaatgaat cagttttatg tttggatttg ctttcaataa
     8161 tttttttact ctcgacttca atttcaaaaa ccaaagcaat ttattttcct ttccttcaaa
     8221 agcaataatg tgtactctgt aataggagga taaaccctga atcagttcat aactctgttt
     8281 ttccatacca ccggtaaaag gtggatactt atgggtaatg cataatatct cctcgtacat
     8341 tattgaagtg taaatttttt cctttttgtg atcaagaaaa atactcctgc aacagataac
     8401 cagaaaagtt gacgcgatct tctcaccaga gaaaccacaa accaaatttc atttcctttt
     8461 acagccacta catcaagcat gattttgttc ccatattctt cgattccaat ctggccggga
     8521 acaattgcac ccaaagattt aaaaagcgaa acaccaactt ccattgagat ggcatcgatt
     8581 accgaaatat tcacatgtaa ggtttgcagt atgataaaaa attcaagggc tccacatacc
     8641 caatgaagtg tcaataataa aaaagccaat aagaagttca gtttgtggtg cttgaaataa
     8701 tttgaagcaa gtgtatttat ttcataacac gaatcgattg ttttgctcgt caagaacgac
     8761 cagttggttt ttgatttaat gcgctccaat gttttaccaa gatataaatg atcatcgagt
     8821 aaatattttg ccattaaaaa gcccgaaatc attaccaacg caattaacaa aaaaagtaca
     8881 aaagttttcc cattgccaaa tttactgaaa atcaaataaa atatagaaac gacgatcaga
     8941 aaaatggatg acaacacagt aatcattctc atcaacaaga tggaactcaa accaattttt
     9001 gtagaaattc cggactttac gagataagaa atttttaagc tgtcgcctgc aataatactg
     9061 gtaggattaa aagcccccaa catttctcct atatgcctga taataaatat cttccaaata
     9121 gaaatattca ttccttcctc acccatgcac aatttccaac ccaatgaaga aaaaaaatac
     9181 ccaacaaatg aaatagcaat aaccaataaa aacatggagg gcattcgttg gagatacata
     9241 ttcagaatat ggaagtcaat gctcgatata aatcgaatta tcagcacaga taccgaaatc
     9301 atcaaaactg agataatcag ttttcgatat cttttaagtt tgctcattct gttagcttag
     9361 tgaataaatg tctgaggtct aagagttttt taatcagata gcataattgg tagggaaaag
     9421 ttcatttgtt aaaactacat tgtcttcaaa agtaggcgat gacttataag ccaacatttg
     9481 aagatcggag aaatattcat ttgccaagcg attccaatta aaattggatg caaaacgaat
     9541 agcattttgc tgtattgatt ccctcaagga aggataattt agaataactt taatattctc
     9601 tacatattcg cgagcatcat aaggattaca caaaaatccg gttactccat tcataaccag
     9661 agattgcgaa ccgcctccac gagcaattac cggtacgcaa ccggaagcca ttgcttctac
     9721 aaccacatta ccaaatgttt ccgaaataga agggaaaaca aacacatcgg aagatgcata
     9781 aactttcgac agttcttcgt gatttaaaaa cccaagaaaa aaagcttttt tcattctttt
     9841 tcgtgctgcc tgctcagcaa caccacttcc agctataata aaattaacgt ctaaattttg
     9901 agcctcagtt tcatcataaa tattaaaaag tgtctctaaa tttttttccc aaactaaacg
     9961 agatgcaaac aaaatacatg gtttattgtt gcctgtaatt tgttgaatgt aatgcatgtc
    10021 ttttttggaa ggattaaaca aattagaatc tattcctctt tgccaaagct tcaaaggttg
    10081 atgcgaaaca ccactgtcct tcagttctat aatcatttgc tttgtaggaa cataaaccat
    10141 attacaaaaa ttgtaaaagc tttgataaat ttttttcata atcgattcta ccggctttat
    10201 taaaaatggc aaacgattga aataatactg tatgtaggaa aggaaattgg tatgataaat
    10261 tgacaagaca ggaatatgtt tttttcgggc aaaatttagt ccaaagaaac caagtggcga
    10321 tggagtagcg atatgaatca catccggatc aaaatcttca agtgctttaa caatcttatt
    10381 gctgctaaaa tgagggagac atgctttgta atttgaatta aaaggtagtg ttaatgaagg
    10441 gatttgtagt attttatggt ttattttttc ttttggagga gtgccggtgc aaaacatgaa
    10501 ctcaaaattt tcttcaggta ccttatctat tagttgatac attgttttta tggcaccatc
    10561 atgatcccga gtaagaatat cggcaaaaaa ggctacttta atttttttat tggaatacat
    10621 gtttttttat aggttttatt attgttcaga tattgctaat ggctaaaggg ataaagaaac
    10681 cgttcatctt ttttgaaaat agatttttaa aacttctctt ttcaaaactc ttttccacag
    10741 cacaaaagta gaaatggtgc atgacaattt tgtatcacta caattaaatt cctgcttcaa
    10801 aaattctatc atcgaaaacc attttaggat agaggttttt gttgaaacaa tttaacattt
    10861 gaaagtcagt gaattttttt acatttgtat aaaaaaatcg tgcaagtctt cataacgcaa
    10921 tcagtagaaa attctatcaa aattttgatt ataaaataaa aagttaggtc agcaaatgag
    10981 tacaaaatca accgacagaa tcaatctcaa acagtggctg gaactcaagc catacagcca
    11041 acaaacatca acggataaat attatgttga gctggctaac aaggtaaaaa atgtgttgac
    11101 aaaaaaacaa ccttcagctt tcctaaacga atatttaaat gaagatgaaa tagatattct
    11161 ttgctgtttt ttggtttctt atttggaaga tattgttact ggaagcaata tctggaatac
    11221 ctttacaaaa aaacatttta gtttgtataa aaaatatctg cctttttttc cgttggaaaa
    11281 ttacattctg aataatgtga atatgcagga tgttaatttt ctgatttggt actttttgaa
    11341 caccattcaa caaaactttt ttatcagccc gatcaatgaa gaaattatgg acatggcttt
    11401 tgaagtaaaa aatatcttat ctgatgaata taaatatgca ccagaaaatc caatattaaa
    11461 atcatattac caactaaaca gggatgaaac aaattatttt tccgttcgag gtcttatcga
    11521 attgatattg tttaatactt atttgtttta tcctgatact tatatcaggt ttaaaaaaga
    11581 aaatgaaaaa gagcaaaaga acaagcaaaa atctgttgac aaggatagag aggcaatgtt
    11641 attttatgaa ataaaggata attttataaa cacttctaat accagcctgt tggcaatgac
    11701 cggaaaagag tgggcagccg aattgttggg ggaaaaacat ccattatata aagatgttat
    11761 aaatatttct ccaaaagtta aaggtttttt tctttataaa ggtcaggaca agaacaatat
    11821 tttcattgaa catattgctt caggaagaaa attcgaaatg acaaaaaaat cttatgaaca
    11881 ttacaacgat ttgaaaaaag tagatacaat tttgcttatt ggaattgtga agtggcgtgg
    11941 agaatggtgg ttttcaggta cttcttccaa gatggactac aataagaaat tgattgaaga
    12001 tgtaaaacat actcttgcat tcaagcaaac tgtcaacttt cttgattttg aagaatttga
    12061 aggaaataag ttaataaaga atcaaatgat ggcttttttg aatgcaaaca atggaaattt
    12121 tattgcattt atgaaaagaa gccaagtgaa cgaatttatt gaaaaagtaa ccaattgtta
    12181 caatgacaaa ttaaatattt cggaagacga aaaagaaaga tttaaaaaaa atgctaaaca
    12241 aaaaaaagat ttgaaattgg agaaagaaga cgaaataagt gccgatgaac ttgatgatcc
    12301 ctgtatagtt ttttttaatc cccattctgg aatagaaatc gctgttggca ttaacagcgc
    12361 atttcccatg aaaagcaatc ccttctttca tccggagaaa agtgaaaccg atttttggca
    12421 gttattatta tctaattttt actcagctga attggtacaa ttctgtctgg atacctgcaa
    12481 aaataatttg ccgtttctca ggaaaaaggt atggaaaacc tatctgaagg acattgattt
    12541 tgtcttacgt tttttgaaag tcgattctta tcactcaaaa cctgaaatta catttttgtg
    12601 attttaaaac gaagagaaga gagatatttt cctcggaaaa taaatcacaa cgaaacaagc
    12661 ttcaatgtat taaatggagt ttggtttgct aacatattaa ttattaaacc ttagtacatg
    12721 acaaaaaaat tgtaaaaaca tttatattct ttattttcag acatttaata acgtaatagg
    12781 gctttgtctc aaactctact cacttttttg tcttgaaaca aataagtaag caaaaaaatt
    12841 tacttagttg aagcagctac gctcaacata aatcaaataa atttgatttt gttttcgctt
    12901 aaacgctgct ttcaagactg tgtccacttc actcgaaaaa cttaagctcg aaaagctgaa
    12961 atcgcccaaa ctcattccga cgaaatacgt cggagctcga ataaggacga tttttaaacg
    13021 cttttctcac ttgttttttg gctcaccgga caaagtcaaa cccaaaactt agcataattg
    13081 ttctgtttta cagatatata acataaaagt ctcaattttt gccatgtact aatatcaaac
    13141 gtataaatta ttgtatttat gaatggaatt gtgaacttga gccttgttcc tttaagggca
    13201 gaagacaacg aatgcagcga aatgatttct cagcttctgt ttggagaaac agttgaaata
    13261 atggaatgga aggaacgatg gctgcacatt cgcaatcttt cggacaattg ttgtggctgg
    13321 gctgacagaa aaatgattac tttgctttcg gaagatgaat acgaaaacct caagaataac
    13381 gaaaatttcc gcattctgaa agcggtttca ttttgtcgca atctgacatt gggaactact
    13441 tttctcctgc ccggaggaag cattctgaga aaatttcaga acgggaattt tacagatggg
    13501 acatatcaat atgccattca tccttccgat gctggaatgc caaaaccttt aaccggaggc
    13561 aatatcgttt ctttagctcg acaatatcat tttacaccct atttgtgggg cggaaaaagt
    13621 gcattgggaa ttgattgttc aggtttggtt caggttgttt tttcgctttg cggcgtcttg
    13681 ttaccgcgca atgcttctca gcaggccgaa atcggtgaaa aaataaattc tcttactcag
    13741 gctgaggccg gagatttggc tttctttgcc aattctgctc atcacattgt tcatgtgggt
    13801 attctaatga attctcacca aattattcat gcatcaggct gggtaaaaat cgaaaatatt
    13861 gatgaacatg gaattatttc cgaacaaaca ggaaactatt cccataccct ttactccatc
    13921 aaaagaatga tttaaatcat tttcaaaatc gtcagactct cttcaaaaaa aattctcctt
    13981 acctttaaaa aaaatcgagt atttgatgta agaaatcatc cggtttttcg tcattatagg
    14041 tgtatgaatc aaatttggta acttagtgag aattcagtaa tgtatggatg cagcacaatt
    14101 taattcggtt attttgccac ttgctgacca actgtttcgg gtggcaaaat ccattgtctg
    14161 caatgatgaa gctgccaaag atgccgtaca ggagttgaac gcaaaattgt gggaaaatcg
    14221 cagccaactg gataaagtgc aaaaccttac ggcttttgcc atgaagtcca tgcgcaattt
    14281 atgtctcgac tggttgcgaa caagaaaatc agattatgat gttcctctgg atgaaaatct
    14341 gatagcatct gcgcccaacc ctcatcagtt gatggaacaa aaagatctga caaactatgt
    14401 gaaacaattg atagacaaat tacccgaatt gcaaagaacc attattcacc ttcgcgatgt
    14461 ggaaggtttg gaaatttcgg aaatagcagc aattacttca atttcggaaa acgccataag
    14521 tgttaatctc tcgagagcac ggcaaaaatt aaggacagaa ttgatggaga aatcgaaaaa
    14581 ggaaaaaata tgaaggaaat tttgaataat cgttttatca atttattaaa aaacgacttg
    14641 caatgaaaga aattgatgat ttattaaata aatattttga aggaaaaact acccttgaag
    14701 aggaaaatta cctgaaatct tatttcagga atgaagaaat acaacccgaa catgaagtct
    14761 acaaaactct atttcatgtg tttgatgaaa aagccaaaga aaaaatacct caaccggttg
    14821 acgaaaaaaa tgcacaaccc aatgtgaaac ggttttggaa attaactgcc gtcattgcgg
    14881 gaattgctgc agcttttctg atgattattt gggttatgcc gatagagaaa aatcaggatt
    14941 ttgccattgt gaacggggaa aaaattgaaa atacacaatt cgctcaaaat tatgctttgg
    15001 aaaaattgaa ccatgtcagc tacaagttgg aaacaaattt aaaacctttg gaagcaattg
    15061 cgaccgttaa atcgagaatg gaaccggtga aaaaaattcc ggaaataaaa aaagaatgga
    15121 aaaaatagaa gaaacaattc atttaaacaa ttaacaatca cgaatatgaa aaagaatgga
    15181 atattaattt tgacaatttt gctgcttgca tctttccgag caggtgcaca aaccatcgat
    15241 aaattttttg atcgatatgc caatgacgat cgattcactt acgtttccgt cggcaaagga
    15301 atgatgaacc ttgcttcaac attcggagca aataaaaata tggacaagaa ccagaaggaa
    15361 gttttagacc gtttgacggg aatcaaaatt ttaactttgg aaaattcacg cgatgaaaaa
    15421 ttaatgaact cggttcttga tgaattggac aaggtaattc gggccgggaa atacgaaacg
    15481 gctgtcagag tgaaagataa ggatgaaacg gtgaacatct tcttcaggac aaccggcaag
    15541 gatcagtccg atatgattat tgtgtcaaaa gagaaaaacg aaatgtcgat aatatggatg
    15601 aacggaaaaa tgacaaaaga agaaatgtcc gatatttttt cgcaaaattc ctgaaaaact
    15661 tattttatat ttttgtttat taacccagat tactcattag ccaatttata aggaaagtca
    15721 aactctgaag atatgttcca taggctcgaa aaccaggctt gtttttttat ttaaagcgat
    15781 tttgagaaac aatttgtatt ttaagtttga gctaaaaaga tggtttccat ttattcatca
    15841 tccttttgaa gttaaatgca gctgctgcaa gcattacatt agtattatcg cctgtaatgg
    15901 ctcacctgca aaaacatgtg tctaagcata gatattagtc aatctgaaga gaaagaattt
    15961 aatatcgata actcctctca aagaagctct gaaggattta attttagcat tgaaactttc
    16021 ggcagaggca ttggtacttc tattgataaa gaaattaaga acttcttcgt gatgttcata
    16081 aactgtagca gctattgtat taaaagactt gaattggctt tcagccacat tattgtacca
    16141 tttagccaat gaaagtcttg cagcggcttt gatactgttt ttgttgtaaa tcattctcaa
    16201 ggagtgtgta agagcataac cctgtttcag gtctggatat tctctgaaaa gaatttttgc
    16261 acacaacttt ttatatcccg acaatttcgg atcactatat aaaaagatag tcaatacaaa
    16321 ctaaatcgaa gaccaagaga aaaattaatc tttaataccc ctaaaaatga gtttttcaaa
    16381 cattttattt aatttcgcac ttgttgcttg aatttactta taaacaaaat cgaaataaaa
    16441 taatgaattt gaaacaactc ttttgtaaac tgatgtttat tgcaattttc attctaatca
    16501 gtatttcgac attttcgctg aaagcacaac tttttcggga cagtattctg aaaaatgaag
    16561 aaaaaaccat ttatatgaat gaagttattg taacgggtac cagaagtgaa gcggatgtaa
    16621 gaaatttgcc catgagctta tccgtaatta ccgacaaaca aatacttgat cgccaaagcc
    16681 agtctgtaat tccgttactg aatgaactgg ttccgggact atttattacc ggtcgaggaa
    16741 ttatgggcta cggaatttcg acaggagctg ccggaacgat gaaaatgaga ggaattggag
    16801 gaaatcctac tacaggagta ttgatactga ttgatggaga accacaattt atgggcttaa
    16861 tgggacatcc catagcagac ttgtatcaat ctgtgatagc agatcggatt gaagtggtca
    16921 gaggcccggc ttctgctctt tacggatcca atgccatggg tggcgttatc aatattatta
    16981 catcaaaatt ttacaaggat accgttcaac aaaatttcca cttcggttac ggctcttaca
    17041 gca
//
LOCUS       DAOSCH010000041        16739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2369_length_16739_cov_6.588048, whole genome shotgun sequence.
ACCESSION   DAOSCH010000041 DAOSCH010000000
VERSION     DAOSCH010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16739
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2369_length_16739_cov_6.588048"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2584
                     /locus_tag="PLZ54_04685"
     CDS             <1..2584
                     /locus_tag="PLZ54_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOJ66260.1"
                     /translation="NSSADPLVVIDGIPQTIGLNDINPNDIASVEFLKDAASAAIFGS
                     RASNGVIVITTKSGDLNSKQKTIAEYRTSINYVTNKPKFIKGKELISLLDAAYYNRYP
                     ENKTLEDNNPIKYFPFSPDYANFLGFNRDWLNTYLLNNPLGTDWESALTQAAISQDVR
                     LSFQGGQKSNSYYISLSHKSDNELVDNKNKKRSTLVLKNDFKISERINTGFNINFTLN
                     TSKNSTFDKLESLFSRSSLLPIYAPDGSGKYFDARNINDKKGSNPLYRMSETWDDNIN
                     FNGLLLGYFDIKLMQKFAFRSELSMTPGTSRYRYFQSKDFYREDEAIDPSKSGIILYS
                     RTLSYGFNVNNLLNYNTKINNRHVIKVMLGNNIQSFNSDFNVARFEGFPTNYFQLTNA
                     NTEKVLTRQSAGMDGYRFVSFFNRTQYALDEKYFFELNARADGTSRFINDNRWGFFPG
                     LGMSWMLSEENFFSQIKSIDYLKFRTSYGFVGNAEVGNFPAESRVLNWAEYAGSPGFV
                     LDRIANLDVRWEKQLQLNVGFNISALKNKISASLDWFLKRNSDLLLNYNIGKFQGYFN
                     TEVPINSGNLNNNGFDLDLKTTNYDGLFKWKTEFNISMFNTKISKLSTQQNYIENGIN
                     RAYVGYPLGLYYLPLWAGVNPETGHEMIYEVIGPETNKTKTGKLLDAEMLTSVEYRNQ
                     SVLITDKTPYPKLYGGVSNFFAYKKFELSIHFSFQLGNWIYHNGMRQNSYVNTYDINN
                     KMVSLQNYWTPENKNSNTPLLYNSQMANRNSTRYLMNASYLRLRNLSMNYTFDSKNTK
                     KFGLGKIIAQINCQNLITLSKFSNGSPELSLQNAGAEANISLGNIGLNYGVATLSAGI
                     KLEF"
     gene            complement(3356..4402)
                     /gene="ilvC"
                     /locus_tag="PLZ54_04690"
     CDS             complement(3356..4402)
                     /gene="ilvC"
                     /locus_tag="PLZ54_04690"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HOJ66261.1"
                     /translation="MAVINFGGVDENVITREEFPLSKAREVLKDEVIAVIGYGVQGPG
                     QSLNLRDNGFNVIVGQRKGGKTWEKAVADGWVPGETLFDIEEAAERGTIIQYLLSDAA
                     QIEVWPRIKPYLTPGKALYFSHGFGITFKDRTGIIPPPDIDVIMVAPKGSGTSLRQLF
                     LEGRGLNSSYAIFQDATGKAFDRVIALGIGVGSGYLFETTFEREVYSDLTGERGTLMG
                     AIQGLLSAQYEVLRAHGHSPSEAFNETVEELTQSLMPLFAENGMDWMYANCSTTAQRG
                     ALDWMGPFHDATKPVFEKLYNEVASGREAQRSIDSNSRPDYRERLEEELKALRESEMW
                     RTGAVVRKLRPENN"
     gene            complement(4475..5206)
                     /locus_tag="PLZ54_04695"
     CDS             complement(4475..5206)
                     /locus_tag="PLZ54_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HOJ66262.1"
                     /translation="MGKEFSYIFKIAPSNVDFQGKLTLASLIELLMEAAGRHADGNGF
                     GMRQLNEFNASWVVLRIALEINYYPEQYDEIKISTWIEKIQPIKTIRNFCIENAQGEI
                     IASATSEWIMMDLTQRKPKDLLSLEGFEEFATGKEIPAGKLEKLPPVKGELVDKITVK
                     YGDIDINGHVNSIRYVQWLCNCFNLSDYREKQIERFEIHYISELHENENIKIFREEKK
                     KNDFYFEIDSDKKTTCKARISFSEK"
     gene            complement(5248..5820)
                     /gene="ilvN"
                     /locus_tag="PLZ54_04700"
     CDS             complement(5248..5820)
                     /gene="ilvN"
                     /locus_tag="PLZ54_04700"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HOJ66263.1"
                     /translation="MMEKTLYTVIIFSENTVGVLNQITVIFTRRGLNIETLSVSPSAI
                     AGIHKFTITVITTQDVIEKVVQQIDKRVDILKAYYNTDEDLVHQEIALYKVATDKLLE
                     LGSIEKMIRDYQIHVLELTENCVVFEKTGHYQETQKLFEELSSTIGVLQFIRSGRIAI
                     TKSKVERLSDMLEKMSKLEQKVNATTETQE"
     gene            complement(5884..7569)
                     /gene="ilvB"
                     /locus_tag="PLZ54_04705"
     CDS             complement(5884..7569)
                     /gene="ilvB"
                     /locus_tag="PLZ54_04705"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HOJ66264.1"
                     /translation="MKMTGAKALIESLLQEGVETIFGYPGGAILPVFDALYDYDRKVN
                     HILTRHEQGAAHAAEGYARVSGKVGVCLITSGPAATNAITGISDAMLDSVPLLVITGQ
                     VGASLLGTDAFQEIDVIGITQPITKWSYQIRRAEDVAWAVARAFYIASTGRPGPVVLD
                     FTKNAQSEEVDFQYKPCKFIRSYVPVPEVNFSQIEKAAELINAAKKPFAVVGHGVILG
                     QAEKDLMEFLEKADIPAAWTLLGASAMPSDFRLNKGFVGMHGNVAPNMKTNECDVLIA
                     IGMRFDDRVTGDLKTYASQAKIIHIDIDAAELNKNLKVEVPVLGSVKDVLPALTKLVK
                     PAKHTEWIDSFDIFEKTEYEKVIEREVFPKSGKITMGEVIHKVSEATHHNAICVTDVG
                     QNQMASARYFKFSQSRSLITSGGLGTMGFGLPAAIGAKIAAPDRTVCLFAGDGGFQMT
                     IQELGTIMENNIGVKMIVLDNKFLGMVRQWQELFYEERYSFTKMKNPDFVAIGKAYNI
                     DGKLVNEREELDEAIADMLKDDRPYLLVVNVETKGMVYPMIPAGTSVTNIILE"
     gene            complement(7651..9480)
                     /gene="ilvD"
                     /locus_tag="PLZ54_04710"
     CDS             complement(7651..9480)
                     /gene="ilvD"
                     /locus_tag="PLZ54_04710"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOJ66265.1"
                     /translation="MISRLRSTTSTSGRRMAGARALWRAAGMKNEQMGKPVIAIVNSF
                     TQFVPGHVHLHEIGQKIKAEIEKLGCFAAEFDTIAIDDGIAMGHDGMLYSLPSRDLIA
                     DSVEYMMNAHKADAMICISNCDKITPGMLMAAMRLNIPTVFVSGGPMEAGELDGVQLD
                     LIDAMVQSADDSISDEQIQRIENAACPTCGSCSGMFTANSMNCLTEAIGLALPGNGTI
                     VATHVNREKLFVQAAKLIVENAYKYYRDGDASVLPRSIATREAFLNAMKLDIAMGGST
                     NTVLHLLAVAHEGEVNFTMDDIDELSRHTPCLCKVAPNTQKYHIQDVNRAGGILGILA
                     ELSRGGLIDTNVRRVDGMTLKEAIHKYDITSPHVSAEIIQLYKSAPANRFNLVMGSQN
                     SYYKELDTDRTNGCIRDLDHAYTKDGGLAILKGNLAQDGCVIKTAGVDESIWKFSGPA
                     KVFSSQDAACEGILSGKVVAGDVVVINFEGPKGGPGMQEMLYPTSYIKSRHLGKECAL
                     ITDGRFSGGTSGLSIGHISPEAAAGGNIALLRDGDMIEIDIPARSIQVKLSEEELVKR
                     RQEELSRGKEAFTPKDRQRSVSKALKAYASMVSSADKGAVRLI"
     gene            10012..11904
                     /gene="dxs"
                     /locus_tag="PLZ54_04715"
                     /pseudo
     CDS             10012..11904
                     /gene="dxs"
                     /locus_tag="PLZ54_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
     gene            12027..13364
                     /gene="trkA"
                     /locus_tag="PLZ54_04720"
     CDS             12027..13364
                     /gene="trkA"
                     /locus_tag="PLZ54_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HOJ66266.1"
                     /translation="MKIIIVGAGEVGTHLAKLLARESMEITLSDENPDRLMDLESNYD
                     LLTSVGSSTSINHLREIGVENANLFIAVTPYESVNMTACMLATNLGAEKTLARIDNYE
                     YLFPENKDFFKKLGVDYLIYPEVLAAKEISESLKTSWLRQNMSFCNDALILLGIKVRS
                     NSEIINKKFSTGYFDHDKYRVVAIKRGMQTIIPKGSDEILDNDIVYFITTPQNLNFVR
                     EQAGKEDFPIRNVMFLGGSRIAQKTLKRLPDHIHTKVLEINNERSMELAEKFPDTLII
                     NCDGRNIDVLREEGIQEMDAFVAATGNSEMNLLACMVAKRFGVKKTIAEVENIDYIPL
                     AESMDIGTVINKKMIAASYIYQITLNASVLNVRNLTSADAEVVEFIAKAGSKITRSKI
                     KNLKLPENVNIGGIVRNGKGMIVSGETSIEPEDHVIVFCISSAIRKLEPFFNT"
     gene            13387..14856
                     /locus_tag="PLZ54_04725"
     CDS             13387..14856
                     /locus_tag="PLZ54_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017140927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOJ66267.1"
                     /translation="MNREINFKLVIRMLGSLLLSEGTALLLVFFISLIYRENDGVYFI
                     FTSLLCYVVGFAGFFFGKNAPMSVGKREGSLVVTSAWIIFSLFGSLPFWLSKSIPLFT
                     DAFFETISGFTTTGASILNNIEELSHSMLFWRSLTHWMGGLGIIVISLALLPMFGTSG
                     AQLFAAESSGPTKDKIHPKISETAKRLFVIYFLLTFSETILLRLGGMTWFDAVCHSFA
                     TIATGGFSTKQNSIQYWDSPYIQYTIIIFMILSGVNFSLYYFGFKNKLEKIRQNEELR
                     YYLIVLFGFAIIVALSMIDFSRPISFHKVEESWRNALFTVGSLMSTTGFTTVDYMQWK
                     PFVWVILLIVMLTGASAGSTSGAIKMVRIVIVFKFCYYEFKRLIHPKAVVPVRYSGKI
                     VFQDVVTRVLVFVLLYLIAAILGILILCMSGVNFIESVGGLVACLGGVGPGLGMVGPV
                     GNYSYFPVFAKWFLSFMMLLGRLELFTVLLLFTPAFWKK"
     gene            14948..>16739
                     /locus_tag="PLZ54_04730"
     CDS             14948..>16739
                     /locus_tag="PLZ54_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOJ66268.1"
                     /translation="MESFLYRIAQTYYNHYEDKISDFTFVFPNRRAGMFFQRYISQIA
                     KKPLFSPEILTINDCFTSVAQFHLADRLSLLFRLYRIYKEKSGTNENFDSFAYWGEML
                     LNDFDDIDKYRIDARQLFTNVTELKQIDRMFNYLSENQLDAIRQFWKNFIPIPKEKTQ
                     EDFIQIWKILYPVYQDFTEELKRENMATEGMIYRNLAERLESEEKIQEWEGKQFVFIG
                     FNALNRCEKTLFEVLQKKEKADFYWDYEAPALRDADNPASTFFMENTKTFPSKFSIET
                     PVEERKEQYFELIAVPSEIGQIKQIYEILNRIYPQDASVQQESWIKTAVVLPNENLLL
                     PALYSLPEHIEKVNVTMGFPLKSTPVSGLIEHVFDLQKRIRNSDEEVLFYHQPVTEIL
                     QHSYITLLCGDDSKNILQQMVENNSIYIRPEQLQINELFTAIFRPQNDAREMLTYLLN
                     ILRILRKNFQQIGFGNHQYRMETDFVIQYYRFLNRMSDILQYQSEGIEMKMDTLMRLI
                     FQYSAFITLPFVGEPLEGLQIMGVLETRGLDFDNILLTSFNEGVFPKNAPTHSFIPYN
                     LRRGFGLPAKEHQDAISAYNFYRLIHHAQKV"
BASE COUNT         5059 a         3230 c         3166 g         5284 t
ORIGIN      
        1 aaactcgtca gccgacccac ttgttgttat agacgggatt cctcaaacca ttggcttaaa
       61 tgacattaac cctaacgata ttgcttcagt agagtttcta aaagatgcag cctcagcggc
      121 tatttttggc tcacgtgctt ccaatggagt tattgttata accacaaaat caggtgattt
      181 gaattcgaaa caaaaaacaa ttgccgaata tagaacatca atcaattacg taacgaacaa
      241 accaaaattc attaagggca aagaactaat ttcattactt gatgcagctt actataacag
      301 gtatcctgaa aataaaacat tagaagataa caatcctatc aaatatttcc ctttttcacc
      361 ggattatgct aattttttag gttttaaccg cgattggttg aatacttatt tgcttaacaa
      421 ccctcttgga accgactggg aatcggcatt aacacaggct gcaatttctc aagatgtccg
      481 cttatcgttt caaggtggac aaaagtcaaa tagttactac attagtttat cgcataaatc
      541 ggacaatgaa ctagttgata ataaaaacaa gaaaagaagc actttagtat taaaaaatga
      601 ttttaagata tcggagagaa ttaacactgg atttaacata aatttcactc taaatacttc
      661 gaaaaattca acttttgaca agttggagtc tttattttca cgttcttcat tgctcccaat
      721 atatgcgcca gatggttcgg gtaaatattt cgacgcacgc aatataaatg acaaaaaagg
      781 cagtaatcca ctttatagaa tgagtgaaac ttgggatgac aatattaatt ttaatggttt
      841 attacttggt tatttcgata ttaaattgat gcaaaaattt gcatttagat ctgaacttag
      901 tatgacaccc ggtactagca ggtatcgata ttttcagtca aaagactttt atcgcgaaga
      961 tgaagctatc gacccatcaa aaagcggtat aatactatat tcaagaacct taagctatgg
     1021 atttaatgta aacaatttac tgaattacaa tacaaaaata aacaaccggc atgtcataaa
     1081 agttatgctg ggtaataaca ttcaatcctt taacagcgat tttaatgttg cacgctttga
     1141 gggttttcca accaattact ttcagctaac taatgcaaac accgaaaaag tactaacacg
     1201 acaatcggca ggaatggatg gctatcgatt tgtatccttt tttaacagaa ctcaatatgc
     1261 attagacgag aagtattttt ttgaattaaa cgcacgagcc gacggcacat ctcgattcat
     1321 taatgataat agatgggggt ttttcccggg gcttggaatg agttggatgt taagtgaaga
     1381 aaactttttt tcacagataa aatcaattga ttatttaaaa tttagaacaa gttatggttt
     1441 tgtaggaaat gcagaggttg gtaattttcc tgccgaatcg cgagtactca attgggctga
     1501 atacgcaggt agccctggct ttgtgttgga cagaatagca aacttggatg tgcgttggga
     1561 aaaacaacta caattgaatg taggattcaa tatttcagct ttaaaaaaca aaatatcagc
     1621 aagcctagat tggtttttaa aacgtaactc tgatttactt ttgaattaca atattggaaa
     1681 attccaagga tactttaaca cagaagttcc aataaattcg ggtaatttga ataacaatgg
     1741 tttcgattta gatttaaaaa ctacaaatta tgatggttta ttcaaatgga aaaccgaatt
     1801 taacatttca atgtttaata caaagatttc aaaactttcg actcagcaaa actatattga
     1861 aaatggaata aatagagctt atgtgggtta cccactaggt ttatactacc taccattatg
     1921 ggcaggtgtc aacccagaaa ctggacatga aatgatatat gaagttatag gacccgaaac
     1981 aaataaaact aaaacaggta aattattgga tgccgaaatg cttacatctg ttgagtatcg
     2041 caatcagtct gtattgataa cagacaagac accctatccc aaattatacg gaggagtgag
     2101 taatttcttt gcatacaaaa aattcgaact cagcatacac ttttcatttc aactcggaaa
     2161 ttggatatat cacaacggta tgcgacaaaa ttcatacgta aacacctatg acataaacaa
     2221 taagatggtt tcgctacaaa attattggac acctgaaaat aaaaactcaa acacaccact
     2281 tctttataac tctcaaatgg cgaaccgtaa tagcacgcgt tatttgatga atgcatcgta
     2341 tttgcgattg cgcaatttga gtatgaacta tactttcgac tcaaaaaaca ccaaaaaatt
     2401 cggacttgga aaaataatag ctcaaattaa ttgtcaaaac ttaataacat tatcaaaatt
     2461 ttcgaatggt tcacccgagt tgtcattaca aaatgcaggc gctgaagcaa acatatcgct
     2521 aggaaatatt gggcttaact atggagttgc tacattaagt gctggaatta aacttgaatt
     2581 ctaatacttt tcctctcatt tcatctcatc tcataatccc aaattgtcca ttagagctat
     2641 cgagtagaaa aatcgaatta aaaattcgat aattttttca aagttttcgt aaaaattatt
     2701 aacggttatt taaaaacaga ccaccgccta aatcataatt tctacaaagg aattacaggc
     2761 gataaaatgt aatgcttgca gcagctgcat ttaacttcaa gaggatgatg aataaataga
     2821 aattggctcg aatttaaaat aaaattgttg acttgataaa cgctatgttt taccaagtca
     2881 ataataattt aataaaaaaa tggacttttc aaggctcgac tgattatttt tcatgtttcc
     2941 gcctttcaaa aaagaaagac gcaggcttta gctttgtctt ttttacagaa ttaaatatca
     3001 ccgataatct gattatcaaa acaatacgat tatcttattc aaaatattaa cctaagattt
     3061 atagaggaaa gatatgataa tatgtaactt acaatttttt taacaaccaa tttgacttac
     3121 tagtcaaaat taggacattt ttcttaatca atttattctt ccttcggcga aaaataagat
     3181 tttaattttt gctccaactt tttaatttac acagttggtg aaatactgcc atgattgtca
     3241 tataaacaac atgaaaataa tataataaag ctaagcgggc ttttcataaa aaacctgtcg
     3301 tttgaattaa tttcaaaaca gacaggtttc tagtgaaatt cttaatcaaa aaaatttaat
     3361 tgttttcggg acgaagtttt ctaacaacag cgcctgttct ccacatttcg ctttcgcgca
     3421 aagccttaag ttcctcttca agccgttccc tgtaatcggg gcgggaattg gaatcaatgg
     3481 aacgttgtgc ttcacgacct gaagctactt cgttgtagag tttttcaaat accggtttgg
     3541 tggcatcgtg gaatgggccc atccagtcca atgcaccgcg ctgagctgta gtggaacagt
     3601 tggcgtacat ccaatccata ccgttttcgg caaaaagtgg catcagcgat tgcgtcagtt
     3661 cttccacagt ttcattgaaa gcttccgaag gagaatgtcc gtgtgctctg agcacctcgt
     3721 attgagccga caaaagtccc tgaatagctc ccatcaaagt tccgcgttca ccggtaagat
     3781 cggaataaac ttctctttca aaagtagttt caaataaata gcctgaacct acacctattc
     3841 cgagtgcaat cactctgtca aaggctttcc ctgtagcatc ctgaaaaatg gcgtaggaag
     3901 aattcaatcc acgtccttcg agaaacaatt gacgcaagga agttcccgaa cctttgggag
     3961 ctaccataat tacgtcaata tcgggaggag gaataatgcc cgtacgatcc ttaaaggtga
     4021 tgccaaatcc atgagaaaaa tacaaagcct tgccgggtgt cagatacggt tttatacgtg
     4081 gccaaacttc tatttgggct gcatccgaaa gcaaatactg aatgatggtt cctctttctg
     4141 cagcttcttc aatatcgaaa agtgtttcgc cgggaaccca gccatcggca acagcttttt
     4201 cccaagtttt accgcccttg cgttgtccaa caatgacatt aaaaccattg tcccgcaaat
     4261 tgagcgattg acccggacct tgaacaccgt aaccaattac ggcaattact tcatccttca
     4321 gtacttcacg agctttgctc aaaggaaatt cttcgcgggt tattacattt tcgtcaacac
     4381 cgccaaaatt aattactgcc atttttttgt agtttttaaa aattatttgt tattaagttg
     4441 tttgttatca attgtttgct aaaaatttta tttgttactt ttccgaaaag cttattcttg
     4501 ctttgcaagt cgttttttta tcactgtcta tttcgaaata aaaatcgttt ttcttctttt
     4561 cttctctaaa aatcttaata ttttcgtttt catgtaattc ggaaatatag tgaatctcga
     4621 agcgttcaat ttgcttttcc cgatagtcgg acaaattgaa gcaattgcat agccattgta
     4681 cgtaacggat actgtttaca tgtccgttaa tatcgatatc gccatatttc acggttattt
     4741 tgtctaccag ctcacctttc acgggaggaa gtttctccaa tttaccggcc gggatttctt
     4801 tccctgtggc aaattcttca aaaccttcca gagaaagcaa atcttttggt ttgcgttggg
     4861 tcaaatccat cataatccat tctgaagtgg ctgaggcaat aatttctccc tgtgcatttt
     4921 caatacaaaa attgcgtatg gtttttatcg gctgaatttt ttcaatccat gtcgatattt
     4981 ttatttcgtc atattgttca ggataataat ttatttccaa tgcaattcgt agcaccaccc
     5041 acgaagcatt gaattcattc aattgccgca tcccaaaacc atttccatcg gcatgtctgc
     5101 ctgctgcttc cattaaaagc tctattagcg aggctaaagt gagctttccc tgaaaatcga
     5161 cattggacgg agcaatttta aaaatatatg aaaactcttt tcccactcgt atttttaagt
     5221 tggtattgca acaatttgtc gttgcaatta ttcttgtgtt tcggttgtgg cattgacttt
     5281 ttgttcaagt ttactcattt tttcgagcat atcgctcagc cgttccactt tcgacttggt
     5341 gatggcaatt cgtcccgaac gaataaattg caaaacgcca atagtcgaac tcaactcttc
     5401 aaaaagcttc tgtgtttcct gataatgtcc ggttttctca aaaaccacac agttttcggt
     5461 gagttccagc acatgaattt ggtaatctcg aatcattttt tcgatagacc ccaattcgag
     5521 caatttgtcc gttgctactt tgtacaatgc aatttcctga tgtacaagat cttcatcggt
     5581 attgtagtaa gccttcagaa tatcgacgcg tttgtcgatt tgctgcacta ctttttcgat
     5641 aacatcctgt gtggtaatga cggtaatcgt aaatttatga ataccggcaa tggccgatgg
     5701 agaaaccgac aaagtctcaa tattcagacc tctacgcgtg aagatgacgg taatttggtt
     5761 cagtacgcca accgtatttt ccgaaaaaat gataacggta tataatgtct tttccataat
     5821 gcaaagtttt aaaaaatgct gataaatctc tgaagttata tttttgaaat ttcagtaata
     5881 aatctactcc agaataatgt tggttacact tgttccggca ggtatcatcg ggtataccat
     5941 tccttttgtt tctacattta caaccaacag ataaggtctg tcatccttca gcatatcggc
     6001 aatggcctca tcgagttctt cccgttcgtt aaccaatttt ccatcaatat tataagcctt
     6061 tccaatagct acaaaatcgg gatttttcat tttggtgaaa gagtagcgtt cttcataaaa
     6121 taattcctgc cactgacgca ccatgcccaa aaacttattg tcgagcacaa tcatttttac
     6181 gccgatattg ttttccataa tggttccaag ttcctgaata gtcatttgga aaccgccatc
     6241 gccggcaaac agacaaaccg ttctgtccgg agcagcaatt tttgcgccaa tagctgccgg
     6301 aagtccaaaa cccattgtgc ccagtccgcc tgaggttatc aaacttctgg attgtgaaaa
     6361 tttaaaatag cgagccgatg ccatttgatt ttgccccaca tcagtaacac aaatagcatt
     6421 gtgatgagtg gcttcggata ctttgtggat aacttctccc attgtaattt ttccgctttt
     6481 ggggaatacc tcgcgttcaa tcactttttc gtattccgtt ttttcaaaaa tatcaaagga
     6541 atcaatccat tcggtatgtt tggcaggttt aacaagtttt gttaaagccg gtagcacgtc
     6601 ttttaccgag cccaaaactg gcacttccac tttcagattt ttattcagtt cagctgcatc
     6661 aatatcgata tgaattattt tggcttgact ggcatacgtt ttcagatcgc ctgtaacacg
     6721 gtcgtcgaag cgcataccga tggcaattaa tacatcacat tcattggttt tcatattggg
     6781 agctacattt ccatgcatgc ctacaaatcc tttgttcagc cgaaaatctg atggcatggc
     6841 cgaagccccc agtaaagtcc atgcagccgg gatatcggct ttttccaaaa attccatcaa
     6901 gtctttttct gcctgaccga gaatcacgcc atgaccaacc acagcaaaag gttttttagc
     6961 ggcattaatc agttcggctg ctttttcgat ttgggaaaaa ttaacttctg gaaccggaac
     7021 atagctgcgg ataaatttac aaggtttgta ttgaaaatct acttcttccg actgtgcatt
     7081 tttggtaaaa tcgagtacta caggtccggg acgtccggtg gatgcaatgt aaaaggcgcg
     7141 agcaactgcc catgccacat cttcggcccg ccgaatctga tatgaccatt ttgttatagg
     7201 ttgtgtaata ccaatcacat caatttcctg aaatgcatcc gtaccaagca aacttgctcc
     7261 aacttgtcct gtaatgacta acaatgggac gctgtcgagc atagcgtcgc tgattccggt
     7321 aatagcatta gttgctgccg gtcccgaagt tatcaggcaa actcccactt tccccgaaac
     7381 gcgggcatat ccttcagctg catgagcggc accctgttcg tgacgcgtaa gaatatgatt
     7441 tactttccga tcatagtcgt ataaagcatc aaaaacgggc aaaatggcac cgcccgggta
     7501 tccgaatatg gtttctactc cttcttgcag caatgattct atcaatgctt ttgctcctgt
     7561 cattttcata ttattattcc tccttttttc atttgggaat ctttgaatca aataataaac
     7621 tttttgtact gtaaaaaatc atttttcatt ttaaatcaac cgcactgcgc ctttatcggc
     7681 cgaacttacc attgaagcat aggcttttag agcttttgaa accgaacgtt gacggtcttt
     7741 cggcgtgaaa gcttcttttc ctctcgacaa ctcttcctgg cggcgtttta ccaattcttc
     7801 ttcactgagt ttcacttgta tggaacgtgc gggaatatcg atttcaatca tgtcgccatc
     7861 gcgcaataat gctatattgc ctcctgctgc ggcttcaggc gaaatatgac ctatggaaag
     7921 ccccgaagtt ccgccagaaa accttccgtc ggtaatcaat gcacattctt ttcccaagtg
     7981 gcgcgatttg atataggaag tcggatagag catttcctgc attcccggcc ctccttttgg
     8041 tccttcaaaa ttgatgacta ccacatcacc tgccacaact tttccactta aaattccttc
     8101 gcaagctgca tcctgagaag aaaatacttt tgccggaccg gagaatttcc agatactttc
     8161 atctacgccg gccgttttaa taacacatcc atcctgagcc aaatttcctt tcaaaattgc
     8221 aagtccgcca tcttttgtat aagcatgatc caaatcacga atacatccgt ttgttctgtc
     8281 ggtgtccaat tccttataat aagaattttg agaacccatt accaaattaa accggttggc
     8341 cggtgcactt ttatagagct gaatgatttc agccgaaaca tggggcgatg taatatcata
     8401 tttatgaatg gcttccttta aagtcattcc atcaacgcga cgaacattgg tgtcaatgag
     8461 tcctccacga ctgagttctg ccagtattcc caaaattcca cctgcacgat tgacatcctg
     8521 aatgtggtat ttttgagtgt ttggtgctac cttacataaa caaggtgtat ggcgggaaag
     8581 ttcatcaata tcgtccattg taaaatttac ttcgccttcg tgagcaacag ccagcaagtg
     8641 caaaacggta ttggtagagc ctcccatggc aatatccagt ttcatggcgt taagaaaagc
     8701 ttcacgtgtt gctatggaac gaggcagaac agaagcatcc ccatcccgat agtatttata
     8761 agcattttca acgatcagtt tggcagcttg tacaaacaat ttttcccggt tgacgtgagt
     8821 agctactatt gttccgttac ccggaagtgc cagtccaata gcttcggtca gacagttcat
     8881 cgaattggcg gtaaacattc ctgaacaaga accacaagta ggacaggccg cattttcaat
     8941 tctttgaatt tgttcgtcgg aaatcgagtc gtcggccgac tgtaccattg catcaatcaa
     9001 atcgagctgt acaccatcca attcgcctgc ttccattggc ccacccgata caaaaacagt
     9061 agggatattc aatcgcatgg ccgccattaa cataccggga gtaattttat cgcaattgct
     9121 gatacatatc atggcatcag ccttgtgagc attcatcatg tattccacgc tatcggcaat
     9181 caagtcgcgg gaaggtagcg agtaaagcat tccgtcatgc cccatggcta ttccatcatc
     9241 aatggctatg gtatcaaatt ctgctgcaaa acagccgagt ttttctattt cagcttttat
     9301 tttctgacca atttcgtgta agtgaacgtg tcccggaaca aactgagtaa aagaattgac
     9361 tatcgcaatc acgggttttc ccatttgttc gtttttcatg ccggcggctc gccacaaggc
     9421 tctcgctccg gccattcttc ttcctgatgt gctggttgta cttctcaacc tgcttatcat
     9481 tgattgttct tcaacactgt ttattgaagt tcccattcaa aaattatttt aaaaatttat
     9541 attacttact gattttaaat caattatttg tttgcttttt actttttcta agtaaaaaag
     9601 taaattcaat aaaaacagaa aaacctttct ctatgaaggt gagaaaggtt ttatctgtta
     9661 tgctttatta tataaaaaat aataagaacc atcctcacct ttgattgtgg accaaaagta
     9721 gcacgacgat tcctaaaaaa ttatttttcc gatacatttc ttcaaaattg aatttcggtg
     9781 caaagataga tgaaaaaatt actttatcaa aaaaaatcat attttttttc aatttgttat
     9841 ataaggcagt cggaaataat taaaaaataa ttttatagct ttcaattatt ctgtgtataa
     9901 acttgtcttt tttataaagg cggtataaaa acaaagcaat aaattatttg tttaaaaagt
     9961 agcaaacatt aaaaaatgta ttaattttgt gcacaatcat aatctcagaa aatggaaaat
    10021 aaaaacttta cctatatatc tcacattgaa tcatctaagg atttgaaagt tcttaaaaaa
    10081 gaagaactta aagcggtttg ccgagaatta agaacactta ttatcgacga agtttcccaa
    10141 catccgggtc atttggcatc cagtcttggt gccgtggaac tcacggttgc tctacattat
    10201 gtatttaata ctccctacga ccgcattgtt tgggatgtag ggcatcaggc gtatgcgcac
    10261 aaaattctta ccggtcgtcg tgataaattt cacaccaatc gtgaatttaa aggaattagt
    10321 ggcttccctt ctcccaaaga aagtgaatat gatgcttttg gagttggtca tgcttcaact
    10381 tccatttcag catcattggg aatggatatt gcttccaaac tgaaaaatga aaaagacagg
    10441 catgtcgtag ccgtcattgg tgatggagct cttaccggcg gacttgcttt tgaagggttg
    10501 aacaatgtgt caatgagtaa aaatatcaat gacttgttga taattgttaa cgataatcag
    10561 atggctattg accccctgaa aggtggtatt accgaatact tgattgatat tactacttct
    10621 cagacctaca acaaaataag gtacaaaatt tataaaatgc tgaaagggtt gaatttgatt
    10681 gatgagtcga gaaaagacaa tatcattcgt ttcaacaatg cgctgaaagc tacccttacc
    10741 cgacagcaca atctttttga aggattgaat attcggtatt tcggaccggt ggatgggcac
    10801 gatgttaatg aattggtgag aattctctca gaaattaagg attataaggg ccctaaaatt
    10861 ttgcattgtc taaccaaaaa aggaaaaggt tacgagcctg ccgaaaaagc tgtaaccgaa
    10921 tggcattcac cgggaaaatt ctgtgtggaa acaggagaaa aaacttcttc caattctgtt
    10981 tccgagcctc ctctttttca gcatgttttc ggagaaaccc tgctggaatt ggctaaaagc
    11041 aacgatagaa ttgtaggggt ttcacctgcc atgctcagtg gttgttccat gaacatcatg
    11101 caaaaggaat ttccggaaag agtatttgat gtaggtatag ccgaaggcca tgccgtaact
    11161 ttttcggccg gattggcgaa agaaggaatg attcctttct gtaacattta ttcgactttt
    11221 tgccaacgag cttatgacaa tgtgattcac gatgtagcat tacaaaattt gcatgtagtg
    11281 atttgtctcg atcgagcggg attggtgggt gcagatggcc ccactcatca cggtgtattc
    11341 gatttggcct atatgcgttg tattcctaat atgaccattg ccgctcctcg aaatgagatt
    11401 gaattgcgca atttgatgtt tacggcgcaa caacccgact tgggaccgtt tgtaattcgc
    11461 tatcctcgtg gagccggatc tgttgtcgat tggagaaaac caatgcagag gcttgaagta
    11521 ggtaaaggtg aatgtttgaa agagggtgaa aatttggctg ttttaactat tggaacaatg
    11581 gcagtgaaag ccgaaaaagc tattgagaaa gttgaaaacg agtgccattt gtcaattgca
    11641 cattatgaca tgcgttttct taaacctttg gatgaaaata tgctccataa aatcggagct
    11701 aaattttcat acgttttgac tattgaggat ggcgttatac aaggggggct tggcagtgca
    11761 gtgctggaat tttttgccga tcataattat tcggttcggg tgaaaagact tggcatacct
    11821 gacaatttta ttgaacaagg ttcgcctgac gaattatatg ccatgctgga tatggacgaa
    11881 aatgggatat caaaaaccat tatcgacatg ctacaaaaaa acactgaaaa cgataatcca
    11941 aaaaaacaac agatagtttc ttccaataat tttggaaaaa ataaaaattc tataatcaac
    12001 agaattttca gaaaagattt caaagaatga aaattattat tgttggggca ggagaagtcg
    12061 gaacgcactt agcaaaattg ctggctcggg aaagtatgga aataacccta agtgatgaaa
    12121 atcctgaccg gctgatggat ttggaaagca actacgattt actcaccagt gtgggttctt
    12181 ccacttccat caatcacttg agggaaattg gcgtagaaaa tgccaatttg tttatagctg
    12241 ttactcccta tgaaagtgta aacatgactg cctgcatgtt ggccaccaat ttgggtgccg
    12301 aaaaaacgct tgcgcgtatt gataattacg aatatctttt ccccgaaaac aaggattttt
    12361 tcaagaaatt gggggtcgat tatttgattt atcctgaagt gcttgccgca aaagaaattt
    12421 cggaatcatt gaaaaccagt tggctacgac aaaatatgag tttttgcaat gatgctctga
    12481 ttttgctggg tattaaggtt cgaagcaatt cggaaattat caacaaaaaa ttcagtaccg
    12541 gttattttga tcatgacaag tacagagtag tcgccataaa acgtgggatg cagaccatta
    12601 tacccaaagg cagcgacgaa attttggaca atgatattgt gtattttatc actacacctc
    12661 aaaatttaaa tttcgtaagg gaacaagcag gcaaggaaga ttttcctatt aggaacgtta
    12721 tgtttttggg tggcagccga attgcacaaa aaacattaaa aagacttccg gatcatattc
    12781 ataccaaagt tttggaaata aataatgaaa ggagtatgga gttggctgaa aagttcccgg
    12841 atactctgat tattaattgc gatggtcgaa atattgatgt ccttagagaa gaaggaattc
    12901 aggaaatgga tgcttttgtg gcagcaacag gaaattctga aatgaatttg ttggcatgta
    12961 tggttgccaa acgttttggt gtaaaaaaaa cgattgcaga agttgaaaac attgattata
    13021 tcccacttgc agaaagcatg gatataggca cggttatcaa taaaaaaatg atagctgcca
    13081 gttatattta tcaaatcact ttaaatgctt ctgtcttgaa tgtcagaaat ttaacttctg
    13141 ccgatgctga agttgtggaa tttattgcca aagcaggttc aaaaattaca cgttctaaaa
    13201 taaagaattt aaaactgccc gaaaatgtaa atatcggtgg tattgtgaga aatggaaaag
    13261 gaatgatagt cagtggagag acaagtatag aacccgaaga tcatgtgata gttttttgta
    13321 tttcttcagc tattcgtaaa ctcgaaccct ttttcaacac ctgaccccat tttattcact
    13381 aaaaccatga atcgtgaaat aaattttaaa ctggtgattc gtatgttggg cagtcttcta
    13441 ctgagtgaag gaactgcttt attgttggtg ttttttattt cattgattta cagggaaaat
    13501 gacggtgttt attttatttt cacctctttg ctttgttatg ttgtcggctt tgcgggtttc
    13561 ttttttggaa aaaatgctcc tatgtctgtt ggtaaacgcg aaggttcgct ggtggtaact
    13621 tcggcatgga taattttttc attgtttgga tctcttccct tttggcttag taaaagcatt
    13681 cctcttttta cagatgcctt ttttgaaaca atttcgggat ttacaacaac cggcgcttcc
    13741 attttaaata atattgaaga actgtcccac tccatgctgt tttggagaag tcttacccac
    13801 tggatgggtg gtttgggcat catcgttatt tctttggctt tgctgcctat gtttggcaca
    13861 agcggggcac aactttttgc agccgaaagc agtggcccta cgaaagataa aattcatccc
    13921 aaaatcagtg aaacagctaa aagattgttt gtaatttatt ttttgctcac tttttccgaa
    13981 actattttgt tgcgattggg aggaatgact tggtttgatg ccgtttgcca ttcgtttgcc
    14041 accatagcta caggcggatt ttcaacaaaa caaaacagta tccaatattg ggattcgcct
    14101 tacattcaat acaccataat tatttttatg attttatccg gtgtcaattt cagcctttat
    14161 tatttcggat ttaaaaataa gctggaaaaa atacgccaaa atgaggagtt gcgttattat
    14221 ctgattgtat tgtttggctt tgctattatt gttgcacttt ccatgattga cttttctcga
    14281 cccatatctt ttcacaaagt ggaagaatca tggagaaatg ccttattcac agttggttca
    14341 cttatgtcaa ccacaggttt tactaccgtt gattatatgc aatggaaacc ctttgtgtgg
    14401 gtaattttgc tgattgtcat gctgacgggt gcttcggcag gttctaccag tggggctatt
    14461 aaaatggtgc gtatcgtgat tgttttcaaa ttttgttatt acgaattcaa aagacttatc
    14521 cacccaaagg cagtcgttcc ggtgcgttac agcggaaaaa ttgtatttca ggatgtggtt
    14581 accagagtgc tggtttttgt tttgctttat ttgattgcag ccattctggg aatcttaatt
    14641 ctttgcatgt cgggtgttaa ttttattgaa tcggttggcg gacttgtggc ttgtttgggc
    14701 ggcgttgggc caggcttggg aatggttggg cctgtgggaa attattctta tttcccagtt
    14761 tttgccaaat ggttcctgtc gtttatgatg ttgttgggac gtcttgaact tttcacggtt
    14821 ttgctgcttt ttacaccagc gttctggaaa aagtaatttt tcgatgaaat taaaatttta
    14881 atctcagctg cttttgttgt aattttggta aattcaacca aagcaggttt tattttatta
    14941 ttcaacaatg gaatcttttc tctatcgtat tgctcaaaca tattacaacc actatgaaga
    15001 taaaatttcc gattttacct ttgtctttcc caacaggcgc gcaggcatgt tttttcagcg
    15061 ctacatttcg caaatagcca aaaaaccgct tttctcgccc gaaatattga ccattaacga
    15121 ttgttttacc tctgtagccc aatttcattt ggctgacagg ttgagtctcc tgtttcgtct
    15181 gtatagaatt tataaggaaa aaagtggtac taacgaaaat ttcgattctt ttgcttattg
    15241 gggcgaaatg ttgctcaacg acttcgacga tatcgacaaa tatcgcattg atgcccgcca
    15301 actgtttacc aatgttactg aactgaaaca gattgaccgc atgttcaatt atctttccga
    15361 aaatcagttg gatgctattc gtcagttttg gaaaaacttt atcccgattc ctaaggaaaa
    15421 aacgcaggaa gattttatac aaatttggaa aattctttat cctgtttatc aagattttac
    15481 ggaggaattg aagcgggaaa acatggcaac ggaaggtatg atttatcgca acctagccga
    15541 acgactcgaa agcgaagaaa aaattcaaga atgggaagga aaacaatttg tttttatcgg
    15601 attcaatgcg ctgaatcgct gcgaaaaaac tctttttgaa gtcctccaaa aaaaagagaa
    15661 agccgatttt tactgggatt atgaagctcc ggctttacgc gatgccgaca atccggcctc
    15721 aacttttttc atggaaaata ccaaaacttt tccttccaaa ttttccattg aaactcctgt
    15781 cgaagaaagg aaagaacagt attttgagct gatagccgtg ccttctgaaa ttggtcagat
    15841 caaacagatt tatgaaattc taaatcgcat ttatcctcag gatgcatccg ttcagcaaga
    15901 aagttggatt aaaacggctg ttgttttgcc aaatgaaaat ttgctcttgc ctgcgcttta
    15961 ctccctgccc gagcacatcg aaaaggtaaa tgttactatg ggttttccgc tcaaaagcac
    16021 ccctgtttcc gggttgattg agcatgtttt cgacttgcag aaacgaattc gcaattccga
    16081 tgaagaagtg ttgttttatc atcaaccggt aaccgaaatt ttgcagcatt cctacattac
    16141 gttgctttgt ggagacgaca gcaaaaatat acttcagcaa atggtagaaa acaactcgat
    16201 atacattcgg cctgaacaac ttcaaatcaa tgagttgttt actgctattt ttcggccgca
    16261 aaatgatgca cgcgaaatgc tgacgtattt gttgaacatt ttacgtattt tacggaaaaa
    16321 ttttcagcaa attggttttg gaaatcatca atatcgaatg gaaaccgatt ttgtgattca
    16381 atattatcgt tttctaaacc gcatgtccga tattttgcaa tatcagtcag aaggaatcga
    16441 aatgaagatg gatactttga tgcgattgat ttttcaatat tctgccttta ttactttacc
    16501 ttttgtcggt gaaccactgg aaggtttgca aattatgggc gtgttggaaa ccaggggttt
    16561 ggattttgat aatattttgc tgacttcttt caatgaaggt gtttttccaa aaaatgctcc
    16621 cacccacagt tttattccct acaatttgcg tcgtggtttc ggcttgcctg caaaggaaca
    16681 tcaggatgcc attagtgcct acaattttta taggctgatt catcatgccc aaaaggttt
//
LOCUS       DAOSCH010000042        16682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2380_length_16682_cov_5.718350, whole genome shotgun sequence.
ACCESSION   DAOSCH010000042 DAOSCH010000000
VERSION     DAOSCH010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16682
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2380_length_16682_cov_5.718350"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="PLZ54_04735"
     CDS             <1..1100
                     /locus_tag="PLZ54_04735"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HOJ66269.1"
                     /translation="KPNNMIKVGDKVRFLNSTGGGIVTRVDEKEKIVYVSDADGFEIP
                     MLERECVVIPEVNENTNFPKKDFKSAPAAFISENESPAAKSINIQPKPEITKEEIFET
                     PEGENLKVLLAFVPEDIKQLQTTSNDFYLVNDSNYFLFYNIALFENEWCKSVANGLIE
                     PNMQEWIAEIKKEELNAWEKIRVQIIPFKKDKTYRPQQVIDIDLKVNLMKFYKLHSFG
                     ENDYFDVPAMMLNPLEEMETHQLQNISTEEIKQAISQKEKPERRPRISKPANKLEIVE
                     VDLHIHQLVDTTVGMNNTDMLKLQLEKFESVLNEYKNRKGQKIVFIHGKGEGVLRNEI
                     EKLLRTKYKNYYFQDASFQQYGFGATMVTIR"
     gene            1350..2237
                     /locus_tag="PLZ54_04740"
     CDS             1350..2237
                     /locus_tag="PLZ54_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66270.1"
                     /translation="MDQLSFKRYFLFVFSLILSVSFFSCGEKEENEPDITNSIYVTQV
                     FEYVYAPGQHAQLAKPADIEHFIGEPSDEKGFLYLGGFGGYVIAGFDHDIQNKDGYDL
                     EIFALPGASPEPAIVYVMSDDNGDGQPNEIWYELKGNQFENEKRNYWVRYYKPSSSND
                     NIRWKDSEGESGELISGFGALNTSSWWWPTCMEDSITFEGSRLPDAYDNKSSESAQSW
                     VVPTDRFIWGYAENNFGIDYDTHHHSNKLDISNAVDSSGKAVYLEKIRFIKIQTAVFQ
                     RAGWVNEVSAEIRGAAELK"
     gene            2367..3809
                     /locus_tag="PLZ54_04745"
     CDS             2367..3809
                     /locus_tag="PLZ54_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOJ66271.1"
                     /translation="MENLEPSVVWKFFGEITQIPRPSKKEEKIREYLLDVAQQYRLEA
                     KTDQAGNVLICKPATFGYENHNKIILQSHMDMVCEKDGDVQHNFESDPIQTFIDGDWI
                     KAKGTTLGADNGIGMAMMLAVLTSDNISHPALECLFTVDEETGLTGASAIEDHFLSGQ
                     ILINLDSEDDGVIFIGCAGGIDTTINFPFIAEKSPNNYFSFSLTVKGLKGGHSGDDID
                     KRHGNAIKILNRFLWNVNKKLDLRIHTFRGGNLHNAIPREAEVVASVPFEDKEKLRVL
                     LNEFIAEIEGELKEDEPALQILISSGEIPDQVLDKKSSDKLLNALYACPHGVKAFSRD
                     MPNLVETSTNLASVRMQENNSFLIITSQRSSVESAKFDIAHQVESVFTLAQAQATHGE
                     GYPGWKPNLNSEILQKAKVCYKNITCEEPKVRAIHAGLECGLFLKKYPYLDMISIGPQ
                     MYGVHSPNERLSISSTRKCWQWLLKILEKS"
     gene            3811..5079
                     /locus_tag="PLZ54_04750"
     CDS             3811..5079
                     /locus_tag="PLZ54_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOJ66272.1"
                     /translation="MTNISGNNSLGESRIQPNKLKIINDPVFGFINIPDDFLYEIIQH
                     PYFQRLNRIKQLGVSFYVYPGAQHTRLQHSLGAMHLMGEAIEELRQKGHVISSEEEQA
                     ARACMLLHDIGHGPFSHALENSLVKGIHHEEISIMLMQQLNKEMNNNLALALEIFSGH
                     YPKKFLHQLVSGQLDMDRMDYLSRDSFFCGVSEGIIGSARIIKMLNVFHGELVVELKG
                     IYSIEKFLVARRLMYWQVYLHKTLVATEKMLTNILKRAKELASVGETLFASPALHYFL
                     YHHLDKQNFVRSTEALDYFVQLDDSDVICAIKVWAHHPDKVLSFLCNAFINRKLFKVE
                     MLQEPLDETKKEELLKKYSRYFDVSQQEAEYFMGEEIVSTDTYDPVDEKINILYKDGT
                     VKDIADASDMLNINVLTKKVYKNYFCYCRI"
     gene            5194..6246
                     /gene="lpxD"
                     /locus_tag="PLZ54_04755"
     CDS             5194..6246
                     /gene="lpxD"
                     /locus_tag="PLZ54_04755"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOJ66273.1"
                     /translation="MEFTAQQIADFLGGQVEGDPSVKVSNFSKIEEGKPGTITFLSNP
                     KYSHYIYDCNASIVLVNKDFKPEKEIPATLIRVNNAYECLAQLFSLVEQSKPKKKGIS
                     PLAFIASTAQIGEDVYIAPFVYVGENVKIGKNTQIHSHSNIEDNSSVGENTILFSGVH
                     VYPDCSIGNNCILHSGVVIGADGFGFAQSEDGTYKKITQIGNVVLEDDIEVGANTTID
                     RATLGSTIVHKGVKIDNLVQIAHNDEIGENTVIVAQTGIAGSTKIGKNCVIGGQVGIV
                     GHLQIADNTQIGAQSGVANSVKTPGQIIIGSPAIPIQNFRRSSIVFKNLSDLQKMVIE
                     LQNKVEELEKTKTSEK"
     gene            6306..7688
                     /locus_tag="PLZ54_04760"
     CDS             6306..7688
                     /locus_tag="PLZ54_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008759880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOJ66274.1"
                     /translation="MTRQRTLKESFTLEGKGLHTGLDIVITFLPAPENHGIKLKRIDL
                     EEPVIIEALAENVTHTVRGTVLQKGDVQVSTVEHALAALYAFGIDNCLIEVNAPEFPI
                     LDGSARFYAEKIKEIGIIEQKAEKDFYVVRKKIEYSLPDSNSKITLLPDDVFSVDVHI
                     GFDSAVLANQYASLDSLDKFADEIASARTFVFVHEIEPLLSMNLIKGGDLKNALVIYD
                     KELSQDTMDRLTDKLHQPRIDATKLGYINGELNYPNEPARHKLLDVIGDLSLIGKPLK
                     AKVIVQYPGHKINTNLAKIIRKEIKKQEVSAPVYDDSVPPVLDINAIRKLLPHRWPFL
                     LVDKIIDINERVIVGVKNVSGNETFFMGHFPNEPVMPGVLLVEAMAQTAGILVLNKVE
                     EPEKYSTYFMKIDNVKFRQKVVPGDTVIFRLEMMDEIRHGCTDMKGYAFVGGKIVAEA
                     QFMAQIIKNK"
     gene            7713..8492
                     /gene="lpxA"
                     /locus_tag="PLZ54_04765"
     CDS             7713..8492
                     /gene="lpxA"
                     /locus_tag="PLZ54_04765"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOJ66275.1"
                     /translation="MKQPLAYIHPDAKIAPSVVIEPFVTIDKNVEIGEGTRIGPNVTI
                     LEGARIGKNCTIFPGAVIAAIPQDLKFKGEDTLAIIGDNTTIRECVTVNRGTASKGKT
                     QIGDNCLIMAYCHIAHDCFVGSNVIMSNATQLAGEVFIDDYAVISGGVLVHQFTHIGS
                     HVIIQGGTKVNKDVPPYVKAGREPLSFEGINSVGLRRRGFTNEQIGFIQEIYRYIFLS
                     KMNVSNAIDRIEAELPATKERDDILLFIKNSQRGIIKSFLE"
     gene            8548..10239
                     /locus_tag="PLZ54_04770"
     CDS             8548..10239
                     /locus_tag="PLZ54_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010803554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOJ66276.1"
                     /translation="MKINEEETPKKNLNFIEAIVDQDVKEGKNGGRVQTRFPPEPNGY
                     LHIGHAKAICLNFDIAKRYGGICNLRFDDTNPVKEDVEYVDSIMEDIQWLGYQWKNVY
                     YASDYFQKLWDLAVLFIKKGLAYVDEQSAETIAKQKGTPTVPGIESPYRNRPIEENLA
                     LFQKMTNGEIPEGGMVLRAKIDMASPNMHFRDPIMYRIITSHPHHRTGWQWKVYPMYD
                     FAHGQCDYFEGVTHSLCTLEFEVHRPLYDWFIDQYQDTDYRPRQIEFNRLNLTYTVMS
                     KRKLLQLVQEKFVSGWDDPRMPTLCGLRRRGFTPESIHNFIDKIGYTKFEALNDIGLL
                     EHAARESLNKTAMRVNAVLHPVKLIVTNYPEGKVEMMTTQNNPEDPEAGTREVVFSRE
                     LFIERDDFMENPPKNYFRLMPGQEVRLKSAYIVKCTGCKKDENGVVTEVYCEYDPMTK
                     SGMPESNRKVKGTLHWVSAAHCVEAEVRLYDRLFNVENPSADERDFRELLNPDSLQIL
                     TGCKLENSLKDAKPLEHFQFQRIGYFTVDLDSTPDKLIFNRTVSLKDSWSKELNK"
     gene            10925..11500
                     /locus_tag="PLZ54_04775"
     CDS             10925..11500
                     /locus_tag="PLZ54_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOJ66277.1"
                     /translation="MDFSLIQIASAFMVLFAIIDILGSVPIILAIKSKGENVHAGKTS
                     LVALVILVVFLFAGDWILQLFNVDIESFAVAGSFVIFIFSLEMILDVEIFRNKGPEGA
                     SSVVPIAFPLIAGPGSFTTLLSLRAEYAIENIIIALVLNICFVFFVLRFTSKIEKWMG
                     KGGIYILRKFFGIILLAVSVKLFTTNIRFLL"
     gene            complement(11563..12729)
                     /locus_tag="PLZ54_04780"
     CDS             complement(11563..12729)
                     /locus_tag="PLZ54_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOJ66278.1"
                     /translation="MKNQRYDLRGVSASKEDVHNAIRNLDKGIFPKAFCKIIPDILGN
                     NPDYCNIMHADGAGTKSSLAYAYWKETGDLSVWKGIAQDALIMNTDDLLCIGAVDNIL
                     VSSTIGRNKNLIPGEVVSAIINGTDELIQQLGEMGVKLYFTGGETADVGDLVRTIIVD
                     STVTCRMKRADVIDNANIRPGDVIVGLSSSGQASYEKEYNGGMGSNGLTSARHDVFAH
                     YLAEKYPECYDDKIPSELVFSGSYHLTDKIENLGIDAGKLVLSPTRTYAPIVKKMLEK
                     LRPQIHGMVHCTGGGQTKILHFIENLRVIKNNLFPLPPLFQIIQQESQTDWKEMYKVF
                     NMGHRMEIYLPEAYAEEVIDISRSFNVEAKIIGYCEPSDKKELIIESEFGKFEY"
     gene            complement(12819..13037)
                     /locus_tag="PLZ54_04785"
     CDS             complement(12819..13037)
                     /locus_tag="PLZ54_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66279.1"
                     /translation="MNKPTLKSLLKTLLYGLGVFGVYIVIIVLLRLFSGRVPDEAVIF
                     NFFTKNDLLIGLLLACILTFTHQRNKKI"
     gene            complement(13057..15918)
                     /gene="uvrA"
                     /locus_tag="PLZ54_04790"
     CDS             complement(13057..15918)
                     /gene="uvrA"
                     /locus_tag="PLZ54_04790"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOJ66280.1"
                     /translation="MNNTNTDTSEKIEVIGARVNNLKNIDVSIPRDALTVITGLSGSG
                     KSSLAFDTIYAEGQRRYIETFSAYARNFLGNLQRPDVDKITGLSPVISIEQKTVNKNP
                     RSTVGTTTEIYDFLRLLFARAGEAFSYVTGEKMVKYSEDQIVELILKQYEGKRTYLLA
                     PLVRGRKGHYKELFEQIRKKGFLHVRIDGEMKELVPGLRVDRYKNHNIEVVIDRLVVA
                     EKDRRRLKDSMQKAMQQGNGIVMLLEKETDKVRYFSKKLMCPTSGISYDDPDPHHFSF
                     NSPHGACPTCKGLGFVNQVDMDKIIPDKSVSIYNGGIAPLGKYRNTVIFWQIEAILEK
                     YGCQLKTPVKDIPEEAIDEIMNGSEEPLMIKNHSLGITNYSLNYDGILKYIEMQQDDR
                     ASATEQKWANQYSKSVKCPECNGARLRKESLHFLIHEKNIADLAAMDIVELLNWLGNV
                     ENYLTEKQKAIATEILKEIRTRLQFMVDVGLHYLSLGRSSESLSGGESQRIRLATQIG
                     SQLVNVLYILDEPSIGLHQRDNFRLINSLKQLRDSGNSVIVVEHDKSMMLAADYIVDM
                     GPRAGRLGGEVVFAGKPEEMLKAKTLTSAYLNGEKRIEIPARVRKGNGKFLTIIGAKG
                     NNLKNIDVSFPLGVMICITGVSGSGKSSLITETLQPILSQHFYRSLQDPLPYDRMEGL
                     ENIDKVVEVDQSPIGRTPRSNPATYTNVFSDIRNLFATLPEAKIRGYKPGRFSFNTAG
                     GRCETCSGNGYKTIEMNFLPDVYVVCETCNGKRYNRETLEVRFKGKSIADVLDMTINQ
                     AVEFFENQPSILNKIKTLQDVGLGYIKLGQSSTTLSGGESQRVKLATELSKRDTGKTL
                     YILDEPTTGLHFEDIRALLDVLNRLVDKGNTVIIIEHNMDVIKSADYIIDLGPEGGNA
                     GGKVICTGTPAEITRHPESETGKYLKMELDENFMEDLID"
     gene            complement(16089..>16682)
                     /locus_tag="PLZ54_04795"
     CDS             complement(16089..>16682)
                     /locus_tag="PLZ54_04795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOJ66281.1"
                     /translation="SYLPARWWSKATCGLFFIKVKVKGLEKIERGKSYVFAANHQSMF
                     DIFVIYGWLPVRFKWIMKKELRKVPFVGLASEMAGHIFVDRSNAQAAKLSIEKAEKQL
                     KKGNSIVIFPEGTRSTNGQMRKFKKGAFQIAADLALPIVPVTIKGSFERLSKTSFKVK
                     PGVIEMIIHQPIDVTPYLPDRIPELMKTSWEMIHSVL"
BASE COUNT         5045 a         3163 c         3444 g         5030 t
ORIGIN      
        1 aaaaaccgaa caacatgata aaagtaggag ataaagtacg ttttctcaat tctacaggag
       61 ggggaattgt taccagagtt gatgaaaaag aaaaaatcgt gtacgtttcc gatgccgacg
      121 gatttgaaat tcccatgttg gaaagagaat gcgtggttat accagaagta aatgaaaata
      181 ccaattttcc caaaaaagat tttaaatcgg ctccagcagc ttttataagt gagaacgaat
      241 cgccggccgc taaatccatc aacatacaac caaaacctga aattacgaaa gaagaaattt
      301 ttgaaacgcc cgaaggagaa aatctgaaag ttttgctggc ttttgtgcca gaagatatca
      361 agcaacttca gaccacttcc aacgactttt atttggtgaa cgacagcaat tatttcctgt
      421 tttacaacat tgcactgttt gaaaacgaat ggtgtaaaag cgttgcaaac ggcttaatag
      481 aacctaacat gcaggaatgg atagcagaaa ttaaaaaaga agaactgaat gcatgggaaa
      541 aaattcgggt tcaaatcatt ccctttaaaa aagataaaac ttatcggccg caacaagtta
      601 tcgatatcga cctcaaagtg aacctgatga agttttacaa acttcatagt ttcggtgaaa
      661 atgattattt cgatgttcct gccatgatgc tgaatccttt ggaagaaatg gagacccatc
      721 aattgcagaa tatttcaacg gaagaaatta aacaagctat ttcacaaaaa gaaaagcctg
      781 aacggcgacc acgcatcagc aaaccggcaa ataaattgga aattgttgaa gttgatttgc
      841 atatccatca gctggtcgat actacggtcg gcatgaataa taccgatatg ctgaaattgc
      901 agttggaaaa atttgaatcc gttttgaacg aatataaaaa tcggaaagga caaaaaattg
      961 tttttattca cggaaaagga gaaggagtgt tgcgaaatga aattgaaaaa ttgctccgca
     1021 ccaaatacaa aaactattat tttcaggatg cttcctttca acaatatggt tttggcgcca
     1081 caatggttac tattcgataa ccacctgaca caattatttt tgaacgtccg aaatttttga
     1141 atttttaaaa ttattccatt caacccaata atccgggttg gacttttttt tcaaaacctg
     1201 tcaatttttt tattcaatct taaagtatag attatctgaa actataaatt ataattattt
     1261 gaaaatcaga ctcaatactt tgtactctgc agtcaaaact taaaccttta tttcaataca
     1321 aaaacaactt tattaacaaa ataagtatta tggatcaatt atcttttaaa cggtattttc
     1381 tttttgtttt tagtcttatt ttatcagtat cgtttttctc gtgtggcgaa aaggaagaaa
     1441 atgagccgga tattaccaat tctatttatg ttacgcaggt ttttgaatat gtttatgcac
     1501 ccggccaaca tgcccaactg gcaaaacctg ccgatatcga gcattttatt ggtgagcctt
     1561 cggatgaaaa gggatttttg tatttgggcg gttttggcgg atatgtaatt gccgggttcg
     1621 accacgatat tcaaaacaaa gatggatacg atctggaaat atttgctttg cccggagcct
     1681 cgcccgaacc ggcaattgtt tatgtaatgt cagatgacaa cggagatggg caacccaatg
     1741 aaatttggta tgaattaaaa gggaatcagt ttgaaaatga aaaaagaaat tattgggttc
     1801 gctattacaa gccgtcatca tccaatgaca acattcggtg gaaagacagc gaaggagaaa
     1861 gtggggaatt gatttcggga tttggagcgt tgaacacttc ttcatggtgg tggccaacct
     1921 gtatggaaga cagtattact tttgaaggtt cacggttgcc ggatgcttat gacaacaaat
     1981 cttctgaaag tgcacaaagt tgggttgttc caactgatcg ttttatatgg ggttatgccg
     2041 aaaataattt tggaattgat tatgataccc atcatcattc caataaactg gacatttcca
     2101 atgccgtcga ttcttcaggt aaggctgttt atttggaaaa aattcgtttt atcaaaattc
     2161 aaacggccgt ttttcagcgt gccggctggg tgaacgaagt ttcggccgaa attcgcggag
     2221 cagctgagtt gaaataatca gacccttctt ccatacgctt tggaaacctg cataaaaatt
     2281 caaaattact gtcggtcttt ctgttgttac aaaaatttta aatatctttg tgagtttaat
     2341 tttcctcaac gtacaacaat caatttatgg aaaatctcga gccttcagtt gtttggaaat
     2401 ttttcgggga aatcacccaa attccccgac cttcaaaaaa agaagaaaaa ataagggagt
     2461 atttgctgga tgttgcccaa caataccgac ttgaggcaaa aacagaccaa gccgggaatg
     2521 tgctgatttg taaaccggca acattcggtt acgaaaatca caataaaatc attcttcagt
     2581 cgcacatgga tatggtttgt gaaaaagatg gtgatgtgca gcacaatttt gagtccgacc
     2641 ccattcaaac gtttatcgat ggcgattgga taaaggcaaa ggggacaact ttaggagccg
     2701 acaacggcat tggtatggct atgatgcttg ctgtgcttac ttccgacaac atttcacatc
     2761 cggcgcttga gtgccttttt actgttgatg aagaaacagg actgaccgga gcttcggcta
     2821 ttgaagatca tttcctcagc ggacaaattc ttatcaatct ggattcggaa gatgatggtg
     2881 ttatttttat cggttgtgcc ggaggtatcg atacgacaat aaattttcct tttatagctg
     2941 aaaaatctcc gaataattac ttttccttct cgctgaccgt gaaaggattg aaaggtggac
     3001 attccggcga cgacattgac aaaagacacg gaaatgccat aaaaattctg aatcgttttc
     3061 tgtggaatgt aaacaaaaaa cttgatttga gaatccatac ttttcgggga ggaaatttgc
     3121 acaatgccat tccacgcgaa gccgaagtcg tagcttccgt accttttgag gataaggaaa
     3181 aacttcgtgt attactcaac gaatttattg cggaaataga gggtgaactc aaagaagatg
     3241 aaccggctct gcaaatcctt atttcttcag gagaaattcc cgatcaggta ctcgacaaga
     3301 aatcttccga taagctttta aatgcacttt atgcctgtcc acatggagta aaggctttta
     3361 gcagagatat gcccaatctg gtggaaactt ccaccaatct ggcttctgtc cgaatgcagg
     3421 aaaataattc ttttcttatc ataaccagtc agcgcagttc cgttgaatcg gccaagttcg
     3481 acattgccca tcaggttgaa tctgtcttta cacttgctca agctcaagca acgcatggtg
     3541 aaggatatcc gggctggaaa cccaatttga attccgaaat attgcagaaa gccaaagttt
     3601 gttacaaaaa tatcacgtgt gaagaaccga aagttcgggc tattcatgcc ggactggaat
     3661 gcggactttt tctgaaaaaa tatccttatc tcgacatgat ttccattgga ccgcaaatgt
     3721 atggagttca ttctcccaat gagcggttaa gtatctcttc tacccggaaa tgttggcaat
     3781 ggttgcttaa aattctcgaa aaaagctgaa atgacaaata tctcgggaaa caactcgctc
     3841 ggagaaagtc ggatacaacc caacaaactc aaaattatca atgatccggt ttttggtttt
     3901 atcaacatac cggatgattt tttatatgaa attattcagc atccttattt tcaaagatta
     3961 aaccgcatca agcaattggg tgtttcgttc tatgtttatc cgggagcgca acatacccgt
     4021 ttgcaacatt cgttgggggc catgcactta atgggtgaag ccattgaaga gttgcgtcag
     4081 aaaggtcatg tgatttccag cgaagaagag caggctgccc gggcgtgcat gttgctgcac
     4141 gacattggac acggcccgtt ttctcatgct ttggaaaaca gcttggtaaa aggaattcac
     4201 catgaagaaa tttcaataat gctcatgcag caattgaata aagaaatgaa taataatttg
     4261 gctttggcgc tggaaatatt tagtggccat tatcctaaaa aatttcttca tcagttggtt
     4321 tcgggacaac tcgacatgga tcgaatggat tacttgagca gagacagctt tttttgcgga
     4381 gtcagtgaag gtatcattgg ttcggcgcgt attatcaaaa tgttgaatgt ttttcacgga
     4441 gaattggtcg ttgagttgaa aggaatttat tccattgaaa aatttctggt ggcacgtcgt
     4501 ttgatgtact ggcaggtgta tttgcacaaa acgctggttg ctaccgaaaa aatgttgaca
     4561 aatattctca agcgtgccaa ggaacttgct tccgttggtg aaacattatt tgcttctcct
     4621 gctttgcatt attttctcta tcatcatctg gacaagcaga attttgttcg ttcaaccgaa
     4681 gcattggatt attttgttca gcttgatgat tcggatgtga tttgtgccat caaagtttgg
     4741 gctcaccatc ccgataaagt tctttctttt ctttgcaatg cttttatcaa cagaaaattg
     4801 tttaaggtag aaatgctgca ggagcctttg gacgaaacaa aaaaagagga attattgaaa
     4861 aaatacagcc ggtattttga tgtttcgcag caggaagccg aatatttcat gggagaggaa
     4921 atcgtcagca cagatacgta tgaccctgtt gatgagaaaa ttaacattct atacaaagat
     4981 ggaacggtga aagatattgc cgacgcgtcc gatatgctta atataaatgt tttgaccaag
     5041 aaagtttaca agaattattt ctgttattgc cgaatttagc cgacagcaag atatttttct
     5101 gtaaaataaa attgtagaat aaaaataaca agtgttacaa agtgaataat taaaaattct
     5161 ttcgtacttt tgtacccaaa atttttaaaa actatggagt ttacagctca gcaaattgca
     5221 gattttttag gtggccaggt ggaaggagac ccatcagtaa aggtaagtaa tttttctaaa
     5281 atagaggaag gaaaaccagg aaccataaca tttttgtcca atccaaaata cagtcattat
     5341 atttacgatt gtaatgcaag cattgtattg gtaaacaagg atttcaagcc tgagaaagaa
     5401 attccagcga cgcttattcg tgtcaacaat gcgtatgaat gtttggcgca gcttttttcg
     5461 ctggtcgaac agagcaaacc caaaaaaaag ggcatttctc cattggcttt catagcttct
     5521 actgcccaaa ttggtgaaga tgtttacatt gctccctttg tttatgtggg tgaaaatgta
     5581 aaaataggaa aaaacacgca aattcattct catagcaata tcgaagacaa ttcttcggta
     5641 ggcgaaaata ccatattgtt ttcaggtgta catgtttatc ccgattgtag cattggcaac
     5701 aattgcattc tgcattccgg tgtggtaatt ggtgccgatg gttttggttt tgcccagtcg
     5761 gaagatggta cttacaaaaa aattacccaa atagggaatg tcgtgttgga agacgatata
     5821 gaagttggag ccaacaccac cattgatcgc gctacgttgg gaagcaccat tgttcacaaa
     5881 ggtgtgaaaa ttgataatct ggtgcaaata gcccataacg acgaaattgg tgaaaacaca
     5941 gtaattgtag cgcaaacagg cattgccggt tcaaccaaaa tagggaaaaa ttgtgtgata
     6001 ggcggacagg tcggtatagt tggacatttg cagattgctg acaataccca aatcggggca
     6061 cagtcgggtg ttgccaattc ggtaaaaact cccggtcaga ttattatcgg ttctcctgct
     6121 attcccattc agaattttcg ccgttcttcc attgtattta aaaatctctc cgatttacaa
     6181 aaaatggtga tagaattaca aaataaggta gaagagctgg aaaaaactaa aacttcagaa
     6241 aaataatttt ttctgaaatt ttttttgact gcctcaaatg cataaaaatt gttaatcaaa
     6301 aacaaatgac gagacaaaga acattaaagg aatcttttac attagaagga aaaggcttac
     6361 acacaggttt ggatattgtg attacctttt tgccggctcc agaaaatcat ggaataaagt
     6421 taaaacgtat tgatctggaa gaacccgtta tcattgaagc attggcagaa aatgttaccc
     6481 acacggtacg tggaactgtt ctacaaaaag gcgacgttca agtaagcacg gtagaacatg
     6541 cgttggctgc actttatgct tttggtattg acaattgcct gatagaagtc aatgcacctg
     6601 aattccccat tttggatgga agtgcccgct tttatgcaga aaaaattaaa gagatcggaa
     6661 ttattgaaca gaaagcggaa aaagattttt atgtggtaag gaaaaaaatt gaatattcac
     6721 ttcccgattc caactccaaa atcactttgt tgcccgatga tgttttcagt gtagatgttc
     6781 acataggatt cgattctgcc gtacttgcca atcagtatgc aagtttggat tcactcgata
     6841 aatttgccga tgaaattgct tctgctcgta cttttgtgtt tgttcacgaa attgaaccgc
     6901 tcttgagcat gaatctcatc aaaggtggcg atttgaaaaa tgcacttgtc atttacgata
     6961 aggaattgtc gcaggatacg atggacagac tcaccgacaa acttcatcag ccacgtattg
     7021 atgcaaccaa acttggatat atcaacggtg aactgaatta tcccaatgag cctgctcgac
     7081 ataaattact ggatgttatc ggagaccttt ccctgattgg gaaaccactg aaagcaaaag
     7141 ttattgtgca gtatcccggt cataaaataa ataccaatct ggccaaaatc attcgcaagg
     7201 aaataaaaaa gcaggaagtt tcggctcctg tgtacgacga ttcggttccg cccgtattgg
     7261 acattaatgc tattagaaaa cttttgccgc atcgctggcc ttttctgctg gtcgataaaa
     7321 ttatcgatat caacgaaaga gtgattgtgg gtgtgaaaaa tgtatccggc aacgaaacgt
     7381 tttttatggg acattttccc aacgaacctg ttatgcccgg agttcttctg gtagaagcta
     7441 tggcgcagac tgccggaatt ttggtgttga acaaagtgga ggaacccgaa aaatattcta
     7501 cttattttat gaaaatagac aatgtaaaat tccgtcagaa agttgtaccg ggcgatacag
     7561 ttatttttcg attagaaatg atggatgaaa ttcgtcatgg atgcaccgat atgaaaggtt
     7621 acgcttttgt aggcggaaaa attgttgctg aagcccagtt tatggcacaa atcatcaaaa
     7681 acaaataaaa ctaaaagatt tacacccata caatgaaaca accgctggca tatattcatc
     7741 cggatgcaaa aattgcaccg tcggtagtca ttgaaccatt tgttaccata gataaaaatg
     7801 tggagatagg cgaaggaacg cgtatcggtc cgaatgttac cattttggaa ggagcccgaa
     7861 tcgggaaaaa ctgtactatt tttcccggtg ccgtaattgc agctattcca caggatttga
     7921 aattcaaagg tgaagatact ttggccatta ttggcgataa taccaccatt cgcgaatgtg
     7981 ttaccgtaaa cagaggcaca gcttcaaaag gaaaaaccca aattggcgac aattgcctga
     8041 tcatggctta ttgccacatt gcacatgatt gctttgttgg cagcaatgtc attatgtcca
     8101 atgccacgca gcttgccggc gaagtattca ttgatgatta cgcggtgatc agtggaggcg
     8161 tcttggtgca tcaatttacc catattgggt cgcatgtaat tattcagggt ggaacaaaag
     8221 tgaataagga tgtaccacct tacgtgaaag cagggcgtga gccgctgtca tttgaaggaa
     8281 tcaattcggt gggtttgcgg cgacgcggat ttaccaatga acaaattggt tttattcagg
     8341 aaatttatcg ctatatcttt ttgtcgaaaa tgaatgtgag caatgccata gatcgtatcg
     8401 aagccgaatt gcccgctacc aaagaacgtg atgacattct tcttttcata aaaaattcgc
     8461 aaagagggat tatcaaaagt tttctggaat aaacttgatt tcatttctct gtttttgaga
     8521 aaaaattttt tatatcattc agaagctatg aaaattaacg aagaagaaac tccaaaaaaa
     8581 aatctgaatt tcattgaagc tattgttgat caggacgtta aggaagggaa aaatggcgga
     8641 cgtgttcaga caaggtttcc tcccgaaccg aacggttatt tgcatatcgg acatgctaag
     8701 gctatttgtc tgaatttcga tattgcaaaa agatacggag gaatttgtaa tcttcgtttt
     8761 gatgatacca atccggtaaa agaagatgtt gaatacgttg attccataat ggaagacatt
     8821 caatggttgg gttatcagtg gaaaaatgtt tactacgctt ccgattattt tcagaaactt
     8881 tgggatttag cagttctgtt tatcaaaaag gggttggctt atgtagacga acaaagtgcc
     8941 gaaaccatag ccaaacaaaa aggtacacct actgttcccg gaatagaaag tccttatcgg
     9001 aatcgtccga tagaagaaaa tcttgcgctt tttcaaaaaa tgacgaatgg tgaaattccc
     9061 gaaggcggca tggtgttgag ggcaaaaatc gatatggctt ctcccaatat gcactttcgc
     9121 gatcccatca tgtacaggat tattacttcc catccgcatc accgtacggg ttggcaatgg
     9181 aaagtttatc cgatgtatga ttttgctcat ggtcagtgcg attattttga aggagttact
     9241 cattctttgt gtacgcttga gtttgaagtt catcgaccac tttacgactg gtttatcgat
     9301 cagtatcagg ataccgatta tcgaccgcga caaatagaat tcaatcgtct caatcttact
     9361 tataccgtca tgagcaagcg taaacttttg caacttgttc aggaaaagtt tgtcagtggc
     9421 tgggacgatc cccgcatgcc aacactttgt ggtttgcgtc gacgtggttt tacacctgaa
     9481 tccatacata actttatcga taaaataggc tatacaaaat ttgaagcgct gaacgatatt
     9541 ggattgcttg aacatgctgc ccgcgaaagt ctcaacaaaa cggcaatgcg tgttaatgcc
     9601 gtacttcatc ccgtcaagct gattgttacc aattatcctg aaggaaaagt agaaatgatg
     9661 acaacgcaaa ataatcctga agaccctgaa gccggaacca gagaagttgt cttttcgaga
     9721 gagcttttta ttgaaagaga cgattttatg gaaaatcctc caaaaaatta ttttcgcctg
     9781 atgcccggtc aggaagttcg gctgaaaagt gcttatattg ttaaatgtac gggttgcaaa
     9841 aaagatgaaa atggtgttgt taccgaagtt tattgcgaat atgaccccat gacaaaaagc
     9901 ggcatgcctg aaagcaatcg caaggtgaag ggaacgcttc actgggtttc ggctgctcat
     9961 tgtgtggaag ctgaagtacg cctctacgac cgcttattta acgttgaaaa cccttctgcc
    10021 gacgaacgcg attttcgcga attactcaat cccgattcct tacaaatact taccggttgc
    10081 aaactggaaa attcacttaa agatgccaag cctctcgaac atttccagtt tcaacgcatt
    10141 ggatatttta cagtcgattt ggactcaact ccggacaagt tgatattcaa tcgcactgtc
    10201 tctctcaagg acagttggag taaagaactc aataagtaaa atcacctcaa atacttgcca
    10261 agaatgttgt aatgaatatt tgcagcattg ttgacttttg tagtggtctg gattggaatt
    10321 caggaatttt ttatgcaata aatttctaaa ttgagaattc tgtgttatat tcgcagatga
    10381 attgcgaata aagagaacga acttgagtga aaaagttcat taacttatag tgctgtattt
    10441 cggatctata aatgataaag aaagcatatt caatagcgca atgaatcgat tccaattaaa
    10501 tttcttaaag aatgttggag ctcatccgag tcgattcgaa aatgtgcaca aaaaaatttg
    10561 gaagaatggc gtgaatatta tttcgataat ctcgggtcaa aagaatatat tattgaactt
    10621 gggaaaacgc ttttatttag ataactgaag tgatccatgc agaagttgcc gaaataaccg
    10681 aacaggattc catcgattac atgatacaac aggtaattga cagaatttat gatggttatg
    10741 taacccaaag ttaaactatt tatggacaac ttcaaaatat actacctgta aaagtagaac
    10801 ttgcacccga tgagcgggac agaagattta acgtggactt tttattcaaa taggggaaaa
    10861 attcattcgg ctttaaattg aaactgtttc attaagaaat agaactcaac aaacaattaa
    10921 aactatggac ttcagtttaa tacaaattgc cagtgccttt atggtactgt ttgccattat
    10981 tgatattttg ggctcggtgc ctattatttt ggctatcaaa agtaaaggtg aaaatgttca
    11041 tgccggaaaa accagcttgg tggctttggt cattctggtg gtttttttat ttgccggtga
    11101 ctggattctt caacttttca atgtcgatat tgaatctttt gctgttgcgg gttctttcgt
    11161 gatttttatt ttttcgctgg aaatgattct cgatgtggaa attttcagga acaaagggcc
    11221 ggaaggagct tcttcggtag tgccgatcgc ttttccattg atagcggggc caggttcgtt
    11281 taccacatta ctttccttac gcgcggaata tgccatcgaa aacattataa tagcgctcgt
    11341 gctgaacatt tgttttgttt tcttcgtgtt acggttcaca tccaaaatag aaaaatggat
    11401 ggggaaagga ggaatttata ttttaaggaa atttttcggc attattttgc tcgccgtttc
    11461 agtaaaacta tttactacca acattagatt tttgctgtag ttggcgcgtt ttttcggcat
    11521 tttttggaat aaaatatatt ggcagaaatt atcttgctgc tatcaatatt caaatttccc
    11581 gaattcgctt tctatgatta attctttttt gtcggatggt tcgcaatacc cgataatttt
    11641 tgcttcgaca ttgaatgagc gggaaatgtc aatgacttct tctgcataag cttcgggcag
    11701 ataaatttcc atgcgatgtc ccatattgaa aaccttatac atttctttcc aatcggtttg
    11761 ggattcttgc tgtatgattt gaaaaagcgg cggcaagggg aaaaggttat ttttaattac
    11821 cctgagattt tcaataaaat gtaaaatttt tgtctgacct cctcccgtac aatgaaccat
    11881 tccatgaatt tgcggacgaa gtttttccag catttttttt acaatcggag catacgttcg
    11941 ggtaggtgaa agcaccagtt tcccggcatc gataccgagg ttttcaattt tgtcggttaa
    12001 atggtaagaa cctgaaaaaa ccagttcgga aggaattttg tcatcgtagc attcgggata
    12061 tttttcggcc aaataatgtg caaaaacgtc atgtcgggcg gatgtcaaac cattgctgcc
    12121 catgccgccg ttgtactcct tttcgtaact ggcctgacca cttgaggaaa gccccacaat
    12181 tacatcgccg ggacgaatat tggcattgtc tattacatcg gctcttttca ttcggcaagt
    12241 tacagtgctg tccacaataa tggttcgaac caagtcgccc acatcggcag tttctcctcc
    12301 tgtgaaatac agttttacgc ccatttcgcc aagttgttga atcagttcgt ccgttccgtt
    12361 gatgatagcc gaaacaactt cgccgggaat caggtttttg tttcttccaa tggtggagga
    12421 aaccaaaatg ttatccactg ctcctataca caacaaatcg tcggtattca tgattagcgc
    12481 atcctgagcg atacctttcc ataccgacaa atcgcctgtt tctttccagt aggcataagc
    12541 caacgaagat tttgtgcctg ctccgtcagc atgcatgatg ttgcaataat cgggattgtt
    12601 tcccagaata tcgggaatga ttttgcaaaa agcctttgga aaaattccct tatccaaatt
    12661 ccggatcgca ttgtggacat cttcttttga tgctgaaact ccgcgtaaat cgtaacgttg
    12721 gtttttcata acagttttaa atggattttt aatattttat ttgcaaatca tcggtttaat
    12781 tttcgcaaga aataagaact tcaactcatg tcaaaaaatt atattttctt gtttctttga
    12841 tgggtgaaag tcaggataca ggccaataaa agaccaatca acaaatcgtt tttggtaaaa
    12901 aagttgaata tcacagcttc atccggaacg cgaccactga aaagtcggag taaaacaata
    12961 attacaatat atactccaaa tactccaagt ccgtaaagaa gcgttttcaa cagcgatttc
    13021 agtgttggtt tgttcataat ttatgagttt aatttttcaa tcgatcaaat cttccataaa
    13081 attttcgtcc agttccattt taagatattt ccccgtttcg ctttcgggat ggcgagttat
    13141 ttcggccgga gttccggtgc aaataacttt tccgccggca tttccgcctt cagggcccaa
    13201 gtcgatgata taatcggcac ttttgataac gtccatgttg tgttcgatta taatcaccgt
    13261 atttccttta tcaaccagcc gattcaaaac atcgagcaaa gcgcgtatat cttcaaagtg
    13321 caagccggtg gtcggttcgt ccaaaatata aagtgttttt cccgtatcgc gtttggacag
    13381 ctcggttgcc agcttcacgc gttgactttc tcctcctgaa agggtggtgc tggattgtcc
    13441 gagtttaata tagccaagtc ctacatcctg aagtgttttt attttattga gaatggatgg
    13501 ctgattttca aaaaattcta ccgcctgatt aatggtcatg tccaacacat cggcaatgga
    13561 ttttccctta aatcggactt ccagtgtttc tctgttgtag cgtttgccgt tacaggtttc
    13621 acaaaccacg taaacatcgg gaagaaaatt catttcaata gttttgtaac cgtttccgga
    13681 gcatgtttcg caacgaccgc cggcagtgtt gaatgaaaat cgaccgggtt tgtagccgcg
    13741 aattttagct tcaggcaaag ttgcaaataa atttcgaata tccgaaaaaa cgtttgtata
    13801 ggttgccgga ttggatctgg gcgtgcgtcc aataggagat tgatcaactt ccacgacttt
    13861 gtcgatattt tccaatcctt ccatccggtc gtaaggaagc ggatcctgta acgaacggta
    13921 aaaatgctgg ctcaatatgg gttgaagggt ttccgtgatt aatgaagatt ttccgctacc
    13981 tgaaactccg gtaatacaaa tcatgacgcc caaagggaac gacacatcga tatttttcaa
    14041 gttatttcct ttggccccga taatggtcag aaatttcccg tttcctttgc gcacacgagc
    14101 aggaatttct atacgttttt ctccattcag ataggcagaa gtcaatgttt tggctttcaa
    14161 catttcttcc ggtttaccgg caaaaactac ttctccaccc aagcgaccgg ctcgtggccc
    14221 catatcgact atataatcgg cagccaacat catacttttg tcgtgttcca ctacaataac
    14281 cgaatttccg gaatcgcgca gttgtttcaa tgaattgatc agccgaaaat tatcgcgttg
    14341 atgcagtccg atgctgggtt cgtccagaat atacaacaca tttaccagtt gtgaaccaat
    14401 ttgcgttgca agcctgattc gctgactttc tccacctgaa agcgattcgg agcttcgacc
    14461 cagagaaaga tagtgtaacc ctacatcgac catgaactgc aaacgggtac gaatttcctt
    14521 taaaatttcg gtggctatgg ctttttgttt ttcggtaaga taattttcaa cgttccctaa
    14581 ccaattcaac aattcgacaa tatccattgc ggcaaggtcg gcaatgttct tctcgtgaat
    14641 aaggaagtgg agggattctt ttcgcagtct ggctccattg cattccggac atttcaccga
    14701 tttggaatat tgatttgccc atttctgttc ggtggcagaa gctctgtcat cctgctgcat
    14761 ttcgatatat tttaaaatac cgtcataatt caaggaataa ttggtgatac ccagactgtg
    14821 gtttttaatc attaaaggtt cttccgagcc attcatgatt tcgtcgatag cctcttcggg
    14881 aatatctttg acaggagttt tcagttggca gccgtatttc tccaaaattg cttctatttg
    14941 ccaaaaaatc acggtatttc tgtattttcc caatggagct ataccgccgt tgtaaatgga
    15001 aaccgattta tcgggaataa ttttgtccat gtcaacctga ttgacaaatc ccaatccttt
    15061 gcaagtggga caggcaccgt gaggcgaatt gaaagagaaa tgatgcggat cggggtcatc
    15121 gtaagaaatt ccgctggttg gacacattaa ttttttgctg aaataacgta ccttgtcggt
    15181 ctctttttcc aaaagcatca caattccatt gccttgctgc atggcttttt gcatggaatc
    15241 cttgagtcgg cgacgatctt tttcggcgac caccagtcgg tcgataacaa cttctatatt
    15301 gtggtttttg tatcgatcaa cgcgaagtcc gggaaccaat tccttcattt ctccgtcgat
    15361 acgtacatga agaaatcctt tttttcggat ttgttcaaac aattccttat aatgtccctt
    15421 gcgcccacgc accaatggtg ccagcaagta agtgcgcttg ccttcgtact gtttcaaaat
    15481 cagttctaca atctgatctt ccgaatattt taccattttt tctcccgtaa cataggaaaa
    15541 agcttcaccg gcacgggcaa aaagtagtcg gagaaagtcg taaatttcgg tagtggtgcc
    15601 aacggtagaa cgtggatttt tgttgaccgt tttttgctca atcgaaatga caggacttag
    15661 acctgtaatt ttatcaacat caggacgttg cagattacct aaaaaatttc tggcatacgc
    15721 cgaaaaagtt tcgatatagc gtcgctgtcc ttcggcataa atggtatcga aagcgaggga
    15781 tgattttccg cttccactca atccggtgat gacggtgagg gcatcgcggg gaatggaaac
    15841 atcgatattt tttaagttgt tgacacgtgc tccaatgact tcaatttttt ctgaagtatc
    15901 tgtattcgtg ttatttatat tgttgttgtt tgacaaagtc agcctccttt aaattgaacc
    15961 tgcaaaggta caaaaaatga aggtagtttc aaatattgga ataactcaaa gcaatgcaaa
    16021 aattgaatat tttgtttttt cgaaaaatat tcttctcaat aagccaagag taaaaaattt
    16081 taataaaatt ataaaacaga atgaatcatt tcccaagaag ttttcatcag ttcgggaatg
    16141 cggtcgggca gataaggtgt tacatcaatt ggctgatgaa taatcatttc tataacgcct
    16201 ggtttgactt tgaaactcgt ttttgataat ctttcaaagc ttcctttgat ggtaaccgga
    16261 acaataggga gtgccaggtc ggcagcaatt tggaaagcgc cttttttgaa ttttctcatt
    16321 tgtccgtttg ttgaacgcgt cccttcagga aaaataacga tggagtttcc ttttttcaat
    16381 tgtttttcgg ctttttcgat gctgagtttt gcggcttgtg cattcgaacg gtctacgaaa
    16441 atatgtccgg ccatttcgct ggccaatcct acaaaaggaa cttttctcag ttcttttttc
    16501 atgatccatt tgaaccgtac gggaagccag ccataaatta caaaaatatc gaacatgctt
    16561 tgatgattgg cagcaaaaac ataggatttt cctctttcaa ttttttccag tcccttgact
    16621 ttcactttga tgaagaataa tccgcaggtt gctttcgacc accatcgtgc cggcaaataa
    16681 ga
//
LOCUS       DAOSCH010000043        16527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2407_length_16527_cov_6.742533, whole genome shotgun sequence.
ACCESSION   DAOSCH010000043 DAOSCH010000000
VERSION     DAOSCH010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16527
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2407_length_16527_cov_6.742533"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /gene="trmD"
                     /locus_tag="PLZ54_04800"
     CDS             complement(<1..666)
                     /gene="trmD"
                     /locus_tag="PLZ54_04800"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOJ66282.1"
                     /translation="MRIDIISAVPQLLESPLNHSIVKRAQEKHLVEIFVHNLHDYSYD
                     KRRNVDDYAFGFSAGMVLRIEPIDRVISKLKQERNYDEIIYTSPDGEMFNQKIANQLS
                     LCENIIILCGHYKGVDQRVRDHLITREISVGDYVLTGGELPAAIMTDAIVRLLPGAIG
                     DETSALLDSFQDNLLAPPVYTRPADYKGWKVPDVLLSGHEANIRQWLHAQSVERTRRR
                     RPDL"
     gene            complement(710..1624)
                     /locus_tag="PLZ54_04805"
     CDS             complement(710..1624)
                     /locus_tag="PLZ54_04805"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOJ66283.1"
                     /translation="MPKATFTILGCGSALPTKKNFPSSFLLDLREKQFLIDCGEGAQI
                     RLRQMGLKTNRLGHIFISHLHGDHCLGLLGLISSFSMLHRTADLHIHAHPDLEKIMDV
                     PLHYFSAETPFQLFFHPFNPQVNELIYEDRTVKVSTIPLKHKVPTCGFLFEEKPLERH
                     LIREMIDFYRIPVSQLHKIKQGEDFVTEDGEVVPNKRLTYENVSPKRFAYCSDTAYDE
                     SIVPIIRQVDCLYHEATFLEEDIVRADKTLHSTALQAAEIAKKAEVGKLIIGHYSARY
                     ADQSVLLNEAASVFQQVIEGQDMGVYEI"
     gene            complement(1691..2443)
                     /gene="lptB"
                     /locus_tag="PLZ54_04810"
     CDS             complement(1691..2443)
                     /gene="lptB"
                     /locus_tag="PLZ54_04810"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOJ66284.1"
                     /translation="MSNDEMVLRTEHLVKRYGKRTVVDDVSIEVRQGEIVGLLGPNGA
                     GKTTTFYMVVGLVTPNAGKIFLNDIDITKYPVYKRAQSGIGYLAQEPSVFRKMTVEDN
                     IKSVLEMTKFTKEYQKKKLETLIEEFNLEHVRKNIGDRLSGGERRRTEIARCLAIEPR
                     FILLDEPFAGVDPIAVQDIQDIVWKLKDKNIGILITDHNVDETLAITDRAYMLFDGRI
                     MFQGSALELSENPIVREKYLGRDFELKKKTKV"
     gene            2631..3086
                     /locus_tag="PLZ54_04815"
     CDS             2631..3086
                     /locus_tag="PLZ54_04815"
                     /inference="COORDINATES: protein motif:HMM:NF025698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HOJ66285.1"
                     /translation="MKVEIFCPITNKITDENIARLNAGFTVVLLLSYFVTSNIVPVVF
                     LLLDFTARAIERPEWSILSKTSKWILKKLHVESKLINAGPKIFAARIGSFFSLITILF
                     FWLKLPSISIALAAVFFVFALLESAFNYCVACRIYPFIYHALQAKDKEK"
     gene            complement(3114..4610)
                     /locus_tag="PLZ54_04820"
     CDS             complement(3114..4610)
                     /locus_tag="PLZ54_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOJ66286.1"
                     /translation="MELAVEFYFLVASVLLILSIVAGKTGYRFGVPALLLFLLIGILA
                     GSDGLGIEFNSPKQAQLVGVVALNIILFSGGMDTRFSEIKPVLWQGIILSTFGVLLTA
                     FITGSFIYWLTNHLFTAITFSFLESMLLGSIMSSTDSASVFSILRSKKLSIKHNLRPL
                     LELESGSNDPIAYLLMIIFIQLLQSSDISSGEVVAFFFRQFIIGAICGYILSKMVIFI
                     INRIRLDYEGLYSVLLLSFLFFLYSFTSILGGNGYLAVYIGGLFIGNQRFVHKKSSLK
                     FFDGLTWLFQIIMFLTLGLLVNPHELLPIAGAGILIGLFMIVISRPISVFILLSPFRK
                     INFKSKLFVSWVGLRGAVPIIFATYPLMSGIPQARMMFNIVFFITILSLVLQGSTIPF
                     VARLLKLSMPSKDKPKLKEFDIDLPEDIKSAISEITVTEEMLQKGRKLMDITLPDQTL
                     AVMIKRNDHFFVPRGNTKLEIGDTVLLIADNDEIMKETFKKLGVDKEF"
     gene            complement(4926..6464)
                     /locus_tag="PLZ54_04825"
     CDS             complement(4926..6464)
                     /locus_tag="PLZ54_04825"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007755582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOJ66287.1"
                     /translation="MSQEDVFKKLIAHCKEYGFVFPSSEIYDGLSAVYDYGQNGVELK
                     NNIKKYWWDSMVLLHENVVGIDAAIFMHPTTWKASGHVDAFNDPLIDNKDSKKRYRAD
                     VLIEELIAKYDEKIEKEVEKAAKRFGENFDENLFRATNERVKEHQAKRDEIHRRYVKA
                     LNDNDLAELRAIIIDNEIVCPVSGTKNWTEVRQFNLMFTTEMGSNADSAMKIYLRPET
                     AQGIFVNFLNVQKTGRMKIPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQFFVRPG
                     EEMKWFEYWKAYRMKWHKALGLGDHKYRFHDHDKLAHYANAATDIEFELPFGFKEIEG
                     IHSRTDFDLKNHEKYSGKNLKYYDAELKESYTPYVIETSIGVDRTFLSIMASAYTEEK
                     LEDGETRVVLKLPPVLAPVKIGIFPLIKKDGLPEVAHKIFEDLKFDFKCFYDDKDSIG
                     KRYRRQDAIGTPFCITVDHQTLEDETVTIRYRDSMKQERVKIEALSAILNEKVSMKNL
                     LKQL"
     gene            6773..>7074
                     /locus_tag="PLZ54_04830"
     CDS             6773..>7074
                     /locus_tag="PLZ54_04830"
                     /inference="COORDINATES: protein motif:HMM:NF023205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3316 domain-containing protein"
                     /protein_id="HOJ66288.1"
                     /translation="MLVAWMMIAAFSLNAQDIILQTRTLTTSTHAFGFSTINMLDSYL
                     SPLKYSGSGFRYENDNRKFVSPENIRLSKENHFNMNFGLTENPAQTSEMWYLGMNFS"
     assembly_gap    7075..7174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7175..7767
                     /locus_tag="PLZ54_04835"
     CDS             <7175..7767
                     /locus_tag="PLZ54_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66289.1"
                     /translation="TENPAQTSEMWYLGMNFSWGMHRHFYVGENLLLLAGGLWDLDFM
                     GKTNSRNQNNPFNIDLATNLNLSGEAVYRIRTPKRMLRLGFSMQAPLVGVMFVPEQGA
                     SYYEIFELQNFSNTLHFTSLHNKLSLTENLSLDIPFNHTTLRFGFRNENLKFEANQMI
                     FKRNDLSFHVGFIYDFYKFEGTKNRAPSSFNSTYNY"
     gene            7783..8793
                     /locus_tag="PLZ54_04840"
     CDS             7783..8793
                     /locus_tag="PLZ54_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ66290.1"
                     /translation="MKKHIVLLSLFGMLFHSCMDEPVIQHNTYEGNFNALWEIIDTKY
                     CYLDYKNINWDSVYTVYHQRIPYIKTDLDFFDLLGKMLEELKDGHVNLYSGFDVSRYW
                     KWYTDYPENFSSDIIYSNRYLGENYRIAGGMHYQKIDNGNIGYIYYGDFTSDFSDTNM
                     GYIFKYFSNCKGIILDVRNNGGGYMSGAEQLASYFFTEKTLTGYLCHKTGPGHTDFST
                     PIKLFTPANKNVQWLRPVVILTNRMTYSATNDFVNRMRKAPYAIILGDKTGGGGGMPL
                     SSEIPNGWMVRVPTSPMFDANMQHIEWGIDPDINIALKPEDAARGYDTLIERAVALIK
                     FL"
     gene            8845..9384
                     /locus_tag="PLZ54_04845"
     CDS             8845..9384
                     /locus_tag="PLZ54_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOJ66291.1"
                     /translation="MQKLKVTEMNRLTVEEFKKKEKTPLIVVLDNVRSLHNVGSIFRT
                     ADAFLVQAVYLCGITSTPPNAELHKTALGAENSVEWKYFEETHKALEELRMMGYTIFA
                     IEQARESLPLNSIELNPANKYAIVLGNEVKGVQQTVVDACDGCIEILQYGTKHSLNVS
                     IAGGIVVWEFFKKLMEERN"
     gene            complement(10017..11771)
                     /gene="sppA"
                     /locus_tag="PLZ54_04850"
     CDS             complement(10017..11771)
                     /gene="sppA"
                     /locus_tag="PLZ54_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOJ66292.1"
                     /translation="MKQFFKYTLATILGVILAGIILVAILFGIAGGIAGQKSAPTKLK
                     PNSVYELKLNGVLVERSEEDPFSIALAQAMGNEDNLTIGLNDILSNIQKAKTDENIAG
                     IYLNFGTLSAGYASMKEIRDALKDFKKSGKFVVAYADNYLQHEYYLASVADKIFLNPQ
                     GMLDWKGLASEITFYKNTLDKLGIEMQVVKVGTFKSAVEPYVNTKMSDANREQVTVYL
                     NSIWNTLLSEVSDSRKIPFEKLNVYADEMLTFQPTEKAKQYALVDSLVYQDEIDFIVK
                     GFLKDSTANINYVGHEAMCKVPDATKYDRNKIAVLYAAGAIDNGSDGIQSKKLVKTIN
                     EIAKDNSVKAVVFRVNSPGGSAYGSEQIWHALSQLKQKKPLIVSMGDYAASGGYYISC
                     IADSIVAQPNTITGSIGIFGLIPNIEGLDKKIGLTFDGVKTNKMSDAISINRPFRPEE
                     RDLMQNYVNRGYELFVKRCADGRKMSVEQIKAIAEGRVWTGKDAMEIGLVDKLGTIND
                     AISMAVKKAKLTSYQIKEFPKIETFSEKLLKELSDGTETRIEKLKLGEHYQMFKMIEQ
                     LDKMDGLQARLPYELTIR"
     gene            complement(12081..13580)
                     /locus_tag="PLZ54_04855"
     CDS             complement(12081..13580)
                     /locus_tag="PLZ54_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HOJ66293.1"
                     /translation="MQNKFKFVSAEEAATYVQHGDVVGFSGFTPAGCPKAVPVAIARR
                     AETFHAEGKEFKIGMYTGASTGVSVDGALAKADAVLFRTPYQSNKDMRNAINAGKAPY
                     FDLHLSTMAQELRFGFLPKPKFAILEVCDWNENGELVLTAAVGISPTIAEMADHIILE
                     WNHYHPKELKGFHDIYQPQDPPYRREIPIYKPSDRIGSEILKVDPAKILCIVETNLPD
                     EVSDFAPLDEVTQQIGNNVATFLAGEIRAGRLPHDFLPIQSGVGNIANALLFSLGKNE
                     EIPPFEMYTEVIQDAVIELMLEGNIKFASGCSLTVTDAVLQKIYQNIDFFRDKLVLRP
                     QEISNNPEMVRRLGLITINTALEADIFGNVNSTHVLGTKMMNGIGGSADFTRNSFISI
                     FTTPSTAKNGNISSIVPMVSHVDHTEHSVNVLITEYGIADLRGTTPKERAEIIIKNCV
                     APEYKQMLRDYLAISGSSHTPNNLKACFGMHIQLEKTGSMLGTNWDDFK"
     gene            complement(14102..15025)
                     /locus_tag="PLZ54_04860"
     CDS             complement(14102..15025)
                     /locus_tag="PLZ54_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ66294.1"
                     /translation="MKILVATDKPFAKVAVDGIRAEVEAAGYELALLEKYTEKQQLLD
                     AVADADALIVRSDIVDAEVIKAAKKLKIVVRAGAGYDNIDLDAATQANICVMNTPGQN
                     SNAVAELVFGLLIYAVRNFYNGSSGTELKGKKLGIHAYGNVGRNVARIAKGFDMQIFA
                     YDAYCPAEVIKNDGVTPLTSVEELYASCDMVSIHIPATPETKKSINASLLQKLPKGAV
                     IVNTARKEVINEEDLINCMELRNDIKYVTDIMPDQHAVFAEKFPVRYFSTPKKMGAQT
                     AEANINAGIAAAKQIVSFFKTGDERFRVNKK"
     gene            complement(15114..16181)
                     /gene="serC"
                     /locus_tag="PLZ54_04865"
     CDS             complement(15114..16181)
                     /gene="serC"
                     /locus_tag="PLZ54_04865"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOJ66295.1"
                     /translation="MKKHNFNAGPSILPREVIEKTAQAVLDFNGSGLSILEISHRSKD
                     FQAVMDEAIALFKELLEIPSGYSVVFLGGGASLQFCMVPFNLLEKKAAYLLTGTWASK
                     AYKEAKGFGEVIEVASSKDANFSYIPKNYVIPSDVDYFHITTNNTIFGTEILTDIDSP
                     VPLVADMSSDIFSRPVDVSKYGVIYGGAQKNLGPAGLAFAIVKEELLGKVSRHIPTML
                     DYRIHIENGSMFNTPPVLPIYSALETLRWLKANGGLEAMEKKNIAKAELLYNEIDRNK
                     LFVGTAAKEDRSRMNVCFVMNPEYQELEAEFLKFASDRGMVGIKGHRSVGGFRASIYN
                     ALHIESVQALVGCMQEFEKLH"
BASE COUNT         5204 a         3345 c         2903 g         4975 t
ORIGIN      
        1 caaatcggga cgacgtcgcc gcgttcgttc taccgactgt gcgtgtagcc attgtcggat
       61 attggcttca tgacccgata gcaaaacatc cggaactttc caacctttat aatctgccgg
      121 acgagtataa accggcggcg ccaacaagtt gtcctgaaac gaatcaagca aagccgaagt
      181 ttcatctcca atagcacccg gcaacagacg gacaatagca tcagtcatga tggctgcagg
      241 aagttcaccc cctgtgagca cataatctcc cacactgatt tcgcgtgtaa tcaaatgatc
      301 gcgcacccgc tgatcaacac ccttataatg accgcaaaga atgatgatat tttcacatag
      361 agaaagctga ttggcaattt tttgattaaa catttctcca tcaggcgaag tgtaaataat
      421 ctcatcataa tttctttctt gcttgagttt tgaaattact cgatcgatag gttcaattct
      481 caataccatt ccggcactga aaccaaaagc atagtcgtca acatttcgtc gtttatcgta
      541 actgtaatcg tgcaaattgt gtacgaaaat ctccaccaaa tgcttttcct gtgctctttt
      601 tacaatggaa tgattcaatg gactttccaa caattgtgga acagcagaaa taatatcaat
      661 tcgcattttt cccataaatc gataatcatt ttacattcga gctttccctt tatatttcat
      721 acacgcccat atcctgccct tcaataacct gttgaaaaac ggaagcagct tcattcaaaa
      781 gcacagactg gtcagcataa cgcgctgaat aatgtccgat aatcaatttc cccacttcgg
      841 ctttttttgc aatttcggct gcctgaagtg ctgtggaatg aagcgtctta tctgccctca
      901 ctatatcttc ttccaaaaaa gtagcttcat gatacagaca atctacctga cgaataatgg
      961 gcacaatact ttcgtcgtaa gccgtatcag aacaataggc aaaacgcttg ggagaaacat
     1021 tttcataagt tagtcgctta ttcggaacaa cttccccatc ttcggtaaca aaatcctctc
     1081 cctgcttgat tttatgcaac tgcgaaacag ggatacggta gaaatcgatc atttctcgta
     1141 tcaaatgtct ttcgagcggt ttttcttcaa acagaaaacc acaagttggc accttgtgct
     1201 tcagcgggat ggtcgaaact tttaccgtac gatcctcata aatcagttcg ttcacttgtg
     1261 gattgaatgg atggaaaaac aattgaaagg gagtttcagc agaaaaataa tgcaaaggaa
     1321 cgtccattat tttttccaag tcgggatgag catgtatatg tagatcggct gtacgatgta
     1381 acatggaaaa agaagaaatc agtcccaaca aacccagaca atgatcacca tgcagatgag
     1441 aaataaaaat gtgccccaag cgattggttt tcagtcccat ttgtcgaagt ctgatttgag
     1501 caccttctcc gcaatcaatc aaaaactgct tttcgcgcaa gtcgagcaaa aaagaacttg
     1561 gaaaattttt tttggtaggc aaagccgaac cacaacccag aatagtaaac gttgcttttg
     1621 gcatgattga tttttcaaaa aaagaaaaaa tcttttggca ttttttgctt taacgcttga
     1681 accagaaaat ttaaactttt gttttctttt tcaattcgaa atcgcgtccc agatattttt
     1741 cacggacaat cggattttcg gacaattcaa gagcagagcc ctgaaacatg attcgcccat
     1801 cgaaaagcat ataggcacga tcggtgatgg ccaatgtttc atctacattg tgatcggtta
     1861 tcagaatacc tatgttttta tccttcaatt tccaaacaat atcctgaata tcctgaacag
     1921 caataggatc aactccggca aacggctcgt caagcaaaat aaaacgcggt tcaatagcca
     1981 aacagcgtgc tatttcggtt cttctccgct ccccacccga caaacggtca ccaatgtttt
     2041 ttctcacatg ttccagatta aattcttcta tcaatgtttc caattttttt ttctgatatt
     2101 ctttagtgaa ttttgtcatt tccagcaccg atttaatatt gtcttctacc gtcatttttc
     2161 tgaaaacgga aggttcttgg gccaaatatc cgataccgct ttgtgcgcgt ttataaacag
     2221 gatattttgt tatatcgatg tcgtttaaga aaattttacc ggcgttaggc gttaccaatc
     2281 ccacaaccat gtaaaaagta gtcgttttgc cggcaccatt cggtcccaac aaacctacaa
     2341 tttcaccttg ccgaacttca atggaaacat catctactac ggttcttttg ccgtaacgtt
     2401 ttaccaaatg ttcggtgcgc agcaccattt catcattgct cataactatt ttttgaagtt
     2461 gcaaatttac acttttttat cttgacaatc aaacgaattt ttttcgattg cagcaatcct
     2521 ctgtcggatg ctgtacctaa attgtggtag ataaaataca ttttcaatgg tatttacgtg
     2581 cagttgaaag agaagtaatt ttgctgtata aattctaata aaaaaacatc atgaaagttg
     2641 aaatattttg cccgattaca aataaaatca ccgatgagaa cattgcccga ttgaatgccg
     2701 ggtttacagt tgtgttatta ctgtcttatt ttgtaacttc aaatattgtc ccggtagttt
     2761 tcctgttgct tgatttcact gctcgagcta tagaacgacc cgaatggagt attttgtcaa
     2821 agacgtcaaa atggatatta aaaaagttgc atgtcgaatc aaaattaatc aatgccggac
     2881 caaaaatttt tgcagcgcgc attggcagct ttttcagcct catcacgatt ctgtttttct
     2941 ggctgaagtt accatccatc agcattgccc ttgcagccgt attttttgtc tttgctttgt
     3001 tggaatcggc atttaattac tgtgtagcct gcagaattta tcctttcatt tatcatgctc
     3061 tacaagcaaa agataaagag aaatgaaaaa atatcaggaa tgaagaaact tttctaaaac
     3121 tccttgtcta cacccagttt cttgaatgtt tctttcatga tttcgtcatt atcggcaatc
     3181 aacagtaccg tatctccgat ttccagtttg gtatttcctc gaggaacaaa aaaatgatcg
     3241 ttccgcttaa tcattactgc cagcgtttga tcgggcaagg tgatatccat cagtttacga
     3301 cctttttgca acatttcttc ggtaacagtg atttcgctta tggccgattt aatatcctca
     3361 ggcaaatcta tgtcaaattc cttcaatttg ggtttatctt tggaaggcat ggataatttc
     3421 agcagacgtg caacaaaagg aatggtagaa ccttgaagca ccaacgaaag aattgtgatg
     3481 aaaaaaacta tgttgaacat cattctggct tggggtattc cgctcatcaa gggataagta
     3541 gcaaaaatta tcggtacagc gcctctcaac ccgacccatg aaacaaaaag tttactttta
     3601 aaattaattt ttctgaaagg agaaaggaga ataaaaaccg aaatgggacg cgaaattaca
     3661 atcatgaaca atccaatcaa tatccctgct ccagctatgg gaagaagttc atgaggattg
     3721 accaaaagcc caagcgttag gaacataatt atttgaaaca gccatgttag tccatcaaaa
     3781 aatttaaggc tgcttttttt gtgaacaaat cgctgattgc caataaaaag ccctccaata
     3841 tatacagcca gataaccgtt tccgcccaaa atagaagtaa aactgtaaag aaaaaacaga
     3901 aacgaaagta agagtacaga atataatcct tcataatcca atcgaatccg attgataatg
     3961 aaaatgacca ttttcgataa gatataccca caaatcgcgc caatgataaa ttgacgaaag
     4021 aaaaaggcta ctacttcacc tgaactaata tcggaagatt gtaaaagttg tatgaaaata
     4081 atcatcaaca gataagcaat agggtcattg ctaccgcttt ccaactcaag caaaggacgc
     4141 aagttgtgtt tgatggacaa ttttttggaa cgcagaatag aaaaaaccga agctgaatcg
     4201 gtagaagaca tgatgctgcc caagagcatc gactccaaaa agctgaaagt aatggcagtg
     4261 aaaagatgat ttgtcaacca gtagatgaaa gatcctgtaa taaatgcagt cagcaatacg
     4321 ccaaaagtgg acagaatgat gccttgccaa agaactggtt ttatttccga aaaacgggta
     4381 tccataccgc cggaaaataa aataatattc aaagccacca ccccaaccag ctgagcttgt
     4441 ttgggagaat taaactctat ccccaatcca tcgcttccgg caagaatacc tataagcaaa
     4501 aaaagcaaca acgccgggac tccaaaacga taacccgttt taccggctac aatacttaaa
     4561 ataagaagaa cagaagcaac cagaaaataa aattcaactg caagttccat ttcataattc
     4621 gttgatgttc taacaaactt aagatttttt tcaaagaatt aaagtcgtct atcaaacaag
     4681 ttggtttgtg taccaaaaac ctactgaata caaatggggt tcatcgattt taaaaaaata
     4741 tggaaaaaac ttttcttcaa gattttacta ccttatcgtg gcttttatgt agaaaatcaa
     4801 ataatttttt aagaaaccca catgtattac atacacaaac acgaaggaat ataatttcag
     4861 acatgtgggt tcctcagaac ataaaaataa attatattct gatggaatta acctttaatc
     4921 cgtttttaca gttgtttcaa gaggtttttc atactgactt tttcgttcag aatagcagat
     4981 aatgcttcga tttttacacg ttcctgtttc atggaatccc gataacgaat ggtaacagtt
     5041 tcatcttcaa gcgtttgatg atcgactgta atacagaatg gtgtcccaat tgcatcctga
     5101 cgacgatatc gctttccaat ggaatctttg tcgtcataaa aacatttaaa atcgaatttc
     5161 aaatcttcaa aaattttatg tgccacctca ggcagtccat cttttttaat taacggaaaa
     5221 attccgattt ttacgggagc caataccggc ggaagtttta ataccacacg cgtttcacca
     5281 tcttcgagtt tttcctctgt ataagcagaa gccataatgg aaagaaatgt tctgtccact
     5341 ccgatagagg tttcaatcac gtagggagta taagattctt tcaattccgc atcgtagtat
     5401 ttcaaatttt tccccgaata tttttcgtga tttttcaaat cgaaatcggt acgggaatga
     5461 atcccttcaa tttctttaaa accaaaaggc aattcaaatt caatatcggt agcagcattg
     5521 gcgtaatgtg ccagcttgtc gtggtcgtga aaacgatatt tgtgatcgcc caagcccaaa
     5581 gctttgtgcc atttcattcg atatgctttc caatattcaa accatttcat ttcctcaccc
     5641 ggccgaacaa aaaactgcat ttccatttgc tcgaattccc gcattcggaa aataaactga
     5701 cgcgccacaa tttcatttcg gaaggctttt cctatttgtg caataccaaa gggaattttc
     5761 atccttccgg ttttctggac attcagaaaa ttaacaaaaa ttccttgtgc cgtttctgga
     5821 cgtaaataaa ttttcatggc actgtcggca ttggaaccca tttcggtagt gaacatcaaa
     5881 ttaaactggc gcacttccgt ccagtttttt gttccactta ccggacaaac aatttcattg
     5941 tcgataataa ttgctcgcaa ttctgccaaa tcgttatcgt ttaaagcttt aacatatcga
     6001 cgatggattt catcacgttt tgcctgatgc tccttcaccc gctcattggt agcccgaaag
     6061 aggttttcat cgaaattttc gccaaaacgt ttggctgctt tttctacttc cttttcgatt
     6121 ttttcatcat acttggctat caattcctca atgagaacat cggcacgata acgtttttta
     6181 ctgtctttgt tgtcaatcag cggatcattg aaagcatcca catgtcccga agctttccaa
     6241 gtggtgggat gcataaaaat tgcggcatcg atgcccacaa cattttcgtg aagcagaacc
     6301 atactgtccc accaatattt tttaatattg tttttgagtt caacgccatt ttgtccataa
     6361 tcataaacag cgctcaatcc gtcgtaaatt tcgctggacg gaaaaacaaa tccatactct
     6421 ttgcaatgag caatcagttt cttgaaaaca tcttcttgtg acagcatatc aacattgttt
     6481 taattatcaa tcctttaaat cgtaatccaa tacatttttt aacataaaac tttcccccgt
     6541 aaaaaatagt ttcaaaggat aaaagtttta ccaaaagaaa tgcaaaatta cttctttttt
     6601 tatatttttg ttgattcaaa cagacaaaaa agttcattta ttccctaaat tcatgctgaa
     6661 aatcaacaaa acaaatattt ttcctcctca catgatacaa aacacaagta ttttcttcac
     6721 acaaaaattg ttgcaaaggc ataaatcaaa agaattcgtt ttgataaagt ctatgttggt
     6781 agcatggatg atgattgctg ctttttcatt aaatgcacaa gatatcattc tgcaaaccag
     6841 gacattgaca acttccactc acgcctttgg attttctacc atcaacatgt tggattctta
     6901 cctttcacca ttgaaataca gtggatcggg atttcgttat gaaaacgaca acagaaaatt
     6961 tgtttcgcct gaaaatattc gtctttcaaa agaaaatcat ttcaacatga attttggttt
     7021 gaccgaaaac ccagctcaaa ccagcgaaat gtggtatttg ggaatgaatt tttcnnnnnn
     7081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgaccg aaaacccagc tcaaaccagc
     7201 gaaatgtggt atttgggaat gaatttttcg tgggggatgc atcgacattt ttatgtcgga
     7261 gagaatcttc tgctgctagc cggaggtctt tgggatttgg actttatggg gaaaaccaat
     7321 agtcgcaatc aaaacaatcc cttcaatatt gatcttgcta ccaacctgaa tctttcaggg
     7381 gaagccgttt atcgaatcag aacgcccaaa cgcatgctcc gtcttggatt ttccatgcaa
     7441 gcgcctttgg tcggagtgat gtttgtgccc gaacagggag cttcgtatta tgaaattttt
     7501 gaattgcaaa atttttcaaa tacattgcat ttcacatcgt tacacaataa actgtcactc
     7561 accgaaaatc tctcgctgga tattcctttt aatcacacaa ctttacgttt tggctttaga
     7621 aacgaaaatt taaagtttga agccaatcaa atgatattta aaagaaacga tttaagtttt
     7681 catgtcggat tcatttacga tttttacaag tttgaaggaa caaaaaatcg agcgccttca
     7741 agcttcaaca gtacttataa ctactaatca acaagttttt tcatgaaaaa acatatcgtt
     7801 ttgctttcac tctttggaat gctttttcat tcgtgtatgg atgagcccgt cattcaacac
     7861 aatacttatg aaggaaattt taatgcctta tgggaaatta tcgataccaa atactgctat
     7921 ctcgattata aaaatattaa ctgggattct gtctacactg tttatcatca aagaattccg
     7981 tacataaaaa cagatttgga tttttttgat cttctcggca aaatgctgga agaattgaaa
     8041 gatggacacg taaatttata ttccggtttc gatgtcagcc gttactggaa atggtacacc
     8101 gattatcctg aaaatttcag ttcggatatt atttattcca atcgatattt aggtgaaaac
     8161 tacagaattg caggcggaat gcattatcaa aaaatcgaca atggtaatat cggatacatt
     8221 tattacggag acttcaccag cgatttcagc gatacaaaca tgggctacat attcaaatat
     8281 ttttccaatt gcaagggaat tattctggac gtacgaaaca atggtggcgg atacatgagc
     8341 ggagctgaac aattggcttc ctactttttt actgaaaaaa cgctcaccgg ctatctttgt
     8401 cataaaacgg gaccgggaca taccgatttt tcgactccga taaaattgtt tactcctgcc
     8461 aacaaaaatg ttcaatggtt gagaccggtt gtgattctta ccaaccgcat gacctacagt
     8521 gctaccaacg atttcgtcaa cagaatgaga aaagcgcctt atgccattat tttaggcgac
     8581 aaaacaggcg gcggcggcgg aatgcccctt tcaagcgaaa ttcccaacgg ttggatggtc
     8641 agagtgccta cttcgcccat gttcgacgcc aacatgcaac atattgaatg gggtattgac
     8701 cctgatataa atatagcctt aaagccggaa gatgccgcaa gaggctacga tacactgatt
     8761 gagagagctg tagcgttgat taaattttta tgaaaaatcg aaaattgaaa taaataatcg
     8821 aaaataaaat ttttgtttga gaaaatgcaa aagctgaaag ttacggaaat gaatcgcttg
     8881 acagtagagg aattcaaaaa aaaagaaaaa acgccgctga ttgttgttct cgataatgtt
     8941 cgcagtcttc acaatgtagg ttcaattttc cgcacagcag atgcttttct ggtacaggcg
     9001 gtctatttat gcggaataac cagtacgccc cctaatgccg aattacacaa aacggcattg
     9061 ggagcagaaa attccgtaga atggaaatat ttcgaagaaa cgcataaggc cttggaagaa
     9121 ttgcgaatga tgggatatac aatttttgcc atagaacagg cacgtgaaag tttaccatta
     9181 aattccattg aattgaatcc ggcaaataaa tatgccattg tattggggaa tgaagtgaaa
     9241 ggcgttcaac aaacggtagt tgatgcctgc gatggttgca ttgaaatatt acaatacggc
     9301 accaaacatt ccctcaacgt aagcattgcc gggggcattg tagtttggga gttttttaaa
     9361 aaattgatgg aagaaagaaa ttaattccct ttcagtttct taaacgggag tttaacttac
     9421 tccaaagatc tttcgaaatt agttggtttt aggacgaggg tataaaatga atgaggaaaa
     9481 tcgctgatag taacaagaaa tgaagaaacc tgtgcccaaa catcgcaaca tggattggct
     9541 aatatttaga gagtccattg aaaagcacta ccaaatatca tacaaaaagg ctcagattta
     9601 taatttgcta ctttataggt caagattttg agaaaaatac ttcgcacatt taaaaaacaa
     9661 ttcaaagatt attttgatag ttgagaatga gtatcaccac gcaaggcttc tgaaaaaatt
     9721 tttgaactca aacaaaaaat ttacattttt ttgtttcgat acttactcca tccaatttca
     9781 attcaaaaag cccggtgatt cataagaaaa tcgataattc acattatgcc tttagaatcg
     9841 ctgaagttga gtaaagcaaa gcaaagctta atcagaatag ctactcattt tcaaattcca
     9901 aacgaagaat tattgactat ttgtgcaatt tattattaaa caaaatatta aaaagcaaaa
     9961 gaggcaaaac ctatttttgg tttcacctct tccgaaaaac aaattggaac taatatttat
    10021 ctgattgtca actcataagg caaacgagcc tgaagtccat ccattttatc gagctgctct
    10081 atcattttaa acatttgata atgttctccc aattttagtt tttcgatgcg tgtttccgtt
    10141 ccatccgaca attctttcag tagtttttcg ctgaaagtct caattttagg aaattcctta
    10201 atttggtagg aagtcagctt tgcttttttc actgccatag aaatggcatc gtttattgtt
    10261 cccaatttgt ccaccagtcc gatttccatt gcatcttttc ctgtccaaac gcgtccttca
    10321 gcaatggctt taatttgttc aaccgacatt tttcttccat cagcacaacg ttttacaaac
    10381 aattcgtagc cccgattcac ataattctgc attaaatctc tttcttcggg tctgaaggga
    10441 cggttaatac ttattgcatc gctcatttta ttggttttta caccgtcaaa agttaatcct
    10501 attttcttgt ctaacccttc aatattgggg ataagcccaa aaataccaat agaacccgta
    10561 atggtatttg gctgggctac aatgctgtcg gctatgcagg aaatataata accacctgaa
    10621 gcggcataat cgcccattga aacgattaac ggtttctttt gcttcaactg acttaaggca
    10681 tgccaaattt gttcggagcc gtaagcactt cctcccggag aatttactct gaaaacaact
    10741 gcttttactg aattatcttt tgctatttcg ttgatggttt tcaccaattt ttttgactga
    10801 attccgtcag atccgttgtc aattgcgccg gctgcataaa gaactgcaat tttgtttcga
    10861 tcatatttgg tagcatcggg aactttacac atggcttcat gcccaacata atttatgttg
    10921 gcagttgaat cctttaaaaa acctttgaca atgaagtcga tttcatcctg atatacgagt
    10981 gaatcgacca aagcatactg cttggctttt tctgtaggtt gaaaagtcag catttcatcc
    11041 gcatagacat ttaatttttc aaaaggaatt ttacgactgt cggaaacttc cgagagcaat
    11101 gtattccaaa tggaattgag ataaacggta acctgttcgc ggttagcgtc gctcattttg
    11161 gtgttaacat aaggttcaac ggccgattta aaagttccaa ctttcactac ctgcatttcg
    11221 atgcccagct tgtcaagcgt atttttataa aaagttatct cggaggccaa gcctttccaa
    11281 tcgagcatac cctgtggatt cagaaaaatt ttgtcggcaa ccgaagcgag gtaatattcg
    11341 tgttgcagat aattgtcggc ataagccaca acaaattttc cggatttctt aaaatctttc
    11401 agcgcatccc gtatttcctt catggaagca tatccggcag ataaagtacc gaaattgagg
    11461 taaataccgg caatattttc atctgttttg gctttttgaa tattactgag aatatcgttc
    11521 aaaccaatgg tgaggttgtc ttcatttccc atcgcttgtg ccaaggcaat ggagaaaggg
    11581 tcttcttccg aacgttctac caaaactcca ttcaatttaa gttcataaac ggaattaggt
    11641 tttaattttg ttggagcact tttttgtccg gctattccac cggcaattcc aaacagaatg
    11701 gctaccaaaa taataccggc caaaattaca cccaaaatag tggcaagtgt gtacttaaaa
    11761 aattgcttca taaataatga ttgttttgat attaatatgg ctaagaaaca attgataaaa
    11821 aaggagaaaa atcactgaca aaaatagagt ttaaagaaaa aatacaagct atttctcaat
    11881 gccgaatacg agtaaattaa caagttcata aaaaacttta caaaggaaat caataatttt
    11941 ctcatttcaa acgattgaat tcaaattttt tttgaagcag caaaattttc taccctttca
    12001 gcaaaatgaa aaagagaagc cttcttttaa caaaaaggtt cctcttctca aaaaaattat
    12061 gaaaaatctc tcgaaatcaa ttatttgaag tcatcccagt tggttcccaa catgcttccc
    12121 gttttttcga gctgaatgtg catgccgaaa caggctttca aattgttggg cgtgtgactt
    12181 gatccggaaa tggcaagata atcgcgcagc atttgcttat attcaggtgc aacacagttt
    12241 ttgataatga tttcagctcg ctctttgggc gttgttccac gtaaatcggc aatcccatat
    12301 tcagtaatca gcacattcac agaatgttcg gtatgatcca catgagatac catcggaaca
    12361 atgctgctga tatttccgtt tttggcagta gaaggagtgg taaatattga aataaacgag
    12421 ttacgtgtaa aatccgcaga accgccaatg ccattcatca ttttagtgcc aagaacgtgc
    12481 gtgctgttga catttccgaa aatatcggct tcaagcgccg tattgattgt aatcaaaccc
    12541 aaccgacgca ccatttctgg attattggaa atttcctgtg gacgcagcac caatttatcc
    12601 cggaaaaaat caatattttg ataaattttt tgcaataccg catcggtaac tgtcaacgag
    12661 caaccactgg caaattttat gttgccttcc agcatcaatt cgatgacggc atcctgtatt
    12721 acttccgtgt acatttcgaa gggtggtatc tcttcatttt tacccaaaga aaacagtaaa
    12781 gcattggcaa tgttacctac tccggactga ataggtaaaa aatcatgggg tagccttccg
    12841 gcacgaattt caccagccag aaaagtagcc acattgttgc caatttgctg ggtaacctca
    12901 tccaatggcg caaaatcgga aacttcatca ggtaaattgg tttcaacaat acaaagaatt
    12961 ttcgctggat caactttcaa aatttccgaa ccaattctgt cggatggttt gtaaatggga
    13021 atttctctgc gataaggcgg atcctgaggt tgataaatat catgaaaccc tttcaactct
    13081 ttgggatgat aatgattcca ttccagaata atatgatcag ccatttcggc aatagtgggt
    13141 gaaatgccta cagcagctgt taaaaccagt tccccgtttt cattccagtc gcaaacttcc
    13201 aaaatggcaa attttggctt tggcaaaaat ccaaaacgca attcctgagc cattgtggac
    13261 aagtgcaggt caaaataggg agctttccca gcattgatgg cgttgcgcat gtccttattc
    13321 gactgataag gagttctgaa caacacagca tctgcttttg ccaatgcacc atctaccgat
    13381 actcctgttg aagcgcctgt atacatgcct attttaaatt cttttccttc tgcatgaaaa
    13441 gtttctgcgc gcctggctat agctacggga acagctttgg ggcagcccgc aggcgtaaat
    13501 ccgctaaatc ctactacatc gccgtgttga acataagttg cagcttcttc tgcactgaca
    13561 aacttaaatt tattttgcat aaaaattggt ttatttaatc ttgaaaattc gccgtaaaat
    13621 taacaaatat ttctcaatcc ttcaagaatt tcagtgtcat gggcactaag tttacttttt
    13681 tataatttct ctaagcacat gacaaaattt taaaatatcg atatttgatt gtcaaaagtt
    13741 ccagttcgtt ctgtcgatag cactcgagta cggtggatca tgcagtagaa gcacaaatat
    13801 caaatgagca attaagtctt aaagtttgta atcagccata aaagccgtat tcgccgcaat
    13861 gaaaaattgc tgtcgcagct gttttgaagc caaaggcgag cttttcaaaa caaacgacct
    13921 gaaaccttac aaacggcgca aataaagagg ctaataaact ttattcgggc taagtttata
    13981 cctttaccga agtgttgaac caaaactgaa gttaaatcac tacttttgga atagaccaaa
    14041 gactttataa taaaaaagtg tcctttgagg acaccatctg gtcatttaaa tcaatacaag
    14101 attatttttt atttacgcgg aaacgttcat ctcccgtttt gaagaacgac acaatttgct
    14161 tggcggcagc aatgccggca ttaatgttgg cttcggcagt ctgagcaccc attttcttgg
    14221 gtgtcgaaaa ataacgtacg ggaaattttt cggcaaaaac tgcatgctga tcaggcataa
    14281 tgtcggtaac atatttaata tcattacgca actccatgca atttatcaaa tcttcttcgt
    14341 taatcacttc cttgcgggcc gtattgacta tgactgcacc ttttggcaac ttctgaagca
    14401 aagaagcatt gatggatttt tttgtttcgg gagttgcggg gatatgaatg gaaaccatat
    14461 cgcatgaagc atacaactct tcaacagaag tgagtggagt aacgccatca tttttaataa
    14521 cttcagcggg acaataagca tcatatgcaa aaatttgcat atcgaaacct tttgctattc
    14581 tggccacatt tctgccaaca tttccgtaag catgaatacc aagttttttc cctttcaact
    14641 ctgttcctga agacccgtta taaaaattac gcacagcata aatcaacagt ccgaaaacca
    14701 actcggcaac tgcattagaa ttttgccccg gcgtattcat gacacaaata tttgcctggg
    14761 tagccgcatc aaggtcaata ttatcataac cggctccggc ccgaacaaca attttcaatt
    14821 ttttggctgc ctttatcact tcagcatcta caatatcact gcgaacaatc aaagcatcgg
    14881 catcggccac ggcatcaagc aactgttgct tttcggtata tttttcgagc agggcaagtt
    14941 cgtatcctgc ggcttccact tctgcccgaa ttccatccac tgccactttg gcaaatggtt
    15001 tatcggtagc tacaagtatt ttcataagca aacttttttc tctccttttt caataaagaa
    15061 gaattattta tgttaatggt taaaattgta aacagctgtt gctaccaaaa gcatcaatgc
    15121 aatttttcaa attcctgcat gcagccaacc aatgcctgaa cgctttcgat atgaagtgca
    15181 ttgtaaatgg aagcgcggaa accgccaaca gaacgatgcc ctttaattcc taccatacca
    15241 cgatcggaag caaatttaag aaattcggct tccagttcct gatattcagg attcataacg
    15301 aaacaaacat tcatgcgtga acgatcctct ttggctgctg ttccaacaaa caatttattg
    15361 cggtcgattt cattgtagag caattcggct tttgcaatat ttttcttttc cattgcttcc
    15421 aaaccaccat ttgccttcaa ccagcgcaac gtctcaagtg ccgagtaaat gggaagaacc
    15481 ggcggcgtat tgaacatcga accattttct atatgaattc tgtaatcgag catagtgggg
    15541 atatgacgag aaactttgcc tagaagttct tcttttacaa tggcaaaagc caaacctgca
    15601 ggtcccaaat ttttctgtgc acctccataa ataacgccat attttgaaac atctacagga
    15661 cgtgaaaaaa tatcggacga catatcggca accaaaggca ccggagaatc gatgtcggtc
    15721 aaaatttcgg ttccgaaaat tgtattattg gttgtaatat gaaaataatc gacgtctgag
    15781 gggatcacat aattttttgg aatatagctg aaattggcat ctttggagga agcaacttct
    15841 attacttctc cgaaaccttt agcttccttg taagccttgc tggcccaagt accggtaagc
    15901 aaataagcag ctttcttttc aagcaggttg aagggaacca tgcaaaattg caaacttgcg
    15961 cctccaccca aaaaaactac cgaataaccc gaaggaattt ccaacaattc tttaaaaaga
    16021 gcaatggctt catccattac tgcctgaaaa tccttagaac gatggctaat ttccaaaatc
    16081 gacaatccgg aaccattgaa atccaatacc gcttgtgctg ttttctcaat cacttcacga
    16141 ggtaatatcg atggccccgc attaaaatta tgctttttca tcatttcatt aaaaaatttt
    16201 tttggtttat aagtttctcc ctttgaattt aaagcacaaa agtacacaaa ttttaaattc
    16261 tttcatttct tatggaaaaa ttttttagtt tcaattttaa attttcatat aggagtctcc
    16321 tctgaaaagt tactcaaaga gaattagttt tttaaaaaca ttgagataaa aaaggtaaaa
    16381 aatattcaaa aaagttcttt gaaatttgca tatattgtta tatttcagta tcttaggagg
    16441 ttgaaatacg ataaaaagag agataaatgt tcaggaaaac agaaagcgaa atacaattag
    16501 atatattttt ttcgcccgaa aaactta
//
LOCUS       DAOSCH010000044        16448 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2425_length_16448_cov_5.744220, whole genome shotgun sequence.
ACCESSION   DAOSCH010000044 DAOSCH010000000
VERSION     DAOSCH010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 16448)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16448)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16448
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2425_length_16448_cov_5.744220"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..471)
                     /locus_tag="PLZ54_04870"
     CDS             complement(88..471)
                     /locus_tag="PLZ54_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66296.1"
                     /translation="MKRKLFILGLMIFTAISLSAQLPKFMELSPEVRAKTHTEWMITT
                     LKLNPNLSEKIYSINLQFAKEVEPVKSDEISKFKKIKIIRNADGERLKQFKTFMTPDQ
                     VETYIQKRKELFDEERKREKESAKN"
     gene            complement(639..1340)
                     /gene="tsaB"
                     /locus_tag="PLZ54_04875"
     CDS             complement(639..1340)
                     /gene="tsaB"
                     /locus_tag="PLZ54_04875"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOJ66297.1"
                     /translation="MNLPLILHIETSTQVCSVALSSGTECVFSKSSEEQGMNHAKLLS
                     GFIAEAMNVLNNDFHSLNALAVSSGPGSYTGLRIGVSTAKGLCFGLDIPLISIGTLDI
                     MAVSALNSVNEKDALLCPMLDARRMEVYAAVFNTDLEMIQPVAANIVDERTFNEILKQ
                     NSVYFFGNGMSKCKPVLQEHARAHFIDEIIPLAENMIPLALKAYQKKQFVDTAYFEPF
                     YLKEFQTTVSKKKMF"
     assembly_gap    1526..1625
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1876..2043
                     /locus_tag="PLZ54_04880"
     CDS             1876..2043
                     /locus_tag="PLZ54_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66298.1"
                     /translation="MSDNGDLLYKSKDGNLLQINHYDTRQLKLTVRYNFNTTKSKYKG
                     TEAGEKEMKRL"
     gene            complement(2069..2590)
                     /locus_tag="PLZ54_04885"
     CDS             complement(2069..2590)
                     /locus_tag="PLZ54_04885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ66299.1"
                     /translation="MKFVRGLFFALIVLCFLNGCRKTPPQLPANKNLDTTNNEVVALV
                     ELNEKMALKEDSVLKQFVKKLKGNFVKSPVGIWYFIENHTKGSTLKNSDSCFISWQMF
                     SLEGKLLGEKSHEKVIIGKKQIPVGLEEGLRLMHKGESAVFVVPWYLAYGMKGLPPDV
                     PPYTSLIYKVKFE"
     gene            complement(2594..5029)
                     /gene="thrA"
                     /locus_tag="PLZ54_04890"
     CDS             complement(2594..5029)
                     /gene="thrA"
                     /locus_tag="PLZ54_04890"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HOJ66300.1"
                     /translation="MKVMKFGGTSVGSTEAVLNVKKIVESQKESVIVVVSALSGVTDQ
                     LYRISQLAASGNETYLDEYNQMFSRHMQMIAETVLPEKQAEVELQIQEIFTDLLNIFR
                     GIFLIKDTSVKITDTVVSYGEMISSVLIANIIQGAVHFDSRRFIKTQNQFDKHIVDFK
                     TTNELIQQIFKDAPSIVVCGGFIATDVHTNQVTNLGRGGSDYTAAILAAALNASILEI
                     WTDVDGFMTADPRIINNAYVIDRLSYSEAMELSNFGAKVIYPPTIFPAFHRNIPIVIK
                     NTFRPEASGTYISQEKSGEKSKAIKGISSINDTSLITIRGLGMVGVIGVNYRIFRTLA
                     KNGISVFFVSQASSENTTSIGVKNADSALAVEVLKKEFANELELGEIDEIFSEDDLAT
                     VAIVGDNMKRTPGIAGKLFGTLGRNGINVVACAQGASETNISFVTDRSSLRKALNVIH
                     DSFFLSEYQVLNIFVVGIGKVGSHLLHQIKQQQPKLMHQKGLKLNVVGIASSKKMLFS
                     REGIDIEHYQDELKLHGVDSTPERIADEIINMNIFNSVFVDCTANAQIADLYQTLLDH
                     NISVVAANKIATSSSYDKYMKLKETARQKGIKFLFETNVGAGLPIISTMNNLTNSGDH
                     ILKIEAVLSGTLNFIFNTLSSDIPFSKAVLMAKEYGYSEPDPRIDLSGKDVVRKLVIL
                     AREAGYQVEQDDVKKNLFIPEKFFKGSLEQFWEQIPELDEAFELRRQELEKQGKRLRF
                     VASMDKGDCQVGLQEIDNSHPFYDLQGSNNIILLTTERYNEFPMMIKGYGAGASVTAA
                     GVFSDIISIANIR"
     gene            5184..5257
                     /locus_tag="PLZ54_04895"
     tRNA            5184..5257
                     /locus_tag="PLZ54_04895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5218..5220,aa:Arg,seq:tct)
     gene            5720..6409
                     /locus_tag="PLZ54_04900"
     CDS             5720..6409
                     /locus_tag="PLZ54_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOJ66301.1"
                     /translation="MLIFVTPLHQYLPGYGDMNLRSKIIQESIRADSLTHAMNLQAAY
                     LEIVKDIIKGNIKPDSVVGVDSAFLQQQAQKLIETSKREKEFTKKFEEEEKFNLTSME
                     SSSTNKKTYVFFRPATGNIVSSFNISKKQYGVALQTLPDAAVSSVLNGIVVFADFTFD
                     NQWVIAIQHEDNYLSIYKNNNRLLKNIGEVVRTGEPIAITGSSKGKNGQLYFELWQQG
                     KPINPQNIIIF"
     gene            6455..7627
                     /locus_tag="PLZ54_04905"
     CDS             6455..7627
                     /locus_tag="PLZ54_04905"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOJ66302.1"
                     /translation="MSNTTKQIAVLGSTGSIGKQALQVITDYPDRFSVYALTANNNID
                     LLIKQARKFQPEIAIIANENHFLQLKEALSDLPIKVFAGEESIAQLVEMQAVDIVLIA
                     LVGYAGLKPTMNAIKAGKRIALANKETLVVAGELIEEMVACYHPSIIPVDSEHSAIFQ
                     CLNGEGEKTEVEKIILTASGGPFRTKTSKELESVTIAEALKHPNWEMGTKITIDSASM
                     MNKGFEVMEAKWLFGVAPEQIEVVVHPQSIIHSMVQFIDGSIKAQLGVPDMRLPILYA
                     FTYPERILTTFPRIDFNLLNSLTFEKPDLERFHNLALAYYALDKGGNMPCALNAANEI
                     VVDAFLNSKLRFLQMSEVIETILSKTFYIGHPSYEDYVQTDIITRQITRELIAQVH"
     gene            7752..9242
                     /gene="rseP"
                     /locus_tag="PLZ54_04910"
     CDS             7752..9242
                     /gene="rseP"
                     /locus_tag="PLZ54_04910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOJ66303.1"
                     /translation="METFLIRALQLILSLSILVVLHELGHFAFARLFKVRVEKFYIFF
                     NPGFSLLRAKKINGKWQFKFFSKNVPSNERPKKDAEGDIVMGEKGKPQTEPIPLSELS
                     DDDWRKYPESTEWGIGWLPLGGYCKISGMIDESMDTSQLKSDPQPWEYRSRPVWQRMP
                     IITGGVLVNFILAMVIYSAILFTWGKEYLPLENAKYGLHFTSVFIEQGFKNGDKILSV
                     DGKSCADLGEVIEKILIDNAKSVTVLRGDSQILTISLPPDFPQKVLASGESNLISYRF
                     PFVVQDVSPESPAKQAKLQPGDSIVGINGKELTIFQDVVSVLTNYRDSLIQLAYYRNG
                     QKEQATVQLDQTGKLGVAVRPFTEFLETEHVKYGLLASIPAGISLGWETLTSYIKQFK
                     LVFTKEGSKQLGGFGSIGKLFPQTWNWQIFWSMTALLSVILAFMNFLPIPALDGGYVL
                     FLLYEIFTGKKPSDKFLEQAQTIGMLLLLALLIYANGNDIYKAIFK"
     gene            9371..9526
                     /locus_tag="PLZ54_04915"
     CDS             9371..9526
                     /locus_tag="PLZ54_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66304.1"
                     /translation="MARVLRVTANLKEAISKADVLMNRNLIEGSNERVTEQWIGNARN
                     STVTQME"
     gene            complement(10034..11344)
                     /locus_tag="PLZ54_04920"
     CDS             complement(10034..11344)
                     /locus_tag="PLZ54_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOJ66305.1"
                     /translation="MSISKDLVDEIFRQFGQEDINKLSIREMGKLVAQIEQKTNVEFI
                     HMEMGVPGLPPLQTTVDAEKEALDRGLAALYPDIEGIPFVKNETARFAKLFMDIDVTP
                     LNCIPTVGAMQGSFAALLAAARTNKNKPCTLFIDPGFPVNKSQLQLMGLPFKSFDILE
                     NRGDRLRSALTSFLKEGDICTITYSSPNNPSWINFTEEELQIIGELATEYDVIVVEDL
                     AYFGMDFRKDYGKPGVPPYQPTVARYTENYILLLSASKIFSYAGQRIGMMIISESLRT
                     RHYPELKNYFTSDVLGHFIPYGALYATTAGTSHTAQYAFAATLKAVNDGKINFLEALK
                     EYAEKAKIMKEIFLKNGFYIVYKEKNEELADGFYFTVAYPEMTSSQLMKNFLYYGLSA
                     ISLSITGSKDNHGLRICVSFVRPDQIPVLDKRLKEFAEDMKKNV"
     gene            complement(11364..12662)
                     /locus_tag="PLZ54_04925"
     CDS             complement(11364..12662)
                     /locus_tag="PLZ54_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOJ66306.1"
                     /translation="MIWNEEIECMSSSEMRKLQSERLKELVNKVYQNVEFYRKKMDEK
                     GVRPSDIRSIDDISKLPFTNKTDLRDNYPFGLFAAPMKEIVRIHASSGTTGKPTTVGY
                     TKKDIENWQEVLARCLTMSGVTPEDIVQVSFGYGLFTGGLGIHYGAENVGCTVVPISA
                     GNTRRQLQLMSDFGSTVLACTPSYALHLADALSEFGYSLSDMKLKTGVFGAEPFSENM
                     RLELEKKWGIKAFDIYGLSEIMGPGVANDCEFHDGLHIHEDHFFPEVVDPKTKEPLPN
                     GQQGELVFTTLTKEGIPLLRYNTHDLTSLNREKCECGRTLVRMKKITGRSDDMLIIRG
                     VNLFPSQIEQVLLEMGETSAHYMLYVDRENNLDILELKVELDETKMVDTIKDLMSLSR
                     KIEHSLHSALGLSVKVTLVEPKSIPRSEGKAVRVIDNRKI"
     gene            complement(12659..13087)
                     /locus_tag="PLZ54_04930"
     CDS             complement(12659..13087)
                     /locus_tag="PLZ54_04930"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HOJ66307.1"
                     /translation="MKIRQLSVFVENKTGHLNQILAVLARNNINIIALTLADSIDFGL
                     LRMLVSDAEKAAEALRTENFTVRIHEVLSLELEGVPGSLSRVLNHFSDNGIAIEYIYA
                     FSFGSKSILVMRPDNTEKAIEIIQKYQLKPIYESDLKAQL"
     gene            13325..14275
                     /locus_tag="PLZ54_04935"
     CDS             13325..14275
                     /locus_tag="PLZ54_04935"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOJ66308.1"
                     /translation="MNNLGKSNTPFFFLLDFMAQNGYIIKNQDLDDQYIRFQLSAESF
                     SNQKKSTKNSFNFQWNIHPVNFDEYQLKFHFVMENIRCGNSFLLNLTQPTEVSTNLNL
                     LELYELSVSKYKLWLKDLFTVFSPETFIKIEDGIISTFPMKGTIDASVANAEDVILND
                     FKEQAEHATIVDLMRNDLSMVAEKVEVKKYRYIDKIITNQNELFQTSSEITGELPENY
                     CCYLGNIIISMLPAGSISGAPKRKTLEIIREIEGYDRGFYTGIFGQYDGKNLDSAVLI
                     RFIEQKNGQMIFKSGGGITSNSEVLKEYREMLEKVYVPIY"
     gene            14259..14855
                     /locus_tag="PLZ54_04940"
     CDS             14259..14855
                     /locus_tag="PLZ54_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOJ66309.1"
                     /translation="MYRFIESIKLLDGIFYRLKFHQKRMNICSELFYPNDNFIHLQSA
                     LTDSHPPQKGLFKCKITFESKILKIEYLPYKKKEINSLRIVHTEIESRPYKKEDRTDF
                     NAAFDMRGNYDDVLLMKNGLLTDTSICNIALFDGFSWQTPTKPLIYGVNRANLLEEGK
                     IFEKDIKLSDLQNYSAIRLFNALIEFGEIELNISNIHF"
     gene            15193..>16448
                     /locus_tag="PLZ54_04945"
     CDS             15193..>16448
                     /locus_tag="PLZ54_04945"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOJ66310.1"
                     /translation="MDRLFIFDTTLRDGEQVPGCQLNTVEKIEVAKALENLGVDIIEA
                     GFPVSSPGDFNSVVEISKTVTWPVICALTRAVEKDIEVAAEALQYAKKKRIHTGIGTS
                     EYHIRYKFNSNQEEILERSVAAVKYAKKFVEDVEFYCEDAGRTENEYLARVVEAVIKA
                     GATVVNIPDTTGYCLPWEYGEKIKYLMEHVDGIHKAIISTHCHNDLGMATANTISGIM
                     NGARQAEVTINGIGERAGNTALEEIAMILKCHKKLNIETQINTKKIYSTSRLVSNLMN
                     MPVQPNKAVVGRNAFAHSSGIHQDGVLKNRENYEIMNPEDVGIDHNSIVLTARSGRAA
                     LKHRLNTLGVELADKKLDAVYEEFLKLADKKKDINDDDLLMLAGKERSEKERIKLDYL
                     QVTSGVGVKPVASIGLIIGSEKFEAAS"
BASE COUNT         5355 a         2994 c         3029 g         4970 t
ORIGIN      
        1 taaacgggct gcttaatata aaaaagaaag tgtccttttg ggacaccctc tttttggata
       61 aataaaaaat caaattctgt caattattca attctttgca ctctcctttt ccctctttct
      121 ttcttcatca aataattctt ttcttttctg aatataggtt tcaacctgat cgggcgtcat
      181 aaaggttttg aattgtttta acctttctcc atcagcattt ctgataattt taattttctt
      241 aaacttgctt atttcatccg atttgacagg ttcaacctct ttggcaaatt gaagattaat
      301 ggaataaatt ttttctgaca aattgggatt aagttttaaa gttgtgatca tccattcagt
      361 atgagtttta gctctaactt caggtgataa ttccataaat ttaggtaatt gagctgaaag
      421 cgagatagct gtaaaaatca tcaaccctaa aataaacagt tttcgtttca tgtctttaaa
      481 aattttaagt gaatccactt agttttttat caaatttcat tccattttta aattcgaatt
      541 gtaaaggcaa tttttaggat gttgcaatat gcaaaataga agcgtggaga ttcttgccga
      601 tgagctgaat ttttaatcga caaaattggc ttataatttc agaacatttt tttctttgat
      661 acagtggttt gaaattcttt caaataaaac ggctcaaagt aggctgtatc aacaaattgt
      721 tttttttgat aggcttttaa tgccaatgga atcatatttt cggccaaagg aataatttca
      781 tcaatgaaat gtgcacgtgc atgttcctgc agcacaggtt tacatttact catgccgttt
      841 ccgaaaaaat atacggaatt ttgtttgagt atttcattaa aggtacgctc atcgacaatg
      901 ttggcagcga caggttgaat catttccaaa tcggtattga aaacagctgc ataaacttcc
      961 atccggcggg catcaagcat aggacataaa agtgcatcct tttcatttac cgaattcaaa
     1021 gccgaaacag ccattatatc cagcgttcca atcgaaatta acgggatatc caacccaaaa
     1081 cacaaacctt tggccgtaga aacaccgatt cgcaaacctg tgtaagaacc cggccctgaa
     1141 cttactgcaa gtgcattcaa tgaatggaaa tcattattca gtacattcat cgcttcggca
     1201 ataaatccac ttaataattt tgcatgattc atgccttgtt cttcggaaga ttttgaaaaa
     1261 acacattctg tgccactact caaagccaca gaacaaacct gtgttgaagt ttcaatatga
     1321 agaatcagcg gtaaattcat aattttatat tgatgatgaa aaaacagcat cataaactgc
     1381 cgttttctgt tggcaaaagt agtaattttt tgctgtagca agaaagattt tcgttatcaa
     1441 gctatataaa atatcactac tgcccgataa aaaattggaa taggttagta taaaaaggga
     1501 agttaccttc aaatttaata acctcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnaaaat tggaataggt tagtataaaa agggaagtta ccttcaaatt taataacctc
     1681 ccctgtggca acattagttc gaatagattt gatcagggaa acaaaagagc tttttgataa
     1741 gaatcagaaa gcttcgcttt tcacgggtca gttaaactta aacctgcaat tttgaaggaa
     1801 ggtctgtttt gaaaattaaa acaaaaaagt tatattatag gggttacaag tataagtatc
     1861 gactgaataa aaattttgtc agataatggt gatttgctct acaaaagcaa agacggtaat
     1921 ttgcttcaaa tcaatcacta cgatacacgt caattaaagt taactgtccg ctacaatttc
     1981 aatacgacaa aaagcaaata caaaggaact gaggccggag agaaagaaat gaaacgattg
     2041 tagaagtgta caggaaaatc ttttattatt actcaaattt tactttgtaa atcaacgaag
     2101 tataaggagg gacatcgggc ggaagtcctt tcattccata agccaaatac cagggaacaa
     2161 caaaaacagc gctttcgcct ttatgcatca aacgcaatcc ttcctccaga ccaacaggaa
     2221 tttgcttttt cccgataatt actttttcat gtgatttttc gccaagtaac tttccttcga
     2281 gtgaaaacat ttgccaagaa atgaagcaac tgtccgaatt tttcagtgta gagcctttag
     2341 tatgattttc tatgaaatac caaattccaa caggtgattt aacaaaattt ccttttaatt
     2401 tttttacaaa ttgttttagc acactgtctt ccttcaacgc cattttctcg tttaattcga
     2461 ccaacgccac aacttcatta ttggttgtgt ccaaattttt attggcgggc aattgaggtg
     2521 gcgtttttct gcaaccgttc aaaaagcaca aaacaattaa tgcaaaaaat aaaccacgaa
     2581 cgaatttcat tcttcatcga atattggcaa tgctgataat gtccgaaaaa acacctgctg
     2641 ccgtaacgct tgctcctgcc ccataacctt ttatcatcat gggaaattca ttataacgtt
     2701 ccgtagtcag cagaataatg ttgttgcttc cctgcaagtc gtaaaacgga tgggaattgt
     2761 cgatttcctg aagtccaacc tgacaatcac ctttatccat agaagccaca aaacgaaggc
     2821 gtttcccctg tttttcgagt tcctgccggc gaagttcaaa ggcttcatcc agttcgggaa
     2881 tttgttccca aaattgctcc aatgaacctt taaaaaactt ttccggtata aacagatttt
     2941 ttttcacatc atcctgttcc acctgataac cggcttcacg agccaggatc accaatttcc
     3001 tgaccacatc cttcccgctc agatcgatac gcggatcggg ttcggaatat ccatattcct
     3061 ttgccatcaa tacagcttta ctgaaaggaa tatcgctact caaagtatta aaaatgaaat
     3121 tcaacgttcc ggaaagaacg gcttctattt ttaagatatg atcgccgctg ttggtgaggt
     3181 tgttcatagt ggaaataatg ggtaatcctg caccgacatt ggtttcaaaa agaaatttta
     3241 tacctttctg acgagcagtt tcctttaatt tcatatactt gtcgtaagaa gatgaagtgg
     3301 caattttatt ggcagccaca accgaaatat tatgatcaag caaagtctgg tacaaatcgg
     3361 caatttgtgc attggcagta caatccacaa aaacggaatt aaaaatattc atattgataa
     3421 tttcgtcggc aatacgttca ggagtcgaat caacgccatg caatttcaat tcgtcctgat
     3481 aatgttcaat atcaattcct tcgcgcgaaa aaagcatttt tttgctggaa gcaattccaa
     3541 ccacattcag tttcaaacct ttctgatgca tcaacttagg ctgttgttgt tttatttgat
     3601 gcaaaagatg gctgcccacc ttgccaatac ccaccacaaa aatattcaac acctgatatt
     3661 cggacaggaa aaaggaatcg tgaatgacgt tcaaagcctt acgcaacgaa ctgcgatcgg
     3721 taacaaaaga aatattggtt tctgaagcac cttgtgcaca agccaccaca tttattccat
     3781 ttcgtcccag cgtaccaaaa agttttccgg caattccggg tgtacgtttc atattgtcac
     3841 caactatggc aacggtcgcc aagtcgtctt cagaaaaaat ttcatcaatt tctccaagtt
     3901 ccagttcatt ggcaaattct tttttcagta cttctactgc cagcgcacta tctgcatttt
     3961 ttaccccaat ggaagtcgta ttttcagaag aagcctgaga aacaaaaaac acactgatac
     4021 cgttttttgc cagtgttctg aaaattcggt aatttacacc aatgacgcct accattccca
     4081 atccacgaat ggtaatgagc gaagtatcgt taatggaaga aattccctta atggcttttg
     4141 atttttcgcc ggatttttcc tgagaaatat aggttccgct ggcttcgggg cgaaaggtat
     4201 tttttataac aatgggaata tttctgtgaa aagccgggaa aatggtaggc ggataaatca
     4261 cttttgcacc aaaattgctc aattccattg cttcgctgta actaagacgg tcgattacgt
     4321 aagcattgtt gataatacgt ggatcggcag tcataaatcc atcaacatcc gtccaaattt
     4381 ccagaattga agcattcaac gctgcagcaa gaatagctgc cgtataatcc gaaccgccac
     4441 gtcccaaatt ggttacctga ttggtatgta catcggtagc aataaatccg ccacaaacta
     4501 ctatagaagg agcatcctta aaaatctgct gaatgagttc attggtagtt ttaaaatcga
     4561 caatgtgttt atcgaactga ttttgagttt taataaatcg acgcgaatcg aaatgaacag
     4621 ctccctgaat tatattggca atcagaacgg aggaaatcat ttctccataa ctgacaaccg
     4681 tatcggtgat tttgacggaa gtatccttaa tcaaaaaaat tcccctgaaa atattcaaca
     4741 aatcggtaaa aatttcctgt atttgcagtt caacttccgc ctgcttttca ggcaacaccg
     4801 tttcggcaat catttgcatg tgcctggaaa acatttggtt atattcgtcg agataggttt
     4861 cgttgccgga agctgccagt tgggaaatac gataaagctg atcggtaacg cctgaaagcg
     4921 ccgaaacgac cactatcaca gattctttct gtgattcaac aatttttttt acattcaaaa
     4981 ccgcttcggt agaacctacg gatgttccac caaatttcat tactttcatt cgtcaggatt
     5041 ttattttttc ttcaaaaaat tttaagttgc aatattagtg attttttggc aaataaattg
     5101 caccttacca cgattttgag taaaagtatt gcatcttaat caaaattaaa gtacttttgc
     5161 atcgccttta acaaaaggaa tccggtcccg tagctcagct ggatagagca acagccttct
     5221 aagctgtggg tcgtgggttc gaatcccgcc gggatcacga aaaaagcagg taaaaataac
     5281 aataaattac ctgctttctt tttacaaaaa aaatcggcca tcatggcaaa gcaaactttt
     5341 tggagaataa attttctaaa aaaaccaaaa aacatgtttt gagacaacat catgctttgt
     5401 tcgtttcaca agattccatg aatggggatt tcacagtttt atcacaaaac ttttttacgg
     5461 caggtttaca tgaatgggca aaggagtttt atgtaaaatg aaaataatct cttatttttg
     5521 tatttatttt gtgagcgatt gaacttcaag aatgaaaaga aaaaaatttt tctctttttt
     5581 tcaaaaaatc cggtttaaat atcgtgtttc catattaaat gaaaacacac tcgaagaatc
     5641 gtggcatatt catttatcga ggttaagtgt ttttatatat ctttttgttt ttactataat
     5701 aacttttgcg ttgttgtctg tgttgatatt tgtaacacca ttgcatcaat atttgcccgg
     5761 ttatggagat atgaacttgc gatcaaaaat tattcaggaa tcaatccgag ccgattcttt
     5821 aactcatgca atgaatttgc aggcggcata tctggaaatt gttaaagata ttattaaggg
     5881 aaatattaaa cccgactctg ttgttggcgt tgattctgct tttttgcagc aacaagctca
     5941 aaaattaata gagacatcga aaagagaaaa agaatttaca aaaaaatttg aagaagaaga
     6001 aaagtttaat ttgacaagca tggaaagttc ttcgaccaat aagaaaacct acgtattttt
     6061 ccgtcctgca acaggtaata ttgtttcctc attcaatatt tccaaaaaac aatacggggt
     6121 agccttacaa actttacctg atgctgctgt gtcaagtgtt ttaaatggaa tcgtagtatt
     6181 tgcagatttt acttttgaca accaatgggt aatagcaatt caacatgaag acaattatct
     6241 ttcaatttat aagaacaaca atcgtttact aaaaaacata ggcgaggtag tgaggacagg
     6301 cgaaccgata gccatcaccg gttcgtccaa aggaaaaaac ggacaattgt atttcgagtt
     6361 gtggcaacaa ggtaaaccca ttaacccaca aaatatcata attttttaaa ctaccagatt
     6421 tttatttcta aaaacataaa caaattgaaa taaagtgtcc aacacaacca aacaaatagc
     6481 agttttaggc tccacaggat ctattgggaa gcaagccttg caagtgataa ccgattatcc
     6541 cgacaggttt agtgtttatg cactcacagc caataataat atagacttac ttataaaaca
     6601 ggctcgcaaa tttcaaccag aaatagctat tatagcaaac gagaaccatt ttctgcagct
     6661 gaaagaagct cttagcgacc ttcccataaa agtttttgct ggagaagaat ccattgcgca
     6721 gctggtagaa atgcaggcag tggatatcgt attaatagct ttggttggtt acgcagggct
     6781 aaaaccaacc atgaatgcca taaaggcagg caagaggatt gcattggcaa ataaagaaac
     6841 attggttgta gccggcgaac taatcgaaga aatggtggct tgctaccatc cttcaattat
     6901 acccgtcgac tctgaacatt cagctatttt tcaatgttta aatggggaag gggaaaaaac
     6961 ggaagttgaa aaaattatat tgactgcctc cggtggacct ttccgtacca aaaccagcaa
     7021 agagttggaa tcggtaacca ttgcagaagc cttgaaacat cctaattggg aaatgggaac
     7081 aaaaataact attgattcag cctccatgat gaataaaggt tttgaagtaa tggaagcaaa
     7141 atggttgttt ggtgttgcac cggagcaaat cgaagtggta gtacacccac aatccataat
     7201 acattcaatg gttcaattca ttgatggatc gataaaagct caattgggag ttcccgatat
     7261 gcgtttgccc atcttgtatg cttttacata ccctgaaaga attttaacca cttttccacg
     7321 tatagatttt aacctgttaa acagtttgac attcgaaaaa cccgatttgg agcggtttca
     7381 caatcttgct cttgcctatt atgctttaga caaaggaggg aatatgcctt gcgcattaaa
     7441 cgcagcaaac gaaatagtgg ttgatgcatt tttaaacagc aaacttagat ttttacaaat
     7501 gagcgaagtc attgaaacta ttttatcaaa aactttttat ataggtcatc cctcttatga
     7561 agattatgta caaacagata taataaccag acaaataacc agagagttaa ttgcacaagt
     7621 acattgaaac ttcagatgag agaaaaacaa aaatttaatt tataagtaaa cattttaata
     7681 atttagacga taaacaagaa aattcgcatg ttgaaattcg ttatcggatg aagttgaata
     7741 tttagatcaa tatggaaaca tttttaatta gagctttaca acttatactt agtttgtcaa
     7801 ttttagtagt actgcatgaa ttggggcatt ttgcctttgc ccgtttgttt aaagttcgtg
     7861 tggaaaagtt ttatattttc tttaatccgg gtttctctct attacgagca aaaaaaatta
     7921 atggcaaatg gcaatttaaa tttttttcga aaaatgtccc ctcgaatgag cgtcccaaaa
     7981 aagatgccga aggagatatc gtaatgggag aaaaagggaa acctcaaact gaacctatac
     8041 cgctttcaga gctttcagat gatgattgga gaaagtatcc cgaatcaact gaatggggaa
     8101 taggctggtt acctttaggg ggttactgca aaatttcggg tatgattgat gaatcaatgg
     8161 atacatcaca actaaaatcc gatccacagc catgggaata tcgttcacgt ccggtttggc
     8221 aacgaatgcc tattattaca ggcggagtat tggttaattt tatccttgca atggtaatat
     8281 attccgccat tctttttact tggggcaaag aatatctgcc tttggaaaat gctaaatacg
     8341 gcctgcattt tacatcggtc tttattgagc aaggatttaa aaatggagat aaaattttat
     8401 cggtcgacgg taaatcatgt gctgatttag gagaggtaat tgaaaaaata ctgattgaca
     8461 atgccaaaag tgttactgtt ctaagaggcg acagccaaat tttgactatt tcattgcccc
     8521 ccgattttcc tcaaaaagtt ttggcttcag gagaaagcaa tctgatttcg tatcgattcc
     8581 cattcgtggt acaagatgtt tcaccggaat ctcctgcaaa acaggcaaaa cttcaaccgg
     8641 gcgatagcat tgtaggtata aacggtaaag aattgacgat tttccaagat gtggtaagtg
     8701 ttttaaccaa ttatcgagac agtttaatac aacttgccta ttatcgaaat ggacaaaaag
     8761 aacaggcaac cgttcagctt gatcaaacag gtaaattggg tgtcgctgtt cgtcctttta
     8821 ccgaattttt ggaaaccgaa cacgtgaaat acggattgct ggcttccata cctgccggta
     8881 tttctctggg ttgggaaaca ttaaccagtt atataaaaca atttaaatta gtatttacca
     8941 aagaaggttc taaacaattg ggtggattcg ggtcaattgg gaaattgttt ccacaaacat
     9001 ggaattggca aattttttgg tctatgacag ccctgctttc agtcattctg gcttttatga
     9061 actttttgcc tattcctgct ttggatggag gctatgtgtt atttcttctg tatgaaatat
     9121 ttacggggaa aaagccaagt gacaaatttt tagagcaagc ccaaactata ggaatgcttc
     9181 tgttattggc tttgttgatt tatgccaacg gaaacgacat ttataaagcc atttttaaat
     9241 aaaaaaaata gaaaaatcag ggaggctgtc ttaaaagtag agacagtctc ttttttgtat
     9301 gtcgggcatg gtattaagct tgcaggatgc gtgtctctgt atgtgccgag ttgataagaa
     9361 acattagcga atggcaaggg tgttgagggt gactgcaaat ctgaaagaag ccatcagcaa
     9421 agctgacgtt ttgatgaaca gaaatctgat agaaggctct aatgagaggg taaccgagca
     9481 atggattggg aacgcccgaa attcaaccgt aactcaaatg gagtaaatca ggcaagacgc
     9541 agcggaagtt caatatctta tatcccgaga ggtcttgtag ggtacaccaa cagtgtaaga
     9601 aatgcaacga tgtattttag gccatataag aagtcagcag aggacatagt attctgttga
     9661 aaaacagaag aaggtctgaa tcttttaatt taaggagcag ttagttcaaa atttgtttta
     9721 tggagcagca gcaagcaata acgtaccaat acgacctgtt tgattcaaag ggtcaggatg
     9781 ttattcgccc tttcgcaggg agtgaagccg taagcggagc tcaggcgata aaggcgttgc
     9841 aagtaaaaga agcaggagaa cagaaacgag ccttaacgca ggatttgatg ggagggtggg
     9901 ggagtaatcc ccctacctac tcgatcatct tttcggacat aacttgtgaa agagtgtcaa
     9961 attgaagtaa ataatacaaa tgtcactttt ccaaaatcat cagatttttg ctccaagttg
    10021 tagaagaaaa aaattaaacg ttttttttca tgtcttcggc aaattctttc aagcgtttgt
    10081 caaggacagg aatttgatct ggtcggacaa atgaaacaca tatacgtaaa ccgtgattgt
    10141 ccttactacc tgtaatggag agtgaaatgg cactgagacc gtaataaaga aaattcttca
    10201 tcaactggct gctcgtcatc tccggataag ccactgtaaa ataaaaacca tctgccagct
    10261 cttcattttt ttctttgtaa acgatgtaaa atccattttt gagaaatatt tccttcatta
    10321 tttttgcttt ttcggcatat tctttcaaag cttccagaaa atttattttt ccatcattca
    10381 ctgcctttaa agttgctgca aatgcatatt gagctgtgtg tgaggtacct gccgtggtag
    10441 catagagcgc tccataagga ataaaatgtc ccaaaacgtc gctggtaaaa tagtttttaa
    10501 gttcaggata atgacgggta cgcaacgact cggaaataat catcatacca attcgctgtc
    10561 cggcataact gaagattttt gaagctgaaa gcaacaaaat gtagttttcg gtataacgag
    10621 ctacagtagg ctgataaggc ggcacaccgg gtttaccgta atcttttctg aaatccatgc
    10681 caaaataagc caaatcttcg acaacaataa cgtcatattc ggttgccaat tcgcctataa
    10741 tttgcaattc ttcttctgta aaattgatcc atgaaggatt attgggtgaa gaataagtaa
    10801 tggtgcaaat atctccttct tttaaaaatg aagttaatgc agaacgaagt ctgtcacccc
    10861 tattttccag aatgtcgaat gatttgaatg gaagtcccat gagttgcagt tgcgatttgt
    10921 ttaccggaaa tccgggatca ataaacaacg tacagggttt atttttgttg gtgcgggcag
    10981 cagccagcaa agcggcaaaa cttccctgca tggctcctac ggttggaatg cagttcaaag
    11041 gagtaacatc aatgtccatg aataattttg caaaacgtgc ggtttcgttt ttcacaaaag
    11101 gaattccttc aatatcggga taaagagcag ccaatcccct gtccaatgcc tccttttctg
    11161 catctacagt tgtttgcaaa ggaggaagac cgggcacacc catttccatg tggataaatt
    11221 ctacattggt tttttgttca atttgagcaa caagtttccc catttcacga atggagagtt
    11281 tatttatatc ttcctgtcca aattgtctga aaatctcatc caccaaatct ttagaaattg
    11341 acatgttttt taatttaaga ccattaaatt tttcgattat ctatcactct tacagctttt
    11401 ccttcacttc tcggaatact tttcggctca acaagcgtaa cttttacgct caaaccaagt
    11461 gcactgtgaa gcgaatgttc tatttttctg gaaagagaca tcaaatcctt tatggtatct
    11521 accattttag tttcgtccag ttccactttc agttcaagga tatcgagatt gttttctcta
    11581 tcaacatata acatgtaatg tgcacttgtt tcgcccattt cgagcagcac ctgttcgatt
    11641 tgcgatggga aaagatttac acctctgata atcagcatat catcacttct accagtaatt
    11701 tttttcattc ttaccaatgt acgtccacat tcacattttt ctcgattcaa tgaggtcaaa
    11761 tcgtgtgtat tgtagcgcaa caatggaatt ccttccttag tcaaagtggt gaaaaccaat
    11821 tccccctgtt ggccgttggg taaaggctct ttggttttag gatcgaccac ttcggggaaa
    11881 aagtgatctt catgaatatg taaaccatca tgaaactcgc aatcattggc aactcccggt
    11941 cccataattt cacttaatcc gtaaatgtca aaagctttaa ttccccactt tttctccaat
    12001 tcaagtctca tattctcaga aaacggctcc gctccaaata cacccgtttt aagtttcata
    12061 tcggagagcg aataaccaaa ttccgacaaa gcatcagcca aatgaagtgc atacgatgga
    12121 gtacaggcta acacagtgga accaaaatcg gacatgagtt gtaattgccg tcgtgtattg
    12181 cctgccgaaa tcgggacaac cgtacaacct acattttcgg caccgtaatg aattcccaat
    12241 ccgcccgtaa acagtccgta accaaatgaa acctgaacaa tatcttcagg cgtaactccc
    12301 gacatggtca gacatcgggc aagtacttcc tgccagtttt caatatcttt tttggtatat
    12361 ccaacagttg ttggtttgcc tgtagtaccg ctggaagcat gaatccgcac aatttccttc
    12421 atgggagcag caaacaatcc aaatggataa ttgtcgcgca aatcggtttt attggtaaaa
    12481 ggtaatttgg aaatatcatc aatagaacga atatcggaag gtctaacacc cttttcatcc
    12541 attttttttc gataaaattc cacattttga taaactttgt tgaccaattc tttcaatctt
    12601 tcactttgca gctttcgcat ttcactgcta ctcatgcatt cgatctcttc gttccaaatc
    12661 ataattgtgc ttttaaatcg gattcgtata tgggttttaa ttgatatttt tgaattattt
    12721 cgatggcttt ttcggtgttg tcgggacgca ttaccaatat ggatttggag ccaaaactga
    12781 aagcataaat atattcgatg gcaattccgt tgtcagaaaa atggttcaat acccgcgata
    12841 atgaacccgg aacaccttcc agctcgagcg ataaaacttc atgaatgcga actgtaaaat
    12901 tttcggtgcg aagggcttcg gctgcttttt cagcatcgga aaccagcatt ctaagaagtc
    12961 caaaatcaat gctatcggcc agcgtgaggg caataatgtt aatgttgttg cgtgccagaa
    13021 ccgctaaaat ctgattgaga tgtcctgttt tgttttctac aaaaacggaa agttgacgga
    13081 ttttcatggt tgtcagtata aaatgaatat ttatttaatt tcgtaattta actgcgcaaa
    13141 tttacaacaa attaataaat ttgttgggct tgtgttgtga ttttttaaaa aggtttatac
    13201 acgataaaag ctgaattcgt tcttgataat aaaaaaatct ataaatttgt cgaaaattta
    13261 tacaccaaaa aattttatca tgttcccata tccattactt cataggaagg aattaattcg
    13321 gcaaatgaat aatttaggaa aatccaacac tccatttttt ttcctactgg attttatggc
    13381 gcaaaatgga tatattatca aaaatcaaga tctggacgac caatatatac gatttcaatt
    13441 gtcggctgaa agtttttcca atcaaaaaaa atcgacaaaa aattcattta attttcaatg
    13501 gaatattcat cccgtaaatt ttgatgaata ccaattgaaa tttcattttg taatggaaaa
    13561 tatccgatgc ggaaattctt ttttactgaa tttaacgcag ccaacagaag taagtaccaa
    13621 cttaaatttg cttgaattgt atgagttaag tgtttcaaaa tacaaactat ggctcaaaga
    13681 tttatttacc gttttttcgc ctgaaacttt tataaaaatt gaggatggaa ttatttccac
    13741 ttttcccatg aaaggaacta ttgatgcttc tgttgcaaat gccgaagatg tgattttgaa
    13801 cgacttcaaa gaacaagctg aacatgcaac tattgtagat cttatgcgta atgatttaag
    13861 tatggttgcc gaaaaagttg aagtcaagaa atacagatac atcgataaaa taattaccaa
    13921 tcaaaatgaa ctatttcaaa cgagttcaga aattacagga gaattaccag aaaattattg
    13981 ttgttatttg ggaaatatta ttatttccat gcttcctgcc ggttcgattt cgggtgctcc
    14041 aaaacgtaaa acattggaaa tcatcagaga aattgaagga tatgacagag gtttttatac
    14101 cggaattttc gggcaatatg atggaaaaaa tttggattca gcggtactga tacgcttcat
    14161 tgagcaaaag aacggacaaa tgattttcaa aagcggagga ggaataacat cgaacagcga
    14221 agttttaaaa gaatatcgag aaatgttaga aaaagtgtat gtaccgattt attgaatcca
    14281 taaaactgtt ggatggaatt ttttatcgcc tcaaatttca tcagaagaga atgaatattt
    14341 gttcggaatt attttatccg aatgacaatt ttattcatct ccaatcggct ttaaccgatt
    14401 cacatccacc acaaaagggt ttattcaaat gcaaaattac gtttgaatct aaaattttga
    14461 agatagaata tttaccatac aaaaaaaagg aaattaattc tcttcgcata gtacataccg
    14521 agatagaatc acgtccctat aaaaaggaag atcgtacaga ttttaacgca gcatttgaca
    14581 tgagaggaaa ttatgatgac gttttgttga tgaaaaacgg cttattgaca gatacttcta
    14641 tttgcaatat tgcccttttt gacggttttt cttggcaaac gccaaccaaa ccattgattt
    14701 atggagttaa tagagctaat ttgttagaag aaggaaagat tttcgaaaaa gatattaaac
    14761 ttagcgattt gcaaaattat tctgccatta ggctttttaa tgctttgatc gaatttggag
    14821 aaattgaatt gaatatcagc aatatacact tttaaaaaag aaaaattgta aattttttta
    14881 gtgctaaaat tgagaattga aacaaaacaa cagaaaaaaa tttcacttta tattttgcat
    14941 ttagaaaaat tgcagtatct ttgcgaagaa tttacaaatt aaaacaaaac atgaaactga
    15001 attctctaca tactttgcta agcagcatta ttctactttt ggcaaaagag cagaagtgag
    15061 tttggtatgt agatatgata accgatttta tttgaaaaac ctttctcact taatgtaaga
    15121 aaggtttttt actttattat attaatgata aaattaacca actttgagtt acaattcaaa
    15181 aaacaaaaaa atatggacag attatttatt tttgacacga ctttacgtga cggcgaacaa
    15241 gttccaggtt gccaactcaa tactgtcgaa aaaattgaag tagcaaaagc gcttgaaaat
    15301 ttaggtgtcg atattataga agcgggcttt cccgtttcga gtcccggcga tttcaattcg
    15361 gttgtagaaa tttcaaaaac tgttacttgg ccggttattt gtgccctgac ccgcgccgta
    15421 gaaaaagaca ttgaagtggc agcagaagca cttcaatatg ccaagaagaa aagaattcac
    15481 accggcatag gaacttcaga atatcatatc cgttacaagt tcaattccaa tcaggaagaa
    15541 attctggaac gctcggtagc agcagtaaaa tatgcaaaaa aatttgttga agacgtggaa
    15601 ttttattgtg aagatgccgg tagaacagaa aatgagtatc ttgcacgcgt ggtagaagcc
    15661 gtaatcaaag ccggagctac tgttgtcaat attcccgaca ccacaggata ttgcctccct
    15721 tgggaatatg gtgaaaaaat caaatatcta atggaacatg tggatggtat tcacaaagcc
    15781 ataatttcta cacattgtca taacgatttg ggtatggcaa ctgccaacac catatcggga
    15841 atcatgaatg gagcacgtca agccgaagtt accataaacg gtatcggaga acgtgcagga
    15901 aacacagctt tggaagaaat tgccatgatt ttgaaatgtc ataaaaaact gaatatcgaa
    15961 acacaaatca ataccaaaaa gatttattca accagccgtc tggtttccaa tttaatgaat
    16021 atgcccgttc agcccaataa agcagtagtt ggtagaaatg cttttgctca ttcttcggga
    16081 attcaccaag atggcgtatt gaaaaacagg gagaattacg aaattatgaa tccagaagac
    16141 gtaggtattg atcacaattc aatagtgttg acagcacgga gcggacgtgc cgccttgaaa
    16201 cacagactga atactttggg cgtggaattg gcagataaaa aactggatgc tgtttatgaa
    16261 gaatttctga agttggccga caagaaaaaa gatataaacg acgatgattt gctgatgctt
    16321 gccggtaaag aaaggtcaga gaaagaacgt ataaaactcg actatttaca ggtaacttcc
    16381 ggtgtggggg taaaaccggt agcaagtatc ggactcatta tcggcagtga aaaatttgaa
    16441 gccgcttc
//
LOCUS       DAOSCH010000045        15379 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2632_length_15379_cov_6.166471, whole genome shotgun sequence.
ACCESSION   DAOSCH010000045 DAOSCH010000000
VERSION     DAOSCH010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15379
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2632_length_15379_cov_6.166471"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..587
                     /locus_tag="PLZ54_04950"
     CDS             72..587
                     /locus_tag="PLZ54_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66311.1"
                     /translation="MFTTIEISAQEIGNLPEKTPEQEAMQQTSKLQQELDLNAEQVKK
                     IYEINLRYAKQRQSVTKRSEVMELLKRKDGEIENALGKEKYQQLQNKRNERISIDVPT
                     TSGMQNITPSYRSTKRTGNTNLSFPERNLQNDSKTSDGNTEQPKRLTPNNSRRTTDDA
                     ERKTTSPQQRK"
     gene            594..1307
                     /locus_tag="PLZ54_04955"
     CDS             594..1307
                     /locus_tag="PLZ54_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOJ66312.1"
                     /translation="MSHPFFQFKQFTVFHDRCAMKVGIDGVTLGAWTPVDNAKKILDI
                     GTGTGLIALMLAQRSTAEIDALDIDKQAIEQARFNVENSAWKNRIRLFETSLQEYHSV
                     CTTTYDVLVCNPPYFINSKKSPIVARNMARHAESLTHEELIDLSFDLLKPDGTLCVIL
                     PVEEGLQAISHALASNWYAHQMVFVFPKPAAKAKRLLIQLGKKEKITRISRLAIETEQ
                     RHEYTDEYKSLVKNFYLKL"
     gene            complement(1328..1642)
                     /gene="trxA"
                     /locus_tag="PLZ54_04960"
     CDS             complement(1328..1642)
                     /gene="trxA"
                     /locus_tag="PLZ54_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOJ66313.1"
                     /translation="MVIEFTDENLNEFIESGKPVLVDFWAEWCGPCRMIGPIVEELAE
                     EYDGKVQVGKLNVDENAEVPTKFGIRNIPTLLFFKDGQLVDKQVGATSKSVLAEKIKS
                     LI"
     gene            complement(1766..5557)
                     /gene="dnaE"
                     /locus_tag="PLZ54_04965"
     CDS             complement(1766..5557)
                     /gene="dnaE"
                     /locus_tag="PLZ54_04965"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOJ66314.1"
                     /translation="MNTFVHLHVHSHYSILDGMSSIDQLVDKAMKTGMPAIALTDHGS
                     MFGIKEFYNYVKKKNVPVYENISLLQKQLNKDELSDIQKDEIHKNIETEKRKLFKPII
                     GCETYVAHRSRFSKESQEDRSGYHLILLAKNKTGYRNLCKLISLAWIEGMYYRPRIDH
                     EILEKYSEGLIASSACLGGEIHKKIESGNLEEAEKAVLWYKKVFGDDFYLELQRHKTD
                     KPGGDTSTYEKQTLQNVELIKLARKTNTKLIATNDVHFVEEEHAEGHERLICLSTGKD
                     MDDPTRLRYTKQEWLKTPQQMQEIFSDIPEALENTLEIAQKVEFYDIDSAPMMPTFKI
                     PEEFADENQYRSRYTEAMLREEFGNGFDRLGGMDKVIRIKLEADYLRNLTFEGAKERY
                     GENLDPELEERINFELNVIKTMGFPGYFLIVQDFIAAARNMGVAVGPGRGSAAGSVVA
                     YCLKITDIDPLKYDLLFERFLNPDRISMPDIDVDFDDDGRNEVLRWVTEKYGKDRVAH
                     IITFGTMAAKSAIKDVARVQKLPLSEAERLTKLIPDKLPDTPDGKKMKVNLSNCFKFV
                     PELEIARNSSDENLSSTLRYAEMLEGTVRQTGVHACGIIIGADDLTKFVPLSTAEDKD
                     TKEEILVTQYEGSVIEEVGLIKMDFLGLKTLSIIKDTLENIRKSKNIEVDLDKIPLDD
                     PETYQLFSKGQTVGTFQFESAGMQKHLQALQPTQIEDLIAMNALYRPGPMQYIPQYIN
                     RKHGLEKIEYPFPIMEKRLKETYGITIYQEQVMLLSVDLAGFTRAEADELRKAMGKKL
                     TGKMQALKEKFIAGCQKNGYGPLEKLEQIWSDWVEFSNYAFNKSHATCYSYLAYRTAY
                     LKAHFPSEFMAANLSRNKDDIDEVSKFMDECRNMGINVLGPDVNESDFTFSVNEKNDI
                     RFGLGGIKGVGENAVMAIVNERHAHGKFKNIFDFVERVNLTACNKRAIESLAIAGAFD
                     GLNDIKREQFFVENGKGETVIESLIRYGNKYQADRLMSQNTLFGDMNSIEINKPEIPY
                     APAWAPLNRLNRERELIGMFLSAHPLDQFEFEMNYICNTSTGQLKDLTSLKGKQLKIG
                     GLVTNIRHSVSKAGKPYGVFTLEDYDGSYEFVLYGNNYIEFQKYMIKDMFVFIQAIVQ
                     EKYADSKYRKNTDIGGEQELELKIQKIDFFSDIKDKFVNNLKINIPLQQLTEDTVEML
                     SKIILGNKGNVNVYIQVQDQNSFNKINFFARQHRVDINPKVYRMLKTAKSEGILDFDV
                     N"
     gene            complement(5776..7035)
                     /gene="nqrF"
                     /locus_tag="PLZ54_04970"
     CDS             complement(5776..7035)
                     /gene="nqrF"
                     /locus_tag="PLZ54_04970"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004333674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HOJ66315.1"
                     /translation="MLLAIQTSTLIASLLVFFVVIFLLVVVLLVAKQYLVASGNVTIT
                     INNEKKLEVPAGGTLLSTLVNQQIYLPSACGGKGTCAQCRCQVLEGGGEILPTETGHF
                     SRKEIKEHWRLGCQVKVKNDLSIKIPESILSIKKWECEVISNKNVATFIKELIVKLPE
                     GEHMNFLPGSYIQIDIPPYELKFSEFEVEERFRPDWDKMHLWDLTCSNDEETMRAYSM
                     ANYPAEGDIIMLNVRIATPPIDRATGKFLDVKPGIASSYIFRLKPGDKVNVSGPFGEF
                     HPIIDSKREMLYIGGGAGMAPLRSHILHLLKTLRITDRKISYWYGARSKNEIFYQEDF
                     RELEKEFPNFSFHIALSEPLPEDNWTGPVGFIHSVILNEYLKFHEEPEEIEYYMCGPG
                     PMAKAVEKMLDDLGVPREMLMFDDFGA"
     gene            complement(7144..7761)
                     /gene="nqrE"
                     /locus_tag="PLZ54_04975"
     CDS             complement(7144..7761)
                     /gene="nqrE"
                     /locus_tag="PLZ54_04975"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HOJ66316.1"
                     /translation="MESILNIFVKSIFVDNMIFAYFLGMCSYLAVSKNVKTSLGLGTA
                     VTFVLFITVPVNYLLQNYVLKEGALQWLSPQLASLDLSYLSFILFIAVIAAITQIVEM
                     AVEKFSPSLYSSLGIFLPLIAVNCSIMGASLFMQQRAFHNIGEATSYALGSGVGWLLA
                     IVGLAAIREKMEYSNVPKPLRGLGITFITVGLMGIAFMCFSGLKI"
     gene            complement(7833..8465)
                     /locus_tag="PLZ54_04980"
     CDS             complement(7833..8465)
                     /locus_tag="PLZ54_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HOJ66317.1"
                     /translation="MSTLLSKKSRQVLLGPLSKNNPITIQVLGICSALAVTAKLEPAL
                     VMGISVTIIVAFSNIIVSLVRNTIPNRIRIIVQLVVVAALVTIVSEILKAFAYDVSVQ
                     LSVYVGLIITNCILMGRLEAFAMSNSPWESFLDGLGNGFGYAIILIIVSFFRELFGSG
                     TLLGYNIIPQSFFNVGYMNNGLMMMPPMALILVACIIWVHRAKNKELQEK"
     gene            complement(8462..9187)
                     /gene="nqrC"
                     /locus_tag="PLZ54_04985"
     CDS             complement(8462..9187)
                     /gene="nqrC"
                     /locus_tag="PLZ54_04985"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HOJ66318.1"
                     /translation="MNTNSNTYTLVYATVMVVIVALMLAFVSGALKEKQNSNIELDKK
                     KQILKSLRIDAKQNNIAQLYDQYVIKGLIVNTNGEILSYSKKEAFDIDIAKELSKKVE
                     ERRLPIFIAQVEGETKYIISLRGTGLWGPIWGYVSLNDDKNTIYGAYFSHASETPGLG
                     ANIAEPNFQRQFEGKHIFNEKNEFVSIAVMKVGKKAEGREQVDAISGGTITSKGVETM
                     LLNCIGQYEAFFRQLSTIGEIKQ"
     gene            complement(9235..10443)
                     /locus_tag="PLZ54_04990"
     CDS             complement(9235..10443)
                     /locus_tag="PLZ54_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HOJ66319.1"
                     /translation="MNALRNFMDKIKPYFEKGGKLEKLHSVYDGFETFLFVPTTTAPK
                     YGSHIHDPVDTKRTMSTVIVALVPALLFGMYNVGYQHYLAIGQDVNFWTEFLFGFLAV
                     LPYIVVSYAVGLGIEFIGAQIKGEEIQEGFLVTGFLIPLIIPINTPLWMVAVATAFAV
                     IFAKEVFGGTGMNIFNVALVARAFLFFGYPTHMSGDSVWIRTGTALGFGGGNVVDAFS
                     GATPLGQIATASTSVPHITDTFGHSLGFWDGFVGLIPGSVGETSIIAIMLGAFVLLIT
                     EVASWKTMLSVFFGGALMGIVFNLFGPDTAAAHFPWYNHLVFGGFAFGAVFMATDPVT
                     SARTEKGKWIYGLLIGCLAIIIRVLNPGYAEGMMLAILLMNIFAPLIDFYVVDANVKK
                     RISRAQKSIQ"
     gene            complement(10502..11884)
                     /locus_tag="PLZ54_04995"
     CDS             complement(10502..11884)
                     /locus_tag="PLZ54_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HOJ66320.1"
                     /translation="MTNVIKTTKGLDIPIDGAAQKMIERTVISDIVALVPDYYHGIIP
                     KVLVRAGDRVKAGTPVFHDKIHENLNIVSPVSGKVLAVNRGEKRKLINITIERDVVNE
                     YENFGHPSLESLSGEEIKSALLKAGIWPYIKQRPYDVIANPNKTPKSIFISSFDTAPL
                     APDYNFILKGQFADFQTGIDALAKLTPGKVHLGVKANVPSNEFLKVKNVEITEYEGPH
                     PIGNVGIQINHVDPINKGEVVWTVNVQDMLYIGRFFNKGIIDLKKIIALTGPEVIEPK
                     YYTTLPGTAVAPFINGNVHKGIPLRYILGNVISGIKVDINDFIDPYASQIFVIDEGTE
                     IHEFLGWAMPRLNKFSATKLFFTPLLQKKFFKKIFGDIKFSYDARILGGRRAIIMSNE
                     YDRVLPMDILPEYLFKAMITGNIDKMEALGGYEIAPEDVALCEFVCTSKLPLQLIVRE
                     ALDMMKKELE"
     gene            12379..14157
                     /locus_tag="PLZ54_05000"
     CDS             12379..14157
                     /locus_tag="PLZ54_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOJ66321.1"
                     /translation="MDWKEIYLQAINWREKHIKERQFILILSFLTGIFAALAAFVLKM
                     AIHSVQSLVTESFNRESLNYLYLITPAVGIIITSLFVRHIVKDDISHGVTRILYAISQ
                     RKSILKLHNVWTSLVGSSITIGFGGSVGAEAPIVLTGSAIGSNLGKFFKMNQKTMMLL
                     IGCGAAGAIAGIFKAPIAGLVFTLEVLMLDMTMTSIVPLLISSVTATSVTYILTGDEF
                     MFQFTNYEPFAVERIPYLLMLGVVCGLVSLYFTRGMNWLENFYKKFENPYIKWAIGGS
                     ILSVLIFLFPPLYGEGYDTIASLLNNDISSVVKQSFFLGSENKPLVLLTYLVLIIVFK
                     IFATASTTSAGGVGGIFAPSLFVGCITGFVVAEILNLIGLNMPIENFALAGMAGLMSG
                     VMHAPLTGIFLIAELTGSYYLFMTLIIVSTISYLTIILFEPHSLYAMRLAQKGELITH
                     NKDKAVLTLLKMENVIETNLNVLEPDMKLGEVIKIISHSKRNVFPVVNPKNGVLLGIL
                     TLDEIRNIMFRPELYQRFTVRQLMISPPTFLNVNMPMEKVMQIFEDTGAWNLPVVDDE
                     KHYIGFVSKSTIFNSYRHVLVHFSDE"
     gene            14201..14404
                     /gene="xseB"
                     /locus_tag="PLZ54_05005"
     CDS             14201..14404
                     /gene="xseB"
                     /locus_tag="PLZ54_05005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOJ66322.1"
                     /translation="MTKNKPTYDEAIAELDKILAELENNSDINMDEIAQKVKRASELL
                     QICKKQLHVLDENLEKLLSELEE"
     gene            complement(14405..15064)
                     /locus_tag="PLZ54_05010"
     CDS             complement(14405..15064)
                     /locus_tag="PLZ54_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HOJ66323.1"
                     /translation="MQIYISELIRQACPDLTLSVIACEVKNSETTSILWQEIEEEMEK
                     ISSTFTLENIKMRPEISATRQVYRKLGKDPNRYRPSAEALCRRICKGVGIQRVSTLVD
                     IINLISIRSGFSIGGFDAKKIQGDLRLEVGTANDIFQAIGRGILNVEGLPLFKDAAGG
                     IGTPTSDNERTKLTMETRRLLMIINGYSGEKGLQEASEQARMLLEKYAEAKEVNITLV
                     K"
     gene            complement(15069..>15379)
                     /locus_tag="PLZ54_05015"
     CDS             complement(15069..>15379)
                     /locus_tag="PLZ54_05015"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HOJ66324.1"
                     /translation="EDIFVVAGPSISPSVYEVGRDVVEEFQKNDWNLSDIFSQNDGKI
                     FLNLWKANEISLLGSGLQKNNIEFLCRCTFTESENFFSARRQGILCGRMLSGIMLKQA
                     "
BASE COUNT         4609 a         3056 c         2863 g         4851 t
ORIGIN      
        1 aattaattct gtttttttga aaggtggata tgaaaatcaa atttatttta catatatttt
       61 tctgttcttg tttgtttaca acaattgaaa taagtgctca ggaaataggg aacttaccag
      121 agaaaacgcc tgaacaggaa gccatgcagc aaaccagtaa actacaacag gaattggatt
      181 taaacgcaga gcaggttaaa aagatttacg aaatcaacct tcggtatgcc aaacagcgtc
      241 agtcggttac aaaacgttcg gaagtcatgg aactgctgaa aaggaaagat ggtgaaatag
      301 aaaatgcgct gggcaaagaa aaatatcagc agctccagaa caaacgcaac gaacgaattt
      361 ccattgatgt cccaactaca tcgggtatgc aaaatattac gccttcctat cgttccacca
      421 aaagaaccgg aaatacgaat ttgagttttc ccgaaagaaa tttgcaaaac gacagcaaaa
      481 cttcagatgg taatacggaa caacccaaac gtttgactcc caataattcg aggcgtacca
      541 ctgatgatgc ggaaagaaaa accacttcgc ctcaacaaag aaagtaaaga tacatgtcgc
      601 acccattctt tcagttcaaa caattcactg tttttcatga tcgttgtgcc atgaaagtgg
      661 gaattgatgg cgtaacgctt ggagcttgga caccggtaga taatgcaaaa aaaattcttg
      721 atatcgggac gggcaccggc ttaatagctt tgatgctggc tcaacgaagt acagctgaaa
      781 ttgacgcgtt ggacattgat aaacaagcta ttgaacaagc ccgtttcaac gtggaaaatt
      841 ccgcatggaa aaatcgcatt cgtttgtttg aaacttctct gcaggagtat cattcagttt
      901 gtacaacgac ttacgatgta ttggtgtgta atccacccta tttcataaat tccaaaaaaa
      961 gtccgattgt tgccagaaat atggctcgtc atgcagaatc tcttacacat gaagagctga
     1021 ttgatctttc ctttgatttg ttgaaaccgg acgggacatt gtgtgttatt ctgccggtgg
     1081 aggaaggttt gcaggcaatt tcacatgctt tggccagcaa ttggtatgct catcaaatgg
     1141 ttttcgtttt tcccaagcct gcagccaaag ccaagcgatt gcttattcag ttgggtaaaa
     1201 aagaaaaaat cacccgtatt tcgcgacttg ccatcgaaac ggagcaacgc cacgaatata
     1261 cggatgaata caaaagtctg gtgaaaaatt tttatttgaa actgtagtag ttaaatcaga
     1321 gaaaaaatta aatcaaagat ttgatttttt cggccaagac tgatttggaa gttgcaccaa
     1381 cttgtttatc gacgagttgt ccatctttaa aaaacaataa ggttgggata ttgcgaatgc
     1441 caaattttgt tggaacttca gcgttttcat ctacgtttaa ttttccgact tgcacttttc
     1501 cgtcatattc ttcggccagt tcttctacaa taggacctat cattctacaa gggccacacc
     1561 attctgccca gaaatcaacc aatacaggtt ttccactttc aataaattcg ttaagatttt
     1621 cgtctgtaaa ttcaatgacc ataatttttt aagttttgaa gtttgcaata attttttatt
     1681 caaaaaacaa atatcggtaa caaaagtttt ataattaaac actattttgc tatagaaaaa
     1741 atcaattaaa aattacattg acttatcaat ttacatcaaa atcgagtatt ccttcacttt
     1801 tggcagtttt cagcattcga tatactttgg ggttgatatc cacgcgatgc tgtcgggcaa
     1861 aaaagttgat tttattgaaa gaattttgat cttgtacctg aatatataca tttacatttc
     1921 ccttatttcc aagaataatt ttacttaaca tttcaacggt atcttccgta agttgctgca
     1981 gtgggatatt aattttcagg ttattgacga atttgtcttt aatatctgag aaaaaatcga
     2041 ttttttgtat tttaagttca agttcttgtt ctccgccaat atctgtattt ttcctgtatt
     2101 tgctgtcggc atatttttcc tgaacgatgg cctgaatgaa aacaaacatg tcttttatca
     2161 tgtatttctg gaattctata taattgttgc catacaacac aaattcatag gaaccatcgt
     2221 aatcttcgag agtgaatacg ccgtagggtt ttcctgcttt ggacacggaa tgacggatat
     2281 tggtgacgag tccgccaatt ttcagttgtt ttccttttag cgaagtcaaa tctttcaatt
     2341 gtccggtaga agtgttgcag atgtaattca tttcaaattc aaactgatcg agtgggtgag
     2401 cagaaaggaa catgccgatc aattctcttt ccctgtttaa tcggttaaga ggagcccacg
     2461 ccggcgcata gggaatttcc ggcttgttga tttcaatgga attcatatcg ccaaacagtg
     2521 tattttgcga cataagtctg tcggcttgat atttgttccc gtaacgaatc aagctttcga
     2581 tcaccgtttc tcctttgccg ttttccacaa aaaactgttc gcgtttgatg tcgtttagtc
     2641 cgtcaaaagc tccggctatt gccagacttt caatagcacg tttgttgcaa gccgtaagat
     2701 tgacgcgttc cacaaaatcg aaaatgtttt taaattttcc gtgtgcatgt cgttcgttga
     2761 ctatggccat gacggcattt tcgcccacac ctttgatacc gcccaaacca aaacgaatat
     2821 cgtttttctc gttgaccgaa aaagtaaaat cactctcatt gacatcggga cccagcacat
     2881 tgatgcccat atttcggcat tcgtccataa atttgctcac ttcgtcgatg tcatccttat
     2941 ttcttgagag gttagctgcc atgaattcgg acggaaaatg agctttcagg taagcggtac
     3001 gataagcgag ataagagtag caggtggcat gcgacttatt gaaagcataa ttggaaaatt
     3061 ccacccagtc gctccaaatt tgttccaatt tttcgagtgg cccatagcca tttttttggc
     3121 aacctgcaat aaatttttcc ttcaaagcct gcattttgcc ggtgagtttt ttccccattg
     3181 cttttcggag ttcatcagct tcagcacgtg taaagccagc caaatcgaca ctcagcaaca
     3241 ttacctgttc ctgataaatg gtaataccgt aagtttcttt cagcctcttt tccatgatgg
     3301 gaaacggata ttcaatcttt tccaatccat gttttcggtt gatatactga ggaatatact
     3361 gcattgggcc gggacggtaa agcgcattca tggcaattaa atcttcaatt tgtgtgggct
     3421 gaagagcttg caaatgtttt tgcatgccgg cagattcaaa ttgaaaagta cctacagttt
     3481 ggcctttgct gaaaagttga taggtttcag gatcatccaa tggtattttg tcgagatcta
     3541 cttcgatatt tttggatttt ctgatgtttt ccaatgtatc tttgataatg gaaagcgttt
     3601 tgagacctaa aaagtccatt tttatcaatc cgacttcctc tatgaccgaa ccttcgtatt
     3661 gcgtaaccag aatttcttct tttgtgtcct tgtcttcggc agtggataat ggaacaaatt
     3721 ttgtcaggtc atcggcacca atgattatgc cacaggcatg aactccggtt tgtcgtacag
     3781 tgccttcgag catttcggca tagcgaagtg tgctggaaag attttcgtcg gaagaatttc
     3841 gggcaatctc caattcggga acaaatttaa aacaattgga caaattgact ttcatttttt
     3901 ttccgtccgg cgtatcgggc aatttgtcgg gaataagttt ggtcaatctt tctgcttcgg
     3961 aaagtggcaa tttttgaacc cgcgccacat cttttatggc tgattttgct gccatagtgc
     4021 cgaaagtgat aatatgggca actctgtcct ttccgtattt ttcggtaacc cagcgtaaaa
     4081 cttcatttcg tccgtcgtca tcgaaatcga catcaatatc gggcatggaa atacggtcgg
     4141 gattcagaaa acgctcgaaa agcaaatcat atttcaaagg atcgatatcg gtaattttca
     4201 ggcagtaagc tacaaccgaa ccagctgccg aaccacgacc cgggccaacg gcaacaccca
     4261 tatttcttgc cgcagcaata aaatcctgaa caataagaaa ataaccggga aatcccatag
     4321 ttttgataac gttcaactca aaattgatgc gttcttcaag ttcgggatcc agattttctc
     4381 catagcgttc tttagcacct tcaaaagtaa ggtttctcaa ataatcggct tccagcttga
     4441 ttcggataac tttgtccatg ccacccaaac ggtcaaatcc attaccaaat tcttcacgca
     4501 gcatggcttc ggtgtaacgt gagcggtatt gattttcatc ggcaaattct tccggaattt
     4561 tgaaggttgg catcatgggc gccgaatcaa tatcgtaaaa ctctactttt tgagctattt
     4621 cgagtgtatt ttcgagcgct tcaggtatgt ccgaaaatat ttcctgcatt tgttgaggcg
     4681 ttttcaacca ttcctgtttg gtgtaacgca ggcgtgtggg atcgtccata tcttttcccg
     4741 tactgagaca aatcaatcgt tcatgtcctt cggcatgttc ttcttccaca aagtggacat
     4801 cgttggtggc aatcagtttt gtattggttt ttctggccag ctttatcaat tcaacatttt
     4861 gaagggtttg tttttcgtag gtagaagtgt cgcctccggg tttgtcggtt ttatgacgtt
     4921 gcagttcgag atagaaatcg tcgccaaata cttttttgta ccaaagaaca gctttttctg
     4981 cttcttccaa gttgcccgat tcaatttttt tgtgaatttc acctcccaga caagctgaag
     5041 aagcaattaa accttcactg tatttttcca gaatttcatg gtcgatacgc ggtcgataat
     5101 acattccttc tatccaagcc agcgaaatga gtttgcatag atttcgataa cctgttttat
     5161 ttttggccaa caaaatgaga tgataaccac tccggtcttc ctgactttct ttcgagaaac
     5221 ggcttctatg ggctacataa gtttcacatc caatgattgg tttgaaaagt ttacgttttt
     5281 ctgtttcaat atttttatga atctcatctt tttgaatgtc agataattca tctttattta
     5341 attgcttttg caaaagggaa atattttcat aaacaggaac atttttcttt tttacgtagt
     5401 tgtaaaattc ttttattccg aacatgctgc cgtgatcggt gagtgcaata gccggcatgc
     5461 cggttttcat agctttgtcg accaattgat ctatggaaga catgccgtcg agaatagaat
     5521 aatgcgagtg aacgtgcaaa tgaacaaacg tattcatgct aaataggtga agtattgaat
     5581 attagaaaag ctgaaaaact ttaaatttag gtgtttaaag ttagtaaaat ttttgttgtg
     5641 gacagattta tatcgaagga aaaatttcaa aaaaaggaaa taaaatttat tctcttttga
     5701 ttttcaacag tgtctgaaaa ataaatcagg ggctgtatgt tgataagccc ctgaaaaaga
     5761 aatattaaag aattttcaag ctccaaaatc gtcaaacatc agcatttcac gcgggacgcc
     5821 gagatcgtca agcatttttt cgactgcttt ggccattgga cccggcccgc acatataata
     5881 ttcaatttct tctggttctt cgtggaattt gagatattca ttgagaataa cgctatgaat
     5941 aaaccctaca ggaccagtcc agttatcttc gggaagaggt tcacttaaag ctatgtggaa
     6001 cgagaaattg ggaaattctt tttccagttc acggaaatct tcttggtaga aaatttcgtt
     6061 tttcgaacgt gcgccatacc agtaggaaat ttttcggtcg gttatgcgta aggttttcag
     6121 caaatgcaag atatgggaac gcaagggagc cattccggcg cctccaccga tgtaaagcat
     6181 ctcgcgtttt gaatcgataa ttgggtgaaa ctcgccaaac gggccggaaa cattcacttt
     6241 atcgcccggt ttaagacgga aaatataaga agaagcaata cccggtttta catctaaaaa
     6301 ttttcctgta gctcggtcaa tgggaggggt ggcaatacga acattcagca tgatgatatc
     6361 gccttcggcc ggataattgg ccattgaata ggcacgcatg gtttcttcgt cattgctgca
     6421 tgttaaatcc caaaggtgca ttttatccca atcgggacgg aaacgttctt ccacttcaaa
     6481 ttcactgaat ttgagttcgt atggtggaat atcaatttga atgtagcttc cgggcagaaa
     6541 attcatatgc tctccctcag gaagttttac aatcagttct ttgataaagg tcgcaacatt
     6601 tttattggaa atcacttcac attcccattt tttaatgctg agaatggatt cgggaatttt
     6661 tattgaaagg tcatttttta ccttcacctg acaacccaat cgccaatgtt ctttgatttc
     6721 ttttcgggaa aaatgtcctg tttcggtggg taaaatttcg cctccgcctt cgagtacctg
     6781 acagcgacat tgagcacaag ttccttttcc gccacaggca gagggtagat atatctgctg
     6841 attgaccagt gtactcaaca aagttcctcc ggccggcact tccagttttt tttcattatt
     6901 aatggtgata gtaacgtttc ccgaagcgac aagatattgt ttggcaacca ataaaaccac
     6961 taccaataaa aatataacta caaaaaatac caataagctg gcaatcagtg ttgaagtttg
     7021 aattgctaat aacataattt aattgacgtt atccttcccg ttttttttga aggaataatt
     7081 tattgattaa ttaataccta ttgcaaataa aatgtataaa ataaaaaaac agaagatttt
     7141 aaattaaatt ttcaagcccg agaaacacat aaaggcaatc cccattaaac ctacagttat
     7201 aaaagttatt cctaaaccac gtaacggttt cggaacattg gagtattcca ttttttcacg
     7261 aatggctgcc agtccgacaa tagccagcaa ccagccgacg ccggaaccca aagcgtagga
     7321 tgtagcttcg ccaatattgt gaaaagctct ttgctgcata aataacgatg ctcccattat
     7381 ggaacaattt actgcaataa gtggcagaaa aattcccaat gagctgtaga gtgaaggaga
     7441 aaatttttca actgccattt caacaatttg tgttatggcc gctatgacgg caataaacaa
     7501 aatgaaagag agataactca aatcgagtga agccaattgt ggactaagcc actgcaaagc
     7561 tccctctttt aaaacgtaat tttggagtaa ataatttacg ggaacggtaa taaaaagaac
     7621 aaacgtaacg gctgtcccca gtcctaaaga tgtttttacg tttttagata ctgccaaata
     7681 ggaacacatt cccaaaaaat aggcaaatat catgttatcg acaaaaatcg atttaacaaa
     7741 tatattcaat atactttcca tattttattt gttgttttct tgataatatg aataattatt
     7801 caatcgtttt agtagtcata aaaattatat gactattttt cttgcaactc cttgtttttg
     7861 gctctatgaa cccaaatgat acatgccacg agaatcaacg ccataggagg catcatcatc
     7921 agtccgttat tcatgtatcc tacgttaaaa aatgattggg gaataatatt atatcccaat
     7981 agtgtgccag aaccaaaaag ttcacggaaa aatgaaacaa taattaaaat aatagcatat
     8041 ccaaatccat tgcctaaacc atccaaaaaa gattcccagg gactgttgga catggcaaaa
     8101 gcttccagtc gtcccattaa aatacagttg gtaataatca gtccgacata caccgacaat
     8161 tgaacactaa cgtcgtaggc aaaagctttt aaaatttcgc tgactatggt aactaatgca
     8221 gcaacaacta ccagttgaac gataatacga atacgattgg gaatggtatt acgaacaagc
     8281 gaaacaataa tatttgaaaa agcaactatg atggtaacag aaattcccat taccaaagca
     8341 ggctcgagtt ttgcagtaac tgctaatgcc gagcaaatac ccaatacttg aatcgtgatg
     8401 ggattattct tgcttaatgg tcccaataat acttgtcttg actttttgga taataatgta
     8461 ctcattgttt tatttctcct atggttgata attgacgaaa aaacgcttca tattgtccta
     8521 tacagttcag cagcatggtt tctacacctt tgctggtaat tgttccaccg gaaatagcat
     8581 ctacttgttc ccttccttca gcttttttac cgactttcat aaccgcaatc gacacaaatt
     8641 catttttttc gttaaaaata tgtttacctt caaattgtct ttgaaaattc ggctcagcaa
     8701 tgttggcacc caaacccggt gtttcactgg catgagaaaa atatgctccg taaatagtat
     8761 tcttatcatc gtttagtgac acatatcccc aaatcggacc ccaaagacct gttccacgca
     8821 aagaaatgat atattttgtt tctccttcga cttgtgctat aaaaattgga agtcgccttt
     8881 cttcaacttt ttttgataat tctttggcaa tgtcaatatc aaaagcttct ttttttgagt
     8941 aggataaaat ctcgccattg gtattgacaa ttaaaccttt aataacatac tgatcatata
     9001 gttgtgctat attgttctgt ttagcatcga tacgcaaaga ttttaaaatc tgctttttct
     9061 tgtccaattc tatattggaa ttttgctttt ccttcagcgc tcccgataca aatgccagca
     9121 tgagggcaac tattaccacc attactgtag cataaaccag tgtgtaagta ttactgttcg
     9181 tattcatttt aatgtatatt atttttttgg gttattgctg atcccatcat tactttattg
     9241 aatggatttt tgtgctctac ttatccgttt ttttacattg gcatctacaa cataaaaatc
     9301 gataagcgga gcaaaaatat tcataagcaa aattgccagc atcattcctt ccgcgtatcc
     9361 cggattgagt actcgaataa tgatggccag gcatcctatt aataaaccgt atatccattt
     9421 ccctttttcg gtacgggctg aagttaccgg atcggttgcc ataaataccg caccaaaggc
     9481 aaatccacca aaaaccagat ggttgtacca aggaaaatgt gcagcagcag tatcaggccc
     9541 aaatagattg aaaacaatgc ccatcaaagc acctccaaaa aatacagaaa gcatagtttt
     9601 ccaactagcc acttcggtta ttaacaaaac aaaagctccc agcatgatcg ctattataga
     9661 agtttctccc acagaaccgg gaatcaggcc aacgaatcca tcccaaaatc ccaatgagtg
     9721 accaaaagtg tccgttatat ggggaacaga agttgaagca gtagctatct gccccaaagg
     9781 agttgcccct gaaaaagcat ctacaacatt tccgcctcca aatcccaaag cagttccggt
     9841 tcgtatccaa acgctgtctc cggacatgtg agtaggatag ccgaaaaata aaaatgccct
     9901 ggctaccagt gctacattaa aaatattcat tcctgttccg ccaaatactt ctttggcaaa
     9961 aattacggca aatgcggtag ctactgctac catccaaagc ggagtgttaa taggaataat
    10021 taacggaata agaaaacccg ttaccaaaaa accttcctgt atttcctcac cttttatttg
    10081 tgctccaata aattctattc ccaagcccac tgcataagaa actacgatat acggaagaac
    10141 ggccaaaaaa ccgaataaaa attcggtcca aaaatttaca tcctgaccta ttgcaaggta
    10201 gtgttgataa ccaacattat acatgccgaa caaaagagca ggcactaagg caacaatgac
    10261 tgtactcatg gttctcttgg tgtctactgg atcgtgaatg tggctgccgt atttaggtgc
    10321 tgtggtagta ggaacaaata aaaatgtctc aaacccatca taaacagaat gaagtttttc
    10381 taacttgccg cctttttcaa agtacggttt tattttatcc atgaaatttc ttaatgcgtt
    10441 catatccaaa attatttctg cttttttgtt gccgtttaaa cggttgtttt tatgaatttt
    10501 tttattccag ttcttttttc atcatatcca aagcttcacg tacaattagc tgcagaggca
    10561 atttggaagt acaaacaaat tcgcacaaag caacatcttc gggtgctatt tcgtaaccgc
    10621 ctaaagcctc cattttatca atgtttccgg taatcatggc tttaaataga tattccggca
    10681 aaatatccat aggcagtacc cgatcatatt cgttcgacat aataatggct cttcttccgc
    10741 ccaaaatgcg tgcatcataa gaaaatttga tatctccaaa gatttttttg aaaaatttct
    10801 tttgtaacag gggagtaaaa aataatttgg tagcactgaa tttattcaaa cggggcatgg
    10861 cccagcccaa aaattcatga atttctgttc cttcatctat aacaaaaatt tgagaagcgt
    10921 aagggtcaat aaagtcatta atatcaactt ttattccact tattacattt cccaaaatat
    10981 atctcagagg aataccttta tgtacgttac cgtttataaa aggagctact gctgttccgg
    11041 gcagggtagt gtaatatttt ggttcaatta cttctggacc agttaaagct ataatttttt
    11101 ttaaatcaat gatgccctta ttaaaaaatc tgccaatgta aagcatatcc tgaacattga
    11161 cagtccaaac aacctcacct ttgttaatgg gatcgacgtg attgatttgt attcctacat
    11221 ttcctatggg atgaggtcct tcatattcag taatttcaac attttttact ttcaaaaatt
    11281 cgtttgaagg aacattggct tttacgccca aatgaacttt tcccggtgtc aatttggcca
    11341 atgcatcaat tcccgtttga aagtctgcaa actgcccttt caaaataaaa ttgtaatccg
    11401 gagccagtgg agccgtatcg aaagaggaaa taaaaattga ctttggcgtt ttattgggat
    11461 tggctattac atcatagggg cgttgtttga tgtaaggcca aattccagct ttcaacaagg
    11521 ctgatttgat ttcctctcca cttaaacttt ccaatgatgg atgtccaaaa ttttcatatt
    11581 cgttgacaac atcacgctct attgtaatat ttatcaattt tcttttctca cctctgttta
    11641 cagccaatac tttgccgcta acaggcgata cgatgtttaa attttcgtga attttatcgt
    11701 gaaaaaccgg tgtgccggct tttacacgat ctcctgcccg tactaatact tttggaatta
    11761 ttccatgata atagtccgga acgagtgcga caatgtcaga aatcacggta cgctcaatca
    11821 tcttttgagc agcaccatct atagggatat ccaaaccctt ggtagtctta ataacattag
    11881 tcataaaaag aaaaatttgg ttttaaaatt ttggataaaa ttatccctca acttttttgc
    11941 taaaatattt gtcctgaaaa acggtgcaaa attacacatt tttattgtta ttacggagag
    12001 tttattggag aaatttttca ttaatttttc gtggcttctt taaaattgta aatgttatat
    12061 acgattttta tttcagaaat attataatga atcaataaat agacgaagaa cagtgttttg
    12121 cggataaagt tggattaatt ttattatttg agtataattt gaaaggtttt cagaattaat
    12181 tggtttttaa acggcattat agaatgattc ggccaaaagc attcttagac aagacaaatg
    12241 aagaaatccg gttactaatt tgtaggagga aatttcatgg atttggcgta aacatgcaat
    12301 gaagggtaag agaaggatat taaattttgt gtaaattaaa ttatcatgta aatttgccat
    12361 ctgtttttca taaaaactat ggattggaaa gaaatttatt tacaagcaat aaattggcgc
    12421 gaaaaacaca tcaaagaacg ccagtttatc ctgatattaa gttttctgac aggaattttt
    12481 gctgcgttgg cagcttttgt tctaaagatg gccattcatt ccgtgcagtc attggtaaca
    12541 gaaagcttta atcgtgaaag cctcaattat ctttatttaa tcacacctgc agtcggtatt
    12601 attattacat ctctatttgt caggcatata gtaaaagatg atattagtca cggcgtaaca
    12661 cgtattcttt acgccatctc tcaacgaaaa agcatcctta agttgcacaa tgtgtggacg
    12721 tcattggtgg gcagttccat taccattgga tttggtggat cggttggagc cgaagcgcca
    12781 atagttttga ccggttcggc cattggttcc aatttgggaa aatttttcaa aatgaatcaa
    12841 aaaaccatga tgctcctcat cggttgcggt gctgccggtg ctattgcggg tattttcaaa
    12901 gctcccattg ccgggttggt ctttacgctc gaagttttga tgctcgacat gaccatgact
    12961 tccattgtac ctttgctcat ttcgtcggtt acggcaacct cggttaccta tattttgacg
    13021 ggcgatgaat tcatgtttca atttaccaac tacgaacctt ttgccgttga acgcattcct
    13081 tatttgttga tgttgggtgt agtttgcgga ttggtttcgt tgtattttac aaggggcatg
    13141 aactggctgg aaaattttta caaaaagttt gaaaatcctt acatcaaatg ggccattggc
    13201 ggaagtatac tgagtgtttt gatttttctc tttcctcctc tttatggcga aggatacgat
    13261 accatcgcct ccttgttgaa taatgacatt tcttcggtag tcaaacaaag tttttttctc
    13321 ggttcggaaa acaagccttt ggttttactc acctatttgg tgctgattat tgtatttaaa
    13381 atttttgcaa ctgcctctac cacaagtgcc ggtggtgttg gagggatttt tgctccatcg
    13441 ctttttgtgg gttgtattac tggttttgtt gtggccgaaa ttctcaacct gataggtttg
    13501 aatatgccga tagaaaactt cgcattggcc ggtatggcag gtttgatgtc gggtgtgatg
    13561 catgctccgc tgaccggcat ttttttgatt gccgagctta ccggaagtta ttatttgttt
    13621 atgacgctga ttattgtttc caccatttct tatctcacca ttattctttt cgaaccacac
    13681 agtctttatg ccatgcggct ggctcaaaaa ggcgagttga ttacgcacaa caaagacaag
    13741 gctgtattga ccctcctgaa aatggaaaat gtaatagaaa caaacctgaa cgtattggaa
    13801 ccggacatga aactgggaga agtaattaaa ataatttcgc attcgaaacg aaatgttttt
    13861 ccggtggtaa atccgaaaaa tggagttctt ttggggattt taacgctcga cgaaatcaga
    13921 aatatcatgt tccgacctga attgtaccag cgtttcacgg tacgtcagct catgatttca
    13981 cctccgactt ttctcaatgt gaacatgcca atggaaaaag tcatgcagat ttttgaagac
    14041 acgggagcat ggaatcttcc cgtcgtggac gacgaaaaac attatatcgg atttgtttca
    14101 aaatccacca tattcaattc ttatcggcat gttctggtac atttttccga tgaataaccg
    14161 aagaatttat tatcgacaaa caaccaacat ttaaaataag atgaccaaaa acaaacctac
    14221 ttacgacgaa gccattgctg aattggataa aattcttgca gaactggaaa ataactcgga
    14281 catcaacatg gatgaaatag ctcaaaaagt aaaacgagct tccgaattat tgcaaatatg
    14341 taagaaacag ctgcacgtac tggatgaaaa tctggaaaaa ctcctctcag agttggaaga
    14401 ataattattt gacaagtgta atattcactt cttttgcttc agcatatttt tcgagcaaca
    14461 ttctggcctg ctcggacgct tcttgcaacc ctttttctcc gctataacca ttgataatca
    14521 ttaacagacg gcgagtttcc attgttaatt ttgttcgttc gttgtcgctg gtaggcgttc
    14581 cgatacctcc tgcagcatct ttgaaaagcg gtaatccttc cacattcaaa ataccgcgac
    14641 caattgcttg aaaaatatcg tttgccgttc ccacttccag ccgaaggtct ccctgaattt
    14701 tttttgcatc aaatcctcca atggaaaatc cgcttcggat ggaaatcagg ttgatgatat
    14761 ccaccaatgt gcttactcgc tgaataccca ctcctttgca aattcttctg cacagcgctt
    14821 cggccgaagg acgatagcga ttgggatcct ttcccaactt tctgtaaact tggcgcgtag
    14881 cggaaatttc gggacgcatt tttatatttt ccaatgtaaa agttgaagaa attttctcca
    14941 tttcctcttc tatctcttgc cacaaaatac ttgtggtttc ggaatttttc acttcgcagg
    15001 cgataacgct taaagtcaaa tcgggacaag cctgacgaat aagttcgcta atgtagatct
    15061 gcattggatt atgcttgttt taacatgatg ccgctcaaca ttcgtccgca tagaatacct
    15121 tgacgccggg cagaaaaaaa gttttccgat tcggtgaatg tacagcgaca cagaaactca
    15181 atattatttt tttgtaatcc gcttcccagt aatgatattt cgttggcttt ccataaattg
    15241 agaaaaattt tcccatcatt ttgggagaaa atgtccgaca aattccaatc gtttttctga
    15301 aattcttcta ccacatccct gccaacttca tataccgaag gcgaaatgga aggccctgct
    15361 accacaaaaa tatcttccg
//
LOCUS       DAOSCH010000046        15191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2677_length_15191_cov_5.578554, whole genome shotgun sequence.
ACCESSION   DAOSCH010000046 DAOSCH010000000
VERSION     DAOSCH010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 15191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15191
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2677_length_15191_cov_5.578554"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1382)
                     /locus_tag="PLZ54_05020"
     CDS             complement(<1..1382)
                     /locus_tag="PLZ54_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOJ66325.1"
                     /translation="MCGIAMIRLLKPLDYYQKKYGTWEYGLQKMYLLMEKQHNRGQEG
                     AGIATVKLDMPPGEEYISRKRIEGKNAIQEIFVDIDKNHSLAPEDYSSDAAKAKMFCP
                     FAGELYLGHLRYSTSGKSGLSYIHPFLRRHNWKSRTLVLAGNFNLTNVDEIFQSLTAQ
                     GQHPRDKSDTFLMLESLGDKLDREVQREYEIIKQRYSNDQVITRKIEENLDIASFIRK
                     SEHIWDGGYVICGMLGHGDAFLFRDPKGIRTAFYYADDEIIVAASERPVIQTAINVKA
                     EQVHELKPGEALIIKKNGSMHFETIREADPEPKPCSFERIYFSRGSDKDIYQERKKLG
                     GFLSEAILKAVDQDIDNTVFSFIPNTAEVAFYGMTDGIRTLTGKVPRIEKVLLKDIKL
                     RTFISQNKERNDLAAHVYDTTYGSIRPEKKDNLVVIDDSIVRGTTLKQSILNILNRLE
                     PKKIVIVSSSP"
     gene            complement(1438..3279)
                     /gene="glmS"
                     /locus_tag="PLZ54_05025"
     CDS             complement(1438..3279)
                     /gene="glmS"
                     /locus_tag="PLZ54_05025"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOJ66326.1"
                     /translation="MCGIVGYMGYREAYPILIKGLQRLEYRGYDSAGIALLNDHKLTV
                     YKTRGKVSDLIHSVENKDISGTVGIAHTRWATHGEPNNANAHPHCSQSEELAIVHNGI
                     IENYAVLKQGLIEHGYTFSSETDTEVLVQLIEYVKRKNNVDLPTAVQLALNQIVGAYA
                     IAVIEKGHPDVIVAARKGSPLVVGIGDNEYFLASDATPIIEFTHKVVYVGEEEIVVLQ
                     RGKDLKIKTIGNVEKTPEIKELEMTLSQLEKGGYPHFMLKEIFEQPKTLSDSMRGRIN
                     LEENNISLSGFIDHKEKFLDAKRIIITACGTSWHAGLIGMYAIEEFARIPVEVEYSSE
                     FRYRKPVINKDDLVIAISQSGETADTLAAVQLAKSHGAFIFGICNVVGSSIARNTDSG
                     CYTHVGPEIGVASTKAFTAQVTVLIMLALLIGKEKGTIENEQYLKIVGELAKIPEKIS
                     QILKQNDVIAEFSKTFTYAQNFIYLGRGYNYPVALEGALKLKEISYIHAEGLPAAEMK
                     HGPIALISQEMPVVVIAPSAGKYEKVVSNIQEIKARKGRIISIVTEGDVIVKNLSDYI
                     ISIPETVECLVPLLAVIPLQLLAYHIAVVKGCDVDQPRNLAKSVTVE"
     gene            complement(3316..4542)
                     /locus_tag="PLZ54_05030"
     CDS             complement(3316..4542)
                     /locus_tag="PLZ54_05030"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOJ66327.1"
                     /translation="MALANENYLKIPESRFSDEIEKRVNAFKVLHPTTKLIRLGIGDV
                     TRPLPAESVFAIHKAADEMLHTETFKGYGPLQGYDFLVDKILKNYRSSGIVLDKESIF
                     INHGAKPDIGNIGHILGRDNIIAIAEPVYPVYENAAIMSGRGGEMNEEEKWSNIVYLH
                     CSKENNFVPELPDERVDIIYLCNPNNPTGTVINKTELKKWVDYAIENQSIIIYDGAYH
                     AYIKEPDIPHSIYEVKGAKKVALEIRSYSKTAGFTGLRCGYTVFPKELMVYTRTGEEI
                     PLLKLWYRRNLNYSNGVSYVVQRAAEALYSRKGRMEIQELVNYYLENAKKIRKELLEL
                     GWEVYGGINSPYVWFKTPHNEPSWKFFQELLYNYNIIGMPGIIFGKSGDGYMRFSGFC
                     SHEDTEEAIKRIKSNY"
     gene            5047..5592
                     /locus_tag="PLZ54_05035"
     CDS             5047..5592
                     /locus_tag="PLZ54_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOJ66328.1"
                     /translation="MNQPTSAIISEELIDEIIGKYQNKPGFLLSVLEEIQEKNPLKYL
                     PAETLQTVAAKLNLPYSRVYGVVTFYSFFNLRPQGKHSIVVCRGTACHTKGSKQLLNE
                     IGHSFGMNHQDENSEASFTTADNQFTVRTVACFGQCAQSPVVEVDGKIYSYVNSQKLL
                     KILENVKKNGVQKNFSVNTSR"
     gene            5611..8748
                     /locus_tag="PLZ54_05040"
     CDS             5611..8748
                     /locus_tag="PLZ54_05040"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ66329.1"
                     /translation="MTVAELELLKNKNIERILPSVPMFLVGMSTCGLGNGAESVYRIL
                     EKEIELRHVNFKLKQTGCFGYCAEEPIVMMYQPQKPLVVYSRVNEKDISAIVDGIIKS
                     KIYRKKILCRIDFWDHLTSKIEYGKDYEDVPHWDEISFFKGQKKIVLRDAGFIDPESI
                     LDYIAVGGYQALSKVLTGKQPNEVIDEVTKSKLRGRGGAGFPTGTKWSIMQHSKADVK
                     YIVCNADEGDPGAYMNRNEIESDPHALIEGMIIGAYAMEANQGVIYIRAEYPLAVKRF
                     QMAMQQAREYGLLGNNILNTGFNFDIQLVEGAGAFVCGEETALIASIEGKAGRPMPRP
                     PYPAERGLYGKPTNINNVETWCNIPVIIEKGSDWFTKTGTERSAGTKVFSLVGKIKNT
                     GLVELPLGSTLEKFIFEIGEGTGNNKRIKAVQTGGPSGGCIPVEYFSTPVDYESLSSL
                     GAIMGSGGMVVMDQDNCMVDVAKYFLEFSANESCGKCTPCREGLSQALAIMNKISKGE
                     GNFQDIETLELLTRTIKDTAICGLGQTAPNPILTTLRYFKNEYEEHIQEKRCHAGTCE
                     SLFLALCENSCPLHMNIPGYIELVKEDRIEEAFELTLRDNPIPGTIGRICHFHCQMRC
                     RREMVDEPVAQGEIHRYLADTMYQLGKENEIYNRLIAEKLPSSGKKIAIVGAGPAGIT
                     AAFYLVRLGHEVTVFDAMPEAGGVLRYGIPQYRLPKSVLKKELKLIKKLGVKFVFNTR
                     IGKDIFLQDLKNNFNAVYLAFGAWKDIDLNIPGENAKGVFVGTDLLKELSLDKTPRIG
                     QKIVVIGGGNVAIDAARSLWRLGKDVTVVYRREKIDMPANKDEIAEAEAEQIHFRFLS
                     APHEILTDNSGRVNALKIEIMTTGPVDTSGRRKPIATGKIEEIPCDTVVLAVGERVDS
                     APFVAEGLKVTKDGRLIVHPFTFQTSEPNIFAGGDAVMGPATAAEAMGMAKRAAEAID
                     WELMKEKRFHLLFKKFEYRNVIPTDIKKSPKNVSAKIQVKERIGNFHEVSTGYTGEQA
                     RDEVNRCLRCDVKVN"
     gene            8837..10594
                     /locus_tag="PLZ54_05045"
     CDS             8837..10594
                     /locus_tag="PLZ54_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOJ66330.1"
                     /translation="MKITINHQPVDVKDGITIMEACQSVGIDIPSLCFLKDISSNASC
                     GICVVEVKGARSLLRSCITKVAEGMDILTTSPRVLEARKVNVELLLANHPQDCLQCDR
                     NLNCELQRLSFELGIKERHFVRTRKKYLPKDETSLSLVRDPEKCILCGRCVAVCTDMQ
                     SVNAIDFSGRGLNTSISTFFDKGLGATYCTNCGQCSLVCPTGAITEKTSVPQVWKEIL
                     NPEKMVLIQTAPAVRVGIGEAMGMPYGSLVTGQMVAGLKRLGFSKVFDTNFAADLTIM
                     EEANELMQRIRQNGVLPMITSCSPGWIKFAEHFFPSSLAHLSSCKSPQQMFGAIAKTF
                     YAEQTGIDPRKIVVVSVMPCTAKKYEARRPEMDDAFHYWKEKLQLSEEEHFFDVDYAL
                     TTRELARMFKESGIDFQHLLEEDFDQPLGVSTGAGTIFGATGGVMEAALRTAYEVMTG
                     RQLKDVEFLSVRGMKGIKEAEIDLDGRKIKVAVAHTLKNARFLLEQIQAGISPYTFVE
                     IMTCPGGCLGGGGQPIPTNSEIREKRAASIYREDERKQIRKSHENPEIQYLYEHFLEK
                     PLSEKSHHLLHTHYVERGC"
     gene            10700..11176
                     /locus_tag="PLZ54_05050"
     CDS             10700..11176
                     /locus_tag="PLZ54_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dps family protein"
                     /protein_id="HOJ66331.1"
                     /translation="MKTIDCTKLNAEGAKKVVDALQQLLADYQIFYTNLRGFHWNLKG
                     KDFFINHAKFEELYENVAEKVDEIAERILMLGGTPAHNFSDYLKTAKLKETGLVTDGD
                     TGLKNVLESYAYFIESERNIIKLAEAIDDEATVAQMSDYIKEQEKMVWMLVSYFEK"
     gene            11368..12441
                     /locus_tag="PLZ54_05055"
     CDS             11368..12441
                     /locus_tag="PLZ54_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOJ66332.1"
                     /translation="MEQNYCIIMAGGIGSRFWPFSRKNRPKQFLDFFGTGRSLLQLTY
                     DRFRKIIPAENILIVSNELYRDLILEQLPEINDNQLLLEPVRRNTAPCIAYAIYKIKS
                     ITENANIIVSPSDHLILKESEFLETIRQGLEFVSKNDNLLTLGIKPSRPETGYGYIQI
                     EFGESNLRKVKTFTEKPNAELAKLFFESGEFFWNSGIFLWNLKTILAAFDFHLSEISQ
                     KFKQGLKYFNSTEEKKYIADIFHTCPNISIDYGILEKASNVFVLCSDFGWSDLGTWGS
                     LYDISPKDEKENVTLKCKTLYYDSHRNIVALPSGKLAVIQQLNDYIVADSDNVLLICK
                     REDEQQIRQFVKDASILYDGEFD"
     gene            12471..12755
                     /locus_tag="PLZ54_05060"
     CDS             12471..12755
                     /locus_tag="PLZ54_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66333.1"
                     /translation="MTPSFFNIYKPRKYDYRYIYYDPLKEAIKEREKQMAKENGEQPQ
                     QEYKPNLHRGSFREEANRMRGKKSKELRRSNRNVLIILIILLIIAYYLLK"
     gene            12771..14606
                     /gene="mutL"
                     /locus_tag="PLZ54_05065"
     CDS             12771..14606
                     /gene="mutL"
                     /locus_tag="PLZ54_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOJ66334.1"
                     /translation="MSDIIHLLPDSVANQIAAGEVIQRPASVLKELIENSIDANANNI
                     IIQIKNAGRTLIQVTDNGKGMSETDARLAFERHATSKISNVSDIYALQSYGFRGEALA
                     SIAAVAQVELQTRRKEDELGTLIEIEGSKVIRQESVLCEEGTTIRVKNLFFNVPARRR
                     FLKSDNVEKIHLLNEFYRIALVYPDIRFSFFDDKEEIFKLLPSPLKLRIENIFGKGTK
                     RKWEQQLLPIETNTPLVKIEGYVGRPEFALKSAYQYFFVNARYMRHPYFHKAVISAYS
                     QMIRPEENPNYFIYLYVAPETIDVNIHPAKTEIKFENEQAIWSILLATVRETLGKYNV
                     IPSINFNQEDAIDLPIIGNNEKIVPPHTVFNPNFNPFENTLINKRPPLNWETLYKNFE
                     KKEESKPTSFQWIENDITAYASSASSIIAEETLDSSHFLQLKTKYILTAVKSGLLVIH
                     QHKAHVRILYDLYLSQIKHQKSSSQQLLFPEILELSEHEMLYFSSIKNDLSNVGFLFK
                     SVGKNAFEIFGIPGPLDKMNIQAFIHELIEKFKVKQSISEEDLHQQIALSMAETSAIK
                     TGQKLTQEEMTDLVNRLFATEEPSFSPQGEKILSIIPMEEFDSQF"
     gene            14790..>15191
                     /locus_tag="PLZ54_05070"
     CDS             14790..>15191
                     /locus_tag="PLZ54_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HOJ66335.1"
                     /translation="MKLIDEKLLNELTRQAKENARLRVNYNLHEHLEDKVQRLLNAME
                     PGTKLPIHRHRHTAETYFLIRGSILVKYYDDKGNLTEETELNVKEGKYGIHIPVGQFH
                     TLEVLESSTIIFEVKEGPYQPIGDEDILKSDI"
BASE COUNT         4714 a         2868 c         2996 g         4613 t
ORIGIN      
        1 ggagatgagg acacaatgac gatttttttg ggttccaatc gattcaatat atttaaaata
       61 ctttgtttga gcgttgtacc ccgaacgatg gaatcgtcaa tcactaccaa attgtctttt
      121 ttctcgggtc ggatggagcc ataggtagta tcgtaaacat gagccgccaa atcgtttctt
      181 tctttgtttt gcgaaataaa ggtgcgcaat ttgatgtctt tcagcagcac tttttcgatt
      241 cgtggaactt tgcccgtcag agtgcgaatg ccatccgtca ttccgtaaaa agccacttcg
      301 gccgtattgg gaataaacga aaacacggta ttatcaatat cctgatcgac tgcttttaaa
      361 atagcttcac ttaaaaatcc tcccaatttt tttctttcct gataaatatc tttgtcgctt
      421 ccacgtgaaa aatagattct ttcaaaagaa caaggttttg gttcgggatc tgcctcacga
      481 atggtttcga aatgcatcga tccgtttttc ttgataataa gtgcttctcc gggttttaat
      541 tcatgtacct gctctgcttt cacattaatg gctgtttgaa tgaccggacg ttcggaagca
      601 gcaacaataa tttcatcatc ggcgtagtaa aatgcggtcc taattccttt ggggtcgcgg
      661 aaaaggaagg catcgccgtg tcccaacatc ccgcaaataa cgtaacctcc atcccaaatg
      721 tgttccgact ttcggataaa agaagcaatg tccagatttt cttcaatttt tcgggttatc
      781 acctgatcat tggagtagcg ttgtttgata atttcatatt cacgttgtac ttctctgtcg
      841 agtttgtcgc cgagcgattc cagcatcaga aaagtatccg atttgtcgcg tggatgttgt
      901 ccctgtgccg ttagcgactg aaaaatttca tcaacattgg ttaggttaaa atttccagcc
      961 aacaccaatg ttcgcgattt ccaattgtgt ctgcgcagga acggatgaat atatgacaat
     1021 ccgcttttcc cgcttgtgct gtagcgcaaa tgtcccaaat aaagttctcc ggcaaacggg
     1081 caaaacattt ttgctttggc agcatccgaa ctgtaatctt cgggcgccag cgagtgattt
     1141 ttatctatat cgacaaaaat ttcctgaatg gcattttttc cttctattct tttacgcgaa
     1201 atatattctt ctccgggcgg catatccaat tttacggtgg cgattcctgc accttcctga
     1261 ccccgattgt gctgcttctc cataagaaga tacatttttt gcaaaccgta ttcccaagta
     1321 ccgtattttt tctggtaata atccaatggc ttcaatagcc ttatcattgc gattccgcac
     1381 ataataaatt tcttatttta tttaaaagag attataagat ttttgaaact caatatttta
     1441 ttctacagtt actgatttcg ccagatttcg gggttggtcc acgtcacaac cttttaccac
     1501 agcaatgtga taggccaaaa gttgcaacgg aatcacagcc agcaaaggaa ccaagcattc
     1561 tacggtttcg ggaatggaaa taatgtaatc cgaaagattt tttacaatta catcgccttc
     1621 cgttacgata gaaataattc taccttttcg tgcttttatt tcctgaatgt tgctgacaac
     1681 tttttcgtat tttcctgcac tgggtgctat tacaacaacc ggcatttcct gactgatcaa
     1741 ggcaattggt ccgtgtttca tttcggctgc cggcagtcct tcggcatgaa tataggaaat
     1801 ttccttcaat tttaaagctc cttccaaagc taccgggtaa ttataacctc tacccaaata
     1861 aataaaattt tgcgcatagg tgaatgtttt ggaaaattcg gcaatgacat cattttgctt
     1921 taaaatttga ctgatttttt cgggaatttt tgccagttct ccaacaattt tcaggtattg
     1981 ttcattttca attgttccct tttcctttcc gataagcaat gccagcataa ttaaaactgt
     2041 aacctgagct gtaaaagctt tggtggacgc cacaccaatt tcggggccaa cgtgtgtata
     2101 acagcccgaa tcggtattgc gtgcaatgga tgagcccacc acgttgcata ttccgaaaat
     2161 aaatgctccg tgcgatttgg ccaattgtac ggctgccagt gtgtcggcag tttcgcccga
     2221 ctgagatatg gctatcacca gatcatcctt gttgataacc ggcttgcggt atctgaattc
     2281 cgaagaatat tcaacttcaa ccggaattct ggcaaattct tcaatggcat acattcctat
     2341 taagccggca tgccatgatg ttccacaagc ggttataata atccgtttgg catccaggaa
     2401 tttttccttg tggtctataa atcctgaaag actaatgttg ttttcttcca aattgattcg
     2461 ccctctcatg ctgtcgctca aggttttggg ctgctcgaaa atttctttca gcataaaatg
     2521 tggataaccg cctttttcga gctgactgag agtcatttcg agttctttga tttcgggagt
     2581 tttttctaca ttcccaatgg ttttaatttt taagtctttt ccgcgttgca aaacgacaat
     2641 ttcctcttct cccacataca ccaccttatg cgtaaattca atgatgggag tggcatccga
     2701 agccagaaaa tattcgttat cgccaattcc cacaacgagc ggactgcctt ttcgagctgc
     2761 cacaattaca tcgggatgac ctttttcgat aacggcaatc gcataagctc ctacaatttg
     2821 attcaatgcc aattgcactg cagtaggcaa atccacgtta tttttccgtt ttacgtattc
     2881 tatgagttga accagcactt cggtatcggt ttcgctcgaa aaagtatatc catgctcgat
     2941 aagtccctgt ttcaaaacgg catagttttc aattattccg ttgtgtacaa tggccagttc
     3001 ctcagactga gaacaatgtg gatgtgcatt tgcattgttg ggttcaccat gtgtcgccca
     3061 tcttgtatga gcgataccga cagttccact gatgtctttg ttttcgacag aatgaatcaa
     3121 gtcggaaact tttcctcttg ttttataaac agttaattta tggtcgttaa gcaatgcaat
     3181 gccggcgctg tcgtagccac gatattcgag acgctgcaag ccttttatta aaatgggata
     3241 agcctcgcga taacccatgt aaccaacaat accacacata aagttaatga tttaagattt
     3301 ttcttagata attttttaat aatttgattt gattcgttta atggcctcct ctgtatcttc
     3361 atgactacaa aatccggaaa aacgcatata accatcaccc gattttccaa aaataatacc
     3421 gggcattcca ataatgttgt aattgtataa aagttcctga aaaaatttcc acgaaggttc
     3481 gttatgaggc gttttaaacc aaacatacgg agaattaatg cctccataaa cttcccagcc
     3541 aagttctaaa agttctttac gtatcttttt tgcattttcc aaatagtaat tgaccagttc
     3601 ttgaatttcc attcttccct tacgactgta caaggcttcg gcagcacgct gaactacata
     3661 agatacacca ttgctgtaat tcagatttct gcgataccaa agtttcaaca acggaatttc
     3721 ctcgcccgtc cgggtataaa ccatcaattc tttcggaaaa accgtgtaac cacatcgaag
     3781 ccctgtaaaa ccggctgttt ttgaataact tctgatttcc aaagcaacct ttttggcacc
     3841 cttgacttcg taaatgctgt gaggaatatc gggttctttg atataggcat gataagctcc
     3901 atcatatata ataatacttt ggttttcaat agcataatct acccattttt tcagttcggt
     3961 tttgttgatt acggttccgg tgggattatt ggggttacaa agataaatga tgtctacgcg
     4021 ttcgtcaggg agttcgggaa caaaattatt ttctttcgaa caatgcaggt aaacaatatt
     4081 gctccatttt tcttcctcat tcatttcacc gcctcttcca ctcataatgg cagcattttc
     4141 gtaaaccgga taaaccggct cagcaatggc aataatgtta tctcgtccca aaatgtggcc
     4201 gatatttcca atatcgggtt ttgcgccatg attaataaaa atactttcct tatcgaggac
     4261 aatgcctgaa gaacgataat ttttgagaat tttatcgacc aaaaaatcat acccttgcag
     4321 tggcccgtaa cctttgaaag tttccgtatg caacatttcg tctgcagctt tgtgaatggc
     4381 aaaaacagat tctgcgggta aagggcgtgt aacatctcca atccccaaac gaatcaattt
     4441 ggtggttgga tgcaaaactt tgaaggcatt tacccgcttt tctatttcgt ccgaaaaacg
     4501 ggactcggga attttcaagt aattttcatt tgctaatgcc attcaggaaa tgattaagca
     4561 ggaatttttt cctataaata aagcacaaaa ttactgtttt ttcattgatt atttttcttt
     4621 caattcaaaa tttcaactat acatttcagt atttttgtgt ttttaatctc cttttccaat
     4681 aaaaacaaac ttattcatct aattttattt cattatccaa tttttttctt tcaaaatgta
     4741 acttttattt ttaataaatc taagttcttc cacatgtttt aaaaatgaaa gaaaaatatt
     4801 gatttttact tttattaaaa aaaattattt tataatcata acaaaataaa aatgtgtcaa
     4861 ttaaagaaat ttttccataa ataaatctaa aaaattgaca ataatcattt aattttatat
     4921 ttttgtttat agaaatattc aaagtatact gaaacatcgg atgattgaaa ttatatattt
     4981 ttcttcagat tttcaatgac ttatttataa attattagtc aaataacttt taaaaatgaa
     5041 ggttttatga accagccaac ttctgcaatt atttccgaag aacttattga tgaaattatt
     5101 ggcaaatatc aaaataaacc cggatttttg ctttcggtgc tggaagagat tcaggaaaaa
     5161 aatccattga aatatttgcc tgcagaaacg cttcagactg ttgccgcaaa actcaatttg
     5221 ccctattcgc gagtttatgg cgttgtaact ttttattctt ttttcaatct tcgtccgcag
     5281 ggaaaacatt ccattgtggt gtgcagggga acagcctgtc ataccaaagg ttcgaagcag
     5341 ctgcttaacg aaatcggaca ttctttcgga atgaatcatc aggatgaaaa ttctgaagct
     5401 tcttttacaa ctgccgacaa tcagtttaca gtgcgaacgg tagcctgttt tggacagtgt
     5461 gcacaatcgc ctgtggtaga agtggatggg aaaatctaca gttatgtaaa ttctcaaaag
     5521 ctgctgaaaa ttctcgaaaa tgttaaaaag aacggagttc aaaaaaattt ctctgtaaat
     5581 acatctcgtt aaccttaaaa tgttatggct atgacagtgg cagaacttga attgttaaaa
     5641 aataaaaata ttgaacgaat attgccttct gttcccatgt ttctggttgg aatgagcact
     5701 tgcggcttgg gaaatggagc agaatcggta tatcggattt tggaaaagga aattgaactt
     5761 cgccatgtta atttcaaact caaacaaacc ggttgttttg gttattgtgc agaagaacca
     5821 attgttatga tgtatcaacc tcaaaaacca ttggtagttt acagccgcgt aaacgaaaag
     5881 gacatttctg ccatcgttga tgggattatt aaatcaaaaa tttaccgaaa aaaaattctt
     5941 tgtcgtatcg atttttggga tcatttgact tctaaaatcg aatatggaaa agattacgaa
     6001 gacgttccac attgggacga aatttccttt tttaaaggac agaaaaaaat tgtgttgcgt
     6061 gatgcaggtt tcatcgatcc agaatctata cttgattata ttgctgtggg tggctatcaa
     6121 gctctttcaa aagtattgac cggcaagcag ccaaacgaag tgattgacga agtaacaaag
     6181 tcaaaactca gaggtagagg aggagcgggg ttcccaaccg gcacaaagtg gtcaattatg
     6241 caacattcaa aagccgatgt taaatatatt gtttgtaatg cggatgaagg tgatccggga
     6301 gcttatatga acagaaacga aattgaaagc gatccccatg cattgataga aggaatgatt
     6361 atcggagctt acgcaatgga agctaatcag ggagttattt atatcagagc cgaatatcca
     6421 ttggcggtaa aaagattcca aatggccatg cagcaagcca gagaatacgg attgctgggc
     6481 aataatatat taaataccgg ctttaatttc gacattcaac tggttgaagg agccggagca
     6541 tttgtttgcg gagaggaaac ggcattaatt gcttctatcg aagggaaagc aggccgtccc
     6601 atgcctcgac ctccttatcc tgccgaaaga ggattgtacg gcaaaccgac caatatcaac
     6661 aacgttgaaa catggtgcaa tatcccggtg attattgaaa agggaagcga ttggtttacc
     6721 aaaacgggaa cggaaaggag cgccggaaca aaagtatttt cgttggttgg aaaaattaaa
     6781 aatacaggtt tggtcgaact tccgttaggt tcaactttgg aaaaatttat ttttgaaata
     6841 ggcgaaggta cgggaaacaa caaacgtatc aaagccgttc aaaccggtgg cccatcagga
     6901 ggctgtattc cggtggaata tttcagtacg ccggttgatt atgaatcttt atcaagtctg
     6961 ggtgctatca tgggctcggg cggaatggta gtaatggatc aggataattg catggtggat
     7021 gtggcaaaat attttttgga attcagtgcc aacgaatcat gcggcaaatg tacgccttgc
     7081 cgcgaaggac tgtcgcaagc gctggccatt atgaacaaaa tatccaaagg agaagggaat
     7141 tttcaggaca tcgaaacttt ggaattattg acccgaacca tcaaggatac tgccatttgc
     7201 ggcttgggac aaaccgcgcc caatcccatt cttactactt tgcgttattt taaaaacgag
     7261 tacgaagaac atattcaaga aaaacgttgc catgccggta cctgtgaatc gttgtttctg
     7321 gcactttgtg aaaatagctg tccgttacac atgaacattc ccggctatat cgaattggtt
     7381 aaggaagacc gtattgaaga agctttcgaa ctcactttgc gcgataatcc catacccggt
     7441 accattggca gaatttgcca ttttcactgt cagatgcgtt gtcgacgcga aatggttgat
     7501 gaaccggttg ctcagggcga aattcatcga tatttggccg ataccatgta tcagttgggg
     7561 aaggaaaatg aaatttacaa ccggctgatt gccgaaaaac ttccttcctc gggtaaaaaa
     7621 attgccattg ttggtgccgg tccggctgga attactgcgg cattttattt ggtaagactc
     7681 ggacatgaag ttaccgtttt cgatgccatg cccgaagccg gaggtgtttt acgttacgga
     7741 atacctcaat atcgccttcc taaatcggta ctgaaaaaag aactgaaact gataaagaaa
     7801 ttaggcgtta aatttgtatt taatacgcgt atcgggaaag acattttttt gcaagatttg
     7861 aaaaacaatt tcaatgcggt ttatcttgct tttggagcat ggaaagacat tgatttgaat
     7921 attccgggag agaatgccaa aggagttttt gtcggaacgg atttattgaa ggaactttcg
     7981 ctggacaaaa cgcccagaat tggacagaaa attgttgtta ttggcggagg caatgtggcc
     8041 attgatgcag caagaagttt atggaggttg ggaaaagatg taacagtggt ttatcgtaga
     8101 gaaaaaattg atatgcccgc caataaagac gaaattgccg aagctgaagc agagcagatt
     8161 cacttcagat ttttgtctgc cccacatgaa attctgaccg ataattcagg aagagtcaat
     8221 gctttgaaaa ttgaaattat gactacaggg ccggtcgata cttcgggcag aaggaaaccg
     8281 attgcaaccg gtaaaattga agaaattcct tgcgatacgg ttgtgctggc tgtgggagaa
     8341 cgtgttgatt ccgctccttt tgtagcagaa ggtctgaaag ttacgaaaga cggaagatta
     8401 attgttcatc cttttacttt ccaaacttct gagccaaata tttttgccgg aggagacgcc
     8461 gtaatgggac cggcaacggc tgcagaagct atgggaatgg ccaagagagc agcagaagcc
     8521 atcgattggg agttaatgaa ggaaaagcgt ttccatttgt tattcaaaaa atttgaatat
     8581 cggaatgtga ttcccacaga cattaaaaaa tcgccgaaaa atgtttctgc aaaaatacag
     8641 gttaaggaac gaataggtaa ttttcatgag gtttcgacag gttacaccgg cgaacaagct
     8701 cgagatgaag ttaaccgctg tttgcgttgt gacgtgaaag tcaattgatt atctagaaaa
     8761 ttatttttca tttctatttt ttccatactt caacgaaact taaaaaataa ccttatttga
     8821 aaattataat caatctatga aaatcacaat taatcaccaa ccggttgatg taaaagatgg
     8881 aatcactata atggaagctt gccaatctgt cggcattgac attccttcgc tttgttttct
     8941 caaagatatt tccagcaatg cttcttgtgg catttgtgtg gtagaagtaa agggagcgcg
     9001 cagtttattg cgttcgtgca ttacaaaagt tgctgaagga atggatattc ttaccacatc
     9061 accacgtgta ttggaagcac gaaaagtcaa cgttgagctg cttttggcca atcatccgca
     9121 agattgcctg caatgcgaca gaaatttaaa ttgtgaattg caacgtttat cctttgaatt
     9181 gggaattaaa gaaagacatt ttgtacgtac cagaaaaaaa tatcttccca aagatgaaac
     9241 ttctctttcg ctggttcgcg atcccgaaaa gtgtattttg tgcggtcgtt gcgttgccgt
     9301 ttgtaccgac atgcagtctg tgaatgccat tgatttttcg ggtagaggat taaatacgag
     9361 catttcaact tttttcgaca aaggattggg tgcaacctat tgtaccaact gtggccaatg
     9421 ttcgctggtt tgtcccacag gagccattac cgaaaaaaca tctgttccac aagtttggaa
     9481 agaaatactc aatccggaga aaatggtact tattcagaca gctccggctg tgagagtggg
     9541 gattggcgaa gcaatgggca tgccttatgg aagtttggta accggacaaa tggttgccgg
     9601 tctgaaaaga ttgggtttct caaaagtttt cgataccaat tttgcagccg atcttaccat
     9661 aatggaagaa gcaaacgaat taatgcaacg tattcggcaa aatggagttt tacccatgat
     9721 aacgtcctgt tcgcccggat ggataaaatt tgccgaacat tttttccctt catctctcgc
     9781 tcacttgtct tcctgcaaat cgccccagca aatgtttggt gccatagcca aaacttttta
     9841 tgctgaacag accggtatcg atccgcgaaa aattgtggtt gtttcggtaa tgccgtgcac
     9901 agcaaaaaaa tatgaagccc gccgacctga aatggatgat gcgttccact attggaaaga
     9961 gaaattacaa ctttcggaag aagagcactt ttttgatgtc gattatgcac tgactacgcg
    10021 agaattggca agaatgttca aagaaagcgg aatagatttc caacatttac tagaggaaga
    10081 tttcgatcag ccgttgggtg tttctaccgg agccggaaca attttcggag caaccggcgg
    10141 cgtcatggaa gcggctttaa gaaccgctta tgaagttatg acaggtcggc aactgaagga
    10201 tgttgaattt ttgtcagtca gaggtatgaa aggcattaaa gaggcggaaa ttgatctaga
    10261 cggaagaaaa attaaagttg ccgtagctca cacattgaaa aatgcacgtt ttttgctgga
    10321 acagattcaa gcagggattt ctccctatac attcgtggag attatgactt gtccgggagg
    10381 atgtttggga ggtggaggtc agcctattcc tacaaattcc gaaataaggg aaaagcgggc
    10441 agcttccatt tatcgtgaag atgaacgcaa acaaatacga aaatctcacg aaaatcccga
    10501 gatacagtat ctttacgaac attttttgga aaaaccgttg agtgagaagt cacatcattt
    10561 attgcatact cattacgtag aaaggggctg ttgaattata gatattttct attatttcat
    10621 ttatgttttc agtttgattt attgttttaa acatataatt ttgccttaaa taattaatca
    10681 aaaaaaggat gtatttatta tgaaaacaat tgattgtaca aagctgaatg ctgaaggagc
    10741 taaaaaagtg gttgatgcct tacaacaact tttggccgat tatcagattt tttacaccaa
    10801 tttgcgtgga tttcactgga acttgaaagg taaagatttt ttcatcaacc atgccaaatt
    10861 tgaagaattg tatgaaaatg tagccgaaaa agtggatgaa attgcagaac gcattttaat
    10921 gttgggtgga acgccggcac ataatttcag tgattatctt aaaacagcca agttgaaaga
    10981 aaccggtttg gtaactgatg gcgacacagg attgaaaaac gtactcgaat cttacgctta
    11041 ttttattgaa agcgaaagaa atatcattaa attggctgag gctatagacg atgaggctac
    11101 ggtggctcaa atgagtgatt atattaagga acaggaaaaa atggtttgga tgctggtatc
    11161 ctattttgaa aaataaagaa tgaaccatgt caaaattaaa aaacggggtt gatattcaat
    11221 caatcccgtt ttttcgttca gcctgatttt ttctagcatg ttaccaaatt tctacaatta
    11281 ttttggcaaa aaaagaaaaa cactgtatct tagtggtgtt attttgttat aagcattttt
    11341 taaaacaaat ttaaagtcca agtatttatg gagcaaaatt attgcattat aatggcaggc
    11401 ggcattggga gccgtttctg gccttttagc aggaaaaaca ggccaaagca gtttcttgat
    11461 ttttttggta cgggaaggtc tttgcttcaa ttgacttatg accgttttag aaaaataatc
    11521 cccgcagaaa atattttgat tgtctccaac gaactttatc gcgatttgat attggaacag
    11581 cttcccgaaa tcaacgacaa tcaattattg ctcgaaccgg tcagaagaaa tacggcacca
    11641 tgtattgctt acgccattta taaaataaag tcgattaccg aaaacgcaaa tatcattgtt
    11701 tcgccttccg atcatttgat cttaaaagaa tcagaatttt tggagactat acgtcagggg
    11761 ctggaatttg tttccaagaa cgacaatcta ctgacattgg gcataaagcc gagtcgtccc
    11821 gagacaggat acggttacat tcaaatagag tttggagaat caaatttgag aaaagtaaaa
    11881 acttttaccg agaaaccaaa tgcagaatta gccaagttgt tttttgaatc gggtgagttt
    11941 ttttggaatt cgggaatttt tttatggaat ttgaaaacca ttttggcagc cttcgatttt
    12001 catctttccg aaatatcgca aaaattcaag caagggttaa aatatttcaa ttctactgaa
    12061 gaaaaaaaat atattgcaga tatttttcat acctgtccca atatttccat cgattatggt
    12121 atcttggaaa aagcttccaa tgtgtttgtt ctttgttccg attttggatg gtctgatttg
    12181 ggaacttggg gctcgctgta cgacatatcc ccaaaggacg aaaaagagaa tgttaccctt
    12241 aaatgtaaaa ctctttacta cgacagccat cgtaatatcg tggcgttgcc ttcagggaag
    12301 ctggcagtta tacaacaact taatgattat attgttgccg attcggacaa tgtattactt
    12361 atttgcaagc gagaagatga acaacaaatt cgtcagtttg ttaaagatgc cagcattctt
    12421 tacgatggag aattcgattg attttatact ttttaaaact ataaaacatt atgacaccaa
    12481 gcttttttaa catttacaag cctcgtaagt acgactatcg ctacatttat tacgatcctt
    12541 tgaaagaggc aattaaggaa cgtgagaaac aaatggccaa agagaatgga gaacaacctc
    12601 agcaagaata taaacccaat ctgcatcgcg gttcatttcg agaagaagcc aataggatga
    12661 gaggaaaaaa atcaaaagaa ttacgtcgtt cgaacagaaa tgttcttatt attctgatta
    12721 ttttgctgat cattgcctat tatctgttga aataaaatcc aaatgcctgc atgagtgata
    12781 tcatacattt attacctgac tcggttgcca accagattgc agccggtgaa gtaattcaaa
    12841 ggccagcttc tgtgctgaaa gaattaattg aaaattccat agatgcaaat gccaacaaca
    12901 ttattatcca aataaaaaat gcagggcgca ccctgattca ggttacagac aacggaaaag
    12961 gaatgagtga aaccgatgcg aggctggctt tcgaacgtca tgctacctca aaaatatcaa
    13021 atgtttcaga tatttatgcc ttgcaaagtt atgggttcag aggcgaagcg ctggcttcta
    13081 ttgctgcggt ggctcaagtg gaacttcaaa ctcgtcggaa agaagacgaa ttgggaacgt
    13141 tgattgaaat tgaaggttca aaagtaattc gacaggaatc ggttttatgc gaagaaggaa
    13201 caactatcag agtgaaaaat ttatttttca atgtacctgc ccgccgtcgt ttcctcaaaa
    13261 gcgataatgt agaaaagatt catttgctca acgaatttta tcgaattgct ttagtttatc
    13321 ccgatatccg gttttctttt ttcgacgata aagaagaaat tttcaaactc ttaccttctc
    13381 cactcaaact ccgtattgaa aatatttttg gaaaaggcac caaaagaaaa tgggaacaac
    13441 agctgctacc cattgaaacc aataccccat tggttaaaat tgagggatat gtaggtcgcc
    13501 ccgaatttgc cttaaaatcg gcttatcaat atttttttgt caatgctcgc tatatgcggc
    13561 atccttactt tcacaaagca gtgattagtg catacagcca aatgattcgt ccggaggaaa
    13621 atcccaatta ttttatctat ttatatgttg ctccagaaac catcgatgtc aatattcatc
    13681 ctgccaaaac tgaaataaaa tttgaaaatg aacaggctat ctggtctatt cttttggcta
    13741 ctgtccgcga aacgcttggt aaatataatg ttattccatc tatcaacttt aatcaggagg
    13801 atgctatcga ccttcccatt attggcaata acgaaaaaat cgtaccaccc cacactgttt
    13861 tcaatccaaa tttcaatccc tttgaaaaca cattgattaa taaacgacca ccactcaact
    13921 gggaaacgct ttacaaaaat tttgaaaaaa aagaagagag taaaccaact tcctttcaat
    13981 ggatagaaaa cgacattacg gcttatgctt catccgcttc aagtataatt gcagaagaga
    14041 ccctcgacag cagccatttt ttacaactca aaaccaaata tattcttact gctgtaaaat
    14101 caggtttgtt ggtcattcat caacacaagg cgcatgttcg tattctgtac gatctttacc
    14161 tatcacagat caaacatcaa aaaagctctt ctcaacaatt gcttttccct gaaattttag
    14221 aactttctga acatgaaatg ctttattttt catcaataaa aaatgatttg agcaatgtcg
    14281 gttttctatt caaatccgta ggtaaaaacg ctttcgaaat tttcggcata ccaggcccgc
    14341 tagataaaat gaatattcaa gcatttatac atgaattgat agaaaaattc aaagtcaaac
    14401 aatcaatttc cgaggaagat cttcatcaac aaattgcttt atctatggca gaaacatcag
    14461 caatcaaaac cggtcaaaaa cttacacaag aagaaatgac tgacctcgta aatcgtttgt
    14521 ttgccaccga agagccatct ttttccccac aaggtgaaaa aatcttatcc attattccaa
    14581 tggaagaatt tgatagccaa ttttaaaact ttcatttttc cgtttgtttg ctggcaatta
    14641 aagaaaggtt agtatataag ttttcatctg acaatttttt ataataggtg gttccttaaa
    14701 ttcaatagtt tgttaaaaat taaattatct gtcaaataaa tcgagtagaa ataaaattac
    14761 gtggttgtct gaatcaaaat ttattacgca tgaaactgat tgacgaaaaa ctattaaatg
    14821 aacttacccg acaggccaaa gaaaatgccc gcttgcgagt caattacaat ctacatgaac
    14881 atttggaaga taaggttcaa cgtttgctca atgctatgga gccgggtacc aagttaccta
    14941 tccacagaca tcgacatacg gcggaaacat attttttgat acgagggagt atcttagtga
    15001 aatactatga tgacaaggga aatctgacgg aagaaacaga actgaacgta aaggaaggaa
    15061 aatatggcat tcatatacct gtcggtcaat ttcatacact ggaagtgctg gaaagcagta
    15121 ccatcatttt tgaagtaaaa gaagggcctt atcagccaat cggagatgaa gacatactta
    15181 aatcagatat t
//
LOCUS       DAOSCH010000047        14989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2717_length_14989_cov_6.219298, whole genome shotgun sequence.
ACCESSION   DAOSCH010000047 DAOSCH010000000
VERSION     DAOSCH010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14989
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2717_length_14989_cov_6.219298"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(208..2505)
                     /locus_tag="PLZ54_05075"
     CDS             complement(208..2505)
                     /locus_tag="PLZ54_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOJ66336.1"
                     /translation="MKKLYLLSFAFILCFSLSSTAQKVGLVLSGGGAKGVAHIGVIKA
                     LEENGIPIDYIAGTSMGAIVGSMYAMGLTPEEMLNIIKSDDFRFWLTGQNEPEYVFYF
                     RNGNVKPTFMEISFRFNPHDTVRVKSNVLPTHIVPTHQLNYAFMALFSQATAASEQNF
                     DRLFVPFRCVASDIEEKVPVVFSKGSLGDAVRASMTFPLVFKPIVIDNKLLFDGGIYN
                     NFPVDVMRNDFNPDIMIGSVVAKNPQKPTEDNLLLQIENMVMSQTDYVIPKEEGFMLR
                     FDLKNFNMFDFTKADQLYQMGYDSTLQKIEEIKSAISRRISEEELNKNRQDFRDKFPE
                     LKFQNVYIEGIDSLQKLYVEREFHTGNEVFDLKSFKKSYFNLLSDNQISEITPAAVYN
                     KTSGLFDLHLKVKTEDHLKVMMGADISSMASNQAYIGLSYQNLKEYAQTAYVDAQFGR
                     MYNALGVGVRMDAAAKTDFYVKFGFVTHKFDYFDGISLFYNDNRVSYFNQQETYGKLS
                     IGFPLTKKGRIEFGLGGGYLADHYFQNKSLITSGVKNDKSSYSLGSVFVDAESNTLNR
                     PNYPTGGSYYAASLRLTGGNHNEYLNYPVVRELEGQELWLQFLGKYDRYINLSKNVTL
                     GTYGELAISNRKLLPNYTATMIQSPAFRPTLHSTTIFNEGYNANQYAAFGLKPIYSFN
                     NQLHFRTEAYCFVPYKTIYRLPDESAAYSKPFSDVEFISEAAMVLSFKKATLSAFVNY
                     YSAGSNHWNVGFNIGVLLFNKKFLQ"
     gene            2761..3717
                     /gene="fmt"
                     /locus_tag="PLZ54_05080"
     CDS             2761..3717
                     /gene="fmt"
                     /locus_tag="PLZ54_05080"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOJ66337.1"
                     /translation="MRKSKEELRIVFMGTPEFAVESLKILVEGGYNVVGVVTMPDKPA
                     GRGYKVQFSPVKQYALENNLLLLQPEKLKDEIFLENLQSLKADLQIVVAFRMLPEIVW
                     NMPPLGTFNLHASLLPQYRGAAPINWAIINGEKETGVTTFFLTHEIDTGKIILQEKIP
                     ILPSDNAGTLHDKLMMLGASTVKKTVDLIISDAVQPIDQSELLKNTIELRSAPKIFRE
                     NCEIDVSWDVERAHNFVRGLSPYPGAWMNLVFPHTNEKTVLKIFQAEKEFSSHQLPLG
                     SLKTDGKKFAKIALKDGFLVLKEVQAPNKKRMEIGEFLRGLR"
     gene            complement(3722..4099)
                     /locus_tag="PLZ54_05085"
     CDS             complement(3722..4099)
                     /locus_tag="PLZ54_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOJ66338.1"
                     /translation="MKKVIHTSDAPAAIGPYSQAVESSNILFISGQIPICPQEGKIIS
                     TTIDGQTEQVFSNIEAILKSAGYSFGQIVKTTVFLTDMSHFAAMNEIYKRFFTEDCPA
                     RSTIAVKELPQGALVEIETIAMK"
     gene            complement(4505..5311)
                     /gene="tatC"
                     /locus_tag="PLZ54_05090"
     CDS             complement(4505..5311)
                     /gene="tatC"
                     /locus_tag="PLZ54_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOJ66339.1"
                     /translation="MSLNNKANEQETTFWWHLEKLRWVILRTLAVVGLLMVLFFSNKD
                     FLFNKIILAPLNSDFYVYRWFNEIAKLLNFSDLTIDSFHLQLININLAGQFMAHLSIS
                     FAFALIVSVPYLLFEIWRFIFPALYPHERKNIELIFFFSSLLFYLGAIVSYLIIFPLT
                     IRFLGTYQVSSLVPNQIALSSYISALYILVFSLGLTFEIPVLSYFLSRLGIITRQMLK
                     KGRGLALVIILIAAAVITPTTDPFTMMVVTVPLYLLYEVSILVCKKDTGQ"
     gene            complement(5439..5633)
                     /gene="tatA"
                     /locus_tag="PLZ54_05095"
     CDS             complement(5439..5633)
                     /gene="tatA"
                     /locus_tag="PLZ54_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOJ66340.1"
                     /translation="MTLTTILLAIGTPEILIIALVILLLFGGRKIPELMRGLGKGVSQ
                     FKKGMKDVEDEIKEDDNKKE"
     gene            complement(5669..7195)
                     /locus_tag="PLZ54_05100"
     CDS             complement(5669..7195)
                     /locus_tag="PLZ54_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ66341.1"
                     /translation="MLKKLRVVISLLFFIPITLYFLDFRGVLPQQFHWLAKIQLVPAL
                     LSLNLIILTIIAGITFIFGRIYCSSVCPLGIFQDIVSWVSKQINKKKKFKFSTAKNIL
                     RWSVLGITVITFFLGFSFLTGFFDPYSIYGRMATHLFKPVYMEGNNLLAGIFNAAGFH
                     SLYKVQIYLLSIGTFLITLAMMLIIGYLAWKKGRLYCNTICPVGTTLGFLSKYSLFKI
                     RFDQEQCNSCGLCSMNCKSSCIDAKEKTIDYSRCVNCFNCMEACKRNGFKFSTKSALR
                     RKSTDNQTTTDIDNNKRRFLSTLAVSSFAVAGLPQEGIKINGFHKVKRETPVSPPGSK
                     SAENLMSACTSCHLCVSKCPSQIIRPSLFEYGLGGIMQPMLSFEKGFCNYDCTVCSEI
                     CPSGALSALTKEEKHKNQMGQVHFILENCIVYKDETSCGACSEHCPTQAVSMVPYKNE
                     LTIPHTDTGICVGCGACEYACPAFPDKAIYVEGLKVHQQAAIKKEKSNHKIEIEDFGF
                     "
     gene            complement(7202..8140)
                     /locus_tag="PLZ54_05105"
     CDS             complement(7202..8140)
                     /locus_tag="PLZ54_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOJ66342.1"
                     /translation="MDRREFLKMLALSGAAFTIKQSKAMSLMTQHFSGKEINENYDLV
                     AVLGGTPVEMFRKGIAEFGGMEKFVKKGYKVVVKPNIGWDKTPEYAATTNPELVGEII
                     RQCFKAGAKEVTVFDHTCDDWRKCYASSGIEAAAKAAGAKVVPADLESYYESISLPRA
                     KNLKSAKVHKVLLESDVWINVPILKNHGGAKMTISMKNLMGIVWDRGFFHSHDLQQCI
                     ADICTMSKPAVLNVVDAYRLMKTNGPRGKSEADVVLAKGLFMSQDIVAVDTAATNFFN
                     QVRDMPLESVEHLAKAQELKVGTMNLNQLNIKRIKM"
     gene            complement(8166..8564)
                     /locus_tag="PLZ54_05110"
     CDS             complement(8166..8564)
                     /locus_tag="PLZ54_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOJ66343.1"
                     /translation="MEQKTFSEIMQRFADIRFDENFDLIVAIANGGIVPAGIINQKLK
                     TEIQILHINFRDDFQQPKYLTPQLLKPIDFEYTGKKILLVDDRIKTGATINVAKKLLE
                     GAQLIKTFAVNGNADYALYNESCFRFPWII"
     gene            complement(8570..9079)
                     /locus_tag="PLZ54_05115"
     CDS             complement(8570..9079)
                     /locus_tag="PLZ54_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOJ66344.1"
                     /translation="MDTTIKLYHYNYRTLKTYLFATIFIVGNIIFPQLVHSIPNGGLI
                     FLPIYFFTLIGAYKYGFTVGLITAIISPLVNSWIFGMPPVGMLSIIITKSVLLAIGAA
                     YVASKSQKVSLVGLLLAILFYQLTGLMFEWFYTGSLILAYQDLRMGFPGLLLQWLGGY
                     ALLRSIQKL"
     gene            complement(9365..10636)
                     /locus_tag="PLZ54_05120"
     CDS             complement(9365..10636)
                     /locus_tag="PLZ54_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004318741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOJ66345.1"
                     /translation="MQPLAERLRPQTLDQFIGQKHLVGENAILRQMIESGHIASLILW
                     GPPGVGKTTLARIIARQLNCPFYTLSAINSGVKEVREVIDKAKNSRFFSQQSPILFID
                     EIHRFNKSQQDALLGAVENGTITLIGATTENPSFEVITPLLSRCQVYLLTPLDKNDLL
                     ELAQRAIDTDEELKKKKIHLDETEALLRFSGGDARKLLNIIELVVTAEPSETVHITND
                     IVTRRLQQNPMAYDKNGEMHYDIISAFIKSIRGSDPDAAIYWLARMIAGGEDPKFIAR
                     RLVISAAEDIGLANPNALLLANACFDALQKIGWPEGRIILAETTVYLAASQKSNSAYL
                     AIDDALNMVEKTGDLPVPLHLRNAPTKLMKELGYGKDYKYSHDYENHFVAEQFLPDQL
                     KNVSIWKKQDNAAEKKMAEWLEKLWKNRYQK"
     gene            11346..12821
                     /gene="guaB"
                     /locus_tag="PLZ54_05125"
     CDS             11346..12821
                     /gene="guaB"
                     /locus_tag="PLZ54_05125"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005811276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOJ66346.1"
                     /translation="MSFIAERVQFEGLTFDDVLLIPSYSDVLPREVDLSTRFTRHITL
                     KIPIVSAAMDTVTESNMAIAIAREGGIGVIHKNMSIAEQARQVEIVKRAENGMISNPV
                     TILKGATVGNALALMAEFKIGGIPVVDESGYLVGIVTNRDLRFQRDFYKPVEEVMTKE
                     NLITTSRSITLDEASEILQQYKIEKLPVVDDKNKLIGLLTYKDITKAKDKPMASKDKQ
                     GRLRVAAAVGVAADTFERMDALVAAEVDAIVIDTAHGHSKGVIDMLKKARKAYPNLDI
                     IVGNIATAEAARDLAEAGADAVKVGIGPGSICTTRVVAGVGVPQLSAIFEVSKALKDL
                     DVPIIADGGIRYSGDIVKALAAGGETIMAGSLFAGVEESPGETIIFNGRKFKSYRGMG
                     SLEAMEKGSKDRYFQDMEDDIKKLVPEGISGRVPFKGSLYEVVYQLIGGLRAGMGYCG
                     AHNINELHQARFTRITNAGVVESHPHDVTIISEAPNYSRGE"
     gene            12985..14931
                     /locus_tag="PLZ54_05130"
     CDS             12985..14931
                     /locus_tag="PLZ54_05130"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ66347.1"
                     /translation="MKLSLLSLSAALSLSACLFAQTNDPVLMTINGHPVYKSEFEYIY
                     NKNNSNNSLDKKSLDEYVDLFINFKLKVEEAKAQGIDTTSAFINELAGYRSQLAKPYL
                     TDQKFEEKLMQEAYQRLKEDVEVSHILIRIPQNATPADTLAAWNKVKSILKRLEKEDF
                     AKVAKEVSEDETAEKTGGYIGWITAFQTFYPFETMAYNTPVGGISQPVRSIYGYHIIK
                     VHNRRNSVGEVQVAHIMRFTSPNDSVKNKRAKQVIDSLYQCVKNGEDFGALASKYSED
                     KPSAARNGELPWFGTGRMVPQFEAAAFSLKKVGDVSEPIQTPFGWHIIKLLGKKDLPS
                     FNDMKSDLERRIKQDERANLAQQSFVNQLKKEYNFRLVDENVQDFYKLLKGRTLNDSV
                     FLTEIGKLNKPLFTFADKQYTQHDFANFLQKNQFTQKSIPTEVIDEKLNQFINAELMA
                     YEDTQLEKKYDDFRFLMQEYHDGILLFEVSNREVWDKALRDTVGLASYFEQHKSDYKW
                     VKPHYKGRVIYCKDKNTFKTAKLIAKRLANDSIDKYLTSRLNSDSVQYVKIEKGLFVE
                     GDNKAVDKLVFNKKEKFTPDADYPYVFVVGKLLKETPEDYTDVRGAVIADYQDYLEKE
                     WIKNLRSKYSFSVDKNVLRTVKEN"
BASE COUNT         4715 a         2746 c         2885 g         4643 t
ORIGIN      
        1 cgcaatatcc aataaaaaag agactgtctc tacttttgag acagcctctt ttttatattt
       61 gtcccgaaaa ataaatttac tttctcttcc ctattggaat aggtatttcc attattttaa
      121 tctacttcta aaaagatatt tcatcatttt ttcggaaaga tgaaattaat tttttgctct
      181 ttgcctacaa atcattacac tcagatttta ttgcagaaat tttttgttaa aaagaagaac
      241 gccaatatta aaacctacat tccaatgatt cgaaccagcg ctgtaataat tcacgaatgc
      301 agaaagtgtt gcttttttga agcttaaaac catagcagct tccgaaataa attccacgtc
      361 ggaaaaaggt ttggaatatg ctgccgattc atcaggaaga cgataaatgg ttttatatgg
      421 aacaaaacaa taggcttcgg tcctaaaatg caattgattg ttgaaactgt aaatgggttt
      481 cagtccgaat gctgcgtatt gattggcgtt gtaaccctcg ttgaaaatgg tagtactgtg
      541 caacgtcgga cggaatgcag gcgactgaat catagtagcc gtataattgg gtagcaattt
      601 tcggttagaa atggccagtt ccccataagt tcccaatgta acatttttcg aaaggtttat
      661 atatcgatca tattttccaa gaaactgcag ccacaactct tgcccttcga gttccctcac
      721 aacaggataa ttaagatatt cattgtgatt accaccggtc aaacgtaacg aagcagcata
      781 ataagatccg ccagtaggat aatttggacg atttagcgtg ttactttcgg catcaacaaa
      841 aacgcttccc aatgaataac tgcttttgtc gttttttact cccgaagtaa tcaagctttt
      901 gttctgaaaa taatgatcgg ctaaataacc tcctcccagc ccaaattcta ttctaccttt
      961 ttttgtcaga ggaaatccaa tgctcaattt accgtatgtt tcctgctgat tgaaatacga
     1021 cacgcgatta tcgttataaa acaagctgat tccatcaaaa taatcaaact tatgcgttac
     1081 aaagccaaac ttcacataaa aatcggtttt ggctgccgca tccattctaa caccaactcc
     1141 cagcgcattg tacattctac caaactgagc atccacataa gccgtttgcg catattcctt
     1201 caaattttga tatgaaagtc cgatatatgc ctgattgctt gccatagatg aaatgtcggc
     1261 tcccatcata accttcaaat gatcttcggt tttgactttc aaatgtaaat caaataaacc
     1321 ggaagtttta ttgtacaccg ctgcaggcgt gatttccgaa atctgattat cggaaaggag
     1381 attgaaataa ctttttttga aacttttcaa atcgaaaact tcattcccag tatgaaattc
     1441 gcgttccaca taaagttttt gcaaagaatc tattccttct atataaacat tttggaattt
     1501 aagttcagga aatttatccc gaaagtcctg tcggttttta tttagttctt cttctgaaat
     1561 tcttcttgaa atggccgatt ttatctcctc aattttctga agtgtggaat cgtaacccat
     1621 ttgataaagc tgatcagctt ttgtaaaatc aaacatgtta aaattcttta aatcgaaacg
     1681 aagcataaag ccttcttctt ttggaataac ataatcggtt tgagacatca ccatattttc
     1741 aatctgtaaa agaagattgt cttcagtggg tttttgcgga ttttttgcca caacgctccc
     1801 aatcataata tcgggattaa agtcattccg catcacatct acagggaaat tattgtaaat
     1861 acctccatca aaaagcaatt tgttgtctat tacgatgggt ttaaaaacca atggaaaagt
     1921 catggaagcc cttactgcat cgcccaatga gcccttgctg aaaactaccg gaactttttc
     1981 ctcaatgtcg gaagctacac aacgaaacgg gacaaaaagt cgatcgaaat tctgttcgga
     2041 agcggctgtt gcctgtgaaa ataaagccat aaaagcataa ttgagttgat gggtaggaac
     2101 aatatgcgta gggaggacat tgcttttaac cctcactgta tcatgtggat tgaaacgaaa
     2161 actgatttcc ataaatgttg gctttacatt accattacga aaatagaaaa catattcggg
     2221 ttcattctgt ccggtaagcc agaatctaaa atcgtcggac ttgataatat tcaacatttc
     2281 ctctggtgta agtcccattg catacatgct gccaactatc gcccccatag aagttcctgc
     2341 tatgtaatcg attggaatac cgttttcttc caacgcttta ataactccaa tatgggcaac
     2401 gcctttggct ccgccaccac tcaatactaa cccaactttt tgagcggtag aacttaaaga
     2461 aaaacataaa ataaatgcga aggataataa atataatttc ttcattaaaa tcgagattta
     2521 caggataaag ttacaaaaaa aaattgagtt aatgataatt tttgcttaaa aatttgggtt
     2581 ccctattaaa accttttttt ttcgaaaaga gtgaaaaaat tagatttttt gaagataaac
     2641 tgtaaaattt ctccacgttg ttaatgcttt tattttttga tgaccgtagc atgaaaattt
     2701 ttttattatt tttgtgttga gaccgaatta aaaattgctg caatatttta aaaaaatgac
     2761 atgagaaaat caaaggaaga gcttcgtatt gtttttatgg ggacacccga gtttgcggtt
     2821 gaaagtttaa aaattcttgt cgaaggcgga tacaatgtcg taggagtagt tactatgccc
     2881 gacaagcctg caggacgtgg atacaaggtt cagttttcgc ctgtaaaaca gtatgcactt
     2941 gaaaataatc tgttacttct tcagccggaa aaactcaaag atgaaatttt tctggaaaat
     3001 ctacaatctc tgaaagcaga tttgcaaata gttgttgctt tccgtatgtt gccggagata
     3061 gtgtggaaca tgcctccatt gggaactttt aatcttcatg cttccttgtt gccgcaatat
     3121 cgaggtgccg cacccatcaa ctgggcgatt atcaatggcg aaaaggaaac cggggtaacg
     3181 actttttttc tcacccacga aattgatacg ggaaaaatta ttcttcagga aaaaattcct
     3241 attcttcctt ccgataatgc cggcacttta catgacaagt tgatgatgct tggggcttcc
     3301 accgttaaaa aaactgttga tctgataatt tctgatgcag tgcaacccat tgatcagtcg
     3361 gaattgttga aaaatacgat tgagttgaga tcggcaccca aaattttcag ggaaaattgc
     3421 gaaatcgacg tttcgtggga tgttgaacgc gcgcataatt ttgtacgcgg actctctccg
     3481 tatcctggag catggatgaa cttagttttt ccgcatacaa acgaaaaaac ggttttgaaa
     3541 atttttcagg cagagaaaga attttcttct catcagttgc ctttgggaag tttgaaaacc
     3601 gatgggaaaa agtttgctaa aattgcgttg aaagatggtt ttctggtgtt aaaagaagta
     3661 caggccccca acaaaaaacg aatggaaatt ggcgaatttc ttcgcggcct gcgataaaaa
     3721 tttatttcat ggctatcgtt tctatttcaa ccaaagctcc ctgaggaagt tccttaacgg
     3781 caatcgttga gcgggccgga caatcttccg taaaaaatct tttgtaaatt tcgttcatgg
     3841 ctgcaaaatg actcatatcg gtcaggaaaa ccgtagtctt aactatttgc ccgaacgaat
     3901 atcctgccga tttcaaaatg gcttcgatat tggaaaaaac ctgctcggtt tgtccgtcaa
     3961 tggtagttga aataattttt ccttcttgtg ggcaaattgg aatttgaccc gaaataaaaa
     4021 gaatgttact gctttctact gcttggctgt aaggtccaat agctgcgggc gcatcagagg
     4081 tatgaataac ttttttcata attagtagtg ttagagcatt caagtaagga atgcaacttt
     4141 ttgtttttac aaatttcgca ataaaaattc atttattgtt aagaaattaa ttttttcttg
     4201 gtctgttgtt ttaaaatatc ttctattgaa tttataacag aactcattta tattgattac
     4261 caaaattttc gttagtaagt tgtgaataca tccaacacca acaacttaac cttgccaaaa
     4321 ttaatatgta acccgaatca atatcttttg cagattttgt tgcctttaca atttgtactt
     4381 aatgcgattt gacatgtttt gatagttttt aaaaaggagt atcatcatcg gttgtcaatt
     4441 cgactaatgt atctatgcat ttctttacag tatttgaaac gttatcagct ccgaaactat
     4501 tacgttattg tcccgtgtct tttttacaaa ccaaaatact gacctcatag agtaaataaa
     4561 gcggaacagt tacaaccatc atggtaaaag gatcggttgt aggagtaata actgcggcag
     4621 ctattaaaat aatcacaagt gcaagtcctc gtcctttttt caacatttgc cgagtaatta
     4681 ttcccaatcg agataaaaag tacgagagaa caggtatttc aaaagtaagt cccaatgaaa
     4741 agacgagaat atacaaggcg ctgatgtaag aagagagagc aatctgattg ggtaccagcg
     4801 aagaaacctg ataggttccc aaaaatcgga tggtcaacgg aaaaataatc agataactga
     4861 cgattgcacc cagataaaac aacaaagaag aaaaaaagaa aattaattct atattttttc
     4921 gttcatgcgg atacaaagca gggaagataa aacgccaaat ttcgaatagc aggtatggaa
     4981 ccgagacaat gagggcaaaa gcaaacgaaa tagacaaatg ggccatgaat tgccctgcca
     5041 gattgatatt gataagctga agatgaaaac tgtctattgt caaatcgctg aaatttaaca
     5101 acttcgcaat ttcgttaaac cagcgataga cataaaaatc agaatttaag ggtgctaaaa
     5161 taattttgtt aaacagaaaa tctttgttgg aaaagaataa caccattaac agtcctacaa
     5221 ctgccaatgt tcgtaaaatg acccagcgaa gtttttccaa atgccaccag aaagtagttt
     5281 cctgttcatt tgccttattg tttaacgaca taccgaattg ttatcgttat gttatatgga
     5341 tgtcatttgc aaatcagttc tatccatata ttaattttgg tatttttact caaaaaaatt
     5401 gtttgggaaa agaatgaata acttgaaata taagataact attccttttt gttgtcatct
     5461 tccttaattt catcttctac atctttcatt ccttttttaa actggctgac tccttttccc
     5521 aatccgcgca tcaattctgg aatttttctt cctccaaaaa gcagtagaat aactaaggca
     5581 atgattaaaa tttcgggtgt tccgatagct aataaaattg ttgtaagtgt catgtcgata
     5641 ataaattaat agttcaaaaa ttttattctt aaaaaccaaa atcttctatt tcaattttgt
     5701 ggtttgattt ttcttttttt atggcagctt gctgatgaac tttgagtcct tccacataaa
     5761 ttgctttgtc gggaaaggcg ggacaagcat attcacaagc gccgcaacca acgcaaatac
     5821 cggtatcggt atgtggaatg gtcaattcat ttttgtaggg aaccatgctt accgcttgag
     5881 tcggacaatg ttccgaacag gcaccacaac ttgtttcatc tttatataca atgcagtttt
     5941 ccaaaataaa atgcacctgt cccatctgat ttttgtgttt ctcttctttg gtcaaagctg
     6001 agagagctcc actgggacaa atttcggagc aaacagtaca atcataatta caaaatcctt
     6061 tttcgaaaga aagcattggc tgcatgatgc ctcccaaacc atattcaaaa agtgatggcc
     6121 gaatgatttg tgatgggcac ttgctgacgc aaagatgaca cgatgtgcag gcactcatta
     6181 aattttcggc actttttgat ccgggaggac taacaggagt ttctcttttt accttgtgaa
     6241 aaccatttat tttgattcct tcctgaggca atccagctac ggcaaaagac gaaacagcca
     6301 gtgtgctcag aaaacgtcgt ttgttattat caatatccgt tgtggtctga ttgtctgtgc
     6361 tttttcttcg taaagcagat ttggtggaaa atttaaaacc gtttcgtttg caggcttcca
     6421 tacaattgaa gcaatttacg caacgcgagt aatcgattgt tttttctttt gcatcgatac
     6481 aggaagattt gcagttcatg ctgcaaagtc cgcaggaatt gcactgttct tgatcgaatc
     6541 tgattttaaa aagggaatat ttgctgagaa atcccaatgt ggttccaacc ggacaaatgg
     6601 tattgcagta aagacgtccc tttttccatg ccaaataacc aattatcagc atcatagcca
     6661 aagtaatcag aaaagtgccg atgctgagca ggtaaatctg aactttgtag agagaatgaa
     6721 atcccgcagc attaaaaatt ccagccagca aattgtttcc ttccatatac accggtttga
     6781 acaaatgggt ggccatacgt ccataaatgc tgtaagggtc gaaaaaaccg gttagaaaag
     6841 aaaaacccag aaaaaaagta ataactgtaa ttcccaaaac tgaccaacgg agaatgtttt
     6901 ttgctgtact gaatttgaat tttttctttt tattgatctg tttggaaacc catgaaacaa
     6961 tatcctgaaa aatgcccagt ggacagacag atgagcaata aatccgtcca aaaatgaaag
     7021 taattccggc tattatcgtc agaatgatga gattaagcga gagcaatgcc ggtaccagct
     7081 gaatttttgc cagccaatga aactgttgtg gcaaaacacc tcgaaaatca agaaaatata
     7141 aagtaatcgg gatgaaaaac aggagagaaa tgacgactct taatttcttc aacatctctg
     7201 tttacatttt aatccgtttt atatttaact gattgaggtt catggttcca actttcaatt
     7261 cctgagcctt tgccaaatgt tcgacgcttt ccagtggcat gtctctcacc tgattgaaaa
     7321 aattggtggc agccgtatca acagctacaa tatcctgcga catgaacaat ccttttgcaa
     7381 gcacgacgtc tgcttcggat tttcctcgtg gaccattggt tttcatcagt cggtaggcat
     7441 caaccacatt caaaaccgcc ggtttactca tggtacaaat atcggcaatg cattgttgta
     7501 aatcgtggct gtggaagaaa cctctgtccc aaacaattcc catgaggttt ttcatggaaa
     7561 tggtcatttt tgccccgcca tgatttttca gaatgggaac attaatccac acatccgatt
     7621 ccaaaagaac tttgtgaact ttggccgatt tcagattttt ggcacgaggg agtgaaatgg
     7681 attcataata ggattccaaa tcggctggaa ctaccttcgc tcctgcagct ttggctgctg
     7741 cttcaattcc gctgctggca tagcattttc tccagtcgtc gcaagtatga tcaaaaacgg
     7801 taacttcttt ggcgccggcc ttgaaacatt gccgaataat ttctcctacc aattcaggat
     7861 tcgttgtagc agcatattct ggagttttat cccagccaat atttggtttt acaacaacct
     7921 tatatccttt tttgacaaat ttttccattc cgccaaattc ggcaattcct tttcggaaca
     7981 tttcaaccgg cgttcctccc aacacggcaa caagatcgta attttcgttt atttcttttc
     8041 cgctgaaatg ctgagtcatc agtgacatgg ctttgctttg ctttatggta aaagcagctc
     8101 cactgagtgc aagcattttt aaaaattctc ttctgtccat agtgttgttt gtttaaaaac
     8161 aaatttcaaa ttatccaggg aaaacggaaa cagctttcgt tgtaaagagc gtaatctgca
     8221 ttcccgttaa cggcaaaggt tttgataagt tgtgctcctt ccagcagctt ttttgcaacg
     8281 ttaattgttg caccggtttt aattcgatcg tccaccaaca aaattttttt ccccgtatat
     8341 tcaaaatcaa tgggttttaa tagttgggga gttaaatatt ttggttgttg aaagtcatca
     8401 cgaaaattga tgtgcaaaat ttggatttct gtttttaact tctgattgat aatacctgca
     8461 gggacaattc caccgttggc aatagcaaca atcaggtcga aattttcatc aaatcggata
     8521 tcggcaaaac gttgcatgat ttccgaaaaa gttttctgtt ccattcaaat tacagctttt
     8581 gaatggatct caacaaggca taaccgccca accactgcag aagcaatccc ggaaatccca
     8641 ttctcaaatc ctgataggca agaataaggc tgccggtgta gaaccattca aacatcagcc
     8701 cggttaactg ataaaacaga attgccaaca acaatccaac aagactcact ttttgacttt
     8761 ttgaagcaac ataggcggct ccaattgcca ataaaacaga tttagtaata atgatagaca
     8821 acatgccaac cggtggcatt ccaaaaatcc aactgttgac caaaggcgaa attattgccg
     8881 taataagtcc aacagtaaag ccgtatttgt aagctcctat tagagtaaaa aagtaaatgg
     8941 gcaggaaaat aaggccgcca ttgggaatgc tgtgtaccaa ctggggaaaa attatattcc
     9001 cgacaataaa aatagttgca aataaatatg tttttaatgt tcgataattg tagtgataaa
     9061 gtttaattgt ggtatccata gcaaatcatc attattaaag aattaatttt caatcttagc
     9121 ataagacaaa aatataaata aaaaacaaat cattctccaa aattaagcaa attttacagt
     9181 cattttaaat tgaaaatgaa aattataatg ttattcgctc gctcccgccg atttgtaatc
     9241 ggtggtttgg tcttgttcgg cgtatcctcc tggctacgtc gcactaaaaa taaaatctcc
     9301 ggttttttta atggtgaatg attttgagag gatttgcttt tttacttgag agaaagaaat
     9361 ttttttattt ttggtatctg tttttccaaa gcttttccaa ccattcggcc atttttttct
     9421 ctgcagcatt atcctgtttt ttccaaatgc tgacattttt aagctggtcg ggaagaaact
     9481 gctctgctac aaaatgattt tcgtaatcgt gtgaatattt gtaatctttt ccgtaaccaa
     9541 gttctttcat gagtttggta ggtgcatttc ttaaatggag tggaactggc agatctccgg
     9601 ttttttctac catattcaaa gcatcgtcaa tagccagata ggccgaattg cttttttgag
     9661 aagctgccag ataaactgta gtttcggcga gaataatcct tccttcgggc catcctattt
     9721 tttgcagtgc atcaaaacag gcgtttgcca gcaataaagc attgggattg gcaagcccaa
     9781 tatcttcagc agcagaaatg accaatcttc gagcaataaa tttcggatct tcaccgcccg
     9841 ctatcattcg ggcaagccaa taaatggcgg catcagggtc gctgccacga atggatttga
     9901 taaacgcaga aataatatcg taatgcattt ctccattttt atcgtaagcc attgggtttt
     9961 gctgcagtcg gcgggtaaca atatcattgg taatgtgaac cgtttcggaa ggttcggcag
    10021 taacaaccag ttctatgata ttcagtaatt tgcgggcatc acctcccgaa aaacgcagca
    10081 aagcttccgt ttcatccaaa tgaatttttt tctttttcag ttcttcatcg gtatcaatgg
    10141 ctctttgagc caattccaac aaatcgtttt tatccaatgg agtgagtaga taaacctgac
    10201 agcgggagag gagaggcgta attacttcaa aggaaggatt ttctgtggta gcaccaatta
    10261 aagttatagt gccattttct acggctccaa gtaaagcatc ctgttgcgat ttgttgaaac
    10321 gatgaatttc gtcgataaac aaaataggac tttgttgcga aaaaaaacga ctgttcttgg
    10381 ccttgtcgat tacttctctg acttctttca ctcctgaatt gatggcactt agcgtataaa
    10441 agggacaatt cagttgtcgg gcaatgattc ttgccaacgt tgtctttccc acgccgggcg
    10501 ggccccataa aatcaaggaa gcaatatgcc ccgattctat catctgacgc aaaatggcat
    10561 tttcgccgac caaatgtttt tgtccgataa attggtcaag tgtttgtgga cgaagtcgtt
    10621 ctgctaaagg ctgcatgaaa atttgttttg aattactatt acaaatttag ttcttaattt
    10681 tgattttttt gatttttcag ttgaaaaatt catgaagttt atagtttttg agttagcaat
    10741 ttatgagatt caaggttgcg agtttcgagt agaaatattt ttgaaaaaag ttcggcgcag
    10801 cctcaaagct acgtcgaatt aaaaataaaa tctccagatt ttcaataagc gaataaggtt
    10861 ttggatggta ggaggatttg cttgttatga aggttttttt gaaaaataag gtttaaagaa
    10921 ttgaccttgc ctggtgagcc gaaccaacgg tcagcgtagc taaaaatcag tgaagttggc
    10981 gttaaaaatc ttttctgttt gagcgacgga ggagcgagtt tagaagattt tagccaacga
    11041 acgtcaattt ttcttgtaag tggggtttgg ggcaatgccg cgttattaaa tatctgaaaa
    11101 taaataatgt aaatatttgt acaatttttt gtcatgtact aagtaattca ttaccgaaaa
    11161 ttttttcaat caaatcttta aatttcggct tatatagaac ccgaagatgg ggtatcatat
    11221 tagtttttca aatgattttt tccatagtaa caaataaata tgtaaatttg catgcaataa
    11281 tttcataggt tatatgaata tatattattt atctcattaa aaatcaatta aataaattta
    11341 tttctatgtc atttattgca gaaagagttc aatttgaagg tttaacattt gacgatgttt
    11401 tgcttattcc atcatattcc gatgttttac ctcgtgaagt tgacttatcc acacgtttta
    11461 ctcgccatat cactttgaaa atccccatag tttcggcagc tatggatacg gtaacagaat
    11521 caaatatggc tatcgccata gccagagaag gtggaatagg agtgatccac aaaaatatgt
    11581 ccattgctga gcaggcacga caggttgaaa ttgttaaacg cgcagaaaat ggaatgattt
    11641 ccaatccggt aactatttta aaaggtgcta ctgttggcaa tgcgctggct ttgatggccg
    11701 aatttaaaat tggtggtatt cctgttgtag atgaatcagg ttatttggtg ggaatagtga
    11761 ccaatcgcga tttgcgtttt cagcgcgatt tttataaacc tgtggaagaa gttatgacga
    11821 aggaaaatct gattacgact tcccgttcca ttactttgga tgaagcttcc gaaattttgc
    11881 aacaatacaa aatcgaaaaa ttacctgttg tcgatgacaa aaacaagctg attggtctgt
    11941 tgacctataa agatattacg aaagccaaag ataaaccgat ggcctcgaaa gacaaacagg
    12001 gacgtttaag agttgctgct gcagtgggag ttgcagccga tacttttgag cgtatggatg
    12061 cattggttgc tgcagaagtg gatgctattg ttattgatac tgctcatggg cactcaaaag
    12121 gggttatcga tatgttgaaa aaagcacgca aagcatatcc caatcttgat attatagttg
    12181 gaaatatagc aacagctgaa gcagctcgcg atttggccga agccggagca gatgccgtta
    12241 aagttggtat tggtccaggt tctatttgta caacacgcgt agtagctgga gttggcgttc
    12301 cacagttgtc tgcaattttt gaagtttcta aagcgctcaa agatttggat gtccccatta
    12361 ttgcagatgg cggcattcgt tattccggcg acattgttaa agccttagca gcgggaggag
    12421 agacgatcat ggcaggttca ctttttgccg gagttgaaga atcaccggga gaaaccatta
    12481 tttttaatgg acggaaattt aagtcctatc gtggaatggg ttcgctcgaa gcgatggaaa
    12541 aaggttccaa agaccgctat ttccaggata tggaagacga tattaaaaag cttgttccgg
    12601 aaggaatctc cggtcgtgta ccatttaaag gttctttata cgaagttgtt tatcagttaa
    12661 ttggcgggtt gcgcgcaggc atgggttatt gcggagcgca taacattaac gaattgcatc
    12721 aggccagatt taccagaatt accaatgcgg gtgtagtaga aagtcatcct catgatgtta
    12781 ccattatcag cgaagcgccg aattacagca gaggcgaata aaagcttaaa attcaaaaaa
    12841 aatcaaattt ttacaacatt atcttttttg tttcgttaat ttttttgcag tgcgatgaag
    12901 attttgcttt ctaaagctga taaagcaagt ttttaagcac aatgtttcaa attagtgagt
    12961 aaaaatatta atcgtttttt atctatgaaa ttatcccttc tttctctatc ggcagcattg
    13021 tcgctttcgg catgtttgtt tgcacagacc aatgatccgg tattgatgac cattaatggg
    13081 catccggtct ataaatcgga atttgaatac atttacaaca aaaacaattc caacaattct
    13141 cttgataaaa aatcattgga tgaatatgtg gatttgttta ttaatttcaa attgaaagtt
    13201 gaggaagcaa aagctcaggg aattgataca acttctgctt ttataaatga gttggcaggt
    13261 tatcgttcac aattggcaaa accttatctg accgatcaaa agtttgagga gaaattgatg
    13321 caggaagctt atcagcgact gaaagaggat gtggaagtga gtcatattct cattcggatt
    13381 ccgcaaaatg caactccagc cgatactttg gcagcttgga acaaagtaaa gtctatttta
    13441 aaacgtttgg aaaaagaaga ttttgccaaa gtagccaaag aagtatctga agatgaaaca
    13501 gcggagaaaa ccggtggtta tattggctgg attacagcat tccagacttt ttatcctttt
    13561 gaaacaatgg cttacaacac tcctgtgggt ggaatttcac aacctgttcg ttccatttat
    13621 ggctatcata ttataaaggt tcacaacaga agaaattcgg tcggtgaagt tcaggttgca
    13681 cacatcatgc gatttacttc tcccaatgac agtgtaaaaa acaaacgtgc caaacaggtt
    13741 atcgattcgt tgtatcagtg cgtgaaaaat ggcgaagatt ttggagcttt ggcaagcaaa
    13801 tattctgaag ataaaccttc tgcagcccga aacggtgaat taccctggtt tggaacgggg
    13861 cgaatggttc cacaatttga agctgctgct ttttcattga aaaaagtggg cgatgtttcc
    13921 gaaccaatac aaacgccttt cggctggcat attattaaac tactcggaaa aaaggattta
    13981 ccttctttca atgatatgaa atctgatttg gaaagacgaa tcaaacagga tgaacgggca
    14041 aatcttgctc agcagtcgtt tgtcaatcaa ctaaaaaaag aatataattt ccgtttagtg
    14101 gatgaaaatg tacaagattt ttataaatta ttgaaaggga gaacattaaa tgattccgta
    14161 tttttgactg aaatcgggaa attaaataaa ccattattta cctttgccga caaacagtac
    14221 acccagcacg attttgcaaa tttccttcag aagaatcagt ttactcaaaa aagcattccg
    14281 acagaagtaa ttgacgaaaa gctgaatcag tttattaatg cagaattaat ggcttatgaa
    14341 gatactcaat tggagaagaa atatgacgat ttcagatttc tcatgcaaga atatcacgac
    14401 ggcattctgt tgtttgaagt tagcaatcga gaagtttggg ataaagcgtt gagagatact
    14461 gttggtttgg cttcctattt tgagcagcac aagtcagatt ataagtgggt aaaacctcac
    14521 tataaagggc gtgtgattta ttgcaaggac aaaaacactt tcaaaaccgc taaactgatt
    14581 gccaaaagat tggccaacga ttccatagac aaatatttga ccagccggct gaatagcgac
    14641 tctgttcagt atgtaaaaat tgagaagggg ctttttgtgg agggagacaa caaggcagtc
    14701 gacaagctgg tattcaataa aaaggaaaag tttactcctg atgccgatta tccatacgtc
    14761 tttgtagtcg gtaaattgtt aaaggaaacg cctgaagatt atactgatgt cagaggagct
    14821 gtaattgccg attatcagga ctaccttgaa aaagaatgga taaaaaatct tcgttcaaaa
    14881 tatagttttt cggttgataa aaatgttctt agaaccgtta aggaaaacta ataatttttg
    14941 acaggcgacg ggaaattgct aattttatct ttccaatttc gagaaatcg
//
LOCUS       DAOSCH010000048        14808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2748_length_14808_cov_6.251203, whole genome shotgun sequence.
ACCESSION   DAOSCH010000048 DAOSCH010000000
VERSION     DAOSCH010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14808
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2748_length_14808_cov_6.251203"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..1437)
                     /locus_tag="PLZ54_05135"
     CDS             complement(199..1437)
                     /locus_tag="PLZ54_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOJ66348.1"
                     /translation="MEPKHLYFPSADIHLDILLPASKSISNRALIINALSGSRSEIRN
                     LSDCDDTKVMLRALKLDQSIIDVGAAGTAMRFLTAYLAMTEEEWTLTGSERMKQRPVR
                     LLVEALNDLGAEIEYLEKSGFPPLSIKGKKLTGGTIHFDGSVSSQYISALMMTAPYMN
                     EGLNIILEGEIISLPYILMTKNMMKEFGVEVNFLTNRIEIAPQFYKPVDFTVESDWSA
                     ASYWFEILSIAGEGEIFLKGLHQNSYQGDSKVAELFENLGVAVQYFSDGVLLTPNRKK
                     IDFFNYDFINQPDLAQTFAVTCCLKNIPFHLKGLKSLKIKETDRISALKTELAKFGFL
                     LTEPAEGELSWSGEKKDACHFEETGEISIKTYDDHRMAMAFAPAALLFPIAIEHPEVV
                     SKSYPTFWEDVEKIKKGKVN"
     gene            complement(1546..2712)
                     /locus_tag="PLZ54_05140"
     CDS             complement(1546..2712)
                     /locus_tag="PLZ54_05140"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOJ66349.1"
                     /translation="MNNFNFYSPTYFVFGKGRENEAGKYVKRFGGTKVLLHFGGNSAI
                     KSGLLDRVKKSLTQEGISFVELGGVLPNPRSGLVYKGIEICKNEGVDFILAVGGGSVI
                     DSAKAIAAGALYDGDFWDFYTGKKVEKALPAGTILTIAAAGSEGSSSSVITHENGLLK
                     RSAEGNALRPVFSILNPELTFTLPAYQTACGITDMMAHVMERYFTNTREVEITDRLCE
                     GILLTIIKEAPVAIAEPTNYGARANIMWAGMVAHNDICGVGREQDWATHQMEHELSAL
                     YDVAHGAGLAVMFPAWMKYVMNHDIMRFAQFAVRVWGCEMDFQHPEITAKEGIAKFEQ
                     FLISIGMPIRFAQLGAKAEDIPTLVKNMRLGNCTVGSFVKLTEKDVAEIYKLAV"
     gene            complement(2742..4730)
                     /locus_tag="PLZ54_05145"
     CDS             complement(2742..4730)
                     /locus_tag="PLZ54_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4954 family protein"
                     /protein_id="HOJ66350.1"
                     /translation="MKNYRLLTENEIHLLTSKGCTAENWEMVRVSEGFKPDYIAEVHF
                     SGNIFLGNFNKIFVLAGGLQRHAGIRRCCLHNCTIEDDVYIDQVANYIANYHIGEGSY
                     IENVNLLVVEKDSTFGNGVKVPVMNEGGGREIPIFDFLSAPLAYVLTLYRHRPVLIQK
                     IQKLIDDYACKLKSDTGTIGKNVRIVNAGSIKNVRIGDCANIEGASCLINGSINSNAF
                     APVTIGAGVKCDDFIISSGVSVTDSTLISRCFIGQGCILGKHYSVLDSLFFSNCQGMH
                     GEATAIFAGPYTVSHHKSTLLIAGMFSFLNAGSGSNQSNHMYKLGPIHQGLAERGAKT
                     TSDSYLLWPAKIGAFTLVMGRHTKHSDTSDLPFSYLIENATDSFLVPAVNLRSVGTIR
                     DAQKWPKRDNRKDPKKLDPINFNLLSPYTIQKMMKGVEVLKNLQKISGETTEIYTYQN
                     CSIKNSSLLRGIDLYHIGIYKFLGNSLISRLKDKHFNTIEDIREILKPDTSVGSGEWI
                     DLSGLIAPKTEIEQLMKKIEDQPITLEDIQSEFEKMHEAYYNYEWTWAKEKLEIYWRK
                     PIAEVTREDLIQMVELWKNSVVTLDHLIYDDAKKEFSLNAKTGFGVDGDEQQKSLDFE
                     SVRGSFENNPFVLEVINHIKIKSELGDELLDRLKKVIN"
     gene            complement(4802..5983)
                     /gene="hflX"
                     /locus_tag="PLZ54_05150"
     CDS             complement(4802..5983)
                     /gene="hflX"
                     /locus_tag="PLZ54_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOJ66351.1"
                     /translation="METNNYQEKTILVGLVTPQQNREKAKEYIEELAFLAKTAGAVPE
                     KIFLQQLDHPNPETFVGPGKLEQIKNYLADHPVDMAVFDDELSARQIRNLESELKIKI
                     LDRTNLILDIFAKRAKTASAKTQVELAQYQYLLPRLTRMWTHLERQQGGIGTRGPGET
                     QIETDRRIILSKIAQLKQNLKEIDRQMSTQRKNRGKLVRVALVGYTNVGKSTLMNLLS
                     KSNIFAEDKLFATLDTTVRKVIIDNLPFLLTDTVGFIRKLPHHLIESFKSTLDEVREA
                     DLLIHVVDVSHPNFEEQIQVVNQTLKEITNEEKPMILVFNKIDAYNHKAKDPDDLTPK
                     KPENISLEEIQKTWMAKIHEDCVFISATKKQNIEELKKIMYQKIKAIHVQRYPYNDFL
                     Y"
     gene            complement(6098..6793)
                     /locus_tag="PLZ54_05155"
     CDS             complement(6098..6793)
                     /locus_tag="PLZ54_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOJ66352.1"
                     /translation="MEEKKSPKASLENKKSLFILIGLVMALSFIYIALEWTAREITIY
                     EGVQTDLKTEEELDIIQTTQDETPPPPPPPAPEVIEVLNVVENTVETQTINIETEDTK
                     NKAVEIIAAPVAAPMAAPIHEEDEQVIFQVVETMPQFPGGEQALFKFLNENIKYPVIA
                     QENGIQGRVICQFVVNTDGSIVDIQVVRGVDPSLDKEAVRVIQSMPKWIPGKQRGKPV
                     RVRFTLPINFKLQ"
     gene            7087..8106
                     /gene="porQ"
                     /locus_tag="PLZ54_05160"
     CDS             7087..8106
                     /gene="porQ"
                     /locus_tag="PLZ54_05160"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOJ66353.1"
                     /translation="MIKRIVLFLWFLITFVALVYPQAGKGVYQFLDLPVSSRIAAMGG
                     TNVSLKDNDINFSFCNPSLLTDKTNDFLSLSMANYLADIQYGSAVFGKNFKNKHYMAF
                     GMQYVDYGTFQETTEQNEIIGEFTAKDMALYIMYAYPITEKISVGGTFKPIYSAYERY
                     SSFGAAIDAGISYNDPTNFFSAGLVLRNIGSQIKGYYSDEEGQHYEPLPFNIQLGITK
                     KLAYAPLRFTGTLHNLQHWDLSYASSTQNETKKINFFDMALRHFIFNAEFIPSNNFYL
                     TLGYNFRRAKEMKVEDFKSQAGLSFGGGIKLYKFNVGFGMSTYQVGNSVYQFSVGTSL
                     SEFGL"
     gene            8258..9409
                     /locus_tag="PLZ54_05165"
     CDS             8258..9409
                     /locus_tag="PLZ54_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOJ66354.1"
                     /translation="MAKILIIDDERSIRNTLKDILEFEKHQVSLAENGLKGLEAAQNE
                     MFDLIFSDIKMPEMDGIELLTQLKEKDIEAPIVMISGHGNIETAVECIKKGAFDFIEK
                     PIDLNRLLITVRNALEKKDLVVETKNLKKKTAKKYEMIGQSAALENVRQMIERVAPTD
                     ARVLITGENGTGKELVARLIHDKSNRANAPFVEVNCAAIPSELIESELFGHEKGAFTS
                     AIRQRIGKFEQADNGTIFLDEIGDMSLSAQTKVLRVLQENELSRVGSDKSIKVNVRVI
                     AATNKDLKKEIEKGNFREDLFHRLNVIPIHVPTLDERKEDIPLLVEHFTELICSEEGI
                     HPKKFEPDAIKALQQRSWTGNIRELRNVVERLIILGSPMITKDDVEKFA"
     gene            9533..9982
                     /gene="rpiB"
                     /locus_tag="PLZ54_05170"
     CDS             9533..9982
                     /gene="rpiB"
                     /locus_tag="PLZ54_05170"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOJ66355.1"
                     /translation="MKPLNELKIAICSDHAGFELKNKLIEYLKSKNVGQIKDFGAYTA
                     ESSDYPDFAHPMASAVENGEFDFGISLCATGNGINMTVNKHKGIRAALCWMPEIASLA
                     RRHNNANVLSLPARFINENEAKQIVDVFFETDFEGGRHQRRIDKISC"
     gene            9998..10756
                     /locus_tag="PLZ54_05175"
     CDS             9998..10756
                     /locus_tag="PLZ54_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOJ66356.1"
                     /translation="MLSKNKIKFINSLNLKKFRDETNMFIAEGTKLINDLSVTFHCEL
                     LVVTPEWLENNTPIRTKELLEVNFEEFKKISNQKNPQGILAVFGKATDIALSLEEIGS
                     ELSLALDEIQDPGNMGTIIRIADWFGIKHIFASQTSADAFAPKVVQASMGALARVQVQ
                     EVDLENFLLPLKDKLPIYGTFLKGENIYTTPLSSNGIIVLGNEGNGISPAIEKLVTER
                     LFIPNYPPGNTTSESLNVSVAAALVCAEFRRRLK"
     gene            complement(10789..13215)
                     /locus_tag="PLZ54_05180"
     CDS             complement(10789..13215)
                     /locus_tag="PLZ54_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ66357.1"
                     /translation="MKKVFFISFVFLFLSFEILASTVIKGRVIDARSGTPLEFVNVAL
                     YQLGSSVPSVGLTTDAKGNFEISSISSGKYVLKISMVGYQAYDKEVDIRSNILELGVI
                     RLEEESKVLNEVVVVAQGSQMKFDIDKKVFSVDQNIASSGGSATDVLQNIPSVEVDNE
                     GNVSLRNDANVEVWINGKPSGLTVENRAQVLQQMPAESIESIEIITNPSAKFNPEGTA
                     GIINLVLKKNRRGGYYGSLSAGVMYPDGAKLGSNGGINFNYSSSKIDGYVNMGYRSMN
                     MKGGGWNNRYNYLENDTTLLKQNNSSENNFDGVFMRAGLDWHLNDNNTFGISGFGMTH
                     NEDGNNTINYGLYDVDNFLLKNYSRYNLDNGNHPSFHLNVDYQHEFGRKDNNIMASIS
                     YSKHNRKSDDQYIQRDYLLDLTSSDITQTTDNRNHQWEFKIDYSNKITENSRLEAGWQ
                     SNLQERNNLTSAIDNLNNGVEIFSYSDNFDYKEQIHAAYLTYGNKFDQLSLQAGLRGE
                     YLEKKINNLETQSYFELFPSFYLSYSLPEQNELQLSYTRRINRPRGRMINPFRDYSDS
                     TSISYGNPQLLPEFASSLELNYLKTWNQHSLSASAYYRFTDNVIENVRYMNGQTMEST
                     YFNITKSKNAGLELVAKNNLFKILSLTSSLNLYYSQLDSASFTSPYNTTVTIPNQDDF
                     SWSGRLLANLMLSKNTSAQITGRYLSPRIISQGERAANYSLDLGFRQTFFDRKLNLNL
                     MVRDLLNSRKWKSTTWGTDFYQVSESYFHGRMVGMTLSYNFGNMKPKQTVKKRAESES
                     PLNMEMENIE"
     gene            complement(13558..>14808)
                     /gene="pyk"
                     /locus_tag="PLZ54_05185"
     CDS             complement(13558..>14808)
                     /gene="pyk"
                     /locus_tag="PLZ54_05185"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOJ66358.1"
                     /translation="KGPEVRTTIAENDKIILHAGDTIKIIGDPDKISTNECVAVSYPF
                     FVRDLHVGDDILFDDGEIELKVAGKDEQYLYCNVLNDGVLGSKKSVNVPGVRINLPSL
                     SARDKENILFAIENNLDFIAHSFVRNKQDILDIQQILDQHNSPIKIISKIENQEGVDN
                     IEEILEYTYGVMIARGDLGIEVPQEKIPGIQRLLIRKCVMAKKPVIVATQMLHSMITN
                     PRPTRAEVTDVANAIYYRTDALMLSGETAYGKYPVESVRTMSKIAEEAEKTKLTENDI
                     PIPTATVDITSFLANAAVEASSKFGIRAIITDSFTGSTARNLAAYRGKNPVYAICYRE
                     RLTRELALSYGVFPKYQEEKRTNQEYLLLALRDLLDRKLLTEQDFFCYLSGSFGEGHG
                     TTFLEINQIEKVLKALNNYQLPSF"
BASE COUNT         4516 a         2984 c         2620 g         4688 t
ORIGIN      
        1 tcaaatttat ttgatttatg ttgagcgtag ctgcttcaac taagttaatt ttttttgctt
       61 acttttttgt ttcaagacaa aaaaagtaag tgaaggtttg aggcaaagtc ccgttattaa
      121 atatctgaaa ataaagaata taaatgtttt gaaatttttt tgtcatgtac taagacaaaa
      181 aaacttttta aggctctatt aattaacttt tcccttttta attttttcta catcttccca
      241 aaatgttggg taggatttgc tgaccacttc gggatgttca atcgcaatgg gaaacagcaa
      301 ggcagcggga gcaaaagcca ttgccatacg atgatcgtca taagttttaa tagaaatttc
      361 gcctgtctct tcaaaatgac aagcgtcttt tttttctcca ctccatgaca actctccttc
      421 ggcaggttcg gtaagcaaaa agccaaattt tgccaattct gtttttaaag ctgagattcg
      481 atcggtttct ttgattttca aacttttaag cccttttaaa tggaacggaa tatttttcag
      541 acaacaggta accgcaaaag tttgagccaa atcgggttga ttgataaaat cataattaaa
      601 gaaatctatc tttttcctgt tgggcgttaa taaaactccg tccgaaaaat attgaacagc
      661 aacacctaaa ttttcaaata attccgccac tttactgtcg ccttgataac tattttgatg
      721 caaacccttc agaaaaattt ctccttctcc tgcaatgctc agtatttcaa accagtatga
      781 agctgccgac caatcagact caaccgtaaa atctaccggt ttgtaaaact gtggagcaat
      841 ttcaatcctg tttgtcaaga aattgacctc aacaccaaat tctttcatca tattcttggt
      901 catcagtata taaggaagcg aaataatttc accttccaaa attatattca atccttcgtt
      961 catgtaaggc gccgtcatca tcaaagccga aatatattgg ctgcttacac ttccatcgaa
     1021 atgtatcgtt ccacctgtca atttttttcc cttaattgat aaaggtggaa aacccgactt
     1081 ctcaagatat tctatttctg cgcccaaatc attcagggct tctaccaaca gtctcacagg
     1141 tctttgtttc attcgttcac ttccggtaag cgtccattct tcttctgtca tggccaaata
     1201 agcagtcaaa aaacgcatgg ctgttccggc agctccaaca tctatgatag attgatccag
     1261 cttcaacgcc cgcagcataa ctttcgtgtc atcgcagtcc gaaagattcc gaatttcaga
     1321 tcgtgaaccc gataaagcat ttatgattag cgcacgattg ctaatgcttt ttgaagccgg
     1381 gagcaaaata tcgagatgaa tatcggctga aggaaaatat aaatgtttgg gttccataac
     1441 aagaactatt cattttacta ttgaataaaa aaaagcaaca ttctccttat atcgaaaagg
     1501 agaatattgc tacaaaatta ctgaattgcg ccggatataa aaattttaaa cggccagttt
     1561 gtaaatttcc gcaacatctt tttcggtgag ttttacaaaa cttccaaccg tacagttacc
     1621 aagtctcatg tttttcacca atgtaggaat atcctcagct ttagctccca gctgggcaaa
     1681 gcggataggc atgccgattg aaattaaaaa ctgttcaaat ttggctattc cttcttttgc
     1741 tgttatttcg ggatgttgga aatccatttc acagccccaa acgcgcacgg caaattgagc
     1801 aaagcgcata atgtcgtggt tcattacata cttcatccat gccggaaaca taacagccaa
     1861 acccgcacca tgagctacat cgtaaagtgc cgacaactcg tgttccattt gatgagttgc
     1921 ccaatcctgc tcccgtccta ctccacaaat atcgttgtga gccaccattc ctgcccacat
     1981 aatgttggca cgggctccat aatttgttgg ttcggctatt gcaacgggag cttcttttat
     2041 aattgtcagc aaaattcctt cacaaaggcg atcggtaatt tccacttccc gggtattggt
     2101 aaaatatctt tccatcacgt gggccatcat gtcggtaatg ccgcaggccg tttgataagc
     2161 tggcaaagta aacgtaagtt ccggattgag gatggaaaat acagggcgca aggcatttcc
     2221 ttctgccgaa cgttttaata agccattttc gtgggtaata actgaactgc tggatccttc
     2281 gcttccggct gctgcaatgg taagaatcgt tcctgccggc aaagcttttt caactttttt
     2341 cccggtataa aaatcccaaa aatcaccatc atacaacgct ccggctgcaa tagcttttgc
     2401 cgagtcaatt acacttccgc cgccaacagc caggataaaa tctacacctt cgtttttaca
     2461 aatttctatt cctttgtaaa ccaaaccact acgcggattg ggcaatacac cgcctaattc
     2521 cacaaaagaa attccttctt gagtcagcga tttcttgact ctgtccagta atccgctttt
     2581 aatggctgaa tttcctccaa aatgaagcag tacctttgtt cctccaaaac gtttaacgta
     2641 tttccccgcc tcgttttctc gtccttttcc gaaaacgaaa taagttggac tataaaaatt
     2701 aaaattgttc ataatcaata agttttaaaa aatctgcttt tttaattgat aacttttttc
     2761 aatctatcta aaagttcatc acccaattcg ctttttattt ttatatggtt aatgacttcc
     2821 agcacaaaag gattgttttc aaaacttcca cgaaccgatt cgaaatcaag agatttctgc
     2881 tgttcatcac catcgacacc aaatccggtt ttagcgttca aactaaattc ttttttggca
     2941 tcatcataaa taagatgatc gagtgtaaca acagaatttt tccacagttc caccatctgt
     3001 atcaaatctt cacgggtaac ttcagcaatt ggttttctcc aataaatttc caacttctct
     3061 tttgcccaag tccattcata attgtaatac gcttcgtgca ttttttcaaa ttcgctttga
     3121 atatcttcga gcgtaatcgg ttgatcttcg attttcttca tcaactgttc aatttctgtt
     3181 ttgggcgcaa tcagtcccga taaatcaatc cattcgccac tacctacaga agtatcgggc
     3241 ttcaaaattt cccgaatatc ttcaatggta ttaaaatgtt tatctttcaa tctgctgata
     3301 agcgaattac ccaaaaactt atatattcca atgtgataca ggtcaatacc tctgagtaac
     3361 gacgaatttt ttatgctgca attttggtaa gtataaattt cagtcgtttc tcccgaaatt
     3421 ttctgtaagt ttttcaggac ttcaactcct ttcatcattt tctgaatggt gtacggactt
     3481 aaaagattaa aattaatcgg atcgagtttt ttgggatctt ttcggttatc cctttttggc
     3541 catttctggg catcgcgaat tgttcccaca cttcgcaaat tcactgccgg caccaaaaaa
     3601 ctgtcggtag cattttctat cagataagaa aaaggcaaat cggaagtatc ggaatgtttg
     3661 gtgtgtcgcc ccataaccag cgtaaaagca ccaattttag ccggccaaag caaataagaa
     3721 tcgctggtag tttttgctcc gcgttcggcc agtccctgat gaatgggacc aagtttatac
     3781 atgtgattgc tttggttgga accgctgcct gcattgagaa aagaaaacat tcctgcaatg
     3841 agtaaagtag atttgtgatg cgataccgta taaggaccgg caaaaatggc ggttgcttct
     3901 ccgtgcatgc cctgacagtt ggaaaagaaa agtgaatcga gcacagaata atgtttcccc
     3961 aaaatacaac cctgaccgat aaaacaacgg gaaatcaatg tggaatcggt aaccgaaact
     4021 ccagaactga taataaaatc gtcgcatttt acaccggctc caatggttac gggagcaaaa
     4081 gcattactgt tgatgcttcc gttgatcagg cacgaagcac cttctatatt ggcacaatcg
     4141 ccaatgcgaa cattttttat actgccggca ttcacaatcc tgacattttt accaattgtg
     4201 ccggtatcgg attttaattt acaagcgtaa tcatcaataa gcttctgaat tttttgaata
     4261 agcaccggac gatggcgata taaagtaagt acatatgcca acggcgccga aagaaaatca
     4321 aaaatcggaa tttctcttcc tcctccttca ttcattacag gcacttttac tccgttgcca
     4381 aaagttgaat ccttttctac caccagcaaa tttacatttt caatgtaaga accttcgcca
     4441 atatgatagt tggctatata attggctacc tgatcaatat aaacatcatc ttctatcgtg
     4501 caattatgca gacaacaccg acgaatgccg gcatgtcttt gcaatcctcc tgccaacaca
     4561 aaaattttat tgaagttacc gagaaaaata tttccggaaa aatgaacctc cgcaatataa
     4621 tccggtttga aaccttctga aactctcacc atttcccagt tctccgccgt gcaaccttta
     4681 gaagtgagca aatgaatttc attttcagta agaagtcgat aatttttcat aagtgtaaga
     4741 attgaattgt taatttattt tactcaacca catccgtttc attttcctcc atttcaaaat
     4801 cctaataaag aaaatcgttg taaggataac gttgaacatg aatagccttg attttctgat
     4861 acataatttt tttcagttcc tcgatatttt gtttttttgt ggctgaaatg aacacacaat
     4921 cttcatgaat tttagccatc caagtttttt ggatttcctc caaagaaata ttctcaggtt
     4981 ttttcggagt caaatcatca gggtctttgg ctttgtgatt gtaagcatca attttattga
     5041 aaaccaggat catgggcttt tcttcattgg taatttcctt cagcgtttgg ttgacaacct
     5101 gaatctgttc ttcaaaattc gggtgagaca catccactac atgaatcaac aaatcggctt
     5161 cgcgaacttc atcgagtgtc gatttgaaag attcgataag atgatgaggc aactttctga
     5221 taaaaccgac cgtatcggta agaagaaaag gcaaattatc tataatcacc tttctgacag
     5281 tcgtatcgag tgtagcgaaa agcttgtctt cggcaaaaat attcgacttg ctcagcaaat
     5341 tcattaacgt tgatttccca acgttggtgt agccaaccaa cgccacccgc accaattttc
     5401 cgcgattttt tcgctgtgtg ctcatctgac ggtcgatttc ctttaagttt tgtttcaatt
     5461 gcgcaatttt tgaaagaata attcttcgat cggtttcaat ttgggtttcg ccgggtcctc
     5521 tggtgccaat tcctccctgt tgacgttcaa ggtgagtcca catacgtgtt aggcgaggca
     5581 aaagatattg atactgagcc aattcaacct gagttttggc actggcagtt tttgcgcgct
     5641 tggcaaaaat atcaagaatc agattggtac gatccaaaat tttgattttt aattccgatt
     5701 cgagatttct gatttgtcgt gctgaaagtt cgtcatcaaa tacagccata tcaacgggat
     5761 gatccgccaa atagtttttt atttgttcca gttttcccgg accgacgaaa gtttcgggat
     5821 tgggatgatc caactgttgc aaaaaaattt tttcgggaac tgctccggcg gtttttgcca
     5881 aaaaagccaa ttcttcaata tattcttttg ctttttccct gttttgttgc ggtgttacta
     5941 aaccgaccaa aatagttttt tcctgatagt tatttgtttc caattttaat attctcttaa
     6001 acaaaacacc gtttggataa attggccaaa cggtttaata tatttttttc gatatgaaag
     6061 gtcaaccgaa atagtataat gtaaggaaaa ataaaattta ctgtaattta aaattaatgg
     6121 gaagcgtaaa tcttactcta acaggttttc ctcgttgctt acccggaatc catttgggca
     6181 tggattgaat aaccctaacc gcttccttat ccagactagg atcaacacct cttacaacct
     6241 gaatgtcaac tattgaacca tcggtattca caacaaattg gcaaataaca cgtccctgaa
     6301 ttccgttttc ctgagcaatc accgggtatt tgatattttc atttaaaaat ttgaataaag
     6361 cttgttctcc accaggaaat tgaggcattg tttcaaccac ctgaaaaata acttgttcat
     6421 cttcctcgtg aataggtgcc gccataggtg ccgccacagg cgcagctata atttcaacgg
     6481 ctttattttt ggtatcttct gtttctatgt taatggtttg agtttccacc gtattttcca
     6541 ccacattgag tacttcaatt acttcaggtg caggaggagg tggtggtgga ggagtttcat
     6601 cctgtgttgt ttgaatgata tccaactctt cttccgtttt tagatcagtt tgaacgcctt
     6661 cgtaaatggt aatttctctg gctgtccact ccagagcaat ataaataaat gacaatgcca
     6721 taaccaaacc gatcagaata aataatgatt tcttattttc taatgaggct tttggtgatt
     6781 ttttttcttc cataatgata aagtcttttt tatttttcag atggtacaaa tgtacaattt
     6841 ttttttaata gttgatgttt tatgggtaaa aaaaattttt cttttaaaca aaatcgataa
     6901 caaaattata tttttcataa aaagtcatct ccgaacgtac ctttttctta attttgccgt
     6961 tatttttcaa ttaacattgg tagttcttaa atgcattgca aaattaccaa aactttgata
     7021 tattttgcat agaacggaaa aaatctttat tctacaatat ttttaaaaaa ttttttgaag
     7081 aaactaatga taaaaagaat tgttttgttt ttatggttcc taataacttt tgttgcctta
     7141 gtttatccac aggcaggcaa aggtgtatat caatttctgg atttgcctgt atcttcacgt
     7201 attgccgcaa tgggaggaac caatgtttcg ctgaaagaca acgatataaa tttttctttt
     7261 tgcaatccgt cgctgcttac cgacaaaaca aatgattttc tttcattaag catggccaac
     7321 tatttggctg acattcagta tggaagtgcc gtgtttggaa aaaatttcaa aaacaagcat
     7381 tacatggctt ttggcatgca gtatgtcgac tacggcactt ttcaggaaac aacggaacaa
     7441 aacgaaatta ttggtgaatt tacagccaaa gacatggcac tttacataat gtatgcctac
     7501 ccgattaccg aaaaaatttc tgttggggga acttttaaac cgatttactc ggcttatgaa
     7561 cgttattcga gttttggggc agccatagat gcgggcatca gttataacga cccaaccaat
     7621 tttttttcgg caggattggt attgcgcaac ataggcagcc aaataaaagg ttattattcc
     7681 gatgaagaag gacagcatta cgaaccgctt ccatttaaca tccaattggg aataaccaaa
     7741 aaattggctt atgcgccgct gcgttttacg gggactttac acaatttgca gcactgggat
     7801 ttatcttacg cttcttccac gcagaatgaa acgaaaaaaa ttaatttttt tgacatggct
     7861 ttgcgtcatt ttattttcaa tgccgaattt attccttcca ataattttta tctgacattg
     7921 ggatataatt ttaggcgtgc aaaagaaatg aaagtagaag attttaaatc gcaggcaggt
     7981 ctttcttttg gcggaggcat taaattatac aaattcaatg ttggttttgg catgtctacc
     8041 tatcaggttg gaaacagcgt atatcaattt tctgttggca catcgctctc cgagtttgga
     8101 ctgtagtctg tttcctactt tcgacttttt cctgaaatca ttcaaagcat ccggaaattt
     8161 ttaaaatttt aaagtccgtc agcttctgca attaaataac aaagatattt tttaactttg
     8221 tttctgaaaa acaaaccccg aaagaaataa tttgaaaatg gccaaaatat taattattga
     8281 tgacgaacga agtatccgta atactttgaa agatattctg gaatttgaaa aacatcaggt
     8341 ttcacttgcc gaaaacggtt taaaaggcct tgaagcagct caaaacgaaa tgtttgattt
     8401 gattttttcc gatataaaaa tgcccgaaat ggatggtatt gaactgctta cgcaactgaa
     8461 agaaaaagac attgaagctc ctatcgtgat gatttccgga catggaaata tagaaacagc
     8521 cgtagaatgt ataaaaaaag gagcttttga ctttatcgaa aaacccatcg atttgaaccg
     8581 tctcttgatt acagtacgta atgcgctgga aaagaaagat ttggttgtgg aaaccaaaaa
     8641 tctgaaaaag aagacagcaa aaaaatatga aatgattggt caatcagccg cattggaaaa
     8701 cgttcgccaa atgattgagc gcgtagctcc cactgacgcg cgcgttttga ttaccggtga
     8761 aaatggaacg ggaaaagaat tggtagccag attaatacat gataaaagca accgggccaa
     8821 tgctccattt gttgaagtaa actgtgcggc cattccttcg gaactgattg aaagtgaact
     8881 tttcggacac gaaaaaggag ctttcacttc tgccatccga caaaggatcg gtaaatttga
     8941 acaagccgac aacggaacca tctttctcga cgaaattgga gatatgagtc tttcggctca
     9001 aacaaaagtt ttgcgggttt tgcaggaaaa tgaactttcg cgtgtgggga gcgacaaaag
     9061 tatcaaagtc aatgtcagag taattgccgc aaccaacaag gatttgaaaa aagaaatcga
     9121 aaaagggaat ttccgcgaag atttgtttca tcggcttaac gttatcccaa ttcatgttcc
     9181 aactttggac gaacgaaaag aagatattcc cttgctggta gaacatttca cggaattaat
     9241 ctgttctgag gaaggtattc atcccaaaaa atttgaaccg gacgccataa aggctctaca
     9301 acaacgctcg tggacgggaa atatacgcga actgcgcaat gtggtggaaa gattgattat
     9361 tctcggttct ccgatgatta ccaaagatga tgtggaaaaa tttgcatgaa tcgtacacaa
     9421 tccactgccg gtgcagcaac caaatttttt caaacagtca gaaaaaattt tttccggaca
     9481 aataaatcaa tgcaaggaat ttcatcaatt tctttaaaat taattcaatc acatgaaacc
     9541 actcaacgaa cttaaaattg ccatttgtag cgatcatgca ggttttgaac ttaaaaacaa
     9601 actgattgag tatctgaaat cgaaaaatgt tgggcaaata aaagattttg gcgcctatac
     9661 ggcagaaagt tccgattatc ccgatttcgc ccatcctatg gcttcggcag tagaaaacgg
     9721 agaatttgat tttggcattt ccctgtgtgc tacgggaaac ggcatcaaca tgacggtgaa
     9781 caaacacaaa ggaattcgcg ctgcgctttg ctggatgccc gaaatcgcat cgctggcacg
     9841 cagacacaac aacgccaatg tactttcgtt gccggcaaga tttatcaacg aaaatgaagc
     9901 caaacaaatc gtggatgtgt tctttgaaac cgattttgaa ggaggaagac atcagcgacg
     9961 aatagacaaa atatcctgct gaaaaaacga aaaactcatg ttatcgaaaa ataaaatcaa
    10021 gtttatcaat tctttgaacc taaaaaaatt cagagatgaa accaatatgt ttattgctga
    10081 aggaacaaaa ttgattaacg atttatcggt aacatttcat tgcgaacttt tagtggttac
    10141 acctgaatgg ctcgaaaaca atacgcccat tcggacaaaa gaactattgg aagtaaattt
    10201 tgaagagttc aagaaaattt ccaatcaaaa aaatcctcaa ggtatattgg ccgttttcgg
    10261 caaagcgacc gacatcgctt tatcgttgga agaaatcggc tcagaattat cgttggcgct
    10321 ggatgaaatt caggatcccg gcaatatggg taccattatt cgaattgccg actggttcgg
    10381 cattaaacat atttttgctt ctcaaacttc ggcagatgct tttgctccta aagttgtaca
    10441 agccagtatg ggagcgctgg cacgcgtaca agttcaggaa gtcgatctgg aaaattttct
    10501 gttgccattg aaagataaac tgcctattta cggaactttt ctgaaaggag aaaatattta
    10561 tactactccc ctatcttcaa atggcattat tgttctcgga aacgaaggga acggcatctc
    10621 tccggcaatt gaaaaattgg ttaccgaacg tctttttatt ccaaactatc cacccggaaa
    10681 taccacttcc gaatcgctca acgtaagcgt agcggcagct ttggtttgtg cggaatttcg
    10741 gcgcagactc aaataaaaag cttttggcaa ctacaatgcc tttccttctt actctatatt
    10801 ttccatttcc atattcaaag gactttccga ttccgctctt tttttaactg tttgtttggg
    10861 tttcatgttg ccgaaattgt aagatagagt catgcccacc attcttccat gaaaataaga
    10921 ttccgatacc tgataaaaat cggttcccca tgttgttgat ttccattttc tcgaatttaa
    10981 caaatcacga accattaggt tgagatttaa ctttctatca aaaaatgttt gtctaaaacc
    11041 caggtcaaga gaataattgg cagctctttc cccctgtgag atgatgcggg gagataaata
    11101 acggccggtg atttgtgcag aagtattttt actgagcatt aaatttgcca gcaatcttcc
    11161 actccatgaa aaatcatcct gatttggaat ggtaacagtc gtattgtacg gactggtaaa
    11221 tgaagccgaa tccaactgac tgtaatataa atttaaagaa gaagtcagac tcaatatctt
    11281 aaacaaattg ttctttgcaa ccaactcaag accggcattt tttgattttg tgatgttaaa
    11341 gtacgtactt tccatcgttt gtccgttcat ataacgcaca ttttcaataa cattatctgt
    11401 aaagcgataa taggccgacg ccgaaagaga atgttgattc catgttttca aataattgag
    11461 ttccaacgaa ctggcaaatt ccggcaaaag ttgtggattt ccatacgata tgctggtcga
    11521 atccgaataa tctctgaaag gattgatcat gcgtcctctg ggacggttta ttcgacgggt
    11581 atagctcaat tgcaactcgt tttgttcagg taatgaatag gaaagataaa aactgggaaa
    11641 taattcaaaa taagattgag tctccaaatt gttgattttt ttttccaaat attcaccccg
    11701 aagtccggct tgcaaactca actggtcaaa tttgttacca taggtaagat aggcagcatg
    11761 tatctgttct ttataatcga aattgtctga ataactaaaa atttccactc cattgttcaa
    11821 attgtctatg gcactggtta aattgtttcg ctcctgaaga ttactttgcc aaccggcctc
    11881 caatctgctg ttttccgtaa ttttattcga gtagtcgatt ttaaattccc actgatgatt
    11941 ccgattgtcg gtagtttgtg ttatgtcgct tgaggtaaga tctaatagat aatccctttg
    12001 aatatactgg tcatcagatt ttctattatg ttttgaataa gaaatactgg ccataatatt
    12061 attatctttt cttccaaatt cgtgttgata atcaacattc agatgaaagc ttggatgatt
    12121 tccattatcc aaattataac ggctatagtt tttcagaaga aaattatcaa catcatataa
    12181 accataatta atggtgttgt ttccatcttc attgtgtgtc attccaaagc ccgagatacc
    12241 aaaagtgtta ttatcgttca aatgccagtc gagacctgca cgcataaaga caccatcaaa
    12301 attattttct gatgaattat tttgtttcag caaagtagta tcgttttcga gataattgta
    12361 tcgattgttc caacccccgc ctttcatgtt catcgatcga taacccatgt tgacataacc
    12421 atctattttg ctgctactat aattaaaatt tattccgcca ttactaccta attttgctcc
    12481 atccggatac attactccgg ccgaaaggct accataataa ccaccacgtc tgtttttttt
    12541 caaaacaagg ttgataatgc cggctgttcc ttcaggattg aattttgcag aaggattagt
    12601 aataatttca atagattcaa tgctttcggc aggcatttgt tgtaaaactt gagcacgatt
    12661 ctcaactgta agtccagaag gtttaccgtt gatccaaact tcaacatttg catcgtttct
    12721 taaagacaca tttccttcat tatcaacttc taccgaaggg atattctgaa gaacatccgt
    12781 tgctgagcct cctgaagaag cgatgttctg atcaacacta aaaacttttt tatcgatgtc
    12841 gaatttcatt tgagaacctt gagctacaac aaccacttcg ttcaatactt tcgattcttc
    12901 ttccaaacga atgactccaa gttctaaaat attgcttctt atatctactt ctttgtcata
    12961 agcctgatag cctaccatac tgattttgag aacatattta ccactgctaa tagaggaaat
    13021 ttcgaaattc ccttttgcat cggtggttaa tccgacagag ggaacagaag aacctaattg
    13081 atataaagca acatttacaa actctaatgg agtgccagac ctggcatcaa ttactcttcc
    13141 ctttataacc gttgaagcta agatttcaaa acttaagaat aaaaaaacaa aggaaataaa
    13201 aaatactttt ttcatcagaa tataatttat tttttaaagg tctgatggaa cccacatgtc
    13261 tgacatatta tattcattcg tgtttgtgta tgcaatacat gtgggtttca tacaaatatc
    13321 ttttcgtttt ataagtgttt atgaaaactt acatatttaa tggacttcaa aacaccaaca
    13381 aagtttaatt ttcgataggt ttttaaatgt tatgtaattg cataaacttc tataaaaata
    13441 ctatccaatc atttattaac gaaatgataa tcacatttac ttattcagtt aaacatttta
    13501 gcgttttaac tgtttgatgt ttttcggata ttagaaaaaa ggaattttta aataaggcta
    13561 aaaactcggt aactggtaat tattcaatgc tttaagaact ttttcaattt gattgatttc
    13621 cagaaaagtg gttccgtgac cttctccaaa acttccgctc aaataacaga agaaatcctg
    13681 ttcggtcaaa agttttctat ccagtaaatc ccttaaagcc aacaataaat attcctgatt
    13741 ggttcgtttt tcttcctgat atttgggaaa aacaccataa gacaaagcca attcgcgcgt
    13801 caatctttca cgataacaaa tggcataaac aggatttttc cctcgatagg ccgccaaatt
    13861 tctggccgtc gatccggtaa acgagtcggt aataatagcc cgaataccaa atttactgct
    13921 ggcttccaca gcagcatttg ccagaaaaga agtaatatcg actgttgccg taggaatcgg
    13981 aatatcgttt tccgtcagtt tcgttttttc ggcttcttcg gcaattttcg acatggttcg
    14041 taccgattca accggatact tgccataagc agtttctccg ctcaacatca aggcatcggt
    14101 gcgataataa atggcgttgg ccacatccgt aacttctgcc cgcgtgggac gtggattggt
    14161 aatcatggaa tgaagcatct gagtagccac aataaccggt ttttttgcca taacacattt
    14221 tctgatcaga aggcgctgaa tacccggaat tttttcctga ggcacttcta ttcccaaatc
    14281 gccgcgagct atcattaccc cataggtgta ttcaagaatt tcttctatat tgtccacgcc
    14341 ttcctgattt tcgattttcg aaataatttt gatcggactg ttgtgttgat ccagaatttg
    14401 ctgaatatcg agaatatcct gcttgtttcg aacaaatgaa tgagctataa aatcaagatt
    14461 gttttcaatg gcaaataaaa tattttcctt gtcgcgtgct gacaaggatg gcagattgat
    14521 gcgtacaccg ggaacattca cacttttttt acttcccaat acaccatcat tcaacacatt
    14581 acaataaaga tactgctcat ctttccctgc cactttcaac tcgatttctc catcgtcgaa
    14641 aagaatatca tcgcctacat gtaaatcgcg cacaaaaaac ggataactga ccgcaacaca
    14701 ttcattggtc gagattttgt ctggatcgcc aataatttta atggtgtcgc cggcatgtaa
    14761 aattatttta tcgttttcgg caatggtcgt tcgcacttca ggaccttt
//
LOCUS       DAOSCH010000049        14570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2804_length_14570_cov_6.239614, whole genome shotgun sequence.
ACCESSION   DAOSCH010000049 DAOSCH010000000
VERSION     DAOSCH010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14570
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2804_length_14570_cov_6.239614"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..73)
                     /locus_tag="PLZ54_05190"
     tRNA            complement(2..73)
                     /locus_tag="PLZ54_05190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(38..40),aa:Arg,seq:cct)
     gene            171..803
                     /locus_tag="PLZ54_05195"
     CDS             171..803
                     /locus_tag="PLZ54_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOJ66359.1"
                     /translation="MSKNKFFVVWKGKQPGIYSSWEECKRQVHQFEGALFKGFATEEE
                     AKKAFESLPWKYIDTRNNAQNKSSREKMPIGQPIMESISVDAACSGNPGLLEYRGVYT
                     KTGKEIFHQGPFRNGTNNIGEFLAIVHALALMKQNNSQLPLYSDSRTALKWVQQKKAK
                     TKLEKNEENKSLFELIERAENWLQNNDYSTPLLKWETEAWGEIPADFGRK"
     gene            complement(805..2196)
                     /locus_tag="PLZ54_05200"
     CDS             complement(805..2196)
                     /locus_tag="PLZ54_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66360.1"
                     /translation="MNKKLSLGTRFVLLSIIVFFVFIALTGLIYRYTAIGIEQNIPVL
                     TLLAGSYFFVQLILFLVIFKSHLPKWNFSWDNILTVSLFFLLISTVLSVNVSITATVL
                     FVVLAVLFAIVKKKHFQPHPLFYFMGAYYLFQYVGLLWSIDPAYGWRFVGRGLSFIVL
                     PLAFGFYSVSEEKKNLLLRIFFRFLEVYLLMVIVAYFYQVEFHKVDFGVGFHLKKNYF
                     CTPVPPGVDYYLLLGWAGYNHPTFVSFILTLMFGIGFYLWRNDNSPQTVKISGFEMGF
                     YGFMTAVVILLLQSRIGMIMFPFGIFLTLLYSLRKKKKLVLSVIVFSAFLVLSAGFYI
                     YKNYRGYFYDRPREIQTDIVLSFLKDHYLLGTGTGGMKVLIPWFPTAHNQFLGDLFHL
                     GIPGLLLLLSLMGSSFYFACKDKNFLLLYFLLIYFVMMQIEMPLSLQKGITYFTLFTG
                     LLLKPRPFGKETK"
     gene            complement(2205..3698)
                     /locus_tag="PLZ54_05205"
     CDS             complement(2205..3698)
                     /locus_tag="PLZ54_05205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ66361.1"
                     /translation="MENPLVSVIIPVYNMEAFLAEAIDSVLASTYPNFEIIVVDDGST
                     DQSPVIAEHYSERDTRIRFYRQSNRGVAAARNLALQEARGEFVLPVDADDKISEIFLE
                     TAAKVLVQLPQVKIVTCEYEYFGEKQGKGKYRPYHRKLIARKNVINNCSMYRRRDALK
                     VGGYCEEIIGREDWEFWISLLKNGGEVEHLSETGFYYRIRSNSKRITDRKLKKHIIDV
                     LNRRHKAFFYRELGGKLHYRRSLSRLLNFFYHCYKPETFFVHPDFKEEEEFFYSLPDI
                     FDNQGETIHSGRNIIKIFNAGNRKWVVKSFQSPNVVNRFIYGIFRKSKARRSYEYALK
                     LLSLGIATPQPIGYYEQKIFFLFNKSFYVSESSECNHRFEELIRDVNFPDREKILKAV
                     ARFTAELHEKGVLHSDYSAGNILFSQKNDDIHIELIDLNRLKFGKISKTAGCRNFERL
                     NLEPEALKTMAEEYAVARGFEKQECVELVQRYRWKKHKNISSLQPKN"
     gene            complement(3703..4425)
                     /locus_tag="PLZ54_05210"
     CDS             complement(3703..4425)
                     /locus_tag="PLZ54_05210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ66362.1"
                     /translation="MNQQALHIITPVKDSLETTLQTIGSIMDSDFHSDFQYTVYNDFS
                     SDETTEKLEIVSAEKGFRLVNLKDITTHPSPNYLLVLQLAQQKALSEHAHLLVVESDV
                     IVKKNTIQILFDAAQKLENPGLIAAVTHDEEGNINFPYLYAKNFDSGTVQTSKRLSFC
                     CTLLSNSFLEAFDFKNLNPEKSWYDVFISHKAVELGFNNYLLTYLPVLHLPHSSRPWK
                     QLKYTHPLKYYWLKYTKGRDKI"
     gene            complement(4422..5921)
                     /locus_tag="PLZ54_05215"
     CDS             complement(4422..5921)
                     /locus_tag="PLZ54_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOJ66363.1"
                     /translation="MPEKQMKRLAKDTAIYGLSSIIGKFLNWCLVPLYTYVLESSADY
                     GIVTNLYAWTALLLVILTYGMETGFFRFANKNRDDAPKVYGTTMISVGFTSLLFATIC
                     VIFSQPIANGMGYPKYPEFIAMLAIVVSMDAFDAIPFAYLRYKNRPIKFAALKLFMIF
                     TNIFFNIFFLVICPWLQKKAPGLISWFYNPHYGVGYIFVANLISTAAVTLALLPDMLE
                     AKFRLDMALLKKMLQYSLPLLLLGIAGIIDQTIDKIIFPFLFEDKAYAAAQLGIYGAC
                     FKISMVMMMFTQAFRYAYEPFIFAQYKDKNSTAAYADAMKYFIIFSLMIFLGMVFYLD
                     IFKFIIRSDYWAGLRVVPIVLFSYLFQGVFFNLSLWYKLTDKTMYGAWISIVGSLITI
                     AINIIFVPVFSYMASAWASFACYFAMMLMSYFLGQKYMPIKYDLKTIGLYTIVTLALY
                     CISLLIATPYLWLNLLLKTILFVLFVALLIKRDFPLQSIPVINRYLKKS"
     gene            complement(5974..6987)
                     /gene="ruvB"
                     /locus_tag="PLZ54_05220"
     CDS             complement(5974..6987)
                     /gene="ruvB"
                     /locus_tag="PLZ54_05220"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOJ66364.1"
                     /translation="MTENFDIRSNNIEKDFENALRPLTFSDFEGQNKIVENLKVFVQA
                     ARMRSESLDHVLLHGPPGLGKTTLSNIIAHELNVGIKITSGPVLDKPGDLAGLLTSLE
                     PNDILFIDEIHRLSPVVEEYLYSAMEDYRIDIMIDKGPSARSIQLELNPFTLIGATTR
                     SGLLTSPLRARFGINCHFEYYDTEVITHIIKRSAYLLNVQIEERAAIEIGSRSRGTPR
                     VANALLRRVRDFAQVKGSGKIDLEIACYALEALNIDKYGLDEIDNKILTTIIDKFNGG
                     PVGLSTIATALGEDAGTLEEVYEPFLIMEGFLKRTPRGREVTELAYRHLGKSKMGMMD
                     TLF"
     gene            complement(7051..7962)
                     /locus_tag="PLZ54_05225"
     CDS             complement(7051..7962)
                     /locus_tag="PLZ54_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ66365.1"
                     /translation="MSINITKLSKNFGQQKVLDDVCFDIDKNGVVGFLGPNGAGKTTT
                     MRIIAGALPYETGSVKVCGHEVKDAPLKIKSLIGYLPEQNPLYEEMYVKEYLLFIADV
                     YKLGQYRNKKVDEMIDKVGLRAEFRKKIGQLSKGYRQRVGLAQALIHEPKVLILDEPT
                     TGLDPNQLEEIRTLILELGKDRTVLLSTHIMQEVKALCNRVIIINKGKIAADFEELSS
                     IHSLNRDIFQVEVEFFKKPAAKDIENIPGVLKVNFLDETRLLIVASEDIRSEIFDFSV
                     KQGNKILLMKLVEQEIEQVFRSLTAES"
     gene            complement(8110..8778)
                     /gene="pssA"
                     /locus_tag="PLZ54_05230"
     CDS             complement(8110..8778)
                     /gene="pssA"
                     /locus_tag="PLZ54_05230"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOJ66366.1"
                     /translation="MKKHIPNFLTCLNLFSGCIGVYYAFQGNYFAVLVFILIAAFFDF
                     IDGLAARLLQAYSPMGKELDSLADVVSFGLAPGAVCFSLLSQSSLPFWVAFAGFVITL
                     FSALRLAKFNIDERQGSSFLGLATPANAIFWVGLGYSFSEFVTANSWLILILIVIFSA
                     LLVSEIPMFSLKFKNLKWEENELQYIFLMLCVLFLFVFQLNAFVPIIIAYLFLAVVKV
                     VFKI"
     gene            complement(8910..9356)
                     /gene="dtd"
                     /locus_tag="PLZ54_05235"
     CDS             complement(8910..9356)
                     /gene="dtd"
                     /locus_tag="PLZ54_05235"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOJ66367.1"
                     /translation="MRVLIQRVAEASVEIDRQIISSIGKGLLVFVGIESTDTLQDAEW
                     LAGKTAALRIFDDEKGVMNLSVTDIDGEVLVVSQFTLCAETRKGNRPSYIRAARHETA
                     IPLYESFCKIMEQKTGKEVKTGVFAADMKVKLVNDGPVTIWIDSKV"
     gene            complement(9404..10504)
                     /gene="ychF"
                     /locus_tag="PLZ54_05240"
     CDS             complement(9404..10504)
                     /gene="ychF"
                     /locus_tag="PLZ54_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOJ66368.1"
                     /translation="MALQCGIVGLPNVGKSTLFNCLSNAKAQAANFPFCTIEPNVGVT
                     QVPDERINILARLANPQRLVPATVEIVDIAGLVKGASKGEGLGNKFLANIRETDAILH
                     VLRCFEDENVAHVNGVIDPVRDKEIVDAELQLKDLETVESRINKVQKQAQTGGDKQAK
                     IIYEILQEYRSALLQGKSARTVVLDKESKKLVKDLFLLTDKPVMYICNVDEASATSGN
                     AYVEAVREAVKEENAEILVVAAATEAEIAELESYEERQMFLEELGLKESGVSRLIQSA
                     YRLLNLETFFTVGPLEVRAWTYEKGWKAPQCAGVIHTDFEKGFIRAEVIKYEDFVALG
                     SETACREAGKIYIEGRDYVVQDGDIMHFRFNV"
     gene            complement(10726..11292)
                     /gene="purN"
                     /locus_tag="PLZ54_05245"
     CDS             complement(10726..11292)
                     /gene="purN"
                     /locus_tag="PLZ54_05245"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008641775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOJ66369.1"
                     /translation="MSKNIALFASGSGSNAENIIRYFTGNSDFSFPLIISNNEKAFVH
                     ERAKKLGVPSLTFPREDFIDGGKILNLLQEKNIDAIVLSGFLLKIPDSIIEAYPQKIV
                     NIHPALLPKFGGKGMYGMNVHRAVLEAGEKETGITIHYVNSNYDEGDIIFQAKCPVLP
                     DDTPESVAQRVHALEYEFFPRIIAEIWK"
     gene            11444..11680
                     /locus_tag="PLZ54_05250"
     CDS             11444..11680
                     /locus_tag="PLZ54_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOJ66370.1"
                     /translation="MSEVEEKVKTIIADKLGVDEAEITPTASFTNDLGADSLDTVELI
                     MEFEKEFGISIPDDQAEKITTVGDAVSYIENAKK"
     gene            11696..12958
                     /gene="fabF"
                     /locus_tag="PLZ54_05255"
     CDS             11696..12958
                     /gene="fabF"
                     /locus_tag="PLZ54_05255"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOJ66371.1"
                     /translation="MELKRVVVTGLGAITPLGSSVPKLWENILNGVSGAGPITHFDAS
                     QFKTQFACEVKDFDPFQYFEKKEIRKVDPYAQYAIAAAKQAIENCGINLETVDKDEVG
                     VIFSSGIGGITTFEQEVSNYFLNKEMGPKFNPFFIPKMISDIAAGQISILYGFKGPNY
                     ATTSACASSTHSIIDAFNYIRLGKAKMFVVGGAEAAITAAGVGGFNSMMALSTRNDDP
                     AGASRPFSKSRDGFVIGEGAGCMILEELDHALARGANILCEVAGGGMSADAYHLTASH
                     PEGLGAILAMKSALKDAGMQVEDIDYINVHGTSTPVGDTSEAKAIENVFGEQAYQLNI
                     SSTKSMTGHLLGAAGAVEAIICTLAIINDVVPPTINFTEGDEDPDINYRLNFTFNKPQ
                     KRTINAALSNNFGFGGHNASVILKKFTK"
     gene            12963..13730
                     /gene="rnc"
                     /locus_tag="PLZ54_05260"
     CDS             12963..13730
                     /gene="rnc"
                     /locus_tag="PLZ54_05260"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOJ66372.1"
                     /translation="MKNFFHLNGLFSKYRKEPYSLFRKLLGFYPRDINYYLLALTHRS
                     LSMTNHDGKVVSNERLEFLGDAVLNAIVSDLFYRYYEQKDEGFLTNIRSKIVNRDSLN
                     HLALKLELDKIVKVSKNVNPQVSNDIYGNALEALIGAIYLDYGYNHCMKFVKTKLFEP
                     FIDLSKVEKFEMNYKSKLIEWCQKNHFQIDFEMSCKTHNENHQPIFLSRVLIEGRPVS
                     ESTGLNKKESQQKASRIAYQQIIEQKTFHFINEESKN"
     gene            13884..14084
                     /locus_tag="PLZ54_05265"
     CDS             13884..14084
                     /locus_tag="PLZ54_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOJ66373.1"
                     /translation="MSTENIGINAGAVWKVLNEKGEMDVKALKKSAKLSEKDLNMALG
                     WLAREGKIEFNETEENLLVHLV"
     gene            14234..14306
                     /locus_tag="PLZ54_05270"
     tRNA            14234..14306
                     /locus_tag="PLZ54_05270"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14267..14269,aa:Gly,seq:gcc)
     gene            14332..14418
                     /locus_tag="PLZ54_05275"
     tRNA            14332..14418
                     /locus_tag="PLZ54_05275"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14365..14367,aa:Leu,seq:taa)
BASE COUNT         4841 a         3081 c         2555 g         4093 t
ORIGIN      
        1 gtagtctcgc cgggaatcga acccgaatct aaagtttagg aaactttcgt tctatccatt
       61 gaactacaag accatttttt tgaagtggtg caaaattaca ttttttcggc gataaataaa
      121 aatttacttc ccccttctct atccttcaca tcctgaaaac tatcaaccgt atgagcaaaa
      181 acaaattttt tgttgtctgg aaaggaaaac agcccggtat ttattcttca tgggaagaat
      241 gcaaacggca agttcaccaa tttgaaggcg ccctattcaa aggttttgct actgaagaag
      301 aagcaaaaaa agcattcgaa tcccttcctt ggaaatatat cgacacacga aataatgcgc
      361 aaaacaaatc ttcaagggaa aaaatgccaa tcgggcaacc gataatggaa agtatttcgg
      421 tagatgcagc ctgcagcggc aatccgggac tgctcgaata tcgcggagtt tataccaaaa
      481 caggcaagga aatttttcat cagggaccat ttcggaacgg aacaaacaat attggcgaat
      541 ttttggctat tgtccacgca ctggctctaa tgaaacaaaa caacagtcag ttgccactct
      601 actccgacag ccgcaccgca ctgaaatggg tacagcaaaa aaaggcaaag accaaacttg
      661 aaaagaatga agaaaacaaa tctttgtttg aactgataga acgtgcagaa aactggctgc
      721 aaaacaatga ttattctact ccgctgctga aatgggaaac tgaagcttgg ggcgaaattc
      781 cggccgattt cgggagaaaa taacttactt tgtctccttt ccaaaaggtc gaggtttgag
      841 caataatccg gtgaataaag taaaataggt tattcctttt tgcaaggaaa gcggcatttc
      901 tatttgcatc attacaaaat aaatcaacag aaaatagagc agcaaaaaat ttttatcctt
      961 gcaggcaaaa taaaacgaac ttcccattaa agacagtaac aagagcaagc cgggaatgcc
     1021 caaatgaaac aagtctccta aaaattgatt gtgagctgtt gggaaccaag gaattaggac
     1081 tttcattcca ccggtaccgg tacccaacaa ataatgatct tttaaaaacg aaagtacaat
     1141 gtcggtttga atttcgcgag gacggtcgta aaaataaccg cgataatttt tataaatata
     1201 aaatccggct gacaaaacta aaaaagctga aaaaacaatt actgaaagaa caagcttttt
     1261 cttttttcgc agactgtaaa gcaaagttaa aaaaatgcca aacggaaaca taatcattcc
     1321 aatacgactt tgcaacaata aaatgactac ggctgtcatg aacccataaa aacccatttc
     1381 gaaaccggat attttaactg tttgcggtga attgtcgttt cgccacaaat aaaacccgat
     1441 tccaaacatc agcgtcaaaa tgaatgaaac gaaagtcgga tgattgtagc cggcccaacc
     1501 caacaacaaa tagtaatcca ctccgggagg tacaggtgtg cagaaataat tctttttcag
     1561 atgaaatcca actccaaaat caactttatg aaattcaacc tgataaaaat aagccactat
     1621 taccattaat aaataaactt ccaaaaaccg aaagaaaata cgcagcaaca aatttttctt
     1681 ttcctcggag acagaataaa aaccaaaagc caaaggtaaa acaataaaag acagccctct
     1741 tcccacaaaa cgccaaccat aagcaggatc gatcgaccac agaagcccaa catattgaaa
     1801 caaataatag gctcccataa aataaaaaag cggatgaggt tgaaagtgct tcttttttac
     1861 tatcgcaaac agcactgcca aaacaacaaa aaggacggtg gccgtaatcg aaacattaac
     1921 acttaaaacc gtagaaatca ggagaaaaaa caacgaaacg gtcagaatgt tgtcccatga
     1981 aaagttccat tttggtaaat gacttttaaa aatgacaaga aaaagtatca gttgaacaaa
     2041 aaaataactc ccggccaata atgttaaaac aggaatattt tgctcaatgc caatagccgt
     2101 atatcgataa attaagccgg tgagggctat aaatacaaaa aaaacaatta tcgacaaaag
     2161 aacaaaacga gttcccaaag aaagtttttt attcattcca atgtctaatt tttaggttgt
     2221 aacgagctga tatttttatg ttttttccaa cgataccgtt gaacgagttc tacacattcc
     2281 tgcttttcaa aaccacgagc cacagcgtat tcttcagcca ttgttttcaa agcttcgggc
     2341 tccagattca atctttcaaa gttcctgcag ccggctgttt tggatatttt tccaaatttc
     2401 aaccgattca aatcaatcaa ttcaatgtga atatcgtcat tcttttgaga aaacaaaatg
     2461 ttccctgccg aataatccga atgtaaaact cctttttcgt gtagctcagc cgtaaaacgt
     2521 gcaacagctt tcaatatctt ttctcggtcg ggaaaattca cgtctcgaat caattcttca
     2581 aaacgatgat tgcattcaga gctctccgac acataaaaac ttttattgaa tagaaaaaaa
     2641 atcttttgct cataatagcc aatcggttgt ggagtggcaa ttccaagcga gagaagtttc
     2701 aatgcatatt catacgaacg tctcgctttc gattttctga aaataccata tataaagcga
     2761 ttcaccacat ttggcgattg aaacgatttc acaacccatt tcctgtttcc cgcattaaaa
     2821 attttaatga tatttcttcc cgaatgaatc gtttctcctt gattgtcaaa aatatccggc
     2881 aacgaataaa aaaattcttc ttcctctttg aaatcgggat gaacgaaaaa agtttcgggc
     2941 ttgtaacaat gataaaaaaa attcaacaag cgagatagtg agcgtctata atgaagtttt
     3001 ccacctaatt cgcgataaaa aaaagcttta tgtcttcggt ttaaaacgtc gataatgtgt
     3061 tttttcagtt ttctgtcggt aattctttta gaattactcc tgattcgata gtaaaatcct
     3121 gtttcagaaa gatgctccac ttctcctcca tttttcagca acgatatcca aaattcccag
     3181 tcttcacgac caataatttc ctcacaataa ccgcccactt tcaaagcatc tctcctgcga
     3241 tacatcgaac aattattaat aacattcttc cgagcaatca attttctgtg ataaggtcga
     3301 tatttccctt taccctgttt ctcgccaaaa tattcatatt cacacgtaac aatctttact
     3361 tgaggcaatt gaacaagtac tttagcagca gtttcaagaa aaatttcaga aatcttatca
     3421 tcggcatcta ccggcaaaac aaattctcca cgagcttcct gcaaggcaag attacgggct
     3481 gctgccactc cacgattgga ttgacggtag aatcggattc gtgtatccct ttcactgtaa
     3541 tgttctgcta tcactggcga ctggtctgta gaaccatcgt caacaacaat tatttcaaaa
     3601 tttggataag ttgaagccag tacagaatct atcgcttctg ccagaaacgc ttccatattg
     3661 tacaccggaa taatgacaga gaccaaagga ttttccattt tctcaaattt tatctctacc
     3721 ttttgtatat ttcagccagt aatattttaa aggatgcgtg tatttaagtt gcttccacgg
     3781 acgactgctg tgaggtaaat gcaaaacagg caaataggta agtaaataat tgttgaatcc
     3841 caattctaca gctttgtgcg aaataaacac atcataccat gacttttcgg gattcaaatt
     3901 tttgaaatcg aatgcttcta aaaacgaatt ggagagcaat gtacagcaaa aactcaaacg
     3961 tttggaagtc tgcaccgttc cactgtcaaa atttttggca taaagatacg gaaaattgat
     4021 atttccctcc tcatcgtgcg ttacagcagc aatcagaccc ggattttcaa gtttctgagc
     4081 agcatcaaac aaaatttgaa tggtgttttt tttcactatt acatccgact ccacaaccag
     4141 caaatgtgca tgctccgaca aagctttctg ttgggccagt tgcaatacca gcaaatagtt
     4201 tggagaggga tgcgtggtaa tatctttcaa atttaccaac ctgaatccct tttcggcgga
     4261 aacaatctcc agtttttcgg ttgtttcgtc tgaactgaaa tcattataaa cggtatattg
     4321 aaaatctgaa tgaaaatcgg aatccatgat acttcctatg gtttgcaggg ttgtttccaa
     4381 cgaatccttc acaggcgtaa taatatgcaa ggcttgctga ttcatgattt tttcagataa
     4441 cgattgatta caggaattga ctgtaacggg aaatcacgtt ttattaataa agctacgaaa
     4501 agaacaaaca aaatggtttt caacagcaaa ttaagccaca aatagggcgt tgcaatcaaa
     4561 agactgatac agtacaaggc aagagttaca atggtgtaga gaccgatagt ttttaaatca
     4621 tatttaatcg gcatatattt ctgtcccaaa aagtaagaca tgagcatcat cgcaaaataa
     4681 caggcaaacg aagcccacgc cgaagccata taactgaaaa cgggaacaaa aatgatattg
     4741 atggcaattg taatcaaact tcccacaatc gaaatccaag ctccatacat ggttttgtcg
     4801 gtaagtttgt accaaagcga aagattaaaa aacacaccct gaaacaaata ggaaaacaat
     4861 acgatgggaa caacacgcaa ccctgcccag taatcgcttc ggataatgaa tttgaaaata
     4921 tccagataaa aaaccatacc caaaaaaatc atcaaggaaa aaatgatgaa atatttcatc
     4981 gcgtcggcat aggcagctgt actgttctta tccttatatt gcgcgaaaat gaaaggttca
     5041 taagcataac gaaaagcctg agtaaacatc atcatcacca tcgaaatttt gaaacaggcg
     5101 ccgtaaattc ccaattgagc agctgcatag gctttgtctt cgaaaagaaa gggaaaaata
     5161 attttgtcga tggtttgatc gataattcct gctattccca ataagagtaa gggcaatgaa
     5221 tattgaagca ttttcttcaa caaggccatg tccaaacgga atttagcttc caacatatcg
     5281 ggcagcaaag ccagtgttac agcagcagtc gaaatcagat tggctacaaa aatataaccc
     5341 actccgtaat gtggattgta aaaccaactg attaaaccgg gcgctttctt ttgcaaccac
     5401 ggacaaataa ccagaaaaaa gatattgaaa aaaatgttgg taaaaatcat gaaaagcttt
     5461 aatgcagcaa attttatggg acgattttta tagcgcaaat aagcaaacgg aatggcatcg
     5521 aaagcatcca tagaaaccac tatggccagc atagctataa attcgggata tttaggataa
     5581 cccattccat tggcaatggg ttgcgaaaaa atcacacaaa tggttgcaaa aagtaaggaa
     5641 gtgaacccca ccgaaatcat ggtggtacca taaactttgg gtgcatcatc ccgattttta
     5701 ttggcaaaac ggaaaaatcc cgtttccatt ccgtaggtga gtatcaccag caacagagcc
     5761 gtccaagcat acaggttggt aactattcca taatcagccg aactttccaa cacgtaggtg
     5821 tacaacggca ccaaacacca gttgagaaat ttccctatga tgctgctcaa tccgtaaatg
     5881 gctgtatctt ttgccagcct tttcatctgc ttttccggca tattctcttt taaatatatt
     5941 ttacaatggc tcggttgaaa ctgctaaaat ttttcaaaaa agtgtatcca tcattcccat
     6001 tttcgacttc cccaagtgtc tgtatgccaa ttcggtaact tctcttcctc gtggcgtgcg
     6061 tttcagaaag ccttccatga tcagaaatgg ttcatatact tcttccaacg tgccggcatc
     6121 ctcgccaagt gctgtagcaa tagtagacaa cccaacaggg ccaccattaa atttgtcgat
     6181 aatggtagtt aaaattttat tgtctatttc gtccaatcca tatttgtcga tattcagagc
     6241 ttcgagcgca taacaggcaa tttccaaatc gattttgccg cttcccttaa cctgtgcaaa
     6301 atcgcgtaca cgccgcagca aagcatttgc cacgcgaggt gttccccgac ttcgactgcc
     6361 aatttctatg gctgccctct cttcaatttg cacattcaac aaataagcag aacgtttaat
     6421 aatatgcgta atcacttctg tatcgtaata ctcgaaatga caattgatac caaatctggc
     6481 tcttaaggga gaagtcaata atccgctgcg agtagttgct ccaatcagtg taaaaggatt
     6541 cagctccaac tgaatggaac gcgcacttgg acctttgtcg atcataatat ctatccggta
     6601 atcttccata gccgaatata aatactcttc cactaccgga ctcagtctgt gaatttcgtc
     6661 aataaacaaa atatcgttcg gttccaggct ggtgagcaaa ccggccaaat cgccaggttt
     6721 atcgagtaca ggccccgaag taattttaat gccaacattc agttcgtgag caatgatatt
     6781 ggacaaagtg gttttcccca atcccggagg cccatgcaaa agcacgtgat caagcgattc
     6841 ggaacgcatg cgagccgctt gaacaaaaac tttcagattt tctacaattt tattttgtcc
     6901 ttcaaaatct gaaaacgtca gcggacgcaa tgcattttca aaatcctttt ctatattgtt
     6961 gctccttatg tcaaaattct ctgtcatgta aaactccaac aaaaaaaatt gtatcctgaa
     7021 ttatttccgt gccaaaattt ttttccaaca ttaactttct gcggtcaaac tgcgaaagac
     7081 ctgctcaatt tcctgttcca ccaatttcat cagcagaatt ttatttccct gtttcacgga
     7141 aaaatcaaaa atctcactgc gaatatcttc cgaagccaca atcaacagac gggtttcatc
     7201 caaaaaattc actttcaata ccccaggaat attttcaata tcttttgctg ccggtttttt
     7261 aaaaaattct acttcaacct gaaaaatatc ccgatttaaa gagtgaatgg acgaaagttc
     7321 ctcaaaatct gcagcaattt ttcctttatt tataatgatt actctgttgc acaaagcttt
     7381 aacttcctgc ataatgtgag tactgagtaa cacagttcgg tcttttccca actccagaat
     7441 taaagttcta atttcttcca actgattggg atccaagccg gttgtaggtt catccaaaat
     7501 caaaactttc ggctcatgaa tcaaagcctg tgccaaccca acccgctgac gatatccttt
     7561 ggaaagctgt ccgatttttt tacggaattc agcacgaaga cctaccttat caatcatctc
     7621 atccactttt ttgttcctgt attgtcccaa tttgtaaaca tccgcaataa aaagcagata
     7681 ttcttttaca tacatttcct cgtacaatgg attttgttcg ggcaaataac caatcagcga
     7741 tttgattttc aaaggcgcgt ccttcacttc atgaccacaa acttttacac tgcccgtttc
     7801 gtaaggcaaa gctcctgcaa taattctcat cgtggttgtt tttccggcgc catttggccc
     7861 caaaaaacca actactccat ttttgtcgat gtcaaaacag acatcgtcca aaaccttttg
     7921 ttgtccgaag ttttttgaaa gttttgttat attgatggac acacgtttcg tgttttgtaa
     7981 aaagttatat tcaaaaattt cgactgcaaa agtaataaat ccttaccgta taacgaaatg
     8041 aaagagaaag cgttttaaac aagaaaaaag tcaacttcct attcggacag aacgaaacgg
     8101 cagaaaaaat caaattttga aaacaacttt aacaactgcc aaaaaaagat aggcaataat
     8161 aatgggaaca aaagcattca gttgaaaaac aaaaagaaac aaaacacaca acatcaagaa
     8221 aatatattgc aattcatttt cttcccattt caggtttttg aatttcaaag aaaacatggg
     8281 gatttccgat accagcagag cactgaaaat tacaatcaaa atcaaaatca gccaactgtt
     8341 tgccgttacg aattcggaaa aagaatagcc caatccaacc caaaaaatgg catttgctgg
     8401 agttgccaaa cccaggaatg aggaaccttg ccgctcatcg atgttgaatt tggccagtcg
     8461 cagagccgaa aacaaagtaa tgacaaatcc ggcaaacgct acccaaaacg gcaaggagct
     8521 ttgagagagc aaactaaaac ataccgctcc gggtgccaaa ccaaaactca caacatcggc
     8581 caaagaatcg agctcttttc ccataggcga ataagcctgc aacaggcgag cagccaatcc
     8641 atcaataaaa tcgaaaaaag cagcaatcaa aatgaaaacc aaaacagcaa aataatttcc
     8701 ctgaaaagca taataaactc caatacagcc agaaaataaa ttcaagcagg tcagaaaatt
     8761 cggaatatgt tttttcatac tttgtcctaa tttatggtaa atactttttt aaaaccactg
     8821 aaatcaaaaa aaatcgaaaa ctttggaaca acaaaaaatg ggacaaacat ttcttttttg
     8881 attttttctg caatgaaaca gcaaaattat caaactttac tgtctatcca aatagttacc
     8941 ggaccatcat ttaccagttt aactttcata tcggctgcaa aaacgccggt tttaacctct
     9001 ttaccggttt tttgttccat tattttacaa aaactttcgt aaagcggaat ggctgtttca
     9061 tgccgtgcag cccgaatata agaaggtcgg ttccccttcc tggtttctgc acaaagtgta
     9121 aactgactca ccaccaacac ttctccatcg atatccgtaa cggaaagatt cataactcct
     9181 ttttcgtcgt caaaaatacg taatgctgcc gttttaccgg ccaaccattc agcatcctgc
     9241 aaagtatcgg tgctttctat accaacgaag accaacaatc cttttcctat ggaagatatt
     9301 atttgtcgat caatttctac cgaagcttct gctactcgct gaattaaaac gcgcatgaca
     9361 aattttgctt tttaaggcaa tttgaagcaa aaatagatta aactcaaaca ttgaatcgga
     9421 aatgcataat atcgccatcc tgcaccacat aatccctgcc ttcgatataa atctttcctg
     9481 cttctctgca agccgtttcc gagcctaagg ccacaaaatc ctcatacttg attacttcgg
     9541 cacgaataaa gcctttttca aaatcggtat gaattactcc ggcacattgc ggagctttcc
     9601 atcctttttc gtaagtccat gcccgaactt ccagtggccc gacagtaaaa aatgtttcca
     9661 aattgagcaa acgataagcc gattgaatca agcgggatac tcccgattcc tttaaaccaa
     9721 gttcctccaa aaacatctga cgctcttcat acgattccag ctcggcaatt tcggcttcag
     9781 ttgcggcagc caccaccaat atttcggcat tctcttcctt tactgcttct ctcactgcct
     9841 ccacataagc atttccgctt gttgcagaag cttcgtccac attacagata tacattacag
     9901 gcttgtcggt caataaaaaa agatctttga ccaacttttt agattctttg tccaacacta
     9961 cggtacgtgc cgattttccc tgtaacaaag ctgaccgata ttcctgcaaa atttcataaa
    10021 ttattttcgc ctgcttgtcg ccaccggttt gggcctgttt ctgcacttta tttattcggc
    10081 tttctactgt ttccaaatct ttcaactgca actccgcatc tactatttcc ttgtcgcgaa
    10141 ccgggtcaat aactccattg acgtgggcca cattttcatc ttcaaaacac cgcaatacat
    10201 gcagaatggc atctgtttca cgaatattgg ccaaaaattt gtttcccaag ccctctcctt
    10261 tactcgcacc ttttaccagt ccggcaatat ccacaatttc caccgtagcc ggaactaatc
    10321 tttgcggatt tgccaatcgg gccaagatat taatcctttc gtcgggaact tgcgtaacgc
    10381 caacattggg ttcaatggta caaaaaggaa aattggccgc ttgagccttt gcattcgaca
    10441 gacagttgaa taatgtggat ttgccaacat tcggcaaacc tacaatacca cattgaagtg
    10501 ccatataatt atccgatcaa ttaaattatt cttctttttt aaaactattt ctttgtacaa
    10561 aagtacatga taattttcaa tttacacacc gactatttaa aaacaaactt tcccgattca
    10621 taaaatcaaa cctataaaac tcatttcagt aatcatgcaa atgaatgaaa atgaaaaata
    10681 agctgaaact tcttcaggaa cgatctgaaa attgaacgga gaaatttatt tccatatttc
    10741 ggcaatgatt cggggaaaaa actcgtattc gagagcatgt accctttgag caaccgattc
    10801 gggagtatcg tcgggcaaca cgggacattt tgcctgaaaa ataatatcgc cttcgtcgta
    10861 atttgaattg acatagtgaa tggtaatacc cgtctccttt tctcctgctt ccaacacggc
    10921 acgatgcaca ttcatgccat acataccttt ccctccaaac tttggcaaca aagcaggatg
    10981 aatattgaca atcttttgcg gataagcttc gataatcgaa tcgggaattt tcagtaaaaa
    11041 tccggacaaa acaatcgcat caatattttt ctcctgaagt aaattcaaaa tttttcctcc
    11101 atcgataaaa tcttcccgcg gaaaagtaag cgaaggaaca cccagttttt ttgcccgctc
    11161 atgaacaaaa gctttctcgt tgttggaaat aatgagagga aaagaaaaat cggaattgcc
    11221 tgtaaaataa cgaattatat tctcagcatt ggaacctgaa cccgaagcaa acaaggcgat
    11281 attttttgac ataattggtg aagaaaaaat gctcaaaaac agctgatttg tgcaaaaaaa
    11341 catatttttt tctaaaaagt gatgattatt tgaagaaaat ttcatttctt tgcaccgcaa
    11401 aattataaaa caatttttat ttataaactt aaaacttaaa attatgtcag aagttgaaga
    11461 aaaagtaaag actattattg cagacaaatt gggtgttgat gaagcagaaa ttacacctac
    11521 ggcaagcttt accaacgatc ttggcgctga ttcgcttgat acggttgaat tgattatgga
    11581 atttgaaaaa gaattcggta tttctattcc tgacgatcaa gctgaaaaaa tcaccactgt
    11641 tggcgatgct gtaagctata tcgaaaatgc taaaaaataa cattccttat ttgttatgga
    11701 attaaaaaga gtagttgtta cagggctggg agccattact ccattgggtt catctgttcc
    11761 caaactgtgg gaaaatatcc tcaatggggt aagtggagct ggtcccatca ctcacttcga
    11821 tgcttctcaa tttaaaacgc aatttgcttg cgaagtcaag gattttgatc cttttcagta
    11881 tttcgaaaaa aaggaaatca gaaaagtcga tccttatgct cagtatgcta tcgcagctgc
    11941 caagcaagct attgaaaact gcggaataaa tttggaaact gtcgacaagg atgaagttgg
    12001 cgtcatcttt tcgtcgggaa ttggcggaat cactactttt gagcaggaag tttcaaacta
    12061 ttttctcaac aaggaaatgg gcccgaaatt taacccgttt tttatcccga agatgatttc
    12121 agatattgca gcaggtcaga tttccattct ttatggtttt aaaggcccaa attatgctac
    12181 aacctcagct tgtgcttcat ctactcattc aattatcgat gccttcaatt acatccgttt
    12241 aggcaaagca aaaatgtttg ttgttggagg agccgaagca gccatcacag ctgccggagt
    12301 tggcggattc aattccatga tggcactatc tacccgaaat gatgatcctg caggagcttc
    12361 acgccctttc agcaaaagca gagatggttt tgtcattggc gaaggagccg gttgcatgat
    12421 tctggaagaa ttggatcatg ctttggctcg tggagcaaat atactttgtg aggtggcagg
    12481 cggcggcatg tcggctgatg cttaccattt gacagcttca caccctgaag gacttggcgc
    12541 tatcctcgcc atgaagagtg cactcaaaga tgcaggaatg caagttgaag acatcgacta
    12601 cattaatgtt catggtactt ctacgcccgt tggcgacaca tccgaagcaa aagccattga
    12661 aaacgttttt ggagaacagg catatcaact gaatatcagt tcaaccaaat cgatgacagg
    12721 ccatttgctg ggcgctgccg gtgctgttga agccattatc tgtacgcttg ccattattaa
    12781 tgatgtggtt ccgcccacca ttaatttcac ggaaggcgat gaagaccccg atattaatta
    12841 ccggttaaac tttaccttca ataagccaca gaaaagaacc attaatgcag ctttatccaa
    12901 caactttggt tttggtggac ataatgcaag tgttatatta aagaaattca ccaaataagt
    12961 tcgtgaaaaa tttctttcat ttgaatgggc tcttttccaa atacagaaaa gagccttatt
    13021 ctttgttccg aaaactattg ggcttttatc cgcgcgatat caactattac ctgctggctc
    13081 tgacacatcg ctccttgtca atgacgaacc atgatgggaa agttgtgagc aacgaaagac
    13141 ttgagttttt gggtgatgcc gtattgaacg ctatcgtttc cgatttattt taccggtatt
    13201 atgagcaaaa agatgagggg tttctcacca atattcgttc caaaattgtc aatcgcgatt
    13261 cgttgaatca cttggcactg aaacttgaat tggacaaaat tgtgaaagtt tccaaaaatg
    13321 tgaatccgca agtcagcaac gatatttacg gaaatgcttt ggaagcttta attggcgcaa
    13381 tttatctcga ttacggatac aaccactgta tgaagtttgt caaaacaaaa ctttttgagc
    13441 cttttatcga tttgtcaaaa gtagaaaaat tcgagatgaa ctataaatcg aaattaattg
    13501 aatggtgtca gaaaaatcac ttccaaatcg acttcgagat gtcctgcaaa actcacaacg
    13561 aaaaccatca accaattttt ctgtcgcgtg tactcattga aggaaggcct gtttccgaat
    13621 caacgggact gaacaagaaa gaatctcaac aaaaagcctc tcgcatcgcc tatcaacaaa
    13681 ttatcgagca gaaaacattt cattttataa acgaagaatc aaaaaattag tctgcctcaa
    13741 ctcagcaaaa cttaagctga aaaagctact aaaaggaaat tccttttcag caacagatta
    13801 aattaatttt ttggacaatt gaaaactttt aactaatttt gtaggtgata taaatttttt
    13861 gtataactaa aaaccaaacc gctatgtcaa cagaaaacat cggaattaat gccggagccg
    13921 tatggaaagt attgaacgaa aaaggagaaa tggacgttaa agcgcttaaa aaaagcgcca
    13981 aactctctga aaaagatttg aatatggcac taggctggct tgcccgagaa ggaaaaattg
    14041 aatttaacga aacagaggaa aatttattgg ttcacctcgt ttgagagagt gaaatataaa
    14101 aaaggcggaa tggaaatttc gccttttttc aaattttaag gaatcctgct tcacttttca
    14161 agccaaaaaa tttcgtaaag cgaaaaaatg cttgtatatt tgcacccgct ttacgcatga
    14221 aaatgttaga tatgcgaaaa tagctcagtt ggtagagcac gaccttgcca aggtcggggt
    14281 cgcgggttcg agtcccgttt ttcgctcatt gaaaaagtct tttacttttt tgcccggatg
    14341 gtggaatggt agacacgaag gacttaaaat cctttggccc caaaggctgt gcgggttcaa
    14401 gtcccgcttc gggtaccaac aaacccctta ctcatcttta agatttgcaa ggggtttgtt
    14461 atttttttgc aaattcctaa tgaatttgct tgggctcacc tgcaaaaaat atgtgtttgt
    14521 agaaaaagaa aagtatcttt gtgaaaaaat actttttata tgtccactta
//
LOCUS       DAOSCH010000050        14062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2933_length_14062_cov_5.738702, whole genome shotgun sequence.
ACCESSION   DAOSCH010000050 DAOSCH010000000
VERSION     DAOSCH010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 14062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14062
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2933_length_14062_cov_5.738702"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..172)
                     /locus_tag="PLZ54_05280"
     tRNA            complement(98..172)
                     /locus_tag="PLZ54_05280"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(135..137),aa:Pro,seq:tgg)
     gene            293..1420
                     /gene="hemW"
                     /locus_tag="PLZ54_05285"
     CDS             293..1420
                     /gene="hemW"
                     /locus_tag="PLZ54_05285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOJ66374.1"
                     /translation="MAGIYIHIPFCIQRCSYCNFFSVVSINLVNEVINAIIKELSIRK
                     DYLKNEPVQTIYFGGGTPSLLTIQQIEKLLNTVYTNFPVEQNAEITFEANPDDLTKNY
                     LFDLKEVGINRLSIGIQSFDDDKLKILNRRHNATQAHEAVREAQKAGFSNISIDLIYG
                     LPFQSLDEWETDLDKAFSLSIQHLSAYGLTFEEKTLLSKQKEKKIIEPADEELMIKMY
                     ELLLEESSKKGFDAYEISNFSLPGFYSRHNSSYWNQTPYLGVGPSAHSYNGISRQWNV
                     AAIQKYTEGILKNSSFYEQEILSLEDRYNEYVLLGLRTKNGINIDALEQNFGTELKDF
                     CLENIKTFIDNEKAYRCGSFLRLNTKGKLISDTIIAQLMKV"
     gene            1472..3790
                     /locus_tag="PLZ54_05290"
     CDS             1472..3790
                     /locus_tag="PLZ54_05290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOJ66375.1"
                     /translation="MENQNNDKRTKFKKIFWNLFGFGILCLVLLFAFISFGWIGYLPP
                     LEELQNPQNKYASEIYSSDMQLLGRFFKNKENRVEVHFNDISKNVINALIATEDKRFY
                     KHSGIDGKALARAILLTGILQQKSAGGGSTITQQLAKQLYSPQADNIFERMLQKPIEW
                     VISVKLERNYTKEEILTMYLNQFDFLYNAVGIKSAAQIYFSTSPSQLKIEEAATLIGM
                     CKNPAYFNPVRYNERTRDRRNMVLYQMYKCDYLTKQQYDSLKNLPLVLHFQRIDHKEG
                     LAPYFREYLRTTLMAKEPHRSDYASWQDQQYLDDRWAWDNDPLYGFCNKNLKSDGTPY
                     NIYTDGLKIYTTIDSRMQRYAEEAVNEHIQTLQAAFFKEKKNRSYAPFSSSLSPKEIE
                     DILNRSIRQSERYSKMKAQGKSDAEIMEEFKKPVEMRVFSYDGDIDTIMSPLDSIRYH
                     KHFLRCGFMSMEGRSGFVRAYVGGPNFNRFQYDMVNKGRRQVGSTIKPFLYTLAMEEG
                     MWPCDQTVNQPITFRTATGELWTPRNSSKARIGETVTLRWGLANSNNWISAYLMSLFT
                     PESLVKLMRSFGIRGHIDPVVSLFLGPCEISVEEMVDAYTAFMNKGIRVEPVYVTHIE
                     DANGNVIGKFSPKMHEIFSETTAYKMIYMLQGVIDGGTGSRIRTRFGLTMPMGGKTGT
                     TQNNSDGWFIGFTPSLASGVWVGGEERSIHFDRMSEGQGASMALPIWALYMKKVLADS
                     TLNYSPRENFEIPSWFNPNAGCEDSSNGNSNE"
     gene            3783..5837
                     /locus_tag="PLZ54_05295"
     CDS             3783..5837
                     /locus_tag="PLZ54_05295"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ66376.1"
                     /translation="MNNNRFVHFRKKFLLCFTGLLFSFAAHAQWNTDRIMDIGKNAFY
                     FEDYVLSIQYFNHVIKIKPYLSEPYIYRAMAKIQLGDYQGADEDCTQAIEKNPFVPQA
                     YYARGFARLKMNFCNEAIDDFSKALEFSPGNIYLLGYRLMARNQCNDYAGALNDVNEY
                     LKLSPKSTEMYYEKGRILLALKDTVGAEQCFDKYIQLNENSSLGWSARALLKMQKKDK
                     KGALKDYDEAIKRNSTYSGDYINRGVLNTENKNFRQALEDYDKAISLDKNNELAYFNR
                     GLLRASLGDKNNALADFTKVLELNPSNTDALWQKATVENALGNYSQSIKDFSKIIEQY
                     PYFIPAYSGIAEAYQKLGDEKNAFRYRQMAYNIEQNKNNIRKEELAAGNKIAKETQKN
                     VSEKNLDFFNRFAVQDKNDTAGESKYNNNIRGAVQNRYADVVNEKNFVLTYYSKADEI
                     RRTNLFHLSIERYNSQKKLPLPLKITNNELPLTAEMISQHFDAINNISAQLEQNSSDA
                     DIYFARAIEFALVQDFKSAIDDLNKSISIKPDFMPAYFTRANIRYKMIETNSNGNLGG
                     SANKPVNDIGSNNQLKFDMELILRDYDKVIELVPDFTFAYYNKANVLTAIRDFRSAVS
                     YYSKAIDIDPDFAEAYFNRGLTYLFIGDDKNGLSDLSKAGELGIYKSYNLIQRFKQQK
                     EN"
     gene            complement(5834..6760)
                     /locus_tag="PLZ54_05300"
     CDS             complement(5834..6760)
                     /locus_tag="PLZ54_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-inducible genes activator"
                     /protein_id="HOJ66377.1"
                     /translation="MTLQQLEYILAVYKTGHFMKAAELCQVTQPTLSAMIQKLEEELD
                     CKIFDRSQHPIQPTEMGEKIIAQAQIILYQTNQLKEMLRKEKETVSGNLNLAIIPTIA
                     SYLLPRFIALFRNDYPEVSLKITEMRTDTIIQKLKTAEIDMAILATPLNNPDLLEIPV
                     YYEKFIAYISPGEPIYELEELSANNLPLEHLWVLEEGHCFRSQIFNFCDQMKVHNYMY
                     EAGSIETLVKIVDINGGYTVIPELHVESLTEEQKKNLRNIVQPEATREISIVIRNDYV
                     REGLLNAVADSLKKIIPPHMLDSRLKKFAIKI"
     gene            complement(6940..7782)
                     /locus_tag="PLZ54_05305"
     CDS             complement(6940..7782)
                     /locus_tag="PLZ54_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ66378.1"
                     /translation="MDNITETVIKPKSSLLEIDFKELWRNRDLFSMFVKRNIVTQYKQ
                     TILGPAWFFIQPALTTIMYMVVFGGIAGIPTDGLPQPMFYLAGIVCWQYFADCLTKTS
                     STFIDNQNIFGKVYFPRLIVPLSTVASNLVRMFIQFLLFVAVYVFYLIKGVHVEPNIY
                     ILLVPVLIIMLAGLSLGFGIIISSLTTKYRDLTILFNFIVQLWMYATPIIYPLSTMPP
                     DRQWIMALNPLTSIIEAFKYGTMGVGTFSWGQLAYSFGFMVVLLAVGIVIFNKVQRTF
                     MDTI"
     gene            complement(7828..9162)
                     /locus_tag="PLZ54_05310"
     CDS             complement(7828..9162)
                     /locus_tag="PLZ54_05310"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOJ66379.1"
                     /translation="MEIAIVGTGYVGLVTGTCFAEMGIDVTCVDIDEQKIEKLKSGII
                     PIYEPGLGDMVLHNVKAGRLHFTTKLTDVLDDVQVIFSAVGTPPGEDGSADLRYVLDV
                     ARTIGQNMNQYVLLVTKSTVPVGTAKLVRQTIQNELDKRGVSIEFDVASNPEFLKEGD
                     AIKDFMSPDRVIVGVESEKAREIFTRLYRPFLINNFRVIFMDIPSAEMTKYAANSMLA
                     TRISFMNDIANLCEIVGADVNMVRKGIGTDQRIGSKFLYAGCGYGGSCFPKDIKALIQ
                     TAREKGYEMKVLKAVEEVNNTQKEILYRKLIKHLGENLAGKTIAIWGLSFKPNTDDVR
                     EAPALLLTEKLAKAGCHVRVYDPVAMPEAKTALSKILPEFLDKIHFASDIYDAALESE
                     ALLLVTEWKEFRMPSWGVIRKAMKNPLILDGRNIYDKKELQELGFTYEGIGV"
     gene            complement(9202..10263)
                     /locus_tag="PLZ54_05315"
     CDS             complement(9202..10263)
                     /locus_tag="PLZ54_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOJ66380.1"
                     /translation="MDKNSKIYIAGHKGMVGSAIMRNLQNKGFSNIVGRTREELDLLN
                     QISVARFFDEEQPEYVVLAAAKVGGIVANNTLRAQFIYENLMIQNHVIHQSYLHGIKK
                     LLFLGSSCIYPRLAKQPIKEEYLLTGELEPTNEPYAIAKIAGIKMCEAYHAQYGCNFI
                     SMMPTNLYGPNDNYDLEKSHVLPALIRKMLLGKYLMENDFESLRKDFQKHPIEGVNGT
                     YSEEILLKVLEKYGISRKGQEVSITLWGTGSPLREFLHVDDMADAAVHLFFHYDAPDT
                     VPSHVNIGCGEDIRIKELAEIIKKIVGYEGKIVWDASKPDGTPRKLLDVSKLNALGWN
                     PKITLEDGIRQVVNNYVFS"
     gene            complement(10278..11432)
                     /gene="gmd"
                     /locus_tag="PLZ54_05320"
     CDS             complement(10278..11432)
                     /gene="gmd"
                     /locus_tag="PLZ54_05320"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOJ66381.1"
                     /translation="MENKKVALITGITGQDGAYLADFLLKKGYLVHGIKRRSSLFNTD
                     RIDHIYQDPHEQNRNFVLHYGDMTDSLNLTRIIQETQPDEIYNLAAMSHVKVSFDTPE
                     YTANADGIGTLRLLEAIRLLGLTEKTKFYQASTSELYGLVQETPQTEKTPFYPRSPYA
                     VAKLYGYWITVNYREAYGIHASNGILFNHESPLRGETFVTRKITRAASRIALGLQKNL
                     FLGNLSSKRDWGHARDYVKAMYLILQQDQPDDYVIATGKTTEVREFVRLAFEQMGLTI
                     AFEGKDLNEKGYIVQIDEPLFVQKIGEEFLSLVKSLKHPVIEVDPTYFRPTEVDLLMG
                     DATKARTKLNWQPEYDLEALIKDMMQSDIKLMKKELHLKNGGFKVLNYFE"
     gene            complement(11472..13847)
                     /locus_tag="PLZ54_05325"
     CDS             complement(11472..13847)
                     /locus_tag="PLZ54_05325"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOJ66382.1"
                     /translation="MNPDTGSINQNFTEEESSFDFQKWLHLFIKNWYLFLIFIPFFLI
                     GGYLKNRSWMPLYQSVGTIIINENKYRSSSQGFMQGFGLEEAYQNVNNQVIILNSYDL
                     IGKVVDSLPFMKVDYLTIGRFKTRNLYTTTPIIIETDYVAPEAYELLFKISLHPNGTF
                     TISPHNNNSMPSSPINGHIGVPLQHNLFFMTVNSVSDNVKEFEMYFRFRTRETLIEDF
                     KSRLSSRYVIEGSSILEISLISETPDRDVDFINKLSEIYLEENLKKKNDVASKTIQFI
                     NEQLSEVSKSLSVSEDEMTRFRRDNQIIDISSHGSELMSKANSLDEKKAELKLKETYL
                     NYLTKYLTGNFEEGTVVAPSNLGLNEPLLMQLVQQINELYLQRSELTEKNLYYAKYTK
                     AIENVKTSIFELIKNMRASLEIEKNEVNRLLADVNSQIAELPKKELEIIGIERNYKVN
                     DNYYTFFLQKRAEAEIQKASNTPDNNILDQARTVSLINADVKSNTMFMFILLGILIPS
                     LIIVLKEVLNNTIRSIKDIEKRSVFPVIGMIRHAKNDLPDLTLKNPKSSFTEMFRVIR
                     TRVEFVVRRKTDIVMMVTSVDAGDGKTFFSTNMAGVYGMTGHKTLLIDMDIRKPGINR
                     RFNVNHPKGVTNYLINECTLDEIINVVPEVNYDVIFAGTVPPNPAELIRSEKIIEMMG
                     ELRKRYDYIILDSSPIGLVTDSFSLASLADVNLLVVRNNKTHKDFFKNVVNQIVTDKL
                     SNFYVVFTDVETDGNYYSYKYGYSSKYGYYTSKGQKNIDEYRKYYDDNNEI"
     gene            complement(13882..>14062)
                     /locus_tag="PLZ54_05330"
     CDS             complement(13882..>14062)
                     /locus_tag="PLZ54_05330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66383.1"
                     /translation="DIRPASVIESKYYYVYPNDIIYVQRASSSFFKMNNYSSFLGLIS
                     SSLTLLFSVLYYIKL"
BASE COUNT         4482 a         2767 c         2535 g         4278 t
ORIGIN      
        1 attgtgtcgg ggtagcggga tttgaaccca cgaccctcca gcctttggct ggatgcgcta
       61 accttgtaaa ttttttaaat atcgttcttc tattgtgtcg gggtagcggg atttgaaccc
      121 acgaccccct gctcccaaag caggtgcgct aaccggactg cgctacaccc cgtactgatt
      181 tttttaaaaa cgactgcaaa gttaaaatct tttgccgaaa ataccaactt tttgctgtat
      241 ttttgtataa aagaattctt gaattgttcc taatttcttt cccgctttaa aaatggccgg
      301 tatttacatt catattcctt tttgcatcca gcgatgttcc tattgcaatt ttttttccgt
      361 agtctctatc aatcttgtta atgaagttat aaatgcaata attaaagaat tgtcgatcag
      421 aaaagactat ttgaaaaatg agccggtaca gacgatttat tttggcggag gaacacccag
      481 tctgttgacg attcaacaaa ttgaaaaatt actgaatact gtttatacaa atttcccggt
      541 agaacaaaac gcagaaataa cgtttgaagc caatcctgat gatttaacaa aaaattattt
      601 gttcgacctg aaagaggtag gtatcaatcg gttaagtatt ggaattcaat catttgatga
      661 cgataaactt aaaatactca atagaagaca caatgcgaca caggctcatg aagcagttcg
      721 tgaagctcaa aaggcaggtt ttagcaatat cagcatagat ttaatatatg gattaccctt
      781 ccaaagcctc gatgaatggg aaactgattt ggataaagct ttttcactgt ccattcaaca
      841 tctttctgcc tatggcctaa cctttgaaga aaaaacactt ttatcgaaac aaaaagaaaa
      901 aaaaatcata gaaccggcag acgaagaatt gatgataaaa atgtatgaac ttttgttgga
      961 agaaagcagc aaaaaggggt ttgatgctta cgaaatatcc aatttttcct taccgggatt
     1021 ttattcacga cataattctt cctattggaa tcaaactcca tatttgggcg taggaccttc
     1081 cgcacattcc tataatggca tttctcggca gtggaatgtt gctgctattc agaaatatac
     1141 agaaggaatt ttgaaaaaca gttcctttta cgaacaggaa attctcagtt tggaagatcg
     1201 ttataatgaa tatgtattac tcggattgag aacaaaaaac ggtataaata ttgatgcgtt
     1261 ggaacaaaat tttggtaccg aattgaaaga cttttgttta gaaaacataa aaacatttat
     1321 cgataatgaa aaggcatata ggtgcggcag ttttttacgt ttaaacacaa aaggaaaact
     1381 aatatccgac accattatcg ctcaattaat gaaggtttaa gttatggaaa attattacta
     1441 attttgtaac ttattcgtta gattaaaaaa tatggagaat cagaacaacg acaagcgtac
     1501 taaatttaaa aaaattttct ggaatttatt tggatttggc atcctttgct tggttttgct
     1561 ctttgctttc attagttttg gctggattgg ttacttgcct ccactcgaag aattacaaaa
     1621 tcctcaaaac aaatatgctt cggaaattta ctcttcagat atgcaattgc tgggtagatt
     1681 ttttaaaaac aaggaaaatc gtgtagaagt acatttcaac gacatttcta aaaatgttat
     1741 taatgctttg attgctactg aagacaaaag attttacaaa cattccggca tcgacggcaa
     1801 agcgcttgcc cgagctattt tacttaccgg tattttgcag caaaaaagcg ccggaggagg
     1861 aagcaccatt acccaacaat tggccaagca actttattca cctcaagccg acaacatttt
     1921 tgaacgaatg cttcaaaaac ccattgaatg ggtaatatct gtcaaactgg aacggaacta
     1981 tacaaaagaa gaaattctga ccatgtacct taatcagttt gatttccttt acaatgctgt
     2041 aggaataaaa tcggctgctc aaatctattt cagcaccagt ccttctcaac tgaaaattga
     2101 agaagctgcc actttgatag gaatgtgcaa aaatcccgct tactttaatc ccgtacgtta
     2161 caatgaacgt acacgcgaca ggcgcaatat ggttttatat caaatgtaca aatgtgatta
     2221 tctcacaaaa caacagtatg attcattaaa aaatcttccg ttggtgcttc attttcagcg
     2281 aatcgatcac aaagaaggat tggcccccta tttcagagag tacctcagaa ccacattaat
     2341 ggcaaaagaa cctcatcgaa gcgattatgc cagctggcaa gaccaacaat atctcgatga
     2401 tcgctgggct tgggacaatg accctttgta tggattctgc aataaaaatc tgaaatcaga
     2461 cggaacgccg tacaatatct atactgatgg tttgaaaatt tataccacca tcgattctcg
     2521 tatgcagcga tatgcagaag aggctgtaaa tgaacacatt caaacattgc aggctgcatt
     2581 tttcaaagaa aagaaaaaca gaagttatgc tcctttctcc agtagcctga gccccaaaga
     2641 aatagaagat attttgaatc gctcgatccg gcaatcggaa agatattcta aaatgaaagc
     2701 acaaggcaaa agcgatgccg aaattatgga agaattcaag aaaccggtag aaatgagagt
     2761 tttctcttat gatggtgata tcgacaccat tatgtctcca ctcgattcca ttcgctacca
     2821 caaacatttc ttgcgttgtg gtttcatgtc gatggaagga cgcagcggat ttgtcagagc
     2881 ttatgttggt ggcccgaatt tcaatcggtt ccaatacgat atggtcaaca aaggacgccg
     2941 acaagtgggt tcaaccatca aaccgttcct ttacacattg gcaatggaag aaggaatgtg
     3001 gccttgcgat caaacagtta atcagcccat tactttcaga acagccaccg gtgaactttg
     3061 gacaccaaga aactcttcaa aagccagaat tggagaaaca gttaccctgc gttggggact
     3121 tgccaattca aacaactgga tttcagctta tttaatgagt cttttcactc ccgaatcgct
     3181 tgtaaaactt atgcggtcct ttggcattag aggtcatatt gatcccgttg tatcattgtt
     3241 tttgggacct tgcgaaattt ccgttgaaga aatggtggat gcctatactg catttatgaa
     3301 taaaggaatt agagtggaac cggtttatgt aactcacatt gaagatgcca acggaaatgt
     3361 aattggaaaa ttttctccga aaatgcatga aattttcagt gaaactacgg cttacaaaat
     3421 gatttacatg ctacagggag ttattgacgg aggaaccggt tcgagaatca gaacacgttt
     3481 cggattaact atgccgatgg gaggaaaaac gggaacgaca caaaacaatt cggacggttg
     3541 gttcatcgga tttactcctt cactggcttc gggggtttgg gttggtggtg aagaacgttc
     3601 cattcatttc gacagaatgt cggaagggca aggtgcaagt atggctttgc caatttgggc
     3661 tttgtacatg aaaaaagttt tggctgattc aactttaaac tattcaccca gagaaaattt
     3721 tgaaattcct tcctggttca atcccaatgc cggttgtgaa gatagcagta atggaaatag
     3781 taatgaataa taatagattc gtccacttca gaaaaaaatt tcttctgtgc ttcacaggat
     3841 tattattttc ctttgccgcc catgctcaat ggaataccga tagaattatg gacataggga
     3901 aaaatgcttt ttattttgaa gattatgtac tttccattca gtatttcaat catgtaatca
     3961 aaattaagcc gtatttgtcg gaaccttata tttatcgggc aatggcaaaa attcaactcg
     4021 gcgattatca gggcgccgac gaagactgta cgcaagctat tgaaaaaaat ccttttgttc
     4081 cgcaagccta ctatgccaga ggtttcgccc gattgaaaat gaatttttgt aacgaagcta
     4141 tcgatgattt ctcgaaagcg ctggaattta gtccaggaaa tatatatttg cttggctatc
     4201 ggcttatggc acgcaaccaa tgtaatgatt acgcaggtgc tttgaatgat gtaaacgagt
     4261 atctgaaatt aagccccaaa agtacggaaa tgtattatga aaagggcaga attctgttgg
     4321 cattaaaaga cacggttgga gccgagcaat gtttcgacaa atacattcag ttgaacgaaa
     4381 atagcagctt ggggtggagt gctcgtgctt tattgaaaat gcagaaaaaa gacaaaaaag
     4441 gagctttgaa agattatgat gaagccatca agagaaattc aacctacagc ggagattaca
     4501 ttaacagagg cgtattaaac acggaaaaca aaaatttcag gcaagctctg gaagattacg
     4561 ataaagcaat ttcactggac aaaaacaatg aattggctta tttcaacaga ggtttactca
     4621 gagccagtct tggagataaa aataatgcac ttgcagattt tacaaaagtt ttggaactta
     4681 atccatccaa taccgacgct ctctggcaaa aagctacagt agaaaatgca ctgggaaatt
     4741 actcgcagtc cataaaagat ttttcaaaaa ttatcgaaca atatccttat tttatcccgg
     4801 cctactctgg aatagccgaa gcctatcaga aacttggtga tgagaaaaat gccttcagat
     4861 acaggcaaat ggcttataac atagaacaaa acaaaaataa catcaggaag gaagaattgg
     4921 ctgccggcaa taaaattgca aaagaaaccc agaaaaatgt ttctgaaaag aatctcgatt
     4981 tcttcaatcg ttttgctgtg caggataaaa atgatactgc cggcgaatca aaatataaca
     5041 acaacattcg tggagcagta caaaaccgat atgcagacgt tgtgaacgaa aagaattttg
     5101 tgctgactta ttacagtaaa gctgatgaaa ttcgcagaac caatcttttc catctgtcca
     5161 ttgaacgcta caacagccag aaaaaactgc cgctgccctt aaaaattacc aataacgaat
     5221 tacccctgac agccgagatg attagtcagc atttcgatgc tatcaacaat atttctgcgc
     5281 aactcgaaca aaactcatcg gatgcagata tttattttgc cagagccatt gaatttgctt
     5341 tggtacagga ttttaaaagc gctattgatg atttgaacaa atccatttcg atcaagcccg
     5401 actttatgcc ggcttatttt acccgagcaa atattcgata caaaatgatt gaaacaaatt
     5461 ccaatggaaa tttgggtggt tctgccaaca aaccggttaa cgacattggt tcgaataatc
     5521 aattgaaatt tgatatggaa ttaattctga gagattatga taaggtcata gaattggttc
     5581 ccgatttcac atttgcttat tacaacaaag ccaatgtttt aactgccatt cgcgattttc
     5641 gttcggctgt aagttactac agcaaagcca tagatattga tcccgacttt gcagaagcct
     5701 attttaatcg gggattaacc tatctgttta tcggagacga caaaaacgga ctgtcggatt
     5761 tgagcaaagc cggagaattg ggaatttata aaagctataa tctgattcaa agattcaagc
     5821 agcagaaaga aaactaaatt ttaatggcaa attttttcaa gcggctatcc agcatgtgag
     5881 gaggtattat tttcttcagg ctgtcagcaa cggcattcaa caagccttcg cgaacataat
     5941 cgtttctaat gacgatggaa atttctctgg tggcttcggg ttgaacgata tttcgaagat
     6001 tctttttctg ctcttcggta agtgactcaa catgtaattc gggaattacc gtataacctc
     6061 cgttgatatc cacaattttc accaatgttt caatactgcc ggcttcatac atgtaattat
     6121 gaactttcat ttggtcgcag aaattgaaaa tctgacttct gaaacaatgc ccttcttcca
     6181 gcacccaaag atgctccaac ggcaaattgt tggccgacaa ttcttctaac tcataaattg
     6241 gttcgccggg agaaatatag gcaataaatt tttcgtaata aacaggaatt tcgagcaagt
     6301 caggattgtt aagtggcgtt gccaatatcg ccatatcaat ttcggcagtt ttcaacttct
     6361 gaataatggt gtcggtacgc atttcagtta ttttcaacga gacttccgga taatcgtttc
     6421 tgaacaaggc aataaaacgc ggcaaaagat aggaagcaat agttggaata attgccagat
     6481 tcaaatttcc cgaaacagtt tccttttctt tccgtaacat ttctttcaac tgattggtct
     6541 gataaagaat aatttgagcc tgagcaataa ttttttctcc catttcggtg ggctgaatgg
     6601 gatgctgcga gcggtcaaat atcttgcagt caagttcttc ttccaatttt tgaatcatgg
     6661 cgctcaaagt tggctgagtt acctgacaca attcggcagc tttcataaaa tgacccgttt
     6721 tatatactgc cagaatatat tccaattgct gtaaagtcat gtcgagaaat tattaaatta
     6781 acgtgagttc gatataaaga ccttatctaa acaaaactaa tctcagtaat aggggaattt
     6841 tccccttttt catcttcctt ttaactaatt tttaattact tataaaacat tcttatacct
     6901 aactcaattt taaccaatta aattagcttt gatttatttc taaatagtgt ccatgaatgt
     6961 tcgctgcact ttgttgaaaa taacaattcc aaccgccaga agaacaacca taaaaccaaa
     7021 actgtaagcc aattgccccc agctgaaagt tcctacgccc atagttccgt acttgaaagc
     7081 ttcaataatg gatgtcagcg gattcaatgc cataatccat tgacgatcgg gcggcatggt
     7141 gcttaaagga taaataattg gcgtagcata catccacaac tgtacaataa agttgaacaa
     7201 aatagttaaa tcgcgatact tggtggtaag tgaagaaata attatgccaa agccaagtga
     7261 taagccggcc aacatgataa taagcaccgg aacgagcaat atataaatat tcggctcaac
     7321 atgaacgcct ttaatcaaat aaaaaacata tacggcaaca aaaagcaaaa actgaataaa
     7381 catccgcaca aggttggaag cgacggtaga aagtggaaca atcagtcgtg gaaaataaac
     7441 ttttccgaaa atattctgat tgtcgataaa agtactggaa gttttggtca aacaatcggc
     7501 aaaatactgc cagcaaacaa tgccggctaa ataaaacatg ggctgtggta atccatcggt
     7561 cgggatgccg gcaataccgc caaaaacaac catgtacatg atggttgtca atgccggttg
     7621 aatgaaaaac cacgcagggc ccaaaatggt ttgcttgtat tgcgttacga tattgcgctt
     7681 aacaaacatg gaaaataaat ccctatttct ccataattcc ttaaaatcaa tttccaacaa
     7741 actgcttttg ggcttgataa ctgtttcggt aatattatcc atacaatatt ctctcagtaa
     7801 aatcaacaaa ttatgtatat tttgtcatca aactccaata ccttcataag taaatcccaa
     7861 ttcttgaagt tcttttttgt cgtaaatatt tcgtccatca agaatcaaag gatttttcat
     7921 tgctttcctg ataacgcccc aactcggcat tctgaattct ttccattcgg taaccagcaa
     7981 cagagcttcc gattctaaag cagcatcgta aatatcggaa gcaaaatgaa ttttgtcaag
     8041 aaattcaggc aaaattttgc tcaacgctgt ttttgcttcg ggcatggcca ccggatcgta
     8101 aacacgaaca tggcatccgg cttttgccaa tttctctgtc aacaacaacg caggagcttc
     8161 acgaacatca tctgtattgg gcttaaatga taatccccaa atggcaatgg ttttccctgc
     8221 caaattttca cccaaatgct taatcaattt acggtaaaga atttctttct gagtattatt
     8281 tacttcttct acagctttca atactttcat ttcgtatcct ttttcccttg cagtctgtat
     8341 aagagcttta atgtcttttg ggaagcagga accaccgtaa ccacagccgg catacaggaa
     8401 tttactgcca atgcgctgat cggtaccaat accttttctt accatattta catcggcacc
     8461 cacaatttcg caaaggttgg caatatcgtt cataaaactg atacgtgttg ccagcattga
     8521 attagctgca tatttagtca tttcggcgga aggaatatcc ataaaaataa cacgaaaatt
     8581 gttgatcaga aaaggacggt acaagcgggt aaaaatctcg cgtgctttct cgctttccac
     8641 acccacaata actctgtcgg gactcatgaa atcttttatg gcatcacctt ctttcagaaa
     8701 ttcgggattg gaagccacat caaattcaat tgacaccccg cgtttgtcca attcattctg
     8761 gatggtttgg cgaacaagtt ttgctgttcc tacgggaacc gtacttttgg ttaccagcaa
     8821 tacatattga ttcatgtttt gccctatcgt gcgtgccaca tcaagaacat accttaaatc
     8881 ggcacttcca tcttcaccgg gaggtgttcc tacagcactg aaaattacct gaacatcgtc
     8941 cagcacgtcg gttagttttg tagtaaaatg caaacgacca gctttgacat tgtgtaaaac
     9001 catatctccc aatcccggtt cataaattgg aattatgccg cttttcaatt tttctatttt
     9061 ttgttcatca atatccacac acgtaacatc gattcccatt tcggcaaaac aagtaccggt
     9121 aaccaaaccg acgtacccgg ttccaacaat agctatttcc atattatcaa gttaattttt
     9181 tccctattaa aaatgtccga atcatgaaaa aacataattg tttactactt gacgaattcc
     9241 atcttcaaga gtaatttttg ggttccaacc caaagcgttc agtttggaaa catccagtaa
     9301 ttttcgtgga gtaccatccg gttttgatgc atcccaaaca atttttcctt cgtaaccaac
     9361 aattttttta ataatctcag ccaactcttt tattcgaata tcttcaccac aaccaatatt
     9421 gacgtgtgaa ggcacagtat cgggggcatc gtaatggaaa aacaaatgaa cggcagcgtc
     9481 agccatatcg tcaacatgca ggaattcacg aagtggactg ccggttcccc aaagtgtaat
     9541 cgaaacctcc tgacctttgc gtgagatacc gtatttttca agaactttca acagaatttc
     9601 ttcgctataa gtgccattaa ctccctcaat gggatgtttt tgaaaatctt ttctcaaact
     9661 ttcaaaatcg ttttccatca agtattttcc caacagcatt tttcttatca aagccggtaa
     9721 tacgtgcgat ttctcaagat cataattatc attggggccg taaagattgg tgggcatcat
     9781 cgaaataaaa ttacagccat actgagcatg ataagcttca cacattttta tgcctgcaat
     9841 ttttgcaatg gcataaggct cgttggttgg ctccagctca cctgttagca ggtattcttc
     9901 cttaataggt tgtttggcca aacgtggata aatgcacgaa gaaccaagaa acaagagttt
     9961 ttttatccca tgcaggtaac tttgatgaat gacatgattc tgtatcatca aattttcata
    10021 aataaactgg gcacgtaagg tattgttggc cacaattccg cccactttgg cagctgccag
    10081 cacaacatat tccggttgtt cttcatcaaa aaaacgggca accgaaattt gattcaacaa
    10141 atcgagttct tccctggttc gtccaactat attcgaaaat cctttatttt gcaaatttcg
    10201 cataatcgcc gaacctacca tccctttgtg tccggctata tatattttgg aatttttatc
    10261 cattgtaaat tttataatta ttcaaaataa ttcagcactt taaaaccacc attttttaaa
    10321 tgtagctcct ttttcattaa tttaatatcc gactgcatca tgtccttgat caatgcctcc
    10381 aaatcatatt ccggttgcca gttcaatttg gttctggctt tcgttgcatc acccatcaac
    10441 aaatccactt cagtcggacg gaaataggtt ggatcaactt ctatcacagg atgtttaaga
    10501 gattttacta acgacaaaaa ttcttctcca attttttgca caaaaagtgg ctcatctatt
    10561 tgcacaatgt atcctttttc gttcaaatct tttccctcaa atgctatcgt caagcccatc
    10621 tgttcaaaag ccaatcgaac aaattccctg acttctgtgg tttttcccgt tgcaattaca
    10681 taatcatcgg gttgatcctg ctgtaaaatc aaatacattg ctttcacata atcgcgggca
    10741 tgtccccaat cccgtttgga ggacaaattt cccagaaaaa gatttttttg caagcccaat
    10801 gctattctgg atgctgcacg cgtaattttt cgcgtaacaa aagtttcacc acgcaatggc
    10861 gattcatgat tgaacaaaat gccattgctg gcatgaatac cataagcttc tctataattt
    10921 accgtaatcc agtaaccata aagttttgcc acagcatacg gtgaacgggg ataaaaagga
    10981 gtcttttcgg tttgtggagt ttcttgaacc aaaccataca attcggaagt tgaagcctga
    11041 taaaattttg ttttttcggt aagtcccaat aaacgaatag cttccagcaa acgtaaagtt
    11101 cctattccat cggcattggc ggtgtattcc ggtgtatcaa aactcacttt tacatggctc
    11161 atggcagcaa gattgtagat ttcatcaggt tgagtttcct gaataattct ggtcagattc
    11221 aacgaatcgg tcatatcgcc atagtgcaaa acaaaattcc ggttttgttc atgaggatcc
    11281 tgatatatat gatcaattct gtcggtattg aacaatgaag aacgacgttt tattccatga
    11341 accaaatagc cttttttcaa aagaaaatca gccagataag caccatcctg acccgtaata
    11401 cctgtaatta atgctacttt tttgttttcc ataatgctaa tagtttgaca aataaatttt
    11461 ttgtagctta tttatatttc attattatca tcgtagtatt tgcggtattc atcaatgttt
    11521 ttttgtcctt ttgaagtata gtacccatat tttgacgaat atccatattt ataggaatag
    11581 taattgccat ctgtttctac atcggtaaat accacataaa aattgctcaa tttgtcggtt
    11641 acaatctgat tcacaacatt tttgaaaaag tctttatgtg ttttattgtt tcgcaccacc
    11701 agcaaattaa catcggccaa cgatgccagt gagaaactgt cggtaaccaa gcctattggg
    11761 ctcgaatcga gaatgatgta atcgtatcgt ttccgaagtt cacccatcat ttctataatt
    11821 ttttcagacc ggatcaattc agctggattt ggtgggactg taccggcaaa aatcacatcg
    11881 taattaacct caggaacgac gttaataatt tcatccagcg tacattcatt aatcagataa
    11941 ttggtaactc cttttggatg atttacattg aaacgcctgt ttatgcccgg ttttctgatg
    12001 tccatatcaa tcaatagagt tttatgaccg gtcattccat aaactccggc catattggtc
    12061 gagaaaaatg ttttaccgtc gccagcatcc accgaagtaa ccatcatgac tatgtctgtt
    12121 ttgcgacgca ctacaaactc cactcgtgta cgaattaccc tgaacatttc ggtaaaggaa
    12181 gatttgggat ttttcagagt cagatcaggc aagtcgtttt ttgcatgacg aatcatacca
    12241 atcaccggaa acactgatcg cttttcaata tcttttatcg aacgaatggt attgttcaaa
    12301 acttctttta gcacaattat caaagaaggg atcaaaatac caagtaaaat aaacataaac
    12361 atggtattag acttgacatc tgcattaatc agagatacgg tacgggcttg atccagaatg
    12421 ttattgtccg gcgtattgga agccttttga atttcggctt cagctctttt ttgcaggaaa
    12481 aaagtataat aattgtcgtt gaccttgtaa tttctttcaa ttccaataat ttccaattct
    12541 tttttaggga gttcggctat ttggctgtta acgtctgcaa gaaggcgatt gacttcattt
    12601 ttttcgattt ccaacgaagc cctcatattt tttatcaatt caaaaattga agttttcaca
    12661 ttttctatgg ctttggtata cttggcataa tacagatttt tttcggttaa ttcgcttcgt
    12721 tgtaaatata attcgttgat ctgttgaacc aattgcatta acaaaggctc atttagcccg
    12781 agatttgatg gagcaacaac cgttccttct tcaaaattac ccgtgagata tttggtcaaa
    12841 taattcagat aagtttcctt gagttttagt tcggctttct tttcatccaa agaattggcc
    12901 ttgctcatca attcactccc atgactcgaa atatcaatga tttgattatc ccttctgaat
    12961 cgtgtcattt catcttcgga aacagacagg gatttggata cctccgataa ttgttcatta
    13021 ataaattgaa tggttttgga ggcaacatcg tttttctttt tcaaattttc ttcaaggtag
    13081 atttcgctca atttgttgat aaagtccaca tccctgtctg gagtttcact gatcaatgaa
    13141 atttccaaaa tggaagaacc ttcaatgacg tatcttgaac ttaatcgcga tttaaaatct
    13201 tcaatcaatg tttctctggt acgaaagcga aaatacattt caaattcctt aacattgtcg
    13261 ctcacgctgt ttacagtcat aaaaaataaa ttatgctgaa gtggcacgcc tatatgacca
    13321 ttgattggtg aacttggcat gctattgtta ttatgtgggc tgatggtaaa agtgccgttc
    13381 ggatgaagtg aaattttaaa aagcagttcg taagcttccg gagccacata atcggtttca
    13441 ataataatag gagttgtagt ataaagatta cgggttttga aacgaccaat cgtcaaatag
    13501 tccactttca taaaaggtaa actatctaca actttgccaa tcaaatcgta tgaattgaga
    13561 attatcacct gattattgac attctggtaa gcttcttcca acccaaatcc ctgcatgaaa
    13621 ccttgtgaag aggatcgata tttgttttca ttaataataa ttgttccaac actctgatat
    13681 agaggcatcc aacttctgtt ttttaaatat cctccaatta aaaaaaacgg aataaatatt
    13741 aaaaacaaat accaattttt gataaataaa tgaagccact tctgaaaatc aaaggaagat
    13801 tcttcttcgg taaaattctg gttaatgctg cctgtatccg gattcatatc ttagaataaa
    13861 agataggttt atgctgtgaa tttacaattt gatataatat aaaacgctga acaataaagt
    13921 taaagaagac gaaatcagtc caagaaaaga agaataattg ttcattttaa aaaagctcga
    13981 cgatgcccgc tgcacataaa taatatcgtt ggggtaaaca taatagtatt ttgattctat
    14041 tacacttgcc ggacgaatat ca
//
LOCUS       DAOSCH010000051        13963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_2961_length_13963_cov_6.187949, whole genome shotgun sequence.
ACCESSION   DAOSCH010000051 DAOSCH010000000
VERSION     DAOSCH010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13963
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2961_length_13963_cov_6.187949"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(163..4851)
                     /locus_tag="PLZ54_05335"
     CDS             complement(163..4851)
                     /locus_tag="PLZ54_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ66384.1"
                     /translation="MRRLSFYFQSMFIVLVTALQAQQIPPTNNTAFRYLKFQVDKAGT
                     STINYLEVGEVEWMVGTTAYPITKFTTNTTLVTSSATGEWGSSGINMYDGKINQAWVY
                     LRERPFPIWATLDNTTPIVPTSIKITIRPDCWFAGFSCYGSNDNINWYLLLDKQNYDK
                     ASTFGSGLTEKTVEFPIPAQLIDNQPPAVPTNIVASNVTSRSFDLTWSPSVDNEGGVG
                     FVTYEIYVDGKLRGTTQNTSYSVTGLSGNTFIVTIKAKDGAMPNNVSEASAPLIVQKY
                     IDPVLNNEPSMGQIMSKLYANRGQQIITLPQISDGEIDKKQTVTISVVSTTPELLTID
                     SVSYKQGNKVAFVYVKDQGKIGKASIEITITDDGGTAGGGKDKAVFNEQIDIQAFKNP
                     CANFVEYDTQHWQPRPSQNDFPTPGSYQKICTTNSPIEDKARDFFWMKMYGYIIPSKT
                     EYYNFQGFSNEGFDMFMNLNGGPESDWSKLTSLVEGATSIWTSEAYLLEEGKPYYFEV
                     YGRDIVNTQPLWLKWTSASTPLSFISNENLAPFYDIVKPTVPQNFSIQSIGLSDVLVS
                     WSPSTDNRNNLQGYNVFIDGELVNISPVKETSYKIQGLTPGRTYNVHVVSVDDSENNS
                     YPSQVLTFKTYIADDIPPTVPTNLSSEFVTAFSVKLKWNKSTDAETEVRGYNVYQNNV
                     LVNELVKDTTVLVIGLTEKSNYSFTVEAVDANYNKSPKSAAYNVSTVAFDPNETRDGI
                     RKGRVSIKLNPLTKFEGFGINLNYRKTGLLSANLITFGDFEGSQTDTMNVNNLKYYDK
                     QVFNTGFVKDLTTPYEGKVAAKLSPIAGGYFRCVINTSIDKRYTYLVRFAMKKDASFT
                     GVVNVDLKGMFSGTTNKVAVTPTTSWQMYEVELTTTFEGAEPTWYLDFTSTSNGTYYL
                     DNIQFVEKGSYVEGSKFSKRAMDLLRDFKPAHVRWGAIEANSENFKFTSGIGTGANFS
                     YADWIELANELNAKAFITVGVGPKTDFYLSPAATFKKFVEYIAGDATTAGGILRTAEG
                     YGNLLSKNKGLIIELGNEVWGGAAHQAGNFNDYTAYGTWARSAATTMKSTAGFDPNKI
                     KVAYSGRYPGQNYGLHKILLSEDKGEGDVLAISGYMGGNLNLDPTIPLKSSLLDYHKA
                     SIKMLQTNLEGLKSEWREMLATCNRLLPMYMYEGNMTTDSYNGSLGQAVTFADYYTTV
                     PLYGVPDVSVFSFESGQWRIIEDRISLKPLPLFTIGKYINKYCTGSMLETEFTTSYKI
                     KDEKGIDLTIEPVGARAYTDSTSYSIALFSRDFENDYQVQVDIPDFIGNSSICKMIRI
                     SGTNFDDREAIVEERIISDFADSLIITVPKYSVVILHFEGNDQHFTPPAVYQSYKKVT
                     SVKITQAENKYYIDTKRGKLALSALVLPEDAFSKIVKWTLLDNETVKAYFVESGSGIM
                     LYGSGVSSGNGTIRVVCAATDLSGKADTVSIVISNQGPSALDSFEAEIVEMHPNPAKD
                     YLRVTMPQSQAGQFFVLDLSGKIHIRQTITGNEQVLNIKSLKPGMYLLRISTLNGTKT
                     LKFIKQESPT"
     gene            complement(4983..7802)
                     /locus_tag="PLZ54_05340"
     CDS             complement(4983..7802)
                     /locus_tag="PLZ54_05340"
                     /inference="COORDINATES: protein motif:HMM:NF036578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HOJ66385.1"
                     /translation="MQTRILILLLFLAQLNTLFPMSKIQTVEPSYVWIDQTGEGRNVY
                     AYFRNEFQLIEVPQNAIIYLFAKSLYNLFVNGTFVNFGPIRSYETQPYYDSINIAPYL
                     KTGQNVIAVKVHSIGIHTYQIPMGTAGFIAWGNIQDKSNTVSLATPGNWICRKALGYN
                     PFSPRFSFAQGPVEILDNLAEPDNFKAEKIKPDKWMKPVLLKNQSYFGKLRPRTIPLL
                     TNDAILPKFVVNRLNLSSDECVYNISSYYENTENPSDLRAILCYSYIYSNEEKDVNFG
                     FSWGKYWINGQLIEGVKQPEKPFRSNAKVRLKKGWNSYFSVQEIIWSNLDGMLAVPPD
                     ADLQFSIRRKLNDTIRFALTEPIEKEKINTFVPNDKCPENPEKLKLKWLHTSGTAPML
                     NPAKAIAWLNKNKLPIAVLETENIIIPANTHQAVTYDFGSIHLGRIFLNVEAPAGTVF
                     DLVWSEDLNKDSLVTLYNRFEINAIARFISDGSSYFETFRPFGMRYLQVGVSNHSQPV
                     ILKSAGLKSQIYDFEKTGSFSCSDPLMNDIWEMGWRTLRVCAEDTYIDTPFRERGHYV
                     GDLFPEFATTLAVSGDPRLAKQSIRMFNQQYSKTYSDLAPTIHSDYPILNIIIACWYI
                     RQFNDLDFAREVYPIYANYLRKLHKSRQKDDFYRLDHVFLEWINIDKSADLTAIQSYV
                     YAASDNLSHIANMLGQLADASEFKSISNEIKEIILTKSWDEKSGNFVDGFKNNKVLPT
                     RHVISSSLASVWKIPSMRQEEQIQKWFNDAVINIGEPVNSNQLTTPYGGFYVLASLYQ
                     AGNVCTAEKFIRKHWGKMVIEGNDLTWEDFNRDGKSTISHAWSSSPTYYMSSQVLGVD
                     LGFPGYLSPDTIYIRPQSESVLWAKGSVPHPKGLVTVHWQIQGDILFLRYSAPAGVPV
                     KIEPRGRLASYKLKLTCLTGTGDKL"
     gene            complement(8076..8681)
                     /locus_tag="PLZ54_05345"
     CDS             complement(8076..8681)
                     /locus_tag="PLZ54_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66386.1"
                     /translation="MHKKVFLFLVMLINAFALYPKTIYIPSSGDNSNGSSSNLIFDAF
                     GVQNLPKPVVSGSNVNTGWSTYKNNILISGNISKSSNLFVNGKLMLVARWPNSGWINI
                     DSITELNGSLTLTCSKLAAHSAVAYNYWKVATMRWRRWSWWFETPTITASFSPDKLKI
                     PGSVAEDSADGWKFYIDNKFEELYTIGEWIYSKGKFMLYRR"
     gene            9818..11632
                     /locus_tag="PLZ54_05350"
     CDS             9818..11632
                     /locus_tag="PLZ54_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ66387.1"
                     /translation="MKTNWHHLIFVALLCIAASVFSVKAQGKYTATDFHQHTTYSDGS
                     YTFGYMMEKNNQFGLDWWVNSEHGGGFDRWGFASGADLGTEVSWSEAGIEKLGKANGE
                     KMWRWQSLRDWSFRDIMLYRKVYPNKTIIQGFEWNAPGHEHVNVCIINNQFDENTENC
                     LPIAEFEYKFDNNDSDNSNPNGWTKSTNTGKAKTLEAIQWLQTNYPNTSWVIPTHPER
                     ANTFKIEDFRNMNNAGPSVCFGFDSQPGHQKEPNRGGYRTTSYGANGEGATWGGTGYF
                     AAKVGGVWDALLSEERKWWLFANSDCHFNDGDFFPGEYQKTKVFVSNPKNPQSLVDGL
                     RSGNSYVVMGDLIDSLSFTVGSAKMGETFVTADNNVVVSIIVHDPQVDNIHNTYSALK
                     NPELDHIDLIAGKVTSLIDPQSPDYKVDSVTTTSVIARFGKSVHTDANGITTIPWEDL
                     GNGYFKITYTVNLNNQNMYFRLRGTHHAFGDANAEIDDKGNPLVDPFGQNTAEKAFND
                     LWFYSNPIFVTKSNQAGISSPKLENIHIYPNPAKDMLLIQFTEPQTGLIKFVDMSGKC
                     VLQSEIKNSVQEQISCSSLPKGLYFVKINNLMQKVILE"
     gene            complement(12583..13248)
                     /gene="lipB"
                     /locus_tag="PLZ54_05355"
     CDS             complement(12583..13248)
                     /gene="lipB"
                     /locus_tag="PLZ54_05355"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOJ66388.1"
                     /translation="MTRQIEFLDWGLTEYADAWKRQEDLFQLLLQNKKNADFNTHYVV
                     FCQHFPVYTLGKNGHEENLLLNQIQLQTRGASFFRTNRGGDITFHGPGQVVGYFIFDL
                     EKLQLGLKVFIYKLEEVIIHLLEHYGLKGTRLPGATGVWLDAETPRARKICAIGLRSS
                     HFVTMHGFALNVNTDLNYFNYIHPCGFVDKGVTSLQKELFQTVEEEEVKNLLKKQIEK
                     EFL"
     gene            complement(13251..13949)
                     /gene="mtgA"
                     /locus_tag="PLZ54_05360"
     CDS             complement(13251..13949)
                     /gene="mtgA"
                     /locus_tag="PLZ54_05360"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOJ66389.1"
                     /translation="MKNFIVLFFLISIGWVIIYRFVPVMVTPLMLIRTAESVFSGETP
                     KISKKWVPIDKISHNMIQAAVAAEDDQFLHHHGFSFEAINQAIEHNKRSRRIRGGSTI
                     SQQTAKNVFLWPQRSYVRKGLEAYFTVLIELFWSKERIMEVYLNVIEMGDGIYGVEAA
                     SMEYFGKHASKLTKSEAALIAACLPNPRRFNPAHPSGYVLRRQGRIMAMMNKIEPVHF
                     ERPKFKFLKHKKQK"
BASE COUNT         4249 a         2693 c         2573 g         4448 t
ORIGIN      
        1 agaattcccc gatgcttgcg tcggggtagc ttttgttttt atttttttgg aaatttcagt
       61 ggcgaagcca taaaattggc ttaatacctc gccgcaagca gcgaggtatt aagccaattt
      121 tatagaattt ctttatttct cttttttgaa gaaaatcagg ttttaagtgg gggattcttg
      181 ctttataaat ttcaatgttt ttgttccatt aagtgttgaa atgcgaagca aatacatacc
      241 aggttttaga cttttgatat tcaacacttg ctcattgccg gttatggtct gtcggatatg
      301 tatttttcca ctcaaatcta gaacaaaaaa ctgtccagcc tgactttggg gcatggtcac
      361 ccgtaaataa tctttggccg gattaggatg catttccaca atttcagctt cgaaactgtc
      421 gagagctgaa ggtccctgat tggaaataac aatactgaca gtgtcggctt ttcctgataa
      481 atcggtagcc gcacatacca ctctaatagt tccgtttccg gagcttacgc ctgaaccata
      541 aagcattata ccacttccac tttcaacgaa ataggcttta acagtttcat tatcaagtaa
      601 tgtccatttt actatcttgc taaatgcatc ctcaggtaaa accaaagcac taagtgccaa
      661 ttttcccctt ttagtatcaa tataatactt gttttcagct tgtgtaattt ttacagatgt
      721 tacctttttg tagctctgat atacagcagg tggggtaaag tgctgatcgt taccttcaaa
      781 atggagaata acaacactgt atttaggcac agtaataata agactgtcgg caaagtctga
      841 aattattcgt tcctcaacta ttgcttctct gtcatcaaag ttagttcccg aaattcgtat
      901 cattttgcaa atggaagaat ttccaataaa gtctggaata tccacctgaa cctgataatc
      961 attttcaaaa tcgcgactga aaagtgcaat cgaataactt gtactatcgg tataagccct
     1021 tgcacctacc ggttcgatgg tcagatcaat ccccttttca tctttaattt tataggaagt
     1081 agtgaattca gtttcgagca tggaaccggt acagtactta ttaatatact ttccgatggt
     1141 aaataatgga agaggtttca gagaaatacg gtcttcgata attcgccatt gtccactctc
     1201 gaaggaaaat actgacacat cgggcacacc atacaatggt accgtagtat aatagtcggc
     1261 aaatgtaaca gcttgaccca acgacccgtt gtatgaatca gtagtcatat ttccctcata
     1321 catgtacatt ggaagtagtc tattgcaggt agccagcatt tcgcgccatt cacttttaag
     1381 accttccaga ttggtttgta acattttaat ggaagccttg tggtagtcta acagggatga
     1441 tttaagcgga atagtaggat ccagattaag atttcctccc atataaccac taattgcaag
     1501 tacgtctccc tcacctttat cttcggaaag cagaatttta tgcaatccgt aattttgacc
     1561 cggatagcgg ccactgtatg ccacttttat tttattgggg tcaaaaccgg ctgtcgactt
     1621 catggtagtt gctgctgagc gtgcccaggt tccgtaagca gtataatcgt tgaaattacc
     1681 tgcctgatga gcagctccac cccaaacttc gttacctagt tcaattatca atcctttgtt
     1741 ttttgaaagt aagttgccgt aaccttctgc agtccgtaat atacctccgg cagtagttgc
     1801 atctccggct atatattcca caaatttttt gaacgtggca gcaggcgaca ggtaaaaatc
     1861 agttttcggt ccaacaccaa ctgttataaa tgctttagca ttaagctcgt tggctaattc
     1921 aatccagtcg gcatacgaaa aattagcacc ggtaccaatc cccgaagtaa atttgaaatt
     1981 ttccgaatta gcttctatag ctccccagcg aacatgggca ggtttaaagt cacgaagcaa
     2041 atccatagcc cgtttcgaaa atttactacc ttcaacatac gatccctttt cgacaaactg
     2101 aatattatca agataatacg ttccattaga agtggatgta aaatctaggt accaggtagg
     2161 ttcagctcct tcgaatgtag tggtgagttc gacttcatac atctgccaac tcgtagtcgg
     2221 agtgaccgcc actttattgg tagtacctga aaacattcct ttgaggtcga catttacaac
     2281 tcctgtaaat gaggcgtcct tcttcattgc gaaacgaacc agataagtat atcgtttgtc
     2341 gatacttgta ttgattacac aacggaaata tccacctgca atggggctca gtttggcagc
     2401 gacttttccc tcgtaaggtg tagttaaatc ctttacaaaa ccagtattga atacttgttt
     2461 atcatagtac ttcagattgt ttacattcat tgtgtcggtt tgtgaacctt caaaatcgcc
     2521 aaaagtaatt aaattggcac taagcaaacc agtttttcgg taattcagat ttatgccaaa
     2581 accttcgaac ttagtgagag gattaagttt tatactcact cgtccttttc ggataccatc
     2641 acgtgtttca ttgggatcaa aagccactgt tgacacatta tatgcagcac ttttaggtga
     2701 tttattataa tttgcatcca cagcttccac tgtaaaactg taattgcttt tttcagttaa
     2761 tcctatcact aacacagtag tatctttaac taattcatta acaaggacat tattttgata
     2821 aacattgtag ccgcgcactt cggtttcggc atcagtagat ttattccatt ttagcttcac
     2881 cgaaaatgct gtaacaaatt cgctgctgag atttgtagga acagtaggtg ggatatcatc
     2941 agcgatgtag gttttaaaag tcaaaacttg ggaaggatac gaattatttt cagaatcatc
     3001 taccgaaacc acatgcacat tgtatgtcct gccgggtgta agtccctgta ttttgtacga
     3061 tgtttctttt acgggactaa tgttaaccaa ctctccatca ataaacacat tatatccctg
     3121 aaggttgttg cggttatcgg ttgaagggct ccaactaaca agtacatctg ataaaccgat
     3181 gctttgaata ctaaaatttt gcggaacagt tggttttaca atatcgtaaa acggtgccag
     3241 attttcgtta gaaatgaaac tgaggggcgt gctggcactg gtccacttta accaaagtgg
     3301 ctgagtgttt acaatatcgc gcccatatac ttcgaaataa tacggtttac cttcttcgag
     3361 caaataagct tctgaagtcc atatgctggt agctccttct accagcgaag ttagttttga
     3421 ccaatcactt tcaggcccgc cgttcaggtt cataaacata tcaaaaccct cgttggaaaa
     3481 tccctgaaaa ttatagtact ctgtttttga tggaataata taaccataca ttttcatcca
     3541 aaaaaagtca cgagccttgt cttctatcgg agagttagtt gtacaaattt tctgatacga
     3601 acctggtgtg ggaaaatcgt tttgcgaagg gcgtggttgc cagtgttgag tatcatattc
     3661 cacaaagttt gcgcaaggat ttttgaatgc ctgaatatca atctgctcat taaacacagc
     3721 tttatctttt cctcctccgg cagtgccacc atcgtcagta atagtaatct ctatactcgc
     3781 tttgcctatt tttccctgat ccttaacata tacaaatgcc actttattac cttgtttgta
     3841 actcactgag tcgatggtta gtaattcagg cgttgttgaa acaactgaaa ttgtaacagt
     3901 ttgttttttg tctatttcac catcgctaat ttgtggtaaa gtgataattt gctgaccacg
     3961 attagcatac aattttgaca taatctgacc catacttggt tcgttattaa gaacagggtc
     4021 aatatatttt tgtacaatta acggtgcgct tgcctccgaa acattatttg gcattgcacc
     4081 atcttttgcc ttaattgtta caataaaagt gttgcctgag agtccggtta cagagtaact
     4141 agtattttgg gtagttcctc ttagttttcc atccacgtaa atttcataag ttacaaaacc
     4201 aactccgcct tcattatcaa ccgatggaga ccatgtcagg tcaaaggagc gggaagttac
     4261 attcgatgca actatatttg ttggtactgc cggaggttga ttatctatta actgtgcagg
     4321 aatcggaaac tctacagttt tctctgttaa accactgcca aaagttgaag ctttatcgta
     4381 attttgtttg tcaaggagta aataccagtt aatattgtca tttgagccat agcaggaaaa
     4441 tcccgcgaac cagcaatcgg gacggatggt tatttttata cttgttggta ctatcggagt
     4501 agtattatca agggttgccc atattggaaa tggtctttcc cgaagatata cccatgcctg
     4561 attaattttt ccatcgtaca tatttatacc tgatgagccc cattcccccg tagccgaaga
     4621 agttaccagc gtagtgttgg ttgtgaattt tgttatcgga tatgccgtgg tgccaaccat
     4681 ccattccact tcaccaactt ctaaataatt aattgtcgat gtacctgcct tatctacctg
     4741 aaatttcaga tacctgaatg ctgtattatt tgttggtgga atttgctggg cttgtaatgc
     4801 agttacaagc acgataaaca ttgactgaaa ataaaagctt aatcttctca tactattaat
     4861 tctttagtta gttgaagcaa agataatttt acatcctcaa aaaaaaacaa tactttttta
     4921 tccataaatg atagttagtt tatatcacta tcagaacatg gatataagtt ttacattcaa
     4981 acctataatt tatcccctgt gcctgttaag caagtaagtt ttaatttata ggaagctaat
     5041 ctgcctcgtg gttcaatttt taccgggaca ccggcaggag cactgtatct aagaaaaagt
     5101 atatcgccct gaatctgcca atgcacagtt accaatcctt ttgggtgtgg taccgatccc
     5161 ttagcccaca atacggattc actttgtgga cgtatgtata tggtatcggg tgataaataa
     5221 cccggaaatc ccagatcgac acctaatacc tgcgagctca tgtaataagt tggtgagctt
     5281 gaccatgcgt ggctaattgt tgatttcccg tcgcgattga agtcttccca agttaaatcg
     5341 ttgccttcta tcaccatttt accccagtgt ttgcgaataa atttttcggc tgtacaaaca
     5401 ttgccagcct gataaagaga tgctaacaca taaaaaccgc cataaggtgt ggttagctga
     5461 ttcgagttta ccggttctcc gatattgata accgcgtcat taaaccactt ttgaatttgc
     5521 tcttcttgcc tcatggaagg tattttccat actgatgcca aagaacttga gatgacatgt
     5581 cttgtaggaa gcaccttatt gtttttaaaa ccatccacaa aattgcccga cttttcgtcc
     5641 cagcttttag tcagtataat ttcttttatt tcattcgaaa tagatttgaa ttcagaagca
     5701 tcggcaagct gtccaagcat atttgctata tgagataagt tgtcggaggc agcataaaca
     5761 taggattgta tagccgttaa gtcagccgat ttgtcaatgt ttatccattc cagaaaaaca
     5821 tgatccagac gatagaaatc atctttttgc ctgctcttat gcaattttct caggtagttg
     5881 gcgtaaatgg gataaacttc acgggcaaaa tccaaatcgt tgaactgacg aatataccaa
     5941 caagcaatga taatattcag aatcggatag tcagagtgaa tcgtaggtgc caaatccgaa
     6001 taagttttac tgtattgctg attaaacatg cgtatagatt gttttgctaa tcgaggatcg
     6061 ccactcactg ctaaagttgt tgcgaattcg gggaacaaat cgcctacata atgaccacgt
     6121 tcgcgaaatg gtgtatcgat atacgtatct tctgcacaaa cacgcagggt acgccatccc
     6181 atttcccata tatcattcat tagaggatcg gaacagctaa aacttcctgt cttttcaaag
     6241 tcgtaaatct gacttttcag gcctgcactt tttagaatta caggctgtga atgattgctt
     6301 acacctactt gtaaatacct catcccgaaa ggacggaaag tttcgaaata cgaacttcca
     6361 tctgagataa aacgggcaat tgcattaatt tcgaatcggt tatataaagt tacaagagag
     6421 tctttattta aatcttcgct ccaaactaaa tcgaaaactg tacctgcggg agcctccaca
     6481 ttgaggaata ttcgccccag atgaattgac ccaaaatcgt aagtaacagc ttgatgtgtg
     6541 ttggcaggaa taataatatt ttcagtttcg agtacagcta tgggcaattt attcttgttc
     6601 aaccaggcta tcgctttggc aggattcaac ataggagctg taccagatgt atgcagccat
     6661 tttaatttca gcttttcggg attttcgggg cacttatcgt tgggaacaaa agtattaatt
     6721 ttttcctttt cgatgggttc tgtcaatgca aaacgtatag tatcgttaag tttgcgtcga
     6781 atcgaaaatt gtaaatctgc atcgggaggg actgcaagca taccatccag attcgaccag
     6841 ataatttcct gtactgaaaa gtaactgttc caaccttttt ttaaacgcac ttttgcatta
     6901 cttctgaacg gtttttcagg ctgttttaca ccttcgataa gctgtccatt tatccaatac
     6961 ttaccccaac tgaatccaaa gttcacatct ttttcttcgt tagaataaat ataagaataa
     7021 cataaaattg cacgcaaatc ggaagggttt tcggtgtttt cgtaatacga agatatatta
     7081 tagacacatt cgtcggagct taggttaagt ctgttcacca caaatttcgg caaaattgca
     7141 tcattagtta ataacggaat tgtacgggga cgtaattttc caaaatagct ttgattcttt
     7201 aataatacgg gtttcatcca tttatcaggt tttatttttt cagctttaaa attatcaggt
     7261 tcagccaagt tatctaaaat ttcaaccggt ccctgtgcaa aggaaaaacg aggtgaaaaa
     7321 gggttgtaac ctaatgcttt cctacaaatc caattacctg gtgttgccag cgaaactgta
     7381 ttgcttttat cctgaatatt gccccaggca atgaaacctg ctgtacccat aggaatctga
     7441 taggtatgaa tgccgatact atgaactttt acagcaatta cattttgtcc tgtttttaaa
     7501 tatggcgcaa tatttatgct atcgtagtat ggctgtgttt cgtaactacg aattggcccg
     7561 aaattaacaa aagtgccatt cacaaaaaga ttgtaaagtg attttgcaaa cagataaatg
     7621 attgcatttt gtggcacttc tatcaattgg aattcattcc ggaaataggc atatacattt
     7681 cggccttcac cagtttgatc aatccataca taagaaggtt caactgtttg aattttgctc
     7741 atgggaaaca acgtattaag ttgagccaaa aaaagaagta atattaaaat tctggtttgc
     7801 attctgattt ctattttttt aattctttaa tatgctttat ttaaaataca ttttgttcct
     7861 taataagtct caaaagagtt tcatttttat caatgtcaat tatccgtatt acaattatat
     7921 attatcgaaa ctgcaatttt cgataacaca ggcttcattg atactcagct cgtggtcggt
     7981 ttggtaccgg aaaaccacat ttcaaatggt taatgcattt agttacaaac cataactgag
     8041 cactgcacct tcgataaggt attgatttgt tgcgcttacc ggcggtataa cataaacttt
     8101 cccttcgaat aaatccattc gccgattgta tataattctt cgaatttatt atcgatataa
     8161 aatttccaac catcggctga atcttcagca acagaccctg gaatttttag cttgtcggga
     8221 gaaaaggatg ctgtaattgt tggtgtttcg aaccaccagc tccatctgcg ccagcgcata
     8281 gtggctactt tccagtaatt ataggctact gcactgtgag ctgcaagttt tgaacaagtg
     8341 agcgtaaggc ttccgtttag ctcagtgatg ctgtcaatat ttatccatcc actattgggc
     8401 caacgggcaa ccaacattaa ctttccgttc acaaacaaat tggaagactt tgatatattg
     8461 cccgatatca ggatattatt cttgtaagta ctccagcctg tattgacatt acttccactc
     8521 acaacaggtt ttggtagatt ctgaacgcca aaggcatcga atataagatt cgatgatgaa
     8581 ccattgctat tgtcgcctga tgaaggtatg taaatggttt ttggataaag cgcgaaagca
     8641 tttatcaaca ttaccaaaaa taaaaatact tttttgtgca ttcgatttaa aatagattat
     8701 tctttttttt accacacaga atactgtaat ttgcatttta tatcagcttt agataaaaat
     8761 gagacagaaa gaacagcaaa gcaaatggtt gatttcttat tcatcgttgt gctcgtaata
     8821 aaattaattt cggtaagtac aaaaatacgt gaaaaagtag agttttaata atcttttttt
     8881 gaccaatatt gatatttaag ttccatttac ctataaaact cattgaaata tataaattta
     8941 tgtatgaaaa gtattaacct attcgtaaca atgtgcttaa ttttgtttac tgcatcaaaa
     9001 ttttttattt ttatttcatc ccaaggatgt ttttttagat taacttcatg attataagtt
     9061 ggttgctgaa tgaaatgtat atttccgggc actcttaaac cttttgcttt gttccatcct
     9121 gctccatcta tctgcatgat aatttcaaat tccttaaatt cattggatac aggatacatt
     9181 ttctaaaaat aaactcatca tttcggtatt tgcatacagt aatattaaag aactaatgtg
     9241 gcctaacttc ggacatattc ctgcataaac ataaaaactt tctcttacca cttgttttgc
     9301 taccagagga cgaacctgtt tggttgccca acaacttctt ggaatattta ctcttccaaa
     9361 tcatcttttt ggtttgaatt taagatacaa attgttgact tgataaacgc tatgttttac
     9421 caagtcaaca ataatttaat acaaaaatgg ttttttaaag ctcgactaat taacaattat
     9481 agattacaaa ttcattgatt aacaaagctt tgcattagaa atcctcgctt cgatggaagc
     9541 agaagccaca catgcaacag ttacacgtca ttacggactt caatagcaag gaaaagaaac
     9601 ttctaccaat cctattactt ccatttttgc ttggacgcgc cgactggtaa aaagcagaaa
     9661 actaaaaaga taaatggaaa aaattaactc tttccgaaac attctgttca tatcaatttt
     9721 caaacaatga aatattcgcg taaccaattg ataacattac cctcttactt ttgccataga
     9781 attaaaaaca aattactaac taaaaaatta catttaaatg aaaacaaatt ggcatcattt
     9841 aattttcgtt gcattgttgt gcatagcagc aagtgtattt tcagtgaaag cacaaggaaa
     9901 atatactgca acagatttcc atcaacatac tacttattct gatggaagtt atacgtttgg
     9961 ctacatgatg gaaaagaaca atcagtttgg attggattgg tgggtgaata gtgaacacgg
    10021 aggtggattt gacagatggg gatttgcatc cggagccgat ttaggtacgg aagtaagctg
    10081 gtcggaagca ggtatcgaaa aattggggaa agcaaacgga gaaaagatgt ggagatggca
    10141 atcgttgcgc gactggtcgt tcagagatat aatgctttac agaaaagtat atccaaacaa
    10201 aacgattatt cagggttttg aatggaatgc acccggacat gaacatgtaa atgtttgtat
    10261 tatcaacaac cagtttgatg aaaacacaga gaattgtctt cctattgccg aatttgaata
    10321 caaatttgac aacaatgatt cagataattc aaatcccaat ggttggacaa aaagtacaaa
    10381 tacaggaaaa gctaaaacat tggaagccat tcaatggttg caaacgaatt atccgaatac
    10441 ttcatgggtt atccctacac accccgaaag agccaatact ttcaaaattg aagattttcg
    10501 taatatgaac aatgctggtc cttccgtatg ttttggtttt gacagtcagc ccggccatca
    10561 gaaagaacct aatcgaggcg gttatagaac tacttcttat ggagctaatg gtgaaggagc
    10621 tacatgggga ggaaccggat actttgctgc caaagtagga ggtgtatggg atgctctgtt
    10681 gagtgaagaa cgcaaatggt ggttgtttgc caactctgat tgtcatttta atgatggtga
    10741 tttctttcca ggtgaatatc agaaaacaaa agtatttgta agcaatccta aaaatccaca
    10801 atcgctcgtt gatgggcttc gttcgggtaa ctcttatgtg gtaatgggag atttaatcga
    10861 ttcattaagt tttactgttg gttcggcaaa aatgggtgaa acttttgtta ctgcggataa
    10921 caatgtggtt gttagtatta ttgtgcatga tcctcaagtt gataacattc ataataccta
    10981 ttctgcttta aaaaatcctg aactggatca catagacttg atagctggga aagtaacttc
    11041 acttattgac ccacaaagtc ctgattacaa agtcgatagt gtaacaacaa cttctgtaat
    11101 cgcacgattt ggcaaatcag ttcatactga tgccaatggt atcacgacca ttccgtggga
    11161 agatcttgga aatggatatt ttaaaattac ttacacggta aatttgaata atcaaaacat
    11221 gtatttccgt ttgcgtggaa cgcatcacgc tttcggagat gccaatgccg agatagacga
    11281 caagggaaat ccactggttg atccgtttgg ccaaaatact gccgaaaaag cttttaacga
    11341 cctctggttt tacagcaatc ctatttttgt aacgaaaagt aatcaagccg gaatttcttc
    11401 tcctaaatta gaaaatatcc acatttatcc caacccagca aaagatatgc ttttaattca
    11461 gtttactgaa cctcaaacag gcctcataaa gtttgtagat atgtcaggta agtgtgttct
    11521 gcaatcagaa atcaaaaatt cggttcaaga acaaatttct tgcagttctt tgccaaaggg
    11581 attgtatttt gtaaaaatca acaacttaat gcagaaagta atattggaat gattaattga
    11641 tttatatgaa accctcatgt attgcataca caaatacgaa tgaatataat attcagacat
    11701 gtgggttcca tcagaccttt aaaaaataaa ttatattctg atgaaagatt aaattgttaa
    11761 aatttttaat aatcttatta tcaaatatat ataacactta cgatcaaaag agggtgtccc
    11821 aaaagggcac tttctttttt aaagtaagca gccgatttaa gggttaaatt ttgatagaaa
    11881 atagctttat ttttaatttt attttggaac aagcaaaaaa taagaataaa ggtgttttta
    11941 gcattttttg tcgatttgtt gattttcagt tccagtttga acattgtgaa caggtttgag
    12001 ttgtccgttt ttcatccgat atcaacatga atgacattca aacttaaata gttcatatct
    12061 aatagcataa gtacaacttg ggtaaatatc cagtgaatat cctcctttaa atgacaggaa
    12121 cgaaaacggt tgatcgcatg aaagccacat gaaactgatc cggtctgcaa gtgcctgctc
    12181 tatttttcga atcgaataaa tattgttcaa ataactgtac aaaaatactt cagcatcatt
    12241 agcggttgat aggcacttgt tcatcctccc ttttaagtat caatgatttg agcaatatcc
    12301 aaattatcta ctatttgatt aaccaactgt gcaggagaat cttgtggtac tttttcatcc
    12361 aaactggcag ggaacagaac gatttgtcct tgttcattgg atttaaaaac tactctggac
    12421 gtaattcatg ttattgatta acaaataaaa agatacaaaa aaaaatattt ctacaaattt
    12481 attcttccta tttttaatcc aatatccatt aaaaaagatt gcaacaattc ttgaacagct
    12541 ccatcgggaa atggaaaaaa caaccacaaa tttttaggtg tattacaaaa attctttttc
    12601 gatttgtttt ttgagtaggt tttttacttc ttcttcctct acggtttgga acaactcttt
    12661 ttggagggaa gttacgccct tgtccacaaa gccgcaggga tggatgtaat tgaaataatt
    12721 caggtcggta ttgacattga gcgcaaagcc gtgcatggta acaaaatgcg agctccgcaa
    12781 gccaatggcg caaatttttc ttgcccgggg cgtttcggca tccagccaca cccctgttgc
    12841 tccgggcagt cgtgtaccct ttaacccata atgttcgagt agatggatga tgacttcttc
    12901 gagtttgtaa ataaaaactt tcagtcccag ttgcaatttt tcaaggtcaa aaataaaata
    12961 accaaccacc tgccccggcc catgaaaagt gatatcgcca cccctgttgg tgcggaaaaa
    13021 acttgcgccg cgggtttgca actgaatctg gttgagcagt agattttctt catgtccgtt
    13081 tttccccaac gtataaacgg gaaaatgctg gcaaaaaaca acatagtgcg tgttaaaatc
    13141 ggcatttttt ttgttttgaa gcagcagctg aaacaaatct tcctgccttt tccacgcatc
    13201 ggcatattct gtcaatcccc aatccaaaaa ttcgatttgc cgggtcatgg ttacttttgt
    13261 tttttatgtt ttaaaaattt aaatttcgga cgttcaaaat gcaccggttc gattttgttc
    13321 atcatggcca tgatgcgacc ttgccgtctc aatacatatc ctgaaggatg ggcagggttg
    13381 aaacgtcggg gattgggcag acaggcagca atgagtgccg cttcggattt tgtgagtttg
    13441 gaagcatgtt tgccgaaata ttccatcgaa gcggcctcca cgccgtaaat tccatcgccc
    13501 atttcgatca cattcaggta aacttccata attctctcct tgctccagaa aagttcgatc
    13561 aatacggtaa aataagcttc caatcctttt ctgacgtaag aacgctgcgg ccagaggaaa
    13621 acattttttg ccgtttgctg cgaaatggta ctgcctccac gaatgcgacg acttcgttta
    13681 ttgtgttcaa tggcttgatt gatggcttca aaggaaaaac catgatgatg caaaaactgg
    13741 tcgtcttcgg cagccactgc tgcctgaatc atattgtgcg aaattttgtc gatgggcacc
    13801 cattttttgc tgattttcgg cgtttcgccg gagaaaacgg attcagcggt acgaatgagc
    13861 atcagcggcg taaccattac cgggacaaac cgatagataa tcacccatcc aatgctaatg
    13921 agaaagaata aaacgataaa gtttttaatg aatttaaaag ctt
//
LOCUS       DAOSCH010000052        13517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3085_length_13517_cov_5.018497, whole genome shotgun sequence.
ACCESSION   DAOSCH010000052 DAOSCH010000000
VERSION     DAOSCH010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13517
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3085_length_13517_cov_5.018497"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..3923
                     /locus_tag="PLZ54_05365"
     CDS             12..3923
                     /locus_tag="PLZ54_05365"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOJ66390.1"
                     /translation="MRKIFKHIIRTIGFLIITPVILLLLALGALQTPFVKSLLVDFAE
                     LQAEKVLNGQLRIGKLEGNFINGLRLKQILLTNHSDTLIWIPMLKIDYQLAPLVSGNI
                     SIHSLTINRLQVKLMQQKNGRWNFQELIKTPSQPTEKTSPFKMRITLENLQLNNAQIF
                     ILSENPSLPEKISDLHLKCNGEYSDKQSKLFMEEFRLTSHQPDLQIKELKFNFVQENE
                     ILYLSDFFLRTGNNTLTANTKIDISASETSFLSVKTAPLFLNEFADFFSAPLQENIHP
                     LVHVESIMQDDNLRLTLNLSEKKEKIEINLQVSKITEIIRQPKLTDNVTYTLMANVDQ
                     MELNKWINLSQPNILLNGEIFINGKGISLNTMEMGIKGDFGKLKINNQDLHKLNFNFD
                     YASKKLTGQLFAESPFGNLYLQSAIKEPTESIPSYKFLLVTRKLNPSTLFPAMKGSSD
                     LNMKIELEGSGFDPLQMTAYAKIQVDTSSISGWTIHSLQSNLNFQQQNFFIENFSVET
                     ASAHLSANGIYSIKGNSDAFLNLTLNNASEIIKFTGVENLNGRGKIAGHFHGAPKKFL
                     TDLNLTLFDTQFQTYSAEEVTAHITAQIPNDRNFSVSGNLNAKNLNLGGIELQSIQLV
                     SNADSKNIQLSSIAESGEFNTSFKSAIHLDNPLLVELSDFRINYRNNSWKQGTDTALF
                     YLSETDYRLEHLKLISEDADTLQTLSAEGKISKNGEQNFQISLSNLDFAKISETFFPE
                     QSLQGKINFNVQLMGEASSPVVEAKFRLNNFVLQNYYAGNMTGALQLSQQQLNTKVNI
                     NLPDSGKLSADAKIPVNFRLDSMQFNIPSYENENFDFNLFANKIPLTLLQSFLPDGQY
                     EGIIQTSMHAGGNIKEPELKGKIEITDGKIKMPQYGINYPHFMAGISMDNNKIKIDTF
                     LITSRDGRMNINGNMSPPTFLSKSTNAPSPLANVQIHFANFRPIDHKNYNMAVSGNAL
                     LKGNADSLLFSGDINIPQAEINLPSLLQLVGKTSAPQLPEPLLIKELKKMQPEDSLVI
                     TLRPRNEESKKEETDFLKKLEGNLRVKIPRNTWIKNQDMRVELSGDVQLMKHKEFFEL
                     FGSVEVLRGQYNLLGKVFIIESGTISFQGGEKINPLLNFTAAYNFRDSNRQQKKLLAN
                     ISGEMMSPEIKFTMDNQQIDEGDALSYILFGAKLDALTSGQRENINQTFDALGLTESL
                     AASGLSSQLTKLLSNTLNVDYIEVKSKGSFESGSLEVGKYLTNKIFINYVRNFGNYQR
                     DENFSEYEIRVEYEILKFLFLQLTTSPVKNGIDIVIKPTKK"
     gene            complement(4051..5418)
                     /locus_tag="PLZ54_05370"
     CDS             complement(4051..5418)
                     /locus_tag="PLZ54_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ66391.1"
                     /translation="MQEFTFARKKVKPHLSFFRILSLSMGFLGIQFGYALQNANASRI
                     LQGFGADIEQLSWFWLAAPLTGMIVQPIIGHYSDHTWTRLGRRRPFFLAGAFLAAIAL
                     VLMPNAGVFADVLPAMFVGAGFLMIMDASINVAMEPFRALVADMLPAEQTTVGFSVQS
                     FLIGIGAVIGSWLPYVLAEWLGIAKTAPEGQVPPNVIFSFYIGAVVLIVTILWTVFTT
                     KEYSPEELQAFESEEERTEKKNPKFSDIFKDMATMPKTMKQLSITQFFTWIALFGMWV
                     FTTPAIAQHVYGLPVTDTSSPTYQDAGNWVGILFGVYNGVAMLYALVLPAIARTIGRK
                     WTHSLSLMCGGVGLLSIFFINDPQWLILSMIGVGIAWGSILSMPYAILAPSLPPAKTG
                     VYMGIFNIFITVPQIVNGLFGGMVVKRIFDSQAIYALIMSGCFLLIAAISVLWVEDKA
                     EIRHR"
     gene            complement(5494..6504)
                     /locus_tag="PLZ54_05375"
     CDS             complement(5494..6504)
                     /locus_tag="PLZ54_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ66392.1"
                     /translation="MKNSQITIKDIARALNISPSTVSRALKNHPDISQETKDAVNKYA
                     REYNYRPNVMAMNLRMSKSNTIGVIVPEIIHYFFSSILSGIEQVANKEGFNVIICQSS
                     ENYEKEVRNVDALISARVSGILASLSKNTSTYGHFQEIVDHNIPLVFFDRICIGISTD
                     RVVVDDYAGAFAAVEYLIQTGCKKIAFYTSPLHLEISKNRRNGYLDALHKYGLPVDES
                     LIRVCDTREEAIIITPEILDRPDRPDGFFAINDHCASGILYAAKLAKLHVPQDISICG
                     FSDGELAKACDPMLTTVEQHGYEMGISAAELLISKIKGETQGKYTNKIIKTNLIIRGT
                     TR"
     gene            6938..10030
                     /locus_tag="PLZ54_05380"
     CDS             6938..10030
                     /locus_tag="PLZ54_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ66393.1"
                     /translation="MMNNHFKLKIFTFGMALLFSVAGNLSNAQQIAISGKVIDSNTGD
                     PIIGANILEKGTTNGTITNYDGEFTLNVASDAVIIVKYVGYKPLEIPVAGKRKFTIKL
                     EEDVITLGEVVAIGYGTVKKTDATGSVTAIKPDAMNKGQVTNAQDMLLGKIAGVNITT
                     GGGTPGTGATIRIRGGSSLNASNDPLIVIDGLPIDNNGIKGVANQLSTINPNDIESFT
                     VLKDASATAIYGSRASNGVILITTKKGSKSGKPKVSYDGNVSLSTLNKRIEVLNGDEF
                     RALVNQVYSGDGQTDIDARNAMGTENTDWQDQIYRNAFSQDHNISVVGGIKNTPYRVS
                     VGYTNQNGIIKTSYFQRYTGSINLSPSFFDDHLKFNLNGKGMFAKNRFADGGAVGAAL
                     TMDPTHPVKTDDPEFQRFGGYWQWFEKNTNGTFKSINTNATKNPMALLEQKRDVSKAN
                     DFIGNAEVDYKFFFLPELKAHLSLGIESSYGKQDLHIDSTATSDYPWGRTGWDKIEKT
                     NRLLNFYFQYAKEINKHSFDIMGGYEWQHYYREGQNEYRSLEKIDSNNDGIIDENDGY
                     YNHITPDQSLWKTENYLVSFFGRLNYSLAGKYLLTATLRDDGSSRFAPENRWALFPSV
                     ALAWKISEEHFISERGTFSDLKLRLGYGITGQQDISQGDYPYIPVYTISKEHALYPFG
                     NEYYYTSRPDAYNKLLKWEQTTTWNAGLDAGIFNNRLTGSLDYYFRKTNDLINVVSVP
                     AGTNFRNKVISNIGSLENKGIEFMISGKPISNKNCTWDISYNVTYNKNRITKLTTGNN
                     EGYFVATGGIFQGSTQAHTVGYPSNTFYVYQQIYDENGKPIEAGAPKPDGSGDVYKDI
                     EAFVDRDGNNTITEDDKYFYKSAHPDYTMGLSSKFIYKSFDIGFSMHASIGNYVYNAV
                     AAERLNVGDNGIYSLGYLSNKPKSALKTNFQGTSPITYLSDYFVENGSFLRCDNITIG
                     YSFNNLFKAISSLRIYAAVQNPFLITGYSGLDPEIFGGIDNNIYPRPMTTLLGLSVNF
                     "
     gene            10044..11669
                     /locus_tag="PLZ54_05385"
     CDS             10044..11669
                     /locus_tag="PLZ54_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOJ66394.1"
                     /translation="MNNKYLNIFGVLLVAFTFHACINDLDVTPINPQVTQTFNQDQVF
                     AKVYAAYALTGQEGPAGNNDIDIVDEGRFSLFRCLWNCNELSTDEAACAWGDAEVIDL
                     NQNTWTAGNFSTEALYARLYFIVSISNHFLEETAKITDEKTLKQRAEVKFLRALAYYY
                     LMDNFGNVPFTTIVGTKAPHQIERANLFAWIRGELKAIENDMYKPREAPYYRVDKAAN
                     WLLQARLYLNSEVYTGTAKWDSAAIYAKKVIDSGYQLAPTYRHLFMGDNAGTLDGSSV
                     NSAPQEIIFPLAADGIKTQSWGLSLFLIGSTYTSDMGDWGTNQGWAGNRARQDLVKKF
                     FPNPPSVIAKDTNMTMITFPSYVKDDRALFFRKSRTLSIVNLGKFKEGYSVIKFSNRR
                     ADGGTPHDPLFVDMDVPFMRLAEAYLTYAEAVLRGAPEIDGYSALAAVNALRTRAHSG
                     KIATQITLTDAENFTTGKFNIINEWAREFFFEGRRRTDLIRFGMFGGDTDYKWEWKGG
                     EQAGKNFSADFNLFPIPTSDLNANSNLIQNPGY"
     gene            11880..13118
                     /locus_tag="PLZ54_05390"
     CDS             11880..13118
                     /locus_tag="PLZ54_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66395.1"
                     /translation="MKKLFTFMGALLSLFAVVQMSAAADLVFNVTVPTPTYECWVVGN
                     FNGWNNNQYKMTKVDDTHYTITIPESEIVPGLTQIEYKYLSGGGDWAYVEKDADGNEI
                     SNRIYTDGNDVVQKWALVYNPNVEPIPMTVTIIAQVPADVIELYIVGTFNNWTIPTDT
                     TKMTFLEANESGKVFSATFFTVDANKLQFKFCAGPAWDYEQTQGANYTYPDPTQNTAA
                     VIVESFKKIYDPTKVGDVTIIATVPAGTERVWIMGSHLGWYWANLQEGTMNPDGTFTF
                     VVPDVMSFEYRLYNWYEPEFGYPEVGEADPTKDLPNRTAQYDPTNPANNTINITVWGW
                     KQPAPSAVSNVFFDNFQPKIVDRKIKVDNVTSSVELFDVMGRQLEKANVRGTYTTRTL
                     TNGIYILRVDGKFTAKIAVK"
BASE COUNT         4573 a         2621 c         2488 g         3835 t
ORIGIN      
        1 ttaaaaacct tatgcgaaaa attttcaaac atatcatccg tacaataggg tttttaatca
       61 taactccggt gatcctgttg ttacttgcgt tgggggcatt acaaacaccc tttgtaaaat
      121 cccttttggt tgattttgct gaacttcagg ccgaaaaggt attgaacgga caattacgca
      181 taggtaagtt ggagggaaat tttataaatg gtttacgtct aaaacaaatt ttgttgacca
      241 accattcgga cacgttgata tggataccaa tgctaaagat agattaccaa cttgcaccgt
      301 tagtttcagg taatatttcg atacattcct taacaattaa tcggttgcaa gtaaaactga
      361 tgcagcaaaa aaatgggcgt tggaattttc aggaattaat aaagacccct tcacaaccca
      421 ccgaaaagac atctcccttt aaaatgagaa taacacttga aaatttacaa ttgaacaatg
      481 ctcaaatttt tattctttca gaaaatccct ccctgcctga aaaaatttcc gatcttcatc
      541 tgaaatgcaa cggggaatat tccgacaagc aatcgaaact tttcatggaa gaatttagac
      601 taacctcaca tcaacctgat ttacaaatta aagaactgaa atttaatttc gtacaagaga
      661 atgagatatt atatctttcc gatttttttc ttcgtaccgg caacaatact ttgactgcca
      721 atacgaaaat cgacatttca gcttcagaaa cttccttttt aagtgttaaa actgctccct
      781 tgtttctaaa tgaatttgca gatttttttt ctgctccttt gcaggaaaat attcatccac
      841 tggtgcatgt cgaatcaata atgcaggatg ataatcttcg gctcacatta aacctgtccg
      901 aaaaaaagga aaaaatcgaa ataaatcttc aggtttcaaa aattacggaa ataattcggc
      961 aacccaaatt gaccgacaat gttacctata ctctgatggc caatgtagac caaatggaat
     1021 tgaacaagtg gatcaacctg tctcaaccca atattttgct caatggagaa atattcataa
     1081 acggaaaagg catttcattg aatacgatgg agatggggat aaagggtgat ttcggaaaat
     1141 taaaaattaa caaccaggat ctacataaac tgaatttcaa ttttgattat gcttcaaaaa
     1201 aattaacagg acaattattt gccgagagtc cttttggtaa tctgtattta cagtcagcaa
     1261 taaaagaacc taccgaaagc attccatctt ataaatttct gcttgtaacc cgaaaattga
     1321 atccttcaac cttgttccct gccatgaagg gttcttccga tttgaatatg aaaatagaac
     1381 ttgaaggttc cggtttcgat ccacttcaaa tgactgctta tgcaaaaatt caagtagaca
     1441 cctcaagtat ttccggatgg acgattcact cgttgcagtc caatctaaat tttcaacaac
     1501 agaatttctt tattgaaaat ttctcagttg aaacggcatc ggcacattta tctgccaatg
     1561 gcatttacag cataaaagga aattccgatg cttttctgaa cttgacgctc aacaacgctt
     1621 cagaaataat aaaattcacc ggcgtagaaa acttgaatgg aagaggcaaa attgccggac
     1681 attttcatgg agctccaaaa aaatttctta ccgatctgaa tttaaccttg tttgacacgc
     1741 aatttcaaac ttacagtgct gaggaagtta cagcacatat aactgcacaa atacccaatg
     1801 acaggaactt ttctgtatca ggtaatttga atgcaaaaaa tctgaatctc ggtggaatcg
     1861 aattacaaag cattcaactt gtatccaatg ccgattcaaa aaatatacaa ttgtcgtcta
     1921 ttgcagaaag cggggaattc aatacttcat ttaaatcggc aattcatctc gacaaccctc
     1981 tgctggttga attatccgat ttccgaataa attaccgaaa caacagttgg aaacagggta
     2041 ccgacactgc gctgttctac ttgagcgaaa ctgattatcg gcttgaacat ttaaaactaa
     2101 tttctgaaga tgctgatacc ttacaaactc tttcggccga aggaaaaatc agtaaaaatg
     2161 gagaacaaaa ttttcaaatt tccctctcta atttggattt tgccaaaata tccgaaacat
     2221 tttttcctga acaatcgcta caaggaaaaa taaattttaa tgtacaattg atgggcgaag
     2281 cttcttctcc ggtagtcgaa gcaaaatttc ggttaaacaa cttcgtactt caaaattatt
     2341 atgcaggcaa catgacaggg gcacttcaac tctcccaaca acagttgaac acaaaagtaa
     2401 acataaattt accggacagc ggaaaacttt ctgccgatgc aaaaattccg gtgaattttc
     2461 gtctggacag tatgcaattc aatattccaa gttacgaaaa tgaaaatttt gattttaatc
     2521 tttttgccaa taaaattccc cttacattac tgcaatcttt tcttcctgac ggacaatatg
     2581 aaggaatcat acaaacttcc atgcatgccg gtggaaacat taaagaaccc gaactgaaag
     2641 gaaaaattga aattacagac ggaaaaatca aaatgccgca atatggaatc aactatcctc
     2701 acttcatggc cggaatatcg atggataaca ataaaattaa aattgacact ttcctaataa
     2761 ccagtcgtga tggccgaatg aatataaatg gtaatatgtc ccctcccact tttttatcaa
     2821 aatctacaaa tgctccttca cctttggcaa atgttcaaat tcattttgcc aacttcagac
     2881 ctattgacca taaaaattat aacatggctg tatcgggcaa tgctttgctg aagggaaacg
     2941 ctgacagttt actcttttcg ggagatatta atatccctca agctgaaata aatcttcctt
     3001 cattgctgca actggtagga aaaacctccg cccctcaact ccccgaacct ctattaatta
     3061 aagagttgaa aaaaatgcaa cctgaagatt ctctggttat tacactgcga ccccgtaatg
     3121 aagaaagcaa aaaagaagaa acggattttt taaaaaaatt agaaggaaat ttacgtgtaa
     3181 aaattccacg taatacttgg ataaaaaatc aagatatgcg tgttgaactt tcaggtgatg
     3241 ttcaattaat gaaacacaaa gagttttttg aacttttcgg atcggttgaa gtgctgcgcg
     3301 gtcaatacaa tttactggga aaagttttca ttatcgagtc gggaacaatt tcttttcaag
     3361 gtggagaaaa aataaatcca ttactcaact tcactgcagc ctacaatttc agagactcaa
     3421 acagacaaca aaaaaaactg ctggcaaata tttcaggaga aatgatgtcg cctgaaataa
     3481 aattcacgat ggataaccag caaattgatg aaggagatgc actttcctat attctgtttg
     3541 gagcaaaatt ggatgccttg accagtggac aacgagaaaa tataaatcaa acttttgatg
     3601 cattgggatt gaccgaaagt cttgctgcat cgggactttc ttcgcaactt accaaacttc
     3661 tgagtaatac actgaatgta gattatatag aagtaaaaag caagggttca tttgaaagtg
     3721 gttcccttga agttggtaaa tacttgacca acaaaatttt tattaattac gttcgcaatt
     3781 ttggaaacta tcaacgcgac gaaaacttca gtgaatatga aattagagtc gagtacgaaa
     3841 ttctcaaatt tttgtttctt caattaacta cttcaccggt caaaaatgga atagacatag
     3901 ttattaaacc cacaaaaaaa taaggcttgt acccactttt aaagaaatgg agctgcttct
     3961 cattcctttc aatatgtaat gctggaaaaa ggtcgggaaa atataaaaaa atcgaaacaa
     4021 ttcatacaat aaattcatct aagcaatatt ctatctatgt cgaatttccg ccttatcctc
     4081 aacccataac acacttatgg cggctatcag caaaaaacaa cccgacatga taagtgcata
     4141 aatggcttgt gaatcaaaaa tccgtttcac caccattcca ccaaaaagtc cattcacaat
     4201 ctgcggaaca gtaatgaaaa tattgaagat acccatataa acacccgttt ttgcaggagg
     4261 aagcgatgga gcaagaatgg catacggcat cgacaaaatg ctgccccaag ctattccgac
     4321 accaatcatg gaaagtatca accattgggg atcattgata aagaaaattg aaagcaaccc
     4381 aacgccgcca cacattaagg aaaggctatg tgtccatttt ctaccaatag ttctggctat
     4441 agcaggtaaa acaagtgcat aaagcatggc aacaccattg taaacgccaa aaagtattcc
     4501 tacccaattt cccgcatcct gataagttgg ggaagaagta tcggtaacag gtaaaccata
     4561 aacatgctga gcaatggccg gcgttgtaaa aacccacatg ccaaaaagag caatccatgt
     4621 gaaaaattga gtaatggaaa gctgcttcat ggtttttggc atagttgcca tatctttgaa
     4681 aatatcggaa aattttggat tttttttctc cgttctttcc tcttccgatt caaatgcctg
     4741 caattcctcc ggcgagtatt ctttcgttgt aaacactgtc cacaaaattg taacaattag
     4801 cacgacagca ccaatataaa aagaaaaaat cacatttggc ggaacttgcc cttccggggc
     4861 agttttggct atgcccaacc actctgccag cacataaggc aaccatgaac caatgacagc
     4921 accaatccct atcagaaaac tctgaaccga aaatccgact gtagtttgtt cggcaggtag
     4981 catatcggca accagcgcac gaaaaggttc catagccaca ttaatggaag catccattat
     5041 cattagaaaa cctgcaccaa caaacatggc cggtaacaca tcggcaaaaa cacctgcatt
     5101 gggcattaat accaaagcga tagcagccaa aaaagcacct gccaaaaaaa acggtcgccg
     5161 cctgcccagt cgtgtccatg tgtgatccga ataatgacca ataatgggtt gaacaatcat
     5221 tcctgtaagt ggagcagcca accaaaacca ggaaagttgt tcaatatcag caccaaatcc
     5281 ttgcaatatt cggctggcat tggcattctg caacgcatag ccaaactgaa tacccaaaaa
     5341 tcccatactg agggaaagaa tcctaaaaaa acttaaatga ggtttcacct tttttctcgc
     5401 aaatgtaaat tcttgcatac ttttcaaatg tatgagtatt gaaaattttt aataaaaacc
     5461 attccgggaa attaatgtct caaaaccatt gaatcagcgc gtagtacctc tgattattaa
     5521 attggtttta attattttat tcgtatattt tccttgtgtc tcacccttga ttttggaaat
     5581 caacagttcg gccgcactga tgcccatttc gtaaccatgc tgttctacag tagtcaacat
     5641 agggtcgcag gcttttgcca attctccatc cgaaaaacca caaatggaaa tatcctgagg
     5701 aacatgcaac ttggccaatt ttgctgcata caaaatacct gatgcacaat ggtcgttaat
     5761 ggcaaaaaaa ccatcaggac gatcagggcg atctaaaatt tccggagtaa taatgatggc
     5821 ttcttcccgc gtatcgcaaa ctcgaatcag cgattcatcc acaggtaagc cgtatttgtg
     5881 gagcgcatcc agataaccgt ttctccgatt tttagaaatt tcgagatgaa gcggtgaagt
     5941 gtaaaaggca attttcttgc aacctgtttg aatcagatat tcaacggcag caaaagcccc
     6001 cgcataatca tccactacta ccctatctgt actgatgcca atgcaaatcc tatcgaaaaa
     6061 aaccaatgga atattgtgat caactatttc ctgaaaatgg ccgtaagtgg acgtattttt
     6121 agataagctg gccaaaatac ccgaaacacg tgctgaaatg agcgcatcaa catttcttac
     6181 ttccttttcg taattctcgc tcgactgaca aataatcacg ttaaatcctt ccttattggc
     6241 aacctgttca atacccgaca aaatagagga gaaaaaataa tgtataattt cagggacaat
     6301 cacacctatc gtgttgcttt tactcatacg aagattcata gccatcacat tgggacgata
     6361 gttgtattcc cgcgcatatt tattgacggc atcctttgtt tcctgactga tgtcgggatg
     6421 atttttcagt gcacgcgata ctgtggaagg cgatatattc aacgcccgtg caatatcctt
     6481 aatggtaatt tgtgagtttt tcatacccca aaaaaatttt caaagctcaa aaataaacaa
     6541 agcaatttaa aaaattaaat ttttttctga ttttttgagt tgcttgcgtt taaaaaaaat
     6601 caaaaaatca tacagacaaa tatacagtta tttttttatt ccaaattata aaaaatgaag
     6661 caaaaaatga aactaaaatt caaatgaatg aatcaattag cgaataaaca gtaaaaaaca
     6721 acataagtgt ttgtttaaaa gataaataat ttgcaaacgt ttgcataaaa tatttgcaag
     6781 tttttttctt ttgggcattg gaaatgtttt tttaacgtat atttttgcct atgtaatttg
     6841 aattaaaatt tagtttcaat ctttatatat gtttttcaac ctgataatgg attttaagta
     6901 aaagaatctc aattaataac cttaaaaaat aaaatgcatg atgaacaatc atttcaaact
     6961 caaaattttc acatttggaa tggctttact tttttctgtt gcagggaact tgtccaatgc
     7021 ccagcaaatt gccatttccg gaaaagtgat tgattcgaac acgggggatc ccattattgg
     7081 ggccaatatt cttgaaaagg ggactaccaa tggcaccatt accaattatg acggcgaatt
     7141 tactttaaat gttgcttctg atgccgttat tatagtaaaa tatgttggtt ataaacctct
     7201 ggaaattcct gttgcgggca aacgaaaatt tacaattaaa ctggaagaag atgttatcac
     7261 acttggagaa gtggtagcca ttggctatgg cacagtaaag aaaactgacg caaccggctc
     7321 cgttactgcc atcaaacccg atgccatgaa caagggacaa gttaccaatg cacaagacat
     7381 gttgcttggt aaaatcgccg gtgtgaacat tactaccgga ggcggtactc ccggtacggg
     7441 cgctactatt cgtattcgtg gaggttcatc cctcaacgcc agcaacgatc ctctcattgt
     7501 cattgatggt ttgccaatcg acaataacgg catcaagggt gtagccaatc agctgagcac
     7561 cattaatcca aacgatattg aaagctttac ggtactaaaa gatgcttctg ctaccgctat
     7621 ctacggatca agagcttcca atggcgtgat ccttattact accaaaaaag gttcaaaatc
     7681 gggaaaacct aaagtaagtt acgatggcaa tgtttcttta agtaccctta acaaaaggat
     7741 tgaagtttta aatggagatg aattcagagc ccttgttaac caagtttact caggagatgg
     7801 tcaaacagat attgatgcca gaaacgctat gggtacagaa aacacagatt ggcaggacca
     7861 aatctacaga aatgccttca gtcaggatca caacatcagt gtggtgggtg gaataaaaaa
     7921 tactccttat cgtgtttctg tgggttatac caatcaaaac ggtattataa aaacatccta
     7981 ttttcagcgt tataccggtt caattaatct tagtccttcg tttttcgatg atcatttgaa
     8041 atttaatctg aatggaaaag gcatgtttgc aaaaaaccga tttgccgatg gtggtgcagt
     8101 gggcgctgct ttgacaatgg atccaactca cccggtaaaa acagatgatc ccgaattcca
     8161 gcgattcgga ggttattggc aatggtttga aaaaaacaca aatggtacat tcaaatccat
     8221 caataccaat gcaacaaaaa atccgatggc attacttgaa caaaaaagag atgtttccaa
     8281 agcaaatgac tttattggga atgcagaagt agattacaaa ttcttctttt tgcccgaatt
     8341 aaaagctcac ttgagtctgg gtatagaaag ttcttatggc aagcaggatt tgcatatcga
     8401 ttccacagct acaagcgatt atccgtgggg ccgtacaggt tgggacaaaa ttgaaaaaac
     8461 caatcgtttg ttaaattttt atttccaata tgccaaagaa ataaacaaac atagttttga
     8521 tattatgggt ggctacgaat ggcaacatta ttatagggaa ggacaaaacg agtatcgaag
     8581 cttggaaaaa attgacagca acaacgatgg aattatagat gaaaatgatg gttattataa
     8641 ccatattaca ccggatcaaa gtctttggaa aacagaaaat tatttggtat ccttctttgg
     8701 tcgattgaat tattcccttg ccggaaagta cttgcttacg gcaactttac gcgatgacgg
     8761 ttcttcaaga tttgcgcccg aaaatcgctg ggctttattc ccatcggttg ctttggcatg
     8821 gaaaattagc gaagaacatt ttattagtga gcgaggtact ttttctgacc tgaaactcag
     8881 attgggttat ggaatcaccg gtcaacagga tatcagtcag ggcgattatc catatattcc
     8941 ggtttatacc atcagtaaag aacatgcttt atatccgttt gggaatgagt attattatac
     9001 ttcccgtcct gatgcataca acaaactcct aaaatgggaa caaaccacta cttggaatgc
     9061 cggtttggat gcaggcatat ttaataaccg cctcactggg tcgttggatt actatttcag
     9121 gaaaaccaat gatttgatca atgtagtttc tgtacccgcc ggaaccaatt tcagaaataa
     9181 agttatcagt aatataggat cccttgaaaa taaaggaatc gaatttatga tcagtggcaa
     9241 acctatctcc aataaaaact gtacgtggga tataagttat aatgtaacct acaataaaaa
     9301 cagaattaca aaattgacaa ctggcaacaa tgaaggatac tttgtagcga caggaggaat
     9361 tttccaaggc tccacgcagg ctcatactgt aggttatcct tccaatactt tttatgtgta
     9421 ccagcaaatt tatgatgaga acgggaaacc tatagaagca ggtgctccga aacctgatgg
     9481 aagcggagac gtttacaaag atatcgaagc ttttgttgac agagatggaa acaacacaat
     9541 cacggaagat gacaagtatt tttacaaaag cgctcatccc gattacacga tgggactttc
     9601 ttccaaattt atttacaagt cattcgacat tggattctca atgcatgcga gcataggcaa
     9661 ttatgtttac aatgctgtgg ctgcagaaag actaaacgtg ggagacaacg gcatttattc
     9721 attgggttat ctttccaaca aacccaaatc ggcattgaaa acaaactttc agggaacttc
     9781 tcccatcacc tatttgtcgg attattttgt ggagaacggg tcttttttgc gttgcgacaa
     9841 cataaccatt ggatatagtt tcaacaatct gtttaaagcc atctcaagcc taaggattta
     9901 tgctgctgta caaaatcctt tcctgatcac gggttattcc ggtctcgatc ccgaaatctt
     9961 tggcggcatc gacaataata tttatccgcg tcccatgaca acattactgg gtttatctgt
    10021 taacttttaa aaaaatgaaa aacatgaata ataaatattt aaacattttt ggcgtgttat
    10081 tggtggcatt tacttttcat gcctgcataa acgatttgga tgtaactccc attaatcctc
    10141 aggttactca aacctttaat caggatcaag tgtttgcaaa agtatatgcc gcttatgccc
    10201 tgacaggaca ggaaggacct gcaggcaaca atgacataga tattgtggat gaaggaagat
    10261 tttctctttt ccgctgcttg tggaattgca atgaactctc cacagatgaa gctgcttgtg
    10321 cttggggaga cgccgaagta atcgatttaa accaaaacac atggacagcc ggtaatttct
    10381 caaccgaagc actgtatgcc cgtttatatt ttattgtttc catcagcaac cattttttgg
    10441 aggaaacagc aaaaattacc gatgaaaaaa ccctgaaaca aagagcagaa gtgaaatttc
    10501 tcagagcact ggcttattac tatttaatgg ataattttgg gaatgtgcca tttactacta
    10561 ttgtaggcac aaaagcgcct catcaaattg aaagagccaa tttgtttgca tggattagag
    10621 gggagctgaa agccatcgaa aacgatatgt acaaaccgcg cgaggctcca tattatcgag
    10681 tagataaagc tgccaactgg cttctacagg caagattata tttaaactca gaagtatata
    10741 caggaacagc taaatgggac tctgctgcca tttatgcaaa aaaagttatt gattcgggat
    10801 accaattagc acctacttat cgacatctct ttatgggaga taatgccgga acattggatg
    10861 gctcaagcgt gaatagtgct cctcaggaaa taatttttcc tctcgcagcc gacggaatca
    10921 aaacacaaag ttggggatta tctttatttt tgataggttc aacctacaca tcggatatgg
    10981 gagattgggg aaccaatcaa ggatgggctg gtaatcgtgc tcgacaagat ttggtaaaaa
    11041 aattctttcc taacccacct tccgttattg caaaagatac aaatatgaca atgattactt
    11101 ttccatcata tgttaaagat gatagagctt tattctttag aaaaagcaga acattgtcta
    11161 tagtaaatct tggaaaattt aaggaaggat attcagtcat caaattttcc aatcgccggg
    11221 ctgacggagg aacaccacat gatcctctat ttgtagatat ggatgttcct ttcatgcgct
    11281 tggctgaagc ctatctgact tacgccgaag ccgtattgag aggagctcct gaaatagatg
    11341 ggtattcagc actggctgct gtaaatgctt tacgcactcg ggcacatagt ggcaaaattg
    11401 caactcagat taccctaaca gacgcagaga attttactac cggtaagttt aatattatca
    11461 acgaatgggc aagagaattt ttcttcgaag ggcgtagaag aactgattta atacggtttg
    11521 gaatgtttgg aggtgatact gactataaat gggaatggaa aggtggtgaa caggccggga
    11581 aaaatttcag tgcagatttt aatctcttcc ctatcccaac gtccgatttg aatgccaatt
    11641 ctaatttgat tcagaatccg ggttattaaa attcataata attttactgg ggtttgttta
    11701 taaagcaaac cccaatataa acagcataac aaattttcaa aaattaattt caaaaaaaat
    11761 tgacttttct attgtttatt tcgtttacaa tttgtacttt tgacaccaag gtgtgggtga
    11821 aatcacatca atatatataa atagttatta atcaatcaat tacttaaaat tttactatta
    11881 tgaaaaagtt atttactttt atgggagctc ttttgagctt gtttgcagtg gtacagatga
    11941 gtgctgctgc cgatttggta tttaatgtaa cggtgcctac tccaacttat gagtgctggg
    12001 tagtgggaaa ttttaacggt tggaacaaca accagtacaa aatgaccaag gtagatgata
    12061 cccactacac gattactatt ccagagtcgg aaattgtacc tggtctaact caaattgaat
    12121 acaaatacct gagtggaggt ggagattggg cttatgtaga aaaagatgct gacggcaatg
    12181 aaatttcaaa ccgtatttat acggatggta atgacgtagt tcaaaaatgg gctttggttt
    12241 ataatcctaa cgttgagcca ataccaatga cggttaccat tattgcacaa gttccggctg
    12301 atgttatcga attgtacatt gtgggaacat tcaacaattg gactattcca acagatacca
    12361 caaaaatgac ttttttggaa gccaatgaat cgggtaaggt tttctcggct actttcttta
    12421 ctgtggatgc caacaaattg caatttaaat tctgtgcagg cccagcttgg gattacgaac
    12481 aaacacaagg agccaattac acctatcccg acccaactca aaacacagca gcagttattg
    12541 ttgaatcttt caaaaaaatc tacgatccta ctaaagttgg cgatgttacc attatagcta
    12601 ccgttcctgc aggcaccgaa agggtatgga ttatgggcag ccatctgggc tggtattggg
    12661 ctaacttaca agaaggtaca atgaatccag atggaacctt tacatttgta gtcccggatg
    12721 taatgtcctt tgaatatcgt ttgtacaact ggtatgaacc agaatttggc tatccggaag
    12781 ttggagaagc tgatccgacc aaagatttgc caaatcgtac tgctcaatac gatccgacca
    12841 atccagccaa caataccatc aacataaccg tatggggttg gaaacagccg gcaccaagtg
    12901 cagtatcaaa tgtattcttc gacaattttc agccaaagat agtggacaga aaaattaagg
    12961 tagacaatgt aacttccagc gttgaattgt tcgatgtcat gggccgtcag cttgaaaaag
    13021 caaatgttag aggcacatac acaactagaa cactcaccaa cggcatttat attctgagag
    13081 tagatggtaa attcacagct aaaattgccg tgaagtaaat cttatcaaat agcaaataat
    13141 ttaagccttg aggtcgttaa aaccccaagg ctttttttgt ttttaaaatc caaacagcag
    13201 ccacaagaaa acatagatgt tttgaaatag agatatataa agtttaacaa cgttgttatg
    13261 tttatttcac ttcgaaggaa tgcgcagtag aaagaatcca tgaaaaagca ttatcagaaa
    13321 ttcttcaagt tgaataaatc ctacacataa atcctacaca acccattata ttgagcacag
    13381 actcactcgg cccaatacgc caaaaacaaa cggcatggtg gaaagagcca atggaatcat
    13441 taaaaaggaa accattctta aagacaatta cgcaaatatt tatattgagc atagtcgaaa
    13501 taaggaagaa atgaata
//
LOCUS       DAOSCH010000053        13489 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3092_length_13489_cov_7.114262, whole genome shotgun sequence.
ACCESSION   DAOSCH010000053 DAOSCH010000000
VERSION     DAOSCH010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3092_length_13489_cov_7.114262"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..530)
                     /locus_tag="PLZ54_05395"
                     /pseudo
     CDS             complement(129..530)
                     /locus_tag="PLZ54_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611509.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
     gene            629..1159
                     /locus_tag="PLZ54_05400"
     CDS             629..1159
                     /locus_tag="PLZ54_05400"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOJ66396.1"
                     /translation="MINTTAFHSACFGFASTPFGKCCVAKDDRGIFSLTFPFDEEFAI
                     NELFWKFPDKKFYHDDKEIGIMAQLIFQRNICPALNFYGSDFQKTVWEALLKIPFGGR
                     VSYEEIAISIGMPKAVRSVAHAIACNPIAFLVPCHRVIRKNGSIGEFRWGRERKVAML
                     EWEEKILNLEKFQEIE"
     gene            1337..1621
                     /locus_tag="PLZ54_05405"
     CDS             1337..1621
                     /locus_tag="PLZ54_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66397.1"
                     /translation="MKQVNSIYRFQFQIRAIKTSSLIEGFIIGVWCGDNRFLHIEFTR
                     NDAAQGKILVEKIHRLKTFINASSPSSHRLPTNFEEEEVYKDYCYSYSAL"
     gene            complement(2055..3164)
                     /locus_tag="PLZ54_05410"
     CDS             complement(2055..3164)
                     /locus_tag="PLZ54_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOJ66398.1"
                     /translation="MENKFVTLAIRTYKRAQMIKTTLEENGIETRIESVNLEHPEMSV
                     GVRIRIKENDLPRALKIVEEMEIAWEEKGETLKRAEKMILLPIDFSDQLPKIIDFGFY
                     FAESLNAKVELLYVYYSPPFTISSVNNEVNTYTLSDSELLRQIISNANREVTSMTNTI
                     NERIAKGELPNVPFHFELREGVPEDQIVDFCKKYQPALVIMGTRGKKISSELIGSVTA
                     EVIESCVSPVLALPVDMKINTPSEIKRIAFLTNFEQKDLIAIDNTISLFNWNSLEMFF
                     IHAAQKKEVWDEVMLAGIKEYFSKHYPKIITHYELIDSTETMKQIDDYLVKHDINLLA
                     FNSPRRNLIRRLFNPGLAFKMVLHSDTPLFVTHIS"
     gene            3526..4833
                     /gene="gldE"
                     /locus_tag="PLZ54_05415"
     CDS             3526..4833
                     /gene="gldE"
                     /locus_tag="PLZ54_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOJ66399.1"
                     /translation="MIAFHSFSVSAAIALLLSILLLMCSAIISGSEVAFFSLTPQMKN
                     EVEESNRKNAKNLMHLIGNPQQLLATILVGNNFVNVAIIMLLTYFTGSVFDFSTFPLL
                     GFIFETVIITFVLLLFGEIIPKVYASQYPEKMALLFTPFILVMQKVFSRVSKLLISST
                     AFLTRQIEKYKKAEISIDELSHALELTTDEQDDDKEMLEGIIKFGNIRVAEIMTSRVD
                     MIAVDTKTNFKDLLKIIIQTGYSRIPVYSENRDNIKGILYSKDLLPHLDKPANFRWQS
                     LIRQAYYVPETKKIDDLLSEFQKNKVHLALVVDEYGGISGLVTLEDILEEIVGDISDE
                     YDDEEKLYTKVDNHTYIFEAKILLNDFFKVTGINEEEFSKVTEEVETLAGLVLELKGE
                     MPAKNEMIEYGRYVFEILSVDNRRIRKIKLHMKDNFDRNKEEN"
     gene            4894..5499
                     /gene="gldD"
                     /locus_tag="PLZ54_05420"
     CDS             4894..5499
                     /gene="gldD"
                     /locus_tag="PLZ54_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOJ66400.1"
                     /translation="METKKIKYLQTIFYKTTLLIVITASLFSCGKNYVPRPYGYFRVD
                     LPPHNYRYLDTLQLPYRFEISSYAKVEKREAEGEKCWIDIKYPFLNAAIYCSYKPIHG
                     NLKELLEDTRQMAYKHSIRADAINEKLYQHPEKKVYGILYDLKGNTASSVQFFVTDSI
                     HHFFRGSLYFDNVPNKDSIAPMSDYIRQDIIRLMESFEWKQ"
     gene            5496..6140
                     /locus_tag="PLZ54_05425"
     CDS             5496..6140
                     /locus_tag="PLZ54_05425"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOJ66401.1"
                     /translation="MIHRKIETSGAKILVWQSTETFDELVTFFDKNETEELNTLHSEK
                     RKIEYLSVRLALKTLLNREIKICYNDDGKPFLSDYFAEIGISHSGQWIAVITHPTCKV
                     GIDIERFQPKISAISKRFLSKEELEKIFEKDENRLKKLTLLWSAKESLFKVIGKEAVD
                     FSRQLCILPFEMGKTGKIKALHIPRNKLYELNYELSEEYAMVYLIDKNVEPNKL"
     gene            6192..6944
                     /locus_tag="PLZ54_05430"
     CDS             6192..6944
                     /locus_tag="PLZ54_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOJ66402.1"
                     /translation="MKNLYKQILINREYNKKMLAVLLDPDRCQGKLMSSTIAALKLSA
                     PDFVFVGGSYVNSPIDSFIELLHEEVQIPVVLFPGNASQFSPKADALLFLSLISGRNP
                     EFLIGQHVTSALAIKQSGIEVIPAGYILIDGGKVSAVEYMSHTRSIPREKKEIVLATA
                     VAGELSGQHVVYLEAGSGAIQPVPKELINHVTSHLSIPVIVGGGMKTTQHLNDCFDAG
                     ADLAVVGNAVEQNPDLIVEFTNFVKNYNTIHR"
     gene            complement(7899..9089)
                     /locus_tag="PLZ54_05435"
     CDS             complement(7899..9089)
                     /locus_tag="PLZ54_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOJ66403.1"
                     /translation="MKKLTIISILILSILQLYAQKDTTAVDSFRFTTIKENKITPIKD
                     QASSGTCWSFSGLAFIESELLRMGKPETDLSEMFIVHHTYSDKADKYVRMNGTINFAA
                     GGSFFDDLYALKNYGIVPEAIMNGLNYGEEKHRHGELDALTEAYVKVIVTNPNKKLST
                     AWKQGFDGILDAYLGKCPEKFIWEGREYTPLTYFQSLGINPDDYVSLTSFTHHPFYTR
                     FAIEIPDNWRWGESYNLPLDEFMSVFDYAVNQGYTIAWASDVSEKGFTRNSIAVNPEI
                     KLEKLPGSDQAKWLNISPKEMEEKIYKIQGPQPEVQVTQESRQRGFDNYQTTDDHGML
                     IFGIAKDQNGTKYYLVKNSWGTDNKYKGIWYASESFVKDKTINILVHKNAIPKEIKTK
                     LGIK"
     gene            complement(9225..10688)
                     /locus_tag="PLZ54_05440"
     CDS             complement(9225..10688)
                     /locus_tag="PLZ54_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOJ66404.1"
                     /translation="MNSNDIRQLRPQQLWQNFYSLTQIPRPSGKKEVISQFLENFGKS
                     LGLETLRDEIGNVLIRKPATPGMEKRKPVILQAHMDMVPQKNSNVSHNFDTDPIQAVV
                     DGDWVRAQETTLGADNGIGLAAALTVLQSDNIAHPALEVLVTVDEETGMHGAFALKPN
                     FLKGEILLNLDSEDEGELYIGCAGGLDADITFRYQEVPVPESDVALKISLTGLKGGHS
                     GVDIHLQRANANKLLFRFLKKAVAEYEARLAWVEGGNLRNAIPREAFALITIPADGVD
                     DLIELLKDFENLFVEENQFVEDNIRLQVEKVKLPTGLMPEEIQDDLINGVTACPNGVF
                     RFIPEVFDVVETSNNLAVVKSDGSTIRLRCLLRSSVESRKEELASMVESVFSLAGAHI
                     DFSGGYPGWKPNLDSPILKVMTEVYEKQFGSKPKIKIIHAGLECGIIGMHYPDMDMIS
                     FGPTIRYPHSPDEKVNIASVEKFWDFLLATLQNIPEK"
     gene            complement(11373..13016)
                     /locus_tag="PLZ54_05445"
     CDS             complement(11373..13016)
                     /locus_tag="PLZ54_05445"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOJ66405.1"
                     /translation="MRKLLLLIVSVLAYFNSMACTNFLVGKAATVDGSTLISYSADSY
                     FMFGSLYYSPAATYPDNAMLDIYEWDTGEYLGKIKQAERTYSVVGNMNEFQVSIGETT
                     FGGRPELVDTTAILDYGSLIYIALQRSRSAREAIKVMTDLVEQYGYRSEGESFSIADP
                     NEVWIMEMVGKGNRNKGAVWVAQRIPDDCVSAHANQARITTFPFDDKNNCLYAEDVIA
                     FAREKGYFKGKNKDFSFSDTYAPLDYLGLRICEARVWTFFRKVDPSMDSYISYIKGET
                     AQRMPLWIKPNRKISAQDVKEFMRDQYEGTELDITKGIGAGPFHSKLRCSPLTFYVDS
                     VEYVHERPVATQQTGFSFVAQMRSWLPDYIGGILWFGVDDAASSIYVPMYCGINSVPE
                     CYRTGNGSLVEYSPTSAFWIYNQVANFAYSKYSFMMQDIKKVQSKWEQDFNSLVPTID
                     KVALGMSEESAKSFLTNFSNYQAEASTAAWKKLGEYLLVKYMDGNVKMEKDGKFLQND
                     KGIPPGIIRPGYPEDFLRMMVKENPGLRVKTAEELKNRK"
     gene            complement(13285..>13489)
                     /locus_tag="PLZ54_05450"
     CDS             complement(13285..>13489)
                     /locus_tag="PLZ54_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66406.1"
                     /translation="SAEVEAKFAWGQKKKNTQTRLTFVSNYLVKTVQRIGGQTTFVEK
                     RSKKGKNFSLNFSIILFTDYLFY"
BASE COUNT         4127 a         2547 c         2484 g         4331 t
ORIGIN      
        1 cttttgtaac aaaaataact tttatattgc aaattattgt aaaatatcga aacaacaaaa
       61 aatttaatgc ctctcttcaa aaaaaatctt attttttctc ttttttgaaa aaatcaggtt
      121 ttaagtgggg attctttttt tacttcttca attactgttc tttccagttt gtcagtcaat
      181 gtgcgtccat aatgcttaat cagctgataa taaatttctt caaatacccg ataatcccta
      241 ttcctgttgc catcgctcat cgttgatttg accggattct gctttaatcc caaatcagcc
      301 ataaaggtac tactgattga taattagcaa cttatatctg acaaagtata gcatttacca
      361 agctgtccga aacttaatgc caccaattgg tcgtatgtct tgtatttatg gcaacctttg
      421 tcgctttgat atttttgtat agaattacga agtatagatc ttggaattaa gtctaaaatt
      481 tgtcggataa ccggtttttt gttattttta ctgcgcctgt ggagtttcat tatttctgta
      541 tgttttgttt cactacaaaa atatgaaatt ctcaggcatt tttaatttct aaagtttcgg
      601 atagtaatga acttttcaca tttgcttgat gataaataca acagctttcc atagtgcttg
      661 ttttggtttt gcttcgaccc cttttgggaa atgttgcgtg gctaaagatg atcggggaat
      721 tttttcgctt acttttcctt ttgatgaaga atttgcaata aatgaattgt tttggaaatt
      781 tccagataaa aaattttatc atgatgataa ggaaatagga ataatggctc agctcatttt
      841 tcaaagaaat atttgtccag cattaaattt ttatggatca gattttcaga aaaccgtctg
      901 ggaagccttg ctgaagattc cttttggagg tagagtttct tatgaagaaa ttgccataag
      961 tattggcatg cctaaagcag ttcgctcagt agctcatgct atagcgtgca atccgatagc
     1021 atttctggtt ccctgtcata gagtgattcg taaaaacgga agtattggag aatttcgttg
     1081 gggaagggaa agaaaagttg ctatgttgga gtgggaagag aaaattttaa atctcgaaaa
     1141 atttcaggaa atcgagtaaa tgttctcggt aaaaaactct tttttagatt ttttatcgca
     1201 acacgatcac gtaacctttc tgtaaaagac tttgcaggtg tcattttttt aagcacagaa
     1261 ttcgcagtag gaaaataaag ttttaatttt gtaaaaaact caaatgcccg gcaatataaa
     1321 atgacgttta tgaactttga agcaagtaaa ctcaatttat cggtttcagt ttcaaatccg
     1381 agctataaaa acatcttctc ttattgaagg attcataatc ggtgtatggt gtggagacaa
     1441 tcgattttta catatagaat ttacccgaaa cgatgcagca caggggaaga ttttggttga
     1501 aaaaatacac cggctcaaga catttataaa cgcttcttcc ccaagttcac acaggttacc
     1561 aaccaatttt gaggaggaag aagtttacaa ggattattgt tattcttatt ctgctttgta
     1621 accaccatga aactttcagc accagagaga tatggagaat ataccgaaga agagaaaagt
     1681 gctgaagaca aaataaagaa attaaaatat gactttgtct ttgacagttt taacctaaaa
     1741 gtctttttta tgtaactgaa gcagctttaa tttttgtgag gatagcccat catcttatgt
     1801 cgctgtttag aatgcttatt tggcagggaa aggcgcaaaa aactatctcc atactgctga
     1861 tttgaacttt tgccgtaagt gcatattttg aaaaatcaac agcagaccgt ttctcaaaat
     1921 cgctttaaaa taaaaaagcg agtctggttt ttccagagtg tggaacagtt gttctgaact
     1981 tgactttccc tttaaattgg ctaatgaata atctgggtta aaatctaaca acaaacattg
     2041 caaattgaaa atgtttatga aatgtgcgtt acaaacaaag gtgtatccga atgtaaaacc
     2101 attttgaaag ctaaaccggg attaaatagc cgtcggataa ggttgcggcg tggagaattg
     2161 aatgccagca aattaatatc gtgtttgacc aaataatcgt caatttgttt catggtttcg
     2221 gtagaatcta ttaattcata atgagtaata attttagggt aatgtttgga gaagtattct
     2281 tttattcctg cgagcatcac ttcgtcccat acttcctttt tttgtgcagc gtgaataaag
     2341 aacatttcca gggaattcca attaaaaagt gaaatggtat tgtcaatggc tataaggtct
     2401 ttttgttcga aatttgtcaa gaaagctatc cgtttgattt cggaaggtgt attaattttc
     2461 atgtcaacag ggagagccaa taccggagat acgcaagatt ctataacttc tgctgttaca
     2521 cttccgataa gctcactgct gatcttcttc cctcgtgtcc ccatgatcac caaagcaggt
     2581 tgatattttt tacaaaagtc aacaatttga tcttccggta cgccttccct gagttcaaag
     2641 tggaaaggga cattaggcaa ttctccttta gcaattcgtt cgttaatggt attggtcatg
     2701 gaagttactt cacgattagc atttgaaatg atttgacgga gtaattcgct atcgctcaga
     2761 gtataagtgt tgacttcgtt gtttaccgaa gaaattgtaa aaggagggct atagtaaaca
     2821 taaagcagtt cgaccttggc atttaaagat tctgcaaaat aaaaaccaaa atcgataatt
     2881 ttaggcaact gatcagaaaa atctataggc agcagtatca ttttttctgc tcttttaaga
     2941 gtttcccctt tttcttccca cgcaatttcc atttcctcta caattttcag agcacgggga
     3001 aggtcatttt ctttaattct gatacgaaca cctaccgaca tttcgggatg ttccagattc
     3061 acactttcta tccgggtttc tatcccgttt tcttcgagcg ttgttttgat catttgagcc
     3121 cttttgtaag tgcgaatagc gagcgttaca aatttatttt ccatgatttc taagatttta
     3181 gtttttaata ggaaactttc ctttgtccta tcctttcatt ttcgtgtaaa gttaataatt
     3241 tttatgatat gtatcatcca gtttttgttt tttttctaaa aacagaaatt aaccttatat
     3301 ttgcatttaa aataaacttt tttaaaacag ctttaaagcg tttcccttta cataaaacgc
     3361 ttctataagc aaatttgttt taagaatttt tttgtttgtt aaggcttagt tgcaagaaat
     3421 tatatttgaa atagacaagt tctttggtat gagagtaaaa aaatttctac cactaactgt
     3481 tttagaatta agataataac tattctttta tcacatatta atgcaatgat agcatttcat
     3541 tctttttctg tttcggccgc aattgcactt ttgttgagta tattattgct aatgtgttcg
     3601 gcaattattt caggttcgga ggtagctttt ttttctctta ctccacagat gaaaaatgaa
     3661 gttgaagaga gcaacagaaa aaacgcaaaa aatctgatgc acttaattgg caatcctcaa
     3721 cagctgttgg caaccatttt ggttgggaat aattttgtta atgtggccat tatcatgttg
     3781 ctgacgtatt ttacaggctc cgtgttcgat ttttctactt ttcctttgct gggatttatt
     3841 ttcgaaacgg tgatcataac ttttgtattg cttctttttg gtgaaatcat acctaaggtt
     3901 tacgcatcgc aatatcccga aaaaatggca ttattgttta ctccatttat tttggttatg
     3961 caaaaagtgt ttagtcgggt ttccaaattg ctgatttctt ctactgcttt tcttacccgt
     4021 cagatagaaa aatataaaaa agccgaaata tcgatcgatg aactctctca tgcgctggaa
     4081 cttaccactg atgagcaaga tgatgacaaa gaaatgctcg aaggtattat aaaatttgga
     4141 aatatcaggg tggcggaaat tatgacttcg cgagtggata tgatagctgt agatacaaag
     4201 acaaatttca aggatttatt gaaaattatc atccaaacgg gttattctcg tattcctgtt
     4261 tattctgaaa acagagacaa tataaagggc attctttaca gtaaggattt gctgccccat
     4321 cttgacaaac ctgccaattt cagatggcaa agtttaatta ggcaagctta ctacgtaccc
     4381 gaaacaaaaa aaattgacga tctgctcagc gaatttcaga aaaacaaagt tcatcttgct
     4441 ttggtggtcg acgaatatgg tggaatttcc ggactggtta cgctggaaga tattttggaa
     4501 gaaattgttg gcgacatcag tgatgaatac gacgacgaag aaaaacttta taccaaagtg
     4561 gacaatcaca cctatatttt tgaagcgaaa attttgctga acgacttttt taaagttacg
     4621 ggtatcaacg aagaggaatt ttcaaaagtt actgaagaag tggaaacttt ggccggcttg
     4681 gtacttgaac tgaagggaga aatgcctgca aaaaatgaaa tgatcgaata tggacgttac
     4741 gtttttgaaa tcttgtcggt ggacaaccgc agaatccgaa aaattaaact tcatatgaaa
     4801 gataatttcg atcgcaacaa agaagaaaat taaaacaact ttttgatttg aatatcaagc
     4861 tgtttttttg atgaaaaaac aatcggcaac ttcatggaaa caaagaaaat caaatacttg
     4921 caaacaatat tttataaaac gacgctcctt atcgttatca cggcttcttt gttctcctgc
     4981 ggaaaaaatt atgttccccg tccctatgga tatttcaggg tagatttacc tcctcacaat
     5041 tatcgttatc tcgatacgct tcaactacct taccgatttg aaatttcgag ctacgccaaa
     5101 gtggaaaaaa gagaagctga aggagaaaaa tgctggatcg atataaaata tccttttctg
     5161 aatgcggcca tttactgcag ttacaaaccc attcatggaa atttgaagga attgttggag
     5221 gatacacgac aaatggctta taaacacagc attcgtgccg atgccattaa tgagaaactt
     5281 tatcagcatc ccgaaaaaaa agtttacgga attctttacg acttgaaagg aaataccgct
     5341 tcttcggttc agttttttgt aaccgacagc attcatcact ttttcagggg ctcactttat
     5401 ttcgacaatg tacccaacaa ggattccata gctcccatgt cggattacat tcggcaagac
     5461 attatcaggt tgatggaaag ttttgaatgg aaacaatgat tcacagaaaa attgaaacat
     5521 ccggcgcaaa aattctggtt tggcaaagta ctgaaacgtt cgacgagctg gtgactttct
     5581 ttgataaaaa cgaaactgaa gagttaaaca ccttacactc cgagaaacga aaaatcgaat
     5641 atctttcggt acgtttagca ttgaaaactt tgctgaacag agaaataaaa atctgttaca
     5701 acgacgatgg caaacctttt ctttcggatt attttgccga aatcggtatt tcccacagtg
     5761 gtcaatggat cgccgtgata acacatccta cttgcaaagt tggaattgat attgaacgct
     5821 ttcaacccaa aatatcggca atttccaagc gatttttgag caaagaggaa ttggagaaaa
     5881 tctttgaaaa agatgaaaac agacttaaaa aactgacctt gttgtggtcg gcaaaggaat
     5941 cgctgtttaa agttattgga aaagaagcag tcgatttttc tcgtcagttg tgtattcttc
     6001 cattcgaaat gggaaaaacc ggaaaaatca aagccttgca cattcctcgt aataagttgt
     6061 atgaactcaa ttacgaatta tcggaagaat atgcaatggt ttatcttatt gataaaaatg
     6121 ttgagccaaa caaattatga aaatttatca cgataaagga taaaagacaa aaatcataca
     6181 caaagagaat catgaaaaat ttgtacaaac aaatattgat aaatcgggaa tacaacaaaa
     6241 aaatgctggc cgttttgctc gatccggatc gttgtcaggg aaaactgatg tcgtcaacca
     6301 ttgctgcatt aaaactttcg gctcccgatt ttgtatttgt aggtggcagt tacgtcaatt
     6361 ctccgataga ttcgtttatt gagcttctcc atgaagaagt acaaattcct gttgtacttt
     6421 ttccgggcaa tgcttcccaa ttttctccga aagccgatgc attgcttttc ctttcactaa
     6481 tttcgggcag aaatcctgaa tttttaatcg gacagcatgt aacctcggcg cttgctatca
     6541 aacaatcggg aatcgaagtg attcctgccg gttacattct tatcgacggg ggaaaagtta
     6601 gtgccgtaga atacatgagt catacacgtt ccattcctcg cgaaaaaaag gaaattgttc
     6661 tggcaacagc ggtggccggc gaattgtcgg gtcagcatgt tgtttatctg gaagccggca
     6721 gcggagcaat tcaacccgtt ccaaaagaac tgattaacca tgtaacatct cacctttcca
     6781 taccggtgat tgtaggcgga ggcatgaaaa ccacacaaca tcttaatgac tgcttcgacg
     6841 caggtgccga cttggctgtc gtgggcaacg ctgttgaaca aaatcccgat ttaattgtcg
     6901 aatttaccaa ttttgtaaaa aattataata caatacatcg gtaaataatg ggtcagtaaa
     6961 gtggtaatta ataaattaaa caaaaatttt tatgataaac ttgtaaaaga gcccattgag
     7021 tttgctgcaa ttgctgtttg actctacatt ttatttttaa cccaactttc cattcattaa
     7081 gcggaactca ttactaaaca agtaaatata ctgtctggcg gatgaatttg ggtactacaa
     7141 atttttcgta gtttgaatgg ttgttgtttg tactttagct cttcgtaaat ttctgcagct
     7201 tgagtttggg gcatagtaca atattctaaa agctacttgt tctccaagtt tattttcggc
     7261 agtagtggtc agcacttttt cagttgacct gattcttgtt attttggtca taaagtactt
     7321 gatgccatgc cttttgagtc tgtttccaag aggtcactaa ttgatctacc cgctttcgtt
     7381 ggttggcatc caattcgatg atgaagcccg aaaccaagag aatacgactg cgaactactt
     7441 ccgctatatc gcggtacttt tccttgatac gaaaataact cttgggcatt tttgtatccg
     7501 gtctgtggca tactgataaa tcgaaattta ttccgcaaag ttctgtaatt caaattaaac
     7561 caccaaaatc cagggatact acaaatgtgt tctcagaaac aacaactatt ggaatccaat
     7621 atgttacaat tttaatcgac tgaatttgac tatcaaacta accaatatca gcctgttttt
     7681 aactaaactt taacgttatg ttattcatgt ttggaaagtt gggttaatct tactttcaaa
     7741 aaaataaaaa gaatttcgcc caaagaattt cgtaaaaact ctttgggcgc ttagtaacca
     7801 cgaccattga tttgtttcat caatcaaggc catttgttct caaatcttat tttgcagcaa
     7861 actcagtaac agcaaaatgt ctggataaat ccgtcgattt acttgatgcc cagtttagtt
     7921 ttgatttcct ttggaatagc attcttatga accaaaatat taatggtttt gtcttttaca
     7981 aatgattcgc tggcatacca gatacctttg tatttgttat cggttcccca cgaatttttt
     8041 accaggtaat attttgttcc gttctgatct ttggcaatac caaaaatcag catcccgtga
     8101 tcgtcggtag tctgataatt atcgaatcca cgctgacgac tttcctgtgt tacctgcact
     8161 tcgggctgag gtccctgaat tttgtaaatt ttttcttcca tttctttggg tgaaatattc
     8221 agccattttg cctgatccga accgggcagc ttttccagtt ttatttcggg attaacggca
     8281 atgctgttgc gtgtgaaacc tttttcgctc acatccgatg cccaggctat agtatagcct
     8341 tgattgactg cataatcgaa aaccgacatg aattcgtcca gcggcaagtt atacgactcg
     8401 ccccagcgcc aattatcggg aatttcgatg gcaaatcggg tgtaaaaagg atgatgcgta
     8461 aatgaagtga gcgaaacata atcgtcggga ttgattccca atgattgaaa ataagtaagc
     8521 ggtgtatatt ctctgccttc ccagatgaat ttttcgggac attttcccag ataagcatcc
     8581 agaattccat caaagccctg cttccatgca gtagataatt ttttgttcgg gttggtaacg
     8641 attaccttta cataagcttc cgtcagagca tccaattcac cgtgcctgtg cttttcttcg
     8701 ccatagttta gcccgttcat aatggcttca ggaacaatgc cataattttt caacgcatac
     8761 aaatcgtcga aaaaggaacc tccggcagca aagttaatgg ttccgttcat gcgtacatat
     8821 ttgtcggctt tgtcggaata ggtatgatgc acaataaaca tttcggacaa atccgtttca
     8881 ggtttcccca ttctcaacaa ttccgattct ataaaagcca agccggaaaa actccagcaa
     8941 gttccggagc ttgcctggtc tttgattggc gtgattttat tttctttgat ggtggtgaag
     9001 cgaaaactgt ccaccgcggt cgtatctttt tgggcgtaaa gttgtaaaat gcttaaaatc
     9061 agtatagaaa ttattgtcaa ttttttcatc agaatataat ttatttttta attgtctgat
     9121 ggaacccaca tgtctgaata ttatattcat tcgtgtttgt gtatgcaata catgtgggtt
     9181 tcatatgttt tattgaaatg agttttttat tttttttctg aaatttattt ttccggaata
     9241 ttttgcagtg ttgcaagcaa aaagtcccag aatttttcga cggaagcaat attgactttt
     9301 tcatcgggcg aatgcgggta tcgaatggtt ggtccgaaag aaatcatatc catatcggga
     9361 taatgcatgc caataatgcc acattccaaa ccggcatgaa tgattttgat tttcggtttc
     9421 gaaccgaatt gtttttcgta aacttcggtc attaccttta aaattggcga gtccagattg
     9481 ggtttccagc cggggtaacc gccggaaaaa tcaatgtgcg cacctgccag tgaaaagacg
     9541 ctctctacca ttgaagctaa ctcttccttt ctcgattcta cggaacttct aagcagacaa
     9601 cgtaaccgaa tggttgagcc atcagatttt accactgcca ggttgttgga cgtttccact
     9661 acatcgaaaa cttcggggat gaatcggaaa acaccattgg ggcaagctgt tacaccatta
     9721 atcaggtcat cctgaatttc ttcgggcatc aagccggtgg gcaattttac cttctccact
     9781 tgcaatcgaa tattgtcttc cacaaattgg ttttcttcta caaacaaatt ttcaaaatct
     9841 tttaacagtt caattaaatc gtccacaccg tcggcaggaa tagtaatgag agcaaaagct
     9901 tcgcgtggaa tggcattacg caaatttcca ccttccaccc aggccaggcg agcttcgtat
     9961 tcggctacgg cttttttcag gaaacgaaac agcaatttgt tggcattggc tcgttgcaag
    10021 tgaatatcca caccagaatg tccacctttc agccccgtca gagaaatttt taaagcgaca
    10081 tcgctttcgg gaacaggaac ttcctgatag cggaacgtaa tgtcggcatc caatccgccc
    10141 gcgcaaccga tatacaattc tccttcatct tccgaatcga gattgaggag aatttctccc
    10201 ttcagaaaat ttggtttcaa agcaaaagca ccatgcatgc cggtttcttc gtccactgta
    10261 accagcactt ccagcgccgg atgagcaata ttgtccgatt gcaaaacagt aagtgctgca
    10321 gccagcccga taccgttgtc ggctcccagc gtagtttctt gagctcttac ccaatcgcca
    10381 tcaacaaccg cttgaattgg atcggtatca aaattatgtg aaacattgct gtttttttgt
    10441 ggaaccatgt ccatgtgtgc ctgcagaatg acgggttttc ttttttccat tccaggcgtc
    10501 gccggtttcc gaattaacac attgccaatt tcatcccgca aagtttccaa accgagtgat
    10561 tttccgaaat tttcgaggaa ttgactaata acttcttttt tccctgaagg acgcggaatc
    10621 tgtgtcagac tgtaaaaatt ttgccacaat tgctgcggac gaagctgacg aatatcattg
    10681 ctattcataa tatgtttatt attagagttt tgtaaattct gaatatttat tccaattttt
    10741 taagctacaa atatgctcaa attcttcaga taaatgaaca gttgaagaga ttttttacaa
    10801 aatattttac agattggtaa aaaaaagaag ttgaaaatca ccattatcag aaaaactttc
    10861 atttagaaga tatttatact tgtaaccctt ctgaataaag catattgttt tttcaaaaga
    10921 gacttcacct caaaattgcg ggttcaaggt taactgatct gtgaaaggtg tagcctcctg
    10981 attctttttt aaaggtcttt ttgttcccag atcaattcat aaacatccca caagctgatc
    11041 aaatctatcc gtactaatgt tgccaaatgg aaaaggtctt tttcatatcg gctctttggg
    11101 gggggtcgtc caactccttt gagaatattg ctaaacagcc ttattgtgtt agaatcaatc
    11161 aaaagaactt cataaaattt aatctgaaag tcccgccgta agaccaaatt tatttccgct
    11221 ttttgcctct atatttttta tgtttgtggt aaaacaaaaa aattatgtgc cggtgaagtc
    11281 atcaaatttg ggtaactttc ctttttatta taacttattt tgtttttttc ggacagcagg
    11341 gattttcagg ataagaaaaa gtaagaggag gtttattttc ggttttttaa ttcttctgcc
    11401 gttttcaccc tcaaccctgg attctccttc accatcatac gcaaaaagtc ttcaggataa
    11461 ccggggcgaa taattccggg aggaatacct ttgtcatttt gaagaaattt tccgtccttt
    11521 tccattttaa cgttaccatc catgtatttt acaagcaaat attcacccag ctttttccat
    11581 gctgccgttg aagcttctgc ctgataattg ctgaaattgg tcaaaaacga tttagccgat
    11641 tcttcagaca tccccaaagc caccttgtca atagtgggaa caagtgaatt aaaatcctgt
    11701 tcccatttcg actgaacttt tttaatatcc tgcatcataa aagaatactt gctgtaagca
    11761 aaattggcaa cctgattgta aatccagaat gccgaagttg gggaatattc taccagactt
    11821 ccatttcctg tacgataaca ttccggtaca ctgttgatac cgcaatacat gggcacatat
    11881 atggaactgg cagcatcgtc aacgccaaac cacaatattc cgccaatata atcgggcagc
    11941 caactacgca tttgagccac aaacgaaaaa cctgtttgtt gagtagctac aggacgttcg
    12001 tgcacatatt ccacactgtc cacataaaaa gtaagaggac tgcatcgcaa cttgctgtgg
    12061 aaaggtccgg caccaattcc tttggtaatg tccaattcgg ttccttcgta ctgatcacgc
    12121 ataaattcct ttacatcctg agcactgatt ttacggttgg gttttatcca cagaggcatc
    12181 cgctgagcag tctctccctt gatgtaagaa atataactgt ccattgacgg atcaactttt
    12241 cggaaaaaag tccaaacccg cgcttcacaa attctcagtc ccaaataatc caaaggagca
    12301 taggtatccg agaaactgaa atccttgttt tttcccttaa aataaccttt ttcacgagca
    12361 aaagctatga catcttccgc ataaagacaa ttgtttttat catcgaaagg aaaagtagtg
    12421 attctggcct ggttggcatg agccgaaaca caatcgtccg gaatacgctg tgcaacccat
    12481 actgcaccct tattcctatt tccttttccc accatttcca taatccaaac ttcattcggg
    12541 tcggcaatgg aaaaagattc tccttcactt cgataaccat attgctcaac caaatcggtc
    12601 attaccttta tggcttcacg ggcagatcgg gaacgttgca acgcaatata aatcaaactt
    12661 ccataatcga gaattgcagt tgtatcgacc agttcgggac gtccgccaaa agtagtttcg
    12721 ccaattgaaa cctgaaattc attcatattg ccaacaacgg aataggttct ttcggcctgt
    12781 ttgatttttc ccaaatactc acccgtgtcc cactcataaa tatcgagcat ggcattgtcg
    12841 ggataagttg ctgccggtga atagtacaga gaaccaaaca tgaaataaga atcggcagag
    12901 taactgataa gggttgaacc atcaaccgtt gcagctttcc caaccagaaa attggtacaa
    12961 gccattgaat tgaaataagc taatacggaa acaattagaa gaaggagttt tctcattttt
    13021 gtgttaaatt ttaagaatta caaatttagt ttatataaat gtaaattcaa aatcatcagg
    13081 gagagaggac acaagaaact tatagatcaa aaagaaggct aaaatctact gagaaatcaa
    13141 ttctaatatt tttcgcatca aatcaatggt tagcaataaa aaatgcagta tatggaagta
    13201 aaatattttg ttaaacaatt tgttaatagt caaagtatta attctataat aacattttta
    13261 agatagtgat acctcggtaa ataattaata aaataggtaa tctgtaaaga gaatgataga
    13321 aaaattcaag gaaaagtttt ttcctttttt tgatcgtttt tcaacaaaag tggtttgacc
    13381 ccctattctt tggacagttt taactaaata gtttgagaca aaagttagcc tcgtttgtgt
    13441 gtttttcttt ttctgacccc aagcaaattt agcttcaact tccgcactt
//
LOCUS       DAOSCH010000054        13417 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3121_length_13417_cov_6.078057, whole genome shotgun sequence.
ACCESSION   DAOSCH010000054 DAOSCH010000000
VERSION     DAOSCH010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13417
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3121_length_13417_cov_6.078057"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="PLZ54_05455"
     CDS             <1..403
                     /locus_tag="PLZ54_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOJ66407.1"
                     /translation="GMARIDEVEFNGQKIVPIQFLKAVLPNPGDLGENYKGWTSIGCR
                     IRGIKDGKEKTYYIYNNCSHEAAYKETGTQGVSYTTGVPAMIGAMMFLQGKWKKPGVF
                     NVEEFDPDPFMEQLNKQGLPWHELINVDLEL"
     gene            complement(473..649)
                     /locus_tag="PLZ54_05460"
     CDS             complement(473..649)
                     /locus_tag="PLZ54_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66408.1"
                     /translation="MQKVTTSDNSADEVDSGQQKKTKVMCPQMETIERILQFAAAYRA
                     EKINENQYAELLWN"
     gene            complement(766..1359)
                     /locus_tag="PLZ54_05465"
     CDS             complement(766..1359)
                     /locus_tag="PLZ54_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOJ66409.1"
                     /translation="MKDFNQFTDDELVKLYKEGNNQAFEILLKRYQSKVYTYIFLIVR
                     NRELTEDIFQDTFIKAIATIQQDRYVESGKFLAWINRIAHNLIIDYFRREKNENTFSV
                     EGINFDILNCTRFSEKSMEDVLSNEQVFVDVVHLVEKLPLSQQAVVKMRFFENLSFKE
                     IAEKTNVSINTALGRMRYALINLRKIASEKNISLEIK"
     gene            complement(1536..2720)
                     /locus_tag="PLZ54_05470"
     CDS             complement(1536..2720)
                     /locus_tag="PLZ54_05470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOJ66410.1"
                     /translation="MHVVFLKPKKEESVRRFHPWVFSGAIQKIEGQPSEGDLVKVLDS
                     QGNFLGIGHFQQGSIMVRLLSFQEEPIDENFWKNRIAEAYQMRRALGLMIENRNNCYR
                     LVHGEGDGLPGLIVDVYGNTAVMQAHSVGMHKIRNVLAKCVVECVPEIQYLYYKSEAT
                     LPFRNENAPEDGYLIGGDSEQFVVMENGLKFFVDWQHGQKTGFFLDQRENRVLLERYA
                     SDRIVLNMFCYTGGFSVYALRGNAKLVHSVDSSAKAIELTDKNVALNFSDNRHQSFAE
                     DAFKYLQLYEKRAPFYDLIILDPPAFAKHRDAVPNALKGYRKLNRKAFEIIRPGGIIF
                     TFSCSQAITREQFRQVVFSAAAESKRNVRILHQLSQGTDHPINIYHPEGEYLKGLVLW
                     VE"
     gene            complement(2772..3476)
                     /locus_tag="PLZ54_05475"
     CDS             complement(2772..3476)
                     /locus_tag="PLZ54_05475"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004338754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOJ66411.1"
                     /translation="MYSPKISKEEINQLPVVSFPGEIVLVDKPEMVAPAIDELRKNLV
                     VGIDTETKPSFKSGTSNKVSLLQLSELERCFLFRLNLIDFPQELQDFLNDENIKKVGL
                     SLHDDFRLLNKRQNFHPANFVDIQNIAKSYGIMELSLQKIYAIIFGKKISKSQRLSDW
                     EKPQLTEKQLRYAATDAWASLQIYLQLMNEPQLTPKQIEKLVQENTPHKQPETSENAE
                     TTNSDLKPDAHSEKLS"
     gene            complement(3484..4125)
                     /locus_tag="PLZ54_05480"
     CDS             complement(3484..4125)
                     /locus_tag="PLZ54_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOJ66412.1"
                     /translation="MSIDELPDHIVYSANVIEFVTVVAETCLFLEHTSEMGKDEFVEK
                     SLKLLPLLYLKASVLDIPEPVYENLPERFVTEEDYQFVREQIEQLLGNDDVYLEVFHP
                     DMAISDTPVAAFISENMSDIYQEIKDFAANYQTADVDIMSDALSECLTAFGEHWGQKL
                     LNAMRALHALKYNPQDDTEWEEMHQPDEKQLTRDSFLDFQHTAENDDVSPFLK"
     gene            4354..4593
                     /locus_tag="PLZ54_05485"
     CDS             4354..4593
                     /locus_tag="PLZ54_05485"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOJ66413.1"
                     /translation="MEDKIVTLKNYDTIVKAMFDQEILRANHIECFINNEQLVELYPM
                     FADIDEGLKIVVFEKDLEKAQKVLEEYHSSIEENV"
     gene            complement(4590..5108)
                     /gene="folK"
                     /locus_tag="PLZ54_05490"
     CDS             complement(4590..5108)
                     /gene="folK"
                     /locus_tag="PLZ54_05490"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOJ66414.1"
                     /translation="MSLIFLGLGSNLGEKEKNLQDAVMMLKMEIGSLLSQSSFYISKP
                     FGFSSENDFLNAVVLMETHLSPCELLDKTREIEQAMGRTSKSCGQQYADRIIDIDILL
                     YDHLVIDQPTLKIPHPAIAERDFVFIPLLEIAPDLVDPLSGKKFSELVEAKKITSELK
                     NFVVGKKEEKFR"
     gene            complement(5148..5762)
                     /locus_tag="PLZ54_05495"
     CDS             complement(5148..5762)
                     /locus_tag="PLZ54_05495"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOJ66415.1"
                     /translation="MNQYVLYLACLVAGYLIGSVLFAQFITKLVSNQNIRELGNGNPG
                     AYNVFRNVSKFWGVLTGLLDAVKALVPMWIAHHFLHINDTTALGCIGIGAVIGHGLPL
                     YYHFKGGRAASTLLGMYAYFIGIELLIALAVDAIVVFGFIKKEYGIWGPSLLIALSGL
                     LCLFFPHELGVKILVWIGALITLFFNRDKLMEKKGKVTEEVSQQ"
     gene            complement(5775..6659)
                     /locus_tag="PLZ54_05500"
     CDS             complement(5775..6659)
                     /locus_tag="PLZ54_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOJ66416.1"
                     /translation="MSKKIFIIVGDNLGLSRKQIDYDALEIVKFPVFVDGVEYRQSEE
                     HNAKWLISKYEKEKVVAKSSTLIHNEIAEIIERNHANYDVFIHVVMSSGLSAASFRVA
                     ENVRKQFENIVPVINVDTHQTINGVGSVLLGIIDLIKTHPDLSVEELTAACQQIVENT
                     FSWFVFPDLNYLYKGGRIGKAQSLMGSVLHIIPVVGMMGNEKDGLILAMGKGRTYRQV
                     NDLIIDQITAKMQEKSVNRIKRAITLSGYGDKNQDAYAALLEKIKTLPVDDIVEGEGG
                     LADAVYCGPGAYGVSILL"
     gene            complement(6671..8512)
                     /locus_tag="PLZ54_05505"
     CDS             complement(6671..8512)
                     /locus_tag="PLZ54_05505"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOJ66417.1"
                     /translation="MNTTDTNLVETTEVSFNVESINEIIENIHRYLNKHKEYINSLNV
                     FPVPDGDTGLNMVLTIQGAIVQMKNNGRTEKNSGEYLKDFAEQMLLNSRGCSGVILSL
                     FAQGFTQITANNDFSKENIYRAIENGYRNAYEGTENPREGTMLTIMRALKEKYAQLME
                     KEENPLVIIQQTIPYLKEVLDQTPEMLPVLKKAGVVDSGGAGFIILLEGIDKELSQLA
                     VNGISLSSLLRIGRTTRKLLRKRLSELRKSPFAPLILNIDFSRIQNSRLVETLQSARN
                     LLGNIHLNHNNGKTLNREKLIDELQEIDDSWNPEIKNKYCTEFVLESDVITSKDQLKE
                     KIAHYGDSLIIINSNNKYKVHIHTNKPNDLFDDVSKFGSLLFTKVDDMKKQHRNFLSE
                     DVLDYERDKSVFCIVSGKGFADILKNLGADDILCYGKNKPSVNQLVKGLNNLKAKNII
                     AASDDSDILMALKYAASLCKSNVYIVESDNPISLVSMLMNISKDYDINTIFDEAMNSI
                     HNIPFCGIARSSRNVTTENGAPVNKNDYFTVYKKKIILANKNLDELLFDTVRQLSTEG
                     SLITLYKGADMKKENSFIERLRSKFPDRDFEEYFGGQYKYSYYITFE"
     gene            9038..9625
                     /locus_tag="PLZ54_05510"
     CDS             9038..9625
                     /locus_tag="PLZ54_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66418.1"
                     /translation="MKTIGCATTQHEIERLEQLAGEEMNRLSGNQTKLFGYESDERIE
                     QVFSLLHNGSIHPVGPELIFGKIYDYIGFNSIQEELFRHLVIAWLAFPLSKLKTADYL
                     RRYQGKEIEINTVYRFLGKLSGKLKPEVEQIDFAHTLEVLGGKINVVFYDLTTLYFET
                     SEEDDLRKMMGFSKEDKLLDVIKSVLFLQKIVVTN"
     gene            complement(9593..10444)
                     /locus_tag="PLZ54_05515"
     CDS             complement(9593..10444)
                     /locus_tag="PLZ54_05515"
                     /inference="COORDINATES: protein motif:HMM:NF016668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HOJ66419.1"
                     /translation="MKPHILIYNALFVFLAVSVNAQPKLIIRGDDAGSARSANLAIIQ
                     NYEKGILKTTEVMVPCPWFYEAAKLMQQHPGLDVGVHLTITSEWDGYKWKPLTHCPSI
                     CDSEGNFLANTWGGQSGNSLQYANINLTEVEAELRAQVETAIKLIPQISHISEHMVFG
                     SLSPELRNLVKKLASEYNLLFEDDLITQMGVRKMIVNQSEDYKMLRKNFIQSIDTLPA
                     EGIWIWIDHPAINGFETQGMKLADNVKNVANDRNNIYRLLNDRKVKRRISNKKIELIS
                     YDYFLKK"
     gene            complement(11582..12463)
                     /locus_tag="PLZ54_05520"
     CDS             complement(11582..12463)
                     /locus_tag="PLZ54_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOJ66420.1"
                     /translation="MDKRIKYIIEKIRFKDAIVVSEEDRKTSFAKIKERIAANEKVNI
                     PFEQKQTTFTLTFLQIAAAVAFIVSISALGFNIYFNQEINISNNTATIKNIVLPDGSQ
                     LELAKNTSVSYKRNFSYKRNIKLNGQALFSVSKNQQRPFTVQTKLGEIRALGTQFSVK
                     AFENNDFCKTYLIEGSVSVSNSINEILLKPGQEALITSNNSAITVIEVRNPQHAMAWK
                     TGIYIFEDESLTNILNTIADAHNKKLSINSLNAINKHYSVKFIRGEDLSTMLEVLADI
                     CPMRFRITQNEIIIDEK"
     gene            complement(12795..13349)
                     /locus_tag="PLZ54_05525"
     CDS             complement(12795..13349)
                     /locus_tag="PLZ54_05525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOJ66421.1"
                     /translation="MTTEDQILWKNIHEADDIEAFEELHARYFSALCNFTYSYLRNKQ
                     LCEEVVSDVFVALWQKRKELNHVRAIKSFMYTCAKNLSIDTIRKNKIRIQSDTSLYEI
                     EIPDADDHILSPLEQNEFKEHINIAIEQLPPQCKLIFKMLINDQLTYKEIAEVLNLSR
                     KTVEAQIAIAYKKITLHLKKIYHC"
BASE COUNT         4094 a         2586 c         2296 g         4441 t
ORIGIN      
        1 tggcatggcc cgtattgacg aagtggaatt caacggacaa aaaattgtgc cgattcagtt
       61 tttaaaagcc gtattaccca atcccggcga tttaggcgag aactacaaag gctggacttc
      121 cattggatgc cgcattcgtg gcataaaaga cggcaaagaa aaaacttact acatttacaa
      181 caattgcagc cacgaagcag cttacaaaga aaccggcacg cagggcgtaa gttataccac
      241 cggcgttcct gccatgattg gtgccatgat gtttttgcag ggaaaatgga aaaaaccggg
      301 cgttttcaat gtagaagagt ttgatccgga tcctttcatg gaacaactca acaaacaggg
      361 tttgccctgg cacgaattga taaatgttga tttggaactg taagttttta gaaggcaaaa
      421 taggaataat tatttttatc gaaaaaaaga gcgcgtaggg cgctcttttt tgttagttcc
      481 acaacaattc tgcatactga ttttcattga ttttttctgc tctgtaagca gctgcaaact
      541 gtaaaattct ttcaatcgtc tccatttgag gacacattac ttttgtcttt ttctgctgac
      601 cggagtccac ctcatcagca ctgttgtcgg aagtagtaac tttttgcata ggcatgtcta
      661 ttattagata caattttcat ctctcaaata ataaacgatg acacttgcca aaaaagtgta
      721 cggacaccca caaaaaataa taatattttt cccgcttatc tgaatttatt tgatttcgag
      781 agagatattt ttttctgaag ctatttttcg caagttgatc agcgcataac gcattcttcc
      841 caaagccgta ttaatgctta catttgtttt ttcggctatt tccttgaaac tcaaattttc
      901 gaaaaaacgc attttaacca cagcctgctg agaaagagga agtttttcga ccaaatgaac
      961 cacatcgaca aaaacctgtt cattagacaa cacatcttcc atactttttt ccgaaaaacg
     1021 tgtgcagttc aaaatatcaa agttaattcc ctctaccgaa aaggtgtttt cgttcttttc
     1081 gcgacgaaaa tagtcgataa ttaagttatg ggctattcga ttaatccatg ccaggaattt
     1141 tcccgattcg acataacgat cctgttgaat ggtagctatg gccttgataa aagtatcctg
     1201 aaaaatatct tcggtaagtt ccctgttgcg tacaatcaaa aaaatgtagg tatatacttt
     1261 cgactgataa cgtttcaata aaatctcaaa agcctgattg ttaccctctt tgtacaattt
     1321 taccaattca tcatcggtaa attgattgaa gtctttcatt attgctaatt tattggttaa
     1381 gggtaagaat cggcaatagg cattgaagat ttagaggttg actattaaaa aatttttagt
     1441 acgcaaaaat aataattgta ttgatataaa aaaatttttt acaacaaaat ttttcaaaaa
     1501 acgttacaaa ttttgagtaa ttctattcta tttccctatt ctacccacaa aaccaagcct
     1561 ttcagatatt ctccttccgg atgataaata ttgatgggat gatctgttcc ctgcgaaagt
     1621 tgatgcaaaa tacgcacatt tcgtttgctt tcggcagctg cactgaacac tacctgacga
     1681 aactgttccc gtgtaatggc ttgtgaacag gaaaaagtaa aaattattcc tccgggccga
     1741 atgatttcaa aagcttttct gttgagcttt ctgtaacctt tcaatgcatt cgggacggca
     1801 tccctgtgtt tggcaaaagc cggaggatca agaataatta aatcgtaaaa aggtgcacgt
     1861 ttttcgtaaa gttgcaaata tttaaaagcg tcttcggcaa aagattgatg acgattatcg
     1921 gaaaaattga gcgctacatt tttatcggtc aattctatag ctttcgccga actatcgact
     1981 gaatgaacca atttggcatt tccacgcaaa gcataaacgg aaaaaccgcc tgtgtagcaa
     2041 aacatattta atacaattcg atcggaagca taacgttcca gcaaaacgcg gttttcacgt
     2101 tgatcgagaa aaaaacccgt tttttggcca tgttgccagt cgacaaaaaa tttcagtccg
     2161 ttttccatga ccacaaactg ttcggaatct cctccaatta agtagccgtc ttcaggagca
     2221 ttttcattac gaaaaggcag ggttgcttcc gatttgtaat aaagatattg gatttcaggg
     2281 acgcattcta ccacacattt cgctaacaca ttcctgattt tgtgcatacc caccgagtga
     2341 gcctgcatga cagccgtatt gccatataca tctacaatca atcccggcaa accatctcct
     2401 tctccatgaa caagtcggta gcagttgttc cggttttcga tcatcaaacc cagagctcta
     2461 cgcatttgat aagcttccgc tattcggttt ttccagaagt tttcgtcaat tggctcttcc
     2521 tgaaacgaaa gaagacgaac cataatgctg ccctgctgaa aatggcctat tcctaaaaaa
     2581 ttcccttgac tgtcgagtac ttttaccaaa tcgccttccg aaggctgacc ttcaattttt
     2641 tgaatagcac cggaaaatac ccacggatga aatcggcgga cactttcttc ttttttgggt
     2701 ttcagaaaaa caacatgcat attaaatgtt ggattttaaa aattgaataa ttatcattcg
     2761 acaaaacaaa ctcaggaaag cttttctgaa tgagcatcgg gcttaagatc cgaatttgtg
     2821 gtttcggcat tttcggatgt ttcgggttgc ttgtgaggcg tgttttcctg caccagtttc
     2881 tcaatttgtt tgggggtcaa ttgtggctca ttcatcagtt gcagataaat ctgtaaactt
     2941 gcccaagcgt cggttgcggc atagcgaagc tgtttttccg tcaattgcgg tttttcccaa
     3001 tcgctgagac gttgagactt cgatattttt ttcccgaaaa taatggcgta aattttctgc
     3061 aaactcaact ccataatacc gtaactcttt gctatatttt gaatatctac aaaattagcc
     3121 ggatgaaaat tctgacgctt gttcagcaaa cgaaaatcat cgtgcaatga caagccgact
     3181 ttcttgatgt tttcatcatt taaaaaatcc tgcaattcct gtggaaaatc gattaaattt
     3241 agacgaaaaa ggaaacaacg ttccaactcg gaaagttgaa gcagggaaac tttattggaa
     3301 gtaccgcttt tgaaagaagg ttttgtttcg gtgtcgatcc ccacaacgag gttttttcga
     3361 agttcgtcga tagcgggcgc caccatttca ggtttatcca caagaacaat ttctcccgga
     3421 aaactgacta cgggtaactg attgatttcc tctttcgaaa tttttggtga atacataaaa
     3481 aatttatttc aaaaaaggac ttacatcgtc gttctcggca gtatgttgaa aatcgagaaa
     3541 cgaatcgcgc gtaagttgtt tttcatcagg ttgatgcatt tcttcccatt ctgtatcatc
     3601 ctgcgggttg tatttcaaag catgtaaggc acgcatggca ttcagcaact tttgacccca
     3661 atgttcgcca aaagccgtaa gacattcaga tagagcatcg ctcattatgt ccacatcagc
     3721 agtttggtaa ttggcagcaa aatccttgat ttcctgataa atatcactca tattttcaga
     3781 aataaaagct gcaacaggtg tatcgcttat ggccatgtcg ggatgaaaca cctcaaggta
     3841 cacatcatcg ttgcccaaaa gttgttcgat ttgttcacga acaaactgat aatcttcctc
     3901 agtaacaaaa cgttcaggaa gattttcata aaccggttcg ggaatatcga gcacggaagc
     3961 cttcagataa agcaacggca atagtttcag cgatttttcc acaaactcat ccttccccat
     4021 ttccgaagta tgttccagaa aaaggcaggt ttcggcaact actgttacaa attcaataac
     4081 attggcgctg taaacgatat gatcgggcaa ttcgtcaata ctcatgataa tttcaataaa
     4141 acttattcat tgtagcaatg aaaattttta aagtacaaaa ttaagaaaat tattttacat
     4201 aaaccggtga aatctttcag gccccatctc aaaaaaagat agtcaacttt cgaaaaattt
     4261 gataaacaat ttttatcttt accttgttct aaaccaaaaa ggacatagca aaagcataat
     4321 ttattttcta ccaatacccc aaaacaaagc agtatggaag acaaaattgt aacactaaaa
     4381 aattacgaca caatagtgaa agccatgttc gatcaggaaa ttttacgtgc caaccacatc
     4441 gaatgcttta tcaacaacga gcagttggtg gagctttatc caatgtttgc cgacattgat
     4501 gaaggtttga aaatagttgt ttttgaaaaa gatttggaaa aagcacagaa ggtattggaa
     4561 gaataccata gctcaatcga agaaaatgtt taacgaaatt tctcttcctt ttttccgaca
     4621 acgaaatttt ttaattcgct tgttattttt tttgcttcaa ccaattctga aaactttttt
     4681 cctgagagcg gatcaacgag atcgggagca atttccagca agggaatgaa tacaaagtcg
     4741 cgctccgcaa ttgcgggatg cggaattttt aaagtaggtt ggtcgatgac aagatgatca
     4801 taaagcaata tgtcaatatc aataatacgg tcagcatatt gttgcccgca actttttgaa
     4861 gttctaccca tcgcctgctc aatttcccgg gtcttgtcca acaattcgca gggagataaa
     4921 tgggtttcca tcagcactac cgcattcaaa aaatcgtttt ctgaagaaaa accaaatggt
     4981 ttggaaatat aaaacgaaga ttgggacagt aaactcccaa tctccatttt taacatcatc
     5041 acagcatcct gcaaattttt ttctttttct ccaagattgc ttcccaaacc caaaaaaatc
     5101 agactcattt ttaaaaagat tactttcttt gcatttctca ccaatgatta ttgctgcgaa
     5161 acttcctccg taacttttcc ctttttctcc atcagcttgt cccgattaaa gaacaaggta
     5221 ataagtgcgc caatccaaac cagaattttg acacccaatt catgaggaaa aaacaggcaa
     5281 agtagtccac tcagtgctat caggagggaa ggtccccaaa taccgtattc ttttttgata
     5341 aaaccaaaca ccacaatagc atctaccgct aaggcaataa gcaattcgat gccaataaaa
     5401 taggcataca ttcccagcaa cgttgaagcg gctcttcctc ctttaaaatg gtaataaagc
     5461 ggaagaccgt gcccaattac ggcaccaatt cctatacatc ccaaagctgt tgtatcgtta
     5521 atatgcaaga agtgatgagc tatccacatg ggcacaagcg ctttcacggc atccagcaat
     5581 cctgtgagta cgccccaaaa tttgctaaca tttctgaaaa cattatacgc tcccggattt
     5641 ccgttgccca attctcgaat attttgattg gaaaccagtt tggtaataaa ctgggcaaat
     5701 aataccgaac caattaaata tcccgcaaca agacaagcca aatataaaac atactgattc
     5761 ataatatgta aactttacag aagaatagaa actccataag cgcccggtcc acaataaaca
     5821 gcatctgcca gtccgccttc accttcaaca atatcatcaa ccggcagcgt tttaattttt
     5881 tccagcaggg ctgcataagc atcctgattc ttatctccat aaccactcaa cgtaatggcg
     5941 cgtttgatgc gatttacaga tttttcctgc attttggcgg taatttgatc tataatcaaa
     6001 tcgtttactt gtcggtacgt tctgcctttc cccatggcca aaatcaatcc atctttttca
     6061 tttcccatca taccgaccac cggaataatg tgcagaacac ttcccatcaa cgactgcgct
     6121 tttccgatgc gcccgccttt atacaaataa ttgagatcgg gaaaaacaaa ccagctgaaa
     6181 gtattctcca caatttgttg acaagctgcc gtcagttctt ccacacttaa atcgggatgg
     6241 gtttttatca agtcaattat tcccagtaat acgctgccga cgccattgat ggtctgatgg
     6301 gtatcaacat ttataaccgg cacaatattt tcgaactgtt tgcgaacatt ttctgccacg
     6361 cgaaatgaag ctgcagaaag cccactgctc atcaccacat gaataaacac atcgtaattg
     6421 gcatgatttc tctcaatgat ttccgcaatt tcattgtgaa tcaaagtgga agattttgcc
     6481 acaacttttt ccttttcata tttcgaaatc agccattttg cattatgttc ctcactttgc
     6541 cgatattcca caccatccac aaaaactggg aattttacaa tttccaacgc atcatagtca
     6601 atttgtttgc gtgaaagtcc gagattgtct ccgacaatga taaaaatttt cttgctcata
     6661 gtttcaaatt ttattcaaag gttatataat aagaatattt gtattgtcca ccaaaatact
     6721 cttcaaaatc ccgatcggga aatttactcc tcaaacgttc aataaaagaa ttttccttct
     6781 tcatatctgc gcctttgtac agggtaatta aactcccttc cgttgaaagt tgccgaacag
     6841 tatcgaaaag aagttcatcg agatttttgt tggcaagaat aattttcttt ttatacaccg
     6901 taaaataatc gtttttattc accggagcac cattttctgt tgtaacattt cgtgaagagc
     6961 gtgcaatgcc acaaaaagga atattgtgaa tactgttcat ggcttcatca aaaatggtgt
     7021 tgatgtcgta atcttttgag atattcatca acatactgac caacgaaatc ggattatccg
     7081 attcaacaat gtaaacattc gacttgcaaa gcgacgcggc atatttcaat gccatcagaa
     7141 tatcgctgtc gtctgaagct gcaataatat tttttgcctt caaattgttc aatcctttca
     7201 ccagctgatt gacggaaggt ttgtttttgc cataacacag tatgtcatca gcgcccaagt
     7261 ttttcaggat atcagcaaaa cctttccctg aaacaataca gaatacgctt ttgtctcttt
     7321 cgtaatccaa cacatcttcg ctaaggaaat ttcgatgctg ctttttcata tcgtccactt
     7381 tggtaaaaag caaagaacca aatttggaaa catcatcaaa caaatcattg ggtttattgg
     7441 tgtgaatatg tactttgtat ttattgttgc tgttgataat aatcagactg tcgccgtaat
     7501 gagcaatttt ttccttcagt tgatctttag aagtaattac atcactttcg agcacaaatt
     7561 cggtacagta tttatttttt atttcaggat tccatgaatc atcaatttcc tgaagctcat
     7621 cgattaattt ttccctgttc agggtttttc cgttgttgtg attcaaatga atattcccta
     7681 ataaatttct ggcgctttgt agcgtttcaa caagtcggct attctggata cgggaaaaat
     7741 ctatattcaa aatcagtgga gcaaatggac tttttcgtaa ttccgacaat ctctttctta
     7801 gtagttttcg cgttgtgcgt ccaattctaa gcaagctgga taaagaaatg ccgtttaccg
     7861 ccagttgact gagttcctta tcgattcctt ccaacaaaat aataaaaccg gcgccccccg
     7921 aatcgaccac tccggctttt ttcagtacgg gaagcatttc aggcgtttga tccaacactt
     7981 ctttcaaata aggaatggtc tgctgaatga ttacaagcgg attttcttct ttttccatga
     8041 gttgagcata tttttctttt aaagccctca tgatggtaag catggtacct tctcgaggat
     8101 tctccgtgcc ttcgtaagca ttgcggtaac cattttcaat agcccggtaa atattttctt
     8161 tggaaaaatc gttgttggca gtaatttggg taaatccctg agcaaataac gataaaataa
     8221 cgcctgaaca accacgactg ttgagcagca tttgctcggc aaaatctttc aaatattctc
     8281 cggaattttt ctctgttctg ccattatttt tcatttggac aatagcaccc tgaatagtta
     8341 gcaccatatt caatcccgtg tctccgtcgg gaactgggaa aacgttcaaa ctgttaatat
     8401 actccttgtg cttattaagg taacgatgaa tattttcaat aatttcattg atggattcca
     8461 cattgaagct tacttcggtg gtttcaacta aattggtatc ggttgtattc atgatgattt
     8521 aaataattta cccagatttt taaaaatact ttggtttaaa atatgagttc caaaagtata
     8581 aaattttatt taaaaaacat tcagaaaaca aacaacttaa tctattgatt atttaaataa
     8641 aagattttca ataaaatatg caaactcgga cttaaaaaac ggtaaaataa ctttttgtgg
     8701 ttttttctca gcaaaaagaa tactaacagt attttcctta cccgaaacaa ggcaacaatt
     8761 tcagtgttac tgcatttttg tcagggaaaa aggtgctaaa atcggctcgc ctgtcaaata
     8821 tgcgttttaa aagtaagaaa aaatatcttg gtaaaaaata cttttcctgt tttccgcatt
     8881 ttttgcaaca tccttataag tatttgaata tcaataataa taatatttta attttgggtg
     8941 ttgcaaaatt attattatgt ttatccgaaa agtaaaaaat cgatctggaa gtcaatctgt
     9001 acaagtgata cagaagtttc gaggaaagta taaagttgtg aaaactattg gttgtgccac
     9061 cacgcagcac gaaattgaaa ggttggaaca attggccgga gaagaaatga atcgtttaag
     9121 cggaaatcaa acgaagcttt ttggctacga aagtgatgag aggattgaac aagttttttc
     9181 tctgcttcac aatggcagta ttcatcctgt tggtccggag ttgattttcg gcaagattta
     9241 cgattatatt ggttttaatt ccattcaaga ggagcttttc aggcatttgg ttatagcctg
     9301 gttagcgttt ccattaagca agttaaaaac agctgattac cttcgtcgct atcaagggaa
     9361 agaaatagag ataaacacgg tttataggtt tttgggcaaa ttaagcggga aactcaaacc
     9421 agaagtggaa caaattgatt ttgcccatac ccttgaagtt ttaggcggca aaattaatgt
     9481 tgtgttctat gacctgacta cactgtattt cgaaaccagt gaggaggatg atttgcgcaa
     9541 gatgatgggt ttcagtaaag aggataaatt gctggatgta atcaaatcag tattatttct
     9601 tcaaaaaata gtcgtaacta attaattcaa tttttttgtt ggaaatcctg cgtttaactt
     9661 tcctgtcgtt caacaatcta tagatattat ttctgtcgtt agcaacattt tttacattat
     9721 ctgcaagctt cattccctgc gtttcgaaac catttatggc aggatggtca atccatatcc
     9781 atattccttc ggcagggaga gtatcaatag actgtataaa gttttttctc aacattttgt
     9841 agtcttcact ttgatttaca atcatttttc ggacacccat ttgggttatc agatcatctt
     9901 caaaaagcag gttgtattcg gatgcaagct ttttaaccag atttcgcaac tcaggtgata
     9961 aacttccaaa aaccatgtgt tccgaaatgt gacttatttg tggaattagt ttgattgctg
    10021 tttcaacttg tgctcttaac tcagcttcca cttcggtaag atttatgtta gcatattgaa
    10081 gcgaatttcc ggattgacca ccccaggtat tcgccaaaaa gtttccttcg ctatcgcaaa
    10141 ttgagggaca gtgagtcaga ggtttccatt tataaccatc ccattcgctt gtaattgtca
    10201 gatgaacacc cacatctagc ccgggatgtt gttgcataag ctttgctgcc tcataaaacc
    10261 agggacaagg caccatcacc tctgttgttt ttaaaatccc tttttcgtag ttttggatga
    10321 ttgccagatt tgccgaacgt gcggaacccg catcgtcgcc tcgtataatt aattttggtt
    10381 gagcatttac actaacggct aaaaagacaa atagtgcatt gtagataaga atatgtggtt
    10441 tcatcagaat ataatttatt ttttaaaggt ctgatggaac cccaatgtct gaatattata
    10501 tttattcgtg tttgtgtatg caatacatgt gggtttcata tcagaattga tttttctatt
    10561 gcaaatgtac gcgataaact gcaaacaatt atgtatttat ttatcattta ttaatactat
    10621 ttcgatgtct cataccaagt tgatatctaa aatatttaga taaggaaaca aagtcataga
    10681 tttcacataa ttaggtttgc atttaattcg tttattcccc ggcaacccac ttccattgtt
    10741 ttcgctaaag aatcaaatat cttgttgtgt aaatacttta ctcttatttc atcccaaaga
    10801 tgttgtgttg aattaacttc aagactaaaa gatggttgct atatgaaatg tatatttccg
    10861 ggcactctta aaccttttgc tttgtgccat cctgttccat ctatctgcat gataatttca
    10921 aattccttaa attcattgga tatattttat aaaaataagc tcatcatttc cgtatttgca
    10981 tgcggtaata ttaaagaact aatgtggcct aactttggac atattcctgt ataaacataa
    11041 aaaaactttc tcttactact tattttgcaa ccagaggacg aacctgcttg ggtgctcaac
    11101 aactcttgaa atatttactc tttaaatctt cattcatcct gtgtcatgag aacgacttta
    11161 cttttatcaa gatttttctc ttcaatcaat tgactcactt tattaccgaa gtttgttaaa
    11221 agcttcttct tcctccttgt tctgtttggg gtgtacagga agggggtaca atttttctcc
    11281 aaccatttct tttaagcatc ctgtaaacag tagttttgtt aactctcttt cctgtcttct
    11341 tttcaaaatt ttctttgatt tacatggcag ttattatttg tccttctctg gctttctcca
    11401 aataactaca aataattcct cctcttcctt acttaagtat gaatgcctcc ttccaccttt
    11461 accttgagtt tctatagacg ctattccata ttcaatgaat ggaaagttgg gttaaatcag
    11521 ctgcttaatc taaaaaaaaa agtgtctttt taggacacct tcttttttta cttttaatta
    11581 actatttttc gtcgattatg atttcatttt gagtaattct aaatctcatt gggcatatat
    11641 cagccaagac ttcgagcatt gtggataaat cttcaccacg aatgaatttt actgaataat
    11701 gtttgttgat agcatttaag ctgttgattg atagtttttt gttgtgtgca tcagcaatag
    11761 tattaagtat gtttgtcaac gattcgtctt cgaaaatata aattccagtt ttccatgcca
    11821 tagcatgttg cggatttcta acttcaatta ctgttatggc gctattgtta cttgtaatca
    11881 aagcttcttg tcccggtttt aacaatattt cattaataga gttagacacg cttacacttc
    11941 cttcgatgag atatgtttta cagaaatcat tgttttcgaa tgctttaaca ctaaattgtg
    12001 tccctaatgc tcgtatttcg cctagttttg tttgaactgt aaatgggcgc tgttgatttt
    12061 tactaactga aaacaaagct tgcccgttga gcttgatatt acgtttatat gagaaattac
    12121 gtttataaga aaccgatgtg ttttttgcta attcaagttg tgatccatct ggtaaaacaa
    12181 tatttttaat ggttgccgtg ttgtttgaga tattaatttc ttgattgaaa taaatattaa
    12241 agcctaatgc cgaaattgaa acaataaatg ctacagcggc tgcaatttgt aaaaatgtaa
    12301 gtgtgaatgt tgtttgtttt tgctcgaatg ggatatttac tttttcatta gcagcaatgc
    12361 gctctttaat ttttgcgaat gatgtcttcc tatcttcttc tgagacgaca attgcgtctt
    12421 tgaaccgaat tttttcaatt atatatttaa ttcttttatc cattttttgt tattagctta
    12481 ataacactaa acttaatata ctttttaaaa actaaccctt ttccttgtag acgaaagatt
    12541 tttgtttttg ccttaaaatg taaaataaat cgtaaattcg actaaatcat gccacctttt
    12601 aagggaaaat aaaagatgaa gaaaaattat ttttcaagtc aaaggtgaaa aaagagtatt
    12661 taccctttga cctcatgcaa taaatgctta attttattct cttaccctta aaaagttaca
    12721 aagatgaaac atttaaccgt agaacaaagt tacaaatttg tctaaaatta aaaagttgca
    12781 tttctaactt gaatttagca atgatatatt ttttttaaat gtaaagtgat ttttttgtat
    12841 gctatagcta tttgagcttc aactgttttt cgtgataaat tgagcacttc agcaatttct
    12901 ttatatgtaa gttgatcgtt tattagcatt ttgaaaataa gtttacattg tggcggaagt
    12961 tgctcgatgg ctatatttat atgctcttta aactcatttt gttcaagtgg tgaaaggata
    13021 tggtcgtctg catctggtat ttcaatttcg tataaacttg tatctgattg aatgcgtatt
    13081 ttgtttttac gaatggtgtc aattgataga tttttagcac aagtatacat aaatgattta
    13141 atagccctga catgatttaa ttctttgcgt ttttgccaca gtgctacaaa tacatctgat
    13201 actacttctt cgcaaagttg tttgtttctt aaataggaat aggtgaagtt gcacaatgcc
    13261 gaaaaatacc tagcatgtag ctcttcaaaa gcttcaatgt catctgcctc gtgtatgttt
    13321 ttccacaata tttgatcttc agttgtcatt tgttgatttt atcccaaatt gtccattaga
    13381 acaatatgat attaggatta ttttttttag tcagtat
//
LOCUS       DAOSCH010000055        13298 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3156_length_13298_cov_6.198369, whole genome shotgun sequence.
ACCESSION   DAOSCH010000055 DAOSCH010000000
VERSION     DAOSCH010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13298
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3156_length_13298_cov_6.198369"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            352..3048
                     /locus_tag="PLZ54_05530"
     CDS             352..3048
                     /locus_tag="PLZ54_05530"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOJ66422.1"
                     /translation="MKRKNGRILGIIFSILIVLFAVLMILPFAFQGKIFNAVKLQINK
                     MLTAKVDFQNVQLSFIRHFPNASVRINGLKVIGEGDFAADTLVSADKIEAVVNLKSLF
                     SGKGYDIQKINVVNPHIMAHVLADGRANWNIMKDTAEEKTDTAAMKFHLKLKDVFIQH
                     ANIAYLDEQSNMKAIISNLNHHTSGDLTADSSLLSTQTTIDSISFWMNGIPYLLRANA
                     ELNVDIDANINDMKFAFSKNSSRINAIPFSFEGWVKMLDEGFDMDIKLIANKVDLKSI
                     LSMIPAIYSNSFKDLKTGGEVSLNGFVKGQMKDENYPAFDVNLSINDGWFQYPSLPQS
                     VKNIQLAFQIVNPGGSLDSTLIDLSKFSLTLGSNTFNAAAKIASIISDPDIRAKASGR
                     IDLGMVKSFYPLEPGTNLNGLLDLDLNLNGKMSYLENNLYDKFEFQGKLLANNIFLKT
                     SSLPQDISVNRAQVFFNNRYVNLSDFQLKIGKNDLQANGKLENFLAYALKNKTLKGQL
                     NLQSNYLNVNDFLTETTETKNEKMELLVIPQNIDFTLQAFFKQLLYDKMNFSNIQGKL
                     LVQNGEIKFQNLNLDAFGGTLLANGLYSTANVAKPYADINLNIKNVAFNQIFSQVASF
                     QKFVPFFEKATGNFSTTLSLNTLLNNDMSLNLSSLKANGKFSTSSVALKDVPVLSALA
                     SGLKKPELSNPSLKDLAVNFEISNGKLFTKPFDIKLGDIKMNLGGSMGLDQSLDFKGI
                     VQLPDQLNAGRFSNFNVTIGGTYDKPKVKLDLLDTFNEALEETKAKIKEEVTTQVDAA
                     KKEVVEKARKEREAALKEAQVQADKIRLEAKKAGDKLIEEATLKGNELVEKAANPIAK
                     KVAEQSARKLVEEARKQADKLNADAEIQANKIIENASKNTNF"
     gene            3124..4515
                     /locus_tag="PLZ54_05535"
     CDS             3124..4515
                     /locus_tag="PLZ54_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ66423.1"
                     /translation="MRSKISLITVLLFFCVAILGQTAAEADRLFQQNQFQKARDAYKI
                     LLEKRPKDALYNYRYARCCYELKDDENAIKHFELSGNRFPLTNKYLGELYMKTYRFSQ
                     SVAAYEEYLKTINETDKEFLTIQKAKKKAELAEKMIQRVDDIQIIDSVVVNKDNFLEF
                     YKFTNDLGSLSATPVKIGKKKDFKITYTTQRQDRKLFSDTVHGQMDIFTSYKLLDEWS
                     KPESVSDIINTKANENYPFLLLDGVTIYFASDGDNSIGGYDLFVSRFRPETNTYFQPE
                     NIGMPYNSIYNDYMMVIDDIHQVGWFATDRFQPEGKVIIFSFIPNTKKTILQSEDSEY
                     IRKAAQLQLFKKAKNFNSDIIPQTSFADEDSVHKIFFVVNDSVIYTDDNQFKTEETRN
                     LWNELEKLNSQLNQKRIQLEQLRMQYSQQEETQNELAPKILTLEKEISEMEDQLTTKT
                     FELRNKENINLMK"
     gene            4563..5054
                     /locus_tag="PLZ54_05540"
     CDS             4563..5054
                     /locus_tag="PLZ54_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOJ66424.1"
                     /translation="MDIAIAIILILVGVFFFLVEFFLVPGISFAGIAGFLFVGAGIYY
                     SYSQLGTTAGNVSIAGSVVLLAVAVWIFLRRKTLERISLKAKIDSSIGSVEELNIHAG
                     DTGIALSRLAPMGKVKVNGQTIEAKSVDEFIDEGTPILVKEVFKTNVLVERISENSNN
                     EHA"
     gene            5069..6037
                     /gene="floA"
                     /locus_tag="PLZ54_05545"
     CDS             5069..6037
                     /gene="floA"
                     /locus_tag="PLZ54_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213154.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOJ66425.1"
                     /translation="MGIEIVLLIVLVVVLLLFFYYVPFLLWLSAKVSGVSISLVQLFL
                     MRIRKVPPHSIVQCLIEAHKADLHEIKRDELEAHYLAGGHIERVVHALVSASKANIDL
                     TFKMATAIDLAGRDVFQAVQMSVNPKVIDTPPVAAVAKDGIQLIAKARVTVRANIKQL
                     VGGAGEETILARVGEGIVSSIGSAESHKAVLENPDSISKLVLKKGLDAGTAFEILSID
                     IADIDIGKNIGAQLQMDQAEADKNIAQAKAEERRAMAVALEQEMRAKSQEMRAKVIEA
                     EAEVPKAMAEALRNGNLGVMDYYRLKNIEADTAMRENIGKPSDKTK"
     gene            complement(6236..7243)
                     /gene="murB"
                     /locus_tag="PLZ54_05550"
     CDS             complement(6236..7243)
                     /gene="murB"
                     /locus_tag="PLZ54_05550"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOJ66426.1"
                     /translation="MNIKYNYSLLSHNTFGIDVKAEEFIEFYTVEEALDLSKTLKQKK
                     WFVIGQGSNLLFLNDFKGVVLHSGIRQMKKIKEDDQFVYFEVGSGVVWDQFVEFCVKN
                     NWSGIENLSSIPGEVGAAAVQNIGAYGMEVQEAIEKVKAIDTATSQLMEFSKENCQYG
                     YRQSIFKNQYKGRYILTSVVFRLSKKPNFRLHYQHLEEEVRKRGNINLQTIRETIIQI
                     RSQKLPDFRTMGNAGSFFVNPVVSKVHFQRLQSEYPEIPHYYVSETEEKIPAAWLIEQ
                     CGWKGKQVGKVAVYAKQPLVIVNCGGASGIEIKQLAEEIQLSVKNKFDIQLQPEVNYI
                     E"
     gene            complement(7254..8156)
                     /gene="miaA"
                     /locus_tag="PLZ54_05555"
     CDS             complement(7254..8156)
                     /gene="miaA"
                     /locus_tag="PLZ54_05555"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOJ66427.1"
                     /translation="MQSTLIVILGPTGVGKTEISLQVAQNLQTPIISADSRQIYKELK
                     IGTAAPTSEQLSTVKHYFIGSHSIFENFSAGQFEQEALALLEKHFATNHIALMVGGSM
                     LYIDAVCKGLDNIPTVDTTTRNFWKKQYENQGLEYIRQELKKLDPVYYQQVDPNNYKR
                     ILHALEICSITGNPFSALRTGVHKKRAFDIVKIGLNRPRHELYERINARVDQMMEEGL
                     LEEAKQFYAFKNLNPLNTVGYKELFEFMDGKITLKQAVDKIKQNTRHYAKRQLTWFNH
                     DKDITWFHPDEREKIIRFLQNRLS"
     gene            complement(8223..8783)
                     /locus_tag="PLZ54_05560"
     CDS             complement(8223..8783)
                     /locus_tag="PLZ54_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66428.1"
                     /translation="MVFRFTILSDEVDNFLRVIDIDAEATFFDLHNAILDAVNFKKDQ
                     ITSFFLCSDDWEKEQEITLIEMDSSSEYDNLVMDKVKLEELLTDEKQKLMYVFDMIAD
                     RAFFIELSEIIPRKKLEKPVCIKSIGNPPEQTLSENDVNQAPKLNVEENFYGDEDFEL
                     DELDEEGFSDFNFDDDSNLFNDDTNF"
     gene            complement(8930..10312)
                     /gene="dnaA"
                     /locus_tag="PLZ54_05565"
     CDS             complement(8930..10312)
                     /gene="dnaA"
                     /locus_tag="PLZ54_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOJ66429.1"
                     /translation="MHEQLWERCLKVIRDNINEASYKTWFEPIIPLQYENNRFVVQVP
                     SQFFVEYIEEKYIDLLSRTIYREIGAGTQLEYRVLIDQTNGKGTQIPSTAEQPKLKNS
                     NATQGSKNPYDKSSLPNIDPQLNMKYNFNTLIEGKSNKLARTAGITIGAEPGKTAFNP
                     LFVYGESGVGKTHLANAIGVKAKQMHPDKRVLYLSANTFQIQYTDAVRNNTVNDFVNF
                     YQTIEVLIIDDIQEFSGKTATQNTFFHIFNHLHQSGKQLILTSDRSPLLMAGLEQRLL
                     TRFKWGLSAEIEKPDFELRKEILKNKIYCDGLDIPEDVVNFIAQNVTDNIRDLEGVLI
                     SLLAHSTLTDMPIDLQLAEKVVSRIVNIPKKINTVEKIRDTVCDYFSIPVDAIFTKSR
                     KREVVQARQIAMYLSKQFTKNSLSSIGKAIGERDHATVLHACKIISDLMDTDKKFRSN
                     VKAIEEILRN"
     gene            complement(10723..11634)
                     /locus_tag="PLZ54_05570"
     CDS             complement(10723..11634)
                     /locus_tag="PLZ54_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOJ66430.1"
                     /translation="MKKNFFNKEVKIGLMVFVAIFLLYFGLNFLKGIDIFTSTRSYYT
                     TYENLDGLVASSPVFIKGYKVGQVDEIQYDFSKEKPFLVKITIEKKINLPKNTKIQLF
                     DNGLMGGKAIQLIFAPYASSQNFCNNGDTLQSEIKPGLMADLSSNLLPKIESISTQTD
                     SLLRSIRSITEGEKLNNSLTSIEKTTADLAETSAQMKNLMKYQLPSIMSNLNGASSDF
                     KSVTSNLKEINFASTMNNVDQTVSNLNLLTNKINNGEGSLGMLLTDQQFYLNLANTSS
                     SANQLLIDLKNNPKRYVHFSLFGSKTK"
     gene            complement(11752..12978)
                     /locus_tag="PLZ54_05575"
     CDS             complement(11752..12978)
                     /locus_tag="PLZ54_05575"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ66431.1"
                     /translation="MKIPFQKYLLLPYGFLILFLSIFASSVAAQEKKFVVVIDAGHGG
                     HDPGAMGKISREKDINLAVAKELAKLMENNKELKVVLTRKTDKYLTLQERADIANENH
                     ADLFICIHTNSSKSSSAYGAETYTLGLAKTNANLEVAMRENSVILLEENYQTKYQGFD
                     PTSVDSYIMFEFMQDKYLDKSIEFASLVQQHLDRNGRLDRGVRQAGFWVLHRSACPST
                     LIELGFISNLEEELFLSSEYGQKKMAQAIYTAFLEFKKEHDKRNGKPNSTSVSNQAPS
                     KKSENIVSESQNNYSTPIQQAEIENINTANITNQEKQTENILPSTQQPVFKLQILASK
                     KKLSPQANELKGLKSVDYYIENELYKYTVGAETDYNKIVQLKNNLSAKFPQAFIVAFV
                     NGKKVDVKEALRLTGK"
BASE COUNT         4136 a         2276 c         2478 g         4408 t
ORIGIN      
        1 cattttttat gatggccaaa ataactgctc gtcgctccag ccctgacctt cttttaaaat
       61 catcatcccc tccaaaaccc ttctaacata gtgaaatata agaatatatg caaatttttc
      121 aaaaaacttt ttgaatattt tgtatccttt tttatcttaa tgtctttaaa aatctaattc
      181 tctttgagta actttttgga ggggactcat gattgagaac ttttcatttt cagtgcaaag
      241 ttagaggttt tccggaataa gagaaaaata attgaaaaac caaaaaaaag ttttactttt
      301 ttcggcagtt tattgtacct ttgttttgat tcagtttaaa aattttgcaa tatgaaaagg
      361 aaaaatggac gaattcttgg aataattttt tcaatcctca ttgttttgtt tgctgtcctg
      421 atgattttac catttgcttt tcaggggaaa attttcaatg cggtgaaatt gcaaatcaat
      481 aaaatgttga cggcaaaagt tgattttcaa aatgttcaat tgagttttat tcgacatttt
      541 ccaaatgctt cggtgaggat taacggatta aaagttatcg gagaaggtga ttttgctgct
      601 gacacgctgg tttctgccga taaaattgag gcagttgtaa atttgaaaag tttgttttcc
      661 ggaaaaggtt acgatattca aaagatcaat gtagttaatc cgcatataat ggctcatgta
      721 cttgctgatg gaagagctaa ttggaacatt atgaaagata cggcagaaga aaaaaccgat
      781 actgctgcca tgaaatttca tttaaaatta aaggatgttt ttattcaaca tgccaatatc
      841 gcttatttgg acgaacagag caacatgaaa gccattatca gtaatctgaa tcatcacacc
      901 agtggcgatt taactgccga cagttcgctt ctgtctacac agacaaccat cgattccatt
      961 agcttttgga tgaatggtat tccttacctt ttaagggcca atgcagaact gaatgtagat
     1021 atcgacgcca atattaacga catgaaattt gcttttagca aaaattcttc acgaatcaac
     1081 gctattccat tttcttttga agggtgggta aaaatgttgg acgaaggttt cgatatggac
     1141 attaagttaa ttgccaataa agtagattta aaatcaattc tttccatgat tccggccatc
     1201 tattccaatt cttttaaaga tttgaaaacc ggtggagaag tcagtttgaa tggttttgtc
     1261 aaaggtcaga tgaaagacga aaattatccg gcttttgatg tcaatttgtc gataaacgat
     1321 ggctggtttc aatatccttc attgcctcaa tccgttaaaa atattcagct tgccttccaa
     1381 attgtcaatc ccggtggttc gctcgacagt actttaattg acctttccaa gttttcttta
     1441 acgttgggga gtaatacttt caatgctgca gctaaaattg cttccatcat ttccgatccc
     1501 gatattcgcg ccaaagcttc agggcgaatt gatttgggaa tggtaaaatc cttctatcct
     1561 ttggaacccg gcacgaattt gaacggcttg ctcgacctcg atttgaattt gaatgggaaa
     1621 atgtcgtatc tggaaaataa tttgtacgat aaatttgaat ttcagggaaa attgctcgcc
     1681 aacaatattt ttctgaaaac aagctctttg ccacaagata tttctgttaa tcgagctcag
     1741 gtttttttca ataaccgata tgtaaattta tcggactttc aattgaaaat cggcaagaac
     1801 gatttgcagg caaacggaaa acttgaaaat ttcctggcct atgctttgaa gaacaaaact
     1861 ttaaaaggac agctgaactt gcaatccaat tatttgaacg tgaatgattt cctcaccgaa
     1921 acaacagaaa ctaaaaatga aaaaatggaa ttgttggtaa ttcctcaaaa tattgatttc
     1981 acacttcagg catttttcaa acaattgctt tacgacaaaa tgaatttttc caatattcag
     2041 ggaaaacttt tggtacaaaa tggtgaaatt aaatttcaaa atctcaattt ggatgccttt
     2101 ggaggaacgt tattggcaaa tggactttac agcacggcaa atgttgcgaa accatatgca
     2161 gatataaatc tgaatattaa aaatgtggct tttaatcaaa ttttttcgca agttgcttca
     2221 tttcaaaaat ttgttccatt tttcgaaaaa gctaccggaa atttttctac cacattgtcg
     2281 ttgaatactt tattgaataa cgacatgagc ttgaatctga gttcattaaa agccaacgga
     2341 aaattttcaa cttcgtcagt tgctttaaaa gatgttccgg tattgtcggc attggcatcg
     2401 ggcctgaaaa aaccagaatt aagcaatcca tcattgaaag accttgctgt gaatttcgaa
     2461 atatccaacg ggaaattgtt taccaaacca ttcgacataa aattgggcga tattaaaatg
     2521 aatttgggag gttcaatggg tttggatcaa tcgcttgatt ttaaaggaat tgttcaatta
     2581 ccggatcaac tcaatgccgg tcgcttttca aactttaacg taacaattgg tggaacctac
     2641 gataaaccaa aagtgaaatt ggatttgttg gatacattta acgaagcgct tgaggaaaca
     2701 aaagcaaaaa ttaaagaaga agttaccaca caagtagatg cagccaaaaa agaagtcgtc
     2761 gaaaaggctc ggaaagaacg tgaggctgct ttaaaagaag cacaggttca agcagataaa
     2821 ataagattgg aagctaaaaa agctggcgat aaactgattg aagaggccac cctgaaaggc
     2881 aacgaattgg ttgagaaagc agccaatcca attgccaaaa aagttgccga acaatccgct
     2941 cgtaagctgg tagaagaagc acgcaagcaa gctgataaac tgaatgcaga tgccgaaatt
     3001 caagccaata aaattattga aaatgcatcg aaaaatacga atttctaatt ctaaagcatt
     3061 tatgcaagat tgttaattaa gtaccttaat ttcaacaaat tatatatttt gaaaatattt
     3121 gtcatgcgtt caaaaatttc tttgattaca gtattgttgt ttttctgtgt agcaattttg
     3181 ggtcaaactg ctgccgaagc agaccgactt tttcagcaaa accagtttca gaaagcacgc
     3241 gatgcctata aaatattatt ggagaaaagg ccaaaggatg ctttgtataa ttatcggtat
     3301 gcgcgttgct gttacgaatt gaaagatgat gaaaatgcca taaaacattt tgaactttca
     3361 ggtaatcgat ttcctttgac aaacaagtat ttgggagaat tgtatatgaa aacttaccgt
     3421 ttttcccaat cggtagcagc ctacgaagaa tatctaaaaa ccataaatga aaccgacaag
     3481 gaattcttaa caattcaaaa agcaaaaaag aaagctgaac tggcagaaaa aatgattcaa
     3541 agagtggacg atattcaaat cattgatagt gtggtggtta ataaagataa ttttttggaa
     3601 ttttacaaat tcactaacga cttgggttcg ctatcggcaa cacctgtgaa aataggcaaa
     3661 aaaaaggatt ttaaaataac ttataccact caacgtcagg atagaaagtt gttttccgat
     3721 actgttcacg gtcaaatgga tatttttacc tcttacaagc ttttggatga atggtcaaaa
     3781 cccgaatcgg tttccgatat aatcaatacc aaagccaacg aaaattatcc ttttttactt
     3841 ttggacggtg ttaccatata ttttgcgtcg gacggagata attctattgg aggctacgat
     3901 ttgtttgttt cccgcttccg tccggaaacc aacacctatt ttcaacccga aaacattgga
     3961 atgccttata attcgattta caatgactat atgatggtta ttgacgacat tcatcaagtg
     4021 ggatggtttg ccaccgatcg ctttcagccg gaaggaaaag taattatttt ttcctttatc
     4081 cccaacacta aaaaaaccat tctccagtca gaggattcag aatatataag aaaagcagca
     4141 cagttacaac tattcaaaaa agccaaaaat ttcaattcgg atattattcc tcaaacatca
     4201 ttcgctgatg aagattcggt tcataaaatt ttctttgtag tgaacgattc tgtaatctac
     4261 acggatgaca atcagtttaa aaccgaagag acccgtaact tgtggaatga actggagaaa
     4321 ttaaattcgc aactaaatca aaaacgtatt caattggaac aactgagaat gcaatacagt
     4381 cagcaggaag aaactcaaaa tgaattggcg ccaaaaattc tgactttgga aaaggaaatt
     4441 tcggaaatgg aagatcagtt aactacaaaa acctttgaac tcagaaataa agaaaatata
     4501 aacttaatga agtagttgta tttcgaaatt tattttttat ttttaccaaa aattttaaga
     4561 caatggacat agcaatagct attattctga ttttggttgg tgtttttttc tttctggtag
     4621 aattttttct ggtgccggga atttcgtttg cgggaatagc cggttttttg tttgttggtg
     4681 ccggaattta ctattcctat tcccagttgg gaacaacagc agggaatgtt tctattgcag
     4741 gcagtgttgt tcttttggcc gttgcagttt ggattttttt gcgacgtaaa acattggaaa
     4801 gaatcagttt aaaggcaaaa atagattcca gtattggttc tgtcgaagaa ttgaacattc
     4861 atgcgggaga cacgggaatt gcgctctcaa gacttgctcc aatgggaaaa gtcaaagtaa
     4921 atggacaaac catagaagcc aaaagtgtag atgaatttat tgatgaagga accccaattt
     4981 tggtaaagga agttttcaaa accaatgtgt tggttgaacg tatttcggaa aattcaaata
     5041 atgaacatgc ttaaatttat aaattatgat gggaattgaa attgtattac tgattgttct
     5101 ggtcgttgtt ttattattgt ttttttacta tgtccctttt ttgttgtggc tctcggctaa
     5161 agtttccggt gtgagtattt ctctggtgca gcttttcctc atgcgcatca gaaaagttcc
     5221 gcctcactct attgttcaat gcttgataga agctcacaaa gccgatttgc acgaaataaa
     5281 gcgcgatgaa ctggaagctc actaccttgc cggcggacac atcgaaagag tggttcatgc
     5341 tttggtatca gcttctaaag ccaatattga cttaactttc aaaatggcaa ctgctatcga
     5401 tttggcagga agagacgttt ttcaggccgt tcaaatgtcg gtcaacccaa aagttatcga
     5461 tacaccgccg gtagctgcgg ttgctaaaga tggtattcag ctgattgcta aagcgcgcgt
     5521 tacggtgcgg gccaatatca aacagttggt tggtggagcc ggagaagaaa caatactggc
     5581 aagagtagga gagggaattg tttcctccat tggttcggcc gaatcgcata aggcagtttt
     5641 ggaaaatccg gattccattt ccaagttggt attgaaaaaa ggtttggatg ccggcacggc
     5701 atttgaaatt ttatccatag acattgccga tatcgacata ggaaaaaata ttggtgccca
     5761 gttgcaaatg gatcaggccg aagccgataa aaatattgca caggctaaag ctgaagaacg
     5821 tcgggctatg gctgttgccc ttgagcagga aatgcgggca aaatcacagg aaatgcgcgc
     5881 aaaagtaatt gaagctgaag ctgaagtacc taaagctatg gctgaagcat tgagaaacgg
     5941 gaatctgggt gtgatggact actacagatt gaaaaatatt gaagctgata ctgctatgcg
     6001 tgaaaatatc ggtaaaccct ccgataagac caaataaagt agcttctaaa tttaatggtt
     6061 actgaatctg aattgaacct tgtttgctga aaaattattt tgattggaaa caagtagatc
     6121 aaatctaaat tttaagaggg tgtcccaaaa ggacactttc ttttttaggc atataggtga
     6181 aaaagtagac tgaaatcttc ttgaaagttg aaaataagat atttatataa agaaattatt
     6241 cgatgtaatt gacttcgggt tgaagttgaa tatcaaattt gttttttact gaaagttgaa
     6301 tttcttcggc caattgttta atttcaatgc ccgaagctcc accacaatta acaattacca
     6361 aaggttgctt tgcataaaca gcgacttttc caacctgttt acctttccag ccgcattgtt
     6421 caataagcca tgcggcagga attttttctt ctgtttcaga tacataataa tgaggtattt
     6481 cgggatattc actttgcaaa cgctgaaaat gcactttgga aacgaccgga ttgacaaaaa
     6541 aactgccggc gttgcccatt gttctgaaat cgggtaattt ttggctcctt atttgtataa
     6601 tggtttccct aatggtttgc aggtttatgt ttcctctttt tctgacttct tcttccaaat
     6661 gctggtaatg aagcctgaaa ttaggtttct tcgacagacg gaagaccaca gaagtcaaaa
     6721 tgtatcgacc tttgtattga tttttaaaaa tactttgacg ataaccgtat tgacaatttt
     6781 ctttggaaaa ttccatcaat tgagaagttg cagtatcaat tgctttcact ttttcaatgg
     6841 cttcctgaac ttccataccg taagcgccaa tattctggac ggctgctgca cccacttctc
     6901 ccggaattga tgagagattt tcaatgccac tccagttatt ttttacacaa aattcgacaa
     6961 actgatccca cacaactccc gagcccactt caaaatagac aaattggtcg tcttccttta
     7021 tttttttcat ttgccgaatt ccggaatgta aaacgacacc cttaaaatcg ttcaaaaaca
     7081 gcaaattgct tccctgtcca atcacaaacc attttttttg ttttaatgtt ttcgacaaat
     7141 ctaaagcttc ttcaaccgtg taaaattcga taaattcttc ggcttttacg tcaatgccaa
     7201 aagtattgtg cgaaaggagg gaatagttgt atttaatatt cattcttgag aagttaagaa
     7261 agtcggtttt gtaggaaacg gattattttt tctctttcat cgggatgaaa ccaagtaatg
     7321 tctttgtcgt gattgaacca tgttaattgt cttttggcat aatgtcgggt gttttgttta
     7381 attttatcga cagcttgttt tagtgtaatt ttaccgtcca taaattcgaa aagttcctta
     7441 tatccaacag tgttcaaagg atttaagttc ttaaaagcgt aaaattgttt tgcttcctcc
     7501 aataagcctt cttccatcat ttgatctact cttgcattga tgcgttcata caattcatga
     7561 cgaggtctgt tcagccctat tttaacaata tcgaaagctc tttttttgtg aacgccggtt
     7621 cttaatgccg aaaaaggatt tccggtgatg gaacagattt ccagcgcatg caaaattctt
     7681 ttgtaattat ttgggtccac ttgttgataa tatacaggat ccaatttttt caattcttgt
     7741 cgaatgtatt caagtccctg attttcatat tgttttttcc agaagtttct tgtagtggta
     7801 tctactgtcg ggatgttgtc gagtcctttg catacagcat caatgtaaag cattgaacct
     7861 ccaaccatta gggcgatgtg attggtcgca aaatgttttt caagcaatgc gagagcttcc
     7921 tgttcaaatt gtccggcact gaaattttca aaaattgaat gagaaccgat aaaataatgt
     7981 tttactgtgg aaagttgttc ggatgtaggt gcagctgtac caattttcag ttctttgtat
     8041 atttgacgcg aatcggccga aatgatgggc gtttgtaaat tttgggctac ttgcaggctt
     8101 atttcggttt tacctacacc ggtagggcca agaattacta ttaaggttga ttgcattcgt
     8161 aaaaaataaa aaaacagaaa ataaacccgt tattttctgt cagtgaaaaa aatcagttga
     8221 aatcaaaagt tggtgtcatc attgaacaga ttgctgtcgt catcgaaatt gaaatcgcta
     8281 aatccttctt catccaactc atccagttca aaatcttcgt cgccataaaa gttttcttcc
     8341 acgttcagtt ttggagcctg attcacatca ttttcgctta aggtttgttc gggtggattg
     8401 ccgattgatt tgatgcaaac cggtttttca agtttttttc gtggaataat ttcacttagt
     8461 tctataaaaa atgctctatc agcaatcata tcaaacacat acatgagttt ttgtttctcg
     8521 tcggtaagca actcttccaa ttttacttta tccatcacca aattatcata ttctgaggaa
     8581 gaatccattt caataagggt aatttcctgc tctttttccc agtcgtcaga acagaggaaa
     8641 aaagaagtaa tctggtcttt tttgaagttt acggcatcca gaatggcatt atgtaagtca
     8701 aagaaagttg cttcagcatc tatatctatt actcttaaga aattgtccac ttcgtccgat
     8761 aatatagtaa atctaaaaac catagctttt gttgtttaag tttcgacggt aaaaatagta
     8821 atttttttgg agctaataag cattttctcc cgattttttc agaaaaaaag ccgtttaaac
     8881 aataattaaa cagcttttaa aagatattta ttgatcgaat agccataaat taatttcgaa
     8941 gtatttcctc aatagcctta acgttagatc tgaatttttt gtccgtatcc ataagatcac
     9001 taataatttt gcaggcgtgg agtacagttg cgtgatcgcg ctctccaatg gcttttccaa
     9061 tcgaagagag agaattttta gtgaactgtt tggacagata catggctatt tgacgcgcct
     9121 gaaccacttc tcgtttacga cttttggtaa aaatggcatc tacagggatg gagaaataat
     9181 cgcaaaccgt atcacgaatt ttttctaccg tatttatttt tttggggata tttacaatac
     9241 gactgacaac tttctcggca agttgcaggt cgatgggcat atctgtgagg gttgaatgtg
     9301 ccagcagtga aatcaaaacg ccttccaaat cacgaatatt atcggtaaca ttttgggcaa
     9361 taaaattcac tacatcttct ggtatatcca atccatcgca ataaattttg ttttttagta
     9421 tttctttccg aagttcgaag tcgggctttt cgatttcggc cgaaagtccc catttgaatc
     9481 gggtcagcaa acgttgttcc aatcctgcca tcagtaacgg agaacggtcg gaagtcaata
     9541 tcagttgttt gcccgattga tgcagatgat tgaaaatatg gaaaaaagtg ttttgggtgg
     9601 ccgtttttcc cgaaaattcc tgaatatcgt cgataatcaa aacttcgatg gtttggtaaa
     9661 aatttacaaa atcgttgacc gtattgtttc gtaccgcatc ggtgtactga atttggaaag
     9721 tattggccga aagataaagc actcttttat ctggatgcat ttgtttggct ttcaccccaa
     9781 tggcattggc taaatgcgtt tttcccacac ctgactctcc ataaacaaaa agaggattga
     9841 aagctgtttt tcccggttct gctccaatgg tgataccggc agtacgagcc agtttattac
     9901 tttttccttc tatcagagta ttgaaattat atttcatgtt caattgtgga tcgatattgg
     9961 gcaatgaaga tttgtcgtaa ggattttttg atccttgcgt ggcatttgag ttttttaatt
    10021 ttggctgttc agctgtactg gggatttgtg tgccttttcc attggtttga tcaatcaata
    10081 cacgatattc caattgagta ccggctccaa tttctctgta aatagttcta ctaagcaaat
    10141 cgatgtattt ttcctcgata tactcaacaa aaaattgact tggtacctgt acaacaaaac
    10201 gattgttttc atactgcaag gggataatag gttcaaacca agttttgtag gacgcttcgt
    10261 tgatattgtc cctgataact ttaaggcagc gttcccatag ttgttcgtgc atcttcattt
    10321 ttttaatcca ttcaaaaaag taaacaagtg agattttcag ccaaaaaaag attttcaaaa
    10381 ttttactttt gaaaattcta cttagacgta cggctgcatt tagttaggca aaaatctaaa
    10441 atttttttga caagaacaaa tctttttttc tacaaaaaaa atcagatttt tcatttggtt
    10501 aactgtcagt gctttgatga aacaaattca tattcagtta attgcataat aaatttttaa
    10561 aaattatcct gattctaaag gtgttaacaa aaagaaatca gaatttgaat tttttccgat
    10621 ttcttacatt gaaacctttt attaacaagt gtttcaatga tgttaaaaaa ataaattcaa
    10681 aattattttc aaatttttat ttagagaaat tctaaataaa ttctatttcg ttttgctccc
    10741 aaaaagtgag aagtgtacgt aacgtttcgg attatttttc aagtcgatca ataattgatt
    10801 ggcactggag gaagtatttg ccaaattcag ataaaattgc tggtcggtga gaagcatgcc
    10861 caaacttcct tctccgttat tgattttatt ggtaagcaaa ttcaaattgg agacagtttg
    10921 atcaacgttg ttcatagttg aagcaaaatt aatttctttc agattggaag ttacggattt
    10981 gaaatcggaa gaagctccat tcaaattact catgatagat ggaagctgat atttcatcag
    11041 atttttcatt tgtgccgaag tttctgccag gtcggcagtt gttttttcaa tggaagtgag
    11101 gctgttgttt aatttttcgc cttcggtaat agaacgaatg gaacgcaaaa gcgaatcggt
    11161 ttgtgtagaa atactttcaa tttttggtaa caaattggaa ctcaagtcgg ccatgagtcc
    11221 gggtttgatt tccgattgga gtgtatctcc gttgttgcaa aaattttgag aagaagcgta
    11281 gggcgcaaaa atcaactgaa tggcttttcc tcccatcaaa ccgttgtcga ataattgaat
    11341 cttggtgttt ttgggaagat tgattttttt ttcgatagta attttcacta aaaatggttt
    11401 ttcctttgaa aagtcgtatt gaatttcgtc aacctgtcca actttatatc cttttataaa
    11461 tacgggagaa gatgcaacca aaccatccaa attttcataa gtagtataat aagaacgggt
    11521 tgaggtaaaa atatcaattc ctttcagaaa gtttaaaccg aaatacaaca aaaaaatggc
    11581 tacaaaaacc atcaatccga tttttacttc tttattaaaa aaattttttt tcataacttg
    11641 aatattatca gaatttattt tttttcataa acaatttatt gtttttgcta tcaaaaagat
    11701 atttagagct tttcttagag tttttcagaa accacagttt tagaaatttt gttatttgcc
    11761 tgtcaagcgc aaagcctcct tgacatcaac ttttttaccg ttaacgaaag caacgataaa
    11821 agcttgggga aatttcgccg acaaattgtt ttttaattga acgattttat tgtaatcggt
    11881 ttcagctcca acggtgtatt tgtacagttc gttctcaatg taataatcaa cggattttag
    11941 gcctttaagt tcattcgctt ggggagataa tttctttttt gaagccagaa tttgtaattt
    12001 gaaaacaggt tgttgtgttg acggtaaaat attttctgtt tgtttttctt gattcgtaat
    12061 gttggcagta ttgatgtttt caatttctgc ttgctgtata ggagttgaat aattgttttg
    12121 actttctgaa actatgtttt ccgatttttt ggaaggagct tgattggaaa cagacgtaga
    12181 atttggcttg ccgtttcgct tgtcgtgttc ttttttgaac tctaaaaatg cggtgtagat
    12241 agcttgagcc atcttttttt gtccatattc ggaacttaaa aaaagttctt cttccagatt
    12301 ggaaataaat ccgagttcta tcagtgtgct gggacaggca gagcgatgaa gtacccaaaa
    12361 tccggcctga cgaacgcctc tgtccaaacg tccgtttcta tcgaggtgtt gttgcaccaa
    12421 cgaagcaaat tcgatacttt tatccagata tttgtcctgc ataaattcaa acattatata
    12481 ggaatcaacc gaagtcgggt cgaaaccctg gtatttggtt tgatagtttt cttccagcaa
    12541 aatcactgag ttttcacgca tggcaacttc aagatttgca tttgttttgg ccaaacccag
    12601 tgtataagtt tctgccccgt aagccgaaga acttttcgaa gaattggtat gaatgcatat
    12661 aaaaagatca gcatgatttt cgttggctat gtcggcgcgt tcctgcaggg taagatattt
    12721 atcggttttt cgtgtgagta caactttcaa ttccttattg ttttccatga gtttagccaa
    12781 ttctttggca acagcgagat tgatatcttt ttctcttgaa attttaccca ttgctcccgg
    12841 atcatgtcct ccatgtccgg catcgattac cactacaaat tttttttcct gcgcagcaac
    12901 actggaagcg aaaatggata aaaacaatat caggaaaccg taaggcaaca ataagtactt
    12961 ttgaaatgga attttcatgc caaaaaattt ttttgttttt tttgcatcaa attatttttt
    13021 aactgctaat gaactttttt atgggttcat ctttcactat gtttttacaa tccttttatt
    13081 ttctgcaaaa ttaatctttt ttatttcaaa ttccattgga taaatttgaa attaatcata
    13141 aaaaagcggt tggatactgt agatagctat ttttttcctt aaatttgcag cttaattgaa
    13201 aatgtttaat cccaaggatg cactataatt ttaaatttca caacagcctg aaatgtaaat
    13261 tattcctctt aattacaggg ttgaatattt cattttgt
//
LOCUS       DAOSCH010000056        13129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3203_length_13129_cov_6.456937, whole genome shotgun sequence.
ACCESSION   DAOSCH010000056 DAOSCH010000000
VERSION     DAOSCH010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13129
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3203_length_13129_cov_6.456937"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            296..1576
                     /gene="metK"
                     /locus_tag="PLZ54_05580"
     CDS             296..1576
                     /gene="metK"
                     /locus_tag="PLZ54_05580"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008769127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOJ66432.1"
                     /translation="MAYLFSSESVSEGHPDKIADQISDALLDNFLALDRDSKVACETL
                     VTTGQVIIAGEVNTTAYVDVQSVARRVINRIGYTKSDYMFDGNACGILSALHDQSPDI
                     QRGVEREDPIEQGAGDQGMMFGYACNETSNYMPVALDLAHALVGELAKIRKEGKQMTY
                     LRPDAKSQVTIEYDDSNRPLRVHTIVISTQHDEFIQPSLTKSQDEADREMLLIIRQDV
                     QNILIPRVKLLDKQFAELFKDNFTLLVNPTGKFVIGGPHGDTGLTGRKIIVDTYGGKG
                     AHGGGAFSGKDPSKVDRSAAYAARHIAKNMVAAGVADEVLVQVAYAIGVADPINLYID
                     TFGTSKVKFSDSEIAEKINNIFDMRPKAIEERLKLRYPIYEETASYGHMGRTPMEVRK
                     VFKDGNGNQITKTVELFTWEKLDKVKEIKSIFQL"
     gene            1969..3204
                     /gene="trpB"
                     /locus_tag="PLZ54_05585"
     CDS             1969..3204
                     /gene="trpB"
                     /locus_tag="PLZ54_05585"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOJ66433.1"
                     /translation="MPENSQKSRNGADRKLYQPDEKGFYGEFGGAYIPEILYASVEQL
                     KKAFLSIKGDENFKNEYYDLLKNYVGRPTPLYFARRLSELYGVNIYLKREDLNHTGAH
                     KINNALGQVLLARKMGKTHMLAETGAGQHGVAAATACALMGMDCTVFMGKTDIERQYL
                     NVQKMRMLGARVEPVTSGNMTLKDATNEAIRYWCSHPDSYYIIGSTVGPDPYPEMVGY
                     FQSVISEEIKWQLQEKIGRNYPDYLMACIGGGSNAMGTYFHFLDDERVNIISAEAGGM
                     GIETGKTAATIHLGKTGIIHGAKTLILQNEDGQIDEPYSVSAGLDYPGVGPAHAFFTK
                     IGRTKVYAINDDEAMEAAFELTRLEGIIPAIESAHALVILKKEKANFRSDEVVVLTVS
                     GRGDKDINTYVKYFESTTK"
     gene            3270..4697
                     /locus_tag="PLZ54_05590"
     CDS             3270..4697
                     /locus_tag="PLZ54_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HOJ66434.1"
                     /translation="MKLYVKTKKMLADLETPVGIYLKIRDLYTHSVLLESSDYHAAEN
                     SYSFIAFSPIGGISVNHFLVHEYYPDGTKIQTPVTDKMTVADRFDIFLKSFERIEQDN
                     EVPAVVNGLFGYTSYEAVQYFEDIRLSARETVPGSMPGLHYVLFKYIIAVNHFKNELT
                     IIENLLENEKSAMNEIEEILLNNNIAAYEFSGIGSEKCDINDEDYRRMVKRGKEECFK
                     GNVFQIVLSRRFYQQYQGDDFMLYRTLRSINPSPYLFYFDFADFRIFGSSPEAHLVVD
                     AKAQKAYIKPIAGTFRRTGDDIKDLELAEKLKNDEKENAEHVMLVDLARNDLSRNADE
                     VKVETFREVQYYSHVIHLVSCVSGKLRPGTKIMKFFADTFPAGTLSGAPKIKAMQLID
                     QIEPHDRGPYGGCIGYIGFDGGFNQAITIRSFISKNNTLFYQAGAGIVAKSDEENELQ
                     EVNNKLAALKQAIILAGKRNNYQTE"
     gene            4783..5364
                     /locus_tag="PLZ54_05595"
     CDS             4783..5364
                     /locus_tag="PLZ54_05595"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HOJ66435.1"
                     /translation="MKLLVFDNYDSFTYNLVHIVKKLGFTDVEVHRNDRISLEEINRF
                     DKIILSPGPGVPSESGILLELIKTYASSKSIFGVCLGVQAIAEAFGGSLLNLPQVYHG
                     IASEVSIFDDEVLFRNLPEKIIAGRYHSWVVNDSTLPPCLQVTAKDEQGTIMALKHRT
                     LDVRGVQFHPESVLTPSGEQILKNWLEFPLSGK"
     gene            5443..6441
                     /gene="trpD"
                     /locus_tag="PLZ54_05600"
     CDS             5443..6441
                     /gene="trpD"
                     /locus_tag="PLZ54_05600"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOJ66436.1"
                     /translation="MKQILNRLFNHQSLTREESAEVMRNIGAGMYNESQIAAFISVYL
                     MRSIELDELIGFRDALMSMAIPMDLSDFDPIDIVGTGGDEKNTFNISTCSCFVLAGAG
                     YKVAKHGNYGATSISGSSNVLEYYGAKFTTDTDVVRRSLEESGFAYLHAPFCNPAMKN
                     VAPVRKNIGVRTFFNVLGPLISPIRPRYQCLGVYNLKMMRLYNYIYQRLGTNYTIVYT
                     LDGYDEVSLTDDCKIINNFGEYVYSPQQLGFQRVKQEELWGGTTVEHAAEIFMNVLDN
                     KASDAQRNVVVINSAFAIQTRCPEKSLEECKAEAIASLACGKAKKAFVKFEEIYHL"
     gene            6451..7278
                     /gene="trpC"
                     /locus_tag="PLZ54_05605"
     CDS             6451..7278
                     /gene="trpC"
                     /locus_tag="PLZ54_05605"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOJ66437.1"
                     /translation="MTDSTLKNNHLPNPGENFLNNILLNKIKEVNKRKLNVSITELEN
                     SNFFDRSTISLKQKLQNSSLGIIAEFKRKSPSKGWIHADANVQEVTIGYNVEGASGIS
                     ILTDTEFFGGNIEDVTTVRNKISCPILRKDFVVDEFQIYEAKAIGADVILLIASALTI
                     YEVKNFSRLAHQLGLEVLLEVHNKEELSYLNENVDMVGVNNRNLKTFQVDTGISANLA
                     AFIPPEFVKISESGISNVETVKELNRLGYSGFLMGENFMKEVHPALALKEFLRQLNE"
     gene            7430..8113
                     /locus_tag="PLZ54_05610"
     CDS             7430..8113
                     /locus_tag="PLZ54_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOJ66438.1"
                     /translation="MRSVEKNNIVPSRFADTTKKKLIKVCGMKFPENIRAVARLRPDF
                     MGFIFYPKSPRYAEPLNVSVLNELPPEIGKIGVFVNEKLDQILATVYKYHLDGVQLHG
                     SEMVKMCNQLHDTGLLVIKAFPVAEAYNFKVMKRYEKSCDYFLFDAQTPIYGGAGQKF
                     DWQLLENYKEKTPFLLSGGISIEDLEDINKIQHPAFAGVDLNSRFEISPGLKNVELLS
                     NFIDRYRSL"
     gene            8140..8910
                     /gene="trpA"
                     /locus_tag="PLZ54_05615"
     CDS             8140..8910
                     /gene="trpA"
                     /locus_tag="PLZ54_05615"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOJ66439.1"
                     /translation="MNRIQQLFTQKKENILSVYFTAGFPKPNDTVPILESLQKYSVDM
                     AEIGIPFSDPLADGPVIQNSSQIALKNGMNLKKLFSQLANIRASVHIPLVMMGYLNPI
                     MQFGFENFCSECKKVGVDGMIIPDLPMDVYLTEYREIVEKNGLDFIFLITPETSEERI
                     RQIDEHTHGFIYMVSSAGVTGTQNSFDHHIDYFNRINSMHLKNPRLIGFGISNKLTRE
                     TASRYSSGVIIGSAFIKALQESSDVESAVKLLIKKLED"
     regulatory      9339..9434
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9486..11912
                     /locus_tag="PLZ54_05620"
     CDS             9486..11912
                     /locus_tag="PLZ54_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ66440.1"
                     /translation="MRQFVFTAILLFGTLMSFGQHWIKGKVLDENGHPLVGADIYLQK
                     EGIGKTTDKNGTFLFENLKNKNYFLQVSYLGYETQWVETTPDKEITIQMNRISIEIDE
                     ITVTSLRANERSPIAYSEIKKEEIESRNLGQDIPYLLALTPSFIVTSDAGNGVGYTGF
                     RIRGTDANRTNVTINGIPLNDAESHGTFFVDFPDLAASLSSVQVQRGVGTSTNGAAAF
                     GASINMQTESVSLEPYADLSTSLGSFATNKNSIKVGSGMLDNNFAFDARLSNVTSDGY
                     IDRASVDMQSYYFSGGYFSDKTVLRLLTFGGKEKTYQAWNGVDAEIMKTNRRYNDLGA
                     YTDNNGELKFYDNQTDNYLQTHYHLYWLQKWNSHLHSNVATHLTRGIGYYEEYKIDKK
                     YAEYGLTPAIIGFDTLKTTDLVRQKWLDNYFYGITGALNYEKKNTNFSLGGAVNRYDG
                     EHYGKIIWVRNAANLDIGKDWYRSSSIKDDANIYAKLNTETFKNLFASIDLQYRYINY
                     RMDGTDDKYDKINQSMRDITQQHIYHFFNPKFGFTWQPSTRNEFYASYSVSHREPNRN
                     NFTEAGANERPTNETLYDTEAGYRFRSSRFSAGTNFYYMKYKNQLILTGKVSEIGEFL
                     TTNIPDSYRAGIELTAGWKITNFLNWDGNCTFSHNKIKNFTEQDIDVYDENWNYIGTR
                     NNELGTTDIAYSPNVIANSIFSFHLKPFEAAFHSNYVSRQYIDNTSDIQRSIDPYFVQ
                     NLNLKYSLAISPIKTLEFLVQINNLFNEKYETNGYTWYSCYLGNERHNELRYFPQAGT
                     NFLASARLKF"
     gene            complement(12170..>13129)
                     /locus_tag="PLZ54_05625"
     CDS             complement(12170..>13129)
                     /locus_tag="PLZ54_05625"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HOJ66441.1"
                     /translation="SLWVPDKKYDFKDVITGYSSLKGFQQSTEVYFGALIGRYGNRIA
                     KGRFELNGKTYQLPINDGENHLHGGPKGFHTVIWDARPFKNANNEDALELKYFSRDGE
                     EGYPGNLTVKVVYTLTNDNELKIEYSATTDKPTIINLTNHMFFNLHGFSDGIAKSINS
                     HVLEINASNYTPTDKGLIPTGIIAPVKGTPMDFTVPTPIGERVNENFIDLINGKGYDH
                     NWVLNKKGDKLTEAAVVHEPSNGIQMRVITDQPGVQFYGGNFLNGTERGKYGEVYTYR
                     TSFAMETQHFPDSPNHPNFPSTVLNPGQQYQHVCIYKFEVGGN"
BASE COUNT         4127 a         2498 c         2621 g         3883 t
ORIGIN      
        1 aaaaaagtaa gtggggtttg gggcaaagcc ccgttattaa atatctgaaa ataaaaaaca
       61 taaatgttta tacaattttt ttgtcatata cataagtaca aatttagtaa aaacaaagaa
      121 ttttgattaa ttttgcacag aatttattta taagtggaaa gatttggctg cttgcaacca
      181 cgtaaaaaaa tgctaaaatg attcaatttt ctttctcctt ttttagctgc cttctcttca
      241 acttacaaag taaattcaga catttgattt ttaagtaatt taataattta atgatatggc
      301 ttatttattt tcgtctgaat cggtgtcaga aggtcatccc gataaaatag ccgatcagat
      361 ttcagatgct ttgttggata attttttggc tttagatcgt gattctaaag tggcttgtga
      421 aacattggtt actaccggtc aggtaattat agctggagaa gtaaatacca cagcttatgt
      481 tgatgtacaa tcggttgcgc gtcgtgtaat taatcggatt ggttatacaa agagcgatta
      541 tatgtttgat ggaaatgctt gtggcatttt atctgcacta cacgatcaat cgcctgatat
      601 tcagagagga gtagaacgag aagaccccat agaacaagga gctggtgatc agggtatgat
      661 gtttggatat gcatgcaatg aaacttccaa ttacatgcct gttgcacttg acttagcaca
      721 tgctttggtg ggagagctgg ctaaaatacg caaggaaggt aagcaaatga cttatcttcg
      781 tcccgatgcc aagtcacaag taactattga gtacgacgac agcaaccgcc ctttgagggt
      841 tcatacaatt gttatttcta ctcaacacga tgagtttata caaccttcgc tgaccaaaag
      901 tcaagacgaa gccgacaggg aaatgttgtt gattatccgt caggatgtgc aaaacattct
      961 tattcctcgc gttaaactgc ttgataaaca gttcgccgaa ttatttaagg ataattttac
     1021 attactggtc aatccgacgg gaaaatttgt tatcggaggt cctcatggcg acactggatt
     1081 gacaggaaga aaaataattg tagataccta tggaggtaag ggagcacatg gtggtggcgc
     1141 tttttcgggt aaagatccat ctaaagtgga tcgctcagca gcttatgccg ctcgacacat
     1201 agctaaaaac atggtagctg ccggcgtcgc cgacgaagtt ctggtacagg tagcttatgc
     1261 cattggtgtt gctgatccga taaatcttta cattgatact tttggcactt caaaagtaaa
     1321 attttctgat agtgaaattg ccgaaaaaat aaataatatt tttgatatgc gacctaaagc
     1381 catcgaggaa cgattaaaat tgagatatcc aatttatgaa gaaacagctt cgtatggaca
     1441 catgggcaga acaccaatgg aagtgagaaa ggtttttaaa gatggaaatg gaaaccaaat
     1501 tacaaaaaca gttgaattat ttacatggga aaaacttgac aaggtgaaag aaataaagtc
     1561 gatttttcag ctttaaaaag tataatggag aatatttcat aaactgtgta aatttttaag
     1621 attcaaaata ttaaattaca ctttttgcaa gtaagatgag gatgatttat tgagcaaaat
     1681 acttgtaagt tggatattaa taattttctg aattaaaaat tgattttttt gttgcacaat
     1741 taaaaataat ttgtcaactt tgcaatcgaa aacaaaacag actgtaaaaa atgaaaacaa
     1801 attcgacaat caacaaattt tggtggtggc gcttgcgaat attcagatcg tgagaaggga
     1861 cacctatgtt gatagttgaa ataaagaaat atttatgaaa aggcttgtcg ggaactcacg
     1921 acaagccttt tttcattgat aaaaatacaa tcaaaaaaca attaaactat gcccgaaaat
     1981 agtcaaaaat cgagaaacgg agctgataga aaactttacc agccagatga aaaaggtttt
     2041 tatggtgaat ttggcggagc gtatattcct gaaattctat atgcttccgt cgagcagttg
     2101 aaaaaagcct ttttatccat aaaaggggat gaaaacttta aaaatgagta ttacgattta
     2161 ctcaaaaact atgttggcag accaacacct ctgtattttg cacggcgact ttccgaatta
     2221 tatggtgtca atatttacct gaaacgtgaa gacttgaacc ataccggtgc acataaaatc
     2281 aacaatgcac tgggacaagt actattggcc cgaaaaatgg gtaaaacgca catgctggcc
     2341 gaaaccggag ccggacaaca cggtgtggct gctgcaactg cttgcgcttt gatgggaatg
     2401 gattgtaccg tttttatggg aaaaaccgat atcgaacgtc aatatctgaa cgtgcagaaa
     2461 atgcgaatgc tgggtgcaag agtagaacct gttaccagcg gaaacatgac cttaaaagat
     2521 gcaaccaacg aagccattcg ttactggtgt tcgcatcccg attcttatta cattatcgga
     2581 tcaacagtcg gtccggatcc gtatcctgaa atggttggct attttcaatc ggttatcagc
     2641 gaagaaatta aatggcaact gcaggaaaaa attggacgaa attatcccga ttatttgatg
     2701 gcctgcatag gcggaggaag caacgcaatg ggaacttatt ttcattttct ggatgatgaa
     2761 agggtcaata tcatttcggc cgaagccggc ggaatgggaa ttgagaccgg aaaaacggct
     2821 gctaccattc acttgggaaa aaccggcatt attcacggag caaaaacctt gattttgcag
     2881 aatgaagacg gacagattga cgaaccatat tccgtttctg ccggtttgga ttatcccggc
     2941 gttggacctg cacatgcttt ttttacaaaa attggtcgta ccaaagttta tgccatcaac
     3001 gatgatgaag ctatggaagc agcctttgaa ctgactcgtc tggaaggaat tattccggct
     3061 atcgaatcgg ctcacgcact ggttattttg aagaaagaaa aagccaattt ccgttctgac
     3121 gaagtagtag tgcttaccgt ttcgggaaga ggagataaag atataaatac atacgtaaag
     3181 tatttcgaat caacaactaa ataatttttc atttttaaat tttatctgcg ctgttttatc
     3241 ctgatattca gctaaaaaat ttcaataaca tgaaactata tgtaaaaaca aaaaaaatgt
     3301 tggccgattt agaaacgccg gttggcattt atctaaaaat cagagattta tacactcatt
     3361 cggttttgtt ggaaagttcc gattatcatg cggccgaaaa tagttattct ttcattgcgt
     3421 tttctcccat tggaggaatt tccgtcaatc atttcctggt acatgaatat taccccgatg
     3481 gtacaaaaat tcaaactccc gtaacggaca agatgacagt agccgaccgt tttgatattt
     3541 ttctgaaatc attcgaacgt atcgaacagg acaacgaagt tcccgctgta gtaaacgggc
     3601 tttttggcta tacttcttat gaagcggtgc agtattttga agacatccgg ctttcggcac
     3661 gcgaaactgt tcccggaagt atgccgggtt tgcattatgt gctgttcaaa tatatcattg
     3721 ccgtcaacca ctttaaaaat gagttgacaa tcattgaaaa tttgctcgag aacgaaaagt
     3781 cggccatgaa cgaaatagaa gaaatactcc tcaacaacaa tattgccgcc tacgaatttt
     3841 cgggaattgg cagcgaaaaa tgcgatatta acgacgagga ctaccgtcgg atggtaaaac
     3901 gcggaaagga agagtgtttc aagggaaatg tttttcaaat agtcctttca agacgtttct
     3961 accagcaata tcagggagac gatttcatgc tttatcgtac cttgcgttct atcaatcctt
     4021 ctccctatct tttttatttc gattttgccg atttccgcat ttttggttct tcgcccgaag
     4081 cccatttggt agtcgatgcc aaagctcaaa aagcctacat aaagcccatt gccggaactt
     4141 ttcgccgcac cggagacgac attaaagacc ttgagttggc cgaaaaactg aaaaacgacg
     4201 aaaaggaaaa tgccgaacat gtaatgctgg tggacttggc acgcaacgat ctgagccgaa
     4261 atgccgacga agtgaaggtt gaaacttttc gggaagtgca atattattca catgttatcc
     4321 atctggtttc gtgcgttagc gggaaactgc gtcccggaac aaaaatcatg aaattttttg
     4381 ccgatacttt tcctgccgga acactttccg gcgctccaaa aattaaagcc atgcaactta
     4441 tcgatcaaat tgaaccacac gaccgcggcc cttatggagg ttgcattggc tacattgggt
     4501 tcgatggcgg attcaaccaa gccattacca ttcgctcttt catcagcaaa aacaacacgc
     4561 ttttttatca ggcaggtgcc ggcattgtgg ccaaatcgga tgaagaaaac gaattgcagg
     4621 aggtaaacaa taagttggct gcactgaaac aagccattat attggccgga aaaagaaata
     4681 actatcaaac agaatagatt ttaataaacc gatggcctat cgaacaagtt tcagaatttt
     4741 ctccgaaaac tttaaataga ccgaaaaaat aatgcaacct gtatgaaatt gctcgttttt
     4801 gacaattacg attcctttac ctataatttg gtacacatag ttaaaaagtt ggggttcact
     4861 gatgtggaag ttcatcgcaa tgatcggatt tcgttagaag aaataaaccg tttcgacaaa
     4921 attattcttt ctcccggtcc gggcgttcct tccgaatcag gcattttact cgaattgata
     4981 aaaacctatg cttcatccaa aagcattttt ggcgtgtgtc tgggtgttca ggcaattgcc
     5041 gaagctttcg gtggaagctt gctcaatctt cctcaggttt atcacggcat tgcttccgaa
     5101 gtcagcattt ttgacgatga agttttgttc agaaatttgc cggaaaaaat tattgccggc
     5161 agataccatt cctgggttgt aaacgattct acactgccac catgtctgca ggttacggca
     5221 aaggacgagc agggtaccat catggcgctg aaacatcgca cccttgatgt tcgcggcgtt
     5281 cagtttcatc ccgaatcggt gctgacacct tccggtgaac aaatactgaa aaattggttg
     5341 gaattccctt tatcggggaa ataatagatt taaaaaatta tagagaaaca aatcagagaa
     5401 aacaagtttt ttcctcattt tcagcaaaaa aacagttaac ttatgaaaca gatattaaac
     5461 agattattta atcatcagtc gttgaccaga gaagaatcgg ccgaagtaat gagaaatatt
     5521 ggagccggca tgtacaacga atcgcaaatt gccgctttta tttcggtata tctaatgcgc
     5581 agcatcgaac tggacgaact gatcgggttc cgtgatgcct tgatgtcaat ggctattcca
     5641 atggacttat cggatttcga tcccattgat attgtgggaa cgggcggtga cgaaaaaaac
     5701 accttcaaca tttcaacctg cagttgtttt gttttggccg gtgccggtta caaagttgcc
     5761 aaacatggaa attatggagc aacatctatc agtggctctt caaacgtgtt ggagtattac
     5821 ggcgctaaat ttaccaccga tactgatgtt gtacggcgct cactggaaga atcgggtttt
     5881 gcctatttgc atgctccatt ctgcaatccg gcaatgaaaa atgttgctcc cgtccgtaaa
     5941 aatatcggcg ttcgcacctt cttcaatgtg cttggcccgt tgatcagtcc cattcgaccg
     6001 cgttatcagt gcttgggagt ttacaacctg aaaatgatgc gcttgtacaa ctatatttat
     6061 cagcggttgg gaacaaatta taccattgtt tacactctcg acggatatga tgaagtttca
     6121 cttaccgacg actgcaaaat aatcaacaac tttggcgaat atgtttacag tccccaacaa
     6181 ctcggctttc agcgagtaaa gcaggaagaa ttgtggggtg gcacaacagt ggaacatgca
     6241 gccgaaatat ttatgaatgt attggataac aaagcttccg atgcccagcg aaatgtagtg
     6301 gtcattaatt ctgcatttgc catacaaacg cgctgccccg aaaaatcgct cgaagaatgc
     6361 aaggcagaag ccatcgcatc gctcgcctgc ggaaaagcaa aaaaagcatt tgtgaaattt
     6421 gaagaaatat atcatttatg atttctacca atgactgatt caacattaaa aaacaatcac
     6481 ttaccgaacc caggagaaaa tttcctgaat aatattcttc tgaataaaat aaaggaagtt
     6541 aacaaacgaa agctaaatgt gtcaattacc gagttggaaa attctaattt ttttgatcga
     6601 agcactattt cgctgaaaca gaaattacaa aattcttctt tgggaattat tgccgaattc
     6661 aaacgtaaat ctccttccaa aggttggatt catgccgatg ccaatgtgca agaagttact
     6721 atcggctaca atgtggaagg agcttcagga atttctatcc ttaccgatac cgaatttttt
     6781 ggtggaaata ttgaagatgt aacgaccgtt cgcaataaaa tttcctgtcc cattttacgg
     6841 aaagactttg tggtggatga atttcaaatc tatgaagcca aagccattgg cgccgatgtg
     6901 attcttctga ttgcatcagc cttaaccatt tatgaagtta aaaatttttc acggttggct
     6961 caccaattgg gacttgaagt tctgctggaa gtgcacaata aggaagaatt gagctatttg
     7021 aacgaaaatg tagatatggt tggcgtaaac aatcgaaatc ttaaaacttt tcaggtggac
     7081 acgggcattt cagcaaattt ggcagcgttt attcctcctg aatttgtaaa aatcagtgaa
     7141 agtggcattt ccaatgtcga aactgtgaaa gaattgaatc gattgggata cagcggcttt
     7201 ctgatgggtg aaaattttat gaaggaagtc catccggctc ttgctctgaa agagtttttg
     7261 agacagttaa atgaataata ttacaattga atgaagataa ttagcagctt tagattattg
     7321 actcgattag tcgatatatt tgtaaatttg tttcataaat attttcctga caaataattt
     7381 tgttaaacct cttaatcact gcaaaagtta cattacttga attttcttta tgagatcagt
     7441 agaaaaaaac aacattgttc cgtctcggtt tgcagatacg acaaagaaaa aattgataaa
     7501 agtctgcggg atgaaattcc cggagaatat tcgtgccgta gcccgtctcc gtcccgattt
     7561 catgggtttt atcttttatc ccaaatcgcc gcgttatgcc gaacctttaa atgtttccgt
     7621 tttgaatgaa ttgcctcccg aaatcggtaa aattggtgtt tttgtcaacg aaaaactcga
     7681 tcaaatcctg gctacggttt acaaatacca tttagacggc gtacaactgc atggaagtga
     7741 aatggtgaaa atgtgcaacc aattgcacga tacgggattg ctggtcatca aggcttttcc
     7801 tgttgccgaa gcttataatt tcaaggtaat gaaacgctac gaaaaaagtt gcgactattt
     7861 ccttttcgat gcacaaactc ccatttatgg cggggccggg cagaaattcg actggcagtt
     7921 gctggaaaat tacaaagaaa aaacaccttt tcttctcagt ggcggaattt cgattgaaga
     7981 tttagaagat attaataaaa ttcagcatcc ggcttttgca ggcgtcgatc tgaacagccg
     8041 tttcgaaata tcgcccggct taaaaaatgt cgaactttta agcaatttta ttgatcggta
     8101 tcgttcactt taacaagaaa aatacataaa taattcatta tgaatagaat tcaacagtta
     8161 ttcacgcaaa aaaaagaaaa cattctctcg gtttatttta cggccggctt cccaaaaccg
     8221 aatgacacag ttccaattct ggagagttta caaaaataca gtgtggatat ggccgaaatc
     8281 ggtattcctt tttccgatcc gcttgccgat ggacctgtta ttcaaaacag cagccagata
     8341 gctttgaaaa acggcatgaa tctaaagaaa cttttcagtc agcttgccaa cattcgtgct
     8401 tcggtgcaca ttccactggt aatgatgggt tatctgaatc cgattatgca gtttggtttt
     8461 gagaatttct gcagcgaatg taaaaaagtc ggagtggatg gcatgattat tcccgatttg
     8521 cctatggacg tttatctgac ggaatacaga gaaatagtcg aaaaaaacgg cttggatttt
     8581 atttttctga ttacccccga aacctcagaa gaacggattc gtcagattga tgaacacacg
     8641 catggtttta tttacatggt ttcttctgcc ggcgttaccg gcacccaaaa ttcatttgac
     8701 catcatattg attatttcaa tcgcattaat tcgatgcatt tgaaaaatcc ccgactcatt
     8761 ggctttggca tttccaacaa attgacccgc gaaaccgctt cgcgttactc ttcaggtgta
     8821 attatcggca gtgctttcat caaagctttg caggaaagtt cggatgtgga gtcagccgta
     8881 aaattgctga taaaaaaatt ggaggattaa tttcgacttt cgagtcaaat tgcgtgaaga
     8941 ataaattctt tgcatcaaaa aaaattctgt ccggcaaaat gctggaaatt caccggacag
     9001 aatttttatt tttcctgtca tttgcacttt tatcgaattt cgaatataaa aagttgtttt
     9061 ctaattagtt acttattttt tggttccctt tgtagttttt ctatcgtttt ttttactgta
     9121 tttcatgcat gttgatttca gcaaatcaat acaacttttt caattatatt attgatattc
     9181 aattgaatag aaaaaattaa aaacgacttt ttcccttcgt ataatttttt gataaaaaaa
     9241 tatcaatttc ttgaatttaa acttttattg ggaaagattt tgtaactttg caacttgaaa
     9301 aagagaaatc cgcggttttt cttcatcaac ttaatttttt tattaaaatg gggtgcctta
     9361 ataatttcgg gctgagatca taccctttga acctgcacgg gtaattccga gtagggaata
     9421 agttttcaat caaccgtttt ttaggctctc ataacaaagt attaaccatt aattaatttt
     9481 ttgtgatgag acaatttgtt tttacggcaa ttttgctgtt tgggacgtta atgtcctttg
     9541 gtcaacactg gataaaagga aaagtgttgg atgaaaatgg tcatcccctc gttggggcag
     9601 atatttacct gcaaaaggaa ggaatcggta aaacaaccga taaaaatggt acttttcttt
     9661 ttgaaaattt aaaaaataaa aattattttc tgcaagtttc ctatttaggt tacgaaaccc
     9721 aatgggttga aacgacaccg gataaagaaa tcacgattca aatgaatcga atttccattg
     9781 aaatagatga aattacggta acttcgttgc gtgccaatga gcgttcgccc attgcttaca
     9841 gtgaaataaa aaaagaggaa atagaaagcc gaaatctcgg tcaggatatt ccttatcttt
     9901 tggcactgac accttcattt atagtaactt ccgatgccgg aaacggtgtt ggttacacag
     9961 gatttagaat cagaggaaca gatgcaaacc gaacaaatgt tactattaat ggtattccgt
    10021 tgaatgatgc cgaatcgcac ggaacatttt tcgtcgattt tcccgatctg gccgcatcac
    10081 tatcatcggt acaggttcaa cggggcgtcg gcacttcaac caatggcgca gctgcttttg
    10141 gcgccagcat caatatgcag accgaaagtg tttcattgga accgtatgcc gacctcagca
    10201 cctctttggg ctcttttgca acaaacaaga actccattaa agttggatcg ggcatgctgg
    10261 acaacaattt tgcatttgat gctcgcctct cgaacgtaac ttccgatggt tatatcgacc
    10321 gcgcttcggt agatatgcaa tcctattact tttccggcgg ctatttttcg gataaaacag
    10381 ttttgcgact gctcaccttc ggaggaaaag aaaaaactta tcaggcatgg aacggtgtag
    10441 atgccgaaat aatgaaaaca aacaggagat acaacgattt gggcgcctat accgacaaca
    10501 atggagaatt aaaattctac gacaaccaaa ccgacaatta tttgcaaact cattatcatc
    10561 tctactggtt gcaaaaatgg aacagccatc tccattcaaa tgtagctacc catctgactc
    10621 gcggaatcgg ttattatgaa gagtataaaa tcgacaaaaa atatgcagaa tatgggctca
    10681 cacctgccat tataggtttt gatacgctga aaacgaccga tttggttcgg caaaaatggc
    10741 tcgacaacta tttctatggc attaccggag ctttgaatta cgagaaaaaa aacacgaact
    10801 tttcgcttgg cggagctgtc aaccggtatg atggagagca ttacggaaaa ataatttggg
    10861 ttcgcaatgc agctaatttg gacattggga aagactggta cagaagtagt tccataaaag
    10921 acgatgccaa tatttatgcc aagttgaaca ccgaaacttt taaaaacttg tttgcttcaa
    10981 ttgatttgca ataccgatat attaactatc ggatggacgg aaccgatgac aaatatgata
    11041 aaataaatca atccatgcgg gacattaccc aacagcacat ttaccatttt ttcaatccga
    11101 aattcggatt cacatggcaa ccaagtaccc gaaatgaatt ttacgcttcc tattccgtat
    11161 ctcacagaga accaaacagg aacaatttta ctgaagccgg agcaaacgaa cgtccaacca
    11221 acgaaacatt atacgatacc gaagccggct atcgtttccg atcgtcccga ttttcggccg
    11281 gcaccaattt ttactatatg aaatataaaa atcagttgat tctcacggga aaagtaagtg
    11341 aaatcggaga atttctgact actaacattc ccgacagcta tcgggcagga attgaactga
    11401 cagcaggttg gaaaattact aactttttaa actgggacgg aaattgcact tttagtcaca
    11461 ataaaatcaa aaattttact gaacaggata tcgacgtgta tgatgaaaac tggaactata
    11521 tcggcacacg caacaacgaa cttgggacaa ccgatatagc ctattctcca aacgtgattg
    11581 ctaacagtat tttttctttt catcttaagc ctttcgaagc ggcttttcat tcaaattacg
    11641 ttagtcgaca gtatatagac aacacttcgg atattcaacg ttctattgac ccatattttg
    11701 ttcaaaattt gaatttgaaa tactcgcttg ctatcagtcc tatcaaaacg ctcgaatttc
    11761 tcgttcaaat caataatctc ttcaacgaaa aatacgaaac gaacggttac acatggtatt
    11821 cctgctatct gggcaacgaa cggcataacg aactgagata ttttccacag gccggaacca
    11881 attttcttgc ctctgccaga ttaaaatttt aagaatttca agttttttgt ctgaaaaatt
    11941 aaagagccgt cctgaacaac cggctcttta agaattttga gaatttggaa cattactttt
    12001 taatgatagt ctgatggtaa atctttccat ttgaatgcaa ttcaagaaaa taagttcctg
    12061 aaacataagt tttcattggt aaacaaatat cggattatta accatctttt aaaggaaaca
    12121 actgttattt aaaccaatta agaaaacaca tcttttggag gcagtgcttt taattaccgc
    12181 ctacttcaaa tttataaatg caaacatgtt gatactgttg acccggatta agaaccgttg
    12241 aaggaaagtt cgggtgattc ggactgtcgg ggaaatgttg ggtttccata gcaaatgaag
    12301 tgcgatacgt atagacttcg ccatatttac ctctttctgt tccattcaag aaattgcctc
    12361 cgtaaaactg aactcccggt tggtcggtaa tcactcgcat ttgaatgccg ttggaaggtt
    12421 cgtgaacaac ggcagcttct gtcagtttat ctcctttttt attcaatacc caattgtggt
    12481 cgtatccttt tccgttgatt aaatcgataa agttttcatt tactctttct cctattggcg
    12541 ttggtactgt aaaatccatt ggcgttccct ttaccggagc aatgattccg gttggaatca
    12601 atcctttgtc cgtcggtgta taattagacg catttatttc cagcacatgt gaattgatcg
    12661 atttcgcaat tccatccgaa aatccatgca agttaaaaaa catgtgattg gtcagattga
    12721 taatggttgg cttgtcggtc gtggcagaat attcaatctt cagctcgttg tcgtttgtca
    12781 atgtataaac caccttaacc gtcaaattac ccggataacc ttcttcgcca tcacgagaaa
    12841 agtatttcag ttccaaagca tcttcattgt tggcattttt gaatggtcgg gcatcccata
    12901 ttaccgtatg aaatcctttg ggtccaccgt gcaaatggtt ttccccatcg ttgatcggga
    12961 gctgataagt ctttccattc aattcaaatc ggcctttggc aattctgttg ccatatcttc
    13021 caattaatgc tccgaaataa acttcagtgg attgttggaa tcctttcagc gaggaatatc
    13081 cggttatcac atccttgaaa tcgtattttt tgtccggcac ccaaagaga
//
LOCUS       DAOSCH010000057        12687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3330_length_12687_cov_5.928515, whole genome shotgun sequence.
ACCESSION   DAOSCH010000057 DAOSCH010000000
VERSION     DAOSCH010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12687
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3330_length_12687_cov_5.928515"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..2131)
                     /locus_tag="PLZ54_05630"
                     /pseudo
     CDS             complement(1..2131)
                     /locus_tag="PLZ54_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272962.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
     gene            complement(2252..2761)
                     /locus_tag="PLZ54_05635"
     CDS             complement(2252..2761)
                     /locus_tag="PLZ54_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ66442.1"
                     /translation="METEYFPIVNEKGEIIGKESREKCHSGSFLLHPVVHLHVLNPNN
                     EIYLQKRAENKDVQPGKWDTSVGGHVDYGETIEEVLKREAFEELGIQKFEPEFLYQYI
                     YTSDVESELVYSYFTFYDGIISPSKDEISEGRFWKDEEIEQLMKKGIFTPNFEYEWKK
                     WKEWNPNHK"
     gene            3116..4153
                     /gene="asnA"
                     /locus_tag="PLZ54_05640"
     CDS             3116..4153
                     /gene="asnA"
                     /locus_tag="PLZ54_05640"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HOJ66443.1"
                     /translation="MSYFIRPKNYQPLLDLQQTELGIKKIKDFFQSNLSSELRLRRVT
                     APLFVLKDTGINDDLNGVERAVSFPIKDMNDATAEVVHSLAKWKRVALANYGIQNGYG
                     IYTDMNAIRADEELGNLHSLYVDQWDWERVMSREERNLEFLKEIVRRIYATFLRTEYL
                     VWESFPQIEPCLPSDIVFIHAEELRQMYPELTPKERENEFARIHKAIFVIGIGGKLGD
                     GSKHDGRAPDYDDWSTIAENGLPGLNGDIILWNPVLEMAFEVSSMGIRVDSDALLRQL
                     EMEGKQERLQLYFHKSLMEGRLPLSIGGGIGQSRLCMFFLRKAHIGEIQSSIWPEEIQ
                     QECKKLNIVLM"
     gene            4370..4963
                     /gene="hisIE"
                     /locus_tag="PLZ54_05645"
     CDS             4370..4963
                     /gene="hisIE"
                     /locus_tag="PLZ54_05645"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HOJ66444.1"
                     /translation="MNLDFQKMGGLIPAIIQDEWTSKVLMLGFMNEEALEKTLATGKV
                     TFFSRTKNRLWTKGEESGNFLNLVSITPDCDNDTLLVKVHPTGPVCHTGNDTCWGETN
                     QGDFSFINYLQNFIKKRYEEMPEGSYTTSLFKSGVNRMAQKIGEEAVETVIEAVNGTD
                     EGFIYEASDLIYHLIVLLTSKGFSLDDIGKELKKRHK"
     gene            4973..5647
                     /locus_tag="PLZ54_05650"
     CDS             4973..5647
                     /locus_tag="PLZ54_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ66445.1"
                     /translation="MTKKLLVKYVDVNLYQQEMLVLKDVQLEIYSGEFIYLLGKVGSG
                     KSSLLKSFYGEVPIDSGSAGVLNYDLRNLKSNEIPFLRRKIGIVFQDFQLLTDRTVHD
                     NLEFVLKATGWKDKQAIDEQIEAALLQVGMQNKSYKMPHQLSGGEQQRVVIARALLNS
                     PEMILADEPTGNLDPETGNELVQLLYEICRSSDTTVVMATHNMTWVEKYPGRIIRFEN
                     EQLIEN"
     gene            complement(5652..7133)
                     /gene="proS"
                     /locus_tag="PLZ54_05655"
     CDS             complement(5652..7133)
                     /gene="proS"
                     /locus_tag="PLZ54_05655"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOJ66446.1"
                     /translation="MGKELKELTPRSENYSQWYNDLVIKADLAENSSVRGCMVIKPYG
                     YAIWEKIQSELDRKFKETGHVNAYFPLFIPKSYLSREAEHVEGFAKECAVVTHHRLKA
                     DPEGNGLIVDPEAKLEEELIVRPTSETIIWSTYKNWIQSYRDLPILINQWANVVRWEM
                     RTRLFLRTAEFLWQEGHTAHATEAEALEEAKKMLNVYADFVENFMAIPVVKGVKSPNE
                     RFAGALETFCIEALMQDGKALQAGTSHFLGQNFAKAFDVTFVDKNNKQDYVWATSWGV
                     STRLIGALIMAHSDDNGLVLPPTLAPHQVVIVPIYKQPEQLSAITEKAEKIMKELKKF
                     GITVKYDDDDTKKPGWKFAEYELKGVPVRLAIGARDLENNTVEVARRDTLTKETVSAE
                     NIEIYVSKLLEEIQKNIYQKAFDFRTSVTREVNSYEEFKIEIEKGGFLLCHWDGTPET
                     EEKIKQETKATIRCIPLEGDQTPGVCMVTGKPSKQRVIFARAY"
     gene            complement(7260..7670)
                     /locus_tag="PLZ54_05660"
     CDS             complement(7260..7670)
                     /locus_tag="PLZ54_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOJ66447.1"
                     /translation="MIFQTEITLQAYRKGCHLITREILQHLPVLPEKGLLHLFLKHTS
                     AALTINENADPDVQTDLFEIFSRLVPENQSYYEHTLEGSDDMPAHAKSTLSGVSLLIP
                     ITNGHLNLGIWQGIYLCEFKNRGGNRTLVATIIS"
     gene            7963..9255
                     /gene="tyrS"
                     /locus_tag="PLZ54_05665"
     CDS             7963..9255
                     /gene="tyrS"
                     /locus_tag="PLZ54_05665"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010991893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOJ66448.1"
                     /translation="MNFIEELRWRGMIHSMMPGTEEQLAREMTTAYVGIDPTADSLHI
                     GHLVSIMILRHFQRAGHKPIALIGGATGMIGDPSMKSSERNLLDEATLRHNQACIKNQ
                     LTKFLDFGSDAPNAAELVNNYDWMKDYTFLNFIRDIGKHITVNYMMAKDSVKKRLSSD
                     SSEGMSFTEFSYQLIQGYDFLWLYENKNCKLQMGGSDQWGNITTGTELIRRKTGGEAF
                     ALVCPLITKADGGKFGKTESGNVWLDARYTSPYKFYQFWMNVSDADAEKYIKIFTFLS
                     KEEIDALIDEHTKAPHLRILQKKLAQEVTIMVHSEADYQAAVEASNILFGNSTPEALR
                     KLDEDTLLAVFEGVPQFNIPKSSLESGIKAVDLFTETAPVFPSKGEMRKMVQSGGVSI
                     NKNKLKQFDEVINTSYLIDNKYLLVQKGKKNYFLLIAE"
     gene            9570..9640
                     /locus_tag="PLZ54_05670"
     tRNA            9570..9640
                     /locus_tag="PLZ54_05670"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9602..9604,aa:Gln,seq:ctg)
     gene            9881..10582
                     /gene="radC"
                     /locus_tag="PLZ54_05675"
     CDS             9881..10582
                     /gene="radC"
                     /locus_tag="PLZ54_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOJ66449.1"
                     /translation="MEDNDKRLTLREWAEDDRPREKMLRKGIQSLSDAELLAILIRSG
                     SKNETVVELSRRILHECNDNLNELALLSVHDFCQRFKGIGEAKAITVIAALELGKRRK
                     TSEMLERKKITSSKDLLELFEPLLIDLPHEEFWVAFMNSGNKVLETYRLTQGGSKQTV
                     VDVPMLLKMALTKSAHALAVAHNHPSGQNQPSHEDEAITKKIKMGCDAVGITFLDHII
                     VARGKYYSFFDNGKF"
     gene            10649..11569
                     /locus_tag="PLZ54_05680"
     CDS             10649..11569
                     /locus_tag="PLZ54_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ66450.1"
                     /translation="MPFLEVKNVVKQYAEHLALNNVSLDVPENTIYGLLGPNGAGKTT
                     LIRIINCMTAPDSGTVLLNGKPLTFEDVRHIGYLPEERGLYKKMKVGEQALYFAQLKG
                     MSRHDALKALKYWFQKFEIQSWWNKKVEELSKGMAQKVQFIITILHEPKLLIFDEPFS
                     GFDPVNTDLLKQEILHLKEQGATIIFSTHNMASVEELCENISLIHRSKIVLQGNVAEI
                     RASFSNKTFKVITREPVLDGHYFELVHQTRKNGGVESIIKKVEACNNNILLSEILQQT
                     EILAFEELLPSMNDIFIETVSGHSKNSIEN"
     gene            11606..>12687
                     /locus_tag="PLZ54_05685"
     CDS             11606..>12687
                     /locus_tag="PLZ54_05685"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ66451.1"
                     /translation="MSKIHLIIQREYTTRVMKRSFILLTFLTPLFFAAMIILPVWMST
                     LKDEKEKHILVIDQTHQYINALKSNNTYIFDFIDQPLNQVRKTHKKDKKIAAILLITD
                     DLLKNPAAATLYSEEQVNLETKSYIEGLLNEYLREQKLASYNIPDLDKIIQDSNSKIN
                     ISTIRWDEEGKEKQSSAELALIIGMISAFIIYMFILTYGSQVMSGVIQEKTNRVVEII
                     VSSVKPFELMLGKIIGIALVGLTQFFLWIILTFVLLFVAQFFFSNQIDMSMLQQTNQM
                     AAMPAASMENMGNTNILVVLQSFDFVQIGIYFLIYFLGGYLLYASLFAAVGSAVDNET
                     DTQQFSFPLTLPIIFALFVAIYGAQNP"
BASE COUNT         3744 a         2399 c         2459 g         4085 t
ORIGIN      
        1 aaacctttca tttcttcttt tgtagctttc ttcagactaa tggctgcacc accaccggga
       61 gcaagttttt gttttaatat gctttttgaa ttgaccagaa acttttgaat tttataagcc
      121 atcggattct ctttccagtc ggcatttgct gcatcaccat aaattatagc aacataccat
      181 tgttttgcat ctaaaaagtt aaaaggaata acggccattc gtgcattttc atccgtaatg
      241 gcacccacaa accagtcatc ggtgttttta gcttttcgcg ctaccgtgat gtaatcgccc
      301 ggttcggctt caatgtattt tgtgtcgtcc caatcgacag ccacatcttt gataaattga
      361 aaagcatcca ttcttttttc gtaattttcg ggtaaatcgg ctgccatttg tagtggactg
      421 tacattgtta cataaagagc caattgtttt gccagtgttg tatgcacttg acagggattg
      481 cccggctgat aataactcat ttttatttca aaaattccgg gtgtataatc cattgggcct
      541 cccattaaac gggtaaaagg aagaatagtt tcatgttcgg gcggattgcc ggcactccaa
      601 gcattaaatt catttcctct ggctgcctcg caggctaacc agttgggata ggttcggtgc
      661 aatccggttg gtcgtacagg ttcgtgagca tcaagcataa ttttgttttc tgccattttt
      721 tcggctactc tgatatagtg attcaccatc cattgtccat gtgatattca ccacgaggaa
      781 taattctgcc cacataaccg gtttttactg catcgtaacc gttttctttc ataaattcta
      841 tggctttatc caatcgccgt tcataattgg ttaccgaggc cgaagtttcg tgatgcataa
      901 taattttaac tcctttggaa gcagcgtatc gatgaatttc cttcacatca aaatctggat
      961 agggagtaac aaaatcaaaa acttcttctt tccagtttcc catccaatct tcccaaccca
     1021 cattccatcc ttcgaccaac acacttggta tgccgttggc tgctgcaaaa tcaacatacc
     1081 tttttacgtt ttcggttttg gctccgtgtt gtggaagggg ttttaaagca ttccaatcgg
     1141 tgtctttcag ttttatgttt ggctcatgag cataattcca cgaccattta ttgggaacat
     1201 gcatggccca ccaaatccca ataaactttt gcggttttat aaaatcggta tctttaattt
     1261 tacaaggttc attcagattc aaaatcattt ttgaagccag tatatcggtt gctttatcgc
     1321 tgacaataat ggtacgccag ggcgtattct ggggagtctg cataaaagct tttactccta
     1381 cagcactggg aacaagcgaa gaagtcaata ccagcttttt ttgatcgata agcagctgca
     1441 tggccggata atcgaccaat gcagcttcaa aaatattgat atacaatccg ttatctgttt
     1501 tcatcatcat aggactttgc acggcgtttt tggcaaaata tccgtgtgtt cctattccag
     1561 atccatcaac ttttgaagcg tcaacttccg aaagttttgt cgtcgtataa taatattcgt
     1621 tggtatcata gtcgcccggt atccaaaatg ccttgtggtc agccgtcaac gcaaattgtg
     1681 taaattcctc cataatttga aaataactca aattctgctg tttgtcgaat tcgtatcgga
     1741 atcccaatcc atcgtcaaac aaacgaaaac gaattttcat ggttcgttga gtggactttt
     1801 gttcgagaca aaccaacatt tcgttgtaat gatttctgat ttgacgaact tctccccaaa
     1861 ttggttccca tgtttggtca aaagtgaggg tgtctatttt atgaatttga aaaccattta
     1921 cgaaagacgg catatttttt agttcaaaac ccatttttga cggtagaatt atcggttttc
     1981 ccctgtagaa gaaactgtat tcgggttcac cggatggcgt caattgaaac tcgagcttca
     2041 aatttttgtt gggagaaagt agtgtagctg catttaatgt gcagacaatc gaaaaaataa
     2101 tcagtgttga aagcaaaatt ttctttttca tgtgttgaat aaatttaaaa aaataattgt
     2161 atgaataaac aaaattaacg gttttttttg aagatttatc cattaggaat taaaatttta
     2221 caaacatcga ttgcaaacgt ttgaagaaaa gttatttgtg atttgggttc cattctttcc
     2281 actttttcca ttcatattca aaatttggtg taaatatgcc tttcttcatc agttgttcaa
     2341 tttcttcatc tttccaaaat cgtccttcac ttatctcatc tttcgatggg gaaataattc
     2401 cgtcgtaaaa agtgaaataa ctgtaaacta attccgactc aacatccgaa gtgtaaatat
     2461 attgataaag aaattcaggc tcaaattttt ggatgcccaa ttcttcaaag gcttctcttt
     2521 tcaatacctc ttctattgtt tctccataat ccacatgtcc gcctaccgaa gtgtcccatt
     2581 tcccgggttg cacgtctttg ttttcagctc ttttttggag gtaaatttca ttattgggat
     2641 ttaaaacgtg cagatggaca accggatgca ataaaaaaga accgctatga catttttccc
     2701 tgctttcctt cccgataatt tctccttttt cgttcactat gggaaaatat tcagtttcca
     2761 tgtctcaaaa tctttataag attaaaatag ttttcaaagt taatcaaaat tttttaaact
     2821 ttgttcgtaa aatcgcaaat tgtatatcta ttttttatca atactatgct tttactgtac
     2881 cattgtaaat aaaaatgtcg ataattgata tattttgatt ttttaacatc tcaatttggt
     2941 taaaaatttt attttcaatt tactgatttt caatggatta ttatttgttc cgaaaatttt
     3001 tactcttcct ttccttcttg tgaatcaaaa aaatcggtaa ggaatgatta tctttgcttc
     3061 ttaatttttt aaaacaatag aacattttat cgaccatcag caaataaatt aaaatatgag
     3121 ctattttatt cgaccaaaaa attatcaacc tttactcgat ttgcagcaaa ccgaattggg
     3181 catcaagaaa ataaaagatt tttttcagtc gaatctctcc tccgaattgc gtttgcgccg
     3241 tgtaactgca cctctttttg tcctcaagga tacgggaatt aatgacgatt taaacggtgt
     3301 ggaacgcgct gtaagttttc ccatcaaaga tatgaacgat gccactgccg aagttgttca
     3361 ttcgttggca aaatggaaaa gggtggcgtt ggccaattac ggtatccaaa acggatacgg
     3421 tatttacacc gatatgaatg ccattcgggc agacgaagag cttggaaatc tccactcttt
     3481 atatgtcgat cagtgggact gggaaagagt catgagccgc gaagaaagaa atttggaatt
     3541 tctaaaagag attgtccgtc gtatttatgc cacttttttg cggacagaat atctggtttg
     3601 ggaaagtttt cctcaaattg aaccttgttt gccttccgat atagtattta ttcatgcgga
     3661 agagcttcgg caaatgtacc ctgaacttac tccaaaggaa agagaaaacg agtttgccag
     3721 aatacacaaa gctatttttg tcattggcat agggggcaag ttgggcgatg gaagcaaaca
     3781 tgatggacgt gctcccgatt atgatgactg gtcgacgatt gccgaaaatg gcttgcccgg
     3841 acttaatggc gatattattc tttggaatcc ggttctcgaa atggcttttg aagtttcatc
     3901 aatgggcatt cgagtggaca gcgacgcatt attgcgtcag ttggaaatgg aaggaaagca
     3961 ggaaagattg caattgtatt tccacaaatc actcatggaa gggcgtctgc ctctttccat
     4021 tggcggaggt atcggacagt cgcgactttg tatgtttttc cttcgtaaag cgcatattgg
     4081 cgaaattcag tcaagtatct ggcccgaaga aattcaacag gaatgtaaaa aactaaatat
     4141 tgttttaatg taatcggttt ccaattattt caaccaaata aaattttccc agaataccct
     4201 ctggtttttg ggattttttg tgccaataaa tattgggaag cgaataaatt tttaacgaca
     4261 aatgaagcga aattcttaca aataatcttt tttgccgatt tttctattga atatgggcat
     4321 ttttttgtac tttcgcttgc tgtaaattca aaataaaatt tttgacaata tgaatttaga
     4381 ttttcaaaaa atgggcggac tgattccggc cattattcaa gatgagtgga cttccaaagt
     4441 cttgatgctg ggatttatga atgaagaagc actcgaaaaa actttggcta caggaaaagt
     4501 tacttttttc agccgaacca aaaatcgttt gtggacaaaa ggagaggaaa gcgggaattt
     4561 tttaaacctt gtttccatta cgcccgattg cgacaacgat actttgctcg tcaaagttca
     4621 tcccaccggc cctgtttgcc atacgggcaa tgacacttgt tggggagaaa ccaatcaggg
     4681 agattttagt tttataaatt atttgcagaa ttttatcaag aaaagatacg aagaaatgcc
     4741 cgaaggttct tatacgactt ctctatttaa aagtggtgtg aaccgaatgg ctcaaaaaat
     4801 tggcgaggaa gctgtcgaaa cagtgattga agcagttaac ggaacggatg aaggttttat
     4861 ttatgaagct tccgatttaa tttatcatct tattgttttg ctgacttcaa agggattttc
     4921 gctcgacgat attggaaaag aattgaaaaa acgacacaaa taagtgaaaa tgatgacaaa
     4981 aaaattattg gtcaaatatg tcgatgtaaa tctctatcag caggaaatgt tggtgttgaa
     5041 ggatgtgcag ctcgaaattt attccggcga atttatttat ttgttgggaa aagttggaag
     5101 tgggaaaagt tctttgctga aatcgtttta tggagaagta cctatcgatt caggttcagc
     5161 aggagtttta aactatgatt taagaaattt aaaatctaat gaaattcctt ttttgaggcg
     5221 aaaaatcggc atcgtttttc aggattttca actgctcacc gatcgaaccg tacacgataa
     5281 ccttgaattt gtattgaagg ctaccggttg gaaggacaaa caggccatag atgaacagat
     5341 tgaagcggct ttgttgcagg ttgggatgca gaacaaaagt tacaaaatgc ctcatcaact
     5401 ttccggcggc gaacagcaac gtgtagttat tgctcgggct ttactcaatt cacccgaaat
     5461 gattttggct gatgaaccga caggtaatct cgatcccgaa accggcaatg aattggttca
     5521 gctgctttac gaaatttgtc gctcgtcaga taccacagta gtaatggcca cccacaacat
     5581 gacttgggta gaaaaatatc ccggacgtat tatacgtttc gaaaacgaac aattgataga
     5641 aaactgattt ttcagtaagc tcgggcaaaa atcactcttt gtttggatgg tttgcctgtt
     5701 accatacaca ctcccggagt ttggtctcct tccagaggaa tacaccgaat ggtagctttg
     5761 gtttcttgct tgattttttc ttcggtttcc ggtgtgccat cccaatgaca aagtaaaaat
     5821 ccaccttttt ctatttcaat cttaaattct tcgtacgaat ttacttctct ggtaaccgaa
     5881 gttctgaagt caaaagcttt ttgataaata tttttctgaa tttcttccag aagtttggaa
     5941 acatatatct caatattttc ggccgaaaca gtttcctttg ttaaagtatc ccgacgtgcc
     6001 acttcaaccg tattgttttc caaatcacgt gcgccgatag ccaatcttac tggaacgcct
     6061 ttcagttcgt attcggcaaa tttccaaccc ggttttttgg tatcgtcatc atcatatttc
     6121 acggtaattc cgaacttttt caactccttc ataatttttt ctgccttttc ggtaattgca
     6181 gatagctgtt cgggttgttt gtaaatagga acaatgacta cttgatgtgg tgccagcgtt
     6241 ggtggcaaaa ccagcccgtt gtcgtcggag tgagccatga taagcgctcc gataagtctg
     6301 gttgaaactc cccaagaagt tgcccataca taatcctgct tgttgttttt atcgacaaat
     6361 gtaacatcaa atgcttttgc aaaattttgt cccagaaagt gggaagtacc tgcttgaaga
     6421 gctttcccat cttgcataag tgcctcaata caaaatgttt ccaaagctcc ggcaaaacgt
     6481 tcattgggcg atttcacgcc tttcaccact ggaattgcca tgaaattttc aacaaagtcg
     6541 gcataaacat tcaacatttt ttttgcttct tccagtgctt ctgcttccgt tgcatgagct
     6601 gtatgacctt cttgccacaa aaactcagcg gtacgcaaaa acaatcttgt gcgcatttcc
     6661 cagcgtacca catttgccca ttgattgatc aatatcggca aatcacggta cgattgaatc
     6721 cagtttttgt aagtactcca aattatggtt tcggaagtgg gtcttacaat cagttcttct
     6781 tccagcttag cttcaggatc cacaatcagt ccattccctt caggatcagc tttcagacga
     6841 tggtgcgtaa ccactgcaca ttctttggca aaaccttcca cgtgttcggc ttcccgactc
     6901 aaataggatt tgggaataaa cagcggaaaa taagcattga catgccctgt ttctttgaat
     6961 tttctgtcga gttccgattg tatcttttcc caaatagcat agccgtaggg tttaataacc
     7021 atacaacctc ttactgaaga attttctgcc aaatcggctt ttatcaccaa atcattgtac
     7081 cactgtgagt agttttccga acggggtgtc aattctttaa gttcttttcc cataatttgc
     7141 gtataatgat atttcttttt gaaaaggaaa cgatctgtaa tttgtaggat tgtttcccga
     7201 aaaatttcgg agtgcaaagt taatcaaaat ttacgactta tttgcttgct gaatcaggtt
     7261 taactgatta tggtggctac caaggtacga tttccgcctc tgtttttgaa ttcgcaaaga
     7321 taaattccct gccatattcc aagattcaaa tgtccattgg taatggggat aagcagactt
     7381 actccggaaa gggtgctttt ggcatgtgca ggcatatcat cgctgccttc cagtgtatgt
     7441 tcgtagtaag attgattttc cggcaccagt cggctaaaaa tttcaaacag atccgtttgt
     7501 acatcaggat cggcattttc gtttatggtt aaggcggcag aagtatgttt cagaaaaaga
     7561 tgcagcaatc ctttttcggg caggacaggc aaatgttgta aaatttcgcg tgtaattaaa
     7621 tggcagccct ttcggtaagc ttgaagtgtt atctcagttt gaaatatcat tgtttgaata
     7681 tttttcgaca aaaataactc ttcttaaaaa tttgtgcaat aaaccgacaa agagaaaatt
     7741 tgattacttg atttcgtcaa cacatttttt tataaaaagg gaagtggttg tagtcgttgc
     7801 gcattaaatt gtcgttccag aaaatcaaat caatatcaat atcaataact caaagtaaga
     7861 ttttatttta ccattgtttt ttttgagttt tcataggtct ttgggtaaat tgaaaataaa
     7921 cttgtaaatt tgcactccga attaaaaaac ctgaatttgc acatgaattt tattgaagaa
     7981 ttacgttggc ggggaatgat tcattccatg atgcccggca cggaagaaca acttgccaga
     8041 gaaatgacta cagcttatgt gggtatcgat ccaacagccg attcgctcca tattggtcat
     8101 ttggtcagta ttatgatttt gcgtcatttt caacgtgcag gccataaacc catcgctctg
     8161 ataggtggcg ctacaggaat gattggagat ccttccatga aatcatccga acgaaatctg
     8221 ttggacgaag ctaccttgcg acacaatcag gcgtgcatta agaatcagtt gacaaaattt
     8281 ctcgatttcg gcagcgatgc gcccaatgcc gctgaattgg tcaacaatta cgattggatg
     8341 aaagattata cctttcttaa ttttattcgc gatattggca agcacatcac cgttaattac
     8401 atgatggcga aagattccgt aaaaaaacgt ttaagcagcg attcgagcga aggaatgtct
     8461 tttaccgaat tttcctatca gttgattcag ggttacgatt ttttgtggct ttacgaaaat
     8521 aaaaattgta aacttcagat gggaggttcc gatcaatggg gaaacattac taccggaacc
     8581 gaactgattc gccgaaaaac tggtggcgaa gccttcgctt tggtttgccc gctgattacc
     8641 aaagccgatg gtggcaaatt tggtaaaacg gaaagcggaa atgtttggct tgatgctcgt
     8701 tatacttctc cttacaaatt ttatcagttt tggatgaatg taagcgatgc cgatgccgag
     8761 aaatatatta aaatatttac ttttctgagt aaggaagaaa tcgatgcact gatcgacgaa
     8821 cataccaaag cgcctcattt gcgaatttta caaaaaaaac tggcgcaaga agttaccatc
     8881 atggtacatt ctgaggctga ttatcaggct gccgttgaag cttccaatat tttatttgga
     8941 aattctacgc ctgaagcttt gagaaaactc gacgaggata cattgctggc cgtttttgaa
     9001 ggagttccgc aattcaacat tccgaaatct tctctcgaaa gcggaataaa ggcggtcgat
     9061 ctttttacgg aaaccgcacc cgtttttcct tcaaagggag aaatgagaaa aatggtacaa
     9121 tcgggaggag taagcataaa taaaaataaa ctgaaacagt ttgacgaagt tatcaacact
     9181 tcttacctta tcgacaataa atatttactg gttcagaaag ggaagaaaaa ttattttctg
     9241 ctgatagccg aatagaaaat cgcactactt caacatattt tggctgtaaa attttgacag
     9301 aaaaaaaatc ggttgtatct ttgtgcccgc ttgctgaaag attgcagcga aagtttgttt
     9361 gtctcatggt gtaatggtag cactgcagtt tttggtactg cctgtctagg ttcgaatcct
     9421 agtgagacaa ctgtttttct ggcggttgag ctttttcaaa aggttcaatc gtttttttta
     9481 caagccaaaa aaactctttg ccacaaacaa aataatttgt atttttgcaa agggttttaa
     9541 agttcccgat ttctctgaaa tacacattct gtctcatggt gtaatggtag cacgtcagat
     9601 tctggatctg cctgtctagg ttcgaatcct agtgagacaa ctacttccca acctgatttc
     9661 tccttttttt ctgtatcgat tcacaagata atctttccgt attgtttgca agtttactaa
     9721 actgatggta tttttatgcc cgaaaaaatt tatgaacctt tttattgttc aaaacaattt
     9781 attccgacaa taaaagtatt ttcgtaaatc cataaccaat cgtcctgctt taatttttta
     9841 ttcctttttt catagatttg tttgcaaaac attttataaa atggaagaca atgacaagcg
     9901 tttgacactt cgggaatggg cagaagatga tcgcccacgt gaaaaaatgc tgcggaaagg
     9961 tattcaaagt ctttccgatg ccgaattgct ggctattctc attcgctcgg gcagcaaaaa
    10021 tgaaaccgtc gttgaacttt ctcgccgtat tttgcatgaa tgtaacgata atttgaatga
    10081 attggcgctg ttgagtgttc atgatttttg tcagcgtttt aagggaattg gcgaagcaaa
    10141 agccattacg gtcattgctg ctcttgaact gggcaaacgc cgaaaaacca gtgaaatgtt
    10201 ggaacgtaag aaaattactt ccagcaagga tttactggaa ctttttgaac ctttactgat
    10261 tgatttgcca cacgaagaat tttgggttgc atttatgaac agtgggaaca aagttttgga
    10321 aacttatcgt ctgacgcaag gtggcagcaa gcaaacagtt gtggatgttc ccatgttgct
    10381 gaaaatggca cttacaaaat cggctcatgc tcttgctgtg gcacacaatc atccttcggg
    10441 acaaaatcaa cccagccatg aagacgaagc tattaccaaa aaaatcaaga tgggatgcga
    10501 tgcggttgga attacttttt tagatcacat catcgttgct cgtggaaaat actacagttt
    10561 ttttgataat ggaaaattct aagtcatttt gccgaaaaat gaaaattatt tcttctaaaa
    10621 atctataaaa acaattttgt aaagaattat gccttttttg gaagttaaaa atgttgtcaa
    10681 acaatatgca gagcatctgg ctttaaataa tgtgagtctg gacgttcccg aaaataccat
    10741 ttatggctta cttggcccga acggtgcagg aaaaaccaca ctgatacgta tcattaactg
    10801 catgactgct cccgatagtg gaacagtgct tttgaacgga aaacctctga cgtttgaaga
    10861 tgtgcgtcat attggatatt tgcctgaaga acgcggactt tacaagaaaa tgaaagttgg
    10921 tgaacaggcg ctttattttg cccaactgaa aggaatgtcg cgacacgatg cccttaaagc
    10981 tttaaaatac tggtttcaaa aatttgaaat tcaatcgtgg tggaacaaaa aagtggaaga
    11041 actttccaaa ggaatggcac agaaagttca attcattatc accattcttc acgaacccaa
    11101 attgctgatt tttgacgaac cattcagcgg attcgatccg gtgaacaccg atttgctgaa
    11161 acaggaaatt cttcacttga aagaacaggg agccaccatt attttttcta cgcacaatat
    11221 ggcttcagtg gaagaacttt gtgaaaatat ttcactcatt catcggtcga aaattgtttt
    11281 gcagggaaat gttgccgaaa ttcgggcttc gttttctaac aaaactttca aagtgataac
    11341 cagagaaccc gttttggatg gtcattattt tgaattggta catcaaactc ggaaaaacgg
    11401 tggggtagaa agtatcataa aaaaagtgga ggcttgcaat aacaacatcc ttttgtccga
    11461 aattttacaa caaaccgaaa ttttggcttt tgaagaatta ttgcccagca tgaacgatat
    11521 ttttatagaa accgtcagtg ggcactcgaa aaattcaatt gagaattagt gtcataataa
    11581 ctttcaaatc aaacaaattt tcagcatgtc taagattcat ctcattattc aaagagaata
    11641 tactacccgg gtgatgaaac gttcatttat tttgttgacg tttttaactc ccttgttttt
    11701 tgctgccatg attattctgc ctgtttggat gtccaccttg aaggatgaaa aggaaaaaca
    11761 tattttggtt attgatcaaa cccatcaata catcaatgcc ttgaaaagta acaatacata
    11821 tatttttgat ttcatcgatc aaccactcaa tcaagtgcgt aagacacata aaaaggacaa
    11881 aaaaattgct gccattttac tgattaccga tgatttgttg aaaaatccgg ctgccgcaac
    11941 actttactcc gaagaacaag tgaatctgga aacaaaaagt tacattgaag gtttgctgaa
    12001 tgaatatctt cgcgaacaga aactggcttc gtacaatatt ccggatttgg ataaaataat
    12061 tcaggattcc aattctaaga taaacatttc gaccattcgt tgggatgagg aagggaagga
    12121 aaaacaaagt tcggccgaat tggcgctgat tatcggaatg atttctgcct tcatcattta
    12181 catgtttatt cttacctacg gttcgcaagt gatgagcggc gttattcagg agaaaaccaa
    12241 tcgtgtggta gaaattattg tttcttcggt caaacctttt gaactcatgt tgggaaaaat
    12301 tattggcatt gcattagttg gcctcacaca attttttctt tggattattc ttactttcgt
    12361 tttgctgttt gtagctcaat ttttcttttc caatcaaatc gacatgagta tgcttcagca
    12421 gacaaatcag atggctgcca tgcctgctgc ttcaatggaa aatatgggta acacaaatat
    12481 attggtagtt ctccaaagtt tcgatttcgt acaaatcggc atatattttc ttatctattt
    12541 tctgggtggc tatttgcttt atgcttcgct tttcgctgct gttggttcgg cagtggacaa
    12601 cgaaaccgat acgcaacaat ttagtttccc gttgactttg cctattattt ttgctttgtt
    12661 tgtagccatt tatggagctc aaaatcc
//
LOCUS       DAOSCH010000058        12645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3348_length_12645_cov_6.661477, whole genome shotgun sequence.
ACCESSION   DAOSCH010000058 DAOSCH010000000
VERSION     DAOSCH010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12645
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3348_length_12645_cov_6.661477"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="PLZ54_05690"
     CDS             complement(<1..1394)
                     /locus_tag="PLZ54_05690"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ66452.1"
                     /translation="MKKNLVLRSVAIVLATFCSSLLFAQSAIEGKILDKKTKEPLVGA
                     SILIEGTSRGTTTDIDGNFQLKGLQAGKYTLSVSYVSYKKQRLPDIKVQNNEPTFMEI
                     EMEEAELMLQSVQVVAQHRTDTELSLLKSVRTAVQVVNGISSQQIGKTLDRDAAEVVK
                     RIPGVTVQDDRFVVVRGLNQRYNNVWLNNASTPSSETDVKAFSFDAVPSNMIDNIMIY
                     KTGSPELSAEATGGFVKIFTKNIPEENFQQIEYSFGYNDQTTFKDSYHLPMQTLDVFG
                     LGSLQRDLPVDFPENLNSLSIQQRDAAALKLSNHWVAQPSTALPNQKLTFSIGRKWNL
                     KEGNKIGNITSLSYSNSSSSRFSMENNMYERFDPVTETPIYLYNYAADIYSTDFKIGL
                     MHNWAYLTPNGTKIEFKNLLNQIGVDKTSNTFGWNNYRQGNFKYLSNQYSARTTYSGQ
                     LSGNHKLKNNEDNKL"
     gene            1861..3501
                     /locus_tag="PLZ54_05695"
     CDS             1861..3501
                     /locus_tag="PLZ54_05695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HOJ66453.1"
                     /translation="MKNFTQFGKFLLFLFFFFLVLLLIQAKATDFYFYVQFADKNNSP
                     YRLENPQEFLSERAIERRNFFSLSVDSTDLPVNPSYLSQVMNLGGEIHSQSRWLNGAT
                     VVMQDSALIEQVVSLPFVKWVQYTGKKATVSNVPKQPSKFSPAVDYGLSGSQIAQVKG
                     NFLHELGYFGKNIVVGVLDAGFLNVDINVAFDSLRLHNRLLGTKDFVGGSDLYRGNFH
                     GGNVLSIMAGNLPQNYLGTAPDASYWLIRTEDVDTEYLKELDFWVAGIEFADSVGSDV
                     VNSSLGYFTFDDPAMNFTYADMNGEVSRAAKAASMAAQKGMIVVCSAGNEGDKTWKYI
                     STPADAKGIITVGSVTADGTPSIFSSFGPSADGRVKPEICARGTQTSLINSSGNIVAG
                     NGTSYSSPLICGLMACYLQYFKEQHKLISIPQLLQSVFVTGSLYSSPTDQMGYGIPDL
                     QLAFLSTAFNGLNNQTQNQPQWLVDEKNKTIQLNFAGKTDNSAISVQIHAMDGKLLLS
                     RFYRSSSIVLSIENLSSGIYLVSVENGGKIFSKKILVP"
     gene            3556..4803
                     /gene="xseA"
                     /locus_tag="PLZ54_05700"
     CDS             3556..4803
                     /gene="xseA"
                     /locus_tag="PLZ54_05700"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOJ66454.1"
                     /translation="MNENQNMTSLSLSELAAAIEETIRLNFDRPIWMRAEISELRENS
                     NGHCYMELIEKDENSDNLLAKVKATCWASVYRLLKPYFENATGQTFRAGLKVLLSVTV
                     EFHPVYGLSLTVRDIEPTFTIGEMAARRMQIIRRLEADGIADMNKQIPLPRLVQRLAV
                     VSSPTAAGYGDFCDQLKNNSGKFVFYTRLFPALMQGEQAESSIIAALEKIFRHAELFD
                     AVVIIRGGGATTDLSCFDSYELAVNCAQFPLPIITGIGHQRDVSILDLVAHSSLKTPT
                     AVAEFLIDRLQEAENLVKYAASDIYSFSKNYLDASLREINEKKLLIRQVLRNSLMRKQ
                     NEINRRISRLKTAVRMKLLREQNRLTLMEKSIETHSPAFMLKYGYSFTTLHGKKILSV
                     HEVKPGDRVQTYLHDGSFESELI"
     gene            4849..5217
                     /gene="folB"
                     /locus_tag="PLZ54_05705"
     CDS             4849..5217
                     /gene="folB"
                     /locus_tag="PLZ54_05705"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOJ66455.1"
                     /translation="MAFIYLENMEFYSFHGCLEEERVKGNTFLVSVCMEVDTEKAGKT
                     DELSDTVNYQQVYDIVKKEMEIPSHLIEHVAQRIYDRIFEQIPQISGLQIRLSKLNPP
                     LGGKVEKASIEISGKRSDHC"
     gene            complement(5224..7143)
                     /locus_tag="PLZ54_05710"
     CDS             complement(5224..7143)
                     /locus_tag="PLZ54_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ66456.1"
                     /translation="MKKILLITGIICGCNVFRFSGQVTTDTLSVQLSDVEINATRTKL
                     YPEMGRILTVIDKNEIERSAVRSIDELLDYVSGLDIRQRGRNGVQADISVRGGSFDQA
                     LILLNGINITDPQTGHFNLDIPIQLSDVSKVEILKGSSARMLGPNAFSGAINIITEKG
                     EKPFLKAGLEAGSFGYGEQSVTTAFIQKPYTLLSLSHKSSDGYTNNTDFENSNIFLHS
                     KLNSPKFGNIQFQAALQKKGFGANGFYSLAYPHQFESTQTLMAGLRWELIRRNISYHA
                     QSYWRQHHDRFELFRNFEGAPSSYTQHNYHLTDVVGGKFDASVANSWGKITFGIEGRN
                     EHIFSTVLGNEMNKTKPDPYENGINFNHSANRLLGTAFIDYAKIFDRWYVSAGGALSH
                     ATAFGTNPYGGFDIAFHPNNMTKIFFSSNTAMRLPTFTDLYYRSPTQISNPNLKPEHS
                     TTWELGAGIQQKHWQADATTFYRKGKNIIDWVKEPDSTKWESRNLTEVNAVGLDMNLN
                     YSFSNSFLQKAGLSYSFVQMDKNAENFDSKYALDYMKHKLIATLQHKTVKNCSLNWKL
                     SFLDRAGNYTDFYTQQPVNYQPYFLLDMKGLWEVEKYAVYLDLNNIFDKTYADYGGIV
                     QPGFNFTAGIKWKFK"
     gene            7791..9053
                     /locus_tag="PLZ54_05715"
     CDS             7791..9053
                     /locus_tag="PLZ54_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HOJ66457.1"
                     /translation="MFKFLKQIDNKSYRPVFGALDIFKFIGPGLLVTVGFIDPGNWAS
                     NLAAGSNYGYSLLWMVTLSTIMLIMLQHNVAHLGIASGLCLSEAASIYLKPAYSKFVL
                     LSAMMASVSTSLAEILGGSIALNMLFDIPVRAGALLVSAFVLIMLFTNSYKVIEKWII
                     AFVSIIGLSFLYELSLVNIDWNHAVVGWVTPSFPNGSMLIIMSVLGAVVMPHNLFLHS
                     EVIQSRQWNLTDDKTIKRQLKYEFLDTIISMLIGWAINSAMILLAAATFFETKTPVTE
                     LEQANHLLAPLLGKNASVVFAVALLFSGISSSITSGMAGGTIFAGMYKEPYDIKDNHS
                     RLGVLLSLLLAAGIIFLISNPFQGLIVSQMLLSIQLPFTIFTQVHLTSSEKVMGKYKN
                     TTFSKILLYLLGVIVSVLNILLFISFFK"
     gene            complement(9066..9650)
                     /locus_tag="PLZ54_05720"
     CDS             complement(9066..9650)
                     /locus_tag="PLZ54_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOJ66458.1"
                     /translation="MLSHLEKYQIILASKSSRRQDLFKGLNIPFEIYPIEVDESFPPE
                     LIGMDIPMYLSKKKAEACTDIMKENTMLLTADTIVWYQGQVFGKPADRNAAKFMLTTL
                     SGKTHDVITSVSITTMKKQRTFGVASEVRFARLAPEEIDFYLDNFQPYDKAGAYGVQE
                     WIGFVGVEYINGSYYNVMGLPMQRLYNELKNWKE"
     gene            9784..11190
                     /locus_tag="PLZ54_05725"
     CDS             9784..11190
                     /locus_tag="PLZ54_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ66459.1"
                     /translation="MLSNYFVSLIKAQLPFEPNEQQNDLLEALGRFLLSSEKRKIFLL
                     KGYAGTGKTSIVGALVRALNKIGQKTVLLAPTGRAAKVFANYSGTPAFTIHKKIYRQK
                     SLTDFHFQLAENLFSDVLFIVDEASMISNTDFDSVFGSGNLLDDLMEYVFSGRNCSLM
                     LLGDSAQLPPVGQAESPALDVDILKQYRLNVFEFTLTHVVRQALKSGILHNASLIREK
                     LTNKNLVRFPPFQLEKFADISRLSPAELTDEIQKEYAGAGVEDTMVITRSNYRANYYN
                     NGIRSRVMMKEEELSNGDLLMVTRNNYFWPKYYPEIDFIANGDILEVLRIRKFTEMYG
                     FRFVDATVRSLDYDWEIDTRIWLDTLNAETPAKAQQMNKILFEKVAEDYSEIKQKRQK
                     VKKIYENEFFNALQVKFAYAITCHKAQGGQWKKVFVDPGRISDEQINDDFYRWLYTAL
                     TRATENVFLVNFPMKFFS"
     gene            11425..>12645
                     /locus_tag="PLZ54_05730"
     CDS             11425..>12645
                     /locus_tag="PLZ54_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66460.1"
                     /translation="MKSSASHFLVAIFLSVLILPSCMNDVDLTNISDKVQWKGSLAVP
                     IGEAALTLEDLLAQIDSQNILVTESQEILYQDSDSIVSRFRDVDLAQYSQSLVKSYYV
                     SPIVRTIPANTIFPTLNDNVIIDLGINNSPSAERVDSAVIYSANLSVKVNVENININP
                     DNLKLTLEFPTDKVRTKNGSPIKIEFTPGAFNHPENVTIRDFLINTSGNASGIPVTLK
                     LDITTGSSPVTVGPSSVVGVELKFTGFNYKVAYGYFTPAVIATKILTQDLDLPSFTDD
                     GSFLFANPKVFLTVKSNIGAYLKFNVDYVKAYPKSDSSQVVYASFNGSRSTAESLDNK
                     PAVPGLWIAKKLRTLDKDFGATDLLFSNENKPDVLEYKYSVDIDGSKNDGKPDFITPD
                     GNIKVLYKIQIPLYL"
BASE COUNT         3565 a         2343 c         2549 g         4188 t
ORIGIN      
        1 agtttgttgt cttcgttgtt tttcagctta tggttcccgc tcaactggcc ggaataggtt
       61 gtacgtgcag aatactgatt gctgagatat ttaaaatttc cttgacggta attgttccat
      121 ccgaatgtat tagaagtttt atccactcca atctgattca aaagattttt aaattcgatc
      181 tttgttccat tcggtgttag gtaagcccaa ttgtgcatca gtccgatttt aaaatcggta
      241 ctgtagatgt cggccgcata attataaaga taaatcggag tttcggtgac ggggtcgaag
      301 cgttcataca tgttgttctc catactgaag cgggaagatg aggaattact gtagctaaga
      361 gaagtgatgt tgccaatttt atttccttct ttcaaattcc atttcctgcc gatagaaaaa
      421 gtcaattttt gatttggcaa tgctgtagag ggctgtgcaa cccagtgatt gctcaatttc
      481 aaggctgctg catctcgctg ttgaatagag agggagttca agttttcggg gaaatcgacg
      541 ggtaaatctc gttgcaggct acctaaacca aaaacgtcca atgtttgcat cggtaggtga
      601 taactgtctt taaaggtagt ctggtcattg tatccaaaac tgtattcgat ttgttgaaaa
      661 ttttcttccg gaatattttt ggtaaagatt tttacaaatc ctcctgttgc ttcagctgaa
      721 agttcaggag aaccggtttt gtatatcatt atgttgtcaa tcatattgct cggaacagca
      781 tcgaacgaaa atgcctttac gtcggtttcg ctgcttggcg tggaagcatt gttgagccat
      841 acattattgt atcgttgatt gagaccgcgt acaacgacaa atcggtcatc ctgcacggtt
      901 acacccggaa ttcgctttac cacttcggca gcatcccgat cgagtgtttt accaatttgc
      961 tgggaagaaa tgccattcac tacctgtacg gccgtgcgga cactctttaa caaggataat
     1021 tccgtatcgg tgcgatgttg tgctactacc tgcacacttt gaagcattaa ttcggcttct
     1081 tccatttcga tttccatgaa tgttggttcg ttattctgca cttttatgtc gggcaaccgt
     1141 tgctttttgt aagaaacgta agaaacagat aaagtatatt ttccggcttg cagccctttt
     1201 aattggaaat ttccgtcgat gtcggtagtt gttccccgcg aggttccttc aatgagaata
     1261 ctggcaccta ccagtggttc tttggttttt ttgtccaaaa tttttccctc tatcgcactt
     1321 tgtgcaaaga gaagcgaaga gcaaaatgtt gctaaaacta ttgcaacaga gcgtaatact
     1381 aaattttttt tcatctgatt tttcttctga atttttatgc tgcaaaatta attcacaact
     1441 gtttcaaaat tgataaaatg atattacaat gcggttataa tgattttttg aaagaatatt
     1501 gaaatacctt tttctatttt aacatgagtt tgagataagt atattttata agcaatttta
     1561 aataagctat aaaggttgtt gaaaagtaag tattcacatt ttatctaagg ttaattgttt
     1621 ataaataagg tttttatttt cagaaagtga accttatttt tttaaataaa aaccttagta
     1681 acgataatca acaacaggaa taaaccttat taccttatta attatcaatt tttttgtatg
     1741 attaaaacat gtaatcgttc tatcgaactt acattattaa ttttttttgc aaagaagcac
     1801 tttcaaaatt cgttttattg aattattccg gtatttttgt acataaaaaa tccgtaacat
     1861 atgaaaaatt ttacccaatt tggcaaattc cttctttttt tgtttttttt ctttttggta
     1921 cttctattga ttcaggcaaa ggcaaccgat ttctatttct atgtgcagtt tgccgataaa
     1981 aataattctc cttatcgatt ggaaaatccg caagaatttc tttctgaacg cgcaattgag
     2041 cgacgcaatt tcttttcgct ttccgttgat tcaaccgatt tgccggttaa tccttcctat
     2101 ctctcacaag tgatgaatct gggtggcgaa attcattctc aaagccgatg gttgaatgga
     2161 gcaactgtgg tcatgcagga ttctgcactg attgagcaag tagtttccct tccgtttgta
     2221 aaatgggttc aatacaccgg aaaaaaagct acggtttcga atgttccgaa acagccttca
     2281 aagttttctc cggccgtcga ctatggactt tccggttctc aaatagctca ggtaaaagga
     2341 aattttcttc acgaattggg atattttggg aaaaatattg tggttggggt gctggatgcc
     2401 ggatttctta atgtagatat caatgttgct ttcgacagcc ttcggttgca taaccgattg
     2461 cttggaacga aagattttgt tggtggttca gatctgtatc gtggaaattt tcacggagga
     2521 aatgtgcttt cgattatggc aggaaattta cctcaaaatt atttgggaac agctcccgat
     2581 gcttcttatt ggcttatcag gaccgaagat gttgacaccg aatacctgaa agaactcgat
     2641 ttttgggttg ccggaatcga gtttgccgac agcgtcggct cggatgttgt caattcgtct
     2701 ttgggttatt ttacctttga tgatccggcc atgaatttta cttatgccga tatgaacgga
     2761 gaggtttcgc gtgcggctaa agctgcttca atggctgctc aaaaaggtat gatagtagtt
     2821 tgtagtgccg gcaacgaagg cgacaaaact tggaaatata tttctacacc ggccgatgcc
     2881 aaaggcatta ttacggttgg aagcgttaca gccgatggga cacccagcat cttttcttca
     2941 tttggcccat ccgccgacgg aagagtaaaa cccgaaattt gtgcacgcgg tacccaaacc
     3001 agtcttataa attcgagtgg caacattgta gccggtaacg gaacttccta ttcttctcca
     3061 cttatttgcg gattgatggc ttgttacctt caatatttca aagagcagca taaactgatt
     3121 tcgataccgc aacttctaca gtcggttttc gtaaccggaa gcttgtatag ttcgccaacc
     3181 gatcagatgg gatatggcat acccgattta caactggctt ttcttagtac agcgttcaac
     3241 ggattgaaca accaaaccca aaatcaacca caatggttgg tggatgaaaa gaacaaaacc
     3301 attcagctta attttgcggg aaaaaccgat aattcagcta taagcgttca aattcatgca
     3361 atggatggaa agttgttgct cagcagattt tatcgttctt cttccattgt cctttccatt
     3421 gaaaatctga gttcaggtat ttatcttgtt tcggtagaaa atggcgggaa gattttttca
     3481 aagaaaattc ttgttcccta aatttgtcaa gaaaaatatt tctgttgtat ctttgaagtg
     3541 aaaaattctc aatgaatgaa cgaaaaccaa aatatgactt ctctttcctt atctgaactg
     3601 gctgctgcta ttgaagaaac cattcgtctg aattttgatc gccccatatg gatgcgtgcc
     3661 gaaatcagtg aattgcgtga aaattccaat ggacactgtt acatggaatt aatagaaaag
     3721 gatgagaatt ccgacaattt gctggcaaaa gtcaaagcca catgttgggc ttcggtgtat
     3781 cgattgttga aaccttactt tgaaaatgct accggtcaga catttcgcgc tggattgaag
     3841 gttttgttgt ccgttacggt agaatttcat ccggtttatg gtttgagcct tacggtacgc
     3901 gacattgaac cgacgtttac cattggcgaa atggcagcgc gcagaatgca gattattcgt
     3961 cgtttggaag ctgatggcat tgccgacatg aacaaacaga ttcctttacc gcgattggtt
     4021 cagcgtctgg ctgttgtttc ttcacccact gctgccggtt atggagactt ttgcgatcag
     4081 ctgaaaaaca attccggtaa atttgtgttt tatacccgcc ttttccctgc cttaatgcag
     4141 ggcgagcaag ccgaaagctc cattatcgca gctttggaaa agatttttcg tcatgcggaa
     4201 ctttttgatg cagtggtgat tattcgaggt ggcggtgcaa caaccgattt gtcctgtttc
     4261 gattcctacg aattggcagt gaattgtgct cagtttccct tgcccatcat caccggcatc
     4321 ggccatcagc gtgatgtttc tattcttgat ttggtggcac attcgagttt gaaaacaccc
     4381 accgccgttg ccgaatttct catagatcgc ctgcaggaag ccgaaaatct ggttaaatat
     4441 gcagcttccg atatttattc tttttctaaa aattatctcg atgcttcact tagagaaatc
     4501 aacgaaaaaa agctgttgat tcgccaagtt ctgagaaaca gtttaatgcg caaacaaaat
     4561 gaaatcaatc gtcggataag tcgtttgaaa acggcagtac ggatgaaatt gctcagagaa
     4621 caaaatcgat tgactttaat ggaaaaatct attgaaactc attctccggc ctttatgttg
     4681 aaatatggtt acagtttcac tacccttcac ggaaagaaaa ttctttccgt tcatgaagtg
     4741 aaacccggcg accgtgttca aacttatctg catgatggga gttttgagag tgagttgatt
     4801 tgacgcgaaa aaataaaggg aatttattaa atactcaatt cttaacttat ggcattcatt
     4861 tatctcgaaa atatggaatt ttattccttt catggttgct tggaagaaga acgtgtaaaa
     4921 ggaaatactt ttctggtttc ggtttgtatg gaagttgata cggaaaaggc tggaaaaacg
     4981 gacgaacttt cggatacggt gaattatcag caggtgtacg atattgtaaa aaaagagatg
     5041 gaaattcctt cccatctcat agaacatgtc gctcaaagga tttacgaccg gatttttgaa
     5101 caaattccgc aaatttccgg gttgcagatc cgactttcca aattaaatcc tcctttgggt
     5161 ggaaaagtag aaaaagcttc gatagaaatt tccgggaaac gttctgatca ttgttgatcg
     5221 ttgttatttg aacttccatt ttatcccggc agtgaagtta aatcccggtt gtacaattcc
     5281 gccataatcc gcgtaagttt tgtcgaaaat attattcaga tcaaggtaaa ctgcatattt
     5341 ctctacttcc cataatcctt tcatatccag taaaaaataa ggttgataat ttacgggttg
     5401 ttgcgtataa aagtcggtgt agtttcctgc tcgatccaaa aaggagagtt tccagttgag
     5461 agaacagttt ttcactgttt tatgttgtaa agttgctatc agcttgtgtt tcatgtaatc
     5521 caacgcatat ttggaatcga aattttcggc atttttatcc atctgaacaa aagaatagga
     5581 aagcccggct ttttgaagga aagaatttga aaaagaatag ttcaaattca tatccagccc
     5641 cacagcgtta acttcagtca aatttcggct ttcccatttt gtcgagtcgg gttcctttac
     5701 ccagtcgata atgtttttcc cttttcggta gaaggtcgtt gcatcagctt gccagtgttt
     5761 ttgttgaatg ccggctccca attcccatgt tgtggaatgt tcaggtttta aattgggatt
     5821 gctgatttgc gttgggcttc ggtaatataa atcggtaaaa gttggtaacc tcatggccgt
     5881 atttgatgaa aaaaatatct tggtcatgtt gtttggatga aaagcaatgt caaatccgcc
     5941 atacggattc gttccgaaag cagtggcatg tgacaatgct cccccggccg aaacatacca
     6001 tctatcaaaa atttttgcat aatcaataaa tgccgtcccc agtaaacgat ttgcactatg
     6061 attaaaattt attccgtttt cgtaagggtc tggtttcgtt ttattcatct catttcccaa
     6121 aactgtgctg aaaatatgtt catttcttcc ttcaatacca aaagtaattt ttccccatga
     6181 atttgccacc gaagcatcaa attttcctcc cacaacatct gtcaggtgat aattgtgttg
     6241 tgtgtacgac gaaggagccc cttcaaagtt gcgaaacagt tcaaatcggt cgtgatgttg
     6301 tcgccaatag ctctgagcat ggtagctgat gtttcttcgg ataagttccc atctcaaccc
     6361 cgccatcagg gtttgcgtac tttcaaactg atgcggataa gccaacgaat aaaatccgtt
     6421 tgcaccaaaa cctttttttt gtaaagctgc ttgaaactgt atattaccaa atttcgggct
     6481 attcagtttc gagtgcagaa aaatgttgct attctcaaaa tctgtattgt tggtataacc
     6541 atcgctgctt ttgtgagata aagaaaggag cgtataaggt ttttggataa atgcggtagt
     6601 aaccgactgt tctccataac caaaacttcc ggcttccaaa cctgctttta aaaaaggttt
     6661 ttctcctttt tcagttatta tgttaatcgc accgctgaaa gcatttggcc ctaacattcg
     6721 ggctgaagaa ccttttaaaa tttcaacttt actcacatcg gagagttgaa tgggaatatc
     6781 aaggttaaaa tgtcccgttt gagggtcggt aatgttgatg ccattcagca aaattagcgc
     6841 ctggtcaaat gagcctccac gaaccgagat atcggcttga acgccatttc tgccacgttg
     6901 acgaatatcc agaccggaaa cataatcgag caattcatca atgctgcgta ctgccgagcg
     6961 ttcgatttca ttcttgtcga taacagttag gatacgaccc atttcgggat aaagttttgt
     7021 tcgggtggca ttgatttcaa cgtctgaaag ttgtaccgac aaggtgtcgg tggtaacttg
     7081 tcccgaaaaa cgaaaaacat tgcatccgca aataattcct gtgattaaaa gaattttctt
     7141 catttgaaaa aattttagtt aaacaatatt tatatgaatt cgatataaga atattttata
     7201 actaattata aataagccat taaaattgag gaaaaaagta tttacctgtt gttatggtta
     7261 gttgttctta aagtaataag aatcagcctt atcaattctc aactttttat atgattaata
     7321 caggtaatag ccgtatcgta cttacgtaat aattatgctt tgattcattt tttttccccg
     7381 atttgtttaa tatggcctac taacaaaaaa aatcgactga aagaaatgaa ttcctttcag
     7441 tcgacaacaa catatataag tacatgaaag acagcatttt acgtcttcag cagagaatat
     7501 aacaggagta ttgagttgaa tattttcaag aatttcctcc gctcggaatt tttcttcctt
     7561 gcactgaact aaagaattaa tggatgtagt ataattaaat catgtataat cacgtattgc
     7621 aaaaataact aaaaagccac ttctatttgt gatatatttt tatcaaacta tgatattaaa
     7681 tcatttgtaa ataaaattaa agcgtaaaat aaataattcg cggtaaatgg ttgtttaaac
     7741 aaaatttgat atttttgtcc agtttttcaa ccaataaaaa gaatcgaaac gtgttcaagt
     7801 ttttgaagca gatcgacaat aaatcttatc gtccggtatt tggggcattg gatattttta
     7861 aatttatcgg ccccggttta ctggtaacgg ttggttttat cgatccgggc aattgggctt
     7921 ctaatctggc tgccggttcg aattacgggt attcattgct gtggatggta actttgtcca
     7981 ccataatgct tattatgttg cagcacaatg tagcgcattt gggcattgca tccggtttgt
     8041 gcttgtccga agccgcttct atttacctga aaccagcgta ttccaagttt gtcctgcttt
     8101 cggccatgat ggcttctgtt tctacttctt tggccgaaat tttgggagga agcattgctt
     8161 tgaacatgct gttcgatatt cccgttcgag ccggtgcttt gctggtttct gcttttgtac
     8221 ttattatgct gtttaccaat tcttataagg ttatcgaaaa atggatcatt gcttttgtct
     8281 ccattattgg actttcattt ttgtatgaat tgtcgttggt aaatatagat tggaatcatg
     8341 ctgttgtggg ctgggtaact ccaagttttc caaatggctc catgttaatt attatgagcg
     8401 tgttgggagc agtggtcatg ccacacaatt tgtttttgca ttcggaagtg attcagagcc
     8461 gccaatggaa tttgaccgat gataaaacca tcaaacggca gttgaaatac gaatttcttg
     8521 atactataat ttccatgctt attggttggg ctatcaacag tgccatgatt ttgctggctg
     8581 ccgccacttt ttttgaaacg aaaacgccgg ttaccgaatt ggaacaagcc aatcatttgc
     8641 tggctccgtt gctgggtaaa aatgcttctg ttgtttttgc cgttgcattg ttgttttcag
     8701 gtatttcctc ttccattact tccggtatgg ccggaggaac aatttttgcc ggcatgtaca
     8761 aggaacctta cgatattaaa gacaaccatt cgcgactggg tgtcctttta tcacttttac
     8821 tggcagcagg tattatcttt ttgatttcca acccatttca agggcttatt gtctctcaaa
     8881 tgttattgag tatccaatta cctttcacta tttttacgca agtgcactta acttcatcag
     8941 aaaaagtaat ggggaaatat aaaaatacta ctttttcaaa aatactgctt tacttgttgg
     9001 gcgttattgt ttccgtactg aatattttac tgtttatcag ctttttcaag taagattttt
     9061 ggtttttatt ctttccagtt tttcagctca ttgtacaatc gttgcatggg tagacccatt
     9121 acgttgtagt aggatccgtt aatatattca actcctacaa agccaatcca ttcctgtact
     9181 ccataagcgc cggctttgtc gtagggttga aaattgtcga gataaaaatc aatctcttca
     9241 ggtgccaatc gtgcaaaacg aacttcagaa gccacgccaa aggttcgttg ttttttcatg
     9301 gtagtaatgc tgacgctggt gatgacatcg tgcgttttac ccgacagtgt ggttaacata
     9361 aatttagctg cgtttcggtc ggcgggtttt ccgaaaactt gtccctgata ccaaacaatg
     9421 gtatcggctg taagcagcat ggtattttct ttcataatgt cggtgcaagc ttccgctttt
     9481 ttcttcgata gatacatggg aatgtccatc cctataagtt cgggaggaaa agactcatct
     9541 acttcaatcg gataaatttc aaaagggata ttcaatcctt taaataaatc ctgtcttctt
     9601 gatgattttg aggcaagaat aatttggtat ttttccaaat gtgaaagcaa tttgaacatt
     9661 tttttcggtt tgaaggattg tttttgttct ggtaccgaat tactccggac attattgcta
     9721 caaaaatatg attttttttt gttgtgcagc atattttgca aaaatatcgt ttaaatttgc
     9781 cgtatgttaa gtaactattt tgtttcgctg ataaaagcgc agttgccttt tgaaccgaat
     9841 gaacagcaaa atgatttgct tgaagcattg ggaaggtttc tgctatccag tgaaaaaaga
     9901 aaaatttttc tgttgaaagg ttatgccgga accggtaaaa cttcgattgt gggtgctttg
     9961 gtcagggcgt taaataaaat tgggcaaaag acagttttac tggctccaac gggcagggcg
    10021 gcaaaagttt ttgcaaatta ttcgggaacg ccggctttta cgatacacaa gaaaatttat
    10081 cgtcaaaaat ccttgaccga tttccatttt cagttggcgg aaaatctttt ctcggatgta
    10141 ctttttattg tggatgaggc ttcgatgatt tccaataccg atttcgattc ggtttttggt
    10201 tcgggtaact tgttggacga tttgatggaa tatgttttta gcggacgcaa ctgcagcttg
    10261 atgctgttgg gcgattctgc ccagcttccg ccggtaggac aagccgaaag tccggcattg
    10321 gatgttgata tattgaaaca ataccgattg aatgtttttg aatttacatt gacccatgtc
    10381 gttcgtcagg cgctgaaaag tggcattttg cataatgctt ctttgataag ggaaaagctg
    10441 acaaataaaa atttagttcg atttcctcct tttcagttgg aaaaatttgc cgatatttcc
    10501 cgcttaagtc cggccgaatt gacggatgaa atacagaaag aatatgccgg cgcaggagtg
    10561 gaggatacga tggtcattac gcgttccaat tacagggcta attattacaa caatggcatt
    10621 cgctcgcggg tgatgatgaa agaagaggag ttgtcgaatg gtgacttgct gatggttacg
    10681 cgtaacaatt atttctggcc aaaatattat cccgaaatag attttatagc aaacggcgat
    10741 atactggaag ttttgcgtat cagaaaattt accgaaatgt atggttttcg atttgtggat
    10801 gccactgtgc gctctctcga ttatgattgg gaaattgata cccgcatttg gctggatacc
    10861 ctcaacgcag aaacaccggc aaaagcgcag caaatgaata agatattgtt tgaaaaagtg
    10921 gcggaagatt attctgaaat taagcaaaaa cggcagaaag tgaagaaaat ttatgaaaat
    10981 gaatttttca atgcccttca ggtgaaattt gcttatgcca ttacgtgtca taaagcgcag
    11041 ggcggtcaat ggaaaaaggt ttttgtcgat cccggaagga tttccgacga acaaatcaat
    11101 gacgattttt accgatggct ttatacggcc ttgacccgcg ccaccgaaaa tgtttttctg
    11161 gtaaattttc ccatgaaatt tttctcgtaa aaaacagaga atttttttgc attttccaac
    11221 ttttaattac ttttgtgaag aatataaaaa ttataaaatc ttttaaattt attctaaatt
    11281 tcatttttgc ttttgaggct gctgtactgt ttgtttgcgc tctttgtcaa cattctaaac
    11341 atttttttga tttaattttt ccctgttagc tgtttattat ttgattttac tttttcaatt
    11401 gtgtaacctt aatattcaat taaaatgaaa tcttcagctt cccattttct tgtggcaatc
    11461 tttttaagcg ttttgatttt gccctcatgc atgaacgatg tggatcttac caacatctcc
    11521 gataaggttc aatggaaggg ttctttggct gtacctattg gagaagccgc tctgacattg
    11581 gaagatttgc tggctcaaat cgacagtcag aatattcttg ttaccgaaag tcaagaaatt
    11641 ctttatcagg attccgacag tatagtttcc cgtttcaggg atgttgattt agctcagtat
    11701 tcccaatcac tggtaaaatc ttactatgtt tctccaatag ttagaactat tcctgctaac
    11761 acaattttcc caactttaaa tgataatgta attatcgatt tgggcataaa caactctcct
    11821 tccgctgaaa gagttgacag tgcagttatc tattcggcca atttgtcggt gaaggttaat
    11881 gtggaaaata tcaacattaa tcccgataat ctgaaactga cgctggagtt ccccactgac
    11941 aaggtgcgta ccaagaacgg ctccccgata aaaattgaat ttacacctgg agcttttaac
    12001 catcctgaaa atgtaaccat cagagatttt ttgattaata cctctggaaa tgcgtcggga
    12061 ataccggtaa ctttaaagtt ggatattacc accggcagtt ctcccgttac tgtaggacca
    12121 agttcggtag ttggcgtaga actgaaattt accggtttta actataaggt tgcttacggt
    12181 tattttacac cggcagtaat tgcaaccaaa atactgacac aagatttgga tttaccttct
    12241 ttcacggatg atggctcgtt tttgtttgcc aatcccaaag tttttcttac cgttaaaagc
    12301 aatatcgggg cttacctgaa atttaatgtg gattatgtga aagcctatcc caaatccgat
    12361 tcttctcaag tggtttatgc atctttcaat ggaagtcgtt caacagccga atcgttggat
    12421 aacaagccgg cagttccggg tttatggatt gctaaaaaac ttagaacttt agacaaggat
    12481 tttggtgcta ccgatttgct tttcagtaat gaaaacaaac ccgatgtatt ggaatataaa
    12541 tacagcgttg acattgacgg aagcaaaaat gatggtaaac ctgattttat aacgcccgac
    12601 ggtaacatta aagttttgta taagattcag attccattat atctc
//
LOCUS       DAOSCH010000059        12447 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3432_length_12447_cov_6.729987, whole genome shotgun sequence.
ACCESSION   DAOSCH010000059 DAOSCH010000000
VERSION     DAOSCH010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12447
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3432_length_12447_cov_6.729987"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1341
                     /gene="holB"
                     /locus_tag="PLZ54_05735"
     CDS             190..1341
                     /gene="holB"
                     /locus_tag="PLZ54_05735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOJ66461.1"
                     /translation="MLFSQVIGQEEVKKRLIRTVKEQKIPHAQLFYGPEGIGKLPLAI
                     AYAQYICCEHPLENDACGVCPSCVQFGKLAHPDLHFVFPIIKSEKKKLLVCDDFMDSF
                     RQIFAERIYFSQNDWYQKISGEAKQGMIYTSEGDEIIRKVNLMTYESRYKVMLIWLPE
                     RMNDQCANKLLKILEEPPENTVFLLVSNSPEEIIATILSRTQQVKIPRLSNEEIAEAL
                     VSRYQISENEAANAAQISEGSFLKALALIDENNVNRENFEHFVNIMRLAWIIGNKGDY
                     ASLKKIRQWSEEMAAASVGREGQKKFLSYAQRMIRENFIFNLHQPELNFMTSFEKEFS
                     VGFAPFINETNAEDMMNEFALAERHIEQNVNSKMVFFDLALKITKLLKK"
     gene            1445..2662
                     /gene="ricT"
                     /locus_tag="PLZ54_05740"
     CDS             1445..2662
                     /gene="ricT"
                     /locus_tag="PLZ54_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005647784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOJ66462.1"
                     /translation="MDYRLDIGGYQIKKKSCNRCGNLKLSVHDWMADLPESQKETDFV
                     EVQFKNTRKGYYLNSTKIPLEKGDIVAVESSPGHDIGEVTLVGKLVLLQMKKNNVHPE
                     KMEIRRIYRKAKDTDLQKYQEAKNREHATMIQARQIAERMNLKMKIGDVEFQGDGNKA
                     IFYYIADERVDFRELIKVFAETFHVRIEMKQIGARQEAGRIGGIGPCGRELCCASWMT
                     CFNSVATSSARCQDISLNPQKLAGQCSKLKCCMNYELDTYQEAMKEFPSKEIQLETLD
                     NTYYHFKTDVFKGQITYSTAQNFGANIITISVQRAKEVIAMNKKGQKPEKLEEISEET
                     SQPRVVQDFENVVGQESVTRFDERKRKNPRFKNKNKKQKNNSDSNGQTASENKIAKPV
                     KKNEQNKENNNNK"
     gene            2628..3119
                     /locus_tag="PLZ54_05745"
     CDS             2628..3119
                     /locus_tag="PLZ54_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOJ66463.1"
                     /translation="MNKTKKITTTSKFVLLLSITCLMVSCNRNDVYLQYRSLPTKGWN
                     KDSVLTFDVPITDTTSLYNVYVNIRHRGEYPYQNLWFFLRQATPLMDTLQKDTIECYL
                     ADQRGKWLGSGVGSVYEMSILYRQKVSFPRAGIYRYQIIQGMRDSILAGINDVGIKIE
                     KVK"
     gene            3137..3898
                     /gene="trmB"
                     /locus_tag="PLZ54_05750"
     CDS             3137..3898
                     /gene="trmB"
                     /locus_tag="PLZ54_05750"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOJ66464.1"
                     /translation="MSKNKLAKFAEMETFPHVFQFPAKMLRNEHSNFEMQGKWNEKFF
                     GNSNPIVVELGCGKGEYTVGLAEIFPEKNFIGVDIKGARMWTGAKYSFEKKIPNVAFL
                     RTRIEMLHYFFGENEVSEIWLTFPDPQMKKITKRLTSSRFLKLYSRFTVENALIHLKT
                     DSNFLFTYTMELLKLNHFPIEFSTDNLYEIASDHPILSIKTFYEQQWIERGIPIKYIE
                     FKLEKRIEILEPEVEIEHDQYKSFGRRPKHETTKN"
     gene            3920..5038
                     /locus_tag="PLZ54_05755"
     CDS             3920..5038
                     /locus_tag="PLZ54_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOJ66465.1"
                     /translation="MNLYPKLILETLSHVRYPGKGQDIVSLGMVQDNIRIEGNKVSFS
                     LIFEKPNDPFVKSLVKAAEQAVLTYVGKEVDIKGNITAVFKKEIPTAKENSILPGVSN
                     IIAVFSGKGGVGKSTIAANLAVALASLGYRVGLLDADIHGPSVPKMFGLEDARPFLEE
                     VDGKELIVPIEKYGIKLLSIGFFVDPDNALVWRGSMASNALKQLITDAHWGELDYFIM
                     DLPPGTGDIHLTLVQTMGITGAIVVTTPQEVALADARKGINMFTNEKVNVPVLGLVEN
                     MAWFTPAELPENKYYIFGKEGGKRLAEEFNIPLLGQIPIVQSIRESGDAGKPIATEED
                     SILSVSFRQLAENVVAAVEQRNKIMEKTKKVEINHPRK"
     gene            5160..5822
                     /locus_tag="PLZ54_05760"
     CDS             5160..5822
                     /locus_tag="PLZ54_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOJ66466.1"
                     /translation="MKKSLKPVFILTGILYFYLPLSAQTYRIEAGYLNQVRSGSGLSS
                     TYFDGAQVGVSSDFALKNNFSYSVGGYYSFLYSFKEQLFPHDTYVRYTTYSHSLDIPV
                     RLNYTLTIKKVKLFAYAGPNFNIGLYQPQKTVSTLTPELTELVQEFQHVTIPNDTTRS
                     ELYNDNVLRRLNIQLGAGGGIQWRKYRLKSGYDFGLLDLYKAKTASLHQSGWFVSLGY
                     EF"
     gene            5963..6595
                     /locus_tag="PLZ54_05765"
     CDS             5963..6595
                     /locus_tag="PLZ54_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOJ66467.1"
                     /translation="MKRVKFFVFTAILFAAVTLNAQSFGLEAGYGNSSTFVGEGDTLN
                     TLNGFHIGPTAELQIQGPLSLQYGLFYNFLTSSKTYENSLVDTKISTTFHSIDIPVRL
                     AFTVPAGNNVGVFIFGGPSFNVGISKKSTGKVTSSLGTIETTIDYYKTDDEGNSRQSR
                     FDIQMGVGAGIKVNGVQLRVGYDWGLLDTNNSENYTLTRDEFKASIGFDL"
     gene            6752..8056
                     /gene="murF"
                     /locus_tag="PLZ54_05770"
     CDS             6752..8056
                     /gene="murF"
                     /locus_tag="PLZ54_05770"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOJ66468.1"
                     /translation="MQLYDIFLQHPIITTDSRNCPENSLFFALKGENFDANAFAHSAL
                     EKGCAFAVVDEKEYAVDNRFILVDDVLKTLQQLAHFHRQQMDIPVLGITGTNGKTTTK
                     ELITAVLREKFNILSTQGNLNNHIGVPLTLLQLKPEHQLVVIEMGANHPGEIDQLCQI
                     AAPNYGLITNVGKAHLEGFGSFEGVMRTKGELYDYVFRHGKKIFIDKNNNFLREMAFS
                     AGFSTSERIIEYGLNIPNKEPAAISGEILENSVFLKMNCHTPEGTFIVETRLIGAYNA
                     ENILAAIAVGSFFGVENNAIQRGIENYIPQNNRSQLTITQKNKLIVDAYNANPSSMSA
                     AIKNFARMEGEPKTLIIGDMLELGKQSEEEHRNIILLLQQNGFKNVLLVGSQFQKVND
                     NYRTFQNVEDLIKYLESSPLQGQNILIKGSRGIHLEKVLPFL"
     gene            8112..8999
                     /locus_tag="PLZ54_05775"
     CDS             8112..8999
                     /locus_tag="PLZ54_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66469.1"
                     /translation="MKKNNLILIVLSVVVLLLIGTVIFFVQVNKQKDAEMSEVVEMMN
                     FEKEQLEKDYSDLALEYDTYSSTIRNDSLVQLLQNEKMKVQQLLQELRITKATNARRI
                     AELRNELATLRTVMAQYVHQIDSLSAANKVLRVENVEVKRKYEAASETVKQLSKEKEN
                     LTEVVTRASMLEISNFSMTPLNSKNRKTGRYSQITTLQFNYTIQKNITCPPGMKTLYL
                     RLTRPDGEVMTKDPDDVFLYENKYIAYSAKKDFEYAGEALSDVIYWKVDEILQVGTYR
                     ADFFVDGFRIGSFTFEIKK"
     gene            9020..10393
                     /locus_tag="PLZ54_05780"
     CDS             9020..10393
                     /locus_tag="PLZ54_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HOJ66470.1"
                     /translation="MKPARKFHFDASKIAFDEVHRKKIDFNMSRYDLAVETGMKRYKD
                     VEAAKNQAAAIKHEVLSHLDEYLLQFEKNISSHGAEVLWARNSEEAIVYVEKILVEND
                     ARLLVKSKSMTTEEIELNAAAAKLGCESVETDLGEFIVQTAGEKPYHIVTPAMHKSKS
                     EIADLFHEKFDTPSDSTPEQLSDFVRRLLRKKFTQAEIGVTGANFLIADMGGISVTEN
                     EGNALMSTSFPKIHIVFAGIEKIIPKMEQLGLFYPLLAVHGTGQQMTAYNTIFTGPRQ
                     TGETDGPEKMYVILLDNGRIGIFADDEAYQSLACIRCGACLNGCPVYRTIGGYTYNTT
                     YSGPIGSAISPFLKGFRDFYHLSYASTLCGKCAEVCPVKIPLPDILLANRRKAVDQHL
                     SSALETAAMKGFRLIASKRKGFDFFPAGMKNSAFYPLNFWGWGPERTVPKFAKKSFSQ
                     LYRKNNK"
     gene            10506..10961
                     /locus_tag="PLZ54_05785"
     CDS             10506..10961
                     /locus_tag="PLZ54_05785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ66471.1"
                     /translation="MKPKSFFLFISTLLIFSFAGCKENNYEDWKIVNDQWMLKHQSDS
                     GFVVTSSGLCYQIIHQSVGRKPNASSWITANYKGYLIDGTLIEKGTYDYYLSSSISGW
                     QEGITKMRDGSTFIFYIPSSLGYGKEGSGSKIPPYSALIYEVTLVSSYY"
     gene            10990..12303
                     /locus_tag="PLZ54_05790"
     CDS             10990..12303
                     /locus_tag="PLZ54_05790"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOJ66472.1"
                     /translation="MKKIFILLLTSFAICSIWSQTYTVETIPNPKKLNRFAFVSNPDG
                     ILNPETESQINNLLDSLEKKTGAEVAIVLVNSIGNEDIKSFAEQLFNSWKIGKEKEDN
                     GLLVLFVMDQRKVTFEVGYGLEGILPDAICKRIQIESMIPEFKKGNYDAGILAGVQRI
                     YATILKEPVQVEKTAPVNWKEILPLALGFYLIWLVLTWLWMGNAMNKVKINPRLNSNM
                     ARYIAAKNERNGIVSVMAIFLPIAAFIAILVLSQAIYLLLLLPLPFMTIPPLIYANYR
                     LQKIRKEPFPCSVCGGQMHFVPEKEEDVYLKLSQQFEEQLHAVDYDVFVCDNCKNEAI
                     FTIDRPSAYSECPKCGTKAFILKDKKLTMAPTYFNAGTERLTYRCEFCGYEENKNRRI
                     PRLTRANSTFIGGAAAGSIFSGSGGFGGGSFGGGMSGGGGATSGW"
BASE COUNT         4064 a         2306 c         2523 g         3554 t
ORIGIN      
        1 attccttaaa attgactatc ctcgccgcaa gcatcgagga attcttacga ttaaaacatt
       61 ttcatctgtc tcaaggaacc tgaatttcat tctctacgaa taaagtcttt attttctttg
      121 ctggatttaa aatttcaaaa ttatctcgta aatttgcatg aatatagaga ataatttttc
      181 cgacaagtca tgctgttttc tcaagttatc ggacaagaag aagtaaaaaa gcgattgatt
      241 cgcacggtga aggaacagaa aattcctcat gcccaactat tctatggacc tgaagggatt
      301 ggaaaacttc cgttggcaat agcctatgct caatacatct gttgcgaaca tccgctcgaa
      361 aacgatgcgt gtggagtttg tccgtcttgt gttcaattcg gtaaactggc gcatcccgac
      421 ttgcattttg tcttccctat tatcaaatcg gagaaaaaaa aattactggt ttgtgatgat
      481 tttatggaca gtttcaggca aatatttgca gaacgtattt atttcagcca aaacgactgg
      541 taccagaaaa tttccggcga agccaagcaa ggaatgattt ataccagcga gggagacgaa
      601 attattcgaa aagttaacct gatgacttat gaatcaaggt acaaagttat gcttatctgg
      661 ttgcccgaga ggatgaacga ccaatgtgcc aataagcttc tgaagatact ggaagaaccg
      721 cccgaaaaca ccgtttttct tttggtttca aattctcccg aagaaattat tgccaccatt
      781 ttatctcgca cgcaacaggt taaaattccc cgcttaagca atgaggaaat tgctgaagca
      841 cttgttagcc gttatcagat ttctgaaaat gaagcggcca atgcagctca aatttcggaa
      901 ggaagttttc tgaaagcttt ggctctgata gacgagaaca acgtgaacag ggaaaatttt
      961 gaacattttg taaatattat gcgtttagca tggataattg gcaacaaagg cgattatgca
     1021 tcattgaaaa aaatccggca atggtccgaa gaaatggctg ccgcatcagt gggcagagaa
     1081 ggacagaaaa aatttttgag ttatgcacaa cgcatgattc gcgaaaattt cattttcaat
     1141 ctgcatcagc ccgaactgaa ttttatgact tcgtttgaaa aagaattttc agtcggattt
     1201 gctccattta tcaatgaaac caatgccgaa gacatgatga atgagtttgc gttagcagaa
     1261 cggcacatcg aacaaaacgt caattcaaaa atggtttttt ttgatttggc gttgaaaatc
     1321 actaaattgc ttaaaaaata aaatttatga acagacggaa aacctttaaa gaaataatcg
     1381 atcaaaataa aatctttttt tcatttgggt cttccggaaa ataaaatatt atcattcaaa
     1441 aattatggat tatcgattag atataggcgg ttaccaaata aaaaagaaaa gctgtaaccg
     1501 atgcggcaat ctgaaactta gcgttcacga ctggatggcc gatttgccgg agtcgcagaa
     1561 agaaaccgat tttgtcgaag tgcagtttaa aaacaccaga aaaggatact atctcaacag
     1621 taccaaaatt cctcttgaga aaggggacat agtggcagtc gaatcatcac cgggacacga
     1681 cattggcgaa gttacgctag tagggaagct ggtattgctg caaatgaaaa agaacaatgt
     1741 tcatcccgaa aagatggaaa tcaggcgaat ttatcgaaaa gccaaagata ccgaccttca
     1801 aaaatatcag gaagccaaaa acagggaaca cgctaccatg attcaggcca gacaaatagc
     1861 cgaaaggatg aacctgaaaa tgaaaattgg agatgtagaa tttcagggcg atggaaataa
     1921 ggccattttt tactacatcg ccgacgaacg ggtagatttc agggaactga taaaggtatt
     1981 tgcagaaact tttcacgtgc gcattgaaat gaaacagatt ggtgcccgtc aggaagcagg
     2041 gcgtatagga ggaattggcc cttgtggaag ggaattgtgc tgcgcttcgt ggatgacctg
     2101 tttcaattct gttgccacca gttcagcccg ctgccaggat atttcgctta atccacaaaa
     2161 actggccggc caatgcagca aattaaagtg ctgtatgaat tacgaactgg atacctacca
     2221 ggaagccatg aaagaatttc catccaaaga aattcagctc gaaacgcttg acaatactta
     2281 ttatcatttc aaaacagatg ttttcaaggg acaaataacc tattccactg ctcaaaattt
     2341 tggtgccaac attatcacta tttcagttca aagagctaaa gaagtgattg ccatgaacaa
     2401 aaaaggccag aaaccggaaa aacttgagga aatttcggag gaaacatcac aacctcgagt
     2461 agtacaggat tttgaaaatg ttgtaggtca ggaaagtgtt acccgtttcg acgagcgaaa
     2521 acggaaaaat ccacgcttca aaaataaaaa caaaaaacag aaaaataatt ccgactcaaa
     2581 cggacaaact gcctccgaaa ataaaattgc aaaacccgtc aaaaaaaatg aacaaaacaa
     2641 agaaaataac aacaacaagt aaatttgttt tgcttttgtc catcacctgt ttgatggttt
     2701 cctgcaaccg aaatgatgtt tatttgcagt atcgttcact gcccacaaaa ggttggaaca
     2761 aagattcggt gctgactttc gatgtcccca ttactgacac aacctctctt tataatgttt
     2821 acgtcaatat ccgtcatcga ggcgaatatc cgtatcagaa tctttggttt ttccttcgtc
     2881 aagctactcc tttgatggac accttgcaaa aagacaccat tgaatgctat cttgcagatc
     2941 agcgtggaaa atggctcggg tcgggagttg gttccgttta tgaaatgtcg attctttatc
     3001 gtcaaaaagt tagttttcct cgtgcgggaa tttatcgata ccaaattatt caagggatgc
     3061 gagattccat ccttgccggc attaacgatg tgggtattaa aatcgaaaaa gtcaagtaac
     3121 aagaaatttc ggtaccgtga gtaaaaacaa actggcaaaa tttgccgaaa tggaaacttt
     3181 tccacatgtt tttcagtttc ctgccaagat gctgaggaac gagcattcaa atttcgaaat
     3241 gcaggggaaa tggaacgaga aattttttgg caattcaaat cccattgttg tcgaattggg
     3301 atgcggtaaa ggagaatata ccgtcggctt ggctgaaatt tttcctgaaa aaaactttat
     3361 tggcgtcgat attaagggag cacgtatgtg gacgggagct aaatattcgt ttgaaaagaa
     3421 aatcccaaat gtagcttttt tgcgtacacg cattgaaatg ctccattact tttttggaga
     3481 gaatgaagtc agtgaaatat ggttgacttt ccccgatcca caaatgaaaa aaatcaccaa
     3541 aagacttact tcatcccgat ttctgaaact gtatagccgt ttcactgtcg aaaatgcttt
     3601 gattcatttg aaaactgaca gcaatttttt gtttacctat acgatggaac tgttgaaatt
     3661 gaaccatttc cctatagaat tttcaaccga caatttatat gaaattgcca gtgatcatcc
     3721 tatactcagc atcaaaacct tttatgaaca acagtggatc gaaagaggaa tccccataaa
     3781 atatatcgaa ttcaaactcg aaaaaagaat cgaaattctc gaacccgaag tagaaatcga
     3841 gcacgatcaa tataaaagtt ttggaagaag gccaaaacat gaaacaacta aaaattaaaa
     3901 attttagaac attaaaataa tgaatctata tcctaaatta attctggaaa ccctttcaca
     3961 cgtacgttat ccgggcaaag gtcaggatat tgtatctttg ggtatggtgc aggataatat
     4021 ccgcattgaa ggaaacaaag taagcttttc tctgattttt gaaaagccca acgatccgtt
     4081 tgtaaaatcg cttgttaaag cagctgaaca agctgtgttg acttatgtgg gaaaagaagt
     4141 ggacataaaa gggaacataa cagcagtttt caaaaaagaa attccaacag ccaaagaaaa
     4201 ctccattctt ccgggagtaa gtaatattat tgccgttttt tcaggcaagg gaggtgtggg
     4261 aaaatctacc attgctgcaa atcttgccgt agcgcttgct tcgctcggat acagggttgg
     4321 acttctcgat gccgatattc acgggccttc cgttcccaaa atgtttggat tggaagatgc
     4381 acggcctttt ttggaagaag ttgacgggaa agaactgatt gttcctatcg aaaaatacgg
     4441 cattaaactt ctgtccatcg gcttttttgt cgatcccgac aatgctttgg tatggcgtgg
     4501 ttcaatggca agtaatgccc tcaaacagct tatcaccgac gcacattggg gcgagctgga
     4561 ttattttatc atggatttgc cgcccggaac cggcgatatt catctcacac tggtacaaac
     4621 aatgggcatt acgggagcaa ttgtggtaac aacgccacaa gaggtagctt tggccgatgc
     4681 cagaaaaggc atcaatatgt ttaccaacga aaaagtgaac gttccggtac ttggcttggt
     4741 agaaaatatg gcatggttca cgcctgcaga acttccggaa aacaaatact atattttcgg
     4801 aaaagaagga ggaaaacgac tggctgaaga attcaacatc ccgttgctcg gacaaattcc
     4861 gatagtacaa agcattcggg aatcgggcga tgccgggaaa ccgattgcaa cagaagaaga
     4921 cagcatttta tcggtttctt ttcgacaact ggctgaaaat gtagtagctg ctgtcgaaca
     4981 aagaaataaa attatggaga aaaccaaaaa ggtggaaatc aatcatcccc gtaagtaata
     5041 cagaaaatgg cgttaacaag ataaagcata acttcattag ttttcacttt agggcttgtt
     5101 tctctttaaa ataaaaagac ttatctttgc ctcagaaaac tttttacaat tgcatataca
     5161 tgaaaaagtc tttaaaaccc gtatttattt tgaccgggat cttgtatttt tatcttcctc
     5221 tttccgccca gacatacaga atcgaagcag gctatttgaa tcaggtgcgc agtggttcag
     5281 ggttaagctc cacttatttt gatggtgcac aagtaggtgt atcatccgac tttgccctga
     5341 aaaacaattt cagttattct gtaggtggct attattcatt tctatattcc ttcaaggaac
     5401 agctttttcc acacgatacg tatgttcgct ataccacata ctcacattca ttggatattc
     5461 cggtcagact taactacacc ttgaccataa aaaaggtaaa attgtttgcc tatgccggac
     5521 caaattttaa tatcggatta tatcagcccc aaaaaaccgt ttctacatta acacccgaac
     5581 ttaccgaact tgttcaggaa tttcaacacg ttaccattcc taacgatact acccgttccg
     5641 aattgtataa cgataacgta ttgagaaggt tgaatattca actgggagcc ggaggcggta
     5701 ttcagtggag aaaatatcgt ttaaagagtg gttacgattt tggattgctc gatttgtaca
     5761 aagccaaaac tgcaagtctt caccaaagtg ggtggtttgt ttccttaggt tacgaatttt
     5821 agttaatcta ctgtttgcaa ataaattagg ttaaatttaa aatcagcttt tttacttgta
     5881 aaaacttctt tattacacgt tggtatattt tttgtttttg gttcttaact aaaattcagt
     5941 tatcaactta aaatcaaaaa gtatgaaaag agttaagttt tttgttttca cagcaatttt
     6001 gtttgctgct gttacgctca atgcgcaaag tttcggattg gaagccggtt atggaaattc
     6061 ttctactttt gtaggagaag gcgatacgct caacacatta aatggatttc atattgggcc
     6121 aacagccgaa ttgcagattc aagggccact gagtttgcag tatggattgt tctacaattt
     6181 tttaaccagc agcaaaacct acgaaaattc attggtcgat accaaaatct ccaccacctt
     6241 tcattcgata gatattccgg ttcgattagc gtttaccgtt cctgcaggca ataatgttgg
     6301 ggtatttatt tttggtggac caagttttaa tgtcggtatc agcaagaaat cgacaggaaa
     6361 ggttaccagt agtttaggta cgatagaaac aacaattgat tattacaaaa ctgacgacga
     6421 aggcaacagc cggcaatcgc gttttgatat tcaaatgggt gtaggagccg gtataaaagt
     6481 taatggcgtt caacttcgag tgggctacga ctggggattg ctggatacca acaacagtga
     6541 aaactacact ttgactcgcg acgaattcaa ggcttcaata ggatttgatc tctaaactgc
     6601 gagaaaaata aattttctac attctataaa aaggcttaac tctatgaatg agtttggcct
     6661 tttttgttaa aatgatttga atgtttccat tcattcagag gattatcact atttttgtga
     6721 tgaattagcg taaaaatttt tattttactt catgcaatta tacgatatat ttctacaaca
     6781 ccctattatt actaccgata gcagaaactg tccggaaaat tctctttttt tcgcattgaa
     6841 aggtgaaaat ttcgatgcca atgcttttgc tcattcagcg ttggaaaaag gttgtgcttt
     6901 cgctgtggtt gacgaaaaag aatatgctgt tgacaatcgt tttatattgg ttgatgatgt
     6961 actgaaaaca cttcagcaac tggcacactt tcaccgtcaa caaatggata ttcccgttct
     7021 gggaataacc ggcacaaacg ggaaaaccac tacaaaagag cttattactg cagtgttgag
     7081 agaaaagttc aatatccttt ctacgcaggg aaatctgaac aatcatattg gtgtgccatt
     7141 gactttattg caattgaagc ccgaacatca gttggtagtt attgaaatgg gagccaatca
     7201 tcccggtgag attgatcaat tatgccaaat cgcggctccg aattatgggc tgataacgaa
     7261 tgtcggaaaa gcacacttgg aaggttttgg gtcctttgaa ggcgtcatgc gcacaaaagg
     7321 cgaattgtat gactatgttt ttcggcatgg caaaaaaatt tttatcgaca aaaataataa
     7381 tttcctccgt gaaatggctt tcagtgcagg tttttcgacg agtgagagaa ttattgaata
     7441 tggtttgaac attcccaata aagagccggc tgcaatttcg ggagaaattt tggaaaattc
     7501 cgtttttctt aaaatgaatt gtcatacacc cgaaggcact tttattgttg aaacacgatt
     7561 gattggcgca tacaacgctg aaaatatttt ggctgccata gctgtaggtt cattctttgg
     7621 tgtagaaaac aatgccatac aacgaggcat tgaaaattat attccacaga ataatcggtc
     7681 gcaactgacc atcacgcaaa aaaacaaatt gattgtggat gcttacaacg caaatccttc
     7741 gagcatgagc gctgccataa agaattttgc tcggatggaa ggagaaccca agacgctgat
     7801 tattggcgac atgctcgaac tcggtaaaca aagcgaagaa gaacatcgga atattattct
     7861 acttcttcaa caaaatggtt ttaaaaacgt tttgctcgtt ggatctcagt ttcaaaaggt
     7921 caacgacaac tatcggactt ttcaaaacgt ggaagattta atcaaatatt tggaatcaag
     7981 cccgttgcaa ggacaaaata ttcttattaa gggttccaga ggaatacatc tggaaaaagt
     8041 tttgccattt ttataagttt atttcattta atgaatttct atcatttaac aacttaatta
     8101 tgtctatctg aatgaagaag aataatttga ttcttattgt actgtcggtt gtcgttttgc
     8161 tgttaatcgg tacagtaatt tttttcgtac aagtaaacaa acaaaaagat gctgaaatgt
     8221 ctgaagtagt ggaaatgatg aattttgaaa aggaacagct ggaaaaagat tattcagacc
     8281 ttgcattgga atacgacact tattcgtcaa ccattcggaa tgattcgttg gtgcaactgc
     8341 ttcagaatga aaagatgaaa gtgcagcagc ttttacagga gcttagaata acaaaagcaa
     8401 ccaacgcccg gagaattgcg gaattaagaa atgaattggc cactttgcgg acagtgatgg
     8461 cccagtacgt tcatcaaatc gattcgttga gtgcagccaa caaagtattg cgcgtagaaa
     8521 acgtggaagt gaaacgtaaa tacgaagctg ctagcgaaac agtgaaacag ttatccaaag
     8581 aaaaagaaaa tctgacagaa gtggtaactc gtgcttcaat gctggaaata tcaaatttca
     8641 gcatgactcc tttgaacagc aaaaatcgga aaacaggcag atattcacaa ataaccactt
     8701 tacagtttaa ttataccatt caaaagaaca ttacctgtcc gccgggaatg aaaacgcttt
     8761 atttgcgtct gaccaggccc gatggtgaag taatgacaaa agatccagat gacgtttttc
     8821 tctacgaaaa caaatatatt gcctattcgg caaaaaaaga ttttgaatat gccggtgaag
     8881 cattgagcga cgtaatttat tggaaagtag atgaaattct tcaagtagga acatatcgtg
     8941 ccgatttttt tgtggatggt ttcagaattg gaagtttcac ttttgagatc aaaaagtaag
     9001 cggttctaaa acttttgtca tgaaaccagc cagaaaattc catttcgatg cctctaaaat
     9061 tgcttttgat gaagttcatc gaaaaaaaat cgacttcaat atgtcgcgtt atgacttggc
     9121 tgtggaaaca ggcatgaaac gctacaaaga tgtagaagcc gccaaaaacc aggctgctgc
     9181 gataaaacat gaagttcttt cgcatctcga cgaatacctg ttgcagtttg aaaaaaacat
     9241 ttcttctcat ggcgccgaag ttctttgggc acgcaacagc gaagaagcaa tcgtttatgt
     9301 ggaaaaaatt ctggttgaaa acgatgcccg cctgctggtg aaaagcaaat cgatgactac
     9361 ggaagaaatc gaactgaatg ctgctgcagc aaaattgggc tgtgaatctg tggaaacgga
     9421 tttgggtgaa tttattgttc aaacggcagg agaaaagcct tatcatatcg ttacacctgc
     9481 catgcacaaa tcaaaaagtg aaattgccga tttgtttcac gagaaattcg ataccccatc
     9541 cgacagtact ccagaacaat tatcggattt cgttcgtcgt ctgctccgaa aaaaattcac
     9601 gcaagccgag ataggagtta ccggcgccaa ttttctcatt gccgatatgg gtggaatttc
     9661 ggttaccgaa aatgaaggta atgccttaat gagtacttct ttcccgaaaa ttcacattgt
     9721 ttttgccggt atcgaaaaaa taattccgaa gatggaacaa ttgggacttt tttatccgtt
     9781 gcttgccgtt catggtacgg gtcaacaaat gacggcctac aatacgattt ttacaggccc
     9841 acgtcaaacg ggagaaacag atggccctga aaaaatgtat gtaattttgc tcgataatgg
     9901 gagaattggt atttttgcag acgatgaggc ttatcaatca ttggcatgta ttcgctgcgg
     9961 cgcttgtctg aatggctgtc cggtgtacag aaccattggc ggttacacgt acaacaccac
    10021 ttacagtggg ccaattggct cggccatttc tccgttttta aagggatttc gcgattttta
    10081 ccacctgagc tatgcttcaa cactttgtgg aaaatgtgca gaagtttgcc cggtaaaaat
    10141 tccgcttccc gacattttgt tggcaaatag gagaaaagcg gtagaccaac atctctcatc
    10201 ggcgctggag accgcagcaa tgaaaggatt tcgtctgatt gcctcgaaac gaaaaggatt
    10261 tgactttttc cctgcgggaa tgaaaaattc agctttttat cccttaaatt tttggggatg
    10321 gggacccgaa agaactgttc cgaaatttgc aaaaaaatcg ttttcacagc tctacagaaa
    10381 aaacaacaaa taatttaatt tattcattat caaatatatg caaggaggaa aaatataaaa
    10441 cattttgtta aaaaaaaatg ttgaatccaa tttttcttac caaaaaacgt aaaaataatc
    10501 gacttatgaa accaaagagt ttctttcttt ttatttcgac cctactgatt ttttctttcg
    10561 caggatgtaa agaaaataat tatgaagatt ggaaaatcgt gaacgaccag tggatgttga
    10621 aacatcaatc cgattcggga tttgtggtta catccagtgg actttgctat caaatcattc
    10681 atcagagcgt gggaagaaag ccaaatgcaa gtagttggat cacggcaaac tacaaaggat
    10741 atttaattga tggaacattg atcgaaaaag gaacgtacga ctactatctt tcgagttcca
    10801 tcagtggttg gcaagaagga attaccaaaa tgagggatgg ttcaactttc attttttaca
    10861 ttcccagctc acttggttat ggaaaagagg ggtcgggttc aaaaattccc ccatattctg
    10921 cattgattta tgaggtaacc ctcgtttctt cctattattg aaacacaaat ttattcaaga
    10981 aatcaggaaa tgaaaaaaat tttcattctt ttgctgacaa gctttgcaat atgttccatt
    11041 tggagtcaaa cctacaccgt tgaaacgatt cccaatccaa aaaaactgaa tcgttttgcc
    11101 tttgtcagta atcctgatgg tattttgaac cccgaaaccg aaagtcaaat caacaatttg
    11161 ctcgattcat tagaaaaaaa gacaggtgcc gaagttgcaa tcgttttagt caattctatt
    11221 ggcaatgaag atattaaatc atttgccgag caactcttca attcttggaa aataggaaaa
    11281 gaaaaggaag acaacggttt actcgtactt ttcgtaatgg atcaacggaa agttactttt
    11341 gaagtcggtt atggactgga agggattctt cctgatgcta tctgtaaacg tattcaaatt
    11401 gaaagcatga ttcccgaatt taaaaaggga aattatgatg caggcatact ggccggcgtt
    11461 caacgtatct atgccactat tttgaaagaa cccgttcaag ttgaaaaaac agctcctgta
    11521 aattggaaag aaattttgcc gcttgcattg ggtttttatc ttatatggtt ggtattaact
    11581 tggttgtgga tgggtaacgc tatgaataaa gtgaagataa atccgcgact caacagtaat
    11641 atggcacgct acattgctgc aaaaaatgaa agaaacggca ttgtcagcgt tatggcaata
    11701 tttttaccta ttgccgcatt tatcgctatt cttgtcttat cgcaagctat ttatctgctt
    11761 ttgctccttc ctcttccttt catgacaatt cctccactga tatatgccaa ttacaggctt
    11821 caaaaaatca gaaaagaacc ttttccctgc agtgtttgtg ggggacaaat gcactttgtt
    11881 ccggaaaaag aagaagatgt ttacctgaaa ctttcccaac aatttgaaga acaacttcat
    11941 gctgtcgatt acgatgtatt tgtttgcgac aattgtaaaa atgaagccat ttttactatc
    12001 gataggccca gcgcttactc ggaatgtcca aaatgtggaa ccaaagcttt tattctgaaa
    12061 gacaaaaaac ttactatggc ccctacctat ttcaatgccg gaacagaaag gcttacctat
    12121 cgttgtgaat tttgcggcta cgaagaaaac aaaaaccggc gtattccgcg acttacccga
    12181 gccaacagta cctttatcgg aggagcagca gccggcagta ttttcagtgg aagcggaggt
    12241 tttggtggtg gaagttttgg tggcggcatg tcggggggcg gaggcgcaac cagcggttgg
    12301 taaaataaga catttttaaa ttcttcaatt tcaaaaacta taacaatttc aattttaatc
    12361 aatcttacta aaacactttt tttcaatgaa ttacacattt tctaaataaa aaaattatga
    12421 aacgagcatg tatggcagta ttcacaa
//
LOCUS       DAOSCH010000060        12253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3494_length_12253_cov_5.655025, whole genome shotgun sequence.
ACCESSION   DAOSCH010000060 DAOSCH010000000
VERSION     DAOSCH010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12253
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3494_length_12253_cov_5.655025"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="PLZ54_05795"
     CDS             <1..1197
                     /locus_tag="PLZ54_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOJ66473.1"
                     /translation="YGLSIIVIYVTLGLLITLIFGASALNSMSTNAIFNLFFFVLLVV
                     FALSFFGAFELTLPSSWSTKVDAKSESTVGLLSILLMAFTLVLVSFSCTGPIIGTLLV
                     EVASTGSLLAPAVGMFGFAFALAVPFTLFAFFPSWLESLPRSGGWLNRVKVVLAFIEL
                     AFALKFLSVADLAYGWHILDREVFVSLWIIIFALLGIYLLGKIRFPHDSESKHTSVGG
                     LMLGIISLAFAVYMVPGLWGAPLKSISAFAPPLYTQDFNLYNNEVHPAFTDFEEGMAY
                     AAMKKLPVIIDFSGYGCVNCRKMEASVWTDPQVKALLDKNFVLISLFVDDKTPLEKPF
                     EINENGKTRKIKTVGDKWSYLQRYKFGANAQPFYVMIDYHGKPLNKPYVFDTNPSHFV
                     NWLNRK"
     gene            1464..2045
                     /locus_tag="PLZ54_05800"
     CDS             1464..2045
                     /locus_tag="PLZ54_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOJ66474.1"
                     /translation="MEKENKASEKNQSAENEKKHQQQHKENEDNIIPQSENNDAENVE
                     KELERLREECEDLADKNLRLLAEFDNYRKRMLKEKADLLKNGGENVLVNILPLVDDFE
                     RALKAIETSDDIEAVKEGIHLIYGKFVDFLNKNNVKEIPTENVEFNTQYHEAVSKIPA
                     PSEELKGKVIECLSKGYTLNDKVIRYAKVVVGE"
     gene            2119..3270
                     /gene="dnaJ"
                     /locus_tag="PLZ54_05805"
     CDS             2119..3270
                     /gene="dnaJ"
                     /locus_tag="PLZ54_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOJ66475.1"
                     /translation="MAKRDYYEILGVSRTATEVEIKKAYRQKAIQFHPDKNPGDKKAE
                     ENFKEAAEAYEVLSDPEKRERYDRYGHAGVGSSASSGSYGGGMTMEDIFSHFGDIFGG
                     HFGGFSGFGGGSRSSAPRMRRGTDLRVKVKLTLAEIAEGVEKKIKVKKYVPCSHCQGT
                     GAAEGSAPVTCSMCRGTGRVTRVQQTILGQMQTTSDCPTCHGEGKVIKEKCIHCNGEG
                     IIHDEEIVTINIPAGVAEGMQLTLSGKGNAARRGGINGDLIVVIEEEPHSELIRDGND
                     LIYNLLLSVPQAALGGAVEVPTVNGKVKVNIDAGTQPGKVLRLRGKGLPDVNRYGNGD
                     LLINIGVYIPEKLSKEEKAIMEKLEQSSNFKPSSSAQKSFFSRFKDMFD"
     gene            complement(3498..6362)
                     /locus_tag="PLZ54_05810"
     CDS             complement(3498..6362)
                     /locus_tag="PLZ54_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOJ66476.1"
                     /translation="MKSKLLVGIVFVFSFVFPQFTKAVPAYPFPVKVVQPDGTEISVK
                     LNGDEFFHYQTTLDGYLITRDKDGFFKYGKLSKKKEIAPSKYRVNNIEKRTKEEINFI
                     KQLPKGIDFSKQNQLNRIQRASSVTVTEKSGRKAYPLTGSPKSLVILVNFSDNSFTVS
                     SPKEAFTKLLNQNGYSENGSTGSARDYFYDTSTEKFNPQFDVVGPYTLPQTMGYYGEN
                     GSDGMDKNPQQMIIDACRLADNDGVDFSQYDTDRDGYVDNIFVYYAGYNEAEGAPANT
                     IWPHRWTLANYNTKFDGVVVFDYACSSELRSSRGANMCGIGTFCHEFGHVLGLADYYP
                     TDGGTHFTLSYWNIMDAGAYLNDGKTPPTYSAFDRFFLNWLTPIELKSSGNFTLQPLT
                     SSNSAYLISKDGNHNLVGNSPNPSEFFMLENRQKSGWDAYLPGHGLLVTHINFNQSDW
                     ENNTPNNIAGKLGYKIVEADGIGSEQTLAGDPFPGINNITTCSLVLRNGTKLKKSLTN
                     ITENNYINLDFMGEENSPIVGAMSNFLAFETVQGTPSNAQLLKISGKKLKENILLAFS
                     ENKHFEMQLENDFNAEWTKNLILAPIDSVVDTTRILVRYNPTEPSYLDVHNEIISITS
                     KDATPIYLNLQGKSTRPVYVIPPVATEATDVTISSYVAHWEPVTDATGYYFTAYYISN
                     ESSETTEGFDNGLTPSSGWTITAQALSTSASYSGKEVPAIQFKNSGEMIQTENYVLPA
                     IKLSFFVKSFSGNGALKVEAKNDSDKWITIDNVSVTTSLSAIKTYTFTESDHYNQFRL
                     TYQKESGYLIIDDITVGFAKKINYNEKDKWITSTTDTVINLPSNSDCYYKVKASDKTL
                     RNSVILYENITDFSNLIYVHTLPDVGKQLKAYVNREDKTVTITVPNTSTVVRIYDVTG
                     KLIVSEIPRYNKFTIDALKNRIPGQLYIIVAGDRTAKIIM"
     gene            complement(6676..7287)
                     /locus_tag="PLZ54_05815"
     CDS             complement(6676..7287)
                     /locus_tag="PLZ54_05815"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOJ66477.1"
                     /translation="MRNLWQQLRTEGIEDERVLKAMANVPRELFVGNSCVEEAYENTP
                     LPIGHGQTISQPFIVAYMTECLHVEPDDFVLEIGTGSGYQAAVLAQLAKKVYSVEIIK
                     ELGISAKERLKKLHYDNVEIIIGDGYYGWLEHAPYDKIIVTAAANEIPPPLVEQLKNQ
                     GRMVIPVGFPYTTQHLMLITKSGGKIASTTLLPVSFVPFTGKH"
     gene            complement(7913..9109)
                     /locus_tag="PLZ54_05820"
                     /pseudo
     CDS             complement(7913..9109)
                     /locus_tag="PLZ54_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005924840.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9522..12035
                     /locus_tag="PLZ54_05825"
     CDS             9522..12035
                     /locus_tag="PLZ54_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOJ66478.1"
                     /translation="MTVNYSEQLHNILLYSLQEAERLGNNYIGPEHLLLGIIRNGKGK
                     AIDILNHTQVDLSKIKQSIESQIRTDNEPTGEEILSLKSTERIKKIMELETRSLSSET
                     ADSEHLLLAILKEKNNLAVEVLNEENLTYEIAKSYLEHSKDIQMSSNFQDDDDEDDEP
                     RQHQRNVSGSGKQSDTPALDTFGTDITKAALENRLDPVVGRQVEIERLAQILSRRKKN
                     NPVLIGDPGVGKSAIVEGLALRIVQHKVSRVLFDKRIVALDMASIVAGTKYRGQFEER
                     IKAILNELAKNPNIILFIDEIHTIVGAGGAPGSLDAANMLKPALARGEIQCIGATTLD
                     EYRQSIEKDGALERRFQKIIVEPTSYEETLQILRNIQDKYEYHHNVRYTPAAIEACVR
                     LTERYITDRCFPDKAIDALDEAGSRVHIGSVSVPKEIEELEKRIEELRQQKMKVLSEQ
                     KYELAGPIRDQEKQCQYQLEDAIRRWEQESQQHPEIVDEEQVAEVVAMMSGIPVQRIA
                     QAEGLKLRQMKDVLQSKIIGQEEAVNKIVKAIQRNRIGLKDPNKPIGSFIFIGPTGVG
                     KTYLAKVLAEFMFDSPDALIRVDMSEYMEKFSVSRLIGAPPGYVGYEEGGQLTEKVRR
                     KPYSIVLLDEIEKAHPDVFNLLLQVMDEGRLTDSLGRRIDFKNTILIMTSNVGTRQLK
                     DFGKGVGFNTIETPEMDAEFSQGVIQKALSKTFAPEFLNRIDEVIVFNPLSKDAIKSI
                     IDLELKGLFGRITSMGYRLELTPEAKDFIAEKGYDAQFGARPLKRAIQRYLEDELADI
                     VLSGDLETGDTILMDLDAENGKMKAKIVKPAPVLNQNNN"
BASE COUNT         3923 a         2254 c         2448 g         3628 t
ORIGIN      
        1 tatggtttgt ccattatagt catttatgtg acattgggat tgttgataac gcttattttc
       61 ggcgccagtg cattgaacag catgtccacc aatgcaattt ttaatttgtt tttctttgtt
      121 ttgcttgtcg tttttgcctt gtcgtttttt ggtgctttcg agctgacttt gccttcatcg
      181 tggtcaacaa aagtggatgc caagtccgaa tcaacggtag gtttgctgag tattttgtta
      241 atggctttta ctttggtgtt ggtttccttt tcctgcaccg gtcctattat agggactttg
      301 ctggttgaag ttgcatccac cggttctttg cttgctccgg ctgtagggat gtttggattt
      361 gcttttgcgt tggctgttcc tttcactttg tttgcattct tcccttcgtg gctcgaatcg
      421 ttgccacgct caggcggttg gctaaacagg gtaaaagtag tgctggcttt catcgaactt
      481 gcttttgcac tgaaatttct ttcggttgcc gatttggctt atggctggca tattttggac
      541 agagaagtat ttgtttcgct gtggattata atttttgctt tgctggggat ttatttgctc
      601 gggaaaattc gttttccaca cgatagcgag tcgaaacata cttctgttgg tggacttatg
      661 ctgggaatca tttctttggc ttttgccgtt tacatggttc ccggactttg gggagcaccg
      721 ctgaaatcga tcagcgcttt cgctccgcca ctttatacgc aggattttaa tttgtacaat
      781 aatgaagttc atccagcttt taccgatttt gaggaaggaa tggcttatgc tgcaatgaaa
      841 aaactgccgg ttattattga cttttcgggt tatggttgtg taaattgccg gaaaatggaa
      901 gcttcggttt ggactgatcc acaggtgaaa gcattgctcg acaaaaattt tgtgctgatt
      961 tcgctgttcg tcgatgataa aacgccattg gaaaaacctt ttgaaattaa cgaaaatggt
     1021 aaaacaagaa aaattaaaac cgttggtgat aagtggagct atctgcaacg atataaattt
     1081 ggagccaatg cacaaccttt ttatgttatg atagactatc acggaaaacc tctgaataaa
     1141 ccctatgttt ttgacaccaa tccttctcat tttgtcaatt ggttgaatcg caaatagtac
     1201 attttgtctt aatcagccct atgtgttttt atttttctgt tattttgtca tttaattcat
     1261 tgtttggtag aaacattgta ttcaaaatag ttgattggca aacttattgt ttaatatgag
     1321 aatgagaaat tttttgagaa acttatcaga gaattgtctt gtaattctga tttagaaaaa
     1381 ataagttgga atgaattttt tgtgttatat agaaattgat tagctgaact taagagttta
     1441 ccaattaaaa aagggaggaa aaaatggaaa aggaaaataa agcgtcagaa aaaaatcaat
     1501 ctgccgaaaa cgaaaaaaaa catcagcaac aacataagga aaatgaagac aacataattc
     1561 cacaatcaga aaataatgat gctgaaaatg tggaaaaaga gttggagcga ttgagggaag
     1621 aatgcgaaga tttggcagac aagaatttgc gtttgttggc cgagttcgac aattatcgca
     1681 aacgtatgct caaggaaaaa gccgatttgc tgaaaaatgg cggagaaaat gtgttggtga
     1741 atattttgcc gcttgtcgat gactttgagc gggctttgaa agctattgaa accagcgatg
     1801 acattgaagc tgtgaaagaa ggaattcatt taatttacgg taagtttgtc gattttttga
     1861 acaaaaataa tgtgaaggaa attcctactg aaaatgtaga atttaacact cagtatcacg
     1921 aagcagtatc caaaattccg gcaccttctg aagaattaaa agggaaagta attgagtgtt
     1981 tatcgaaagg ctacacgttg aacgacaagg taattcgata tgcaaaagtc gtagtaggcg
     2041 aataatttta ttcgcctttt aattttttga attttttttg tttacaaata tgttgaggat
     2101 gagggagaat taagttttat ggcaaaaaga gattattacg aaatcttagg tgtttcacga
     2161 acagccacag aagtagaaat taagaaagca taccgtcaga aagccattca gtttcatccg
     2221 gataagaatc cgggcgacaa gaaggctgaa gaaaacttca aggaagctgc tgaagcttac
     2281 gaagttttaa gtgatcctga aaaacgtgaa cgctacgatc gctatggaca cgcgggagtt
     2341 ggaagttcag cttcgagtgg aagttatggt ggcggcatga ctatggaaga tattttttcc
     2401 cactttggcg atatttttgg cggtcatttt ggcggctttt cgggttttgg aggtggatcg
     2461 agaagcagcg ctccccgtat gcgaagagga accgatttgc gggtaaaagt caaattaaca
     2521 ttggctgaaa tagctgaagg agtcgagaaa aaaataaaag tcaagaaata tgttccctgt
     2581 tctcactgtc aaggaaccgg agcagccgaa ggttcggcgc ccgtaacctg ttcgatgtgt
     2641 cgtggaaccg gaagggttac gcgcgttcag caaactattc tcggacaaat gcagactact
     2701 tccgattgtc caacctgtca tggggaagga aaagtgatta aggaaaaatg tattcactgt
     2761 aacggagaag gaattattca cgacgaagaa atagttacca tcaacattcc tgcgggtgtg
     2821 gctgaaggaa tgcagctaac tttgagcggg aagggaaatg cggctcgcag aggtggaatc
     2881 aatggcgatt tgatagtggt gatagaggaa gagcctcatt cggaactgat tcgcgatgga
     2941 aatgatttga tttacaattt gttgctgagt gttccgcaag cagcattggg tggagctgtt
     3001 gaagttccaa ccgttaacgg caaagtgaaa gttaacatcg atgccggcac acaaccgggt
     3061 aaagtactga gattaagggg caaaggatta cccgatgtca acagatacgg caacggcgat
     3121 ttgctgataa atattggcgt atatatcccc gaaaaattga gtaaggagga aaaagccata
     3181 atggaaaagc tggaacagtc gagtaatttt aaacccagtt cctcagctca aaagagtttt
     3241 ttcagtcgtt ttaaagatat gttcgattga attcgaaatt tgtatttcat ttcgaaaaac
     3301 aggggaaaag gagagaaaaa gtgatttctt tccttttttg gtaagaaaat aatttgccga
     3361 ttccgccaaa gagtattaac cgaatcttaa atttctgaaa tatcaacaag tatatccaat
     3421 taaaaacaaa ttagattgta aaatttttat tttataaatg caattatcta cttcaacaat
     3481 aagctttaat aaacttttta catgataatt ttcgctgttc tgtcacctgc cacaataata
     3541 tagagttgtc ccggtattct gttttttaat gcatcaatcg taaatttgtt gtaacgagga
     3601 atttccgata caatcaattt ccctgtaaca tcataaatcc ttactaccgt actggtattc
     3661 ggaaccgtaa tggttaccgt tttatcttct ctgttgacgt aagctttcaa ttgtttgcca
     3721 acatcaggca aagtatgcac ataaatcaaa tttgaaaaat cagtgatatt ttcataaaga
     3781 attactgaat ttctcaatgt tttgtcgctt gccttcactt tatagtagca atcggaattg
     3841 gatggtaaat taataaccgt atccgttgtt gaggtaatcc atttatcctt ttcgttataa
     3901 tttatttttt ttgcaaaccc caccgtaata tcgtcgatga tcaaatatcc gctttccttt
     3961 tgataagtta atcgaaattg attataatgg tcagattcgg taaaggtata agttttaata
     4021 gcactgagac tggtagttac tgaaacattg tcaatggtta tccatttgtc tgagtcattt
     4081 tttgcttcaa ccttaagtgc tccattccca gaaaaagatt tgacgaagaa agataatttt
     4141 atggccggca atacataatt ttccgtctga atcatttcac ctgagttttt aaattgaata
     4201 gccggaactt cttttccact gtatgaagcg gacgtactca aagcttgagc ggtaattgtc
     4261 catcccgatg atggagtcaa accattatca aaaccttcag tcgtttctga tgattcattg
     4321 gaaatataat aggcagtgaa ataatatccc gtggcatctg ttactggttc ccaatgagct
     4381 acgtaactgc tgatcgttac atcggtagct tcagtggcaa caggaggaat aacataaaca
     4441 ggtcttgtgg attttccctg taaattcaaa tagattggag tggcatcttt agaagttata
     4501 gaaataattt cattgtgaac atcaagataa gacggttcgg ttgggttata acgtaccaaa
     4561 attcgggtag tatccactac ggaatcaata ggagcaagga ttaagttttt agtccattct
     4621 gcattaaagt cattttccaa ttgcatttcg aaatgtttat tttctgaaaa agcaaggaga
     4681 atattttctt ttagtttttt tccggaaatt tttaaaagtt gtgcatttga tggcgttccc
     4741 tgaacggttt caaaagctaa aaaattactc atagccccga caatcggaga attttcttcc
     4801 cccataaaat ctaaatttat ataattgttt tcagtaatat ttgtgagaga tttttttagt
     4861 ttcgtaccat ttcttaaaac caacgaacag gttgtaatgt tatttattcc tggaaacgga
     4921 tctccggcga gtgtttgttc agaaccaata ccgtcggctt caacaatttt ataacccagc
     4981 tttccggcaa tattattggg tgtattgttt tcccaatcgc tttgattgaa attgatatgg
     5041 gtaacaagca agccgtgtcc gggtaaataa gcgtcccacc ctgacttttg cctgttttcc
     5101 aacataaaaa actctgaagg attgggtgaa tttccaacca aattatgatt accatctttt
     5161 gaaataagat aggcgctgtt ggatgaggtt agaggttgca atgtgaaatt tcccgaactt
     5221 ttcagttcga tgggtgttaa ccaattcagg aaaaatcgat cgaaagccga ataagtggga
     5281 ggcgttttcc catcattcaa ataagctccg gcatccatga tattccagta agataaggta
     5341 aaatgtgttc ctccatcggt aggataataa tcagccagac caagcacatg accaaattca
     5401 tgacagaaag taccgatgcc gcacatgttt gcgcctctgc ttgaacgcaa ttcagaagaa
     5461 caggcataat cgaaaacgac aacaccatcg aattttgtat tgtaattggc caaagtccag
     5521 cgatgtggcc atatggtatt ggccggtgct ccttctgctt cattgtagcc ggcataatag
     5581 acaaaaatat tatcgacata tccatccctg tcggtatcgt attggctaaa atctacacca
     5641 tcattgtcgg ccaatctaca tgcgtcaata atcatttgtt ggggattttt atccatgcca
     5701 tctgagccat tttcaccgta atagcccatt gtttgtggca aagtataggg ccctaccaca
     5761 tcaaattggg ggttaaactt ttcggttgaa gtgtcgtaaa aataatcacg cgccgagccg
     5821 gttgaaccat tttcggaata tccattttga ttcaaaagtt tggtaaaagc ttcttttggt
     5881 gaactgacag taaatgaatt gtctgaaaaa tttactaaaa ttaccaatga tttcggactt
     5941 ccggtcaaag gataagcttt acgaccggat ttttcggtaa cagtaacaga tgaagctcgt
     6001 tgtatgcggt tcaattgatt ttgtttcgaa aaatctatcc ctttgggaag ttgttttata
     6061 aaattaattt cttccttcgt acgtttttcg atattgttta cgcgatattt agacggagct
     6121 atttcctttt ttttacttaa ttttccgtat ttgaaaaatc cgtccttgtc cctggtaatc
     6181 aaataaccat ctaaagtagt ctgataatga aaaaattcgt ctccattcag tttaacagaa
     6241 atttctgtac cgtcaggctg aaccaccttt accgggaaag gataggcagg gactgctttt
     6301 gtaaattgtg gaaaaacaaa actaaaaaca aatacgatac cgacaagcaa tttacttttc
     6361 ataaaataaa gcaaaaatta taaatcacag aatttgttta aaaataacaa aataataatt
     6421 tggtgaatta atacagattt tagaaaaata tttcctaaat tcaaacaatt caaacaattt
     6481 ttttcataaa aaacatgaac cttagaacaa agatacaaaa tccggtcaat gatgcaacta
     6541 agtattgaaa aaaatatggc atcagttaat gatagaggcc aattggttgc ttaaattgaa
     6601 cggcagcgaa attacgaaaa tagaaacaag caatggactg tagatttggc gcaacgtaaa
     6661 acattactct tctcttcaat gttttcccgt aaagggaaca aaactcacag gcaacagagt
     6721 ggtggaagca atttttccgc cactttttgt aatcagcatc aaatgttgtg tagtataggg
     6781 aaaaccaacc ggaattacca ttcgtccctg atttttaagt tgttctacaa gtggtggcgg
     6841 aatttcgttt gcagcggcag taactataat tttatcgtaa ggagcatgtt ccagccaacc
     6901 gtaatagcca tctccaatta ttatttctac attgtcgtaa tgtaattttt ttagtcgttc
     6961 tttggcagaa attcccaatt cttttataat ttctaccgaa taaacttttt ttgccaattg
     7021 agcaagtact gccgcctgat aaccggaacc cgttccgatt tcaagaacaa aatcatccgg
     7081 ttcaacatgc aaacattcgg tcatgtaagc tacaataaaa ggctgagaaa tagtttgccc
     7141 gtgtccaatg ggtaacggcg tattttcata agcttcttcc acacaagaat tacctacaaa
     7201 caattcgcgt ggtacgttcg ccattgcttt cagcacacgt tcgtcttcaa ttccttccgt
     7261 tctgagttgt tgccaaagat ttctcatgtc aaaataaaaa ctacaaaaat aatttgttta
     7321 ttatcaatta taaaaacaaa ttcttgtaaa tttttgtttg ctcatgaaag aaaaaagcag
     7381 cctgaaaatt ttcaaaccgc cttttcttta aataaaaata ccgaaaaatt tattcctttt
     7441 gaatattggg tagctccaaa ccatagtttt tcgctttatc ttcctttttc aacaagaaag
     7501 caaacagcaa ggccagaact ccaaaaatca taaataccag acttggacgt gtatagtcgt
     7561 acctcaaagc aattttattt tttacgattt ccgaataacc aatctctaag caagtatttt
     7621 ctaccgctcg aagtttctga tttttgtctt gaaaagaatt tagtttttcc aatggcaaag
     7681 cggtaatatt tttatgtatc cattcatttt taaaggctgc aaaacctacg gtatcctgca
     7741 caccttcata atcgcttttc aacaacactt cattgattaa agccgtagta gttttatcaa
     7801 cggctttctc aaattcaatt ttattcagtc ccaatgaagg ttgatataat tcataagttt
     7861 gcaccacacc cgtcttcacc atttctttcg ctttgtaaac cgtaggattt gcactgttca
     7921 gcaccaatcc gatcaacaat ggaacaccca ttaatcccca attttgtatc cagaaaatca
     7981 atgcgtaagc tgttcccaac tgcctttccg gaataatttt cggaactgat ggccacatgg
     8041 ccgaaggtac aagcgataag gcaattccca aaactatcgt tacgccaata gccaagaccc
     8101 aattattgag aaatggaata gaaaaaattg catgaacaaa tataagcaat aaagcgccaa
     8161 gaatcataat ggaagcacct ttgccctttc ggtcgtaaat atttccaaat aatggagtaa
     8221 gaaacaatgt tcccaaagga agaacgctcg ggataagccc tgccaaatcc ggattaacct
     8281 gatacttgtt gataaccata tcggtggcaa attttatcca cggaaaaact gcggaataaa
     8341 acagaacaca caaaatggca ataagccaaa aacctttatt tttaataata gtgaaaactt
     8401 ccgaaacttt aaacggttct tcctcttcct tttcatcatt tgcttctgaa gcttccagct
     8461 ttttgtccat ctgaacgtaa acaatataag cgataagtcc aatacagagc agtaaaacgc
     8521 caaacaggat aggaaccgaa acatcattaa aatgtttggc aacagggagc ggaatagcga
     8581 cggccagcaa agttccaagt cgagctgtag ccatttgaag tcccatagcc agcgccatct
     8641 cttttccttt aaaccatttg acaatgacac gagaaaccgt aatgccggca atttctactc
     8701 cgacggcaaa tgtggcatat cccaatgccg ctatcattac ctgagccttg atgcccagaa
     8761 tagtcccggc atcatcgggg aaaaaaggcg atatggcaaa atacttgatg gctgccccaa
     8821 tcaccatgac ggctgtagag gccaatcctg taaaacgaac acccattttg tccaaaatga
     8881 taccaccaaa aataagcatc aacagaaata cattgaacca tccataagca ctggtaaaca
     8941 gcgaataatc aatgctactc catagcaact gactctccag caatggttta agaggactca
     9001 tggcatcggc aatgaaatat ccgcaaagca tggtaaagga aactattccc aaagccgtcc
     9061 aacgtgccac cggtgattca cgtaatgaaa ctgattttat ctgcgacata aacaattttc
     9121 tttaatggtt gtttttttat tgaatttatg ttcttggtat ttaaaaataa agaaatgcaa
     9181 aactactgaa aaaatttttg aaaacaattt catgcaggca ttcctattca tatttttttg
     9241 cagcctgaca acagttcact ttatactgac aaaatttcag aagacacgat aaaaacaaat
     9301 cggtattatt tttgatagat aaaataaaat tttggagtga aaagtttgat aataataagc
     9361 acagtaaacg atttcaagat taaagtttta ctaatgttga catgaaaaaa ttttaatcca
     9421 ttcgttttct tataaaaaat taaaatttct aaagtcaaac tttgagtgtc caacatcaca
     9481 ttaaattaat tatttttgta gaaaaaagga gaaatctaaa tatgaccgtc aactattcag
     9541 aacagttaca caacatactt ctttacagcc ttcaggaagc tgaaaggttg ggaaacaatt
     9601 atatagggcc tgaacatttg ctgctgggga taatcagaaa cggcaaaggg aaagctatcg
     9661 acattctcaa ccacacgcaa gtggacttgt caaaaataaa acagtcaatc gaaagtcaga
     9721 ttcgtacaga taacgaacct accggagaag aaattttaag tttgaaaagc accgaacgca
     9781 ttaaaaaaat catggagctt gaaactcgtt ctctttcatc cgaaacagcc gattctgaac
     9841 atttgctttt ggccattctg aaagaaaaga acaatttggc tgtagaagtt ttaaatgaag
     9901 aaaatctgac ctatgaaatt gccaaatcgt atcttgaaca ttctaaggat attcaaatga
     9961 gttccaattt tcaggatgat gatgacgaag atgatgaacc tcgccaacat cagcgaaatg
    10021 tttcgggaag tggcaaacag agtgatactc cggctttgga cacattcgga acagatatta
    10081 ccaaagctgc tctcgaaaac aggttggatc ccgtagtagg cagacaagtc gaaattgaaa
    10141 gattggctca gattcttagc cgtagaaaga aaaacaatcc cgtattgata ggtgatcccg
    10201 gagttggtaa atcggctata gtagaaggtt tggctttgcg catcgttcaa cataaagttt
    10261 cgcgtgtact cttcgacaaa cgaattgttg cattggatat ggcttccata gtggcaggga
    10321 caaaatatcg cggacagttt gaagagcgca ttaaagctat tctcaacgaa ttggcaaaaa
    10381 atccgaacat cattcttttt attgatgaaa ttcacaccat tgttggtgcc ggtggcgcac
    10441 ccggttcact ggatgctgcc aacatgctta aacccgcatt ggcacgtggt gaaattcaat
    10501 gtatcggtgc caccactttg gacgaatacc gccaaagcat cgaaaaagat ggagcgttgg
    10561 aacgtcgatt ccagaaaatt attgtagaac cgacttctta cgaagaaaca ttgcagattt
    10621 tacgcaacat tcaggataaa tacgaatacc atcacaacgt gcgttatacg ccggcagcca
    10681 ttgaagcttg cgtaagactt accgaacgtt acataacaga tcgatgcttc cccgacaaag
    10741 ctattgatgc attggacgaa gccggttcgc gcgttcatat aggctcagtt tccgtaccga
    10801 aagaaattga agaattagaa aaacgtatag aagaacttcg tcagcaaaaa atgaaagttc
    10861 tttcggaaca gaaatatgaa ttggccggtc caatacgcga tcaggaaaaa caatgccaat
    10921 accagctgga agatgcgatc agacgatggg aacaggaatc acaacagcat cccgaaattg
    10981 tagacgaaga acaggttgcc gaagtagttg ccatgatgtc gggcattccc gtgcagcgta
    11041 ttgctcaagc agaaggactg aaactccgac aaatgaagga tgtacttcag agcaaaatta
    11101 tcgggcagga agaagctgtg aacaaaattg taaaagccat tcaaagaaac cgtattggat
    11161 tgaaggatcc aaataaaccg attggttctt tcatatttat cggccccaca ggcgtaggaa
    11221 aaacttatct ggccaaagtg ctcgccgaat ttatgttcga cagtcccgat gcactgattc
    11281 gcgtcgatat gagcgaatac atggaaaagt tcagcgtatc gcgacttatc ggagcacctc
    11341 cgggatatgt gggttacgaa gaaggcggtc agctgaccga gaaagttcga cgcaaacctt
    11401 attcaatagt cttgctggat gaaattgaaa aagcgcatcc cgacgttttc aaccttctcc
    11461 ttcaggtaat ggatgaagga agattgactg acagcttggg tcgcagaata gattttaaaa
    11521 ataccattct gattatgact tccaatgtcg gtacgcgaca actgaaggat tttggtaaag
    11581 gcgtaggatt caataccatt gaaacacctg aaatggatgc agaattttca caaggtgtaa
    11641 ttcaaaaagc gctgagcaaa acatttgctc ccgaatttct gaaccgtata gacgaagtaa
    11701 tcgtatttaa tccgttgagc aaggatgcca ttaaatctat tatcgacctt gaattaaaag
    11761 gactttttgg acgaattact tctatgggtt atcgactcga attgacaccc gaagccaaag
    11821 atttcattgc cgaaaaaggt tacgatgctc aatttggtgc acgccctctg aaacgtgcta
    11881 ttcaacgata ccttgaagat gaactggcag acattgtgct gtcgggagat ttggaaacgg
    11941 gcgatacaat tttaatggat ttggatgccg aaaatggcaa aatgaaagca aaaattgtta
    12001 aacctgctcc ggtactgaac caaaacaaca actaaaatat caagcaaaaa ttgaaaaatg
    12061 tacctgctat tcgatgcaat ggcaggtatt tttttgtctg ttttgaatga aaatcgtgcc
    12121 aaatatctct ttaaaaccgc atctattgtt gtaggataaa agaaaaagaa gtatatttgc
    12181 accgcatttt gagaaatgtt ggagagatgg cagagtggtc gattgcggcg gtcttgaaaa
    12241 ccgttgaact gaa
//
LOCUS       DAOSCH010000061        11958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3595_length_11958_cov_6.748635, whole genome shotgun sequence.
ACCESSION   DAOSCH010000061 DAOSCH010000000
VERSION     DAOSCH010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3595_length_11958_cov_6.748635"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PLZ54_05830"
     CDS             <1..275
                     /locus_tag="PLZ54_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66479.1"
                     /translation="LLIVAGLLAADFFLIDFSSSLTTRYLIFGLIIPFVCLGYLIFKA
                     ENAHDFHQTSTLSKVIMLLGVSYSFVFYFLQAQQYHFPIFHIFMVQ"
     gene            363..1181
                     /gene="yaaA"
                     /locus_tag="PLZ54_05835"
     CDS             363..1181
                     /gene="yaaA"
                     /locus_tag="PLZ54_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOJ66480.1"
                     /translation="MAFSQKKKFTSKEFTAMLMLLSPAKIQNTKLPKNSYPATIPQFL
                     DDAEYLVEKVRELSLRELAQLLGVNSQLAELNRDRFYSWHRPFTVDNARQAVLVYNGE
                     VFRGLNAASMTENELTYLQQHLRILSGLYGVLRPLDLIQPYRLEISSRLPTFAGNDLY
                     DFWGKKITDAVSQAFQFSGQPEILLNLCSSEYIKAIDRDFFDGKIVDIEFLENDHGNF
                     RPIVVYLKKARGLMVRYVAQYRIENVEDLKGFDVEGYWYHPELSNDNKMVFVRG"
     gene            complement(1649..2707)
                     /locus_tag="PLZ54_05840"
     CDS             complement(1649..2707)
                     /locus_tag="PLZ54_05840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOJ66481.1"
                     /translation="MSNLLIFGDNLKGLKYLLEEKELKGQIDLVYIDPPFATGGNFVI
                     SNGRATTISNSKNGDVAYSDNVKGSDFIEFLRQRLEILKDLLSERGSIYLHTDYKIGH
                     YVKVMMDEIFGVENFRNDITRIKCNPKNFSRIGYGNIKDMILFYTKTSNPIWNEPKEK
                     HKEVNIEKLYPKKDKNGRRYTTVPIHAPGETSSGDSSKPFKGLLPPPGRHWRTDIKTL
                     EKWDNEGLIEWSSTGNPRKIIYADESEGKRVQDIWEFKDPQYPDYPTEKNSDMLDLII
                     KTSSNENSIVLDCFCGSGTTLKSAHLNNRNWIGIDQSSLAIDAAKRKLGEISASLFVP
                     KPEYEFVDLMENLLAYNS"
     gene            complement(2712..3494)
                     /locus_tag="PLZ54_05845"
     CDS             complement(2712..3494)
                     /locus_tag="PLZ54_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019036314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOJ66482.1"
                     /translation="MNKWINLSIEYANQKSYLDDLFQVYPTIPEGIREIDVQIWREVE
                     NAFIKRDNYRLIKELLKLDLFPIKDSYIAYLKRDSSAIDRNPRTINRICGRIYELGLN
                     KLYERCSEPKETNRQIGPMFKDWLRNKSLGILPVEIDEFMSGNNDAILDGGDKVLMDF
                     AKEKLGYYHDKGLDFVARFNGKYVIGEAKFLTDFGGHQNAQFNDAISTLEVKKVKAIK
                     VAILDGVLYIKGNNKMYKSITQKFNNYNIMSALVLREFLYQL"
     gene            3848..4759
                     /locus_tag="PLZ54_05850"
     CDS             3848..4759
                     /locus_tag="PLZ54_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008626043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOJ66483.1"
                     /translation="MGITQLVDIDLKEIVPFIDWNFFFLAWRLPGKYEGIENLCNCTS
                     CEVGWLQQFPENNRTKAEEALKLFRDAQQMLQDFLHEKSISIHAIFGFFRAVSQGDDL
                     LISFGDQTYILPTLRQQHASSDGFCYSLADFINTENDYMGAFATTVEGAETMAENFER
                     EDDLYRAIMVKTLSDRLAEAAAEWLHYKVRTEFWGYAPDEPLEIKNILKGKYQGIRPA
                     VGYPSLPDQSIIFELQSLLGLEKIGIRLTENGAMFPNASVCGLYFSHPKSRYFMIGKI
                     NDEQLEDYARRRGKTVQEMKKWLAANI"
     gene            4835..5047
                     /locus_tag="PLZ54_05855"
     CDS             4835..5047
                     /locus_tag="PLZ54_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HOJ66484.1"
                     /translation="MLEKSLKIKGMSCSHCATRVEKALNSLDGVEAKVDLASNSAKLT
                     LAKELSNEEIKKAVENVGYEVTEISE"
     gene            5565..6554
                     /locus_tag="PLZ54_05860"
     CDS             5565..6554
                     /locus_tag="PLZ54_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66485.1"
                     /translation="MKLEEAYSWIDPLYQFASLLVYPMYYIYFRLLTVDRKFSFKKHF
                     VYLLPSTILFVLYSIAVFFTPQEEYKAFLFGEEHHSLNHLSYIEIVSFLIRIVFLIQV
                     ILTVIGNFRLIRKYGHKAAQYYSNMEDSSISKVRLLNYTLIITAISSFILGMLGRGFF
                     ASEITYLAIASVVFSSMLFAIGWLGNKQKSLNPAYETDSITETKQKSEELSLIRQEEL
                     LNKIEYFFNDRKIYLNSSLTILDVARLTGTNRTYVSSLINHHFHQNFSAFVNSYRLKE
                     LEEKILFNPEYPHQILAETCGFGSIDSMKRAINLRYKLSLQEFKSEVLLKKVV"
     gene            complement(7321..7551)
                     /locus_tag="PLZ54_05865"
     CDS             complement(7321..7551)
                     /locus_tag="PLZ54_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66486.1"
                     /translation="MNNTDYIYENFQKGNEIYIMDDIEEVAIRYSYSKDGYKTFAKFK
                     GGREYKIDETSNIVTRADMGGTILTKEQYKKF"
     gene            complement(7548..7760)
                     /locus_tag="PLZ54_05870"
     CDS             complement(7548..7760)
                     /locus_tag="PLZ54_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66487.1"
                     /translation="MIFAKEKPEDAKKIIGEKRWNEIHKETGLEGGLGHKFYEEYRST
                     SKISKEGRKRRNELIDLSTKYYNKFR"
     gene            complement(8033..9262)
                     /gene="clpX"
                     /locus_tag="PLZ54_05875"
     CDS             complement(8033..9262)
                     /gene="clpX"
                     /locus_tag="PLZ54_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOJ66488.1"
                     /translation="MPKNVRHCNFCGRSEAEVEFMILGQDGVQICNECAIQAAEIVRE
                     SARKSKSSHLHIEKIPKPKEIKQYLDQYVIGQEEAKKYLSVAVYNHYKRLMQKTEEDE
                     VEIEKSNIIMVGPTGTGKTLLARTIAKKLHVPFTIVDATVFTEAGYVGEDVESILTRL
                     LQVADYDVKAAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQGLLKLLEGSVVNVP
                     PQGGRKHPEQKMIPVNTQNILFICGGAFDGIEKKIAARLNTRVVGYGASEENKLIDRN
                     NLLQYIAPQDLKSFGLIPEIIGRLPILTYLEPLDRDALRRILTEPKNSIIKQYVKLFK
                     MDNIELIFEDEVLDYIVDKAIEFKLGARGLRSITETIMMDSMYEMPSKKSTKWVVTLP
                     YAQSKIEKTNVHRLKIA"
     gene            complement(9273..9971)
                     /gene="clpP"
                     /locus_tag="PLZ54_05880"
     CDS             complement(9273..9971)
                     /gene="clpP"
                     /locus_tag="PLZ54_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOJ66489.1"
                     /translation="MNKKNIRSDFRNYATRHLGMNGLAIDQYAGFINDSYISPTILEE
                     RQLNVTQMDVFSRLMMDRIIFLGTEIDDYASNVIEAQLLYLDSAEPGKDISLYLNTPG
                     GSVYAGLGIYDTMQYISSDVSTICTGMAASMGAILLVAGTKGKRFALKHSRIMIHQPM
                     GGAQGQASDIEITAREIQKMKQELYNIIAEHSGNPYEKIEKDADRDYWMTSQEALEYG
                     MIDKVLINERKSKK"
     gene            complement(10089..11444)
                     /gene="tig"
                     /locus_tag="PLZ54_05885"
     CDS             complement(10089..11444)
                     /gene="tig"
                     /locus_tag="PLZ54_05885"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOJ66490.1"
                     /translation="MNIVKKDLDQNNAVVTLHVEKADYAENVEKNLRDLRKKANIPGF
                     RPGMVPMSLLKKMYGKSVMADEINKLIAEKLYDYIRENDLNILGDPLPNETEQPEIDF
                     NTQEEFDFVFDLGLAPDFDIELSKEIKINYYNIAVTDQMIEDRIKSYSQRFGRYEEVE
                     TVEENDMLRGEVIELENGEEKQNGLQVSEAILTPAYIADPEQKTLFLNAKKGDTVTFN
                     PAKAFGNETELASFLKISKEQAKEITSDFSFKINDITRYFDSEINQELFDKVFGENAV
                     TNEQEFREKVKETIATNLKENSDYRFGIDAKKTLVEKFKNLSFPDAFLKRWLLASNKN
                     LTNEQLESDYPKMIEDLTWQLIKEKLIKKFDIKVNEVEVENYAKEFARAQLAQYGISG
                     ADEELISNYAKSLLEKEDTLRNFIERAGDNKVIVTIKDSVEVEVKEISPEEFDKLFEE
                     N"
BASE COUNT         3487 a         2210 c         2003 g         4258 t
ORIGIN      
        1 ttttgctcat tgttgccggc ttgctcgcag ccgacttttt cctcattgat tttagtagta
       61 gccttactac cagatacctg atttttggtt tgataattcc ttttgtttgt ttggggtatc
      121 tgatttttaa agctgagaat gcccacgact tccatcaaac ttctactctt tcaaaagtta
      181 tcatgttgtt gggtgtttcc tatagctttg tgttttattt cttgcaagcc cagcagtatc
      241 atttccctat ttttcatatt tttatggttc aataaatcgg gaaaaatcat ccatcactgt
      301 cattcattga caattggtaa tcgggcttta tcgttttatg ataaaattga actaacacgt
      361 ttttggcttt ttctcaaaaa aaaaaattca cctcaaaaga atttacagct atgttaatgt
      421 tgctttctcc cgcaaagatt caaaacacca aactcccaaa aaattcatat cctgccacca
      481 taccgcaatt tttggatgac gccgaatatc tggttgaaaa agttcgcgaa ctttcactgc
      541 gggaactggc tcaattgctt ggggtcaact cacaattggc cgaactcaat cgcgatcgtt
      601 tttacagttg gcatcgccct tttactgtcg acaatgcaag gcaggctgtg ttggtttaca
      661 acggggaagt ttttagagga ttgaatgctg cttccatgac agaaaatgag ttaacctatt
      721 tgcagcagca tttaagaatt ttatccggtt tgtacggcgt tttgcgtcct ctcgatctta
      781 ttcagcctta tcgcttggaa attagcagcc gcttgccaac ttttgcaggc aatgatttat
      841 acgatttttg gggaaagaaa atcacagatg ccgtttccca agcttttcaa ttttccggtc
      901 aacctgagat tttacttaat ctctgttcca gtgagtatat taaagccata gacagagatt
      961 ttttcgatgg taaaattgtt gatatcgaat ttttggaaaa tgatcatggg aattttcgtc
     1021 caattgtcgt ctatttgaag aaagcacgcg gcttgatggt gcgctatgtt gcccaatatc
     1081 ggatagaaaa tgtggaggat ttgaaaggtt ttgatgttga agggtattgg tatcatccgg
     1141 aattgtctaa cgacaacaaa atggtttttg tcagaggata aggcaaattc tctcgttgtt
     1201 tttccacaaa tctatgccga ttaaatctca tttaaagcaa aatcaaacaa tttagaaata
     1261 aattaaaaag acatttagat acatttttgc ttcaaaagtg gaagcatttt ttggctttat
     1321 caaacataaa tactactgtc cgataaaaaa atagaaaagg aaagaataaa aaaggggaag
     1381 ttactcaaat tagataactt ccccgacata tagtattttt gtttttgcac aaacataaaa
     1441 actatatggg caaaaatcca gaaagaaatt tggtctttca gccggacttt cggattgaca
     1501 tttaatgaag ttcttttgat tgatcttacg acgataaggc tatttaggga cattctcaag
     1561 gaagttgggc gaaatgccca aaaagcccga cacaaaaaaa atggtttttt ccattgtggc
     1621 aacattagta cggatagatt taatcaattt atgaattgta cgccaacagg ttttccatca
     1681 aatcgacaaa ttcatattca ggcttgggca caaacaaact ggcagaaatt tcacccaatt
     1741 ttctttttgc tgcatctatt gccagagatg actggtcaat gcctatccaa tttctgttgt
     1801 ttaagtgagc tgattttaaa gttgttcctg acccacaaaa acaatcgagt acaatactgt
     1861 tttcgttcga ggaagtttta ataattaaat ccagcatatc tgaatttttt tctgtaggat
     1921 aatcgggata ctgaggatcc ttaaattccc aaatatcctg cacccttttc ccttcacttt
     1981 catctgcata aataattttt ctcggattac ctgtcgaaga ccattcgatt aagccttcat
     2041 tatcccattt ctcaagcgtt ttaatgtctg tacgccaatg ccttccgggc ggaggcaata
     2101 aacctttaaa aggtttactc gaatctccac ttgatgtctc accaggagca tgaatcggaa
     2161 cggttgtgta tcgccttccg tttttgtctt ttttcggata aagtttttcg atattaactt
     2221 ctttatgttt ttcttttggc tcattccata taggatttga tgttttggta tagaataaaa
     2281 tcatatcctt aatattccca taacctatgc gagaaaaatt tttcggatta catttaattc
     2341 ttgtaatatc gtttctgaag ttttctacac caaaaatttc gtccatcatt actttaacgt
     2401 aatgtcctat tttataatca gtatgtaaat aaattgagcc tcgttctgaa agcaaatctt
     2461 ttaaaatttc aagcctttgc cttaaaaact caataaaatc actacctttc acgttatcgg
     2521 aatatgcaac atctccattc ttagaattgc tgatagttgt tgccctcccg ttagaaataa
     2581 caaaattgcc tcctgtagca aatgggggat caatatacac caaatctatt tggcccttta
     2641 attccttttc ttccaataaa tacttaagtc ccttaagatt gtctccgaaa ataagtaggt
     2701 tgctcataga tttataattg gtaaagaaat tctcttaata ctaaagcgct cataatgttg
     2761 taattattaa acttttgagt gatagattta tacattttgt tgtttccctt tatatataaa
     2821 acaccatcaa gaatagccac cttgatcgct ttaacttttt taacttcaag agtagaaatg
     2881 gcatcgttaa attgcgcatt ttgatgtcct ccgaaatcag ttaaaaactt cgcttcacct
     2941 attacatact tcccattgaa tcgggcaaca aaatcaagtc ctttatcatg ataataaccc
     3001 agcttttctt tagcaaaatc cattaaaact ttatcgcccc catccaaaat cgcatcatta
     3061 ttaccggaca taaattcatc tatttcaaca ggcaaaattc ctaaagattt gtttcgcaac
     3121 caatctttga acattggccc aatttgtcga ttcgtttctt tgggctcgct acatctttca
     3181 taaagtttat tgagccctaa ttcataaatt cttccacaaa ttctgttgat ggttcttggg
     3241 ttcctatcaa tagctgaact atctcttttc aaatacgcaa tataactatc ttttatagga
     3301 aaaagatcca attttaaaag ttcttttatc agtcgataat tgtccctctt tataaaggca
     3361 ttttcaacct ctctccatat ctgcacatct atttctcgta ttccttccgg aattgttgga
     3421 tatacttgaa ataaatcatc gagatatgat ttttgattgg catattcaat actaagatta
     3481 atccatttat tcatttataa ataattttta aatatcaatt tgaaaaactt gctcatatat
     3541 ttttcccctt ccgaatacct tacaaattta agcgaaaatt ctcaattttc ataccgtcaa
     3601 aagctcgttg attttttaag catattcatt caggcaatta gtattattcg ttagattaca
     3661 aaattttatt ttaaaccgac tggcaaaaat cttataatta aattttcccc gaacaaaaga
     3721 attcttgaaa aaaattcctt ttgtttctgt aaatttacaa caattttgag gcttttgttg
     3781 ttaaagtcaa atgattaatt cgatttattt atagaaaatg aatacgttag tggcagcacc
     3841 aaaaaaaatg ggaataactc aattagttga tattgactta aaagaaattg ttccttttat
     3901 cgactggaac tttttctttt tagcatggag attacctggc aaatatgaag gaatcgaaaa
     3961 tctttgcaat tgtacctcct gtgaagtagg gtggttgcag caatttccgg aaaataatcg
     4021 taccaaagct gaagaagcac tcaaattgtt ccgcgatgct cagcaaatgc tccaggattt
     4081 tcttcatgag aagtcgatat ccattcatgc catttttggt ttctttcgtg ctgtttcaca
     4141 aggcgatgac ttattaataa gttttggcga ccaaacgtat attttgccta cacttcgcca
     4201 gcaacacgct tcatcagacg gtttttgcta ttcgttggcc gattttataa atacagaaaa
     4261 tgattatatg ggagcttttg caacgactgt agaaggtgcg gaaacgatgg ccgaaaattt
     4321 tgagagagaa gacgatttat atcgtgccat tatggttaaa acgctctccg atcgactggc
     4381 agaagctgcc gccgaatggt tgcattataa ggttagaacc gaattttggg gctatgcacc
     4441 cgacgaacca ttggagataa aaaacatatt gaaaggaaaa tatcagggta tcagaccagc
     4501 agttggttat ccttctcttc ccgatcagtc tataattttt gagttacaat ctttgttggg
     4561 actcgaaaaa attggaattc gcctgacaga aaatggagcc atgtttccca atgcttcagt
     4621 gtgtggtttg tatttttctc atccaaagtc gagatatttt atgattggga aaataaatga
     4681 tgaacaactg gaagattatg cccgaaggcg gggaaaaacg gttcaggaaa tgaaaaaatg
     4741 gctggctgca aatatttaga tttaattgat taacaacaaa aaatagctaa atataatttc
     4801 attattaaaa atttcttgaa aaggagataa aatcatgtta gaaaaatcat tgaaaatcaa
     4861 aggaatgagt tgctctcatt gtgcaactcg cgtagagaag gcacttaatt ccttagatgg
     4921 cgttgaagct aaagttgatt tggcttccaa ctcggcaaaa cttacacttg ccaaagaatt
     4981 gagcaacgaa gaaataaaaa aggctgtgga aaatgttggt tatgaagtga cagaaatttc
     5041 ggaataattt cggcttgtca aaaagtttta aaaatagggt tgcccgaaaa acggacaacc
     5101 ctatgctgcg agaatgtaaa acaaaacaga taaaaaatca aaactttaga aaatcaacag
     5161 acaaataaat tttgaatagt tttgattgtg aaaatttcat tctgcaaaga tttattcaat
     5221 caagattgac gtcaaaaaca tgttaatttt ataagaagct tgaagaatta ttcaaagata
     5281 gttcattcga aaatttttgg ctacgcagat ggtaaaattt tatcagtaaa aggtaaaatt
     5341 ttgtagcaaa tttgtttttt tatttcattt ctttagttta attttgtttc agatgaaata
     5401 aaattatgct aaaaattatt ttgttgctct ctccggttta tgttactctt ttttggtgta
     5461 tagttttaaa cactcaagga gataattatt ctgctcccaa gtcttttttg gggaagttca
     5521 tgtttttttg ttttattgta tattcatctc atttcttgta ctttatgaaa ttagaggaag
     5581 cttatagctg gatcgatcct ctatatcaat ttgcttcttt gctggtttat cccatgtatt
     5641 acatatattt tcgtttactc accgtcgaca gaaaattttc atttaaaaaa cattttgttt
     5701 atctgctgcc atccactatt ttgtttgtgc tttattcgat agctgtgttt tttactcctc
     5761 aagaagaata caaagcattt cttttcggcg aagaacatca ctcactgaat catttaagct
     5821 atattgaaat cgttagtttc ctgatacgga tagttttttt gattcaggta atattaacgg
     5881 taataggcaa ttttcgtttg attaggaaat atggacacaa ggctgcacaa tattattcca
     5941 atatggaaga cagcagcatt tccaaagtca gattactgaa ttatacgctt atcatcaccg
     6001 ctatttcttc ctttattctg ggaatgctgg gtcgtggatt tttcgcctcc gaaataactt
     6061 atctggctat agcttcagta gttttttcgt ccatgctgtt tgcaatcgga tggcttggga
     6121 ataaacaaaa atctttaaat cctgcttatg aaacagacag catcactgaa accaaacaaa
     6181 aatccgaaga actgtccctg atccgccagg aagaattact aaataaaatt gaatatttct
     6241 tcaacgacag aaaaatatat ttgaacagca gccttactat tttggatgta gcccgattaa
     6301 ccggcacgaa tcgaacgtac gtttcctcac tcattaatca tcattttcac caaaatttca
     6361 gtgcatttgt aaatagttat cgcttaaagg aactggaaga aaaaatttta tttaatccgg
     6421 agtatcccca tcagattttg gcagaaactt gtggtttcgg atcgattgat tctatgaaac
     6481 gtgccattaa tcttagatac aagctgtcgt tgcaggaatt caaatcagag gttctcctca
     6541 aaaaagtggt ttaacgggtt aaacaaacaa taaaaatact ttactattct caagcatcaa
     6601 agtttttaaa aattctcaac aatcaatcga aataatttac tgagacctaa atttttcaca
     6661 atttcatcag aatataattt attttttaaa ggtctgatgg aacccacatg tctgaatatt
     6721 atattcattc gaatttgtgt ttgcaataca tgtgggtttc atttgattaa aaacttgtta
     6781 tattttcctt ttttatgaaa aatttttgag aaagacctgg aaaaaataat taaaaacatt
     6841 tatctttgca gtgtgatttt tttcatggta ttagatttaa ggttaataag gaagattggt
     6901 ggtcaggcgt ggctaccatt cttttttata gcaatttttt tcgtattaat tgctttttca
     6961 ataaggatgc cgtacatgtt aacaaactgt tcgcctgctt taataacagc tctttttgaa
     7021 atttcttgtg catgattact gggctttcta attttattgt ttccattttc tgttattttt
     7081 agtggttatt ttgatatcat aaggataata gatatatttg ttatatagaa atttttagtg
     7141 ttaaattttc gacctgaaac tttgttttat gattaattaa cgttttgtct ttcatgtatt
     7201 tgcataaaaa atgatggaat tttgttgcaa taaaaaaagg cggtgttacc cgcctaaaca
     7261 tccgattagc cactcgcatc ccaccgcctt gtttcatgcg caaatagcct tgtccgctaa
     7321 tcagaatttc ttatattgct ccttagttaa tatcgtacct cccatgtcgg ctctggttac
     7381 aatatttgaa gtttcatcaa tcttatattc ccttcctcct ttaaattttg caaacgtctt
     7441 gtaaccgtct tttgaatagg aatatcgtat tgcgacttct tcaatatcgt ccatgatgta
     7501 aatctcattc cctttttgga agttttcgta aatgtaatct gtgttgttca tctaaatttg
     7561 ttataatatt ttgttgacaa atctattagc tcatttcttc tttttctgcc ttctttagat
     7621 attttagatg tacttctgta ttcctcataa aatttatgcc ccaatccacc ttcaagacct
     7681 gtttctttgt gtatttcatt ccaccttttc tcaccaataa ttttttttgc atcttctggt
     7741 ttttctttcg caaatatcat tcggtcggta ttaaattgaa tctctccgac gattccatct
     7801 tttgttctta catttgctaa cactcctgaa tagccttgaa atttttctgg tgtttgattt
     7861 tttacacgta taaagatagt atttttttat attatcaata atcaaccaag aaaaagattt
     7921 aattacgaat cgcctatttt tatatttaat caaaaagttg cattttctac ttgaatgttg
     7981 ccaataaaat attctgtatt tttctattta tctatttttt cagaaagtta cattacgcaa
     8041 tttttaatcg gtgaacattt gttttttcga ttttactttg agcgtaaggg agcgtaacca
     8101 cccatttggt agattttttg gatggcattt cgtacatcga atccatcatg atggtttcgg
     8161 taatggaacg cagacctctg gcgcccaatt tgaactctat agctttatcc acaatgtagt
     8221 ccaacacttc atcttcaaaa atcaattcta tgttatccat tttgaatagt ttgacatatt
     8281 gtttgataat cgaatttttt ggttcggtaa gaatgcgacg caacgcatcg cgatcgagcg
     8341 gctccagata agttagaatg ggcaaacgtc cgataatttc cggaataagg ccgaaagatt
     8401 tcaagtcttg cggagcaata tattgaagaa gattgtttct gtcgatgagt ttgttttctt
     8461 ccgaagctcc gtatccgacc actcttgtat tcaatctggc agcaattttc ttttctattc
     8521 catcaaaagc gccaccacaa ataaacaaaa tgttttgcgt gttgacggga atcattttct
     8581 gttccggatg ttttcgccca ccttgaggcg gaacattcac caccgatcct tccaacaatt
     8641 tcagcaaacc ttgctgaacg ccttctccgc ttacgtcgcg agtaatactc ggattatcac
     8701 ttttgcgggc gattttgtcg atttcgtcga taaaaacaat tcctctttcg gccgctttta
     8761 catcataatc ggcaacctgc aacaatcggg tcaaaatact ttctacatct tcgcccacat
     8821 aacctgcttc ggtgaaaacc gttgcatcaa cgatggtgaa aggtacatgc aactttttgg
     8881 caatggttcg tgccagcaac gttttacccg ttcctgtggg gccaaccatg ataatgtttg
     8941 atttttctat ttcaacttca tcttcttccg ttttctgcat caaacgttta taatgattgt
     9001 agacagccac cgaaagatat ttttttgctt cttcctgacc gataacatat tggtcgagat
     9061 actgcttaat ctcttttggt ttgggtattt tttcgatatg caaatgggaa gatttggact
     9121 ttcgggcact ttccctgaca atctcagctg cttgaatggc gcattcatta caaatttgca
     9181 cgccatcctg acctaaaatc ataaattcta cttcagcttc agagcgtccg caaaaattgc
     9241 aatgtctaac gttttttggc atacttgtaa tattattttt tactctttcg ctcgttaatc
     9301 aacactttat ctatcattcc gtattcaagt gcttcctgtg aagtcatcca ataatcgcgg
     9361 tcggcatctt tttctatttt ttcgtaagga tttcctgaat gttcggcaat aatgttgtaa
     9421 agttcctgtt tcattttttg aatttcgcgt gccgtaattt ctatatcgga agcttgtccc
     9481 tgagctccgc ccataggttg atgaatcatg atacgcgagt gtttcagggc aaaccgtttg
     9541 cctttggttc ctgctaccag cagaatggcg cccatagacg ctgccatacc cgtgcaaatg
     9601 gttgaaacat cggaactgat atactgcatg gtgtcgtaaa ttcccaatcc ggcataaacg
     9661 gatccgcccg gagtattcag gtaaagagaa atatctttcc ccggttcggc cgaatcaagg
     9721 taaagcaact gtgcttcaat aacgtttgaa gcgtaatcat cgatttcggt gcccagaaaa
     9781 ataattctgt ccatcattaa tcgggagaaa acgtccattt gtgtaacatt cagctgacgt
     9841 tcttccagaa tagtgggaga aatatagctg tcattaataa aaccggcata ctgatcgata
     9901 gctaaaccgt tcattcccag atgacgggtt gcataattgc gaaaatcgct tcttatattt
     9961 tttttgttca tattgtttcc tttcttttta atttaaggtt tttttttaat atctacctga
    10021 aaaagttctg atataaacaa attaaaaagg tttttgtttt caatgaagtg ttttcaatga
    10081 aaattttttt aattttcctc aaataatttg tcaaattctt caggactgat ttcttttacc
    10141 tctacttcaa cactatcttt tatggttaca ataactttat tgtcgcctgc gcgctcgatg
    10201 aaatttctca gggtatcttc cttttcaagc aagctcttgg catagttgga aattaattct
    10261 tcgtcggctc cgcttatacc gtactgagca agctgtgctc tggcaaattc tttggcgtaa
    10321 ttttcaactt ctacttcatt cactttgatg tcgaattttt ttatcagctt ttccttgata
    10381 agttgccaag tgaggtcttc aatcatttta gggtaatccg attccaattg ttcgttggtc
    10441 aaatttttgt tggaagccaa caaccagcgt ttcaggaaag catcagggaa agaaagattc
    10501 ttgaattttt ccaccaatgt tttcttggca tcaatgccaa atcgatagtc gctgttttct
    10561 tttaaattgg tggcgatagt ttccttgact ttttcgcgaa attcctgttc gttggttact
    10621 gcattttctc cgaaaacttt atcgaacaat tcctgattga tttcgctatc gaagtagcgg
    10681 gtaatatcgt taattttgaa actgaaatcg gaagtaattt ctttagcctg ttcctttgaa
    10741 attttcagga atgaagccag ttctgtttca ttgccgaatg ctttggccgg gttgaaggta
    10801 acggtatcgc ctttttttgc atttaaaaac aatgtttttt gttcagggtc ggcgatgtag
    10861 gctggtgtga ggatagcttc cgaaacttgc aagccgtttt gcttttcttc accgttttcc
    10921 aattcaatga cttcgccccg aagcatatcg ttttcttcta ccgtttccac ctcttcgtag
    10981 cgaccaaatc tttgactata gctttttatg cggtcttcta tcatttgatc ggttacagca
    11041 atgttgtaat aatttatttt gatctctttc gacaattcta tatcaaaatc gggagccagc
    11101 cccagatcga aaacaaaatc aaactcttcc tgagtattga aatctatttc gggctgctca
    11161 gtttcgttgg gcagtggatc gcccaatata ttcaaatcgt tttcgcgaat ataatcataa
    11221 agtttttctg caatcagctt gtttatttcg tcagccatga cagatttgcc atacattttt
    11281 ttcaaaaggc tcatcggaac cattccggga cgaaagcctg gaatattagc ttttttgcgt
    11341 aaatcgcgaa gatttttttc aacattttcg gcgtaatcgg ctttttcaac gtgcagagtt
    11401 actactgcat tgttttgatc gaggtctttt ttaacaatgt tcatactgtt agttttctga
    11461 tatttataat ttttataatg aagtgcaaag gtacaaaaaa agaggaactt ttctaaattt
    11521 tacattatta tctgacaaaa ctttcatttt atcgatattt atacttgtaa cccccataaa
    11581 cagggcttta aattattatt ttcaaaagag acctcccctc aaaattgcag gttcagggta
    11641 aattgaccta tagcaggtgg agttttttga ttcttatcaa aggttctttt tgtccccctg
    11701 aacaattcat aaatatctaa caagccgttc aaatctatcc gtatccctgt tgccactatg
    11761 gaaaaggcct ttttttgtgc cggctttctt ttgcaatact gtcaataaca gttgagctaa
    11821 cacaaggtac accatattag ggtgcgtatg gtcgtttcat atttctaaaa ttcaacttat
    11881 cgttttttct tgtaaaataa aagtattatg tttgagaagt tatttccaaa tttcaacaac
    11941 ttcgtctttt ttatttgg
//
LOCUS       DAOSCH010000062        11886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3619_length_11886_cov_5.925450, whole genome shotgun sequence.
ACCESSION   DAOSCH010000062 DAOSCH010000000
VERSION     DAOSCH010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11886
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3619_length_11886_cov_5.925450"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..2042
                     /gene="thrS"
                     /locus_tag="PLZ54_05890"
     CDS             99..2042
                     /gene="thrS"
                     /locus_tag="PLZ54_05890"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOJ66491.1"
                     /translation="MIKITFPDGSVKEFPKGITGLEIAESISSRLAQDALSITVNGQV
                     WDLTRPIETDAAVKINTWEDEEGKHAFWHTSAHLMAEALQELYPGIKFGIGPAIENGF
                     YYDVDPGEAVIRESDLPAIEAKMAELAARGEKLKRTDISKADALKKFQTKGDEYKVEL
                     INDLQDGTITLYSQGNFTDLCRGPHLPNTEKIKAIKLLSVAGAYWRGDEKRKQLTRIY
                     GITFPKKKMLDEYLLLLEEAKKRDHRKIGKELELFMFSEMVGKGLPIWLPRGTDLRLR
                     LENVLKTIQQRFEYQQVITPHIGNKQLYVTSGHYAKYGKDSFQPIHTPEEGEEYLLKP
                     MNCPHHCEIYKFKPRSYKDLPLRFAEFGTVYRYEQSGELHGLTRVRSFTQDDAHIFCR
                     PDQVKDEFLKVMDIIFIIFNALDFKNFEAQISLRDPDNKEKYIGTDENWEKAEKAIIE
                     ACEEKGLKAKIVQGEAAFYGPKLDFMVKDAIGRKWQLGTIQVDYNLPERFELEYTGED
                     NQKHRPVMIHRAPFGSMERFIAVLIEHTAGKFPLWLTPDQVVVLPISEKFNDYAFDIA
                     KKLKEKSIRVTVDERNEKIGRKIRDNELKRIPYMLIVGEKEMETSHVSVRRQGEGDAG
                     SMSVEEFAELIHNEVVSQMKQFN"
     gene            2255..3568
                     /gene="der"
                     /locus_tag="PLZ54_05895"
     CDS             2255..3568
                     /gene="der"
                     /locus_tag="PLZ54_05895"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOJ66492.1"
                     /translation="MGNIVAIVGRPNVGKSTLFNRLTQTRRAIVNETAGTTRDRQYGK
                     SEWSGYEFSVIDTGGWVTNSSDTFEQEIKRQVQLAINEADVILFLVDVQCGITDLDFE
                     VAQLLKKTNKPVILVTNKADTFEWQYQAPEFYKLGMGDPFLVSSINGLGTGDLLDKLV
                     SYLKPQEDEETEEHLPRFAIVGRPNAGKSSLLNSFLDEERNIVTEIPGTTRDSIYTRF
                     NKFGFNFYLVDTAGIRKKAKVDEDLEYYSIVRSIRAIENSDVCMLLIDATRGLESQDL
                     NIFSIIQKNKKGIVVCVNKWDLIEDKDTHSTKKMEAYIRERMSPFTDFPIIFISALTR
                     QRIFKVLESAVKVYENKNKKIPTAKLNEYLLPLIQNFPPPALKGKYIKIKYVTQLPDT
                     QVPTFIFFANLPQYIKEPYRRFLENKIRSQWNFTGTPIQLFFRKK"
     gene            complement(3582..4952)
                     /locus_tag="PLZ54_05900"
     CDS             complement(3582..4952)
                     /locus_tag="PLZ54_05900"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HOJ66493.1"
                     /translation="MHFNKFFLFLFTLAFVSCTDGLTDIGVSILPSQDIISVNADAFD
                     LNTENVFVDFIYTRQDSFLLGNFYDHKYGSTRADILAQVNCPVDFTFPDNAVPDSAVI
                     ALRYLTWFGDKYSPMEIKIYQMNKATFSYSEHYPSNINPDDYTDRSILLGKRIITPSD
                     TSGSRSDGTQVRFKLSDSFVQWFFPENRTYNSQKDFLQKFKGMFITTTFGSANILYVN
                     QIDLYYYYHYTYVRKTSDGSDSTVTVNNVITFPANAEVRQVNRIQHPDRNEVVQSRPE
                     VNYVASPANINTRISLPVDKIAKQMKTTIHNKYLSVNSALLKVEVTEQDEATIAVPLV
                     QNMMLIKESALDRFFKNSELPSDTCALIAQYSYTKNADTNSYDYYYTFNLSKLIANEI
                     KKAETNGTQLPETMDLLLVPVTVQYNNSGYYTSVKHQILLSGVTIKSGKNTSSPMKIN
                     LVYSGF"
     gene            complement(4983..5795)
                     /locus_tag="PLZ54_05905"
     CDS             complement(4983..5795)
                     /locus_tag="PLZ54_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004301787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOJ66494.1"
                     /translation="MKEVTKVLYISQEIFPYLPASEMANMGRYLPQAVQEKGLEVRIF
                     MPKFGSINERRNQLHEVIRLSGMNIIIDDTDHPLIIKVASIPAARMQVYFIDNEDYFQ
                     RKNTVANENNEEYDDNDERAIFFARGVMETVKKLRWSPDVIHCQGWITALVPLYVKKI
                     YNDDPFFKHSRVVYSIFDDEFKKPLNDRLMDKILMDGIQKKHLASIKNPPVDYHSLLK
                     LAADYSDGFIQSSPKVNESVLKYVADKKIKFLPYQPAEDYSDQYIDFYNQLF"
     gene            6304..6660
                     /locus_tag="PLZ54_05910"
     CDS             6304..6660
                     /locus_tag="PLZ54_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HOJ66495.1"
                     /translation="MPLIYSYLMDWKEKLNQLKDSLPQDNSEPTENELPEKKVQTEPL
                     RVEMEKRRGKPVTIVSEFEGNEKELKELAKTLKVSCSAGGTAEHGMVLIQGDFRVKIA
                     EILQKMGYKVKKINFK"
     gene            6700..9987
                     /locus_tag="PLZ54_05915"
     CDS             6700..9987
                     /locus_tag="PLZ54_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOJ66496.1"
                     /translation="MLNVYRASAGSGKTFQLTKDYIHLLFRHQGENLHRHILAVTFTN
                     KATEEMKSRILKELFALAQGQPSNYRKSLMEEYARNEAEVNLKAKQILVSLLYDYSSF
                     AVSTIDSFFQQVIRSFAREMGINGAYNLEMENDAILSQAVDNLFFNLSKNENKLLLDW
                     LISFAEESMEQNGKWNLKNDIINFGAEIFKENYQYRAEEIGKKLHNLDFLGKYIGKLK
                     KIKTDFEEEVKKLAQDGIRIMTDFELSHDDFSYKETKLFDKLISGNVAAPNSRFRKYA
                     DDVENCTNKSTPNWVKNKVNSAYISGLGKCFEKINEIFQEPFIRYNSVDIVLKHIYKL
                     GILSDLAVEINQLTAEQNTFLISDTNLLLNRIIDNSDTPYVYEKIGNKLQHFMIDEFQ
                     DTSVLQWKNFLPLVRNSLAENNFNMVVGDVKQSIYRWRNSDWKLLDEQIFKDFTPQQI
                     RQEPLNVNWRSDRNIVKFNNNFFSQSAQLLQQQLNANIQEYLPADKYPFVSELSNKIQ
                     HAYTDTEQKVSEKAGAGHVCIQFIPNDEKNWQEESLNRLPIMLENFQDRGYKASDIAI
                     LVREKKDAALVIQSLMNYKNSPSAKEGYCYDILGNEGLLIGKASSVRFIVGLLKLFVS
                     PSDDIQLTIVSLEYALNKLGKTKNEALRDIAALKENTENKFSHLFDSEEEKALQAIRH
                     YSLFDRVEQIISLFNIGEWENQAPFVQAFQDLVYNFTAKGFSDISDFLSWWDEAGCNK
                     FVAMPADQEAIHIMTIHKAKGLDFPVVILPLCDWALEGNIHKTNVLWCETNVPPFSEL
                     PLLPISYNQYLRQSIFTQNYFDEQMHLYIDNLNLAYVAFTRPKNELVCMAPAPDEKNE
                     EKKKKSTSETVKNVSSLLWQFVQTQQSTIGENSSMRKDDENKIYEWGEATYAPQKEII
                     SSTKSEKFVQYPSCNSAQKLQIRHQGTQYLIEEKDLSDSQLNYGIIMHEILRKITNQA
                     DQDKVLLEFVSSGQISQAEKITVEKGLEKFWSLPQAKEWFNGEGRILNETTILSPENR
                     LHRPDRVIIQEAKATVIDYKFGQKESKYHLAQVKEYMDLIAQMGYQTKGYVCYAELGK
                     VVEVFQN"
     gene            complement(10035..11063)
                     /locus_tag="PLZ54_05920"
     CDS             complement(10035..11063)
                     /locus_tag="PLZ54_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ66497.1"
                     /translation="MFAFKVIHAAFYFFADMKVLVTGATGLLGHQVTMELIQHGYQVQ
                     IIVRKMDNIFFDLNAVKVFIGNFTEYEQLKAAASGCEAVIHIAAVTDVHLLHYEDYRK
                     INVEGSAQVIRVCRDLNIRNVVYISSSNTIGFGSKSKPATEDFPFQYPFTRSFYARSK
                     VEAEKLFQQFASEFGNHVIIIHPTFMIGAYDVKPSSGKLLLMGYRKLLLFIPKGGKNF
                     VPVRDVAKAVCNALTLGKNGEHYLTAGVNLSFAEYYRLQKNIAGYRQIIIKVPDFLLK
                     VLGKVGDFLRFLGIKTDVCSMNLSQLMIREYYCGKKAIDELNMPQTDLKLAIAEAVEW
                     FKERGWIH"
     gene            complement(11117..11572)
                     /locus_tag="PLZ54_05925"
     CDS             complement(11117..11572)
                     /locus_tag="PLZ54_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOJ66498.1"
                     /translation="MEKIDELDRQILKIITQNARLPFRDVAVQCGVSRAAIHQRVQRL
                     IDMNVITGSGYFVNPKKLGYQMCVYIGITLERGSMYKEVVQELEKIPEIVESQYTLGP
                     YTILVKLYAKNDEHLMELLNGKIQEIKGVATTETLTSLDQRIRRTIPID"
BASE COUNT         3945 a         2338 c         2325 g         3278 t
ORIGIN      
        1 gcatccgaag cctcctcttt ccgaataagt catcgggctt tgaagaaaaa ccattatctt
       61 tgcgaaaaat ttaaaaacca tttgtaaggt tcatgaacat gataaaaata acatttcctg
      121 atggttcggt aaaggaattt cccaaaggaa ttaccggact tgaaatagct gaaagcatca
      181 gctccagatt ggctcaggat gccttgtcga taaccgtaaa cggtcaggtg tgggacttga
      241 cgcgccccat cgaaacggat gctgccgtaa aaatcaatac ttgggaagat gaagaaggaa
      301 aacatgcatt ttggcacact tcggctcact taatggcgga agcattgcag gaattgtatc
      361 ccggaataaa attcggtatt ggtccggcca ttgaaaacgg attctactac gacgttgatc
      421 cgggagaagc ggtcattcgc gaaagcgatt tacccgcaat tgaagcaaaa atggccgaat
      481 tggctgcacg cggcgaaaaa ctgaaaagaa cggacatcag caaagccgat gcactgaaaa
      541 aatttcaaac caaaggcgac gaatacaaag tagaattaat caacgatttg caggacggca
      601 ccataactct ttacagtcag ggaaatttta ccgatttgtg tcgggggcct catttgccca
      661 atacggaaaa aatcaaagcc atcaaactgt tgagtgtggc cggtgcttac tggcgcggcg
      721 acgaaaagcg caagcaactc actcgaattt atgggattac atttcccaag aaaaaaatgc
      781 tggatgaata ccttctgttg ttggaagaag ccaaaaaacg cgaccatcgg aaaattggca
      841 aggaactcga attgttcatg ttttccgaaa tggttggcaa agggcttccc atttggttgc
      901 ctcgcggtac agatttacgc ctcaggcttg aaaatgtact caaaacaatt caacagcgat
      961 ttgaatatca acaagtcatc actcctcaca ttggcaacaa gcaactttat gtaacttccg
     1021 gtcattatgc caaatacgga aaagactctt ttcagcccat tcatactccc gaagaaggag
     1081 aagaatattt gctcaaaccg atgaactgcc cgcatcattg cgaaatttac aaatttaaac
     1141 cgcgttccta caaggatttg ccactgcgtt ttgcagaatt tggtacggtt taccgctacg
     1201 aacaaagcgg cgaacttcac ggattgacac gcgtcagaag ttttacgcag gatgatgccc
     1261 atattttctg ccgtcccgat caggtgaaag atgaattttt gaaagtgatg gacatcattt
     1321 tcattatttt caatgcactc gattttaaaa attttgaagc gcagatttcc ctgcgcgatc
     1381 cggacaataa ggaaaaatat atcggcacgg acgaaaattg ggaaaaagcc gaaaaagcca
     1441 ttattgaagc ctgcgaagaa aaagggctga aagccaaaat tgttcaggga gaagccgctt
     1501 tttacggccc caagttggac tttatggtaa aagatgccat tggcagaaaa tggcagcttg
     1561 gcaccattca ggtggactat aacctgccgg aacgctttga gctggaatat accggtgaag
     1621 acaaccagaa acacaggccg gtgatgattc accgagcacc ttttggttcg atggagcgat
     1681 ttattgccgt tttgattgaa catacggcag gaaaatttcc gctgtggctt acccccgatc
     1741 aggtagtagt gctgcccatc agtgaaaaat tcaacgatta tgctttcgat attgccaaaa
     1801 aactgaaaga aaaatccatt cgggttaccg tagatgaaag aaacgaaaaa attggtcgaa
     1861 aaattcggga caacgaactg aaacgtattc cttacatgct tattgtggga gaaaaggaaa
     1921 tggaaacttc acacgtttcg gtacgccgac agggagaagg cgatgcaggg agcatgagcg
     1981 tggaagaatt tgcggaattg atccacaacg aggtggtttc acaaatgaag cagttcaatt
     2041 aacagacaaa ggggttgaat attcttttac gccgctattt tggcaattaa aaaaattggc
     2101 ttcagtaccc tgcttttctg agcccaaaaa aacacgacca cagcaaagtc aattgtttta
     2161 cttaactttg catcgaaatt aaaaaatatt ttcacccaca atcggaatat gggaatactc
     2221 cgattgaaga tttacaaatg aaaaataaaa aattatggga aatattgtag ccattgtagg
     2281 gcgtccaaat gttggaaaat caactctttt taaccggctt acgcaaacgc ggagagccat
     2341 tgtgaacgaa acagccggaa ctacgcgcga tcgccagtac ggcaaaagcg aatggtcggg
     2401 atacgaattt tcggtgatcg acaccggcgg ttgggtaacc aactcttccg atacctttga
     2461 acaggaaatc aaacgtcagg ttcaactggc catcaacgaa gccgacgtaa ttcttttttt
     2521 ggttgatgta cagtgcggca ttaccgactt ggactttgaa gttgcgcaac ttctcaaaaa
     2581 aaccaacaaa ccggttattc tggttaccaa taaagccgat acattcgaat ggcagtatca
     2641 ggcaccggaa ttttacaaac tgggcatggg tgatccgttt ttggtttctt ccatcaacgg
     2701 gcttggaacc ggagatttgc tcgacaaact ggtaagttat ttgaaacccc aagaggatga
     2761 agaaacggaa gagcatctgc caagatttgc cattgtagga agacccaatg caggaaaatc
     2821 ctcacttctc aactcttttt tggacgagga aagaaatatt gtaaccgaaa ttccgggcac
     2881 tacgcgggat tctatttata cccgtttcaa taaattcgga ttcaattttt atttggtcga
     2941 tacggccggc attcgcaaaa aagccaaagt agatgaagat ttggaatact attccatcgt
     3001 gcgttccatc cgcgctattg aaaacagcga cgtctgtatg ctgctgattg atgcaaccag
     3061 aggtttggaa agtcaggatc tcaacatctt ctccattatt cagaaaaaca aaaagggaat
     3121 agtggtttgt gtaaacaaat gggatttgat agaagacaaa gacacccatt ccactaaaaa
     3181 aatggaagcg tatattcggg aaaggatgtc tccttttacc gatttcccga ttatttttat
     3241 ttcggcactc acccgtcaga gaattttcaa ggtgctcgaa tcggcagtaa aagtttacga
     3301 aaacaaaaac aaaaaaattc caactgccaa gttgaacgag tatttgcttc ctttgattca
     3361 gaattttcct ccgccggcac tgaaagggaa atacatcaag ataaaatatg ttacgcaact
     3421 gcctgatacg caagttccca catttatctt ttttgccaat ttgcctcaat acatcaagga
     3481 accttatcgg cgatttctgg aaaataaaat cagatcgcag tggaatttta ccggaacacc
     3541 catccaactg tttttcagaa aaaaataaaa tttacagtga tttaaaaacc actgtacacc
     3601 aaattgattt tcattgggga agaagtattt ttcccgcttt tgatcgttac tccactcagt
     3661 aaaatctgat gtttcacact ggtgtagtac ccactgttgt tatattgcac ggtaacggga
     3721 accagcaaca agtccatagt ttcgggaagt tgggtaccat tggtttcagc cttttttatt
     3781 tcattcgcaa tcaatttgga aaggttgaaa gtgtaataat aatcgtaact attggtatcg
     3841 gcattttttg tataggaata ctgagctatc aatgcacagg tatcggaagg caattcgctg
     3901 tttttgaaaa atctgtccaa agccgattct tttatcaaca tcatgttttg caccagggga
     3961 acggcaatag ttgcttcatc ctgttcggta acctctactt tcaacaacgc actgttcacg
     4021 gaaagatatt tgttgtggat ggttgttttc atttgcttcg ctatcttatc gacaggcaac
     4081 gaaattcgtg tgttgatatt tgccggagag gcaacataat tcacctctgg acgcgattga
     4141 acgacttcat tcctgtcagg atgctgaata cggttaacct gacgaacttc ggcattggca
     4201 ggaaaagtaa taacattatt gactgtaacc gtcgagtcgg aaccatcgga agtctttcgt
     4261 acataagtat aatgatagta atagtacaaa tcgatttgat tcacataaag aatgtttgcg
     4321 ctgccaaaag ttgtggtaat aaacatccct ttgaacttct gaagaaaatc cttttgactg
     4381 ttgtaggtcc gattttcggg gaaaaaccat tgaacaaaac tatcggaaag cttgaaacgt
     4441 acttgtgtcc catcggaacg cgaaccggaa gtatcgcttg gcgtgataat tctttttccc
     4501 aaaagaatag accgatcggt ataatcatcc ggattgatat tggagggata atgctccgaa
     4561 taggaaaaag tagccttatt catttgataa attttgattt ccattggaga atatttatct
     4621 ccaaaccagg tgagataacg caaagctatt acagccgaat cgggaacagc attatcggga
     4681 aaagtaaaat cgacagggca gtttacttgt gccagaatat ctgcgcgggt agaaccgtat
     4741 ttatggtcgt aaaaatttcc caacaagaaa gaatcctgac gcgtgtaaat aaaatcaaca
     4801 aaaacatttt cggtgttcaa atcaaatgca tcggcgttaa cactgataat atcctgtgaa
     4861 ggcagtatgc tgacgcctat gtcggtcaat ccatccgtac acgaaacaaa agccagtgta
     4921 aaaagaaaca ggaaaaattt gttgaaatgc atattgaata tatgattatc gatatgtaaa
     4981 ctttagaaaa gctgattgta aaaatcaata tattgatcgg aataatcttc ggccggctga
     5041 taaggcaaaa acttaatctt tttgtcggca acatacttca acaccgattc attgactttg
     5101 ggactggatt ggataaagcc atcggaataa tcggcagcaa gtttaagaag tgaatggtaa
     5161 tcgacaggtg gattttttat ggaagccaaa tgtttcttct gaatgccatc cattaaaatc
     5221 ttgtccatta agcgatcgtt aaggggcttt ttaaattcgt catcgaaaat ggaataaacc
     5281 actcttgaat gcttgaaaaa cggatcgtcg ttataaattt tttttacata caaaggcacc
     5341 aaagccgtta tccaaccctg acaatggatt acatcgggcg accagcgcag tttttttact
     5401 gtttccatca cgccacgcgc aaaaaatata gcccgctcgt cattatcatc atactcttcg
     5461 ttattttcat tggccaccgt atttttccgc tgaaaataat cttcattgtc gatgaaatat
     5521 acctgcattc gggcagcagg aatagaggct accttgatga tcagcggatg gtcggtatca
     5581 tcaataatga tattcattcc tgaaagtctg attacttcgt gaagttgatt tcttcgttca
     5641 ttaatagatc cgaatttggg catgaaaatt cgtacttcca atcctttttc ctgtactgcc
     5701 tgaggaagat atcttcccat atttgccatt tccgatgcag gcagataagg aaaaatttcc
     5761 tgggaaatat acaatacttt tgtaacttct ttcatttttt ccctttatag tgagagtatt
     5821 ttcaatatgc aaagatatta aaaaaaaatg ggatacctgc aagttttggt tcccaaagtg
     5881 aagttaagct ataaaatgcc aaaaaaaaac tacagaaaaa ttgcaaaaaa taacggaatt
     5941 tatttggtcc ccggtagaaa aactttactg acataaacta agaatagaat ctgcggatta
     6001 aaaaattaat cgcagttatt ttgataaaaa tttggagccc tattattcgg ataacagagc
     6061 ttcgagtgta ccgatacgca tgatgatgga gtacctcgca agagcaggct ttctaaaatt
     6121 catcggacat gcgtcaaaag atttccgaat gaaatagaat ttttttccta taaaaagaca
     6181 gcattaaatg aatgcttttt tgttggcttc taccttgaat ttgaagaaga tgtgcgaaaa
     6241 gctgaaagaa tattttcttc atttcatttc tctattgctc tttctacaaa aaaaattatt
     6301 aacttgccgc ttatttatag ttacctcatg gactggaaag aaaaactcaa tcaactgaaa
     6361 gacagtttgc cacaagataa tagtgaaccg acagaaaatg aactgccgga gaaaaaagtg
     6421 caaacagaac ctttacgcgt agaaatggaa aaacgcaggg gaaaaccggt tactattgtt
     6481 tccgaatttg agggaaacga aaaggaactg aaagagcttg caaaaacact aaaagtttca
     6541 tgcagcgcag gaggcacggc agaacacgga atggtactca tacaaggcga tttccgtgtg
     6601 aaaattgcag aaatactcca aaaaatgggt tacaaagtca aaaaaatcaa tttcaagtaa
     6661 aaatcgttta ttttcatcac actgtaaaag aaatttttta tgttgaatgt ttatcgggct
     6721 tctgctgggt cgggaaaaac tttccaactc acaaaagatt acattcattt gcttttccgc
     6781 catcaaggtg aaaatcttca tcgtcatatt cttgcggtaa cctttaccaa caaagctacc
     6841 gaagaaatga aatcacggat tctgaaggaa ctttttgcgc tggcacaagg gcaacccagc
     6901 aattaccgaa aaagtttaat ggaagaatac gctcgcaatg aagcagaggt caatctcaaa
     6961 gccaaacaaa tactcgtaag tctgttgtac gattactctt catttgctgt cagcacaatt
     7021 gacagtttct ttcagcaggt tattcgcagt tttgcacgtg agatgggtat caatggagcc
     7081 tacaacctcg aaatggaaaa cgacgcgata ctttctcaag ctgttgacaa tctgtttttc
     7141 aacctatcaa aaaatgaaaa caaactgtta ttggattggc tgatcagctt tgccgaagaa
     7201 agcatggaac aaaacgggaa atggaatctt aaaaacgata ttattaactt cggtgctgaa
     7261 attttcaagg aaaactatca atacagagcg gaggaaattg gcaaaaaact tcacaatctt
     7321 gattttctag gaaaatatat cggcaaactg aaaaaaatca aaacagactt tgaagaggaa
     7381 gtaaaaaaat tggcacagga cggcattcgt ataatgaccg attttgaact gtcacatgat
     7441 gattttagtt ataaggaaac aaaattattc gataagctga tcagtggaaa tgtagctgct
     7501 cccaacagcc gtttcagaaa atatgcagat gatgtagaga actgtaccaa taaaagcaca
     7561 cccaattggg taaaaaataa agtcaactca gcctatataa gcggattggg aaaatgtttt
     7621 gaaaaaatta acgagatttt tcaagagccc tttatccgct acaattccgt cgacatagta
     7681 ttgaagcaca tttacaaact gggtattctt tctgatttgg ctgtggaaat caatcagttg
     7741 actgccgaac aaaacacatt tttaatttcc gacaccaatc ttttgctcaa tcgtattatc
     7801 gacaatagcg atacgcctta cgtttacgaa aaaatcggca acaagctgca gcattttatg
     7861 attgacgaat ttcaggacac atcggtactt cagtggaaaa attttctccc gcttgtgcga
     7921 aacagtttgg cagaaaataa tttcaacatg gttgtgggag acgtgaagca aagtatttac
     7981 cgctggagaa attccgattg gaaattgttg gacgaacaaa ttttcaagga ttttactccg
     8041 caacaaattc gccaggaacc actcaacgtc aattggcgca gcgacaggaa tatcgtgaaa
     8101 tttaacaata attttttttc tcaatctgct cagcttctgc aacaacagct gaatgccaac
     8161 attcaggaat atttgccggc cgacaaatat ccatttgtgt ccgaactatc caacaaaatt
     8221 caacatgcct atacagacac cgaacagaaa gtttcagaaa aagccggagc tgggcatgtg
     8281 tgcatccaat ttattcccaa cgacgaaaaa aactggcaag aagaaagttt gaaccgcttg
     8341 ccaatcatgc tcgaaaattt tcaggatcgt ggttacaaag cttccgatat tgccatattg
     8401 gttcgcgaaa aaaaagacgc agcccttgtc attcaaagtt taatgaacta caaaaactcg
     8461 ccctcagcca aagaaggtta ttgttacgat atattgggaa atgaaggatt gctcataggg
     8521 aaagcatctt cggtacgttt tatcgtcgga ctgcttaaac tttttgtatc tccttccgac
     8581 gacattcaac tgacaatcgt aagcctcgaa tatgcgttga ataaattggg aaaaacaaag
     8641 aatgaagcct tacgggacat tgctgcatta aaggaaaata ccgaaaataa attctcccat
     8701 ctatttgatt ccgaagaaga aaaagcacta caggcaattc gtcattattc gctttttgac
     8761 cgcgtagaac aaattatttc ccttttcaat ataggtgaat gggaaaatca ggcgccattt
     8821 gttcaagctt ttcaggattt ggtatataat tttacagcca aaggtttttc cgatattagc
     8881 gattttctat cttggtggga cgaagcgggc tgcaataaat ttgtagccat gccggccgat
     8941 caggaagcta tccacattat gaccattcac aaggcaaaag ggctcgattt tccggtcgtt
     9001 attcttcctt tatgcgattg ggcattggaa ggcaatatac acaaaaccaa tgtgctgtgg
     9061 tgcgaaacaa acgttccgcc tttcagtgaa ttgccgctgc tgcccatttc ctacaatcaa
     9121 tatttgagac agagcatttt tactcaaaat tatttcgatg agcaaatgca cttatatatc
     9181 gataatctga atcttgctta cgttgcattc actcgtccaa aaaacgaact tgtttgcatg
     9241 gcgccggcac ccgatgaaaa aaatgaagaa aaaaagaaaa aatctacgtc tgaaactgta
     9301 aaaaatgttt cttccttact ttggcaattt gttcaaacac aacaaagcac tattggagaa
     9361 aattcgtcga tgcgaaagga tgatgaaaat aaaatttacg aatggggaga agcaacgtat
     9421 gcacctcaaa aggaaataat ttcatccaca aaatctgaaa agtttgttca atatccttcg
     9481 tgcaacagtg cacaaaagct ccaaattcgg catcaaggaa ctcaatatct catcgaagaa
     9541 aaagatttgt ccgacagtca gctgaactac ggcatcatca tgcacgaaat tctacggaaa
     9601 attaccaacc aagcagatca ggataaagtg ttgctcgaat ttgtatcttc cggtcaaatt
     9661 agccaagcag aaaaaataac ggttgaaaag ggattggaaa aattctggtc gcttccacaa
     9721 gcaaaagaat ggttcaacgg tgaaggcaga atactgaacg aaacaaccat tctttcaccc
     9781 gaaaatcgtc tgcatcgtcc ggatcgagtg atcattcaag aagcaaaagc tactgtgatt
     9841 gattacaaat tcggtcagaa agaaagtaaa taccatcttg cacaagtgaa agaatacatg
     9901 gatttaattg ctcaaatggg ttatcaaaca aaaggttacg tttgttatgc agaactagga
     9961 aaagtggttg aagtttttca gaactgacaa aagaaaactt tttcgcccac aaaaaaattg
    10021 agaattctct cctttcagtg tatccaacct ctttctttga accattcgac agcttcagca
    10081 atagcaagtt tcaaatcggt ctgaggcatg ttgagctcgt ctatggcttt ttttccgcaa
    10141 taatactccc gaatcatcaa ctgagaaaga ttcatggaac aaacatccgt tttgatacct
    10201 aaaaaacgaa gaaaatctcc gacttttccc aacactttca acaaaaaatc agggactttt
    10261 attatgatct ggcggtaacc ggctatattt ttctgcaaac gataatattc tgcgaatgaa
    10321 agattaacgc cggcagtcaa ataatgttcc ccatttttac ccaatgtgag agcattgcat
    10381 acagccttag ccacatcgcg aaccggaaca aaatttttcc ctcccttcgg aataaacaag
    10441 agtagctttc gataacccat cagcagcaat ttccctgaac tcggctttac atcataagct
    10501 ccaatcataa atgtgggatg aataatgatt acatgattac caaattccga agcaaattgc
    10561 tggaatagct tttcagcttc aactttactt ctggcgtaaa aagagcgggt aaaaggatat
    10621 tgaaaaggaa aatcttcagt ggcaggtttg ctttttgaac caaaaccgat ggtattggaa
    10681 gaactgatat acaccacatt gcgaatattc aaatctctgc aaacacgaat tacctgtgcc
    10741 gaaccttcga catttatttt ccgataatct tcataatgca gtaaatgcac atcggtaact
    10801 gctgcgatat gtatcacggc ttcacacccc gaagccgcag cttttaattg ctcgtattct
    10861 gtaaaattcc cgataaaaac cttaactgca ttcagatcga aaaaaatatt atccattttt
    10921 cggacaataa tttgcacttg ataaccatgc tgaatcaatt ccatagttac ctgatgtccc
    10981 aacaaaccgg ttgctcccgt taccaaaact ttcatgtcgg caaaaaaata aaaagcagca
    11041 tgaatcacct taaaagcaaa catgctgcaa aaataaaaat aggttgattg actttaaagc
    11101 agtttccccc gatcgttcaa tctataggaa ttgtacgtct aatccgttga tcgagtgaag
    11161 tgagcgtttc ggtagttgca actcccttta tttcttgaat ttttccgttt agcaattcca
    11221 ttaaatgttc atcattcttt gcatacagtt ttaccaaaat ggtgtacggc cctaacgtgt
    11281 actgcgattc gacaatttca ggaatttttt ccagttcctg aaccacttct ttatacatgg
    11341 agccgcgttc aagagtaatc ccgatataaa cacacatttg ataaccgagt tttttgggat
    11401 tgacaaaata tcccgaaccg gtaatgacat tcatatctat cagcctttgt acgcgctgat
    11461 gtatggctgc tcgtgaaact ccacattgaa cagctacatc gcgaaagggc aaccgcgcat
    11521 tttgcgtaat tatctttaaa atttgcctgt ctaactcatc aattttttcc atggctataa
    11581 aattttattg tatcatttag acaacaaaaa taaatagaaa aactcgaatt ctgaaaaatt
    11641 gtcggcaaaa tttatctcaa gaattaaaaa taatatcctt ttaatagaaa atacaaactt
    11701 tttgacaaaa ggaagtcttt tcaatgatta aaatatcggt aataatttca ggaaaatccg
    11761 gaaattcagg aaaatttaaa aaagagtggc aactttcaag agttcacaac gtatttgttt
    11821 tcccggagaa aaaaatctaa ttgtacatca cttttttcat ttcatcagaa tataatttat
    11881 ttttta
//
LOCUS       DAOSCH010000063        11874 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3625_length_11874_cov_6.089686, whole genome shotgun sequence.
ACCESSION   DAOSCH010000063 DAOSCH010000000
VERSION     DAOSCH010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11874)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3625_length_11874_cov_6.089686"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..529
                     /locus_tag="PLZ54_05930"
     CDS             248..529
                     /locus_tag="PLZ54_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family transporter"
                     /protein_id="HOJ66499.1"
                     /translation="MNSLQIVGSLASLLSILNQFPQAIKVFKTKDTHSISLTMYCIVV
                     VCITLWLVYGIMLKDGPLIWANGLSLFPITYIFIVKLTNTIKGKDKLSI"
     gene            complement(1368..1787)
                     /locus_tag="PLZ54_05935"
     CDS             complement(1368..1787)
                     /locus_tag="PLZ54_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOJ66500.1"
                     /translation="MYYSIAYSQQYLIQHSIKPSVQRTAVMDYLLNNRTHPTVDEIFS
                     VLSPSMPTLSKTTVYNTLKIFIERGAARMLTIDEKNARYDVDTTPHAHFICQQCGKVS
                     DVFDVRPEVFQLPLNDNFKVNSVEIFYYGVCNNCSSK"
     gene            complement(1961..2902)
                     /locus_tag="PLZ54_05940"
     CDS             complement(1961..2902)
                     /locus_tag="PLZ54_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOJ66501.1"
                     /translation="MSKVSVIGAGNVGATCVNVLSFYQIADEVVMLDVKQGIAEGKAL
                     DINQTATLLHFNTRISGCTNDYEKTANSDVVVITSGVPRKPGMTREELVDVNAGIIKS
                     VVENVLKYSPNAILVIVSNPMDTMTYLTYKIAGLPRNRVIGMGGTLDTARFKCYLGKA
                     LNANQNEIEGFVIGAHGDTSMIPLVRYAAYKCRPVTDLLDKETLDKVVSDTMLGGATI
                     TSLLGTSAWYAPGAAAAFLVEAIIHDEKKIIPCSVYLEGEYGQNDICIGVPVLIGRNG
                     VEEILDYHLNSEEKELFIKSSESLRATNAMLKDLGVI"
     gene            3209..4348
                     /locus_tag="PLZ54_05945"
     CDS             3209..4348
                     /locus_tag="PLZ54_05945"
                     /inference="COORDINATES: protein motif:HMM:NF025273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HOJ66502.1"
                     /translation="MKNYFYSLVIIILLIGCQNKNSFTVEGNISSAKGEKLYIEHMGL
                     LKTTSVDSVKLGKNGKFHFTIKRPEYPDFYRLRLNDKLIIFAVDSTEKITIKASKEDL
                     PYNYTLTGSEQSLKIQQLRKSVANIQNKINKFSSNLAPEEQNQLMKEIEKDIENHKRE
                     AQKLILENPRSTAAYYALYQKVNDFYLFSPYVKEDRSYCAAVATAYHTFMPNYTRSQN
                     LYATVMDAIKNERKAESQLRLEELFRTNSTGYINIVLPDKNGIQQKLSDLEGKIVLID
                     FSIYTARENVEHTFTLRELYNKYHERGFEIFQVSLDENRFLWEKYVQNIPWICVRDER
                     GPNTPYVLSYNISTIPASFLMDRQGNIIARDLDFKQLDKQIAKLL"
     gene            4424..5362
                     /gene="queG"
                     /locus_tag="PLZ54_05950"
     CDS             4424..5362
                     /gene="queG"
                     /locus_tag="PLZ54_05950"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOJ66503.1"
                     /translation="MRNFIINTALSIGFDACGITKAETLSADASYLQSWLKAGFHGSM
                     KYLERNFDKRVNPKLLVPGCESIIVVLMNYFPSEMQLSAAPQIAKYAYSKIDYHKVLK
                     TKLQQLEKAITEKFGKNSFNDEVQHIFVDSAPVLERRWAEKAGLGWIGKNKQLIHPGL
                     GSYVFIGILMMNAETEYDLPIKNRCGNCRKCIDSCPTQALSEEWLDANKCISYLTIEC
                     KTNIPEEFQGKLSNYAVGCDICADVCPWNRKWAQPNRHPELETASPIKKWNCKQWENL
                     NQEEFESAFSKSAIEYVGYSKLKENISILMEKFKKD"
     gene            complement(5386..7773)
                     /locus_tag="PLZ54_05955"
     CDS             complement(5386..7773)
                     /locus_tag="PLZ54_05955"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOJ66504.1"
                     /translation="MENNIDSRVAANENEVIDLRQLLIKYLSKWHWFVISIVFCFIVA
                     FLYLKITHNKYQVQTTILLRNQNSTPELTNMTMFSNMGLLGVSKEVEDEIQVLSSKTL
                     VRQVIDTLNLGTEYYVKNGLRYDEVYPISPVQIFVSNNFNDTLKQKIILILSPTSKGY
                     DISFQYGEIIEKKYRVSDLSTPLSTPIGSIRFFVNGPLKSGQKIKIISYPLAALTNQL
                     AASISVATVNKNSNAISISTVSPTPHKAKVMLDKLVELYNLDAIFDKNMILTNTADFI
                     ESRLRLISSELLDVELNVENYKKTHGLTDISSEAQLYLETASEYEKKLADIETQLNLV
                     NYVESYINESKNQFNLIPANLGIKDPALQKLIEEYNVALLERMKILRSSNEQNPVITQ
                     MEQQINAIRSNIVKSIAGVKDGLIISKNDLMAQNNEFTSKIKSVPTQERQFMEIKRQQ
                     QIKESLYVFLLQKREENALNLASTIPSARTIDAAYTTGSPISPKRMIIFLLALILGLG
                     LPILVMYLMEFFNNTVQDRKEFQKFVKAPFLGTIGLSKETENIVVKEGRTTPIVEMFR
                     LVRSNLQFMFSDIKSPVILVTSTISGEGKTFIATNLAMSFALMKKKVILVGVDIRNPM
                     LAEYFNLKKNNKGLTIFLADPNCQMDEIIQPSEVHPYLDVILAGPVPPNPAELLMSQR
                     LDELIRELKFRYDYIILDSAPIGIVSDTFQLNRLVDNTICVARLKYTPKEALHFINHT
                     YESKKLKNMATVLNGTNDVSHYGYGYKNEKKYKYRYRETTQNALEKRLNKLKKNK"
     gene            complement(7811..8596)
                     /locus_tag="PLZ54_05960"
     CDS             complement(7811..8596)
                     /locus_tag="PLZ54_05960"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOJ66505.1"
                     /translation="MRNFSFLFFTLLIVLFTGCTSQKKIAYFYGVNEKSVANINQSFT
                     KGHEARICTGDMLSITVSGLDPEAVAPFNLPLVSYANPGSTQIYASPVLQSYLVDING
                     YINFPIIGPIKLVGLTKSEAIAYISELLSPYLKNAIITIQFLNYKVTVLGEVLRPGQY
                     SINNERVTVLDALGLAGDMTVYGKRENVLLIRENFGKLEFVRLNLNSEDVFTSPYYYL
                     QQNDILYVEPNVVRSISAQNIPLYLSTISTFATLVTVIYSVTK"
     gene            complement(8736..10583)
                     /locus_tag="PLZ54_05965"
     CDS             complement(8736..10583)
                     /locus_tag="PLZ54_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOJ66506.1"
                     /translation="MDSINKTDFSELITDKASQNVSAELSVGENNSAIEDQNTESTPS
                     VVSNYSTLSNEELIEALKKCLNQEVEEVKNDVESIKQAFYRNHRAEVENQKKLFLENG
                     GVEEDFVPKPNELEETFKSLLKEFRDKKAEHLARVEKEKQDNLLKKQQILEKMKILAD
                     AKDDVSTHINEFRALQQEWKSIGQVPPSSLSELFKQYNAYQEAFWDLIKINNELREYD
                     FKKNLEAKIQICEAAEKLMDEPDVVTAFQHLQKLHNEWHEIGPVSRELREEIWQRFKN
                     ASTAINKKHQTYFESIRKIEEDNLAAKTALCEKIESIDFSVLKTFSEWEEATKNVLAL
                     QDEWRTIGFAPRKLNQKVFERYRHACDAFFSAKAEFFKQVRNSYAENYRKKVELCENA
                     ESLKDNTDWKETSEKFIQLQKEWKAIGPVPRKYSDELWKRFISACDYFFDQKKKNTNS
                     LHGEELENLEKKKNLIARINSLKKSDHPHETVATLKALIEEWSTIGHVPFKEKDKVYK
                     DFRAAVDKKYEELNIDWVSRRLETFKNNLKDMNTKGQRKLIHERDKLLHARERLNSEI
                     ATYENNLGYFTSTTQKGSKVILELERKIDTLKEESRLIEEKIRLIEDNL"
     gene            complement(10755..11531)
                     /locus_tag="PLZ54_05970"
     CDS             complement(10755..11531)
                     /locus_tag="PLZ54_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ66507.1"
                     /translation="MTTSQNNKIAFVSGATSGFGRAIAFRLGKLGYRLIITGRRNERL
                     QRISQQLKEEYSTDVFSLCFDIRDKNACEQAIQSLPADFQNIDLLVNNAGLAAGASSF
                     EESDIADFEQMVDTNIKGLLYITKLIVPKMVERKSGHIINISSIAGIEVYPGGSVYCA
                     TKHAVNAITKGLRIDLVKYGIKVSSISPGMAETEFSLVRYHGDVEKAKAVYTGLIPLN
                     AEDIADAVEFIITRPKHVSINDIQINPAQQANTYISFRNT"
     gene            complement(11562..>11874)
                     /locus_tag="PLZ54_05975"
     CDS             complement(11562..>11874)
                     /locus_tag="PLZ54_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOJ66508.1"
                     /translation="KLQEQGMDTVEANLHLGFDADERDYGVGAQILKDLGVTKIRLMT
                     NNPVKRIGLEAYGLSIVENVPIEIKPNPYNAFYMQTKKERMGHTLRYVNGDKKIDTDI
                     L"
BASE COUNT         3883 a         2220 c         1939 g         3832 t
ORIGIN      
        1 tgtccacttc actcgaaaaa cttacgctcg aaaagctgaa atcgcccaaa ctcatgccga
       61 cgaaatacgt cggaactcaa acaaggacga tttataaagg cttttctcac ttttttcggc
      121 tcaccggaca aggtcaaccc gaagtgtttg gcttatatgt gctgttttac agatatataa
      181 aacaaatgtt tcaatttttg tcatgtgcta aaaaatttgt atctaaatat ttattaaact
      241 tattcgtatg aattctcttc aaattgtagg ttctcttgct tctcttttgt cgatattaaa
      301 ccaatttccg caagccataa aagtttttaa gactaaggac acccacagta tttcactaac
      361 catgtattgc attgtggtag tatgtatcac attatggctg gtttatggga tcatgttaaa
      421 agacggccca ttaatttggg caaatggact ttctcttttt cccatcactt acatttttat
      481 agttaaactt accaacacca tcaaaggaaa agacaaactc tccatttgat tattgttgtt
      541 ccgtaaaaaa cttgactaaa aaatagcatt tcattcaaaa ctatcttttg taactaaatt
      601 tgcctcacat aaatacattt ttttctaatg gaatctaaac gacaataaac ctgatgcatt
      661 aggtttttaa tttacgaatc gtaaattcat gtaattaatc taagtttttc agagggaata
      721 aacggtaaga aaaaatattt ttcaagtcaa agtaggtaaa acattccatt cctttgtcct
      781 gataaaataa atgctttact tcgggctctt cccattaaaa aactacaaag atgaaacatt
      841 taactgtaga ggaaaggtac gaaatttgaa cagtattaca acaaggattg aaacaaaaag
      901 atactctatt gactaccgga aaagaaaaga aaaaacaaaa tatcgttgcc ttccgatgat
      961 gcagttgtaa aatctctatc ttttttccct caggaaagcc actaaaaaat ggacaatccc
     1021 cattcaaaac caggacattt ctttgtcgaa agagtaaaat tttaattttt aaaccaaatt
     1081 tttaatttac acgcttcgag gaaaagtatt acaaattcgc agcgttatca actactccaa
     1141 cactgattat ttgctcgaaa agttaaacgg atatagaaaa aggatttgat tcatattgca
     1201 ggattgcaag caacaacctt tggtgttgag ttaaggaaaa aatagcataa aacataaaaa
     1261 atctgctttc aaaaaatcaa aacatatcaa aagcagattt aaaatttaac caaatggtct
     1321 tatccgtaaa atttcttatc caaattcatc caaagcagat aaaattctta tttgcttgaa
     1381 caattattgc atacaccata ataaaatatt tcaactgaat tgactttgaa attatcattc
     1441 agtggcaatt gaaaaacttc cggacgaaca tcaaaaacat cagaaacctt cccacattgc
     1501 tggcaaataa aatgagcatg cggggtagta tcgacatcat aacgcgcatt cttctcgtcg
     1561 atggtcaaca ttcgcgcagc tcccctttca atgaaaattt tgagcgtatt gtaaactgta
     1621 gttttcgaaa gtgtcggcat agaagggctt aaaaccgaaa aaatttcatc aactgtagga
     1681 tgcgttctgt tattcaacag ataatccata actgctgtcc gttgaaccga tggttttata
     1741 gaatgttgaa taagatattg ttgtgaatag gctatagaat aatacataat ggtttcttat
     1801 ttttgctggg caaagataca atatactatt gacactgaca agatttcaaa cttgaattaa
     1861 atttaaattt ttacttccca ttcggcctac tgtttaaaaa atttaaaagg ttatgagctt
     1921 gtcgcatcat aaccttaaaa agcaaaaaaa agaaaaaaaa ttatattaca cccaaatcct
     1981 tgagcatagc attggttgcg cgcaaacttt cagaactttt tatgaataat tccttttctt
     2041 cagaatttag gtgataatct aaaatttctt caaccccatt tctacctatc agcacaggaa
     2101 cgccgataca aatatcgttt tgtccatact ctccttccaa gtaaacagag cagggtataa
     2161 ttttcttttc atcatgaata atagcttcta ccagaaacgc agcagctgct ccaggcgcat
     2221 accaagccga agtacccaac aaacttgtaa tagtagcccc acccagcata gtatccgaaa
     2281 ctactttgtc caatgtttct ttatcgagca aatcggtaac cggacgacat ttataagctg
     2341 cataacgtac cagtggaatc attgaggtgt cgccatgagc tccaatgaca aaaccttcaa
     2401 tctcattttg attagcattc agagctttac ctaaataaca tttaaaacga gctgtgtcga
     2461 gtgttcctcc cattccgatt actctattgc gagggagacc tgcgatttta taagtaaggt
     2521 aagtcatggt gtccatcgga ttacttacta ttaccaaaat agcattggga gaatatttta
     2581 aaacattctc aactacactt ttaataatgc cggcattaac atctaccaat tcttcacgtg
     2641 tcatacccgg tttgcgagga actcccgaag tgatgactac tacatccgaa ttggcggttt
     2701 tttcataatc gttcgtacaa ccggatatcc tggtgttaaa atgaagcaaa gtagctgttt
     2761 gatttatatc taaagcttta ccctcagcta ttccttgttt aacatcaagc attacaacct
     2821 catcggctat ttgatagaaa gaaagaacat ttacacatgt tgcgccaaca ttcccggcgc
     2881 ctatcacact tacttttgac atactatttt aatttaaatt attaatccga gaaaagcatt
     2941 gcaaaattac aaattaaaat gattagtgaa aaatttcttt aattatttta gcataaatat
     3001 atttatctcg agaaacgaca ttgttatggc attccaaaca gaaaaattaa acaaatctca
     3061 tttgaaaata aacaaaataa acgtaatttt gtagaatgta aattatatgt gattatgaaa
     3121 cccacatgtg ttgcatacac aaacaagaat gaatatatta ttcagacatg ttggttccat
     3181 cagaccttta aaaataaatt atattctgat gaaaaattat ttttattcac ttgtcattat
     3241 tattttgctg attggttgtc aaaataaaaa ttcttttact gttgaaggga acatatcttc
     3301 agccaaagga gaaaaattat acatagaaca catggggtta ttaaaaacaa cttctgtaga
     3361 ttcagttaaa cttggtaaaa acggaaaatt tcattttacc ataaaacgac ctgaatatcc
     3421 cgatttttac cgcttgcgac tgaacgataa attaatcatt tttgccgtag actcaacaga
     3481 aaaaattaca atcaaagcaa gtaaagaaga tttgccatac aattacacac taacgggttc
     3541 tgaacaatcg cttaaaattc aacaattgag gaaatcggta gccaatattc aaaacaagat
     3601 caacaaattt tcaagcaact tagctccaga agagcaaaat cagctaatga aagaaattga
     3661 aaaagacatt gaaaatcata agcgggaagc tcaaaaatta atcttggaaa atccacgctc
     3721 tacagctgct tattatgcac tttatcagaa agtgaatgat ttttatttgt tttcacctta
     3781 cgtaaaggag gatagatctt attgcgcggc tgttgccact gcttatcaca ctttcatgcc
     3841 taactacacc agaagtcaaa acctttacgc aacggtaatg gacgctataa aaaatgaaag
     3901 aaaagcagaa agtcaactac gattggaaga gttatttaga acaaacagca ccggctacat
     3961 taatattgtt ttgcccgata agaatggaat acagcaaaaa ctgtccgatt tggaagggaa
     4021 aattgttttg atcgattttt caatttatac agctcgtgaa aacgttgaac atacttttac
     4081 gctgcgtgag ttatacaaca agtatcacga acgcggtttt gaaatttttc aagtttcact
     4141 tgatgaaaat cgctttcttt gggaaaaata cgttcagaat attccttgga tttgtgttcg
     4201 agacgaacga ggacccaata cgccatatgt actttcttac aacatttcta ctataccggc
     4261 atcttttttg atggacaggc aaggcaatat tattgcccgt gatttggatt tcaaacaatt
     4321 ggacaaacaa atagcaaaac ttttgtagtt tatttgatac aataaaaagt ttaatcgtaa
     4381 tctataccgc tcaattcggt tgtctttctc aacaaaaaat cgaatgagaa atttcattat
     4441 caatactgca ctctctatcg gattcgatgc ttgcggaata accaaagccg aaacactttc
     4501 tgcagatgca agttatttgc agtcgtggct aaaagcaggt tttcacggaa gcatgaaata
     4561 tctggaaaga aatttcgaca aaagagtgaa tccaaaatta ctggttccgg ggtgtgaatc
     4621 aataattgta gttctgatga actattttcc atccgaaatg caattgtcag ccgcgcccca
     4681 aattgcaaaa tacgcttaca gcaaaatcga ttaccacaaa gttttaaaga caaaacttca
     4741 acagttggaa aaagccatca cagaaaagtt tggtaaaaac agttttaacg atgaagtaca
     4801 acatattttt gtggactctg ctcccgtact ggaacgtcga tgggcagaaa aagccggttt
     4861 gggatggatt ggaaaaaaca aacaactcat ccatccggga ttgggttctt atgtctttat
     4921 cggaatttta atgatgaatg ccgaaacaga atatgatttg ccaattaaaa accgctgcgg
     4981 aaactgccga aaatgcatcg actcctgtcc cactcaagcg ctatcggaag aatggctgga
     5041 tgcaaataaa tgtatttctt atctgactat agaatgcaag acaaatattc ctgaagaatt
     5101 tcagggcaaa ttatccaatt acgcggtcgg ctgcgatatt tgtgccgacg tttgtccgtg
     5161 gaacaggaaa tgggcacaac ccaacaggca tcccgaattg gaaacagctt ctcccataaa
     5221 aaaatggaac tgcaagcaat gggaaaatct caaccaagag gaatttgaat cagcgttttc
     5281 gaaatctgcc atcgaatacg ttggctatag caagctgaaa gaaaacattt ccatattgat
     5341 ggaaaagttc aaaaaggact aaaaaattaa taaagtgtca gtttatcact tattcttttt
     5401 tagcttgttg agtctttttt ccaaagcatt ttgagtagtc tctctatatc tgtacttata
     5461 tttcttttca tttttatatc catatccata atgagaaaca tcatttgttc cattcaatac
     5521 agtagccatg tttttaagct ttttggattc atacgtatga ttaataaaat gtaaagcttc
     5581 ttttggcgta tatttaagac gagctacaca aatagtattg tccaccaaac gattgagttg
     5641 gaatgtatcg gaaacaattc caataggagc cgaatcgaga ataatataat cgtaacgaaa
     5701 tttcaattcc ctgatcaatt catcaagccg ttggctcatc aatagttcag cgggattggg
     5761 aggaacaggt cctgctaaaa taacatcaag atagggatgt acctcggaag gctgaataat
     5821 ttcatccatt tgacaattcg gatcagccaa aaatattgtc aaccccttat tgttcttttt
     5881 taaattaaaa tattcagcca acatgggatt tctgatatct acgcccacca gaatgacttt
     5941 ctttttcatt aaggcaaagg acatggcaag atttgttgca ataaaggtct tcccttctcc
     6001 actaatggtg gaggttacca atatgacagg tgattttatg tcggaaaaca taaactgcaa
     6061 attggaacgc actagcctga acatttcaac tatgggagtt gttcgacctt ctttcactac
     6121 aatattttcc gtttctttac ttaacccaat agttccaaga aatggtgcct tgacaaattt
     6181 ctgaaattct tttctatctt gaactgtatt attaaaaaat tccatcagat acatcaccaa
     6241 tatcggcaat ccgagtccca aaatcaacgc aagtaaaaaa ataatcattc ttttgggaga
     6301 aataggtgaa ccggtggtat aggctgcatc aattgttctg gctgatggaa tagtacttgc
     6361 cagatttaga gcattttctt ctcgtttctg aagcaaaaat acgtagaggg attccttgat
     6421 ctgctgctga cgtttaattt ccataaactg ccgctcctga gtgggaacac tcttaatttt
     6481 tgaagtaaat tcattgtttt gagccattaa atcgtttttg gaaataatca atccatcttt
     6541 tacaccggca atacttttta caatgttact gcgaatggca ttgatttgtt gctccatttg
     6601 ggtaataacg gggttttgtt cgttcgagct tctcaaaatt ttcattcgtt ccaataaggc
     6661 aacattgtat tcttcaataa gtttttgcaa ggctggatct ttaataccca agttcgcagg
     6721 aatcagattg aactggtttt tactctcgtt gatataactt tcaacataat ttaccaaatt
     6781 gagctgtgtt tctatatcgg caagtttttt ttcatattca ctagccgttt cgagataaag
     6841 ttgggcttca gatgaaatat cagttaatcc gtgagtcttt ttatagtttt ctacatttaa
     6901 ttctacatcg agcaattcgc tgctaataag ccttaaacga ctctcaataa aatcggcagt
     6961 attggtcaat atcatatttt tatcaaaaat ggcatccaaa ttataaagct caaccaattt
     7021 atcgagcatg acttttgcct tgtgaggtgt aggcgaaaca gtactaatgc tgatggcatt
     7081 agaattcttg ttcactgtag ccacagaaat ggacgctgcg agctgattgg ttaaggcagc
     7141 aagtggatag gaaataattt ttatcttttg tccacttttc aagggaccat ttacaaaaaa
     7201 tcgaattgaa ccaataggtg tagaaagtgg tgtggaaaga tcggatactc gatatttttt
     7261 ttcaataatt tctccatatt gaaatgaaat atcgtaacct tttgaagtag gagacaatat
     7321 taagattatc ttttgcttta aagtgtcatt gaaattattt gacacgaaaa tctgaaccgg
     7381 actgatggga taaacttcat catatcttaa tccatttttt acatagtatt ctgttcctag
     7441 attcaaggtg tcaattactt gcctaacaag agttttagaa gaaagtacct gaatttcatc
     7501 ttccacctct ttcgaaactc ccaacaaccc catattagaa aacatggtca tgttggtcaa
     7561 ttcaggagtc gaattttgat tacgaagcaa gatggtggtc tgaacctgat atttgttgtg
     7621 ggtaattttc agatacagga aggctacgat aaaacaaaaa acaattgaaa taacaaacca
     7681 atgccattta ctcagatatt ttattagtaa ttgccttaaa tcaatcactt cattttcgtt
     7741 tgcagcaacg cgagaatcaa tattgttttc catttataac agataaaaat aacacaattt
     7801 aaactaaaaa ttattttgta acactataaa taaccgtaac caaagtagca aacgtagaga
     7861 tggtagatag atacaacgga atattttgag ccgaaatgct tcttacaacg ttgggttcta
     7921 catataaaat atcgttttgt tgtaaataat aataaggaga ggtaaacaca tcctcggaat
     7981 tcaaatttaa acgaacaaat tccaattttc caaagttttc acgtatcaac agcacatttt
     8041 ctctctttcc gtaaactgtc atgtcgccag ccaaacccaa agcatccaac actgtaactc
     8101 tttcgttgtt gatggaatat tgtccgggac gaagtacttc acccagaacc gtaactttat
     8161 aatttaaaaa ttgtatggtg ataatggcat ttttcaaata aggactcaat aactcactaa
     8221 tataagcaat tgcttccgat ttggtcaatc caaccaattt tattggtcca ataatcggaa
     8281 aattgatata gccgttaata tctaccaaat aagattgcaa aacaggagaa gcgtaaattt
     8341 gtgtagatcc aggattggca taagaaacca aaggcaagtt gaaaggagca acagcttcag
     8401 ggtctaagcc cgaaactgtt attgacaaca tatcacccgt acaaatacgc gcttcatgcc
     8461 ctttggtgaa agattgatta atatttgcga ccgatttttc attaacacca taaaaataag
     8521 caattttttt ctgagaggta catcctgtga ataaaacaat taacagagta aaaaaaagga
     8581 aagaaaaatt tctcataatc agttattttt cagtattcat actcaaaata aaaaatttac
     8641 aaccgttagg tttataacac catttatcaa aaaaattaac gactttaaaa tgaaattatt
     8701 ttatcatttt aaagttaaaa aaatttcctt gcgccctata aattgtcttc aatcagcctg
     8761 attttttctt ctattaagcg gctttcttct ttcagggtat caatttttcg ttccaattcg
     8821 agaatcactt tactgccttt ttgggtagta gaagtaaaat aacccaaatt gttttcatag
     8881 gttgctattt cagaattaag tctttcccgt gcatgaagca atttatccct ttcgtgaatg
     8941 agttttctct gccctttggt attcatgtct ttcaaattat ttttgaaagt ctccaagcga
     9001 cgacttaccc aatcgatatt gagctcttca tattttttat ctacagctgc acgaaaatcc
     9061 ttgtaaacct tatccttttc tttgaaaggt acatgaccaa tggttgacca ttcttctata
     9121 agagctttta aagtggcaac cgtttcatgg ggatgatccg atttttttaa tgaattgatt
     9181 ctggctatca gatttttctt tttctcgaga ttctccaatt cttctccatg tagagaattc
     9241 gtgtttttct ttttttgatc gaaaaaataa tcgcaagcag atataaaacg tttccaaagt
     9301 tcatcggaat attttcttgg caccggacca atcgccttcc attccttttg taattggata
     9361 aatttttcgg aagtttcttt ccagtcggta ttatctttta aagactcggc attttcacaa
     9421 agctcaactt ttttgcgata attttcggca taactgtttc tcacctgttt gaaaaattct
     9481 gctttagccg aaaaaaaggc atcgcaagca tgccggtaac gttcaaaaac tttttgattg
     9541 agttttcgcg gtgcaaaacc aatggttctc cattcatcct gcaaggccag tacattttta
     9601 gtagcttctt cccattcact gaaagttttt aatacagaaa aatcaatgga ttctattttt
     9661 tcgcaaaggg cagttttggc agccaaatta tcttcttcta tctttcgaat ggattcaaag
     9721 taagtctgat gttttttatt gatagcagtg gaagcatttt taaaacgttg ccagatttct
     9781 tctcgtaact cacgtgaaac aggaccgatt tcatgccatt cattatgaag cttttgtaaa
     9841 tgttgaaatg ctgtaactac atcgggctcg tccatcaatt tttcggccgc ttcacaaatc
     9901 tgtattttag cttccagatt tttcttgaaa tcatattctc tcagctcgtt gttgatttta
     9961 attaaatccc aaaaagcttc ctgataagca ttgtattgtt taaataattc cgacaaactg
    10021 gagggaggaa cctgtccaat agatttccat tcctgctgca atgctctgaa ttcattaata
    10081 tgggtcgaca catcgtcttt ggcatcggca aggattttca ttttttccag aatctgctgc
    10141 tttttcaaca ggttgtcctg tttttctttc tcaacccttg ccaaatgttc agctttctta
    10201 tcgcgaaatt cctttaacaa agatttaaaa gtttcttcca actcatttgg ttttggaaca
    10261 aaatcttctt ctacccctcc attttcgaga aaaagttttt tctgattttc aacttccgca
    10321 cgatgatttc gataaaatgc ctgtttaata ctttccacat catttttaac ctcttcaact
    10381 tcctggttta gacacttttt caaagcctca atcaattctt catttgaaag agttgaataa
    10441 ttcgaaacaa cagaaggtgt cgattcggta ttttgatctt ctatggctga attattttcg
    10501 ccaacagaca actcagcaga aacattctgc gatgctttgt cggtaatcaa ttcagaaaag
    10561 tcggttttgt tgattgaatc catttaaagt aacgttattc atgatttgtt ctcccatttt
    10621 ttagcgagaa cgatgctatt tcttttcaaa aacattttta tattcacaaa agtataataa
    10681 ttattttaaa tatcgatgat gtacattaaa agattttaag ctttctttgt cgaatcggtt
    10741 tattattgat tattttacgt atttctaaat gatatatagg tatttgcctg ctgtgcagga
    10801 tttatttgaa tgtcattaat ggaaacatgc tttgggcgag taataataaa ttccacagca
    10861 tcggctatat cttcggcatt taagggaata aggccagtat acacagcctt agctttttca
    10921 acatcgccgt gatagcgcac cagcgaaaat tcggtttcgg ccataccggg agaaatggaa
    10981 cttactttta ttccatattt taccaaatca attcgcaaac ctttagtaat ggcattcacg
    11041 gcatgtttgg ttgcacaata tacacttccg ccgggatata cttctattcc cgcaattgaa
    11101 gaaatattaa taatatggcc acttttccgc tccaccattt tcggcactat tagtttggta
    11161 atatacaaca aacctttaat gttggtatct accatttgtt caaagtcggc tatatcgctc
    11221 tcttcaaaag aagaagcccc tgccgccaaa ccggcattgt taaccaacaa atcgatgttt
    11281 tgaaaatctg caggcaacga ttgaatagct tgttcacaag catttttgtc gcgaatatcg
    11341 aaacagagag aaaaaacatc ggtagaatat tcttctttta attgttggga aatccgctgc
    11401 aatctttcgt ttcgtcgtcc ggtaattatc aatcgataac ccaattttcc caatcgaaaa
    11461 gctattgcac gtccaaaacc cgacgtggca cctgagacaa aggctatttt attgttttga
    11521 gaagttgtca taaaacaatt tagtaaaaaa tagaagtatt tttaaagaat atcggtatcg
    11581 atttttttat ctccatttac ataacgcagc gtatgtccca ttctttcctt ttttgtttgc
    11641 atataaaaag cattgtaggg attcggttta atttctatcg gcacattttc gacaatagac
    11701 aatccgtagg cttcgagtcc aatgcgttta acaggattgt tggtcatcaa tctgattttg
    11761 gtaacaccca aatccttcaa gatttgcgca ccaacgccgt aatcgcgttc atcggcatca
    11821 aagccgagat gcaaattggc ttcaaccgta tccatgccct gctcctgcag cttg
//
LOCUS       DAOSCH010000064        11524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3749_length_11524_cov_5.309617, whole genome shotgun sequence.
ACCESSION   DAOSCH010000064 DAOSCH010000000
VERSION     DAOSCH010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3749_length_11524_cov_5.309617"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /gene="hisH"
                     /locus_tag="PLZ54_05980"
     CDS             <1..568
                     /gene="hisH"
                     /locus_tag="PLZ54_05980"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007766333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOJ66509.1"
                     /translation="NAGNIRSVLFALERLGIEAVVTDDAEEILSADKVIFPGVGEAST
                     AMNYLKQNRLDLLIPSLKQPVLGICLGMQLMCSFSEENDTTCLGIFDDKVKHFPDNEN
                     FKVPQMGWNNIFRLSGPLFNAIKENSFVYFVHSYYVPLSSNTAAVANYIFDFSAALQK
                     NNFFAVQFHPEKSGEIGQKMLENFLNIC"
     gene            595..1725
                     /gene="tgt"
                     /locus_tag="PLZ54_05985"
     CDS             595..1725
                     /gene="tgt"
                     /locus_tag="PLZ54_05985"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOJ66510.1"
                     /translation="MNFEILYTDSGSKARAGKITTDHGAIETPIFMPVGTAGSVKTVH
                     FRELEEDIHAQIILGNTYHLYLRPGTDILEQAGGLHKFIGWDRPILTDSGGFQVFSLT
                     GIRKLEEEGVLFQSHIDGSKHFFTPEITVDIQRTIGADIIMALDECTPGTADYQYAKK
                     SMQLTHRWLLRGIEHFDTVSPKYGYSQAFFPIVQGCVYPDLRRESAKFIADQNKDGNA
                     IGGLAVGEPTEKMYEMIEIVNDILPENKPRYLMGVGTPQNILESIERGVDMFDCVMPT
                     RNGRNGMLFTSQGVINIKNAKWKNDFSPLDEKGTSFVDKAYSKAYVRHLFAANELLGL
                     QIASIHNLAFYLWLMNEARLHIRKGDFSLWKKGMIEQLGRRL"
     gene            1753..2874
                     /locus_tag="PLZ54_05990"
     CDS             1753..2874
                     /locus_tag="PLZ54_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOJ66511.1"
                     /translation="MKALKTKLKINYQSFGLKRIDTYIINKFLGTYFFCIVLILSISV
                     VFDLTEKLDNFFETNAPLKAIIFDYYLNFIPYYMNMFSPLFTFIAVIFFTSKMANNTE
                     IIAILSSGVSFRRLMLPYFISATLIALLFFFLGGYVIPNSSKKMLNFENQYVKKFKPS
                     NARNVQMEVEKGVIMYIERYEVDNNIGYHFSLEKFDGKKLVSRLTAETITWDSAYNWK
                     INNYLQRDFIGMREKITKGISMDTVIKVQPKELFITSEEAAQMTNSELRQYLHRQRAR
                     GVGNIQSFENEYYRRYAMPFAAFIMTLIGVSLSSRKVRGGMGLNLGIGLALSSIYILF
                     STLSTTFSVNGTLSPFMAVWLPNIVFLLIGIYLYQTAPK"
     gene            complement(2931..3995)
                     /gene="rfbB"
                     /locus_tag="PLZ54_05995"
     CDS             complement(2931..3995)
                     /gene="rfbB"
                     /locus_tag="PLZ54_05995"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOJ66512.1"
                     /translation="MEKTILITGGAGFIGSHVVRLFVNKYPDYHIVNLDALTYAGNLE
                     NLKDVADAPNYTFVKGDITDEEFIPQLFEKYDFDGVIHLAAESHVDRSISDPLAFIRT
                     NVFGTANLLNAAKEAWKGKMEGKRFYHISTDEVYGSLGKEGYFTEKTPYDPRSPYSAS
                     KASADHFVRAYYHTYGLPVLISNCSNNYGSHHFPEKLIPLCINNIRYNRPLPVYGKGE
                     NVRDWLWVDDHAAAIDIIFHQGEIGETYNIGGHNEWTNIRLIEKLCEIMDKKLGRESG
                     ESAKLITFVKDRAGHDLRYAIDSSKLQRELGWQPSITFEEGLEKTVDWYLENQEWLDN
                     IINGDYQNYYEKQYSEREKS"
     gene            4114..5064
                     /locus_tag="PLZ54_06000"
     CDS             4114..5064
                     /locus_tag="PLZ54_06000"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66513.1"
                     /translation="MEKLSRYIEILLAQHDYVIVPGLGGFIIQPQSAMILSDRITPPL
                     AVIAFNPLLNSNDGLLAYEISEREHISYRAAIEMIDHEVESILSKLRQGEKVDMENLG
                     SLQKNANGHLIFSPVPRADFLPANFGMTEIKLWKRRITDSEERKKITITLPSASSFRY
                     AAACALIFGMLLVSPRMADTKRSHYANLIPLETYTPTQSQPSASKNVSINNTLKATVE
                     TEKQDSLSPAKEENFQNALSKQTNLYHVIAATFYSRKSAEKFCAQLQNLKYTEARVFS
                     PEKNYKVVVRSFPRYQEAVEFMEQLRKSDALFESAWVYCK"
     gene            5172..5705
                     /locus_tag="PLZ54_06005"
     CDS             5172..5705
                     /locus_tag="PLZ54_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005642538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOJ66514.1"
                     /translation="MPVKIRLQRHGHKGYAFYHIVIADSRTPRDGRFIERIGSYNPNT
                     DPSSVDLKFDRALYWLQVGAQPTDTVRNILSREGVLMKKHLLEGVKKGAFDEAEAERR
                     FEAWKSSKEAKLNKTKEQLTAEREATEKARLSAEIEANKARAAELAKKKAAEEAKTKE
                     KDNSSENSEEQEPKEVE"
     gene            5897..6190
                     /locus_tag="PLZ54_06010"
     CDS             5897..6190
                     /locus_tag="PLZ54_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HOJ66515.1"
                     /translation="MNKIVNIALQILPSSATIHPYKLVDKAIEVIAASGLKYRVTPFE
                     TVMEGTYDEVMEVVKKAQQACYDAGAESMMTYIKIQSSKDTDVRIEDKMEKYE"
     gene            6254..7459
                     /locus_tag="PLZ54_06015"
     CDS             6254..7459
                     /locus_tag="PLZ54_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polymerase"
                     /protein_id="HOJ66516.1"
                     /translation="MIYATLITLLSLLISLRISKNIFAPATIVSGLWLFCILAFNLYP
                     HDLFPLKGQFYTGISIWVGVFTLSCLLTQSIFQKPNNIDDPNQSLVNLYFLITIITFP
                     YVLWNIYNLLKNAGLLNNVFYNLRSAALGNIKGLEEGTAKNYFAPLWIVTYLIELQHF
                     KKERIWIFISLLLINLSWVFLIMSKLSFLIICVSTLMILFFKKIIKTKIILISLAVLF
                     IFFSYFQMLRSTQSEINNKELNYDMFTLYVLSGMPAFETVKPNSSEFFGQNSFRFFYA
                     IGYKTGLTNKKPARPVLEFINIDKNNKTHTNVYTTLYPFFKDFGYKGIFIFAFLIGIF
                     YGYIFKKATKKDNPMVITYSILVVSLVTQFMNEATLTTLSFLLQILIFAHLPYWVNKK
                     ITIQNTVEN"
     gene            7476..8750
                     /locus_tag="PLZ54_06020"
     CDS             7476..8750
                     /locus_tag="PLZ54_06020"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HOJ66517.1"
                     /translation="MTENKQEIKDVIYLMALQGLNYAAPLIVFPYLMKVLGAEKFGYI
                     GFSLSINQYLMLLVDFGFNFSATKKIALAKNNPDELNRIFSATLWAKISLLFISFLIL
                     LIFAFFIPKFEIYSTTMIILFIMVVANTFSFVWLFQGLGQIRVISIVNSISKLLILPF
                     TFILVKSPEDYNVAAFIQSSVYVFGSLLTILILIKRKYISSWLSIQKLKINEEIKSAF
                     PIFLSSAASSVYTALFAVILGYYSTPIEVGKYTAAEKIMRGFCFLIFTPISQAFYPKV
                     SLLGVSSLQEAQKLTKKILKNITLIMMVLFFLLFFFSSPITHFLGKDYSGTDTIFKIM
                     SIAPLFIASGGILGQFGLLAIGNEKDKRNFQNVYLTGAIVALITVFVLVPHFNSIGAS
                     ISLLITEISVFTGMFWFNKKLFLTKTFDENYI"
     gene            8750..9577
                     /locus_tag="PLZ54_06025"
     CDS             8750..9577
                     /locus_tag="PLZ54_06025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ66518.1"
                     /translation="MENSIEIVLVLYHCSLDDSATFSTLGEPLKRTSLNCELILFNND
                     QSQKIESTEYYVVNSNENVKLAGAYNFALERARVKKKNWILLLDQDTVVPENYFTELE
                     KLFSEGISKDLAAIVPRLEYNDRQISPVKVSRWMRMEQEMTTIGYTSCRMNALNSLSL
                     FRVDFLSSIGGFNKDYPFDMLDHWSYNQIHKHGKLVYIMDTVGKHNSSFVDFETNVSV
                     SRYKEFMDAQKTFIRKELGIWNYLFYKVKLLIRGFSQLLKYKNKRFAEITFASIFKR"
     gene            9621..10556
                     /locus_tag="PLZ54_06030"
     CDS             9621..10556
                     /locus_tag="PLZ54_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004369945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOJ66519.1"
                     /translation="MRILVTGGAGFIGSHLCDALIQANHEVIVVDNLILGKMENINHL
                     MKLPNFHFYKEDILNFEGLKQIFNKHAFDMVFHMAANSDIQKGSADPEIDFNLTFKTT
                     FNVLQCLKEFNVKKLFFASTSAIYGETSDLLTEDYGPLRPVSNYGAGKLASEAFISAF
                     SAAYDIQTWITRFPNVVGERFTHGVIYDFIHKLKSNPNELEVLGNGEQNKPYLYVKDL
                     IEGILFVCNNSHERFNVYNLGSNTRTKVKEIAKMVIEEMGLNARINYTGGDRGWVGDV
                     PEFKYDVSKINQLGWKAKYTSNESVRLAIQKALGK"
     gene            10553..>11524
                     /locus_tag="PLZ54_06035"
     CDS             10553..>11524
                     /locus_tag="PLZ54_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOJ66520.1"
                     /translation="MKVVIIAGGKGTRISSITNEIPKSMIKVEERPVIEYQVELAKRY
                     GFNEFIFIIGHLGEQIETYFGDGSKWGVSIQYFKEKEALGTAGALTFLTEELKEDFFV
                     FYGDTVMDVDLLRMWNFHQKKQSDATLFVHPNDHPYDSDLVDIDEEGKIKNFFNKPHS
                     PEFIGRNLVNAALFIFSPKILQNIQKGVKLHIEKDVLPKCLNQGMNLYGYVSPEYVKD
                     MGTPDRYMEVCEDVRSGKVARMNWNNPKFAVFLDRDGVISKEIDLLSSMDQLELIDGA
                     GEAIRYINQKGYLAIVVTNQPVIARNLCSLDELAEIHAKLETLLGEKQ"
BASE COUNT         3745 a         1984 c         2145 g         3650 t
ORIGIN      
        1 caatgcaggg aacatccgat cggtactttt tgctttggaa cgactgggaa ttgaagcggt
       61 tgtaactgat gacgcagaag aaatcctatc tgccgacaag gtaatttttc ccggtgtagg
      121 tgaagcttct acagccatga actatctgaa gcaaaaccga ctcgacctgc ttattccttc
      181 actcaaacaa ccggtattgg gaatctgtct gggcatgcaa ctcatgtgct ccttctctga
      241 agaaaacgat acaacttgtc tgggtatttt tgatgacaaa gtaaaacatt ttcctgacaa
      301 cgaaaatttt aaagttccac aaatgggctg gaataatatt tttcggttat ccggaccatt
      361 gtttaatgcg ataaaggaaa attcgtttgt ttattttgtc cacagttact acgtaccact
      421 gtcgtcaaat accgctgctg ttgccaatta tattttcgac ttcagtgcag ccttacaaaa
      481 gaacaatttt tttgccgttc agttccaccc ggaaaaatcg ggagaaattg gtcagaaaat
      541 gctggaaaat tttttaaaca tttgctgaac ggaatttttt gatagaaaca ccgaatgaat
      601 tttgaaatac tttatactga ttccgggagc aaggcaagag cagggaaaat aaccactgat
      661 cacggcgcaa tagaaacacc tatttttatg ccggtaggaa ccgcaggttc cgtaaaaact
      721 gttcattttc gcgagctgga agaagacatt catgcgcaaa tcattttagg aaatacttat
      781 catttgtatt tgcgacccgg cactgacatt cttgaacaag ccggcggact gcacaaattt
      841 attggctggg atcgccccat attgaccgac agtggaggct ttcaagtttt ttcgcttacc
      901 ggtatccgaa aattggaaga agaaggcgtt ttgtttcagt cgcacatcga tgggagcaaa
      961 cattttttca ctcccgaaat tacagtggac attcagcgaa ccattggagc agatatcatt
     1021 atggctctgg atgagtgtac accgggcaca gccgattatc agtatgcaaa aaaatcgatg
     1081 caacttactc atcgatggtt gctcagagga attgaacatt tcgatacggt ttcacccaaa
     1141 tacggatatt cccaagcatt tttccctatt gtgcaaggat gtgtctatcc cgatttgaga
     1201 agagaatcgg caaaatttat tgccgaccag aataaagacg gaaacgccat cggcggactg
     1261 gcagtgggcg aacctaccga aaaaatgtat gaaatgattg agattgtaaa cgatattctt
     1321 cccgaaaaca aacctcgata cctgatgggc gtgggaacgc cacaaaacat actggaaagc
     1381 attgaacgcg gagtggatat gttcgattgc gtcatgccaa cgcgcaatgg tcgaaatgga
     1441 atgcttttta cctcgcaagg cgttatcaac ataaaaaacg ccaaatggaa aaatgacttt
     1501 tcgccgctgg atgaaaaggg aacttcattc gtcgataaag cttattccaa agcttatgtg
     1561 cgacatttgt ttgctgccaa cgaattattg ggactgcaaa ttgcttctat tcataatctg
     1621 gctttttatc tttggcttat gaacgaagcc cgactgcata tccgaaaggg agatttttca
     1681 ctatggaaaa agggaatgat tgaacaactc ggtagaaggc tttgaaaata cctttattca
     1741 aaattttctg gcatgaaagc tttaaaaacc aaattgaaaa taaattatca aagtttcgga
     1801 cttaaaagaa tcgatacgta catcatcaac aagttcctgg gaacttactt tttctgtatc
     1861 gtactgattt tgagcatttc tgttgttttc gacctgactg aaaagttgga taattttttt
     1921 gaaaccaatg cgcccctcaa agccatcatt ttcgattatt atttgaattt tattccatat
     1981 tacatgaata tgttcagccc cctgttcacg ttcatagccg taattttttt cacctcaaaa
     2041 atggccaaca atactgaaat tattgccatt ctttccagtg gtgtgagttt tagaagatta
     2101 atgcttccct attttatatc tgcaacgctc attgctcttt tgtttttctt tctgggtgga
     2161 tatgtcattc caaattccag caaaaaaatg ttgaattttg agaatcagta tgtcaaaaaa
     2221 tttaagccca gcaatgcccg aaacgtgcaa atggaagttg aaaaaggtgt aatcatgtac
     2281 atcgaaagat acgaagtcga caacaacatt gggtaccatt tttcactgga aaaatttgat
     2341 gggaaaaaac tggtttcgcg tctgacggca gaaacaatta catgggattc ggcatacaac
     2401 tggaaaatta ataattattt gcaacgcgat tttatcggca tgcgtgaaaa aattacgaaa
     2461 ggaatttcaa tggataccgt tatcaaagtt caacccaaag aattatttat tacttcggaa
     2521 gaagctgctc aaatgaccaa ttctgaactc agacaatatc tgcaccgcca gcgtgcgcgt
     2581 ggtgtgggca atattcaatc tttcgaaaat gaatactatc gccgttatgc catgccgttt
     2641 gctgctttta tcatgacttt aataggagtt tcgctttcgt cgcgaaaagt acgcggaggt
     2701 atgggactca atttgggaat cggattggct ctcagttcga tatatatttt gttttccacg
     2761 ctgtcaacca ctttttcagt caatgggacg ctttcacctt tcatggctgt atggctgccc
     2821 aatatcgtat tcttgctcat cggcatctat ctttatcaga cagcaccaaa ataagagtag
     2881 aagaaatttt tgtgaaaggc gaaattgcaa aaaatcaatt tattccatac tcaacttttc
     2941 tctctttcgg aatattgttt ttcgtaatag ttttgataat caccgttaat aatattgtcg
     3001 agccattcct gattttccaa ataccaatcc actgtttttt cgagcccttc ttcgaaagta
     3061 atggagggct gccagcctaa ttcgcgctgt aattttgacg agtcgatggc ataacgcaaa
     3121 tcgtgtcctg ctctgtcttt gacaaaggtt atcagtttgg ctgattcacc ggattcacgt
     3181 cccaattttt tgtccataat ctcgcacaat ttctctatca aacgaatgtt tgtccattcg
     3241 ttgtgcccgc ctatgttgta agtttcgcct atttcccctt gatgaaaaat gatgtcgatg
     3301 gctgcagcgt ggtcatcaac ccacaaccag tctctcacat tttctccctt tccgtaaacg
     3361 ggaagtggcc gattgtagcg aatattattg atgcaaagcg gaatcagttt ttcggggaaa
     3421 tgatgcgaac cataattgtt ggaacaattg gaaatcaata ccggcaaacc atacgtgtgg
     3481 taataagccc tgacaaaatg gtcggcactt gctttggaag cggaataagg gcttcgggga
     3541 tcataaggcg ttttttcggt gaaatatcct tcttttccca acgaaccata aacttcatcg
     3601 gtagaaatat gataaaatct ttttccttcc attttgccct tccatgcttc tttcgctgca
     3661 ttcaaaaggt tggctgtgcc aaatacgtta gttcggataa aagccagcgg atcactgata
     3721 gatcggtcga catgcgattc ggcagccaaa tgaataacac cgtcaaaatc atatttctca
     3781 aataattgag gaataaattc ttcatccgtg atgtctcctt ttacaaaagt ataattggga
     3841 gcatcagcca catccttgag attttcaaga tttcccgcat aagttaacgc atctaaattt
     3901 acaatgtggt aatcgggata tttatttaca aaaaggcgca ctacgtgaga accaataaaa
     3961 cctgcaccgc cggtaatcag tatagttttt tccatcgtta ataactgaat tgaatcatac
     4021 aaagataatt cgatttttca catttaacaa aatgttgaaa acttttttgt gtaaaaatac
     4081 ctttttaaaa attctttcta ttatatttgc aacatggaaa aattaagtcg gtacatagaa
     4141 attttgctgg ctcagcacga ttacgttatc gttccggggt tgggaggatt tatcatacaa
     4201 ccacaatcgg ccatgatttt atccgacagg ataacgcctc cgcttgccgt aattgccttt
     4261 aatccgttgt taaattccaa cgatggccta ctggcttatg aaatttcgga gagagaacat
     4321 atcagctatc gagccgccat cgaaatgata gaccacgaag tagaatcaat tttatcgaaa
     4381 cttcggcaag gagaaaaggt tgacatggaa aatctcggca gcctgcaaaa aaacgcaaac
     4441 ggacatttga ttttttcacc tgtgccgcgc gccgattttc tccctgccaa ttttggcatg
     4501 actgagatca agctgtggaa acgacgtatt accgattcag aagaacgcaa aaaaattacc
     4561 attactttgc catcggcttc ttcgtttcga tatgctgctg cttgcgctct aatttttggc
     4621 atgttgctgg tttctccgcg tatggccgat accaagcgca gtcattatgc caatttgatt
     4681 ccgctggaaa catacacacc gactcaatcc cagccttcag cttcaaaaaa tgtttcgata
     4741 aataataccc taaaagctac tgttgagacg gagaagcaag atagtctttc tcctgcaaaa
     4801 gaagaaaact ttcaaaacgc tctcagcaag caaaccaact tgtatcacgt tattgcagct
     4861 acattttatt cccgcaagtc ggctgaaaaa ttctgtgccc aacttcaaaa tttgaaatat
     4921 actgaagcgc gggtattttc accggaaaaa aattacaaag tggttgtccg ttcatttcct
     4981 cgttatcagg aagcagtgga atttatggaa cagctccgta aatccgatgc tttgtttgaa
     5041 tctgcatggg tatattgtaa ataattgatt ttttatcgat aaaaatattt cggttatcaa
     5101 ataaaaatga taactttgtg ccgcttttcg agaaaaagca attaggtatt aacaataaaa
     5161 aaattttaaa aatgcccgta aaaatcagat tacaacgaca tggacacaaa ggatatgctt
     5221 tttatcatat cgttattgcc gacagtcgta cgccacgaga tggcagattt atcgaaagaa
     5281 ttggttccta taatcccaac accgatccaa gttcagtaga cttaaaattt gacagagctt
     5341 tgtattggct tcaggtaggt gcacaaccca ccgacacggt acgcaacatt ttatcaagag
     5401 aaggcgtatt aatgaaaaaa cacttgctgg aaggtgtaaa aaaaggtgct ttcgacgaag
     5461 cagaagccga acgccgcttt gaagcatgga aatcttcaaa agaagctaaa ttgaacaaaa
     5521 ccaaagaaca gcttactgct gaaagagaag ctaccgaaaa agctcgtctg tcggcagaaa
     5581 tagaagcaaa taaagccaga gctgccgaac tggcaaagaa aaaagccgct gaagaagcaa
     5641 aaacaaaaga gaaagacaat tcttcagaaa actctgagga acaagaacca aaagaagtag
     5701 aataaatcct cttaaaaaca ttcaaatttg acttgctcta aaaaaagccc ggaagtattt
     5761 ttgggctttt ttgttttatc acggagaaga attttctgat gttattttta gaacataaat
     5821 tgcaaaaagc tacaattttt aaactgttta tacgtttcaa tctataagta gttgtcaata
     5881 tttaatctgc gtaaaaatga ataaaattgt caatattgca cttcaaattt tacccagttc
     5941 agctaccatt catccatata aactggtaga taaggcaata gaagtgattg ctgcttcagg
     6001 actgaaatac agggtaactc cttttgaaac ggtgatggaa ggaacttatg acgaagtgat
     6061 ggaggtggtg aaaaaagccc aacaagcctg ctatgatgcg ggtgcagaaa gtatgatgac
     6121 ctacatcaaa atccaaagct ccaaagacac ggacgtaaga attgaagata agatggagaa
     6181 atatgaatga tagaaaatat ttatcctaaa taaaataaaa ctttgaaaag acaataaaca
     6241 tatcaatcaa tttatgatat atgctactct cattacatta ttatctttac taattagttt
     6301 acgtatttct aaaaatattt ttgcacctgc aactattgtt tcaggattat ggttgttttg
     6361 tattcttgct tttaatctct atccacatga tttatttcct ttaaaaggtc aattttatac
     6421 aggaatttca atatgggtag gagtatttac tttatcctgt ttattaactc aaagtatatt
     6481 ccaaaaacca aacaacatcg atgatccaaa tcaaagctta gtaaatctat attttttaat
     6541 tacaatcatt actttccctt atgtattatg gaatatttat aatttattaa aaaacgcagg
     6601 gttactcaat aatgtttttt ataatttaag aagtgctgct ttaggtaata ttaagggatt
     6661 agaagaagga actgctaaaa attattttgc acctttgtgg attgtaacat atttaattga
     6721 actacaacat tttaaaaaag aaagaatctg gatttttata tctttgctct tgattaatct
     6781 ctcatgggtg tttttaataa tgtctaaact atcttttctt attatttgtg tttcaactct
     6841 tatgatatta ttttttaaaa aaattattaa gacaaaaatt attctaattt cattagctgt
     6901 attatttatt tttttttcct attttcaaat gctacgttcc actcaatcag aaattaataa
     6961 taaagaattg aattatgata tgtttaccct atacgttcta agtggtatgc ctgcttttga
     7021 aactgttaaa ccgaattcct cagaattctt tggacaaaat tcgtttagat ttttctatgc
     7081 cattggttat aaaacaggac ttacaaataa aaaaccagca cgtcctgttt tagaatttat
     7141 taacatagat aaaaataaca aaacccatac aaatgtttat accaccttat atccattttt
     7201 caaagacttt ggatataaag gtatttttat ttttgctttt ttgattggta ttttttatgg
     7261 gtatattttt aaaaaagcta caaaaaaaga caaccctatg gtcattacat attccatact
     7321 ggtcgtttca ttagttacac agtttatgaa tgaagcaacg ttaacaactc tgtcttttct
     7381 tcttcaaatt ttaatatttg ctcatctacc ctattgggta aataaaaaaa taactattca
     7441 aaatactgtt gaaaattaaa ttttagaatt ttaatgtgac agagaataag caagaaataa
     7501 aagatgtaat ttatttaatg gccctgcaag gattaaatta tgcagcacca ttgattgtgt
     7561 ttccctattt aatgaaagtt cttggagccg aaaaatttgg atacatagga ttttctttat
     7621 ccattaatca gtatttgatg ctgttagttg acttcggatt taattttagt gcgacaaaaa
     7681 aaatagcgtt ggcaaaaaat aatccggatg aattgaatcg gatattttct gctacgttat
     7741 gggcgaaaat aagtttatta tttatcagtt tccttatact attaattttt gccttcttta
     7801 ttcctaaatt tgaaatttat tcaacaacaa tgattattct gtttatcatg gttgtagcaa
     7861 atacgttttc gtttgtatgg ctttttcagg gattgggaca aattagagtc atttctattg
     7921 tgaattccat ttcaaaattg ctgattctcc cattcacatt tatattagta aaatctccgg
     7981 aagattataa tgtcgctgca tttatccaat cgtctgtata cgtattcggg agtttattga
     8041 ctattttgat tttaataaaa agaaaataca tttcaagttg gctttccatt caaaaactaa
     8101 aaattaacga agagataaaa tcggctttcc ccatattctt gtcaagtgcg gcttcgagtg
     8161 tttacactgc actatttgct gtaatattag gatattattc cacgccaata gaagttggaa
     8221 aatatacagc agccgaaaaa ataatgagag gcttttgttt tttaattttc actcctattt
     8281 ctcaagcttt ttatcctaaa gtcagtttat tgggggtgtc atcacttcaa gaggcacaaa
     8341 agttgactaa aaaaattctg aagaatatta ctctcatcat gatggtctta tttttccttt
     8401 tatttttctt ttcttcgcca atcacacatt ttttagggaa agattactct ggaaccgata
     8461 ccatattcaa aataatgtcg attgctcctt tatttattgc atcaggtgga attttaggac
     8521 aatttggact acttgccatt ggtaatgaaa aagataaacg aaatttccaa aatgtttatt
     8581 taactggtgc tattgttgct ttgataactg ttttcgtcct ggttcctcat tttaattcaa
     8641 taggtgcttc aatatcactc ttaataactg aaatttctgt ctttacggga atgttttggt
     8701 tcaataaaaa actttttttg acgaaaacat ttgatgaaaa ttatatttaa tggaaaactc
     8761 tattgaaata gtcttagttt tgtatcattg ttcgttagat gatagtgcaa ctttttcaac
     8821 acttggtgag ccattgaaga ggacttcatt aaattgcgag ttgatcttat ttaataatga
     8881 ccaatcgcag aaaattgaaa gtacggaata ttatgttgtc aattcgaatg aaaacgtgaa
     8941 acttgccgga gcatataatt ttgcattgga aagagcaaga gtaaagaaaa agaattggat
     9001 attgttactg gatcaggata cagtagttcc tgaaaattat tttacagaac ttgaaaaatt
     9061 attttcggaa ggcatttcaa aagatttggc tgctatagtt cctcgattag aatacaacga
     9121 cagacaaatt tcgccagtaa aagtatctcg atggatgaga atggaacagg aaatgactac
     9181 tataggttat acgagttgtc ggatgaacgc actcaactca ttatcattgt tccgagtcga
     9241 ttttttaagt tccataggtg gatttaataa ggattatcct tttgatatgc ttgatcattg
     9301 gtcttataac caaattcata agcacggaaa attggtttac atcatggata ctgtgggaaa
     9361 acataattct tctttcgtag attttgaaac aaatgtatcg gtttcacgtt acaaagaatt
     9421 tatggatgcc caaaaaacat ttatccgtaa agaattagga atttggaact atttgtttta
     9481 taaagttaaa ttgttgatta gaggattttc tcaattgttg aaatataaaa acaaaagatt
     9541 tgcagaaatt acttttgcgt ccatattcaa aagatgaagt gagtaaaaac tgatattgaa
     9601 agaataaaaa ttataatttg atgagaatac ttgttaccgg tggagcagga tttataggat
     9661 ctcatttgtg tgatgctttg atacaggcca atcacgaagt gatagtagtg gacaacttga
     9721 ttctggggaa aatggaaaat ataaaccacc tgatgaaatt acctaatttt catttttaca
     9781 aggaagatat attgaatttt gaaggtctga agcaaatttt caataaacat gcttttgaca
     9841 tggtttttca catggcagcc aattcggata ttcaaaaggg aagtgcagac cctgaaattg
     9901 attttaatct tacatttaaa accactttca acgttttgca gtgtttgaaa gaattcaatg
     9961 tgaaaaaatt gttttttgct tctacttctg ccatttatgg agaaacttcc gatttgttga
    10021 ctgaagatta tggtcctttg cggccggtct cgaattatgg agcagggaaa ttggccagcg
    10081 aagcatttat cagcgcattc agtgccgctt acgacattca aacgtggata acaagatttc
    10141 caaatgtagt gggagaacga ttcacacacg gcgtgattta tgattttatc cacaaattga
    10201 aatccaatcc gaatgaactg gaggttttag gtaacgggga acaaaacaag ccttatttgt
    10261 atgtaaaaga tttgatagaa ggaattctgt ttgtatgcaa taattcacac gaacgtttta
    10321 atgtatacaa tttaggttcg aacacacgta ccaaagtaaa ggaaatagcc aaaatggtga
    10381 ttgaagaaat gggattgaat gccagaatta actataccgg aggcgatcga ggttgggttg
    10441 gagatgtgcc agaattcaaa tacgatgtga gtaaaatcaa tcaattaggg tggaaagcaa
    10501 aatacacatc caatgaatcg gttagacttg ccattcaaaa agcgctcgga aaatgaaagt
    10561 tgtcataatt gctggaggaa agggaacaag aatttcatcc attacgaatg aaattccaaa
    10621 atcaatgata aaggtagaag aaaggccggt cattgagtat caagtagaac tggccaaacg
    10681 atatgggttc aacgaattta tatttataat cggacattta ggagaacaaa tcgaaactta
    10741 ttttggggat ggttcaaaat ggggtgtgtc aattcaatat tttaaagaga aagaagcatt
    10801 aggaactgcg ggtgctttaa cttttctgac agaagaatta aaagaggatt ttttcgtctt
    10861 ttatggcgat accgtgatgg atgtggattt gttgagaatg tggaattttc atcagaaaaa
    10921 acaatcggat gcaactttgt tcgttcatcc aaatgatcac ccgtatgatt cagatttagt
    10981 tgatatcgat gaggaaggca aaataaagaa ctttttcaac aaaccacatt ctcccgaatt
    11041 tattggaaga aatttagtaa acgctgctct ttttatcttt tcacctaaga ttttgcaaaa
    11101 catacaaaaa ggagttaaat tgcatatcga aaaagatgtt ttaccgaagt gtttgaatca
    11161 gggaatgaat ttatatggtt acgtttcgcc cgaatatgtg aaagatatgg gaacgcccga
    11221 cagatatatg gaagtttgtg aggatgttcg ttcgggaaaa gttgctcgaa tgaattggaa
    11281 caacccaaaa tttgctgtat ttttggatag ggatggcgtg atttcaaagg aaattgatct
    11341 actttcttct atggatcagt tagaattaat tgatggagcc ggtgaagcta tcagatatat
    11401 caatcaaaaa ggttatctgg caattgttgt taccaatcaa ccggtcattg cacgaaatct
    11461 ttgttcgctc gatgaattgg ctgaaattca tgcaaaactt gagactcttt taggtgaaaa
    11521 acag
//
LOCUS       DAOSCH010000065        11511 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3753_length_11511_cov_5.593052, whole genome shotgun sequence.
ACCESSION   DAOSCH010000065 DAOSCH010000000
VERSION     DAOSCH010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11511
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3753_length_11511_cov_5.593052"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..319)
                     /locus_tag="PLZ54_06040"
     CDS             complement(188..319)
                     /locus_tag="PLZ54_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66521.1"
                     /translation="MNIIFRHVDSIRPLKNKLYSDENGQSQSDGSMRGITGKGMSRV"
     gene            672..1742
                     /gene="gcvT"
                     /locus_tag="PLZ54_06045"
     CDS             672..1742
                     /gene="gcvT"
                     /locus_tag="PLZ54_06045"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOJ66522.1"
                     /translation="MKITPFTHFHIALGAKMTEFAGFYMPIEYTGINEEHLTVRRAAG
                     VFDVSHMGEFLVKGPHALAFLQKMTTNDVAALPLGKAQYSCFPNGRGGIVDDLLVYRL
                     DKENYLLVVNAANIEKDWNWCMQNNTENAELVNISEQVAQLAVQGPKATQILQKLTPV
                     RLSEIPYYSFAIGKFAGVEPVLLSNTGYTGAGGFELYFRPDCADTVWNAIFEAGADEG
                     IRPIGLGARDTLRLEMGYCLYGNDIDDTTSPIEAGLGWITKFVEGKNFIDRPLLEKQK
                     REGVTRKLVRFELTERGIPRHGYPIVNNENKPVGQVTSGTMLPDSKKGIGMGYVATEY
                     AQPGTKIYIQIRNKNLEGIVRK"
     gene            complement(1817..2284)
                     /locus_tag="PLZ54_06050"
     CDS             complement(1817..2284)
                     /locus_tag="PLZ54_06050"
                     /inference="COORDINATES: protein motif:HMM:NF024292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding protein"
                     /protein_id="HOJ66523.1"
                     /translation="MRINKFFILSILSLALFSCEPKYEKEYNWAYPVCGDWMVKAYLD
                     GTEIYGPFEIMTYNTSFGQDSIWIHDYATTATNGHFWSFKVKAAVDMKTLTFQTEGSI
                     NAIAGYPIVIKVSNGRVINKDSITMELEFEDDPGTIYQLAGHRETSYEEYMQQ"
     gene            complement(2298..3008)
                     /locus_tag="PLZ54_06055"
     CDS             complement(2298..3008)
                     /locus_tag="PLZ54_06055"
                     /inference="COORDINATES: protein motif:HMM:NF027719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5012 domain-containing protein"
                     /protein_id="HOJ66524.1"
                     /translation="MKKIILMTLAFVAFVFTSCEKESLGVSRVTTYATMELNGPEELF
                     WPLNSPFVDPGCKAFEGTTDISGNIEVISNVNATKGGKYSITYKVKNSDGFYAQTSRT
                     VYVYEQNAPLNGYYQSRIRRNNNGTIANRGPFTILVFGVGNDQYWIEDLLGGWYNYGS
                     GYGPAYAGPGIIKLNSDNTFEIVSAEPLAWGYPCILTENTVSTYDPSTKTIVLNTRMK
                     DTPLMLFTVTLTNPQPLN"
     gene            complement(3037..4434)
                     /locus_tag="PLZ54_06060"
     CDS             complement(3037..4434)
                     /locus_tag="PLZ54_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOJ66525.1"
                     /translation="MKRLKFYNIAAFIGLLFLSSCSESFFDINHDPNNPSEATPGLVL
                     PSALANSAYVMGGYYHALGSFWTQQYAQAPAASQWADWESYNLTNVDFDRQFTLLYAG
                     GLNDYEYVRKNTAETGNWKFYAIATLMQAYTFQVLADLYDQIPFREALKGNVIPHYDN
                     GQLVYDSLLARIDYAMSKDFTGSTVENPGSSDLIFGGNIDDWKAFANTLKLKMYLRYV
                     NVNPNTYRTEIEALLAENKFLTKDAKFTAFKEEQTGYNPFYNTFVDRLSGNVVANKTF
                     IDLLKDKNDPRMAKIFNPSVTGSLYNGIAAGDNKNHPTETSKNYATPAIKGTSPVYFF
                     SKEEVLFLIAEAQARYGTASDAESTYKTAIRASLTSLGLAADAIDIDATYPYNGIRSI
                     IEQKWIAQANKNGIEAFFDYNRTGYPNFFTRSLTSVLNGDERPKRLYFPSREVNSNPY
                     TPARVDLTEPVWWAK"
     gene            complement(4457..7609)
                     /locus_tag="PLZ54_06065"
     CDS             complement(4457..7609)
                     /locus_tag="PLZ54_06065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOJ66526.1"
                     /translation="MRKLTFLFACLFMIGVGLVNAQSLTVKGKVISDEDGQPVIGASV
                     LVKGTTTGTITGTDGSFSITVPAGNRTLVVSYIGMKSVEVPAENGMVVRLQPQTLEID
                     EVVVTAIGIKRSEKALGYSASAVSSQDITMTGNRSALNALQGKVAGVEITSASGAPGS
                     STRVIMRGYSSLGGSNQPLYVVDGVPISNGQIGSTSINGGLDFGNRANDINPEDIESM
                     TILKGGSATALYGSRAASGVILITTKKGEGLKNKPKVEVSSTSTFDSPLRLPYFQNEF
                     GQGWYDRGGGEQDLMENGSWGPRFDGKIRPWGFVVDNQQQIKPYVALPTNIKDFFDIG
                     TNYNNTVSVSNGDADKSYYISYGNVTADGIMPTDADYYNRNTVSMRGSAKFLKIFNIS
                     GSLNYVRKDMRFTETGQDQAALDGLWQSPRDISIVDLKDYNNKFNNVDNYYTIYAQNP
                     YYILNEHGNKFQENRIFGNISLDAQITPSLSATFRLGDDVANSTLKTWRAITNSARAD
                     YNDEVGRVGENAYYSSELNTDFFFNFNPKLNNDFAISVIVGHNFNQRDAQSQGSEVIG
                     LDIPKFYNLSNSSATPSIYGTLSQRRLIGVYGNLDVSFKDMLFLNITGRNDWSSTLPA
                     NNRSFFYPSISGSFLFSELIQNKDVLSYGKLRASFARTGKDADPYLIYPVLLQTSITD
                     GYRSLDFPLAGPVNGFTISNVIGNDQLQPEISQDFEIGTNLKLFKNLLDIDFTWYNKK
                     ITDLIWTATLPSSTGYTSQTQNLGEITNKGVELSLSVTPIRRKNIEWEVFANYSKNNN
                     LLKSLISGLDEIVLGGTRSFPFVARPGYELGLFKGNVPETDPQGRIVVNAQGLPVFKA
                     EQEIIGSSQNKYRLGGGTSLKIKNFRVHAAFDYRNGGKMYSRTAEIAYFTGNAQPTTY
                     NDRQPFIIPNSVQLVGGQYIENTTPIAGFTNNLNLYYNQTYKAGIGDAYALIDKTFFK
                     LRELSISYSFPKSVLKNAFINSVDLSLVGNNLFLWTPDSNLYTDPELTTFGNDLAADY
                     GEFGATPSTRSLGFNIKLSF"
     gene            complement(8641..10422)
                     /locus_tag="PLZ54_06070"
     CDS             complement(8641..10422)
                     /locus_tag="PLZ54_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOJ66527.1"
                     /translation="MLAAPNEQSLLKSYFDLTGYPPLQPKWIYGLIQSKCSYENDTMV
                     YNTLQKFEQKKLPVDAIVLDIPWFGGLKPTSPHFMGNFSWNLQNFPDPIKYINTLRGK
                     NVKTILINEPYINIDSYNYKFLKEKGWLVSKKNQSEPYVFSPFWAGDVSLFDITHPDA
                     QKWLWQQLKAEVEFGADGLWSDLVEPESPVVDGQFYLGDWRKVHNIYSLLWAKNIYDG
                     FSTDFPNKRITNLSRAGFGGIQRYGALCWSGDASKTWFALKLQIPMMTGTVLSGIPYF
                     SSDIGGFTFAHDRRDGNTIFTNIVDGRMKTEPELYIRWFQHGVFSPMLRPHSGEEQFC
                     EVFAFDSLTESITTKYLHLRYRLIPYMYSYSYKTYKKGETLIRPLFFEFSDSLARKQD
                     YQYMFGKEMLVAPVLDEGKTNQQVYLPSENQTWIDFWSNKTYRGGQTIDYNAPLDIIP
                     VFVKAGSIIPLAKNKMRAEEPDDTLTLRIYPDKTAKFEIYEDDNKTKNYQKGEFGITN
                     ITSWLCNHQLTINIDAMQGEFENKITERTWIIEIPVSAECKSIKLNNKKLTNFTFDKK
                     NGMLHFEFKADTRVKQKIQIDKFRTTY"
     gene            complement(10482..11105)
                     /locus_tag="PLZ54_06075"
     CDS             complement(10482..11105)
                     /locus_tag="PLZ54_06075"
                     /inference="COORDINATES: protein motif:HMM:NF027659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4968 domain-containing protein"
                     /protein_id="HOJ66528.1"
                     /translation="MKRFFFSFFLSILLHTGYSQFNDSLFSYSCSKNTVVVQNDNSII
                     KVEAWCDNIFRISWFPDMHNIEIDSSHSVILNQAPEVKFSVKEKHNELIIKGRLIVAK
                     ITKIPFNISFYKPDGSILLQSAGAFECDGSSKKASFKIRETDHFYGMGQKSIAVDRRG
                     EAFEVFNQHIGGYTKPYGTMQINIPYIYILQPDLEFILSISIQPDLI"
BASE COUNT         3593 a         2173 c         2047 g         3698 t
ORIGIN      
        1 catgcagaaa tccgctaaaa taaacataca taagaattta ttttacagtt aaatacatga
       61 atttttcggt ttgtaaaaaa tacattttta agaaaaattg tttgctacat atggtcaact
      121 ccaattattt gaattaaaaa acacaaaata tgggttgctt tcaggttggg tttttttgat
      181 acctgtgtca aacacgactc atcccttttc ccgttattcc tctcatcgat ccatcagact
      241 gtgattgtcc gttttcatca gaatataatt tattttttaa aggtctgatg gaatccacat
      301 gtctgaatat tatattcatt cgtgtttgtg tatgcaatac atgtgggttt cttagataaa
      361 aaaatccttt tgaacttacg gtactaaatt agttccaaca aattaatttt tttcatactt
      421 ttgtatttat taaaatttgc attgaaatta acattaaatg ccttatacta acaaatcata
      481 aacccaaaac tgatacattc caataaagtg gaaaagtttg gtcttgtcgg gcaaagtgtt
      541 tttgaatatt tttttcatga ctaactacga aaaatcataa tactccaaac gcaagtacat
      601 ccaatcgatt atcatttaat tttcaaataa aaacaagtat cgattttttt catacaaatt
      661 taaccaagct tatgaagatt acaccattta cgcattttca catcgctctt ggagcaaaaa
      721 tgaccgagtt tgcaggtttt tacatgccga ttgaatatac aggcatcaac gaagaacatt
      781 tgactgtcag acgggctgcc ggcgtttttg acgtgtccca catgggcgaa tttctggtga
      841 aagggcctca tgcattggct tttcttcaaa aaatgactac caacgatgtg gctgccctcc
      901 cgttgggaaa agcgcaatat tcctgtttcc cgaacgggag aggtggaatt gtggacgatt
      961 tgctggtgta tcgcctcgat aaggaaaatt atttgctggt ggtaaatgca gccaatatcg
     1021 aaaaagattg gaattggtgc atgcagaaca atacggaaaa tgcagagctg gtaaatattt
     1081 cggaacaggt ggctcaattg gccgtgcaag gtcccaaagc tacgcaaatt cttcagaaac
     1141 tcacgcctgt tcggctttcc gaaattcctt attactcatt tgctatagga aagtttgccg
     1201 gtgtggagcc ggtgttgctg tccaataccg gttatacggg agccggcgga tttgaacttt
     1261 atttccgtcc cgattgtgcc gatacggtat ggaatgccat ttttgaagcc ggagcagacg
     1321 aaggcattcg ccccattgga ctgggagccc gagatacttt gcggctcgaa atgggttatt
     1381 gtctttacgg gaacgatatt gacgatacca cttctcccat agaagccggt ttgggatgga
     1441 ttaccaaatt tgtggaagga aagaatttca tcgaccgtcc attgctcgaa aagcagaaaa
     1501 gagaaggtgt aacgcgaaaa ctggtacgtt ttgaacttac cgaacgaggt attccgcgtc
     1561 atggctaccc aattgtaaac aatgaaaaca aacccgttgg acaggttact tcgggcacca
     1621 tgctgcccga tagcaaaaag ggaatcggga tgggatatgt ggcaacggaa tatgcacaac
     1681 ccggtacgaa aatttatatt caaatccgaa acaagaattt ggaagggata gtgaggaaat
     1741 aaacacattg ttaagtacag gaatgaaaaa ggctgcggtt ttccgcagcc ttttcaataa
     1801 ttaatcgaca gcatgtttat tgctgcatgt attcttcgta ggaagtttca cgatgaccgg
     1861 ctaattggta aattgttccc ggatcatctt caaactctag ttccatagtt atgctgtcct
     1921 tgttgattac ccgaccatta gaaactttga ttacaatggg ataacctgca atagcattaa
     1981 ttgagccttc tgtttgaaac gtaagtgtct tcatatctac ggccgccttc actttgaacg
     2041 accaaaaatg tccattagta gcagtggtag cataatcgtg aatccagatg gaatcttgac
     2101 caaacgaagt attataagtc ataatttcaa atggcccata tatttctgtt ccatccaaat
     2161 aggcttttac catccaatcg ccgcaaacag gataagccca attatattcc ttttcatact
     2221 ttggctcgca ggaaaaaaga gccaatgata aaatagatag gataaaaaat ttatttatac
     2281 gcatagttat atctccttta gtttaatggt tgtggattag ttaatgttac cgtaaacaac
     2341 attagtgggg tatctttcat tcttgtatta agcactatgg tttttgtaga aggatcataa
     2401 gtggatactg tattttcagt taaaatacat ggatatcccc aagcgagagg ttcagcggat
     2461 acaatttcaa aagtattatc actattcaac ttaatgatgc ctgggcctgc ataagccgga
     2521 ccatatcctg aaccataatt ataccacccg cctaatagat cttctatcca atattgatca
     2581 ttacctactc caaaaacaag gatagtaaat ggaccacgat tagctatagt tccattatta
     2641 tttcgcctta tacgagactg ataataccca tttaaagggg cattttgttc atatacatac
     2701 actgttcgcg atgtttgagc gtaaaagcca tcagaatttt taactttata ggtaatggaa
     2761 tatttacctc cttttgttgc atttacattt gaaataacct ctatatttcc tgaaatgtca
     2821 gttgttcctt caaaagcttt acatcccgga tctacaaagg gggaatttaa aggccaaaac
     2881 aattcttcag gcccattcaa ttccatagta gcataagttg taactcttga aacaccaagc
     2941 gactcttttt cacaggaagt aaaaacaaaa gccacaaatg ccaaagtcat taatattatt
     3001 tttttcataa cgaattaatt ttaattgtta attgttttac ttagcccacc atacaggttc
     3061 tgtaagatcc actcttgcag gagtataggg gtttgaattg acttctctgc tggggaagta
     3121 caagcgttta ggtctttcat ctccattcaa aacagaagta agtgaacgag tgaagaaatt
     3181 gggatatccc gtacgattgt aatcgaaaaa tgcttctatt ccgtttttat tggcttgtgc
     3241 gatccatttt tgctcaatta tcgaccgaat tccattatag ggataggttg catctatgtc
     3301 gatggcgtct gctgctaaac cgagacttgt caaggaagcc cgtatggcag ttttataggt
     3361 gctctcagca tcagaagccg tcccataacg agcttgagct tcagcaatca aaaagagtac
     3421 ttcttccttt gagaaaaaat aaacgggact tgtaccctta atagctggag tcgcataatt
     3481 tttactggtt tcagtggggt gatttttatt atcacctgca gcaataccat tatataagct
     3541 accggtaacc gaaggattaa aaatttttgc cattcgtgga tcgttcttat cttttagcaa
     3601 gtcaataaaa gttttattgg ctacaacatt acctgacaat cgatcaacaa aagtattgta
     3661 gaaaggatta tatccggttt gttcctcttt aaaggcagta aacttggcat cttttgtcaa
     3721 aaacttattt tcggccaaca aagcttcaat ctctgttcta tatgtgtttg gattaacatt
     3781 cacatatcgt aaatacattt ttaatttcaa tgtattggca aatgctttcc aatcatctat
     3841 atttccgcca aaaataagat cagaagagcc gggattttca actgtgcttc cggtaaaatc
     3901 tttagacatt gcataatcaa tccgtgcaag taggctatca taaaccagct ggccattatc
     3961 ataatgaggt ataacattcc cctttaaagc ctctctaaat ggaatttgat catacaaatc
     4021 agccaatacc tgaaaagtat atgcctgcat taaggtagcg atggcataga atttccagtt
     4081 cccagtctcg gcagtatttt tacgaacata ttcgtaatca tttaatccgc ctgcatataa
     4141 aagtgtaaat tgacgatcaa agtccacatt tgtcaaattg taggattccc aatcagccca
     4201 ttgagacgct gcaggagcct gagcatattg ttgtgtccag aaacttccca aagcatgata
     4261 ataaccaccc attacatagg ccgaattagc caaagcactt ggaagtacta atcccggagt
     4321 tgcttcggat ggattgtttg ggtcatgatt tatgtcaaaa aagctttcgc tgcaactgct
     4381 taagaatagc aagccaataa aagcagctat attataaaat tttaatcgtt tcatatataa
     4441 ttcgttttta tgttttttag aaactcaatt taatattaaa tcccaaactt cttgttgatg
     4501 gagttgcccc gaattcacca taatcggcag ccaaatcgtt cccaaaagta gttaattcag
     4561 gatccgtgta caaatttgag tctggagtcc acaagaacag gttatttccc accaaagaca
     4621 aatcaactga attgataaaa gcattcttca aaaccgattt cgggaaagaa taggaaatgc
     4681 tgagttctct cagtttaaag aatgttttgt caattaacgc ataagcatcc ccaattcctg
     4741 ccttgtaagt ttgattgtaa tacaaattca agttgttagt aaaacctgcg ataggagtgg
     4801 tgttttcaat atattgtcct ccaactaact gtacagaatt cggaataata aatggttgtc
     4861 tgtcgttgta ggtagtaggt tgtgcattac cagtaaagta agcaatttct gctgtgcggg
     4921 aatacatttt tcctccattt ctataatcaa atgcagcatg taccctgaaa tttttaattt
     4981 tcaaagaagt tccgccaccc agtcggtatt tattttgaga agacccaata atttcctgtt
     5041 cggctttgaa aacaggtaat ccttgtgcat ttacaacaat acgtccttgt ggatcggtct
     5101 caggaacatt gcctttaaaa agtcccaatt catagcccgg acgagccaca aacgggaagc
     5161 ttcttgttcc tcccaacaca atttcatcca aaccagaaat taaagattta agtaaattgt
     5221 tgtttttgga ataattggca aaaacctccc attctatatt ttttcttcgt ataggtgtca
     5281 cacttaaact cagttcaact cccttattgg taatttctcc gaggttttga gtttgtgaag
     5341 tataaccggt agaagatgga agggttgcag tccaaattaa atcagtgatt tttttgttgt
     5401 accatgtgaa atctatatcc aataaatttt tgaaaagttt caaattggta cctatttcaa
     5461 agtcttgaga tatttcgggt tgtaattggt cgttaccaat aacgttggaa atggtaaaac
     5521 cattcacagg accagccaaa ggaaaatcca aagaacgata gccatccgta atactagttt
     5581 gaagcagtac aggataaatt aaatatgggt cagcatcttt accggtacga gcaaagctgg
     5641 ctctaagttt tccataagaa agtacatctt tgttttgaat caattcagaa aacaaaaagc
     5701 ttccgcttat gcttggataa aagaatgaac gattatttgc tggtaaagta gatgaccagt
     5761 catttctacc ggttatgttc aaaaacaaca tatccttaaa gctcacatcc aaattgccat
     5821 aaacgccaat taatctacgt tgagacaaag ttccataaat agaaggagta gcagaactgt
     5881 ttgacaaatt atagaatttg ggaatatcga ggccaattac ttcagaacct tgagattgag
     5941 catcgcgctg attaaaatta tggcccacaa tgacactaat cgcaaaatca ttatttaatt
     6001 ttggattgaa attaaaaaag aaatcggtat tcaattcgga tgagtaataa gcattttctc
     6061 ctactctacc cacttcatcg ttgtaatcag ctcttgcact gttggttatg gctctccatg
     6121 ttttcaatgt actgttggcc acatcatcgc ccaatctgaa ggtggcactc aatgatgggg
     6181 ttatctgcgc atcgaggctg atatttccaa aaatgcggtt ttcctgaaat ttattgccat
     6241 gttcattcaa tatatagtaa ggattttggg catagattgt gtaataatta tctacattgt
     6301 tgaatttgtt gttgtaatct tttaaatcaa caatactgat atcgcgtggc gattgccaaa
     6361 gtccatccaa tgcagcctga tcctgtccgg tttctgtgaa tctcatatcc tttcgaacat
     6421 aattcaacga accggaaatg ttaaaaattt tcaggaactt tgctgaaccg cgcatggaaa
     6481 ctgtattacg attataataa tcagcatcgg ttggcataat tccatcggct gttacatttc
     6541 cgtaagaaat ataatagctt ttatcagcat cgccatttga aacagaaacc gtattattat
     6601 aattggtacc gatgtcgaag aaatctttta tattggttgg taaagccaca taaggtttta
     6661 tttgttgttg gttgtcaact acaaagcccc atgggcgaat cttgccatca aagcggggtc
     6721 cccagcttcc attttccatc aaatcttgtt ctccgccacc tctatcgtac caaccctgac
     6781 caaattcgtt ctgaaaatag ggcaaacgaa gtggagagtc gaaagtagaa gttgaagaaa
     6841 cttcaacttt aggtttattt tttaatcctt cacctttttt ggtagttatc aaaataacac
     6901 ccgaagctgc acgagatcca tacaatgcag tagccgaacc gcctttaagt atagtcatcg
     6961 attcaatatc ttccggatta atatcattgg cacggttacc aaaatccaat cctccattta
     7021 tgcttgtcga tccaatttgt ccattgctga tgggaactcc atcaacaaca tacaaaggtt
     7081 gattgcttcc tcccaatgaa gagtaaccac gcatgataac gcgagtagaa gatccgggag
     7141 ctccagaagc ggaagtaatt tcgacgcccg ctaccttacc ttgcaatgca tttaatgcac
     7201 ttctgtttcc ggtcatagta atgtcttgtg agctcactgc cgaagcggaa tatcccaaag
     7261 ctttttctga gcgtttgatg ccgattgccg taaccacaac ttcgtcaatt tccaacgttt
     7321 gaggttgcag ccttactacc atcccgtttt cagctggaac ttccactgat ttcattccaa
     7381 tgtaggaaac aaccagtgtt cggttgccag ccggcacagt aatggaaaaa cttccatcag
     7441 tcccggtaat tgtgccagta gtggtacctt ttaccagtac agaggcgcca ataacaggtt
     7501 gcccatcttc atccgaaatc acctttcctt tgactgtcag agattgggcg ttgaccaaac
     7561 caactcctat cataaatagg caggcaaata agaatgttag ttttctcata aattttacat
     7621 ttttgtttat taatcttatt atttattatt ttcacctata gcagggaaaa cgtgctggca
     7681 aaaataaaat attaaaaaat aaaaaacaag tgtttttgat gacaaataga aaaaatattc
     7741 aatttttccc tttcattttg cagtttattg ccaaaattcg tatccctttt taagtaccat
     7801 cgataatttt acaaataaaa atatctttgc aaatacctgt gaataaatgt cttacttatt
     7861 tcttaaaatt ttctgttaaa aaaatattgc cataagtaaa caaatttcat tcaacaaaaa
     7921 attttttgtt aaaatcaaat atttaacatt ttactcagca aaaaaatgac agttgataca
     7981 aaaatcacta cattttatgt taatttttaa tcaataaaat tgaaagtata tctaacagtt
     8041 ggctcgaaaa cgttagaaaa taaattttat aacacttctt tatgtttttt tccttaggac
     8101 ataacaaaaa aattgtaaaa acgtttatat tttttatttt cagatattta ataacggggc
     8161 tttcaccaaa cctcacttac ttttttgtga ggtcatttaa aaacagacca ttgcttaaat
     8221 cgtaattttt acaaaggaat tacaggcgat aaaatgtaat gcttgcagca gctgcatgta
     8281 tcttcaaaag aatgatgaat aaatgggaat tatctacttg gctcgaattt aaaatacaaa
     8341 ttgttgactt gataaaccct atgttttacc aagccaacaa tattttaaga caaaaatggg
     8401 gtctttaagg atcgacgttt taacaaaaaa cttcttttat gaatatccaa tagatgttca
     8461 aagggttttt cgttgttgta gtaggtttct ctgaagttac tcagaaatgg ttgttggaga
     8521 atttgatacg caaatctaag caaaacccat ttgtttataa aagctgctaa gatttggatt
     8581 ctttctgatt agattcttta aaccatacag taaaatcgaa acggatttct aatgtgaaat
     8641 ttaataggtt gttctaaatt tatcaatctg aattttttgt tttactcttg tatcggcctt
     8701 gaattcgaaa tgcaacattc catttttttt atcaaaagtg aaatttgtta atttcttatt
     8761 gttgagtttt atacttttac attcagcgga gacgggaatt tctataatcc atgtacgttc
     8821 tgtaatctta ttttcaaatt ctccctgcat tgcgtcaata ttaattgtaa gttgatgatt
     8881 gcacaaccaa ctggttatgt ttgtaattcc aaattctcct ttttgataat ttttggtttt
     8941 gttgtcatct tcgtatattt caaacttagc tgttttatca ggataaatac gtaaagtaag
     9001 tgtgtcatcg ggttcttcag cgcgcatttt atttttagct aatggaatta tagaaccggc
     9061 tttaacaaaa accggaataa tgtcaagtgg agcgttataa tcgatagttt gtcctcccct
     9121 ataagtttta ttactccaga aatcaatcca tgtttggttt tctgaaggca agtaaacctg
     9181 ttgattggtt ttaccttcgt caagtacagg agctacaagc atttctttgc caaacatata
     9241 ttgataatcc tgtttacgag ctaaggagtc tgaaaattca aaaaataacg gacggattag
     9301 agtttcgcct tttttgtagg tcttgtatga atacgaatac atatacggta ttaaccgata
     9361 tctcaggtgt aaatattttg ttgttatact ttcggtcaga ctgtcgaatg caaaaacttc
     9421 acaaaactgt tcttcaccac tgtgtggacg taacattggc gaaaatacac catgttgaaa
     9481 ccaacgtatg tacaattcag gttcggtttt cattcgtcca tcaacaatat ttgtaaaaat
     9541 ggtattgcca tcacgcctgt cgtgggcaaa ggtaaatcca cctatatcgc tggaaaaata
     9601 aggtatccct gaaagcacag tacctgtcat catgggtatt tgtaacttaa gagcaaacca
     9661 ggttttagat gcatcgccac tccagcacaa tgctccataa cgttgaatgc cgccaaaccc
     9721 tgccctgctt aaatttgtga ttcttttgtt tggaaaatca gttgaaaaac catcataaat
     9781 gttttttgcc cacaataaac tataaatgtt atgtactttt ctccagtcgc ccaaataaaa
     9841 ttgtccgtca acaacaggac tttcgggttc aacgaggtcg ctccaaagtc catcggcacc
     9901 gaattcaact tccgctttca gttgttgcca aagccatttt tgggcatcgg ggtgagttat
     9961 atcaaaaagt gaaacatcgc ctgcccaaaa cggactaaaa acataaggtt cggattgatt
    10021 tttttttgaa acgagccatc ctttttcctt gagaaattta taattatatg aatcgatgtt
    10081 gatatatggc tcatttatta aaatcgtctt gacatttttt ccacgtaatg tatttatata
    10141 tttgattgga tcgggaaaat tttgtaggtt ccacgaaaaa ttacccataa aatgtggcga
    10201 agttggtttg agcccgccaa accatggtat atcaagtaca atagcatcga caggcagttt
    10261 tttctgctca aatttctgaa gcgtattata aaccattgtg tcattttcgt acgaacattt
    10321 gctctgaatc aaaccataaa tccatttagg ctgcaagggt ggatatccgg tgagatcaaa
    10381 ataacttttc agcagtgatt gttcattagg tgcggctaac agatagtatg tgaaagaacc
    10441 gttttcgacc tcataatacc attctgtcgg attagttttg cctaaatcaa atctggctgg
    10501 atactgattg acaaaataaa ctccaaatcc ggttgtagaa tatatatata tggaatattt
    10561 atctgcatgg taccataagg cttagtatag ccaccaatat gctgattaaa aacttcgaaa
    10621 gcctcgccac gacgatcgac ggcgatgctc ttctgtccca ttccataaaa atgatctgtt
    10681 tcccgtattt taaacgaagc ctttttggaa ctaccatcac attcaaaagc accagctgac
    10741 tggagcaata tagacccgtc aggcttgtaa aagctaatat taaatggtat tttggtaatt
    10801 ttcgcaacaa tcagtctgcc tttgataata agttcattgt gtttctcttt cacagaaaat
    10861 ttgacttcgg gcgcctgatt taaaataact gaatgcgatg aatctatctc tatattatgc
    10921 atgtcgggaa accacgaaat gcgaaatata ttatcgcacc aggcttccac tttgataatt
    10981 gagttatcat tttggactac gactgtattt tttgaacagg agtatgaaaa caagctgtcg
    11041 ttgaactgtg aataacctgt gtgtaaaagg attgataaaa aaaagctaaa aaaaaatctt
    11101 ttcatttgga ttaaatttat tcttgtctga cggatgaatc agttgggtct acaaatatat
    11161 aactttaaat tttcagagaa taatactatt ctaacaattt atgtacattt tctggccctg
    11221 aagatattta tgtaaaattg aatcccctca taaaacttaa tttttctaaa ggcagaaaaa
    11281 atcaagtttt atgaggggct gaagtaatgt ataatcaagt aggtagggga atcgcccctc
    11341 tctcacatca atgtgcatgc tctcgcacac ggcggtttct tttaactgtt taacctctcg
    11401 taatttaatg tgagatttta caaacctccc atcaaatcgt gaattaaggc tcgttcctgt
    11461 tcgcctgctt cttttacttg caacgccttt atcgcctgag ctccgcttac g
//
LOCUS       DAOSCH010000066        11301 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3834_length_11301_cov_5.565979, whole genome shotgun sequence.
ACCESSION   DAOSCH010000066 DAOSCH010000000
VERSION     DAOSCH010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11301
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3834_length_11301_cov_5.565979"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2651)
                     /locus_tag="PLZ54_06080"
     CDS             complement(<1..2651)
                     /locus_tag="PLZ54_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOJ66529.1"
                     /translation="MNTLSINKLYFKDTSFANLMTHRIYNILLYASKYDAFVLEEDGR
                     IDEQIFNEYTALNLRYPPRFTLVSNEEEANELLRTRTFELIISMPSGDSINPFEWAKD
                     VKKEYPEIPIVVLTPFSSSISQRLAHEDLSAIDYVFSWLGNSDILLAIIKLIEDRMNV
                     EEDVNSVGVQVILFVEDSIRWYSSIVPHLYKYVFKQSRSFMTEALNEHEQMLRMRGRP
                     KILLARSYEEAISIYNKYSQNMLGVITDVSFPEKNVKDKKAGLHLCSEIRRNDKFIPL
                     IVVSTEEENRQTAEELNAVFLNKMSKTLFMELREKITDNFWFGDLIFFNPQTGVEEAR
                     VHNLRSLQDCIFKVSDEALYYHVSRNNISKWLYSRAMFPLAEFLKNIQVEPGMSENMK
                     NVRQIIFDAIVHYRKIKNRGIVAVFQRDRFDMYSNFARIGEGSLGGKGRGLAFLDAMI
                     KRNESLDKFENVQIAIPRTVVLCTDIFSDFMEMNQLYPIALSDIPDEEILAHFLKARL
                     PQKLLADLYAFLGAVSKPIAVRSSSLLEDSHYQPFAGVYSTYMVPFDANSKTHTLQMI
                     TEAIKAVYASVFYHDSKTYMAATKNLIDEEKMAIVLQEICGSAYENRYYPSFSGVARS
                     LNYYPIGNEKPEDGIANIAMGLGKYIVDGGKTLRFSPAYPNNVLQTSTLEFMLSETQT
                     FFNALDLNMVQFVPQVDDGFNLLRINVQDAIKDGTLQYLASTYNVSDQMVYDGLYEGG
                     RKIITFANILQHNVFPLAELLREILRIAQEGMGRPVEIEFAVNLDYSPKKQHSFYLLQ
                     VRPIVDNKEIINEDISAIPEEETIVQSSNALGNGIMTDIYDLIYVKQEAFNGSNNRLI
                     MYDIEKLNRQMVKEEKYYVLIGPGRWGS"
     gene            2956..4290
                     /gene="gdhA"
                     /locus_tag="PLZ54_06085"
     CDS             2956..4290
                     /gene="gdhA"
                     /locus_tag="PLZ54_06085"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOJ66530.1"
                     /translation="MDIEKFMQVLSAKHPNENEYLQAVREVLTTINDVYSQHPEFEKA
                     KIAERLVEPDRIFTFKVPWVDDQGNVQVNIGYRVQFNNAIGPYKGGLRFHPSVNLSIM
                     KFLGFEQIFKNALTTLPLGGAKGGSDFNPKGKSDAEVMRFCQAFMTELWRYIGPETDV
                     PAGDIGVGAREIGYLYGMYRKLAHENTGVLTGKNLEFGGSLIRPEATGFGALYFVREM
                     LQRAGMDIRGKTVAISGFGNVAWGAALKASQMGAKVVSISGPDGYVYDENGISGEKID
                     YMLELRASCNDIVSPYAEKFGVPFIPNRRPWELKVDIALPCATQNELNEEDALNLIEN
                     KVRCVAEVSNMGCTPEAIDLFIKNKIMYAPGKAVNAGGVSVSGLEMTQNAMHISWTAE
                     EVDKQLHQIMNKIHEQCVNFGKEEDGYINYLKGANVAGFIKVARAMLAQGIV"
     gene            complement(4427..5890)
                     /locus_tag="PLZ54_06090"
     CDS             complement(4427..5890)
                     /locus_tag="PLZ54_06090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ66531.1"
                     /translation="MKPINCFIVYDDAVQAEITIENLLQSELVNAVYLLSKRENIQVN
                     EKCFPILIDSLYSSNTIRKIAEKSDTPYSLVYLKNTPLHLGQFALERFFQIAQDTKAG
                     MVYSDYYQVSGGVRSKLPLIDYQTGSVRDDFNFGSVLFFNAEVLKQASAQMNENFLHA
                     GLYDARLRISQKAELVHIDEYLYSEVESDNRKSGEKLFDYVDPKNRQRQLEMEHACTE
                     HLKNIGAYLKPQFKTILFDENNFEVEASVIIPVKNRATTIEDAVRSALTQKTSFPFNV
                     IVVDNHSTDGTSEIVEKLAKEDSRVRHIIPQREDLGIGGCWNVAVHSPFCGKFSVQLD
                     SDDVYQDENTLQIMVNAFYEQNCGMVIGSYTITDFEMNVIPPGLIDHREWTPENGRNN
                     ALRINGLGAPRAFYTPLLRKIKVPDSNYGEDYALGLYFSREYQIGRIFQSVYLCRRWR
                     DNSDAELDIVTLNTYNHYKDKIRTIEIKARQQINKLS"
     gene            complement(5932..7914)
                     /locus_tag="PLZ54_06095"
     CDS             complement(5932..7914)
                     /locus_tag="PLZ54_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HOJ66532.1"
                     /translation="MRTNLSSKITMTRIPRRYYQPDDVFELSRVTRLEKIPTEIFDTE
                     KEGSAKVAEEIAALIKTKQAAHEKCVLGLTSGKSMVGIYAELVRLHKKENLSFNNVVV
                     FLLFEYYPLTGKLKGSYGQLKNMLLDHVDIPAENIFYPDGTMDKHQIMQACAEYEQKI
                     AALGGIDLQLLGIGRSGNIGFNEPGSLTNSQTRLILLDTLSREDAAEFFSNAEMVPVS
                     AITLGISTILKAKRIVLVAWGEHKSTVVKNAVEGPMSETIPASYLQLHSNVKIVIDLH
                     AASELTRISMPWLVTSCEWDSKLIRRAIVWLCMKLKKPILKLTNKDYNDNGLSELLAI
                     YGSAYNVNIRIFNDLQHTITGWPGGKPNADDSNRPERALPYPKRVLIFSPHPDDDVIS
                     MGGTFKRLIDQNHKVHVAYQTSGNIAVADHEVIRFLSFVKGFKDLFDSNNDVIQAKYE
                     AMKNFLLYEKKENDMDTPEILEVKALIRRGEAKAACRYLGLSSQNIHFLNLPFYETGT
                     VKKNDLSQKDIDIIKDLLQQIQPHQIYAAGDLADPHGTHKICLDAVLAAIDELKDEEW
                     MKDCRVWMYRGAWKEWEIDHIEMAVPLSPEELKNKRNAILRHQSQMESAPYLGNDERL
                     FWQRSEERNRATAELYTQLGLASYEAIEAFVRYKPV"
     gene            8073..8924
                     /locus_tag="PLZ54_06100"
     CDS             8073..8924
                     /locus_tag="PLZ54_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004305110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOJ66533.1"
                     /translation="MNIIADSGSTKTHWCVIEGENTVKEFYTKGINPFYQTKEEILAE
                     LKENLYPRIMQSTIDGVHFYGAGCAFAEQKKMLSEIFRDILGKELVVTIESDLLGAAR
                     SLFGKESGIACILGTGSNSCYYNGRGIEKNVPPLGFILGDEGSGAILGKKLTSDALKN
                     QLPPDLVSKFMKEYDLTLEKVLDHVYKKPFPNRYLAQFVPFIKENIQYPPLYNIVYSS
                     FNDFFNRNICQYPHSEKTPLGFVGSVAFHFADILQTIARERHFRIIDIAQNPMKGLIK
                     YHSQKNK"
     gene            8937..9761
                     /gene="murQ"
                     /locus_tag="PLZ54_06105"
     CDS             8937..9761
                     /gene="murQ"
                     /locus_tag="PLZ54_06105"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HOJ66534.1"
                     /translation="MAFEKITEQNSLHNDLDKKDIRQLLVEMNEEDKKVPFAVERAIP
                     QIEQLIEKLVERMKIGGRLFYIGAGTSGRLGVLDASEIPPTFGMPPHLVIGVIAGGDT
                     ALRNAVEFAEDDMEQGWEDLKKHNISSIDTVVGITASGTAPYVMGALRKAKQHGILTA
                     SIACNPDSPVSHEADIKIELIVGPEFVTGSTRLKSGTAQKLTLNMISTVTMIKLGRVK
                     GNKMVNMQLTNSKLIDRGTRIIVEELNIDYEQARNILLIHGSVKKAIDAYKIQNKI"
     gene            9915..>11301
                     /locus_tag="PLZ54_06110"
     CDS             9915..>11301
                     /locus_tag="PLZ54_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOJ66535.1"
                     /translation="MKKILLLFAVLFLSLQVSLAQKVTVTGTVTSKEDGLPIIGASVL
                     EKGTTNGTITNYNGVYTISVPIGATLVFSYVGMDKVERKVTGEGKIDVIMNPTAIAID
                     EVIVTAMGVKAEKKKLNFAVQSVDAEAITDGRSPNFVNSLQGKISGVSVTSSGGSPNA
                     GSQILLRGISSINPSQNNEPLFILDGMVLSGGGSAAADINPSDIENVTVLKGAAASAL
                     YGQNAANGAIMITTKTAKAGKMSLSANASIQSDTPTRLLELQSIYGPGALGFYRPQES
                     GGYGGWGPLLDKDEPVYNNVKNYFQSGLYQKYDFSINGGSDKFQSYASVNYSRHDGIV
                     PKDYLDKMGLLLKGNYEINNKISANFMTNIVKNSYRGAGSISAIYSWPINDDITNYKN
                     SDGSIRYRYISIPKKENSPISPLWSRYMDYGLNSSTRNLLQGSLIYKPLKGLEITGRL
                     SLDQNNYMYDGY"
BASE COUNT         3629 a         2206 c         2134 g         3332 t
ORIGIN      
        1 gagccccatc gtcccggacc tatcaacaca taatattttt cttctttaac catttgccgg
       61 ttcaattttt caatgtcgta catgatcaac cgattatttg agccattgaa agcttcctgt
      121 tttacgtaaa tcaaatcgta aatatcggtc ataataccat tacccaaagc attggaactt
      181 tgaacaatag tttcttcttc gggaatagca ctgatatctt cattgataat ttccttgttg
      241 tcgactatcg gcctaacctg caacaaataa aacgaatgtt gctttttggg tgaataatcc
      301 aaatttacgg caaattcaat ttcaaccggt cgtcccatgc cttcctgcgc aatccgcaaa
      361 atttctcgca acaattcggc caaaggaaaa acattgtgtt gcaatatgtt ggcaaaagtt
      421 atgatttttc ttccaccttc atataagcca tcatacacca tctgatccga cacattgtag
      481 gttgaagcca gatattgaag tgtcccgtct ttaatggcat cctgcacgtt gatacgcaaa
      541 aggttgaagc catcatcaac ctgaggaacg aactgtacca tattcaaatc caatgcgttg
      601 aaaaacgtct gtgtttcgct taacataaat tccagcgtac tggtttgcaa aacattattg
      661 ggataggcgg gagaaaatcg aagagttttg cctccatcga caatgtattt tcccaatccc
      721 attgcaatat tggcaattcc atcttcaggc ttttcatttc caattggata atagttcaac
      781 gaacgggcta ctcccgaaaa agaaggataa taacgatttt cgtaagcgct gccacagatt
      841 tcctgcaaca caatggccat tttttcttca tcgatcaaat tcttggtggc tgccatgtaa
      901 gttttactgt cgtgataaaa aaccgaggca taaacagctt taatggcttc cgttatcatt
      961 tgcagtgtat gagttttgct gttggcgtca aaaggcacca tataagttga atatactcct
     1021 gcaaaaggct gataatgaga atcttccaac aaacttgagg atcttacggc aatgggtttg
     1081 cttacagctc ccaaaaaagc ataaagatcg gctaaaagtt tctgtggcaa tcgagctttt
     1141 aaaaaatgtg ccagaatttc ttcgtcggga atatcggaca aagcaatggg atagagctga
     1201 ttcatttcca tgaagtcgga aaaaatatcg gtgcacagta cgaccgttct gggaatggca
     1261 atctgaacat tttcgaactt atcgagactt tcgttgcgtt tgatcatggc atccaaaaaa
     1321 gccaaccctc ttcccttgcc tcccaatgaa ccttcgccaa tgcgggcaaa attagaatac
     1381 atgtcgaacc tgtcgcgttg aaaaacagct acgattccac gattttttat ttttcggtaa
     1441 tgaacaatgg catcaaaaat aatttgacgt acatttttca tgttttcgga cattccgggt
     1501 tcaacttgaa tatttttcag aaattctgcc aacgggaaca tagctctcga atacaaccat
     1561 ttggagatat tgttacgaga aacatggtaa tacaaagctt cgtcgctcac cttaaaaata
     1621 cagtcctgta aacttctcag attgtgaacg cgtgcttctt ccaccccggt ctgaggatta
     1681 aaaaatatca ggtcgccaaa ccaaaaatta tcggtgattt tttcccgcaa ctccataaaa
     1741 agcgttttcg acattttgtt cagaaaaaca gcattcaatt cttctgcggt ttgtcgattt
     1801 tcttcttcag tagaaaccac aatcagcgga ataaatttgt catttctccg tatttcgctg
     1861 cataaatgta gaccggcttt tttgtctttg acattttttt ccggaaaact gacatccgta
     1921 atcacgccaa gcatattttg gctatatttg ttataaatgg aaatagcttc ttcataagag
     1981 cgtgccaaca aaatttttgg gcgccctctc atgcgtaaca tttgctcatg ttcgttcaaa
     2041 gcttcagtca taaatgaacg cgactgctta aaaacgtatt tgtataaatg aggaacaatg
     2101 gatgaatacc agcgtatgga gtcctcaaca aaaagaataa cctgaacacc aaccgaattg
     2161 acatcttctt ccacattcat tctgtcttca atcagtttga tgatagccaa caaaatatct
     2221 gaattaccca accaactgaa aacataatcg atggcactca aatcctcgtg tgccaatcgt
     2281 tgagaaatgg atgaagaaaa aggagtaagc accacaatag ggatttcggg atattctttt
     2341 tttacatctt ttgcccattc aaaaggattt atactatcgc cactcggcat ggaaataatc
     2401 agttcaaaag tacgggtacg aagcaactca ttggcctcct cctcattcga aacaagtgta
     2461 aaacggggcg gataacgcaa attcaaagct gtatattcgt tgaaaatttg ttcatcaatg
     2521 cgtccgtctt cttccagcac aaaagcatcg tatttactgg cataaagtaa aatattataa
     2581 atccgatgcg tcattaagtt ggcaaaagaa gtgtccttaa aatataactt attaatactt
     2641 aaagtgttca taaagaaatg ttttcagtta ttttgaagcc aatgaatctc aataaaaaaa
     2701 tcaactgaac ttcaaaacac aagtttatcg aaatattttt tcaaaattag ctcaaaaagt
     2761 ttgataatcc caaataaaaa ttttaaactt gcaaagaaat aaaatgacag aaaaatatcg
     2821 aatgaaatca aaattttcaa gaaaaaccaa caactttatg cttgtcaacc aagaaataaa
     2881 tgacaatatg taaaaatttt caaaaatttg agctacacaa atctatattt caacaaaaaa
     2941 ctaaaatcta atattatgga catagaaaaa tttatgcagg ttctttcggc gaaacatccc
     3001 aacgaaaacg aatatttaca ggccgtaaga gaagtgctta ccaccattaa cgatgtgtat
     3061 tctcaacatc ccgaatttga aaaggcaaaa attgcagaaa ggttggttga acctgaccga
     3121 atttttactt ttaaagttcc ttgggttgac gatcagggga atgttcaggt caacatcggg
     3181 tatcgtgtgc aattcaacaa tgccatcgga ccttacaaag gcggtttgcg ttttcaccct
     3241 tccgtgaatc tttccatcat gaaatttctt ggttttgaac aaatatttaa aaatgcgctg
     3301 acaactttac ctctgggagg tgccaaagga ggttccgatt tcaatccgaa aggaaaatcg
     3361 gatgctgaag tgatgcgttt ctgtcaagct ttcatgaccg aattgtggcg atatataggc
     3421 cccgaaacgg atgtgccggc aggagacatt ggtgttggag ctcgtgaaat aggctatctg
     3481 tatggaatgt atcgcaaact tgctcatgaa aatacgggag ttttaaccgg aaaaaatttg
     3541 gaatttggcg gttctctaat tcgacctgaa gcaaccggat ttggagctct ttattttgta
     3601 agagaaatgc ttcaaagagc aggtatggat ataaggggaa aaaccgtagc catctccggt
     3661 tttggaaatg tggcatgggg agctgcgctg aaagcatcac aaatgggtgc aaaagttgtt
     3721 tccatttcgg gcccggatgg ttatgtatat gacgaaaacg gtatttccgg cgaaaaaatt
     3781 gattatatgt tggagcttcg ggcaagttgt aacgacattg tttctcctta tgccgaaaag
     3841 tttggcgttc catttatacc aaatcgacgt ccgtgggaat taaaagtgga cattgccctg
     3901 ccttgtgcca cacaaaacga actgaatgaa gaagatgcgc tgaatctgat tgaaaataag
     3961 gttcgatgtg tggccgaagt ctccaacatg ggctgtacac ccgaagccat tgatttattt
     4021 atcaaaaaca aaataatgta tgctccagga aaagctgtaa atgccggcgg cgtgtcggtt
     4081 tcgggtttgg aaatgacaca aaatgccatg cacatttctt ggacggcaga agaagtggac
     4141 aaacaattgc atcaaatcat gaacaaaatt cacgagcaat gcgttaactt tggaaaagaa
     4201 gaagacggat atattaatta cctgaaagga gcaaatgttg caggattcat aaaagttgcc
     4261 agggccatgc tggctcaggg aatcgtttaa tttttctaca tgtttacaat tcaaaaaacg
     4321 aaaggaaatt attttttctg tttgtgaaat aatgatttcg aaaaactaaa aaggaggaaa
     4381 atcatttttg atctctcctc ctttttgcat taagaatttt catttttcag gacaatttat
     4441 tgatttgctg ccgagctttg atttctatgg ttctaatttt atccttataa tgattgtacg
     4501 tgttcaaagt aacaatatca agctcagcat cagaattatc gcgccaacga cggcaaagat
     4561 aaaccgattg aaaaattctg ccaatttgat attcacggct aaaatacagt cctaaagcat
     4621 aatcttcacc ataattggaa tcgggaactt taatttttct cagtagcggt gtataaaaag
     4681 ctcttggtgc acccaacccg ttaattctca gggcattgtt tcttccgttt tcaggagtcc
     4741 attccctgtg atcaatcaag cccggcggta tcacattcat ttcaaaatct gttatggtat
     4801 aagaaccgat aaccatacca caattttgct cgtaaaaagc atttaccatt atttgaagcg
     4861 tattttcatc ctgataaaca tcatcactat ccaactgaac ggagaatttt ccgcaaaaag
     4921 gagaatgaac agcaacattc caacaaccgc caatacccaa atcctcccgt tgaggaatta
     4981 tatgccgcac tcgactgtct tcttttgcca atttttccac aatttccgaa gtcccatcgg
     5041 tagaatgatt atccaccaca atcacattga aagggaaaga cgttttttga gtcaaagccg
     5101 aacgaacggc atcttcaatg gtggttgccc tgttctttac tgggatgatt acggaagctt
     5161 ccacctcaaa attgttttca tcgaaaagaa ttgttttaaa ctgtggcttg agataggcac
     5221 cgatattttt caaatgttcg gtgcaggcat gctccatttc caactgacgc tgacgatttt
     5281 tcggatcaac ataatcaaac aacttttctc cactttttcg attatccgac tctacctccg
     5341 aatacaaata ctcatcgata tgaaccaatt cggctttctg tgaaattctt aatctggcat
     5401 catataaacc ggcatgaaga aaattttcgt tcatttgggc agaggcctgc ttcaacacct
     5461 ccgcattaaa aaacaaaacg gagccaaaat taaaatcgtc gcgaacgctt ccggtctgat
     5521 aatcgatcaa cggtaatttg ctgcgaacgc ctcccgacac ttgataataa tcggaataaa
     5581 ccattcccgc ctttgtatcc tgagcaattt gaaaaaaacg ttccaaagca aactgtccca
     5641 gatgtaaagg agtatttttc aaataaacaa gtgaataagg cgtatcggat ttttcagcaa
     5701 ttttcctaat tgtattgctg ctgtaaagcg aatcgatcaa aatcggaaaa catttttcgt
     5761 tgacctgaat attttctcgc ttggaaagca aatacacagc attaaccaat tccgactgca
     5821 acaaattttc tatggtaatt tcagcctgaa cagcatcatc gtaaacaata aaacaattaa
     5881 tgggtttcat aacaaatatt taataagttg atttgtaacg cagacaaata attatactgg
     5941 cttataccgg acaaaggctt caatggcttc ataagaggcc aaacccaact gggtgtacaa
     6001 ttcggcagta gcccgattcc tttcttccga acgttgccag aaaagtctct catcgtttcc
     6061 caaataggga gcactttcca tttgggattg atgacgcaaa atagcatttc gcttgttttt
     6121 caactcttcc ggacttaagg gaacagccat ttcgatatga tctatttccc attctttcca
     6181 tgcaccacga tacatccaaa ctcggcagtc tttcatccat tcttcatctt tcagttcgtc
     6241 gatagcggcc aatacggcat ccaagcaaat cttgtgagtt ccgtgagggt cggccaaatc
     6301 accggctgcg taaatctgat ggggttgaat ctgctgcagc aagtctttaa taatgtcgat
     6361 atccttttgc gataaatcgt ttttctttac tgttcccgtt tcataaaagg gaagattaag
     6421 aaaatgaatg ttttgagagc ttaaacccaa atatctacaa gctgcctttg cttctccccg
     6481 cctgattaat gcttttactt cgagaatttc tggtgtatcc atatcatttt cctttttttc
     6541 gtaaaggaga aaattcttca ttgcttcata tttggcctga atcacatcat tattgctgtc
     6601 gaataaatct ttaaatcctt tcacaaaaga aaggaagcga atgacttcat gatcggcaac
     6661 tgcgatattt ccggatgttt gataagctac atgcacttta tgattttggt ctattaacct
     6721 tttgaaagtt ccacccattg aaataacatc atcatcggga tgcgggctaa aaatgagaac
     6781 acgtttggga taaggaagtg ccctttcggg acgattagaa tcatcagcat ttggttttcc
     6841 accgggccat cctgtaatgg tgtgctgtaa atcgttaaaa attcggatat ttacattgta
     6901 ggcagagcca taaatagcca ataattcact taatccattg tcgttgtagt ccttattagt
     6961 caatttcaga atcggttttt tcaacttcat gcaaagccaa acaatggcac gacgtatcag
     7021 tttgctgtcc cactcacagg atgttaccag ccaaggcatg ctgatgcgcg tcaattcact
     7081 ggcagcatga agatcaataa ctatttttac attggaatga agttgaagat aggatgcagg
     7141 aatggtttcg gacatggggc cttctactgc attttttacg accgtagatt tatgttcgcc
     7201 ccatgcaacc aaaacaattc tttttgcttt aagaatagtt gaaattccca aagtaatggc
     7261 agaaacagga accatttcgg cattgctgaa aaattcagca gcatcttccc ttgaaagcgt
     7321 atcgagcaaa ataaggcggg tttgagagtt agtcagtgaa ccgggctcat tgaaaccaat
     7381 attgcctgag cgcccgattc ctaaaagctg taaatcgata ccgcccaaag cagcaatttt
     7441 ttgctcatat tctgcacaag cttgcatgat ttgatgtttg tccattgtgc catccggata
     7501 aaaaatattt tctgccggaa tatcaacatg atccaacagc atgtttttta attgtccata
     7561 acttcctttt aatttgcccg tgagcggata gtattcaaac aataaaaaaa caacaacatt
     7621 attaaaactc aagttttcct ttttgtgcaa tcgaaccagt tcagcataaa ttcccaccat
     7681 cgatttaccc gaagtaagac ctaaaacaca cttctcatgg gctgcctgtt ttgttttaat
     7741 taaggctgct atttcttctg ccacttttgc cgagccttct ttttctgtgt caaaaatttc
     7801 ggtcggtatt ttttcaagac gggtgaccct cgaaagttca aagacatcat cgggttgata
     7861 atatcgtcga ggaatacgcg tcatcgtaat ttttgagctt aaatttgttc tcataagtac
     7921 cttgataatg atgaatttgg tttcaaaagt aatacaattt caattaaaat gcaaacgttt
     7981 gcgttaaatt ttgttttttt cttgattttc tttggcaggg tgaattcttt tgctttaatt
     8041 tgtgcagaaa attaaattca acttaccgaa caatgaatat tatagctgac agcggatcga
     8101 ccaaaaccca ctggtgtgtt atagaaggtg aaaataccgt aaaggaattt tacacaaaag
     8161 gcatcaatcc attttatcaa accaaagaag aaatccttgc cgagctgaaa gaaaatcttt
     8221 acccacgaat catgcaaagt acgattgatg gagttcattt ttacggagca ggttgcgcct
     8281 ttgccgaaca aaaaaagatg ttaagcgaaa tttttagaga tattttggga aaagagcttg
     8341 tcgtaaccat agaaagtgat ttactcggag ccgctcgctc attgtttgga aaggaaagcg
     8401 gaattgcctg tattcttgga accggctcaa attcatgtta ttacaacggc agaggaatag
     8461 aaaaaaacgt tccgccattg ggatttattc tgggagatga aggcagtgga gctattcttg
     8521 ggaaaaaact gacatctgat gctttaaaaa atcaactacc gcccgatttg gtgtcaaaat
     8581 tcatgaaaga atatgatttg acattggaaa aggttctcga ccatgtgtac aaaaagcctt
     8641 tcccgaatcg ctatttggct caatttgtgc cttttataaa ggaaaatatt caatatccgc
     8701 ctttatataa tattgtatat agcagcttta acgatttttt taacagaaat atttgccaat
     8761 acccacatag tgaaaaaaca cccttaggat ttgtcggatc agtggctttt cactttgctg
     8821 acattttgca aaccattgca cgggaaagac atttcagaat aattgatata gcgcaaaatc
     8881 caatgaaggg attgattaaa tatcattcgc aaaaaaacaa ataattattt gaaaatatgg
     8941 catttgaaaa aattaccgaa caaaattcgc tgcacaacga tttggacaaa aaagacattc
     9001 gccaattgct cgttgaaatg aatgaagaag acaaaaaagt accatttgca gtagaaagag
     9061 ccattccaca aattgagcaa ctgattgaaa aactggtaga acgaatgaaa ataggtggac
     9121 gtttattcta tatcggagcg ggcaccagcg gacgtttagg tgtattggac gcttctgaaa
     9181 ttccacccac ttttggcatg cctccacatc tggtaatagg cgtgattgca ggtggtgata
     9241 cggctttacg caatgcggtt gaatttgctg aagacgatat ggaacaaggt tgggaagatt
     9301 tgaaaaaaca caatatttct tccatcgata ccgtagttgg catcaccgct tccggaacgg
     9361 caccctatgt gatgggagct ttgagaaaag caaaacaaca tggaattttg acagcttcca
     9421 ttgcctgcaa tcccgattcg cccgtatctc acgaagccga cataaaaata gaactcattg
     9481 tcgggccgga atttgtaacg ggcagtacac gtttgaaatc gggaactgcc caaaagttga
     9541 cactcaacat gatttccacc gttaccatga taaaactcgg aagagtgaaa ggcaataaaa
     9601 tggtcaacat gcaactaacc aattcaaagt tgattgatcg cggcacaaga attattgtag
     9661 aagaactgaa tatcgactac gaacaagccc gaaatatact gttgattcat ggctcagtga
     9721 aaaaagccat agatgcctac aaaattcaaa ataaaattta acattaaaat tttgttaaat
     9781 agaaaacaaa cgtttgcagt aaaagatttt ttttgtttat ttgcaatcac aaatgtttat
     9841 taaaaacatt attttcctaa taaaaaaggc tacatcaaac aacgcaattc aaacaattaa
     9901 ttattttagt acctatgaaa aaaattcttc tattatttgc tgttcttttt ctttcccttc
     9961 aagtgtcttt ggcacagaag gtaacagtta caggtacggt aacatctaag gaagatggac
    10021 ttcctatcat tggcgcttca gttctggaaa aaggaacgac aaatgggacc attacaaatt
    10081 ataatggggt ttatacaatt tcagttccta taggagctac tttggtattt agttatgttg
    10141 ggatggataa agtagaacgc aaagttactg gagagggcaa aatagatgta ataatgaatc
    10201 ccacagcaat tgccattgat gaagtaatcg ttactgctat gggtgtgaaa gcagagaaaa
    10261 agaaattaaa ttttgcagta cagtcggtag atgctgaagc tattacagac ggacgttctc
    10321 ctaattttgt caattcattg caaggaaaaa tttcaggagt tagtgtaacc agttccggag
    10381 gttcccccaa tgccggttca caaattttat tgagaggaat ttcttccatc aacccaagcc
    10441 aaaacaatga gcctttattt attctggatg ggatggttct ttccggtgga ggttctgcag
    10501 cagcagacat taatcccagt gatattgaaa atgtaaccgt tttaaaagga gcggctgctt
    10561 cagcattata tggacaaaat gcagctaatg gagcaataat gattacaaca aaaacagcaa
    10621 aagcagggaa aatgtcgctt tcagccaatg ctagcataca atcagatacg cctacgaggc
    10681 tactggagtt gcaatcaatt tatggaccag gtgctttggg attctatcga cctcaggaaa
    10741 gtggaggata tggtggttgg ggtccactat tagacaaaga tgaacctgtt tataacaatg
    10801 taaagaatta ttttcaaagc ggattatatc aaaaatacga tttcagtata aatggtggaa
    10861 gtgataaatt tcagtcttat gcatctgtaa actattcacg acatgatggt atagtaccga
    10921 aagattatct tgacaaaatg ggtttgctgt tgaaaggaaa ttacgaaatc aacaataaaa
    10981 tttccgccaa tttcatgacc aatattgtaa agaacagtta tcgaggcgca ggaagcatta
    11041 gtgccattta ttcttggcca atcaatgatg atattaccaa ttataaaaat tctgacggaa
    11101 gcatccgata tcgatatatc agtattccca aaaaagaaaa ttcacccatt agtcctttgt
    11161 ggagcaggta tatggattac ggattaaatt cttctacccg taatttatta cagggttcat
    11221 taatttacaa gccactcaaa ggcctggaaa ttacgggtcg attaagtctc gatcaaaaca
    11281 attacatgta tgacggatat c
//
LOCUS       DAOSCH010000067        11232 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3859_length_11232_cov_5.924756, whole genome shotgun sequence.
ACCESSION   DAOSCH010000067 DAOSCH010000000
VERSION     DAOSCH010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11232
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3859_length_11232_cov_5.924756"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..2154)
                     /locus_tag="PLZ54_06115"
     CDS             complement(106..2154)
                     /locus_tag="PLZ54_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOJ66536.1"
                     /translation="MKLKVNKKKFETVLKLLLAFALVIIIVELYPHESTFNYQFEEGK
                     PWSYELLTAPFDFPIYKSNEQIEKEKKEILKNFTPYFNLDTTVMHIQVNKFKIDSSRS
                     GNISPDLTTFIEEKLKSIYSKGIISNDDYNNLQKWNVKKIYRILPGRISQLATLNEIY
                     TPKTAYEEIVKSNQENIKELDINNYLVENLRYDSITSETAKNELLKNLSLTVGMVQSG
                     ERIIDKGEIVSPEAYAILRSLQIENENRKSAFEQTAIVLTGKILLVIGLITLLILYIY
                     LFRPRILDNFNNLLFLSLLIILIVGLTSLTIRYSSLSYYIVPFALLPIIVRVFYDSRT
                     ALFVHIIVIFIVSFMTDNPFQFALLQITAGMVAVSSLKDMTQRSQLAQTALYIFLSYT
                     VVYLSLELMTEGNISKIDWYPIISFAVSSLFLLFAYLLIYIFEKMFGLLSAVTLVELT
                     NVNSDLLMKFAEVAPGTFQHSLQVSNLATEAAKKINANSLLVRTGALYHDIGKMKNPE
                     FFTENQIGGKNPLLSMQYEEAAKLIIEHVANGVEIARKNHLPNQIINFITTHHGTSKT
                     KYFYVSFINDNPGIEPPEDVFSYPGPLPNTKETAILMMADAVEARSRTLSEYTEESID
                     QMVESIINTQIAEGQLKDAPISFRDVETVKKVLKEKIKSIFHSRITYPEINKNLSTNV
                     "
     gene            complement(2998..4545)
                     /gene="dnaB"
                     /locus_tag="PLZ54_06120"
     CDS             complement(2998..4545)
                     /gene="dnaB"
                     /locus_tag="PLZ54_06120"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOJ66537.1"
                     /translation="MAEKKTYNKKQNNAPTVIAPNEMGKLPPQALELEEAVLGAIMIE
                     KDAYFLVSEILKPVCFYKPTHQKIYEAIMTLALHQEPIDMHTVTEQLRRDGTLEEIGG
                     PYYITLLTAKVSSAAHLEYHAHIILQKFLARELIRISSEIQTKAFDDKTDVEDLIEES
                     EEMLFEIAQRNLEKEVIQIKPVIDEAIQRIQAAANKQGTSGVPSGFHSLDKITSGWQK
                     SDLIIIAARPAMGKTAFVLSMAKNMAVDYNVPVGVFSLEMSSVQLANRLIMNVCEIDG
                     VKIKNGDLSPYEWDKLDKNIKDLIDAPVFIDDTPSLSISELRSKARRLVKEHKVQCIF
                     IDYLQLMNASEMRFSSREQEVSTISRSLKSLAKELDIPIIALSQLNRNVEGRSGVDGK
                     RPQLSDLRESGAIEQDADVVCFIHRPEYYNLTTDKDGNSMIGIAEIIVAKHRNGATDT
                     IHLKFKKEFAKFLNLDDIDSDDIFNENYQVISSKMNVPEGNFYENTSGSPALDNLLKN
                     NNSEVPF"
     gene            4824..6557
                     /gene="lysS"
                     /locus_tag="PLZ54_06125"
     CDS             4824..6557
                     /gene="lysS"
                     /locus_tag="PLZ54_06125"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOJ66538.1"
                     /translation="MNIELSDQEQYRRQSLTELRNLGIEPYPAALYPTNAFSTDIKSN
                     FDENNPQRMEVCVAGRIMSRRIMGKASFVELQDAKGRIQIYITRDDINTEAQPEMYNT
                     VFKKLLDIGDFIGIKGYVFRTQTGEISVHAKELTVLSKSLRPLPIVKYKDGIAYDAFD
                     DPEQRYRQRYVDLVVNEGVKDIFLKRTKVYQSMREFFNTRGYVEVETPILQPIPGGAA
                     ARPFITHHNALDMPLYLRIADELYLKRLIVGGFEGVYEFSKNFRNEGMDRTHNPEFTA
                     MEIYVAYKDYFWMMDFTEQMIEKICLDVNGTTEVRVGDKTISLKTPYKRISMIDAIKE
                     NTGIDIEGMDENDLRKVCEDLQIDVDETMGKGKLIDKLFGEKCEANYVQPTFITDYPL
                     EMSPLCKRHRNNPELTERFELLVNGKELANAYSELNDPIDQLERFQEQLRLSEKGDDE
                     AMFIDMDFVRALEYGMPPTSGMGIGMDRLVMLLTGQQAIQEVLLFPLMKPEKVQKVDT
                     KEKFMELDIPEAWAEVLQKMGIKQVDQLKDLNPNKLHQDICGFNKKNKLALTNPSKEE
                     VSQWIDNAAKK"
     gene            6934..7899
                     /locus_tag="PLZ54_06130"
     CDS             6934..7899
                     /locus_tag="PLZ54_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOJ66539.1"
                     /translation="MFEIVFELSSLADTAVFYGINNSNMQLIKNLHPNLRIVARGNAI
                     KIIGNQQDADKLIVNLQKLEKFSIDNNQLTEKNIIELIKGENPKDISFQNLIMHGVGG
                     KSIFARTKNQQRLVEEFEKNDLLFAIGPAGSGKTYTAIALAVRALKNKQIKKIILSRP
                     AVEAGEKLGFLPGDMKEKIDPYLQPLYDALQDMITPLKLKEYMENGTIQIAPLAFMRG
                     RTLSDAVVILDEAQNSTIMQIKMFLTRMGLNTKMIVTGDITQIDLPRDQKSGLIDALN
                     LLGNIKGIARIDFDVNDIVRHRLVQQIVQAYDNRAMNERQKNTKE"
     gene            7973..8926
                     /locus_tag="PLZ54_06135"
     CDS             7973..8926
                     /locus_tag="PLZ54_06135"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOJ66540.1"
                     /translation="MNTLVRTDFHFEGQTNVYHGKVRDVYFIQDDLLVMVATDRISAF
                     DVILPKGIPYKGQILNQIAAKFLDLTADIVLNWKLACPDPMVTVGLKCEGFPIEMIVR
                     GYLCGSAWRAYKNGVREICGVKLPDNMRENQRFETPIITPTTKAELGTHDEDISRQEI
                     LAKGLLTSEEYDLLEKYALALFERGTQVAAERGLILVDTKYEFGKRNGNIYLIDEIHT
                     PDSSRYFYAEGYEERFEKGEPQKQLSKEFVREWLMENGFQGKEGQKVPEMTDEIVNSI
                     SERYIELYEQITGESFQKTDTRNLAERIESNVMKYLQSLKK"
     gene            complement(9021..10574)
                     /locus_tag="PLZ54_06140"
     CDS             complement(9021..10574)
                     /locus_tag="PLZ54_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOJ66541.1"
                     /translation="MFKNHPKGLIAASLANMGERFGFYTMMAILSLFLMAKYGLDETK
                     AGLIYSVFYFSIYILAFVGGLIADKTRNYKRTILWGLILMSIGYFLVALPTPTPVPQN
                     RYGFFLGLTCFGLLTIALGNGFFKGNLQALVGQMYDNPKYEKMRDSGFSLFYMFINVG
                     AIFAPFAAIGVRNWWLHSHGFAYNPDLPALCHDQLNGIITPEAAQRMHELAVNAGYTG
                     SNMTSFADQYLNLFTTGFHYAFMVAIGAMFVSLIIFVTNKKSFPQPRNLSSTATVTLD
                     QNGKEMDAKEIKQRLYALLAVFAVVIFFWFSFHQNGLTLTFFAKDYTNLSKVSVNLGF
                     TTLQGAEIFQSINPFFVVFLTPLVMALFGYLRSKGKEPSTPRKIAIGMGIAALAYVVM
                     TLGSLGLPSKEEITLRGGLPDAERVTPLLLLGTYFILTVAELFISPLGISFVSKVAPQ
                     KYQGIMQGGWLAATALGNQLLFLGALMYKNIPIWMTWSVFVGACLISMFTMLAMLKWL
                     ERITGEKQG"
     gene            complement(10722..>11232)
                     /locus_tag="PLZ54_06145"
     CDS             complement(10722..>11232)
                     /locus_tag="PLZ54_06145"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOJ66542.1"
                     /translation="RIFLIGYMGAGKTTIGQQLATRLGYKFLDMDHVIEEKMQQSVSQ
                     IFSEKGEKEFRRLERECLDKISTEQNVVVATGGGAPCFYDNMERMNAYGLTVYLCLSP
                     EQLQERLELSHKNKRPLLAQREGEELLDFIRQNLSEREPFYRKATLVVSGTDEEMFQQ
                     ILSFLSSHS"
BASE COUNT         3613 a         2085 c         2119 g         3415 t
ORIGIN      
        1 gatggaaccc acatgtctga atattatatt cattcgtgtt tgtgtatgca atgcaggtgg
       61 gtttcatgtt gttattttta tccgatttat ttgctcagtt gcaatttaaa catttgttga
      121 taagtttttg tttatctccg gataagtaat tcgagaatga aagatacttt ttattttttc
      181 tttcagaacc tttttaacag tttcgacatc tctaaaacta atgggagcat cttttaattg
      241 tccttcggca atttgcgtat tgataatact ttccaccatt tgatctatgc tttcttctgt
      301 atattcgctc agagtacggg aacgggcttc cactgcatcc gccatcatca aaatggctgt
      361 ctccttagta tttgggagag gaccgggata tgaaaacaca tcttcagggg gttctatgcc
      421 gggattatca ttgataaagg aaacataaaa atattttgtt ttacttgtgc cgtgatgtgt
      481 agtaataaaa ttaataattt gattgggaag atgatttttc ctggcaattt ctactccatt
      541 ggcaacatgc tcaattatca gttttgcagc ttcctcgtac tgcatggaga gcaaaggatt
      601 ttttcctcct atttgatttt cggtaaaaaa ttcagggttt ttcatttttc ctatatcatg
      661 gtaaagagct cctgttctta caagtaaact attggcattg attttttttg cggcttcagt
      721 tgccaggtta gacacctgaa gtgaatgctg aaaagtaccg ggagcaactt cggcaaattt
      781 catcaacaaa tcgctattga cattggttag ttctaccaat gttacagccg aaagtagtcc
      841 aaacattttt tcgaatatat atatcagcaa atatgcaaac aaaaggaaaa gggaactgac
      901 agcaaaacta ataataggat accaatcgat tttggaaata tttccttctg tcatgagttc
      961 gagcgaaaga tatacaacgg tataacttaa aaaaatgtat agcgcagttt gtgccaattg
     1021 ggaacgctgt gtcatatcct ttaaactgga gacggcaacc atgccagcag ttatttgcaa
     1081 caaagcaaat tggaatggat tgtcggtcat aaaagacacg ataaaaataa cgataatatg
     1141 tacgaaaaga gccgttcgag aatcgtaaaa tacacgaact attatgggta ataaagcaaa
     1201 aggaacaata tagtagctta acgaagaata tcgaatagtc aatgaagtaa gccctacaat
     1261 taggatgatg agcaaggaca aaaacagtaa attattaaaa ttgtccaaaa ttcttggtcg
     1321 aaaaagataa atgtaaagaa tcaggagcgt aattaaaccg attaccaata aaattttgcc
     1381 tgttaaaact atagccgttt gctcaaatgc cgattttctg ttttcgtttt cgatttgtaa
     1441 agatcggagt atggcataag cttcgggaga aacgatttca cctttatcga taattcgttc
     1501 gcctgattgc accatgccaa cagtaagtga aagattttta agcaattcgt ttttggctgt
     1561 ttcggaagtg atgctgtcat agcgtaggtt ttctaccaga taattgttaa tatctaattc
     1621 cttgatattt tcctgattgc ttttaactat ttcttcatat gcagtttttg gcgtgtaaat
     1681 ttcatttaag gtagcaagtt gcgaaattct tcccggtaaa atacggtaga tttttttcac
     1741 attccatttt tgcaaattat tgtaatcgtc attcgaaata attcctttcg agtaaatcga
     1801 tttcaacttt tcttcaatga aagttgtcaa atccggggaa atattgcctg acctgctgct
     1861 gtcgatttta aatttattta cctgaatatg cattacagtg gtatccagat tgaagtaagg
     1921 tgtaaaattc ttgagaattt cctttttctc tttttcaatt tgctcattgc ttttgtatat
     1981 cggaaaatca aaaggtgcag tcaaaagttc atacgaccaa ggtttacctt cttcaaactg
     2041 ataattgaaa gtgctttcat gcggataaag ttctacaatt attattacca aagcgaaagc
     2101 aagcaaaagt tttaaaacgg tttcaaattt ctttttgttg actttcagtt tcatgacttt
     2161 ttgttttttg atttactact gtaaaaatct ttatttctct aatcagcaaa aatttgtgga
     2221 atgttcagat tttgcttgtt aataattcct gttttttcat cagaatataa ttaatttttt
     2281 aaaggtctga tggaatccaa atgtcaatat tatattcatt cgtgtttgtg tatgcaatac
     2341 atgtaagttt catttaaaat tttgttttgt aaagatttcg acgccgtaaa aaagctaaaa
     2401 attaaaaaaa cacgccttat gcttatcagt tttatttact tgtgaatcaa aaattaagaa
     2461 tatgagccac tatagaaaaa agaatggaaa taccaaaaga attgggttga tagcattatg
     2521 ttgcatgtaa aatatagtta ttctgatggt aaagatacaa caaaaaatta ttttgaaggc
     2581 cgaatttgaa tcaacgataa aatgttttca tgccaccaat ttgatgcgat gtttaatctg
     2641 tgggtaagag atatataatt ttacattgtt gtccggtaaa catttataaa aattagtgaa
     2701 aaccaatata gaaaatgtct tactataata tcgcccctaa tggggcttag attttgtggg
     2761 ttgaattttt ctaccataat ttcgctgcaa tgcagctttc tcttaagtcc catcgggacg
     2821 atattatggt agtcaataag ttacaaatat cattagagct ccgtaggagc gacactattt
     2881 gctcctttat ttttatcgga caacattaat aattttaaaa attcttatga tgaatggagc
     2941 ccgctttaat ttatattgaa aagcagatgt ttttcaagat gtgattggta atttctatca
     3001 gaaaggaacc tcgctattat tatttttcag caaattatcc agtgcaggcg agccagaagt
     3061 attttcataa aaattaccct caggcacatt cattttggaa gaaataacct gatagttttc
     3121 gttgaaaata tcatcggagt cgatgtcatc aagatttaaa aacttggcaa attctttttt
     3181 gaattttaaa tggatggtat cggtagcacc gtttcggtgt tttgcgacaa taatttcggc
     3241 tatgccaatc atggaattgc catctttgtc ggtagtcaag ttgtagtatt cgggacgatg
     3301 aataaagcag accacatcgg catcctgttc tatggcacct gattcacgca aatcggacaa
     3361 ttgcggacgc ttgccatcaa ccccgcttct tccttccaca tttcgattca gctgcgaaag
     3421 cgctataatg ggaatatcca attctttggc aaggcttttc aacgagcggg aaatggtact
     3481 tacttcctgt tcgcggcttg aaaatcgcat ttcactggca ttcatcaatt gcagataatc
     3541 gataaaaata cactgtactt tgtgctcctt gaccaacctt cgtgccttgc tcctcaattc
     3601 ggaaatcgaa agacttggcg tatcgtcaat aaaaacaggt gcatcaatca aatcttttat
     3661 gtttttatcg agtttatccc attcataagg actcaaatcg ccatttttta tttttacacc
     3721 atcaatttcg caaacattca ttatcaaacg gtttgctaat tgtacactgg acatttcgag
     3781 cgaaaaaacg ccaaccggaa cattgtagtc aactgccata tttttagcca tagacagaac
     3841 aaaagcagtt ttacccattg caggacgagc agcaataata attaaatcgg acttttgcca
     3901 gccggaagta attttatcca aactgtgaaa accacttggt acaccacttg ttccctgttt
     3961 attggcagca gcttgtatgc gctgaatggc ttcatcaatc accggtttga tctgaataac
     4021 ctccttttcg agattacgct gcgcaatttc aaacaacatt tcttccgact cttctatcaa
     4081 atcctctaca tcggttttgt cgtcgaaagc tttggtctga atttcactgg aaatacgaat
     4141 aagttcccga gctaaaaatt tctgcaggat aatatgcgca tgatattcca aatgtgcagc
     4201 cgaagaaact tttgcagtca acaacgtaat ataatatggg cctccaattt cttcgagcgt
     4261 accatcccga cgcaactgct ccgttacagt gtgcatgtcg ataggttcct gatgcaaagc
     4321 cagcgtcata atggcttcat aaattttctg atgggtgggt ttgtaaaaac acaccggttt
     4381 caaaatttcc gaaaccagaa aataggcatc tttttcaatc ataatcgctc ccaaaacagc
     4441 ctcctccagc tcaagcgcct gaggtggaag ttttcccatt tcatttggag caatcactgt
     4501 gggtgcatta ttttgttttt tgttgtatgt ttttttttcg gccattggta ttttctatgt
     4561 gtttatgtaa agtttgaatt aacttttcaa aaatacaatt attttccaaa tttgagagaa
     4621 ttttttgtca acagctgttg ataagtttta taggaaaaca ccaaataatc ctgaaaaata
     4681 tgaccttaag ctgtaaaaat ttttatcgtt tcatctgttt tttccataaa aagttcaaac
     4741 accaaagatt tgacaggcct cacagtgtga gaattgaaaa ttatcatgta actttgcatg
     4801 aaaattgaaa ttacttcgta cacatgaata tagaacttag cgatcaggaa caataccgaa
     4861 gacaaagtct cacggaatta cgaaatttag gcatagagcc ctatcctgct gcactttatc
     4921 caaccaatgc tttttctaca gatataaaat cgaattttga cgaaaacaat ccccagcgca
     4981 tggaagtttg cgtagccggc agaatcatga gtcggcgcat catgggaaaa gcttcgtttg
     5041 tagaactgca ggatgcaaaa ggacgtattc agatttatat tacacgagat gatattaata
     5101 ccgaagcaca acccgaaatg tacaacacag ttttcaaaaa actgttggat attggcgact
     5161 ttatcggtat caaaggttat gttttccgta cccaaacagg tgaaatcagt gtccacgcaa
     5221 aagaattgac ggtactttcc aaatcacttc gtccactgcc tatagtaaaa tacaaggatg
     5281 gcatagccta cgatgctttt gacgatcccg aacagcgcta caggcagcgt tatgtagatt
     5341 tagtagtgaa tgaaggcgtg aaagatattt ttcttaaacg aacaaaagtt taccaatcca
     5401 tgcgcgaatt ttttaatact cgcggttatg tggaagtgga aactccaatt ttgcaaccta
     5461 tccccggtgg cgctgctgct cgacctttca tcacccacca caatgctttg gacatgccgc
     5521 tgtatctgag aattgcggat gaactttatt tgaaaagatt aattgtgggc ggttttgaag
     5581 gtgtttatga attttctaaa aatttcagaa acgaaggtat ggatcgtacc cacaatccgg
     5641 aatttactgc tatggaaatt tatgtggcat ataaggatta tttttggatg atggacttta
     5701 cggaacaaat gattgaaaaa atttgtctgg atgtaaatgg aacaaccgaa gttcgggtgg
     5761 gtgataaaac aataagcctg aaaacgcctt acaagcgtat ttccatgatt gatgccatta
     5821 aggaaaatac aggtatcgat attgaaggaa tggatgaaaa tgatttgaga aaagtatgtg
     5881 aagatttgca aatagatgtg gacgaaacga tgggaaaagg aaagcttatc gacaaacttt
     5941 ttggagaaaa atgcgaagcc aattatgttc aaccaacttt tattacggat tatcctctgg
     6001 aaatgagtcc gttgtgcaaa cgtcatcgca ataatcccga acttacggaa cgttttgaat
     6061 tgttggtgaa cgggaaagaa ctggccaacg cctattccga actcaacgat ccgattgacc
     6121 agttggaaag atttcaggaa cagcttcgac taagtgagaa aggagatgac gaagccatgt
     6181 ttattgacat ggattttgtc cgcgcattgg aatatggaat gcctcctact tcaggaatgg
     6241 gtatcggtat ggacagacta gtgatgctgc ttaccggtca gcaagccatt caggaagttt
     6301 tgctttttcc gctgatgaaa cctgaaaaag ttcaaaaagt cgataccaaa gaaaaattca
     6361 tggaattgga tattcctgag gcatgggctg aagtacttca aaaaatggga ataaaacagg
     6421 ttgaccaatt gaaagatttg aatcccaata aattgcatca ggacatttgc ggattcaaca
     6481 aaaaaaataa acttgcattg accaatcctt ccaaagaaga agtcagccaa tggattgata
     6541 atgcagcgaa aaaataatca ttacgaatcg ttttacagtc tgatattgtt atctttagtt
     6601 tacttgtatt ttagagttta attctttgta ttgagttggt ttttaaatta aaaaaaatta
     6661 atgttgtccg ataaaaataa aggagcaaat agtgtcgctc ctacggagct ctaatgatat
     6721 ttgtaactta ttgactacca taatatcgtc ccgatgggac ttaagagaaa gctgcattgc
     6781 agcgaaatta tggtagaaaa attcaaccca cataatctaa gccccattag gggcgatatt
     6841 atagtaagac attttctata atggttttca ctaattttta taaatgttta ccggacaaca
     6901 atgaaaaaat cttttttatg atgctcaaat cttatgtttg aaattgtttt tgaactttct
     6961 tctttggccg atactgcggt tttttatgga ataaacaatt ccaatatgca gttgataaaa
     7021 aatttacatc ccaatttacg gattgtggcg agaggaaatg ctattaaaat aattggaaat
     7081 cagcaggatg cggacaaact aattgttaat ttgcagaagc ttgaaaaatt cagcatcgac
     7141 aacaatcaac ttactgaaaa aaatatcatc gaattaataa agggtgaaaa tccgaaggat
     7201 atttcgtttc aaaacctgat tatgcacggt gttggaggaa aatcaatttt tgcacgcacc
     7261 aaaaatcagc aacgtctggt agaagaattt gaaaaaaatg atttgctctt tgccatcggg
     7321 cctgccggtt cgggaaaaac ctatacggca attgctttgg ccgttcgggc attgaaaaac
     7381 aaacaaatca aaaaaattat attgagtcgg ccggctgtag aggccggcga aaagttgggt
     7441 tttcttccgg gcgatatgaa agagaaaatc gacccctatc tgcaaccgct ttacgatgct
     7501 ttgcaggata tgattacccc gctcaagctg aaagagtaca tggaaaacgg taccatacaa
     7561 attgcaccgc ttgctttcat gcgtggacga accctgagcg atgccgtcgt aattctcgac
     7621 gaagcccaaa attctaccat catgcagata aaaatgtttc tcacccgcat gggactgaat
     7681 accaaaatga tagttaccgg cgatattacc cagattgatt tgcctcgcga ccagaaatcg
     7741 ggtttgattg atgcactgaa tttgcttggc aacatcaaag gcattgcccg catcgatttt
     7801 gatgtcaacg atattgttcg acatcggctg gttcagcaaa ttgttcaggc ttacgacaat
     7861 cgggcaatga acgaaagaca aaaaaatact aaagaataaa taaccgaacg aatcatttgt
     7921 gattttttcc tttttttctt taaaactata tcaaccctca aaactaaaaa ttatgaatac
     7981 ccttgttcgc accgattttc atttcgaagg acagaccaat gtttatcatg gtaaagttcg
     8041 cgatgtttac tttatacagg atgacctgct ggtaatggtt gctaccgatc gtatttcggc
     8101 tttcgacgtt attttgccta aaggaattcc gtataaggga caaattttga atcaaattgc
     8161 agccaagttt ctcgacctca cagccgacat tgttcttaat tggaaactcg cctgccctga
     8221 tccaatggta accgtgggat taaaatgtga aggttttccc atcgaaatga ttgtgagagg
     8281 ttatttgtgc ggcagcgctt ggcgtgctta taaaaatggc gttcgagaaa tttgcggggt
     8341 taaacttcct gacaacatgc gcgaaaatca gcgttttgaa acacctatta ttacgcccac
     8401 tacaaaagcc gaattgggaa cgcacgacga agatatttcc agacaggaaa ttcttgcaaa
     8461 aggattgttg acttctgaag aatacgattt gttggaaaaa tatgcactgg cactttttga
     8521 acgtggcacc caagtggctg ccgaaagagg attgattttg gtagatacca aatacgaatt
     8581 tggaaagaga aacggaaaca tttatctgat tgatgaaatt catacgccgg attcttcccg
     8641 atatttttat gcagaaggtt atgaggaacg ttttgaaaag ggtgagccac aaaaacagct
     8701 atcgaaggaa tttgttcgtg aatggttgat ggaaaacggt tttcagggaa aagaaggaca
     8761 gaaagttcct gaaatgactg atgaaattgt caacagtatc agtgaaagat atatcgaact
     8821 ctatgaacaa attacgggtg agtctttcca gaaaaccgat acacgaaatc ttgccgaacg
     8881 catcgaaagc aatgtaatga aatatctgca aagcttaaaa aaataaacct acgcatcggt
     8941 aggccgataa taaagaatcc actctttaat tgaaaccttt cgggctttaa agagtggatt
     9001 ttttattgga aaattttcat ttatccttgt ttttcaccgg ttatccgttc gagccatttc
     9061 aacatggcca gcatggtgaa catggaaata aggcaagccc cgacaaaaac gctccaagtc
     9121 atccaaatcg gaatgttttt gtacatcagt gcaccaagaa aaagcaactg atttcccagt
     9181 gccgtagcag ccagccaacc accttgcatg attccctgat atttttgtgg tgccactttt
     9241 gaaacaaaag aaattcccag cggactgata aacagctcgg caaccgttaa aatgaaatac
     9301 gttcccaaca gcagtaatgg cgtaacgcgt tcagcatcgg gcaatcctcc tcttaaagta
     9361 atttcttctt tcgaaggcaa gcccagcgaa cccagcgtca tcacaacata agccaaagca
     9421 gctattccca taccaatcgc aatttttcgt ggcgttgaag gttcttttcc cttgctgcgc
     9481 aagtaaccaa acaaagccat aaccaacggg gtgagaaata ccacaaaaaa cgggttgatg
     9541 gattgaaaaa tttctgctcc ctgcagcgta gtaaatccaa gattcacgga aactttgctc
     9601 aggttggtat aatctttggc aaaaaaagtc agggtcaagc cgttttggtg gaaagagaac
     9661 caaaagaaaa ttaccactgc aaatacggcc aacaaggcat acaaacgttg tttgatttct
     9721 ttggcatcca tctctttacc gttttggtcc aaagttactg ttgcggttga agataaattg
     9781 cgaggttgag gaaaggattt tttgtttgtt acaaaaatta tcaacgaaac aaacatggcg
     9841 ccaatggcaa ccataaaagc ataatgaaaa ccggtggtaa acaaattcag atactgatct
     9901 gcaaaagaag tcatatttga acctgtgtaa ccggcattaa cagccaattc atgcatccgt
     9961 tgagctgctt cgggcgtaat gatgccattc aactggtcat gacacaaagc aggaagatca
    10021 ggattataag caaagccatg agaatgtaac caccagtttc tcacaccaat agcagcaaaa
    10081 ggagcaaata tggctcccac attaatgaac atgtaaaaca gtgaaaaacc cgaatcacgc
    10141 attttttcgt acttcggatt atcatacatt tgccctacca atgcctgcaa attcccttta
    10201 aaaaatccat tccctaaagc tatggtcaac aatccgaaac aggttaatcc gagaaaaaac
    10261 ccgtatctgt tttgtggaac aggagtgggt gtaggaagcg ctactaagaa gtaaccaatt
    10321 gacattaata ttagccccca caagatggtt cttttataat ttcttgtttt atcggctatc
    10381 aatcctccaa caaatgccaa aatataaatt gaaaaataaa aaacagaata aattaagcct
    10441 gcttttgttt catccaatcc gtattttgcc atcaaaaaca acgacaaaat ggccatcatg
    10501 gtataaaatc caaaacgctc ccccatattg gcaagcgaag cagctataag tcctttggga
    10561 tgatttttaa acataaactt gatctcattt tagtgaaact atcagaagaa taatttataa
    10621 aaatgcaaaa atgaaaaatt tatttattcc aatccatatc ctgaccaaaa aaattaaatt
    10681 gttttaacta attatttcat ctgacaacaa ttcaatcaaa attaagaatg gcttgacaaa
    10741 aacgacagaa tttgttgaaa catttcttca tccgtaccac tgacaaccaa cgttgcctta
    10801 cgataaaaag gttcccgttc cgacaggttt tgtcgaataa aatccaataa ttcttcacct
    10861 tctcgctgag ccagcaaggg acgtttattc ttgtgtgaaa gttcgagccg ttcctgtaat
    10921 tgttcagggc ttaaacataa ataaacggtt aagccataag cattcattcg ttccatgtta
    10981 tcgtaaaagc agggtgctcc accaccggtg gcaaccacca cattttgttc ggtggatatt
    11041 ttatctaaac attctctctc caatctccga aattcttttt ctcctttttc cgagaaaatt
    11101 tgcgaaacgg actgttgcat tttttcttca atcacatgat ccatgtccaa aaacttataa
    11161 cccaatcggg tagccagttg ctgtccgata gttgtctttc cggctcccat atagccaatt
    11221 aaaaatattc ga
//
LOCUS       DAOSCH010000068        11158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3891_length_11158_cov_7.150410, whole genome shotgun sequence.
ACCESSION   DAOSCH010000068 DAOSCH010000000
VERSION     DAOSCH010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3891_length_11158_cov_7.150410"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="PLZ54_06150"
     CDS             <1..511
                     /locus_tag="PLZ54_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66543.1"
                     /translation="TPDGNIKVLYKIQIPLYLKPGSYYEFKDTIPDAGEDINSALEDV
                     DVNSGSIFLTVTNGLPVKGKLTLVLKDSLGRDISTTFKKEYDIDAPQVDAEGFVVESS
                     ITPQKIEIALNNDQLNDFKRSKDIVYKFRIDSKDANSFIHFRKSDTFKVKLGLFVNGK
                     VSYDSNNND"
     gene            527..2986
                     /locus_tag="PLZ54_06155"
     CDS             527..2986
                     /locus_tag="PLZ54_06155"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOJ66544.1"
                     /translation="MKKIIISILTGICCLTCIFSTSAQQVNSLYFMENSPIRSYLNPA
                     FQPTGSFYLGLPVLGYTYLSLGNNSLTLKDLVYNKNGETIWFLNPEGDVDKFYKSLRS
                     STLLNTNEQLNLLNFGFRAGSAYWTFGITEKVEGNLALPKDMFKLLLYGTPEQENNYF
                     NFKDLNANVMAYTEFALGYSKIINDQWTVGGKLKFLAGNAIVTSSNDYFNLNAGIEQW
                     NMKGTGSLNIAVPGEVTIGDKFESIDYTEPEDMQSWLKPSGMGLGFDLGFTYKPLENV
                     TLSASLIDIGTIKWNKNMKKVAYNMDYTFDGLAKIDNLDSVDMDAFLDTLSNAFKNSF
                     TTEEKATAFSTATSPKFNLGAEYAFANNKLSLGLLSTTTKVKSNMYQELTAAFNMRPS
                     NWFNLSLAYSLLNGRMSNIGAALGIRTGFFYWNLSSDYVSLDNAPLLLSDIDSSLPST
                     KIPIPYNSKGLNLALTVNFVFGNKVDKDHDGVVDKKDKCPDTPKAARKKVDANGCPLD
                     TDQDSIPDYLDQCPDTPLAARGFVDSKGCLLDTDNDSIPDYLDKCPDTPLGVAVDSLG
                     CPLDTDKDSVPDYLDRCPDTPLEAHGFIDENGCLLDTDQDGVPDYLDLCPNTPLEAIN
                     FIDKNGCPMDSDSDGVPDYLDQSPNTPLEARGLVDEKGAPRDTDGDGVYDFKDDCPKI
                     PGDSTNHGCPEVKKEVRTLFQRALKGIQFDTGKDIIKPSSYPILDQIAQVLKENPTYL
                     VEIQGHTDNVGNPQKNMELSEKRAIAVKKYLVSKGVEENRITAKGYGDTMPVASNKTA
                     AGRALNRRVEFIVTFEEVKFE"
     gene            complement(3079..3801)
                     /locus_tag="PLZ54_06160"
     CDS             complement(3079..3801)
                     /locus_tag="PLZ54_06160"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOJ66545.1"
                     /translation="MDVQLFIPCYIDQLYPQTGFNVIKILEKVGCRVHYNSNQTCCGQ
                     PAFNSGYWKETAYLAEKFLCDFNSDMPIVSPSGSCTGFILHHYNKLLANNPELLQKFK
                     EIKSQLYELTDFLVNILKTEDLGAVFPHKITFHDACSALREYGLKDEPRKLLKQVEGL
                     QLVEMDENDTCCGFGGTFAVKNHAISTSMTARKVANSLRTEAEFIVSTEASCLMNINT
                     YCKKHNLSICGIHLADVLASGY"
     gene            complement(3833..4879)
                     /locus_tag="PLZ54_06165"
     CDS             complement(3833..4879)
                     /locus_tag="PLZ54_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOJ66546.1"
                     /translation="MKSSITSGKKRVFLATTICFLFAVGLYCQEKRNFKIVSYNVENY
                     FDCVDDSLTDDQEYLPNGIRAWNTTKYKKKQANIGKVICAIGGWESPALVGLCEVESE
                     KCLVDLTRYAGLKNLKYKFLHHESPDARGVDVALLYQPYVFKPFHDEAIRINFPQFPE
                     IKTRDILFASGIIPSGDTLHVFVCHFPSRLGGELESEERRIFVASVLRSKVDSLFSSN
                     NKANIVIMGDFNDYPPNRSLSETLKALPPTEPFLQKNLYNLMFPMHLKGKGSHKHNGE
                     WGTLDQIIVSGNLLTPTNSVYTLPSDAHIFEADFLLEDDRTFLGKQPFRTYVGMKYQG
                     GFSDHLPVYVDLWY"
     gene            complement(4921..6399)
                     /locus_tag="PLZ54_06170"
     CDS             complement(4921..6399)
                     /locus_tag="PLZ54_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOJ66547.1"
                     /translation="MFKFKKTVFFFTKLIILWAIINFFSSCQEDFTTNPADTVLFSTD
                     TLTFDTTFTSIGSATSRIMVYNPNNKAIRISSIFLASGKSSAFKINVDGKNNENNQFA
                     DIEIGAKDSLYIFVQVNVDPLDNNLPLLIEDALIFLVNGNIKTITLQAIGQDAIIFRN
                     KLIANDTTLTAEKPYLVFGYLAVDSAKTLRLDPGLKIYFHNNANLIVYGNLMADGTFE
                     KPITMRGDRFDKIKFETPVLYNVVAGQWGGIYLLWNKGKHVLHNVNLNSGYVGIYFSN
                     QDKNQLPELEISNCRIHNFLLYGLVVQNGNVKVLNSEISNSSSFCVYLNGGQHTFIHC
                     TIANYFNNSSVQPILRDKEPAVMIMDLNRIAPMRTTFLNCAIAGSMENEFTLATRFPK
                     LYKGTFSHCYIRRPNALDLPQFSNIRWSEKNDTVFKNIYFDYKKGTYFNFEPDSVSPL
                     RGLADYNIAAENCPVDLNGINRLQDGAPDAGAYEWMPAKKDE"
     gene            6529..6798
                     /locus_tag="PLZ54_06175"
     CDS             6529..6798
                     /locus_tag="PLZ54_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66548.1"
                     /translation="MASRKELKKQINDLTGQLALEILFRGTVMQVLEPEKMYALLSKV
                     MIMNRQFISRVNASKRIKSSKEVKDFYQQFFTDWEKNVGEIIQEF"
     gene            complement(6838..7551)
                     /locus_tag="PLZ54_06180"
     CDS             complement(6838..7551)
                     /locus_tag="PLZ54_06180"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOJ66549.1"
                     /translation="MLKNNIFAFIFILTFCSVFFPKVKAQGNLPYVDDKLIHFGFSLG
                     LNTADFGITPRDTLINGSRYYTDVSALQPGFSVGVITNLRLARYWNLRFNPTLHFSER
                     DITYRKDTTSTTGSTTVNSIPLSFPVYLKYSAERKDNYRPYLLGGGGVYFDFGRNKEK
                     PILLKPMDYFVEFGVGCDLYFSFFKLAPELKFAIGFNNMLTPLEQRNAGGLTEEDKIY
                     STAISKLTNKMITLSFNFE"
     gene            complement(7674..8066)
                     /locus_tag="PLZ54_06185"
     CDS             complement(7674..8066)
                     /locus_tag="PLZ54_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66550.1"
                     /translation="MKKILFLLFLFSNSALIGQQIYNAAGKPIGKIIGQNYYDNNSKL
                     KGKVESVFVYDAAENLIGKIDGSYLYDGNGKQIGKVENNDFYDALGTKLATVSGNALY
                     DSYGRRIRKFDGLTLSQIIVYYYYFSNL"
     gene            8312..8833
                     /locus_tag="PLZ54_06190"
     CDS             8312..8833
                     /locus_tag="PLZ54_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HOJ66551.1"
                     /translation="MNRLDAVFFASDAITVARQLLGKTLVRKWEDGTISRYKITETEA
                     YFGEEDKACHACKGKTQRTQIMYAEGGYIYVYLIYGIYWLLNVVTGNKEHPEAVLIRG
                     INNISGSGRVGKELKLDKSFYGENLISSDRIWIEDAPEALYFFTATRIGIDYADEEWK
                     SKPWRFVMTSSEK"
     gene            complement(8970..9449)
                     /locus_tag="PLZ54_06195"
     CDS             complement(8970..9449)
                     /locus_tag="PLZ54_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOJ66552.1"
                     /translation="MKKTYTTDELFLKAASYCSISEHCVSEVEEKLSHWNVSENEKRK
                     IIDRLIKENFINEKRYAQSFVRDKFRLNKWGKIKIELALKSKGISNDEIKSAISTIDD
                     GEYQEMLASALKSKLSMLKYDYEYEKQGKLFRFAQSRGFETSIIDRVLRSLNQSAKE"
     gene            9618..10982
                     /locus_tag="PLZ54_06200"
     CDS             9618..10982
                     /locus_tag="PLZ54_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOJ66553.1"
                     /translation="MDTKGAPITLGTEKIGKLLIQYSLPAIIAMTASSLYNITDSIFI
                     GHGVGAMAISGLAVTFPFMNLSAAFGSLVGVGAATLMSVRLGQKEYGTANRILGNVFV
                     LNVIFGVAFMTVSLIFLDPILKFFGASDTTLPYAHDFMVVILIGNVFTHIYLGLNGIL
                     RSIGKPGKAMYATLLTVLINLILNPLFIFVLKWGIKGSALATVISQIVMLAWQIRHFS
                     NKNNFVHLQKGIFRLDKKIVLDSLTIGLSPFIMNAVASVIVIVINQTLIRYGGDLAIG
                     AYGIINRVSFLFVMIVMGLNQGMQPIAGYNFGAKNFNRVNEVLKLSIFIAMGMMTLGF
                     IIVEFFPHAVASLFTSDKTLIQLSIEGLRIVFVLYPMVGFQMVITNFFQSIGMSGKAI
                     FLSLTRQLLFLLPFLLVLPTFYGLKGVWYSMPAADFLATIVAAYMLFVNFGSFIKNQN
                     RDHK"
BASE COUNT         3457 a         1898 c         2193 g         3610 t
ORIGIN      
        1 aacgcccgac ggtaacatta aagttttgta taagattcag attccattat atctcaaacc
       61 gggcagctat tacgaattta aggacaccat tcccgatgcc ggtgaagata ttaattcggc
      121 tttggaggat gtagatgtta atagtggatc gattttcctc accgttacta acggacttcc
      181 cgtaaaaggc aaattaacgt tggttttgaa ggattcattg ggaagagata tttctactac
      241 ctttaaaaaa gaatatgata ttgatgctcc gcaagtagat gctgaaggtt ttgtggtgga
      301 aagcagcatt acccctcaaa aaatagaaat cgccttaaac aacgatcaac tgaatgactt
      361 caaacgttca aaagatattg tttataaatt cagaatcgac agcaaagatg ccaattcctt
      421 tattcatttc agaaaatcgg ataccttcaa ggtaaaactt ggtttgtttg taaatggaaa
      481 agttagttat gattcaaata acaacgatta ataaaaccga ataaccatga aaaagattat
      541 tatttcaata ctgacaggaa tatgttgctt gacctgcatc ttttctacat cagctcagca
      601 ggtgaattcg ttatatttta tggaaaattc gcccatccgt tcttatttga atccggcttt
      661 tcaaccaacc ggtagttttt atttgggatt acccgtgttg ggttatactt atttgagttt
      721 gggaaacaat tcgttgacgc tgaaggattt ggtttacaat aaaaatggag agaccatctg
      781 gtttttaaat ccggaaggtg atgtcgataa attttataaa tctttgcgtt cctcgacgtt
      841 gctcaatacc aacgaacaac tgaatctttt aaactttggc tttcgtgccg gcagtgctta
      901 ttggacattc ggaattaccg agaaagtaga aggaaatttg gcattgccta aggatatgtt
      961 taaactattg ctttatggaa cacccgaaca agaaaacaat tatttcaatt tcaaggattt
     1021 gaatgccaat gttatggctt acaccgaatt tgcgctgggg tattccaaaa taattaatga
     1081 ccaatggaca gtgggcggaa aattgaaatt tttggccggg aatgccattg taacttcttc
     1141 caacgattat tttaatttga atgccggaat cgaacagtgg aatatgaaag gaacaggttc
     1201 tttgaatatt gctgttcccg gagaagtaac gattggcgat aaatttgaat ctatcgatta
     1261 tactgaaccg gaagatatgc agagttggct gaagccttcg ggtatgggtt tagggttcga
     1321 cttgggattt acatataaac ctttggaaaa tgtaactctt tccgcatcct taattgacat
     1381 aggaacgatt aagtggaaca aaaacatgaa aaaggtagcc tacaatatgg attatacctt
     1441 tgatggactt gcaaaaatag acaatttgga ttctgtagat atggatgcat ttttggatac
     1501 gttgtccaat gcctttaaaa attccttcac aaccgaagaa aaagcaacgg cattctctac
     1561 tgctacttct ccaaaattca atttaggtgc agaatatgct tttgcaaaca acaaactcag
     1621 tctgggttta ctttctacta ccaccaaagt gaaaagcaac atgtatcagg agctgacagc
     1681 tgcgttcaat atgcgtccaa gtaattggtt caacttatct cttgcctatt ctctgttgaa
     1741 tgggagaatg agcaatattg gagcggcttt gggaataaga accggatttt tctattggaa
     1801 tctttccagc gactatgttt cgttagataa tgcacctttg ttattgagcg atattgattc
     1861 ttccttgcca tcaaccaaaa ttccaattcc ttacaacagt aaagggctga atttggcatt
     1921 gacggttaat tttgtttttg gtaacaaagt cgataaagat catgatggag tggtggacaa
     1981 aaaggataaa tgtcccgaca cacccaaagc agcacgcaag aaagtggatg caaacggctg
     2041 tccgctggat acggatcagg atagtattcc cgattatctg gatcaatgtc ccgatacgcc
     2101 attggctgct cgtggttttg tggatagcaa gggttgcctt ttggataccg ataacgacag
     2161 cattcccgat tatctcgaca aatgtcccga cacaccattg ggagtagccg tagattcttt
     2221 gggttgtcca ttggatacgg acaaggatag tgttcccgat taccttgatc ggtgtcccga
     2281 tacaccactt gaagctcacg ggtttattga tgaaaatggt tgcttattgg atacggatca
     2341 ggatggagtt cccgattatc tggatttatg tccgaataca ccattggaag caataaattt
     2401 cattgataaa aatggctgtc caatggatag cgatagcgat ggcgttcccg attatttgga
     2461 ccagtcacct aatactcctc tagaagctcg aggattggta gatgaaaaag gtgctccccg
     2521 cgataccgat ggtgatggcg tttatgattt caaggatgat tgtccaaaaa tccccggcga
     2581 cagcaccaat catggttgtc ctgaagtgaa aaaagaggtg cgcacacttt tccaaagagc
     2641 attaaaaggc atccagtttg ataccggaaa agatattatc aaaccttctt cttatcccat
     2701 tttggaccaa atcgctcaag tattgaaaga aaatccgacc tatttggtag aaattcaggg
     2761 acataccgat aatgtaggta atcctcagaa aaatatggaa ctttcagaaa aacgggctat
     2821 agccgtgaaa aaatatcttg tttctaaagg tgtggaagaa aacaggataa cggcaaaagg
     2881 ctatggagac accatgcccg ttgcgtccaa caaaactgct gcaggcagag cattaaacag
     2941 aagagtagag tttattgtaa cttttgagga agtgaaattc gaataaggaa tatttttctt
     3001 ttatagttgt tcgattattt ttaaggcatt tcaatcgtaa agtttgaagt gccttaaaat
     3061 ttttaggcaa ataagttttt aatatcctga cgccagtaca tcggccagat gaatgccaca
     3121 aatggataaa ttatgttttt tgcagtaagt attgatattc ataaggcatg aggcttccgt
     3181 gctcacaata aattcggctt ctgttctaag actgttggca acttttcgtg ctgtcatgga
     3241 agtggaaatg gcatgatttt ttacggcaaa tgtgcctcca aaaccacaac acgtatcatt
     3301 ttcgtccatt tcaaccaatt gcagtccttc tacctgtttg agcagttttc ggggctcatc
     3361 cttcaatcca tattcacgta aggccgagca agcatcatga aaagttattt tgtgagggaa
     3421 aacagcgcct aaatcttctg tttttaaaat atttaccaga aaatcggtta attcgtacaa
     3481 ctgacttttg atttctttaa atttttgcag tagttcgggg ttattcgcaa gtaatttgtt
     3541 ataatgatgc aaaataaatc cggtgcagga gccggaagga gaaactatag gcatatccga
     3601 attaaaatcg caaagaaatt tttctgccaa ataagctgtt tctttccaat aaccactgtt
     3661 gaatgcaggt tgtccacaac aggtttggtt ggaattgtag tgaactcgac aaccgacttt
     3721 ctcaagaatt tttataacgt taaaacctgt ttgcggataa agttgatcaa tataacaagg
     3781 aatgaataat tgaacatcca tgtatgtaaa aatttagaaa aaattttttg aatcagtacc
     3841 ataaatcgac ataaacagga agatggtcgg aaaaaccacc ctgatacttc atgcctacgt
     3901 aagttcggaa aggctgtttt cctaaaaatg tccggtcatc ttccagtaaa aaatcggctt
     3961 caaaaatgtg cgcatcactt ggaagagtat aaaccgaatt ggttggggtc aacaaatttc
     4021 ctgaaacaat gatttggtcg agtgtccccc attctccatt gtgtttgtgc gagccttttc
     4081 ctttcaaatg catcgggaac attaaattgt acaaattttt ttgtaaaaac ggctccgttg
     4141 gaggcagagc tttcagcgtt tccgacagac ttcgatttgg cgggtaatcg ttgaaatctc
     4201 ccatgataac aatatttgct ttattgttgg acgaaaacaa actgtctact tttgaacgca
     4261 atacggaagc tacaaatatt cttctttctt ccgattccaa ttcgcctccc agcctcgaag
     4321 gaaaatggca cacaaagaca tgtagtgtat ctcccgaagg aattattcct gaagcaaaca
     4381 aaatatcgcg tgttttaatt tcgggaaatt gcggaaagtt tatgcgtatg gcttcatcgt
     4441 gaaaaggttt aaatacgtaa ggttggtaaa gaagtgcaac atccacgcct ctggcatcag
     4501 gcgactcgtg atggaggaat ttgtatttta aatttttcag gccggcataa cgggtcaagt
     4561 ctaccagaca tttttcgctt tccacttcgc atagtccaac taatgccgga ctttcccagc
     4621 caccaatggc gcagattact ttaccgatgt tggcttgttt ctttttatat tttgttgtat
     4681 tccatgctct tattccattt ggcaggtatt cttggtcgtc agtaagcgaa tcgtccacgc
     4741 agtcgaaata attttcaaca ttgtaagaaa caatcttaaa gtttcgtttc tcttgacaat
     4801 aaagtcctac agcaaaaaga aaacaaattg ttgttgctaa aaaaactctt tttttaccgg
     4861 aggtaatcga ggacttcatg gtgaagatgt ttctttgaaa attttttaac cttgaataat
     4921 ttattcatcc tttttcgccg gcatccattc gtaagcgcct gcatcgggtg ctccatcctg
     4981 caaacggtta attccattta aatcgacagg acagttttcg gcagcaatat tgtaatcggc
     5041 caagcctctt aaaggagaaa cagaatccgg ttcgaaattg aaataagttc cttttttgta
     5101 atcaaaataa atatttttaa aaacggtatc gtttttctct gaccagcgga tattggaaaa
     5161 ttggggcaag tctaaggcat tgggcctacg aatgtaacaa tgggaaaaag ttcctttgta
     5221 caatttaggg aaacgggtgg caagagtaaa ttcgttttcc atactgccgg ctattgcaca
     5281 atttaaaaaa gtagtccgca tgggtgcaat gcggtttaaa tccataatca taacggccgg
     5341 ttctttgtcg cgcagaattg gctgaactga actgttgtta aaataatttg ctatcgtgca
     5401 atgaatgaaa gtatgctgtc caccgttgag ataaacgcaa aaactgctgc tgttggaaat
     5461 ttctgagttc aaaaccttca cattgccgtt ttgaaccacc agtccgtaaa gcaaaaagtt
     5521 gtgtatccga cagtttgaaa tctcaagttc cggtaattga tttttatcct gattggaaaa
     5581 ataaattcca acgtagccac tatttaaatt tacgttgtgc agaacatgtt ttcctttatt
     5641 ccaaagcaaa taaattcctc cccattgccc ggcaacgaca ttgtacaata cgggcgtttc
     5701 aaattttatt ttgtcaaacc tatctccacg catggtgatt ggcttttcaa aagttccgtc
     5761 agccattaaa tttccataaa caataagatt ggcattgttg tgaaaatata tttttagtcc
     5821 cggatccagc ctcagtgttt ttgcactgtc gacggcgagg tatccgaaaa cgagataagg
     5881 cttttcggct gtcagtgtcg tgtcgtttgc aatgagttta tttctgaaaa tgattgcatc
     5941 ttgtccaatg gcctgcaagg taattgtttt tatattgcca tttaccagaa aaattaatgc
     6001 atcttctatc agtaagggca aattgttgtc aagtggatcg acgttgactt gaacaaaaat
     6061 atacaggctg tcttttgcgc caatttctat atcggcaaac tgattgtttt cattgttttt
     6121 accatctaca ttgattttaa aggccgatga tttaccgctt gccaaaaaaa tgctcgaaat
     6181 ccgtatggct ttgttattgg gattgtaaac cataattctg gaagttgcac taccaatgga
     6241 tgtaaaggtg gtgtcgaatg tcaatgtgtc cgtcgaaaat agaacagtat ctgcgggatt
     6301 ggtagtaaaa tcttcctggc atgaagagaa aaaattaatt attgcccaaa gaataatcag
     6361 ttttgtgaag aagaaaactg tttttttaaa tttgaacatt ctatcgttgg tttttgctgt
     6421 tttaaatgga tgttaagctg gatttgtttt tgcaaattta atgtaaaaga tgactttatc
     6481 ataatattgg tttatatttg tgcaacaaaa aacttaaatc attctatcat ggcaagcaga
     6541 aaagaattga aaaaacaaat aaatgatctt acgggtcagt tggcattgga aattttattt
     6601 cgtggcactg ttatgcaggt tttggaacct gaaaaaatgt atgctttgct atcaaaagtt
     6661 atgatcatga acaggcagtt tatttcaagg gtaaatgcat caaaaaggat aaaaagttca
     6721 aaagaagtga aagatttcta tcaacaattt tttaccgatt gggaaaagaa tgtgggagaa
     6781 attattcaag agttctaatt tttctttcag cggttatgca ctctgacatt ttgaaactta
     6841 ctcgaaatta aaagaaaggg ttatcatttt attggtaagt ttggaaatgg cagtggaata
     6901 aattttatct tcttcggtta atcctcctgc atttctttgt tcgagaggcg taagcatgtt
     6961 gttgaatcca atggcaaatt ttaattcggg tgccaatttg aaaaaactga aatataaatc
     7021 acatcctacg ccaaattcaa caaaataatc catcggtttt agtaagatgg gtttttcctt
     7081 gtttcttcca aaatcaaaat acactcctcc gcctcccaac agatagggac gataattgtc
     7141 tttcctttca gcactgtatt ttaaatatac ggggaagctc agtggaatag agttgacagt
     7201 tgtggagcca gttgtacttg tggtatcttt ccgataagtg atgtctcttt cggaaaaatg
     7261 taaagttgga ttgaagcgca gattccaata tcgtgccaat cgaagattgg ttatcacacc
     7321 aaccgaaaat ccgggttgca aggcagatac atctgtgtaa tacctgctcc cattaataag
     7381 tgtatctctt ggcgttattc caaaatcagc cgtattcaat cctaaagaaa acccgaaatg
     7441 aatgagtttg tcatctacgt aaggtaaatt tccttgagct ttaactttag gaaaaaacac
     7501 cgaacaaaaa gttaagataa aaataaaagc aaaaatattg ttttttaaca tactgatttc
     7561 ctaagaattt tcacgttggg ctttgctata aaaaacgctt aaaagttgat attattgccg
     7621 gtttaacgag cgaggattga gaacaaataa taaagaagaa aaggcaagaa attttacaaa
     7681 ttggaaaaat agtaataata gacaatgatt tgactgagtg tcaaaccatc aaatttccga
     7741 attcttcgtc catagctgtc atataaagca tttccggaaa ctgtagccaa ctttgttccg
     7801 agtgcatcat aaaaatcgtt attttcgact ttcccaattt gtttcccgtt gccatcgtaa
     7861 agataacttc cgtcgatttt ccctatcagg ttttctgctg cgtcataaac aaaaacgctt
     7921 tcaacttttc ctttcaattt tgaattattg tcgtaataat tttgtccaat aatttttcca
     7981 ataggcttcc ctgcagcatt gtagatttgt tgtcctatta atgcagaatt tgaaaaaagg
     8041 aacagaagaa atagtatctt tttcatctca aaagactttt agtgaataca aattaattga
     8101 aaataaattg aaaaaacaaa taatgagtca ttttttattc tgcttcagaa aaaatttgaa
     8161 tttaggtgtt gttttattca aagttgtttt ttcaatggga gttagatact acactgaaat
     8221 tttagttgtt aattgagata tttgatgtaa atttgcatta aatgcagtgt aaaccgacaa
     8281 caaattaatt tttttgagtt tataattttt tatgaatcga ctggatgcgg ttttttttgc
     8341 ttccgatgcg attacggttg cccgtcaact attgggaaaa actttagtaa gaaagtggga
     8401 agatggtaca atttcccgat acaaaataac agaaacggaa gcttattttg gagaagaaga
     8461 taaggcttgt catgcctgca agggaaaaac tcagcgaaca cagattatgt atgccgaagg
     8521 aggttacatt tatgtttatc tgatttatgg gatttattgg ttgctgaatg tggttacagg
     8581 caacaaagaa catccggaag cagtactgat aaggggaatt aacaatattt cgggatcagg
     8641 aagagtggga aaagaattaa aacttgataa aagtttttat ggcgaaaatc taatctcttc
     8701 agatagaatc tggattgaag atgctcccga agcgttatat ttttttacgg ctacgcgcat
     8761 cgggatcgat tatgccgatg aagaatggaa atcaaagcca tggcggtttg taatgacatc
     8821 atccgaaaaa taaaatttct tttccccggt gatttgataa tggagatttg gaacaactca
     8881 attattgtaa agagatctgt tattataatt tttaagaagg tttttatttg atatgttttg
     8941 caacttaaaa ttttttttac cttttctgac tattctttgg cggattggtt cagtgaacgc
     9001 aaaacacgat cgataatgga tgtttcaaaa cctcgacttt gggcaaatcg aaaaagttta
     9061 ccttgttttt catattcgta atcgtatttg agcattgata gttttgactt aagtgctgaa
     9121 gcaagcattt cctgatattc tccatcatca atggtagaaa ttgcagattt tatttcatcg
     9181 ttagatattc cttttgattt caaagccaat tcaatcttga tttttcccca tttatttaat
     9241 cgaaatttat cccgaacaaa cgattgggca taacgttttt cattgataaa attttctttt
     9301 attaatctat caattatttt tcttttttca ttttctgaaa cgttccaatg gcttagtttt
     9361 tcttccactt cagaaacaca atgttcagaa attgaacaat aggatgctgc ttttaaaaaa
     9421 agctcatcgg tggtatacgt tttcttcatg ataaaaagat ttttttgtaa atgataaagg
     9481 aatacaaaaa taataatttt caaaaaaatc ggactgtttt catggttttt tctatatcag
     9541 acgaaagggt attttcaaaa ataaatgaag gaaaacaagc acataatttg taaatttgca
     9601 ttcctaaaaa tttaaaaatg gatacaaaag gagcacccat aacattagga acagaaaaaa
     9661 ttggaaaatt acttatccaa tattctttgc ctgccatcat tgcaatgact gcatcatcgc
     9721 tctacaacat aaccgacagt attttcattg gacatggtgt aggtgcaatg gctatatcgg
     9781 gattggcagt aactttcccg tttatgaatt tgtcggcggc tttcggatcg ctggtaggcg
     9841 tcggagctgc aacactcatg tcggtaaggt tgggacaaaa ggaatatggc acagcaaaca
     9901 gaattttggg aaatgtcttt gtgttaaatg ttatttttgg agtggcgttc atgacggttt
     9961 ctttaatttt tctcgatcct attctgaaat tttttggcgc tagcgataca actttgcctt
    10021 atgcacacga ttttatggta gtcatactta tagggaatgt atttacacat atttatcttg
    10081 ggttaaacgg tattttgcgc tcgataggca aaccgggaaa ggccatgtat gcgactttgc
    10141 tgacggtatt gataaatttg attttaaatc cgttatttat ctttgtttta aaatggggta
    10201 tcaaaggaag cgctctggcg acagttattt cgcaaatagt aatgcttgct tggcaaattc
    10261 gtcatttcag caacaagaat aattttgttc atttgcaaaa gggaattttc cggttggata
    10321 aaaaaattgt actcgattcg ttaaccattg gcttatctcc tttcataatg aatgctgttg
    10381 ccagcgtgat tgtaattgtt atcaatcaga ccttgatacg ctatggtggc gatttggcta
    10441 ttggggcgta tggaattatt aatcgggtca gttttctttt tgtcatgatt gtaatgggac
    10501 tgaatcaggg aatgcaaccg atagccggat ataattttgg agctaaaaat tttaatagag
    10561 taaatgaagt tttaaagctc agcattttca tagctatggg aatgatgact ctcggcttca
    10621 ttattgtaga gttttttcct catgctgtgg cttctctttt cacaagcgac aaaactctta
    10681 tccaattgtc gatagaagga ttgagaatcg tatttgtttt gtatccgatg gtaggttttc
    10741 aaatggttat caccaacttt ttccaaagta tcggaatgtc aggcaaagcc attttcctgt
    10801 cacttacccg acaattgttg ttcttgttgc cttttctgct cgtattacca actttttacg
    10861 gattaaaagg agtctggtat agtatgccgg ctgctgattt tctggctacc atcgttgcgg
    10921 cttatatgct ttttgtgaat ttcggaagct ttattaagaa tcaaaataga gatcacaagt
    10981 aaaaaataac tttgtgatta aactaatttc gaattatttg caacggatgt taagcataga
    11041 tttaatttac ttcgccattg attgagaaaa tctttgtttt tgcgtaacat tcgagtgttt
    11101 ggtctgataa aatgagattg tgccaaatgt ttaatgattt tcatcagaat ataattta
//
LOCUS       DAOSCH010000069        10960 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_3967_length_10960_cov_5.149931, whole genome shotgun sequence.
ACCESSION   DAOSCH010000069 DAOSCH010000000
VERSION     DAOSCH010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10960
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3967_length_10960_cov_5.149931"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            436..2829
                     /locus_tag="PLZ54_06205"
     CDS             436..2829
                     /locus_tag="PLZ54_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOJ66554.1"
                     /translation="MNKKSLSERDICTKFITPALEKAGWDTQLQILEEVSFTDGKIYV
                     RGKITARGTRKRADYILYYKPNIPIAIIEAKDNNQSVRAGIQQGLDYARILDIPCVFS
                     SNGDGFLFHDRTATDGNIEKEIGLDEFPSPEQLWEKYKKYKGIATPEAEKIASQDYYF
                     DGTNRKPRYYQQIAVNRTVEAIASGQNRILLVMATGTGKTYTAFQIIHRLWKSGAKKR
                     ILFLADRNALIDQTRRGDFKHFKDKMTVVKHRQIDKSFEIYLALYQGLSGTDESANVY
                     KQFSRDFFDLIVIDECHRGSAKDDSSWREILTYFQNATHIGLTATPKETNEASNTEYF
                     GDPVYTYSLRQGIDDGFLAPYRVLRIALNVDAEGWRPEQGKTDKDGNEIEDRIYNRRD
                     FDKNLVIEERTDLVAKKLTEFLKGYDRFAKTIVFCVDIDHAERMRTALARHNPDLVAE
                     NYKYVMQITGDNDEGKRELDNFINPEEKYPVIATTSELMTTGVDAQTCKVIVLDSNIN
                     SITKFKQIIGRGTRINEEYGKLYFTILDFRNATDLFADPAFDGDPIRIKPVTEETDLG
                     GIIDEEETQEEPIIDIETGEEIILEQPEIRYPEPIFPETEAREPRQKVYVNGVDVSVL
                     ISRELHFDQHGRPITTSLKDHTKEIIKEQFASLDDFLNKWNNSDRKEAIIAELIEQGV
                     MVEALYEAVDKQVDLFDLICHVAYDQPPLTRKERANNVKKRNYFTKYGDQARKVLEAL
                     LEKYADQGIENIENIQILTVPPINEFGSVTEIIKAFGSREEYEKAIRDLENELYKVA"
     gene            2872..4173
                     /locus_tag="PLZ54_06210"
     CDS             2872..4173
                     /locus_tag="PLZ54_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ66555.1"
                     /translation="MIKSVSIQNFRSIKDPITLDLSKSNRKKGDLSNYISISNTEIAT
                     TSVIYGANASGKSNLLRAFKALEYLVLNSAKFDPEDKIGPFEPFRLAKGFEKKMVNIE
                     LEFFIDQIRYIYSLAFGEKLIEQEKLLFFPMGKEATLFNRVKGREIQFGEYFKGEKKT
                     IEKLTLPNQLFLSKAAENNAESALPVFHFFKNRIKVYPFLNQYNESGLERFYAKRLAD
                     EPDSPFAKKLNALICALDTGINSISAKETDWNSVKFPDNMPDNIRKKIQEDFKYEIKT
                     VHELFDESNKSSGLIDFDIDEESTGTRRLLSIGGIIIDALEKGSVLVIDEFEKNLHPI
                     ITSYLIKLFNEKIFNPKNAQLIFATHDVTQLNEELFNRDQVWFTQKNEFGVTELIRCS
                     DIKGLRLNAPLDKWYVAGRLGGTAIINDTNFVMAMQEEIEE"
     gene            4170..4766
                     /locus_tag="PLZ54_06215"
     CDS             4170..4766
                     /locus_tag="PLZ54_06215"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HOJ66556.1"
                     /translation="MRRAGQHILIIPEGWCEYNYAQALKHSLPRDKQRSISVEMPKPN
                     NENSAFQLLYKAEIMIKKARRDKNPYDAVWIFFDNDNQPNLAGFFQKLSNTPVQIAYS
                     CMCIEHWFIIHFENNRQAYQNAHQALQRIETLWQNIFNQPYHKTKINHFEKLNENLAT
                     AMERANTITLQAEADETPVANRNPFFTIQNFIQFFQSL"
     gene            4763..6181
                     /locus_tag="PLZ54_06220"
     CDS             4763..6181
                     /locus_tag="PLZ54_06220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HOJ66557.1"
                     /translation="MSNIAGTIKSIRDIFRKDPGLSGDAQRIEQLGWMIFLKLFDDKD
                     KEKEILDPKYKSPIPTELQWRNWAENDEGITGDELIQFINNKLFPQLKDLKVTADNRL
                     GVIIRNIFDGTNNYMKSGTTFRQAINKLNEIDFNSSKEHHVFNVIYEEILQGLAAKKD
                     TGEFYTPRAVTQFIIDMVNPKLGEKITDPACGTGGFLVCAIEHLRKQVKNVADRKTLQ
                     ETITGTELKPLPFMLSVVNLITHDIEVPQILNTDALSRELTSIRQADRVDIIVANPPF
                     GGVVGDGMETNFPLNYRTKESADLFLILFIQLLKDGGRAGIVLPDGSLTGDGVKQRVR
                     QKLLEDCNVHTIVRLPQSVFAPYATVNTNLIFFEKGKPTKEIWYYEHTLPDGVKAYSK
                     TKPISIEEFDPIKKWWNKRKESEVAWKVPIQTIIDRNYDLDIKNPNKKVEEVVYDRKA
                     IIEKLEESFNNSLLLLNELKVK"
     gene            6183..7496
                     /locus_tag="PLZ54_06225"
     CDS             6183..7496
                     /locus_tag="PLZ54_06225"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOJ66558.1"
                     /translation="MGWVKIKLSDVIKNREGRYKPNDPAIKGLKRIDKINFLGQIFLS
                     DKNSNTDMILIKKGDLVISGINVEKGALAIYEGEENILATIHYSSYIFDKTKVDIDFL
                     KIFLRSPDFIQLLKEQVPGGIKTEIKPKHILSLEVYFPETTREQKAIAQLFFEREAKL
                     ILLNKDLSHQLTLLENLNQAILQEAVQGKLVQQDPKDEPATELLKRIKAEKAKSGKNE
                     KPLPPIKPEEIPFEIPSNWVWCRLGEIIEYTDNLDIQKHLSPDTIINYVDIDAIDNHN
                     FVIREAKQKTVRELSTRARRVLKPNFIAYSTVRPYLKNIAVIEDDLENYIGSTGFNVF
                     KTLNVELKYAFYYLLTPYVNNSFKDLMVGFNSPSITNDQFEKSLFPLPPLSEQKRIVA
                     EIEKQLAKTKQLKEHIIANQQATEQLLKALLHQAFEVEEMEEVNS"
     gene            7498..10614
                     /locus_tag="PLZ54_06230"
     CDS             7498..10614
                     /locus_tag="PLZ54_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOJ66559.1"
                     /translation="MQTIDKDTFLRGFKVLSNQSFDLFLGAGASISSGIHSGSDLVWH
                     FKRELLSVSGKINGKKFQDLKVEANKKVIQSYFAEEDAKVTNHYSYYFEKCYPDPLVR
                     QEFLTNLIKDKKPSIGFMCLSALVSKQKINTVWTTNFDDLIEKAISKLDISCQVVSPE
                     NAKSVQNYRNDIPTVVKLHGDFRYDALQNTDAELQHLEENLHNYFLQAATQRGLLVVG
                     YSGGDESVLQTLEKALEKPNAFPKGLIWCIPKDIMPSERLSKLIDKAYAQNQRSGFMV
                     IDSFDYFLHELYTVCELKNEQIDSIANERFEQRRFFRLTQNQANTTPVLLNAIKATHF
                     PKTIFSTKTKINGEGKWKKLREVLKDSNIVAAFSKGETLSLFGNENEIKDVFKDYLTD
                     DLKPIDIQEHLFHQPDSFYIGMLYELIEKSLVNDFGLALFSRGRNIRKVFSVNHPLNQ
                     EEIKSIPSWLNLNIPKGYQVFEAFEFKVEFVNKDLFLLICPTIHIQTTTGEEPDRLTV
                     QNITNIITSNRWNKKYGEKVNFWFKELKRRKPDLTFRLGDFEIKLSDYYATAAQKMGN
                     FFCFDSYAKIKEPLIYFHHQDESKQSIHPINGLKILGPLEESFGTNGIASKIQLAIIA
                     PDTGFEKVKTHLESLLNPVSPTTEKEYLKDYPGFDAVFKKHLVIPNSIQNEFVVAINS
                     VEIRNYSAIQFYELIKSKIDKLALKNSEIDCVVIYIPNAWKNFRELKNEQTYFDLHDS
                     LKLYAVKKGLRLQFIEDKSIGYGDQAKVKWWLSLGIYVKSNGTPWKVKTDNTETAFVG
                     LGYAIRQNARNKVVLGSSQIFDGSGNGLRFLLQPIEKPVFYNKNPFMSKDDAFRLVTN
                     IRNTYHKIDPVIGLKKLVLHKTTHFTRDEMDGICNALEGIDNIELLQIQQFSNWRAIK
                     LRKKKDGSGHEFDGFPIDRGTIIQLDEFSFLLWTHGLVQSNELAKPYYQGKRGIPTPL
                     LIKRFRGTDPIETVANDILKLTKMNWNGAELYKTMPVTIDFSKRLSVMGKQLEELGNK
                     AYDFRYFI"
     gene            complement(10716..>10960)
                     /locus_tag="PLZ54_06235"
     CDS             complement(10716..>10960)
                     /locus_tag="PLZ54_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66560.1"
                     /translation="RFAAWRSGGILAQKFNRRTALEPTTKLSTEALNPPLRQTAVGSW
                     CSVVRDCIPLSLLSLCIIFCRVVRCVFYFFEALEIF"
BASE COUNT         3995 a         1972 c         2060 g         2933 t
ORIGIN      
        1 aacttgatag ggcagtgctt cgaaatgcca aacgtttcat atacgcaacc gttggcagca
       61 agtgtaaagg acgacccgac aacaacgaac agacgactaa aaacgataaa aaagtaaacc
      121 attttgacag gtaaacacag acatagaaac aatacaacaa acggacaacg agtaagccaa
      181 tactcattgc cgaccctatg ttttttattt ttttcccccc cgcatttttt aaaaacaaat
      241 aagcgaagcg attcacgagc accgctaaaa agcaatttaa acaagcgagt aattttacgc
      301 cagcccgcaa gtggcacatt tggttttgcc ccgacacaca aagccgaccc ttcagcaaaa
      361 ccaaaagagc cactttttag cccacgcacc gacaataatt tgtattttgc taatctatat
      421 tttgacaagt aagtaatgaa taaaaagagt ttatcagaaa gagatatttg caccaagttt
      481 atcactcctg ctttggaaaa ggcaggttgg gacacgcaat tgcagatatt agaagaagta
      541 tcatttacgg atggcaaaat atacgttaga ggaaaaatca cagcaagagg aacaagaaaa
      601 cgagccgact atattctgta ttacaaacca aacattccta ttgctattat tgaagccaaa
      661 gacaacaatc aatcagtaag agcaggtatt cagcaaggac ttgactatgc aagaatctta
      721 gatattcctt gtgttttcag cagcaacggt gacggctttt tatttcacga ccgcacagcg
      781 acagatggaa acattgagaa agaaattggt ttagacgagt ttccaagtcc tgaacaactt
      841 tgggagaaat acaaaaagta taaaggaatt gcgacacccg aagcagaaaa aattgcttcg
      901 caagattatt actttgacgg aaccaaccgc aaaccaagat actaccaaca aattgccgta
      961 aacagaaccg ttgaagctat tgcaagcgga caaaacagaa ttctgttagt aatggcaacg
     1021 ggaactggaa aaacttatac ggcttttcaa atcattcacc gactttggaa aagcggagca
     1081 aaaaaacgca ttctgttttt agcagaccga aacgctttga ttgaccaaac tcgcagaggt
     1141 gacttcaaac atttcaaaga caaaatgact gtggtaaaac ataggcaaat tgacaagagc
     1201 tttgaaattt acttggcgtt gtatcaagga ctttcaggaa cggacgaatc agccaatgtt
     1261 tacaaacagt tttcaagaga ctttttcgac cttattgtaa ttgacgagtg ccacagggga
     1321 agtgcaaaag atgacagcag ttggagagaa atattaacct atttccaaaa tgctacgcat
     1381 atcggtttga cagcaacacc aaaggaaacc aacgaagcaa gcaacacaga atattttggc
     1441 gaccctgttt atacctattc tcttagacaa ggaattgacg atggtttctt agctccttat
     1501 cgtgtgcttc gcattgcttt aaatgtagat gccgaaggtt ggcgacccga acaaggcaaa
     1561 actgacaaag acggaaacga aatagaagac cgcatttaca atcgaagaga ttttgacaaa
     1621 aaccttgtaa ttgaggaaag aacagattta gttgccaaaa aactaaccga gtttttaaaa
     1681 ggttatgacc gctttgcaaa aaccattgtg ttttgtgtgg acatagacca cgctgaacgt
     1741 atgcgaacag ctttggcaag acacaatcct gatttggttg ccgagaatta caaatatgta
     1801 atgcagatta ctggcgacaa tgacgaaggc aaacgagagt tagacaactt cattaatccc
     1861 gaggaaaaat atccagtaat tgccactact tccgaattaa tgacaactgg agttgatgca
     1921 caaacttgta aggtaatcgt gttggattca aatattaatt caatcaccaa gttcaaacaa
     1981 attattggac gaggaacacg aataaatgaa gaatatggca aactgtattt caccattttg
     2041 gatttcagaa atgcaacaga cctttttgcc gaccctgcat ttgatggaga cccaattaga
     2101 ataaaaccag ttacagagga aaccgattta ggcggaatta ttgacgaaga agaaacacaa
     2161 gaagaaccaa taattgatat tgaaactggt gaggaaatta ttttagaaca acctgaaatt
     2221 cgttatcctg agccaatttt tcctgaaaca gaagcaagag aaccaaggca aaaagtgtat
     2281 gtaaacggtg tggacgtttc ggttttaata agccgtgaat tgcattttga ccaacacggc
     2341 agaccgataa caacaagttt aaaagaccac accaaagaaa ttataaaaga acagtttgct
     2401 tccttagatg acttcttgaa taagtggaat aattcagacc gcaaagaagc catcattgcc
     2461 gaattgattg agcaaggtgt aatggtagaa gcattatatg aagcagttga caagcaagtg
     2521 gatttgtttg atttaatctg ccacgttgct tacgaccaac caccattgac aagaaaggaa
     2581 cgagccaaca atgtgaaaaa acgcaactac tttaccaagt atggcgacca agcccgaaaa
     2641 gtattggaag cattgttaga gaaatacgct gaccaaggca ttgaaaacat tgagaacatt
     2701 caaatcctta ctgttccgcc aatcaatgaa tttggctctg taactgaaat tattaaagcg
     2761 tttggcagtc gtgaggaata tgagaaagct attcgtgatt tagaaaacga gctatataaa
     2821 gttgcttaat tcggttttgt tttgtaattt tgcaccaaat aagcaaataa gatgattaaa
     2881 tcggtatcaa tacagaattt caggtcaata aaagacccaa ttacgcttga cctttcaaaa
     2941 tcaaaccgaa agaaagggga tttatcaaat tacatttcaa tatcaaatac tgaaatagca
     3001 actacaagtg tgatttatgg ggcaaatgca tcaggtaaga gcaatttact tcgtgcattc
     3061 aaagctttgg aatatttggt tcttaattct gcaaaatttg accctgaaga caaaataggc
     3121 ccttttgaac ctttcagatt agccaaagga ttcgaaaaaa aaatggtcaa tattgaatta
     3181 gagttcttca ttgaccaaat ccgatatatc tattcacttg cttttggaga aaaattaatt
     3241 gaacaagaaa agttgctttt ttttccaatg ggaaaggagg ctactctttt caaccgtgtt
     3301 aaaggaaggg aaatccaatt cggtgaatat ttcaaaggtg aaaaaaagac tatagaaaaa
     3361 cttactcttc ccaatcaatt attcctttcc aaggcggcag agaacaatgc tgagagtgca
     3421 cttccagtat ttcatttttt taaaaatagg attaaagttt atcccttttt aaatcaatat
     3481 aatgaatctg gtttagagcg attttatgca aagagattag cagatgaacc agattccccg
     3541 tttgctaaaa aattaaatgc tcttatttgt gcattagata caggtattaa ttccatcagt
     3601 gcaaaagaaa ccgattggaa ctcggttaag tttcctgata atatgccaga taatatccgt
     3661 aaaaaaatac aggaagactt taaatatgag attaaaactg ttcacgaatt gtttgacgaa
     3721 agcaataaaa gctcaggttt aattgatttt gatattgacg aagaatctac aggaacaaga
     3781 agactacttt caataggtgg aattatcatt gacgctcttg aaaaaggttc agttttagtt
     3841 attgatgaat ttgaaaagaa ccttcatccc atcatcactt cctatcttat taagttgttc
     3901 aatgaaaaaa tattcaatcc taaaaatgcc caactcatat ttgccacaca cgatgtaact
     3961 caattaaacg aagaattatt taatcgcgac caagtttggt ttactcaaaa aaatgaattt
     4021 ggtgtaacgg aactaattcg ttgttctgac atcaaaggtt tgcgactaaa tgcaccgctc
     4081 gacaaatggt atgttgctgg aaggttggga ggcactgcca tcattaacga taccaatttt
     4141 gtaatggcaa tgcaagagga gattgaggaa tgagaagagc cggtcaacat atattaatta
     4201 ttcccgaagg ttggtgtgaa tacaattatg cacaagcctt gaaacattct ctgcctagag
     4261 ataaacaaag aagtatttct gtagaaatgc ctaaaccaaa taatgagaat agtgcatttc
     4321 agcttttgta taaggcagaa ataatgatta agaaagcaag gcgagataaa aacccctacg
     4381 atgctgtttg gatttttttt gataatgaca atcagccaaa tcttgcagga ttttttcaaa
     4441 aactctcgaa tacaccagtt caaattgctt atagctgtat gtgcatagaa cattggttta
     4501 tcattcattt tgagaataat cgtcaagcct atcaaaacgc tcatcaagca ttacagcgta
     4561 ttgaaacatt gtggcagaat atttttaatc agccctatca caaaacaaaa attaatcatt
     4621 ttgaaaagtt gaatgagaat ttagcgactg caatggaaag agcaaatact attacgctac
     4681 aagccgaagc agacgaaaca ccagtagcta atagaaatcc attctttaca attcaaaatt
     4741 ttattcagtt ttttcaatca ttatgagcaa catagcaggc accataaaat caatcagaga
     4801 cattttccga aaagacccgg gcttaagtgg cgatgcacaa cgcattgaac aattgggctg
     4861 gatgattttc ttaaagctct ttgacgataa agacaaggaa aaagaaattc ttgaccctaa
     4921 atacaaatca cccattccaa cggagttgca gtggcgtaat tgggcagaaa atgacgaagg
     4981 aattacaggc gatgaactta tccagttcat caacaacaaa ctttttccgc aattaaaaga
     5041 cctgaaagtt acagccgata accgattggg tgttatcatc cgaaatattt ttgacggaac
     5101 aaataactat atgaaaagcg gaactacctt ccgccaagcc attaataaac tcaacgaaat
     5161 tgactttaac agcagtaaag agcatcacgt ttttaatgtg atttatgagg agattttgca
     5221 gggattggct gccaaaaaag atactggcga attttacacg ccaagagccg taacgcagtt
     5281 tatcattgat atggtaaacc ccaagttggg agagaaaatt accgaccccg cctgcggcac
     5341 cggtggcttt ttggtgtgtg ctattgaaca tttgcgaaag caggtaaaaa atgttgccga
     5401 ccgaaaaacc ttgcaggaaa ccattacagg cacagagcta aagcccttgc cttttatgct
     5461 gagtgtggta aacctgataa cccacgacat agaagtgccg caaattttaa ataccgatgc
     5521 actaagtcgt gaactcactt ctatacgcca agccgataga gtagatatta ttgtagccaa
     5581 tccacctttt ggtggtgtgg tgggtgatgg aatggaaacc aatttccctt tgaactaccg
     5641 caccaaggaa agtgccgacc tgtttttgat tttgtttata caattgttga aagatggcgg
     5701 acgggcagga attgtgttgc ccgatggcag tttaacagga gatggagtaa aacaacgagt
     5761 aaggcaaaaa ctgttggaag attgtaatgt gcataccatt gtgcgattgc cacaaagtgt
     5821 atttgctcct tatgccaccg taaacaccaa cctgattttc tttgaaaaag gaaaacccac
     5881 aaaagaaatt tggtattacg aacacacctt gcccgatggc gtaaaagcat acagcaaaac
     5941 caaacccatt agtattgaag aattcgaccc gattaaaaaa tggtggaaca aaagaaaaga
     6001 aagcgaagta gcttggaaag tgcctatcca aaccatcatt gaccgtaatt acgatttgga
     6061 tattaaaaac ccaaataaaa aagtggagga agtggtgtat gataggaaag caattattga
     6121 aaaattggaa gaatcattca ataattccct tttattgtta aacgaactta aagttaagta
     6181 aaatgggatg ggtaaaaata aaacttagtg atgttataaa gaatcgtgaa ggaagatata
     6241 aaccaaatga tccagcgatt aagggtttaa agaggattga taaaataaat tttttaggac
     6301 agatttttct ctctgataaa aattcaaaca ctgacatgat tctaatcaaa aaaggagatt
     6361 tggttatatc aggaataaat gttgagaaag gagctcttgc aatttacgaa ggagaagaaa
     6421 atattttagc aactattcat tattcatctt atatttttga taaaactaaa gtcgatatag
     6481 attttttaaa aatctttctc agaagccctg actttataca actcttaaaa gagcaagttc
     6541 caggtggaat aaaaaccgaa attaaaccaa aacatatttt atcgctagag gtttattttc
     6601 ctgaaacaac aagagagcaa aaagcaattg cacagctttt ttttgaaaga gaagcaaaac
     6661 ttattctatt aaacaaggat ctttcccacc aactcaccct cctcgaaaat ctaaaccaag
     6721 ccattttaca agaagcggtg cagggtaaat tggtacagca agacccaaaa gatgaacctg
     6781 ccaccgaact gctcaaacgt attaaagcag agaaagcaaa atcgggtaag aatgaaaaac
     6841 ccttaccacc catcaaaccc gaagaaatcc cgtttgaaat tccttcaaat tgggtgtggt
     6901 gtaggttggg agagattata gaatacacag ataatcttga tattcaaaaa catctttcac
     6961 ctgatacaat aataaactat gtagatattg atgccataga taaccataac tttgttatca
     7021 gagaagcaaa gcaaaaaacc gtaagggaac tttctacaag agcaagaaga gttttaaagc
     7081 ctaattttat tgcttattca acagttagac cttatctgaa aaacattgca gtaatagaag
     7141 atgatttaga aaattacatt gggtcgacag gatttaatgt ttttaaaact ttgaatgtag
     7201 aattgaaata tgctttctat tatttactca caccatatgt aaataattct ttcaaagatt
     7261 taatggtggg ttttaactcg ccaagcatta caaatgacca atttgaaaaa agtcttttcc
     7321 cccttccccc actttccgag caaaaacgca tcgtagcgga aatagaaaaa caattagcca
     7381 aaaccaaaca attaaaagaa cacatcatag ccaaccaaca agccaccgag caattgctaa
     7441 aagcattgct gcatcaggca tttgaggtgg aggaaatgga agaagtaaac agttagtatg
     7501 caaacaatag acaaagacac ctttttacga ggcttcaaag tgctttcaaa tcaatcgttt
     7561 gatttgttcc ttggagcagg tgcgtctatt tcttcgggca ttcattcggg aagtgatttg
     7621 gtttggcatt tcaaacgaga actactaagc gtaagcggga aaatcaacgg taaaaaattt
     7681 caagacttaa aagttgaagc caacaaaaaa gtaattcaaa gctattttgc cgaagaagat
     7741 gcaaaagtta caaatcatta ctcctactat tttgaaaaat gctatcccga cccattggtt
     7801 aggcaagagt ttttaacaaa tttgataaaa gacaaaaagc cgtccattgg ttttatgtgc
     7861 ctttctgcat tggtcagcaa acaaaaaatc aataccgttt ggacaaccaa ttttgacgac
     7921 ctgattgaaa aagccattag caaactcgac atcagttgtc aggtagtttc acctgaaaat
     7981 gccaagtcgg ttcagaatta caggaacgat attcccacag tcgtaaaact acacggagat
     8041 tttcgctatg atgctttgca aaatacagat gctgagttgc agcatttgga agaaaaccta
     8101 cacaactatt ttttacaagc agctacacaa agaggtttat tggtagttgg ttattcaggt
     8161 ggtgatgaat cggtattgca aacactcgaa aaagcattgg agaaaccaaa cgcatttcca
     8221 aaaggattga tttggtgtat tcctaaagac attatgccaa gcgaaagatt atcaaaactc
     8281 atagataaag cctatgcaca aaatcagcgt tcgggcttta tggtgattga tagcttcgac
     8341 tatttcctgc acgaactcta taccgtttgt gaattaaaaa acgagcaaat agattccatt
     8401 gccaacgaac ggtttgaaca aagacggttt ttcaggctta cacaaaatca agcgaatacc
     8461 actcctgttc tcctcaacgc tatcaaagca acgcattttc ctaaaacaat tttttctacc
     8521 aaaacaaaaa ttaacggaga aggcaaatgg aaaaagctaa gagaagtatt gaaggattca
     8581 aacattgtag ccgctttcag caaaggcgaa acactttcac tttttggaaa tgagaacgaa
     8641 ataaaggatg tattcaaaga ttatctaaca gacgatttaa aaccgattga catacaagag
     8701 catttgtttc atcagcccga ttctttttac attggtatgc tgtatgaatt gattgaaaaa
     8761 tcattggtga atgattttgg cttagcactt ttcagcagag gcagaaatat caggaaagtg
     8821 ttttctgtta atcacccttt aaatcaagag gaaattaaga gcatcccaag ttggttaaat
     8881 ctcaacattc caaaaggcta tcaggttttt gaagcatttg agtttaaggt agaatttgta
     8941 aacaaagatt tgttcctact catttgcccg accattcaca tacaaacaac cacaggcgaa
     9001 gaacctgaca gattaactgt tcaaaatata accaacataa ttacttcaaa ccgttggaat
     9061 aaaaagtatg gcgagaaagt caacttttgg ttcaaagaat taaaacgcag aaaacctgat
     9121 ttgacttttc gcttaggcga ctttgaaatt aaactttctg actattacgc aacagcagcc
     9181 caaaaaatgg gaaatttctt ttgctttgac agttatgcaa aaataaagga accgcttatt
     9241 tactttcatc atcaagacga atcgaagcaa tcaattcacc caataaatgg cttaaaaata
     9301 ttaggtcctt tggaagaaag ttttggaaca aatggcattg cttcaaaaat tcagttggca
     9361 attattgctc cagatacagg atttgaaaaa gttaaaacgc atttagaatc cttactaaac
     9421 cccgtaagtc caactactga aaaggaatac ttaaaagatt atccgggttt tgatgcggtg
     9481 tttaaaaagc atttagttat tccaaattca attcaaaatg aatttgttgt tgcaataaat
     9541 agtgtagaaa taaggaacta ttcagcaatt cagttttacg aattgataaa aagtaaaata
     9601 gataaacttg ctctaaagaa ttccgaaatt gattgtgtgg taatttacat tcccaatgct
     9661 tggaaaaatt tcagagaact gaaaaacgaa cagacttatt ttgaccttca cgattcattg
     9721 aaactatatg cagttaagaa gggtttacga cttcaattca tagaagataa atcaatcgga
     9781 tacggagacc aagccaaagt gaaatggtgg ctttcgttgg gtatttatgt aaaatcaaat
     9841 ggcacacctt ggaaagtaaa aactgataac actgaaacag cctttgttgg attgggttat
     9901 gcaataagac aaaacgcaag aaacaaagtt gttttaggta gcagtcaaat ttttgacggt
     9961 agtggaaatg gtttacgttt tttacttcaa cctatcgaga agccagtttt ctataacaaa
    10021 aatccattta tgagtaagga tgatgctttt cgtttggtaa caaatatccg aaacacatat
    10081 cacaaaatag acccagtaat cggccttaaa aagttggtgc tgcataagac aactcacttc
    10141 acccgtgacg aaatggacgg tatttgtaat gcacttgaag gcattgacaa cattgaactt
    10201 ttgcagattc aacagttcag caattggaga gccattaaac ttcgcaaaaa aaaagatggt
    10261 agcggacatg aatttgacgg gttccctatt gacagaggaa caatcattca attagacgaa
    10321 tttagtttct tgttgtggac acacggactg gttcaaagca acgaacttgc aaaaccatac
    10381 tatcaaggca aacgtggcat tccgacacct ttgttaataa aaagatttag aggaacagac
    10441 ccaattgaaa cagtagccaa cgatatttta aaactgacga agatgaattg gaacggtgca
    10501 gaattataca agacaatgcc tgtaacgatt gacttttcaa aaagactttc agtaatggga
    10561 aaacaattag aagaacttgg aaacaaagcc tatgatttta gatacttcat ttagccaacg
    10621 cattttgcaa gcacattgcc aaagccgcac aagcccaagc atagaccaaa gctttgtcaa
    10681 agagcttgca aagccaacgc aagacaaaat tgtttttaaa aaatttccaa cgcttcaaaa
    10741 aaataaaaaa cgcaacgcac aacccggcaa aaaatgatac ataaactcaa caacgacaat
    10801 ggtatacagt cacggacgac agaacaccag ctgccaacag cagtttggcg taatggcggg
    10861 ttcagtgctt cggttgacaa ttttgtggta ggttcaagtg cagttcttcg attgaacttt
    10921 tgtgctaaaa ttccgccact acgccaagcc gcaaaacgtt
//
LOCUS       DAOSCH010000070        10715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4069_length_10715_cov_6.240244, whole genome shotgun sequence.
ACCESSION   DAOSCH010000070 DAOSCH010000000
VERSION     DAOSCH010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10715
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4069_length_10715_cov_6.240244"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLZ54_06240"
     CDS             <1..346
                     /locus_tag="PLZ54_06240"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOJ66561.1"
                     /translation="DTMRNVPKVPYALWGSFSDLEKVTSEMRLPISGKAGSFVNVLPD
                     EKNNALEISKKTVYRQLMPDLKGMGAKDAVFLAEKLGLKVMIIGRGKLVSQSISPGTK
                     ITKGQILSLHFQ"
     gene            364..1809
                     /locus_tag="PLZ54_06245"
     CDS             364..1809
                     /locus_tag="PLZ54_06245"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOJ66562.1"
                     /translation="MILKVALEKIFPTEIIGNTEMEISGVQSDSRKVEKGNIFVATRG
                     TNVNGHNYISPAIEKGASAIVCETFPETIQQGIVYIRVENSLEALGLLACAWYGNPSE
                     KLTLVGVTGTNGKTTIATLLYQMFRQLGYKAGLLSTVRNYVVDEPVEATHTTPDPLEL
                     NHLLAKMVEAGCQYAFMEVSSHAIGQRRIAGLLFKGGIFTNITRDHLDYHLTFENYLK
                     AKKKFFDDLPENAFALTNLDDKNGLVMLQNTKAKKYTYSLRSMADFKTRILEQDFEGM
                     LLEMNGKQVHVSFVGKFNASNLTAVFGAAVLMGEEETEVLRILSLLHPVSGRLETLHA
                     PSGYTVIVDYAHTPDALNNVISTIHQVLQNKGRLITVVGCGGNRDKGKRPLMAKEAVT
                     GSWKTILTSDNPRFEDPMDILNDMIAGLTNEERKTSLVIPDRRQAIHTACSLAQPGDV
                     VLIAGKGHEDYQIIKGEKHHFDDREVVKECF"
     gene            1904..3166
                     /gene="mraY"
                     /locus_tag="PLZ54_06250"
     CDS             1904..3166
                     /gene="mraY"
                     /locus_tag="PLZ54_06250"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOJ66563.1"
                     /translation="MFYHLFRYLDKFYDIPGAGMFNYISFKTGLSFIFALLLSTLFGR
                     RIIDFLQKKQIGETIRDLGLEGQLSKKGTPTMGGIIIINAILIPTLLFADLTNIYIIL
                     MLITTVWMGAIGFVDDYIKVFLKNKEGLSGKAKIVGQVGLGLIVGLVMYFSPDVVMRE
                     NTEIRGELRVEDVIYSKKDIKATRTTIPFVKNNDLDYADAFKNAGKNAQKYGWILFVL
                     VAIFIVTAVSNGANLTDGLDGLSAGSSAIMGGALGVLAYLSGNINYSGYLNIMYIPGT
                     GELLVFAGAFLGATIGFLWYNSFPAQIFMGDTGSLTLGGIIAVFAIAIRKELLLPILC
                     GVFLAESLSVICQVSYFKYTKKRYGVGKRIFKMTPLHHHFQKPGNAGIEALIQRPLQA
                     VPEPKIVVRFWIVSMILAALTIITLKIR"
     gene            3206..4540
                     /gene="murD"
                     /locus_tag="PLZ54_06255"
     CDS             3206..4540
                     /gene="murD"
                     /locus_tag="PLZ54_06255"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOJ66564.1"
                     /translation="MKRIVVLGAGESGAGAAVLAKQQGFDVFVSDLSEIKENYKELLN
                     QYQIEWEEKQHTEALILNADEVIKSPGIPDKAPIIKNLKEKNIPIISEIEFAGRFTNA
                     KMICITGSNGKTTTTMLTYHILKNAGYNVGLAGNVGQSLAYQVATSSRDYYVVEISSF
                     QLDGMYDFKADVAILLNITPDHLDRYEYNFQNYVDSKFRIIRNQTSEDAFIFWDDDPV
                     IQSEIAKRSILAAKYPFSLKKKEHCKAYIENNNLIIETSNNPLIMPVKELSLKGIHNT
                     YNMMAAGLAASAVNVKKEFIRQSLQDFQGVEHRLEYVATVKNVTFINDSKATNVNSCW
                     YALESMKTPVILILGGTDKGNDYSEIESLVKEKVKALVFLGVDNTPLHRFFDGKIDKI
                     VDVRSMEDAVRAAYAMAEEGDTVLLSPCCASFDLFQNYEDRGRQFKTCVRNL"
     gene            4579..5811
                     /locus_tag="PLZ54_06260"
     CDS             4579..5811
                     /locus_tag="PLZ54_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOJ66565.1"
                     /translation="MENFLKKYLKGNATIWIVFILLCLVSILEMYSASSTLAFKSSNT
                     AMPLLRHAGFLLFGVILAFLVHLIPYKYIRLLAYLGLFFSFILLILVYYKGQSANDAT
                     RWFEIGGLQFQPSELAKLSVIIVVADFMSRIKNEAADEKKYFRYILIVLAAICPLILF
                     ENFSTAMLLFGVVFLMMFIGGISRKKLGLLLLSITLFVLGGYAMIKTVPEDSMPKIFD
                     RAYTWSARIDRYTEEKKQEENKYVITDENRQVQHGRIAIARGGMLGVMPGNSVERDYL
                     PQAYSDFIYAIIVEEMGLVGGVIIILFYLVLLYRAGRIAVKSETVFPALLVIGLSLMI
                     VLQAFVSMAVATSLGPVTGQPLPLISRGGTSIIITCIYFGIILGVTRQLKGEQHVEEE
                     IPEEQERNFPVVDLNELS"
     gene            5894..7000
                     /gene="murG"
                     /locus_tag="PLZ54_06265"
     CDS             5894..7000
                     /gene="murG"
                     /locus_tag="PLZ54_06265"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOJ66566.1"
                     /translation="MPYRFIISGGGTGGHIFPAISIANALKKQLPNAEILFVGALGRM
                     EMERVPAAGYPIKGLPVSGFDRKNPFRNVKVLLNLRRSLQMARQIIRDFKPDAVIGVG
                     GYASAPTLKAASDLGIPTLIQEQNSYAGITNKLLAKKAARICVAYEGMERFFPKEKLV
                     LTGNPVRQELLSIPEKSKEAFEFFGFRPEKPTLLVVGGSLGARTINRSIISHLQELSQ
                     ADIQIIWQTGKFYIEEARKAAEPFSSPQLIVTDFVSRMDLAYSVADLVISRAGAGSIS
                     ELCLLGKPAILVPSPNVAEDHQTKNALALVQKEAAVMIKDEEAEKMLIPKALELIKDA
                     DSLNKLGQNIRKLAQKDSAERIAAEVLQLISKKN"
     gene            7047..8429
                     /gene="murC"
                     /locus_tag="PLZ54_06270"
     CDS             7047..8429
                     /gene="murC"
                     /locus_tag="PLZ54_06270"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOJ66567.1"
                     /translation="MDFTKYYFLGIGGMGMSALARYFNSKGFQTAGYDRVQSKVTADL
                     QTEGIQINFDPNVDQIPISFRYPENTLVVVTPAIPDDLPQLVYFRENGFTIKKRAEVL
                     GDITRKASTICIAGTHGKTTTSTLTAHLLYQSHISCNAFLGGIANNYNSNLLLSKDSN
                     LFVIEADEYDRSFHQLSPYMAVITSADPDHLDIYQTPEAYRESFEHFTSLISPGGVLL
                     MKKGVNIQPRLQKGVKQYTYSMNDGGDFYAENIHLVNHEFHFDFVTPSERISDIRLKI
                     PIRINIENSVAAIALAWLNGVTKEEIRTAIASYSGIYRRFNLVFRSEQTIFMDDYAHH
                     PKELKASILSIKELFPEKKITGIFQPHLYSRTRDFASDFAEALSLLDELILLDIYPAR
                     EEPIEGISSEMIFRNVKLDNKTLCSRENLLSLLKDKHPEVLVTFGAGDIDKLVPLIKK
                     QLMEQTNGNA"
     gene            8552..9301
                     /locus_tag="PLZ54_06275"
     CDS             8552..9301
                     /locus_tag="PLZ54_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HOJ66568.1"
                     /translation="MKPVFKYILITFGILLVTGYLAWALLTFPDPKANEVCNRFHIVF
                     LDQSGVKLISEKEVAEMIDIKGLNPMGKTLKHVRTEEIEELLTKHPFIKQAECFKTPS
                     GGVYLFLQQRHPKFRLAGNENFYVDDEKNIIPVSYNDAARLPVVSGLVTKSMAKGELF
                     DFVSYLEENPFWNAQIEQIYIRPDRKIELVPRVGTGIILLGNLKDYEKKLHKLKILYE
                     KGFNEFGWNYYKTIDLQYENQIVCTKTDNID"
     gene            9346..10608
                     /gene="ftsA"
                     /locus_tag="PLZ54_06280"
     CDS             9346..10608
                     /gene="ftsA"
                     /locus_tag="PLZ54_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOJ66569.1"
                     /translation="MNQNTYIALDLGSSRITAMAAEVLESGAVKVLATESKAADDVYH
                     GIIEHTSGAAFKISELLKLLQNSAKIRDIQQVCVALNAKSMKLSKSSTTRFIGQGKIV
                     TDALLDEMKEECERKNSDENNIVFDVIPVSFEIDGKITTDPVGQSGNQITGYYHVVKG
                     NARILQQLKSCFVRNQIQIEYAPLSVEALSTVLTEEEERQNGCALIDFGASTTTLAVY
                     KNDVLQDLLVVPLGSKNITYDIAELGIEESNAERLKCLTGVAMEKFVEKHVYVQVAPS
                     SGTDPVRISTLFLAQIINARLDEMTEPVFKVLEELSFPLESGIIITGGGAKLKFLKEF
                     VEEKSGLKVRWGNHSDWLEDQAPDYLADSSYSQLIGTILLANENRRLHPLKSENTTKK
                     GQKNKKKITERITDGFLRFFDDETTWNK"
BASE COUNT         3357 a         1999 c         2288 g         3071 t
ORIGIN      
        1 ggataccatg cgaaatgtac caaaagttcc ttatgctttg tggggtagtt tcagcgattt
       61 ggaaaaagtt acgtcagaaa tgcgactgcc tatttcgggc aaggcaggaa gttttgtcaa
      121 tgtgttgccc gatgaaaaaa ataatgcttt ggaaatttca aaaaaaactg tttacaggca
      181 attgatgccc gatttaaaag gaatgggagc gaaagatgct gtttttctgg ccgaaaaatt
      241 aggcttaaaa gtaatgataa tcggtcgcgg aaaattggtt tcgcaaagta tcagccctgg
      301 cacaaaaata acgaagggtc aaatcctttc tcttcatttt cagtaaatta aaaacaaaaa
      361 gttatgattt tgaaagtcgc tttagagaaa attttcccaa ctgaaattat tggaaataca
      421 gagatggaaa tttccggtgt tcagtccgat tccagaaaag tggaaaaagg gaatattttt
      481 gttgctactc gtggcaccaa tgtcaatgga cacaattaca tttctccagc cattgagaaa
      541 ggagcctctg ccatagtatg cgaaaccttt cctgaaacta tccagcaggg aatcgtctat
      601 attcgcgttg aaaacagttt ggaggcattg ggacttttgg cttgtgcatg gtatggcaat
      661 ccttcagaaa agctgacatt ggtcggcgtt accggcacca acggaaaaac caccattgct
      721 accttgcttt accaaatgtt ccgtcagctc ggatacaaag ccggactgct gtctactgtt
      781 cgaaattatg ttgtggatga acctgtagaa gcaacccata ccactcccga tccgttggag
      841 ttaaatcatc tgctcgcaaa aatggttgaa gcagggtgcc aatatgcttt tatggaagtt
      901 agttctcatg ccatcggtca gcgtagaatt gccggattgc tttttaaagg aggaattttc
      961 accaatatta cgcgtgatca tctggattac catttgactt ttgaaaatta cctgaaagca
     1021 aagaaaaaat tttttgacga tttgcccgaa aatgcttttg ctttaaccaa cttagatgat
     1081 aagaacgggc tggtcatgct gcaaaacaca aaagcaaaaa aatatactta ttctctcaga
     1141 tcgatggccg attttaaaac ccgtattctg gaacaggatt ttgaaggaat gcttttggaa
     1201 atgaatggca aacaagtaca cgtttcattt gtaggaaaat tcaatgccag caatctgacg
     1261 gccgtattcg gagcagcggt actgatggga gaagaagaaa cagaagtact gagaattctg
     1321 agtttgcttc atcccgtttc cgggcgattg gaaacgttgc atgctccgtc tggatatacc
     1381 gttattgttg attacgcaca tactccagat gctttgaata atgtgatcag caccatccat
     1441 caggttttgc aaaacaaagg ccgactaatt acggtagttg gctgtggagg aaatcgagac
     1501 aagggaaaac gtcccttgat ggcaaaagaa gccgtaaccg gcagttggaa aaccattctg
     1561 acttccgaca atccacgttt tgaagatccg atggacatac tgaacgatat gattgccgga
     1621 ttgaccaacg aagaaagaaa aacaagcctt gtgattcctg accgccgtca ggccattcat
     1681 accgcctgct cgctggctca acccggcgat gtggtgctga ttgcaggaaa aggacacgaa
     1741 gactaccaga ttattaaggg cgaaaaacat cattttgatg atcgagaagt ggtaaaagaa
     1801 tgtttttaaa agattttatt cggctataaa agaaaaaaaa ttcaaattac taaccaagac
     1861 tttcctgttt tcagaaaagg cgaagtcaaa aaaagaaacg gacatgttct atcatttatt
     1921 tcgctatttg gacaagtttt acgatattcc cggagcagga atgttcaatt acatttcgtt
     1981 caaaacgggt ttgtccttta tttttgcctt gttgctttcc acgctttttg gacggagaat
     2041 cattgatttt ttgcaaaaga agcaaattgg cgaaacaatc cgcgatttgg gattggaagg
     2101 gcagctttca aaaaaaggaa ctcccactat gggtggaatt attattatca atgctattct
     2161 tatcccgact ttactttttg ccgacctgac caatatttat atcattctga tgctcatcac
     2221 cactgtttgg atgggagcaa ttggatttgt tgacgattac ataaaagtat ttctgaaaaa
     2281 caaggaagga cttagcggca aagcgaagat agttggtcag gtaggattag gactgatagt
     2341 tggattggta atgtatttca gtcctgacgt ggttatgcgc gaaaacaccg aaatcagagg
     2401 agaattgcga gttgaagatg taatatattc aaaaaaagat atcaaagcta cccgaaccac
     2461 cattcccttt gtaaaaaaca atgacctcga ttatgcggat gcgtttaaga atgcggggaa
     2521 aaatgcacaa aagtatggat ggattctgtt tgtactggtt gctattttca ttgtaacggc
     2581 agtatcaaat ggtgcaaatt tgactgatgg attggatgga ctttcagccg gttcgtcggc
     2641 aattatggga ggtgcgcttg gcgtacttgc ctacttgtcg ggcaatatca actattccgg
     2701 ttacctgaat attatgtata ttcccggaac cggtgaactg ttggtctttg ccggtgcttt
     2761 tttgggcgcc accattggtt ttctatggta caattctttc cctgcacaaa tttttatggg
     2821 cgacacgggg agtttgacgt tgggaggtat cattgctgtt tttgccattg ccatcagaaa
     2881 agagttgttg ttacccattt tgtgcggtgt ttttttggcc gaaagcctgt cggtaatctg
     2941 ccaggtgagc tattttaaat acacaaaaaa aaggtacggt gttggcaaaa gaatttttaa
     3001 aatgacacct ttgcatcatc attttcaaaa acccggaaat gcgggaattg aagcgttaat
     3061 tcaacgaccc ttgcaagcag ttcccgaacc aaaaattgta gtcagattct ggattgtgag
     3121 catgattttg gctgcattaa ctattattac attaaaaatc agataatttc ttggaaaatt
     3181 tcataaaatt ttgttcaaaa aagaaatgaa acgaatcgtt gtacttggag cgggagaaag
     3241 tggagcaggc gctgccgtat tggcaaaaca gcaaggtttt gatgtgtttg tttccgactt
     3301 gtccgaaata aaggaaaact acaaagagtt gctcaaccag tatcaaatag aatgggaaga
     3361 gaaacaacat accgaagcgc tgattttgaa tgccgatgaa gtaataaaaa gtccaggaat
     3421 ccccgataaa gcgccaatta tcaaaaattt gaaagagaag aatattccca ttatttccga
     3481 aattgaattt gcagggcggt ttacaaatgc caaaatgatt tgtattaccg gcagtaatgg
     3541 gaaaacaacc acaaccatgc tgacttatca tattttgaaa aatgcaggtt acaatgttgg
     3601 tctggcaggc aacgtaggac aaagtcttgc ctatcaggta gcaacaagtt cgcgcgatta
     3661 ctatgtggta gaaatcagca gttttcagtt ggacgggatg tatgatttta aagcagatgt
     3721 agcaatattg ctcaacataa cacccgacca tttggaccgt tatgaatata attttcaaaa
     3781 ttatgtcgat tcaaaattcc gtatcatccg taaccaaacc tctgaagacg ctttcatttt
     3841 ttgggacgat gatccggtta ttcagtcaga aatagcaaaa cgctctatac tcgcagcaaa
     3901 atatcccttt tcactcaaaa agaaagagca ttgtaaagct tatatcgaaa acaacaacct
     3961 gatcatagaa acttctaaca acccattgat tatgccggta aaagaattgt cgttaaaagg
     4021 aatacacaac acctacaata tgatggctgc cggtttggcg gcatcggctg tgaatgtaaa
     4081 aaaagaattt attcgccagt cgttacagga ttttcaaggc gtggaacatc gcctcgaata
     4141 tgtagctaca gttaaaaacg taactttcat caacgactcg aaagcgacca atgtcaattc
     4201 atgctggtat gcgctggaaa gcatgaaaac acccgttatt ctgattttgg gcgggacgga
     4261 caaaggcaac gattattccg aaattgaaag tctggtgaaa gaaaaagtga aagcattggt
     4321 ttttttggga gtagacaaca cgcctttaca tcgttttttc gatggaaaaa tagataaaat
     4381 cgtggatgtc cgctcgatgg aagatgccgt aagagcagct tatgcaatgg ccgaagaagg
     4441 agataccgtt ttgctatcgc catgctgcgc cagctttgat ttatttcaga attacgaaga
     4501 cagaggaaga caattcaaaa cttgcgtaag gaatctgtga tttatcaatt gtatatgttt
     4561 atttaagcaa agagacagat ggaaaatttt ctgaaaaaat atttaaaagg aaatgcaaca
     4621 atttggattg tattcatttt gttgtgcctg gtttccattc ttgaaatgta cagtgcttcg
     4681 agcacactgg cattcaaatc ttccaatacg gccatgcccc ttttgcgcca tgccggtttt
     4741 ttgctctttg gggttatttt ggcatttctt gtccatctga ttccatacaa atatattcga
     4801 ttgctggctt atttgggact atttttttcg ttcatactcc tgattctggt ttattacaag
     4861 ggtcaatcgg caaacgacgc aacacgttgg tttgaaattg gcggactgca atttcagcct
     4921 tcagaactgg caaaactttc ggtcattatt gtagtggcgg attttatgtc gcgcatcaaa
     4981 aatgaagcag cagacgaaaa gaagtatttt cggtatattc tcattgtact ggctgcaata
     5041 tgtcctttga ttctttttga aaatttttct actgccatgc ttctttttgg tgtcgtattc
     5101 cttatgatgt ttattggagg aatatccaga aaaaaattgg gattattgct gctgtctatt
     5161 acactttttg tgttgggtgg gtacgctatg attaaaaccg ttcctgaaga ctcgatgccc
     5221 aaaattttcg accgtgccta tacatggtcg gcacgcatcg accgatacac cgaagaaaag
     5281 aaacaggaag aaaacaaata tgtcattacg gacgaaaacc ggcaggtgca acacggaaga
     5341 atagctattg cccgaggcgg aatgttggga gtaatgcccg gcaacagtgt ggaaagggat
     5401 tatcttcctc aagcttattc cgattttatt tacgccatta ttgtagaaga aatgggattg
     5461 gttggagggg taataatcat tctgttttat ttggttctac tttacagagc cggccgcatt
     5521 gccgtaaaaa gtgaaacggt atttcctgcc ttgctagtca taggactttc cctgatgata
     5581 gttttacagg cttttgtcag catggctgta gccacaagtt tgggccccgt taccggtcag
     5641 cctttgccgt tgataagccg aggtggaact tccatcatta tcacctgcat atattttgga
     5701 attattttag gcgtaactcg acaactgaaa ggagaacaac atgtcgaaga ggaaatacct
     5761 gaagaacagg agcgcaattt tcccgttgtg gatttgaacg aattatccta attttgtaaa
     5821 ttgtttttta tacaacgttt tagaaaatta ttgatgtaaa tttcagtttt actgacaatt
     5881 ataatttaaa acaatgcctt atagatttat catcagcggt ggaggaacag gaggtcatat
     5941 cttccctgcc atcagtattg ccaacgctct gaaaaagcag ctgcccaatg cagaaatact
     6001 ttttgtcgga gctttgggtc ggatggaaat ggaaagagtt cctgctgcag gttatcccat
     6061 caaaggttta cctgtaagcg gttttgaccg aaaaaacccg tttcgcaacg tcaaagtgct
     6121 gttgaatctt cggcgaagtt tacaaatggc tcgtcaaatc attcgcgatt tcaaacccga
     6181 tgcagttatt ggagttggag gatacgccag tgccccgacc ttgaaagctg cttccgattt
     6241 gggtattcca acactgattc aggagcagaa ttcgtatgcc ggcattacca acaaactgct
     6301 ggcaaaaaaa gctgcacgca tttgtgtggc ttacgaagga atggaacgat ttttccctaa
     6361 agaaaaactg gtattaaccg gcaatcccgt acgtcaggaa ctgctgtcaa tacccgaaaa
     6421 aagtaaagaa gcctttgaat tttttggatt tcgtccggaa aaaccaaccc ttctggtagt
     6481 cggaggaagt ttgggagcac gaactatcaa tcgcagcata atatcgcatt tgcaggaact
     6541 ttcccaagcc gatatccaaa ttatatggca aaccgggaag ttttatattg aagaagccag
     6601 gaaagctgcc gaaccatttt cttcgcctca attaattgtt acagattttg tttctcggat
     6661 ggatttggca tattccgtag ccgatttggt tatatcacgt gccggcgccg gttcaatttc
     6721 cgaattgtgt ctgctgggga agcccgctat tctggtacct tcacccaatg tggccgaaga
     6781 tcatcagaca aaaaatgcgc ttgcactggt tcagaaggaa gccgctgtga tgataaaaga
     6841 cgaagaggcc gaaaaaatgc tgataccgaa agcgttggag ttaataaaag atgcggactc
     6901 actcaataaa ctgggccaaa atatcaggaa gctggcgcag aaagattctg ccgaacgaat
     6961 tgcggcagaa gtattgcaac tgatttcaaa aaagaattga ttcataacaa atcgaatacg
     7021 acctaaacca attttaattt taaaaaatgg attttacaaa atattatttc ttgggtattg
     7081 gaggaatggg aatgagcgct ttggctcgct atttcaattc caaaggattt caaactgccg
     7141 gttatgaccg cgttcagtca aaagttactg ccgacttgca gacggaaggg atacaaatca
     7201 atttcgaccc caatgtggat caaattccca tctcgttcag atatcccgaa aacactctgg
     7261 tggtggttac tccggctatc cctgatgatc tgccacaact cgtttatttc cgagaaaacg
     7321 gatttaccat taaaaaaaga gctgaagtgc ttggcgacat tacccgtaaa gcttctacca
     7381 tttgtatagc cggaacacac ggcaaaacga caacttcaac tttgacagct catttgcttt
     7441 accagtcgca tatttcctgc aatgcttttt tgggcggaat tgccaataac tacaactcca
     7501 atcttttgtt atcgaaagat agcaatttgt tcgtaataga agctgatgaa tacgaccgtt
     7561 cctttcatca actctctcct tatatggcag ttatcacttc cgccgaccct gaccatttgg
     7621 atatctatca aacgcctgaa gcttatcgcg agagttttga gcatttcacg tcgctcatca
     7681 gcccaggtgg cgttttgttg atgaaaaagg gagtcaatat ccaaccgcgt ctgcaaaaag
     7741 gagtaaagca atatacctac tcaatgaatg acggaggaga cttttacgct gaaaatattc
     7801 atttggtgaa tcatgaattc catttcgatt ttgtaacacc ttccgaacgc atttcggata
     7861 tacggttaaa aattccaatt cgcatcaaca tcgaaaatag tgttgctgcc attgctttag
     7921 catggttgaa tggcgttaca aaagaagaaa tacgcacagc aatagcctct tattctggta
     7981 tttatcgaag atttaatctg gtttttcgtt cggaacaaac aattttcatg gacgattatg
     8041 cacatcaccc aaaagaattg aaagccagca ttttgtccat caaagaatta tttcccgaaa
     8101 agaaaattac gggaatattt caacctcatc tctatagtcg gacgcgtgat tttgcttccg
     8161 attttgctga agcattatca ttgctggatg agttgattct gctggatatt tatccggcaa
     8221 gagaagaacc cattgaagga ataagctctg aaatgatttt tcgtaatgtt aaactggaca
     8281 ataaaacact atgttccaga gaaaatctat tatctttgtt aaaggacaag catccggaag
     8341 ttttggttac ttttggagcc ggcgacatag ataagctggt tccgctcatt aaaaaacagc
     8401 tgatggagca aaccaatgga aatgcttgat gaacaatatc ttcccgtttt ctgaaagaaa
     8461 gaaaaaaatc gaaattgggt tgaaacgtat aagaaaatat atttttcaac aagtaggaag
     8521 cgttcttaaa ttatgaaact ggaataaagt catgaaacct gttttcaaat atatattgat
     8581 aacattcggt attctgctgg taacaggata tttggcttgg gcattgttga cttttcccga
     8641 cccaaaagca aacgaagttt gcaacaggtt ccacattgtt tttttggatc aatcaggcgt
     8701 taaacttatc tccgaaaaag aagttgccga aatgatcgat atcaaaggtt tgaaccctat
     8761 gggaaaaacg ctcaaacatg taaggacgga agaaatagaa gaattattga caaaacatcc
     8821 tttcatcaaa caagctgaat gtttcaaaac gccatcaggt ggagtgtatt tatttctgca
     8881 acagcgtcat ccaaaattca gactggcagg caatgaaaac ttctatgtgg acgacgaaaa
     8941 aaatataatt cctgtttcct ataacgatgc agcacgtctc cctgtagttt cgggattggt
     9001 tacaaaatcg atggcgaaag gcgaattgtt cgattttgtg tcttacttgg aagaaaatcc
     9061 tttttggaat gctcaaatcg aacaaattta tattcgcccc gacagaaaaa ttgaacttgt
     9121 tcctcgtgtt ggaaccggca tcattttgct gggcaattta aaagattatg agaaaaaact
     9181 ccacaaacta aaaatactct acgaaaaagg gttcaatgaa tttggttgga attattacaa
     9241 aacaattgat cttcagtacg aaaatcaaat tgtctgcaca aaaaccgaca atattgattg
     9301 atgcaatggc tgaagttttt tgatattttt ttaagataaa tatttatgaa tcagaacaca
     9361 tatatagcac ttgatttggg aagttcgcgt ataactgcta tggctgccga agtattggaa
     9421 agtggagcgg tgaaggtact tgccacagaa tcgaaagcag ccgatgatgt ttatcacggc
     9481 attatcgaac acacttcagg cgctgctttt aaaattagcg aacttttgaa acttttgcaa
     9541 aatagcgcaa aaattcgcga tattcaacaa gtttgtgtgg ctctgaatgc caaaagcatg
     9601 aaactttcca aatcttcgac cactcgtttt attggccagg gaaaaattgt tacagatgct
     9661 ttgctggatg aaatgaaaga agaatgtgaa cgaaaaaatt ccgatgaaaa caacatagtg
     9721 tttgatgtaa ttccggtttc attcgaaatt gacgggaaaa ttacaactga tcctgttgga
     9781 caatccggga atcagattac aggctattat catgttgtaa aaggaaatgc acgcattctg
     9841 cagcaattga aaagctgttt cgttcgcaat caaattcaga tcgaatatgc acctctttca
     9901 gtggaagctt tatctaccgt actgacagaa gaagaggaac ggcagaatgg atgtgcgctg
     9961 atagactttg gggcttccac tactacattg gccgtttaca agaatgatgt tttgcaggat
    10021 ttactggtcg ttccgctcgg aagcaagaac ataacctacg atattgcaga actcggcatt
    10081 gaagaatcaa atgccgaacg cctaaaatgt ttgacaggag tagcaatgga aaaatttgtc
    10141 gaaaagcatg tttatgttca ggttgctcct tcttccggca ctgatccggt aagaatttcc
    10201 acactttttc tggctcagat tatcaatgca agattggatg aaatgaccga acctgttttt
    10261 aaagtactgg aagagttgtc attcccactg gaatcgggaa ttatcataac cggcggaggc
    10321 gccaaactga aatttttaaa ggaatttgta gaagaaaaaa gcggtttgaa ggtacgttgg
    10381 ggcaatcact ccgactggtt ggaagatcaa gcacccgatt atttggccga ttcatcttat
    10441 tctcaactta ttggcactat tttactggca aacgaaaatc gacgtcttca tccgttaaaa
    10501 tcggaaaaca caacaaaaaa aggacaaaaa aacaagaaaa aaattacaga aagaattacc
    10561 gatggtttcc tcaggttttt cgatgatgaa accacttgga ataaatgaga aatttttaat
    10621 gaacattata aattgatatg atcaatatag aagaattagt tgaaatgttg cctttggaac
    10681 ttcccgaaat agaaaattcc atcatcaagg taata
//
LOCUS       DAOSCH010000071        10700 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4078_length_10700_cov_6.070925, whole genome shotgun sequence.
ACCESSION   DAOSCH010000071 DAOSCH010000000
VERSION     DAOSCH010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4078_length_10700_cov_6.070925"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(386..1141)
                     /gene="tpiA"
                     /locus_tag="PLZ54_06285"
     CDS             complement(386..1141)
                     /gene="tpiA"
                     /locus_tag="PLZ54_06285"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOJ66570.1"
                     /translation="MRKNIVAGNWKMNKTLPEGLALATELDKMLSNYQPKCEVIIAPP
                     FIHLASITATIDTKKIGVSAQNCADKESGAYTGEVSAAMVKSTGANYVILGHSERRTY
                     YGETPVLLKEKVNLALKNELTPIFCIGEVLAERESGIYFDVVKSQIEGSLFHLSAEDF
                     SKIILAYEPVWAIGTGKTATPQQAQEMHAFIRQIITEKYGKNIAENTSILYGGSCNPG
                     NAKELFANEDVDGGLIGGASLKAADFKAIIDAF"
     gene            complement(1223..2356)
                     /locus_tag="PLZ54_06290"
     CDS             complement(1223..2356)
                     /locus_tag="PLZ54_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOJ66571.1"
                     /translation="MAFPAEQKGKKILFVRICSILLGALFVFSGFVKAIDPLGTTYKI
                     EDYLNAFGGPFPQLVFLAFPAAVFLSTLELVMGLNLLLRIYVRFTTWVAAVFLFFMTL
                     LTFYIALKNPVTDCGCFGEAIVLTNWQTFIKNIFFIALSTFLLINRKHLYPLVLPSLE
                     AIFTVSFVIVGVGISVYSYFHLPIMDFRPYKVGVNIPEAMKIPEGAKPDVYETTFIYE
                     KEGKQKEFNLENYPKNDKSWKFVDQKTKLISKGYRPPIHDFTILNKDNEDISDIILSQ
                     PDSTFLLIMYDINKTSQEGAKKAERIFQKTQQNGHSFYAITASTDNDIQKFTERTKIS
                     FPFYKADPIMLKTVIRANPGLVLIKDGTILGKWNWRDFPENFY"
     gene            complement(2414..2980)
                     /locus_tag="PLZ54_06295"
     CDS             complement(2414..2980)
                     /locus_tag="PLZ54_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HOJ66572.1"
                     /translation="MNQTDKQFDQIAAICRNIFIQKLKDYGASWRLLRPTSVTDQLYI
                     KANRIRNIQVKGFSKINEGIRSEFIGIVNYAIIGLIQLEKGFAEKPDLSVEETIALYD
                     KYMQQAKELMMDKNHDYDEAWRHMRIESMVDLILMKIERTKQIEKNDGKTLISEGIDA
                     NYFDMINYAIFALIKMDENDSQQKNEKI"
     gene            complement(3283..3630)
                     /locus_tag="PLZ54_06300"
     CDS             complement(3283..3630)
                     /locus_tag="PLZ54_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HOJ66573.1"
                     /translation="MKKNKLSIQENQELIDFAVTARKYKTQLTKKFLNRKKWENPSPF
                     EIYSFIPGTVVDIFVNEGDIVEEETPLMTLEAMKMQNKIEMPFTAKIKKINVKKGEQI
                     PKDFLIFELEPVE"
     gene            complement(3831..5375)
                     /locus_tag="PLZ54_06305"
     CDS             complement(3831..5375)
                     /locus_tag="PLZ54_06305"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOJ66574.1"
                     /translation="MSAKKNIIALRQKKAIIEKGGGEKAIEKQLAMGKMTARDRIRAL
                     LDEGSFHEYDLFVQHEEKNFGMDTKDLPGDGVITGTGSINGNPVCIYAQDFTVMGGSL
                     GIQHARKITKIMDHALKMKMPCIGINDSGGARIQEGIGALAGYGEIFYRNTMASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVENISKMFITGPNVIETVLGEKISQEDLGGARV
                     HAEITGNAHFYALSEKECLQQIKELISYIPHNNEEKAARVEPKKPKFKKKIENIIPTN
                     PKLPYDVREIIYALSDDSKFFEVHQLWAPNIVVGFARIEGETVGFVANQPMYLAGVLD
                     CDASDKAARFIRFCDAFNIPLITLEDIPGYLPGVDQEHAGVIRHGAKVLYAYSEATVP
                     KITVILRKAYGGGYIAMNSRHLGADFTFAWPEAEIAVMGPEGAANIIFKKEIMEAEDP
                     DAMRQLKVKEYIEKFANPFVAAGKGYIDAVIEPSETRELLIHSLSVSRKKFDYRPTRK
                     HGNIPL"
     gene            complement(5541..5831)
                     /locus_tag="PLZ54_06310"
     CDS             complement(5541..5831)
                     /locus_tag="PLZ54_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOJ66575.1"
                     /translation="MRRKNTELLSEIIRQMLKDQHLEKPLYEKRIIDAWTSVLGPQIK
                     KYTGHLEVKNGILYVSINSAVLRHDLFLSREEIKNALNKEVGHEVINAIVFR"
     gene            complement(5854..6798)
                     /locus_tag="PLZ54_06315"
     CDS             complement(5854..6798)
                     /locus_tag="PLZ54_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOJ66576.1"
                     /translation="MYSVASKFRLFIVLLFATNIAFGQQLNKNYLSYIEKYHQLAIKQ
                     QKKHGIPASIILAQGLLESSAGQSQLSQLSNNHFGIKCNSDWNGETISWDDDEKNECF
                     RKYKNVADSYEDHSLFLKNRPRYAFLFQLDKTDYQGWAHGLKKAGYATDPTYAYKLIS
                     LIENYNLNRFDKQIFDNNYAERMEIQINTSIPQTIQKVSDYVTHKKFKNNGVPYVIAV
                     PCDTYANIAQEFNLSEETLRSYNEVSATTALQPGEKVYIRPKKNKASKNYPVHVVQAG
                     ESMHDIAQFYGIKTEKLYKMNHIPFTEGAQVGQVLKLR"
     gene            7073..7555
                     /locus_tag="PLZ54_06320"
     CDS             7073..7555
                     /locus_tag="PLZ54_06320"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOJ66577.1"
                     /translation="MFKKSTKVTYSVCRFDELTSLQQQLIDKAKEQVQKAYAPYSGFY
                     VGAALELENGEVIAASNQENSAYPSGLCAERVAVFYANAQYPDIPVKTLAIAAFTNGE
                     FLKMPVTPCGACRQVLLETELRFEKNITILLYGSQKIYIISCVKDLLPLSFDKLQLQK
                     "
     gene            7691..8074
                     /locus_tag="PLZ54_06325"
     CDS             7691..8074
                     /locus_tag="PLZ54_06325"
                     /inference="COORDINATES: protein motif:HMM:NF039344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="START-like domain-containing protein"
                     /protein_id="HOJ66578.1"
                     /translation="MQKSIFTLEYPLKSASANFLWTKIGSPLGLSEWFANEVDLEDSK
                     YVFKWEEFQQSARLLELKPNKSIRFQWLDDLDTPAYFEFRIDTQELTGEVTLVITDFA
                     WEDEKEDAILLWNYQVDELKRIAGM"
     gene            8241..9728
                     /locus_tag="PLZ54_06330"
     CDS             8241..9728
                     /locus_tag="PLZ54_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOJ66579.1"
                     /translation="MFKIKKIDIYVFKNFISLFIVTFLICIFILLMQFLWRHFEDLVG
                     KGIGWSVLFEFFFYSALSLVPMALPLAILLASLMTFGNLGETLELTAMKAAGVSLFQI
                     MRPLLFFISLICVAAFFFSNFALPMAQTKLWTLIFSLKQKSPELEIPVGEFYTGINGY
                     NIYVREKDGKLLKDLMIYDFSAGFDNAIVMVADSGEIQLSSDKKNLLLSLYSGESFEN
                     LKNQQIGSSPSSIPYRRESFRTKEIVVDFNAEFNRFDESLLNDQHISKNISQLSQSID
                     SVNKIVRSMEIQQSKEIMQHPYINWSINQPESTLLPEEKLSTYSFDSLFLSLNEESMF
                     RAIDLAISEVQQIKSSIDFNKIMLEQQIMYVRRHWIEWHRKFTLSFACLIFFFIGAPL
                     GAIIRKGGFGMPVVISVVMFIIYYIIDNTGYKMAREGIWSTFQGMWLSSAVLLPIGIF
                     LTYKAVTDSALFNSENYTRIWNSLVQKIKIWIDKFFALQLKKRHA"
     gene            9739..>10700
                     /gene="ribB"
                     /locus_tag="PLZ54_06335"
     CDS             9739..>10700
                     /gene="ribB"
                     /locus_tag="PLZ54_06335"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HOJ66580.1"
                     /translation="MMEIKLNTIEEAINDIRNGKFVIVVDDEDRENEGDFICAAEKVT
                     PEMVNFMITYGRGVVCAPLTEERCEELELEMQVENNTSMHSTPFTVSVDKLEGCTTGV
                     SAADRAATLRALADPSSTPETFGRPGHIFPLRAKSKGVLRRAGHTEAAVDLARLAGLY
                     PAGALVEIMNEDGTMARLPQLMEIAERFDLKIITIKDLIAYRLKFESLIEKGEMVNLP
                     TAYGNFKLIPFRQISNDKEHVALIKGEWEKDEPILVRVHSSCVTGDIFGSLRCECGEQ
                     LHKAMEMIEKEGKGVIVYMNQEGRGIGLMNKIKAYKLQEQGMDTV"
BASE COUNT         3110 a         1871 c         2062 g         3657 t
ORIGIN      
        1 gtaacgggca agtgcctcaa gctgttcgta agtggacata taaaaagtat tttttttcac
       61 aaagatactt ttctttttct acaaacacat gtttttgcag gtgagccgta tttgtagaat
      121 agccaatttt ataaccattc acattttcaa tataagaaac ccacatgtat tgcctacaca
      181 aacacgaatg aatataatat tcagacatgt gggttccatc agacctttaa aaataaatta
      241 tattctgatg aaaaattgaa ggtttattga attggactaa aacctgctaa cgtagagcct
      301 aaataccact cagtggaatt ttaccgaatt ccatttttgt agtatgacat gatcatattt
      361 ggtaagtttc gttaatgttc agcgcttaaa atgcatcaat tatggctttg aaatcggcag
      421 ctttcaaaga agctccacca atcagtccgc cgtccacatc ttcgttagca aacaattctt
      481 tagcattgcc cggattgcag ctgccaccgt aaagaatgga agtgttttcg gctatgtttt
      541 ttccatattt ttcggtgatg atttggcgta tgaaagcatg catttcttgt gcctgctgag
      601 gtgtggcagt tttcccggtt ccaattgccc aaacgggttc gtaagcaagg attatttttg
      661 aaaaatcttc tgcagaaagg tggaacaatg agccttcaat ttgagatttc actacgtcaa
      721 aataaattcc cgattctctt tcggccagta cttcaccaat gcaaaaaatg ggtgtaagtt
      781 cgtttttcaa tgccagattc actttttctt tcagtaggac gggagtttca ccataatatg
      841 ttctgcgttc ggaatgacct aaaataacat agttggcacc ggtagatttt accattgctg
      901 cactcacttc tcccgtatag gctcccgatt ccttatcggc acaattttgt gcagaaacgc
      961 caattttttt tgtatcaata gttgctgtta tgcttgccaa atggataaac ggcggagcaa
     1021 taattacttc acacttgggc tggtaattcg aaagcatttt gtccagttct gttgccaatg
     1081 ccaaaccttc cggtaaggtt ttattcattt tccaatttcc ggctacaatg ttttttctca
     1141 tatttactgt ttttttattg agtttgataa atttagttta tttgtagaag tttgtctgaa
     1201 gaaatttatt ctttttcgac gcttaataaa agttttctgg aaaatctcgc caattccatt
     1261 ttcccaaaat cgttccatct tttatcagta ccaaacccgg attggcgcga atgacggttt
     1321 tcagcatgat aggatcggct ttgtagaaag gaaatgaaat ttttgtacgt tctgtaaatt
     1381 tttgaatgtc gttatcggtt gaagctgtaa tggcataaaa cgaatgcccg ttttgctgag
     1441 ttttttgaaa aattctttct gcttttttcg ccccttcctg agaagttttg ttgatgtcgt
     1501 acatgatgag cagaaaagtt gaatcgggtt gagaaagaat aatgtcagaa atatcttcat
     1561 tgtctttatt gagaatagta aaatcgtgaa tcggaggtcg atagcctttc gaaatcagtt
     1621 tggttttttg gtctacaaat ttccagcttt tgtcgttttt cgggtagttt tccagattga
     1681 attccttttg tttcccctct ttttcgtaga tgaatgtagt ttcatatacg tcgggtttgg
     1741 ctccttccgg aattttcatg gcttccggaa tatttacacc cactttgtag ggacgaaaat
     1801 ccatgatagg caagtgaaaa taagaataaa ccgaaatgcc aacgcctact ataacgaatg
     1861 aaacagtgaa aatggcttct aatgaaggaa gaacaagcgg ataaagatgt ttgcggttga
     1921 taagcagaaa agtcgacaga gcaatgaaaa aaatattttt aataaaagtt tgccaattcg
     1981 taaggacaat tgcctcgcca aagcagccac agtccgtcac gggatttttt aaagcgatgt
     2041 aaaaagtcag caaggtcata aaaaacagga atacagcggc tacccatgtg gtgaatcgta
     2101 cataaattcg gagcaaaaga ttcagtccca tcaccaactc caaagtagaa agaaaaacag
     2161 cagccggaaa agccaaaaaa accaattggg gaaaaggacc gccaaaagca ttcaaataat
     2221 cttcaatttt ataagtagtg cccagcggat cgatggcttt tacgaaacct gaaaatacaa
     2281 aaagcgctcc caaaaggatg ctgcatattc gaacaaatag aatttttttc cccttttgtt
     2341 cggcaggaaa agccattttt ttgtttttat aaatatctga cgacatgaga tttaattaat
     2401 tgcttcagtg gctttaaatt ttttcgtttt tttgttgact gtcgttttca tccattttga
     2461 ttagggcgaa aatggcataa ttgatcatat caaaataatt ggcatcaatg ccttccgaaa
     2521 tcaaagtttt tccatcattt ttttcgattt gtttggttct ttcaattttc atcaatatca
     2581 aatctaccat cgattcgatt cgcatgtgtc tccatgcttc gtcgtaatca tgatttttgt
     2641 ccatcattag ttctttagcc tgttgcatgt atttgtcata cagagcaatc gtttcttcga
     2701 cggaaaggtc aggtttttcg gcaaatcctt tttccaattg aatcaaaccg atgattgcgt
     2761 aatttacaat gccgataaat tcggatcgaa tgccttcatt gattttggaa aaacctttta
     2821 cctgaatgtt cctgatgcgg ttggctttga tatagagctg atcggtaacc gaagttggac
     2881 gcaacaaacg ccatgaagcg ccataatctt tcagcttctg aatgaaaatg ttgcggcaga
     2941 tggcagcaat ctggtcaaat tgtttatcgg tctgattcat aggtaaaagt tggtttttca
     3001 gttaatgaaa attttgggtt ttcccaatca acagttggat aagaaaatac ttccgttgag
     3061 agtacaaaag tataatattt tatgaagata ggcaatttga agttttgatg aaaagcaaac
     3121 ttttgggcaa gcgagggctg aaagttgttt ttttaattgt tattcaattg tttctaccac
     3181 aatatgtaaa aaaatctctc cgcttttttc gacattgttg tcgttattga cgaagagatt
     3241 tttttcggtt taaacacagg atgttttttc ctgaaattgc cgttattcga ccggttccaa
     3301 ttcgaatatc agaaagtctt taggtatttg ctcacctttc ttcacattaa ttttttttat
     3361 ttttgcagtg aaaggcattt ctattttatt ttgcattttc atggcttcca gcgtcattaa
     3421 gggtgtttct tcttccacaa tatcgccttc gttaacgaaa atgtctacca ctgttcccgg
     3481 aataaatgaa tagatttcaa aaggactcgg attttcccat ttttttctgt tcaagaattt
     3541 tttggttagt tgagttttat attttcgcgc agtaactgca aaatctatta attcttggtt
     3601 ttcctgtata gataatttat tttttttcat aaatactatg tttttaagtt gagcaaaaag
     3661 ctcgttaatc agctttttgg cataagtcgg cttttttcaa ataatttttt ggttcttttt
     3721 actgaaagat tatcaagaca aacggttgtt ttttatcata attttgataa ttaattagtt
     3781 gcagaaacaa aaaataaaga tgaaaaatac tgatttttcg ccgacccaaa ttataaagga
     3841 atatttccgt gttttcttgt cgggcgataa tcaaattttt tccttgatac acttagcgaa
     3901 tgtataagca gttcacgagt ttccgaaggt tcgataacgg catcaatata acctttaccg
     3961 gcggcaacaa atggatttgc aaatttttcg atgtattcct taacttttaa ttggcgcatg
     4021 gcatcgggat cttcagcctc catgatttct tttttgaaaa taatgttggc agcaccttcc
     4081 ggtcccataa ctgcaatttc ggcttcaggc caagcaaaag taaaatcggc gcccaaatgt
     4141 ctggaattca tggcaatgta tcctcctcca taagcttttc tcaaaatgac ggtgattttt
     4201 ggaacagttg cttccgaata agcataaagc acttttgctc catgacgaat aacgcctgca
     4261 tgttcctgat ctacacccgg caaatagccc ggaatatctt ccaaagtaat gagcggtatg
     4321 ttaaaagcat cgcagaatcg aataaaacgg gcagctttat ctgaagcgtc gcaatcgagt
     4381 acaccggcca aatacattgg ctgattggca acaaaaccaa ccgtttctcc ttctatacga
     4441 gcaaatccca ctacaatatt gggtgcccaa agttgatgta cttcaaaaaa tttagaatca
     4501 tctgaaagcg cgtaaattat ttctcgtaca tcgtatggta attttggatt tgtcggaatg
     4561 atattttcaa tttttttctt gaattttggc tttttgggtt caactctggc agctttttct
     4621 tcgttgttgt gaggaatgta agaaataagt tccttgattt gttgcagaca ttccttttca
     4681 ctcagtgcgt aaaagtgagc attacctgtg atttcggcat gtactctggc gcctcccaaa
     4741 tcttcctgtg aaattttttc tcccagtacg gtttcaatca cattaggacc cgtgataaac
     4801 atttttgaaa tattttctac gacaaacaca aaatcggtca gtgccggaga ataaactgca
     4861 ccaccggcgc aaggtccaag aataactgaa atttgaggaa ttacaccgga agccatagtg
     4921 ttgcgataaa aaatttcgcc ataaccggcc aatgcgccaa ttccttcctg aatacgtgca
     4981 cctcccgagt cattgatgcc gatgcacggc attttcattt tcagcgcatg atccatgatt
     5041 tttgtgattt ttctggcatg ttgaattcct aaagaaccgc ccataaccgt aaaatcttga
     5101 gcataaatgc aaacaggatt tccgttgata gatccagttc ctgtaataac tccatcaccg
     5161 ggcaaatctt ttgtgtccat tccgaagttt ttttcttcgt gctgcacaaa caaatcgtat
     5221 tcgtgaaatg agccttcgtc caacaaagcg cggatgcggt cgcgggctgt cattttaccc
     5281 attgccaatt gtttttcaat agctttttca ccgccgcctt tttctatgat tgctttcttt
     5341 tggcgcaatg caataatgtt tttcttagct gacataacaa taaattattg tgattaaaca
     5401 taaaaattta ccctttaatg ggctttgaat aagtgagcaa aagtaacact ttttttcatt
     5461 gtatgttact tttttgaagt tttcttatgg aaggataaaa ttatatcgtt tatattcagt
     5521 atgttataga gaaatattta tcaacggaaa acgatagcgt tgataacttc atgcccaact
     5581 tctttgttta aagcgttttt tatttcttct cttgagagga ataaatcgtg tcgcaaaacg
     5641 gcagagttga tgctgacata taaaattcca tttttgactt ccaaatggcc ggtatatttt
     5701 tttatttgag ggcccaaaac agaagtccat gcgtcaataa ttcttttttc atacaacggt
     5761 ttttccaaat gttggtcttt caacatttgc cgaatgattt cgcttaataa ttccgtgttt
     5821 tttcttcgca tgattttaaa taaagagcat tttttagcgt aatttcagta cttgtcctac
     5881 ttgtgctcct tcggtaaatg gaatatggtt cattttataa agtttttcgg tttttatacc
     5941 gtaaaattgt gcaatgtcgt gcatggattc tccggcttgt accacatgaa ccggataatt
     6001 tttggaagct ttgttctttt taggccgaat atatactttt tctcccggtt gtaatgctgt
     6061 ggttgcgctg acttcattgt aacttctgag ggtttcttcg cttaaattga attcttgagc
     6121 aatattggca taagtatcgc acggaacagc tattacataa gggactccat tgtttttaaa
     6181 ctttttgtgg gttacgtaat cgctcacttt ttgaatggtt tgtggaatgg aagtgttgat
     6241 ttgaatttcc attctttcgg cataattgtt atcaaaaatt tgtttgtcga atctattcag
     6301 gttgtagttc tcaatcaggg aaattaattt gtaggcataa gtaggatcag tagcataacc
     6361 ggcttttttc agtccatgag cccaaccttg ataatcggtt ttgtctagtt ggaaaaggaa
     6421 agcatagcga ggacgatttt ttagaaacaa tgagtgatct tcatacgaat cggcaacatt
     6481 tttatatttt ctgaaacatt catttttttc gtcatcgtcc catgaaatgg tttctccatt
     6541 ccaatccgaa ttgcatttga tcccgaaatg gttgttcgaa agttgtgata gttggctttg
     6601 tccggccgac gattccagca aaccctgagc caggataatg ctggcgggaa tgccgtgttt
     6661 tttttgttgt ttgatagcca actgatggta tttttcgatg taggacagat aatttttgtt
     6721 gagttgctgg ccaaaagcaa tgtttgtagc aaaaagcaaa acaatgaaaa gacgaaattt
     6781 acttgcaact gaatacatga atgctatttt ctgaaatgat ttaaaaaaaa aaaattagtt
     6841 ggtcaatctc tgtcaaacta aatatgctcc acaggaatgt tgtttgaaaa acatattaga
     6901 aaaattcccg tagttttcag cttgatagat taaaccggct aataaagttt agtttaattg
     6961 caaaaatata atttattttc tcaaaaatga gcgttttgtt gattattttt ttacttttgt
     7021 accccctgct aaaggaaaat tttttgaaag tatcattaaa tcagattcta ctatgtttaa
     7081 aaaatcaaca aaagttactt attcggtctg tcgttttgat gaattgactt cattgcagca
     7141 acaattgata gataaagcca aagaacaggt acaaaaagct tatgcacctt attccggatt
     7201 ttatgttggt gcagctcttg agctcgaaaa cggtgaagtt attgcggcca gcaatcagga
     7261 aaattcggct tatccttcgg gtttatgtgc cgaaagagta gctgtatttt atgccaatgc
     7321 ccaatatccc gacatacctg tgaaaacgct tgctattgcg gcttttacaa acggagaatt
     7381 tttaaaaatg cccgttacgc cttgcggagc atgtcggcag gttttgctcg aaacagaatt
     7441 gcgttttgaa aaaaatatta ccattcttct ttatggaagt cagaaaattt atattatctc
     7501 ttgcgtgaaa gatttgttgc ctttgagttt cgacaagttg cagctacaaa aatgagtacc
     7561 acaatttttg ctgaattagt gagtgaaaaa ttttgaatgc tcgttttaat tcattatatt
     7621 gcagtcgtta atgtaataat gttttttagg aatttgagag ttagaataag aacgaaaaaa
     7681 taagagaaaa atgcaaaaat cgatttttac attggaatat cctctcaagt cggcttccgc
     7741 aaactttttg tggactaaaa ttggttcgcc gcttggctta tcggaatggt ttgccaatga
     7801 agttgatttg gaagattcaa aatacgtttt taaatgggaa gaatttcagc aaagtgcacg
     7861 acttttggaa ctgaagccca ataaaagtat ccgttttcaa tggctcgacg atttggatac
     7921 tcctgcttat tttgaattta gaatcgatac gcaagagctc accggtgaag ttacattggt
     7981 tattaccgat tttgcatggg aagacgaaaa ggaagacgct atcctgttgt ggaactatca
     8041 agttgacgaa ttgaaacgta ttgccggtat gtaaaagctg ctgttgcttg ttcttcaaaa
     8101 attagccatt tgtttgtccg acaagtggtt tttttatgct tttttttgct gataagtttc
     8161 attttttaag catatttcat gctacgaata atgtaaaatc aaacttttta gcgtaatttt
     8221 gtaattttaa gaaaacgaat atgtttaaga taaaaaagat tgatatatac gtatttaaga
     8281 actttatttc gcttttcatc gttacttttc tcatctgcat ttttattctt ttaatgcagt
     8341 ttttgtggcg tcattttgaa gatttggtcg gaaaaggaat agggtggtcg gtattatttg
     8401 aatttttttt ctactccgct ctttctcttg ttcctatggc cttaccactt gctattttgt
     8461 tagcttccct tatgactttt ggtaatctgg gagaaacttt agaacttaca gccatgaaag
     8521 cagcaggcgt atctcttttt caaattatgc gtccgttgtt attttttatc tcgttaattt
     8581 gtgtggccgc tttctttttc tccaattttg ctttaccaat ggctcaaaca aagctttgga
     8641 cattgatttt ttcactgaaa caaaaatctc ctgagttgga aattcctgtt ggcgaatttt
     8701 atacaggcat caatggttat aatatttatg ttcgagaaaa agatgggaaa ttattgaaag
     8761 atttgatgat ttatgatttt tctgcaggtt tcgataatgc catagtaatg gtggcagatt
     8821 cgggtgagat acagctgtca tcagacaaaa aaaacttatt gctttccctt tacagtggag
     8881 aaagttttga aaatcttaaa aatcagcaaa ttggcagttc gccttccagc atcccttacc
     8941 gacgtgaatc ttttcgaaca aaagaaatag tggttgattt taatgccgaa ttcaatcgtt
     9001 ttgatgaatc tttgttgaat gaccagcata ttagcaaaaa tatttcacag ctttctcaat
     9061 ctattgattc tgtcaataag attgtgcggt caatggaaat tcaacaatca aaagaaatta
     9121 tgcagcatcc ctatatcaat tggagcatca atcagcctga atctactttg ctgcccgagg
     9181 aaaagctttc aacttattct tttgattctc tttttttgtc gctaaacgag gaatcaatgt
     9241 ttcgtgccat tgatttggca atttctgaag ttcagcaaat caaaagcagt attgatttta
     9301 ataaaattat gctcgaacag caaattatgt atgtacgccg acattggatt gaatggcatc
     9361 ggaaatttac tctttccttt gcctgtctga ttttcttttt tattggagca cccttgggag
     9421 ccattattcg taaaggagga tttggcatgc cggtagttat ttcggttgtc atgtttatta
     9481 tttattacat tatcgacaat accggataca aaatggccag agaaggaatt tggtcaactt
     9541 ttcaagggat gtggctgagt tcggctgtat tgctgcccat tggcattttt ctgacatata
     9601 aggcagtaac agattctgct ctttttaatt cagaaaatta cacaagaatt tggaattctt
     9661 tggtgcaaaa aatcaagata tggatagata agttttttgc actgcaatta aaaaaaagac
     9721 atgcttaaat ttttataaat tatggaaata aaattgaaca ctatagaaga agccattaat
     9781 gacattcgta atggcaaatt tgttattgtg gtggatgatg aagatcgcga aaacgaagga
     9841 gattttattt gcgctgccga aaaggttacg cccgaaatgg tcaatttcat gattacttat
     9901 ggtcgtggag tagtttgtgc tcccctgacc gaagaaagat gtgaggaatt ggaactcgaa
     9961 atgcaggtag aaaacaacac ttccatgcat tcaacacctt ttacagtaag cgtagacaaa
    10021 ttggaaggat gcactacagg cgtttcggcg gctgatagag cagctacttt acgtgcactt
    10081 gccgatcctt catcaactcc cgaaactttt ggccgtcccg gtcatatttt tccacttcga
    10141 gcaaaatcaa aaggcgtatt gcgcagggct ggtcacaccg aagctgctgt tgatcttgca
    10201 cgtttagccg gtctttatcc ggctggagct ttggtagaaa ttatgaacga agatggaact
    10261 atggctcgtt taccgcaatt gatggaaatt gcagaacgtt tcgatttaaa aattataacc
    10321 attaaggatt tgatcgctta tcggttgaag tttgaatctt tgatagaaaa aggcgaaatg
    10381 gtcaatttgc ctactgcata cggtaatttt aaactgattc cttttcgtca gatttccaat
    10441 gataaggaac atgttgcctt aatcaaggga gaatgggaaa aagatgagcc cattttggtg
    10501 cgtgttcatt cctcttgtgt aacgggagat atttttggtt cgcttcgatg tgaatgtggc
    10561 gagcagttgc acaaagctat ggaaatgatt gaaaaggagg gcaagggcgt gatcgtttac
    10621 atgaatcagg aaggacgagg tattggattg atgaataaaa taaaggctta caagctgcag
    10681 gagcagggca tggatacggt
//
LOCUS       DAOSCH010000072        10492 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4193_length_10492_cov_5.946153, whole genome shotgun sequence.
ACCESSION   DAOSCH010000072 DAOSCH010000000
VERSION     DAOSCH010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10492
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4193_length_10492_cov_5.946153"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            132..695
                     /locus_tag="PLZ54_06340"
     CDS             132..695
                     /locus_tag="PLZ54_06340"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOJ66581.1"
                     /translation="MTIFDETKEQKKEIRNGIRELIRQISDTQKEKQAKSVFLKIEIL
                     PAFVQAKNILLYWSLPDELPTHTVVEKWATDKQILLPSVKGENFVIKKYSGLHALKKG
                     AFGIEEPLEIFDWHSPIELVIVPGVAFDKNKNRLGRGKGYYDRFLKSIDALKIGVGFD
                     CQLIEKIPVGKFDVPMNKVVVPSLIID"
     gene            complement(703..1428)
                     /locus_tag="PLZ54_06345"
     CDS             complement(703..1428)
                     /locus_tag="PLZ54_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66582.1"
                     /translation="MPYRRLPNTDQSRLRALRIALEKLENQDFTHQIVNFKTIHEAES
                     ILGVFENQMQQYRQALKNQVEANKRYQKLVGNVRMYISHFIQVLNLSVIRGDIKKEHK
                     KLYQLDPEVNNVPDLTSEAALLHWGKCIIDGENERLRLGGFPIYNPTIAKVKVHYEIF
                     KENKLNQKLYQSNTSRTLEDLGNLRIKVDEIIIDIWNQVEANFKDEKPYSKIQKCKEF
                     GLIYYYRKGEKKLLPEDAEEQKK"
     gene            complement(1480..2448)
                     /locus_tag="PLZ54_06350"
     CDS             complement(1480..2448)
                     /locus_tag="PLZ54_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOJ66583.1"
                     /translation="MIPFSEITKIINTELNKLNWDKKPEGLYRPIAYVLSLGGKRIRP
                     SLVLMACNLFSDKVESAIAPALGIEIFHNFTLLHDDIMDKAELRRGQPTVHKKWDENT
                     AILSGDLMQIAAYQLIGKVDELHLKKVLDIFSETAVEVCEGQQYDMEFEHREDVSPEE
                     YLEMIRLKTAVLLGAALKIGAIVGNANSKDANLLYEFGINLGMAFQLKDDLLDVYGDE
                     QFGKKIGGDIVANKKTYLLISAQKLAKGADAKELEKWLKTDSENETKVEAITALYNRL
                     EVRDICENKMQEFFEKSIDCLNRISVEAAKKIELRHLAEQLMSRKE"
     gene            complement(2456..3451)
                     /locus_tag="PLZ54_06355"
     CDS             complement(2456..3451)
                     /locus_tag="PLZ54_06355"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOJ66584.1"
                     /translation="MKKNIAIVAGGDSSEVVVSLKSAEGLHSFMDKQRFNVWIVTIIG
                     KTWQVECSDNEKYPIDRNDFSFTRKGEKIVFDFAYITIHGTPGENGILQGYFNLIKLP
                     FSCCDVLAAAITFNKFTCNQFLKNFDVKTAASVLLRKNEEIDLHEISAKTGFPCFVKP
                     NAGGSSFGVSKVKSIDQLEPAIRKAFSEDDEVVVESFLAGTEVTCGMYKTKAKRVVFP
                     LTEVVSQNEFFDFAAKYNGQVQEITPARISGSLTEKVQCQTARIYDILGCKGLVRMDY
                     IISEKEEIFLLEVNTTPGMTATSFIPQQIKAAGLNITDVLTEIIENELNYPTLIK"
     gene            complement(3543..4616)
                     /locus_tag="PLZ54_06360"
     CDS             complement(3543..4616)
                     /locus_tag="PLZ54_06360"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ66585.1"
                     /translation="MLEVTKWEDFSDSAEEPELFEHFRFIADKGQSPIRIDKYLVNCM
                     ANVSRNRIQEAADAGNILVNDKPVKANYKVKPNDVISIVMTYPPSQFEIIPQNIPIDI
                     VYEDDDLMVINKQPGLVVHPGVGNYEGTLLNALAYHFKDNPKFDFNDSRIGLVHRIDK
                     DTSGLLLIAKTEEAKTHLGKQFYNKTTERRYQALVWGNMKEDEGTIVGALARDPRDRM
                     MFKVFDEGENPLAKHAVTHYKVLERLTYVTLVECRLETGRTHQIRVHMKHIGHPLFND
                     ERYGGNEILRGTTSAKYRQFIKNCFDICPRQALHAKTLGFVHPTTGKQMNFDSELPED
                     MQQLLTKWRNYNTGSAAVEKEDF"
     gene            complement(4643..5482)
                     /locus_tag="PLZ54_06365"
     CDS             complement(4643..5482)
                     /locus_tag="PLZ54_06365"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOJ66586.1"
                     /translation="MDFKKFWKESFAGFVVKNLLLAIAIFLALTWITLFIIDIYTHHG
                     EAEIVPDMKGLYVEEAENILREKGLYPKIIDSVYVRDKKAGIIIEQVPPPKSTLKKNR
                     PVYLIINSTQVQKVPVPEVIDVSSRQAIAMLQSVGLDISKIEYKPSEFKDLVIDIKYN
                     GVSILPGTKIPESSSVVLVVGSGYGEGMFSVPSLKGMGLQEAKQAALSNSFVIGMVNF
                     DIPPKGNDADYFIYRQLPKAGTSMPAGSRIDIWLTSSPTKLLEDKEEDQIIILPENDE
                     VFF"
     gene            complement(5658..5816)
                     /gene="rpmH"
                     /locus_tag="PLZ54_06370"
     CDS             complement(5658..5816)
                     /gene="rpmH"
                     /locus_tag="PLZ54_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOJ66587.1"
                     /translation="MKRTFQPSQRKRRNKHGFRERMTTANGRRVLASRRAKGRKKLTV
                     SDEGRPKL"
     gene            6251..7798
                     /locus_tag="PLZ54_06375"
     CDS             6251..7798
                     /locus_tag="PLZ54_06375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOJ66588.1"
                     /translation="MKKRTIWILIILMVVTIVGLVLVQIRYVRINAEMIENQFKENVQ
                     RSLFQAVRLVEENEALQYLSETLEDVDYSAKSKGLIKSGNAQKDVLGMTTDSMAMDSG
                     KDFTLNQKLPSIQLSTKHGKATIEETSRYLQKKFQQDFSRSKTILDQAVFRWLRESDQ
                     KEISQRVNFDELNDILQTLLENNGVTLPFNYSVVDKQGRLVYKHTNISVDEQKISQTG
                     NVFTQKLFPFEETNQPYYLQVDFPTRQNYILNSMNLLLPSVVLVILILTIFIIAIIII
                     FRQKQLNNIKNDFVNNMTHELKTPIATISLASQMLQDPNIGKTPEMLQHVSKVIHDET
                     QRLSFQVEKVLQMAIFEKEKSPLNLTEIHINSLIQDVIHSFSLRVTNSGGKIIPSLKA
                     EKDLALIDEVHFTNVIFNLMDNALKYSNKPLLLNIETWNEKDRIFISIEDNGIGIRKE
                     DLKRIFEKFYRVSTGNLHNVKGFGLGLAYVKKIITDHKGTIKVESEPNIGTKFTISLP
                     ILKNQEK"
     gene            7795..8505
                     /locus_tag="PLZ54_06380"
     CDS             7795..8505
                     /locus_tag="PLZ54_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOJ66589.1"
                     /translation="MTDEKIKILLCEDDENLGMILREYLQSKGYDVDLLPDGEAGYKA
                     FTHKKYDLCILDVMMPKKDGFTLAADIRALNSEVPIVFLTAKSMKEDILQGFKLGADD
                     YLSKPFSMEELLYRIESIMRRIRGEKAREVTVYQIGNYTFDSQKQLITINGETKKLTT
                     KESELLSLLAANMNNVLERNYALKTIWVDDNYFNARSMDVYITKLRKLLKDDPNVAII
                     NIHGKGYKLIAPAVKEKN"
     gene            8737..10179
                     /locus_tag="PLZ54_06385"
     CDS             8737..10179
                     /locus_tag="PLZ54_06385"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOJ66590.1"
                     /translation="MKLRNFILTVLLFSFLLTYAEIPAGYYNSADGKNASALRTALKT
                     IITNGHSVTSYAGLWTAYKTTDVNANGKIWDMYSNCSFTYGTDQCGSYSNECDCYNRE
                     HTTPQSWFNEAPPMVSDLFNVYPTDGKVNGMRSNYPYGEVGSATYTSGNGSKLGSSSF
                     SGYSGTVFEPIDEYKGDFARTYFYMATRYADECGTWTGEATAVYSSSNGLTEYGKNLF
                     LKWSREDPVSQKEIDRNNAVYAIQNNRNPFIDYPGLEEYIWGTKTSEVFYVNASVSKP
                     SVSSPTATNVTYNSAVLGGTVTSTGGGTITETGVYYSTTDGFADGTGTKVSTSSPSTS
                     TFTVSINGLNSSTTYYFKAFATNSAGTGYSSQSSFKTSTLSSGNDPEIILGNPVATGS
                     TLNFGKVTANSTKSLLIKTKNLTGDLSVSVTGEMFNVSTSTISKSNAEEGYLLNVNYQ
                     PTATGSHTGTLTISGGGLTPDYVVQLAGSK"
BASE COUNT         3332 a         1993 c         1875 g         3292 t
ORIGIN      
        1 tttttctatc aaagctttgc aagattattt ggtgcaaatt taaaatcgga gttcgattat
       61 aacaaaaaag ataaaattct ggatagaatt atcgttctgg tggaagccgg gcaactataa
      121 atgaccattt aatgaccatt tttgacgaga caaaggaaca aaaaaaggaa attcggaatg
      181 ggattcggga attaattcgg caaataagcg acacgcaaaa agaaaaacaa gccaagtcgg
      241 tttttttgaa aatcgaaata ctccctgcct ttgtacaggc aaaaaacata cttttatatt
      301 ggtcgctgcc cgatgaactt ccaactcata ccgttgtaga aaaatgggct acagacaagc
      361 aaatcttact tccttcagta aaaggagaaa atttcgtgat aaaaaaatat tccggccttc
      421 acgcactgaa aaaaggtgct ttcggcatcg aagaaccatt ggaaattttt gattggcatt
      481 ctcccatcga gttggttatt gttcccggtg tggctttcga caaaaacaaa aatagattgg
      541 gaagaggaaa agggtattac gaccgttttt tgaaaagtat agatgcgcta aaaataggtg
      601 tcggtttcga ttgtcaatta atagaaaaaa ttccggtcgg aaaatttgac gtgccaatga
      661 ataaagttgt tgtaccttct ttgattatcg actaagatac gtttattttt tttgttcttc
      721 cgcatcttct ggcaaaagtt ttttttctcc cttgcgataa tagtaaatca gtccaaattc
      781 cttgcatttt tgaatttttg aataaggctt ctcgtccttg aaattggcct ccacctgatt
      841 ccagatgtct ataataattt cgtccacttt tattcttaaa tttcccaaat cttcgagtgt
      901 ccgcgaagta ttgctctgat aaagtttctg attgagcttg ttttccttga aaatttcgta
      961 atgaacctta actttggcaa tggtaggatt ataaatggga aaaccaccca agcgcaacct
     1021 ctcattttct ccgtcaatta tacatttacc ccaatgcagc aaagcggctt cgctggtcaa
     1081 atctggaaca ttgtttactt caggatcaag ctgataaagt tttttatgtt cctttttaat
     1141 atcgccacga attacagaaa gatttaaaac ctgaataaaa tgagaaatat acattcttac
     1201 attgccaacc agtttttggt aacgtttatt ggcttcaacc tgatttttta atgcttgtcg
     1261 gtattgttgc atttgatttt caaaaacgcc caaaatgctt tccgcctcat gaattgtttt
     1321 aaaattgaca atctgatgcg taaaatcctg attttcgagc ttttctaatg caattcgtaa
     1381 agcccgaagt cgtgattggt ccgtatttgg taatcttctg tatggcatgt ttttttcaaa
     1441 aaaaaattct tgaaatttat cgatgtcaaa gttaaaaaat tattccttgc gagacattaa
     1501 ctgctctgcc aaatgcctta attcgatttt ctttgctgct tcaacagaaa tcctgttcaa
     1561 acaatcaatg gatttttcaa aaaattcctg cattttattt tcacaaatgt ctcttacttc
     1621 gagtcggttg taaagagcgg tgatagcttc tacttttgtc tcattttcag aatcggtttt
     1681 caaccacttt tccaactctt tggcatctgc tccttttgcc agtttttggg cagaaatcaa
     1741 caaataggtt tttttgttgg ctacaatatc tccaccaatt tttttcccaa attgctcatc
     1801 gccataaaca tcgagcaagt catccttcag ctgaaaagcc attcccaagt tgattccaaa
     1861 ttcataaagc aaattggcat ccttactgtt tgcattgcca acaatagccc caattttcaa
     1921 tgcagctcca agtaatacag ctgttttaag acgaatcatt tccagatatt cttcaggtga
     1981 tacatcttca cgatgttcaa attccatgtc gtattgttgc ccttcacaaa cttcgacagc
     2041 tgtttcggaa aatatatcca gcaccttttt taaatgcaat tcatcaactt tcccaatcaa
     2101 ttggtaagca gctatttgca ttaaatctcc ggaaagaatt gcggtatttt catcccattt
     2161 tttgtgaacc gtcggctgcc cgcgtctcag ttccgctttg tccataatgt cgtcgtgtaa
     2221 cagcgtgaaa ttatgaaaaa tttcaattcc caaagccgga gcaatagcac tttccacctt
     2281 gtcggaaaaa aggttgcagg ccatcagaac cagcgaagga cgaattcgct tgcctccaag
     2341 tgaaagcaca taggcaatgg gacgataaag tccttcaggt tttttgtccc aattcaactt
     2401 attcagttct gtattaataa tttttgttat ttcactgaaa ggaatcatta taaatttatt
     2461 taattaaagt agggtaattc aattcatttt caataatttc tgtcagcaca tccgtaatat
     2521 tcaaaccggc ggcttttatc tgttgcggaa taaagctggt ggcagtcatt ccgggggtgg
     2581 tgttaacttc cagaaggaaa atctcctcct tttcggaaat aatgtaatcc attctgacaa
     2641 gtcctttaca acctaaaata tcgtaaattc tggcagtctg gcactgaact ttttccgtta
     2701 gtgagccact gatgcgtgcc ggcgtaattt cctgaacctg accattatat ttggccgcaa
     2761 aatcaaagaa ttcattctgg cttaccactt cggttaacgg gaaaacgact cgttttgctt
     2821 ttgttttata cattccgcaa gttacctccg taccggcaag gaaactttct accaccactt
     2881 catcgtcttc agaaaaagct ttacgaatag ccggttccaa ttggtcaata cttttcacct
     2941 tgctaacgcc aaaactgctg cctccagcat tgggcttaac aaaacaagga aatcctgttt
     3001 ttgcagatat ttcgtgcagg tcaatttctt cattttttcg caacaaaaca gaagctgccg
     3061 ttttaacatc aaaattttta agaaactgat tgcaggtaaa tttgttgaaa gttatggcag
     3121 ccgctaacac atcgcaacag gaaaaaggca acttaatcag attgaaataa ccctgcaaaa
     3181 tgccattttc acccggtgtt ccgtgtatgg taatataggc aaagtcaaat acaatttttt
     3241 ctccctttcg agtgaaactg aagtcgttcc ggtcaatcgg atatttttcg ttatcagagc
     3301 actccacttg ccaagttttg ccaataatcg taacaatcca tacgttgaat cgctgtttat
     3361 ccataaatga atgcaatcct tcagcgcttt ttaacgatac cactacttct gaagaatctc
     3421 cacctgcaac aattgctata ttttttttca tccaaaaaaa taaaaaatta agtatttatt
     3481 tgtcttcttt actgaaaaat cacagttaca tttgcctaat gaaaaataaa ctttggaaat
     3541 aactaaaaat cttctttctc cactgctgcc gacccggtat tataatttct ccattttgtg
     3601 agcaactgct gcatatcttc aggcagttcg ctatcgaaat tcatttgttt tccggtagta
     3661 ggatgaacaa atcccaatgt tttggcatgt aatgcctgac ggggacaaat atcgaagcaa
     3721 tttttaataa actgcctata tttggcagaa gttgtacctc ttaaaatttc atttccacca
     3781 taacgctcgt cattaaacaa aggatgacca atgtgcttca tgtgaacacg aatctgatgc
     3841 gtacgccctg tttcgagtcg gcattctacc aatgttacat aagtcaggcg ttccaacact
     3901 ttataatgtg taacggcatg ttttgccaac ggattttcac cttcatcaaa aaccttaaac
     3961 atcatgcgat ctcgtggatc gcgggccaat gctcccacaa ttgtcccttc atcttctttc
     4021 atgtttcccc aaacgagtgc ctgataacga cgttcggtag ttttattgta aaactgtttt
     4081 cccaaatgag ttttagcttc ttccgttttg gctatcagca atagtccgga ggtgtccttg
     4141 tcgatacggt gaaccagccc gattcgcgaa tcgttgaaat cgaactttgg attatcctta
     4201 aaatgataag ccagcgcatt cagcaaagtc ccctcgtaat tgccaacacc cggatgaacc
     4261 actaaacccg gctgcttgtt gataaccata aggtcatcat cttcataaac aatgtcaatg
     4321 ggaatgtttt gcggaataat ttcaaactga cttggaggat aagtcattac aatggaaata
     4381 acatcattgg gtttcacctt gtaattggct ttcaccggct tgtcgttgac cagaatattt
     4441 cctgcatcgg cagcttcctg aatccgatta cgtgaaacat tggccatgca attgaccaaa
     4501 tatttgtcaa tccgaatggg gctttgccct ttatccgcta taaatcgaaa atgttcaaaa
     4561 agttccggct cttcagccga atcggaaaaa tcttcccatt ttgttacttc caacaatttc
     4621 tttactcgat tttattggtt ttttaaaaaa atacttcgtc gttttccggc aaaattatta
     4681 tttggtcttc ttctttgtcc tccaataatt tagtgggaga agaggttaac caaatatcta
     4741 ttcgactgcc ggcaggcata gaagtccccg ctttaggaag ctgccgatat ataaaatagt
     4801 ctgcatcatt tcctttgggg ggaatatcaa agtttaccat ccctataaca aaagaattgg
     4861 aaagagcggc ttgtttggct tcctgcagtc ccattccttt tagcgaagga acagaaaaca
     4921 taccctcgcc ataaccactt ccaacaacca aaactactga actgctttca gggatttttg
     4981 tcccgggtaa aatactgact ccgttatatt ttatgtctat gaccaaatct ttgaactctg
     5041 atggcttata ttcaatttta cttatgtcaa gtcctacaga ttgcagcatg gcaatagctt
     5101 gacgagaaga aacatctata acttcaggaa ctggaacttt ttgaacttga gttgaattga
     5161 tgatcagata taccggtcta tttttcttaa gtgttgattt aggaggtgga acttgttcaa
     5221 taataattcc ggctttttta tctctaacat atacggaatc aataattttt ggatacaatc
     5281 ctttttctct taaaatattt tctgcttctt cgacgtacaa acctttcata tcagggacaa
     5341 tttcagcttc accatgatgt gtataaatat caattatgaa caatgttatc catgtcaaag
     5401 ctaaaaaaat tgcaatggcc agtaaaaggt ttttaactac aaatcctgca aaagattctt
     5461 tccaaaattt tttgaaatcc ataagttatg ttagggtctt ttgaacaaag ttgaagaagt
     5521 aaacttctgt gaaaaaattt attccaaaag tacagaaata attttattat cggtgtataa
     5581 caaacaaaaa aagtaaaaac ctctttcgaa gttcttactt tgaataacgt tagatgtaat
     5641 ttttttagtg tatctgatta caattttgga cgtccttcat cggaaactgt cagttttttt
     5701 cttccttttg ctctgcgtga agccaaaacc ctgcgtccat tggcagtagt cattctttca
     5761 cgaaatccat gtttgtttct tctttttctt tgcgaaggtt gaaatgtcct tttcatcgtg
     5821 tttggtattt gatgttgttt ttactccaga tttttagacc acaaaagtaa tgtttttttt
     5881 ttgaaatgcc aaacatagtt cagaaaatag atgctataaa aacagaaaac tcttttcgat
     5941 aaacttttta aagaaaattg agtttacagc tattgcatat tgcagtttaa aatcgttgtt
     6001 gtatttttaa cgaactaata actacttgcc ggctttgaga aacgaatatc atttaaaatt
     6061 tacttaatca ttaaaaaaag aattattttt ttaaactaaa aatggaatag tctcgttatg
     6121 tactaaacat ttttcgttag aaattgtttt tattaagata tgaattgttt atgttaattt
     6181 aatattgtta aatcttataa aaaactattt taattacttc attttaagta aatttgcaaa
     6241 agtatagtgt atgaaaaaga gaacgatctg gatattgatc attttaatgg tagttacaat
     6301 tgtgggtttg gtactcgtac aaatccgcta tgtacgaata aatgccgaaa tgatcgaaaa
     6361 ccagttcaaa gaaaacgttc agcgaagttt atttcaagct gtgcgactgg ttgaagaaaa
     6421 cgaagcattg cagtacttgt cggaaacatt ggaagatgtg gattattcag ccaaaagcaa
     6481 gggattaata aaaagtggaa atgcacaaaa agatgtgctg ggaatgacaa ccgacagcat
     6541 ggcgatggat tcaggtaaag actttacatt aaaccaaaaa ttgccgtcga tacaattatc
     6601 gacgaaacat ggtaaagcaa caattgaaga aacatcgcga tatttgcaaa aaaaattcca
     6661 acaggatttt tcacgttcaa aaaccatttt ggatcaggct gttttcagat ggctcagaga
     6721 gtcggatcaa aaggaaattt ctcaacgggt aaactttgac gaactgaacg acatactgca
     6781 aaccctctta gaaaacaacg gcgtaacgct gccgtttaat tattcagtag tggacaaaca
     6841 gggaagattg gtttacaagc ataccaatat ctcagtagat gagcaaaaaa tatcgcaaac
     6901 cggaaatgta tttacccaaa aacttttccc gtttgaggaa accaaccaac cttactacct
     6961 gcaagtagat tttcctacca ggcaaaatta cattttgaat tcgatgaatt tattactgcc
     7021 atcggttgtt ttggtaatac ttattctgac aatttttatc attgcgatta tcattatttt
     7081 caggcaaaaa caattaaaca acatcaagaa tgattttgtg aacaacatga cacatgaact
     7141 gaaaactccc atcgctacta tttcgttggc ttcgcagatg ctgcaggatc caaacattgg
     7201 aaaaacgccc gaaatgctcc aacatgtatc aaaagtaata catgatgaaa cacaacgatt
     7261 aagttttcag gtagaaaaag tactgcaaat ggccattttt gaaaaagaaa aatcgccttt
     7321 aaatttgacc gaaatacata ttaattcttt gattcaggat gttatacaca gtttttcgtt
     7381 gagagtaacc aacagtggtg gaaaaattat tccttcgttg aaagccgaaa aagatttggc
     7441 actgattgat gaagttcatt tcacaaacgt gattttcaac ttaatggaca atgcgctgaa
     7501 atatagcaac aagcctctgc tcctaaatat cgaaacatgg aacgaaaaag acagaatatt
     7561 cattagcatt gaagacaacg gaataggtat tcgaaaagaa gacttaaagc ggatttttga
     7621 aaagttttat cgggtttcca ccggaaacct gcacaatgtg aaaggatttg gacttggatt
     7681 ggcttatgta aaaaaaatca taaccgatca taaagggaca attaaagtgg aaagcgaacc
     7741 caatattgga actaaattta ctatttcatt accaatttta aaaaatcaag aaaaatgaca
     7801 gacgaaaaaa ttaaaatttt attgtgcgaa gatgacgaaa atcttggcat gattttaaga
     7861 gagtatctgc aatcgaaagg atacgatgta gatttgttgc ctgacggcga agccggctat
     7921 aaagccttta cgcataaaaa atacgatttg tgcattttag acgtcatgat gccaaagaaa
     7981 gacggattta cactggcagc cgacattcgt gcgctgaata gcgaagttcc cattgtcttt
     8041 ctgacagcca aatcgatgaa agaagatatt ttgcagggtt tcaaactggg ggcagacgac
     8101 tatttgtcga aacctttcag tatggaagag ttgctgtatc gaatcgaatc gatcatgcgc
     8161 cgtatcagag gcgaaaaggc cagagaagtt accgtttatc agattggcaa ttacacattc
     8221 gactctcaaa agcaactgat taccattaat ggtgagacca aaaagctgac cacgaaggaa
     8281 tcggaacttt tgagcctgct ggctgccaat atgaacaacg tgctggaacg taattacgca
     8341 ttaaaaacca tttgggtgga tgacaattac ttcaatgcca gaagtatgga tgtttatatc
     8401 accaaacttc gcaaattgtt gaaagacgat cccaacgtgg ccatcattaa cattcatggc
     8461 aaaggttaca agttgattgc tcctgccgta aaagaaaaga attaaatcat taactttcag
     8521 atttttaaga ggctgttccg ttttttcaga gcagcctttt ttatttttac ccgaaaaaat
     8581 ttttaaggaa aagtatcaac attcaaaaat ttggcttaaa ttagcaccct tttctaaaaa
     8641 aaaaatcaaa tgaaacatgc tgaataaagg cttgttgatt tttgatgaat agaaataacg
     8701 aaatagcttc aatattatga attaaaaaat cgacttatga aattgcgtaa ttttatcctg
     8761 accgttctat tattttcatt tttgctcact tatgccgaaa ttcctgccgg ttattacaac
     8821 agtgctgacg gtaaaaatgc ttctgcattg cgaacggctc ttaaaacaat cattaccaac
     8881 ggacattctg taacgagtta tgccggattg tggacagcat acaaaactac cgatgtgaat
     8941 gcaaacggta aaatttggga catgtattcc aattgctcat ttacttatgg taccgatcaa
     9001 tgtggcagtt acagcaacga atgcgactgc tacaacagag aacataccac accacaaagt
     9061 tggtttaacg aagcccctcc gatggtttcc gatttgttta atgtttatcc gactgatggc
     9121 aaagtgaacg gcatgcgaag caattatccc tatggagaag ttggttcggc tacatacacg
     9181 tcgggcaacg gaagtaaatt ggggagcagt tcattttccg gttacagcgg gactgttttt
     9241 gaacctatag acgaatacaa aggcgatttt gcacgaacct atttttatat ggcaacgcgc
     9301 tatgccgatg aatgcggaac atggacaggt gaagctactg ccgtttatag ttcttctaat
     9361 ggattgacgg aatatggaaa aaatcttttc ctaaaatgga gtcgtgaaga tccggtgagt
     9421 cagaaagaaa tagaccgaaa caatgctgtg tatgcaattc aaaacaacag aaatccgttt
     9481 atcgattatc ccggtttaga ggaatatatt tggggaacaa aaacttcgga agttttttat
     9541 gtcaatgctt ctgtttccaa gccttctgtg tcttcaccaa ccgccacgaa tgtaacctat
     9601 aacagtgctg ttttgggcgg aacagttaca agtactggag gcggaaccat tacggaaaca
     9661 ggagtatatt acagcactac cgatggtttt gccgatggaa caggtaccaa agtatctact
     9721 tcttctcctt caacgagtac gtttacagtt tccataaacg gattaaactc ttctacaaca
     9781 tattatttca aagcttttgc aaccaacagt gcaggaacgg gttattcttc tcaatcaagt
     9841 tttaaaactt cgaccttaag ttcgggaaac gacccagaaa ttattctggg aaatccggtt
     9901 gcaactggat cgacattaaa tttcggaaaa gttacggcaa attccaccaa atcattgttg
     9961 ataaagacca aaaatttgac cggagatctt tctgtcagcg ttacgggaga aatgttcaac
    10021 gtttctacca gcactatttc caagtcaaat gcagaagaag gttatctgct caatgtcaat
    10081 taccagccga cagcaaccgg ctctcatacg ggtactttaa ctatttcagg tggaggatta
    10141 actcccgatt atgtcgttca gttggcaggt tcaaaataat aggatacggt ttcccgcaaa
    10201 taaagcaaat agcgttgtcg gattcaatgg caacgcttta agtcatgaca aaaatttaaa
    10261 aatagaatcc cctcttaaaa tctgattttt tcaaaaaaga gaaaaaataa gatttttttg
    10321 aagagaagta ttaaattttt tgttgtttcg atattttaca ataatttgca atataacagt
    10381 tatttttgtt acaatggtaa cctaattgaa aaatggtcgc ttcaacattg tttctatggt
    10441 taagttcttc ttttggaatg tttcgtagtt tttgtatcaa aacagaagtt cg
//
LOCUS       DAOSCH010000073        10256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4295_length_10256_cov_5.936183, whole genome shotgun sequence.
ACCESSION   DAOSCH010000073 DAOSCH010000000
VERSION     DAOSCH010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10256
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4295_length_10256_cov_5.936183"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1265)
                     /locus_tag="PLZ54_06390"
     CDS             complement(<1..1265)
                     /locus_tag="PLZ54_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOJ66591.1"
                     /translation="MNISEFQQLYAHHPQMLRVKEWANSLEQNLKISGLSGSSSALVF
                     SSLFKAVPQHHIIIMNDADEAGYLYNDLKLLLSENEIYFFPSSFKRAIKLSQLDAAND
                     ILRTEVLNRLANNSQPCIVVTYPDAIMQKIVSLQSLRQKTLKLHIGENIGIDFINELL
                     FDFGFQRVDFVYEPGQFSIRGGIVDIFSFANELPYRCDFFGDEIESIRVFDIETQLSK
                     EEKQEIEIVPDLQRENKLPHVSFFEFIPKNTLLSFTHPNFIHDRINQLYDEFLVKANS
                     EGHETPHLHKTMITGDQWLELIEPFRKIDWGTDRTFFKTSNQISFNTSPQPVFHKNFD
                     LISDHLKKKIEEGYRIYIMSDSVKQTDRIAAIFADRGDNIHFQPIKSTLHEGFVDHDL
                     SLLCYTDHQIFDRFHKYQLSTDKTRQGKVV"
     gene            complement(1309..1779)
                     /locus_tag="PLZ54_06395"
     CDS             complement(1309..1779)
                     /locus_tag="PLZ54_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOJ66592.1"
                     /translation="MEFQEKMMRKAIALSLENIKKGGGPFGAVIVKDGKIIATGVNRV
                     TSNNDPTAHAEITAIRKAAGKLKNFDLSGCEIYTSCEPCPMCLGAIYWAHLDKMYFAN
                     TKADAQNAGFDDSFIYEEINLKPEDRKLKSQQLLREEALAAFESWKNTTNKTEY"
     gene            2197..3252
                     /locus_tag="PLZ54_06400"
     CDS             2197..3252
                     /locus_tag="PLZ54_06400"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOJ66593.1"
                     /translation="MKKNFLPLLLLFLNLTVVFSQPKQGYYNQAVGKSGAELKTTLYS
                     IISSGYVSKSYDFLYTIYETSDVTPEGLIWDMYSDCTWVPGQKKCGNYSKVCDCYNRE
                     HSIPQSWFNSQSPMVSDAFHVYPTDGKVNGQRSNFPYGECSGGITLSGGRGRLGNSTF
                     TGYSGTVFEPDDEYKGDFARTYFYFATRYQNIMTTIDGDSFNKTTYPALSDWAKALFL
                     KWHRQDPVSKKETDRNNAVEVYQKNRNPFIDHPELAEYIWGTKVGEPWSLSSGIENLK
                     IDFSISPVPAQDFLNIKTDENELMCTIFNIQGQELMKTLIDGNSSISVSQLNNGMYLL
                     QLEKDGKKTIQKFMVSR"
     gene            3338..5506
                     /locus_tag="PLZ54_06405"
     CDS             3338..5506
                     /locus_tag="PLZ54_06405"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOJ66594.1"
                     /translation="MKKFFSTLFLILIVWLNCQIAFSEVPAGYYYLAHNKKKAELKSA
                     LSQIASPLRVLNYGSGKGYTWEGFYYADQNPDGSVKDMYSSEIRYFKDFSSVAGMHIE
                     HSLPKSWWGGHENNAYKDLFHLYPADGVTNSTKNNLPLGEVTGTPNFDNGVSKIGKNG
                     FGGIYTDNCFEPADEYKGDFARSYLYVSTVYENFATLWNSPMMNNNTYPVWKKWAIDL
                     LLKWNREDPVSQKERDRIEVVYNIQGNRNPFIDYPDLAEYIWGADTTKFFPFPEETEP
                     FLIYPKRGDQMDFGVILQTDSIQQHLTVSGLNLQNNLNIRLKGKSKSFKISKASLTKE
                     EVINSTEVIITFRPVVSGWNRDTLLLTSDGWKDSLRIPLQGLASTDFLALEPQNITPT
                     GGTLRWIFDPDADNYKLNLYQGDQQAGDLIISAYVEGSSWNKALELFNGTGKNIDLSN
                     YSLQKQSNGDGNFGATLKLNGMLENGKTYVLVHKSCSNNDLKAKANLLTDSLLSFNGN
                     DAVALLRNGVFIDVVGIANAGADVYWGQDLTLKRKATVTHPSSQFRDIEWNFYGIDDF
                     QMLGNHQMNLQPSSNYLLNDFETGKTDHFTISQLLPENTYTYSVTAIYPDEEKKALNT
                     MQIHTSKLEVPVIMNATDVRANSFVAGWEDCLYTSDYLLDVFQIQSGKDTTETESFDN
                     IGSNGKPLPEGWSGTASGNYTTAASSGKNPNALAFKNDGE"
     gene            5552..6172
                     /locus_tag="PLZ54_06410"
     CDS             5552..6172
                     /locus_tag="PLZ54_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOJ66595.1"
                     /translation="MYRFPSGATGSSLIVEAVKNGNYVRIDSIGSVNTSKNYPMYTFQ
                     ESDDIHSFRFIYKKVSGNLALDDVEATYKQQDTVFVLKNFQVSGSQFKVEGLNPNSVY
                     FYRVRSTIHPYTSDFSETIQVKTGFPSALENIPASQYKIYISSGDIKLSNLQGNERVQ
                     LFNIAGVCLFDQHSSGSEMSIRIPHRGIFIVRISNKDFTFARKLIK"
     gene            6187..7269
                     /locus_tag="PLZ54_06415"
     CDS             6187..7269
                     /locus_tag="PLZ54_06415"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOJ66596.1"
                     /translation="MKFSFCFIKKISVVVLTFFSLTLSAEIPEGYYRTAEGKTGAELK
                     TALHNIIRQHVILNYDTSTTVWWYTYFKTTDWHPDGYFWDMYSNNKRSTYNSSVMNRE
                     HCMPRSWWGTSSNYSTFDANGDLVNLSPSDAEANSAKSNYPLGIVGTASFDNGVVKVG
                     NNTYPGYTGLVFEPANQYKGDFARTYMYMVTCYEDYHSYWRGTGTSSMLDRNTYPVFK
                     QWAIKMLLEWSRNDPVSDKEINRNDGVYRIQQNRNPFIDHPELAEYIWGTSKGEVWNG
                     TAGNNDLYVNLSVYPNPASTSVLVNLNKPESSYYRIYDLSGISVKIGNFSSDGKLDVS
                     ELQIGSYLLVVYYGNQRKTIKLMIAH"
     gene            7559..8851
                     /locus_tag="PLZ54_06420"
     CDS             7559..8851
                     /locus_tag="PLZ54_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ66597.1"
                     /translation="MKTKKNFHSFPFKLWSLVLLSLPVCLPSQNNFQKNFQKNDSDYV
                     EFSGKIVDVKNGDVLPFATLSVEGSNITTVSNSEGEFSLKIPKEFQDKTLTVSYIGYK
                     NRILKISELKQENSRITLEPMIVSLPEINVISTDAENLVLSVLENRYKNYPEEELLMT
                     AFYRETIKKNRSYVSLSEAIVDILKQPYSSFRQDQAELYKSRKQSDYKKMDTLVFKLM
                     GGPYNSLYMDVMKNPDYLFTESILDNYEFSFDRTTYIDHQPIYIVNFKQRPFVNIPLY
                     YGKLYIDAESLALRSAIFHLDLTDKEKSASMLIRKKPLNAKVFLTNASYRMDYIEKNG
                     KWYYNYSRIELDFKIDWKRKLFNSNYYTVVEMAVTNWEKATEEKKLKNKLRPTVIIAD
                     EASGFSDPEFWGSYNVIEPEKPIESAIKKIQKQLEKRK"
     gene            9294..10178
                     /locus_tag="PLZ54_06425"
     CDS             9294..10178
                     /locus_tag="PLZ54_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HOJ66598.1"
                     /translation="MKKKIAFIINPMAGVRTKSFIPQHIHDELNRDLFDPTVAFTDYR
                     GHGSLLARDFAAKGFDSVVAVGGDGTINEVARGLVQTSVSLGILPIGSGNGLARHLEI
                     PMNMRKAIQMLNFSEEISIDYGLVNQRPFFCTCGTGFDAYIAAEFAKGHRRGIVAYLE
                     KIVKGYFAYHSENYRIIGKNIDFTTKTFLMTIANASQWGNNVYIAPKASVQDGRLDVS
                     IMSNFPIIALPTLALQLFTKTISNDLFINTFRMSEITVFRPAPGPFHYDGEPYEEGKE
                     INIRVVPAGLKVLVKKRF"
BASE COUNT         3115 a         1847 c         2041 g         3253 t
ORIGIN      
        1 accacttttc cctgacgggt tttatcggta cttaactggt atttgtgaaa acggtcgaaa
       61 atctgatgat cggtgtaaca caaaagagaa aggtcgtggt cgacaaaacc ttcgtgaaga
      121 gtggatttaa tgggttgaaa atggatattg tcgcctcgat cggcaaaaat ggcagcaatg
      181 cgatccgttt gtttcacgct gtcgctcatg atgtaaatgc ggtaaccttc ttcgattttc
      241 tttttcaaat gatcgcttat caaatcgaaa tttttatgaa aaaccggctg tggtgaggta
      301 ttgaaagaaa tttggttcga agttttgaaa aaagttctat cggttcccca gtctattttt
      361 ctaaacggtt caatgagttc cagccactgg tctccggtaa tcattgtttt gtgaagatgc
      421 ggcgtttcat gtccttcgga atttgctttt accaaaaatt cgtcataaag ttgatttatg
      481 cggtcatgaa taaaatttgg gtgcgtgaaa ctcagcaggg tatttttagg aataaactca
      541 aaaaaggaaa catgaggcaa tttattttcc cgctgaagat cggggacaat ttcaatttct
      601 tgcttttcct ctttggacag ttgggtttcg atatcgaaaa cccgaatgga ttctatttcg
      661 tcgccaaaaa agtcgcatcg atagggcaat tcattggcaa aggaaaaaat atccacaatt
      721 cctcctctaa tcgaaaattg cccgggttca tatacgaagt caacccgttg aaaaccaaag
      781 tcgaacaaca attcgttgat aaagtcgata ccgatatttt cgccaatgtg aagtttcaag
      841 gttttttgac gcaaactttg aagcgaaaca attttttgca tgatcgcgtc gggataggta
      901 acaacaatgc acggctgaga attattggca aggcggttca gtacttcggt tcttaaaata
      961 tcgttggcag cgtcgagttg cgacagtttg atggctcttt tgaaagagga agggaaaaaa
     1021 taaatctcgt tttcagacag caacagtttc aggtcgttgt acagatatcc tgcttcgtcg
     1081 gcatcattca ttattataat atggtgctga ggaacggctt tgaacagtga cgaaaaaacg
     1141 agtgcagatg acgaaccgct caaaccggaa atttttaaat tctgttcgag tgaatttgcc
     1201 cattctttta cccgcaacat ctgggggtga tgagcataaa gttgttgaaa ttcagagata
     1261 ttcaatatgc aaattaacta ttttaaatgc agttgccaaa agcaactttc agtattctgt
     1321 tttatttgtt gtgtttttcc atgattcaaa tgctgcaagg gcttcctctc tgagcagttg
     1381 ttgcgatttt aattttctgt cttcaggctt cagatttatt tcttcgtaaa tgaaggaatc
     1441 atcaaatccg gcattttgcg cgtcggcttt ggtgttggca aaatacattt tgtcgagatg
     1501 agcccaataa atagcaccca gacacatcgg gcaaggttcg cacgaagtat aaatttcgca
     1561 acccgacagg tcaaaatttt tcagtttgcc ggctgctttt ctgatggccg ttatttcggc
     1621 atgtgcagtg ggatcgttgt tggaggtaac acggttgaca ccggttgcaa tgattttccc
     1681 atctttcacg attaccgcac caaacgggcc tcctcctttt tttatattct ccaaagacaa
     1741 tgcaattgct ttacgcatca ttttttcttg aaactccatg tcgttgacgt attctacagt
     1801 ttctttttgg ttcttgaaac atcaatacaa agataatatt ttttcagtaa acaggttata
     1861 cctgctagtt acatactatt ttttgaataa accttttaaa aaaaaattcg gattaaatat
     1921 tcacgttatt ttggtcaatt ttagctgaat gtacctgaat tataggtttt aatttttcct
     1981 tagaatgaat ttttcaaaca ttttattaat ttcacatttg ccgattaact ctacagcctt
     2041 ctattgtctt tttttaactt gccatttaaa aataatgttt tatctttgaa gccctaaatt
     2101 tttaggaaat tttataaata ttcatttttt aaacattttt atttgtttat tgcataaaaa
     2161 ctcatttact catttgttta attgaattaa ttcatcatga agaagaactt tctcccattg
     2221 ttgttgcttt ttttgaatct gacagttgtt ttttctcaac ccaagcaggg ttattacaat
     2281 caggccgtag gaaaaagcgg tgccgaactc aaaaccactc tttattccat tatttcatca
     2341 ggttatgtaa gcaaaagtta tgattttctg tacaccattt acgaaaccag cgatgttact
     2401 cccgaaggtc ttatttggga catgtattcc gactgcactt gggtgcccgg acaaaaaaag
     2461 tgcgggaatt atagcaaagt ttgcgattgt tacaaccgcg aacattccat accgcaaagc
     2521 tggtttaatt ctcaatcgcc tatggtaagc gatgcttttc atgtttatcc caccgatggg
     2581 aaagtgaatg ggcagcgaag caatttccct tatggcgaat gtagcggtgg aattaccctt
     2641 tccggtggaa gaggaagatt gggaaattcc acttttacag gatattcggg aacagttttt
     2701 gaacccgatg atgaatataa aggcgatttt gcacgtactt atttttattt cgccacacgt
     2761 tatcagaaca ttatgactac cattgatgga gattcattta ataaaacaac ttatccggcg
     2821 ctctcggatt gggcgaaagc attatttttg aaatggcacc ggcaggatcc ggtttcaaaa
     2881 aaggaaaccg acagaaacaa tgccgtagaa gtttatcaaa aaaacaggaa tccttttatt
     2941 gatcatccgg agttggcaga atatatttgg ggcacgaaag ttggtgaacc ttggtcgctt
     3001 tcttcaggaa ttgaaaatct gaaaatagat ttttccataa gtcctgtgcc ggctcaagat
     3061 tttctgaata tcaagactga cgaaaacgaa ttaatgtgta ctattttcaa cattcagggg
     3121 caggaattaa tgaaaacctt gattgatgga aattcttcta tatctgtatc tcaactaaat
     3181 aacggaatgt atttgctgca actagagaaa gatgggaaaa aaaccattca aaaatttatg
     3241 gtcagcaggt aatggcagca acttttatat tttacaaaat agtgatgata aataaggatg
     3301 atcttaaaaa cgtctcgcat ttaatttgaa cgaaaaaatg aaaaaatttt tcagcacgct
     3361 ttttttaatt ttaattgttt ggctaaactg tcagattgca ttttcggaag tgccggctgg
     3421 ttattattat ttagcccaca acaaaaagaa ggcagaatta aaatcagctc tttctcaaat
     3481 tgcatctcca ttgcgtgtcc tcaattatgg cagtgggaaa ggatatacat gggaagggtt
     3541 ttattatgcc gatcaaaacc cggatggttc ggtaaaagat atgtattctt cggaaatacg
     3601 gtacttcaag gatttttctt ccgtcgcagg catgcatatt gagcactcat tgccaaaaag
     3661 ttggtggggc ggacacgaaa ataatgcata caaggatttg tttcatttgt atccggccga
     3721 tggagttacc aattctacaa aaaacaatct tccgcttggg gaagttaccg gcacgccaaa
     3781 tttcgacaat ggggtaagca aaattgggaa aaatggtttt ggaggaattt ataccgacaa
     3841 ttgctttgag cctgccgatg aatataaagg cgattttgcg cgttcttatc tgtacgtgtc
     3901 aactgtttac gaaaattttg ctaccttgtg gaattcgccc atgatgaata acaacactta
     3961 tccggtgtgg aaaaaatggg ctatcgattt attgctgaaa tggaatagag aagaccctgt
     4021 cagtcaaaaa gaacgcgatc gtattgaagt tgtttacaac attcagggaa atcgcaatcc
     4081 ttttattgat tatcccgatt tggcagaata tatttggggt gccgatacaa caaagttttt
     4141 tccttttcct gaagaaacag agccgtttct gatttatccc aaacgcggtg atcaaatgga
     4201 tttcggcgta atattgcaaa ccgattccat tcaacagcat ttaactgttt caggattgaa
     4261 tttacaaaac aatttgaata tcagacttaa gggcaaaagt aaatccttta aaatttccaa
     4321 agcttcgttg acgaaagaag aagttatcaa tagcactgaa gtaattatca cttttcgtcc
     4381 tgtggtttca ggctggaaca gagatacgct tttgctgact tccgacggtt ggaaagattc
     4441 tttgcgtatc ccactgcaag gtttggcttc gactgatttt cttgccttag aaccgcaaaa
     4501 tatcactcct acgggaggaa cattacgatg gattttcgac cccgatgccg acaactacaa
     4561 actgaatctg tatcaaggcg atcagcaagc cggcgatttg attatttccg cttatgtgga
     4621 aggcagtagc tggaacaaag ctttggaatt gttcaatgga accgggaaaa atatcgattt
     4681 atccaattac agtctccaaa aacaatccaa tggagatgga aattttggag ctacactgaa
     4741 attgaatgga atgctggaaa acggaaaaac ttatgtgttg gttcacaaaa gctgttcaaa
     4801 caacgatttg aaagcaaaag ccaatttgtt aacggattca ttactcagtt ttaatggaaa
     4861 cgatgccgtt gctttgttga gaaatggtgt ttttattgat gttgtaggca tcgccaatgc
     4921 aggtgctgat gtatattggg gtcaggattt aactttgaaa cgaaaagcta cggttacgca
     4981 tccaagttct caattcagag atatagaatg gaatttttac ggaatagatg attttcaaat
     5041 gcttggaaat catcaaatga acttacaacc gtcctccaac tatctgttaa atgattttga
     5101 gaccgggaaa acggatcatt tcaccatatc gcaattgtta cccgaaaata cttatactta
     5161 ttccgtaacg gcaatatatc ccgatgaaga aaaaaaagct ttgaacacca tgcagattca
     5221 tacttccaaa cttgaagtgc ctgtaattat gaatgcaacg gatgttcgag ccaacagttt
     5281 tgttgccggt tgggaagatt gcctgtacac gtccgattat ctgctggatg tttttcaaat
     5341 acagtccggc aaagatacaa ctgaaaccga atcgtttgac aatattggaa gcaacgggaa
     5401 acctttgcct gaagggtgga gtggaacagc aagcggaaat tatactaccg ctgccagctc
     5461 cggtaaaaat cccaacgcgt tggcatttaa aaatgatgga gaatagttac aaaccaaaac
     5521 ttatccgagc tgggtttcgc aactaaaatt tatgtatcgt ttcccttcag gagcaaccgg
     5581 ttcatcgctt attgtcgaag cagtaaagaa tggaaattat gttcgtatcg acagcattgg
     5641 atcggtaaat acttcaaaaa attatccgat gtacactttt caggaatcgg atgatattca
     5701 ttcctttagg tttatttaca aaaaagtatc aggcaacctg gcacttgacg atgtggaggc
     5761 aacttacaag caacaggaca cagtttttgt gttgaaaaat tttcaggttt cgggaagtca
     5821 atttaaagtg gaagggctga atccgaattc agtttatttt tatcgggttc gttcaaccat
     5881 acatccatat acatcggatt tttctgaaac tattcaggtg aagaccggat ttccttcagc
     5941 tttggaaaat atacctgcaa gccaatataa aatttacata tccagtggcg atattaaatt
     6001 gtcgaacttg caaggcaatg agcgtgttca actttttaat attgccggcg tatgtttgtt
     6061 tgatcaacat tcatcaggat cggagatgag tataagaatt ccgcacagag ggatttttat
     6121 tgttcgtatc tcaaataaag attttacttt tgcacgaaaa ttaataaaat aaatttttct
     6181 tagtacatga aattttcttt ttgctttatt aaaaaaattt cagtagttgt tctgacgttt
     6241 ttcagcctta ctctttcggc cgaaatacct gaaggttatt accggactgc tgaaggaaaa
     6301 acaggggctg aattaaaaac tgccttgcac aatatcattc gacaacacgt tattctgaat
     6361 tacgacacca gtactaccgt ttggtggtac acttatttta agacaaccga ctggcatccg
     6421 gacggttatt tctgggacat gtattccaac aacaaacgtt ccacttataa tagcagcgtt
     6481 atgaatcgtg aacactgcat gccgcgaagc tggtggggaa caagttcgaa ttattcaaca
     6541 ttcgatgcca atggcgattt ggtgaatctt tctccttcgg atgccgaagc caactcggcc
     6601 aaatctaatt atccattggg aattgttggt actgccagtt tcgataatgg ggtggtaaaa
     6661 gttggaaaca atacttatcc cggttacacc gggttggttt ttgagccggc taatcaatac
     6721 aaaggcgatt ttgcacgaac ttacatgtat atggttacct gttacgaaga ttatcattcg
     6781 tactggagag gaaccggaac ttcgagcatg ttggacagaa atacttatcc cgtattcaaa
     6841 caatgggcca taaaaatgtt gctggagtgg agcagaaatg acccggtcag cgacaaggaa
     6901 atcaaccgga atgatggtgt ttatcgaatt caacagaatc ggaatccatt tattgaccat
     6961 cctgaactgg cagaatacat ttggggcaca tcaaaaggcg aggtatggaa cggaacagcc
     7021 ggcaacaatg acctgtatgt gaacctgtcg gtttatccca atccggcatc gacttctgtg
     7081 ttggttaacc tcaacaaacc cgaaagttca tattatcgta tttacgacct cagcggaata
     7141 agtgtgaaaa tcggtaattt ctcttcagat gggaaattgg atgtttccga acttcaaatt
     7201 ggttcgtacc ttttggtagt atattatgga aatcagcgaa aaacaataaa gttaatgata
     7261 gctcactgat aatttttggt ttttttcgca atttttcatc agaatataat ttattttttt
     7321 aaggtctgat ggaacccaca tgtctgaata ttatatttat tcgtgtttgt gtatgcaata
     7381 catgtgggtt tcttatttgt ttttgcggca tttgatgtct caaacttttg tccagccaaa
     7441 ttgttatttt taagtgaaat atagcacaat tccatgttga atgtgttatc tttgtttgta
     7501 agaatttttc acgtagattc aacaatttat ccggttctac tctttataac ttgccgccat
     7561 gaaaacaaaa aaaaatttcc attctttccc gtttaaatta tggagcttag ttttgttatc
     7621 gctccctgtt tgcttgccat cgcaaaataa ttttcaaaaa aattttcaga agaacgattc
     7681 tgattatgtg gaattttcag gtaaaattgt agatgttaaa aacggagatg tattgccatt
     7741 tgccactctt tcggtagaag gaagcaatat cactacggtt tccaattccg aaggagagtt
     7801 ttccttgaaa attccaaaag aatttcaaga taaaacacta acggtcagtt atataggata
     7861 caaaaataga attcttaaaa tttccgagct aaaacaggaa aattctcgca taactttgga
     7921 acccatgatt gtttctttgc ccgagataaa tgtgatttca acagatgcag aaaatttggt
     7981 actgtcagta ttggaaaatc gctacaaaaa ttatccggaa gaagaactgt taatgacagc
     8041 tttttacaga gaaaccatca aaaaaaatcg ctcttatgta tcgctttctg aagccattgt
     8101 ggatattctt aaacagcctt attcttcatt ccggcaggat caggcagaat tgtataaaag
     8161 caggaaacaa tccgactata aaaaaatgga tacattggtt tttaagttga tgggtggtcc
     8221 ttacaattcg ctttacatgg atgtgatgaa aaatcccgac tatcttttta ctgaaagtat
     8281 cttggataat tatgaatttt cttttgatcg gactacttac atcgatcatc agccaattta
     8341 tattgtcaac tttaagcaac ggccattcgt caacattccg ttatattatg gaaaacttta
     8401 cattgatgct gaaagtcttg ccttgagaag tgccattttt catcttgatc ttaccgacaa
     8461 agaaaaatca gcttccatgc tgatacgcaa aaaacccctt aatgcaaaag tgtttttaac
     8521 aaatgcctcg tatcgaatgg attacattga aaaaaatggg aaatggtact ataactacag
     8581 tcggatagaa ttggatttta aaattgactg gaagagaaaa ttattcaatt ccaattacta
     8641 tacggtagtg gaaatggccg ttaccaattg ggaaaaagct actgaagaaa agaaattgaa
     8701 aaacaagttg cgcccgacgg taattattgc agatgaagct tccggctttt ctgatccgga
     8761 attttggggc agttacaatg tgattgaacc cgaaaaaccc atagaatcag caataaaaaa
     8821 gattcagaaa caactcgaaa aaagaaagta attttttcgt cattttctct gccgagaaac
     8881 tttgcgacta ccgattgctc aaatttttgt ctttcatcag aatataattt attttttaaa
     8941 ggtctgatgg aacccacatg tctaaatatt atattcattc gtgtttgtgt atgcaataca
     9001 tgtgggtttc ataaacagta ttaattttag gaagacttcg gtctcctttt ttggtgaatt
     9061 atgttgtcgg ttcattttaa tgctctttca agcttttact tccttccagt cgtttgccta
     9121 tcagctgtgg tgctttgggt ttattaaaaa acaatgtaaa actttttgct taattcgcac
     9181 ttctaagttg aacttaaccg gattctataa tgttgaataa ttattgatga attttgcagt
     9241 ttaatagttt tttgtaactt cgcagccgtt ttttgaacgc acgtttttct gccatgaaaa
     9301 agaaaattgc tttcattatc aatcctatgg ccggtgtaag gacaaagtcc ttcatcccac
     9361 aacatatcca cgacgaatta aacagagatt tgtttgatcc aactgtggcg tttaccgact
     9421 accgtggtca tggaagctta ttggccagag attttgctgc taagggtttc gatagcgtgg
     9481 tggcagttgg tggtgatgga acgattaacg aagttgcccg cggactggtt caaacttcgg
     9541 tttcgttagg aattttaccc attggttcgg gaaatggttt ggcgcgacat ttggaaatcc
     9601 caatgaatat gcgaaaagcc attcaaatgc tcaatttttc ggaagaaatt tccatcgatt
     9661 acggattggt aaatcaacga cctttcttct gtacctgtgg aacaggtttc gacgcttaca
     9721 ttgctgccga atttgccaaa ggccatcgca gagggattgt ggcttatctg gaaaaaattg
     9781 tgaaaggtta ttttgcctat cattctgaaa attaccgtat tattgggaaa aatatcgatt
     9841 ttaccaccaa aacttttctt atgaccatcg ctaatgcttc tcaatgggga aataatgtgt
     9901 acattgcccc aaaagccagc gttcaggatg gacggctcga tgtttccatt atgagtaatt
     9961 tccccattat tgctcttcct actcttgcac tgcaactttt taccaagact ataagcaacg
    10021 acttgtttat caatactttc cgaatgagtg aaattacggt ttttcgacct gcgcccggcc
    10081 cttttcatta tgatggcgag ccctatgaag aaggtaagga aataaatatc cgggttgtac
    10141 ctgccggttt gaaagtattg gtaaagaaaa gattctgaat accaaatttt ttcaggatgc
    10201 gtttttgctc aaaggaaatg attaattttg catccgaatt tcttctcgat ttttac
//
LOCUS       DAOSCH010000074        10214 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4313_length_10214_cov_5.303967, whole genome shotgun sequence.
ACCESSION   DAOSCH010000074 DAOSCH010000000
VERSION     DAOSCH010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10214
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4313_length_10214_cov_5.303967"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1169
                     /locus_tag="PLZ54_06430"
     CDS             279..1169
                     /locus_tag="PLZ54_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOJ66599.1"
                     /translation="MEHHHQHTHTHGESNIKMAFFLNFGFTVFEIFGGIFTNSIAIIS
                     DAIHDMGDSLSLGLAWYFQKKSTKESDKNYTYGYKRFSLLGAIINSIILITGSIFVLY
                     EAIPRLLHPETTKTQGMIVFAVIGIVVNGLAVLRLKKGSSINEKVVSLHMLEDVLGWA
                     AILVGSLIMYFFDLPVIDPVLSVLITIYILFHVFRNVKESLHILLQGSPTDVDMEKIS
                     QLLVSIENVDEIHDLHIWSIDGNYNILTVHVVLKQPVTIEKAVEIKNKIRSELLQQGI
                     EHATIEFETTEENCSLQQCC"
     gene            1315..1596
                     /locus_tag="PLZ54_06435"
     CDS             1315..1596
                     /locus_tag="PLZ54_06435"
                     /inference="COORDINATES: protein motif:HMM:NF015889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015661 - L-lysine efflux transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903401 - L-lysine transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysO family transporter"
                     /protein_id="HOJ66600.1"
                     /translation="MFTVLGCMLAGMVVGFIFRKKHFKIIQSVLFVLIWLLLFLLGAE
                     IGSNPAVIRQTGKLGFDALLIGTAGTLGSILGADLLWKWIKPDKSTHEK"
     gene            1586..2199
                     /locus_tag="PLZ54_06440"
                     /pseudo
     CDS             1586..2199
                     /locus_tag="PLZ54_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666827.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
     gene            2301..3932
                     /locus_tag="PLZ54_06445"
     CDS             2301..3932
                     /locus_tag="PLZ54_06445"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOJ66601.1"
                     /translation="MAKEFKYQELFQLGKDKTEYYLLSKDFVSTAEFEGKEILKVQPE
                     GLTFLARTAFRDCSFLLRPEHQKQVAAILSDPEASENDKYVALTMLRNAEISAKGILP
                     FCQDTGTATIYAKKGSNVFTDGTDEEALSKGVYETYTQENLRYSQNAPLTMYDEVNTG
                     TNLPAQIDIMVGHGNEYEFLFIAKGGGSANKSFLFQETKALLQPDTLEKFLTEKLKLL
                     GTSACPPYHIAFVIGGTSADACMKTVKLAAAKYYDELPTTGNELGRAFRDVELEEKIL
                     KAAQNLGIGAQFGGKYFAHDVRIIRLPRHGASCFVGLAVSCSADRNIKAKINKDGIWL
                     EKLEDNPGKYIPEEYRNTGEGNAVKIDLNRPMNEILSTLTKYPVSTRLSLSGTMVVAR
                     DIAHARIKEMLDAGKDLPQYMKDHPVYYAGPAKTPKGMPSGSFGPTTAGRMDTYVDLF
                     QEHGGSMVMIAKGNRSQQVVDACKKHGGFYLGSIGGPAAILAQNSIKKVECIDFPELG
                     MEAVWKIEVEDFPAFILIDDKGNDFFKLIKPTMTC"
     gene            4239..5687
                     /locus_tag="PLZ54_06450"
     CDS             4239..5687
                     /locus_tag="PLZ54_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOJ66602.1"
                     /translation="MQEIKFYKGENIPLELHKVRVVQKLHLVPIERRLEALQEGGYNT
                     FRLSTKDVFLDMLTDSGTNAMSDNQMAGMMQADDAYAGSQSFYRLQKAVQDVLGKKYY
                     LPVHQGRAAENIISYAYVKKGSIIPMNYHFTTAMAHITQYGGKIAELLYDEAYVIDSE
                     HPFKGNMNLEKLEEIIQLHGAENIPFIRMEASTNLIGGQPFSIQNLRDVRSIADKYKI
                     RLVLDASLIGENAFLIKQREEEFKNKSLGEILKIMTELADLVYFSARKLSSSRGGGIC
                     TDSNEIYHELEALVPLFEGFLTYGGMSVREMEAMAVGLYETLDETIICQSPNFIKYLV
                     AALDSIGIPVIKPAGVLGVHLDAMQICAHIPQKEYPAGALAAALFLISGIRGMERGSI
                     SNQRDEYGNETYADMELLRLAVPRRVFTLSQIKYVEDRVNWLYDNRQLIGGLRFVYEP
                     PVLRFFMGGLEPVSDWPQKLMAKFKDDFGESL"
     gene            complement(6032..8542)
                     /gene="feoB"
                     /locus_tag="PLZ54_06455"
     CDS             complement(6032..8542)
                     /gene="feoB"
                     /locus_tag="PLZ54_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOJ66603.1"
                     /translation="MQIKTTVLADLEPGQEAVITKIMGHGSFRRRLTEMGFVKGKKIK
                     VIKKAPLQDPIEYEIMGYKVSLRQSEAELVEVMLMDEAQEWLNNQKFEGTFIDNEEKN
                     IPKEEILKHSLLKKEKVIEVALVGNPNSGKTTLFNYASGAHERVGNYAGVTVDSKEAI
                     LKKNGYVFKIVDLPGTYSINEYTPEELFVRNHLTEKMPDVVINVVDASNLERNLFLTT
                     QLIDMDIKVVMALNMFDELEKKGIHFDYRAFGKMLGIPVVPTIASKGKGIDELFKKVI
                     EVYEDADPISRHIHINYGTDIEKAIKEIQDVIWKEPTITDKFSSRYLAIKLLEGDKTT
                     MSQLEKYNNYKEVIKTAEKVRQELEKEYGETAETIITEAKYGFIDGALKETLKLPKKN
                     RRETTHELDDLLTHKIWGYPIFLFFMWLMFEATFKLGNYPMEWIDNGVTLLGNWIENV
                     MPEGALRDLIVQGIIGGVGGVIIFLPNIMILFFFISIMEDTGYMARASFIMDKLMHKI
                     GLHGKSFVPLVMGFGCNVPAIMATRTLKNRKDRILTMIIIPFMSCSARLPVYLLLISA
                     IFYKHQGLILFSLYLIGIMVAVITALLVKKIAFKKEEAPFVMELPPYRIPTLKNTTLH
                     MWDKSKEYLKKIGNVILLVSIIIWALGYFPHNKSISEHYDTQIAKVQQLPLDKEEKET
                     KILTLEVAKESALQEQSYLGQLGHFIQPAMYPLGFDWKMGVSLLAGLSAKEVVVSTMG
                     ILYQAERGADENSASLQEKIQQQVHTSGPLIGQKVFTPITAFAFMIFILIYFPCIAVI
                     SAIKKEGGWQWAVFSVVYNTSFAWLAAFAVYQIGILIV"
     gene            complement(8552..9679)
                     /locus_tag="PLZ54_06460"
     CDS             complement(8552..9679)
                     /locus_tag="PLZ54_06460"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOJ66604.1"
                     /translation="MAKFWGRNKATFPLTMLLSYVVSFMQAQTETTKPLQLSVSYTGE
                     EAGNFSGGIKRGSTYLGLVSLKAELNTQYAGWWEGGTFMAEGMNTHGGEPAANLTGDF
                     HGVSNIEAGNHTFLNNLWFAQEIGKIKLTAGLQNMDGNFASTEYGSAFINSSFGIPST
                     FAYNIPSPIFPFTALGLRLEWNILENLWLKTAIYDGQPDDFESHPHRLNWEIGQKEGF
                     LSISEIEWGKSLLKNQNGSYKIGFYYHQSTDSLNIKQNNKGAYLIVDQQISKQTAAFA
                     QLAISPKNKNQHYLYAGAGINLKGILRQNSDDLLGLGVAYAALKNFDKKGETAFELTY
                     QYCFNEHFALQPDLQYILHPAGTEEKINNAFAGLLRFYINF"
     gene            10027..>10214
                     /locus_tag="PLZ54_06465"
     CDS             10027..>10214
                     /locus_tag="PLZ54_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66605.1"
                     /translation="MKPTCIAYTNTNEYNIQTCGFSIRPLKNKLYSDEKTILFVLAIF
                     VSCSLMKADPAKKVNVTY"
BASE COUNT         2978 a         1932 c         1957 g         3347 t
ORIGIN      
        1 aaaagtcatc accttgtcaa agaactactt tatttcggta ctaaagccgc ttaatcttaa
       61 tttttttcta acagactatt gatattgcaa attattgtga aatatcgaaa caacaaaaaa
      121 tttaatgcct ctcttcaaaa aaatcttatt ttttctcttt tttgaaaaaa tcaggtttta
      181 agaggggatt caatcatcat tttgtcgaac ttttttcaat gcaaaaaacg aaccatttat
      241 aatctcaatt gtttttaata aatttcaatt tacaagtaat ggaacatcat catcaacata
      301 cgcatacaca tggagagagc aacataaaaa tggctttttt tcttaatttc ggatttaccg
      361 tttttgaaat ttttggcggg attttcacca acagtattgc tattatttcc gatgccatac
      421 acgatatggg cgatagcctt tcattgggtt tagcatggta tttccaaaaa aaatccacaa
      481 aagaaagtga taaaaattac acctacggat acaaaagatt ttctttattg ggtgccatta
      541 tcaattccat tattttgatt acgggtagca tttttgtcct ttacgaagcg ataccgcgtc
      601 ttttacatcc cgaaacgacc aaaacgcaag gaatgattgt ttttgctgtg ataggaattg
      661 tagtaaacgg gttggctgtt cttcgtctca aaaaaggaag ttctatcaac gagaaggttg
      721 tttctctcca tatgctggaa gatgtgctgg gatgggctgc tattctggtg ggttctctga
      781 ttatgtattt ctttgatttg ccggttattg atcctgtttt atccgttttg attacaattt
      841 acattttgtt tcatgttttt cgcaacgtaa aggaaagttt gcacattctt cttcagggtt
      901 cgccaacgga tgtcgacatg gaaaaaattt cacaattgct tgtgtcgatt gaaaatgtgg
      961 atgaaattca cgatttgcat atttggtcta ttgatggaaa ttataatatt cttactgttc
     1021 atgtcgtttt gaaacaacca gttacaatag aaaaagctgt agaaataaag aataaaattc
     1081 gctcagaatt gcttcaacaa ggtattgaac atgccactat cgaatttgaa acaacggaag
     1141 aaaactgctc tttacagcaa tgttgttgat tttcagttgt ttgttaaaaa tagaatacaa
     1201 aaatcacttt ttgtgataat ttaccaaagt tttaattaag ccccaaatat attgatgaaa
     1261 aaagtttttg aataaaagtt gttaaattga tttcataatt cgcatgattt acaaatgttt
     1321 actgtattag gttgtatgtt ggccggtatg gtagtcggct ttatttttcg aaaaaaacat
     1381 ttcaaaatta ttcaatcggt tttgtttgtt ttaatctggc tgttgctttt cttattggga
     1441 gcagaaattg gcagtaatcc tgcagttatt cgtcaaacgg gaaagctcgg ctttgatgca
     1501 ctgttaattg gaacggccgg aactttggga agtatactcg gagcagattt gttgtggaaa
     1561 tggataaaac cggataaatc gacccatgaa aagtagcctg attattttgt ctttctttgc
     1621 aacaggcttg cttttaggca ttttcaggtt gcttcccgaa actctcttta acgaagaaat
     1681 cagcttttat gtgctttgct gtttgatgtt taccgttgga ttcagtatcg gacagcaagg
     1741 caattttgtc cgtcaatttc gtcgcttgaa cccacgcatt gtctttttac ctctgtttac
     1801 cattgccggt acgtggttgg gtgcgctggc agtcggtttc ttcattaacg gcttttctct
     1861 gttcgattgt ttggcagcaa gttcgggatt tggttattat tcgctttcca gtatcctgat
     1921 cactcgctac aaaggcgtag agttggggac tattgctttg ttgtccaata tcgtgcgtga
     1981 aattttcact ttgcttttcg ctcctctcat ggtaaaatat tttggaaaac tttctcccat
     2041 ttcttcaggt ggcgctacta ctatggatac cactttcccg gtgattgttc aaagtgccgg
     2101 aaaggattat gcgttggttt ccatttatca cggatttatc acggatttat catggacttt
     2161 tccgtccctt ttctggtaac tttctggtgc agtctctgaa aataaaaaaa ttctaatttt
     2221 ttctccttta ttacggaaaa aattccgtaa ttttgcaacg tttttcaatc tatcgctcat
     2281 tcaaaaaaat tataacgttt atggcaaaag aattcaaata tcaggaactt tttcagttag
     2341 gaaaagacaa aaccgagtat tatttactat cgaaggattt tgtttcgacg gcagagtttg
     2401 aaggcaagga aattttaaaa gttcagccgg aaggtttgac ttttcttgcc cgtactgctt
     2461 ttcgcgactg ttcttttctg cttcgtcccg aacatcaaaa acaggttgcg gccatccttt
     2521 ccgaccccga agccagcgaa aacgataaat acgtagcatt aaccatgctt cgcaatgcag
     2581 agatttctgc aaaaggtatt cttcctttct gccaggatac cggtacggca actatttatg
     2641 ccaagaaagg cagtaacgtt ttcactgatg gaacggatga agaagcttta tccaaaggag
     2701 tttatgaaac ttatacgcag gaaaatctgc gttattcgca aaatgctcca cttaccatgt
     2761 acgacgaagt gaataccggt accaatcttc ccgcccagat tgatattatg gtcggtcatg
     2821 gtaacgaata cgaatttctt tttatcgcaa aagggggcgg ttcggccaac aaatcatttc
     2881 tgtttcagga aacgaaagct ttgctgcaac ccgacacgtt ggaaaaattt cttaccgaaa
     2941 aattgaaatt attgggcacg tcggcttgtc caccctatca tattgcattt gttatcggcg
     3001 gcacatctgc cgatgcttgc atgaaaacgg ttaaactcgc tgcagccaag tattacgacg
     3061 aactgccaac tacaggaaat gagttgggaa gagctttcag ggacgtggaa ctggaagaaa
     3121 aaatcctgaa agcagctcaa aatttgggta ttggggctca atttggagga aaatattttg
     3181 ctcacgatgt gcgaattatc cgactcccgc gtcatggagc ttcctgcttt gtaggattgg
     3241 cggtatcttg ttctgccgat cgcaacatta aggcaaagat aaataaagat ggtatctggc
     3301 tggaaaaact ggaggataat cccggtaaat atattcctga agaatatcgc aatacaggtg
     3361 aaggaaacgc agtgaaaatc gatttgaatc gtccgatgaa tgaaattttg tccacattga
     3421 caaaatatcc ggtttccaca cgactttctc tttcgggaac aatggtagtg gccagagata
     3481 ttgctcatgc ccgcataaag gaaatgctcg atgcagggaa agatttgcca caatatatga
     3541 aagatcatcc ggtgtactat gccggccccg ccaaaacgcc caaaggaatg ccttccggtt
     3601 cgtttggccc tactacagcc ggtagaatgg atacttatgt agatttgttt caggaacatg
     3661 gcggcagtat ggtgatgata gccaaaggca accgcagtca gcaagtggta gatgcctgta
     3721 aaaaacatgg cggtttttat ttgggttcta ttggtggccc ggctgccatt ctcgctcaaa
     3781 attccataaa aaaagttgaa tgtatcgatt tcccggaact tggaatggaa gctgtttgga
     3841 aaatcgaggt agaagatttt cccgccttta ttctgatcga cgacaaggga aacgacttct
     3901 tcaaactgat taagccaacc atgacttgtt gatcagcttc gattttttta atgtcatttt
     3961 tccttttatt gaagggcagt tcgaaaaagc tgcccttcaa tatttacaaa aaattactat
     4021 aatttaatca aaactctact aaagaataag tacaatttta ctcaataaat accaattaat
     4081 taattacttt tgtaatgaat attgtattcg atggtttatt tagaaaaaaa tattttcatc
     4141 caacgaaaga aataatatcc cgataactac tttaattaaa aaattttaca tcattataat
     4201 aacttcaaaa tgtagtatta actttttatt tttttattat gcaagaaatc aaattttaca
     4261 aaggcgaaaa cattcccctt gaattacaca aagttagagt agtacagaaa ttacatcttg
     4321 tccccattga aaggcgattg gaagcattgc aggaaggtgg atacaataca ttcagattga
     4381 gtactaaaga cgtttttctg gatatgttga ccgatagcgg caccaatgct atgagtgata
     4441 atcaaatggc tggcatgatg caggccgatg atgcttatgc cggatcacaa agtttttaca
     4501 gacttcaaaa agctgttcag gatgttttgg gtaaaaaata ttatttgcct gttcatcagg
     4561 gtcgtgctgc agaaaatatt atttcttatg cctatgtaaa aaaaggaagc ataataccga
     4621 tgaactacca ttttacaaca gctatggccc acatcacgca atatggcgga aaaatcgccg
     4681 aactactcta tgatgaagcc tatgtaattg attctgagca tcctttcaaa ggaaatatga
     4741 atctcgaaaa gctggaagaa atcattcaac tgcacggagc tgaaaatata ccgtttattc
     4801 gtatggaagc ttctacaaat ctgattggcg gacaaccatt ctctatacaa aatttacgtg
     4861 atgtaaggtc aatagccgat aaatataaaa tccgtctggt actcgatgcc agcttgattg
     4921 gagaaaatgc ttttttgatt aaacaacggg aagaagagtt taaaaataaa tcattgggtg
     4981 aaattctgaa aattatgacc gaattagctg acttggttta tttttcggcg cgaaaactca
     5041 gttcatcgcg gggtggtgga atttgtaccg acagtaatga aatttatcac gaattggaag
     5101 ctctggttcc actttttgaa ggatttctta cttatggagg tatgtcggta cgcgaaatgg
     5161 aagcgatggc cgttggacta tatgaaacat tggatgaaac aattatctgc caaagtccaa
     5221 attttataaa atatcttgtc gccgcactcg actccatagg cattcctgtc ataaaaccgg
     5281 caggtgtgct tggtgttcat ttggatgcca tgcagatatg cgctcacatt ccacaaaagg
     5341 aatatcctgc aggtgcactt gctgcagccc tctttctgat ttccggtatt cgaggaatgg
     5401 aacgcggttc tatttcgaac caacgtgatg aatacggtaa tgagacttac gccgacatgg
     5461 aattgctgcg tttagccgtt ccacgccgtg tttttacact ttcacaaatt aaatatgttg
     5521 aagaccgagt aaattggttg tacgataatc gacaacttat cggaggactt cgatttgttt
     5581 atgaaccacc tgttctgcgc ttctttatgg gaggacttga accggtaagc gattggccac
     5641 aaaaacttat ggcaaaattt aaggatgatt ttggcgagag tttataattt gaaaaatgag
     5701 ggagggaaat ttttctctct cttattttta cggatgtttt tggtcaacta attgattttt
     5761 tgttaaaaaa cttgttttgt tgaacgaaaa attttctttt tccccggaac aacatttatc
     5821 gggggcatgg aaaagattat ctttactgtt tttcagaatt tcttgtttta tttcgcagcc
     5881 gttgcatcca ctacagggag aattcttttt gctgcgaatt gtttttacta tttttaaggc
     5941 cgaaattact atcgctccta caataattag gtaggttata atggtttgtg tcatggcatg
     6001 aataaattat aaaattacgt ttttattaat atcatacaat caggattcca atttggtaaa
     6061 ctgcaaaagc cgccagccag gcaaaagaag tattgtacac aacggagaat actgcccatt
     6121 gccagccacc ttctttttta atagccgata tgacagcaat acaggggaaa tatatcagga
     6181 taaaaatcat aaaggcaaat gccgttatgg gcgtaaaaac tttttgacct atcaacggtc
     6241 ctgaagtatg cacctgttgt tgaatctttt cctgaagcga agcagaattt tcatcggctc
     6301 ctctttcggc ttgatacaaa attcccattg tgctcaccac tacctctttt gctgataatc
     6361 cggccagtaa actgacgccc attttccagt cgaaacccag cggatacatg gccggctgaa
     6421 taaaatgccc caactgaccg agataagatt gttcctgcaa ggctgattct tttgctactt
     6481 ccaaagtcaa aatttttgtt tctttttctt ctttatcaag aggaagttgt tgaacttttg
     6541 caatttgtgt atcgtaatgt tcgctgatcg atttgttgtg aggaaaatat cccaacgccc
     6601 aaataatgat ggaaacaagt aaaatgacgt tacctatttt tttcaggtat tctttacttt
     6661 tgtcccacat gtgcagggtg gtgtttttta atgtgggtat gcggtaagga ggcaattcca
     6721 tcacaaaagg cgcttcttct tttttaaaag caattttctt taccaacaat gctgtaatga
     6781 cggctaccat gattccaatc aagtaaagcg aaaacaggat caagccttga tgtttgtaaa
     6841 aaatagccga aatgagcagc agataaaccg gtagtcgggc gctgcacgac atgaaaggaa
     6901 taataatcat ggttagaatt cggtcttttc tgtttttcag cgttcgggta gccataatgg
     6961 ccgggacatt acagccaaaa cccattacca acggaacaaa cgatttcccg tgtaacccga
     7021 ttttgtgcat gagtttatcc ataataaaag atgctctggc catataaccg gtgtcctcca
     7081 taatcgatat aaagaaaaac aaaatcataa tgttgggaag aaatatgatt acaccaccca
     7141 caccaccaat gataccctga acaatcaaat cccgcaaggc tccttcgggc ataacatttt
     7201 ctatccagtt tcccagcagc gtgaccccgt tgtcgatcca ttccattgga tagtttccca
     7261 gtttgaaagt ggcttcaaac atcaaccaca tgaagaatag aaaaatggga tagccccata
     7321 ttttgtgagt cagcaggtca tccagttcgt gcgtagtttc tcgtcgattt tttttgggaa
     7381 gtttgagcgt ttctttcaac gctccgtcaa taaatccgta ttttgcttca gtaattatgg
     7441 tttctgccgt ttctccgtat tctttttcaa gttcttgtct tactttttct gcagttttta
     7501 ttacttcctt gtaattgttg tatttttcga gttgggacat agtggtttta tcaccctcca
     7561 gtaatttgat agccaagtat cgtgaagaga atttgtcggt tatggtgggc tctttccaaa
     7621 ttacatcctg aatttccttg atagcttttt caatgtctgt accataattg atgtgaatgt
     7681 gtcgggaaat gggatcagca tcttcgtaaa cttctattac ctttttaaat aattcatcaa
     7741 tgccttttcc tttggatgcg atggttggca ccacaggaat tcccagcatt ttcccgaatg
     7801 cgcggtagtc gaaatgtatt ccttttttct ccagttcgtc aaacatgttg agcgccatta
     7861 ccacttttat gtccatgtcg ataagttggg tagtgagaaa aagatttctt tccagattag
     7921 aagcatccac aacgttgatc acgacgtcgg gcatcttttc ggtgagatga tttcttacga
     7981 aaagttcttc aggcgtatat tcgttgatgg aataagtccc aggcagatca acaattttaa
     8041 aaacatatcc gttttttttc agtatggctt cttttgaatc tacagttact ccggcataat
     8101 ttcccactcg ttcgtgtgct ccggatgcgt aattgaaaag tgtcgttttt cctgaatttg
     8161 gattccctac caaagccact tcaatcactt tttccttttt aagtagcgaa tgtttgagaa
     8221 tttcttcttt aggaatattt ttttcttcgt tatcaatgaa agtcccttca aatttttgat
     8281 tgttcaacca ttcttgcgct tcgtccatca gcattacttc aaccaattcg gcttccgatt
     8341 gacgcaaaga aaccttgtaa cccattattt cgtactcaat gggatcctgc aagggtgctt
     8401 ttttgataac ctttattttt ttccctttga caaaacccat ttctgtcagt cgtcttcgga
     8461 atgatccatg tcccattatt tttgtgatca cggcttcctg accgggttcc aaatcagcca
     8521 atacggttgt ttttatttgc atgacgagtg tttaaaaatt aatataaaaa cgcagcaatc
     8581 cagcaaaagc attatttatt ttttcttccg tgcctgccgg atgaagtata tattgcaagt
     8641 cgggctggag agcgaaatgt tcgttgaaac agtactgata agtgagttca aaagctgttt
     8701 ctcccttttt gtcgaaattt ttcaatgctg cataagcaac tcctaatccc aataaatcgt
     8761 cggaattttg tcttaaaatt cctttcaggt tgattccggc tccggcatac aggtaatgtt
     8821 gatttttatt ttttgggctg attgccaatt gagcaaaagc tgcagtttgc ttactgattt
     8881 gctgatcgac aatcagatat gcgcctttgt tgttttgttt tatgtttaaa ctgtctgttg
     8941 actgatgata ataaaatcca attttgtaag aaccattttg atttttcagc agactttttc
     9001 cccattctat ttcactgata gaaaggaatc cttctttttg acctatttcc caattcaaac
     9061 ggtgaggatg ggattcgaaa tcatcaggtt ggccatcgta aatggctgtt ttcaaccata
     9121 aattttccaa aatattccat tccaagcgga gtccgagtgc tgtaaatggg aaaatgggac
     9181 ttggaatatt gtaagcaaaa gtagaaggaa tgccaaaaga actgttgatg aaagcactgc
     9241 catattcggt ggaggcaaag tttccatcca tattttgcag tccggcagtg agtttaattt
     9301 ttcctatttc ctgagcaaac caaagattgt tcaaaaaggt atgattcccg gcttcaatat
     9361 tcgaaacgcc atgaaagtcg ccggtcaaat ttgctgctgg ctctccgcca tgcgtattca
     9421 ttccttctgc cataaaggtt ccgccttccc accaaccggc gtactgtgta ttcaattcag
     9481 ctttcagcga taccagtccg agatacgtgc tgccacgctt tattcctcca ctgaaatttc
     9541 ctgcttcttc tcccgtatat gaaaccgata actgcaaagg ttttgtcgtc tcggtttgag
     9601 cctgcataaa tgaaacgaca taactcagca gcattgtcag cgggaaagtt gccttatttc
     9661 taccccaaaa ttttgccatt aaccattgta tccgattttg ttctgaattt tttcccatta
     9721 atcttaataa aatttctttc tgtttctact cttgttttgg agacgcaaag ttacgcaatt
     9781 tagattgatt ctaaataata atatccaaaa aaatttcaat aaatttgaaa tttcctaata
     9841 atcattcaaa tatcaattca ttaaccattt ttagacaact tgatattgat ttgaataatt
     9901 tatgagaatc agtttcaact gctgactcgc aggctaactc gcataaatta tttctttaag
     9961 taggttggag atttttcagc acttacaaaa ccaaacattt tataaattat aaaaattgat
    10021 ggagatatga aacccacatg tattgcatac acaaacacga atgaatataa tattcagaca
    10081 tgtgggttct ccatcagacc tttaaaaaat aaattatatt ctgatgaaaa aacaatttta
    10141 tttgtattgg caatatttgt ttcctgttca ttgatgaagg ccgatccggc aaaaaaagtc
    10201 aatgtaactt atca
//
LOCUS       DAOSCH010000075        10202 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4321_length_10202_cov_5.373411, whole genome shotgun sequence.
ACCESSION   DAOSCH010000075 DAOSCH010000000
VERSION     DAOSCH010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 10202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10202
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4321_length_10202_cov_5.373411"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            456..1136
                     /locus_tag="PLZ54_06470"
     CDS             456..1136
                     /locus_tag="PLZ54_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HOJ66606.1"
                     /translation="MKKQVSIFFMLCGLLFTVCLITANIVEQKLIALGPIEATAGLLI
                     FPVSYIVNDLIAEVWGYRKARLIIWSGFAMNFLAVVIFRLSMLIPPSHNFQNQEAFAL
                     IMGNTLRITIASFVAFLVGSFLNAFVMSKMKILQHGRHFSVRAIVSTLVGEGCDSLVF
                     FSLAFAGVIPTRDLFLLMLTQTAMKTGYEIVVLPLTNFVVNWMKKKEETDVFDYDISY
                     SPIKLKEI"
     gene            1245..2381
                     /gene="nspC"
                     /locus_tag="PLZ54_06475"
     CDS             1245..2381
                     /gene="nspC"
                     /locus_tag="PLZ54_06475"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HOJ66607.1"
                     /translation="MIDFSKIPSPCFVLDEHALRRNLELIRYVKEESGAEIILAFKAF
                     AMWSVFPIIKEYGITKSAASSLAEARLAYEEMGNKAHTYSPVFSQKEFSEILSYSSHI
                     TFNSLLQFHQFHSQAKAKGVSCGLRINPEYSEVKTDLYNPCSPGSRLGVTFDLLGEKL
                     PDGIEGLHFHNLCESNSYELEKTLEEVKKKFGKYFSQVQWINMGGGHLMTKEGYDIEH
                     LISIIRSFKKSYSHLKVILEPGSAFAWQTGVLLATVMDIVENHGIKTAMLDASFACHM
                     PDCLEMPYKPAIIGATDEQKGKPTYRMGGNSCLAGDFFGNWSFDNELKIGDKIIFEDM
                     MHYTMVKTTMFNGVTHPSIGIWTKENKFKLIKKFGYEDYKSRLS"
     gene            complement(3276..3351)
                     /locus_tag="PLZ54_06480"
     tRNA            complement(3276..3351)
                     /locus_tag="PLZ54_06480"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3313..3315),aa:Pro,seq:ggg)
     gene            complement(3470..6715)
                     /gene="carB"
                     /locus_tag="PLZ54_06485"
     CDS             complement(3470..6715)
                     /gene="carB"
                     /locus_tag="PLZ54_06485"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HOJ66608.1"
                     /translation="MKHTYQSPKKGEIKKVLVLGSGALKIGEAGEFDYSGSQALKALK
                     EENIQTVLINPNIATVQTSEGIADKVYFLPVTPYFVEKVIEKERPDGIFLSFGGQTAL
                     NCGVALYQQGVFEKYNVTVLGTPVQSIIDTEDRQLFNQKLSEIDVKYIKSHAVTNVND
                     AVSAAHDLGYPVIVRAAYALGGLGSGFCNNDEELHALVEKAFSYSPQVLVEKSLKGWK
                     EVEYEVVRDRYDNCITVCNMENFDPLGIHTGESIVVAPSQTLTNSEYHKLRELSIRII
                     RHIGIIGECNIQYALDPFSEDYRVIEVNARLSRSSALASKATGYPLAFIATKLGLGYG
                     LPELKNSVTKTTSAFFEPALDYIVCKIPRWDLSKFNGVSHQLGSSMKSVGEVMAIGRC
                     FEEVFQKGLRMIGLGMHGFVANREYFSDSLETALSQPTDKRVFYLAQALHEGYSIDKL
                     YELTKIDRWFLFKLQNIVRLEHELQEYDSLEKLPNELLLEAKKAGFSDFQVTRLITKC
                     LPEEIEEKMKLTRVHRQRHNILPSVKQIDTLAAEYPALTNYLYLTYNGCGNDVQHEDD
                     QRSVIVLGSGAYRIGSSVEFDWCGVNALTTIRKEGYRSVMINYNPETVSTDYDMCDRL
                     YFDELTFERVMDIIEMEMSKGVVVSTGGQIPNNLAMKLANAKVNILGTQAEFIDMAED
                     RHKFSAMLDQLGIDQPRWKELTTFEEVNEFVERIGYPVLVRPSYVLSGAAMNVCYDAS
                     QLEEFLKLATNVSKQHPVVISEFLERCKEIEIDAVADKGEIIVYAISEHIEFAGVHSG
                     DATTQFPPQKIYVETIRRIKNIARQIARSLNISGPFNIQFLARDNYIKVIECNLRSSR
                     SFPFVSKVLKINFIEIATKIMLGIPVEKPEKSAFDLDYVGVKASQFSFARLQQADPIL
                     GVDMASTGEVGCLGDDSSEALLKSLLSVGITIPKKNILISSGEPKSKVDLLDACRLLY
                     RKGYQIYATRGTHQFLMDNGVASIPVQWPDEEGEYNVKDMIAAKKFDLVINIPKNVTR
                     RELANDYTIRRSAIDFNIPLLTNARLASAFIFAFCNLTMEDVKIKSWDEYH"
     gene            complement(6776..7840)
                     /gene="carA"
                     /locus_tag="PLZ54_06490"
                     /pseudo
     CDS             complement(6776..7840)
                     /gene="carA"
                     /locus_tag="PLZ54_06490"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
     gene            8279..9475
                     /locus_tag="PLZ54_06495"
     CDS             8279..9475
                     /locus_tag="PLZ54_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOJ66609.1"
                     /translation="MKRKIGCLLIVLCILSNISAQDNFFHYASAVWIENNGNGQFYNI
                     YSISTTTSGHTYTAQENAIEQGGGNWQSALFQDRDFGSHAANSHSLIFRGAEVKTYKK
                     NPGNVCSAKVFYVVYPQGQRPVSPTFTEHNIAWFDNCDLSQYKFPDLPGDGPCNESGY
                     QKWQSWASGGVGHLTENTVLDLTQMKEGKYTLELYYQVNGSNTVPNECSETVYINNGG
                     NNYKANFTVCPVISLASVVQPSSCGGNGQIILTTAGIENGIYNQFYYTDASDVIHSFG
                     NVQLINNSATLTVPAGVYKDIRYVSSVSGCSPSIAGNVTLQDPPLPQITGITQSNVQC
                     FGDSSGWIEVSVSGGSGQYFFSRDGGINYTSESHTSPYKFENLPAGNYNIQVKDTKGC
                     VANNCP"
     gene            9552..10145
                     /locus_tag="PLZ54_06500"
     CDS             9552..10145
                     /locus_tag="PLZ54_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprB repeat-containing protein"
                     /protein_id="HOJ66610.1"
                     /translation="MKKNYFLLSLIVVLTFFAPAIKAQSSVCPVIITQPNELKIDNID
                     KKTYNGADLSCSNAADGQITVTASGGTTPYQYSKDNGATYQSSNVFSGLSAGIYQIVV
                     KDANGCTTAPQQINITAPAALTMSIEQWDVTCFGDNNGRITVTVSGGSGKYRFSKDGG
                     SKFTPFQAGNEYTFENLVAGTYNIVAEDENGCSAACP"
BASE COUNT         3012 a         1952 c         1988 g         3250 t
ORIGIN      
        1 aataatttat tgtcaatgaa tgaattagta gagacgtagc atgctacgtc tctactaaac
       61 aggttgaatt acaattgttt atttatagac acatgttttt ggttttaatg aatatcattg
      121 tttgtcagac cttaatttgc taattaacct tagtaacaag gcaaaacttc cttcccatct
      181 aaaaccttat tagcttattt ttaactgcga aatagcctgg aggcaatacc gaacaatatt
      241 ttttattttt ataactcgtt gattatgaat taatttgtag agacgtagca tactgcgtct
      301 ctactgaaca atttgaatat tgtattttta aaaaagtaca cttttgggga ctacctgctt
      361 ttgtttttta aagaaataag cgttaatttt gcaaaaagtt tctgctgctt ttcttgcaga
      421 acgtgtgctt gaacaccacg taaaaaacaa gacatatgaa aaaacaagtt tccattttct
      481 ttatgttgtg cggtttgctg tttaccgtct gtcttattac cgctaacatt gttgaacaaa
      541 aactgattgc cttaggccct atcgaagcca ctgccggttt gcttattttc cctgtttcct
      601 atattgtgaa cgatttgatt gccgaagttt ggggctatcg gaaagcacgt ttgattattt
      661 ggagcggatt tgccatgaat tttttggctg tggtcatttt caggttaagc atgcttattc
      721 caccttcaca taattttcaa aatcaggaag cttttgcttt gattatggga aatacgctac
      781 gcattacgat tgcgagcttt gttgcttttc tcgttggttc ttttctgaat gcttttgtta
      841 tgagcaagat gaaaatttta caacatggtc gtcatttctc tgttcgagct atcgtttcca
      901 cgcttgtggg agaaggatgc gactcgctgg tgtttttttc gctggcattt gccggtgtca
      961 tcccaacccg cgacttgttt ctcttaatgc ttactcagac ggctatgaaa acgggatacg
     1021 aaattgttgt tttgcccctt actaattttg tggtcaattg gatgaagaaa aaggaggaaa
     1081 cagacgtatt tgactatgat atttcttaca gtccgattaa gctgaaagaa atataaaaat
     1141 aaaattgtgt agaagttgtt ctcaaaccat ctttgcatca aactgtatct atgtaaatag
     1201 aaaataatcc tgtaactttg taaaaaaatt aacgatcaca ctgcatgata gatttttcaa
     1261 aaattccttc tccctgcttt gttttggatg agcatgcact tcgacgaaat cttgaattaa
     1321 tacgatatgt gaaggaagaa tcgggagcgg aaataatatt ggcttttaag gcttttgcca
     1381 tgtggagcgt gttccccatt attaaggaat acggtattac taaatcggct gccagttctc
     1441 tggcagaagc cagattggca tacgaagaaa tgggaaataa ggctcatact tattctccag
     1501 ttttttccca aaaagaattt tccgaaattt tatcttacag tagccatata acttttaatt
     1561 ccctgctgca gtttcatcaa tttcactctc aggctaaagc aaaaggtgtg tcttgtgggt
     1621 tacgaataaa tccggagtat tccgaagtaa aaaccgattt atacaatcct tgttcgccag
     1681 ggtcaagact cggagtaact ttcgatttac tgggggaaaa actaccggat ggaatagaag
     1741 gcttgcattt tcacaactta tgtgaatcta attcctatga attggagaaa acgctcgaag
     1801 aagtaaagaa aaaatttggc aaatattttt cgcaggtaca atggatcaat atgggtggtg
     1861 gacatttgat gacaaaagaa ggttacgata ttgaacattt gatttcaatt attcgttcct
     1921 tcaaaaaatc gtattcccat ttgaaagtga ttcttgagcc cggaagtgcc tttgcctggc
     1981 aaacaggagt tctccttgcc acagtaatgg atattgtcga aaatcacgga ataaaaaccg
     2041 caatgctgga tgcttctttt gcctgtcaca tgcccgattg tttggaaatg ccttataaac
     2101 ctgcaattat tggagccaca gatgagcaaa aggggaaacc tacctatcgt atgggaggga
     2161 acagttgtct ggcaggtgat ttttttggaa attggtcatt cgacaacgaa ttgaaaattg
     2221 gcgacaaaat tattttcgaa gatatgatgc attatacaat ggtgaaaacc actatgttta
     2281 atggagttac acatcccagc atcgggatat ggacgaaaga aaataaattt aagcttataa
     2341 aaaaatttgg atatgaggac tataagagtc ggttgtcgta aaatatgatt gcctaagttt
     2401 cttaccatat cagtatctgc caaaatatat tctttgtctt agtacatgac aaaaaaattt
     2461 ttaaaaacat ttatattttt tattttcaga tatttaataa aggggctttg ttccaaactt
     2521 cacttacttt tttgtcttga agcaaaaaag taagaaaaaa aattaactta gttgaagtag
     2581 ctacgctcaa cataaatcaa ataaatttga ttttgttttc gcttaaacgt tcctttcaac
     2641 actgtgccca cttcactcaa aaaacttacg ttcgaaaagc tgaaatcgtt caaactcatt
     2701 ccaacgaaat acgtcagaac tggtactgaa gtattatatg gaaattttca aaaatctttt
     2761 tgaatatttt tttacccttt tttatctcaa tgtctttaaa aactaattct ctttgagaaa
     2821 cagagggggg ctcttcaatt attttttcaa caaaaggttt atcaattttc agtttaaaaa
     2881 tatatagata cccaatttat aaggagttaa tcaatagttg ttcaaaaaaa tttttatttt
     2941 taatattccc ccttaattct tttttcgatc caattttctg cctgtatgtt cacatcgaac
     3001 ggaattttaa aaaactaacc gtgcgtaacg gaaaatttca aaggataaga accctttttt
     3061 ttatactcct tttgtcgagg tattattttt cagaagccaa aagtgtgttt ataaatttgc
     3121 agtagatttg cagtaaaatt ggtcaaaaat cggagaaaac ttttagaatt tgggaaaaag
     3181 tccaacttgt ttttacaata atattgaatt aaaaatacaa aaaagccttt gaaacaaata
     3241 tattactatt tgattatcaa agactttata ttttgtcggg atgactggat tcgaaccagc
     3301 gaccacacgc cccccagacg cgtactctaa accggactga gctacatccc gaatggttga
     3361 aaacggaata caaaattaag caattttccc tgtatttgaa aaatgttttt ctgcttattt
     3421 tgaactgttc ttttccctat ttccttaatt gcttgaaaat gaaaatattt taatgatatt
     3481 catcccaact ttttatttta acatcttcca ttgtcaggtt gcagaaagca aaaataaagg
     3541 cacttgccag ccgtgcattg gtgagcagag gaatattaaa atcaatggca cttcgccgaa
     3601 tggtgtaatc gttagccaat tctctgcgcg taacattttt tggaatattg ataaccaaat
     3661 cgaatttttt ggctgcaatc atgtctttta cattgtattc accttcttcg tcgggccact
     3721 gaacagggat actcgccacg ccattgtcca tcaaaaactg atgagttccc cgtgtggcat
     3781 aaatttggta tcctttcctg taaagcaatc gacaagcatc caacaaatct actttcgact
     3841 tgggttcgcc tgaagaaatc aaaatgtttt tcttcggaat ggttattcca accgacagta
     3901 aggatttcag gagagcttcc gatgaatcgt cacccaaaca gccaacttca ccggtagaag
     3961 ccatatcgac tcccaaaatt gggtcggctt gctgcaagcg tgcaaaggag aactgcgatg
     4021 cctttacccc tacataatcg agatcgaaag ccgatttttc gggtttttct accggaatac
     4081 ccaacatgat ttttgtggca atttctataa aattgatttt cagtactttc gatacaaacg
     4141 gaaagctgcg cgatgagcgc aaattgcatt ctatcacttt tatatagttg tcgcgagcca
     4201 ggaactgaat attgaatgga cccgaaatat tgagtgagcg agctatctga cgggcaatat
     4261 ttttaattcg gcgaatggtt tccacataaa ttttttgcgg aggaaactgt gttgtagcat
     4321 ctcctgaatg aacacctgca aattcgatat gttccgatat ggcataaaca ataatttctc
     4381 ctttgtcggc aacagcatct atttctattt ccttgcaacg ttccaaaaat tccgaaataa
     4441 ctaccggatg ctgcttggaa acattggtag ccagttttaa aaattcttcc aattgcgaag
     4501 catcgtaaca aacgttcatg gcagcgcccg aaagtacata cgaaggtcga accaaaacgg
     4561 ggtaaccaat tcgttcgaca aattcgttta cttcctcaaa agtggtcaac tctttccagc
     4621 gtggctggtc gataccgagc tggtcgagca tggctgaaaa tttgtgtctg tcttctgcca
     4681 tgtcgataaa ttcggcttgc gtaccaagaa tattgacttt tgcattggca agcttcatgg
     4741 ccagattatt gggaatttgt ccacctgtgg acaccacaac gcctttcgac atttccattt
     4801 cgataatgtc catgacacgt tcaaaggtca gttcatcgaa ataaagccgg tcgcacatgt
     4861 cataatcggt ggaaaccgtt tcgggattat aattaatcat taccgagcga taaccttcct
     4921 tccgaatggt ggtcagtgca ttcacgccac accagtcgaa ttcgacggaa gaaccgattc
     4981 ggtaagctcc cgaccccaat acaataaccg aacgttgatc gtcttcgtgc tgaacatcgt
     5041 tgccgcagcc gttgtaggtt aggtaaagat aattcgtcaa agccggatat tcggctgcca
     5101 gcgtgtcaat ttgcttcacc gatggtaaaa tattgtgtct ctgtcgatga acgcgggtga
     5161 gtttcatttt ttcctcaatt tcttccggca gacatttggt tatcaaacgt gtaacctgaa
     5221 aatcggaaaa gcctgctttt tttgcttcca acagtaattc attcggaagt ttttcgagtg
     5281 aatcatattc ctgaagttca tgttcaagcc ggacgatatt ttgtagctta aaaaggaacc
     5341 agcgatctat ttttgttaat tcgtacagtt tatcgatgga ataaccttca tgtagcgctt
     5401 gagccagata aaatacacgt ttgtcggtag gctgggagag agccgtttca agactgtcgc
     5461 tgaaatattc tctgttggca acaaaaccgt gcatacccaa tccaatcatg cgcaatcctt
     5521 tctgaaagac ttcttcaaaa caacgaccaa tggccatcac ttcaccaacc gatttcatac
     5581 tgctgcccag ttgatgggat acaccgttaa atttgcttaa atcccaacga ggaattttac
     5641 atacgatgta gtcgagtgca ggctcaaaaa atgcggaggt tgttttggta accgaatttt
     5701 ttaattccgg aagtccgtaa cccaagccaa gtttagtagc aataaatgcc agcggataac
     5761 ctgtagcttt cgaagcaagc gctgaagacc gacttaagcg ggcattgact tcaatcaccc
     5821 gataatcttc cgaaaacgga tccagcgcat actgaatgtt gcattcgcct atgataccaa
     5881 tgtgacggat gatgcggatg gaaagttcgc gcagtttgtg atattcgctg ttggtaagcg
     5941 tttgagaagg agctactaca atactttctc cggtgtgaat gcccagcgga tcgaaatttt
     6001 ccatgttgca caccgtaatg cagttgtcgt aacggtctct tactacttcg tattcgactt
     6061 ctttccagcc tttcagcgat ttttctacca aaacctgagg tgaataggaa aaagcttttt
     6121 ctaccaatgc atgcagttct tcatcgttgt tgcaaaaacc gcttcccaaa ccacccaggg
     6181 cataagctgc ccgtacaatt accggataac ccaaatcgtg agctgcggat actgcatcat
     6241 taacattggt aacggcgtgg cttttgatat attttacatc aatttccgaa agtttttgat
     6301 tgaaaagttg acgatcttcg gtatcaataa tcgattgaac cggcgtaccc agcactgtta
     6361 cattgtattt ttcaaaaaca ccctgctgat aaagtgccac gccgcagttg agcgccgtct
     6421 gtccgccaaa ggaaagaaaa attccatcag gacgttcctt ttcgataact ttttctacaa
     6481 agtagggagt aaccggtaaa aaatatactt tatcggcaat tccttctgaa gtttgaacgg
     6541 tagcaatgtt gggattgatg agcaccgttt gaatgttttc ctcttttaat gctttcaaag
     6601 cttgtgaacc ggaatagtcg aattcgccgg cttcaccaat tttcagtgct cccgaaccca
     6661 aaaccagcac tttttttatt tcgccttttt ttggactttg gtaagtgtgc tttataattt
     6721 gttttttctc caattcttct tctatcagtt gaggaatgga tttatttttt tcggtcaggg
     6781 tacgtatgag tacatcaaaa agaaattccg tatcggttgg tcctgaagag gcttcgggat
     6841 gaaattgaca ggaaaaccaa gactttgttt tatgccgtat tccttcgttt gttccatcat
     6901 tcatgtttac aaacaacggt tcccattcgt cgcttaaggt tgaattgtca actgcaaaac
     6961 cgtgattctg ggaagtgata aatgctcttt gtgttcctgc cagttgaacc ggttggttat
     7021 ggcttctgtg tccgtatttc agtttgtata ttttggcgcc tccggccatt gagagcagtt
     7081 ggtttcccat gcaaatcccg aatattggct tgtcggtctg catggccttt cggatattgt
     7141 ttaccgttgc atcacaaaat tccgggttcc ccggtccatt ggaaataaac aatgcatcat
     7201 aatccaattg atgaaaatca taattccacg gcacacgaat aacggtggtg tcgcgtttga
     7261 gcaggcagcg aattatgttg tgtttcactc cgcaatcgac cagcaaaata cggtgcttcc
     7321 cgttgccata agttatcact tctttgcagc tgactttggc aacctgattt tcggtgtcgg
     7381 gatccacaaa atcaatatct tcaccgggaa aaactatttt tcccaacatg gttccttttt
     7441 cacgtaccag tttggtcaat tcgcgggtat ccacaccaaa aattccggga acttcatttt
     7501 ctatcagcca atcgctgagg ctttttccgg cattccaatg actgtaatga aaagaaaaat
     7561 cggaaataat taaagctgat gcctgaattt tttccgattc gtaaaaggtg gataatccgt
     7621 tttgtttttt gtcgatcggg acaccgtaat tacctacgag cgggtaagtg agcgttagta
     7681 actgaccctt gtaggatggg tcggtcaaac tttcgggata gccaaccata gcggtattga
     7741 aaactacttc gcctgcggcc gatttttcgt aaccgaaaga ttttccctcg aagatggttc
     7801 catcttccaa aataaggtgg acaggtttga attgttgcat aaattcctta taaacttttg
     7861 aaaagtagca ggcaaaacta taaaaaaatc cgcctgaaaa aatggtatat ccaaattttt
     7921 gcaaagatac tgatttttct tggttggagc aaaaattggc agaggaaaaa ttggaaatct
     7981 tgatggggaa ggaacggtgt caaattaaca ataactcaag ttatttgcaa acgtttgcaa
     8041 cgattttttt cgatttttct tgcattttgt ttgcatatag gaaaatcatg gctttaattt
     8101 gggaagtttt accttaaatg tagtgtcatg agtcaaaaac acttaccaac ccaaaacaag
     8161 cctgttttta acctgacagc tcatgagtgt tttgcaatga aaatgttgaa ggtaatactt
     8221 ttgaattcaa tttattacta aatgattttt tgttgaacaa acataacttt attttcggat
     8281 gaaacgaaaa attggctgtc ttttgattgt tctttgcatt ttatccaaca taagtgctca
     8341 ggataatttc tttcattatg cctctgccgt gtggatagag aataatggta atggtcaatt
     8401 ttacaacatt tattccattt ccaccactac ttctggtcat acttatactg ctcaagaaaa
     8461 tgccattgaa cagggtggtg gaaattggca atcagcattg tttcaagata gagattttgg
     8521 ctctcatgca gccaattctc attctttgat ttttagaggt gctgaagtca aaacctacaa
     8581 aaaaaatccc gggaatgttt gttctgccaa agttttttat gtagtatatc cgcaaggtca
     8641 acgacccgtt tcccccactt ttaccgaaca taacatagca tggtttgata attgtgattt
     8701 gagtcaatat aaatttcctg atttacccgg agatggccct tgtaatgaat ccggctatca
     8761 gaaatggcaa tcgtgggctt caggtggagt tggacattta actgaaaata ccgtactgga
     8821 tttgactcag atgaaagaag gaaaatatac gctggagctt tattaccagg ttaatggcag
     8881 caacactgtt cccaatgaat gttcagaaac agtttatatc aataacggtg gaaataatta
     8941 caaggccaat tttacggtat gtcctgttat ttcattagct tctgtggtcc agccttcttc
     9001 ctgtggcggg aatggtcaga ttattctcac cactgccggt attgaaaacg gtatttacaa
     9061 ccagttttat tatacggatg cttccgatgt tattcattct tttggaaatg tgcagttgat
     9121 caataattca gcgactttaa ctgtccctgc tggtgtgtat aaagacatta gatatgtatc
     9181 ttctgtctcc gggtgtagcc catcaatagc aggaaatgtt actttacaag atcctccgct
     9241 tcctcaaatt acaggtatca cccaatcgaa tgtccaatgt tttggtgaca gttctggctg
     9301 gatagaagtc agcgtttcgg gcggttcggg tcaatatttt ttctcgagag atgggggtat
     9361 aaattacact tctgagtcac acacaagtcc ttacaaattt gaaaatttgc cggctggaaa
     9421 ttataatatt caggtaaaag acacaaaagg gtgcgtggca aataattgtc cttagtttta
     9481 ttgaaaaatt gtttaaatta aacaaataat cttgtgaaaa aattgacaaa taaccattaa
     9541 atcttttcaa aatgaaaaag aattattttt tactttcctt gatagttgtc ttaacttttt
     9601 ttgcgcctgc tatcaaggca caatctagtg tttgtccggt tatcatcact cagcctaatg
     9661 agttgaaaat tgacaatatt gacaaaaaaa cttataatgg agcagattta agctgttcaa
     9721 atgctgccga cggacaaatt actgtcactg caagtggagg tactactcca tatcaatatt
     9781 ccaaagacaa tggagcaact tatcaatcga gtaatgtatt cagtggattg agtgccggaa
     9841 tctaccaaat tgttgtcaag gatgccaatg gctgtactac tgctccacaa caaatcaaca
     9901 ttacagcacc ggcagcattg acaatgagta ttgaacaatg ggatgtaact tgctttggcg
     9961 ataataatgg aaggataaca gtgactgttt caggcggttc aggtaaatac cgcttttcta
    10021 aggatggtgg aagtaaattt actcctttcc aagccggcaa cgaatatact tttgagaatc
    10081 ttgtggcagg aacttataat atagtggccg aagatgagaa tggatgcagc gctgcttgtc
    10141 cttgaaatct taaataaggg gacggattga acttaatgta acgtgagtcc gatataagaa
    10201 tt
//
LOCUS       DAOSCH010000076         9971 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4448_length_9971_cov_6.619100, whole genome shotgun sequence.
ACCESSION   DAOSCH010000076 DAOSCH010000000
VERSION     DAOSCH010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9971
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4448_length_9971_cov_6.619100"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="PLZ54_06505"
     CDS             <1..638
                     /locus_tag="PLZ54_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOJ66611.1"
                     /translation="LIQESETMDLKNLEEGYQHLSEIFPQYQISMVHGKMKPAEKEAE
                     MQKFVAGKTQIMVATTVIEVGVNVPNASVMIIENAERFGLAQLHQLRGRVGRGADQSY
                     CILLTGYKLSNLTRKRIETMVNTNDGFQIAETDLQLRGPGDLDGTQQSGLPFELKIAN
                     LAQDGKMLEMARKVAMDIIEEDPQLQKKENLILSEQLKKMKTSSLNWSVIS"
     gene            739..1200
                     /gene="ndk"
                     /locus_tag="PLZ54_06510"
     CDS             739..1200
                     /gene="ndk"
                     /locus_tag="PLZ54_06510"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOJ66612.1"
                     /translation="MEKTLVIIKPGAIQRELIGEIITRFERKGLHLCGIKMYQFTDKV
                     VSEHYAHLADRPFFKRIKDSMMVTPVIVCCWEGLDAVRIVRSMSGVTNGREAAPGTIR
                     GDFSMSVQENIVHASDTVENAKVEIDRFFKPEEIFEFKPNNFPFVYANDEV"
     gene            1373..2110
                     /locus_tag="PLZ54_06515"
     CDS             1373..2110
                     /locus_tag="PLZ54_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOJ66613.1"
                     /translation="MLNDTSDDLMENHPADDIYNNIWTSERLNPYHFPVDSLPDSVRI
                     DCSNFILPIYGKVTSKFGRRRYRYHYGIDLKLNVGDSVRCAFEGKVRIIDYEHGGYGH
                     YVVVRHNNGLETVYAHLSKVLVELNQIIGAGEVIALGGNTGRSTGPHLHFEMRYLGNA
                     IDPDNLVDFEKGSIRQFSYLITKKNTFKYQKDLKSLQMARYHVVHKGDTLSLIASRYG
                     TTVKQLCNLNQISSKKLLKPGQKIRVR"
     gene            complement(2550..3413)
                     /locus_tag="PLZ54_06520"
     CDS             complement(2550..3413)
                     /locus_tag="PLZ54_06520"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ66614.1"
                     /translation="MKKLNLFLLVAISATFTFSSCNSDKPGKPTLKSQIDSLNYAFGV
                     ANGDGIKNYYLQGDSTGKALNSLLKGINDGLKSKVDKENMELASIGSQIGYSLKEQSK
                     SGLMGDSSLSVNINLVKQGLINGMKGSNIQMTPQEASEYLQSTMQSLQEKKIEKQYSA
                     NKEAGEKFLLENAKKSGVITTESGLQYEIIKKGKGPIPTDSSKVKVHYHGTLIDGTVF
                     DSSVERNQPAEFYVNQVIKGWTEALKLMPVGSKFKIYVPYQLAYGSANQGAIKPFSML
                     IFEVELLAIEK"
     gene            complement(3463..3990)
                     /locus_tag="PLZ54_06525"
     CDS             complement(3463..3990)
                     /locus_tag="PLZ54_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HOJ66615.1"
                     /translation="MKKQIGFMLATVLAVFLLASCSKKYEAKKVQLKTQNDSLNYTLG
                     LANGEGIKNYYLQNDSSDAPIKALINALDKAYSSKEVDDQMYQLGYQIGTSLKYQKEN
                     GLMGDSTLVFNTKLFKLGLVNGLHGFDGGMTPQQAQEYLQKTMTELQAKKSAQQAPAN
                     QPQPAPESQKTDSVQ"
     gene            complement(4031..4753)
                     /locus_tag="PLZ54_06530"
     CDS             complement(4031..4753)
                     /locus_tag="PLZ54_06530"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ66616.1"
                     /translation="MRKIFVLSLLAIFLITSAEAKKPKKIQEEPPVQKLTNTIDSMSY
                     ALGLSIGSDFIKNLKTIPGGQFNIDWLIQGFTGGMKKDSTVMTTAFANNYFREYITKA
                     QSLESAKKKEVADAFMEKNKTRESVITTESGLQYEILACANGPKPSINDTVEVNYQGF
                     LPDSTKFDSTFDRGEPAVFALNQVIPGWSEGLQLMTVGSKYKFYIPYELAYGEQGAGG
                     VIPPYSPLIFEVELLQINPAKK"
     gene            complement(4851..6632)
                     /locus_tag="PLZ54_06535"
     CDS             complement(4851..6632)
                     /locus_tag="PLZ54_06535"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOJ66617.1"
                     /translation="MKKTCLIFMYLLPVFSFGQVLDDFSDGNFTENPSWSGTTGNFKI
                     NEYFQLQSNAPTTSVSYLFTPSEVFYDAEWQAWFKINYSTSSSNYSAFYIISSSPDNL
                     GNAYFVKIGDTQDDVSLWRQQGTSKIKIIDGTDKRTDGTQVEIRVKVTRDSLGYFSLY
                     SKLPSESDFVLEGTVRDTTIESSSYIGLLYSNTTTTTSAYFFDDIAANGKKAVDKKPP
                     VWEKLAIIEPDTLQLQFSEPVDLSNSIFILNENEITPTKIDILSTKPFLMNVALNENF
                     DKGKIYTLELLQVTDLAGNTLLDNIKKTGIIEPLAQGDLLLNEVMFNAPPTATEYIEI
                     YNASAKTLDVSGIVITTRKSDGSLNTLRHIPDGKLLAAGGYLAVCQHADSVMNYFGLP
                     NDGSIVSISWTALNNQQSTIVLANSKADTVYDELTYNEKWHHLMIKNRQGVALERINP
                     ALPTQSAESWHSAASEVKYGTPGYKNSQYREIENTFSNKQFVWVEPEAFSPDNDGTDD
                     VCFIKYRTPTEGFVANAQIINATGTKVFQLVSNALLSSEGFFSWDGRTDSGKIANAGI
                     YVLYFEMIQPKTGQKKQMKIPIVVSAR"
     gene            6798..7805
                     /locus_tag="PLZ54_06540"
     CDS             6798..7805
                     /locus_tag="PLZ54_06540"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005824925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOJ66618.1"
                     /translation="MRVAIVGASGAVGQEFLRVLAERNFPLTSLSLFGSSRSAGSVYD
                     FKGEKLTVKELKHNDDFKDIDLAFTSAGAGISKEFAEDITKYGALMIDNSSAFRMEND
                     VPLVVPEVNASDAKNRPRGIIANPNCTTIQMVVALKAIENVSHIKRVHVATYQAASGA
                     GAAAMAELELQYRQVLAGETPTVNKFPYQLAFNVIPQIDVFTENGYTKEEMKMFNETR
                     KIMHSDVVVSATCVRVPSLRAHSESIWAETERPLSLEEVKAAFENAEGVVVMDNPAEK
                     LYPMPLFLSGKDPVYVGRIRKDLANPNGITFWCVSDQIKKGAALNAVQIAEYLIKEKE
                     F"
     gene            8087..9523
                     /gene="sufB"
                     /locus_tag="PLZ54_06545"
     CDS             8087..9523
                     /gene="sufB"
                     /locus_tag="PLZ54_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOJ66619.1"
                     /translation="MSEDIINQVTQGEYKYGFTTDIDTEVFPMGLNEEIIRLISVKKN
                     EPEWLLDFRIKSYRHWLTMKMPTWAHLEIPPIDFQKISYFAAPRKKSPKSMDEVDPEI
                     LKTFDKLGIPLEEQKALSGMAVDAVMDSISVKTTFKENLAELGIIFCSFSEAVQHHPD
                     LVQKYLGSVVPYSDNFFATLNSAVFSDGSFVYIPKGVRCPMELSTYFRINASNTGQFE
                     RTLIVADEDSYVSYLEGCTAPMRDENQLHAAIVEIVALKNAEVKYSTVQNWYPGDKEG
                     KGGIYNFVTKRGICKGENSKISWTQVETGSAITWKYPSCILQGDNSSAEFYSVAVTNH
                     FQQADTGTKMIHLGKNTRSHILSKGISAGFGQNSYRGLVRVSKNADNARNFSQCDSLL
                     LGDKCGAHTFPYMDVNNDTAIVEHEATTSKISEDQIFYCNQRGISTEDAVGLIVNGYA
                     REVINKLPMEFAVEAQKLLQITLEGSVG"
     gene            9579..>9971
                     /locus_tag="PLZ54_06550"
     CDS             9579..>9971
                     /locus_tag="PLZ54_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ66620.1"
                     /translation="MLEIKDLHASINGKEILKGVNLTIRAGEIHAIMGPNGAGKSTLA
                     WVLSGNPAVEVTHGEIIFNGKNLLEMSPEDRSREGIFLSFQYPVEIPGVSMVNFMRTA
                     VNEQRKYKNLEPLSAADFLRLMREKKELV"
BASE COUNT         3112 a         1846 c         1941 g         3072 t
ORIGIN      
        1 cgttgattca ggaatctgaa actatggatt taaaaaatct ggaggaaggt taccaacatt
       61 tgagcgaaat ttttccgcaa taccaaatca gtatggtaca cggtaaaatg aaacctgccg
      121 aaaaggaagc cgaaatgcag aaatttgttg ccggaaaaac ccaaataatg gttgctacta
      181 cagtgattga agttggggtg aatgttccca atgcttcggt aatgattatt gaaaatgccg
      241 agcgctttgg tttggctcag ttgcatcaac tcagaggcag agtgggaaga ggagctgacc
      301 agtcgtactg cattttgctg acgggataca aattgagcaa tttgacccgc aaaagaattg
      361 aaactatggt gaacaccaat gatggttttc agatagcaga aaccgatttg caattacggg
      421 gaccgggcga tttggacggg acacagcaaa gcggattacc tttcgagttg aaaattgcca
      481 atcttgcaca ggatggaaaa atgctcgaaa tggcacgaaa agtagctatg gatattatag
      541 aagaagaccc gcagttgcag aaaaaagaaa atttaatact ctctgaacaa ttaaaaaaaa
      601 tgaaaacctc atccttaaat tggagcgtta ttagttaaaa aaaactaata aggttttgtt
      661 tttgattcat ttttttttat ttttgggaaa ccaaaaatct cctgtatata aaacttcctt
      721 tcattacaat ttaaattaat ggaaaaaacg cttgttatta ttaagcccgg tgccattcag
      781 cgcgaactga tcggtgaaat cattacccga tttgaaagga aaggattgca tttgtgcgga
      841 ataaaaatgt atcagtttac cgacaaagtg gtttctgaac attatgctca tttggccgat
      901 cggccttttt ttaagcgtat caaggattca atgatggtta cgcccgtgat tgtttgttgt
      961 tgggaaggac tggacgccgt tagaattgtg cgttccatgt caggtgtaac caatggacgt
     1021 gaagcagctc ccggaacaat aaggggcgat ttcagcatga gcgtgcagga gaatattgtt
     1081 catgcttccg atacggtaga aaatgccaag gtagaaattg accgtttttt caaacctgaa
     1141 gaaatttttg agtttaagcc caataatttt ccttttgttt atgctaatga tgaagtttaa
     1201 tttttttatt aaaagaaaag ttttgatgat ggagtatatt tcgaagtgtt taaaattctt
     1261 ttttttattg cttggttttt tgttgttttg caatgcaata aacgcagaaa aagttaatat
     1321 tcccaactcg acaacttcaa atttaaaaga tactcttaat atttaccacc aaatgttgaa
     1381 tgacacatct gatgatttga tggaaaatca cccggcagac gatatttaca acaacatttg
     1441 gacttccgaa aggttaaatc cctatcattt tccggtagat agtctgcctg attccgtaag
     1501 aattgactgt tccaatttca ttctacctat ttacggaaaa gtaacgtcta aatttggccg
     1561 ccgtcgatat cgttatcatt acggaatcga cttaaaacta aatgtaggcg attcagttcg
     1621 ttgtgccttt gaaggaaagg tacgcattat tgattatgaa catggtggtt atggtcatta
     1681 tgtagtagtt agacacaata acggactgga aacggtttat gctcatcttt caaaagttct
     1741 cgtagaactg aatcaaataa ttggagctgg agaggtaata gctttaggag gaaataccgg
     1801 tcgttccaca ggaccacatt tacattttga aatgcgttat ttggggaacg ccattgatcc
     1861 tgataatttg gttgactttg aaaaaggttc cataagacaa ttttcttacc tgattaccaa
     1921 gaaaaatact ttcaaatatc aaaaagattt gaaaagtctt caaatggcac gctaccatgt
     1981 tgttcataag ggtgatacac ttagtcttat agcttcacgc tatggcacaa cggtaaaaca
     2041 actttgcaat ttaaatcaga tatcatctaa aaagttatta aaacccggtc aaaaaatcag
     2101 agttcgatag gagaggggaa ctatgaaatg ctctcctttt taggtaatga aaaatttttt
     2161 gcttatttca caggttagaa taatagtttt aggctcttca tgtaaaagag aggcataaag
     2221 tagattaaat cctcgcaaca attatttttc cttttttata attcgcaatt acttccttta
     2281 catcattttg catgcaaacc atatctatgc ctgttagcca ttgtttagtt ttggtttgac
     2341 ctgcaagcat cattttcgat tcgattgatg aaaaggataa tcaaaacctc aaaaagtata
     2401 acttactttt cctaaatcaa accaaaacca catgaaaatt ctttttgctg tgctttaaag
     2461 tatttctgta ttatatatgt tcaaaaaaaa aatccctgtt tctaatttta agaaacaagg
     2521 gatgttgtaa gaataaaatt taggaaaaat tacttttcga tcgcaagtaa ttcaacttca
     2581 aaaatcagca tggaaaaagg ttttatggca ccttgattgg cacttccgta agccaattgg
     2641 taaggaacat aaattttaaa tttggaacct accggcatca atttcaatgc ttcagtccaa
     2701 cctttaatca cctgattgac ataaaattcg gcaggttgat ttctttcaac agagctgtcg
     2761 aaaacagttc catcaatcaa cgtcccatga taatgaactt tgactttgct gctgtcggtt
     2821 gggataggac ctttgccttt tttgatgatt tcgtattgta aaccactttc tgtagttatt
     2881 actcccgatt tttttgcatt ttcaagcaaa aacttttcac cggcttcctt gttggcacta
     2941 tattgttttt ctattttctt ttcctgaaga ctttgcatgg tagattgcaa atattcgctg
     3001 gcttcctgag gagtcatttg gatgttcgag cctttcatgc cattgattaa accttgttta
     3061 accaaattga tattgacaga taaagaagag tcgcccatta aaccactttt gctctgttct
     3121 ttaagagagt atccaatttg agaaccaata ctggcaagtt ccatgttttc tttgtccact
     3181 ttggatttta aaccatcgtt gattcctttt aacaaagaat ttaaagcttt gccggtagaa
     3241 tcgccctgta aataataatt tttgattcca tcaccattgg caacgccaaa agcataattc
     3301 aaactgtcga tttgcgattt aagagtcggc ttgccgggtt tatcgctgtt acatgaagaa
     3361 aaagtgaaag tagctgaaat ggctaccaga agaaataaat ttaatttttt catcttacaa
     3421 aaattatatg agattttatt gtaatgtctt aattcatttg aattactgca cactgtcagt
     3481 tttttgactt tctggagccg gttgaggctg attagcaggt gcttgttgag ccgatttttt
     3541 tgcttgtaac tcagtcattg ttttttgtaa atactcctga gcttgttgag gtgtcattcc
     3601 accatcgaaa ccgtgaagtc cgttgacaag accgagttta aacaacttgg tattgaaaac
     3661 caacgtggaa tcacccatca acccattttc tttctgatac ttcagagaag ttccaatctg
     3721 ataacccaac tgatacatct gatcatctac ttctttactg gaataagctt tgtccaaagc
     3781 atttatcaaa gctttaatgg gagcatccga tgagtcgttt tgtaaatagt aattcttaat
     3841 tccttctcca ttggcaagac ccaacgtgta attgagactg tcattttgag tttttaattg
     3901 gacttttttt gcttcgtatt ttttactaca agaagccaac aaaaaaacgg caagaactgt
     3961 tgccaacata aaaccgattt gttttttcat tttgactgac ttattttttt atgatacaaa
     4021 aatttttagt ttatttttta gctggattta tttgcaataa ttccacttcg aaaatcagtg
     4081 gagaataagg aggaataacg cctccggcac cctgctcgcc gtaagcaagc tcatatggaa
     4141 tataaaattt atatttggaa ccgacggtca tcagttgcag gccttcactc catcccggaa
     4201 ttacctgatt gagagcaaaa actgcaggtt ctcctctgtc aaaagtactg tcgaacttgg
     4261 tactatcggg aaggaaacct tgataattaa cctcaaccgt atcattgata gatggttttg
     4321 gaccattggc acatgcaagg atttcgtact gcaaaccact ttcagttgtt attacacttt
     4381 ctctggtttt gtttttttcc ataaaagcat cagcaacttc ttttttcttt gccgactcta
     4441 aggattgagc tttagtaatg tattcacgaa aataattgtt ggcaaaagca gtggtcatta
     4501 cagtagaatc ttttttcata ccgccggtaa atccctgtat caaccaatca atattaaatt
     4561 gaccacccgg aatggttttc aaatttttaa taaaatcaga tccaatgctc aaacccaaag
     4621 cataactcat ggaatcgata gtgttggtga gtttctgaac cggaggttct tcttgaattt
     4681 ttttgggctt tttggcctct gccgaagtta tcaagaaaat tgccaataaa gataaaacaa
     4741 atatttttct catttttata gtgaataatt gatttttaga atattaaaac tttaaaaatt
     4801 tcagcaaaga taactgattt attgcattcc aacatgttaa tttttcaaaa ttatcgtgca
     4861 gagaccacaa ttggaatttt catctgcttt ttttggccgg tttttggttg aatcatctca
     4921 aaatagagta cataaattcc tgcattagca attttcccgc tatcggtgcg gccatcccaa
     4981 gaaaagaaac cttccgagga tagcaacgca ttgctaacca actgaaaaac ttttgtcccg
     5041 gtcgcattaa taatttgagc gttagccaca aaaccttctg tcggagtacg atattttatg
     5101 aaacagacat catccgtacc atcgttatcg ggagaaaaag cttcgggttc aacccaaacg
     5161 aattgtttgt ttgaaaaagt gttttcgatt tcacgatatt gtgaattttt atagccgggt
     5221 gttccgtatt taacttcaga ggctgccgag tgccaacttt ccgccgattg tgtgggcaga
     5281 gccggattaa ttcgctccaa tgccacaccc tgccggtttt ttatcattag gtgatgccat
     5341 ttttcattgt acgtcaattc atcatataca gtatcggctt tggaattggc aagcacaatg
     5401 gtactttgct gattgttgag tgccgtccac gaaatggaaa cgatactgcc atcgtttggc
     5461 aggccaaaat aattcattac cgaatcggca tgctgacata cagccaaata gcctccggct
     5521 gccaacaatt taccgtcagg aatatggcgc aaagtattca atgaaccatc cgatttgcgt
     5581 gtagttatga ctattcccga aacatccagc gttttggctg aagcattgta gatttcgatg
     5641 tattcagtag cagtaggagg tgcattaaac attacttcgt tcagcaataa atctccctgc
     5701 gctaaaggtt caataatacc ggtttttttg atattgtcaa gtaacgtatt tcctgccaaa
     5761 tcggttactt gcaaaagttc aagcgtataa atttttccct tatcaaaatt ttcgttcagc
     5821 gcaacattca tcaaaaaagg tttggttgat aaaatatcga tttttgtagg agtaatttca
     5881 ttttcgttca aaatgaaaat tgaattggac aagtcaaccg gttcagaaaa ttgcaactgc
     5941 aaggtatcag gttcaattat ggcaagtttt tcccaaacag gtggtttttt atccacagct
     6001 tttttcccgt tagcagcaat atcatcgaaa aaataagctg aggttgtagt cgttgtattg
     6061 gaataaagca aaccaatata tgagctgctt tcgatagttg tatcgcgaac cgttccttcc
     6121 agcacaaaat cactttcgga agggagttta ctgtaaagag aaaagtaacc cagtgaatcg
     6181 cgggtaactt ttacccgaat ttcaacctgt gtaccatctg tgcgcttatc tgtgccatca
     6241 atgattttga tttttgacgt tccttgctgt ctccacaacg aaacatcgtc ctgcgtatca
     6301 ccaattttta caaaataagc atttcctaaa ttatcgggag aactcgaaat aatgtaaaaa
     6361 gcggaataat tggaagaaga agtggaataa ttaattttaa accatgcttg ccattcggca
     6421 tcgtaaaaaa cttccgatgg cgtaaacaaa taggaaacgg aagttgttgg agcattggat
     6481 tgcaactgaa aatattcgtt tatcttaaaa tttcctgtcg ttccgctcca tgatggattt
     6541 tcagtaaaat tcccatcaga aaagtcgtca agtacttgtc cgaaagaaaa gacaggcagt
     6601 aaatacataa aaataagaca ggttttcttc attttttatg aaaattctat gatttagatt
     6661 aattttgtac cgaaaaaaga agaagaatca attttttaag acaatatact gcaaaaataa
     6721 aaatttctta acaaagaaaa ttatttgcgt gcatatttct ttttaccaaa catacaaata
     6781 accaaaattt tacaacaatg agagtagcaa ttgtaggcgc cagcggagct gttggccagg
     6841 aatttttgcg cgttttagcc gaacgcaatt tcccattgac ttccttgagc ctgtttggct
     6901 cttcccgtag tgcgggcagc gtatatgatt ttaaaggaga aaaacttacc gtaaaggaat
     6961 taaaacataa cgacgatttt aaagacatag acttggcatt tacctctgca ggagccggaa
     7021 tatccaaaga atttgccgaa gatatcacca aatacggggc attgatgatc gacaattcat
     7081 ctgcttttcg gatggaaaat gacgtgccgt tggtagttcc tgaagtaaat gcttccgatg
     7141 caaaaaatcg tccccgcggc atcattgcca atcccaactg cactaccatt caaatggttg
     7201 tagctttaaa agccattgaa aacgtttcac acatcaaacg cgttcatgtt gccacgtatc
     7261 aggctgcaag tggtgcaggt gccgctgcaa tggccgaatt ggaactgcag taccgtcagg
     7321 tattggctgg tgaaacaccg acagtgaata aatttcctta tcagctggct ttcaatgtaa
     7381 ttcctcaaat tgatgtattt actgaaaacg gctatacaaa agaagaaatg aaaatgttca
     7441 acgaaaccag aaaaatcatg cattccgacg tagtggtgag tgctacctgt gtgcgcgtgc
     7501 cttcattgcg tgcgcattcc gaaagcatct gggccgaaac tgagcgtcct ctgagcttgg
     7561 aagaagtgaa agctgctttc gaaaatgccg aaggtgtggt ggtgatggat aatccggctg
     7621 aaaaattata tcccatgcca cttttcctgt cgggtaaaga tcctgtatat gtaggacgta
     7681 tccgcaagga tttggcaaat cccaacggaa taacattctg gtgcgtttcg gatcagataa
     7741 aaaaaggagc agcactgaat gcagttcaaa tagctgaata tctgatcaag gaaaaagagt
     7801 tttgatgaag gagaactttt ttcttctaca attttaacaa aaaccgtcat aacatgttgg
     7861 tatggcggtt tttgttttca acttattttg tttttgagat taaaaatcaa agtttttcat
     7921 gaagttgcga taaaagggag gtcaattaat tctacaaaaa agaaatttac caaaacaaaa
     7981 tctgagtttt aaatgttatc aaaaatacaa tgaaatttta gaatttctga tacaatttct
     8041 aaaatttaca agcaactttt ttgacaagta aaaaattttt caatttatgt cagaagatat
     8101 aataaatcaa gttacacagg gagaatacaa gtacggtttc actaccgaca tcgatacaga
     8161 agtttttccg atgggactta acgaagaaat tattcgactt atttcggtaa agaaaaacga
     8221 accggaatgg ttgctcgatt ttcgtatcaa atcctatcgc cattggctga caatgaaaat
     8281 gccgacttgg gctcatttgg aaattccgcc cattgatttt cagaaaatat cctattttgc
     8341 ggcgccacga aaaaaatcgc caaaaagtat ggacgaagtt gatcctgaga ttttgaaaac
     8401 cttcgacaag ttgggaattc cgctcgaaga acagaaagcg ctttcaggta tggctgttga
     8461 tgccgtgatg gacagtattt cggtaaaaac aacgttcaag gaaaatctgg cagaattggg
     8521 tattattttc tgttcattca gcgaagccgt gcaacatcat cccgatttgg ttcaaaaata
     8581 tctgggttca gttgttcctt attccgacaa tttttttgca acgctcaata gtgccgtttt
     8641 cagcgatggt tcttttgtgt acattcccaa aggtgttcgc tgtccaatgg aactttccac
     8701 ctactttcgc atcaacgctt ccaataccgg tcagtttgaa agaacactga tcgttgccga
     8761 cgaggacagt tatgtttcat atcttgaagg ttgtacggct ccaatgcgcg acgaaaatca
     8821 gcttcatgct gccattgtgg aaatcgtagc actcaaaaat gccgaagtaa aatattctac
     8881 cgtacaaaac tggtatccgg gagacaaaga agggaaaggc ggaatttaca attttgtaac
     8941 caaacgcggc atttgcaagg gagaaaattc caaaatttcg tggacacaag ttgaaaccgg
     9001 ctcggccatt acttggaaat atcccagttg cattttacag ggcgacaatt cgtcggccga
     9061 attttattcg gtagcggtta ccaatcattt tcagcaagcc gataccggca ccaaaatgat
     9121 tcatttggga aaaaacaccc gcagccacat tctctcaaaa ggaatatctg caggtttcgg
     9181 ccagaacagc tatcgcgggt tggtgcgagt ttcaaaaaac gccgacaatg ctcgaaattt
     9241 ttcgcaatgc gacagcctcc tgctgggcga taaatgtggt gctcatactt ttccatacat
     9301 ggacgtcaac aacgatacag ccatagtgga acacgaagcc accacttcca aaatcagtga
     9361 agaccagatt ttttactgca accaaagagg aatttccact gaagatgctg ttggcttgat
     9421 tgtaaatggt tacgcaaggg aggttatcaa taaattgcct atggaatttg ccgtagaagc
     9481 acaaaaatta ttacagataa cgttggaagg ttctgtggga taaaacctgt taaccttttg
     9541 aaagaaattt ttattacaga ttattagaaa aatacattat gttggaaata aaagatttac
     9601 atgcatccat caacggaaaa gaaattttaa agggagtaaa cttaacaatt cgtgccggtg
     9661 aaatacatgc cattatgggc ccgaatgggg ctggaaaatc cactttggcg tgggttttgt
     9721 ccggcaatcc ggctgtggag gtaacacacg gcgaaattat ttttaacggc aaaaatttgc
     9781 tcgaaatgtc gccggaagat cgttcgcgcg aaggaatttt cctgagtttt caatatccgg
     9841 tcgaaattcc aggtgtcagc atggtcaact tcatgcgtac cgccgtcaat gaacaacgta
     9901 aatacaaaaa tctcgaacct ctttcggctg ccgattttct gcgcttgatg cgcgaaaaga
     9961 aagaattggt c
//
LOCUS       DAOSCH010000077         9890 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4485_length_9890_cov_6.052771, whole genome shotgun sequence.
ACCESSION   DAOSCH010000077 DAOSCH010000000
VERSION     DAOSCH010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9890)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9890)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9890
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4485_length_9890_cov_6.052771"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..232)
                     /locus_tag="PLZ54_06555"
     CDS             complement(80..232)
                     /locus_tag="PLZ54_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOJ66621.1"
                     /translation="MAKKVVASVQKGEGRTFSKVIKMVKSPKTGAYMFTEEMVPNDKV
                     KDFLNK"
     gene            complement(237..425)
                     /gene="rpmG"
                     /locus_tag="PLZ54_06560"
     CDS             complement(237..425)
                     /gene="rpmG"
                     /locus_tag="PLZ54_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOJ66622.1"
                     /translation="MAKKTKEARVQVILECTEHKDSGMPGTSRYVTQKNRKNTPERLE
                     LRKYNPILKRVTIHREIK"
     gene            complement(448..684)
                     /gene="rpmB"
                     /locus_tag="PLZ54_06565"
     CDS             complement(448..684)
                     /gene="rpmB"
                     /locus_tag="PLZ54_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOJ66623.1"
                     /translation="MPKICQITGKKPLKGNNVSHSKRRTKRNFDVNLFTKKFYWVEED
                     TWISLDISAAGLRTINKLGLDASIKKALKKGYLK"
     gene            complement(1101..1973)
                     /gene="rfbA"
                     /locus_tag="PLZ54_06570"
     CDS             complement(1101..1973)
                     /gene="rfbA"
                     /locus_tag="PLZ54_06570"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOJ66624.1"
                     /translation="MKGIVLAGGSGTRLYPITKSISKQIIPVYDKPMIYYPLSILMLA
                     GIREILIISTPQDIHLYERLLGDGSDFGIAIEYAVQPSPDGLAQAFIIGENFIGNDNV
                     CMILGDNIFYGFDFSRTLIEAANLKDGAIVFGYYVNDPERYGVAEFDAEGKVLSLEEK
                     PKQPKSNYAVTGLYFYSNDVVEKSKHLKPSSRGELEITDLNRLYLNEGRLNMKILGRG
                     MAWLDTGTHDSLLDASNFIATIENRQGLKIACLEEIAYRNGYIDREQLLRLAEPLKKS
                     HYGEYLIKIAGEVK"
     gene            complement(2052..2597)
                     /locus_tag="PLZ54_06575"
     CDS             complement(2052..2597)
                     /locus_tag="PLZ54_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4924 family protein"
                     /protein_id="HOJ66625.1"
                     /translation="MFIAQRLKKENICEYLLYMWQIEDLIRAFQLNIDLIDENIIAPY
                     PISETEKRTMYEWYESLIDMMRKENVQEKGHLQLNKNVIIDLNDFHELLLQSGADSEY
                     NRLFYSILPHLVQLRKKAEVNINDIELCFNFLYGIMMLNLKKEKISDETAAAQNDISK
                     FMALLAKDYKLYQEGELDLYK"
     gene            3001..3858
                     /locus_tag="PLZ54_06580"
     CDS             3001..3858
                     /locus_tag="PLZ54_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOJ66626.1"
                     /translation="MKKLGILFFAIILLICSGFVSAQQKKTITLNVATFNLRLDTEDD
                     GENAWPNRKEFVKSLIRFHDFDIFGTQEGYKHQLDDILKLGGYAYVGVGRDDGVDEGE
                     HSAIFFKTNRFEVLDKGNFWYSKTPEVPSKGWDAFCCNRLCSWGKFKDKSSGKIFFVF
                     NSHFDHIGHIARKNSSLLLLKRIGEIAGNFPVFCTGDFNSTPDAEPIQIIAKSGKLFD
                     SYHITKQPPYGTVGTYNSFSIKSKMDDRIDYIWVTSGISINKYGVLNEQQYGRFASDH
                     FPVMVSATL"
     gene            3983..6004
                     /gene="ligA"
                     /locus_tag="PLZ54_06585"
     CDS             3983..6004
                     /gene="ligA"
                     /locus_tag="PLZ54_06585"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOJ66627.1"
                     /translation="MTVKEQIEALRQEIEQHNYYYYVLSQPIISDYEYDMKMKQLQEL
                     EEKYPEYFDPLSPTQRVGSDITTSFEQAYHTYPMLSLGNTYTEGEVKDFYERVRKALN
                     EPFELVCELKYDGVSISITYEHGEIQRAVTRGDGEKGDVVTANIKTIRSIPLRLKGYY
                     PDKFEIRGEVLMPWAVFEALNKEREEQEEPLFANPRNAAAGTIKLQNSAIVASRKLDA
                     YFYYVLGEQLPFDTHFENLQAARGWGFKISENIKLCRTLDEVWEFIHFWDKERKNLPV
                     PIDGIVLKVNSLEQQRKLGTTAKSPRWAIAYKYQAEKALTKLLSVTFQVGRTGTVTPV
                     ANLEPVQLSGTTVKRASLHNADIIESLDLHIGDMVYVEKGGEIIPKITGVEKSARPQN
                     GEKVVFIKNCPECNAPLIRYPGEAAHYCPNENGCPPQIKGKIEHFVSRKAMNIEGLGE
                     ETINMLYENGLLHNVADIYDLKISDLVSLERLGLKSASNLISSIEKSKEVPFERVLFA
                     LGIRFVGETVAKKLAYAFKSIEALQQADYEDLIQVEEIGDKIAQSVRQYFSDPKNLEI
                     INRLKAAGVQMSLNDDKSKRKGNELEGKSVVISGTFKYHSREEYKDLIEAHGGKNVGS
                     VSSKTSFILAGENMGPEKLKKAQNLGIELIDENKFLSMINEEIPSSATE"
     gene            6087..6656
                     /locus_tag="PLZ54_06590"
     CDS             6087..6656
                     /locus_tag="PLZ54_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66628.1"
                     /translation="MSRKKIKNAAIKLSDANFHIPKFLNYSDIHSILLLFEADDAEKN
                     LQVKKIVQMLKADGKKVTAIGCPRNKKLKTDFPIFENLTIVDKSQTDFFEKPRHSVFK
                     PIEANKFDALLDLSLNENIPLQYIALYSNALLKIGVRKTSFPLYDFILDIDSIVANLK
                     TDHLPIDLLYIFNQIIFYLKNIQSCDEKA"
     gene            6687..7574
                     /gene="dapA"
                     /locus_tag="PLZ54_06595"
     CDS             6687..7574
                     /gene="dapA"
                     /locus_tag="PLZ54_06595"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOJ66629.1"
                     /translation="MTINNKLTGMGVALVTPFKSDDSIDFDALGRLVEYIINNNADYL
                     VVCGTTAETPTLTDKEKDEIARFVVQCNAGRLPIVLGIGGNNTRQVVEKVKTSDLSGI
                     DAILSVTPYYNKPSQEGLYQHYSAIAKASPLPLVLYNVPGRTGVNMTAETTLRLAKEF
                     SNICAVKEASGNFTQIDDIIKNKPQDFMVISGDDGITFPLITLGAVGVISVIGNAFPR
                     EFSRMVRLALQGDYQNARQIHYRFTELFELLFVEGNPAGVKSMLAVMGMIENKLRLPL
                     VPNTIKTYEKIRSVLNQLN"
     gene            7700..8689
                     /locus_tag="PLZ54_06600"
     CDS             7700..8689
                     /locus_tag="PLZ54_06600"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HOJ66630.1"
                     /translation="MAIDTKYLTSDTIGSVVQDLFNPSNVGAKPFLKWAGGKGQLLDI
                     FQKLYPEQLKRKKIKNFYEPFLGSGAVFFDITQKYEIENAFLYDINEELILAYKVIQR
                     DVSKLIDFLYRYQKTYLKLDKVKRKEFFYDQRTNYNLQRFNIDYDKYSENWVPRAAQL
                     IFLNRTCFNGLYRVNLKGEFNSPVGDYDNPTICDEQNLLAVNKVLEIAEIKKADFKDI
                     VTDLRSDSFVYFDPPYRPISKTASFKAYHKQGFTDSEQLQLAQLFKQLDSEGSYVMLS
                     NSDPKNIDPDDNFFDDMYNGFNIVRVPARRMINSDPTKRGRINEIVVTNYTTA"
     gene            8689..9180
                     /locus_tag="PLZ54_06605"
     CDS             8689..9180
                     /locus_tag="PLZ54_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66631.1"
                     /translation="MEKGTKSYISGNQLEVAVKTVLIGKGFELVKYRDWEKNQEKYGK
                     ELLLENVPFTTIYEHNGNTEFLLISEKYELKIRIECKWQQVGGSVDEKLPYLYLNTIE
                     AMPEETIMILIDGSGWKTGAIKWLKDAVKQKKYTSEENKHKEILVFSLTDFFTWANKT
                     FNR"
     gene            complement(9405..9737)
                     /locus_tag="PLZ54_06610"
     CDS             complement(9405..9737)
                     /locus_tag="PLZ54_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66632.1"
                     /translation="MDNSLEEKVIDEVSETTNSDEIEEVNLSAEKTLKVIATIILIGG
                     IIGTLICLFTIVMVQNPEYTYTKDLIFNPMGFVTTVSVLLGSLTIWASLRVLSDISMN
                     LKELNSKK"
BASE COUNT         3197 a         1712 c         1775 g         3206 t
ORIGIN      
        1 cttaaacggg ctgcttaata taaaaaagaa agtgtccttt tgggacaccc tctttgtagg
       61 taggaaataa tttatttttt catttattga gaaaatcttt tactttatca ttgggcacca
      121 tttcttcagt aaacatgtaa gcccctgttt taggtgattt gaccattttt atcacttttg
      181 aaaaagtacg accttcacct ttttgtactg atgcaacaac cttctttgcc ataatcttat
      241 tttatttctc tgtgaatagt aactcttttt aatattggat tgtatttcct gagctcaagc
      301 ctttcgggag tattttttct gttcttttga gtaacatagc gcgaagttcc gggcatacca
      361 ctgtccttat gttcggtaca ctccagaata acttgcaccc ttgcttcttt cgtttttttt
      421 gccatgatgt atgcctccta atagtattta ttttaaatat ccttttttca gcgctttttt
      481 aatggaagcg tccaatccca acttgttgat ggttcttaaa ccggcagccg aaatatcaag
      541 actaatccat gtgtcctctt ccacccaata aaactttttg gtaaataagt ttacatcgaa
      601 atttcttttt gtccgtcttt tcgagtgtga aacattattt ccttttaaag gttttttacc
      661 tgtaatctga caaatcttag gcattttatt tcatttatgt tgttatactt ctgctcaaaa
      721 ttgagttgca aaattacatt ttatttttta catatacaag caattgtatc cctatttttt
      781 gaaatttatc tttataaact cattaaaatg tttgttaatc aatttgttaa ttgctttttc
      841 tttacagaaa aatccatttt taatcgggtc tgaaaaattt acataaacca tagtaaatca
      901 ataaaatatc tttcaggaaa gcgacaggtt tatgcaagtt tcagttatgt ataaaaccag
      961 taaacaaatt atattttcaa aggataaaaa cattcaagaa aagtgtatca tttaagtttt
     1021 gaaaatatat ttaaaagcgt cttatgcgta ataaaagaga ctctctcact tttgtagcca
     1081 aaaatagttt tagaatcaaa ctatttgact tcacctgcaa ttttaatcaa atattcgcca
     1141 taatgacttt ttttcaatgg ttcggccagt cgaagcagtt gttcacggtc gatatacccg
     1201 ttgcgatagg caatttcttc gaggcaggct attttcagcc cctgacggtt ttctatggtg
     1261 gcaataaagt tggaagcatc cagcaaactg tcatgtgttc ctgtatcgag ccaggccatt
     1321 cctctaccca aaattttcat attcaatcgt ccttcgttca ggtaaagtct gtttaaatcg
     1381 gtaatttcca attctccacg cgatgaaggt ttcaggtgtt tcgatttttc aactacatca
     1441 ttactataga aataaagtcc tgtaacggca taatttgatt ttggttgttt tggtttttct
     1501 tccaaactca ataccttgcc ttcagcatca aattcggcta cgccatatcg ttccggatca
     1561 ttgacataat aaccgaaaac aatggctccg tctttcaggt tggccgcttc gatcaatgtt
     1621 ctggaaaagt cgaatccata aaaaatattg tcgccaagaa tcatgcaaac attgtcgttt
     1681 ccaatgaaat tttcaccaat aataaaggcc tgagccaacc catccggcga tggctgcact
     1741 gcatattcaa ttgcaatacc gaaatcagaa ccatcaccca acagacgttc gtaaagatga
     1801 atatcctgcg gtgtggaaat aatgagaatt tcacgaatgc ccgccaacat aagtattgag
     1861 agcggataat aaatcatggg tttgtcgtaa accggaataa tttgtttgga aatgcttttg
     1921 gttatgggat agagtcgagt accggaacca cctgccaaaa caatgccttt catgatgaag
     1981 atttttttaa attgtttttt ctgcgaaatt attttaaacg ataaaaatgt aagcgaattc
     2041 tcaaaagttt tttatttgta caaatccagt tctccttcct gataaagctt ataatctttt
     2101 gccagcaatg ccatgaactt tgaaatatcg ttttgcgcag cggccgtttc atctgaaatt
     2161 ttttcctttt tcagattcaa catcatgata ccatacaaaa aattaaaaca taactctatg
     2221 tcgtttatgt ttacttcagc tttttttctc aactgaacaa gatgaggtaa aatactgtaa
     2281 aataaacgat tgtattccga atcggcgccc gattgcaaca aaagttcatg gaaatcgttc
     2341 aggtcaataa ttacattttt attcaattgc aaatgacctt tttcctgaac gttttctttg
     2401 cgcatcatgt cgatcagact ttcataccat tcgtacatgg ttcttttttc agtttcagaa
     2461 atcgggtacg gagcaattat attttcatct attaagtcga tgttcagctg aaaagcccga
     2521 atcaggtctt cgatttgcca catgtaaagc aagtattcgc agatattttc ctttttcaat
     2581 ctttgagcta tgaacatgat gaaagatttt gatggtacga atataatgcc aatattttta
     2641 agaataggaa tttgtgtctc ttactgaact attttgcaaa attaatataa atatcgggaa
     2701 ttttgcccaa attttttgta gtgaattctg attaaaaata tttgttcaac ccttctttta
     2761 ttttattttt taatctttct ttaaagcatt atgcaggtaa ttcgaaataa ttagagaaaa
     2821 aaagcatagt aaaaaccgaa attcaaaaaa atattagtaa tcttgtattt cattacagat
     2881 agtaaaaatt ttttgtcggc caatttattt tcatgcatta actgttatga aatcaataag
     2941 ttgggttgac tgaaaaaatt taattcgacc atatatcttc tttaacaaca aatacacaaa
     3001 atgaaaaaac ttggcattct gttttttgca attattcttt taatttgctc cggctttgta
     3061 tcggcacagc agaaaaaaac cattacgctc aacgttgcaa cttttaatct tcggctcgat
     3121 acggaagatg atggtgaaaa cgcatggccc aatcggaaag aatttgtcaa aagtctgatt
     3181 cgtttccacg acttcgacat ttttggaaca caggaaggtt ataaacatca attggacgac
     3241 attttaaaat tgggtggata tgcttatgtt ggcgtaggac gagacgatgg cgttgatgaa
     3301 ggagaacatt ctgctatttt tttcaaaaca aatcgctttg aagtattgga taagggaaac
     3361 ttttggtatt ccaaaacgcc tgaagttcct tcaaaaggtt gggatgcctt ctgttgcaat
     3421 cgtctttgtt cgtggggaaa atttaaagac aaatcttcag gaaaaatatt ttttgttttt
     3481 aattctcatt tcgatcacat tgggcacata gcacgaaaaa attcatcctt actgcttttg
     3541 aagcgtattg gtgaaatagc cggaaacttt ccggtttttt gtacgggcga ttttaattct
     3601 acacccgatg ccgagccgat tcaaattatt gctaaaagcg gaaaactttt cgacagttat
     3661 catattacca aacaacctcc ttatggaacg gtgggtacat ataattcatt ttcaataaaa
     3721 tccaaaatgg acgatcgaat tgattatatt tgggttacga gcggtatttc catcaataaa
     3781 tatggggtac ttaacgaaca gcaatatggg cgctttgctt ccgatcattt cccggtaatg
     3841 gtcagcgcta ctctttgaaa ttttaatttt atttttttga gggcgggaat tttgcgactt
     3901 tcttccaatt tccgcccttt tcttgttcac ttttctttaa ttgattaatt ttgcccatca
     3961 ggaattatta tttttctaat ttatgacagt caaggaacaa atcgaagcgc tgcgccagga
     4021 aatagagcag cacaactatt attattacgt gctttcgcaa cccattattt ccgattacga
     4081 atatgatatg aaaatgaagc agttgcagga attggaagaa aaatatcctg aatattttga
     4141 tcctctttcg cccacccagc gtgttggcag cgatattaca accagttttg agcaggctta
     4201 tcatacctat cccatgcttt cgttgggaaa tacttataca gaaggtgaag tgaaagattt
     4261 ttatgaacgt gttcggaaag cattaaatga accttttgaa ctggtatgtg aactgaaata
     4321 tgatggggtt tccatttcca ttacatacga acatggtgaa attcaacggg ctgtaacgcg
     4381 tggagacgga gaaaaaggtg atgtagtaac agccaacatc aaaactatcc ggagcattcc
     4441 cttgcgattg aaaggctatt atcccgataa atttgaaatt cgcggcgaag tgttgatgcc
     4501 gtgggcagtt tttgaagcac tcaataagga aagagaagaa caggaagaac cgctttttgc
     4561 caatcctcgc aacgctgctg cgggaaccat taaacttcaa aattctgcca tcgttgcttc
     4621 ccgtaagctg gatgcctatt tttattatgt attgggcgaa caacttcctt ttgacacgca
     4681 ctttgaaaat ttgcaggcag ctcgtggttg gggttttaaa atttctgaaa atattaaact
     4741 ttgcagaaca ctcgacgaag tgtgggaatt tattcatttt tgggacaaag aacgaaaaaa
     4801 tttaccggtt ccgattgatg gcattgtatt gaaagtcaat tcattggaac aacaacgaaa
     4861 acttggcaca actgcaaaat cgccacgttg ggccattgct tataaatacc aggccgaaaa
     4921 agcattgacc aaacttcttt cggttacgtt tcaggttgga agaacaggca ccgtaacacc
     4981 cgtagccaat cttgaaccgg ttcaactttc gggaactaca gtgaaacgcg cttcgttgca
     5041 taatgcagat attattgaat cgctcgattt gcatattggc gatatggttt atgtcgaaaa
     5101 aggtggagaa attattccga aaattaccgg tgtggaaaaa agcgcacggc cacaaaacgg
     5161 cgaaaaagtt gtttttataa aaaattgtcc cgaatgcaat gctcctctca ttcgatatcc
     5221 cggcgaagct gcccactatt gtcccaacga aaacggttgc ccgcctcaga ttaagggaaa
     5281 aattgaacat tttgtgagca gaaaagccat gaacatcgaa ggtttaggtg aagaaactat
     5341 taatatgctt tacgaaaacg gtttgcttca taatgtggcc gatatttacg atttgaaaat
     5401 ttccgatttg gtttcattgg aacgattggg tttgaaatcg gcttctaatc tgatcagttc
     5461 aatagaaaaa tcgaaggaag ttcctttcga acgcgtattg tttgcgctgg gaattcgttt
     5521 tgttggcgaa acggttgcca aaaaactggc ttatgccttt aaatcaattg aagctttgca
     5581 acaagccgat tatgaagatt tgattcaggt ggaggaaatt ggagataaaa tagctcaaag
     5641 tgtccgacaa tatttttctg atccgaaaaa tctggaaatt attaaccgct taaaagctgc
     5701 cggagttcaa atgtctctta atgatgataa atccaaacgc aagggaaacg agctcgaagg
     5761 aaaatcagtc gtcatcagcg gaacattcaa ataccattca cgtgaggaat acaaagattt
     5821 aatcgaggca catggcggaa aaaatgttgg cagcgtgtct tcaaaaactt cttttattct
     5881 ggcaggtgaa aacatgggac cggaaaaact gaaaaaggca caaaatttgg gcattgaact
     5941 aattgatgaa aataaatttc tttcgatgat aaatgaagaa attccatcat cagccactga
     6001 atgaataaaa ttataaaaca gttcaatttc tgtgtttttt caggacaacc caaaacaacc
     6061 tatttaaaga gattacaatg aaatttttga gcagaaaaaa aattaaaaat gcagctatca
     6121 aattgtccga tgccaatttt catataccca aatttctaaa ttattcagac attcattcta
     6181 ttttgctgtt gtttgaagcc gatgatgcgg aaaaaaatct tcaggtgaaa aaaatagtgc
     6241 agatgctgaa agcagatggg aagaaagtta cagctattgg ttgtcctcga aataagaaat
     6301 tgaaaaccga tttcccgatt tttgaaaatt taacgattgt ggataaaagt cagaccgatt
     6361 tttttgaaaa acccagacat tctgtcttta aaccaatcga agccaacaaa tttgatgctt
     6421 tgctggattt atccttgaac gaaaatattc ctcttcaata tattgctttg tattccaatg
     6481 cattgttgaa aattggagtt agaaaaacat cgtttccatt gtatgatttt atattggata
     6541 tcgacagcat tgttgcaaac ttaaagactg accatttgcc catcgatttg ttatatattt
     6601 ttaatcagat aattttttac ctgaaaaata tacaaagttg cgatgaaaaa gcgtaatttt
     6661 gtaacagaat aaaatttttt cactccatga cgattaacaa caagctcaca gggatgggtg
     6721 tagctttagt aacacctttt aaaagcgacg attcgataga ttttgatgcg ttgggaaggt
     6781 tggttgaata cataatcaac aacaatgccg attacctggt tgtatgtggc actacagccg
     6841 aaacacctac cttgacagac aaggaaaagg atgaaatagc ccgttttgtt gttcagtgca
     6901 atgccggaag attgcctatt gtattgggaa ttggcggaaa caataccaga caagtggtcg
     6961 aaaaagtgaa aacttccgat ttatcgggga ttgatgccat tttatcggtt acgccgtatt
     7021 acaacaaacc ttctcaggaa ggactttatc aacattattc ggccatagcc aaagcttctc
     7081 cgttgccact tgtgctctac aatgtgccgg gaagaacagg cgtgaacatg actgctgaaa
     7141 ctactttacg cttggcaaaa gaattttcca atatttgcgc agtgaaggaa gcctccggaa
     7201 attttaccca gatcgacgat attatcaaaa acaagcctca ggatttcatg gttatttccg
     7261 gcgacgacgg cattactttt ccgttgatta cgttgggtgc tgtcggtgtg atttccgtta
     7321 tcggcaatgc ttttccgcgc gaattcagcc gtatggttcg tttggctctg cagggcgatt
     7381 atcaaaatgc acgtcagata cattatcgtt tcaccgaact ttttgaattg ctttttgtgg
     7441 aaggaaatcc tgccggcgta aaaagcatgt tggctgtcat gggaatgata gaaaacaaat
     7501 tgcgtcttcc tctggtgccc aataccatta aaacgtacga aaaaattcgc tcggtactca
     7561 atcagttaaa ttgaaaaatt acaaaattac aaaaaatttt ccccgacagg acgaaagttg
     7621 tcatgctaaa aaatgctaca tcattagaat gaagtaacac aaaaaatatt tttgtaaatt
     7681 tggacattca aatagaatta tggcaattga tacaaaatat ttaacttctg acacaattgg
     7741 ttctgttgtg caagatttat ttaacccatc taatgttggc gcaaaaccat ttttaaagtg
     7801 ggctggtggc aaaggacaac ttttggatat ttttcagaaa ctttatcctg aacaacttaa
     7861 gagaaaaaag atcaagaatt tttatgagcc atttttagga agtggagcag ttttttttga
     7921 cataactcaa aaatatgaaa ttgaaaatgc ttttctttat gatattaatg aagaactaat
     7981 tctggcatat aaagtaattc aaagggatgt tagcaaacta attgattttt tatatcgtta
     8041 ccaaaaaact tatttgaaac ttgataaagt aaaacgtaaa gaattttttt acgatcaacg
     8101 tacaaattat aatttacaaa ggtttaatat tgattatgat aaatattctg aaaattgggt
     8161 tcctcgtgct gcacaactaa tttttttaaa cagaacatgt tttaacggac tttacagggt
     8221 gaatttaaaa ggagaattta actcaccggt aggcgattat gataacccga caatttgtga
     8281 tgaacaaaat ttattggcag tcaataaggt tttagaaatt gccgaaataa aaaaagctga
     8341 ctttaaagat attgtaacag acttaagatc tgattctttt gtttatttcg atccacctta
     8401 taggccgata agtaaaactg caagttttaa agcatatcac aaacaaggtt ttactgatag
     8461 tgaacaattg cagttagcac agctttttaa acaacttgat tcagaaggat cttatgtcat
     8521 gttgagtaat tcagacccta aaaatattga tcctgatgat aattttttcg atgacatgta
     8581 taatggattc aatatagtaa gagttcctgc aagaagaatg ataaattcgg acccaaccaa
     8641 aaggggcaga ataaatgaaa tagtggtaac aaattacaca acagcataat ggaaaaagga
     8701 actaaaagtt atatatcagg caatcaactt gaagttgccg ttaaaacggt tttgatagga
     8761 aagggatttg agcttgtaaa atatagagat tgggagaaaa atcaagaaaa atatggtaag
     8821 gaattattac ttgaaaatgt tccttttaca acaatttatg agcacaacgg aaatacagaa
     8881 tttctgctta tttctgaaaa atatgaatta aaaattcgta tagaatgcaa atggcaacaa
     8941 gttggtggtt cagttgatga gaaactcccg tacctgtatt taaacacaat tgaagcaatg
     9001 ccagaagaaa caattatgat attaatagat ggctctggat ggaaaacagg cgcaattaaa
     9061 tggttaaagg atgccgttaa acagaaaaaa tatacaagcg aagagaataa acataaagaa
     9121 attttagttt ttagtttgac cgattttttt acttgggcta acaaaacatt taacagataa
     9181 aaagcacgaa cacacccccc ccctcgctcc cgccgatttg caatcggtgg tttgcttgtt
     9241 caagcaatca tattagcatt atgtctaatc atcctttcca tcataattca ttaattgcaa
     9301 aaattttcaa acaactcttt gcgtgaaaaa gattgtttgc ccgttgcaaa tcttattatt
     9361 tcattgctga tattcattat tgaattaaga ctgcaaatgt agatttattt ttttgaatta
     9421 agttctttca aattcattga aatatcactc aaaactctca atgaagccca aattgtcaat
     9481 gaacctaata acacacttac tgttgttaca aatcccattg ggttaaaaat taaatcttta
     9541 gtatatgtat attcggggtt ctgaaccata acaatcgtaa ataaacaaat tagagttcca
     9601 ataattccac cgattaatat aatggttgca ataactttta atgttttttc tgcagataaa
     9661 tttacttcct caatttcatc gctatttgta gtttcagaaa cctcatcaat tactttttct
     9721 tctaatgaat tatccataat atattttttt tagaattcct gctctaacgg ctttgcaggt
     9781 cttgcctttt gtcgttgcaa aagcagtaaa attgtgttgt ttttatactt gcaggcaacg
     9841 tttgatggta tggtgtcgtg cgggattacg aagcgatttc ctatcagttt
//
LOCUS       DAOSCH010000078         9839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4519_length_9839_cov_6.424877, whole genome shotgun sequence.
ACCESSION   DAOSCH010000078 DAOSCH010000000
VERSION     DAOSCH010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9839
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4519_length_9839_cov_6.424877"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLZ54_06615"
     CDS             <1..561
                     /locus_tag="PLZ54_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ66633.1"
                     /translation="ALIGKDAKSAGIGAAIGTAVGATTGAIIGNKMDKAAQEAAAIEG
                     AKVETITDINGLPAVKVTFESGILFETNKSTLNAASKDALTKFAQIMIQNPTMDIAII
                     GHTDNTGSLEVNQKLSQERAQAVANFLKSQGVPASQIKDVIGKNFSEPVADNSTAAGR
                     AANRRVEVYMYASEQMIKEAQQQSQK"
     gene            652..1017
                     /locus_tag="PLZ54_06620"
     CDS             652..1017
                     /locus_tag="PLZ54_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOJ66634.1"
                     /translation="MSAHNDIGNAGEERAQAYLREKGYKIRHTNWHAQKFELDIVAEK
                     DGLLIIVEVKTRSTENFEHPEDAVTPKKIRNIVQAAQEYIFQNNWEGETRFDIISVIP
                     SGQTFHIEHIEDAFLPPIN"
     gene            1125..1763
                     /gene="pyrE"
                     /locus_tag="PLZ54_06625"
     CDS             1125..1763
                     /gene="pyrE"
                     /locus_tag="PLZ54_06625"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOJ66635.1"
                     /translation="MKTLEKNIAEKLLKIKAIKLQPNNPFIWASGWKSPIYCDNRKTL
                     SYPSIRNYIKIELTRLIMELFPDVEVIAGVATGAIAQGVLVADALNLPFIYIRPTPKD
                     HGLENLIEGDLRPHQKVVIVEDLVSTGGSSLKAVEAIRNNGSQVLGMISIFNYGFPVA
                     EEKFKQAKVKHISLCNYKAILDQALTTNYITQEDMKTLQEWHKDPSAWGLES"
     gene            1780..2205
                     /locus_tag="PLZ54_06630"
     CDS             1780..2205
                     /locus_tag="PLZ54_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOJ66636.1"
                     /translation="MVTYESEIKKINSNAENIFNKFVDLQNFNKLINNKLSEDQIKDI
                     EFSEDSCSFSIEGFGRIGFRIIEKEPFKTIKFELRELPVQANAWIQLVEKAENDTRMK
                     LTFKAEIPAMLKIMVDKKLKEGVNLAAEAIANAFNNSNL"
     gene            2253..3587
                     /gene="argH"
                     /locus_tag="PLZ54_06635"
     CDS             2253..3587
                     /gene="argH"
                     /locus_tag="PLZ54_06635"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOJ66637.1"
                     /translation="MAKLWEKDLPLNKKIESFTIGKDKELDLFLAKYDVLGSIAHARM
                     LNAVGLLTAEETKLLIAELKKIYCSIENGEFKIEEGVEDVHSEVEFLLTDALGDTGKK
                     IHSGRSRNDQILLDLKLFTRAEIERTVRAVSSLFEVCQAQSERYKDILMPGYTHLQIA
                     MPSSFGLWFGAYAESLADDLQLLLAAWKITNRNPLGSAAGYGSSFPLNRQMTTDLLGF
                     DSMNYNVVYAQMGRGKMERIVSTALSGVAATLSKLAFDACLYNSQNFGFIKLPDEFTT
                     GSSIMPHKKNPDVFELIRAKCNKIQSVPQQILLITNNLPSGYFRDLQIVKEVFIPAFN
                     EMIDCIEMTKLMLEKIEVNVHILDDPRYDYLFSVEEVNQLATNGMPFRDAYKKVASEI
                     QNNSFRPQKNLHHTLEGSIGNLCNDQIKAYKEAILAQFTFEKVHQAEKALIE"
     gene            complement(3612..4901)
                     /locus_tag="PLZ54_06640"
     CDS             complement(3612..4901)
                     /locus_tag="PLZ54_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ66638.1"
                     /translation="MNNEKLSLQEFIDLFAQKANLKNKTAEDFIKNIVLVIEEALLKG
                     ESVKIKNFGVFKPQWIEPRKSVDVNSGKEIILPGSYKIGFVPDENLQNIVNEPFSYLE
                     AVELDIIDSSEKKEEETLEPLKIFSEQADEIKSIIAEIQSMSAHPDVKISESKEKQEK
                     IIPTENEDKKSEIFLEPEINNTDIVENESLKHLNDIENITSENVSEEKQEEKNNEIFD
                     KKSNHQKEDDFLEEFDEDTKSEPIGIPFQAYVPSEEYEFHENTEKKRYSWVWIPVVLM
                     LLLGGAGALYYFYPPVKNFVNLKMTKKSPILTERSLPSPAALTDTIQQQRQPEKVDSL
                     QILFDTPRAYSEFIAKERIRSGSRLARMAKRYYGASEFWVYIYEANRDKIPSPNDVPV
                     GTLIKIPKLDPRLIDVSNPRCMEKAKELSDLYLSLSK"
     gene            complement(4894..5184)
                     /locus_tag="PLZ54_06645"
     CDS             complement(4894..5184)
                     /locus_tag="PLZ54_06645"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOJ66639.1"
                     /translation="MNYKDFIQKVSTQTALTKEETEKLLETAINVFLEQLTENKSIGI
                     QGFGTFELRKKEERIIVHPATKKRLLMPPKLTVHFKSSNVLKEKLNNQLKNE"
     gene            complement(5206..6495)
                     /gene="rimO"
                     /locus_tag="PLZ54_06650"
     CDS             complement(5206..6495)
                     /gene="rimO"
                     /locus_tag="PLZ54_06650"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOJ66640.1"
                     /translation="MKKTVDVITLGCSKNLVDSELLMRQFEALGYQVRHDPQNPNGEI
                     VIINTCGFIGDAKEESIRTILQFAERRKKNKIDKLLVMGCLSQRYQNELQAEIPEVDK
                     FYGKFNFPEILTDLGQDFRADLRWERTLTTPSHYAYVKISEGCNRTCSYCAIPLITGR
                     HRSRPMEEIINEVQSLTSKGVKEFQLIAQDLSYYGADLYKSLKLPELVERLSDIPGVE
                     WIRLHYAYPAQFPYDLLKVMRERDNVCKYLDIALQHISDKMLKVMRRNFTKQETIELI
                     ETIRKEVPGIHIRTTMLLGHPGETEDDVEELKKFIKQTRFERLGAFAFSNEEGTYAFD
                     NYLDNIPEEEKLKRVDEIMAIQQKISAEINQTKIGKRLKVIIDRQEGENFIGRTEFDS
                     PEVDPEVIISDKVQGIEIGQFYLVEIVDADEYDLYAK"
     gene            complement(6552..7508)
                     /gene="ftsY"
                     /locus_tag="PLZ54_06655"
     CDS             complement(6552..7508)
                     /gene="ftsY"
                     /locus_tag="PLZ54_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOJ66641.1"
                     /translation="MGLINLFNKNKDKNENLEQGLQKTRESVFSKLSRAVIGKSKVDD
                     EVLDKLEEVLISSDVGVETTLRIIERIEKRVARDKYVNTNELNNILKEEIAALLSENN
                     TESFTDFNAELPAKPYVIMVVGVNGVGKTTTIGKLAYQFKQAGKKVYLGAADTFRAAA
                     IEQLVVWGQRAGVPVIKQQMGSDPASVAFDTLSSAKANDADVVIIDTAGRLHNKVNLM
                     NELSKIKNVMKKIIPDAPHEVLLVLDASTGQNAFEQAKQFTKATDVNALAITKLDGTA
                     KGGVVIGISDQFKIPVKYIGLGEGVDNLQLFDKNAFVESLFE"
     gene            complement(7696..8226)
                     /locus_tag="PLZ54_06660"
     CDS             complement(7696..8226)
                     /locus_tag="PLZ54_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOJ66642.1"
                     /translation="MKNRIEIVKGDITKLQADAIVNAANRTLLGGGGVDGAIHQAAGH
                     ELLEECKKLGGCNTGEAKITKGYHLPSKYVIHTVGPVWRGGDKNEPELLANCYRNSLL
                     LAKKYQIHSLAFPSISTGIYGYPIEKASVIALQEIAKFLKTEEIPEKVIIVCYSDHDY
                     QIYLQNFNEILTSEKN"
     gene            complement(8241..8936)
                     /locus_tag="PLZ54_06665"
     CDS             complement(8241..8936)
                     /locus_tag="PLZ54_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOJ66643.1"
                     /translation="MKTNFIVDFINLMYPNLCLICGENLMKNEDYLCMNCLHQIPKTN
                     YHLLPDNPIEKRFWGKADVFRATSFFYYQKGSLFQKLIHELKYNGNKEIGESLGKYAG
                     ADLISSADFSTVDMIVPVPLHPKKFAKRGYNQSEWIGKGLSSVMQKPQITNNLIRVKE
                     NTTQTKKSVYERFENTEGIFDLKDGLLFSGKHILLIDDVLTTGSTLEACVHALEKTEN
                     KNIKISIFTLAVA"
     gene            complement(8982..9362)
                     /locus_tag="PLZ54_06670"
     CDS             complement(8982..9362)
                     /locus_tag="PLZ54_06670"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOJ66644.1"
                     /translation="MNLNKILAGFIFAGRGIRTAFISERNLKIQTCFVVLVLLFGWLL
                     HIRIVEWLICLLCFGLVIGMELINTSIEKLVDLVSPEYHPLAEKVKDIAAGAVLFDSI
                     IAAIIGLIIFVPKIWEYLLRWISF"
BASE COUNT         2977 a         1837 c         1804 g         3221 t
ORIGIN      
        1 gcattgatag gtaaagatgc taaaagcgca ggaattggtg ctgcaattgg aactgctgtt
       61 ggagctacaa caggtgcaat tattgggaat aaaatggaca aagctgctca agaagctgca
      121 gctattgaag gtgctaaggt tgaaaccatt accgatatca atggattgcc tgcagtaaag
      181 gtaacttttg aatcgggaat tttatttgag accaacaaaa gtactttaaa tgctgcttcg
      241 aaagatgctt taacaaaatt tgctcagatc atgattcaga accccactat ggatattgcc
      301 attattggcc ataccgataa cacaggttcg ctggaagtta atcagaaatt atctcaggaa
      361 agagcacagg ctgtggccaa ctttttgaaa tcgcaaggtg ttcctgcttc tcaaattaaa
      421 gatgttattg gtaaaaattt ctctgagccg gttgccgata attctactgc tgccggcaga
      481 gcagccaatc gtcgcgtaga agtgtacatg tatgccagcg aacaaatgat aaaggaagct
      541 cagcagcaga gtcaaaaata aattcattat tttctaataa aagagctgct cttatatttt
      601 ggagcggctt tttttgtttg aatttccaaa ttttcgcaaa attttttcga tatgtcggct
      661 cataatgata ttggtaatgc aggagaagag cgagcgcaag cttatttgag agaaaaggga
      721 tataaaatca gacataccaa ttggcatgcg caaaaatttg aactggatat agtagccgaa
      781 aaagatggct tattgattat tgttgaagtt aaaacacgtt caacggaaaa ttttgaacat
      841 cccgaagatg ccgttactcc caaaaaaatc agaaatattg ttcaggcagc tcaagaatat
      901 attttccaaa acaattggga aggagaaaca cgcttcgaca tcatctctgt tattccttcc
      961 ggacaaactt tccatatcga acatatagaa gatgcttttc tacctccgat taattaattt
     1021 ctcaaacgat tggagaaatt ctgttttaac ttcttaaata ctttgcttaa ctttgcagaa
     1081 aaattaaaca gttttttgtt taccaatttt ataactatcc caaaatgaaa actttggaaa
     1141 aaaacattgc cgaaaaactt ttaaagatta aggcaataaa acttcagccc aacaatcctt
     1201 ttatttgggc ttcaggctgg aaatcaccga tttattgcga taacaggaaa acgctttcgt
     1261 atccttccat cagaaattat atcaaaatcg aattaacacg tttgattatg gaattgtttc
     1321 ccgatgtgga agttatagcc ggcgtagcta ccggcgcaat cgctcaggga gtgctggttg
     1381 ccgatgcgtt gaatctgcca tttatttata tccgccccac accgaaagat catggtttgg
     1441 aaaacctgat tgaaggtgat ttacgtcctc atcagaaagt agtgattgta gaagaccttg
     1501 tttcgacagg aggaagcagt ttgaaagctg tggaagctat tcgtaacaat ggctcgcaag
     1561 tattgggaat gatttccatt tttaattacg gattccctgt agctgaggaa aaattcaaac
     1621 aggccaaagt taagcatatt tccttgtgca attacaaagc cattctcgat caggcactga
     1681 ccaccaacta tattactcag gaagatatga agacattgca ggaatggcac aaggatcctt
     1741 ctgcatgggg attagaatct taattgaaag aaaaaattta tggtaactta cgaaagtgaa
     1801 attaaaaaga taaattcaaa tgcagaaaat atctttaata aatttgtcga tttacaaaat
     1861 ttcaataaac tgataaacaa taaattatct gaagatcaaa taaaagacat tgaattttcg
     1921 gaagatagct gttccttttc tatagaagga tttggcagga ttggttttag aattattgaa
     1981 aaagaaccgt ttaaaactat aaaatttgaa ttgcgtgaac ttccggttca agccaatgcg
     2041 tggattcaac tggtagaaaa agcagaaaat gatacacgca tgaaattaac attcaaggct
     2101 gaaatacctg ccatgttaaa aataatggtc gataaaaagc tgaaagaagg agttaatctg
     2161 gctgccgaag ctattgccaa tgcctttaac aattcaaatt tgtaagaatt ttactttata
     2221 ttcgtaactt caaaacattt agcttcatta ctatggctaa actctgggaa aaggatttac
     2281 ctttgaataa aaaaatagag tcgtttacga ttggcaaaga taaggaatta gacctatttc
     2341 tggcgaaata cgatgttttg ggttcgatag ctcatgcccg aatgctcaat gcggttggtt
     2401 tactcactgc tgaagaaacg aagttgttga ttgccgaatt gaaaaagatt tattgttcca
     2461 ttgagaacgg tgaatttaaa attgaagaag gagtggaaga tgtgcattcc gaagttgaat
     2521 ttttactgac agatgctttg ggagatacag gaaagaaaat tcacagtggc cgttcacgta
     2581 acgatcagat tttgttggat ctgaaattgt ttacacgagc tgaaattgag agaacggttc
     2641 gtgctgtttc ttcacttttt gaagtttgtc aggcgcaaag cgagcgttat aaggatattt
     2701 taatgcccgg gtacacccat ttgcaaattg ccatgccttc gtcttttggt ttgtggtttg
     2761 gcgcttatgc cgaaagtttg gccgatgacc tgcaactgtt gctggctgcg tggaagataa
     2821 ccaatcggaa tccgctggga tcagctgccg gctatggttc ttctttcccg ctcaatcgac
     2881 aaatgacaac cgatttgttg ggattcgaca gcatgaatta caacgtggta tatgcacaaa
     2941 tgggacgtgg aaaaatggaa agaatcgttt ctacggcttt gtccggtgtg gctgcaactc
     3001 tttccaaact ggctttcgat gcctgtcttt ataattctca aaactttggt ttcattaaat
     3061 tgcccgatga atttacgact ggctccagca ttatgcctca caaaaaaaat ccggatgtgt
     3121 ttgaactgat tcgagcaaaa tgcaataaaa ttcaatcagt acctcaacaa attttgttga
     3181 taaccaataa tttgccatca gggtatttcc gtgatttaca aattgttaag gaagtattta
     3241 taccggcttt caacgaaatg atagattgta tcgaaatgac gaaactgatg ctggaaaaaa
     3301 ttgaagttaa tgttcatatt ttggatgatc cacgttacga ttatcttttc agcgtggaag
     3361 aagtgaatca actggcaaca aacggcatgc cttttcgaga tgcttataaa aaagttgctt
     3421 ccgaaattca aaataattct ttccgaccac aaaaaaatct tcatcacaca cttgaaggca
     3481 gcattggcaa tttgtgcaac gatcagataa aagcttacaa ggaagcaatt ttagcgcaat
     3541 ttaccttcga aaaagttcat caggccgaaa aagctttgat tgaataatac agaaaatttt
     3601 cttcagcttt tttatttgga aagtgaaaga tacaaatcac ttagttcttt ggctttctcc
     3661 atgcaacgcg gattggaaac atcgattaat cgtggatcca gtttgggaat ttttatcagc
     3721 gttcctaccg gaacatcatt gggagaagga attttatctc tgttggcttc ataaatatac
     3781 acccaaaatt ccgaagctcc ataataacgt ttggccatcc gagccaatct gcttccactg
     3841 cgaatgcgtt ctttagcaat aaattcggaa taagctcgag gcgtatcgaa taaaatctgc
     3901 aaactgtcca ctttttccgg ctgtctttgt tgttgaatcg tatccgttag agcggcggga
     3961 gaggggagac tcctttcagt caaaattggc gacttttttg tcatttttaa atttacaaaa
     4021 ttcttcaccg gaggataaaa ataatacaat gcacctgctc cacccaataa aagcatcaga
     4081 acaacgggaa tccaaaccca tgaataacgt tttttttcag tattttcatg aaattcgtat
     4141 tcctccgatg gcacataagc ttgaaaagga attccgatag gttctgattt tgtatcttca
     4201 tcaaattctt ccaaaaaatc gtcttctttc tgatgattgc ttttcttatc aaaaatttca
     4261 ttgttctttt cttcctgctt ttcttcagaa acattttcag aggtgatatt ctctatgtca
     4321 ttaagatgtt ttagtgattc attttcaaca atatcagtgt tatttatttc aggctctaag
     4381 aatatttctg attttttgtc ctcgttttct gttggaatta ttttctcctg tttttctttg
     4441 ctttcgctga tttttacgtc cggatgtgca gacatggact gaatttcagc gataatgctt
     4501 ttaatttcat cagcctgctc gctaaatatt tttaagggtt cgagtgtttc ttcttctttt
     4561 ttttcagaag aatcgataat gtcaagttcc actgcttcca agtaagaaaa tggctcattt
     4621 acaatgtttt gtaaattttc gtcaggaacg aatccaattt tatagctccc cggcaaaata
     4681 atttcttttc cgctattgac atcaacactt ttacggggtt cgatccactg tggtttgaaa
     4741 acgccaaaat ttttaatttt taccgattct cctttcagca aagcttcttc tatcaccaaa
     4801 acgatatttt ttatgaagtc ttcggctgtt ttatttttta aatttgcctt ttgtgcaaac
     4861 aaatcgataa attcctgcaa acttaatttt tcgttattca tttttcagct ggttgttgag
     4921 tttttctttc aatacattgc ttgatttaaa gtgaaccgtg agttttgggg gcatgagcaa
     4981 tcgctttttt gttgccggat gtacaatgat gcgttcttct ttttttcgaa gctcaaaagt
     5041 tccaaatcct tgaataccaa tacttttgtt ttctgtcaac tgttctaaaa atacatttat
     5101 tgcggtttcg agcaatttct ctgtctcttc ctttgtaaga gcagtttgag tggaaacttt
     5161 ctgaataaaa tccttgtaat tcatgttttt agttttttca gatgattatt tggcatacaa
     5221 atcatattca tcggcatcga caatttccac cagatagaat tggccaattt ctatgccctg
     5281 taccttgtcc gaaattatta cttccggatc aacttccggc gaatcaaatt ctgtacgacc
     5341 gataaagttt tcaccttcct gtcggtcaat gataactttc agccttttgc cgattttggt
     5401 ttgattgatt tcggctgaaa ttttttgctg aatagccatt atttcatcaa ctctttttag
     5461 tttttcctct tcaggtatat tgtccagata attatcaaag gcatacgttc cttcttcatt
     5521 ggaaaaagca aaggcaccca atctttcgaa tcgggtttgt ttaatgaatt ttttcaattc
     5581 ttccacatca tcttccgttt ctccgggatg tcccaacagc atggttgtac gaatatggat
     5641 gccgggaact tcctttctta ttgtttctat cagttctata gtttcttgtt tggtgaaatt
     5701 tcttcgcatt actttcagca ttttgtcgct gatatgttga agtgcaatat ccagatattt
     5761 acaaacattg tcgcgttcac gcatcacttt gagcaaatcg taaggaaatt gtgcaggata
     5821 tgcgtagtgc agtctaatcc attcaactcc cggaatatcg gacagccgtt caaccagttc
     5881 cggtaacttt aacgatttat agaggtcagc tccataataa cttaaatcct gtgcaatcag
     5941 ctgaaattct ttaacccctt tgcttgtcag actctgaact tcattaatga tttcttccat
     6001 tggacgtgaa cgatgtcgtc cggtgatcaa aggaatggcg cagtaggaac aagtgcgatt
     6061 gcatccttcc gaaattttaa cataagcata gtgcgagggt gttgtcaggg tacgttccca
     6121 acgtaaatcg gctctaaaat cctgtcccaa atcggtcagt atttcgggaa aattaaattt
     6181 tccgtaaaat ttgtccactt caggtatttc ggcttgcaat tcattttgat aacgttgaga
     6241 caaacatccc attaccaaca atttatctat tttatttttt tttcttcttt cggcaaactg
     6301 aagaatggta cgaatgcttt cttctttggc atcgccgata aagccacaag tattgataat
     6361 aacaatttca ccgtttggat tttggggatc gtggcgaacc tgataaccca acgcttcaaa
     6421 ctgccgcatc agcaattcgg aatcgacaag atttttagaa caacccaaag taattacatc
     6481 aactgttttt ttcatgctga aattgaatcg gtaaacgaca ttattttaaa agtttggatt
     6541 atacgcaaaa atcactcgaa caaggattca acaaaagcat ttttatcaaa caattgcagg
     6601 ttgtcgactc cttcacccaa gccaatgtat ttcaccggga ttttaaactg atcggaaatt
     6661 ccaattacaa cgccaccttt ggcagtgcca tcaagttttg ttatagccag tgcattgaca
     6721 tcggttgctt ttgtaaactg ttttgcctgt tcaaaagcat tttgaccggt agaagcatca
     6781 agaaccagca atacttcatg aggagcgtcg ggaataattt ttttcattac gttttttatt
     6841 tttgacagct cgttcatcaa attcacttta ttgtgcaaac gtcctgccgt gtcgataatg
     6901 accacatcgg catcgttggc tttggccgac gaaagtgtat caaaagccac agaagccggg
     6961 tcggaaccca tttgctgctt gataaccgga acgccggcac gctgtcccca aactaccaac
     7021 tgttcaatgg cagcagcacg aaaagtatcg gcagctccca aatatacttt tttaccagcc
     7081 tgcttaaact gataggcaag ttttccgata gtagtggttt tccccactcc atttacgccc
     7141 acaaccatga tcacataagg ttttgcgggc aattcggcat taaaatcggt aaaggattcc
     7201 gtattatttt cagaaagaag tgctgcaatt tcttctttca aaatgttgtt tagttcattg
     7261 gtattcacat atttatcacg agccacacgt ttctctattc tttcaatgat tcgcaaagtg
     7321 gtttcaacgc ctacgtcgga actgataaga acttcctcca gcttatcgag aacctcatca
     7381 tccactttcg attttccgat tactgcccgt gataatttgg aaaaaacgct ttctctggtc
     7441 ttttgtaatc cttgttccaa attttcgttt ttatccttgt ttttattgaa taaattgata
     7501 agtcccattc ttttgctttt taaatttcaa tttacaaaat tataacattt ttccgagttt
     7561 tccgattttg gaacaacata tccacaaaga cagaaataca aacaattgga tacaaataat
     7621 ttagtagaat atgcagtgtt cagcaatttt tattgtcaga aaaggctgga aaatggagtg
     7681 aaagagaaga gaaaatcaat ttttttcgga agttaatatt tcattgaagt tttgcagata
     7741 gatttgataa tcatggtcgc tataacaaac aataatgact ttctcgggta tttcttcggt
     7801 ttttaagaat tttgcaattt cctgcaacgc aatgacactt gctttttcta tgggatagcc
     7861 gtaaatgcct gtactaatag aaggaaaagc taaagagtga atttggtatt tttttgctaa
     7921 aagcaaactg tttctataac agttagccaa taattccggt tcatttttat ctccgcctcg
     7981 ccatacaggt ccaacggtat gaataacgta ttttgaaggg agatgatatc ctttggttat
     8041 ttttgcctcg ccggtattgc atcctccaag ctttttacat tcttccaata gttcatgccc
     8101 ggcagcttga tgaatggcac cgtccacacc tcctccaccc aacaaagttc gattggcggc
     8161 atttactatg gcatctgcct gcagttttgt aatatcgccc ttcacgattt ctattctgtt
     8221 tttcataatt gtgattaaat ttaagcaact gctaaagtaa aaatgctgat tttgatattt
     8281 ttgttttcag ttttttcgag tgcatggacg caggcttcca atgttgagcc tgtagtcaat
     8341 acatcatcaa tcagcaatat gtgttttccc gaaaacaaca agccatcttt caaatcaaaa
     8401 attccttcag tgttttcaaa cctttcgtaa accgattttt tggtttgggt ggtattttct
     8461 tttacgcgaa ttaaattatt ggtgatttgt ggtttttgca taacgctcga caaaccttta
     8521 ccaatccact cgctttggtt gtaacctctt ttggcaaatt tttttggatg caaaggtacc
     8581 ggtacaatca tatctactgt ggaaaaatcg gctgaactta tcagatctgc tccggcatat
     8641 tttcccaacg attcgccaat ttctttgttt ccattgtatt tgagttcgtg aataagtttt
     8701 tgaaacagtg atcctttttg ataatagaag aaagaagtag cccggaacac atcggctttt
     8761 ccccagaaac gtttttcaat aggattgtcg ggcagaagat gataatttgt tttaggaatt
     8821 tgatgtaggc aattcatgca aagataatct tcgtttttca tcaaattttc tccacaaata
     8881 aggcaaagat tcggatacat gaggttgata aagtcaacaa taaaattggt tttcataagg
     8941 ctgtagtttc ggataaaaac attacgaaaa ttagacttta ctcaaaatga aatccagcga
     9001 agaagatatt cccaaatctt aggtacaaaa attatcaatc caataattgc cgcaataata
     9061 gaatcaaaca atacggcgcc tgcagcaata tctttgactt tttccgccaa gggatgatat
     9121 tcgggtgaaa ccaaatcgac cagtttttct atggaagtat ttatcagttc cataccgatg
     9181 accagtccaa aacacaaaag acaaattaac cattctacga tccgaatatg caacaaccat
     9241 ccgaaaagta agaccaacac aacgaagcag gtttgaattt tcaaatttct ttccgaaatg
     9301 aatgcagtac gaattcctct tccggcaaaa ataaatccgg caagtatttt attcaggttc
     9361 atgttaacaa aaatgtgttc tgattcacaa aaattttaat acatgacaaa aattgaaaca
     9421 tttgttttat atatctgtaa aacagaacat ataagccaag ctcttcgggt tgaccttgtc
     9481 cggtgagccg aaaaacaagt gagaaaagcg tttaaaaatc gtccttgttt gagttccgac
     9541 gtatttcgtc ggaatgagtt tggacgattt cagcttttcg agcgtaagtt tttcgagtga
     9601 agtgggcaca gtcttgaatt tttttgctta cttttttgtt tcaagacaaa aaagtaagtg
     9661 gggttcgggg caaagcccct ttgttaaata tctgaaaata aataatataa atatttgtac
     9721 aattttttgt catgtactaa gcacaaaaat ataaaaaaat ctgttgaaca aatttgttca
     9781 acagattttt tacaggaaat atttttaaaa tttcttttaa attctatcgt tacactgtt
//
LOCUS       DAOSCH010000079         9743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4573_length_9743_cov_6.978840, whole genome shotgun sequence.
ACCESSION   DAOSCH010000079 DAOSCH010000000
VERSION     DAOSCH010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9743
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4573_length_9743_cov_6.978840"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(193..855)
                     /locus_tag="PLZ54_06675"
     CDS             complement(193..855)
                     /locus_tag="PLZ54_06675"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S single cluster domain-containing protein"
                     /protein_id="HOJ66645.1"
                     /translation="MNIAHFEEHSFIYGPGCRFVIWVQGCSIRCKGCWNKEMWAFEDR
                     IILSVKDLLLKIKNEKELIEGITLLGGEPLDQFDEIYELLVECKKLGLSTMLFTGYEM
                     TEIIEKKMSSLLDNLDILITGRYDKTKRTMNHQWIGSTNQEIHFLTDRYADYQIENNN
                     YTEITINEDASLTILGFPQLKTFEYETNLIANTLPKPHVPALKPKLCQRACKANAPRE
                     IV"
     gene            complement(852..1361)
                     /locus_tag="PLZ54_06680"
     CDS             complement(852..1361)
                     /locus_tag="PLZ54_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66646.1"
                     /translation="MSAVVKTATPFINKELLLQALKNIGCNFTLQGNDILTDRKDYYG
                     FQKFSLNNGRYVFLHDSSAEIGSFYMTQPNGYPWGNIKNQEYKSVSEFLKTVENEYQS
                     LYQKKLKELELKRLEEEKRRLEQERIAFIEKQKSTIIARAKEKGYSVKEENVKGKIKL
                     VLVRNTYSK"
     gene            complement(1358..1564)
                     /locus_tag="PLZ54_06685"
     CDS             complement(1358..1564)
                     /locus_tag="PLZ54_06685"
                     /inference="COORDINATES: protein motif:HMM:NF022657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HOJ66647.1"
                     /translation="MAEQRIVITIDENGIINASTEGIKGEMCLDELQKLLSELADLDS
                     ITKTDEFYQGNELKVQNKIQNKKQ"
     gene            complement(1569..3350)
                     /locus_tag="PLZ54_06690"
     CDS             complement(1569..3350)
                     /locus_tag="PLZ54_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66648.1"
                     /translation="MANKIQPISKTKTVDYSNELLQNFWRQKYNALANQNHKNEIAKT
                     LQLVQEGIVCIQTESFTDKHLIKQIFEIVQNFKVRFYILVNDYSQELDLLNEICLIRY
                     EVKNVGSFILVNPNSDYSQGIFFTGQLTEQSLAVTQHISQSLKPKETEELFRHFCFHF
                     WETAKKEVIEKGKHNDVTSKPIDVFHDINAFAEKDFVYGTVFDFSEKSKRIDLSEKQI
                     IYLGQETQIPIQIKSKSTKDLGDFVLDELIPKNEFEDKEPNLTDDGISVSIEYKWQNV
                     PFYLPEEAIESALYERWKKKQEEIVKALDSILNKIQEAEKKEANLSKAISRFFLGKKN
                     VFGALKNEIEDLKAADYANLPEAKLRVKINRINEVNAQVQKEIGEIETEDRKAKLDEE
                     IESLKTQISELESELKSKKQELEIKKSNMDKALEEFCLKYGIDKNKLGQEKNNLGQLA
                     GEKNRKKNPNEAKEAEKKLNELKEIQNTTFISKTESEINEIEKKINSLKNQIGGKEKE
                     KNKQVSQSSSSSLSEVVGDKNVIQSNSIKSQLVQLPDQFPKLPKVGKLYQLNNQYFLA
                     IEFWEEYELGKKEAERLKANLCAIKNQ"
     gene            complement(3340..4608)
                     /locus_tag="PLZ54_06695"
     CDS             complement(3340..4608)
                     /locus_tag="PLZ54_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66649.1"
                     /translation="MFVSEEGKYQIWFTQNDSFFKNIILFFRRIQPNAKDEIQNKISL
                     DKKGHFLLPTDENKYTKLNLISDEIYGNLQNCNDQIHFSWVWENLKKSYYVFSGQLGN
                     KDKPTKIKSEAIPSDKDIQRIISDVLPEWNNEKNRYSIRFDQLKEESKRTFEEKHYEC
                     EWEGFKIQIQNLPLMPYDKEEAKKWRNWLLNEELKKEYFSMSDFDEEAIELNEKEALA
                     AFSLDIPKIKDYIEKTESKKVFWHLQAPIDLNPNAKIKLSAKPVELKQGDKLSFADIV
                     AKLGLENCSVFVYYDRYVYNHNQQRAVSALAKAINSPKKIVITDLTSKENISDFIQKN
                     VKEIKLRDSKTIFKGRSPHDRYLITSNQNEMSIWNISNSIDYISFSDYNINESIVGTI
                     RQSVVFTPISKEMLDKDLLNFINNEVKNGK"
     gene            complement(4830..8000)
                     /locus_tag="PLZ54_06700"
     CDS             complement(4830..8000)
                     /locus_tag="PLZ54_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HOJ66650.1"
                     /translation="MQFYYFNLLKDHELHFEKNKIVSSAIINTARRALEPFEMYLQNA
                     ITALEKKQNIKAKWKKIKTENIAELRLIGKLFEVPKQTFVQIDESIANDIKKETVIRG
                     SVNDHRVVSIDGSNIVFDILPEQDEMLFWNKKTIEYKIIPASKPKGTIIREEQNRYIV
                     YSENGQKPNDNASEIQNRKLASFIDFENLRFENGQILNATRNNDLNITLSDINDFNKI
                     VICDKLKFKIENTRKNSKDAFWIQLLELDDNADDDIPGFSTLKYFFDDGIDIEDDQKN
                     KYQLGFGIESENRIVLKKKNDKGYWQFCIPPEDSILTVKVNTYQLRKQIEAISTLMRM
                     PVGEHYKLIKLFENREKTKWPSPLKERIEEWFVITDETRSGTKEQRQFIHQAINTPDF
                     AILEGPPGSGKTTVILELICQLVKRGKRVLLCGSTHVAIDNVLERLKEKRNNISLLEQ
                     FHILPVRIGDEHRISDNVKEFQINNLIEENSIDESLLLDISNLVCGTTIGILQHPKFK
                     QRKGTLKKDEKEGRLMYSSSEPIVPEFDYLIIDESSKTTFQEFLVPALYAKKWILAGD
                     IMQLSPFTDREEIVSNIEQLSVDGKPISNELQQAVFYLQKIKDCLREKHNKFVLPVSL
                     ETLNYILLELFNGRNEDYKNRGICVVVGNNQEEAKNKLLVKRKIKNVSKIELGAYDMI
                     IIEDHLLEKNLHLIPESHAVLRSKKWQESEHAFVHNVYQQKHSFHYKEKGREFENSFE
                     IVESVNTYFAEKSWAEEIAWRIDREHQIRLTNNSKLKGSYGKAIEELIPKSLNKEKIE
                     EKINTIASMAFPSILESLVKGISGRKLKFESTISEGFNQCDISHRKTTLRYQHRMHPD
                     ISKFPREQFYQSENALLDLEKPKHIKNLRQWDFDKYPYRNIWIDVKGNAFEGKNHDEV
                     KVMIKELKEFVEFAKNNVQPEGKEWSVACLTFYRGQETLLREELQKLTENENGYSNFN
                     ITSGKYRINIKLHTVDKFQGQEADVVFLSMVQTNRDGFLDNPNRLNVAITRAKFQLVV
                     IGSYDYYTKKSNSEDLKALAKNTHRL"
     gene            complement(8002..9558)
                     /locus_tag="PLZ54_06705"
     CDS             complement(8002..9558)
                     /locus_tag="PLZ54_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ66651.1"
                     /translation="MENDLKNLIKANVKLIQIISFESLRIHGMLVRTANELEKDLFIW
                     NRVEGLKKWDKEKRSFEFEDEEKQLPDTVIDFFKEKENLILLLEDFYPDLTEDKPRMI
                     RTFRNIALENSKGKTLVFSQPFLQLPKELEKDVHILELPYPCFEDIKAIYKKVCKEYN
                     IPNADNPDHELIESALGLTIMEAEKAFALAYIENGSLSGAEVPLIIREKENVIKKSGY
                     LEYYHPKEEMKDVGGLANLKDWLTKRGRGFDKGAKDFGLTYPRGILLLGIPGTGKSLT
                     AKAVGNLWHFPLLRLDMGKIFGGIVGESESNIREALKIAEAIAPSILWIDEIEKGMSG
                     ISSSGSTDGGTTARVLGTFLTWLQEKTKPVFVVATANDISQLPPELLRKGRVDEIFFV
                     DLPTVEEREEIIKIHLKKKNRQAQNFDIKKLAKASKGFSGAELEEVIKEALFQAYDEE
                     KEVTDEHILAAIEKTFPLSRTMHETIDKMRLWAKSRAVPASTNEPETLDDNGKDVPKL
                     KQESYNNPFI"
BASE COUNT         2658 a         1799 c         1443 g         3843 t
ORIGIN      
        1 gatgctgaaa cgctgaaacc cgcactatct cggcaataaa atataccgcc tgttaccgcc
       61 tggtgttctg tcttccagtc ttgcaaacca ttgtcattat tcagttttcg tgtcattgtc
      121 attttacttt cttccttttc agtcggcttg tgtgtcagca ttttttattt tttttaatgt
      181 cggcaaattt ttttaaacaa tttctctcgg tgcgttggct ttgcaagctc tttgacaaag
      241 ctttggtttt agcgctggta cgtgtggctt tggcaatgtg tttgcaataa ggttggtctc
      301 atattcaaat gttttgagtt gtggaaatcc taaaattgtc aaactagcat cctcgttaat
      361 tgttatttct gtatagttat tattttcaat ttgatagtct gcgtatcggt ctgtcaaaaa
      421 atgaatttct tgatttgttg aaccaatcca ttgatgattc attgttcgct tagttttgtc
      481 gtaacgccct gttatgagaa tgtctaagtt gtcaagcagt gatgacattt tcttttctat
      541 gatttctgtc atttcgtaac ctgtgaaaag catagtgcta agtccgagct ttttgcattc
      601 aacaagcaat tcataaatct cgtcaaattg atctaatggt tcgcccccta ataaagttat
      661 tccctcaata agttcttttt cgtttttaat tttgagtagt aaatccttaa cggataaaat
      721 tattctgtct tcaaaagccc acatttcttt attccaacaa cctttacaac gaatagaaca
      781 gccttgtacc cagattacga atctgcaccc tggtccataa ataaaactgt gttcttcaaa
      841 gtgggcaatg ttcattttga gtaagtgttt cttacaagta ctaatttaat tttacctttt
      901 acattttctt cttttactga atagcctttc tctttggctc tcgctataat tgttgatttt
      961 tgcttttcaa taaaagctat acgttcttgc tctaagcgtc tcttttcttc ttcaagacgt
     1021 tttaattcta attctttaag tttcttttga taaagtgatt ggtattcgtt ttcaactgtt
     1081 tttagaaatt cgcttacaga tttatactct tggtttttaa tgtttcccca aggataacca
     1141 tttggttgtg tcatgtagaa tgaacctatt tctgcgcttg aatcgtgaag aaatacatat
     1201 cgtccattgt tcaatgaaaa cttttggaaa ccgtaataat cttttcggtc tgttaaaata
     1261 tcgttacctt gtaatgtaaa attgcaacca atatttttaa gagcttgcaa caacaattct
     1321 ttattgatga atggtgttgc tgttttaact actgcactca ttgtttttta ttttgaattt
     1381 tgttttgaac ttttaactca tttccctgat aaaattcatc agttttagtt attgaatcca
     1441 aatcagctaa ttcgcttaga agtttttgca attcatcaag gcacatttcg cccttaatac
     1501 cttcagttga agcatttatt atcccatttt cgtcaattgt aataacaatt ctttgttctg
     1561 ccataatatt attggttttt aattgcacat aaattagctt ttagtctctc cgcttctttc
     1621 ttacctaatt cgtattcttc ccaaaactct attgccaaaa aatattgatt atttaactga
     1681 tacaatttcc cgacttttgg cagctttggg aactgatcag gcaactgaac taattgagat
     1741 tttatggaat tactttgtat tacatttttg tctccgacaa cttcactcaa cgaagaggaa
     1801 cttgattgag aaacttgttt attcttttct ttttcctttc ctccaatttg gtttttcaag
     1861 gaattgattt ttttctctat ttcatttatt tcgctttcag tttttgaaat aaaggttgtg
     1921 ttttgaatct cctttaactc gtttagtttt ttttcagctt ctttagcctc gttgggattt
     1981 ttctttctat tcttctcacc tgccaactgc cctaaattat ttttctcttg cccaagttta
     2041 tttttatcaa taccatattt cagacaaaac tcttcaagag ccttatccat attagacttt
     2101 ttaatttcta attcctgctt ctttgatttt aattcgcttt ctagttccga aatctgagtt
     2161 tttaaacttt caatttcttc atcaagtttt gctttacggt cttcggtttc tatttcgcca
     2221 atttcttttt gaacttgtgc attaacttca ttgatacgat tgattttcac tcttaacttt
     2281 gcttccggta agtttgcata atcggctgct tttaaatctt caatttcatt tttcaatgca
     2341 ccgaagacat ttttcttacc caaaaaaaag cgtgaaatag ctttcgataa attggcctct
     2401 ttcttttctg cttcctggat tttattcaaa atagaatcca acgctttaac aatttcttct
     2461 tgttttttct tccacctttc ataaagagca ctttcgattg cctcttcagg taaataaaat
     2521 ggaacatttt gccatttgta ttcaatgctt acagaaattc cgtcatcagt taaatttggt
     2581 tctttatctt caaactcgtt tttcggtatc aactcatcta aaacaaaatc gcctaaatcc
     2641 ttagtgcttt ttgattttat ctgaattgga atttgtgtct cctgcccgag ataaatgatt
     2701 tgtttttcag ataagtcgat tcgttttgat ttttctgaga aatcaaatac ggttccgtac
     2761 acaaaatctt tttcagcaaa agcgttaata tcgtgaaaaa cgtcaatcgg ttttgaggtt
     2821 acatcattgt gtttcccttt ttcaatcact tcctttttgg cggtttccca aaaatgaaaa
     2881 cagaaatgac ggaataattc ttctgtttct ttgggtttca aactttgtga aatgtgttgt
     2941 gttacagcta aactttgttc agtgagttgt cccgtaaaaa atattccttg tgagtagtcc
     3001 gaattgggat ttaccaaaat aaaagaacca acgtttttca cttcgtaacg aatcaaacaa
     3061 atttcattca gcaaatccaa ttcctgagaa tagtcattaa caagaatgta aaaacgcact
     3121 ttaaaatttt gaacaatttc aaaaatctgt tttatcaagt gtttgtcggt aaatgattca
     3181 gtttgtatgc atactattcc ttcttgcacc aactgtagtg tttttgcaat ttcgtttttg
     3241 tgattttgat ttgctaacgc gttgtatttt tgtcgccaaa aattctgcag taactcgttg
     3301 ctgtaatcaa cagtttttgt tttcgatata ggttgtattt tatttgccat ttttaacctc
     3361 gttattaatg aagtttaata aatctttatc gagcatttct ttactgattg gggtaaagac
     3421 aacgctttgt cggattgttc caactatgct ttcgttaatg ttatagtctg aaaatgaaat
     3481 gtagtcaata gaattgctaa tattccaaat gctcatttca ttttgatttg aagtaattaa
     3541 atatctgtcg tgtggtgaac gtcctttaaa aatggttttg gaatccctca atttgatttc
     3601 cttcacgttc ttttgaataa aatcacttat attctctttg gatgttaaat cagtaatgac
     3661 aatttttttc gggctgttta tggcttttgc aagtgctgaa acagctcttt gctgattgtg
     3721 gttgtatacg tatcggtcgt aatacacgaa aaccgaacag ttttctaatc ctaatttagc
     3781 aacaatatca gcaaacgaaa gcttgtcgcc ttgctttaat tcgactggct ttgcagatag
     3841 tttaattttt gcattcgggt tcaaatcaat gggggcttgt aaatgccaaa acactttttt
     3901 cgattcagtt ttttcaatat aatcttttat cttgggaata tctaaactaa aagccgctaa
     3961 tgcttctttt tcgtttaatt caatagcttc ttcatcaaaa tcgctcatgc taaaatattc
     4021 ctttttcaat tcttcattca ataaccaatt ccgccatttt tttgcctctt ctttatcgta
     4081 aggcattaaa ggcaaatttt gaatttgaat cttaaaccct tcccattcac attcataatg
     4141 tttttcttca aacgttctct tgctttcttc tttcaattgg tcaaaacgaa ttgaataacg
     4201 atttttctca ttattccact ccggcaagac atctgagatg attctttgaa tgtctttatc
     4261 actcggaatt gcttcgcttt ttatctttgt aggtttatct ttatttccca gctgaccgct
     4321 aaatacgtaa tacgattttt ttaaattttc ccaaacccag gaaaagtgaa tctggtcatt
     4381 acagttttga agattcccgt aaatttcatc tgaaatcaaa ttcaactttg tgtatttatt
     4441 ttcgtctgta ggcaaaagaa aatgcccttt tttgtcaagg ctaattttat tttgaatttc
     4501 atcttttgca tttggctgta ttcttctgaa aaataaaata atgtttttaa agaaagaatc
     4561 attttgtgta aaccaaattt gatacttacc ttcttcggat acaaacattt ttccagtttc
     4621 ttttactgca tttccatgtt ttgtaagttg gtattgttca tctaaaacac caatgttttt
     4681 cagataatcc ttaatccgat tttcaataag cgattcaaaa cgtttttcgc cattgagata
     4741 ttcctgaatg tctttacgca attcttcttt ctgaaaacgt aaatacgcat gtgcagaata
     4801 gagtttaatg tttttattta aagttacttt cataatctat gtgtgttttt ggctaatgct
     4861 tttaaatctt ccgaattgga ttttttagtg taataatcgt agctgcctat aacgaccagc
     4921 tgaaactttg ctcttgttat tgccacattc aaacggttag gattatccaa aaatccatcc
     4981 cggttagttt gaaccattga aagaaaaaca acatcagctt cttgtccttg aaatttatct
     5041 actgtgtgca atttaatatt tattctatat tttccgcttg tgatattaaa attggaataa
     5101 ccattttcgt tttctgtgag tttctgtaat tcttcacgta gcaatgtttc ttgtcctctg
     5161 taaaaagtta aacatgcaac tgaccattct ttgccttcgg gttggacgtt attttttgca
     5221 aactctacaa actctttcag ctctttaatc attactttta cttcatcatg atttttccct
     5281 tcaaaggcat ttccttttac atcaatccaa atattacgat atggatattt atcaaaatcc
     5341 cattgcctta aatttttaat atgttttggt ttttccagat ctaacaaagc attttccgat
     5401 tgataaaatt gctctctcgg aaactttgaa atatccgggt gcatacgatg ttgatacctc
     5461 aacgtagttt tacgatgcga aatgtcgcat tgattaaacc cttcagaaat agttgactca
     5521 aatttcaatt ttctgcccga aatgcctttt accaaggatt ccaaaattga cggaaacgcc
     5581 attgaagcaa ttgtgttaat tttttcttca attttctctt tgtttaatga ttttggaata
     5641 agttcctcaa tcgcttttcc gtagctgccc ttgagtttgc tattattagt aagtcgaatt
     5701 tgatgttctc tatctatacg ccatgcaatc tcttctgccc agcttttttc agcaaaataa
     5761 gtatttactg actcaacaat ttcaaaactg ttttcaaact cacgtccttt ttctttataa
     5821 tgaaatgaat gtttttgctg ataaacatta tgaacaaatg cgtgttcgct ttcttgccac
     5881 tttttacttc ttaaaactgc gtggctttcc ggtataagat gtaagttttt ttcaagaaga
     5941 tgatcttcta ttataatcat gtcgtacgcc cctaattcta ttttgcttac atttttgatc
     6001 tttcgtttta caagcaattt attttttgct tcttcttgat tgttgccaac aactacacaa
     6061 attcctctgt ttttatagtc ctcattacga ccattaaaaa gttctaataa aatataatta
     6121 agtgtttcca atgaaacagg taaaacaaat ttattatgct tttctcgcaa acaatctttt
     6181 attttttgca aatagaaaac tgcttgttgt aattcgtttg aaattggctt gccgtcaaca
     6241 cttaattgtt caatgtttga aactatttct tctctatctg taaatggcga aagctgcatt
     6301 atatcacctg ctaaaatcca ttttttagca tataaagcag gaacaagaaa ttcttgaaaa
     6361 gtagttttac tactttcatc aattatcaaa taatcaaatt ccggaacgat tggctctgag
     6421 ctggaataca ttagtctgcc ttctttttca tcttttttaa gagtcccttt tcgctgtttg
     6481 aattttgggt gttgcaagat tccgatggtt gtaccacata ccaaatttga aatatcgagc
     6541 aataaacttt catcaattga attttcctca attaaattgt taatctgaaa ttccttaaca
     6601 ttatcgctaa tacgatgttc atctccaatg cgaacaggta atatgtgaaa ttgttccagc
     6661 aaagaaatat tgtttcgctt ttcttttaac cgttctaaaa cattgtcaat agcaacatgt
     6721 gttgagccac aaagtaaaac tcgtttacct cgcttaacta attgacaaat caattctaag
     6781 ataacagtag tttttcccga ccctggaggc ccttccaaaa tggcaaaatc tggagtattg
     6841 attgcctgat gaataaattg gcgttgttct ttagttccac ttcttgtttc gtctgtaatt
     6901 acaaaccatt cctctattct ttcctttaat ggagacggcc atttagtttt ttctctgttt
     6961 tcaaagagtt taatgagttt gtagtgctca ccaacaggca ttcgcataag cgtactaatg
     7021 gcttcaattt gtttgcgtaa ctgatacgta tttactttta cagtaagaat tgaatcttca
     7081 ggcggaatgc aaaattgcca gtatccttta tcatttttct tttttaaaac aatacgattc
     7141 tctgattcaa taccaaaacc caattgatat ttattttttt ggtcatcttc aatatcaatt
     7201 ccatcgtcaa agaagtattt taatgtcgag aaacctggaa tatcatcatc agcattgtcg
     7261 tctaattcca aaagttgaat ccaaaaagca tctttgctgt tttttcgagt gttctctatt
     7321 ttgaatttca atttatcgca gataacaatt ttattgaaat catttatgtc ggaaagtgtg
     7381 atgttcaaat cattgtttct tgttgcattt agtatttgac cgttttcaaa cctcagattt
     7441 tcaaaatcaa taaaacttgc taattttcga ttttgaattt cacttgcgtt atcatttggt
     7501 ttttgtccgt tttcagaata gacaatgtag cgattttgtt cctctctgat aatcgttccc
     7561 ttgggcttgc ttgccggaat gattttatat tcaattgttt ttttgttcca aaataacatt
     7621 tcgtcttgtt cgggaagaat atcaaaaaca atattagagc catcgataga aacaactctg
     7681 tggtcgttaa cactacctct gattactgtt tcttttttga tatcgtttgc aatactctca
     7741 tcaatttgaa caaatgtttg tttaggcact tcaaataact ttccaatcaa acgcaattca
     7801 gcaatatttt ccgtttttat ctttttccat tttgccttga tattttgttt tttctctaaa
     7861 gctgtaatgg cgttttgcaa atacatttca aaaggttcta acgctcgtct tgcggtattg
     7921 attatcgcag acgaaacaat tttgtttttt tcaaaatgta gttcgtgatc tttaagtaaa
     7981 ttgaaataat agaactgcat gttaaataaa aggattgttg taggattctt gtttcaattt
     8041 aggaacatct ttgccgttgt catccaaagt ttctggttcg tttgttgatg ctggtacggc
     8101 ccggcttttt gcccacaaac gcatcttgtc aattgtttcg tgcatcgttc gtgaaagcgg
     8161 aaatgttttt tcgattgctg ctaaaatatg ttcatctgta acttcttttt cttcatcata
     8221 agcctgaaat aatgcttctt ttataacctc ttccaattct gcaccagaaa aacctttgct
     8281 tgcttttgca agttttttta tgtcaaaatt ttgggcttgt cggttttttt tcttcaaatg
     8341 tattttaata atttcttcac gttcttctac agttggcaag tccacaaaga aaatttcatc
     8401 aacacgccct ttacgaagta attctggagg taattgcgaa atatcgtttg cagttgcaac
     8461 cacaaaaaca ggttttgttt tttcttgtaa ccatgttaaa aatgttccta agactcttgc
     8521 ggttgttcct ccgtcagtac tacccgaaga tgaaatacct gacattcctt tttctatttc
     8581 gtcaatccac aagattgaag gagcaattgc ttcagcaatt tttaatgctt cacggatatt
     8641 gctttctgat tcacccacaa tcccaccaaa gatttttccc atatccaaac gtaataatgg
     8701 gaaatgccac aaattaccaa ccgctttagc cgttaaactt tttcctgtgc caggtattcc
     8761 caacaataaa atcccacgcg gataggtcaa tccaaaatcc ttagcacctt tgtcgaaacc
     8821 tctgccacgt ttggttagcc aatcttttaa attcgccaac cccccaacat ctttcatttc
     8881 ttccttcgga tgatagtatt cgagataacc acttttttta attacgtttt ctttttctcg
     8941 aattatcaat ggaacttctg ctccggataa actgccgttt tcaatataag ctaaagcaaa
     9001 tgctttttca gcttccataa tcgttaatcc cagcgctgat tcaatcaatt cgtgatcagg
     9061 attatcagca ttgggaatat tgtattcttt acatactttt ttataaattg ctttaatatc
     9121 ttcaaaacat ggatatggca attctaaaat atgaacatct ttttccaact ctttaggcaa
     9181 ttgcaaaaag ggttgtgaga aaactaatgt ttttcccttt gaattctcaa gtgcgatgtt
     9241 tctgaaagtt ctaatcattc gcggcttgtc ctctgtcaaa tcagggtaaa aatcttctaa
     9301 taataaaatc aagttttctt tttctttgaa aaaatcaata acggtatcag gaagttgttt
     9361 ttcttcgtct tcaaattcaa aacttcgttt ttccttatcc cactttttta atccttcaac
     9421 tctgttccaa atgaataagt ctttttccaa ttcattagca gttctgacta acatgccgtg
     9481 tattcttagt gattcgaaac tgattatttg tatcagtttg acattcgctt tgataaggtt
     9541 tttaagatcg ttttccatgt gtatttattt tggttttaat cgtgtgtcag ctagattgca
     9601 ctctttgaca aagctttggt tgtagcgctg gcttgtgtgg ctttggcaat gagtgcaatg
     9661 tgggtcggca attgtttaaa aaaatgtgcg gtgggcaaaa aataaaaaac accgggtcgg
     9721 cttggatgtc ggctttctgg tct
//
LOCUS       DAOSCH010000080         9315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4855_length_9315_cov_6.391685, whole genome shotgun sequence.
ACCESSION   DAOSCH010000080 DAOSCH010000000
VERSION     DAOSCH010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9315
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4855_length_9315_cov_6.391685"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PLZ54_06710"
     CDS             complement(<1..699)
                     /locus_tag="PLZ54_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOJ66652.1"
                     /translation="MIVYKFGGASVRSAAGIKNLASIVSGVNDNLFIVVSAMGKTTNA
                     LEDVLEDFMQADHASALAKFQQIENYHNEILAELFPDTENPVAILQPLYDEITSMLKL
                     SVGDDYDRWYDKIVSYGEIISTQIVSKFLNETGIQNRWLDMRKLLITDSNFREANVKM
                     KESQQKLTEAANFENYPVYIGQGFIGANTKGDPTTLGREGSDYTAAVVANLLNAENLT
                     IWKDVPGILNADPRL"
     gene            complement(858..2213)
                     /locus_tag="PLZ54_06715"
     CDS             complement(858..2213)
                     /locus_tag="PLZ54_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOJ66653.1"
                     /translation="MCFITENLNASNKKTTVFVIELKKDIGGTAWVYCQKAFNQAKEK
                     NADVILMHMNTYGGEVVFADSIRTRILNSPIPVYVFIDNNAASAGALIAIACDKIFMR
                     PGAAIGAATVVNTTGQQMPDKYQSYMRATMRTTAEAHGKDTIIQGNDTIFQWKRDPKI
                     AEAMVDDKVSIPGIIEAGKTLTFTAEEALQHGYCDGIAESIDEIIKNHITEANFEIIV
                     FQPSFWDKVKGFFSSSIVRSILIMLIIGGIYFELQTPGIGFPLAISILAAVLFFVPLY
                     IDGLAANWEILIFLLGLVLLALEIFVIPGFGIAGISGILFSVAGLTLSLINNVNFNFD
                     GVQAGTVGKSLLTVLVGITLGFVLILYLSNKIGSTGIFKKMALNTALENKEGYIGVSM
                     DELQLVGKTGYASTVLRPSGKVNVEGKVYDAVSTEGFIEKGVQVRVLEYRTGQIYVEK
                     I"
     gene            2498..3004
                     /locus_tag="PLZ54_06720"
     CDS             2498..3004
                     /locus_tag="PLZ54_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOJ66654.1"
                     /translation="MKPNQFENELIALQNNMRNFAFSLTLNREDAEDLLQDTTLKVLD
                     NREKFTKNVNFKGWVLTIMKNIFINNYRKIVRNQTVIDKTEDMYHLNLPQNSGFDSPE
                     GSYSIGEINSNIEAFPDEYRIPFSMHLQGYKYEEIAETMHLPIGTVKSRIFLARKRLQ
                     QQLKDYRD"
     gene            3127..3495
                     /locus_tag="PLZ54_06725"
     CDS             3127..3495
                     /locus_tag="PLZ54_06725"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOJ66655.1"
                     /translation="MNTAIVLLGSNVDANENIDAAKERLSEYFEIDLCSSRIGSEPVG
                     ENYVTPFLNEAVKLYSDETSEETIAIFKQIEIDMGRTPESKLTGLVPIDIDLIFWNDN
                     LMRNDYNRFSFVKRCVDEIK"
     gene            complement(3882..5111)
                     /locus_tag="PLZ54_06730"
     CDS             complement(3882..5111)
                     /locus_tag="PLZ54_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ66656.1"
                     /translation="MKKIILSFFLVLSFTFTYAQKSNVSKAKNKALMENPDFAGAREA
                     IQAALNDSITKNDPNTWYVAGLIGYKENDYWYQKLILNQKYDADAKGQAILESYNYFK
                     KAAELDQLPDAKGKVKPKFLKDIKTKIKEYYTLQPNLIGYGAYLYEKKDYEGALKVFE
                     TYLEIPTLPYLKDEIVIDSTYYMIQYFAALSATNAGYHDKAISLYEDLKHKNYETLNV
                     YELLADEYRTINDTIKYVETLKEGLEKFPSELWFLQNLINHYIYSGKIQDALAYLNDA
                     IKRDPNIAQYYFVKGNLEENLGNYDEAISAFNKAIELDPNLAEAYAGIGRLYYNKAIK
                     MLEVANDIKDVKLFNQEKEKANKVFKESIPYFQKAKELNPNEIEYKRTLRTLYYRLQM
                     DKEFEAIDEEIRAMENQ"
     gene            complement(5281..7818)
                     /gene="gyrA"
                     /locus_tag="PLZ54_06735"
     CDS             complement(5281..7818)
                     /gene="gyrA"
                     /locus_tag="PLZ54_06735"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOJ66657.1"
                     /translation="MIEQERIIKVNIDEEMKSSYIDYSMSVIVSRALPDVRDGFKPVH
                     RRVLFGMNELGNTPDKPYKKCARIVGEVMGKYHPHGDSSIYGTLVRLAQPWSMRYPLV
                     DGQGNFGSVDGDSPAAMRYTEARLQKIAEEMLADIDKDTVDFQPNFDETLKEPVVLPS
                     RLPNLIVNGSSGIAVGMATNMAPHNLSETVDAIVAYIDNNDISIQELTKFIKAPDFPT
                     GGIIYGYAGVKEAFETGRGRIVIRSKVEIETEASGREKIVVKEIPYQVNKVELIKTIA
                     NLVEEKKIEGISHINDESDREGMRIVIEVKRDANSNVVLNKLYKLTALQSTFNVNNIA
                     LVHGRPHMLNIKDLIHYFVEHRHDVVIRRTKFELAEAEKRAHLLEGFMIILDNLDEAI
                     QIIRNSKTPDEARSNLMSRFNLTEIQARAIVEMRLRQLTGMEQEKLRAEYQEILALIA
                     HLKEILDKIELRMQIIKDELLEIKAKYGDNRRTEIVYSSEEFNPEDFYSNDEMIITIS
                     HLGYIKRTPLSEFRTQGRGGIGSKGSDSRDEDFIEHIYPASMHNTMLFFTQKGKCYWL
                     KVYEIPEGSKSSKGRAIQNLLNIDGDDKVTAFIRVTGLTDPDYNSSHYLVFCTKKGTI
                     KKTSLEAYSHPRQNGVTAINIREDDEVIQVRLTDGNSEILMANRNGRAIRFHESTVPE
                     MGRVSTGVRGMKLDDEKDEVVGMICVNKEDNTNILVVSEQGYGKRTLLDDENGEPIYR
                     ITNRGGKGVKTMNITEKTGKLVAIKNVTDDNDLMIINKSGIAIRLKVADIPVIGRATQ
                     GVRLINLEKRNDEIASVCKVDSEPVEEIVDEEIIETELAENNEPNEN"
     gene            complement(8099..8536)
                     /locus_tag="PLZ54_06740"
     CDS             complement(8099..8536)
                     /locus_tag="PLZ54_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOJ66658.1"
                     /translation="METTNKIQKIIGNQETFTNIGLLIFRLIIGIFMLTHGLQKITNF
                     EMLKAFFPDPLGIGASTSLILIILAEVGCSLLIIFGIFTRLAAIPPMFGMAVAAFMVH
                     ANDPFSVKEIAFLYLFLYVALLFLGAGKYSVDYLLAKHLTKKK"
     gene            complement(8596..>9315)
                     /gene="ispE"
                     /locus_tag="PLZ54_06745"
     CDS             complement(8596..>9315)
                     /gene="ispE"
                     /locus_tag="PLZ54_06745"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOJ66659.1"
                     /translation="NVYDHLKIEPIPNAAENQFVVKGLSIDGKPENNLVIKAWQLLRD
                     EYKIAPVKIFLEKNIPMGAGLGGGSSDAAFMLKTLNDLFQLDIDREKLRNLAAKLGAD
                     CPFFIDNQPVFAQGKGDEFHPLKLSLKNFYLVLVKPDIHIATANAYAETVPKKPEFPL
                     QELVKKPIEEWKYFIKNDFEESIFKRYPLIAEIKQKLYETGAVYASMSGSGSAVYGIF
                     YDRPVRNGYFDNYFFSIVPLS"
BASE COUNT         2874 a         1788 c         1625 g         3028 t
ORIGIN      
        1 aagacgtgga tcggcattca aaatacccgg aacatctttc catatagtca aattttctgc
       61 attcagcaag ttggccacaa cagcagccgt ataatccgaa ccttcacgtc ccagagttgt
      121 cggatctcct ttcgtattgg ctccgataaa accctgacca atataaaccg gataattttc
      181 gaaatttgcg gcttccgtca atttttgttg cgattctttc atcttgacat ttgcttcccg
      241 aaaattgctg tcggttatca gcagtttccg catatccagc caacgatttt gaataccggt
      301 ttcatttagg aatttggaaa ctatttgcgt tgaaattatt tcaccatagg atacaatttt
      361 atcgtaccat cggtcataat cgtcaccaac cgaaagtttc agcatactgg taatctcgtc
      421 gtaaagaggc tgcaaaatag cgacaggatt ttcggtatcc gggaaaagtt ctgctaaaat
      481 ttcattgtga taattttcta tttgttgaaa ttttgccaga gcagaagcgt gatcggcctg
      541 cataaaatct tccaatacat cttcgagcgc attggttgtt tttcccatcg ccgaaacaac
      601 aatgaataaa ttatcattca cgccagaaac aatcgatgcc agatttttaa ttccggctgc
      661 cgaacgaacc gaagcaccac caaatttata tacaatcatg aaattatttg taaaattact
      721 gaataaaaat acctttctta ttcatttaaa tggactgcaa aagtaaaata atccgaaaaa
      781 ttacacaact gtttggagat actatttgaa aaattcaaac agataggcaa taatacgaaa
      841 aagaaagaga aaaatattta tatcttttca acataaattt gaccggtgcg atattcaagc
      901 acgcgaacct gaactccttt ttcaataaaa ccttccgtag aaacggcatc ataaactttt
      961 ccttcgacat ttacttttcc tgaagggcga agaaccgttg aagcataacc tgttttaccc
     1021 accagctgca attcatccat tgaaacgcct atgtaacctt ctttattttc gagcgccgta
     1081 ttgagagcca tttttttgaa aattcccgtc gaaccgattt tattgctcaa atacaatatc
     1141 aaaacaaatc ccaacgtaat gccaaccaaa acggtaagca acgattttcc aactgtgcct
     1201 gcctgaaccc catcaaaatt gaaattgaca ttgtttatta aactgagcgt taaacccgcc
     1261 acagagaaca aaattccgga aattccggct ataccgaaac cgggaattac aaatatttcc
     1321 aatgccaata gaaccagtcc cagcaaaaaa atcaaaattt cccaattggc agccaaacca
     1381 tcaatataca aaggaacaaa aaacaatacg gctgcaagaa tacttattgc caagggaaat
     1441 cctatgcccg gagtctgaag ttcaaaataa atacctccaa taattagcat aattaaaatg
     1501 cttcgtacaa tggagctgga aaaaaatcct ttcactttgt cccaaaaact cggttgaaaa
     1561 acaataatct cgaaattagc ttcagtaatg tgatttttaa taatttcatc aatactttct
     1621 gcaattccat cacaatatcc gtgttgcaaa gcttcttctg ccgtaaaagt caatgttttt
     1681 cctgcttcaa tgataccggg aatcgacacc ttatcgtcca ccattgcttc tgcaattttt
     1741 ggatcgcgct tccattgaaa aatggtatca ttaccctgaa taatggtatc tttcccgtga
     1801 gcttcggctg ttgtacgcat ggttgcccgc atgtacgact gatatttatc gggcatttgc
     1861 tgtccggtgg tatttacaac tgttgctgca ccaattgccg ctccgggacg cataaaaatt
     1921 ttgtcgcaag ctatggctat cagagctccg gcagaagcgg cattgttatc gataaaaaca
     1981 taaaccggaa tcgggctgtt caaaattcgt gttcgaatgg aatcggcaaa aaccacttcg
     2041 cctccataag tattcatgtg catcagtatg acatcggcat ttttctcttt ggcttgatta
     2101 aatgcctttt gacaataaac ccatgctgta ccacctatat cttttttcaa ttctataaca
     2161 aaaacagtcg tttttttatt cgaagcattc agattttcag ttataaaaca cattgtaatt
     2221 gttacaaaaa tgtaaaatat ataaaaattt ctcattttat agaatttttt ttgtcaaata
     2281 tacgaaacat ttaaaaatat gctgtatatt tgttgtgaaa atctagaaca agtattttcg
     2341 aaagagttct ttgttaagtt tttaatggaa gtataaattt gatgctattc gattcaaaaa
     2401 ctttttcaga aagattcgtt ttagaataaa aaattacctg ccaagttttt ttcgttggga
     2461 gaggtaaaat ttattcacac acttaaaatt aaaagttatg aagccaaatc aatttgaaaa
     2521 cgaattaatt gccttacaaa acaatatgcg gaatttcgct ttttcactca cgttgaacag
     2581 agaggatgca gaagatttat tgcaggatac caccctgaaa gtattggaca atagggaaaa
     2641 atttaccaaa aacgtcaatt tcaaaggttg ggtgctgacc ataatgaaaa atatatttat
     2701 caacaattat cgtaagattg ttcgcaatca gacagttatt gataaaacag aagacatgta
     2761 tcaccttaat ttgccgcaaa attccggatt tgacagccct gaaggctcct attccatcgg
     2821 tgaaattaac agcaacattg aagcatttcc cgacgaatac agaattccct tttccatgca
     2881 tttgcaggga tataaatatg aagaaatagc cgaaaccatg catttgccaa taggaacggt
     2941 gaaaagtcga atttttttag cacgtaagcg actgcaacaa caattgaaag attatcgcga
     3001 ctgacatctg aaatatttta taagccggaa aacatttttc cggctttttt gttttactga
     3061 agtaaaagtg gatttaatat ttttaccacc taccaaataa gcctttagtt tatttaaaaa
     3121 agttttatga ataccgccat cgttttactt ggaagcaatg ttgatgccaa tgaaaatatc
     3181 gacgccgcaa aagaaaggtt gtcggaatat tttgaaatag acttgtgcag ttcccgcatt
     3241 ggcagcgaac ctgttgggga aaactatgtt acaccatttt tgaacgaagc cgtgaaactt
     3301 tattccgatg aaacttccga agagacaata gccattttca agcaaataga aatcgatatg
     3361 ggtcgtactc ccgaaagcaa attaacagga cttgtcccga ttgatattga tttgattttc
     3421 tggaacgaca atttaatgcg caacgactac aaccgcttct cttttgtaaa aagatgtgtt
     3481 gacgaaatca agtaatcaaa ttttctcttt gtcggtttag ggaattaatc tttgcaaatt
     3541 cagaaacaga ggcggaaaac gtactttggt agccactatg gaagctaaag cgaaattcgg
     3601 aagcaatgaa ggaaatcacg aaaaaatttt atcggtaaac attcattcat gtttaaatct
     3661 actccgaaaa gttttatttt tgaaaattcg cttcgccccc gaccaaaaag aaaactcgtt
     3721 tttcagcaac ttataagaaa taaggttaaa aagtcgatga atttagtttt ttgacttttc
     3781 gaagcggact catgttttaa tttaaaaatg aataaaaaaa aaggagaaaa ccctaaataa
     3841 ttttctccta tatatagata attgtataaa aattgatttt atcattgatt ttccatagct
     3901 cttatttctt catcaatagc ttcaaattcc ttatccattt gcaaacgata atacaaggta
     3961 cgcaaagtac gtttgtattc tatttcgttg ggattcaatt cttttgcttt ctgaaaataa
     4021 ggaattgatt ctttaaagac tttatttgct ttctcttttt cctggttaaa caatttcaca
     4081 tccttaatat cattggcaac ttccaacatt ttaatggctt tgttgtaata caatcggcct
     4141 atgccagcat acgcttcagc taaattggga tctaattcga ttgctttgtt aaaagctgaa
     4201 attgcttcat cgtaattccc caaattttct tccagattcc cctttacaaa ataatattgt
     4261 gcaatgttcg gatcgcgttt gatagcatca ttaagatatg ccaaagcatc ctgtatttta
     4321 ccactataaa tataatgatt gataagattt tgtaaaaacc ataattcaga aggaaacttt
     4381 tctaaccctt ccttgagcgt ttcaacatat ttaatggtat cattaatagt tctatattca
     4441 tctgccaaca gttcatatac atttaaggtt tcataatttt tatgtttcaa atcttcgtaa
     4501 aggctgatgg ctttatcatg ataacctgcg ttagtagcgg acagggctgc aaaatattgt
     4561 atcatatagt aagtagaatc aatcacaatt tcatccttta ggtaaggcaa agttggaatt
     4621 tcaagataag tttcaaatac ctttaatgct ccttcatagt ctttcttttc ataaaggtat
     4681 gcaccatagc cgattaaatt gggctgtaag gtatagtatt ccttaatttt tgttttgata
     4741 tctttcaaaa attttggttt tactttacct tttgcatcgg ggagttgatc caattcggca
     4801 gcctttttga aataattgta cgattccaaa atggcttgtc ctttagcgtc agcatcgtat
     4861 ttttgattga gaatcaattt ctgataccaa taatcgtttt ctttatatcc aattaatccg
     4921 gcaacatacc aagtgttggg atcatttttc gttatagaat cgttcaaagc agcttgaatg
     4981 gcttcacgag ctcctgcaaa atctggattt tccatcaatg ctttgttttt cgcttttgac
     5041 acattgctct tttgagcata agtgaaggtg aaacttagca ccaaaaagaa agataaaatg
     5101 atttttttca tacttttatt attaaatttt tttacaataa aattttactc ttcctattga
     5161 tttactgcaa gttgattgac aaataaaatc aacttgcagt aaatatttat tttttaaaaa
     5221 aatatttttt tcagggcata aaattacaat gcaaattatt gttttccaaa tattttttaa
     5281 ttaattttca tttggttcgt tattttccgc taattcggtt tctattattt cttcgtcaac
     5341 aatttcttct accggttcgg aatctacttt gcaaacggaa gcaatctcat cattgcgttt
     5401 ttccaagtta atcaaacgga caccctgagt ggcacgacca ataaccggaa tatctgcaac
     5461 tttcagtcga atggcaattc cagatttgtt gataatcatt aaatcgttgt catcggtaac
     5521 attcttaatg gcaaccaatt ttcctgtttt ttcggtgata ttcatcgttt ttacgccttt
     5581 tccacctctg ttggtaatgc ggtaaattgg ttctccattt tcgtcatcca gtaaagttcg
     5641 ttttccgtaa ccttgttcag atacgaccag aatgttggtg ttgtcttcct tgttaacaca
     5701 aatcatgcca acaacttcat ctttttcatc gtcgagtttc atgccgcgaa cgccggtaga
     5761 aacacgtccc atttccggca cggttgattc atggaaccga atggcacggc cgttgcggtt
     5821 agccattaaa atttccgaat ttccatcggt caacctaact tgaataacct catcatcttc
     5881 gcgaatatta atggctgtta ctccattttg gcgaggatga gagtaggctt ccagtgaggt
     5941 ttttttaatt gttccctttt tggtgcagaa aaccaggtaa tgtgagctgt tgtagtcggg
     6001 atccgttaag ccggttacgc ggataaatgc tgtaacttta tcgtctccgt cgatattcag
     6061 aagattttga atggctcgcc cttttgagct tttgctgcct tcgggaattt catacacttt
     6121 cagccagtag catttgcctt tttgcgtgaa aaacaacatg gtgttgtgca ttgaagcagg
     6181 ataaatgtgt tcgataaaat cttcgtctct cgaatcgctg ccttttgaac cgatgccgcc
     6241 acgtccctgt gtcctgaatt cgctaagtgg agttcttttg atgtatccca aatgagaaat
     6301 agtaataatc atttcatcat tcgaatagaa atcttccgga ttgaattctt ctgaagaata
     6361 aacaatttcg gtacgacggt tatcaccata ttttgctttt atttccaaca gttcatcctt
     6421 tatgatttgc atacgaagtt ctattttatc gagaatttcc ttcaaatgag caatcaatgc
     6481 aaggatttcc tgatattcgg cacgaagttt ttcctgttcc atcccggtaa gttgacgcag
     6541 tctcatttct acaatagcgc gagcttgaat ttcggtaagg ttgaagcggc tcattaaatt
     6601 ggagcgtgct tcgtcaggag ttttagaatt gcggattatt tgaatggctt cgtccagatt
     6661 atcgaggata atcatgaatc cttcgagtag gtgtgctctt ttttcggctt cggccagttc
     6721 aaatttggtt cgacgaataa caacatcgtg ccgatgttca acaaagtagt gaatcaaatc
     6781 cttgatattc aacatgtgcg gtctgccatg aaccaaagca atgttgttga cattgaaagt
     6841 tgattgtaag gctgtcagct tgtaaagttt gttaagcacc acattggaat tggcatcgcg
     6901 tttaacttca ataacgatgc gcattccttc acggtcggat tcgtcgttga tatgtgaaat
     6961 tccttcaatc tttttttctt ctaccagatt ggcaatggtt tttatcaatt ccaccttgtt
     7021 aacctgataa ggtatttctt taaccacaat tttttcacgt ccagaggctt ctgtttctat
     7081 ttcaactttg gagcggatga cgatgcggcc tcgtccggtt tcaaaagctt cctttactcc
     7141 ggcataacca taaataattc cgccggtagg gaaatcgggt gctttgataa attttgtcaa
     7201 ttcctgaatg gaaatatcgt tgttgtcgat ataggcaact atggcatcta cggtttccga
     7261 taaattgtga ggcgccatgt tggtagccat accgacggca atccccgaag aaccgttgac
     7321 aattaagttg ggtaaacgtg agggaagaac caccggttct tttaaagttt cgtcaaaatt
     7381 tggctgaaaa tcaacagtgt ctttatcaat atcggccagc atttcttcgg caattttttg
     7441 cagacgggct tctgtgtaac gcatggcagc gggactatca ccatctaccg aaccaaaatt
     7501 tccctgacca tctaccagtg gataacgcat tgaccaaggt tgagccagtc ggaccaatgt
     7561 tccataaatc gaagaatcgc cgtgcggatg atattttccc atcacttcac ccacaattct
     7621 tgcacatttt ttataaggtt tatcgggagt gttgcccaat tcgttcattc cgaaaagcac
     7681 acgccggtgt accggcttaa aaccatcgcg aacatcaggt aaagctctgg atacaattac
     7741 cgacatggaa taatcaatgt acgaagattt catttcttcg tcaatgttca ctttaataat
     7801 tctttcttgt tcaatcatct atgcaatttt ttatttttat cgaaaaattg ctctaaatta
     7861 ctacttttta ctcaattgga caagattttt tacgattatt ttttatgtta ttgatagata
     7921 tttaattgaa aatgaggatt aaatttcatt ttggaaaatt tttgaatttt ttaacgaata
     7981 gttgttaatc agtttgctaa cgttaataaa aaagagctga ggaataaaaa agacgaaaac
     8041 accattttga aaatgtttcc gtcataatag gactgaaaga aatgaaaatg aatccgattt
     8101 attttttctt cgtcagatgt tttgcgagaa gataatctac cgaatatttc cctgcaccga
     8161 ggaataacaa tgcaacataa agaaaaaggt aaagaaacgc gatttctttg acggaaaacg
     8221 gatcgttggc atgaaccata aatgcagcta cggccatgcc aaacatggga ggaatggcag
     8281 ctaatcgggt aaaaatgcca aaaatgatta acagggaaca acctacttca gccaaaatga
     8341 ttaatatcag cgaagtagaa gctccaattc ccaaaggatc ggggaaaaaa gctttcagca
     8401 tttcaaaatt agttattttt tgcaagccat gtgtcaacat gaatatgcca attatcagcc
     8461 tgaaaatcaa taagccgata ttggtgaaag tttcctgatt accaataatt ttttgtattt
     8521 tgtttgtggt ttccatattt gaattaatta aattgtttac cagtaacaac aaacaagtta
     8581 agaaaatgtt tttctttagc tcagtggaac tatcgaaaag aaataattat cgaaatagcc
     8641 attgcggaca ggccgatcgt aaaaaatgcc gtaaacggcc gatccagaac ctgacatgga
     8701 agcataaacg gcgcctgttt cgtagagttt ttgttttatt tcggctatca gcggataacg
     8761 tttaaaaatc gattcttcga aatcgttttt gataaaatat ttccattctt caatgggttt
     8821 tttcaccagt tcctgcaagg gaaattccgg tttttttggc accgtttcgg cataagcgtt
     8881 ggcagtagcg atgtgaatat ctggttttac cagcaccaaa taaaagtttt tcaacgaaag
     8941 tttcaacgga tgaaattcat ctccttttcc ctgagcaaaa accggctgat tgtcgataaa
     9001 aaaaggacaa tcggcgccaa gtttggcagc caaatttctt aatttttctc tgtcgatatc
     9061 gagttgaaaa aggtcgttga gtgttttcag cataaatgct gcatcggaag aaccaccgcc
     9121 caagccggca cccataggaa tatttttttc cagaaaaatt tttaccggcg ctattttgta
     9181 ttcatcccgc agtagttgcc atgcttttat aaccagattg ttttccggtt tgccgtcaat
     9241 ggacaaaccc tttacaacaa attgattttc ggcagcattc ggaataggtt caattttcag
     9301 atggtcataa acatt
//
LOCUS       DAOSCH010000081         9312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4857_length_9312_cov_4.912607, whole genome shotgun sequence.
ACCESSION   DAOSCH010000081 DAOSCH010000000
VERSION     DAOSCH010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9312
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4857_length_9312_cov_4.912607"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="PLZ54_06750"
     CDS             <1..194
                     /locus_tag="PLZ54_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66660.1"
                     /translation="GAVVARWAHNPKVVRSNRASATKIITIQKFKECFYKMHSFFLLL
                     SIEIMDQFKKRFDFFIIEK"
     gene            290..1645
                     /locus_tag="PLZ54_06755"
     CDS             290..1645
                     /locus_tag="PLZ54_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ66661.1"
                     /translation="MKFFSSLLINVVSLTILLSFSQLMTAQEKNQLITELNVFDNDST
                     FKYSFIYNHDGKKSVENKYKLEKSHWIRSSQKEWNYEGENCISQTERKWDGSQWKPTY
                     TIEYRYENNLMTSESHYEYVNQFPRQISKIECFYENGRLISKKTYQLQDGTLKLQLEL
                     DYKYTTDQKIDSLFITSFSTATPLQYRSKFNYNLLGNVETQLLEKKNENGIWTKDEAT
                     RWYYNADSGQLTEQRKKRWNADFNFWENVQKTVYEYDNHYLKSEYSQYWNLMFWENAF
                     RYEYNYDTSGNLLNKILYLPIYNVWRKVITINYNNYEKGVSNVMESKYNFWGGSTDEL
                     VASDIPFLFNDEIDIRKAQRIEVGYSTFVDTITNSSNPDENHKNLVYPNPSEGIFYLD
                     TRHYKIQSFEVSNLSGKLLVVQNQPQETGIVDLTDLPRGIYIIKIQTIDNQIVQKIIK
                     K"
     gene            1722..3797
                     /locus_tag="PLZ54_06760"
     CDS             1722..3797
                     /locus_tag="PLZ54_06760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOJ66662.1"
                     /translation="MTLLSTAAAQTTENPFFQNYNTPHQTIPFHLIKNEHYLPAFQEG
                     IRQQEQEVEAIINNPQSPTFKNTIEALEYSGMLLEKVGAPFFNLLSAETNDELQEIAQ
                     QVSPLLTEHSNSIFLNDKLFKKVKIVYDKREKLKLNTEQKRLLEKTYESFVNNGANLS
                     DADKETFRQLTKELNLLTLQYGQNVLKETNQYNLLIRDKNLLAGLPEDALKTLAENAE
                     KAGKEGWLLDLKVTHYVPVMKYADNRDLRRELYLAYSSRCMKGGEYDNREIIRKIVNT
                     RLKIAQLLGYQTYADYVLTRRMAEKTENVYHLLDQLLEAYRPEALREYEDIQHFADEN
                     NAYFKVQAWDWAYYSEKLKAEKFAVSDELLRPYFQLENVKKGVFGLAERLYGIRFIKN
                     PKIPVYHPEVEAYEVTDRNGKFLAVLYTDFHPRDGKRAGAWMSEFKGQWKEGKKDSRP
                     QIVIVMNFTRPTESKPALLTFDEVTTFLHEFGHSLHGMLSDCTYPSLSGTNVYRDFVE
                     LPSQIMENWASQKEFLDQFAFHYQTGEKIPVELIQKIKDAENFNVGYSCLRQLGFGYL
                     DMAWHTLSSPFEGDVVDFEKQTVAKTQILPYVENTAISPTFNHIFSGGYAAGYYSYKW
                     AEVLDADAFSVFEKNGIFDQATAQSFMENILKKGGTEHPMILYKRFRGHEPSIDALLR
                     RNGIISTNN"
     gene            3842..5818
                     /gene="pulA"
                     /locus_tag="PLZ54_06765"
     CDS             3842..5818
                     /gene="pulA"
                     /locus_tag="PLZ54_06765"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOJ66663.1"
                     /translation="MMLVTGWFCSCQKTPKYASYDDYPVYDGSDLELTYTSEASTFRV
                     WAPTAAEVKLLLYDDGIDDGASRVVNMKRSENGTWKLRIKEDLKGKFYTFQVKVNDRW
                     LDETPGMWVKATGVNGKRAAIIDMIETNPAGWEMDVRPPLKDEADIILYEVHVRDFSM
                     SPNSGMKNKGKFLAFTEHGTKNTMGQATGIDHLKELGVTHVHLLPSFDFASIDETKLN
                     ENHYNWGYDPLNYNVPEGSYSTDPYDPAVRIKEFKQMVQALHQSGIRVVMDVVYNHTF
                     TGENSHLNLLVPGYFYRMNPDGTWSNASGCGNETASERAMMRKFIVESVVYWATEYHI
                     DGFRFDLMGVHDIETMNAVRLALDKVDPTICIYGEGWTAGPSVYPEEKRALKKNAKQL
                     DRIAVFSDDIRDALKGSWQHPEIPGFVSGVDSLEESVKFGVVGSIQHPQIDYSRLIYS
                     KEPYVNDPTQVISYVSCHDDLCLADKLRSSKPLGATNDEILRFDKLAQTIVFTSQGIP
                     FIYAGEELFRTKKGVHNSYQSPDSINQINWDNKTVFQDLYQYYKGLIALRKAHPAFRL
                     GTQENVQQYLHFLDWGIPNVVAFTLTDNAKGDTWRNILVIYNGNRKSVVVEIPEGEWN
                     LVCHDGRINLTGIAQVKTTAFVVAPSSASILYQQ"
     gene            5877..7871
                     /locus_tag="PLZ54_06770"
     CDS             5877..7871
                     /locus_tag="PLZ54_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HOJ66664.1"
                     /translation="MEEDKITGLPENAHRELKEGEEYKPILPAEKVFPEVNFRSVSTG
                     LLMAVLFSAAAAYLGLKVGQVFEAAIPIAIIAVGLSGAAKRKNALGENVIIQSIGASS
                     GVIVAGAIFTLPALYILQAKYPDISVNFMQVFLSSLLGGVLGILFLIPFRKYFVSDMH
                     GKYPFPEATATTQVLVSGEKGGDQAKPLIVAGIIGGLYDFIVATFGAWAEVFTSRVLP
                     FGAQIAEKAKLVFKVNVGAAVLGLGYIVGLKYAAIICAGSALIWFVVVPVLPLLNDQV
                     IHLLNPAFQGTLAAMSPEIIFRDFARHIGIGGIAMAGIIGVAKSWKIIMDAVGLAAKE
                     LKGGAKSLEQQKIKRTQRDISMKIIIFGIIGALLATFLFFYFGVVGNLTHAVVGILVI
                     GIIAFLFTTVAANAIAIVGTNPVSGMTLMTLILSSVIMVAVGLKGTAGMTASLIMGGV
                     VCTALSMAGGFITDLKIGYWIGTTPAKQESWKFLGTLVSAATVGGVIMILNKTYGFTG
                     ENALVAPQANAMAAVIEPLMSGVGAPWLLYGMGALIALVLNFFNVPALAFALGMFIPL
                     ELNVPLLVGGLVSWYVSGRSSNKELNRLRQEKGTLLASGFIAGGALMGVVSAALRFGG
                     IQLTNESWLQQPGSEWLALFMYVLLILYFVWDSMRIKDNK"
     gene            7916..9235
                     /gene="ffh"
                     /locus_tag="PLZ54_06775"
     CDS             7916..9235
                     /gene="ffh"
                     /locus_tag="PLZ54_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004312925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOJ66665.1"
                     /translation="MFESLSERLERSFKILKGEGRITEINVAETLKDVRKALLDADVN
                     YKTAKTFTDTVKEKALGQNVLTSIKPSQLMIKIVHDELAQLMGGSSVDINLKGSPAVI
                     LMAGLQGSGKTTFSAKLANLLKTKRGKNPLLVAGDVYRPAAIEQLKVLGQQLKIPVYS
                     EEDNKNPVSIAEAAIKYAKLHGYDVVIVDTAGRLAVDETMMNEIAAIKKAIQPQEILF
                     VVDAMTGQDAVNTAKEFNDRLNFDGVVLTKLDGDTRGGAALSIRSVVDKPIKFVGTGE
                     KMDALDVFHPERMADRILGMGDIVSLVERAQEQYNEEEARRLQKKIAKNQFDFNDFLS
                     QISQIKKMGNIKDLASMIPGMGKALKDVDIDDSAFTGIEAIIFSMTPEERENPKLLNG
                     SRKLRIAKGSGRSIVEVNRLLKQFDQVGKMMHMATVGQGKLKMKKRK"
BASE COUNT         2876 a         1687 c         2034 g         2715 t
ORIGIN      
        1 gtggagcagt ggtagctcgt tgggctcata acccaaaggt cgttcgttcg aatcgggcct
       61 ccgcaacaaa gattattacg attcaaaaat ttaaggaatg tttctataag atgcattcct
      121 tttttttgtt gctcagcatc gaaatcatgg atcaatttaa aaagagattt gattttttta
      181 ttattgagaa atagtagata agcactttaa tttaggtaaa gaaatgaatt tgttccatta
      241 aatatttatg cgtatttttg aaaaattttt ctccaatttg cgtgtattca tgaaattttt
      301 ttcttcctta cttataaatg ttgtatcgct gacaatttta ctctctttct cacagcttat
      361 gacagctcag gaaaagaatc agctaattac cgaactcaat gtgttcgata acgattccac
      421 ttttaaatac agtttcattt ataatcatga tggaaaaaaa tcggtcgaaa ataagtataa
      481 acttgaaaaa agccattgga tacgctcttc tcaaaaagaa tggaactacg aaggagaaaa
      541 ctgcatctct caaaccgaga gaaaatggga cggaagtcag tggaaaccga cttatactat
      601 cgaatacagg tatgaaaaca atttgatgac ttccgaaagt cattacgagt atgtcaatca
      661 gtttccccgg caaatcagca agatagaatg cttttatgaa aacggtcggc tgatttcaaa
      721 aaaaacttat caactccaag atgggacgtt gaaactgcaa ttggagttgg attacaaata
      781 cactactgac cagaaaatcg attctttgtt cattacttca ttttccactg caaccccact
      841 gcagtaccgt tcaaagttca attataactt acttggaaat gtagaaaccc agttgttgga
      901 gaaaaaaaat gaaaacggaa tttggacaaa ggacgaggca accagatggt attacaatgc
      961 agattcggga caattgacag agcagcgaaa aaaacgatgg aacgcagatt tcaacttttg
     1021 ggaaaacgta caaaaaaccg tttacgaata cgataatcat tatttgaaat cggagtacag
     1081 tcagtattgg aatttaatgt tttgggaaaa tgcctttcgc tacgaataca attatgatac
     1141 ttccggcaat ctacttaaca aaatactgta tttacccatt tacaatgtgt ggcgaaaagt
     1201 tattaccatc aattataaca attacgaaaa aggcgtttcc aacgttatgg aatcgaaata
     1261 taatttttgg gggggatcta cagatgaact ggttgcttct gatattccat tcttgttcaa
     1321 cgacgaaatt gacatcagaa aagcacaacg gatagaagta ggatattcaa cctttgtcga
     1381 taccattacg aatagttcaa atcctgacga aaaccataaa aatctcgttt atcccaatcc
     1441 atcggaaggc attttttatc ttgacacccg gcattataaa attcaatcct ttgaagtcag
     1501 caatttaagt gggaagctgt tggtggttca aaatcagcca caagagacag gtattgtcga
     1561 tttgaccgat ttacccaggg gaatatatat tataaaaata caaactattg acaatcagat
     1621 tgttcaaaaa attataaaaa aatagattac catttacaaa aatatatatt taacagttta
     1681 attgttaatt gagcatgaac ataaaagtta cattactaat gatgacactt ctttctaccg
     1741 cagcagcaca aactacagaa aatccttttt ttcagaatta caacactcca caccaaacca
     1801 ttccttttca tctgataaag aacgaacatt atttgcccgc ttttcaggaa ggaatccgtc
     1861 aacaagagca agaagtggag gccattatca ataatcctca gtcgccaaca ttcaaaaaca
     1921 ccatagaagc cctcgaatat tccggtatgt tactggaaaa agtcggcgca ccttttttta
     1981 accttttgag tgcagaaacc aatgacgaac ttcaggaaat tgcacaacag gtttctcctt
     2041 tactgaccga acattccaac tccattttct taaacgacaa gttattcaaa aaggtcaaaa
     2101 tagtttacga taaaagagaa aaacttaaat taaacaccga acaaaaaagg ttattggaga
     2161 aaacgtacga aagttttgtg aacaacgggg ccaacctttc cgatgccgat aaagaaactt
     2221 tccgtcaact gaccaaagag ctgaatttgc ttactttgca atatggacag aatgtactga
     2281 aagaaacaaa tcagtacaat ttattgatta gggataaaaa tttgttggcc ggattgcctg
     2341 aagatgcatt gaaaactttg gcagaaaatg ccgaaaaagc agggaaagaa ggatggttac
     2401 tcgacctgaa ggtaacgcat tatgttcccg ttatgaaata tgccgataac cgtgatcttc
     2461 gacgagaact ttatcttgcc tattcttccc gatgcatgaa aggtggagaa tacgataatc
     2521 gggaaatcat ccgtaaaatc gtcaatacgc gtttaaaaat cgcccaactc ttgggatacc
     2581 aaacttatgc cgactatgtg ctgaccagaa gaatggcaga aaaaaccgaa aatgtttatc
     2641 atttgctcga tcagttgctg gaagcttatc gtccggaagc gcttagagaa tatgaagaca
     2701 ttcagcattt tgccgatgag aataatgctt attttaaggt tcaggcttgg gattgggctt
     2761 attattccga aaaattaaag gccgaaaaat tcgcagtgag cgacgagttg cttcgaccct
     2821 attttcaact tgagaacgta aaaaaaggtg tttttggtct ggccgaacgc ttgtatggaa
     2881 ttcgttttat caaaaaccct aaaattcctg tttatcatcc agaagtagaa gcttatgaag
     2941 ttaccgacag aaatggtaaa ttccttgctg tactttatac cgattttcat ccacgtgatg
     3001 gcaaaagagc cggcgcttgg atgagcgaat ttaaaggcca gtggaaagaa gggaaaaaag
     3061 acagtcgtcc gcaaattgtg attgtgatga atttcactcg accgactgaa tctaaacctg
     3121 ccttgctgac ttttgatgaa gttacaacct ttttgcacga atttggacat tcgctacatg
     3181 gaatgctttc cgattgtact tacccatcgc tttcgggaac aaatgtttat cgcgattttg
     3241 tggaattgcc ctcacaaatc atggaaaatt gggcttccca aaaggaattt ctggatcagt
     3301 ttgcttttca ctaccagacc ggtgaaaaaa ttccggttga gttgattcaa aaaataaagg
     3361 atgccgaaaa ttttaatgtc ggctattcct gcttgcgtca acttggtttt ggatatttgg
     3421 atatggcttg gcatacactt tcttcacctt ttgaaggaga cgtggtagac tttgaaaaac
     3481 agactgtggc caaaacccaa attttgcctt acgtggaaaa tacggcgatc agtcctactt
     3541 tcaatcacat attttcgggc gggtatgccg ccggatatta cagttacaaa tgggctgaag
     3601 tactcgatgc cgatgctttt tctgtttttg aaaaaaatgg cattttcgat caggctacag
     3661 ctcaatcttt tatggaaaat atcttaaaga aaggtggtac cgagcatccc atgattttat
     3721 acaaacgttt tcgcggacat gaaccttcta tcgatgcttt actcagaaga aacggaataa
     3781 ttagtacaaa taattgattt taaataaagt ataacgaaca atgaaaaaac atttgatatt
     3841 tatgatgttg gtaaccggat ggttttgctc ttgtcaaaaa actccaaaat atgcttctta
     3901 cgacgattat ccggtctatg atggttctga ccttgaactg acttatactt ccgaagcttc
     3961 cacgttcagg gtatgggctc ctacggctgc ggaagtcaag cttttgcttt atgatgatgg
     4021 cattgatgac ggagcatcgc gagtagtaaa catgaagcgc agtgaaaatg gaacatggaa
     4081 attgcggata aaagaagatt tgaaaggtaa attttacact ttccaggtaa aagttaatga
     4141 tcgatggttg gatgaaactc ccggtatgtg ggtaaaagca accggtgtta acggcaaaag
     4201 agctgccatt atcgatatga tagaaactaa tcctgccggc tgggaaatgg atgtacgacc
     4261 acccttgaaa gacgaagccg atatcatact gtatgaggta catgttcgtg atttttccat
     4321 gtcccccaat tcgggaatga aaaataaagg taaatttctt gcttttaccg aacatgggac
     4381 aaaaaatacg atgggtcagg ctaccggaat agaccattta aaagaacttg gagttactca
     4441 tgttcattta ttgcccagct ttgattttgc atccattgat gagacaaaac ttaatgaaaa
     4501 tcattataat tggggatacg atccattaaa ttacaatgta cccgaaggaa gttattccac
     4561 cgatccttat gatccggctg tgcgaattaa agaattcaag caaatggttc aagctttgca
     4621 tcaatcagga attcgcgtgg taatggatgt ggtttacaac catactttta ccggagaaaa
     4681 ttcgcatctc aatttgctgg ttccgggata tttttatcgc atgaaccctg acggaacatg
     4741 gtcaaatgca tccggatgtg gaaatgaaac ggcttccgaa cgtgccatga tgcggaaatt
     4801 tatcgtcgaa tcagtcgttt attgggctac cgaataccat atcgatgggt tcaggttcga
     4861 tttaatgggc gttcacgata ttgaaaccat gaatgccgta cggttggcac tcgacaaagt
     4921 agatcctact atctgtattt atggagaagg ttggacagct ggtccatcgg tatatccaga
     4981 agaaaaaaga gctctgaaaa aaaatgccaa gcaattggat agaattgccg tttttagcga
     5041 cgacatacgc gatgcactca aaggtagctg gcagcatccc gaaattcccg gctttgtttc
     5101 gggagtggac agtttggagg aaagcgtaaa atttggagta gtcggttcca ttcagcatcc
     5161 ccaaattgat tattccagac tgatttattc taaagagccg tatgtaaatg atcctaccca
     5221 ggtaatcagc tatgtttcct gccacgacga tttgtgcttg gccgacaagt tgcgttcctc
     5281 caaaccttta ggagcaacca atgatgaaat attgcgattc gacaaactgg ctcaaaccat
     5341 tgtttttact tctcaaggca ttccttttat ttatgccgga gaagaattgt tccgaacaaa
     5401 aaagggggtt cacaattctt atcagtcgcc cgattccatc aaccaaatca attgggacaa
     5461 caagaccgtt tttcaggatt tgtatcaata ctataaagga ttaattgctc tgcgcaaagc
     5521 tcatcctgct ttcagacttg gcacacaaga gaatgttcaa caatatttgc attttttgga
     5581 ttggggaatt cccaatgtag ttgcgtttac gcttaccgat aatgccaaag gagatacttg
     5641 gcgcaatatt ctggttattt acaatggcaa tcgaaaatct gttgtggtcg aaattcccga
     5701 aggagaatgg aatttggttt gccacgacgg acggattaat ttgaccggca ttgctcaggt
     5761 gaaaacaacc gcttttgttg tggctccttc ttctgcaagt attttgtatc agcagtaaaa
     5821 ataacagaat tgagaattaa tttcagttga aaaaacgaac gtaacgaaat cataaaatgg
     5881 aagaggacaa aattacagga ttaccggaaa atgcacaccg ggaattgaaa gaaggggaag
     5941 aatacaaacc gattctccct gcggaaaagg tttttccgga agtaaatttt cgctctgttt
     6001 cgaccggatt actaatggct gttctttttt cggcagctgc agcctatctg ggtttaaaag
     6061 tcggacaggt ttttgaagca gctattccca tagccattat cgcagttgga ctttcgggag
     6121 ctgcaaagcg aaaaaatgct ttgggagaaa acgtcattat ccagtccatt ggggcaagtt
     6181 cgggcgttat tgtggcaggc gctattttta ctttacccgc tttatatatt ctgcaagcca
     6241 aatatcccga tatttcggtg aattttatgc aggtttttct cagttcgcta ttgggtggcg
     6301 tattgggtat cctctttctg ataccatttc gtaaatactt tgtttcggat atgcacggca
     6361 agtatccttt tccggaagca acagctacta ctcaggtact tgtttcgggc gaaaagggtg
     6421 gcgatcaggc caaacctttg attgtagccg gcataattgg tggcttgtat gattttattg
     6481 tcgcaacttt tggcgcttgg gctgaagttt ttacttcgcg ggttttgcct tttggcgcgc
     6541 aaattgccga aaaagccaaa ctcgttttca aagttaatgt cggagctgcc gtattgggat
     6601 tgggatatat cgtcggatta aaatatgctg ccattatttg tgcgggttca gctctgattt
     6661 ggtttgtagt tgttcctgta ttgcctcttt tgaacgatca ggtaattcac ctgctgaatc
     6721 cggcgtttca gggaacttta gctgccatga gtcctgaaat tatttttaga gattttgctc
     6781 gacatatcgg tattggagga attgcgatgg cagggattat cggcgtggca aaatcatgga
     6841 aaattattat ggatgcggtt ggtttggccg caaaagaact gaaaggcgga gcaaaatcac
     6901 tcgagcagca aaaaatcaaa cgtacccagc gcgatatttc aatgaaaatt attatctttg
     6961 gaataatcgg agcattgttg gctacttttc tgttttttta ttttggtgta gttggcaatt
     7021 tgacacatgc tgtggttggg atactggtga taggtattat tgcctttctg tttactacgg
     7081 tagctgccaa tgccattgct attgtgggta ccaatcccgt ttcgggcatg acgttgatga
     7141 cgctgattct ttcttccgtg attatggtag cagtgggctt aaaaggaacg gcaggtatga
     7201 cggcatcatt gattatggga ggtgtggttt gcacagccct gtctatggca ggaggcttta
     7261 tcaccgattt gaaaattggt tattggattg gaacaacgcc ggccaaacag gaaagctgga
     7321 aatttttggg aacgcttgtt tcggctgcta cagtgggtgg tgttattatg attctgaaca
     7381 aaacttacgg atttacaggt gaaaatgctt tggttgcacc tcaagccaat gcgatggcag
     7441 cggtaattga gccgctgatg tcgggagttg gtgcgccgtg gctcttgtat ggcatgggag
     7501 ccctgattgc tttggtactg aattttttca atgttcctgc gctggctttt gcactcggaa
     7561 tgtttattcc gctggaatta aatgttccct tgctggttgg tggacttgtt agctggtatg
     7621 tttcgggccg aagcagcaat aaggaattaa accgtttgcg tcaggaaaaa ggaacattgc
     7681 ttgcttcggg atttatagcg ggtggtgcat taatgggtgt agtcagcgct gccttgcgtt
     7741 ttggcggtat tcaactcacc aatgaaagtt ggctgcaaca gcccggttcg gaatggttgg
     7801 cactctttat gtatgtattg ctcattttgt attttgtttg ggacagcatg cgaataaagg
     7861 acaataaata aaaatttaag gaataaaatt aatttataaa cgaggataat taaaaatgtt
     7921 tgaatcactt tctgaaagac ttgagagatc ttttaaaatc ttaaagggtg aaggacggat
     7981 aaccgaaatc aatgtggcag aaacattgaa agacgttaga aaagctttgc ttgatgccga
     8041 tgttaattat aagacagcta aaacctttac cgatacggta aaggaaaaag cattgggtca
     8101 aaatgtattg acttcgatta agcccagtca gttgatgata aaaattgttc acgatgaact
     8161 ggctcaactt atgggcggct caagtgtgga cattaatctg aaaggttcgc cggctgtaat
     8221 cctgatggcc ggtttgcaag gttcaggtaa aactactttt tcggccaaac ttgccaatct
     8281 gttgaaaaca aaacgaggaa aaaatccctt gttggttgcc ggcgacgtat atcgacctgc
     8341 agctatcgag caattgaaag ttttgggtca gcagcttaaa attcctgtgt attctgaaga
     8401 agacaataaa aatcctgttt caattgccga agcagccata aaatatgcca aacttcacgg
     8461 ttatgatgtg gtcattgtgg atactgctgg gcgtttggca gttgatgaga cgatgatgaa
     8521 cgaaatagca gccataaaaa aagccattca gcctcaggaa attttatttg tcgtggatgc
     8581 catgacgggt caggatgctg tgaatacggc aaaggaattc aacgatcgac ttaattttga
     8641 tggtgtggtg ctgaccaaat tggatggcga tacacgtggc ggagctgcac tttccattcg
     8701 ctcggttgtc gacaagccca ttaaatttgt tggaactggt gaaaagatgg atgctttgga
     8761 tgtgttccat cccgaacgaa tggcagaccg tattttgggt atgggagata ttgtatcgtt
     8821 ggtagaaaga gctcaggaac agtataatga agaagaagcc cgaagattac agaaaaaaat
     8881 tgccaaaaat caatttgatt ttaacgattt tctgtcccaa atttcccaaa tcaaaaagat
     8941 gggaaacata aaggatttgg cttccatgat tcccggcatg ggaaaagcct tgaaagatgt
     9001 ggacattgac gatagcgcat ttaccggtat tgaagccata attttctcca tgacacctga
     9061 agaacgcgaa aacccgaaat tgctgaacgg aagcagaaaa ttgcgcattg ccaaaggtag
     9121 tggaagaagt attgtggaag taaaccgatt gttgaaacaa ttcgaccaag taggtaaaat
     9181 gatgcacatg gcaacagtag ggcagggaaa attgaaaatg aagaaaagaa aataacgatt
     9241 atagtttaca ttttgttgat tttatcagtt aaactcaaac ttgcacctaa accgtgttgt
     9301 ctgttttaat tt
//
LOCUS       DAOSCH010000082         9253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_4895_length_9253_cov_5.871168, whole genome shotgun sequence.
ACCESSION   DAOSCH010000082 DAOSCH010000000
VERSION     DAOSCH010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4895_length_9253_cov_5.871168"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1466)
                     /locus_tag="PLZ54_06780"
     CDS             complement(105..1466)
                     /locus_tag="PLZ54_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOJ66666.1"
                     /translation="MAKKERINFSKIGAIAAATGSAVGLGNIWRFPYLLGESGGGAFL
                     LVYILCVVFLGLPVMISEFSIGRMAQANAAGSFRTLAPKSKWWLVGLMGVISAFLILG
                     FYVVVSGWTLEYIVQALSNKFANASTAQLNQVFTDFSTNTYRPVGWMLLFTALTCFII
                     IAGVKNGIEKSTKLMMPLLFLIIIILGIRSITLPNGTEGLKFLFKADFGKIDSEVILN
                     AMGQAFFSLSLGMGCMITYGSYINKDNNLSSTALQVTIFDTLVAVLASIAIFPAVFSF
                     GINPAQGPELVFITLPHIFSQMQGSTIWSTLFFILLALASLTSTISLLEVIVAFLTEE
                     LHISRAKAAIVSSLGIALLGILSSFSLGIWKDFSLFGYGFFALFDSITSKILMPVGGI
                     FISLFVGWRIDKQKILLELTSNGKYKIPFLNIFLLITRYLAPLAILLVLLNQLGLTKW
                     LGF"
     gene            1623..2201
                     /locus_tag="PLZ54_06785"
     CDS             1623..2201
                     /locus_tag="PLZ54_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005681997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP diphosphatase"
                     /protein_id="HOJ66667.1"
                     /translation="MKKLVFATNNLHKLQEVRAILDGKVQIQSLSEIGCLDEIPETAD
                     TLEGNALLKAKFVYDRFQLDCFADDTGLEIEALGGEPGVFSARYAGLEHDSYKNRMKV
                     LRLMEQQTNRKAQFRTVIALVLQGKEYLFEGKVEGVITREPRGEGGFGYDSIFVPNGY
                     EQTFAELSAEQKNQISHRAHAVKKFADFIVSR"
     gene            2414..4756
                     /locus_tag="PLZ54_06790"
     CDS             2414..4756
                     /locus_tag="PLZ54_06790"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ66668.1"
                     /translation="MKNKIFYISVFCSFFACFVQADNIGMGKWRMHLAYNSVGQIAQS
                     DKKIYAVSDGALFSVDKIDGSMEFYSKISGLHDTSVSRIEYDQNTRQLLVIYANGNID
                     FINEGGVINLPDLYNKQMTFSKDVQHITFFGNRAYLSCHFGIVALNMVKKEIAETYFI
                     GADAAAVDVLCTTVHGQTIYALSKSAVYKADVTNPYLVNYEVWSTVANLPGSGNFQSV
                     VSFANQLFLLRNGKIYQMNAAGQWSPFLSQVTASNLLVSDGSLFILSTNNNIYLLDNQ
                     LVSKTISGLGAVADAEYDSENNTCWFAANGNGIISYRQEGSLLPVVSTYKPSGPAVNI
                     PWQMRFSGQKLFVVPGGRSSAQYNQQGYVMMFENGVWTNIDGKTIETQTGKRVLDFMD
                     IAVDPNDPTHFLVPSYGTGIYEFKNNQFSKWYNFTNSLIETIFPNDPNGSYLYMRMDG
                     GVFDSEGNVWFTNTRASYGIKILKPDGQWTQLAYAGITSRPTLGQILIPSLNPNQKWI
                     LSVRSEPGICVIDDNGTIDDQRDDKSVFFPYFRNTDEAASNIAPTLYNCMVEDKNHVI
                     WVGTDMGPLLFYNPSNAFDADFTCSRVKIPRNDGTNLADYLLADETIQAIAVDGANRK
                     WIGTKSSGVYLMSENGQETLQHFTVSNSPLLSNNIMSIAINPVSGEVYFGTGKGIVSY
                     QSDAAEAGSSFGDVHAYPNPVRETYNGVITITGLMENTHIKITDLNGNLVYETTSNGS
                     LATWNGKNLHGQKVSTGIYLVIGASKDGTQSTITKILVIN"
     gene            4765..5784
                     /locus_tag="PLZ54_06795"
     CDS             4765..5784
                     /locus_tag="PLZ54_06795"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOJ66669.1"
                     /translation="MKGRVKWVDIAKGIAVSLVVYGHFGTSEAAKSVIYAFHIPLFFF
                     LSGLFFSFDKFPSYKLFLKKRSVQLLIPYLFFNFITYLLWLFIGRKFGSDISSNIHPL
                     KPILGILYGNASNLPHNVPLWFLTCLFSVENLYFFIFRKTSKQQHFFLLLIIVAIAFI
                     NYKLNHVFRLPWGIDIALSMLLFYGLGVNLKSEFLEKNRNKASLSGLLIISFFVVLII
                     ARLNGKIEVSDNYFGNYIYFVLGALAGIIFIVSLSMLIAQVKLKYSLLSFLGRNSLII
                     FGLHLYAGSFVKTISAFVFHLPLSIYETPTVSLLLTIFSIVVLLPVVFFINKYLPFVV
                     GKSRK"
     gene            5784..7319
                     /locus_tag="PLZ54_06800"
     CDS             5784..7319
                     /locus_tag="PLZ54_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HOJ66670.1"
                     /translation="MKSVIKSSLPFFLFVLSCTVWAGIFFILPYFLDNPSKGWEGRFI
                     TFFHWGLECFATFLLIYALAVNKFVFAVFFPLFSLLGSILSFYRYAFKAVLTPMLIDA
                     SLHNDLRTTLDVVSIPLVIFVIISVSISILAVVYRFKKIKVTKPLIHFAVSVLLLCIL
                     LTANGRVTNSLMQRFPFNVYYNFSEYKKLRQTLVAERIDPDPALKATCSDSMKVVIVI
                     GEALRADHLSLNGYHRLTNPRLSKRENIFSFSNIYSEFTNTNMSLPQILTRADSIYPE
                     RAFQEKSFISLFKKCGYYTSWISNQDPAETYVSFINECDTVLYGHPEKSVYNYNEWLD
                     EDLFPLVKEIKKKDFPCNLFVLHTIGSHWYYNNHFSSSFKVFTPVTSSRILSQCSSEE
                     LINSYDNTVVYTDFFLDSLISIFENENAVVIYLSDHGESLGENGTWLHAGDSEPSHRP
                     ACIVWCSEKFEKKYPDKVEALRRNKNKYYRSNFVFHSALDAANIQSKVMDKKLSIFNL
                     PAQ"
     gene            7319..8476
                     /locus_tag="PLZ54_06805"
     CDS             7319..8476
                     /locus_tag="PLZ54_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66671.1"
                     /translation="MEKELIHKQSSLSFFLLNKYFWAACVVLGLIVKAYFLPVKTGDY
                     VVYLKPWIDFIKTHGYFSALKYDFSNYTPAYLYFLTLVAKIPIEPVFAIKIFSIAFEF
                     LAAYFLGKIVCLKYHNSLIILISLAVIPWLPTVMLNSSYLSQCDAIYASFVMGSIFYA
                     LKKRPFLSVLFLGIAFAFKMQAVFVLPFFFVFLLRKEIKWYYFFIVPAVYLVSILPAW
                     FNGRSFVELISLYFSQADYYRLLTMNFPNIYIWFSGADFDTFSLAGILFTVLLTLVAG
                     FWLRTPKITFTLETWIKFLFLSFIVVPFVLPGMHERYMYLGDIFGLAYLMFFPKKWYL
                     PFGILLVSTYSYIRCSRFSEVLPMQPAFFLYLLVILLTFYDFVESLKFSRK"
     gene            8473..>9253
                     /locus_tag="PLZ54_06810"
     CDS             8473..>9253
                     /locus_tag="PLZ54_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66672.1"
                     /translation="MNAVHKVNFTFLGVIIFVYVLLTAGCLNNCYFWDVVQQVSKEAH
                     WYYQTNFKYLIIPSQNSLSDIVATGYHPPLMGMLTAALWKIFGVHLWISHLLTLICFF
                     VLLYQTLKLVEKFFPAHQAGWVMLVVFMEASLLAQFSIASPDFILLTAFIASLRGLLE
                     RNTWLSSIALIFLFGINMRGIFAGIALWISFQLFLYLNSEKKFSFTLLAASIRPFLPA
                     IILSLIYFIFYFRMNGWFSGVSEEGGHYSLPENISMIGKHFF"
BASE COUNT         2650 a         1585 c         1830 g         3188 t
ORIGIN      
        1 tgaatattgc tggcttcaat cattttttcg tgcgttttga tgctcacacg gtcattttca
       61 aatgtattca ttgttatttt tattcggaat aatgtctcaa ttttttagaa ccccaaccat
      121 ttggtcaatc ctaactgatt cagtagaacg agtagaatgg caagcggtgc aaggtatcgg
      181 gttatcaaca aaaaaatatt cagaaaaggt atcttgtatt ttccgttgct ggtgagttcg
      241 agtaaaattt tctgcttgtc gattctccag cctacaaata atgaaatgaa aattcctccc
      301 accggcatga gaattttgga agtaatactg tcgaacagag caaagaaacc atatccaaac
      361 aatgaaaaat ctttccatat ccctaatgaa aaagatgaaa gtatgcccaa taatgcaatg
      421 cctaaggaag aaacaattgc agctttcgct cgtgatatat gtaattcttc tgtgagaaaa
      481 gcaacaatta cttccagcag cgagatggtg gaagttaaag aggctaaagc cagtagaatg
      541 aaaaacaaag ttgaccaaat tgtactgcct tgcatttgag aaaaaatatg aggcagcgtt
      601 ataaatacca attcggggcc ttgcgctgga ttgataccaa aagaaaaaac tgccgggaaa
      661 atagcaatgg aagcaaggac ggctaccagc gtgtcgaaaa tggtaacttg aagggctgta
      721 gaactaaggt tattgtcttt atttatgtaa gaaccgtaag taatcatgca acccattccc
      781 aaactcaaag aaaagaaagc ctgtcccatt gcatttaaaa tgacttcgga atcgattttt
      841 ccgaaatcgg ccttaaaaag aaatttcaat ccttcggtac cgtttggtaa agtaatggaa
      901 cggattccca aaatgatgat aatcagaaac agcaatggca tcatcaattt ggtgcttttt
      961 tcaattccgt tttttacacc ggcaatgata ataaaacacg ttaatgcagt gaaaagcagc
     1021 atccatccca ctgggcggta agtatttgta ctgaaatcgg taaatacctg attaagttgg
     1081 gcagtggatg cattggcaaa tttgttgctc aatgcctgta caatatactc aagagtccat
     1141 cccgaaacga caacataaaa acccaaaatc aaaaaggccg aaatcacgcc cataagtccc
     1201 accagccacc atttgctttt tggcgccaat gtgcgaaacg aaccggcagc atttgcctgc
     1261 gccattcggc caatggaaaa ctcggaaatc ataaccggca gtcccagaaa aacaacacaa
     1321 agaatgtaca ccaacaggaa agctccacct ccggattctc caagcagata aggaaaacgc
     1381 caaatgtttc ccaatcctac ggcagagcct gtggcagccg ctatggcgcc aattttgcta
     1441 aaattgattc tctccttttt tgccatattt ttcttgtttt cgatgcatat ttgttgcaaa
     1501 agtagagaaa aaaaataaaa atcgaaatga atgggagaaa gctgacttga taattatttt
     1561 tgtaaaatag ttatctttgc atgagtttga aaattcaaaa gaatatcaac atttatttta
     1621 aagtgaaaaa attagttttt gctacaaata atcttcataa attgcaagaa gttagagcaa
     1681 ttttggatgg gaaagtacaa atacaaagtt tatcggaaat aggttgcttg gacgaaatac
     1741 cagaaaccgc cgatacgctt gaaggtaatg ctttgttgaa agcaaaattt gtttacgaca
     1801 gatttcagtt ggattgtttt gcagatgata caggcttgga aattgaagca ttgggaggcg
     1861 aacccggcgt tttttcggca aggtatgcag ggttggagca cgattcttac aaaaaccgaa
     1921 tgaaagtact gcgtttgatg gaacaacaaa ccaatcgcaa agctcaattc agaactgtta
     1981 ttgcccttgt tttacagggg aaggaatatc tttttgaagg aaaagtagaa ggtgtaatta
     2041 ctcgagaacc gagaggtgaa ggtggttttg gatacgattc catattcgtt cccaatggct
     2101 atgaacagac ttttgccgag ttgtcggcag agcaaaagaa tcaaattagc catcgagccc
     2161 atgccgtgaa aaaatttgcc gactttattg tttcaaggtg aaagtaaaac caaagttttt
     2221 cgttggtttc atttttcatc aatcattgac aaaacaaaat aaatcaggaa gttttcagtt
     2281 taattttatg gattaatgtt ttacattcaa caactttttt gaggataaat aaatttttga
     2341 aaaataaaga tttccaatac agcgtaattt agtaatttaa tacgctattt cgttaaaatc
     2401 aaatatttaa cttatgaaaa ataagatttt ttacatttcg gtcttttgta gtttttttgc
     2461 ttgtttcgtt caggcagaca atattggcat gggaaaatgg agaatgcatt tggcttacaa
     2521 cagtgtcggt cagattgcgc aatccgataa aaaaatatat gcagtaagtg atggagcttt
     2581 attttccgta gataaaatcg atggcagtat ggaattttac agtaaaatca gcggattgca
     2641 cgatacttct gtcagccgaa tcgaatatga tcaaaatact cgacaactgc ttgtaattta
     2701 tgccaatgga aatatagatt ttattaatga aggcggcgtt attaatcttc ccgatttata
     2761 taataagcaa atgactttca gcaaagacgt tcagcatatt acttttttcg gaaatcgggc
     2821 ttatttgtcc tgtcattttg gtattgtagc attgaatatg gtgaaaaaag aaattgccga
     2881 aacctatttt atcggtgccg atgcagcagc tgtggatgtg ctttgtacga cagttcacgg
     2941 gcagactatt tatgcgcttt ccaaaagtgc ggtttataag gcagatgtta ccaatccgta
     3001 tttggttaat tatgaagttt ggtcgactgt tgcaaatttg ccgggaagtg gtaactttca
     3061 atctgtggta tcttttgcca atcagctttt tttgcttcgt aacggaaaga tttatcaaat
     3121 gaatgcggcg ggacaatggt ctccttttct ttcacaggtt acagcatcaa atttgctggt
     3181 ttctgatgga agtctgttta ttttgagtac caataataat atttatttgc ttgacaacca
     3241 gttggtttca aaaactattt ctggacttgg tgcggttgcg gacgcagaat acgacagtga
     3301 aaacaatacc tgttggtttg cggccaatgg aaatggaatt atttcctatc gtcaggaagg
     3361 gtcgctgttg ccggttgtat ctacttataa gccttccggc ccggccgtga atataccctg
     3421 gcaaatgcga ttttccggac agaaattgtt tgtagtgccg ggtggaagat cttcggctca
     3481 atataatcag cagggttacg tcatgatgtt tgaaaatggt gtttggacaa atattgacgg
     3541 aaaaaccatt gaaacccaaa caggaaaaag ggtgttggat tttatggaca ttgcggttga
     3601 tccgaatgat cctactcatt ttttggttcc ttcttatggc acgggtattt atgaatttaa
     3661 aaataatcag ttcagtaaat ggtataattt cactaacagc ctcatcgaaa ctatttttcc
     3721 gaatgatccg aatggttctt atctttacat gcgaatggat ggaggtgttt ttgattcgga
     3781 agggaatgtg tggtttacca ataccagagc ttcatacggt attaaaattt tgaaacccga
     3841 cggacagtgg acgcagcttg cctatgctgg aattacttca cgtcctacct taggtcaaat
     3901 tttgattcct tcattgaacc cgaatcagaa atggattttg tccgttcgtt ccgaacccgg
     3961 gatctgtgta attgatgata atggtaccat tgatgatcaa agagatgata aatcggtatt
     4021 tttcccctat ttccgaaata cggatgaagc tgcaagtaat attgcaccga cactttacaa
     4081 ttgtatggtg gaggataaaa atcatgtgat ttgggtagga acagatatgg ggcccttgct
     4141 gttttataat ccttccaatg cttttgatgc cgattttacc tgttcgcgcg ttaagattcc
     4201 gagaaatgat ggcaccaact tggcagatta tttgctggcc gacgaaacta ttcaggctat
     4261 tgctgtagat ggtgccaacc gaaaatggat cggcacaaaa tcatccggcg tatatttaat
     4321 gtcggagaac ggacaggaaa cccttcaaca ttttacagtt tccaacagtc cgttgctttc
     4381 caacaatatt atgtctattg ccataaatcc tgtaagtggg gaggtctatt ttggaacagg
     4441 gaaaggaatc gtttcttatc aaagcgatgc agcggaagcc ggaagcagtt ttggcgatgt
     4501 acatgcctat cccaatccgg taagagaaac ttacaatgga gtcatcacca tcacgggatt
     4561 aatggagaat acccatataa aaattaccga tttgaatggg aatctggttt acgaaacgac
     4621 ctccaacggt agtttggcta catggaacgg aaaaaatctt catggacaga aagtcagtac
     4681 agggatttac ttggtgattg gggccagtaa agatggtacc caaagtacca ttacaaaaat
     4741 attggttatc aattgaattt tttcatgaaa ggaagggtta agtgggtaga tatcgctaaa
     4801 ggaatagctg tttctttggt cgtttatgga cattttggaa cttctgaagc agcaaaaagt
     4861 gtaatatatg cttttcatat accacttttc tttttcttgt ccggtttatt ttttagtttt
     4921 gataaatttc caagttataa attatttctt aaaaagcgtt cggttcaact gctaattcct
     4981 tatctctttt tcaattttat aacttattta ttgtggcttt tcattgggag aaaatttgga
     5041 agtgatattt cgtcgaatat tcatcctttg aaaccaattc ttggaatatt atatggaaat
     5101 gcttccaatt taccgcataa tgttcctttg tggtttttga cctgtttgtt ttcggtagaa
     5161 aatttgtatt tttttatctt cagaaaaaca agtaaacaac agcatttttt tttattgctg
     5221 attatagttg caattgcttt tatcaattac aaactcaatc atgtctttcg attaccttgg
     5281 ggaattgata ttgcgctgag tatgttgttg ttttatggtt tgggagttaa cctgaaatct
     5341 gagtttttgg agaaaaacag gaataaagct tcattatcgg gacttttgat aatttcattt
     5401 tttgtggtgt taataattgc tcgactaaac ggaaaaattg aagtatcgga taattatttt
     5461 ggaaactata tatattttgt gcttggagca ttggcaggaa taatttttat tgtttctttg
     5521 tcgatgctta tagctcaagt taaactaaaa tattcattac taagttttct gggccgaaat
     5581 tccttgatta ttttcggttt gcatttatat gcaggaagtt ttgtcaaaac aatttcggcc
     5641 tttgtttttc atttaccttt atcaatttac gaaacaccta ccgtctcttt attattaact
     5701 attttcagta ttgtggtatt acttcctgtt gtttttttta ttaataaata tttgccattt
     5761 gttgtgggta aatcaaggaa ataatgaaat cggttataaa atcttctctt ccttttttct
     5821 tgtttgtttt gtcgtgcact gtatgggctg gcattttttt tattttgccc tattttctcg
     5881 acaacccatc gaaaggttgg gaaggccggt tcataacttt ttttcattgg gggttggaat
     5941 gttttgccac ttttttgctg atttatgcat tagctgtaaa taaatttgtt tttgccgtat
     6001 ttttcccatt attttcattg ttgggatcca ttctttcttt ttaccgttat gctttcaagg
     6061 ctgttcttac gcccatgctc attgatgctt cgctgcataa cgatttgaga acaacgcttg
     6121 atgttgtatc tattccgtta gtaatttttg taattatatc tgtatctatt tcgattttag
     6181 ctgtcgtata tcgatttaaa aagataaaag tgaccaagcc tctgattcat tttgctgtca
     6241 gtgttttgtt gttgtgtatc ctgctaactg caaatggtcg ggtaacaaac agtttaatgc
     6301 agcgtttccc atttaatgtt tactataact tctctgaata caaaaaatta cgtcagacgc
     6361 tcgtcgccga aagaattgac cccgatcctg cattaaaagc aacctgtagt gattcaatga
     6421 aagtagtcat tgtgattgga gaagcattgc gggctgatca tttgtccctg aatggttacc
     6481 atcgtctcac caatccgcga ttgtcgaaaa gagaaaacat tttttcgttt tccaatatct
     6541 actcggaatt taccaatacc aacatgagtt taccccaaat tcttactcgt gccgacagta
     6601 tttatcccga gcgggctttt caagaaaaat cattcatcag tttgtttaaa aaatgtggct
     6661 attatacttc atggatttcc aatcaagacc ctgccgaaac atacgtatcg tttatcaatg
     6721 aatgtgatac tgttctttat ggccatcccg aaaaatcggt ttacaactat aatgaatggc
     6781 tggatgaaga tttgtttcca ttggtaaaag aaataaaaaa gaaagatttt ccatgcaact
     6841 tgtttgtttt acatacgata ggaagtcatt ggtattacaa caatcatttt tcatcttctt
     6901 ttaaggtgtt taccccggta acttcgagta ggattctttc ccaatgttcc tcagaagaat
     6961 tgatcaattc gtatgacaat acagtggttt ataccgattt ctttctcgac agtctgatca
     7021 gcatttttga aaatgaaaat gctgttgtga tttatttgtc ggatcatgga gagtcgctgg
     7081 gagaaaatgg aacatggctt catgccggcg atagtgaacc ttctcatcgt ccggcctgta
     7141 ttgtttggtg ttccgaaaaa tttgagaaaa aatatcccga taaagtggaa gcgttaagaa
     7201 ggaacaaaaa caaatattat cgcagtaatt ttgtttttca cagtgctttg gatgcagcga
     7261 atattcaaag taaagtgatg gataaaaagc ttagtatttt caatttgccg gctcaataat
     7321 ggaaaaggaa ttaattcata aacaatcatc gttgtctttc tttttgctga acaaatattt
     7381 ttgggcagca tgtgttgtgc tggggttaat tgtgaaggcg tatttcttac ccgtaaaaac
     7441 cggcgattac gtggtgtatc tcaaaccctg gattgatttc ataaaaactc atggctattt
     7501 ttcagcttta aaatatgatt tttccaatta tacgccggct tatctttatt ttctgacgct
     7561 tgttgccaaa atcccaattg aacctgtttt tgccattaaa attttttcca tagcttttga
     7621 atttctggct gcttattttt tgggaaaaat tgtttgtttg aaatatcaca attctctaat
     7681 tatcttgatt tcattggctg ttatcccctg gttaccaacc gtgatgctca acagcagtta
     7741 tttgtctcaa tgtgatgcca tttatgcttc ttttgtgatg ggcagcatct tttatgcttt
     7801 aaagaagcga ccatttttat cggtgctttt tctgggaata gcttttgcat ttaaaatgca
     7861 ggctgttttt gtattgcctt tcttctttgt ttttcttttg agaaaagaaa ttaaatggta
     7921 ttattttttt attgttcctg ccgtttattt ggtaagcatt ttaccggcat ggttcaatgg
     7981 ccgaagtttt gtcgaattaa tttctctgta tttttctcaa gccgattact atcggttgtt
     8041 gaccatgaat tttcctaata tttatatctg gttttcagga gcagattttg atactttttc
     8101 attagccggc atattattta ccgttttact gactttggtg gcaggctttt ggctgcgcac
     8161 tccaaaaata actttcaccc tcgaaacatg gataaaattt ctatttctaa gtttcattgt
     8221 ggttccgttt gttttgcccg gtatgcacga gcgctacatg tatctgggag atattttcgg
     8281 gttggcgtat ttgatgtttt tccccaaaaa atggtacttg ccgttcggaa ttttattggt
     8341 ttccacttat tcgtatatcc gatgttcgcg ctttagtgaa gttttaccca tgcaaccggc
     8401 ttttttctta tatttgttgg tgattttact gactttttac gactttgttg aaagtttaaa
     8461 attttcccga aaatgaatgc tgttcataaa gttaacttta cttttttagg cgtcatcatt
     8521 tttgtttatg tcttattgac agccggatgt ttgaataatt gttatttttg ggatgttgtc
     8581 cagcaggtct caaaggaagc ccattggtat tatcaaacga atttcaaata cttaatcatt
     8641 ccttcccaaa attctttgtc cgatattgta gcaacaggtt atcatccgcc tttgatggga
     8701 atgttgacgg cagcgttgtg gaaaattttt ggtgtgcatc tttggatttc tcatttgctg
     8761 acattgattt gtttttttgt gttgctttat cagacattaa agctggtcga aaagtttttc
     8821 cctgctcatc aggctggatg ggtcatgctg gtcgttttta tggaagcttc acttttggca
     8881 caattttcca tagcttcacc cgattttatt ttgttaactg ctttcatagc ttcgctcagg
     8941 ggattattag agcgaaatac atggctttcg tccattgcct tgatttttct tttcggaatc
     9001 aacatgcgcg gaatttttgc aggtattgcc ttatggatca gttttcagct ttttctttat
     9061 ctcaattcgg agaaaaaatt cagtttcaca ttgttggctg catcgattcg gccatttttg
     9121 ccggctatta ttttatcact gatctatttt attttttact ttcggatgaa tggctggttt
     9181 tccggagttt ctgaagaggg aggacattat tcgttgcccg aaaatatttc catgataggg
     9241 aaacattttt ttg
//
LOCUS       DAOSCH010000083         9075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5014_length_9075_cov_5.677716, whole genome shotgun sequence.
ACCESSION   DAOSCH010000083 DAOSCH010000000
VERSION     DAOSCH010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5014_length_9075_cov_5.677716"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="PLZ54_06815"
     CDS             complement(<1..483)
                     /locus_tag="PLZ54_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOJ66673.1"
                     /translation="MKKIKVGIVGGAGYTAGELLRILIYHPEVEIVFVNSSSNAGNPL
                     AEVHSGLIGETDLKFTAELPLQQIDVLFLCTAHGDSKKFLVEHDVPFSVKIIDLSTDF
                     REKRTDHDFVYGLPELNRKEILSAWHVANPGCFATAIQLGLLPLVKNGLLKDEIHVNA
                     I"
     gene            complement(1065..1421)
                     /locus_tag="PLZ54_06820"
     CDS             complement(1065..1421)
                     /locus_tag="PLZ54_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66674.1"
                     /translation="MYDLNRFFPEDNEMLTNFVEALFSANLQYSAMDLFRAGAESWKE
                     IDGAVKLSIQTLIKANLNPQMHFKFRFLTDIASGNIVQDWKMSKTGFVLTLLNIQGKN
                     PLLNKLKVEIIERYIN"
     gene            complement(1439..2380)
                     /locus_tag="PLZ54_06825"
     CDS             complement(1439..2380)
                     /locus_tag="PLZ54_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOJ66675.1"
                     /translation="MEYKDYYKTLGVDKNATQEEIKKAYRKLAVKYHPDKNPGNKEAE
                     NQFKMINEAYEVLSDPEKRKKYDELGTNWNKFGEGNEYRGSNPFEGFGNRGGSQFYYQ
                     DNFNDLFGGKNTGFSDFFEAFFGKGATSGFGKSNRQTEFKGQDYETEINLTLEEAYHG
                     TSRIIQIDNEKIRIKIKPGVNDGQVLRIKGKGAKGSNPNYNGDLLIKVKVLPHPIFER
                     IGNDLKMIHQINLFDAILGGKTIIETLNGKIIIDIPEGTQNNKILRIKGKGMPVYGKS
                     GEYGDLLIKLQVIIPENLTPEQKNMFRRLKENLHSQK"
     gene            complement(2469..2903)
                     /locus_tag="PLZ54_06830"
     CDS             complement(2469..2903)
                     /locus_tag="PLZ54_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOJ66676.1"
                     /translation="MALVKFSDQLPSLFDRLMNNDLFDWTFRNFSKTNTTLPSVNIKE
                     TNDAYRLEMAAPGLKKEDFKIEVKDGVLSISSEKVGEELKEGEEFTHREYSYQSFVRS
                     FTLPDNVEEDKIEAKYENGILCVNIPKKEEAKAKPVKQIEIQ"
     gene            complement(3201..4316)
                     /gene="dprA"
                     /locus_tag="PLZ54_06835"
     CDS             complement(3201..4316)
                     /gene="dprA"
                     /locus_tag="PLZ54_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOJ66677.1"
                     /translation="MDDSLKYKIALTMIHGIGNILAKNLIAYIGSVEGIFKEKRQNLA
                     KIPGIGEVLSKEIVSHSLIERAEKEIEFISKNQIKAIFFTEKDYPYRLKECPDSPILL
                     YCKGNTNFNEGKFVGIVGTRSATQTGKENCTKLVSDLAAMVPQLTIVSGLAYGIDIYS
                     HKAAVENHLPTIGVLGHGLDRIYPAAHRSTAIKMLAHGALLTEFISETNPDKPNFVKR
                     NRIIAGLCDALVVVESGIKGGALITAELANDYNRDVFAFPGRVDDIWSKGCNSLIKSN
                     KAFLMETAEDLLQIMNWEKLDSGSEIAPQVSMYFDLAEEEKEILFQLRQHADGLQVNE
                     LAIQMSKPFSKISSLLLQMEFKGLVKCLPGNIYRIIK"
     gene            complement(4309..4737)
                     /locus_tag="PLZ54_06840"
     CDS             complement(4309..4737)
                     /locus_tag="PLZ54_06840"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOJ66678.1"
                     /translation="MQHFQKKKIFFVKYWKSLLVLFVILYLSFASPSTFKDIPTFQHA
                     DKVAHFLMFALFTGVLINDFRHDKIVFTSKTEFLILSLAFPLFTGAIIEYLQGSVFQP
                     RSAEWLDFFSDTMGVVIGFLLMSALFHFLKKIKKIIRKDG"
     gene            4845..5636
                     /locus_tag="PLZ54_06845"
     CDS             4845..5636
                     /locus_tag="PLZ54_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOJ66679.1"
                     /translation="MRKYIFLSCLTLLLVSCSSVLITGRKQLNFVPEREILSMSSQSY
                     KEFIASAPLSTDKTNTALVKKIGSNIASAVEIYLRNNGLASEIANLSWEFNLVKDTSK
                     NAFCMPGGKVVVFEGILPIAKNEDGLAVVMGHEIAHAIAKHSNERLSQQMLAQFGAEL
                     TNILLSNKSEATRIGINTLYGIGVEMGVMLPYSRKQEYEADRLGLIFLAMAGYNPQES
                     VAFWQRMAAASSGSNIEFLSTHPSDQNRIKKLQEFIPEAMKYYNK"
     gene            complement(5897..6421)
                     /locus_tag="PLZ54_06850"
     CDS             complement(5897..6421)
                     /locus_tag="PLZ54_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004313451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOJ66680.1"
                     /translation="MAHIQSVRGIDPKIGKNCFLAETAVVIGDVTMGEGCSIWFNAVL
                     RGDVNSIKIGNHVNIQDGSVLHTLYQKSIIEIGDYVSIGHNVTIHGAKINDYALIGMG
                     AVVLDYAEIGEGAIVAAGALVLSNTVVPPYTLWAGVPAKFIKNVEPKQTNEINHRIAQ
                     NYSLYASWYQDKDK"
     gene            7501..8079
                     /locus_tag="PLZ54_06855"
     CDS             7501..8079
                     /locus_tag="PLZ54_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOJ66681.1"
                     /translation="MKTVCIYCASSQQIRKSFFEATDELAKNLVGAGYQIAYGGGSRG
                     LMGQLAESTLQSGGKIIGVIPRFMCEEEWNHNGLSELILVDTMHERKAMLAQMADAVV
                     ALPGGCGTMEELMEIITWKKLGIFTKPIVILNIEGYFDELLLMLKKAVAEHFMRPEHQ
                     SMWQVVDRSDQVVEAITNALLWNSDNRQWAVM"
     gene            8096..8938
                     /locus_tag="PLZ54_06860"
     CDS             8096..8938
                     /locus_tag="PLZ54_06860"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOJ66682.1"
                     /translation="MFQPDTTHILNTLSTLSVDTDNVKAIVQKSAGFKVTGIAVDTPY
                     PDSLIHFDTVSPVQLPFQGFEGILLPSTPENQVWVFLCLLGLFFLLIFFFWRYPFFFK
                     NTFRSYIKSSENNIFSKDDLNNTIPFFMVLYSLGVVSMFAAVFLHYPTEPFLPITCFK
                     FFLATTVFFLFKWLIIKLISYVFLDKVTFNMAKQIYFSLFFLFATILFPLLTIQIYLP
                     EKSTLTSIAALFLFIVAFIAFIIKFFQIFSTKFAAFFYIFLYLCTLEILPLILLVKTY
                     QRII"
     gene            8966..>9075
                     /locus_tag="PLZ54_06865"
     CDS             8966..>9075
                     /locus_tag="PLZ54_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016272242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOJ66683.1"
                     /translation="MKIKKILVSQPQPSSDKSPYYDISQKYNVKIDFKPF"
BASE COUNT         2735 a         1648 c         1468 g         3224 t
ORIGIN      
        1 aatggcattg acatgaattt catctttcaa caagccgttt tttaccaatg gcaacagtcc
       61 caattgaatg gctgtagcaa aacaaccggg attggctaca tgccaggcag aaagtatttc
      121 ctttcggttc agctcgggta aaccgtaaac aaaatcgtga tcagttcttt tttcacgaaa
      181 atcggtagaa agatcgatta ttttcaccga aaaaggaaca tcatgctcta caaggaattt
      241 tttactgtcg ccgtgtgctg tgcacaaaaa cagaacatct atttgctgta gtggcaactc
      301 cgcagtaaac ttcaaatccg tttcgcctat taatccgcta tgcacttctg ctaaaggatt
      361 accggcattg ctgctgctgt ttacaaacac aatttcaact tcaggatgat aaatcagaat
      421 gcgcaacaac tcgcctgccg tgtatcctgc ccctcccact atgccgactt taattttctt
      481 catattgaaa tttttgttcc ttagtacatg acaaaaaaat tgtagaaaaa tttatatttt
      541 ttattttcaa atatttaata acggggcttt gccccaaacc ctacttactt ttttgtcatg
      601 tacaaaattt ttgttcttaa aaaatttgct ttattcctct ctgtttacct gaaaaaccgt
      661 tttaaaacag gtttcaaagt taacatttta ttggtaatga tgcaccaaaa agtttaattt
      721 tcacttgaat ttacgatttt ttatttaggg gagatatgga aattaagaca ttgccatctg
      781 atttatgttc ccaacacttt ttttcttggt aataatattc agtcaaaatg cattgatagt
      841 atgaactttt ttaataatct atagtgcaaa ttccatccac acttaaaaaa tatattggca
      901 atatctgtca tgagctaact ttatccgttt tttcagaaat ttcgaatttt tccacaaata
      961 cttatcctca attgatatgc gttaaatatg atacagtttt ccaattttta gatatcggtt
     1021 ttttattcgc aatgtcgaaa aaacatctgt ttttccttct tcatttaatt gatatagcgt
     1081 tcaataattt ctacttttaa cttgtttaaa agtggatttt taccctgaat attgagtaat
     1141 gtcaatacaa aacccgtttt gctcattttc cagtcttgca ctatgttgcc acttgctata
     1201 tcggttaaaa atcgaaattt aaaatgcatc tgcggattca gattggcttt tataagcgtt
     1261 tgtatggata gttttactgc cccatcgatt tccttccatg attcagctcc tgctcgaaac
     1321 aaatccattg cactatactg aagatttgca gaaaaaagag cttctacgaa atttgtcaac
     1381 atttcattgt cttcggggaa aaatctgttc aaatcataca taatccatct attaattatt
     1441 atttttgtga atgcaaattt tcctttaatc tacgaaacat atttttttgt tcgggtgtaa
     1501 ggttctcggg aataatcact tgcaatttaa tcagcaaatc gccgtattct ccactttttc
     1561 catacacagg catacctttt cctttgatac gcagaatttt attgttctgt gtaccttcgg
     1621 gaatgtcaat tattattttt ccattcagtg tttcaataat tgtttttcca cccagtattg
     1681 catcaaacaa gttaatttga tgaatcattt tcaagtcatt gcctatccgc tcgaaaattg
     1741 gatggggtag tactttgact tttatgagca aatcgccatt atagtttgga ttgcttcctt
     1801 ttgctccctt tccttttatt cttaatactt gtccatcgtt aactcctggt ttgattttta
     1861 tacgtatttt ttcgttatcg atttgaataa ttctgcttgt accatgatat gcctcttcca
     1921 gggtaagatt gatttctgtt tcgtaatcct gccctttgaa ttcggtttga cgattacttt
     1981 taccaaatcc tgacgttgct cctttaccga aaaatgcttc gaaaaaatcc gaaaatccag
     2041 tgtttttacc accaaataaa tcgttgaaat tatcctgata gtaaaattga cttccgcccc
     2101 gattgccaaa accttcaaaa ggattacttc ctctatattc gttcccttca ccaaatttgt
     2161 tccagttggt tcccaattca tcatattttt tccttttttc cggatcggac agtacttcgt
     2221 aagcttcatt aatcatttta aactgatttt ccgcttcctt gttaccggga tttttatcag
     2281 gatggtattt tacggcaagt ttccggtaag cttttttaat ttcctcctga gtggcatttt
     2341 tgtctactcc aagtgtttta tagtaatctt tatattccat ataaaacaaa tatttcaagc
     2401 aacaaattgt taaaataaat ttaataaagg ggaacacaat agttcccctt catttgttaa
     2461 agtaaaattt attgaatttc gatttgctta accggcttag ctttagcttc ttcctttttg
     2521 ggaatattta cgcataatat tccattctcg tactttgctt cgattttgtc ttcttctaca
     2581 ttgtcgggca aagtaaaaga acgtacaaat gattgatagc tgtattccct gtgggtaaat
     2641 tcttctcctt ccttcagttc ttcaccaact ttttctgaag aaattgaaag caccccatct
     2701 ttaacttcaa tcttgaagtc ttcttttttt agacctggag ctgccatttc caacctgtat
     2761 gcatcgttgg tttctttaat gttaactgat ggaagagttg tgtttgtttt agaaaagttg
     2821 cgaaaagtcc agtcgaacaa atcattattc attaaacgat caaataatga tggcaattga
     2881 tctgaaaatt ttactaatgc catagttaaa ccctcctttc gttttataat tctaaatttt
     2941 actctacacc tcaagctatt caaattcaat accagagcat ttccaataat ttaatttgcc
     3001 cggtaaataa ctgtatttca gagtaatttt ttatcttatg gttgcagaag taatcgaaga
     3061 agggaataaa attattgaca gaatgacata tttttacgct gtaatgccaa actgtcaatg
     3121 tttgtttttt acttgactat atctgataga caattacttg tttcaccaca accactaatt
     3181 tcttctcttt tttatcagaa ttatttgatt attcgataaa tattacccgg taaacatttt
     3241 accaatcctt taaattccat ttgcagcaac agcgaagaaa ttttactgaa tggtttggac
     3301 atttggatag ccagttcatt tacttgaagt ccatcggcat gttgtctcaa ctgaaacaaa
     3361 atttcttttt cttcttctgc cagatcgaaa tacatggaaa cctgcggagc tatttccgaa
     3421 ccggaatcca gtttttccca attcattatt tgaagcaagt cttctgctgt ttccattaaa
     3481 aatgccttat tcgattttat cagactgtta catcctttgg accaaatatc atcgacacgg
     3541 ccgggaaaag caaatacatc cctgttataa tcgtttgcca actcggccgt tatcaatgcc
     3601 ccgcctttaa tacccgattc tactaccacc aaagcatcgc agagtccggc tataatccgg
     3661 ttacgcttta caaaatttgg tttatctgga tttgtttcac taataaattc ggtcagcaaa
     3721 gctccatgag caagcatttt gatagccgta gatctatgtg cagcagggta tatcctatcc
     3781 aatccgtgac ccagaacacc aatggtaggc aaatgatttt ctacagccgc tttatgagaa
     3841 taaatgtcaa taccataagc cagtccgctc actatggtga gttgaggtac cattgcggcc
     3901 agatcggata caagtttggt acaattttct tttcctgttt gagtggcact tcgtgttcca
     3961 actattccaa caaattttcc ttcgttaaag ttggtgtttc ctttacagta caacaaaatt
     4021 ggagaatcgg gacattcctt caatcgatag ggataatcct tttcagtgaa gaaaatggct
     4081 ttgatttgat ttttagaaat aaattcaatt tctttctctg ctcgttcaat aaggctgtgt
     4141 gatacaattt ctttggacaa aacctctcca ataccgggaa tttttgccag gttttgtctt
     4201 ttttccttaa aaattccttc cacacttcca atgtatgcaa tcagattttt ggcaagaatg
     4261 tttcctatgc catgaatcat cgttagggca attttatatt ttaaggaatc atccatcttt
     4321 tcggataatc ttttttattt ttttcaaaaa atgaaacaat gcagacatta aaagaaatcc
     4381 gataactact cccattgtat cagaaaaaaa gtcgagccat tctgctgagc gcggttgaaa
     4441 aaccgaacct tgcaggtatt caataatagc tccggtgaat aatgggaatg ccaaactcaa
     4501 tattaaaaat tcggttttgg aagtgaaaac aattttatcg tgtcgaaaat catttatcaa
     4561 aacccctgta aatagggcaa acataagaaa atgagcaacc ttatcggcgt gttgaaatgt
     4621 aggaatatcc ttaaaggtag aggggctggc aaacgacaga tataaaatta caaacaatac
     4681 caataaagat ttccaatatt taacgaaaaa tatcttcttt ttttggaaat gttgcataca
     4741 actaaaataa ttttatcaag aaaaaataat taaattttga ttatctttgc aaagataaaa
     4801 cctgttctca aaatactaaa atttgttttt caaatatcat tattatgaga aaatatattt
     4861 ttttaagttg tttgactctt ttgctggttt cctgttcaag tgttctcatc accggcagaa
     4921 agcaattgaa ttttgttccg gaaagagaaa ttttatcaat gagtagccaa agttataaag
     4981 aatttatagc ttcagctcct ttgtcaaccg acaaaacaaa tactgcatta gtaaaaaaaa
     5041 taggcagcaa cattgcatct gctgttgaaa tatatttgag aaataacggt ttggcgtctg
     5101 aaattgcaaa cctttcttgg gaatttaatt tggtaaagga tacttcaaaa aatgcttttt
     5161 gtatgccggg aggaaaagtg gtggtttttg aaggcatttt accgatagca aaaaatgaag
     5221 atgggttggc tgtagtaatg ggacacgaaa ttgcacatgc catagccaag cacagcaacg
     5281 aacgtctcag tcagcaaatg ctggcacaat tcggagccga attaaccaat attttgttgt
     5341 ccaataaatc ggaagctaca cggattggca ttaataccct ttatggaatc ggggtagaaa
     5401 tgggtgtgat gttgccttat tcaagaaaac aggaatatga agctgacagg cttggtttga
     5461 tttttttggc tatggccgga tacaatcctc aagaatctgt ggctttctgg caaagaatgg
     5521 ctgctgcttc ttctggcagc aacatagaat ttttaagtac gcacccttcc gatcaaaatc
     5581 gaataaaaaa acttcaggaa tttattcctg aagccatgaa atattacaac aaatgattgt
     5641 gatattatga ttcaccagta aaaatatgta tttaagcgca gatagttggt taatctgaaa
     5701 aaaagagttt agtgtttgat gcacctttcg atggatatct caaaaaattt ttttctttaa
     5761 attcttagat atttgctcat ctttcaaatt ccaacgaaaa attattaact atttgaacat
     5821 taatacggca ttttgatgag cttaaaagtt gccaaatgct gaaatttttt ttgtagcttt
     5881 atggcaagag agattattat ttatctttat cttgatacca gctggcatag agtgaataat
     5941 tttgggcaat gcgatgattg atttcgttag tctgtttagg ttccacattt tttataaact
     6001 tagcaggaac gccagcccac aaggtgtaag gaggaacaac agtattgctt aaaactaaag
     6061 ctccggcagc aactatggct ccctctccta tttcagcata atccaaaacc accgctccca
     6121 ttcctattaa tgcataatcg ttgatttttg ccccatgaat ggttacatta tgtccaattg
     6181 aaacataatc ccctatttca ataatcgatt tttgatacag cgtgtgcaaa accgatccat
     6241 cttggatatt aacatgattt ccaattttaa tggaattgac atcgccacgt aaaactgcat
     6301 tgaaccaaat gctacaacct tctcccattg taacatcgcc aatcactact gccgtttctg
     6361 ccaaaaaaca attttttcca atttttgggt caatccccct gactgattga atatgtgcca
     6421 tgtatatttt atttttcaag tgaaatttca aactgaattc aatttattaa tgcaacttta
     6481 gcattagaca aatttcgtaa taaaaattca tttgatgtca ataattttaa tttttttctt
     6541 aatctgttgt tcaacccaga ttactcatta gccaatttaa aaggaaagtc aaactcagaa
     6601 gagctgttct atagtccgga aaaccatgct cgcctttttt ttaaagcgat tttgagaagc
     6661 agtttgttgt tgatttttca aaatatgcac caatggcaaa agttttgtat ctaagtgtgt
     6721 agataaagtt ttttgcgttt tttctgcaaa atgtacattc taaaccacga cataagatta
     6781 tgggctatca ttaaaaaaat taaaacggct ttccttgcaa aaaagttttt tagattaaaa
     6841 ctgtcaaagc caaagtacta ttttaattcc tttattctgt tttcagcatg aactcttcct
     6901 ctgtatattc ttcatatgtc gggcgctgaa agtttcatgt tggttacgca agcagcataa
     6961 cgataatctc tgtaaatttc tttttcctca aacagacgaa agtgcttttc tttaattttt
     7021 gacgaacaat aacgattcta cgtggttttt ccaccatttg ttacaaaaaa aatcattcca
     7081 ccacatgatg ttatctcttt gtttgtaaag gagataccaa atttcataca cttctttttt
     7141 ttctattgct acgcattaga ctttcctaca aaattaaaac tttttctcta ttgcggattc
     7201 tggagttatc ttattggtag gagttattgt taagttttgg ataccgcaaa ccaaaggaaa
     7261 atagctggaa attagaaaaa atgctgggac tttgttaaat tctcactttt atgttgaact
     7321 tagcatttcg cacttgccta ttgactcttc attttatttg tgattcatca atcatataaa
     7381 tgaaaagaaa accgtatttt tgcaaaaaaa atgtcattcc cttaaaagga gattttttaa
     7441 aaaaaattga tgtttctctc tgaaaatttc atttaaaaaa cattcgactt gataaacaac
     7501 atgaaaaccg tatgcattta ttgcgcatca agccagcaga taagaaagag tttttttgaa
     7561 gcaaccgatg aactggctaa aaatttggtt ggggcgggtt atcaaattgc ttatggcggc
     7621 ggttcgcgag gactgatggg acaattggca gaaagtacct tgcagtcggg aggaaaaatt
     7681 attggtgtca ttccccgttt catgtgtgaa gaagaatgga atcacaatgg cctgtcagaa
     7741 ttgatattgg tcgacaccat gcatgaaagg aaagctatgc ttgcccaaat ggctgatgcg
     7801 gttgtagctt tgcccggtgg ttgcgggacg atggaagagt tgatggaaat tataacttgg
     7861 aaaaaactgg gcattttcac caaaccgata gtaattttga atattgaagg gtattttgac
     7921 gaattattgt tgatgttgaa aaaagcagta gcagaacatt ttatgcgacc tgagcatcaa
     7981 tctatgtggc aggttgttga tcgttctgat caggtggtcg aagccattac aaatgctttg
     8041 ctgtggaatt cggataatcg ccagtgggct gtgatgtaac tttaaaaaat tttttatgtt
     8101 tcaacccgat acaacacata tactaaatac cttgtcaaca ttatcggttg ataccgataa
     8161 tgtaaaagct attgttcaaa aatctgcagg ttttaaagtt acgggaattg cggttgatac
     8221 tccctacccc gattcgctca ttcattttga tactgtttct ccggttcaac ttccttttca
     8281 aggctttgaa ggaattttac ttccatccac gccggaaaat caggtatggg tgtttttgtg
     8341 tctgcttggt ttgttctttt tgctgatttt ctttttttgg cgttatccgt ttttttttaa
     8401 aaacacattt cgttcataca ttaaaagcag cgagaataac attttcagta aagatgattt
     8461 gaataatact attccttttt tcatggtgct ttattcatta ggtgtcgtga gcatgtttgc
     8521 tgccgttttt ttgcattatc ccaccgaacc ctttttaccg ataacttgtt ttaaattttt
     8581 tttggcaacc acagtttttt ttctctttaa atggcttatt attaaattga ttagttatgt
     8641 ttttttggac aaagttactt ttaatatggc taagcaaatc tatttcagtt tgttttttct
     8701 ttttgctact atattgtttc ctttgttgac aattcaaatt tatttgcctg aaaaatcaac
     8761 cctaacaagt attgcagcac tttttttgtt tattgtcgct tttatagcgt ttataattaa
     8821 attttttcag attttttcaa ctaaatttgc ggcttttttt tacatttttt tgtacctttg
     8881 caccctagaa attttaccat tgattttact tgtaaaaaca tatcagcgga taatttaaat
     8941 cgttcaatat taaaaagtta cagcgttgaa aataaaaaaa atacttgtat cacaaccaca
     9001 gccttcgagt gataaatcgc cttattacga tatcagtcag aagtataatg tcaagatcga
     9061 cttcaaaccc tttat
//
LOCUS       DAOSCH010000084         8596 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5368_length_8596_cov_6.781876, whole genome shotgun sequence.
ACCESSION   DAOSCH010000084 DAOSCH010000000
VERSION     DAOSCH010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5368_length_8596_cov_6.781876"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="PLZ54_06870"
     CDS             complement(<1..1362)
                     /locus_tag="PLZ54_06870"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004368387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOJ66684.1"
                     /translation="MTTNNQTQLGNTLWKIADDLRGAMNADDFRDYMLSFLFLRYLSD
                     NYEAAAKKELGKDYPVLNGNGGKTPLSVWYEQNPDDIIEFEKQMRRKVHYVIEPKHLW
                     NSIAYLAKTQSNELLQTLQEGFKYIETQSFESTFQGLFSEINLDSEKLGRSYEERNKK
                     LCTIIQKIAEGINQFSTDIDILGDAYEYLIGQFAAGSGKKAGEFYTPQRISDILSAIV
                     TLDSQDPSKGEKKKIERVLDFACGSGSLLLNVRKRMIEAGGTIGKIYGQEKNITTYNL
                     ARMNMLLHGVKDTEFEIHHGDTLLNDWDILNEMNPAKKLEFDAVVANPPFSYRWEPTE
                     AMGEDFRFKSYGLAPKSAADFAFLLHGFHFLSKEGTMAIILPHGVLFRGGSEERIRTK
                     LLKDGNIDTVIGLPANLFFSTGIPVCILVLKKCKKFDDVLFINAVDHYEKGKRQNILL
                     PEHI"
     gene            complement(1370..1933)
                     /locus_tag="PLZ54_06875"
     CDS             complement(1370..1933)
                     /locus_tag="PLZ54_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66685.1"
                     /translation="MDKFQNKYRIPSARHQNWDYRWDGAYFITICTKDRQHYFGKIEN
                     GKMILSNVGILADVFWHEIKNHNKNVELGEFVVMPNHVHDILILNNNETVAGNGNGVG
                     KPSVVKSNEQTIGQQRFQNIGKNSISSIIGSYKSAVTKHAHRSGFEFGWQTRFHDYIV
                     RDDDSFQTITHYILNNPTNWQEDKFFQ"
     gene            complement(1926..3206)
                     /locus_tag="PLZ54_06880"
     CDS             complement(1926..3206)
                     /locus_tag="PLZ54_06880"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOJ66686.1"
                     /translation="MSQNNENKLVPRLRFPEFAMEGEWEILPLSKLSYEITEKTKGRK
                     FKLMSITSGVGLVSQIEKFGKEIAGNSYDNYYVLKKGDFAYNKSSTKLYPEGEVAMLE
                     KEENGAVPNSIFTCFSFDKNKIYPIFAKYPFINNIHGRWLKKFIAVGARANGALQVNS
                     KDLFSIPFPYPSFKEQQKIAACLSSLDDVITAESKKLEVLKEHKKGLLQNLFPQEGET
                     VPKLRFKEFEDSGEWVERTLGEVSDNVMYGMNSAAIMFDGENKYIRITDIDDNTRLFS
                     PNPLTSPDGQLDDKYFVNVGDILFARTGASVGKSYLHQKENGKIYFAGFLIRFTIKNA
                     NPYFIYLQTLTEKYQKWVSKTSLRSGQPGINAEEFKSYPILISSLPEQQRIASCLSSL
                     DNLINAQSQKIETLKLHKKGLLQGLFPDVNEHNG"
     assembly_gap    3259..3358
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3359..3682)
                     /locus_tag="PLZ54_06885"
     CDS             complement(<3359..3682)
                     /locus_tag="PLZ54_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HOJ66687.1"
                     /translation="MYILECADGSYYTGSTNNLELRLAQHQAGEGANHTKKHLPVKLV
                     YFEEYARIDQAFYREKQVQGWNRKKKEALINGMPQKLHELAECMNETHYKNYSNGGFG
                     SAQPPQ"
     gene            complement(3690..6704)
                     /locus_tag="PLZ54_06890"
     CDS             complement(3690..6704)
                     /locus_tag="PLZ54_06890"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017142418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HOJ66688.1"
                     /translation="MTKEIQIEESLITKLTDLKYTYRSDIHDRVSLENNFREKFQALN
                     RVHLTDSEFARLRDDIINADVYVSSKRLREINTFMREDGTPLHYTLVNIKDWCKNEFE
                     VINQLRINTQNSYHRYDVILLINGIPVVQIELKGLEVSPRRAMQQIVEYKTDPGNGYT
                     NSLLCFMQLFIVSNRANTYYFANNNNNHFSFNADEQFLPIYQLASEDNKKITHLDDFS
                     EKFLSKCTLGQMISRYMVLVASEQKLLVMRPYQIYAVKAIVDCIHQNRGNGYIWHTTG
                     SGKTLTSFKASTLLKDNPDIEKCLFVVDRKDLDRQTREEFNKFQEGCVEENTNTEMLV
                     RRMLSDDYANKVIVTTIQKLGLALDPNIKNNYKERLQPLRNKRIVFIFDECHRSQFGE
                     NHKAIKEFFPNAQLFGFTGTPIFDENATYKQIDGTVGSFVTTKDIFQKQLHAYTITHA
                     IEDRNVLRFHIDYFKPETDITIGSREHKTAVANSILKKHDAATHNRRFNAILATASIN
                     DAIEYYNLFKDIQAKKIKKDENFIPLNIACVFSPPAQLITKEGDQQSQKNAADIRQMQ
                     EDLQQEKEDNKVEPEKKKKALQAIIADYNKQYGTNHTINEFDLYYQDVQQRIKFQKYS
                     NADYPHKNKIDMVIVVDMLLTGFDSKYLNTLYVDKNLKYHALIQAFSRTNRTLHSTGT
                     EVTIKPYGNILDFRQQQEEVDKAIALFSGEDTGRAKEIWLTESAPEIIEKYREAVKAM
                     EKWMESKNIVAEPQEVYNIKGDAARIEFINRFKEVQRLKTQLDQYTDLDDEQKAKIEQ
                     LMPEEQLRSFRSSYLEIAKQLKEVQQKEGDKAPDNVKQLDFEFVLFASALIDYDYIMN
                     LIAKYTQGTPKKQKMTRAQLIGALSSSANLMEEREDIIEYIETLEVGKALNEQEIKEG
                     YQLFKAEKIAKELAHIAQKNGLELSALRQFTESIISRMIFDADKLSELFAPLDLGWKE
                     RTKRELALMNDLVPILKKQAEGREISGLKAYE"
     gene            complement(6835..7374)
                     /locus_tag="PLZ54_06895"
     CDS             complement(6835..7374)
                     /locus_tag="PLZ54_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOJ66689.1"
                     /translation="MKTKKLSFLLINTVIMIGLFSCSTIPKGVKAVAPFDKGKYLGKW
                     YEIARIDFKYEKDLNNTTAEYSLNANGTIKVINKGYNPKKGEWEQAIGKAKFVGNDTI
                     GMLKVSFFGPFYSGYNVIALDPDYKYALVGGASYKYLWILSRETSIPDEIKYKYLKIA
                     EDFGYNTSNLLWIVHDNQK"
     gene            complement(7566..8510)
                     /locus_tag="PLZ54_06900"
     CDS             complement(7566..8510)
                     /locus_tag="PLZ54_06900"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HOJ66690.1"
                     /translation="MIIDFKEIPQANGGDGLQDTFELFSRDFFEILGYEILQHPDRGA
                     DGKKDLIIQETRIGVSGTTKIKWLVSCKHYAHTGKSVSDTDEPNILDRVSVHNCDGFI
                     GFYSTLPATSLGKNLDGLKSKTNIQSFDREHIEKILLESPQGLRLASRYFPISFEKYK
                     TENPKPAKIFSDEPTINCEYCNKNLLEDGSGIFVTLRKFTDYDAKVVTKNPYEEAYFS
                     CKGHCDTVLKNKYLQKGNYIDEWIDISDYLTPTGYIKKSMAWMNGMNIDNEKLEKNAF
                     DKLKKLFLNCFPYISREQTTEEKERIKMYLQNGWDDLL"
BASE COUNT         2261 a         1706 c         1531 g         2998 t
ORIGIN      
        1 aatatgttcg gggagcagga tattctgtcg ttttcctttt tcatagtggt caacggcatt
       61 gatgaacaat acatcgtcaa actttttgca cttctttaaa accaatatgc aaacgggaat
      121 accagttgag aaaaaaaggt tagcaggcaa accaattacc gtgtcaatgt ttccgtcctt
      181 tagcaatttt gtacggatac gttcttccga accacctctg aataaaacac cgtgaggcag
      241 aataattgcc attgtaccct ctttgcttaa gaaatgaaaa ccgtgtaaca gaaatgcaaa
      301 gtcggctgcc gattttggtg caaggccgta actcttgaaa cggaaatctt ctcccattgc
      361 ttccgttggc tcccaacgat agctgaaagg tggattggca acaacggcat caaattccaa
      421 tttcttagca ggattcattt cgtttaaaat atcccaatca ttgagcaaag tatctccatg
      481 atgaatttcg aactcggtat ccttaacgcc atgcaaaagc atattcatgc gggcaaggtt
      541 gtaagtggta atattttttt cctgcccgta tattttcccg atggtgccgc cggcttctat
      601 cattcttttg cggacgttga gcaacaacga acccgaacca caggcaaaat ccaatacgcg
      661 ttcaatcttt ttcttttcgc ctttgctcgg gtcctggctg tcgagcgtta caatggccga
      721 aagaatatcg gatattcgtt gtggtgtgta aaattctcct gcctttttgc ctgatcctgc
      781 ggcaaattga ccaatcagat attcgtatgc atcgcctaaa atatcgatgt cggtcgagaa
      841 ttgattgatg ccttccgcaa ttttctgaat aatggtacaa agttttttgt ttcgttcctc
      901 gtagctcctt cccaactttt cggaatcgag attgatttcg gaaaacaaac cctgaaatgt
      961 actttcaaaa gattgggttt cgatgtattt aaaaccttct tgtaaagttt gaagtaactc
     1021 attgctttgg gttttagcca ggtatgcaat gctattccat aaatgtttag gttcaatcac
     1081 ataatgaacc tttctacgca tttgcttttc aaattcgatt atatcgtcag gattttgttc
     1141 gtaccacaca gacaaaggag ttttaccacc attgccattt aataccggat aatctttccc
     1201 caattccttt ttggctgccg cttcgtagtt gtcgctcaaa tagcgcaaga acagaaacga
     1261 aagcatataa tcgcggaaat cgtcggcgtt cattgctcca cgcaaatcgt ctgcgatttt
     1321 ccaaagggta ttgcctaatt gagtttggtt atttgttgtc atattctatt tattgaaaaa
     1381 atttatcttc ctgccagttt gtcgggttgt ttaagatata atgggttata gtttgaaatg
     1441 agtcatcatc tcggacaatg taatcatgaa atcgggtttg ccaaccgaat tcaaatcctg
     1501 aacgatgggc atgtttagta acggcagatt tgtaggaacc tataatagac gaaatggaat
     1561 ttttaccgat gttttgaaac cgttgttgtc cgatggtttg ttcgtttgat tttacgaccg
     1621 atggtttgcc aaccccattt ccattgccgg caacggtttc gttattgttt aaaattaaaa
     1681 tgtcatgaac atggttgggc atcaccacaa attcgcctaa ttccacattt ttgttgtggt
     1741 ttttaatttc gtgccaaaat acatctgcca atatcccaac attagataaa atcatttttc
     1801 cattttcaat ttttccaaaa taatgttgtc tgtctttggt gcaaatagta ataaaatatg
     1861 ccccgtccca acgatagtcc caattttgat ggcgggcaga aggtatgcgg tatttgtttt
     1921 gaaatttatc cattatgttc atttacatca ggaaacaacc cctgcaacaa tcctttcttg
     1981 tgcaatttta aggtttctat cttttggctc tgggcgttga ttaaattatc taatgaggaa
     2041 aggcaggatg caattctctg ttgttcggga agcgatgaaa taagaatagg ataagattta
     2101 aattcttctg cgtttattcc cggttgtcct gaacgtaaag aagtttttga aacccatttt
     2161 tgatattttt ctgttaaggt ttgaagataa ataaaatatg gatttgcatt ctttattgtg
     2221 aaacgaatta aaaaacctgc gaaataaatt ttaccgtttt ctttttgatg taaatatgat
     2281 tttccaacac ttgcccctgt tcttgcaaaa agaatatcac caacatttac aaaatattta
     2341 tcatccaatt gtccatcggg agatgttaaa ggattaggcg aaaacaatct tgtattatca
     2401 tcaatgtctg taatacgaat gtatttgttt tctccgtcaa acataatagc agccgaattc
     2461 attccataca taacattatc tgaaacttct cccaatgttc tctctaccca ctccccacta
     2521 tcctcaaact ccttaaaccg caacttcggc acggtttccc cttcctgcgg aaaaaggttt
     2581 tgcagtagcc cttttttatg ttctttcagc acttccaatt ttttgctctc ggcagttatg
     2641 acatcatcta aagacgaaag gcaggcggcg attttttgtt gctctttaaa agatggataa
     2701 ggaaatggaa ttgaaaataa atcctttgag ttaacttgaa gagcaccatt tgccctagca
     2761 ccaactgcaa taaatttttt taaccaacga ccatgaatat tgtttataaa tggatattta
     2821 gcaaaaattg gatatatttt gtttttatca aaactaaaac acgtaaatat gctattagga
     2881 acagctccat tttcctcttt ttcaagcatt gcgacttcac cttctggata tagtttggtt
     2941 gagcttttat tgtaagcaaa atctcctttt ttcaacacat agtaattgtc gtaagaattt
     3001 ccagcgattt ctttcccaaa tttttcaatt tgcgaaacaa gacctactcc agaagtaata
     3061 ctcatcaatt taaatttcct accttttgtc ttttccgtaa tttcgtaact taatttactc
     3121 aaaggcagta tttcccattc accttccata gcaaactccg ggaatctcag ccttggaacc
     3181 aatttatttt cgttgttctg actcatacgc tttcagttct gatatttact gcccttcggc
     3241 ttgtttatgt gttgtgctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     3361 gaggtggttg agcggagccg aaaccaccat tactataatt cttgtaatgc gtttcattca
     3421 tgcattcggc caattcatgt agtttttggg gcatcccatt tattaatgcc tctttctttt
     3481 tccgattcca gccctgcact tgcttctcgc gataaaatgc ctggtcaatt cttgcatatt
     3541 cttcaaaata taccaattta accggtaaat gctttttggt atgattagcc ccttcgccgg
     3601 cctgatgttg tgctaaccgc aattccaaat tattggtact cccggtatag taactgccat
     3661 cggcacattc caatatgtac atccaacctt tactcatacg ctttcaaacc gctaatttcc
     3721 cgtccttctg cctgtttttt gagtatggga acaaggtcgt tcatcaaggc aagttcccgt
     3781 ttggtgcgtt ctttccagcc caagtctaag ggtgcaaaaa gttcacttag tttgtcggca
     3841 tcaaaaatca ttctgctgat aatgctttcg gtaaattgtc gcaatgctga tagctccaaa
     3901 ccgtttttct gtgcaatatg ggctaactct ttggctattt tttctgcttt aaacaattgg
     3961 taaccctctt taatttcctg ctcatttaat gctttgccca cttccagggt ttcgatgtat
     4021 tcgataatat cttcccgctc ttccattagg ttagcacttg aactcaatgc accaatcaat
     4081 tgggcacggg tcattttttg ctttttcggt gtgccttgcg tgtatttggc aatcaggttc
     4141 atgatgtagt cgtagtctat caaggctgaa gcaaacagca caaattcaaa atcgagttgc
     4201 ttaacattat caggtgcttt gtcaccttct ttttgttgaa cttctttgag ttgtttggca
     4261 atctccagat acgaactgcg gaacgaacgc aattgttctt cgggcatcaa ctgttcaatc
     4321 tttgcttttt gttcatcgtc caggtcggtg tattggtcaa gctgagtttt cagccgttgc
     4381 acttctttaa aacgattgat aaactcgatg cgtgcagcat cgcctttgat attgtaaact
     4441 tcctgcggtt ctgccacgat gtttttcgat tccatccatt tttccatggc ttttactgcc
     4501 tcccgatatt tctcaattat ttccggagca gattccgtca accatatttc tttggctcgc
     4561 cctgtgtctt ctccgctgaa taaggcaatg gctttgtcca cttcttcctg ctgttggcgg
     4621 aaatcgagga tgtttccgta aggcttaatg gtaacttccg tgcctgtgga atgcaatgtt
     4681 cggttggtgc ggctgaaagc ctgaatcaat gcgtggtatt tcaggttttt gtccacatac
     4741 aaagtgttca ggtattttga atcaaaaccg gtaagcaaca tatccaccac aatcaccata
     4801 tcaattttgt ttttgtgcgg atagtcggca ttactgtatt tctgaaattt aatgcgttgc
     4861 tgtacatcct gataatacag gtcgaactcg ttaatcgtgt gattcgttcc gtattgcttg
     4921 ttgtaatcgg caataatggc ttgcagcgct ttcttctttt tttcgggttc tactttgttg
     4981 tcctcttttt cctgctgtaa atcttcctgc atttgcctta tatcggctgc atttttttgg
     5041 ctttgttggt cgccctcttt agtaataagt tgtgcaggcg gagaaaacac acaagcaatg
     5101 tttaaaggaa tgaagttttc atctttcttg attttttttg cctgaatgtc tttgaacagg
     5161 ttgtaatact caatggcatc attgatggaa gccgttgcca atattgcgtt gaaacgtctg
     5221 ttatgagtgg ctgcatcgtg ttttttgagg atggaattgg caaccgccgt tttatgttcc
     5281 ctgctgccta tcgtaatatc tgtctcaggc ttgaaatagt cgatgtggaa acgcaaaacg
     5341 tttctatctt cgatggcatg agtaatggtg taggcatgca gttgcttttg aaaaatatct
     5401 ttggtagtta caaaagagcc aactgttccg tcaatttgct tatacgtggc attttcgtcg
     5461 aaaatgggtg ttccggtaaa tccgaataac tgtgcattgg ggaaaaattc tttgatggct
     5521 ttgtggtttt cgccaaactg cgagcggtgg cactcgtcaa aaatgaaaac aatgcgttta
     5581 ttcctcaggg gttgtaagcg ctctttgtag ttgtttttaa tgtttgggtc taatgccagt
     5641 cctaattttt gaatggtcgt aacgattact ttgttggcgt aatcatcgga aagcatacgc
     5701 cttaccagca tttccgtgtt ggtgttttct tcaacgcaac cttcctgaaa tttattgaac
     5761 tcctcacggg tttgtcggtc aaggtctttg cggtcaacca caaacaaaca tttttcgatg
     5821 tcgggattgt ctttgagtaa agttgaggct ttgaatgaag taagcgtttt accgcttcct
     5881 gtggtgtgcc aaatgtagcc gttgcctctg ttttgatgaa tacagtcaac aatagccttt
     5941 accgcataaa tttggtaggg ccgcattacc aacaattttt gttcgcttgc caccaaaacc
     6001 atgtatctgc taatcatttg ccccaaggtg catttgctca aaaacttttc cgaaaagtcg
     6061 tctaagtgtg tgattttttt attgtcttca cttgctaatt ggtaaattgg caaaaactgt
     6121 tcgtctgcat taaagctgaa atgattattg ttattattgg caaaatagta agtattagca
     6181 cggttgctca caataaacaa ctgcatgaaa caaagcaacg aattggtata accgttgccc
     6241 ggatcagttt tgtactcaac aatctgctgc atggctctgc gtggactcac ttctaaacct
     6301 ttcaactcta tttgtacaac agggattcca ttaatcagca aaatcacatc ataccggtga
     6361 tagctatttt gggtatttat ccgaagttga ttgataactt caaactcatt tttgcaccaa
     6421 tctttgatgt tcaccaatgt gtaatgcaac ggcgttccat cctcgcgcat aaacgtattg
     6481 atttccctca aacgttttga tgaaacatat acgtcagcat ttataatgtc gtcccgaagt
     6541 cgtgcaaatt cggaatcagt caaatgaacc cgattaagtg cttgaaactt ttcacggaaa
     6601 ttattttcca aactaaccct gtcatgtata tccgatctgt aagtgtattt taggtcagtc
     6661 agtttggtta ttaagctttc ttctatttga atttcttttg tcattattct tctttcaata
     6721 tttctttaag attaactgcc aacattattt gctatcccat aaaatatttg aaggttttac
     6781 ttgttgttgt tttttgtttc aaatcaaggt aaaagtgtca gttgtgtgct aaccttattt
     6841 ttgattgtca tgtacaatcc acaaaagatt tgaagtattg tatccaaaat cctctgcaat
     6901 ctttaaatat ttatatttta tctcatcagg tatactcgtc tctctagata aaatccatag
     6961 atacttgtag cttgctcctc ctaccaatgc atatttataa tcagggtcga gcgcgattac
     7021 attatatcct gaataaaacg gaccaaaaaa tgacacttta agcattccaa tcgtatcatt
     7081 tccaacaaat ttggcttttc ctatcgcttg ttcccattct ccctttttag gattatatcc
     7141 tttattaata actttaattg taccattggc atttaaagaa tattcagcag tagtattgtt
     7201 taaatctttt tcatatttaa aatcaattcg agcaatttca taccatttgc caagatactt
     7261 tcctttatca aaaggagcaa cggcttttac tcctttcggg attgttgaac aagaaaacaa
     7321 tccaatcata atgacagtat taataagtaa aaatgataat tttttagttt tcatttgttt
     7381 ttttatttat ttgttattca tttcctgttt ttcagggttg cacacaacgg tggcgggttt
     7441 atgaagttgg cgttttcgaa gcacgaaact gtctgctaac accacacttg ataagaagca
     7501 caaagcttta agtttgcacg tcaccccgcc tgacgcaaaa cccgtgttag cggttcggct
     7561 ttattctaca aaaggtcgtc ccaaccattt tgtaaataca tttttattct ttctttttct
     7621 tctgttgttt gttctcgtga aatgtatgga aaacaattaa gaaatagttt tttcaactta
     7681 tcaaaggcat ttttttcaag tttttcgtta tcaatattca ttccattcat ccaagccata
     7741 gactttttaa tataacctgt tggtgtcaaa taatctgaaa tatcaatcca ttcgtcaatg
     7801 taatttccct tttgcaaata cttatttttt aaaacggtat cacaatgtcc tttacaactg
     7861 aaatatgctt cttcatatgg atttttagtc acaacttttg catcgtagtc agtaaacttt
     7921 cttaatgtaa caaaaatacc tgagccgtct tccaaaaggt ttttattgca gtattcacaa
     7981 ttgatagtcg gttcgtcaga aaatatttta gctggtttag gattctctgt tttatacttt
     8041 tcaaaactta tcgggaaata tctacttgct aatcttaaac cttgaggcga ttcaagtaat
     8101 attttttcta tgtgttctct gtcaaaactt tgaatgtttg ttttggattt aagtccatca
     8161 agatttttac ctaatgaagt cgctggtaaa gtggaataaa agccaataaa tccgtcacaa
     8221 ttatggactg aaacacggtc aagtatattt ggttcgtcag tgtccgaaac agatttacct
     8281 gtatgtgcat aatgcttgca actaactaac catttgattt ttgttgtccc cgaaacgcct
     8341 attctagttt cttggattat caaatccttt tttccgtccg cacccctgtc tgggtgttgt
     8401 aaaatttcgt aaccgagaat ttcaaaaaaa tcccttgaaa aaagttcaaa ggtgtcttga
     8461 agtccgtcac caccatttgc ttgtggtatt tctttgaagt ctataatcat tgtctgttta
     8521 tgttatgtcg ttttaagctg accgctaacg gtttggcgct tggcgcagtg gcggatttcg
     8581 gagcacaaaa ctgtca
//
LOCUS       DAOSCH010000085         8485 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5450_length_8485_cov_6.237367, whole genome shotgun sequence.
ACCESSION   DAOSCH010000085 DAOSCH010000000
VERSION     DAOSCH010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5450_length_8485_cov_6.237367"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..1133
                     /locus_tag="PLZ54_06905"
     CDS             108..1133
                     /locus_tag="PLZ54_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOJ66691.1"
                     /translation="MKCFASDNYSGIHPDILKAIVEANADHEISYGDDTYTQRAKKLF
                     QQKLGNVEVLFVFNGTGANIVCLKCCTLPFQSIVCAQTAHILTDECGAVFQNIGCSIM
                     PVHTPDGKLTPELISPVLVHKGNVHSAQPKVISISQTTEVGTLYDTEELKALCDFAHK
                     NNMLVHVDGARISNAVAALGKSLKEVTVDCGVDIMSFGGTKNGMMMGEAILIFNDELN
                     ANARFYQKQSLQLYSKNRFIAAQFMALLKNDLWLKMATHSNKMASLLAEKMRAIPEVQ
                     ITLPVQANAVFAIIPEKVIEPLREKFRFYTWDFQTNELRWMCSFDTKPEEIDEFAETL
                     KKLLADR"
     gene            1324..2157
                     /gene="dapF"
                     /locus_tag="PLZ54_06910"
     CDS             1324..2157
                     /gene="dapF"
                     /locus_tag="PLZ54_06910"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOJ66692.1"
                     /translation="MKFTKMHGIGNDYIYINGFEEQLENPGELSIRLSDRHKGVGSDG
                     LVIILPSSKADFRMQMFNADGSEAEMCGNAARCIGKYVYEKGMTDKTELTLETLAGIK
                     TLQLFLDERNNVVSVTVDMGKPVLEAEKIPTTLPGKQIVNFPVSFDGINYAITCVSMG
                     NPHTVIFTENIANMDIEKMGKKIENAPIFPRRTNVEFIEVIDKSRIEMRVWERGSGET
                     MACGTGACASVVAGVLNGLVSRKATVELLGGNLEIEWNENDEHVYLTGPAETVFEGEI
                     S"
     gene            2236..3459
                     /locus_tag="PLZ54_06915"
     CDS             2236..3459
                     /locus_tag="PLZ54_06915"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HOJ66693.1"
                     /translation="MAYINENFIKIPPTYLFSEIAKRVEQHKAENPSAKIIRMGIGDV
                     TLPLPDASVDAMIKAAEEQRHAETFRGYGPEQGYEFLRQAIVENDFRARGIKLDVDEV
                     FVSDGAKSDTGNIGDIFGIDNCVAITDPSYPVYVDTNAMAGRAGEPNEFNEWNGLTYL
                     PCNEANNFVPQLPDKKVDMIYLCYPNNPTGTTLSKQQLQVWVEYAKANRSVILFDAAY
                     EAFITEENVPHSIYEIEGAKEVAIEFRSFSKTAGFTGTRCGYTVVPKELVGYTHQGEA
                     VALNKLWLRRQSTKFNGTSYIVQRGAEATFTPEGRKQVKQLIEYYLGNARIIREGVAK
                     LGLKVFGGVNAPYVWVKAPDNMKSWEYFDFLLKQKNIVTTPGAGFGRSGEGYIRFSAF
                     GSRENTIEAMERLIK"
     gene            3641..4375
                     /locus_tag="PLZ54_06920"
     CDS             3641..4375
                     /locus_tag="PLZ54_06920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOJ66694.1"
                     /translation="MNTSSSQRKISDPELGDIYFNRSQRATRINIQILEDKLKVTLPM
                     RASEKDAMRFIDSVREKILKKRAQVKEKIRKNKQIIDEQSPFRALTFVTDIKPTHRED
                     IYCCLKNQVLSIEIPSEVDCREEQMQNYLHRIIARYLRLEAKRVLPQRTRLLADKFGF
                     RFSTVKIQSSRTRWGSCSAKGNINLSLYLMALPAHLIDYVILHELCHTRELNHSTDFW
                     RWLNRVTDNQSDRLKEELKNFNFNWD"
     gene            4427..5752
                     /locus_tag="PLZ54_06925"
     CDS             4427..5752
                     /locus_tag="PLZ54_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOJ66695.1"
                     /translation="MNWKQLITTKRLGMEAYHEKRQDNRSEFQRDYDRLIFSPPFRRL
                     QNKTQVFPLPGSVFVHNRLTHSLEVSSVGRSLGINVARQIKEKNNLPDAFIDEVGSIV
                     SAACLAHDMGNPPFGHSGEKAIATYFSEGEGQILQEKMLPEEWSDLISFEGNANTFRV
                     LTHQFRGRRPGGFVLTYSTLASIVKYPYASVFSDPQRQKFGFFHSEKSDYEKIAHELG
                     IPHSFSQPDKYVRYPLVYLVEAADDICYQIMDIEDAHKLKILSTDETKALFLSYFEGK
                     KLERKLQTIAMVDDVNEQIAYLRSSVIGQLIDNCSSVFLENEPQILEGSFSSTLIKLL
                     PPVSLKAYQNCSKTAFEKIYCSSEVLDVELAGYKIIFTLLENLVKAVLSPEKAYSQQL
                     LMRIPKQYEIDQPTTYGKVMAILDYISGMTDVYALDLYRKITGMNIPTL"
     gene            complement(5782..6474)
                     /locus_tag="PLZ54_06930"
     CDS             complement(5782..6474)
                     /locus_tag="PLZ54_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOJ66696.1"
                     /translation="MKKLFLLSIMMVFTFFSFQSGQNKKGVEKSQNTLPMDLKTFDTI
                     QLLAPDTMGGKSLMQTFQHRKSDREFKQTPLSLKHLSEILWAAYGINRPDGKRTVPSA
                     RALYPLQVYAVLPNGIYRYEPQTHRLVPVVKGDYRKLAGLQDFVYTAPMNLIFIADFN
                     KYNLTQKEPDANSIRMVSLDAGHCTQNVYLYCASEGLRCVVRAGAKADELLSLLSLDS
                     HYRFIVAQTVGY"
     gene            7140..7556
                     /gene="mce"
                     /locus_tag="PLZ54_06935"
     CDS             7140..7556
                     /gene="mce"
                     /locus_tag="PLZ54_06935"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOJ66697.1"
                     /translation="MKPKKIEHIGIAVKSLETAIPFFESVLGLTCYAIEEVKDQKVKT
                     AFFKVGDTKIELLESTDPEGPIGKFIEKRGEGVHHIALAVENVQQALNEASDKGVMLI
                     DKQPRKGAEGLNIAFIHPKSTFGILTELCENPNINC"
     gene            7593..>8485
                     /locus_tag="PLZ54_06940"
     CDS             7593..>8485
                     /locus_tag="PLZ54_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOJ66698.1"
                     /translation="MNNLEKLEQLISLRAKAKLGGGEKKIESQHKKGKYTARERIEML
                     LDDGSFEELDMFVTHRCTNFGMENEKYLGDGVVTGHGTIDGRTVYVYAQDFTVFGGSL
                     SEMMAQKICKVMDMAMKMGAPIIGINDSGGARIQEGVTSLAGYAEIFERNILASGLVP
                     QISAIFGPCAGGAVYSPALTDFILMTEENSYMFVTGPKVVKSVTGEDITTEDLGGANV
                     HSSKSGVSHFKVESEEEGLLMIRKLLSYLPQNNLEEPPLIQNYDPIDRHEDKLNTIVP
                     DNPNLPYDMKEIIHSLVDNGE"
BASE COUNT         2740 a         1515 c         1727 g         2503 t
ORIGIN      
        1 attatacaaa tttttataac tattttctga aaaatatacc ataatttatc cttttaattt
       61 aggatacttt gcggattatc attaaacttt tgtaaaaaag aaattatatg aaatgttttg
      121 caagcgacaa ttattcgggc attcatcccg atatccttaa agccattgta gaagccaatg
      181 ccgatcatga aatttcttat ggcgatgaca cttacaccca acgggcaaaa aaactttttc
      241 agcagaagtt gggaaatgtg gaagttttgt ttgttttcaa tggtacaggt gccaatattg
      301 tttgtttaaa gtgctgtaca ttgccctttc aatcgattgt gtgtgcccaa acggctcata
      361 ttttgactga cgaatgcggt gcagtttttc aaaatatcgg atgttcaatt atgccggtgc
      421 atacgccgga cggaaaactt actcctgaac tgatttcccc ggttttggta cacaaaggca
      481 atgtgcacag cgcacaacca aaagtgattt ccattagcca aaccacagaa gtgggaacgc
      541 tatatgacac cgaagaactg aaagcattat gtgattttgc acataaaaac aacatgcttg
      601 ttcatgtaga tggcgcaaga atttccaacg cagtggcagc gctgggaaaa agtttaaagg
      661 aagttaccgt tgattgcggg gtggatatta tgagttttgg cggcacgaaa aatggaatga
      721 tgatgggaga ggcaattttg atttttaacg atgaactgaa tgccaatgcc cgtttttatc
      781 agaaacaatc tcttcaattg tattcaaaaa atcgttttat agcagcacag tttatggctt
      841 tgctcaagaa cgatttgtgg ctgaaaatgg caacacattc caataaaatg gcaagtttac
      901 tggcagaaaa aatgcgcgct atacctgaag ttcaaataac tttaccggtg caggctaatg
      961 ccgtttttgc cataattccc gagaaagtga ttgagcctct tcgggaaaaa tttcgatttt
     1021 acacttggga ttttcaaacc aatgaattgc gctggatgtg ctcttttgat acaaagcccg
     1081 aagaaattga tgaatttgca gaaacattga aaaagttatt ggccgatagg tgatgaaagt
     1141 tcaaaatatt caatcacagc cattaaattt gaattttgtt tgaattctct caaaaaggaa
     1201 gctgttttct gataatttat aagtaaacac agcttgtttt gaaaatcgca attataattt
     1261 tctcctgtcc tgatttaatt ttttatcttt gtcatcgaaa aaagtacata aattattttc
     1321 atcatgaaat ttacaaaaat gcacggaatc gggaacgatt acatttatat caacggattt
     1381 gaagaacagc ttgaaaatcc cggcgaatta tccatacgct tgagcgacag acacaaagga
     1441 gttggatccg atggattggt aattattctt ccttcttcaa aagccgattt tagaatgcaa
     1501 atgttcaatg ccgatggctc ggaagcggaa atgtgtggaa atgccgcgcg ctgcataggt
     1561 aaatatgtct atgaaaaagg tatgacagac aaaaccgaat tgacccttga aacgttggct
     1621 ggtatcaaaa ccctgcaact ttttcttgac gagagaaaca atgttgtttc cgttacagtc
     1681 gatatgggaa aaccggtgtt ggaagctgaa aaaattccta ctacgcttcc gggaaaacag
     1741 attgttaatt tccctgtttc ctttgacggc atcaattacg ctattacttg tgtttcaatg
     1801 ggcaatccac atactgtaat attcacggag aatattgcca atatggatat cgaaaagatg
     1861 ggaaaaaaaa ttgagaatgc tcccattttt cctcgtcgta ccaatgtgga atttatcgag
     1921 gtgatcgaca aaagccgcat agaaatgcgt gtctgggaaa gaggatcggg tgaaacaatg
     1981 gcttgtggta caggtgcctg tgcatcggtt gtggccggtg tgctaaacgg gcttgtgtcg
     2041 cgtaaggcta ctgtggaact tttaggtgga aatttggaaa tagaatggaa cgaaaacgat
     2101 gaacatgttt atttgaccgg gccggctgaa acagtttttg aaggcgaaat ttcctaagtt
     2161 ttcatttttt tctacaaaac atattgattt tcaaaataaa acgtattttt tatcatcatt
     2221 aatcgaatca ttaaaatggc ttatatcaac gaaaatttta taaaaattcc tccaacctat
     2281 ttattttccg aaattgccaa aagggtggaa caacacaagg cagaaaatcc ttcagctaaa
     2341 attatccgta tgggaattgg agatgttaca cttcctttgc ccgacgcttc ggtagatgct
     2401 atgatcaaag ctgcggaaga acaacgtcat gcggaaactt tcagaggtta tgggccggaa
     2461 cagggttatg aatttttaag acaggccatt gtggaaaatg attttcgggc aagaggaata
     2521 aaactcgatg ttgatgaggt ttttgtcagc gatggagcaa aaagcgatac gggcaatatt
     2581 ggcgatattt tcggtatcga caattgtgtg gccattaccg atccgagtta tcccgtatat
     2641 gtggacacca acgctatggc cggaagagcc ggcgaaccaa acgaatttaa cgaatggaac
     2701 ggactgacct atcttccctg caacgaagct aacaactttg ttcctcagct tccggataaa
     2761 aaagtagata tgatttatct ctgctacccc aataatccga ccggcaccac acttagcaaa
     2821 cagcaacttc aggtttgggt ggagtatgca aaagccaatc gttcggtgat attgtttgat
     2881 gcagcttatg aagcttttat tactgaagaa aatgttccac acagcattta tgaaatagaa
     2941 ggggccaaag aagttgccat cgaatttcga agtttttcta aaactgccgg atttaccgga
     3001 acgcgttgtg gttacacggt tgttcccaaa gagttggttg gttatacgca tcagggagaa
     3061 gcggttgctt tgaacaaatt gtggctccga cgccaatcta ccaaatttaa cggcacttcc
     3121 tacattgtac aaaggggcgc tgaagctact tttacgcccg aaggcagaaa acaggtgaaa
     3181 caacttattg aatattatct gggaaacgcc cgcatcattc gtgaaggcgt tgccaaattg
     3241 ggtttaaagg ttttcggcgg tgtcaatgct ccctatgttt gggtaaaggc acccgacaat
     3301 atgaaaagtt gggaatattt tgactttctg ctgaaacaaa aaaatatcgt tacaactccc
     3361 ggagcaggtt ttggtcgcag tggggaagga tatatccgtt tctcggcatt tggcagcaga
     3421 gaaaacacca ttgaagcgat ggaaaggcta ataaaataat tgatagtact ttcttcataa
     3481 gttattgctt atcaaattta caagacaacc aaaaatgatt ttttcaattt tggcgtgatt
     3541 gattacattt gtctttgttt tttctaattg aaaagagaac ctgttttggc tgctcaatac
     3601 cgatttcgat atcgaaaata agctttcaat cgcaacacaa atgaatactt cttcttcgca
     3661 aagaaaaatt tccgaccccg aactgggtga tatttatttc aacagaagtc aacgggctac
     3721 gcgcataaac attcaaattt tagaagataa attgaaagtt accctgccca tgcgggcaag
     3781 cgaaaaagat gcaatgagat ttatcgactc ggttcgtgaa aaaattttaa aaaaacgagc
     3841 tcaagtaaag gaaaagatac gcaaaaacaa acaaattatt gatgaacaat cccctttcag
     3901 ggcgctgact tttgttactg atatcaaacc cacgcatcga gaagatatct attgttgtct
     3961 gaaaaatcag gttctctcca ttgaaattcc ttcggaagtt gattgcaggg aagaacaaat
     4021 gcaaaattat ttacacagga ttattgctcg ttatctcagg ttagaagcga aaagagtttt
     4081 accccaacgc accagactgt tggcagacaa atttggtttc aggttttcca ctgtcaagat
     4141 tcaatcaagt cgtacccgct ggggaagttg ctccgcaaag gggaacataa atttgtctct
     4201 ttatctgatg gctttaccgg cgcacttaat cgactatgtt attttacatg aactttgtca
     4261 taccagggaa cttaatcaca gtacggactt ttggcgctgg ttgaatcgcg ttaccgacaa
     4321 ccaaagcgac agattgaaag aagaattgaa aaatttcaac ttcaattggg actaggcgct
     4381 aacgttttca accgaaattt gttttatcaa taataaattc gtgaatatga actggaagca
     4441 attgattacc accaaaagac tcgggatgga agcctatcat gaaaagcgtc aggataaccg
     4501 cagcgaattt cagcgcgatt acgaccgatt gattttttct cctccatttc gtcgtttaca
     4561 gaacaaaaca caggtttttc cacttcccgg aagcgttttc gttcataatc ggcttacgca
     4621 cagcttggaa gtttccagtg tagggcgctc gctggggatc aatgtggcac gtcaaataaa
     4681 agagaaaaat aatttacccg atgcttttat tgatgaagtc gggagcattg tttcggcagc
     4741 ttgtctggct cacgacatgg gaaatccgcc tttcggacat tccggcgaaa aagccatagc
     4801 aacctatttc tcggaaggag aaggacaaat tttgcaggag aaaatgttgc ccgaagaatg
     4861 gtcggactta ataagttttg aaggcaatgc caacactttc cgcgtgctta cccatcaatt
     4921 cagaggcaga cgtccgggtg gtttcgttct cacatacagc acactggctt ccatagtaaa
     4981 atatccctat gcttcagttt tttcggatcc gcaacgacaa aaatttggtt tttttcattc
     5041 tgaaaaatcc gattatgaaa aaattgctca cgaactcggt attcctcatt cgttttcaca
     5101 accggacaaa tatgttcgtt atccgttggt ttatctggta gaagctgctg acgacatttg
     5161 ctaccaaata atggatattg aagatgcgca caaactgaaa attctttcca ccgacgaaac
     5221 aaaagctctt ttcctttcct attttgaagg gaagaaactc gaaagaaaac tgcaaactat
     5281 tgctatggtt gatgatgtca acgaacaaat tgcctatttg cgttcttcgg ttattggaca
     5341 gctcattgac aactgttctt ctgtttttct ggaaaatgaa cctcaaattc tggaagggag
     5401 tttcagttcc acattaataa aactgctgcc tcccgtgtcg ctgaaagctt accaaaactg
     5461 ttcaaaaacg gcttttgaga aaatttactg ttcttcggaa gtgcttgatg ttgaacttgc
     5521 aggttataaa atcattttta ctttactcga aaatttggta aaagcggtgc tgagtcccga
     5581 aaaagcatat tctcagcaat tgctcatgcg cattcccaaa cagtatgaga ttgatcagcc
     5641 gacaacttac ggaaaagtaa tggccattct ggattatatt tccggaatga ccgatgttta
     5701 tgcactggat ctttacagaa aaattacggg aatgaacatc ccaacgctct gaacacagaa
     5761 agactacctt tatttttatg gtcaatagcc caccgtttga gcaacaataa agcgataatg
     5821 actgtccaaa cttagcagag aaagcagttc atctgccttt gctccggcac gaaccacaca
     5881 cctcaatcct tcagaagcac aatataaata aacgttttgg gtacaatgtc cggcatcgag
     5941 cgaaaccatt cgtatgctat tcgcgtcagg ttctttctgc gtcaaattgt atttgttgaa
     6001 gtcggcaata aagatcaaat tcatcggagc cgtgtaaaca aaatcctgca agcctgccaa
     6061 ttttctgtaa tctcctttta caacaggcac aagtcgatgt gtttgtggtt cgtatcgata
     6121 aattccattg ggaagtactg cataaacttg cagtggataa agagctcttg ccgagggaac
     6181 cgttcttttt ccatcaggac gattgatgcc ataagctgcc caaagtattt cggacaagtg
     6241 cttcaatgac aatggcgttt gcttaaattc ccgatctgac ttgcgatgtt gaaaagtttg
     6301 catcaaagat tttccaccca ttgtatcagg tgcaagcaat tgaatagtgt caaatgtttt
     6361 taagtccata ggcagagtgt tttgagattt ttccacgcct tttttgtttt gccccgattg
     6421 gaacgaaaag aatgtaaaaa ccatcatgat ggacagtaaa aaaagttttt tcataacatt
     6481 catttttttt gatcaataaa ttcaatcagt aaatgcaatt ttttagggga ataaaaaatc
     6541 aaggaaaatc atttcaagat aaaaaagaag aagatttatt ccggtttgcc cacttagatt
     6601 atggttttct atggtttctt ttcactaaaa aattacaaac aaagaaacat ttaaccgtag
     6661 aacaaagtta caaaatttgg gtaaaaatac aacaaagatt caaataaaaa ggcatagggt
     6721 tggctatcaa aaaagacaaa tcagttgttt agtgcaaaag gctctgaaat tgcaataatc
     6781 taaaatttcc aaaaggaaaa aaattgtttt taaaaaaggt acgcgttgtt ttccaaacta
     6841 tggagggctc ttcgaagttt gatttctatt tgctttgaaa aaatggttaa tatttaattc
     6901 ggatgaaact ggtaaaaaga gtaaattaaa agaaaaaaaa ttttattgtc aaaaactttt
     6961 aaatatctat gttttttcca ttgaaattat tctttgaaaa acaattatat tgttaacttt
     7021 gcagaaaata ttaaataagg aaatatttcg ctaattgacc tttctgtaat tacaattttt
     7081 aattttattt tattgattat caataaaata atttaacaca caactatatt tttcatttta
     7141 tgaaaccaaa aaaaattgaa cacattggca ttgccgtaaa aagtttggaa actgccattc
     7201 ctttttttga aagcgtattg ggacttacat gttatgctat agaggaagtg aaagaccaaa
     7261 aagttaaaac agcttttttt aaggttggag acactaaaat tgagctgttg gagtcaactg
     7321 atcctgaagg accaattggt aaatttattg aaaaacgcgg agaaggagtt catcatattg
     7381 ctttagcagt agaaaatgtt cagcaagcct taaatgaagc atccgataaa ggagttatgt
     7441 tgatagacaa acaaccacgc aaaggtgccg aaggtttaaa tattgctttt atacatccaa
     7501 aatctacttt tggaattttg actgagttgt gtgaaaatcc caatataaat tgttgattta
     7561 tttttaaaaa atctttaaga taaaaagaaa aaatgaataa tctggaaaaa ttagaacaat
     7621 taatcagttt acgtgcaaaa gccaagctgg gtggaggaga aaagaaaatt gaatcacagc
     7681 acaaaaaagg caaatataca gcgcgagaaa gaatcgaaat gttgcttgat gatggaagtt
     7741 ttgaagagct tgatatgttt gttacacatc gttgtaccaa ttttgggatg gaaaacgaaa
     7801 aatacttggg agatggtgta gtaaccggac acggtacaat tgatggacga acagtgtatg
     7861 tttatgccca agattttacg gtttttggtg gttcactttc ggaaatgatg gcacaaaaaa
     7921 tttgcaaagt gatggacatg gctatgaaga tgggtgctcc aattatcgga atcaatgatt
     7981 cgggaggcgc acgaattcag gaaggggtaa cttcactggc cggatatgcc gaaatttttg
     8041 aacgaaacat tcttgcttca ggtcttgtac cacaaatttc agctattttt ggcccttgcg
     8101 ccggaggagc cgtttattca ccggcactta ctgattttat tttgatgaca gaggaaaaca
     8161 gttacatgtt tgttaccggt cccaaagttg ttaaatctgt tacgggtgaa gatattacaa
     8221 ctgaggatct tggaggcgcc aatgttcatt cttcaaagtc gggagtgtct cattttaagg
     8281 ttgaaagcga agaggaaggg ttgctgatga tacgtaaatt attatcgtat ttacctcaaa
     8341 ataatcttga agaacctcca ttgattcaaa actatgatcc aatcgatcgt catgaagaca
     8401 aactgaatac catcgtacct gacaatccga atttgcctta cgacatgaaa gaaattattc
     8461 attcattggt agataacgga gaatt
//
LOCUS       DAOSCH010000086         8468 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5460_length_8468_cov_5.746226, whole genome shotgun sequence.
ACCESSION   DAOSCH010000086 DAOSCH010000000
VERSION     DAOSCH010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8468
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5460_length_8468_cov_5.746226"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(834..2723)
                     /locus_tag="PLZ54_06945"
     CDS             complement(834..2723)
                     /locus_tag="PLZ54_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOJ66699.1"
                     /translation="MANYLQVENLTKSFGDRLLFENISFTVTENQRVALIAKNGTGKT
                     TLLNILTGREDYDSGNIVYRSDLRIGYLPQNPDFRKDLTVMDACLTSDSEIVRTIAQY
                     ELCLSSHNEQKMNEIVARMDALRAWDYELRVKQILTKLKITDFDQKIGELSGGQLKRV
                     ALAQVLITEPDLLLLDEPTNHLDLEMVEWLEDFLKRSSMSLLMVTHDRYFLDRICTDI
                     LEIDFQKLFHYKGNYTYYLEKRQERLDAFNAETDRTRQLYRTELEWMHRQPQARGTKA
                     KSRIDRFHEIEQRARQRFDDKVIKLNVKASYLGSKIFEAKNVSKSYGELKILENFNYV
                     FSRYEKLGITGNNGTGKTTFLKMLLGEVQPDSGTFDIGETVVFGYYSQEGLNFDEQMK
                     VIDVVQNIAEVIDLGNGKKMSASQFLQYFLFSPESQYDYVYKLSGGERRRLYLCTILM
                     KNPNFLILDEPTNDLDIVTLNVLEDYLQNFKGCLIVVSHDRYFMDKVVDHLLVFKGNG
                     ILKDFPGNYTDYREWNDLMEEQERENEKQLKQNSFSGLSQKNETNEKNRPKKLTYKEQ
                     KELETLEKEIPILENEKREIEIRLGSGEPNSEEMVQLSHRFSELMQLIDEKSLRWLEL
                     MENID"
     gene            2856..3539
                     /gene="mnmD"
                     /locus_tag="PLZ54_06950"
     CDS             2856..3539
                     /gene="mnmD"
                     /locus_tag="PLZ54_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOJ66700.1"
                     /translation="MDSELILTQDGSHTLFVPDIDECYHSTFGAIGESTLIFIQSGFN
                     CCEKDKINVLEVGFGTGLNAFLTLLEAENRNKKVFYMAFELFPLSVEKAKQLNYPEKV
                     SPEKRFFFEKLHTSIWGEEVPITPFFKLLKINADFIVSSLPSVYDLIYFDAFSPEKQP
                     EMWSQKIFRKIFDHCNENAILTTYCVKGVVRRALQNTGFVVEKLPGPPGKREFLRAVK
                     PSNADFTEK"
     gene            3645..3914
                     /gene="rpsO"
                     /locus_tag="PLZ54_06955"
     CDS             3645..3914
                     /gene="rpsO"
                     /locus_tag="PLZ54_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007763600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOJ66701.1"
                     /translation="MYLTQEKKKEIFGQYGKSNTDTGSPEAQIALFSYRINHLTEHLK
                     SNKKDYSTERALIKLVGKRRRLLDYLKEKDINRYREIIKELGIRK"
     gene            4002..5264
                     /locus_tag="PLZ54_06960"
     CDS             4002..5264
                     /locus_tag="PLZ54_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOJ66702.1"
                     /translation="MRYLYSIGIHIYRFLIFLASFFNPKARELYKGQKNALRYLKNNI
                     EENARYVWFHAASLGEFEQGRPIMEALKRQHPDLKILLTFYSPSGYNVRKDYRGADVV
                     AYLPPDTRRSARAFVKTVKPLKAIFIKYEFWPNFLLTLRAEGVPTYAVSAIFRPNQIF
                     FKWYGKWYLQLLTTFRRLFVQDQDSAVLLEKHGIHQVSVSGDTRFDRVFALFSEAKKL
                     PLVEAFVAGKPVIVAGSTWPEDEDLLVKYLKKHPKIKLILVPHEIHKNHLEQISKLLD
                     GKFIRYTEASDDNIADYNCLVVDVVGILSSIYQYAKVAYVGGGFGVGIHNILEAAVWN
                     VPVVFGPNFQKFKEARDLIQLKSAFSINDYISLEENLDKLLCDEKAGQMAGNYVRENI
                     GATELICRNLKFPEPAKNANEEKAQNKS"
     gene            5305..6669
                     /gene="hisS"
                     /locus_tag="PLZ54_06965"
     CDS             5305..6669
                     /gene="hisS"
                     /locus_tag="PLZ54_06965"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOJ66703.1"
                     /translation="MNQKPSLPKGTRDFSPEEMTKRNYIFDTIKSVFRLYGFQQIETP
                     AMENLSTLLGKYGEEGDKLLFKVLNSGDFLSGISDAELLERNAARLSGKICEKGLRYD
                     LTVPFARFVVQNRDKISFPFKRYQIQPVWRADRPQKGRYREFYQCDADIIGSNSLFNE
                     LELIQIIEEVFCRLKINVCIKINNRKILSGIAEYIGEPEKLTDITTAIDKIDKIGIEN
                     VNEELLSKGINREALGKLQPLLQLKGSNQEKLAQLKALLFESAAGMKGIAELETILDL
                     CEQANVCVQIELDLTLARGLNYYTGAILEVKALDVEIGSISGGGRYDNLTGVFGWEGV
                     SGVGVSFGADRIYDVLYQLQLFPEISAEQTKILFVVFGEKELKFSLQWAQALRKENIN
                     TEIYAEPSKLKKQLGYADNKKIPFVAIVGENEMQEGKITFKNMATGEQQMISLEELIQ
                     RLKS"
     gene            6825..7016
                     /gene="rpsU"
                     /locus_tag="PLZ54_06970"
     CDS             6825..7016
                     /gene="rpsU"
                     /locus_tag="PLZ54_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOJ66704.1"
                     /translation="MIVVPVKEGENIDKALKKFKRKFEKTGVVKELRRRQCFDKPSVI
                     KRNQKIRAVYLQKLQQEEK"
     gene            7083..7970
                     /locus_tag="PLZ54_06975"
     CDS             7083..7970
                     /locus_tag="PLZ54_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ66705.1"
                     /translation="MVIMNEFLEYLRYERNYSPYTVLSYQTDLSQFIEFMHETPETFT
                     PSQVTPQQIQQWIMLLMSEKKSARTLSRKISTLKSFWHFLLSRGYVAKNPVSNIILPK
                     KSKPLPAFFKKEEMEAALDETFLTDDFEKLRNHLMLELFYFSGIRRSELISLRDSDVD
                     FISRQLSVIGKRNKERIIPLNKKLCQDIRRYIQVRNKTIENKSQWLFVRKNGDKLYPK
                     LVYNLVHQTMSAYSSQSKRSPHVIRHSFATHLLNEGADINAVKELLGHSSLAATQVYT
                     HTSFEELYKIYKQSHPRGK"
     gene            7985..8284
                     /gene="raiA"
                     /locus_tag="PLZ54_06980"
     CDS             7985..8284
                     /gene="raiA"
                     /locus_tag="PLZ54_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOJ66706.1"
                     /translation="MKSRIQAINFDATEELVAYVEKKISKFEKLIDGILNVEVYLKLV
                     KPKISADKEAGIKVSIPNVDFFSSKACETFEEAIDLCVDAIEKQIKKHKEKISKK"
     gene            8394..8467
                     /locus_tag="PLZ54_06985"
     tRNA            8394..8467
                     /locus_tag="PLZ54_06985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8428..8430,aa:Thr,seq:tgt)
BASE COUNT         2688 a         1532 c         1595 g         2653 t
ORIGIN      
        1 tatcgatatt aaattctttc tcttcagatt gactaatatc tatgcttaaa cacatgtttt
       61 tgcaggtgag ccgttttttc attttgagaa gagtttattt gattaatttt tgaatgaact
      121 taataaaggg aatcaatttg aataagacgc tttcgattgt aatatccacc aaatttacat
      181 cattttggtt aaaaaaagaa aggacttcca aattttccaa atgaaaaaaa tgaaagtcct
      241 ctgcttaacc aaccattgca aaaaaactaa ttgcaatcat tctcctaaca acacaacttt
      301 ttttacctct tattttatct cactaaagtt tcgcagttca gaaagtgatg aaaataaaga
      361 ggaaaaaaga gaaaaatgag taaaaaagca gcgtatttta tgaaatatat cattaaaaat
      421 gagtatatta gctgtccaat tacaaaaaca gaaaaatatg ctaccggaca aaattaagga
      481 aaaaatttct gaaacaggaa cagatttcaa tgaaacccac atgtattaca tacacaaaca
      541 cgaatgaata taacatttag acatgtgggt tccatcagac cattaaaaaa ttaaattata
      601 ttctgatgaa aataaattag agagcatacc tattttgaaa gctttcaagg atgcttgctc
      661 ctccgctcag ggaagcagtt tgacgaaaat ggaattattt atatattgat aatcaggcag
      721 agaataatta aatcgcactt ctttattttc tgtctttcaa atcattaaaa cgaagtgcga
      781 aactttagca atattaaatt caattttgaa ttattctttt tctcgtaatc ttttcagtcg
      841 atattttcca taagttcgag ccagcgtaaa gatttttcat cgatcaattg cattagttcc
      901 gaaaaccgat gggataattg taccatttct tcgctgttcg gttctcccga tcccagccgg
      961 atttctattt ctcttttctc attttctaaa atcgggattt ctttttccaa agtctccaat
     1021 tctttttgtt ccttgtaggt taatttcttt ggccgatttt tttcattggt ttcgtttttt
     1081 tgagacagac cactgaacga attttgcttt aactgtttct cgttttctct ttcctgctct
     1141 tccatcaaat cgttccattc ccggtaatcg gtataatttc ccggaaaatc cttcagaatg
     1201 ccatttcctt taaataccag caaatgatct accactttgt ccatgaaata acggtcgtgg
     1261 cttaccacaa tcaagcagcc cttaaaattc tgcaaataat cttccaatac attcagcgta
     1321 acaatatcga ggtcgtttgt gggctcgtcc agaattaaaa aattgggatt tttcatcaag
     1381 atggtacaga gatacaagcg ccttctttca cctccactaa gtttgtaaac ataatcgtac
     1441 tgactttcgg gagaaaagag gaagtactgt aagaactgag atgccgacat ttttttaccg
     1501 ttacccaaat caatcacttc ggcaatattc tgaaccacat caatgacttt catttgttcg
     1561 tcaaaattca atccttcctg actatagtaa ccaaaaacga cagtttctcc gatatcgaaa
     1621 gttccactgt cgggctgaac ttctcccagc agcattttca aaaaagttgt ttttcccgtt
     1681 ccgttgtttc ctgtaattcc cagtttttca taacgggaaa acacataatt gaaattctcc
     1741 agtattttaa gttcgccata agattttgaa acattttttg cttcgaaaat ttttgaaccc
     1801 aagtagctgg ctttaacatt gagttttatt actttgtcat caaaacgttg acgtgcgcgc
     1861 tgttcaatct catgaaaacg atcgatgcgc gattttgcct ttgttccacg agcttgaggt
     1921 tggcgatgca tccattccaa ttctgttcga tacagttgtc gggttcggtc ggtttcggca
     1981 ttaaaagcat cgagcctttc ctgtcgtttt tccaaatagt aagtataatt tcctttgtaa
     2041 tgaaagagtt tttgaaaatc tatttccaaa atgtcggtgc aaattcggtc gagaaaataa
     2101 cgatcgtggg taaccataag tagggacatg gaggagcgtt tcaaaaaatc ttcgagccat
     2161 tctaccattt ccaaatccaa gtggttggtc ggttcatcga gcaaaagcaa gtccggctcc
     2221 gtaatcaata cttgagccag agccactctt ttaagttgtc caccggaaag ttcaccaatt
     2281 ttttggtcaa aatcggttat tttcaacttt gtgagaattt gtttgacacg tagttcgtag
     2341 tcccaagctc tcaaagcatc catgcgcgca acaatctcat tcatcttctg ctcattgtgt
     2401 gaagaaaggc aaagttcata ttgtgctatt gttctcacta tttcgctgtc ggaagttaag
     2461 caggcatcca tcacggttaa gtctttccga aaatccggat tttgaggtaa atatcctatg
     2521 cgcaaatcgc ttcggtaaac aatatttccg ctgtcgtaat cttcacgacc ggtaaggata
     2581 ttcaataagg ttgtttttcc tgttccattt ttagctatca gtgcaacccg ctgattctcg
     2641 gtaacagtaa aagaaatatt ttcaaaaagc agtcgatcgc caaatgattt ggtgagattt
     2701 tccacttgca gatagttcgc catattcaat aatttgatac aaagataaca ctttccgagc
     2761 attgggtcat aaatgtttgc catttcgcgt gtttcactcc tcgtgaaaaa ttattaattt
     2821 tactgccaat aaattgtaaa ttctgttttc aataaatgga tagcgaactc attttaacac
     2881 aagatggttc acatacgttg tttgttcccg atatagatga atgttatcat tctactttcg
     2941 gtgcgatagg tgagtccacc ctgattttta tacaaagtgg tttcaactgt tgtgagaagg
     3001 ataaaatcaa tgtgcttgaa gttggttttg gcacgggtct gaatgctttt ctgacattgt
     3061 tggaagcgga aaatcgaaac aaaaaagttt tttatatggc gtttgaactt tttccattgt
     3121 cggttgagaa agccaaacaa ctgaattatc ccgaaaaagt ttcaccggaa aaacgtttct
     3181 ttttcgaaaa actgcatact tctatttggg gtgaagaagt acccattact ccctttttta
     3241 agttgttgaa gattaacgct gattttatcg tttcctcttt gccgtcagtt tacgacctta
     3301 tctattttga tgctttttcg ccggagaaac agcctgaaat gtggtcgcag aaaatattca
     3361 ggaaaatttt tgaccactgc aacgaaaatg ccatattgac cacctattgt gtaaaaggtg
     3421 tggtcagaag agcactacaa aacacgggat ttgttgtgga aaaacttccc gggccgccgg
     3481 ggaaacgtga atttcttcgg gctgtgaagc cttcaaatgc tgactttaca gaaaagtaac
     3541 gaattaattc tcaatttttt caaaagaatt tgaaaaaaga gtgttcgtta attaaaagaa
     3601 aatttatacc tttgcagttc attattaaac ataaaacttt cattatgtat ttaactcaag
     3661 aaaagaagaa agaaatcttt ggtcaatacg ggaagtctaa tactgatact ggttcgcctg
     3721 aagcccagat agcattgttt tcttaccgta tcaaccattt gactgagcat ttgaagtcaa
     3781 acaaaaaaga ttatagtact gaacgcgctt tgattaaatt ggtaggtaaa cgtcgtcgct
     3841 tgttggacta cctgaaggaa aaagacatca accgttaccg cgaaattatc aaagagttgg
     3901 gcattcgtaa gtaattcagg catttgtcaa cttttaaaag gcgtttcaaa actacttgaa
     3961 atgccttttt aatttagaaa cctctttccg caaattaaag gatgcgttat ttatattcca
     4021 ttggtataca tatttacaga tttcttattt ttcttgcttc atttttcaat cccaaagctc
     4081 gtgaacttta caaagggcag aaaaatgcgc tccgatattt aaaaaacaat attgaagaaa
     4141 atgcccgtta tgtgtggttt catgctgcct ctttgggcga atttgagcaa ggacgaccga
     4201 taatggaagc attgaaacgc caacatcccg atttaaaaat tctcctcact ttttattctc
     4261 cttcaggtta taatgtcaga aaagattatc gaggtgccga tgtggtagca tacttgccac
     4321 ctgatacgcg tcgttcggca cgtgcatttg taaaaacggt gaaaccactg aaagccattt
     4381 ttattaaata tgaattttgg ccaaattttt tgttgacgct tcgggcagaa ggtgttccca
     4441 cttatgctgt ttcggcaatt tttcgcccca atcagatctt cttcaaatgg tacggaaaat
     4501 ggtacttgca attgttgacc actttccgac gtttgtttgt tcaggatcaa gattcagcag
     4561 ttttacttga aaaacacggc attcatcaag tcagcgtcag tggtgatacg cgtttcgaca
     4621 gagtttttgc tcttttttcc gaagcaaaaa agcttccttt agttgaagcg tttgttgctg
     4681 gaaaaccggt aattgtggca ggtagtactt ggcccgaaga cgaagatttg ttggtgaaat
     4741 atctgaaaaa acatccgaaa atcaaattga tattggttcc ccatgaaatt cataaaaatc
     4801 atttggagca aatctcaaaa cttttggatg gaaaattcat acgctatacc gaagcatccg
     4861 atgataatat tgctgattat aactgtttgg tggtcgatgt agtgggaata ctttcttcca
     4921 tttatcagta tgcaaaagtg gcttatgtag gtgggggctt tggtgtaggt attcacaata
     4981 ttcttgaagc agcggtttgg aatgtgccgg ttgtatttgg cccaaatttt caaaaattca
     5041 aggaagctcg cgatctgatt caactgaaaa gtgctttttc catcaatgat tatatttcgc
     5101 tggaagaaaa tttggataag ttgctttgcg acgaaaaggc aggacagatg gccggcaatt
     5161 atgttagaga aaatattggt gctacagaat tgatttgtag gaatttaaaa tttcccgagc
     5221 cggccaaaaa tgcaaatgaa gaaaaagcac aaaacaaatc ataatttatt tttcacagtt
     5281 tactaaagaa aattttttta aacgatgaac cagaaacctt ccctcccaaa aggaacacgc
     5341 gatttctcgc ccgaagaaat gacaaaacgc aattacattt ttgataccat caaaagtgtt
     5401 tttcgattgt atggatttca gcaaatagaa actcctgcta tggaaaactt atccacatta
     5461 ctaggaaaat atggagaaga aggcgataaa ttgttgttca aagttctcaa ttctggagat
     5521 tttctttcgg gaatttccga tgcagaactg ttggaaagaa atgctgcccg tctgtcgggt
     5581 aaaatttgcg aaaaagggct tcgctatgat ctgactgttc cttttgcccg ttttgtggtg
     5641 caaaatcggg ataaaatttc gttcccgttc aaaaggtacc aaatccagcc cgtttggcgt
     5701 gccgatcgtc cgcaaaaagg gcgttatcgt gaattctatc aatgcgatgc agatattatc
     5761 gggagcaatt ctctttttaa tgaattggaa ctgatacaaa tcatagaaga agttttctgc
     5821 cggttgaaaa tcaatgtctg cattaaaatc aacaacagaa aaattctttc gggcattgcc
     5881 gaatatattg gagaacctga aaaactgacg gatataacta cggctatcga caaaatagac
     5941 aagattggta tcgagaatgt gaatgaagaa ttgctttcaa aaggaatcaa tcgcgaagct
     6001 ctcggcaaat tgcagccttt gttgcaattg aaaggctcaa atcaggaaaa acttgctcag
     6061 ctgaaagctt tactttttga atcggctgca ggaatgaaag gaattgccga gttggaaaca
     6121 attttggatc tttgtgaaca agcaaatgtt tgtgtccaga tagagctcga tttgacgttg
     6181 gctcgtggtt taaattatta tacaggagct attttggaag tgaaggctct cgatgtggaa
     6241 attggaagca tttcgggtgg aggtcgttac gataatttga caggtgtttt cggctgggaa
     6301 ggagtttcgg gtgtaggagt ttcctttggt gccgacagga tttatgatgt actttaccag
     6361 ttacagcttt tccccgaaat ttcggccgaa caaaccaaaa ttctgtttgt ggtttttgga
     6421 gaaaaggaat tgaagttcag cttgcaatgg gcacaggcat tacgcaagga aaatatcaat
     6481 accgaaattt atgccgaacc ctccaaactg aaaaaacagc tcggttatgc cgacaacaaa
     6541 aaaattcctt ttgtggcaat agttggagaa aacgagatgc aagagggaaa aatcacattc
     6601 aaaaatatgg ctaccggtga gcaacaaatg atttcgttgg aagagctcat tcagcggttg
     6661 aaaagttgaa aattcgaatt tttccccata cagagagaaa tttgaacttt caaaattttg
     6721 acattaaaaa aatatttgat ttttcagaac aaatgattaa ctttgcacac catttttaga
     6781 aaccataaaa ttactattca aaaccaaatt tataattaaa aattatgatc gtagttccag
     6841 ttaaagaagg agaaaacatt gacaaagctt tgaaaaaatt caaacgcaaa tttgagaaaa
     6901 caggtgttgt taaggaactt cgccgtcgtc agtgtttcga taaaccttct gttatcaaac
     6961 ggaatcagaa aatccgtgca gtttatctgc aaaaattaca gcaggaagaa aaatagaaaa
     7021 aatccgttaa aaagtttgct tttccggttt tttcattcta ttttcgttgc ataattgaac
     7081 ttatggtcat aatgaacgaa tttttagaat atttacggta tgagaggaat tattctcctt
     7141 ataccgtttt atcgtatcag accgaccttt cgcaattcat tgaatttatg cacgaaacgc
     7201 ctgagacttt tacaccttct caggttactc ctcagcaaat tcagcaatgg attatgctgt
     7261 tgatgtccga aaagaaatcg gcacgcactc tttcgcgtaa aatctccacc ctaaaatctt
     7321 tctggcattt tttgctttcg agaggttatg tagcaaaaaa tccggtttca aacattattt
     7381 taccaaaaaa gtcaaaaccg cttccggctt ttttcaaaaa agaagaaatg gaagctgctt
     7441 tagatgaaac ttttttgact gacgattttg aaaaacttcg aaatcacttg atgctggagc
     7501 ttttttattt ttcgggaatt cgccgatcag agctcatttc acttcgcgat tccgatgtcg
     7561 attttatcag tcggcaacta agcgttatcg gaaaacggaa taaagaacgc attataccgt
     7621 taaataaaaa attgtgccaa gatattcggc gttatattca ggtaagaaac aaaactattg
     7681 aaaacaaaag tcagtggctt tttgttagaa agaatggaga caaactttat cctaaacttg
     7741 tttataacct ggtacaccaa accatgtctg catatagcag ccagtccaaa cggagtccgc
     7801 atgtcattcg tcatagcttt gcaacccact tgctgaacga aggcgcagac atcaatgcag
     7861 taaaagaact cttgggacat agcagtttgg ctgctacaca agtgtatacc cataccagtt
     7921 ttgaggaact ttataaaatt tacaaacaat ctcatccacg tggaaaataa aaaaggaggt
     7981 cattatgaaa tcaagaattc aagccattaa ctttgacgcg acagaagaat tggtagcgta
     8041 tgtagaaaag aaaatctcaa aatttgagaa actcattgat ggaattttaa acgtggaagt
     8101 ctatttgaag ttggttaagc cgaaaatttc ggccgacaag gaagccggga taaaagtatc
     8161 tattccaaat gtagatttct tttcctctaa ggcctgcgaa acttttgagg aagcaataga
     8221 tctttgtgtt gatgccatcg aaaaacaaat taaaaagcac aaggaaaaaa tttctaaaaa
     8281 ataattctca aaaaggtttt gaaattaaaa ataattcagt aaatttgcaa gccgtttaag
     8341 taggaatatt taagcggctt tttaaacacg aaaatggtgt tttttagaca attgcctctt
     8401 tagctcagct ggccagagca cgtgatttgt aatctcgggg tcgttggttc gaatccgaca
     8461 agaggctc
//
LOCUS       DAOSCH010000087         8396 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5533_length_8396_cov_8.891020, whole genome shotgun sequence.
ACCESSION   DAOSCH010000087 DAOSCH010000000
VERSION     DAOSCH010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5533_length_8396_cov_8.891020"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1666)
                     /locus_tag="PLZ54_06990"
     CDS             complement(14..1666)
                     /locus_tag="PLZ54_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ66707.1"
                     /translation="MLQIFAQILAFPGAEGDGMYTTGGRGGKILYVTKLTDDGTTGTL
                     RWAINQSGARIIMFKVAGIIPLTSELRIKNGNLTIAGQSAPGDGICLKNYPVVIQASN
                     VIIRFIRFRMGDEAAAGNTSYSWDGADAIWGRNQSNIIIDHCSMSWSIDECSSFYDNK
                     NFTLQWCVIAESLRKSVHGKGTHGYAGIWGGAPATFHHNLLAFHDSRNPRFCGSRFSN
                     SPSTERVNFTNNVIYNWGSNSGYGGEGGYYNMMNNYYRSGIARSSGSTSYRIFSPNAD
                     DGTNSQPKGVWGHFHLSGNIMINTPSVTDNNWLGFQPVGTYSDTIKSEVPFDFPPYLR
                     LQRADSAYLSVTGFAGASLKRDAVDTRIIDNVLNGTVTVTQGSNGSTGGFIDSQADVG
                     GWPEYTYDPATVPTDTDGDGMPDDWETAKGLNPNDASDGSTKTIDGQYTNVEVYLNEL
                     AAPVVNRQYLDANTAVNEVYANSSLRIYPNPFKSGNLNIKAAEKINKVEFYTLSGAKI
                     SEKETNGTEVSFTMQQLNAGIYIVKVKMEDGIILPTLLIVEP"
     gene            complement(1725..4940)
                     /locus_tag="PLZ54_06995"
     CDS             complement(1725..4940)
                     /locus_tag="PLZ54_06995"
                     /inference="COORDINATES: protein motif:HMM:NF013276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
                     /protein_id="HOJ66708.1"
                     /translation="MKKIFYFLLLFFFVGFSIIKAQTKIDVWDFGAAQFDPTIYNNML
                     DEATINSWYPGVTPGTTGKNLPSSFTVGILSWTSTSSTSDRLRTSNTNLTRYDSNGVP
                     VTVASTSETLTGYLYVNASAATGRYLSLNLNEDDEVTIYAKSQNGTGKMTFEYTSDNS
                     QKDIVSLTSSPEIYKFVAKKAGVYKIYDSVDKPFYYRILRKPATYVSVSGTFDLTNAS
                     DIPSGYTINFTNAAGKVWSVAPDASNNYSINLPAGYTYSLSLSNANGYIISNSDPLSV
                     SGNTTYNPVILKVTLYTLSGNITGLPSEKLAKLGLIFTPSDTRIYVPKPVVNASAGTY
                     SVQLEANCDYTISATGVNDYYISNNNVNISSDINTDIVFVAKPVYTVLLNINGLDATQ
                     LSKLSVTFSNLNESGYVYSFTDLNNIQLRDGVYSISCSGLDEYPVQLGLTSNLKVNGT
                     ETSKDLSFIPVTDWSFDDATITSGVTTAYKGMLFTGSVYNEKSKGHLVLTGNATAKVP
                     VSPGEKMIVYYYYSAKFTVDGGSEISTNSGSTNRIESHEFVYAESTPGYMTIANVTGT
                     TTYLTEIKMVKSVTYKPIITVGTDKEYQSINDALAAVRCMTRENTDTVKIMIDPGNYE
                     EMLVIDIPNVTLVNASTTPSIALLNKGVDIDENAVRITSYYGHGYNYFSMGTDQKWNA
                     DVLRVNKENGYTTYSNTGSGTTNGSYWNATVVVSASGFRADNIIFENSFNQYISRKES
                     EDVVQEWSTGGKGTRPTTYGSTDVQNKNFVERAAAIAFTASADRAILNKCRVVGRQDS
                     FYGAEGARIAVYKGVLMGATDYLFGGMTLTCYQTDLAMNTSEASSDVAYITAAQQNSA
                     RGFLMYECNITSAIPSVETASLYRSKPGYFGRPWQPTTSEVVFYSTTIETTNNPSFDG
                     QSLIVPAGWNNSLGGTSDKVYEYNTIEKSGVDNSASRVSWSKVLTEPVLSDGTEITTF
                     NFTKGTDNWDPINTLKSNDINASVSLQKKDDFVRIFSVGSKVFISNVKNPSKIKIYSV
                     NGSLSFVKEISGDDEISLDKGLWIVNVVNNESIKTAKVLIK"
     gene            complement(4979..6064)
                     /locus_tag="PLZ54_07000"
     CDS             complement(4979..6064)
                     /locus_tag="PLZ54_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HOJ66709.1"
                     /translation="MADSEMKRNPESWMLDFSKKLKWDYCNGLELQAFLKVWEKTGDE
                     KYFQYAKSYADTIIEENGQIKGYKLEEYNLDRINSGKFLFTLYEKTKDEKYKKAIELL
                     RSQINTQPRTSEGGFWHKKIYPHQMWLDGIYMASPFLAEYAYRFNEPALFDEVAFQIL
                     LIAKHCYDPATGLYYHGWDESRQQKWADPVKGTSPNFWSRSMGWYAMAIVDVLDFLPE
                     NHPKRPEIIKVLTDLCYSLDKYRDPKTGMWFQVTDKINEKGNYVESSGSAMFIYTWVK
                     GAQKGYLPKEFLKKGKKAYKQFVKTFIVENSDGTISLKNACAVAGLGGEKNYRDGSYE
                     YYISEPIRDNDPKAVAPFIMVSVLLNK"
     gene            complement(6190..6981)
                     /locus_tag="PLZ54_07005"
     CDS             complement(6190..6981)
                     /locus_tag="PLZ54_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HOJ66710.1"
                     /translation="MKFRFSAITILGVLMLFFLASAPKPVKVLMAGDSTMADKVLYKT
                     VFDSLTGKSYEEPFLERGWGQLLPELLNDKALVKNYARNGRSTRSFIEEGLWTELLNN
                     TEKGDFVIIQFGHNDESVEKTGRYTNPAQFRLNFVAFVAEVRLKGATPVLCTPVARRN
                     FDNTGRLVPTHGSYPEIIRSVARDYRVPMIDLEKLTSEWLQHEGVEGSKKYFHKIAPG
                     ISRLYPNGLDDNTHFNEAGARTVAEMFVKEVATQKIKGFEKIFKK"
     gene            complement(6978..>8396)
                     /locus_tag="PLZ54_07010"
     CDS             complement(6978..>8396)
                     /locus_tag="PLZ54_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HOJ66711.1"
                     /translation="EAFKQAIDALARKGGGKLIVPAGVWFTGPIELKSNINLHLEKGA
                     LILFSKDFDLYPLVDTYFEGLATKRCQSPISGRNIENVAITGEGSINGSGEAWRPLKK
                     SKVTASEWKKVVNSGGVVKEGNYWFPSEKSYKGYSISDMNVPRAKLTDEEWMEIRDFL
                     RPVMVSFIQCKNVFLQGVLFENSPSWNIHPFMCENVIVDNIIVRNPDYAQNGDGIDVE
                     SCKNVIIVNSYFDVGDDAICIKSGKDEEGRRRAVPTENLIVHNCKVFKAHGGFVVGSE
                     MSGGVRNISVSCCQFLGTDVGLRFKSKRGRGGVVENIFVDGIYMFDILTDSFLFDLYY
                     GGKSATEAMEEGNDNQKDAVLMPVTEETPVFRNIFVKNIVSRNAHRAMFFNGLPEMKI
                     SNIQVENAIITATYGAELNNSKDIVFKNIYVEPTIGPALRLNRIENFKAENFTYPEKL
                     QNAIELKGEENENIIISNVKIK"
BASE COUNT         2505 a         1774 c         1433 g         2684 t
ORIGIN      
        1 attttgaaaa atattatggt tcaactatca aaagtgttgg aagaattatt ccgtcttcca
       61 ttttaacttt tacaatataa attccggcat ttagctgctg catggtaaat gaaacttctg
      121 ttccattcgt ttccttttcg gaaatttttg caccgctcaa ggtatagaat tcaactttat
      181 tgattttttc agcagctttg atattcaaat ttcccgattt gaaaggattg ggataaatgc
      241 gcaaagaaga atttgcatat acttcattta cagcagtatt tgcatccaaa tattgtcgat
      301 ttacgactgg tgcagccaat tcgttcaaat aaacttcaac attagtgtat tgtccgtcaa
      361 tcgtttttgt acttccatct gaagcatcat ttggattcaa gccctttgcc gtttcccaat
      421 cgtcaggcat gccatcgcca tcggtatccg ttggaacagt tgccggatca taagtatatt
      481 cgggccaacc accaacatct gcctgagaat caataaaacc accggtgctt ccattacttc
      541 cttgtgtaac cgtaacagtt ccattcagca cattatcaat tattcgtgta tccactgcat
      601 cgcgctttaa actggcacca gcaaaacccg ttacgctcaa ataagcactg tcggcacgtt
      661 gcagtctcag ataaggcgga aaatcaaaag gaacctctga tttaatcgta tcggaataag
      721 ttccaacagg ttgaaatccc aaccagttat tatcggtaac actgggtgta ttgatcataa
      781 tgtttccgct taaatggaaa tgtccccaaa cgccttttgg ttggctattc gttccgtcat
      841 cagcatttgg actgaaaatt ctgtaacttg tagaaccgct acttcgggct atgccggagc
      901 ggtaataatt attcatcatg ttataatagc ctccttcacc tccatagccg gaattgcttc
      961 cccaattgta aatgacatta tttgtaaaat taactctttc agttgatgga ctatttgaaa
     1021 atctgcttcc acaaaaacgt ggattcctgc tatcgtgaaa agccaataaa ttatgatgaa
     1081 aagtagcagg cgccccgccc caaattccag cataaccatg tgtacctttt ccgtgaacag
     1141 attttctcag actttcagct attacacacc attgaagtgt gaagttcttg ttatcgtaaa
     1201 aagatgaaca ttcatcaata ctccaactca tggaacaatg atcaataatg atattgcttt
     1261 gattacgccc ccaaatggca tcggctccat cccaactgta agaagtgttt cctgcggccg
     1321 cttcatcgcc cattcgaaaa cgtataaaac gaataatcac gttacttgcc tgtattacca
     1381 ccggataatt tttcagacaa attccatcac ctggagcact ttggccggca atggtcagat
     1441 tcccgttttt tatcctgagt tcggaagtaa gcggaataat tccggcgact ttgaacataa
     1501 tgattcttgc acccgactga ttaatagccc agcgcaatgt gcccgttgtc ccgtcatcgg
     1561 tgagtttggt tacataaaga atttttcctc cgcgtccgcc ggtagtgtac atgccgtcgc
     1621 cttcagcacc gggaaaagcc aggatttggg caaatatctg taacaaaaaa atgtttgaaa
     1681 ctaaaacaca caaaattgtt aaaattctat ttttcctcat cattttattt tattaaaact
     1741 tttgctgttt taatgctttc gttattcacc acatttacaa tccacaatcc tttatccaat
     1801 gaaatttcat catctccgga tatttcctta acaaaagata aagagccatt tacggaataa
     1861 atttttattt tggaaggatt ttttacattt gaaataaaga ctttgctccc aactgaaaat
     1921 atccgaacaa aatcatcttt cttttgcagt gaaacacttg cattaatatc attagatttt
     1981 aaagtgttta ttggatccca attatcagtt ccttttgtaa aattgaaagt agtaatttct
     2041 gttccgtcgc ttaaaaccgg ttctgtaagc acttttgacc aagaaacgcg agaagcagaa
     2101 ttatcaactc cagatttttc tatggtgttg tactcgtaaa ccttatcgga agttccaccc
     2161 aaagaattat tccatccggc cgggacaatc aaggattgcc cgtcaaaact cggattattt
     2221 gtagtttcaa tggttgtaga gtaaaatacc acttcgctgg tagttggttg ccacggacgg
     2281 ccaaagtatc cgggtttgga gcgataaagt gaagctgtct ccacagaagg aatcgccgaa
     2341 gttatattgc attcatacat taaaaatccg cgagcagaat tttgttgtgc agctgtaatg
     2401 taagcaacat ccgagcttgc ttcacttgta ttcattgcta aatcggtttg gtagcaagtc
     2461 aatgtcatcc ctccaaacaa gtaatcggtg gcacccataa gtacgccctt ataaacagca
     2521 attctggcgc cttcagctcc ataaaatgaa tcctgacgac caacaactcg acatttgttt
     2581 aaaattgccc tatcggcact tgccgtaaat gcaatagctg cagctctttc tacaaagttc
     2641 ttattctgta catccgtact cccgtaagtt gtaggacggg taccctttcc tccggttgac
     2701 cattcctgaa ctacatcttc cgattctttt cttgaaatat attgattaaa agaattttca
     2761 aaaattatat tgtcagctct gaaaccggaa gctgagacta ccactgttgc attccagtaa
     2821 gaaccgttag ttgtgccact tccggtattt gaataagttg tataaccatt ttccttattg
     2881 actctcagaa catctgcatt ccatttttgg tcggtgccca tgctgaaata attgtatccg
     2941 tgtccataat acgaagtaat tctcacagca ttttcatcaa tatccacacc tttgtttaaa
     3001 agagcaatac ttggtgttgt agaagcatta accaaagtaa cgtttggaat atcaatcacc
     3061 aacatttctt cgtaatttcc gggatctatc attattttta cagtatctgt attttcacgc
     3121 gtcatacaac gaacggcagc taatgcgtca ttgatactct gatattcctt atcggttcca
     3181 accgttatta ttggcttgta ggttacagat tttaccattt taatttcagt taaataggtg
     3241 gtcgttcctg taacattagc aatggtcatg taaccgggag tagattccgc ataaacaaat
     3301 tcgtgcgatt caatgcggtt ggtacttccg ctgttggtag aaatttcact tcctccatcc
     3361 acagtgaact tggcagaata atagtaataa actatcattt tttcaccggg tgaaacagga
     3421 actttagccg ttgcattacc tgtgagtacc aaatgtccct tcgatttttc attgtaaacc
     3481 gaaccggtaa acaacattcc tttataagcc gttgtaacac cacttgtaat ggtggcatca
     3541 tcaaaagacc aatccgtaac gggaataaac gataaatctt ttgaagtttc ggtcccattt
     3601 acttttaagt ttgacgtaag tcccaattgt acaggatatt cgtccaaacc actgcacgaa
     3661 atggaataga ctccatcacg caactgaata ttattcaaat cggtgaaaga ataaacataa
     3721 cccgattcat tcaggttgga gaaagtaaca gaaagtttgg aaagttgcgt agcgtctaaa
     3781 ccgttaatat ttaacaaaac agtataaacc ggcttagcta caaatacaat atcggtatta
     3841 atatctgaag agatatttac attattgttg gagatataat aatcatttac acctgtagca
     3901 gaaatcgtat aatcgcaatt tgcttccaac tgaacagaat aagtcccggc agaagcattc
     3961 actacaggtt tggggacata aattctggta tcagaaggag taaaaattaa tcctaatttc
     4021 gccagttttt cggaaggcaa tccagtaata ttcccactta atgtgtaaag tgtaactttt
     4081 aagataacag gattataagt tgtatttcca ctcaccgaca aagggtcgga attggatata
     4141 atatagccgt tggcattact gagacttaaa gaataagtgt atcctgcagg cagatttatg
     4201 gaatagttgt tagaagcatc aggagctact gaccaaacct ttccggcagc attggtaaaa
     4261 ttaatcgtgt atcctgaagg tatatccgaa gcgttggtca aatcaaacgt accactgacg
     4321 gaaacatacg tggcaggttt tctcaaaatt ctgtaataaa atggtttatc aacggaatca
     4381 taaattttat atacccccgc tttctttgct acaaatttat atatttccgg tgatgaagtt
     4441 aaagaaacga tgtctttctg agaattatcc gacgtatatt caaaagtcat tttcccggtt
     4501 ccattttgag atttggcata aatggtaacc tcatcgtctt cgtttaaatt taagcttaaa
     4561 tatcttccgg tagcagcgct ggcattaaca taaagatatc cggttaaagt ttctgaagtt
     4621 gaagctaccg ttaccggaac accatttgaa tcgtaacgcg taagattggt attggatgtt
     4681 ctcaagcggt cgcttgtaga agaagtactg gtccagctta aaataccgac ggtaaatgag
     4741 ctgggtaaat ttttccctgt ggttccgggt gttacgcccg gataccaact attaattgtt
     4801 gcttcatcca acatgttgtt gtaaatagta ggatcgaatt gtgccgcacc aaaatcccaa
     4861 acatctattt tggtttgagc ctttataatt gaaaaaccta caaagaagaa taatagcaga
     4921 aagtaaaata tttttttcat tggtattttt ttaaaattta ttgataaggt atgtctcttt
     4981 atttattcag caatacactc accattataa aaggggcaac cgctttcggg tcattgtccc
     5041 tgatgggttc ggaaatgtaa tattcataac tgccgtcgcg ataatttttt tcgccaccca
     5101 aaccggctac agcacaggca tttttcaatg aaatagttcc atcagaattt tcgacaataa
     5161 aagtttttac aaattgcttg taggcttttt tacctttctt gagaaattct ttgggcaaat
     5221 aacctttttg cgctcctttt acccaagtat aaataaacat ggcagaaccg ctcgattcga
     5281 cataatttcc cttttcattg attttatccg taacctgaaa ccacattcct gttttcggat
     5341 cacgatattt gtcgagcgag tagcacaaat cagtcaaaac tttaattatt tcgggtcgtt
     5401 ttggatgatt ttcaggtaag aaatccagca catccactat tgccattgca taccagccca
     5461 tgcttctgct ccagaaattg ggtgaagttc ctttcacagg gtcagcccat ttttgttggc
     5521 gactctcatc ccaaccgtga taatacagcc cggtggcagg atcataacaa tgtttagcaa
     5581 tgagcagaat ttgaaaagct acttcgtcaa ataatgccgg ttcattaaac cggtaagcat
     5641 attcagccaa aaaaggtgaa gccatataaa taccatccag ccacatttga tgcggataaa
     5701 tctttttgtg ccaaaaaccg ccttcgctgg tgcgaggctg cgtgtttatc tggctacgaa
     5761 gtaattcaat agcttttttg tatttttcat ctttagtttt ttcatataaa gtgaaaagga
     5821 atttgccaga gttaattcga tccaaattgt attcttcaag tttgtaccct tttatttgtc
     5881 cattttcctc aataatggta tcggcataac ttttggcata ctgaaaatat ttttcatcgc
     5941 cggtcttttc ccagactttt agaaaagcct gcaattcaag cccgttgcaa taatcccatt
     6001 tcagtttttt tgaaaaatca agcatccagc tttcaggatt acgtttcatt tccgaatcag
     6061 ccattcgaac gtaatatttt gattcttttg gagttaaact gaagcacgtt aatgaaaata
     6121 ttaccagcaa ataaagtttt ctcattgata atcggtcttt taaattaaaa ataaacttat
     6181 tttaaaaatt cattttttaa aaattttctc aaatcctttg attttttgag ttgcaacttc
     6241 ttttacaaac atttcggcta ccgttcgagc tccagcttca ttgaaatgag tattgtcatc
     6301 caatccattg ggatacaggc gactaatacc cggcgctatt ttatggaaat atttttttga
     6361 accttctaca ccttcgtgtt gcaaccattc agaagtgagt ttttctaaat cgatcattgg
     6421 aacccgataa tctcgagcaa cactgcgtat aatttcgggg taactgccgt gtgttggtac
     6481 aaggcgacct gtgttgtcga aatttcttcg ggctaccgga gtacaaagaa caggagtcgc
     6541 acctttcaat cttacttcgg caacaaatgc cacaaaattc agcctgaatt gcgccggatt
     6601 ggtataacga cccgtttttt ctacagactc gtcattgtgt ccaaattgaa taatcacaaa
     6661 atcacctttt tcggtattgt tcaataactc tgtccacaat ccttcttcta taaacgatct
     6721 ggtacttctg ccatttctgg cataattttt caccaaagcc ttatcgttga gcaattcggg
     6781 caataattgt ccccatcccc gctcaagaaa aggttcttca taagattttc ccgtcaaact
     6841 gtcaaaaact gttttataca gcaccttatc ggccattgta gaatcacctg ccatcaaaac
     6901 cttgacaggt ttaggtgctg aagcaagaaa aaacaacatc aaaacaccaa gaatcgtaat
     6961 tgctgagaac ctgaacttca tttgatttta acatttgaaa taattatatt ttcgttttcc
     7021 tctcctttca gttcaattgc attttgaagt ttttcgggat aggtgaaatt ttcggcttta
     7081 aaattttcta tccgatttaa acgcagagca ggaccgattg tcggttcaac ataaatgttt
     7141 ttgaatacta tatctttgga attattcaat tctgctccat aagtagctgt gataattgca
     7201 ttttcaacct gaatgttgct gattttcatt tcaggcaacc cgttgaaaaa cattgctctg
     7261 tgtgcgttgc gcgaaactat gttttttacg aaaatatttc tgaaaacggg tgtctcttct
     7321 gtaacaggca tcaatacagc atctttctga ttatcgtttc cttcttccat agcttctgta
     7381 gcggacttgc caccataata caaatcaaat aaaaatgagt cggttaaaat atcgaacatg
     7441 taaattccgt caacaaaaat attctcaaca acgccaccac gtccacgttt acttttgaag
     7501 cgaagtccca catccgttcc taaaaactga cagcaactga cagaaatatt tcttactcca
     7561 cccgacattt cacttccaac cacaaaacca ccatgagctt tgaaaacctt acagttgtga
     7621 acaattaaat tttcggtagg aacagctcta cgacggcctt cttcgtcttt gcccgattta
     7681 atgcaaatgg catcatctcc tacatcaaaa taactattga ctatgatgac atttttacag
     7741 gattccacat caattccatc cccattttga gcataatcgg gatttcggac gataatgtta
     7801 tcaacaatca cattttcaca cataaaagga tgaatgttcc agcttggaga attttcaaat
     7861 aaaacaccct gcaagaaaac atttttgcat tgaataaaac tcaccataac cggacgaaga
     7921 aaatccctta tttccatcca ttcttcatca gtcagttttg cacgcggaac attcatgtcg
     7981 cttatcgagt atcctttgta tgatttttcc gatggaaacc aataatttcc ctcttttaca
     8041 actccgcccg aattgaccac ttttttccat tccgaagctg taacttttga ttttttcaaa
     8101 ggcctccatg cttcccccga tccattaatc gaaccttctc cagtaatggc tacattttca
     8161 atgttccttc cggaaatggg tgattggcag cgtttggttg ccaaaccttc aaagtaggta
     8221 tctaccaaag gatataaatc aaaatctttt gaaaaaagaa ttaatgctcc tttttccaga
     8281 tgaagattaa tatttgattt taattcaata ggtccagtaa accatacacc tgcaggtaca
     8341 attaatttac ctccaccttt tcgggcaaga gcatcaattg cctgtttaaa agcttc
//
LOCUS       DAOSCH010000088         8218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5664_length_8218_cov_6.912655, whole genome shotgun sequence.
ACCESSION   DAOSCH010000088 DAOSCH010000000
VERSION     DAOSCH010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8218
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5664_length_8218_cov_6.912655"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..2019
                     /locus_tag="PLZ54_07015"
     CDS             262..2019
                     /locus_tag="PLZ54_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HOJ66712.1"
                     /translation="MKKFLIPLFAVLVILLAVGFKASKDKQQKKIKITTAYLLLPVEE
                     NADEVKTTIKYPDKKLPDIFWIRFAQRKVDYWVKLEVGKYKGKNLELIFDGPSASKFG
                     IKRIRPADQFKINYAEPYRPDFHFSPEYGWMNDPNGLTYFEGEYHLFYQYNPFGTRWG
                     NMSWGHAVSTDLTSWTYLPVALWPDTLGAIFSGSVVIDKNNTAGFGKNAMIAIYTSAG
                     KVQSQSLAYSTDKGRTFNKFEKNPIIPNPGITDFRDPKVSWYQPENKWIMALATKQTV
                     TFYSSLDLKNWNRESEFGEGIGSHAGVWECPDLFPLNYDGKTKWVLLVSINPGGPNGG
                     SATQYFVGDFDGKKFQADPLPYPLWIDYGRDNYAGVTFSNIPEIDGRRIFLGWMNNWD
                     YANQTPTKIFRSSNTVPRELQLVNNGNHCVLSSFPVKEVDLLRGETVDFPDQIVNNEK
                     SLKEILKQNDGSFELNMVLKPKKGNVFGFSLQNSKGETLDFIFDLSTQFFKIQRNKSG
                     LIDFSDRFPLGMNAPLASKRSYEIRLLIDKCSAELFINHGEIVMTSLFYPREAMNSLK
                     FFSQEGTLKVEDIKVSNLK"
     gene            2032..3393
                     /locus_tag="PLZ54_07020"
     CDS             2032..3393
                     /locus_tag="PLZ54_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HOJ66713.1"
                     /translation="MLLNKYKLMKNRYLYWTTLVAINGGLLFGLNMAGISGAVDMIKG
                     EFSLTDSGLGTVAGLLTLGCLLGALFTGNFAEKYGRKKVMISTAIIYIVSALGCSFAE
                     SSGMLIVFRVLSGLAVGASSVVCPMYISEIAPAYNRGRLVSFNQFAITIGILLAYVFD
                     YILAGLGENSWRYMLLIPAIFGVLYLIFLLLKFPESPRWLLSKNKKEEAVKIMKAIGG
                     DELIATELPEMEKMINADIRKEKVKFIELFQGKIGKIVLIGTLLAAFQQITGINAVIM
                     FAPDIFQAAGSAKMDSLMQSMIVGLVNFLMTVVALWLVDKSGRKSLLFWGAIGMILSL
                     AYLTFEFSRPLQNSTGVLIALLVYISFFAASFAPVMWVVISEIYPARIKGLAMSFSTA
                     VSWLCTFLTVYFAPVIQGKLGFEYLFGIFCAFSLLAAVFVKFWIPETKGKSLEIIEKE
                     LGL"
     gene            3421..4311
                     /locus_tag="PLZ54_07025"
     CDS             3421..4311
                     /locus_tag="PLZ54_07025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HOJ66714.1"
                     /translation="MKDIIVGIGEILWDVLPTGKVLGGAPANFAYHISQLGFDGFAVS
                     AIGNDELGEEIIENLHRKNMNFIIEKVSFPTGTVQVTLNDAGIPCYEICENVAWDNIP
                     FTEEMKKLAQETKAVCFGSLAQRHPVSRQTIQQFLDIVPQEALKIFDVNLRQHFYSKE
                     LIDYSLKKCNILKINDEEILLLSQLFDWNEKSDIDICKRLLRDYSLDMIVLTKGTNGS
                     CVVTPHEISSKPTPIVEVADTVGAGDAFTAGFVAGILKGKSVPDAHELAVEISAFVCT
                     QQGAMPTLPQELINKISSPV"
     gene            4387..7143
                     /locus_tag="PLZ54_07030"
     CDS             4387..7143
                     /locus_tag="PLZ54_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008675339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOJ66715.1"
                     /translation="MKKAFLFNLIVLLFFLPSKALPIKKEFLIGVSQCSDDAWRRTMN
                     EEILREASFYPNLKVKIKTAFDSNQKQIRDIESFIEDKVDILIVAPNEAIPLTPVIEN
                     AMKCSIPVILVDRKINSDNYTAFVGADNYQIGKEVGIYVANLLKRKGNIVEITGLKGS
                     TPAVERHKGFRSVIDNYPQIKIVYENDGGWLRDEAREKMKNAIRLNIPIDLVFAHNDE
                     MATGVYEAYQVYNEKEKPKIIGIDALPGTNGGIQKVLDGILDATFIYPTGGEKAVQIA
                     VRILRHEPYDKENILYTAVVDKTNARVIKLQTDQIIQHQNRIAQLNQVLNKNLSQYST
                     QRIFLVLSLTVLFVILLLLALLFKSYKHKNKVNRELEIRNAAINRQKEELAEQRDQLL
                     MLSKNLEEATQAKLVFFTNISHEFKTPLTLILGPLDRLQEKENLSEEGKQLILLMRKN
                     VHLLLKLIDQIIDFRKTETGKMQMTFTFGDLKLFISDICQSFRFLAQKKHIHFNFFSD
                     GDDFVLWFDSDKMEKICYNLISNAFKFTPENGKIEVKLSKYALNDEFFAKIIVSDSGI
                     GMPNSQLDKIFDRFFKIGNNTQNSGIGLHLTKMLVELHNGIIEVQSVEGKGSTFTVTI
                     PFKQKDIAMSEQYPDINFERNYQEKINYMDVSDEINDEYNAEKPLILLVDDNADVRNF
                     IKTLLQNEYTLIEAKDGQSGLLKAMKYVPDLIVSDVMMDKMNGFELCKAVKENISTSH
                     IPVILLTAYAMDEQRSLGFESGADAYIPKPFNENLLKIRIRKTIENRKKLKEYFQNHL
                     AFGDKKELLNEVDNNFLERFRRIIEENLMDTELNIDDIGKSMGLSRVQLYRKVKSLTN
                     YSPNEFIRIIRLKTAEQMMITGEKSISEIAYDTGFTSPSYFTKCFKEYFNENPTDFLK
                     RIKN"
     gene            complement(7507..7845)
                     /locus_tag="PLZ54_07035"
     CDS             complement(7507..7845)
                     /locus_tag="PLZ54_07035"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HOJ66716.1"
                     /translation="MIYEKRGTIPNRTDISQRPPEVEQRERFGDLGIDLIIGKNHKGA
                     ILTINDRATGLGKLRKLESKDAEQLAMATIDCLMEWEPLLKTITPDNGKEFAAHQMDS
                     PTVKYRFLLC"
BASE COUNT         2651 a         1268 c         1595 g         2704 t
ORIGIN      
        1 tttatcgcaa tatccaataa aaaagagact gtctctactt ttgagacagc ctcttgctat
       61 ataatataaa atatagcgta ttataaattt ggattcaatg aataaatttt ttgttcagaa
      121 gtaattgatt tttactgttt ctcccgttgt aaattcattt aatgggagaa acagttattt
      181 gttgcaattc attgaaaatt aattccaaaa aaagtattct tactaattgt tagaaattta
      241 aggtaacccc agaaaacttt tatgaaaaaa tttcttattc ctctatttgc tgttttggtt
      301 attcttttgg cggtaggatt caaggcttcg aaagataagc agcaaaagaa aattaaaatt
      361 actactgcct atttattgtt acccgttgaa gaaaacgctg acgaagttaa aaccacaata
      421 aaatatcctg ataaaaaatt gccggatatt ttttggattc gttttgctca gcgtaaggtg
      481 gattactggg taaaattaga agttgggaaa tataagggca aaaacttgga attaattttt
      541 gatggtcctt ctgcatccaa gttcggaata aagaggataa ggccagccga tcaattcaag
      601 attaattacg ctgaacctta tcgtcctgat ttccattttt ctcctgaata tggttggatg
      661 aacgatccca acggtttgac ttattttgaa ggcgaatacc atctttttta tcagtataat
      721 ccctttggaa caagatgggg aaatatgagc tggggtcatg ccgtaagcac cgatctaact
      781 tcatggactt acttgcctgt ggctttatgg cccgatacat tgggggcaat tttttcggga
      841 agcgtcgtta ttgataaaaa taatactgcc ggttttggga aaaatgccat gattgctatt
      901 tataccagtg ccggaaaagt tcaatcgcaa tcgcttgctt acagtacaga taaaggaaga
      961 acctttaata agtttgagaa aaatcccatt attccaaatc ccggtataac tgattttcgc
     1021 gatccgaaag tgagttggta tcagcctgaa aataaatgga taatggcatt agcaacaaaa
     1081 caaacggtaa ctttttattc ttcacttgac cttaaaaact ggaatcggga aagtgaattc
     1141 ggtgaaggaa ttggctcaca tgcaggagtg tgggaatgtc ctgatttatt tccattaaac
     1201 tatgatggta aaacaaagtg ggtcttattg gtaagcatta atcccggagg acctaatggg
     1261 ggatctgcta ctcaatattt cgttggagat tttgatggca aaaaatttca ggctgatcct
     1321 ttgccttatc ctctttggat agattatggg cgagacaact atgcaggcgt cacttttagc
     1381 aatattcccg aaatagatgg tcgtcgaatt ttcctcggtt ggatgaacaa ctgggattat
     1441 gcgaatcaaa caccaacaaa aatttttaga agtagtaaca ctgttccccg agaattgcag
     1501 cttgtaaata atggtaatca ttgcgtgtta agtagttttc cagtaaaaga ggtcgatttg
     1561 ctcagaggcg aaacagttga tttcccagat caaattgtaa ataatgagaa atcgctgaaa
     1621 gaaatattga agcagaatga tggcagtttt gaacttaata tggttttaaa acccaaaaaa
     1681 ggaaatgttt ttggattttc gctacagaat tcaaaaggtg aaacattgga ttttattttt
     1741 gatttgtcca ctcaattttt taaaattcaa aggaataaaa gcgggcttat tgatttcagc
     1801 gatcgttttc cattgggaat gaatgctcca ttagcgtcta aaagaagtta tgaaattcgt
     1861 cttttaattg ataaatgttc tgcagaattg tttatcaatc acggtgaaat cgtgatgacg
     1921 tcgctttttt atccaagaga agctatgaat tctttaaaat tcttttcgca agaaggcacg
     1981 ttgaaagttg aagatataaa agtcagcaat ttaaaataaa aaatcattca aatattatta
     2041 aacaaataca aacttatgaa aaacagatat ttatattgga caactttagt tgctatcaat
     2101 ggaggattat tattcggctt aaatatggcc ggaatttctg gagcagtaga tatgattaag
     2161 ggtgaatttt cactcactga cagtggactt ggaacagttg ccggattgtt aacacttgga
     2221 tgtttgttgg gagctttgtt taccggaaat ttcgcagaaa aatatggtcg aaaaaaagtg
     2281 atgattagta cagctattat ttatattgtt tcagccttgg ggtgttcctt tgccgaatct
     2341 tcaggcatgt tgattgtttt tcgtgttttg tcaggattgg ctgtaggtgc aagttctgta
     2401 gtttgtccca tgtatatatc ggaaattgct ccggcctaca atagaggtcg actggtttct
     2461 ttcaatcagt ttgctataac cataggtatc ttattggctt atgtatttga ctatatttta
     2521 gcaggtttag gtgaaaacag ctggcgctat atgctcttga ttcctgcaat ttttggtgtg
     2581 ttgtatctaa tttttcttct tttaaagttt cctgaaagtc ctcgttggtt attgtcaaaa
     2641 aataagaaag aagaagctgt gaaaataatg aaagcaattg gaggagatga attgattgct
     2701 actgaacttc ccgaaatgga aaaaatgatt aatgccgaca ttcgtaaaga aaaagtcaag
     2761 tttattgaat tgtttcaggg aaaaattgga aaaattgttt taattggaac tttattggct
     2821 gcttttcaac aaattacagg aataaatgca gttattatgt ttgcacctga tattttccag
     2881 gcggcaggtt cagccaaaat ggattcattg atgcaatcga tgattgtggg gttggtcaat
     2941 tttttaatga cagttgttgc tctttggtta gtagataaaa gtggtcggaa atctctttta
     3001 ttctggggcg caatcggtat gatcctttct cttgcatatt tgacatttga attttcacgt
     3061 cctttacaaa atagcacggg tgtattgata gctttattgg tttatatttc tttttttgct
     3121 gcttcttttg cgccagttat gtgggttgtt atttctgaaa tttatccggc aagaattaaa
     3181 ggtttagcca tgtcattttc tactgcagta agttggttat gtacttttct aaccgtatat
     3241 tttgccccag ttattcaagg aaaacttgga tttgaatatc tttttggaat attttgtgcg
     3301 tttagcttat tggcagcagt ttttgtgaag ttttggatac cggaaaccaa aggaaaatca
     3361 ctggaaatta ttgaaaagga attgggtcta taaaattaat ttggaaatta tcaaaaaaac
     3421 atgaaagata ttattgttgg aattggagaa atactttggg acgttttacc aacaggaaaa
     3481 gttttaggag gagcgcctgc aaattttgct tatcacattt ctcaattggg attcgatggc
     3541 tttgccgtga gtgctattgg aaatgatgaa ttgggtgaag aaatcattga gaatcttcat
     3601 agaaaaaata tgaatttcat tatcgaaaaa gtttcttttc caaccggtac cgtgcaagtt
     3661 acactgaacg atgccggcat tccatgttat gaaatttgtg aaaatgtggc ttgggataat
     3721 attcctttta cggaggaaat gaaaaaattg gcacaggaaa caaaagctgt atgctttggt
     3781 tctctggctc aacgtcatcc tgtttcaaga caaaccattc agcagttttt agatatcgta
     3841 ccccaagagg cattgaaaat ttttgatgtt aatttgcgcc aacattttta ttctaaagag
     3901 ttgatagact attcattgaa aaaatgcaac atactgaaaa tcaatgatga agaaattttg
     3961 cttttgtctc aactattcga ttggaacgaa aagtcggata tagatatttg taaaaggctt
     4021 ttgagagatt attcactcga tatgatcgtg ctcacaaaag gaacaaatgg cagttgtgtt
     4081 gttacacccc acgagatttc atccaaacca actccaatag ttgaggttgc cgatacggta
     4141 ggtgccggtg atgctttcac tgccggtttt gtggcaggaa ttctcaaggg aaagagcgtt
     4201 cccgatgctc atgaattggc tgtggaaata tcggcgtttg tttgtaccca acaaggtgcc
     4261 atgcccactc ttcctcaaga attgattaac aaaatttctt ccccagtttg atttttattt
     4321 ggtaaaaaat catatttttg tgttgtaaat cattttgata cctataatga aagttttggc
     4381 tgtttcatga aaaaagcttt tctttttaat ttaatcgttt tattattctt tttaccatca
     4441 aaggcattac cgataaaaaa agaatttttg attggtgtgt ctcagtgcag cgatgatgca
     4501 tggcgacgaa cgatgaatga agaaatattg agggaagctt ctttttatcc caatctgaaa
     4561 gtcaaaatta aaacggcttt tgacagcaat caaaaacaga tacgcgatat cgaaagtttt
     4621 attgaagata aagttgatat tttaattgtt gctcccaatg aggcaattcc tttaacacca
     4681 gtaattgaaa atgccatgaa atgtagtatt ccggtaatat tggttgaccg gaaaatcaat
     4741 tccgacaatt ataccgcatt tgttggtgcc gataattatc aaattgggaa agaagtcgga
     4801 atatatgttg ccaatttact gaaaagaaaa gggaatattg ttgaaataac agggctgaaa
     4861 ggctctaccc cagctgttga gcgccataaa ggtttcagaa gcgtgattga taactatcct
     4921 caaataaaaa ttgtatatga aaatgatgga ggttggttaa gagatgaagc aagagaaaaa
     4981 atgaaaaatg ctatccgttt aaacattccc atagatttgg tttttgctca caatgatgaa
     5041 atggcaacag gagtttatga agcttatcag gtttacaacg aaaaagaaaa accaaaaatt
     5101 atcggaattg atgcacttcc gggtaccaac ggtggtattc agaaagtttt ggatggcata
     5161 ctggacgcaa cttttattta tcctaccgga ggtgagaagg cagtccagat tgctgtaaga
     5221 attctccgtc atgaacctta tgacaaggaa aacattctct acacggctgt ggttgataaa
     5281 acaaatgcaa gagtaataaa acttcaaaca gatcaaatta ttcagcatca gaatcgtatt
     5341 gcgcaactta atcaggtgct aaataaaaat ttgtcacaat acagtacgca acgaattttt
     5401 cttgttctgt cgctcacggt gctttttgtt attttattgt tgctggcatt acttttcaag
     5461 tcatataagc acaaaaataa agtgaataga gaattggaaa tcagaaatgc tgctataaac
     5521 agacagaaag aggagctggc cgaacaacga gaccaattac tgatgctttc taaaaatctg
     5581 gaagaggcca cacaggcaaa actggttttc tttaccaaca tctcacacga attcaaaacg
     5641 cctttaacgc ttattctcgg ccctttggat cgattacagg aaaaagaaaa tttatctgag
     5701 gaaggtaaac aattgattct tttaatgcgc aaaaatgtgc acttgcttct taaattaatt
     5761 gatcaaatta ttgatttccg aaaaaccgaa accggaaaaa tgcaaatgac ttttactttt
     5821 ggcgatctaa aactttttat ttcagatata tgtcagtctt tccgtttttt agcccaaaag
     5881 aagcacattc attttaattt cttttctgac ggtgatgatt ttgttttgtg gttcgatagc
     5941 gataaaatgg agaaaatttg ttataacttg atttctaatg ctttcaaatt tactccggaa
     6001 aatggtaaaa ttgaagtaaa attatcaaaa tatgctctca acgatgaatt ttttgcaaaa
     6061 ataattgtgt ccgattcggg cataggaatg ccaaactcgc agttggacaa aatttttgat
     6121 agatttttca aaatcgggaa caacactcag aattccggaa ttggtttgca tttgacaaaa
     6181 atgttggtcg aattgcacaa tggaatcata gaagtacaaa gtgtcgaagg taaaggttct
     6241 acttttactg taaccattcc tttcaaacag aaagatatag ctatgtccga acagtatccg
     6301 gatattaact ttgaacgaaa ttatcaggag aaaattaatt acatggatgt gtctgatgaa
     6361 attaatgatg aatacaatgc tgaaaaacct ttgattttat tggtagatga taatgctgac
     6421 gttcgtaact tcatcaaaac attgttgcaa aatgaatata ctctcattga agccaaagat
     6481 ggacaaagcg gattgctcaa agcaatgaaa tacgtacccg atttgattgt tagcgatgtg
     6541 atgatggata aaatgaatgg atttgaattg tgcaaggcag taaaagaaaa tatttctacc
     6601 agccatattc ccgttatttt attgacagct tatgctatgg acgaacaacg ctctttggga
     6661 tttgaaagtg gcgccgatgc ttacataccg aagcctttta atgaaaatct tttgaaaatc
     6721 agaattagaa aaaccattga aaacagaaaa aaattaaagg aatattttca gaaccacttg
     6781 gcttttgggg ataaaaaaga attgttgaat gaagttgata ataacttttt ggagagattc
     6841 agaagaatta ttgaggagaa tttgatggat accgaattaa atattgatga tattggcaaa
     6901 tctatgggtt tatcacgcgt ccagctttac cgaaaagtta aatcattgac aaactattct
     6961 cccaatgaat ttattagaat cataaggtta aagactgccg agcaaatgat gattacaggt
     7021 gaaaaaagta tttcggaaat tgcttacgat accggtttta cttcaccttc ctatttcacc
     7081 aaatgtttta aggagtattt caatgaaaat cctaccgatt tccttaaacg tattaaaaat
     7141 tgattttaaa ttatttcaat ttttgtttgc acacctgttg tatcattgcc caaattgtgt
     7201 acttttgtta tactataaaa atgtttcatc ttgtaacttt ttaaaatgga agagggcgca
     7261 gtaaagcatt tatttcaaca gggaaggggg aagaagagtt tttttctttg tcttcaaaaa
     7321 aattatttcc ctttcctttc atttcccctg aaaaagtcgc attaatcact tgaatttacg
     7381 tttgcgtttc gacaaataat ataatcctga gagatacatt caaaatcgac ttttttcggt
     7441 atatattgcc taacgaggcg attataattt tcattcgatc ctcttttttg ccagcttgca
     7501 tatggtttag caaagaagaa atcgatattt aactgttggg ctatccatct gatgagcggc
     7561 aaactctttt ccgttgtctg gagtgattgt tttaaggagt ggttcccatt ccattaaaca
     7621 atcaatggta gccattgcaa gttgttcggc atctttgctt tctaatttac gaagtttccc
     7681 taatccggta gctctgtcat tgatggttaa gatagcgcct ttgtgatttt tgcctataat
     7741 caaatcaatt cccaaatcac caaaacgttc tcgttgttca acttcaggcg gtctttgaga
     7801 gatatctgta cgattgggga ttgttccccg tttctcgtat atcatacttc gtttttcctg
     7861 ttttccacat ttttttgcaa cacccttata agtatttgaa tatcaataat aatattttaa
     7921 ttttgggtgt tgcaaaatta ttattatgtt tatccgaaaa gtaaaaaatc gatctggaag
     7981 tcaatctgta caagtgatac agaagtttcg aggaaagtat aaagtagtga aaaccattgg
     8041 ttgtgccacc acgcagcacg aaattgaaag gttggaacaa ttggtcagag aagaaatgaa
     8101 tcgttacaaa ataattgtag aaacatttat attctatatt ttcagatatt taataacggg
     8161 gctttgcccc aaaccccact tacttttttg tcttgaaaca aaaaagtaag caaaaaaa
//
LOCUS       DAOSCH010000089         8180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5702_length_8180_cov_5.466462, whole genome shotgun sequence.
ACCESSION   DAOSCH010000089 DAOSCH010000000
VERSION     DAOSCH010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8180
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5702_length_8180_cov_5.466462"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1552)
                     /locus_tag="PLZ54_07040"
     CDS             complement(80..1552)
                     /locus_tag="PLZ54_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HOJ66717.1"
                     /translation="MGDNLKQKTLSAFTWTTIDRFGQQLVQLIIGIILARLLSPGDYG
                     LMGMVMIFAALSYVLVESGFGQALIRKTEANETDYNSVFYFNIGIALVLYALLYFLSP
                     AIANFFKQPELVSVNRITFLAIIFNAFYLVPYSKSAKILDYKTIAKVNIISVSLSGLL
                     GLTLAFFHYGIWVLVIQHVSYHFFRMIFFYVFVRWQPKLIFSFRVIRSFWKFSIHLLN
                     TSILNIIFNYLFVIILGKFYKKSEVGYYMQGNKLSETFNFTFQSILLGSTFSLFSHIQ
                     HDDARFRRIFGELSNKISIITFPIILCLIAAGKPLIEIVWSAKFLPSVPYFRLLCLAS
                     LFLPLYTLNISALNARGQSRITFRIEMIKKILILIAIFITFSKGIIPMLWGYAVANIA
                     AYFLSIFYIKSELKHDLKQQILAFIQSLGWGLVLGLMVASLSLLIKPSYFLLPVQIFL
                     AAAVYLLIIKIFYKELYDKGMDFLKNRLLSLKQGLHQNKF"
     gene            complement(1574..2461)
                     /locus_tag="PLZ54_07045"
     CDS             complement(1574..2461)
                     /locus_tag="PLZ54_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOJ66718.1"
                     /translation="MAKFNPFTKKNWIFIRQYILITIGLFMYAAAWKGFLLPHQITGG
                     GVTGIGAIVYYATGVPIFITYFVINALLIAVALRIIGFQFSLRTIYGVAMITLFFAVL
                     PQADPGTFVAPNDNFMACLLGGLISGTGLGIVFLNNGSSGGTDIIAKIVNKYYNITLG
                     RILLYCDVLIICSSYFFEFGTIEKIVYGLTNMAISNLAIDMVINGVRQSVQFFIFSKE
                     YEQIANRINTEVHRGVTILDGTGWYSQQPVKVITVIARKNESSMIFRIVKEIDPDAFV
                     SQSSVIAVYGKGFDVIKSK"
     gene            complement(2502..5258)
                     /gene="leuS"
                     /locus_tag="PLZ54_07050"
     CDS             complement(2502..5258)
                     /gene="leuS"
                     /locus_tag="PLZ54_07050"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOJ66719.1"
                     /translation="MEYNFREIERKWQTYWKDNQIYKTVENPSKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYSRYKRLKGFNVLHPMGFDAFGLPAEQYAIQTGQHPAITTKN
                     NIARYREQLDKIGFSYDWDREIRTCDPDYYHWTQWAFIQMFHHYYDNNLQKAQPISRL
                     VEHFEKKGTENLHASGTRELSFTAADWKALSEKQQQEILLNYRIAYLADTKVNWCPAL
                     GTVLANDEVSEGVSIRGGYPVEQRIMRQWCLRVSAYAQRLLDGLETIDWTDSLKETQR
                     NWIGRSEGAEIRFSIKGREETFEVFTTRADTIFGVTFMVLAPESELVKICSTPQQAAE
                     VEKYLENTKKRTERERLADRRVTGVFSGSYAIHPVTGKEIPIWISDYVLAGYGTGAIM
                     AVPAHDSRDYAFAKHFNLPIIPLIEGCDVSEESFDAKEGIMINSGFLNGLTVKEAIEA
                     TKKYITEKGIGRVKVNYRLRDAIFSRQRYWGEPIPIYYKDGVPYTLSEEELPLELPEV
                     DKFLPTEKGEPPLARAKNWKTKEGYPLEVCTMPGFAGSSAYYLRFMDPKNDQALVSPE
                     ANQYWRNVDLYLGGTEHATGHLIYSRFWNKFLYDLGIVCEEEPFKKLINQGMIQGRSN
                     FVYRIKDTNTFVSYHLKDQYETTPIHVDVNMVSNDVLDLEKFKNWNPEYKNAEFILED
                     GKYICGWAIEKMSKSMFNVVNPDDIVENYGADTLRLYEMFLGPLEQSKPWDTNGIDGV
                     HRFLKKLWNLFYKNDELCVTEEKPTAEELKVLHKTIKKISYDIENFSYNTAVSAFMIC
                     VNELFTLKCNKKAILEPLLILLAPFAPHISEELYHALGNSDTVFNASFPQYEEEFLKE
                     NTVSYPVSFNGKVRFTLEMPTDVSKEEIEKTALSHPQTIKYMEGKTPKKIIIVPGKII
                     NIVF"
     gene            5463..6116
                     /locus_tag="PLZ54_07055"
     CDS             5463..6116
                     /locus_tag="PLZ54_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOJ66720.1"
                     /translation="MSDIKFNDELRVPEPTLRRLPWYLAYAKLMMEKGETYLSSTQIS
                     NNIAVDASIVAKDLSYVHISGKTRVGYETHELIIVLERFLGFTESHKAFMFGVGNLGG
                     ALMHDNGLEQFGLKIVAGFDVKYELAGTSINRIPIHHIEKFPELQRQTGVIIGILTVP
                     VDKAQEVSELMIEGGIRAIWNFTPFRIRVPDNVVVQNTSIYAHLAVMFNRLNAIVNK"
     gene            6148..6759
                     /locus_tag="PLZ54_07060"
     CDS             6148..6759
                     /locus_tag="PLZ54_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007842179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOJ66721.1"
                     /translation="MKIFAIGQNYPEHNKELKNDNPVEPVVFMKPDTALLKNNKPFYI
                     PDFSNELHYETELIIRFNRIGKNIQSKFAHRYFDEIGLGIDFTARDLQRKLKAEGKPW
                     EICKAFDNSAVVGDFLPASDFRDIQDIQFHLDINGITVQKGDSKDMIFSVSELIAYVS
                     RFFTLKIGDILFTGTPAGVGCVHPGDRLEGYIGNRRMFNFLVK"
     gene            6913..>8180
                     /locus_tag="PLZ54_07065"
     CDS             6913..>8180
                     /locus_tag="PLZ54_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66722.1"
                     /translation="MAQNTITTSHKLIWKGIEKWYADSSSVQVISFRDAHYPYENRLP
                     YFNYRIPAENYSYKAVLTNTVFEAVNTEEAAILSDKAVYSSEPVLETRYLVDRGSSFF
                     DVQMLPFVFQNGKFLKLISFDLSISKTALPQQAKNAFTHSFASSSVLAQGKFVKVRLA
                     DSGIYKLTYEDLVAMGINPSNVRVFGYGGGVLDQNFTQTKPDDLPEVPIWVEKGSDGI
                     FNAGDYILFYAQGVVRWEYDNGKSMFVHKNNSYSQYGYYFITSDAGIGKKIESKPIEL
                     PANATIYQVDEFTDYQLYEKDIISLTKSGKEFYGETFSTKLSYSFAFYFPNVVKNNTL
                     KFAIDVAASSMSNSSFSINLNGSQSKTLSVTKNSGDNYEMAKSAFAVYTFTPDDETLT
                     FNITYNKSTSTSTGYLNYMEVNARRKLIMQG"
BASE COUNT         2503 a         1547 c         1562 g         2568 t
ORIGIN      
        1 cgatagataa aaatttttta aggaacagag ctactgaata ctcatggaat aatattacta
       61 aaatggaatt tgatatatat caaaatttat tctgatgtag cccttgtttt aaactgagca
      121 gtcgattttt tagaaaatcc atacctttat catacagttc tttgtaaaaa attttgataa
      181 ttaacagata aacagctgct gcaagaaaga tttgaacggg cagcaaaaaa taggagggtt
      241 ttatcagcaa acttaaggaa gcaaccatca agcctaaaac aagtccccaa cccagactct
      301 gaataaatgc cagtatttgt tgtttcaaat catgtttcaa ttctgatttt atgtagaata
      361 tcgaaagaaa ataggctgca atatttgcaa ctgcgtagcc ccagagcatg ggaattattc
      421 ctttgctgaa cgtaataaaa atggcaatca agataagtat tttttttatc atttctattc
      481 tgaaagtgat tcgcgactga cctcttgcat tcaatgcact gatattgagt gtgtaaagtg
      541 gtaaaaacaa ggaagccaga caaagaagcc ggaaataagg gacagacgga agaaattttg
      601 ccgaccaaac aatttctatc aatggttttc cggcagcaat cagacacaaa attatgggaa
      661 aagtaataat ggagattttg ttcgacagct caccaaaaat tctcctgaaa cgggcatcat
      721 catgctgaat atgagagaaa agggaaaatg tactgcccaa taaaatggat tgaaaagtaa
      781 aattgaaagt ttcacttaat ttgtttcctt gcatgtagta tccaacttcc gattttttgt
      841 aaaatttccc caaaataata acaaacaggt aattgaaaat aatatttaaa atggaagtat
      901 tcagtaaatg aatgctaaat ttccagaaac tacgaataac ccggaatgaa aaaatcaatt
      961 ttggttgcca tcggacaaag acataaaaaa atatcattct gaaaaaatga taggaaacat
     1021 gttgaattac cagcacccag atgccataat gaaaaaaagc aagtgtaagg cccagcaaac
     1081 cgctgagcga aacagaaata atattgactt tggcaatggt tttataatca agaatttttg
     1141 ccgattttga atagggaacc agataaaaag cattgaaaat aatggccaga aaagtaattc
     1201 tattcaccga aacaagttcg ggttgcttaa aaaaattggc aatagccggc gaaagaaaat
     1261 acaaaagtgc atacaaaacc aaagcaatac cgatgttgaa ataaaataca ctgttgtagt
     1321 ccgtttcatt ggcttcggtt tttctgatca aggcctgtcc aaatccactt tcaacgagca
     1381 catacgaaag tgccgcaaaa atcatgacca tgcccattaa tccataatcg cccggagaaa
     1441 gaagcctagc cagtataatg ccaataatca actgaaccag ctgttgaccg aagcggtcaa
     1501 tggtcgtcca agtaaaagcg ctgagggttt tttgtttcag gttatcgccc atgtggtaat
     1561 ggtacaataa aaattatttg ctcttgatca catcgaagcc ttttccgtaa acggcaatca
     1621 cggatgattg ggatacaaag gcatcgggat caatttcttt tacaattctg aaaatcatgg
     1681 aagattcatt ttttcgggca atgacggtaa taacttttac cggctgttgc gaataccatc
     1741 ccgtcccatc cagaatggtt acgccccgat gaacttccgt attgattcgg ttggctattt
     1801 gttcgtattc ttttgaaaaa ataaaaaatt gaacagactg tcgtactcca ttgataacca
     1861 tatcgatggc caaatttgaa atagccatgt tggtcaagcc gtacactatt ttttcaatgg
     1921 ttccaaattc aaaaaaatag gaagagcaaa taatcagcac atcgcaataa agcagtatcc
     1981 tgcccaatgt aatgttgtag tatttgttga cgatttttgc gataatatcc gttccgccgg
     2041 aactgccgtt attgaggaaa acgataccca atcccgttcc cgaaatcaaa cctcccagca
     2101 aacatgccat aaaattatcg tttggagcaa caaaagttcc cgggtctgcc tgtggaagca
     2161 cggcaaaaaa cagggtaatc atggcaaccc cgtaaatggt gcgcaagctg aactgaaatc
     2221 cgataatacg cagtgctaca gctatgagca gggcattaat aacaaaataa gtgataaaaa
     2281 tcggaacccc cgttgcataa taaactattg ctcctatacc tgtaacacct ccaccggtaa
     2341 tttgatgcgg cagcaaaaaa cctttccatg ctgcagcata cataaataaa ccgatagtta
     2401 tcaggatgta ttgtcggata aatatccagt ttttttttgt gaaaggattg aattttgcca
     2461 ttatttattt gggattgaat gtttttcctg aatttttctt gtcagaaaac aatattaata
     2521 atttttcccg gaacaataat aattttcttc ggcgtttttc cttccatgta ttttatggtt
     2581 tgtggatgtg ataaagcagt tttttcaatt tcttctttgc tgacatcggt cggcatttcc
     2641 aacgtaaatc ttacttttcc gttaaaagaa acagggtaag aaaccgtatt ttctttcaaa
     2701 aattcctctt catattgtgg aaaagaagca ttgaaaacag tatcgctatt gcccaatgca
     2761 tggtaaagtt cttccgaaat gtgaggggca aaaggtgcca gcagtatcag caaaggttca
     2821 agtatggctt ttttattgca cttcagagta aacaattcgt tcacacaaat catgaacgcc
     2881 gaaacagctg tgttatagga aaaattttca atgtcgtagc tgattttttt aatggttttg
     2941 tgcaaaactt ttaattcttc tgcagttggt ttttcttctg ttacacaaag ttcatcgttt
     3001 ttgtaaaaga gattccacaa ctttttcaaa aagcgatgca cgccgtcaat tccattggta
     3061 tcccaaggtt ttgactgttc cagtggaccc aaaaacattt cgtacaaccg aagggtgtct
     3121 gcgccgtaat tttcgacaat atcatcaggg ttgacaacat tgaacatcga tttcgacatt
     3181 ttttctatag cccaaccaca aatgtacttt ccatcttcaa gaataaattc ggcatttttg
     3241 tattcggggt tccagttttt aaatttttcc agatcgagaa catcgttgga aaccatgttg
     3301 acatcaacat gaatcggagt tgtttcgtat tggtctttta aatgatagga aacaaaggta
     3361 ttcgtgtctt ttattcggta cacaaagttg cttcttccct gaatcatgcc ttgattaatg
     3421 agttttttaa atggttcttc ttcgcaaacg atacccaaat cgtacaaaaa tttgttccaa
     3481 aaacggctgt aaatcaaatg acccgttgca tgttcggttc caccgagata aagatccacg
     3541 tttctccagt attggttggc ttcagggctt acaagtgcct ggtcgttttt cggatccata
     3601 aatcgtaaat aataagctga agaacctgca aaaccgggca tagtacagac ttcaagcgga
     3661 tagccttctt tggttttcca gtttttagcg cgagccaatg gcggttcacc tttttcggta
     3721 ggcaaaaatt tgtccacttc gggcaattca agcggtagtt cttcttcgct aagtgtgtag
     3781 ggcactccgt ctttgtaata aatgggaatg ggttcgcccc agtaacgttg gcggctgaaa
     3841 atagcgtcgc gtagacgata attgactttt accctgccta ttcctttttc cgtaatatat
     3901 ttttttgtgg cttcaatggc ttcttttacg gtcagtccat tcagaaatcc cgagttgatc
     3961 atgatacctt ctttggcatc gaaactttct tcactgacat cacacccttc gataagcgga
     4021 atgatgggca aattgaaatg tttggcaaaa gcgtagtctc tgctgtcgtg cgccggaaca
     4081 gccataatag cccctgttcc gtaaccggca agcacataat cggaaatcca aatgggaatt
     4141 tctttcccgg taacaggatg aatggcataa gaaccggaaa aaactcccgt aacacgccga
     4201 tctgccaagc gttcacgttc tgtacgcttt ttggtgttct cgaggtattt ttcaacttca
     4261 gctgcctgtt ggggagtgga acatattttt accaattcgc tttcaggagc aagtaccata
     4321 aatgtaacgc caaatatggt atccgcacgt gtggtaaata cttcaaaagt ttcttctctg
     4381 ccttttatgg aaaaacggat ttcggcacct tcacttcttc ctatccaatt cctttgtgtt
     4441 tctttcaggg agtcggtcca gtcgatagtt tccaaaccat ccagcaaacg ttgggcataa
     4501 gccgaaacgc gcagacacca ttgcctcatg atacgttgct ctacaggata tccaccgcga
     4561 atggaaacgc cttcgctcac ttcgtcgttg gccagcaccg tacccaaagc cggacaccag
     4621 ttcacttttg tgtcggccaa ataggcaatg cgatagttta acaaaatttc ctgttgttgt
     4681 ttttcgctca acgctttcca gtcggcagcc gtaaacgaca gttcacgagt gcccgaagca
     4741 tgaagatttt cagtaccttt tttctcaaaa tgttcaacaa gacgactaat gggttgagct
     4801 ttctgcaaat tgttgtcata ataatgatga aacatttgaa tgaaggccca ttgtgtccag
     4861 tggtaataat cgggatcgca ggttctgatt tctctatccc aatcgtaact gaatccgatt
     4921 ttatcaagtt gttcgcgata gcgagctatg ttgtttttgg tcgtaatggc cggatgttgg
     4981 cctgtttgaa tggcatattg ttctgccggc agtccgaaag catcaaagcc cattggatgg
     5041 agtacattaa aacctttcag tcttttgtaa cggctgtaaa tatccgatgc aatgtaaccg
     5101 agcggatggc cgacatgcag ccctgctccc gaaggatagg gaaacatatc caatacataa
     5161 tatttgggtt tgcttgggtt ttcaacggtt ttataaatct gattgtcttt ccaatatgtt
     5221 tgccattttc gctctatttc tctgaaattg tactccatgt atcactgaaa tttaataaat
     5281 tcaaccttat cttttctctt ttacagtatt cgcaaattaa atttgcaaag ttaatcattt
     5341 tgccaaaata aaagaaagct tcttcttttt ccgtttcatc tttcgctcat ttttataaag
     5401 tataagtata aatatttcaa taatttgatt atttttgcag gtgattacta atctccagaa
     5461 agatgtccga tattaaattc aatgatgaat taagagttcc cgaaccaaca ttgagacgac
     5521 tgccatggta tttggcctat gcaaaactta tgatggaaaa gggtgaaacc tatttgtctt
     5581 ctactcaaat atcgaacaac attgctgtag atgcttccat tgttgccaag gatttatctt
     5641 atgttcatat ttcgggaaaa acaagagtcg gttatgaaac ccatgaattg attatcgttt
     5701 tggaacgctt tcttggcttt acggaatcgc acaaagcttt tatgtttgga gtcggaaatt
     5761 tgggaggtgc tttgatgcac gacaacgggc tggaacagtt tgggttaaaa atagtggcag
     5821 gttttgatgt aaaatacgaa ttggcaggta ctagtatcaa ccgcattccc attcatcata
     5881 ttgaaaaatt tccagaacta cagcgacaaa caggtgtaat cattggcatt ttgactgtcc
     5941 ctgtggacaa ggcacaggaa gtgtcggaat taatgattga aggaggaatt cgtgcaattt
     6001 ggaactttac tccttttaga ataagagtgc ccgacaatgt ggtggtgcaa aatacttcta
     6061 tttatgctca tttggcggta atgttcaatc gcttgaatgc cattgttaac aaataatttt
     6121 tttatcattc attagttaaa ggaaaaaatg aaaatttttg ctatcgggca aaactatccc
     6181 gaacacaata aagaattgaa aaatgataat cctgttgaac ctgttgtgtt tatgaaaccc
     6241 gacacagcgc tgttgaagaa caacaaacct ttttatattc ccgatttttc caatgaattg
     6301 cattacgaaa cggaattgat tatacgtttt aaccgcatag gtaaaaatat tcagtctaaa
     6361 tttgcccatc gttatttcga tgaaattggt ttgggaattg attttactgc ccgcgatttg
     6421 cagagaaaac tcaaagccga aggaaaacct tgggaaattt gcaaggcgtt tgacaattcg
     6481 gctgttgtag gcgattttct tcctgcttca gattttaggg atattcagga tattcaattt
     6541 catttggata tcaatggaat aactgtacaa aagggtgatt ccaaagatat gattttttcg
     6601 gtcagtgaac ttattgctta cgttagtcgt ttctttactt taaaaattgg tgatatttta
     6661 tttaccggaa cacctgccgg tgttggctgt gttcatcccg gcgatcgttt ggaaggctat
     6721 atcggcaacc gaagaatgtt caattttctt gttaaataat tgagcaacta atccctgaat
     6781 aataactttt tgttttgaac gtttttaaat tatctgtttt tttgtattaa attttatttc
     6841 tcccttgagg gcgcttttat gaaaattaaa tctttatttt tttctttttt cctttctgtc
     6901 agtggttggt gtatggctca aaatactatc acaacttctc ataaactgat ttggaaagga
     6961 attgaaaaat ggtacgctga ttcatcttcc gttcaggtca tttcgtttcg ggatgcgcat
     7021 tatccttacg aaaatcgttt gccttatttc aattacagaa ttccggccga aaattattcg
     7081 tacaaagcgg ttttgaccaa cactgttttt gaagctgtca acactgaaga ggcagcaatt
     7141 ttaagcgata aagctgttta ttcttccgaa ccggtgctgg agactcgtta tcttgtcgat
     7201 agaggaagca gcttttttga tgttcagatg ttgccttttg tttttcaaaa cggcaaattt
     7261 ctcaaattaa tctctttcga tttatcaatt tccaaaactg ctcttccaca acaggcaaaa
     7321 aatgctttca cacacagttt tgcttcttct tcggtgctgg cacaaggaaa atttgttaaa
     7381 gtcagactgg ctgattcagg aatttacaaa cttacttatg aagatttggt tgcgatgggt
     7441 attaatccat ccaatgtcag ggtctttggt tatggcggtg gggttttgga tcaaaacttt
     7501 actcaaacca aacctgacga tttgcccgaa gttccaattt gggtggaaaa aggaagtgat
     7561 ggaattttca atgccggcga ctatatattg ttttatgcgc aaggcgttgt aagatgggaa
     7621 tatgacaacg gcaaatcaat gtttgtccat aaaaataatt cttattcgca atatggttat
     7681 tattttatta catccgatgc cggcattggt aaaaaaatcg aatcgaaacc cattgaactc
     7741 cctgccaatg caacgattta tcaggttgat gaatttacag attatcagct ctacgagaag
     7801 gatatcatca gtcttaccaa atcgggaaaa gaattttatg gagaaacatt tagcaccaag
     7861 ctttcttata gttttgcttt ctattttccc aatgttgtaa aaaataatac attaaaattt
     7921 gctatcgatg ttgcagcttc ctcaatgtcc aactcttcct tttctataaa tcttaatgga
     7981 tcacaaagca aaacgctttc ggtaacaaaa aattcgggtg ataattatga aatggcgaaa
     8041 tcagcttttg cagtttatac ttttacaccc gacgatgaaa cgttaacttt taatattact
     8101 tacaataaat caacttcaac ttccaccggc tatttgaatt atatggaggt aaatgcccga
     8161 cggaaattaa ttatgcaggg
//
LOCUS       DAOSCH010000090         8115 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5761_length_8115_cov_6.418486, whole genome shotgun sequence.
ACCESSION   DAOSCH010000090 DAOSCH010000000
VERSION     DAOSCH010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5761_length_8115_cov_6.418486"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1076
                     /locus_tag="PLZ54_07070"
     CDS             78..1076
                     /locus_tag="PLZ54_07070"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOJ66723.1"
                     /translation="MKTKVILFDAKSYDEIFFKEKNQQHGFDIKFQKSHLSKDNAILT
                     KGADVVCIFVNAEVDAEVIDILVENGVKLIALRSAGYNNVDLKAAKGKIKVVRVPAYS
                     PHSIAEHTIALMLTLNRKIHKAYTRTREGNFALEGLMGFEMNGKTAGIIGTGKIGKIV
                     IQILKSMGMNVLAYDLYPDTQFAETNGIRYTQLDELYGLSDVISLHCPLTKETEYLIN
                     EKSIHKMKEGVMIINTGRGKLIKTKALIDALKSKKIGYAGLDVYEEEGDYFYEDHSDI
                     VIDDDILARLLTFNNVIVTSHQAFFTKEALSEIARITLQNIQDFVDGKPLVNEISA"
     gene            complement(1313..1663)
                     /gene="rplT"
                     /locus_tag="PLZ54_07075"
     CDS             complement(1313..1663)
                     /gene="rplT"
                     /locus_tag="PLZ54_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOJ66724.1"
                     /translation="MPRSVNHVASRKRRKRILNLTKGYFGSRKNVWTVAKNTWEKGLL
                     YAYRDRKAKKRSFRALWIQRINAAARLEGSSYSRLMGALHKSGIVINRKVLADLAMNH
                     PEAFKAIVQKAKNA"
     gene            complement(1750..1947)
                     /gene="rpmI"
                     /locus_tag="PLZ54_07080"
     CDS             complement(1750..1947)
                     /gene="rpmI"
                     /locus_tag="PLZ54_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOJ66725.1"
                     /translation="MPKKKTNSGAKKRFTLTGTGKIKRKHAFKSHILTKKTKKQKRNL
                     THSALVHKANLESVKELLNLK"
     gene            complement(2005..2532)
                     /gene="infC"
                     /locus_tag="PLZ54_07085"
                     /pseudo
     CDS             complement(2005..2532)
                     /gene="infC"
                     /locus_tag="PLZ54_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772413.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
     gene            2921..3493
                     /locus_tag="PLZ54_07090"
     CDS             2921..3493
                     /locus_tag="PLZ54_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOJ66726.1"
                     /translation="MKEFDLKGALRNGFGKKASKLERSSEKVPCVLYGNGENVHFTVS
                     EVDVLKLIVNPDVFVVNLDIEGKKCKAIVKEVQFHPVTDKILHIDFFMISEAKPVVVE
                     LPVKLEGLAEGVKAGGRLSLEMRKLKVKGIYTKLPEKVVIDVSSLGLGKTIQVGSLSF
                     NDFEILNAKNAVVAQVKVTRASYAAAAGGK"
     gene            3614..4177
                     /gene="pth"
                     /locus_tag="PLZ54_07095"
     CDS             3614..4177
                     /gene="pth"
                     /locus_tag="PLZ54_07095"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOJ66727.1"
                     /translation="MKFLIVGLGNIGSEYENTRHNIGFRILDAFAEASNVFFTDQRYG
                     AVSEVKLKGHTLILLKPSTFVNLSGNAVRYWMQKEKIDVEHLLVVVDDIALPFGTIRM
                     KPKGSDAGHNGLKNIQEILGHNHYARLRFGIGNDFPRGRQVEYVLGKWTEEEAAQLPE
                     RIEKAIEVIKSFCLIGVEKTMALYNNK"
     gene            4190..4603
                     /locus_tag="PLZ54_07100"
     CDS             4190..4603
                     /locus_tag="PLZ54_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOJ66728.1"
                     /translation="MKSEVRIDKWLWAVRLFKTRSLAAEACKKGKILIHGQPVKASHS
                     IRIGDVVGVKRNPVLFSYQVLALTENRLNAKLLPEYLLDVTTPDQRELFELGKLNNSG
                     RIRGSGRPTKKERRDLEDFVEPFFLDDDEDGWEDL"
     gene            4779..5417
                     /locus_tag="PLZ54_07105"
     CDS             4779..5417
                     /locus_tag="PLZ54_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOJ66729.1"
                     /translation="MKILKNIVFDFGGVLIDWNPRYLYRNVFETEDEMEYFLEHICPY
                     DWNLMQDAGRLLAEATLSKQKEHPEYSNEIALYYGRWIEMIGGIFEENVKLLKPLKEK
                     YNLYGLTNWSAETLPLAQAKYDFFDLFDGIVVSGAEKIVKPDPELYRILIDRYRINPQ
                     ETLFIDDNPQNIQTAERLGFHTLYLTPQINLEQELKNRGIIDCWKKEFRSEK"
     gene            complement(5437..6345)
                     /locus_tag="PLZ54_07110"
     CDS             complement(5437..6345)
                     /locus_tag="PLZ54_07110"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOJ66730.1"
                     /translation="MNKQTHKRTPLPSTKKGQEMKVSENALLLDFLLKSMNHKSRNSV
                     KSLLKHGQIVVNGKTITRYDYPLKLGDIITVGNHQSIVEKNIPQMRILYEDDDIIVID
                     KEAGLLTITTGNGIDITAVSLLKDYVQKQSPYNKIYTVHRLDQMTSGVLVFAKNSEAQ
                     HQLRDNWHNMVTKRTYVAVVEGKMDESAGKISSWLTENKKNYMVYSSPVDNGGKLAIT
                     RYKVLQTSDQFSLLELELETGRKNQIRVHLKYLGHPIVGDRKYGASTSIGRIALHARV
                     LEFYHPVSGEIMHFETPVPHLFLRLL"
     gene            6744..7994
                     /locus_tag="PLZ54_07115"
     CDS             6744..7994
                     /locus_tag="PLZ54_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66731.1"
                     /translation="MNIHQQIIDNLHSPEELEKIYREHKREFSDFFKNNKGNYDSELY
                     RFWQVRLAAETSHSRHKALKSDIIFLMLVSLFSGLLLKLPDFFAQLDKNSFYLRNLSL
                     ILFNALILYAFWQNKLFKKHEMLIYSISIVALAIYVNLLPVDKSDAVRLALLHVPVLL
                     WYLFGWVLVSFDRNNLNKRIGYIRYSGEYLVMTGLILIGGALLTALTINMFSAIGMNI
                     ENFWMEYVVIFGGAAVPVVSAFLIHIYPDVTGKITPVLARIFSPLALVTLAVYLVFLL
                     MGNSNVMKDRNLLMALNVVLFAVLVLVVFSISEIDQSKKKNAGVFVLFLLTLLAILVN
                     VVALTAIVSRLFNGFTPNRTVVLGFNVLIFINLILLLPKLFRSFSHPEMLEEVEQSVA
                     RYLNVYFLWTLMVVFVLPVLFRFR"
BASE COUNT         2390 a         1391 c         1606 g         2728 t
ORIGIN      
        1 attgcataca caaacacgaa tgaatatatt cagacatgtg ggttccatca gacattaaaa
       61 aaataaatta tattatgatg aaaacaaaag ttatcttatt tgacgcaaaa tcatacgacg
      121 aaattttttt taaagaaaaa aatcaacaac atggtttcga tattaaattt caaaaaagtc
      181 atttaagtaa agacaatgcc attttgacga aaggagccga tgtggtttgt atttttgtca
      241 atgcggaagt tgatgcggaa gtaattgata ttttagtaga aaatggtgta aaactgatcg
      301 cactacgctc ggcaggctac aacaatgttg atttaaaagc tgcaaaaggc aaaataaagg
      361 ttgtgcgtgt gccggcctat tcccctcatt ccatagcaga gcataccatt gccttgatgc
      421 ttacgctgaa ccgaaaaatt cataaagctt acactcgaac acgtgaggga aattttgcat
      481 tggaaggatt aatgggattt gaaatgaacg gtaaaacagc cggaataatc ggaacgggaa
      541 aaataggaaa aattgtaatt caaattctga aaagcatggg aatgaacgta cttgcctatg
      601 atttgtatcc cgatacgcaa tttgccgaaa caaacggtat tcgatatacc caactagatg
      661 aattgtatgg actttccgat gttatttctt tacactgtcc gttgaccaaa gaaacggaat
      721 atcttattaa tgaaaaatcc atccataaaa tgaaagaagg cgtaatgatt atcaatacgg
      781 gtagaggaaa gctaattaag accaaagctc tgatagatgc actgaaaagt aaaaaaatag
      841 gttatgccgg cttggatgtg tacgaagaag aaggagatta tttttacgag gatcattccg
      901 atattgtgat cgacgacgac attctggcac gtcttcttac tttcaacaat gtaatcgtta
      961 catctcatca agcatttttt accaaagaag ctctttcgga aatcgcccgt atcacgctgc
     1021 aaaacattca ggattttgtg gatggaaaac cgctggtgaa tgaaatttcg gcttaactgg
     1081 cttttattac agtacaaaat aatgaatatc agttaattgt ataaatttaa tattttataa
     1141 aatttactgt ttatcagcta ttaattgaaa ttttaccgag acattgaaac ccggagtttt
     1201 gcggattttc atttcaatat ccaataaaaa aggctgtcct acttctagtc agcctttttt
     1261 acttaaaatt ttccaataaa ccccgtaaag gttttttaca agggaaaacg gtttatgcat
     1321 tttttgcttt ttgtacgata gctttgaatg cttcgggatg attcatggcc aaatcggcga
     1381 gcaccttgcg gttaattacg ataccggact tgtgtagtgc tcccatcaat cgggaataag
     1441 atgaaccttc gagccgtgca gcagcgttga tacgttgaat ccataaagcg cggaaacttc
     1501 tttttttggc cttgcggtcg cgataagcat agagcaagcc tttttcccaa gtgtttttgg
     1561 caacagtcca cacattcttg cggctgccga aataaccttt tgtcaggtta agaattcttt
     1621 ttcttctttt gcgcgaagca acatgattaa cagatcttgg cataatcgat aaaatttatg
     1681 aatgtcagcg ccgtcttttt tcaacggact ttgggctaag cattctgatt actgaataaa
     1741 aaaattactt tattttaaat ttaataactc tttcacactc tccaaattag ctttgtgaac
     1801 gagagcgctg tgggttaagt tacgtttttg tttttttgtc tttttagtca aaatatggct
     1861 cttaaaagcg tgtttccttt ttatttttcc tgttccggtc aacgtaaatc ttttctttgc
     1921 tccagagttt gtctttttct ttggcatttc gcttaaatat ttagtaaatt gaataattga
     1981 attaaaataa tgtctttatt ttgatttttt tggcgataac tgtattgtca ttctttttcc
     2041 ttccaaaatc gtcaattgct ccactttggc atattcctcc aaatcgttgg cgaaacgcag
     2101 cagcagcact tctccctgtt ctttaaaaag gatggaacga cccttgaaaa atacataagc
     2161 tttcactttg caaccttctt tcagaaagtt ttgtgcgtgt ttcagcttga tgttgtaatc
     2221 gtgctcgtcg gtttgtggtc caaaacgaat ttcctttact accactttag ccgttttggc
     2281 tttcagttcc ttttcacgct ttttgagctg atatagaaat ttctgataat caatcacctt
     2341 gcatacagga ggatctgctt tggctgaaat ttcaaccaaa tccaaatttt gttcttcggc
     2401 tatttctaaa gctcgctgta taggataaat tcccggttcg acattttcgc caaccagacg
     2461 aacttctttt actcccttaa tattttcgtt gattctatgg agctgttctg ttgacttacc
     2521 ttgttgtttc acagataatt gtttagctat aactgttcct cctcgttatt aattttcaaa
     2581 cgtattattt taaaatttta agactgcaaa tataaatgtt ttttttgaat tttccattat
     2641 tctgtcgaat atttttaccg acggagtgct gataaatcaa atctaacttt ctaataatgt
     2701 ataaattaat aataatgacc agttttttgt tctttatttt gattgtaatt gataggttta
     2761 ttcgaataat ttgggtagtt tttatattgt tttacatcaa aaaattaact acctttgcag
     2821 cacttttttt gaaaaaagaa agtgtgatgg gaaattaagc tactgatcac atgttttttt
     2881 aatgcttaaa ttttgagtaa cacattaatt taaagttaaa atgaaagaat ttgatttaaa
     2941 aggggcctta cgtaacgggt ttgggaaaaa agccagcaag ttggaaagaa gttctgaaaa
     3001 agttccgtgt gttttgtacg gaaacggaga aaatgttcat ttcacagttt ctgaagtgga
     3061 tgttttaaag ttgattgtta atcctgatgt atttgtggta aacctcgata tagaagggaa
     3121 aaaatgtaaa gctattgtta aggaagttca atttcacccg gtaacggaca aaattttgca
     3181 catcgacttc tttatgattt ctgaagctaa accggtggtt gtcgagttgc ccgttaagct
     3241 ggaaggtttg gctgaaggtg tgaaagccgg tggacgtttg agtcttgaaa tgagaaaatt
     3301 gaaagtgaag ggaatttata ccaaacttcc tgaaaaagtg gttattgatg tttcttcgct
     3361 tggattgggc aaaaccattc aggttggaag tctttccttt aacgattttg aaattctgaa
     3421 tgcaaaaaat gccgttgttg cacaggttaa ggttaccaga gcttcttatg ctgctgcagc
     3481 aggcggaaaa taatttttgt tttaatggtt tgtcgttttt cttttaaaag aaagaattcg
     3541 gcaaatgtaa caaccgattt gttcgtaaat ttttcagaag ttcaataatg aaccgatttg
     3601 aaaataattg tatatgaagt ttttaattgt aggtttgggc aatattggaa gtgaatacga
     3661 aaatacccgc cacaatatag gttttaggat attggacgct ttcgctgagg cgtccaatgt
     3721 tttttttacc gaccaacgtt atggagctgt cagtgaagtg aaattaaaag gtcatacgct
     3781 cattttgttg aaaccttcca catttgtcaa tctgagcgga aatgccgtga gatactggat
     3841 gcaaaaggaa aaaatcgatg ttgaacattt actggtagtg gtggacgata ttgctcttcc
     3901 gtttggcaca attcgtatga aacccaaagg ttcggatgcc ggacacaatg gcttgaaaaa
     3961 tattcaggaa atactgggtc ataatcacta tgcccgattg cgtttcggaa taggcaatga
     4021 tttccctcgt ggcagacaag ttgaatatgt acttggaaaa tggacggaag aagaagcggc
     4081 ccaattgccc gagagaattg aaaaagcaat agaagtaata aaaagtttct gtttgattgg
     4141 ggttgaaaaa actatggcat tgtacaacaa caaataattt cctctgttta tgaaatcgga
     4201 agtgcgcatc gacaaatggc tgtgggctgt caggctgttt aaaacccgtt cgctggctgc
     4261 cgaagcctgt aaaaaaggga aaattctgat tcacgggcaa ccggtaaaag cttcgcattc
     4321 gattcgtatt ggcgatgtgg ttggtgtgaa acgaaatccc gtactttttt cttaccaagt
     4381 tctggccttg actgaaaaca gattgaatgc caaattgctg cccgagtatc tgcttgatgt
     4441 tactactccc gatcaacgcg aattgttcga actcggaaaa ctgaataatt caggaagaat
     4501 acgcggcagt ggacgtccta caaaaaagga acgtcgcgat ttagaagatt ttgttgaacc
     4561 tttcttcctc gacgatgacg aagatggctg ggaagatttg taaacatact tacctgaaat
     4621 tttttcaatc cgtttaatag gttgcttttt attctttcct attcactaat ataccaattt
     4681 ttatatttta ataaaccctg ctgttagggc gaatgaaaac agaattctgt tttcaatttt
     4741 taagatttac ttttctataa aattcaaact tacgtcatat gaagattctc aaaaatatcg
     4801 ttttcgactt tggaggtgta cttattgact ggaaccctcg ttatttgtat cgaaacgttt
     4861 tcgagactga agatgaaatg gaatattttc tcgaacacat ttgtccatac gactggaatc
     4921 tgatgcagga tgccggccgt ttgctcgccg aagctacgtt gtcgaaacaa aaagaacatc
     4981 ccgaatacag caacgaaatt gcgttatatt atggtcgatg gattgaaatg ataggtggaa
     5041 ttttcgaaga gaatgtgaaa ttactgaagc cgctgaaaga aaaatacaat ttgtatggac
     5101 ttacgaactg gtcggccgaa acgcttccgc ttgctcaggc taaatacgat ttttttgatt
     5161 tgtttgacgg aattgttgta tcgggtgcgg aaaaaattgt aaaaccagat ccggaactct
     5221 atcggatatt gatcgatcgt tatcgaatca atcctcagga aacactgttc atcgacgata
     5281 atcctcaaaa tatccaaaca gccgaacggc tcggatttca tacgttgtac ttgacgcctc
     5341 aaatcaattt agaacaagaa ctgaaaaata gaggcattat cgattgttgg aagaaggaat
     5401 tccgctcaga aaaataatta taggcggaag cttctttcat agcaaacgta aaaaaagatg
     5461 gggtacaggt gtttcgaaat gcatgatctc acccgaaaca gggtgataaa attccaacac
     5521 gcgcgcatgc agagcgatgc ggcctataga tgtggatgct ccatatttgc ggtcgccaac
     5581 gatgggatgc ccaagatatt tgagatgaac ccttatctga ttttttcgtc ctgtttcgag
     5641 ttccaattcc aatagcgaaa attggtcgga tgtttgcaaa actttatatc ttgtgatagc
     5701 cagctttccc ccattatcca caggactgga atacaccatg tagttttttt tgttttcggt
     5761 cagccaactc gagatttttc ccgccgattc atccattttt ccctctacaa cagccacata
     5821 agtgcgtttg gtaaccatat tgtgccaatt gtcgcgcaac tgatgctgag cttcgctgtt
     5881 tttagcaaaa accagcactc ccgaagtcat ttgatcgagc cgatgaaccg tgtaaatttt
     5941 attgtaggga gattgttttt gtacataatc cttaagcaac gaaactgccg taatatctat
     6001 tccattcccg gtggtgatgg taagcagtcc ggcctctttg tcgatgacga tgatgtcatc
     6061 atcttcataa agaatacgca tttggggtat attcttctca acgatacttt gatgattgcc
     6121 aactgtaatt atatcgccca attttaacgg ataatcatag cgcgtaatgg ttttgccgtt
     6181 cacaactatt tgaccatgtt tgagtaatga ttttaccgaa tttcggctct tatggttcat
     6241 gcttttcagc aaaaaatcga gcaaaagggc attttcgctc actttcattt cttgtccctt
     6301 tttcgttgat ggaagaggag tccttttatg cgtttgtttg ttcacttcga taagttgttt
     6361 tcgtaatctt ttataaaccc atgagccatt cagttagcca ttgcctgacg attattttga
     6421 agaatgatgc aacgtcgatc gaaatcattt tagataattc cgactttttc tattattttt
     6481 tcaatgcaat cacagtttta atcctgtcgc aaaattacaa aaattatagc attgcatttc
     6541 ccttgaagaa tgaaggtgcc aaaaaatttt tcgagtatat ttttaatttt ttttaaaatt
     6601 tagtggaaaa gtgtacgttt atttcttaaa tagtcaacta ctgtgtgaaa ataattggaa
     6661 tttgcacaag gcgacaatac agtaaattga ttatcaattt ttttctgttt tgtacaaaaa
     6721 accatttctt tcaaattact taaatgaata tacatcagca aattatcgac aatctgcatt
     6781 ctccggaaga gttggaaaaa atttacaggg aacacaaaag ggaattttcg gattttttta
     6841 aaaacaacaa gggaaactac gattcggagc tttatcggtt ttggcaagta cgtttggcgg
     6901 cagaaacctc acatagtcgc cataaagcgc tgaaatcgga tattattttt ttgatgctcg
     6961 tttcactatt cagtggattg cttttaaaac taccggattt tttcgcacag ttggataaaa
     7021 attcttttta tttgcgtaac ctttcgttaa ttcttttcaa tgctttgatt ctttatgctt
     7081 tctggcaaaa caaactgttt aaaaagcatg aaatgttgat ttattcaatt tcgatagttg
     7141 ctttggcaat atatgtaaat ctgcttccgg tggacaaaag tgatgctgta cgtcttgcct
     7201 tgttgcatgt tccggtatta ctctggtatc tgtttggctg ggtgcttgtt tcgtttgatc
     7261 gcaataactt gaacaaaaga atcggttata ttcgttacag tggagaatat ttggtgatga
     7321 caggattaat cctgatagga ggggcattgc tcaccgcttt gactatcaac atgttttctg
     7381 ctattggaat gaatatcgaa aatttttgga tggaatatgt tgtaattttt ggcggagctg
     7441 ccgtgcctgt tgtttcggct tttctgatac atatttatcc ggatgttacc gggaaaatta
     7501 caccggttct tgcccgcatt ttttcaccgc tcgcattggt tacgctggct gtttatttgg
     7561 tttttctttt gatgggaaac agtaatgtta tgaaagacag aaatttattg atggcactga
     7621 atgtcgttct ttttgccgtt ttggtgttgg ttgttttttc gatttcggaa atcgatcaat
     7681 cgaaaaagaa aaatgcaggc gtttttgtgt tgtttctgct gactcttttg gcaattttgg
     7741 tcaatgtggt agccttaacc gccattgtct cccgactttt caacggattc acaccaaaca
     7801 gaaccgtcgt tttaggtttc aacgtattga ttttcattaa tttgattctt ttgttgccaa
     7861 aactgtttcg ttccttttct catcctgaaa tgctcgaaga agtggaacag tcggttgccc
     7921 gatatttgaa cgtgtatttt ctctggacac tgatggtggt atttgtattg ccggtgttgt
     7981 tcaggtttcg gtgagttgga caatatcaag gtcaaaattt caaaattaat tttttttaaa
     8041 tcattgcttt gcgtgaatag aattgttgtg tatatttgtg aaattattaa aaaaatagca
     8101 agctggtaga gaaag
//
LOCUS       DAOSCH010000091         8065 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5802_length_8065_cov_6.312609, whole genome shotgun sequence.
ACCESSION   DAOSCH010000091 DAOSCH010000000
VERSION     DAOSCH010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5802_length_8065_cov_6.312609"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /gene="sufC"
                     /locus_tag="PLZ54_07120"
     CDS             <1..404
                     /gene="sufC"
                     /locus_tag="PLZ54_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319031.1"
                     /note="part of SUF system involved in inserting
                     iron-sulfur clusters into proteins; in Escherichia coli
                     this protein forms a complex with SufBD; the SufBCD
                     complex stimulates the cysteine desulfurase SufS in
                     conjunction with SufE; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOJ66732.1"
                     /translation="RLMREKKELVEMDNKLTSRSVNEGFSGGEKKRNEIFQMAMLEPK
                     LAILDETDSGLDIDALRIVANGVNKLKRPDNATLVITHYQRLLDYIIPDFVHVFYDGR
                     IVKTGDKNLALELEEKGYDWIKKEVETPVEQ"
     gene            439..1776
                     /gene="sufD"
                     /locus_tag="PLZ54_07125"
     CDS             439..1776
                     /gene="sufD"
                     /locus_tag="PLZ54_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOJ66733.1"
                     /translation="MEQQYIDFYKESHTLIKQHSAALMNNFRDAAFEKFERLGFPTSK
                     LENYKYTDMSAPLSIEYGLNINRLQFQINPYDVFKCDVPAIHSYLFFVLNDTFYPVNS
                     TAKQHLPEGVIIESLRTAAENYPQLVEKYLGKLSNEKNDGWIAFNETFAQDGFFMYVP
                     KNVKLSKPVQLINIMRSDVDFMANSHNLIILEEGAKAQLLVCDHTMDNVHFFSNRLTE
                     VFAGKNAVYEHYKLENTNILNTNVSNLLIHQEDSSNVLTNVITLHNGMTRNGIEMIID
                     GENCESLLCGMVIGDKNQDVDNFTEIVHKKPNSQSRELFKYVLDDSSKGAFAGKIQVA
                     KNAQKTSSFQTNKNILLSKTAKMRTKPQLEIYADDVKCSHGATIGQLDEAAKFYMQSR
                     GISESEARLLLMFAFTADVMDNIRIDALRDRMKLLVEKRLRGELSRCEGCMIC"
     gene            complement(1991..3292)
                     /locus_tag="PLZ54_07130"
     CDS             complement(1991..3292)
                     /locus_tag="PLZ54_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HOJ66734.1"
                     /translation="MNINNLKFETLQVHAGQEVDKTTKSRAVPIYQTSSYVFDDAADG
                     ADLFALRKFGNIYTRLQNPTTDVFEKRMAALEGGISALATSSGQAAEFLALNNIVQAG
                     DNFVATSHLYGGTYNQFKNQFKRLGVDVKFTANDEPETFEKLIDDKTKAIYIETIGNP
                     DLNIPDFEAIAAVADKYDIPLIVDNTFGAGGYLFRPIEHGASIVVESATKWIGGHGTS
                     LGGVIVDSGKFNWGNGKFPAFTEPSESYHGLVFWDAFGADSPFGNIAFNIRARVEGLR
                     DWGCVISPFNSFLLLQGLETLSLRMERHVQNALELARWLQTLSAVEYVNYPGLENNPY
                     HEMAKKYLKKGFGGVLAFKLKGDASMADKFINNLKLISHLANVGDAKSLIIHPATTTH
                     EQLSPEDKIAAGVFPGLLRFSVGIENIDDIKADIEQALSNI"
     gene            complement(3326..4273)
                     /gene="cysK"
                     /locus_tag="PLZ54_07135"
     CDS             complement(3326..4273)
                     /gene="cysK"
                     /locus_tag="PLZ54_07135"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOJ66735.1"
                     /translation="MSKIAKNLTELIGNTPLLQLTHFVSNKGLQANIIAKLESFNPAG
                     SVKDRIALAMIEDAEEKGLLKQGSEIIEPTSGNTGIGLAMIATVKGYKLTLTMPETMS
                     VERRNLLKALGANIVLTPGAEGMKGAIAKAHELHDQNPSSFMPQQFENPANPLKHFHT
                     TGEEIWRDTEGKVDIFVAGVGTGGTISGVGAALKKHNPAIKIVAVEPASSPVLSGGKP
                     GPHQIQGIGAGFVPKNFDPTVVDEIIQVSNDDAIRTSRQLAQQEGLLVGISSGAAAFA
                     ASLLSQRPENKGKNIVVLLPDTGERYLSTALYAFDEYPL"
     gene            complement(4744..7953)
                     /locus_tag="PLZ54_07140"
     CDS             complement(4744..7953)
                     /locus_tag="PLZ54_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ66736.1"
                     /translation="MAIIPLLKAQPTLKPNDYDITKDRLLYTVGYAHLDTEWNWDYPT
                     TINEHILHTMVENFALFEKYPDYVFNFTGSRRYKMMKEYYPQLYNQVKNYVEQGRWKV
                     SGSSVDEGEVNISSSESLVRQVLYGNEFFKNEFGKESFDYILPDCFGFLWNLPSILNH
                     CGLIGFSTQKLTWRSAAGVPFNVGVWKGPDDKGIVAALNATSYTSRIEERLDKNPQWV
                     ERLNQDKEKTGYAFDYRYYGVGDEGGAPRINDVKRAVNSLHNDDSNFKVLLTSSDQMY
                     KDITPEIQAKLPVYSGDLLLIEHSSGSLTSQAFMKRMNRKNELLASSAELASVAADWM
                     GTAAYPFGKLNNGWDLVLGSQFHDILPGTSIPKAYEYAWNDEFIAANCFAEALKNSLS
                     SIAGKMNTQTEGKAIVVYNQVSRARQDVVTALLEYKSLPQTVGVFDKNGKEVPSQIIE
                     RNGNGIKILFLADMPSASIKVYDVREISNAVKQTALSISENSLENEYYKVKVAPNGDI
                     LSIYDKKNTKELLAKPARLEFLAETPDEWPAWNMDWKDRQKPAIDFMDKNAKISVVEQ
                     GPVRVTLKVERAGQNSTITQFYSLAAGEAGKRLEVSNKIDWQSRAVSLKATFPLSVSN
                     KNATYNLGVGTISRGNNEEKKFEVPSKQWFDLTDQSGKYGVTILEDCKYGSDKPDDNT
                     LRLTLLYTPGIHLPQWQRTMYQSTQDWGIHDVKYAVYGHQGDWRKGESAWQAQFFNQP
                     LMAFESTKHDGTLGNSFSMISISRPEVGLMAFKKMESSEGYVIRVNELSGKDIKNAKI
                     TLPGEILQAYEVNGQEKRIGDVAFTKNSIQFDLSHYTIKSFAVKLKKANEAVLPQQPV
                     TIPYNADIMSYDENRDDGVMFDGQSFPAELLPEYVESEGIRFKIGNKQDEQNNAVACN
                     AQTIALPAGNFTKLYLLAAAANDVKADFYVDGNPINLSIQKWTGFVGQFYNRILSLDQ
                     NKVLKMNEPFTKTDNIAWFASHCHNKYPMKNEAYQYSYLYKYEIPIVAGAKNLTLPNN
                     RNIKIVAITVAAPKADNLKTLQAMYDDFENNPTFHLREK"
BASE COUNT         2418 a         1664 c         1481 g         2502 t
ORIGIN      
        1 tgcgtctgat gcgtgagaaa aaggaactgg tggaaatgga caataaactg accagccgtt
       61 ccgtgaatga aggtttttcc ggcggagaaa agaaacgaaa cgaaatattt caaatggcaa
      121 tgctggaacc taaattggca attcttgacg aaacagattc cggacttgat attgatgcgc
      181 tgcggattgt tgccaacgga gtaaacaaac tcaagcggcc tgacaatgcc acacttgtga
      241 taacccatta ccaacgcttg ctcgactata ttattcccga ttttgtacat gtgttttacg
      301 acggacgaat cgtaaaaaca ggcgataaaa acttggcact tgagctggaa gaaaaaggtt
      361 acgattggat aaaaaaggaa gttgaaactc ctgtagagca gtaaaaattc ttcttaacta
      421 tccgaaattt gaaaaatcat ggaacaacaa tatatcgatt tttataaaga atctcatacc
      481 ctgataaagc aacattcggc tgctttgatg aataattttc gcgatgccgc ctttgagaaa
      541 tttgaacggc tcggttttcc cacttcaaag ctcgaaaact ataaatatac cgacatgtcg
      601 gcacctttgt cgatagaata tgggctgaac atcaaccgat tgcagtttca aatcaatcct
      661 tacgatgttt ttaagtgtga tgtgccggct attcattcct acttgttttt tgtgttaaat
      721 gatacttttt accccgtcaa cagcactgcc aaacagcatt tgccagaagg cgtaattatt
      781 gaaagtctgc gaacagctgc agaaaattat ccacaactgg tagaaaaata tcttggaaaa
      841 ttaagcaatg aaaaaaatga cggttggatt gctttcaatg aaactttcgc tcaagatgga
      901 ttttttatgt acgttcccaa aaacgtgaaa ctttccaagc ctgtacaatt gataaacata
      961 atgcgctccg acgtggattt catggccaac agccacaatt taatcattct ggaagaagga
     1021 gccaaagctc agttgctggt ttgcgaccac actatggaca atgtgcactt cttttccaat
     1081 cgtctcaccg aagtttttgc aggaaaaaat gctgtttacg aacattataa actcgaaaac
     1141 accaatatcc tcaacaccaa tgtttcaaac ttactgattc atcaggaaga ttcgtcgaat
     1201 gtactgacaa acgtcattac tcttcataat ggcatgacac gcaacggcat cgaaatgata
     1261 attgatggag aaaattgtga aagtttgctt tgtggcatgg tgattggcga taaaaatcag
     1321 gatgtggata atttcactga aattgttcac aaaaaaccaa attctcaaag tcgcgaattg
     1381 tttaaatatg ttttggatga ttcttccaaa ggagcatttg caggaaaaat acaggttgca
     1441 aaaaacgccc aaaaaacttc atcctttcaa accaacaaaa atattctcct cagcaaaact
     1501 gccaaaatgc gaaccaaacc gcaactcgaa atctatgccg acgatgtgaa atgttcgcat
     1561 ggcgctacca tcggacaatt ggacgaagca gccaagtttt acatgcaatc gcgtggtatt
     1621 tcggagagcg aagcacgttt gctgctaatg tttgccttta ctgcggatgt gatggacaat
     1681 atccgtatcg atgctttgag agacagaatg aaattattgg tagaaaaacg tcttcgcggt
     1741 gaactttccc gatgtgaagg ctgcatgatt tgttgacttt ttcggtgaaa ttattgtaat
     1801 tctctgtttt ttgtaaaata tttttgtcga ttttctccgg tatttttttg tgttgattaa
     1861 taaatttggg tcaatttttt tatggggaac aatttatgtt ttctgataac acatcgattt
     1921 acaacttttc atttattaaa aaaggctgcc ggttttgttc caacagccat aaggaattaa
     1981 gaaataaaaa tcaaatgttg gaaagggctt gctcaatatc ggctttgata tcgtcaatat
     2041 tttcgattcc cactgaaaaa cgcagtaatc ccggaaaaac tcccgctgca atcttgtctt
     2101 cgggcgaaag ttgctcgtga gttgtggtag ccggatgaat aataagagat tttgcatcgc
     2161 ccacattggc caaatggctt atcagcttta agttgttgat gaatttgtct gccattgaag
     2221 catcgccttt caatttgaaa gccaatacgc cgccaaaacc ttttttcagg tatttttttg
     2281 ccatttcgtg ataaggatta ttttccagac cgggataatt aacatattcc acagccgaca
     2341 atgtttgtaa ccagcgagca agttccaacg cattttggac atgtcgttcc attctcaaag
     2401 acaaagtctc caaaccctgc agcaacaaaa acgaattaaa aggactgata acgcatcccc
     2461 aatcgcgaag accttctaca cgtgctctta tattgaaagc aatattccca aatgggctat
     2521 ctgctccgaa agcatcccaa aaaaccaatc catgataact ttcagaaggt tcggtaaaag
     2581 ccgggaattt gccatttccc caattaaact taccactgtc cacaattaca cctcccagcg
     2641 aagtaccatg tccgccaatc catttggtag cgctttctac cacaatggaa gctccatgtt
     2701 ctatgggacg gaaaagatag ccgcctgcgc caaaagtatt gtccactatc agcggaatat
     2761 catatttatc ggctacagca gcaattgctt caaaatcggg gatattcaaa tcgggattgc
     2821 caatggtttc gatataaatg gcttttgttt tgtcgtcaat caatttttca aaagtttccg
     2881 gttcgtcatt tgccgtaaac ttaacgtcca cacccagacg tttaaattga tttttaaact
     2941 gattgtaagt tccgccgtaa agatgagaag ttgctacaaa attatctcca gcctgtacaa
     3001 tattgtttag agcaagaaat tctgcagcct gtcccgatga agttgccaat gcgctaatgc
     3061 ctccctcgag tgcagccata cgtttttcaa acacgtcggt agtaggattt tgcagacggg
     3121 tatagatatt tccaaatttt ctcaaagcaa aaaggtcggc tccatcggct gcgtcgtcga
     3181 aaacgtagga agaagtctga taaatgggta ctgctcgcga tttggtagtt ttgtccactt
     3241 cctgaccggc atgaacctgc aaggtttcaa attttaaatt gttgatattc atattgctaa
     3301 ttatttagat ttcgattctt gaaatttata aaggatattc gtcaaaagca taaagtgcag
     3361 tggaaaggta tctttcgccc gtatcgggca acagaacgac aatgtttttt cctttgtttt
     3421 cgggtcgttg agacaacaaa gaagcggcaa aagctgccgc acccgacgaa attcccacca
     3481 gcaagccttc ctgttgagcc aattggcgtg aagttcgaat ggcgtcatca ttgctaacct
     3541 gaataatttc atctaccacc gtcggatcaa aatttttggg tacgaacccg gctcctattc
     3601 cctgaatttg atggggtccg ggtttccctc ccgaaagtac gggagaactt gcaggttcga
     3661 ccgcaacaat ttttattgca ggattatgtt ttttcaacgc agctcctact ccacttatcg
     3721 ttcctccggt tcccacaccg gcaacaaaaa tatcgacttt cccttccgta tcacgccaaa
     3781 tttcctcgcc tgtggtatga aaatgtttca atggattggc aggattttca aactgctgcg
     3841 gcataaacga agatggattc tgatcatgta attcatgcgc tttggcaata gcacctttca
     3901 ttccttcagc tccgggcgtt aaaacaatat tggcacccaa agccttcagt aaatttcttc
     3961 gttcaacact catggtttcg ggcatggtaa gggtaagttt gtaacctttc accgtagcaa
     4021 tcatagccaa accaatacct gtatttccgc tggtaggttc gataatttca gaaccttgtt
     4081 ttaaaagtcc tttttcctct gcgtcttcaa tcatggctaa ggcaattctg tcctttacgc
     4141 ttcctgccgg attaaatgac tccagcttgg caataatatt ggcttgcaat cctttatttg
     4201 aaacaaaatg tgttaattgc aacaaaggtg tattgccaat aagttcagta agatttttag
     4261 caattttcga cataaacttt tgatttttaa ttaatttaca aaatcttttt taccgaaaaa
     4321 aatatctgac taaagatttt gtttttcttt tggcaaaggt acaaaacaac taaaaacaag
     4381 tcaatcacaa gaatgggtta atttaatcta ccacgatgat ggtatagtca aaatttcaaa
     4441 taacaataat ggagaaaatt aggattcaaa tttatataat aatttgtaaa tcaagaatat
     4501 aattaatttg agaaacaaac gaaaagaatt tccattttgc ttattatgaa acccatatgt
     4561 attgcataca caaacacgaa tgaatataat attcagacat gtgggttcca tcagaccttt
     4621 aaaaaataaa ttatattctg atgaaatttc tctgtaaatc ttttgacaag ttttaaagag
     4681 aaatttatca attcggtttt tgtgctattt atcgaaatta tcaatagcaa tcacctttaa
     4741 aaattatttt tctctcaaat gaaatgtcgg attattctcg aaatcgtcat acattgcctg
     4801 aagagttttt aaattgtcag cttttggagc agccaccgta atcgcaacaa tttttatatt
     4861 tctgttgttt ggtaaagtca aattcttggc tccggcaaca attggaattt cgtatttata
     4921 caaatagctg tactgataag cttcgttttt catcggatat ttgttgtggc aatgcgaagc
     4981 gaaccatgcg atattatccg tttttgtaaa aggctcattc attttcagca ctttattctg
     5041 atccaaactc agaatacgat tgtagaattg accgacaaaa cctgtccatt tttgaatcga
     5101 caaattgatt gggttaccat ccacataaaa atcggctttc acatcatttg cagcagcagc
     5161 caaaagataa agtttggtaa aatttccggc aggcaaagca atggtttgag cgttgcaagc
     5221 aacagcatta ttttgttcgt cctgtttatt accaattttg aaacgaatgc cttcgctttc
     5281 gacatattcc ggcaaaagtt cggcaggaaa cgactgacca tcgaacatga ctccgtcgtc
     5341 gcgattttcg tcgtaactca ttatatccgc attgtaagga atagttaccg gttgctgtgg
     5401 caatacagct tcatttgctt tcttgagttt gacggcaaaa ctttttatcg tataatgact
     5461 taaatcgaat tgaatgctgt tttttgtaaa agctacatcg cctattcgtt tttcctgtcc
     5521 gttcacttca taagcttgca gtatttcgcc cggcaatgtg attttcgcat tttttatatc
     5581 ttttcccgac aattcattca cacgaataac gtatccttcg ctcgattcca ttttcttaaa
     5641 ggccattaaa cctacttcgg gacgactgat cgaaatcatc gagaaactgt tgcccaatgt
     5701 tccatcgtgt ttggtggatt caaaagccat taaaggctga ttgaaaaatt gagcttgcca
     5761 tgctgattct ccttttctcc aatcaccttg gtgtccataa acggcgtatt tcacatcatg
     5821 aataccccaa tcctgagtac tttgatacat tgtcctctgc cactggggta aatgaatacc
     5881 gggcgtgtaa agtaaagtca gacgcaaggt attgtcatcc ggtttgtcgg aaccatattt
     5941 gcaatcttcc aaaatcgtta cgccatattt tcctgattgg tcagtcaagt cgaaccattg
     6001 tttagaagga acttcaaatt ttttctcctc attatttcct cgagaaatgg taccaacacc
     6061 taaattgtag gttgcatttt tattggaaac ggatagagga aaagttgctt tcaaactgac
     6121 agcacgtgat tgccagtcaa tcttgttgct cacttccaaa cgtttacctg cttcaccggc
     6181 agccaaactg tagaattgcg tgatggtcga attttgtcct gctctttcca ctttcaaagt
     6241 tacccttacc ggcccttgtt cgacaacgga tatttttgca tttttatcca taaagtcaat
     6301 agccggtttt tgtcgatctt tccagtccat gttccatgcc ggccattcgt cgggtgtttc
     6361 tgccaaaaat tcaagacgag ccggttttgc caacaattct ttcgtatttt ttttatcata
     6421 aatactgaga atatcgccat tgggagctac tttcactttg tagtactcgt tttcaagtga
     6481 attttcggaa atagaaagtg cagtttgttt tactgcattt gaaatttcac gtacatcata
     6541 cacttttatg cttgcagaag gcatgtcggc caaaaacaaa attttaatac catttccatt
     6601 tctttcaata atttgcgaag gaacttcctt tccattttta tcgaaaacac cgacggtttg
     6661 tggtaacgat ttgtattcca atagagcggt aacaacatct tgtcttgccc gacttacctg
     6721 attgtaaact acaatggctt ttccttcagt ttgtgtattc attttaccgg caatggaact
     6781 caggctgttt ttgagcgctt cagcaaaaca attggcagca ataaattcat cattccatgc
     6841 atattcatat gcttttggaa tagacgttcc cggcaaaatg tcatgaaatt ggcttcccag
     6901 caccaaatcc caaccattgt ttagtttacc gaacggataa gctgctgttc ccatccaatc
     6961 ggcagcgaca cttgcaagtt ctgccgacga agccaaaagt tcgtttttac ggttcattct
     7021 tttcataaaa gcttgagaag tcaatgaacc cgaactgtgc tctatcaaaa gtaaatcgcc
     7081 cgaatatacc ggaagttttg cctgaatttc aggcgtaata tctttgtaca tttgatcgga
     7141 agaagtcaac aaaaccttaa aattactgtc gtcattgtgc aaactgttga cagccctttt
     7201 cacatcgttt atccgcggtg ctccgccttc atcgccaacg ccataatagc ggtagtcaaa
     7261 agcataaccg gttttttcct tgtcctgatt cagacgttcc acccattgag gatttttatc
     7321 gagacgctct tcaattctgg aagtatagct ggttgcattt aatgcagcaa caataccttt
     7381 gtcgtcaggg cctttccaaa caccaacgtt aaaaggaacg ccagcagcag aacgccaagt
     7441 gagcttttga gttgaaaatc caatcaatcc gcaatgattc agaatggaag gcaaattcca
     7501 taaaaatccg aaacagtccg gcaatatgta atcaaaactt tctttcccaa actcattttt
     7561 aaaaaattca tttccataga gcacttgtcg aacaagtgat tctgaagaag aaatattgac
     7621 ttcaccttcg tcaaccgaag aaccggatac tttccaacgt ccttgttcaa cataattttt
     7681 tacctgatta tacaattgag gataatactc tttcatcatt ttataccgtc gggaaccggt
     7741 gaaattgaac acataatcgg gatatttttc gaagagagca aaattttcga ccattgtatg
     7801 taaaatatgc tcattaatgg ttgttggata atcccaattc cactcggtat ccaaatgagc
     7861 ataacccacc gtgtacaaaa gtctgtcttt tgttatgtca taatcatttg gttttaacgt
     7921 tggctgagcc tttagcaatg gaattattgc caaacaaatc aaaattgtaa ataattgttt
     7981 tttcggctca cctgcaaaaa tatgtgtcta tagaaaaaga aaagtatctt tgtgaaaaaa
     8041 tactttttat atgtccactt acgaa
//
LOCUS       DAOSCH010000092         7963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5891_length_7963_cov_4.942337, whole genome shotgun sequence.
ACCESSION   DAOSCH010000092 DAOSCH010000000
VERSION     DAOSCH010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5891_length_7963_cov_4.942337"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..2420
                     /locus_tag="PLZ54_07145"
     CDS             48..2420
                     /locus_tag="PLZ54_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOJ66737.1"
                     /translation="MKNKILFSFLCTSLFFSCQPSLHYAPDGENNFNLNSLLTLEIGE
                     DTIYLQDFILNPTEIDSITSSSSWLQWKLSNDKKLLYVKVVPEMENFVDLKIWLNGTP
                     YSIPCRKTDKTDFVFTYRPQGKAYRKVQIAGQMNDWVPNFTPDLQLNDSSYYQVTLHL
                     SPGTYLYQMVLDGEWNYDVNNPLKIDNGRGKFNSILKVEGQQSLLPELYTDAYSDSKI
                     TLLSVNKVNNIFVYWQNYRLPDMFVKKQENRIIFDIPSEAHSLERSFIRVWVSNDFGV
                     SNDVFIPLHKGKVLTDSKEITRSDKASQIIYFLMVDRFKNGNKDNDHPLRRADVHPKV
                     DFWGGDLAGIRQKIDDRYFTDLGVNTLWISPVNQNPTTPYGFYAPVPTKFSGYHGYWP
                     ISSSKVDFRFGTNNELKNLVVHAHSNNINILLDYVAHHVHEEHPLYKEHPEFFTSLYL
                     PDGTLNTERWDDQRLTTWFDTFMPTLNTTDPKVIDLMTDSALFWLKEFQLDGFRHDAC
                     KHISNDFWRELTLKIKRQNHGKNLYQIGETYGSPQLIGSFLGTGMLDGQFDFNVFDEA
                     SVAFAGIGGNSLSRVNDVLQNSFKVYGHHNLMGYISGNHDRPRFMAYASGDLKYGEDT
                     KLAGWKREIGISDSTAYDKMFLLHTFNLTIPGIPVIYYGDEIGMTGANDPDCRRMMRF
                     DHWNKREQNLWKKVSTLTHLRLQHPVLIYGDFVSLQANNDSWVYARKYFDKVAIIFIN
                     NAPKSQHFEINLPSIMTNTIYQAVFQHAFTEVNGTINIDLPAYSAEILLN"
     gene            2435..2950
                     /locus_tag="PLZ54_07150"
     CDS             2435..2950
                     /locus_tag="PLZ54_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007480405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP19 family protein"
                     /protein_id="HOJ66738.1"
                     /translation="MIQIPSSELQQALEKDNKEFLRTIANTYLTAIGGNLNTSNMQLL
                     NGWQHVLLSWYFFSTEMDEGGFVQLIQNGYGGYIFDNPFAKALRLMGARELSKLIYRA
                     KKIYDENKTTLERDTSEEEFNAMYVDFEQFDELEEEYFDMEEEQLEIISAYVKGHLSD
                     FTEILANQVNF"
     gene            3124..4146
                     /gene="recA"
                     /locus_tag="PLZ54_07155"
     CDS             3124..4146
                     /gene="recA"
                     /locus_tag="PLZ54_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOJ66739.1"
                     /translation="MAEKTTEEQKKPSSEKIKALQLAMEKIEKDYGKGTIMRMGDNQI
                     VDIDVIPTGSIGLNIALGVGGYPRGRVIEIYGPESSGKTTLAIHAIAEAQKAGGIAAI
                     IDAEHAFDRAYAEKLGVNTDELLISQPDNGEQALEIADQLIRSSAVDIVVIDSVAALT
                     PKAELEGDMGDSKMGLQARLMSQALRKLTANISKTNTTCIFINQLREKIGVMFGNPET
                     TTGGNALKFYASVRLDIRKIGQLKDGEEVIGHQTRVRVVKNKVAPPFRKAEFDIMFGE
                     GISKSGEILDLGVEYGIIKKSGSWFSYGDTKLAQGRDAAKTVIKDNPELAAELEAKIV
                     EAIKEK"
     gene            4152..4694
                     /locus_tag="PLZ54_07160"
     CDS             4152..4694
                     /locus_tag="PLZ54_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ66740.1"
                     /translation="MLENENIRLRAVEPEDLELLYRWENNPELWMVGNTRNPYSRYML
                     KRYILDSEKDIYENRQLRLMITIKSSNETIGTVDLFDFDIHNSRIALGLFIEQRFQRN
                     GFAREALHLVEEYVFEFLKINQLYVQIAEENIPSMRMFEEENFEKNGMLKNWIKTTND
                     FQNIIVFQQFKNVYSKTKMK"
     gene            complement(4797..5783)
                     /locus_tag="PLZ54_07165"
     CDS             complement(4797..5783)
                     /locus_tag="PLZ54_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HOJ66741.1"
                     /translation="MKEDNSLYPLKFQPILKEKIWGGTRLKKLFNKQTESDKIGESWE
                     LSGFEGDESIVSNGFLAGNNLTELIEIYMGDLVGDKIFEEFGLTFPLLFKLIDANDDL
                     SIQVHPDDETAAQRHNSLGKTEMWYVIDAEPGGELIVGFKNDCSREEYLKALEEGRVA
                     DLLKRVPVSKGDVIYIPAGLVHAIKKGVLVAEIQETSDLTYRIFDYKRTDEHGKEREL
                     HTELALDVINFSATKNPKVEYAPKVNAVTTLVSCDYFTTNLLIFNRNIIRNYSSLDSF
                     VVYMCLEGNFILECENHKILVEKGETVLIPASLDEVSLIPQGEVKLLEVYIQ"
     gene            complement(5806..6846)
                     /gene="galE"
                     /locus_tag="PLZ54_07170"
     CDS             complement(5806..6846)
                     /gene="galE"
                     /locus_tag="PLZ54_07170"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOJ66742.1"
                     /translation="MMSKVLVTGGTGYIGSHTVVELQQEGHEVIIVDNLFNSDIEVLN
                     GIEKITGIRPIFENIDCTDYVGMDRMFERHKNIDAIIHFAAYKAVGESVEKPLLYYRN
                     NILSLINLLQLMPVHKIKHLVFSSSCTVYGQPDILPVTENSPFKPALSPYGNTKQICE
                     EIIRDTIHSNSFYHSIVLRYFNPIGAHPSAEIGELPNGVPNNLLPFITQTAIGIRKQL
                     LVYGNDYNTPDGTCIRDYIDVVDLAKAHVSALNRMLNHQQKNNLEVFNLGTGRGLSVL
                     EIIKMFEKVNGVKVPYKIVGRREGDIEKVWADPSYANKELGWKAQRPLEETLRSAWEW
                     EKKLAERTKQNA"
     gene            complement(6853..7758)
                     /locus_tag="PLZ54_07175"
     CDS             complement(6853..7758)
                     /locus_tag="PLZ54_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HOJ66743.1"
                     /translation="MKPTLLILAAGMGSRYGGLKQLDGLGPNGETIMDYSIYDAIQAG
                     FGKVVFVIRKSFEKEFNELIVSKFKNLIDVELVFQELDKVPEGCTYATEREKPWGTNH
                     ALLMAKDVIHEPFGVINADDFYGRESYFILADFLRSVENKKNEYCMIGYRVGNTLSES
                     GPVSRGICVADENNFLKDVVERTHIVEKGGTIIYLDENGKEVAIHPNVPVSMNMWGFT
                     PDYFDYSWEAFKKFLAENGQNVKAEFYIPTVVNQLIHENKVTCKILDTPSKWFGVTYS
                     NDRPQTVLKINELIRKGVYPEKLFK"
BASE COUNT         2504 a         1590 c         1454 g         2415 t
ORIGIN      
        1 cagacatgtg ggttccatca gacctttaaa aaataaatta tattctgatg aaaaacaaaa
       61 ttcttttttc ctttttgtgt acaagtctgt tcttttcttg ccagccatct ttacattatg
      121 ctcccgatgg tgaaaacaat tttaacttaa attctctttt aacattggaa attggggaag
      181 ataccattta tttacaggat tttatactga atcctacaga aatcgactcc ataacttcat
      241 cttcctcctg gttacaatgg aaattatcca acgacaaaaa attactttat gttaaggttg
      301 tgccggaaat ggaaaatttt gtagatttaa aaatttggtt gaacggaacg ccttattcca
      361 tcccctgccg aaaaaccgac aaaacagatt ttgttttcac ttatcgacca cagggaaaag
      421 catatcgaaa agttcaaatt gctggacaaa tgaacgactg ggttcccaac tttacgcccg
      481 atttgcaact taacgacagc agctattatc aggtaacttt gcatttaagt ccgggaactt
      541 atctttatca gatggtgttg gatggagaat ggaattatga tgtcaacaat ccgcttaaaa
      601 tcgacaatgg aaggggaaaa ttcaattcca tattgaaagt tgaaggccag cagtcgctgc
      661 ttcccgaact ttacactgat gcgtattccg attccaaaat aacactttta tcggtaaaca
      721 aggtaaacaa tatttttgtt tactggcaaa attaccgttt gcccgacatg tttgtgaaga
      781 aacaggaaaa ccgcattatt ttcgatattc cttccgaagc tcattctctg gaacgttcgt
      841 ttattcgtgt ttgggtttcc aatgattttg gcgtcagcaa cgatgttttc attccgcttc
      901 acaagggaaa agtactgacg gactcaaaag aaattacgcg ttcggataaa gcatcccaaa
      961 ttatttattt tctgatggtc gatcggttta aaaacggcaa taaggataac gatcatcccc
     1021 ttcgtcgagc tgatgtacat cctaaagtcg atttttgggg tggcgatttg gccgggattc
     1081 gacaaaaaat tgatgaccgg tattttaccg atttgggagt aaatactttg tggatttctc
     1141 cggtgaatca aaatccgaca actccttatg gattttatgc accggtacct acaaaatttt
     1201 cagggtatca cggttattgg ccgatttctt cttccaaagt cgattttcgg tttggtacaa
     1261 acaacgaact gaaaaatctt gtggtccacg ctcattccaa caatatcaat attttactgg
     1321 attatgttgc ccatcatgta cacgaagaac atccacttta caaggaacat ccggagtttt
     1381 ttacttcatt gtatcttccg gacggaacat tgaatacgga aagatgggat gaccagcggt
     1441 tgactacgtg gtttgataca tttatgccga ctttgaacac caccgatccg aaagtgatcg
     1501 atttaatgac tgattcagca ctgttttggt taaaagaatt tcaactcgac ggctttcgac
     1561 acgatgcctg caagcatatt tcaaatgact tctggagaga actcactcta aaaataaaac
     1621 gtcaaaatca tggaaaaaat ttatatcaaa ttggcgaaac atatggaagt ccacagctta
     1681 ttggcagttt tttaggcacc ggcatgttgg atggtcaatt cgattttaat gtttttgatg
     1741 aagcttccgt tgcttttgcc ggcattggcg gaaattctct aagtcgggta aacgatgtgt
     1801 tgcaaaacag ttttaaggtg tatggacatc acaacttaat gggctatatt tcaggcaatc
     1861 acgatcgtcc ccgatttatg gcttatgctt cgggcgattt gaaatacgga gaagacacca
     1921 aactggccgg atggaaaagg gaaataggaa tttcagattc tacggcttac gataaaatgt
     1981 ttttattgca tacttttaac ttaactattc ccggtattcc cgttatttat tatggcgacg
     2041 aaattggcat gacgggcgcc aatgatcccg attgcaggag aatgatgcgt tttgaccatt
     2101 ggaacaagcg agagcaaaat ttatggaaaa aagtttctac tttgactcat ttaagacttc
     2161 aacatccggt actgatttat ggcgattttg tttcgcttca ggctaataac gatagctggg
     2221 tatatgcccg aaaatatttt gataaggtgg ccataatatt tatcaataat gcgcccaaat
     2281 cccagcattt tgaaatcaat cttccgagca tcatgacaaa cacaatttat caggctgttt
     2341 tccaacatgc ttttacagag gttaacggaa ccatcaacat agacttgccg gcctacagtg
     2401 ccgaaatatt actcaattag aaaaatacaa aaaaatgata caaatcccaa gttcagaatt
     2461 gcagcaagcc cttgaaaaag acaataaaga atttcttcgg acgattgcca atacctattt
     2521 gacagctatt ggtggtaatt tgaatacttc caacatgcaa ttgctcaatg gctggcaaca
     2581 tgttttgctt tcgtggtatt ttttttcgac cgaaatggat gaaggcggtt ttgttcaact
     2641 gattcaaaac ggttacggag ggtatatttt tgacaatcct tttgccaaag cattacgatt
     2701 gatgggagca cgtgagctgt cgaaactgat ttacagggca aaaaaaattt atgacgagaa
     2761 caaaaccacg ctggaaagag atacttccga agaagaattt aatgcaatgt atgttgattt
     2821 tgagcaattt gatgaattgg aagaagaata tttcgacatg gaagaagaac aactggaaat
     2881 aatttcagca tatgtaaaag gacatctttc cgattttact gaaatacttg ccaatcaggt
     2941 caatttttaa aattctatgt ttaacactaa aaataaagta ctgattttaa aaatattaaa
     3001 catcttccag tcaagattta tgttcactca catttgtaga tccattcatt ttcatgtatt
     3061 tttgtatcag tgaatttata ttaaagaagt tcagaaaaat aatcaattat taaaaactta
     3121 attatggccg aaaaaacaac cgaagaacaa aagaaaccgt cctccgaaaa aataaaagcc
     3181 ttgcagctgg caatggaaaa aatagaaaaa gattacggaa aagggaccat tatgcgtatg
     3241 ggcgacaacc aaatagttga catcgatgtg attccaaccg gctccatagg tttaaacatt
     3301 gctttaggcg ttggcggcta tcccagaggc agagtgattg aaatttacgg acccgaatcg
     3361 tccggaaaaa ccacattggc cattcatgcc attgctgaag ctcagaaagc cggcggcatt
     3421 gctgccatta ttgatgcaga acatgctttc gatcgtgctt atgccgaaaa gttgggtgta
     3481 aataccgacg aattgctgat ttctcagccc gacaatggcg aacaggcgtt ggaaattgcc
     3541 gatcaactga ttcgttcgtc agccgttgac attgtcgtta tcgattccgt tgccgcctta
     3601 actccaaaag ccgaactgga aggcgacatg ggcgattcaa aaatgggact tcaagcccga
     3661 ttaatgtcgc aggcacttcg caaacttact gccaacatca gcaaaaccaa cacaacctgt
     3721 atttttatca atcaacttcg ggaaaaaatt ggtgtcatgt ttggcaatcc tgaaactact
     3781 accggaggaa acgccctgaa attctatgct tccgttcgtt tggatattcg aaaaatcggg
     3841 caactgaaag atggagaaga agttatagga catcaaacgc gtgtgagagt agtgaaaaat
     3901 aaagttgcac ctcctttccg taaagcagaa tttgatatta tgtttggcga aggaatttcc
     3961 aaaagcggtg aaatcctcga tttgggggta gaatacggaa ttatcaaaaa aagtggttcg
     4021 tggttcagct atggcgatac caaacttgct cagggaagag atgccgcaaa aaccgttatt
     4081 aaggacaatc cagaattggc tgccgaactc gaagcaaaaa ttgttgaagc tatcaaagaa
     4141 aaataaaatt gttgctggaa aacgaaaaca tacggctacg cgctgtcgaa cccgaagatt
     4201 tggaacttct gtatcgatgg gaaaacaatc ccgaattgtg gatggtgggc aatacccgaa
     4261 atccctattc acgttatatg ctgaaacggt acatactgga ttcggaaaaa gacatatacg
     4321 aaaacaggca actgaggttg atgataacta tcaaaagcag caatgaaacc attggaacgg
     4381 ttgacttgtt tgacttcgac attcacaata gccgcatagc attgggactt tttatcgagc
     4441 aaagatttca aagaaacggt tttgccagag aagcacttca tctcgttgaa gaatatgtat
     4501 ttgaatttct gaaaattaac caactttacg tacagattgc tgaagaaaat atcccaagca
     4561 tgcgcatgtt tgaagaagaa aattttgaaa aaaacggcat gttgaaaaat tggataaaaa
     4621 ccacgaacga ttttcaaaat attatcgttt tccaacaatt caaaaatgtt tacagcaaaa
     4681 caaaaatgaa gtgatgtgat agatttgttt tgacagaata aaacaggaaa aagggaatgg
     4741 cacttcaact ctcgtttgca gccattccct ttttaatatt aagtttgaaa taatccttac
     4801 tgaatatata cttccagcaa ttttacctct ccttgcggaa tcaatgaaac ttcgtccaaa
     4861 ctggcaggaa ttaaaactgt ttcgcccttt tccaccaaga ttttatggtt ttcgcattct
     4921 aaaatgaaat ttccttccaa acacatataa actacaaagg aatccagcga actgtaattg
     4981 cggataatat tacgattaaa tattagcaga ttggtggtaa aataatcaca cgaaaccagt
     5041 gttgttaccg cattaacttt aggtgcgtat tcaacttttg gattttttgt ggcggaaaaa
     5101 tttatgacat ccaaagccaa ttctgtatgc agttcacgct cttttccatg ttcatcggtt
     5161 cgtttgtagt caaaaatacg ataagtaaga tcacttgtct cctgaatttc tgcaaccaat
     5221 acgccttttt ttatggcgtg taccaaacct gccggaatat aaatcacatc acccttgctg
     5281 accggaactc ttttcagtaa atcggccaca cgtccttctt ccaaagcttt caaatattct
     5341 tcgcgtgagc aatcgttttt aaatccaact atcaattctc ctcccggctc agcatctatt
     5401 acataccaca tttcggtttt tcccaacgaa ttgtgccgct gagctgccgt ctcatcgtcg
     5461 ggatgcacct gaatggacaa atcgtcattg gcatcaataa gtttaaaaag caagggaaaa
     5521 gtcaacccaa attcttcaaa aatcttatcc ccaaccaagt ctcccatata tatttcaatc
     5581 aattccgtca aattattccc ggcaaggaaa ccattcgata caattgattc atcaccttca
     5641 aaaccggaaa gttcccaact ttcaccaatc ttgtcggatt cggtttgctt gttgaaaagt
     5701 ttttttagcc gcgttcctcc ccaaattttt tcttttaaaa tgggttgaaa ttttaaggga
     5761 tataagctat tgtcttcttt cattttaatt gaatgaaatt ttttatcaag cattttgttt
     5821 ggtacgttca gccagttttt tctcccattc ccatgcactg cgaagcgttt cttccaaagg
     5881 tcgctgagct ttccaaccca attccttatt tgcataactc ggatcggccc aaactttttc
     5941 tatatcacct tcccgtcttc ccacaatttt ataaggtacc ttgaccccat taactttctc
     6001 aaacattttg ataatttcca gcaccgaaag tcctctgccc gttccaagat tgaaaacttc
     6061 cagattgttt ttctgctgat gattcaacat gcggttgaga gccgaaacat gtgcttttgc
     6121 caaatcaacg acatcgatgt agtcgcgaat acatgttcca tcaggcgtat tataatcgtt
     6181 tccataaacc agcaactgtt tcctgattcc gatggctgtt tgagtaataa acggaagcaa
     6241 attattgggc acgccattgg gcaattctcc aatttcggcc gaaggatgcg ctccaatcgg
     6301 attgaaatat cgaagaacaa tgctatgata aaatgaattg gaatgaatgg tatcgcgaat
     6361 aatttcttca catatttgtt tggtattgcc ataaggagaa agtgccggct taaaaggtga
     6421 attttccgtt accggcaaaa tatccggctg tccataaacg gtgcacgaag acgaaaaaac
     6481 caaatgcttg attttatgta ccggcatcag ttgcaataaa ttgatcagag aaagaatatt
     6541 attcctgtaa tacagcaaag gcttttcaac cgactcgcct acagctttat acgctgcaaa
     6601 atgaattatc gcgtctatat tcttatgtct ttcaaacatg cgatccatgc caacataatc
     6661 cgtacagtcg atattttcga atatgggacg aataccggta attttttcga ttccgtttaa
     6721 aacctcaata tcggaattaa aaagattatc cacaataata acttcatgtc cttcctgttg
     6781 caattcaacc acagtatgcg aaccaatata tccggttccg ccggtaacca aaactttact
     6841 catgatgtct gtttatttga ataatttttc tggatatact cctttacgga ttaattcatt
     6901 gatttttaaa actgtctgcg gtctgtcatt ggaatatgtt acgccaaacc acttggaagg
     6961 cgtatccaaa attttgcagg taactttatt ttcgtgtatc aattgattga caaccgtcgg
     7021 gatataaaat tcggctttaa cattttgacc attttcagct aaaaatttct tgaaagcttc
     7081 ccaagaatag tcgaaataat cgggagtaaa accccacata ttcattgata cgggtacatt
     7141 gggatgaatg gcaacttctt ttccgttttc atccaaataa attattgtac ctcccttttc
     7201 cacaatatgc gttctttcca ccacgtcctt taaaaaattg ttttcatcag ccacacatat
     7261 tcctctgctc accggtccac tttccgaaag cgtgttacct actcgataac caatcatgca
     7321 atattcattc tttttatttt ctacgctgcg caggaaatcg gccaaaataa aatacgactc
     7381 acgaccataa aaatcatcgg cgttgattac gccaaaaggt tcgtgaataa cgtcctttgc
     7441 catcagcagt gcatgatttg ttccccaagg tttttcgcgt tcggtggcat aagtacatcc
     7501 ttctggcact ttgtcgagtt cctgaaatac caactcaaca tcaattaaat ttttgaattt
     7561 actgacaatc aattcattaa attccttttc aaagcttttt cgaatcacga acactacctt
     7621 tccaaatcct gcctgtatcg catcataaat ggaataatcc ataatggttt ctccattggg
     7681 gcctagccca tccaattgct tcaatccgcc ataacggctg cccattccgg ctgctaaaat
     7741 taataaagtt ggtttcatat tttattttct gttgtttatt tcaaagattt aatatagttt
     7801 acagataatc aatgttttca gaaaaaattt attaggaaaa attcgagttt gcgctaaaag
     7861 ttttaggaaa tgcaaattta ctattattgt ttcaattaag aaaactgtaa actacttcat
     7921 aaacttaact ttgacaaaag tcgaaatttt ataatttcgt taa
//
LOCUS       DAOSCH010000093         7918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5932_length_7918_cov_5.934122, whole genome shotgun sequence.
ACCESSION   DAOSCH010000093 DAOSCH010000000
VERSION     DAOSCH010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5932_length_7918_cov_5.934122"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="PLZ54_07180"
     CDS             complement(<1..219)
                     /locus_tag="PLZ54_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66744.1"
                     /translation="MSENIINLHKNVDTNPTPMFKILSINPGSTSTKFAVYEDEKILF
                     LHTILHSTEELKSFEKIIDQLDFRKNLIL"
     gene            463..930
                     /gene="rimP"
                     /locus_tag="PLZ54_07185"
     CDS             463..930
                     /gene="rimP"
                     /locus_tag="PLZ54_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016271128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOJ66745.1"
                     /translation="MIEQTTILQIVENFVSGTDFYIVEVKVSPENQILVVFDSFEGIS
                     IDDCEALNNFIESKLDREQEDYELEVSSPGLTEPFKVLKQYEKNKGNEVEIVTKKGEK
                     FSGILTEVNFDNCTLQIEKLIKPEGAKRKIKVTEKVPFRYEDIKTTKYILRFK"
     gene            934..2190
                     /gene="nusA"
                     /locus_tag="PLZ54_07190"
     CDS             934..2190
                     /gene="nusA"
                     /locus_tag="PLZ54_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007756462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOJ66746.1"
                     /translation="MSKKETVNLIDTFSEFKELKNIDRSTLISVMEESFRNIIAKNYG
                     TDKNFDVIINPDKGDFEIYRNRTVVADDEVSDPNLEISLSEAQKIDEDYQIGEEVTDT
                     VDISDFGRRAILTLRQTLQSKILELQKDNVYTKYSKMVGQIVTAELYQIWKKEMLLMD
                     EEGNELYLPKSEQIPTDFYRKGDMVKAVVAMVENKNNNPRVILSRISPVFLERLFENE
                     IPEIHDGLITIKKIARIPGERAKVAVESYDDRIDPVGACVGMKGSRIHGIVRELRNEN
                     IDVINYTNNISLFISRALSPAKISSIRINEAEKKAEIYLKPEEVSQAIGKGGLNIKLA
                     SMLTGYTLDVFRELDETETEDIYLDEFNDEIDQWVIDTLKSIGCDTAKNVLAIPREDL
                     IRRTDLEEETIDEVLDILRSEFEDEN"
     gene            2255..5308
                     /gene="infB"
                     /locus_tag="PLZ54_07195"
     CDS             2255..5308
                     /gene="infB"
                     /locus_tag="PLZ54_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015532151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOJ66747.1"
                     /translation="MPIRLSKACKDLNVGMSTAVEFLAKKGHKLSADPNLKLEDELYL
                     LLAKEFNKDMALKIESERISKERQEKEKAETVGLEEYVHPKTAPKPAEIIPTVIPEEQ
                     KPHIKSVGHIDLEETGKKVKTKEKEKTEIVDSIPVEPIKEPVVAEKEKIEPDATFTHE
                     VKAVVTEEPVKPEKPEEIIQEQKSQPEEFEVTEKILPDSTEIKAEKIEEKIETQVITS
                     PEISKHRTTQESESSNLEEVNVNEAEIEISELTEEVFYTEIPVLDTNITVVGSIDLDA
                     LNQRVRPKPKSKEQRKREKEEKIQLEAEAKKIAQKGKKVIKLPGEAEDAEDTKDHHKK
                     KRERFTKQKVDISKVSGNANPEELERPKKLNLSPKGTKDKFKKSIKPAISEEDIQKQI
                     KETLARLATSNKKTKGSKYKKDKREIINARQQQLAEMEQSNEKVLKLTEFVTVNELAN
                     MMDVPVNDVISTCMNLGLIVSINQRLDAETINILADEFGFTTEYVSHDVIEALAENEP
                     DDEENLQPRPPIVTVMGHVDHGKTSLLDYIRKTNVIAGEAGGITQHIGAYNVVLDNGQ
                     RITFLDTPGHEAFTAMRARGAKVTDLAIIIIAADDNVMPQTVEAINHASAANVPIVFA
                     INKVDKPDANPEKIKEQLANMNYLVEDWGGKYQSQEISAKKGIGVPELLEKVLLEAEL
                     LDLKANPNRRAIGSIIESSLDKGRGYIATVLVQNGTLHQGDVVLAGTHFGKVKAMFNE
                     RNQRISEARPSEPVLILGLNGAPQAGDNFVVMESEHEARELALKREQLQREQTIRTKK
                     HFTLDELGRRIALGDFKELNVIVKGDVDGSVEAISDSLLKLSTEKIQVNIIHKAVGAI
                     SDSDVLLAAASNAIIVGFQVRPTPSARKMAEKEEIDIRLYSIIYDAIEEIKSAMEGML
                     SPEIKEEITATLEVRDVFKITKVGTVAGCIVKEGKIKRTNKVRLIRDGIVIFTGEIES
                     LKRFKEDVKEVAVNYECGVSIHNFNDIHVGDLIESFEEMEVKSEL"
     gene            complement(5577..6215)
                     /locus_tag="PLZ54_07200"
     CDS             complement(5577..6215)
                     /locus_tag="PLZ54_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ66748.1"
                     /translation="MNIKTFIFNPFRENTYIVSDVTGEAIIIDAGCLTNREKQAVKNY
                     IENNKLVLKHLLNTHLHIDHQFGNRYIYDNYHISPEACKEDEFLIETMHEAAKFFGID
                     LHEEPPAIGSYIIENQEIKFGNSILKALHVPGHSPGSMAFYSEQNGLVFTGDVLFYGT
                     IGATHFEKGDHDLLIRSIWEKLMVLPDETLVYSGHGQPTTIGNEKNFNPYLV"
     gene            complement(6254..6876)
                     /gene="rsmG"
                     /locus_tag="PLZ54_07205"
                     /pseudo
     CDS             complement(6254..6876)
                     /gene="rsmG"
                     /locus_tag="PLZ54_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
     gene            7233..>7918
                     /locus_tag="PLZ54_07210"
     CDS             7233..>7918
                     /locus_tag="PLZ54_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOJ66749.1"
                     /translation="MNHIPKLSHILVLILVVQLSSCITARKINYLQQPNASIPAYKDT
                     FSFNDYRLKKGDRVFIKIYSWDDKTNALLNGSSNIEQLVMSGSSSGNELYTYLIQPSG
                     KVNLPMVGEIYLEGQTVREAKETLEKFIQPVFDISSVDVRIAGRFFSVIGGGATGRYP
                     LNREKINIFEALAMAGDIDTYGDRSKIRIIRETNSGTQIKVFDVRSVDIINSEFYYVE
                     PNDVIYIQDM"
BASE COUNT         2785 a         1291 c         1569 g         2273 t
ORIGIN      
        1 aagaattaaa ttttttctga aatcgagctg atcaattatt ttttcaaacg attttaattc
       61 ttccgttgaa tgtaaaatgg tatgaagaaa taaaattttt tcgtcctcat agacggcaaa
      121 ttttgtagaa gtagaaccgg gattgattga aaggatttta aacatgggtg taggatttgt
      181 gtcaacattt ttatgcaaat ttatgatatt ttctgacata ttctaaatat ttgatttctt
      241 taaaggaaaa atctttgttt taagtaatgt ggttgcaata ccttaaataa ttcataatca
      301 gtcataaagt tataattggt tgttttattg taaaaagaga aaatgtttat ttgataaaac
      361 cttggatttt atataattat tttgtaattt tgcaacgtga aaagaaattg tgagagggga
      421 caaaattgtc cccttgtttt ttagataaat gtgtactttc cgatgattga acagacaacc
      481 atattgcaaa tagtagaaaa ttttgtttca ggcacagatt tttacattgt cgaagttaaa
      541 gtgagccctg aaaatcagat tcttgtcgtt tttgattcat ttgaaggtat ttccattgac
      601 gattgcgaag ctttgaacaa tttcatcgaa tccaaactgg acagagaaca ggaagattat
      661 gaattggaag tcagctctcc cggactgaca gaacccttta aagtactgaa acagtacgaa
      721 aaaaacaaag gtaatgaagt ggaaattgtt acaaaaaaag gagaaaaatt ttccggaata
      781 ctcacagagg taaattttga taattgtacg cttcaaattg aaaaattaat caaacccgaa
      841 ggtgcaaaaa ggaaaatcaa agttacagaa aaagttcctt ttcgatatga agatattaaa
      901 actacaaaat atattttaag atttaaataa gccatgagca aaaaagaaac tgttaattta
      961 attgatactt tttcagaatt taaagagctt aaaaacattg atcgaagtac tttaatcagt
     1021 gtaatggaag aatcgttccg caatattata gcaaaaaatt atggaaccga taagaatttt
     1081 gacgtcatca tcaaccccga taagggagat tttgaaatat atcgaaaccg cactgtagtg
     1141 gcagatgatg aggtaagcga tccaaattta gaaatatcgc tcagtgaagc ccaaaaaatt
     1201 gatgaagatt atcaaatagg agaagaagtt accgataccg ttgacatttc ggatttcggt
     1261 cgacgtgcca tcttgacttt gcggcaaacg ctgcaatcaa agattctgga gttgcaaaag
     1321 gataacgttt ataccaaata cagcaaaatg gttggtcaaa ttgttacggc cgaactatat
     1381 cagatttgga aaaaagaaat gcttctcatg gatgaagaag gaaatgagct ttatttgcca
     1441 aaatcagaac agattccgac ggatttttat cgaaaaggcg acatggtgaa agctgtagtg
     1501 gctatggtgg aaaataaaaa caacaatccg cgagtcattc tttcccgtat ttcgccggta
     1561 tttttggaac gactttttga aaatgaaatt cctgaaatac atgatggact gataaccata
     1621 aaaaaaatag ccagaattcc cggcgaacgc gctaaagttg ctgttgaatc ctatgacgac
     1681 cgcattgacc ctgttggagc ttgcgtaggt atgaagggtt caagaattca cggaattgtg
     1741 cgtgaattgc gaaatgaaaa tatagatgtc atcaattata cgaataacat cagtttgttc
     1801 atttccagag cattgagtcc ggccaaaatt tcatctatcc gcatcaatga agctgaaaag
     1861 aaagccgaaa tttatctgaa acccgaagaa gtttctcaag ccatcggaaa aggagggctc
     1921 aatattaagc ttgccagcat gcttaccggt tatacactcg atgttttccg cgaattggat
     1981 gaaaccgaaa cagaagatat ttacttggat gaattcaatg acgaaatcga tcagtgggtt
     2041 attgatacct taaaatctat cggttgcgat acggctaaaa atgtattggc cattccacgt
     2101 gaagatttga ttcgtcgcac cgatttggaa gaagaaacta ttgatgaagt gctggacatt
     2161 ttacgttcgg aatttgaaga cgaaaattga gaattttttc aataaatgaa caaaagttca
     2221 aagcttttaa agttaataat tgtaaaataa atatatgcct ataagattaa gtaaagcatg
     2281 caaggatttg aatgtcggta tgagtacggc cgtcgaattt cttgcaaaaa aaggtcataa
     2341 attgtcagcc gatccgaatt taaaattgga agatgagctg tatcttttac tcgcaaaaga
     2401 atttaataag gatatggcgt taaaaattga atcggaacgg atcagcaaag aacggcagga
     2461 aaaagaaaaa gccgaaactg tcggattgga agagtatgtt catcccaaaa cggcaccaaa
     2521 acctgccgaa ataataccta ctgtcatccc tgaagaacaa aagcctcata tcaaatctgt
     2581 gggacacatt gacttggaag aaacagggaa aaaagttaaa acgaaagaaa aagaaaaaac
     2641 ggaaattgtt gactcgattc ctgttgaacc aataaaggaa cctgttgttg ccgaaaaaga
     2701 aaaaattgaa cccgatgcta ctttcactca tgaagtaaaa gcggttgtta ccgaagaacc
     2761 agtaaaaccg gaaaaacctg aagaaataat tcaggaacaa aaatctcaac ctgaggaatt
     2821 tgaagttaca gagaaaattt tgcccgattc aacagaaatt aaggccgaga aaatagaaga
     2881 aaaaattgaa actcaagtaa taacttctcc cgaaatttcc aagcatcgga caactcagga
     2941 atcagaatct tccaatttgg aagaagttaa tgttaatgaa gcagaaattg aaatttcgga
     3001 actaacggaa gaagtttttt acaccgaaat acctgttttg gacaccaata ttactgtggt
     3061 gggaagcatc gaccttgatg cactcaacca gagggttaga ccaaaaccaa aatcgaagga
     3121 acagagaaag agggaaaaag aagaaaaaat tcagttagaa gctgaagcaa aaaaaattgc
     3181 tcaaaagggt aaaaaagtta ttaaacttcc cggtgaagca gaagatgcgg aagatacaaa
     3241 agatcatcac aaaaagaaac gcgaacgttt tacaaagcaa aaagttgata tttctaaagt
     3301 ttccggcaat gccaatccgg aggaattgga acgtccaaaa aaattaaatc tttctcccaa
     3361 aggaaccaaa gataaattta aaaaatccat caaacctgcc atttcggaag aagacataca
     3421 gaaacaaatc aaggaaaccc ttgctcgttt ggcaacttcc aataaaaaga caaagggttc
     3481 aaaatacaaa aaagacaaaa gagaaattat caatgcccgt cagcaacagc tggcagaaat
     3541 ggagcagagc aacgaaaaag tactgaaact gacggaattc gtaactgtga atgaactggc
     3601 taacatgatg gatgttccgg tcaacgatgt gatttctacc tgtatgaatc ttggtttgat
     3661 cgtttccatc aatcaaaggt tggatgctga aactattaat attttagccg atgaatttgg
     3721 cttcactacc gaatatgtca gccatgatgt gatagaagca cttgctgaaa acgaacccga
     3781 cgatgaagaa aatcttcaac ctcgtccacc tattgtaacg gtaatgggac atgtcgatca
     3841 tggtaaaaca tctttgctgg actacatccg caaaacaaat gtgatagccg gagaagccgg
     3901 aggaattacc cagcacattg gcgcttacaa tgtggtgttg gataatgggc agcgaattac
     3961 ttttctcgac acaccgggtc atgaagcgtt tacggccatg cgtgccagag gtgcaaaagt
     4021 taccgacctg gccatcatta ttattgctgc cgacgataac gtgatgcctc agactgtgga
     4081 agccataaat catgcttcgg cagccaatgt ccccattgta tttgccatca acaaagtgga
     4141 taagcccgat gccaatcccg aaaaaataaa agaacaattg gccaatatga attatttggt
     4201 ggaagattgg ggcggtaaat atcaatcgca ggaaatttca gcaaaaaaag gaatcggagt
     4261 gcccgaattg cttgagaagg tattgctcga agccgaattg ctcgatttga aagccaatcc
     4321 caacagaaga gcaataggtt ctattattga atcatcgttg gataaaggta gaggatatat
     4381 tgctacggtt ttggtacaaa atggaacgct ccatcaaggc gacgtagttt tggcaggaac
     4441 acatttcgga aaagtaaaag ccatgtttaa tgaaagaaat caaagaatct ctgaagcacg
     4501 accttcggaa cctgttttga ttttaggatt gaacggagca cctcaggccg gagataattt
     4561 tgtggtaatg gaatcggaac atgaagcgcg tgaattggca ttgaaacgtg aacagttgca
     4621 aagagaacaa acaattcgta ctaaaaaaca ttttacactg gacgaattgg gaagaagaat
     4681 tgcattgggc gattttaagg aattgaatgt cattgtaaaa ggtgatgttg acgggtcagt
     4741 tgaagctatt tccgactcgc tgttaaaact ttctaccgaa aaaattcagg taaatattat
     4801 tcataaggct gtgggtgcca tttcggattc cgatgtgttg ctggcagcag cttccaatgc
     4861 catcattgtg ggttttcagg tacgtcctac tccttctgcc cgaaaaatgg cagaaaagga
     4921 agaaatcgat attcgactct attccatcat atatgatgcc attgaagaaa ttaaatcggc
     4981 aatggaaggc atgctctcgc ctgaaattaa ggaagagatt acggctactc ttgaagtcag
     5041 agatgtgttt aaaattacaa aagtgggaac tgtagccggt tgtatagtga aagaaggaaa
     5101 aataaaacga accaacaaag ttcggttgat tcgcgatggt attgttattt ttaccggcga
     5161 aatcgaatcg ttaaaaagat ttaaagagga tgtaaaagaa gttgctgtca actatgaatg
     5221 cggtgtaagt attcacaatt tcaacgatat tcatgtagga gatttgatag aaagttttga
     5281 agaaatggaa gtgaaatcgg aactgtaagc cggcaatatt tctctttaat gctgtcgaac
     5341 actcagacaa gaatcatgaa aattttgaaa aagtcgccat gagttagaga ccgaaattaa
     5401 taagataccg caggtaagcc gatgaaatta taggacaaac aaaaaattcg aaacgaagat
     5461 agtttcaaaa atagcagtct tttcctgcaa ttgtcataaa atcaattgat gaaaaatctg
     5521 cattgaactg aaaaattttc cggaagattt gattttttta ttggaacaaa ctcttattaa
     5581 accaaatacg gattaaaatt cttttcgttt ccaatggtag tgggttgtcc gtgtccgctg
     5641 tagaccaatg tttcatcggg tagaaccatc agcttttccc aaatggaacg gattaataag
     5701 tcgtgatcgc ctttttcaaa atgggtggct ccaattgtgc cgtagaacaa gacatctccg
     5761 gtaaatacca aaccattttg ttctgaataa aaagccatgc ttccgggtga atggcccgga
     5821 acatgtaatg ctttcaaaat tgagtttcca aattttattt cctgattttc aataatataa
     5881 gagccaattg caggaggttc ttcatgcaag tcgataccaa aaaattttgc tgcttcatgc
     5941 atggtttcga ttaaaaattc atcttccttg catgcttcag gcgaaatgtg atagttgtcg
     6001 taaatataac gatttccaaa ttggtggtcg atatgcaaat gggtattgag taaatgtttt
     6061 aaaacaagct tattattttc aatataattc ttgacagctt gtttttcgcg gtttgtcaga
     6121 caacccgcat caataatgat ggcttcacca gttacatccg aaacaatata ggtgttttct
     6181 ctgaaaggat tgaaaataaa ggtttttatg ttcattttta tattctgtaa aggttttgcc
     6241 tgattaattt ttttcacaca acaggcaaat aaacaatttt tttgtcttta aaatattctt
     6301 ctttaaaaaa atcggaaata ctggctactt caattatctt tttgaaaggc ttaatttctt
     6361 gttccaaact accacccttt aaagaaataa ttccattggg taacgcattg atggaggttt
     6421 tccgaatatt tttttggtca ttttcagtaa atcttctagt ggcatgactg cacgactgac
     6481 aataaaatcg tattttccct tttcttcttc tgctcgtaaa tatttgaatt cggaattttt
     6541 caactcaatg gcctcagcaa ccgacattgc aactttaatt ttttttccta tggaatctat
     6601 cattacaaat ttaacttcgg gaaaaagtat agcaagcgga ataccgggaa atcctcctcc
     6661 ggtacccaca tccaaaatac ttgagttcgg cttgaaacgg atatattgag caatagcaag
     6721 agaatgaaga acatgatgtt cgtacaggtt ttgaatatct tttctcgaaa tcacatttat
     6781 tttttcattc caatccttat aaaggggata cagcgcttca aactgagctt tctgatgaga
     6841 tgacaagtcg ggaaaatatt tattgatcaa ttccataaac tcattttttg gcatacaaag
     6901 ttaagcgttt ttacaaaagt ataaaatttc atttgatcga ttcttaatat tcaaggcaaa
     6961 aaaccaattg aaaaaataaa taagctcatc tgacaatata ttaacaaaaa ggcttcattg
     7021 aaaggatcag gatatcaaaa tatttagaaa aatcttaacc gaatacctcg agataaattg
     7081 aattttaatt ggctttaaaa aagagttttt cgaacatttt ttaatttcga acttgccggt
     7141 tgactctact aatttctaat ttttttgtaa tgttatattt cttttgtaat tttgattaac
     7201 tttgttgagt gttttaaaaa gtagatttta gaatgaatca tattccgaag ttatcccata
     7261 tacttgttct gattttggtt gtacaacttt cttcgtgtat tactgcccgc aaaattaatt
     7321 acctgcagca gcccaatgct tccattcctg cgtataagga tactttttca ttcaacgatt
     7381 acagactgaa aaagggggac agggttttta taaaaatcta ttcatgggat gataaaacaa
     7441 atgcattgct aaatggtagc agcaatatag aacagcttgt catgtcgggt tcttcatccg
     7501 gcaacgaact gtacacttat ctgattcagc cctccggaaa agtaaatttg ccaatggtag
     7561 gagaaatata tttggaaggt caaactgtaa gagaagccaa agaaacgctc gaaaaattta
     7621 tacaaccggt ttttgacatc agcagcgtcg atgttagaat tgctggaaga tttttcagtg
     7681 ttattggtgg aggtgcaacc ggcaggtatc ctttaaatcg ggaaaaaata aatatttttg
     7741 aagcgctggc aatggccggc gacattgata cttatggcga tagaagtaaa ataaggatta
     7801 tcagagaaac aaacagtgga actcagataa aggtttttga tgtgcgaagt gtggatatta
     7861 tcaattccga attttattac gttgagccca atgatgtgat ttacattcag gacatgaa
//
LOCUS       DAOSCH010000094         7856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_5983_length_7856_cov_5.705038, whole genome shotgun sequence.
ACCESSION   DAOSCH010000094 DAOSCH010000000
VERSION     DAOSCH010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7856
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5983_length_7856_cov_5.705038"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..205)
                     /locus_tag="PLZ54_07215"
     CDS             complement(50..205)
                     /locus_tag="PLZ54_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66750.1"
                     /translation="MARVLRVTANLKEAISKADVLMNRNLIEGSNERVTEQWIGNARN
                     LIVTQME"
     gene            complement(319..1338)
                     /gene="pheS"
                     /locus_tag="PLZ54_07220"
     CDS             complement(319..1338)
                     /gene="pheS"
                     /locus_tag="PLZ54_07220"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOJ66751.1"
                     /translation="MKDRILQLQEEISHLTAQTLEEVEALRIKYLSKKGEISQLFNDF
                     RNVSNEEKREIGQLLNQLKNNAQDKINQLKEKLEANRGNVEKIDLTRTAEPIQIGTRH
                     PLSIVRNEIATIFSGIGFTIAEGPEIEDDWHVFEALNFPADHPARDMQDTFFIEKNPD
                     ILLRTHTSSVQTRVMTQQKPPIRMIFPGRVYRNEAISYRAHCFFHQVEGLYIDKNVSF
                     ADLKQALIYFAKEMFGMDTQIRLRPSYFPFTEPSAEMDISCNLCGGKGCPFCKYTGWV
                     EILGCGMVDPNVLENCGIDSKIYSGYAFGMGVERITNLKYQVKDLRMFSENDIRFLRQ
                     FESAY"
     gene            1550..2230
                     /locus_tag="PLZ54_07225"
     CDS             1550..2230
                     /locus_tag="PLZ54_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOJ66752.1"
                     /translation="MTIQKILDFLSVLSKNNNREWFAQNKSWYDEVRSGYLDMSNSLI
                     MKIAEFDPEIKHVDIKDCIFRIYRDIRFSPDKSPYKTHFGAYIAAGGGRKSQRGGYYF
                     HLQPESSFVSVGIWSPEPNVLKALRKSVYENIDEFNDILSQPNFKRFFGGFFEDGKLK
                     KVPAGFPGDFPQADLLKLKHYATSSPLDGSILTDENPVPYIAEILKAGYPLNRFLNFT
                     LDELFLNT"
     gene            2235..2681
                     /locus_tag="PLZ54_07230"
     CDS             2235..2681
                     /locus_tag="PLZ54_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HOJ66753.1"
                     /translation="MILDTLKNAELYESAHPLFKKAFDFLKTTDLKTLPLGKIELEGS
                     DLVVSVVDISGKTVQDARMEIHRKFIDIQLPLDGEEIMGWIPAEKLTKVLQPYDAEKD
                     IAFFADEAENLVKVRAMEFAVFFPSDGHQPGIGFGNHKKIIVKVRV"
     gene            2753..4753
                     /locus_tag="PLZ54_07235"
     CDS             2753..4753
                     /locus_tag="PLZ54_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HOJ66754.1"
                     /translation="MEILPIVKIDPWLEPYAGSIYGRYEYFLQTEKKLTGGKQSLSDF
                     ATGYLYFGLHRVADGWVFREWAPNATAIFLIGDFNNWQRHPDYQLKKLQNGIWEIHLP
                     EYAMGHTQLYKLLVEWEGGSGERIPAWAQRVVQDTQTKIFSAQVWNPFQPYEFKNRHF
                     KPQRDPLLIYECHIGMSSQEEKIASYDEFRRNVLPRIAALGYNAIQIMAIQEHPYYGS
                     FGYHVSSFFAPSSRFGTPEELKQLIDEAHGMGIAVIMDIVHSHAVRNEVEGLGRFDGT
                     PYQYFHGGPRREHPAWDSLCFDYGKPEVLHFLLSNCKYWLDVFHFDGFRFDGVTSMIY
                     LSHGLGEDFTSYDCYYNLNQDGDAICYLTLANKLVHQVNPNAISIAEEVSGMPGLAAK
                     IDDGGIGFDYRMAMNIPDFWIKIIKEEKDEDWKVGRIFWEMTNHRAEEMTISYAESHD
                     QALVGDKTIIFRLIDADMYWHMRRGDNTLTVDRGVALHKMIRLVTASTMNGGYLNFMG
                     NEFGHPEWIDFPREGNGWSYKYARRQWDLVDNKNYLYYCLNEFDRAMIMLIRSVPRFN
                     KLPIDQLWIKEDDQVLVFQRGKLVFVFNFNPSKSFTDYGILAEKGAYRTVLNSDNSLF
                     GGYDLIDESIVHVTLPEPTDPGGKEWLKLYLPARTAFVLRKD"
     gene            complement(4851..5246)
                     /locus_tag="PLZ54_07240"
     CDS             complement(4851..5246)
                     /locus_tag="PLZ54_07240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOJ66755.1"
                     /translation="MATIFSKIAAGEIPSYKIAENDLYFAFLDIHPLTKGHTLVIPKK
                     EEDYIFRLDDENYQGLMLFSKKIALAIEKAIPCKRIGMAVIGLEVPHAHVHLIPIQQE
                     NDMNFRNPKLELSSEEMDKIAASIRKYLE"
     gene            complement(5273..5746)
                     /gene="greA"
                     /locus_tag="PLZ54_07245"
     CDS             complement(5273..5746)
                     /gene="greA"
                     /locus_tag="PLZ54_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOJ66756.1"
                     /translation="MATVTYMTAEGYKKLVDELHQMENVQRPAISRQIAEARDKGDLS
                     ENAEYDAAKEAQGMLEMKIAILKDTIASARLIDESKIDTSEVQILTKVTIKNTKTNQV
                     MTYTIVSESEANIREGKLSVTTPIAKGLLGKKVGEKTSISVPNGVQEFEILDISL"
     gene            complement(5889..6251)
                     /locus_tag="PLZ54_07250"
     CDS             complement(5889..6251)
                     /locus_tag="PLZ54_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66757.1"
                     /translation="MKKKKKSDTPKPEEKKHYEQATWQELKPVVERREFEKVVKAPTS
                     KPVEEYMENTYATYDTVGNDISELRIKKPVKVSIENNHHLIEEDRYEESGTIPLELDS
                     PEAAKKAFLYSVIFERKY"
     gene            6682..7797
                     /locus_tag="PLZ54_07255"
     CDS             6682..7797
                     /locus_tag="PLZ54_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOJ66758.1"
                     /translation="MKYGFFVVVFSIFAFFFGYVGLRGWQSLALYPSARPTYIVSFLL
                     LFICSMAGMIFGDDMPASIAKVLTFTGYSFLLIFAYLFIVFLLVDVVRIANHFFHFAQ
                     SNMVSFRFWSSIVGLSVIIIAMIIGNYKFNHPQVVQLELQSNKPFQNKKIRIVAASDI
                     HLGTYIDKNYLKKYVQFINDQQPDIILLAGDICDRSLKPLIHQKMHEELHQLKAPLGV
                     FAILGNHEYYSQNLQNTVLFYKDAGIVVLADSISFVNNDFYLVGRKDKTDKNRQPLST
                     LLTTLEQDKPIILLDHQPLHLVEAAKNNVDLQISGHTHEGQVFPANLLVKSLFENGYG
                     YLKKGNTHIYVSSGLGLWGPQYRIGTKSEIVVIDFKF"
BASE COUNT         2259 a         1436 c         1575 g         2586 t
ORIGIN      
        1 caagacctct cgggataaga tattaaactt tcgctgcgtc ttgcctgatt tactccattt
       61 gagttacgat taaatttcgg gcgttcccaa tccattgctc ggttaccctc tcattagagc
      121 cttctatcag atttctgttc atcaaaacgt cagctttgct gatggcttct ttcagatttg
      181 cagtcacccg caacaccctt gccattcgct aatgtttcct atcaactcgg cacatacagg
      241 aacccgcatc ctgcaagctt aatgccatgt cagcaatatt cttaaaagcg ctctctgttt
      301 tttctgaata ctatcaaatc agtatgccga ttcgaattgt cgcaggaaac ggatgtcatt
      361 ttcagagaac atgcgcaaat ccttaacctg atattttaaa ttggtaattc gttcaactcc
      421 cattccaaag gcataaccgg aataaatttt tgaatcgata ccgcaatttt cgagtacatt
      481 gggatctacc attccgcaac ccaatatttc tacccagcct gtatatttgc aaaacggaca
      541 accttttccg ccacaaagat tgcacgaaat atccatttcg gcgcttggtt cggtaaaagg
      601 gaagtaggaa ggacgcagcc ggatttgtgt atccatgcca aacatttctt ttgcaaaata
      661 aatgagtgcc tgtttcaaat cggcaaacga cacattttta tcgatataca aaccttctac
      721 ctgatgaaag aaacaatgtg cgcgatacga gatagcttca tttctgtaaa cacgtcccgg
      781 aaaaatcatt ctgatgggcg gtttttgctg tgtcatcaca cgggtttgaa ccgaagaagt
      841 atgagtgcgc aagagaatat ctggattttt ttcgataaaa aatgtatcct gcatgtctct
      901 ggcgggatga tcggcaggaa agttcaaggc ttcaaaaaca tgccagtcgt cttcaatttc
      961 cggtccttcg gcaatggtga acccgatacc tgaaaaaatc gttgcaattt cgtttctgac
     1021 aatggaaaga ggatggcgtg ttcctatctg aatcggttca gcggtgcgcg tcaaatcaat
     1081 cttttccaca tttccccgat tggcttccaa tttttccttg agctggttga ttttatcctg
     1141 tgcattgttt ttcagttggt tgagcaattg cccgatttca cgtttctctt cattacttac
     1201 atttctgaaa tcgttgaaaa gctgtgaaat ttctcccttt ttgctcagat acttgattct
     1261 taaagcttca acttcttcga gcgtttgggc tgtaagatgg ctgatttctt cctgcaattg
     1321 tagaattcgg tctttcattt tttatattgg aatttgcaaa aaaattgaat aagtcctgag
     1381 aacagaaata acctgttatt atttttttaa attaactgca aaagtaatta ttttttacca
     1441 aaataggagg aatttatacc ggaaaaagga attacttcaa aaaaatgagg ataaatggca
     1501 aagattaaat aaattttaca ttatatttgt cgtgcttaaa atataaatta tgaccataca
     1561 aaaaatactc gattttctga gtgttttgag caaaaacaat aaccgtgagt ggtttgctca
     1621 aaataaaagt tggtatgatg aggtgcgtag cggttatttg gatatgagta attcactgat
     1681 aatgaaaata gccgaatttg atcccgaaat caagcatgtt gacataaaag attgcatttt
     1741 ccgaatttac agggatatac gtttttctcc cgataaatcg ccatacaaaa ctcattttgg
     1801 tgcttacatt gctgcaggcg gtggtagaaa aagtcagcgg ggtggttatt atttccattt
     1861 acaaccggaa agttcttttg tttcagtggg aatttggagc cctgagccca atgtgctgaa
     1921 agcgttacga aaaagtgtgt acgaaaatat cgacgaattt aacgatattc tgtctcaacc
     1981 gaatttcaaa cgtttttttg gcggtttttt cgaagatgga aaattaaaaa aagtaccggc
     2041 aggttttccc ggtgattttc cgcaggccga cttgctcaaa ttaaagcatt atgcaacttc
     2101 ctcaccattg gacggatcca ttttaacgga tgaaaatcct gttccgtaca ttgcagagat
     2161 tttgaaagct ggctatccgc tcaacaggtt tttaaatttt actttggacg aattattttt
     2221 aaacacataa agccatgata ctcgatacct taaaaaatgc cgaattatac gaatcggcac
     2281 atccactttt caaaaaagct ttcgattttc tgaaaacaac cgatttgaaa actttgcctc
     2341 tcggaaaaat cgaattggaa ggcagcgact tggttgtcag cgtggtggat atttccggga
     2401 aaacggttca ggatgcccgc atggaaatac atcggaagtt tatcgacatt cagcttccac
     2461 ttgatggcga agaaataatg gggtggattc ctgccgaaaa gttgacaaaa gttttacaac
     2521 cttacgatgc tgaaaaagat attgcttttt ttgccgatga ggcagaaaat ctcgttaaag
     2581 tgagagcgat ggaatttgct gttttttttc cttccgatgg tcatcaaccg ggaattggct
     2641 ttggaaacca caaaaaaatt atagttaaag ttcgtgttta ggagtacaat tttataccac
     2701 acccagaaaa tcggatttgt ttttggacaa aaccacatta agctaaaaaa ttatggaaat
     2761 tcttccaatt gttaagattg atccgtggct tgaaccttat gcgggttcta tttatggaag
     2821 atatgaatat tttcttcaga ctgaaaaaaa actgacgggc ggcaaacaga gtttgtccga
     2881 ttttgcaacc ggatatttat attttggact tcatagagtg gcggatggtt gggtttttcg
     2941 agaatgggca cccaatgcta cggccatttt tttaattggc gatttcaata attggcaacg
     3001 tcatcccgac tatcagttaa agaagttgca aaacggaatt tgggaaatac atttgcctga
     3061 atacgcaatg ggccatacac aactttacaa acttttggta gaatgggagg gcggaagtgg
     3121 cgaaagaatt ccggcttggg ctcagcgtgt ggtacaggat acgcaaacca aaattttcag
     3181 tgcgcaggtg tggaatcctt tccaacctta tgagtttaaa aatcgacatt tcaaaccaca
     3241 aagagacccg ctgttgattt acgaatgcca tatcggtatg tcgagccagg aagaaaaaat
     3301 tgcttcttat gacgaatttc gccggaatgt acttcctcgc attgctgcgc tgggttacaa
     3361 tgccattcag attatggcta ttcaggagca tccttattac ggttcgttcg gctatcatgt
     3421 ttccagcttt tttgctcctt catcgcgttt tggtacgccg gaagaactca aacaactgat
     3481 tgatgaagct catggaatgg gaattgccgt tatcatggat attgttcatt ctcatgcagt
     3541 gagaaatgaa gtggaaggat tgggtagatt tgacggaacg ccttatcagt actttcacgg
     3601 gggcccgcga cgtgaacatc ctgcttggga ttctttgtgt ttcgactacg gcaaacccga
     3661 agttttacat ttccttttgt ccaattgcaa atactggctg gatgtttttc atttcgacgg
     3721 tttccgtttc gatggcgtaa catcgatgat ttatctcagt catgggcttg gagaagattt
     3781 taccagttac gattgttatt ataatctgaa tcaggatggc gatgccattt gttatcttac
     3841 tctggccaac aaactggttc atcaagtaaa tcccaatgcc atctccattg cggaagaagt
     3901 gagtggaatg ccgggattgg ctgcaaaaat tgatgatggc ggaattggct tcgattaccg
     3961 aatggccatg aatattcccg atttctggat taaaattatc aaggaagaaa aagatgaaga
     4021 ctggaaagtt ggacggattt tttgggaaat gaccaatcat cgtgcagagg aaatgaccat
     4081 cagttatgcc gaatcgcacg atcaggcttt ggttggcgac aaaacgatta ttttcagatt
     4141 gatagatgcc gatatgtatt ggcacatgcg tcgtggcgac aacactttga ctgtcgatcg
     4201 cggagtggcg ctgcacaaaa tgattcgtct ggttacggct tcaaccatga atggtggtta
     4261 tctgaatttt atggggaacg agttcggaca tcctgaatgg atcgattttc cgcgcgaagg
     4321 caacggttgg tcatacaagt atgcccggcg tcagtgggat ttggtggaca acaagaatta
     4381 tctctattat tgtttgaacg aatttgacag agccatgatt atgctgattc gttccgtgcc
     4441 aagatttaat aaacttccca ttgaccagtt atggataaaa gaagacgatc aggtgctggt
     4501 ttttcaaagg ggaaaactgg tgttcgtatt taattttaat ccttcaaaat cgtttaccga
     4561 ctacggtatt ttggcggaaa aaggtgctta cagaactgtt ttgaattccg acaattcctt
     4621 atttggaggt tatgatctga ttgatgaaag tattgtgcat gttactttgc ccgagccaac
     4681 agatcccggc ggaaaagaat ggctgaaatt atatctcccc gcgcgaacag cttttgtttt
     4741 gagaaaagat tgaaaagttt tttgatttgg agctactcca tctgaatacg ataccccaaa
     4801 agcttcgagt aacttttggg gtgttgcatg taaagttgga acaaggaaac tcactccaaa
     4861 tattttcgga tagaagctgc aattttgtcc atttcttcgg atgacaattc caatttcggg
     4921 tttctgaaat tcatgtcgtt ctcctgttga atgggaataa gatgaacgtg agcatgaggc
     4981 acttccagcc caatgactgc cattcctatc cgtttgcagg gaatggcttt ttcgatggca
     5041 agggcaattt tttttgaaaa aagcatcaat ccctgatagt tttcatcgtc caagcgaaaa
     5101 atgtaatctt cttctttttt gggaatcacc aaagtatgtc cttttgttaa aggatgaatg
     5161 tcgaggaaag caaaataaag gtcgttttcc gcaattttat aactgggaat ttctccggct
     5221 gcaattttac tgaaaatggt tgccatgatt ttaaaagttt aactttccgt tattatagtg
     5281 aaatatcgag aatttcaaat tcctgtacgc cattgggtac tgaaatggaa gttttttcgc
     5341 ccactttttt acccagcaaa cctttggcta tcggagttgt aaccgacaat ttgccttctc
     5401 tgatattggc ctcactctct gaaacgatgg tgtaggtcat tacctgattg gttttggtat
     5461 tttttatggt tactttggtt aaaatctgca cttcactggt gtcgattttc gattcgtcaa
     5521 tcaaacgtgc cgatgcaatg gtgtccttga gaatggcaat tttcatttcg agcattccct
     5581 gagcttcttt tgctgcatca tattcagcat tttccgacaa atcgcctttg tcgcgagctt
     5641 cagctatttg cctcgagatg gccggacgct ggacgttttc catttgatga agttcatcca
     5701 ccagtttctt ataaccttca gcagtcatgt atgttactgt tgccatagtt ttattttttt
     5761 aatttgaata aaaatataaa aacaaaaaga attccgaatc atgtaaagat cggaactctc
     5821 tctgttatgt ttttcgatgt ttatttgact acaaatatag tattttttat aataacaaaa
     5881 aaataatttt aatattttct ttcaaaaatt actgagtaaa gaaaagcttt ttttgcagct
     5941 tcgggtgaat ccaattccag aggaattgtg ccggattctt cgtagcgatc ctcctctatg
     6001 agatgatggt tattttcaat ggaaactttg acgggttttt tgattcggag ttctgaaata
     6061 tcgttgccga cggtatcgta tgttgcataa gtattttcca tgtactcttc aaccggcttg
     6121 ctggtaggag cttttaccac cttttcaaat tcccttcttt caactacggg cttaagctct
     6181 tgccaagtcg cttgctcgta atgttttttt tcttccggtt tcggtgtatc gctttttttc
     6241 ttcttcttca tattgctcag tagcaccacg actacgaaaa taagaaatat gtaattgcca
     6301 aagtcttcca taatttattg ttgatgttat tgaaaagcaa atttacagta aatctttgaa
     6361 tattttaaaa aaaatagaga aaaaatcgat ttatttaaaa catcaatttc atcagaatat
     6421 aatttatttt tttaaaggtc tgatggagcg cacatgtctg aatattatat tcattcgtgt
     6481 ttgtgtatgt aatacctgtg ggtttcataa ttaattatca aaaaactatt ttctcggtat
     6541 agggtcatga acaattttcg taaaaatacg tttttatgag caattgataa ttcaaaattt
     6601 aaagtgtcct atttaatttt ttttgaacca aagccgctaa aagtaaacgc acagctttga
     6661 ttaattaaac caaaatatat tatgaagtac ggattttttg ttgttgtttt ttctattttt
     6721 gcgttttttt ttggatatgt cggtttacgt ggttggcaat cgctggcctt gtatccatca
     6781 gcacgaccta cctatatagt cagttttctt ttgttgttta tttgttcaat ggctggcatg
     6841 attttcggcg atgacatgcc ggcatcaatt gccaaagttt taacatttac tggatattcg
     6901 tttcttctga tttttgctta tttattcatt gtttttttgt tggttgatgt ggttcgaata
     6961 gccaatcatt tttttcattt tgctcaaagc aacatggttt ctttcagatt ctggagctca
     7021 attgttggtt tatcggtaat tataatagcc atgattatag ggaattacaa gtttaaccat
     7081 cctcaagtag tacaactcga attgcaaagt aataaacctt ttcaaaataa aaaaatcaga
     7141 attgtggctg ccagtgatat acatttgggc acttatatcg ataaaaatta tctaaaaaaa
     7201 tatgttcaat tcatcaatga tcagcaaccg gatataattt tgttggcagg cgatatttgt
     7261 gatcgctccc tcaaaccttt gattcatcaa aaaatgcacg aagaattgca ccaactgaaa
     7321 gctcctttgg gtgtctttgc cattctgggc aaccacgagt attattctca gaatttgcaa
     7381 aatacggttt tattttataa agatgccgga attgtagtac tcgccgatag catttcattt
     7441 gtcaacaatg atttttatct tgtaggacga aaggacaaaa ctgataaaaa caggcaaccc
     7501 ctttcaacat tgttgacaac gcttgaacaa gataagccga taattttact tgatcatcag
     7561 ccgttgcatt tagtagaagc ggcaaaaaac aatgttgatt tgcaaatttc gggacatact
     7621 cacgaaggac aggttttccc tgccaatttg cttgtaaaat ctttatttga aaacggctat
     7681 ggatatttga aaaaaggaaa cactcatatt tatgtttcgt ctggacttgg tttatggggg
     7741 ccgcaatatc gaattgggac aaaatcggaa attgtagtga tagattttaa attctgaaag
     7801 atatataatt tctaataatt aattgcacat aatattttca aagtctcatt cggctt
//
LOCUS       DAOSCH010000095         7782 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_6060_length_7782_cov_5.606186, whole genome shotgun sequence.
ACCESSION   DAOSCH010000095 DAOSCH010000000
VERSION     DAOSCH010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6060_length_7782_cov_5.606186"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(267..2822)
                     /locus_tag="PLZ54_07260"
     CDS             complement(267..2822)
                     /locus_tag="PLZ54_07260"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOJ66759.1"
                     /translation="MNSFIIKQLKNYAILLLFIQSFQALSAQKTEVPFSTDSNYLTVW
                     NGKKYIPIFIKGVNLGVAKPGSYPGELDVKPEQYRQWFEEIKDAGFNCIRLYTLHFPQ
                     FYDELLKYNTNHPQHPLFFFQGVWLNEEMEGYKNDLYQITDTFRAEIRENIDCVHGNK
                     LIAQRYGKAYGNYTADVSKWNIGYIVGREVYPEEILTTDSIHAGNNYFSGTHFEINNA
                     SPSEVWFTENLDLLVEYEKSTYNTERPVSISSWPTLDPLHHAYEPNRTEDTATVDLSK
                     INILDAPAGLFISYHAYPYYPDFVGAQPDYKLATDNFGSNSYLGYIRDLKKHYRKYPL
                     LIAEYGVPSSWGIAHYTSSGMNHGGFDEQQQGETDLRLLRNISSSQLAGGIQFAWIDE
                     WFKKTWITDPIDFGNRILWHNITAAEQNFGLKKFVKSDEWKIWKNFDAADDITTLSAK
                     QNYDFFEIQLNLKDKMDLLGECWIAIDTYDAGLGESMMPNGQTIPSRAEFLLKITQYA
                     ATLYVTEAYDLFGLWHKILTPKQKLRSVATDGATWNIVRWKNNSFYSDVQYIGELKLN
                     KSFQPETSKDAVTIYDDKISIRLPWTLLQFVDPSQKEVFHVDSLNPELRTRESDGIAV
                     SIFYKNKTYFSDTRFDWEKWNKVTDNDVEEKFKSSYWTMYENLTEFNTPAIAFPDNYE
                     LEGEESIFKIDEYKGVLKNDFDMDGWNISAVLTDAPANGFVEINVDGSFSYIPRKGFK
                     GTDVFKYAIFDGTSLSQNTTVTLHVKKEGKQNPDNKKFFSLFPNPASDIVSIKSEINI
                     VSVRIFNYSGKLMEEFLLNDKTGTLNIFHYPAGLYIAMIDVDGQLFTEKFIKK"
     gene            complement(2834..3274)
                     /locus_tag="PLZ54_07265"
     CDS             complement(2834..3274)
                     /locus_tag="PLZ54_07265"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOJ66760.1"
                     /translation="MKSAESSDKTNSDPVIPFKFDIEPIDSQHQNLFRIFQSILDNIQ
                     QKSGNTEQIIEELEEYTHYHFSTEEDLMKKFDYPEINEHIVQHEIFIQKILDFKISQL
                     FQSITLNEEIAGFLRKWFLNHILEYDAKYVDTFKKNLNSENEEK"
     gene            complement(3365..4816)
                     /locus_tag="PLZ54_07270"
     CDS             complement(3365..4816)
                     /locus_tag="PLZ54_07270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ66761.1"
                     /translation="MEFPIIIEIVNWLIFVYACALFISYLLIALLSTSELKKYHSRNR
                     YSDFLKMLSFQKLPSVSIIAPAYNEEKTIVDNINCLLSLKYKDFEIIIVNDGSKDQTL
                     NKIIQYFDLIKTDMAYESQIITKNIRGIYKSRNNAYNHLTIIDKENGGKADALNAGLN
                     YAHNELFLAVDVDSILEPDALLRMVKPFIEETKVKVIASGGVIRVANSCEIKNGRIIK
                     VNFPKNQWAKYQTIEYFRAFTLGRMAWSRINGLLIISGAFGMFDRQTALAAGGYDTST
                     VGEDLELVVRMRRYMHEVEKKKYKIAFIPDPLCWTEVPESLKMLSRQRNRWTRGAIET
                     IRKHMKMAFNPKYGRIGMISFPYWIFFEWLAPIIQFLGIGYVILLTIFGLLDVKVFLI
                     MTLFVLSFAVMYSFFSVLLEAIYYNKYKNVTYLGNILLLSVMEMFVYQPINMLFSLSG
                     NIDYFFKKKSRHIWGNMTRKGFSGNTDQNSKQN"
     gene            complement(4822..6033)
                     /locus_tag="PLZ54_07275"
     CDS             complement(4822..6033)
                     /locus_tag="PLZ54_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66762.1"
                     /translation="MRTISLYLKFLFSRLIFIIALCLAVFLFFFLKQKEFILFNYSIG
                     NPLYSIIEQSPSWLYVVQVLIVIFLLLIVAELISIVLVTTRRSSAEKKNQLLHERIRE
                     VIFNYLIQDNKTRDINDFRIKAQPYLKSNHAQRVFINQLRKILILTKGEVHETCISLY
                     DALHFDPLIKSHFHSPYLRNKLFALKTAGDFQLTKYNRYLVRYMHSKNEMLRSEALQA
                     YVKANSDNDLSFLSDYQEKISLWDFNIIINAAKDRTNINFSALLKSHNPLIKSLGLRL
                     ANLQKKEELKPIIFKLMNDADPMVKEEAYLAFMALAKEKNDYLTLMYQFSCMTKNLQN
                     KVINLLINNEDKTSSYNFFEWIIENKPLEMKIRAMSKLLTMDMNLVLKYKNHPDENIR
                     KALRQLMDFYL"
     gene            complement(6037..7290)
                     /locus_tag="PLZ54_07280"
     CDS             complement(6037..7290)
                     /locus_tag="PLZ54_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HOJ66763.1"
                     /translation="MKKKFQLIVFLIMLASTGFLQAQTNVDSIFAVAIEKAHGGAYAA
                     AIREAKTALSADTARGDICVFLANVYSWSQKNDSALLFIDKAKKLNYLNEEFYDSYLN
                     ILLRSGKYEELEAACEEAEKNNYGDTKNLLTKKLIAYAQTGAYEKGIGLFKDEKNKVY
                     LQEKTIDELYTYLLMKQRTNLLSVNYSLDMFDNNDPQHLFSLGNAMQFKNRNIGLNAN
                     YAHRFGKNDVQIELNDYSVIYKKNYLYFNYGYALTGDLFPRHRTGLEYYFAISKHWDG
                     SLGGRYMFYPNADNKNVVIFTGNVGHYFGNNWVSLRPFYVVQNNLQSLSVVFKYRLYG
                     SDNRNFWGLEAGVGNSPDDILTTSQSSFNELMSYRIKLEKNFKVNRISDVNIGLGYMY
                     EQINQNATKQFRNRYLVEIGYKYRF"
     gene            complement(7315..7704)
                     /locus_tag="PLZ54_07285"
     CDS             complement(7315..7704)
                     /locus_tag="PLZ54_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOJ66764.1"
                     /translation="MAYRINIISNEIVFLTLIRNFIKKIELDIQISETDSLNMLYKEL
                     KENSFDLIIVDGNFSENSAIDLLYIIRFKLKIFTPIWFFTVIQTPEYYNKALQVGANV
                     IIERPFDPIEIASQIHEFLHNKVSVKN"
BASE COUNT         2312 a         1371 c         1437 g         2662 t
ORIGIN      
        1 tttattacga aatttgtata accaaaaagt tgcatttgtg acttgaattc agcaagtctt
       61 tcaacaaaga acaaaataaa tctcattgat ctttttattg gtaatttttc tatcattttt
      121 ttatagtgtt tactaatctc gacaaattta ttcaacctta tttacaacta ttttattgat
      181 attgattttt tgaatttttt cagctcatta taaaacaaaa tatggaagaa agtttgttga
      241 aaagaacagg ctttcttcct tttttttcat tttttgatga atttttcggt aaagagttgt
      301 ccatccacat caatcatggc gatgtacagg ccggctggat aatggaaaat attcaaagtt
      361 cctgttttgt cgttcagcaa aaattcttcc attaattttc cggaataatt aaaaattcgt
      421 acggaaacaa tattgatttc cgattttatg gaaacaatat cggaagcagg attggggaag
      481 agcgaaaaaa actttttgtt gtcgggattt tgtttgcctt cttttttgac atgtaaagtt
      541 acggttgtgt tttgggatag agaagtaccg tcaaaaattg cgtatttgaa cacatctgta
      601 cctttaaatc ctttacgagg aatgtaagaa aacgaaccat caacgtttat ttcgacgaaa
      661 ccgttggcag gtgcatcggt taaaacagca gaaatgttcc aaccatccat gtcaaaatca
      721 ttttttagta cacctttgta ttcatcgatt ttaaaaatac tttcttctcc ctccagctca
      781 taattgtcgg gaaaagcaat ggccggagta ttgaattcgg tgagattttc atacatagtc
      841 cagtaggaag atttgaattt ttcctccaca tcgttatcgg taactttgtt ccatttttcc
      901 cagtcgaagc gcgtatcgct gaaataggtt ttatttttgt aaaaaatgct caccgcaata
      961 ccatccgatt ctcttgtacg tagctcagga tttaaactgt caacgtggaa aacttctttt
     1021 tgagacggat cgacaaattg caataaagtc cagggcaatc ggattgaaat tttgtcgtca
     1081 taaattgtaa cggcatcttt tgaagtttcc ggctgaaagg atttattcag ttttaattct
     1141 ccaatgtatt gaacatccga ataaaaagag ttgtttttcc agcggacaat gttccatgta
     1201 gcgccatctg ttgccacaga acgcaacttt tgttttggtg tgagtatttt gtgccacaat
     1261 ccgaataaat cataagcttc cgtaacatac aaggttgctg catattgagt gattttgagc
     1321 aaaaattcgg cacgtgaggg gatggtttgt ccgtttggca tcatggattc tcccaaacct
     1381 gcatcgtacg tatcgattgc tatccaacat tctcccaaca aatccatttt atctttcaga
     1441 ttcaactgaa tttcaaaaaa atcgtaattt tgttttgctg acaaagtcgt tatgtcatca
     1501 gcagcatcaa aatttttcca gattttccat tcgtcggatt ttacaaattt tttcaggcca
     1561 aagttttgtt cggcagcggt tatgttgtgc cataaaattc tgttgccaaa atcaatagga
     1621 tcggtaatcc atgttttttt gaaccattcg tcaatccacg caaattgtat tcctccggcc
     1681 agttgactgg atgaaatgtt tctgagaaga cgcaaatcgg tttcgccttg ctgctgttca
     1741 tcaaatccgc cgtgattcat acccgaagaa gtataatgag caatacccca actggaagga
     1801 actccgtatt cggcaataag caaaggatat tttcgatagt gtttttttaa atctctgata
     1861 tagcccaaat aactgttgct gccaaaatta tccgttgcaa gtttataatc gggctgagcc
     1921 cctacaaaat ctggataata gggataggca tgatagctga tgaaaagtcc ggccggagca
     1981 tccagtatat taattttcga caagtctaca gttgctgtgt cttctgttct atttggctca
     2041 taagcatgat gcaggggatc aagggtgggc caggaagaaa tgctgacagg tctttcggtg
     2101 ttgtatgtgc ttttttcata ttctaccaaa agatccagat tttccgtaaa ccaaacttct
     2161 gaaggtgaag cgttattaat ttcaaaatgg gtacctgaga agtaattgtt gcccgcgtga
     2221 atgctgtcgg tagtgagaat ttcttcagga tatacttccc gaccgacaat gtatccgatg
     2281 ttccattttg agacgtcggc agtatagttc ccataagctt ttccgtaacg ctgcgcaatc
     2341 agtttgtttc catgaacgca gtcaatgttt tccctgattt cggctctgaa ggtgtcggta
     2401 atttgataca aatcgttttt gtatccttcc atttcttcgt ttagccatac gccttgaaaa
     2461 aagaataaag gatgctgcgg atggttggta ttgtatttga gcaattcgtc ataaaattgt
     2521 ggaaaatgaa gtgtgtaaag gcgaatacaa ttgaatccgg catcttttat ttcttcaaac
     2581 cattggcgat attgttcggg cttcacatcc agctcgcccg gatacgaacc gggtttggca
     2641 acacccaaat tgactccttt gataaaaatg ggaatatatt tttttccgtt ccaaaccgtc
     2701 agatagttgc tgtctgtgga aaagggaact tctgtttttt gcgctgaaag agcttgaaaa
     2761 gattgaatga acaaaagtag gatagcgtaa tttttcagtt gttttattat gaaactattc
     2821 atgtaatatt ttttcatttt tcttcatttt cggagtttaa atttttttta aaggtatcca
     2881 cgtatttggc atcatattcc aatatgtggt tgagaaacca cttgcgtaga aagccggcaa
     2941 tttcttcatt taaagtaata ctttgaaata attgagaaat tttaaaatcg agaatttttt
     3001 gtataaaaat ttcatgttga acaatgtgct catttatttc cgggtagtcg aattttttca
     3061 tcagatcttc ttcggtactg aaatgataat gggtatattc ttccagttct tcgataattt
     3121 gttccgtatt tccagatttt tgttgaatgt tatccaagat gctttgaaat atacgaaaca
     3181 gattttggtg ttggctatca attggttcaa tatcaaactt aaatggaatt acagggtcgg
     3241 aatttgtttt gtccgaactt tctgcgcttt tcataacgat tgttttttta atgtgctttt
     3301 attttctttt tcatataaat tcaagaaatc taaaaaattc agatttttac gtaaaatact
     3361 tatcttaatt ctgctttgaa ttttgatcgg tatttcccga aaaacctttt ctggtcatgt
     3421 ttccccagat atgtctgctt tttttcttaa agaaataatc gatgtttccc gacaaggaaa
     3481 atagcatatt aatcggctga taaacaaaca tttccattac agacaagagt aaaatattgc
     3541 ccaaataggt aacattttta tatttgttgt aataaattgc ttctaaaagt acggaaaaaa
     3601 atgaatacat gacagcaaaa gaaagaacaa aaagtgtcat aatcaggaat acttttacat
     3661 ccaacagccc gaaaatagtc agcaaaatta cgtaaccaat gcccaaaaac tgaataatgg
     3721 gcgccaacca ttcgaaaaat atccaataag ggaagctgat cattccgatt cgtccatatt
     3781 ttggattaaa agccattttc atgtgttttc gtatggtttc gatggctccg cgtgtccatc
     3841 ggtttctttg tcgcgaaagc atttttaatg attcgggaac ttccgtccag cacaatggat
     3901 cagggataaa ggctattttg tatttctttt tttctacttc gtgcatgtaa cggcgcattc
     3961 tgacaaccag ttctaaatct tcgccaacag tagaagtatc gtaacctccc gcagccaatg
     4021 ctgtttggcg gtcgaacatg ccgaaagcgc cggaaattat gagcaatccg ttgattctgc
     4081 tccaagccat gcgccctaag gtaaatgccc ggaaatattc gatggtctga tattttgccc
     4141 attggttttt cgggaaattc actttaatta ttctgccatt ttttatttcg caggaattcg
     4201 ccacacgaat cacaccgccg gaagcaatta ccttgacttt ggtttcttca ataaaaggtt
     4261 ttaccattcg cagcaatgca tcgggttcga gaatggaatc aacatcaacg gccagaaaca
     4321 attcattgtg agcataattc aacccggcat tcagtgcatc ggcttttcca ccattttctt
     4381 tgtcaatgat ggtcaaatga ttgtaagcgt tgtttcgtga tttgtaaatt cccctgatat
     4441 tttttgttat aatttgtgat tcgtaagcca tgtcagtttt aatcaggtcg aaatattgaa
     4501 taattttatt gagtgtttgg tctttactgc cgtcgtttac aatgataatt tcaaaatcct
     4561 tgtatttcaa gcttaaaagg cagtttatat tatccacaat tgttttttct tcgttgtaag
     4621 ccggagctat aattgaaact gaaggcaatt tctgaaaact caacattttc aaaaaatctg
     4681 aatatctgtt tcttgaatga tattttttta gttctgatgt tgacaaaagc gctatcagaa
     4741 ggtaggagat aaaaagggca catgcataaa caaagatgag ccagttcaca atttcgataa
     4801 tgatgggaaa ttccataatt tttaaagata aaagtccatt aattgacgga gtgcttttct
     4861 gatattttcg tcagggtggt ttttgtattt caaaaccaaa ttcatgtcca ttgttagcag
     4921 ttttgacatt gccctgattt tcatttccag cggtttgttt tcgatgatcc attcaaaaaa
     4981 attgtacgaa cttgttttat cttcattatt gatcagaaga ttaataactt tattttgtaa
     5041 attttttgtc atgcaactga actgatacat taaagtcagg taatcgtttt tttctttggc
     5101 taatgccata aatgccagat aagcttcctc tttcaccatt gggtcggcat cattcatcag
     5161 tttaaaaata atcggtttaa gttcctcttt tttttgtagg ttggcaagtc ttaatcccaa
     5221 actctttatt aatgggttgt gagatttcaa aagagcggaa aaatttatat ttgttctgtc
     5281 ttttgctgca tttatgataa tgttaaagtc ccagagagaa attttttcct gataatccga
     5341 cagaaaactg aggtcgttat cggaattggc ttttacataa gcctgcagtg cttccgatcg
     5401 taacatctcg tttttactgt gcatatagcg gacaagatat cggttgtatt ttgtcagttg
     5461 aaaatcacct gccgttttca gagcaaataa tttgtttctg aggtatggag aatgaaaatg
     5521 actttttata agcggatcaa aatgcaacgc atcatacaaa gaaatacagg tttcgtgaac
     5581 ttcgcctttg gtgaggataa gaattttacg cagttgattg ataaaaactc tttgggcatg
     5641 attggatttc aagtagggtt gagcttttat acgaaaatca ttgatgtctc ttgttttgtt
     5701 atcctgaatc agatagttga aaatcacttc acgaatcctt tcatggagga gttggttctt
     5761 tttttcggca gaagatcttc gagttgtaac caaaacaatc gaaatcagtt cagcaacaat
     5821 cagcaacagg aagataacaa tcagcacctg aaccacataa agccatgaag gcgactgttc
     5881 gataatggaa tacaaaggat tgccaatcga atagttaaac aggataaatt ctttctgttt
     5941 cagaaagaaa aacaaaaata cggccagaca caaggctatg ataaaaatca accttgaaaa
     6001 aaggaatttc agatataagc tgattgttct catttttcag aatcggtatt tgtagccaat
     6061 ttccacaaga tagcggttgc ggaactgttt tgtggcattt tgattgattt gttcgtacat
     6121 ataacccaac ccgatattaa catctgaaat tctgttcacc ttaaagttct tttccaactt
     6181 gatacgatag gacattaatt cgttaaatga actttgcgaa gtggtcagta tatcgtcggg
     6241 agaattgcct acgccggctt ccaagcccca aaaattcctg ttgtcgcttc cgtacagacg
     6301 atatttgaat acgaccgaaa gcgattgcaa attgttttgt accacataaa aaggtcttag
     6361 cgaaacccaa ttatttccga aataatgtcc cacattgccg gtaaagataa caacattttt
     6421 gttgtcggcg tttggataaa acatgtatcg accgcccagt gagccatccc aatgcttgct
     6481 tatggcaaaa taatattcca gtcccgttcg gtgacgggga aacaaatcgc cagtaagagc
     6541 atatccataa ttgaaataaa gatagttttt tttgtaaata acggaataat cattcaattc
     6601 gatttgcaca tcattttttc cgaaacgatg tgcatagttg gcattaagtc cgatatttct
     6661 gtttttaaac tgcatggcat tgcccaaaga aaaaagatgt tgcggatcgt tgttgtcgaa
     6721 catgtcgagc gaataattta ccgaaagcaa attggtgcgc tgtttcatca gcagataagt
     6781 gtaaagttca tcaatggttt tttcttgcag ataaactttg tttttttcgt ctttaaacaa
     6841 accgattcct ttttcgtatg caccggtttg agcataggca atgagttttt ttgtcaacag
     6901 gtttttagta tcgccataat tgttcttttc tgcttcttcg caggcagctt ctaattcttc
     6961 atatttccct gaacgcagca aaatattgag gtaactgtca taaaattctt cattgagata
     7021 gttcaatttt tttgccttgt ctataaacag taaagcagaa tcgttttttt gcgaccacga
     7081 gtaaacgtta gccaaaaaaa cacaaatatc accccgagca gtatcagcag acaaagccgt
     7141 tttagcttcc cgaatggctg ctgcataagc tccaccatga gctttttcta tagcaacggc
     7201 gaatatggaa tcgacattgg tttgagcttg cagaaaacca gttgaggcaa gcatgatcag
     7261 aaaaacaatc agttgaaatt tttttttcat attgttggtt cgtttgtata actttcaatt
     7321 ttttacagag actttattgt gcagaaattc gtgaatttgt gaggcaattt ctatgggatc
     7381 gaatggcctt tcaataataa cattggcacc aacctgaagt gctttgttgt aatattcggg
     7441 cgtttgaata accgtaaaaa accagatagg agtaaaaatt ttcagcttga atcgaatgat
     7501 atacaacaag tcaatggcag agttttctga aaagttgcca tctacaataa ttaaatcgaa
     7561 gctattttct ttcagttctt tatacagcat gttgagcgaa tcggtttcac tgatttgaat
     7621 atccaattca atttttttga taaagtttct gattaatgtc aagaaaacga tttcgtttga
     7681 aattatattg attcggtatg ccatttaata attctttttt tgattctgaa ttttaaaaat
     7741 tgtgtttatt ctgttcgctg atattcttcg tttaaaattt gt
//
LOCUS       DAOSCH010000096         7662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_6170_length_7662_cov_4.059813, whole genome shotgun sequence.
ACCESSION   DAOSCH010000096 DAOSCH010000000
VERSION     DAOSCH010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6170_length_7662_cov_4.059813"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="PLZ54_07290"
     CDS             complement(<1..521)
                     /locus_tag="PLZ54_07290"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOJ66765.1"
                     /translation="MRLFIQSEILQPFEEITHFFTTRHGGVSGGEYASFNLSPYVGDE
                     EENVHRNFEILSRQLGIGTSDIIFPYQVHDCEIREIDRSFFGLSHEQRQHFLNGVDAL
                     FTQLPGVVISITTADCVPLLFYAPEKKVIAVAHAGWRGTCAGIAAKTIETLVGKFDLS
                     PEDIFVAAGPSISP"
     gene            990..1652
                     /locus_tag="PLZ54_07295"
     CDS             990..1652
                     /locus_tag="PLZ54_07295"
                     /inference="COORDINATES: protein motif:HMM:NF036560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HOJ66766.1"
                     /translation="MLIRFENSIQQQEIEYFRGAVIHLIPSSEILFHNHLQDGFRFSY
                     PLIQYKRIANKAAIMCFNEGTESIGSLLANHNHDIQIGDRIEKLEIAKVQAFDYQIQI
                     WEPMFRYHIRKWLPFNQKNFEDYNRLESIAEKCLFLENILTGNLLSFAKGLHIFFDKQ
                     VVCKILQLENSTLTTYKGIKMNQFDAVFSCNVSIPDFTGLGKGVSIGYGMVTKYKNNT
                     KN"
     gene            1657..4188
                     /gene="cas10"
                     /locus_tag="PLZ54_07300"
     CDS             1657..4188
                     /gene="cas10"
                     /locus_tag="PLZ54_07300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HOJ66767.1"
                     /translation="MDFEREQVYLAALLHDIGKFYQRADTGSVAGSKYLKEYSKIEST
                     FLPLSVNGKYSHKHCLWTAQFIEDFQEVFRKLVGADSANLTDKNNLMFLAAAHHLKNE
                     QLSPLGQIIKKADCLSSGMDRNSLEALEDDQDESKWNSFKTKRMVSIFDILNKNKEEL
                     KQQKNWHHLPIDALNLSKNYFPRSEFNENPDYEKLWENFLIEFKFIQANTYRAFSETL
                     LNLLFKYTSCISSSTIVFPDVSLYDHSKTTAALAVCLYDYQHGTAASKDPFLLIGGDM
                     SGIQMYIYQIVSKYAGKNLKGRSFYIQILSDAVVRFLLKKLHLFRANIIYDSGGSFYL
                     IAPNTTFVNQQLTDAILFIEQQMFEKHGIQLYMAIDAVTVSEDALMHKGEEHLGDIWN
                     KLFEKRNKKKNNKYLSLITNSYDKLFTPFMQGGLTKRDRISGEEFFPNEKPFYSPEIS
                     EEFNEENPIKEITGKQILMGKYLRETDFLVVSEQKIPYWDDKIFIAPIELGFYYYFLK
                     TAEIEKMKERLQASADKITLVTLNGKNGNCDFMGDIKIRGLNNIYELKFYGGNEFNGK
                     TFDKMCDSESLSRLGILRMDVDNLGNIFQTGILPEMSTLSRFSALSRSFDFYFSGYLN
                     MLQQEIAPDSSFIIYSGGDDLFIVGDWRATIELAKKIKADFKEFSCHNPSLHISGGIA
                     IVEPTFPIMKSAEESQKEEKAAKEHTCKEAVKNSISFMDMPLHWEKEFPTVELLKNTF
                     VEIIKKGSPKSLLGKIMQHTANADIRNHKVHRNKTYWMLTYDLSRMKERNKDHSLLND
                     LIDNCLKEVCGNVQTSLNGLSITTEYHPLELWAFAARWAELELRS"
     gene            4237..4704
                     /gene="csm2"
                     /locus_tag="PLZ54_07305"
     CDS             4237..4704
                     /gene="csm2"
                     /locus_tag="PLZ54_07305"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOJ66768.1"
                     /translation="MNNYQKNPQQGGNWPQKNSQNYDQPKETPEEFLNHHAIDSKWIT
                     QNTDKSMVDFAEEAGKYMAKQKLTKSKIRSIYGEIKRIQINGFDKEKASFYLLRPKMA
                     YALGRDSSNYGLKLFKLIFDKSSELVQNSNQYENFCNLFEAILAYHKAFGGKD"
     gene            4761..5396
                     /gene="csm3"
                     /locus_tag="PLZ54_07310"
     CDS             4761..5396
                     /gene="csm3"
                     /locus_tag="PLZ54_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009012138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOJ66769.1"
                     /translation="MSTIKLNKKINYTGTIVLKTGLHIGGTNAALNIGGPDNFVVRNP
                     LTNVPYIPGSSLKGKMRALIEISTGDVSSKGEASENPNGVAGKLFGVGANKGGHPSRL
                     IVRDAELIIDDPEVDFSRTDLLYAESKTEVTINRVTAEANPRTFERVPAGAKFNLNMV
                     LNIFEGENEDELKRTLEKAMQLLENDYLGGQGSRGYGQVKFENIEITESKY"
     gene            5408..6391
                     /gene="csm4"
                     /locus_tag="PLZ54_07315"
     CDS             5408..6391
                     /gene="csm4"
                     /locus_tag="PLZ54_07315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm4"
                     /protein_id="HOJ66770.1"
                     /translation="MSEYKIIKFTQFTPLHVGTGKEDYDSSAGMLQSDTLSAALAAIR
                     AQMGKTDDVLEFMQSFTISSAFPYWKELYFMPAMKGELQVAVKGKEEYEYRKWLKKIK
                     FIEIGLWNELVSGKSIVVEQQQLQNEFLIPKISFQKVIKNQVNQRVQVPRIDGTKNKL
                     FSFDWQFFDKEAGLYCLIDADEKVFAEVKELFKLLGETGIGTDKNVGGGKFVPECGAI
                     QIPEIPDANATLLLSLFLPEEAELSQLKLEDAVFELILRGGFLAGSTEEKFRHLRKKS
                     VYMFNAGSLFPTVQEIKGKIVDLSPAWNDEKMHPAYRSGKPFCINIKVPSL"
     gene            6388..7548
                     /gene="csm5"
                     /locus_tag="PLZ54_07320"
     CDS             6388..7548
                     /gene="csm5"
                     /locus_tag="PLZ54_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR01899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOJ66771.1"
                     /translation="MSNIQLTTLTPVHVGSGELLQYNADFVEDKIGSNSYIYVVEPAK
                     ILQLIGMENIDRWISIIENAGNTKEFVQKFSPIKSPEAFSSRKILNFAKKIQKGQILN
                     ETIHNGFGFPYIPGSSLKGAIRTAVLASLVGRVNDKEEKILSKDRKGKTIISDKKIQN
                     ELFGNDAKNDLFRFVEVGDAYFDKGSDAAFRMIHLNITNKNSLLDDSKSQIVETIDQE
                     KKSVFRLNINLNRYHFAKEKWDQAVSYSKNKVINSPGKLPECLQSVENLFQTINAHTL
                     KLVNDEIKFWKEIDQKKAGADNYLDVMKSIQGEIINCTKGKECILRLGHASGWRFITG
                     AWAENLNNFYSEVVPAARPNNDKYPDDVFPKTRRVEEEYQDLLGFVKLTLIH"
BASE COUNT         2633 a         1294 c         1473 g         2262 t
ORIGIN      
        1 ggcgaaatgg aaggccctgc cgccacaaaa atatcttccg gcgaaaggtc aaatttacca
       61 accagcgttt caatggtttt tgcggctatt ccggcacaag ttccccgcca accggcatga
      121 gccacagcaa tcactttttt ctcgggagca tagaacagca atggcacaca atcagcagta
      181 gtaatgctga taactactcc gggcaattga gtaaacagtg catcaacacc attgagaaaa
      241 tgttgccttt gctcgtgcga taatccgaaa aatgaacgat caatttcgcg gatttcgcaa
      301 tcgtgaacct gatagggaaa tattatgtcg gaagttccaa ttcccaattg ccgggacaat
      361 atttcaaaat ttcggtgcac attttcttct tcatcaccca catagggact aagattgaac
      421 gaagcgtatt caccaccgct taccccacca tgccgggttg tgaagaaatg tgttatttct
      481 tcgaaaggct gcagtatttc tgattgaata aacaaacgca ttgaaaatcg atttataata
      541 agtaaattat ctctcagaga agaaagaaaa atttgatttt acgccagaaa gacaatttaa
      601 ttttgctgaa taattatata aaatatgcga aaattcggta aattcattgc tcctattcat
      661 ctttatcact aatcaaaagt tcagacttta taaactatcc ctttggcaaa attataaaaa
      721 atattcattc ccttcacaaa atcccaagcc tgtaagaatg atggtgtgaa agttatgctt
      781 atcagaaaaa agtttctcat tatcaagctg acaaacaggt tttaattgtt gattaaaaaa
      841 acaaaaaatt aattgacttt aaacatgata attcttatta aattttataa atttgtacca
      901 attaaaattc tacaagtcaa ttttttctga attattcttt cagaattcaa ccttttttaa
      961 ttactaataa catgaaaaac actcgattaa tgttaattag gtttgaaaac agcattcagc
     1021 aacaagaaat tgaatatttt cgaggtgctg tgattcattt aatcccatcg tccgaaattc
     1081 tttttcacaa tcatcttcaa gatggctttc gtttttctta tccactgatt caatataaga
     1141 gaattgccaa caaagcagca atcatgtgtt ttaacgaagg cacggaatcc attggctctc
     1201 tgttggcaaa tcataatcac gatattcaaa ttggcgatcg tatcgaaaag cttgaaatag
     1261 caaaagtaca agctttcgat tatcaaattc agatatggga accaatgttt cggtatcaca
     1321 ttcgtaaatg gttgccgttc aatcaaaaaa attttgagga ttacaatcga ttggaaagta
     1381 tagccgaaaa atgtcttttt ctcgagaata ttttgacagg caatctttta tcttttgcaa
     1441 aaggtcttca tatttttttc gacaagcaag tcgtttgtaa aattttgcaa ttggagaatt
     1501 cgacacttac cacttacaaa ggaataaaga tgaaccagtt tgacgctgtg ttttcctgta
     1561 atgtttctat tcccgatttt actggtcttg gtaagggtgt tagtattggt tacggtatgg
     1621 ttacaaaata taaaaacaac acaaaaaatt aagtctatgg attttgaaag ggaacaagtt
     1681 tatctggctg ctttactgca cgatattggt aaattttacc agcgtgctga tacgggcagc
     1741 gttgctggca gcaaatacct gaaagagtat agtaagattg aaagtacttt cctaccgctt
     1801 tcagtgaatg ggaagtattc ccacaagcat tgtttgtgga cagctcagtt tattgaagat
     1861 tttcaagaag ttttcagaaa acttgtcggt gccgattccg ccaatctcac cgacaaaaat
     1921 aatttgatgt ttttggctgc tgctcatcat ttgaaaaatg aacagctttc accgttaggg
     1981 caaatcatta aaaaagccga ttgtctctcg tctggaatgg ataggaatag cctcgaagcg
     2041 ttagaagatg atcaggatga atcaaagtgg aattctttca aaacaaaacg aatggtttct
     2101 atttttgata ttttgaataa gaataaggaa gaattaaaac aacaaaaaaa ctggcatcat
     2161 ttacccattg acgcacttaa tctatcaaaa aattattttc caagaagcga atttaatgaa
     2221 aatcccgatt acgaaaaatt gtgggaaaat tttctgatag agttcaaatt tattcaggca
     2281 aatacctatc gtgcttttag cgaaacattg ttgaatctgt tgtttaaata cacttcttgc
     2341 atttcttcga gtacgattgt ctttccggat gtctcccttt acgaccattc aaaaaccact
     2401 gcagcattag cagtttgtct ttatgattat caacatggaa ccgccgcaag caaagatcct
     2461 tttctcctga tcggaggtga tatgagtggt attcaaatgt atatttatca aattgtttcg
     2521 aaatatgctg gcaaaaatct aaaagggaga tctttttaca ttcaaatcct ttccgatgca
     2581 gtggtacgtt ttttattgaa aaagcttcat ctctttcgtg ctaacatcat ttatgattca
     2641 ggtggaagtt tttatctgat tgctccaaat actacatttg taaaccaaca actcacagat
     2701 gccattctgt ttattgaaca gcaaatgttc gaaaaacatg gtattcaact ttatatggca
     2761 attgatgcag taacggtttc tgaagatgct ttaatgcata aaggagaaga gcatttgggc
     2821 gatatatgga ataaactttt cgagaaacgt aacaaaaaga aaaataataa atacttatcg
     2881 ctcatcacaa atagttacga caaattgttt actcccttta tgcaaggtgg attaaccaag
     2941 cgcgaccgta tttcggggga agaatttttt cccaatgaaa aacctttcta ttcgccagag
     3001 ataagtgagg aatttaatga agaaaaccca attaaggaaa ttaccggtaa acagatatta
     3061 atgggaaaat acttgcggga aacagatttt ttggtcgttt ccgaacagaa aattccttac
     3121 tgggatgata aaatttttat tgctccgatt gaacttggat tttattatta ttttttgaag
     3181 acagctgaaa tcgaaaaaat gaaggaaaga ttgcaagctt ctgccgataa aattaccctc
     3241 gttactttga acggtaaaaa tggcaattgc gattttatgg gagatataaa aattcgagga
     3301 ttaaataata tttatgagct taaattttat ggaggtaatg aatttaacgg aaaaacattt
     3361 gacaaaatgt gtgatagtga atcattgtct cgtctcggaa ttttgcgaat ggatgtggac
     3421 aatctgggca atatatttca aaccggtatt ttacccgaaa tgtccacctt gtcgcgtttc
     3481 tccgctttaa gccgttcctt tgatttttac ttttcgggtt atctcaatat gcttcagcaa
     3541 gagattgctc ccgattcgtc atttatcatt tacagtggtg gagacgactt gtttattgtt
     3601 ggtgactggc gggcaactat tgaattagca aagaaaataa aagccgattt taaagaattt
     3661 tcctgccaca atccgtcctt acatatttcg ggaggaattg ccattgtaga gccaaccttc
     3721 ccgatcatga aatcagctga agaaagtcag aaggaagaaa aagcagccaa agaacatacg
     3781 tgcaaagagg cagtgaaaaa ttctatttct tttatggata tgcccttaca ttgggaaaaa
     3841 gagtttccta ctgttgaatt gttgaaaaat acatttgtgg aaattataaa aaagggttct
     3901 cctaaatcgt tgttggggaa aatcatgcag catacagcta atgccgatat cagaaatcat
     3961 aaagtgcata ggaacaagac atattggatg cttacatacg atttgagtcg aatgaaagaa
     4021 cgaaacaagg atcattcgtt attgaacgat ttgattgaca actgcctgaa agaagtgtgt
     4081 ggcaatgtgc aaacttcact caatggactt tctatcacta ctgaatatca tccgttggag
     4141 ctctgggctt ttgctgcccg ttgggcggaa ttggaactgc gttcctgaat taaaaagtaa
     4201 tttacattaa tataaaactc ttaaaatcaa taaattatga acaattatca aaagaatcct
     4261 caacagggag gaaactggcc acaaaagaat agtcaaaact atgaccagcc aaaagagacg
     4321 ccggaagaat ttttaaatca tcatgctatc gattcgaaat ggataaccca aaatacggat
     4381 aagtcgatgg tggattttgc agaagaagca ggaaaatata tggccaaaca aaaattaacc
     4441 aaatcgaaaa tcagaagtat ttacggggaa atcaaacgca ttcagattaa tggtttcgat
     4501 aaagaaaaag catcttttta tttacttcgt ccaaaaatgg cctacgctct tggtcgtgac
     4561 agtagtaact acggactgaa actttttaaa ttgatttttg acaaatcttc agagttggtt
     4621 caaaatagca atcaatacga gaatttttgc aatctgtttg aagccattct ggcttaccac
     4681 aaagcttttg gaggtaaaga ttaaaagtag attagaattt attaaaaaac actttcaatt
     4741 tcattaaaaa tctaaaaatc atgtcaacaa taaaattaaa caaaaaaatc aattataccg
     4801 gtactatcgt tttgaaaacc ggattgcata ttggtgggac aaatgcagct ttgaatatag
     4861 gcggacccga taactttgta gtaagaaatc cattaaccaa cgttccctat attccgggaa
     4921 gctcgctgaa gggaaaaatg agagctttga ttgaaatttc aacaggagat gtaagttcca
     4981 aaggggaagc gtcggaaaat cccaatggag ttgccggaaa gctgtttggt gttggtgcaa
     5041 ataaaggtgg tcatccttcc cgtttgatcg tgagagatgc agaactgata attgatgacc
     5101 ctgaagtcga tttcagcaga acggatttac tctatgccga aagcaaaacg gaagtaacta
     5161 tcaatcgagt aaccgctgaa gccaatcctc gtacttttga acgagtaccg gcaggggcca
     5221 aattcaatct gaacatggta ctcaatattt ttgaaggtga aaacgaagat gaattgaaaa
     5281 gaactttgga gaaagccatg caattactcg aaaatgacta tttgggcgga caagggtcac
     5341 ggggctatgg gcaggttaaa tttgaaaata ttgaaattac agaatcgaag tactaaattt
     5401 cagtaatatg tcggaatata aaataataaa attcacccaa tttacgcctt tgcatgtagg
     5461 aacggggaaa gaagattacg attcctcagc aggaatgctt caaagcgaca ctttgtcggc
     5521 tgcccttgct gcaattcggg ctcaaatggg taaaacggac gatgtgctgg aatttatgca
     5581 gtcgtttacc atcagttctg cttttcctta ttggaaagag ctctatttca tgccagcaat
     5641 gaaaggagaa cttcaagtag cagtaaaagg aaaagaagaa tatgaatatc gaaaatggct
     5701 gaaaaaaatt aaatttatcg aaataggctt gtggaacgaa ttggtttccg gaaaaagcat
     5761 tgttgttgag cagcaacagt tgcaaaacga atttttgatt ccaaaaattt cttttcaaaa
     5821 agtcatcaaa aatcaagtga atcaaagggt acaagttcca cgaatagatg gaacgaagaa
     5881 caaacttttt tcttttgatt ggcaattttt tgacaaagaa gccggacttt actgcttgat
     5941 agatgctgat gagaaggtgt ttgccgaagt gaaagaactt ttcaaactgt tgggcgaaac
     6001 aggaataggt accgataaaa acgtaggcgg aggaaaattt gttccggaat gcggagcaat
     6061 tcaaattcct gaaattccgg atgcgaatgc aacacttttg ttgtcgcttt ttttaccgga
     6121 agaagccgaa ctctctcagt tgaaactgga agatgccgtc tttgaattga ttttacgggg
     6181 aggatttttg gctggtagta ccgaagaaaa atttcgtcac ttgaggaaaa aatcggttta
     6241 tatgtttaat gcaggttcat tattcccaac cgttcaagaa ataaaaggaa aaattgtgga
     6301 tttatcgcca gcatggaatg atgaaaaaat gcatcctgct tatcgaagcg gaaaaccatt
     6361 ttgtatcaac attaaagtac catcattatg agtaatattc aattaacgac tttgacacct
     6421 gtccacgtag gttcgggaga gttattgcaa tacaatgccg attttgtgga agataagatt
     6481 ggatcaaatt cttatattta tgtagtagaa cctgctaaaa ttctccaatt gattggcatg
     6541 gaaaatatag accgctggat ttcgataatt gaaaatgccg gaaacaccaa agaatttgtt
     6601 cagaaatttt cacccatcaa aagtccggaa gcattttctt ctcgaaaaat tttgaatttt
     6661 gccaaaaaaa ttcagaaagg acaaattttg aatgaaacca ttcacaacgg ttttggattt
     6721 ccctatattc cgggcagttc tttgaaaggc gccatacgta cagcagtgct tgcttcgctg
     6781 gtaggaaggg taaatgataa agaggaaaaa atactttcga aggacagaaa aggaaaaaca
     6841 attatttccg ataaaaaaat tcaaaatgaa ttgtttggca atgatgccaa aaacgatttg
     6901 ttccgatttg tggaagtggg tgatgcttat ttcgataaag gttcggatgc tgcctttcgg
     6961 atgattcatt tgaatattac caacaaaaac tcactgctgg acgacagtaa atctcaaatc
     7021 gttgaaacca ttgatcagga aaaaaaatcc gtttttcgtt taaatatcaa tttgaataga
     7081 tatcattttg caaaagaaaa atgggatcag gctgtcagtt actctaaaaa caaagtaata
     7141 aattcacccg gcaaacttcc tgaatgttta cagtcggtcg aaaatctttt ccaaaccatt
     7201 aatgcccaca cattgaaatt ggtcaacgac gaaataaaat tttggaaaga aattgatcaa
     7261 aaaaaggcgg gagctgacaa ttatttggac gtcatgaagt cgattcaagg ggaaatcatc
     7321 aattgtacaa aaggaaaaga atgtatcctt cgattgggac atgcttcggg atggcgtttt
     7381 atcaccggtg catgggcaga aaatctgaat aatttttact ccgaagtagt gcccgctgcc
     7441 cgaccgaata atgacaaata tccagatgac gttttcccga aaacccgaag agtagaagaa
     7501 gaatatcaag atttgctggg atttgtaaaa ctaacactca ttcattaaat aaatgtttat
     7561 acctattttt attgctaaaa tctatgaaaa aacaagtatt ggtaagtatt atctccaaac
     7621 agaccattcc caattatctt tttatcaagg atagacaaaa gc
//
LOCUS       DAOSCH010000097         7251 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_6600_length_7251_cov_5.112007, whole genome shotgun sequence.
ACCESSION   DAOSCH010000097 DAOSCH010000000
VERSION     DAOSCH010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6600_length_7251_cov_5.112007"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..>2663
                     /locus_tag="PLZ54_07325"
     CDS             56..>2663
                     /locus_tag="PLZ54_07325"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOJ66772.1"
                     /translation="MQAKNLDPSPSDTLQALGKDSVLTKNDTVKVSLPKENPNKIDAE
                     ISYSASDSIVFYGNGTGFLHGKGDVKYKNITLQADFIRVKMDSSLIYARGTTDSLGNK
                     IGEPVFSEGEATYNSKELTYNLRSKKGYIRNAVTQQGEGYVISDKTKKLDNNILLIAD
                     GKYTTCDKHDNPDFYLYLTKGKIKPGDYIVTGPAYLVLADVPLPIAVPFGFFPFTDKY
                     SSGILMPNYADELNRGFGLTNGGYYFALSDYADLELLGDIYTKGTWALTANSRYIKKY
                     KYNGNFSFSYRVDVTGEKDLPDYSKATNMNIRWTHSQDQKANPYFTFTSSVNFSTSGY
                     NRSNINSYFNPNLNSENTKGSSISFTKRFPSQSNLSLSGSMLINQRTKDSTISLTLPT
                     LNIAVSRFSPFKRKNAVGNERWYEKIYMSYSGTFANSIDTKESLLLSSSLSKDWRNGM
                     RHSIPVSATFNLFKYINISPSFNYTERWYLQSVDKHWDSDKQKEITDTVSGFNRVYDF
                     NMGVSASTKLYGFFIPIRSIFGDKIDRIRHVFTPTIGFGYAPDFGDQRWGYYDSYKKT
                     TKDPSNPASMIEEEVVYSHYQGSLYGYPGSGKSGNINFSFANNIEMKLRNDNDTTGKA
                     PFKKVSLIDNFSVSGGYNLAADSMQWSNFSTNLRIKFSDKYSLNLSGAFDPYMYGLNQ
                     YGNPVRINKLRWNYHKFPKFLGTNASYSLTLNNDTFKKLFSRGDNKKTDDKKDASSKE
                     NPDNPDAESNTTSPEINQPGLSENSNQEIQTDADGYQKVTIPWNINISYSVRYANTNV
                     FDKEKMEYKMGFTHNLSFSGDISLTPKWRISSSTSYDFVAKQFTYTSINVSRDLHCWN
                     MSASFVPFGPFQS"
     assembly_gap    2664..2763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2764..3334
                     /locus_tag="PLZ54_07330"
     CDS             <2764..3334
                     /locus_tag="PLZ54_07330"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ66773.1"
                     /translation="NKSVSAKYDLFVDGENQGELELMERATEERPLSFIYGVGMMLPK
                     FEENLYGLQAGDSFEFTINDEDAYGPYDEEAVIDLDKKIFEVDGKIDENVVFEGNVVP
                     LMDTDGNRFNAQIVTVTDQLVTVDLNHPLAGQNLHFKGQVLEVRDASEAELAALMNNS
                     CSCGGNCSSEEGCDCESDSCEDPNCSCNK"
     gene            3421..4485
                     /gene="aroC"
                     /locus_tag="PLZ54_07335"
     CDS             3421..4485
                     /gene="aroC"
                     /locus_tag="PLZ54_07335"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007135861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOJ66774.1"
                     /translation="MSNTIGKFFTFTSFGESHGKAIGGVIDGCPPGIEIDEAFIQNEL
                     NRRRPGQSNISTPRQEEDKVEFLSGIFEGKSTGAPIAFVIWNENQRSADYNHLKDVYR
                     PSHADFTYQQKYGIRDYRGGGRSSARETAARVAAGAIAKLALRKMGIQINAFTSQVGH
                     IAMSKTYEDVDFSLIETNSIRCPEPETAEKMIAYIQQLKEEGDSIGGIITCVVKGVPI
                     GWGEPIFDKLQSRLAQAMLSINATHGFDYGKGFEGVALRGSQMNDEFVKDGDRITTRT
                     NNSGGIQGGISNGQDIYFRVLFKPVATISKKQNTVDIHAQEVELEARGRHDPCVLPRA
                     IPIVEAMTALTLMDLSLIKI"
     gene            complement(4465..5232)
                     /locus_tag="PLZ54_07340"
     CDS             complement(4465..5232)
                     /locus_tag="PLZ54_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOJ66775.1"
                     /translation="MKALILAAGLGTRLKPITNHIPKALVTIEGKTLLEHQILKLKSA
                     GFNEIIVNVYHFADKIIDFLHSKNNFDIRIEISDERNCLLDTGGAVRKAAWFFDDGKP
                     FLVHNVDILSNIDLMDLMKFHLSSDSLATLVVNQRKTSRQLLFDKQMQLKGWFNETTG
                     QTKPQEINLQLYQKFAFSGIQILSPKVFLLMEKWNEVFSIIDFYLSNVDKEKISGYTN
                     NEIKVLDVGKIQSLEEASIFLKNINKNFFDNSDFNEG"
     gene            complement(5265..6707)
                     /locus_tag="PLZ54_07345"
     CDS             complement(5265..6707)
                     /locus_tag="PLZ54_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOJ66776.1"
                     /translation="MNELESLFQQFTGEKVLLKEELSASGSHRRYFRLESKHFSIIGV
                     EGTSIEENRAFIEMDKLFHKKGLPVPEFYIQSQDGLYYLQEDLGNTLLFDFIAEGRKT
                     GVFCESEKEMLRKTIRKLPEIQVKGAEELDFSICYPQPEFNERSILWDLNYFKYCFLK
                     ATGIDFQENLLEDDFHRFSKILLHSQSNTFMYRDFQSRNVMIKNDEPYFIDFQGGKKG
                     PLYYDVASFLWQAKAKYSEELREELIKNYLSALAELAPVDEMEFREQLKHFVLFRILQ
                     VLGAYGFRGYYEKKPHFLESIPFAIENLRTILQNEISGYPYLLKVLRQMTELKQFREV
                     SVRKPLTVKIYSFSYKKGIPHDDSGNGGGFVFDCRAVNNPGKYERYAHVTGLDESVKQ
                     FLEEDGEILSLLDNAYKLVDASVKRYMDRGFTNLMVCFGCTGGQHRSVYSAQKMAEHI
                     NEKFGIEVQLIHREQNKEQLFPAKKLSYSK"
     gene            6978..7050
                     /locus_tag="PLZ54_07350"
     tRNA            6978..7050
                     /locus_tag="PLZ54_07350"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7011..7013,aa:Lys,seq:ctt)
BASE COUNT         2233 a         1303 c         1374 g         2241 t
ORIGIN      
        1 cagcctgaaa tgtaaattat tcctcttaat tacagggttg aatatttcat tttgtttgca
       61 agcaaaaaat ttagatccat caccttccga tactcttcaa gcattgggta aagattcggt
      121 tttaacaaaa aacgataccg taaaagtgtc tttacctaaa gaaaatccta acaaaattga
      181 tgccgaaatt agttattctg cttccgattc tattgttttt tacggaaatg gaacaggttt
      241 tttgcatgga aaaggcgatg tgaagtataa aaacataaca ttgcaggccg attttattcg
      301 tgttaaaatg gatagcagcc tgatttatgc cagaggaacg actgattcgt tgggaaataa
      361 aattggagaa cctgttttca gtgaaggaga agcaacttat aattcgaaag agctgaccta
      421 taatttacga agcaaaaaag gatatattcg aaatgcagtt acccagcagg gagaaggtta
      481 tgtaatcagc gataaaacaa aaaaactgga caataatatt ttgctgattg ccgacggtaa
      541 atatactacc tgcgacaagc atgataatcc cgatttttat ctttatttga ccaaaggaaa
      601 aataaaacca ggagattaca ttgttacggg gcctgcttac cttgtgttgg ctgatgttcc
      661 tttaccaatt gctgttcctt ttggtttttt cccttttacc gacaaatatt cttcgggtat
      721 attaatgccc aactatgctg atgaactgaa tcgcggtttc gggttgacta atggaggcta
      781 ttattttgcc ttgagcgatt atgccgactt ggaattgttg ggagatattt atacgaaagg
      841 aacttgggct cttactgcca attcacgata cataaaaaag tataaatata atgggaattt
      901 cagttttagc tatcgggtgg atgttaccgg cgaaaaagat ttacccgatt acagtaaggc
      961 gaccaatatg aatattcgtt ggacacattc gcaggatcag aaggcaaatc cgtattttac
     1021 ctttacatcc agtgttaatt tttcaaccag tggttacaac cgaagtaata tcaacagcta
     1081 ttttaatcca aatttgaatt cggaaaacac caaaggttcc agtatatctt ttacaaaaag
     1141 atttccttct caatccaatc taagtttgtc gggaagtatg ttgataaatc aacgtacaaa
     1201 agattccacc atcagtctta cattaccgac tcttaatatt gctgtcagcc gtttttctcc
     1261 attcaagcga aaaaatgctg ttggtaatga aagatggtac gaaaaaattt atatgtccta
     1321 ttcgggtact tttgccaata gcattgatac aaaagaatct ttgctgttgt catcttcgct
     1381 ttccaaagac tggcgaaacg gaatgcggca cagtattccg gtgagcgcta ccttcaattt
     1441 attcaagtat ataaacattt ctccttcttt caattacact gaacgctggt atttacaatc
     1501 tgtggataag cattgggata gcgataaaca aaaagaaata accgatacgg ttagcggttt
     1561 taacagagtt tacgatttta atatgggcgt ttcggcttct accaaacttt atggcttttt
     1621 tattcctatt cgttccattt ttggagataa aattgaccgt attcggcatg tttttactcc
     1681 aacgatcggt ttcggatatg ctcccgattt tggtgatcag cgttggggat attacgattc
     1741 ctacaaaaaa acgaccaaag atccttccaa tcctgccagc atgattgaag aagaagttgt
     1801 atattcccac tatcagggaa gtttgtacgg atatccgggt tcaggtaagt cgggaaatat
     1861 caatttttca tttgccaaca atattgagat gaaactcaga aacgacaatg atacaacagg
     1921 gaaagcacct tttaaaaaag tcagtctgat tgataatttt tcggtaagtg gaggatataa
     1981 tctggcagcc gattccatgc aatggtcaaa ttttagtacc aatcttcgaa ttaaattcag
     2041 cgacaaatat tcgttaaatc ttagtggagc atttgaccct tatatgtatg gattaaatca
     2101 gtatgggaat ccggtcagaa tcaataaatt gcgttggaat tatcataaat ttcccaaatt
     2161 tctcggaaca aatgcctctt atagcctgac tctgaacaac gatactttca aaaaactttt
     2221 ctcgcgcgga gacaacaaaa aaacagatga caagaaagat gcttcctcga aagaaaatcc
     2281 agataatcct gatgctgaaa gtaacacgac ttcaccggaa ataaatcagc cgggactttc
     2341 ggaaaactca aatcaggaaa ttcagaccga tgctgatggt tatcaaaaag tgaccattcc
     2401 gtggaatatc aatatcagtt attctgttcg atatgccaat acaaatgttt tcgacaagga
     2461 aaaaatggaa tacaaaatgg gatttaccca caacttaagc ttttcgggag acatttcgct
     2521 gacgcccaaa tggagaatca gctcgagtac ttcctatgat tttgttgcaa aacaatttac
     2581 ctacaccagt attaatgttt cacgcgattt gcattgttgg aacatgtcag ccagttttgt
     2641 tccttttgga ccttttcagt cctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnaaacaaa tcagtgtcag ctaaatacga tttatttgtt gacggagaaa atcaaggtga
     2821 attggaatta atggaacgtg caaccgaaga acgtccgctg agttttattt atggagtcgg
     2881 aatgatgctt cccaaatttg aagagaattt atacggattg caagccggcg attcttttga
     2941 attcaccata aatgatgaag atgcatatgg cccttatgat gaagaagcag ttatcgattt
     3001 ggacaaaaaa atcttcgaag tggatggaaa aattgatgaa aacgtagtat ttgaaggaaa
     3061 tgttgttcct ttaatggata cggatggcaa tcgatttaat gcccaaattg ttacggtaac
     3121 cgatcaactt gttacggtag atttgaatca tccgttggca ggtcagaatc tgcatttcaa
     3181 aggtcaggtg ttggaagtac gtgatgcttc agaagccgaa ttggccgctt taatgaacaa
     3241 ttcgtgcagt tgtggcggta attgctcttc tgaggaaggt tgcgactgtg aaagcgacag
     3301 ttgtgaagat cccaattgct cttgcaataa gtaattttat ttttgaattt taagcaatgg
     3361 aaaaggttat acagcctttt ccattggatt ttctttttca gtaataacga ttttttagtt
     3421 atgtcgaaca ccattggcaa gttttttaca tttacctctt ttggcgaatc gcacggcaaa
     3481 gccattggag gcgttattga tggatgtccg ccgggaatag agatagatga agcgttcatt
     3541 cagaacgaat tgaatcgtcg tcgtccggga caatccaata tctcaacgcc cagacaagaa
     3601 gaggataagg ttgaatttct ttcaggaatt tttgaaggaa aatctacagg agcgcctatc
     3661 gcttttgtga tttggaatga aaatcaaaga agtgccgatt acaatcatct gaaagatgtt
     3721 tatcgacctt cgcatgccga ttttacttat cagcaaaaat atggcattcg cgattatagg
     3781 ggaggtggca gaagctctgc acgtgaaacg gcagccagag tagcagcagg cgcaattgcc
     3841 aagttggctt tgcgaaaaat gggcattcaa atcaatgctt ttacttcgca agtaggacat
     3901 attgccatga gcaaaactta cgaagatgtt gatttttcac taatcgagac caattctatt
     3961 cgttgtccgg aaccggaaac tgccgaaaag atgatagctt acatccaaca gttaaaggaa
     4021 gaaggtgatt ctataggtgg catcattacc tgcgtagtca aaggtgttcc gattggctgg
     4081 ggagagccga ttttcgacaa acttcaatcg cgtttggcac aagccatgtt aagcataaat
     4141 gcaactcacg gtttcgatta cggaaaaggt tttgaaggtg tggcgctcag aggttcgcaa
     4201 atgaatgacg aatttgtgaa agatggcgac agaatcacta cgcgaaccaa taattccgga
     4261 ggcattcagg gaggaataag taacgggcag gatatatatt ttcgtgtcct tttcaaacca
     4321 gtggcaacca tttcaaaaaa gcaaaatacg gtagatattc atgctcagga agtggaactg
     4381 gaagctcgcg gacgccatga tccttgcgtt ttgccacgtg ctattccgat agtagaagcc
     4441 atgacagcac ttactttgat ggatttatcc ctcattaaaa tctgaattat cgaagaagtt
     4501 tttattaata tttttcaaaa aaatgctggc ttcctctaaa ctttgtattt ttcctacatc
     4561 cagcactttt atttcattat tggtgtagcc cgaaattttt tctttatcca cattactcaa
     4621 ataaaagtcg ataatggaaa aaacttcatt ccatttttcc atgagtagaa aaacttttgg
     4681 cgataaaatc tgaattccgg aaaaggcaaa tttctgataa agctgtaaat ttatttcctg
     4741 cggtttggtt tgtccggtcg tttcattgaa ccaccctttc agctgcattt gtttgtcgaa
     4801 gagcaattga cgtgaggttt tacgttgatt taccaccaat gttgccaagc tgtcgctgct
     4861 tagatgaaat ttcatcaagt ccattaaatc tatattggaa agaatatcga cattgtgtac
     4921 caaaaaaggc tttccgtcat caaaaaacca tgcagctttg cgtaccgctc ctcctgtgtc
     4981 gagtagacaa tttctttcgt ctgaaatttc gatccgaata tcaaaattgt tctttgaatg
     5041 aagaaaatcg ataattttat cggcaaaatg ataaacattc acgattattt cgttaaaacc
     5101 tgccgatttc aatttcaaaa tttgatgttc gagtaaagtt tttccctcta tcgttaccag
     5161 cgctttagga atatgattgg taattggttt aagtcgcgta cccagtcccg ctgccagtat
     5221 caatgctttc attcagaatt aaagattttt tgaaatataa aacgttattt ggaatagctt
     5281 aatttttttg cagggaaaag ttgttcttta ttttgttctc tatgaattag ttgtacttct
     5341 atgccaaatt tttcgttgat atgttctgcc attttttgtg ccgaataaac cgatcgatgt
     5401 tgtcctccgg tacatccaaa acaaaccatt aaattggtaa atcctcgatc catgtagcgt
     5461 tttaccgaag catccacaag cttgtaggca ttatcgagca aagatagaat ttcaccatct
     5521 tcttccaaaa attgtttcac cgattcgtcg agtcctgtaa catgagcata tcgttcgtat
     5581 ttgcccggat tgttgactgc tctgcaatca aatacaaagc cgcctccatt tccgctatca
     5641 tcatgaggaa tgcctttttt ataggaaaag ctgtaaattt taactgtcag tggcttcctt
     5701 actgaaactt cgcggaattg ctttaactcg gtcatttgtc tgagcacttt caaaaggtaa
     5761 gggtaacctg aaatttcatt ttgaagaatg gttcttaaat tttctatggc aaagggaatg
     5821 ctttccagaa aatgaggttt tttctcataa tagccccgaa aaccataagc tcccaacact
     5881 tgtaaaattc taaaaagaac aaaatgtttt aattgttctc taaactccat ctcatctaca
     5941 ggtgccagtt ctgctaatgc cgaaagataa ttttttatga gttcttcgcg caattcttca
     6001 ctatatttgg ctttagcctg ccataggaac gatgctacat cataatacaa tggccctttt
     6061 ttcccacctt gaaagtcgat aaaataaggt tcatcgtttt ttatcattac atttcggctc
     6121 tgaaagtcgc gatacataaa tgtattggac tgtgagtgca acaaaatttt ggaaaaacga
     6181 tgaaaatcgt cttccagcaa attttcctga aaatcgatac cggttgcttt cagaaaacaa
     6241 tatttaaaat aatttaaatc ccataaaatg gaacgttcat taaattccgg ctgggggtag
     6301 caaatggaga aatccagttc ttccgctcct tttacttgaa tttcgggaag ttttcgaatt
     6361 gttttacgaa gcatttcctt ttccgattca caaaacacac ccgtttttct cccttctgct
     6421 ataaaatcga aaagcagcgt attacccaaa tcctcctgca aataatacaa accatcctga
     6481 ctttgaatgt aaaactccgg aaccggcaat ccttttttgt gaaacaattt atccatttct
     6541 ataaaagccc tgttttcttc aatggaagtt ccttctactc cgataataga aaagtgcttg
     6601 ctctcgagtc taaaatatcg tctgtgcgat ccggaagccg acaattcttc tttcagaaga
     6661 actttttctc cggtaaattg ttggaaaaga gattctaatt cattcatttt tagaattttt
     6721 tttaattaat gcaggttaca caaagatata aaaagaaata tacatctttg cttgaatttt
     6781 taagcgcgtt aaattataaa acgaagtaaa ttgttcaaat tctataaata tttgaatatt
     6841 tggtagttga ttaagaaaaa tttgcactaa atattttttt aaaattcaaa atgaaaaaat
     6901 ttcaaaaatc tttctatctt tgcaatacaa aaaggagaga agtaagtttg ttcttacact
     6961 tttttgaaat ataaaacgac tccgtagctc agctggtaga gcaacagact cttaatctgt
     7021 gggtcgtggg ttcgatcccc accggtgtca ctattcctgg tttaaaatat taaaaataat
     7081 ctttaaaatt ctacagacat gaaaagcaga aatgttttta tgatttatgt gatgagtgca
     7141 gcaattattt tttcaggatg caaatcgatg agtaaaaccg ccagtggagc tttgatagga
     7201 agcggtgcgg gtgctgccgt aggtgccgga attggcgcat tgataggtaa a
//
LOCUS       DAOSCH010000098         7235 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_6619_length_7235_cov_4.929248, whole genome shotgun sequence.
ACCESSION   DAOSCH010000098 DAOSCH010000000
VERSION     DAOSCH010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7235
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6619_length_7235_cov_4.929248"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..2085)
                     /locus_tag="PLZ54_07355"
     CDS             complement(61..2085)
                     /locus_tag="PLZ54_07355"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOJ66777.1"
                     /translation="MNSIELLNKAADNIRILAAAMVEKAKSGHPGGAMGGADFMNVLF
                     SEFLEYDPENPTWESRDRFFLDPGHMSTMLYSVLAFSGKFTLDELAQFRQWGSPTPGH
                     PELDVMRGIENTSGPLGQGHTYAVGAAIAAKFLKARFGDVMNQTIYAYISDGGVQEEI
                     SQGAGRIAGHLGLDNLIMFYDSNNIQLSTETKAVTSENTAEKYKAWGWKVIEIDGNDA
                     VQIRQALTEAKAEKERPTLIIGKTVMGKGIRKADGSSFENQTSTHGMPISECGASFEE
                     TIRHLGGDPEHPFVIFEETKALYEKRKNELKEIVARKKTAKEAWAKEHPDLAKKLESF
                     FSGKAPEVDWEAVVQQPNKSTRAASATVLGELAKQVENMITASADLSNSDKTDGFLKK
                     TKAFSKNDFSGSFLQAGVSELTMACLCIGMSLHGGVVPACGTFFVFSDYMKPAVRMAA
                     LMEQPVKFIWSHDAFRVGEDGPTHQPVEQEAQIRLMEKLKNHHGRNSMLVLRPADVEE
                     TTVAWKMALENIKTPTGLILSRQDIKDLPSTNGRKEEAKKAEKGAYVVQCDGNPDIIL
                     LASGSEVSTLNEAAVLLRNDGIRVRIVSIPSEGLFRLQSKEYQEMVLPQEVKKFGLTA
                     GLPVTLQGLINNNGKVFGMESFGFSAPYKVLDQKLGFTGENIYHQIKLML"
     gene            complement(2132..2836)
                     /locus_tag="PLZ54_07360"
     CDS             complement(2132..2836)
                     /locus_tag="PLZ54_07360"
                     /inference="COORDINATES: protein motif:HMM:NF039966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6048 family protein"
                     /protein_id="HOJ66778.1"
                     /translation="MFQPSMQNIYRYFIISLFVAVAVNFSTAQNKTPASAKSPEVKWW
                     NGFLVKMDVASPVITGFSDTEHFGMEGSVQFNLKRKIFPVYEIGFGGADKLSSNEVRF
                     NTNGIFQRIGIDFGLLKQSSDKELINNMLTGGVRLGWSNFSYDLTNLSLSDDYWGGSQ
                     TLNFEGQSATKTWYEIVVGIQVQVYKNFYLGWNIRNRHFFKEDTSGKIAPWYIPGYGK
                     TGSSNTGFNYTIGYHF"
     gene            complement(2790..3356)
                     /locus_tag="PLZ54_07365"
     CDS             complement(2790..3356)
                     /locus_tag="PLZ54_07365"
                     /inference="COORDINATES: protein motif:HMM:NF040109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6452 family protein"
                     /protein_id="HOJ66779.1"
                     /translation="MIFINFKNLFDTLKNFLHNNFTKSTVFIWITGVLFFSTSCNVSD
                     EVCRKEKNARLKVGFYTAVFNSSTGKYTSSSLTIDSLTVNGVDSLGSIVDSILYNNSK
                     NVSVIKLPLNKFSNESKFIIRFNNSIQDTLIVFHTNYDQYLSFECGMIRNHLIDTVLI
                     TNHFVDSVKIEEHNVSTIDAEHLQIFHN"
     gene            complement(3390..3908)
                     /locus_tag="PLZ54_07370"
     CDS             complement(3390..3908)
                     /locus_tag="PLZ54_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66780.1"
                     /translation="MAEILKLLIPALLVLLTAYLLLDRLLKNEEKRRNFELFKSNYSV
                     ITPIRLRAYERLMLVLERTIPNNLILSTIKPGMTNLELQSQLLNTIRHEFAHNLSQQI
                     YVSDELWNYISSAQESLLRLINMCASQCNPNEPASLLAEKILEIYAASEETPTEQAIQ
                     KLKEEVRKYFPS"
     gene            complement(3937..4290)
                     /locus_tag="PLZ54_07375"
     CDS             complement(3937..4290)
                     /locus_tag="PLZ54_07375"
                     /inference="COORDINATES: protein motif:HMM:NF014677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HOJ66781.1"
                     /translation="MKKNENSILKIYASSTDKMGNELLYEHLVFLAKENGIAGVTVYR
                     GIMGYGLSSQKISTSRFWELTEKLPVVVEMMDTTDKLESFYQTIEEKLKNIPNGCLVT
                     LQPVEVKLHKFGNKK"
     gene            complement(4292..4666)
                     /gene="crcB"
                     /locus_tag="PLZ54_07380"
     CDS             complement(4292..4666)
                     /gene="crcB"
                     /locus_tag="PLZ54_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HOJ66782.1"
                     /translation="MKQLLIVGLGGFFGTMARYLVSRINLLENFLSLPLGTLTVNVSG
                     SFLIGILTGLASKSELLSADMRLFLMAGFCGGFTTFSSFTNENLQLIQNGQFLSAAVY
                     TVISLLLGFLAVFLGYAVTNFL"
     gene            complement(4737..5690)
                     /locus_tag="PLZ54_07385"
     CDS             complement(4737..5690)
                     /locus_tag="PLZ54_07385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ66783.1"
                     /translation="MDISVVVPLYNEEESIRPLYEWIERVMNENGFSYEVIFVNDGST
                     DRSWEIIESLKASSPEHVHGIKFRNNYGKSVALHFGFQAAKGDVVITMDADLQDSPDE
                     IPSLYDMIKNQNYDLVSGWKKKRYDAKLTKNFPSKIYNATARRVTKLQLHDMNCGLKA
                     YRNEVVKSIELYGEMHRYIPYLAKIAGFTKIGEKVVEHRKRQFGTTKFGWNRFVNGYL
                     DLMTLWFLSKFGKQPMHFFGLIGTLIFLLGLIAVIVVGAKKLIALSAHVSAPLVTSSP
                     YFYLAILAMILGTQLFLAGFVAELITRNSSERNNYLIEKEI"
     gene            complement(5814..6320)
                     /locus_tag="PLZ54_07390"
     CDS             complement(5814..6320)
                     /locus_tag="PLZ54_07390"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOJ66784.1"
                     /translation="MQGNIFKSSMIYGLMMGILFSVNFIISIPQGAIFRIVSFFITIV
                     IVIVMYRYSVKYRDNECGGYITYGKSFSFIVLTFFFASIISAIVKFVYFQFINPEYLS
                     NVLQQSMKVVESMNLPLDDEYYTQMEKMMKPSVYTLQSIWVNVLLGALLALVMSAFVK
                     KDKDIFEE"
     gene            6668..>7235
                     /locus_tag="PLZ54_07395"
     CDS             6668..>7235
                     /locus_tag="PLZ54_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HOJ66785.1"
                     /translation="MDKTQKQILEAQRTELTEHLIYKKLADDCKDENNAKILYEIGQQ
                     ELEHAHYWEKKTGIKVKPDHARIRRTVFLARLLGLSFVLKQMEKREGTGSKLYEELST
                     VYPETKLFSEQENAHEQQLLAMLDEERLQYVGSIVLGLNDALVELTGALAGFTLALGN
                     TRTISLAGLVTGISAALSMAASDYLSTKA"
BASE COUNT         2239 a         1492 c         1317 g         2187 t
ORIGIN      
        1 atcgcaatat ccaataaaaa agagactgtc tctacttttg agacagcctc tttttcgctt
       61 ttaaagcatc aattttatct gatgataaat attttctccg gtaaatccaa gcttttgatc
      121 caagacctta tatggagcag aaaagccgaa agattccatt ccgaatactt ttccattgtt
      181 gtttatcagc ccttgcaaag ttacgggtaa ccccgcagtg agtccgaatt tttttacttc
      241 ctgaggcaaa accatttcct ggtattcttt tgactgaaga cggaacaatc cttcggaagg
      301 aatggaaaca atccgcaccc gaattccatc attgcgaagt aaaacggcag cttcattcaa
      361 agttgaaact tccgatccgg aagcaagcag aatgatatcg ggatttccgt cgcactgaac
      421 aacataggcg cctttttcag cttttttggc ttcttcttta cgaccatttg tggacggaag
      481 atctttaata tcctgacgtg aaagtatcaa gccggtaggc gttttgatgt tttccagtgc
      541 cattttccaa gcaacggttg tttcttcaac gtcagccgga cgaagaacca gcatggaatt
      601 tcgtccgtga tgatttttaa gtttttccat taagcgtatt tgtgcttcct gctccaccgg
      661 ctgatgagtt gggccatctt cacccacacg aaaggcatca tgcgaccaga taaacttcac
      721 cggttgctcc attaatgcag ccatgcgaac ggcaggcttc atgtagtcgg aaaatacgaa
      781 aaaggttccg caagccggaa caacaccgcc gtgtaaactc atgccgatac aaaggcaagc
      841 catagtcagt tccgacacgc cggcctgtag aaatgaacct gaaaaatcgt ttttggaaaa
      901 agcttttgtt tttttcagaa aaccatctgt tttgtcggaa ttggaaagat cggcactggc
      961 agtaatcata ttctctactt gtttggccaa ttcgccaaga actgttgccg aagctgcacg
     1021 agtggatttg ttaggttgtt gaacaacagc ctcccagtca acctcaggag cttttcccga
     1081 aaagaatgat tcgagttttt ttgccaaatc aggatgttct tttgcccaag cttctttggc
     1141 tgtttttttt ctggctacta tttctttcaa ttcgtttttg cgcttttcat aaagtgcttt
     1201 tgtctcttca aaaattacaa acggatgctc agggtcgcca cccaaatgtc taatggtttc
     1261 ttcaaaagaa gcaccacatt cgctgatcgg cataccgtgt gtggaagtct gattttcgaa
     1321 actactgccg tcagccttcc gaattccctt gcccatgacg gttttaccga ttattaacgt
     1381 aggccgctcc ttttctgctt tggcttccgt taaagcttga cgaatttgca ccgcatcatt
     1441 tccatctatt tcgataactt tccagcccca agctttgtat ttttcagccg tattttcact
     1501 ggtaactgcc tttgtctcgg tagaaagttg aatattattg gaatcgtaaa acataatcag
     1561 attgtccaat cccaaatggc cggcaattcg ccctgctccc tgtgaaattt cttcctgaac
     1621 accgccatcg gaaatatagg cataaatggt ttgattcatt acatcgccaa agcgtgcttt
     1681 taaaaatttt gctgcaatgg ctgctccaac agcataagta tgcccctgtc ccagtggtcc
     1741 ggaagtgttt tcgatgccgc gcattacatc caattcgggg tgacccggag ttggacttcc
     1801 ccattgacga aactgtgcca attcgtccaa tgtaaatttt ccagaaaaag ccagaacgga
     1861 atacaacatg gtggacatgt gtccgggatc aagaaaaaac ctatcgcgac tttcccatgt
     1921 aggattttca ggatcgtact ccagaaattc ggaaaaaagt acgttcataa aatcagcacc
     1981 gcccattgcc ccgccgggat gacccgattt ggccttttct accatagctg ctgccaaaat
     2041 acgtatgttg tcggctgctt tgtttaaaag ttcaattgaa ttcataataa aaaaattttg
     2101 ttgttaggtt gtgtttgtta ttcatgtttt tttagaagtg atatccgatg gtataattaa
     2161 agcctgtgtt ggaacttccg gttttcccat agcccggaat gtaccacgga gcgattttgc
     2221 cggaagtgtc ttccttaaaa aaatgccggt tcctgatatt ccaccccagg taaaaatttt
     2281 tgtaaacctg tacctgtatt cctactacaa tctcgtacca cgttttggtt gcggactgac
     2341 cctcaaaatt taatgtttga ctgccacccc aataatcatc cgaaagagaa aggttagtta
     2401 aatcgtagct gaagttggac catcccaaac gaactccgcc cgtaagcata ttgttaatta
     2461 attctttatc actactttgt ttcagtaaac cgaaatcaat gcctattctt tgaaaaattc
     2521 cgttggtatt aaaacggact tcattggatg aaagtttatc ggctccacca aaacctattt
     2581 cgtaaacagg aaaaattttt cttttcaaat tgaactgaac actcccctcc atgccgaaat
     2641 gttcggtatc ggaaaatcct gtaattaccg gagacgccac atccattttg acaagaaacc
     2701 cattccacca cttcacttca ggtgatttgg ccgatgccgg ggttttattc tgtgcggtag
     2761 aaaaatttac agccacagca acaaacaaac taattatgaa atatctgtaa atgttctgca
     2821 tcgatggttg aaacattatg ttcttcaatt tttaccgaat caacaaaatg attggttatc
     2881 agaaccgtat ctatcaaatg atttctgatc attccacatt caaacgacaa atactgatca
     2941 taattggtat ggaaaacgat aagtgtatcc tgaatgctat tgttgaaacg aataataaat
     3001 ttcgattcgt ttgaaaattt gttgagcggc aatttaatga cagaaacatt tttagaattg
     3061 ttgtaaagaa tagaatctac gatactgccg agagaatcaa ctccgtttac cgtaaggctg
     3121 tcgatggtaa gcgacgacga agtgtacttg ccagtggaac tgttgaaaac ggcagtataa
     3181 aaaccaacct tgagccgggc attcttttct tttcgacaaa cttcatcgga aacattacat
     3241 gatgtcgaaa aaaacaaaac accagtaatc caaatgaaaa ctgttgattt ggtaaagttg
     3301 ttatgaagaa aatttttcaa agtgtcgaat aaattcttaa agttaataaa aatcattgtt
     3361 accgctttct cttttcaaag ttgtttgcat taggatggaa aatattttct cacttcttcc
     3421 ttcaattttt gaatagcttg ctcggtgggc gtttcttcac tggctgcata aatttccaga
     3481 attttttcgg ccagcaaaga agcaggttca ttcggattgc attgcgaagc gcacatatta
     3541 atcaaacgaa gtaaactttc ctgggctgaa gaaatataat tccaaagttc atcactcacg
     3601 taaatttgtt gcgaaagatt atgcgcaaat tcatggcgga tagtgttcaa caattgcgac
     3661 tgaagttcca agttggtcat gccgggtttt atggtgctta atatcaaatt atttggaatt
     3721 gtacgctcca aaacaagcat taatctttcg taggctctca aacgaatggg cgtaataaca
     3781 gaataattgc ttttaaacaa ttcgaaattt cgccgttttt cttcgttttt caaaagtctg
     3841 tccagcaaaa ggtaagctgt cagcaaaacg agcagcgcag gaatcagcaa tttcaatatt
     3901 tcggccatga agattggtat gtttttagaa aatattctat tttttattcc caaatttgtg
     3961 cagtttcacc tcaaccggct gcagagtaac cagacaacca ttcggaatat ttttcaattt
     4021 ttcttctatc gtttgataaa agctctcaag tttgtcggtc gtatccatca tttcaaccac
     4081 aacggggagt ttttccgtca gctcccaaaa acgcgaagtt gaaatttttt gactgctcaa
     4141 cccataaccc ataattccgc gataaaccgt tacgccggca atgccgtttt cctttgccag
     4201 aaaaaccaga tgttcgtaca gcagctcgtt tcccatttta tccgtactgc tggcataaat
     4261 tttcaatatg ctgttttcgt tttttttcat tttacaagaa attggttact gcatagccaa
     4321 gaaaaaccgc aagaaaacct aacaaaagac tgatgaccgt ataaacggct gcactcagaa
     4381 attgtccgtt ttgaatcagc tgcaaatttt cattggtaaa ggacgaaaaa gtcgtaaaac
     4441 ctccacaaaa tcctgccatc agaaacaatc gcatgtcagc agataataat tcacttttgc
     4501 tggccaaacc cgttaaaatt ccaatcaaaa aactgccgga aacatttacc gtgagcgtac
     4561 ccaacggtaa ggataaaaaa ttttccagca aattgattct ggaaacaaga tagcgagcca
     4621 ttgttccgaa aaaaccacct aatccgacaa tcagcaattg cttcatattc taaatatttc
     4681 cttttttgca aggtactaac aagctccctg tatccgagcc aatgaaagcc attttcttaa
     4741 atttcctttt cgatcagata gttgtttctt tccgacgaat ttcgtgtaat cagttcagca
     4801 acaaaacctg ccaggaagag ctgcgttcct aaaatcatag ccagaatggc cagataaaaa
     4861 tacggcgaac ttgtaaccag tggagcagaa acatgtgccg acaaggcgat aagtttttta
     4921 gcacctacca caatcaccgc tatcaagccc aacaggaaaa tcaacgtccc tatcaaacca
     4981 aaaaagtgca tgggctgctt cccgaatttt gacagaaacc aaagtgtcat caaatcgaga
     5041 taaccgttta caaaacgatt ccagccaaat ttggtcgttc cgaactgacg cttacgatgt
     5101 tccaccactt tttcgccaat ttttgtaaaa ccggcaattt tggcaagata aggaatatat
     5161 cgatgcattt cgccatacaa ttcgatactt tttaccactt cattgcgata agctttcagt
     5221 ccgcaattca tgtcgtgaag ttgaagtttc gtaacacgtc gagctgttgc attatatatt
     5281 ttagaaggaa aatttttggt aagtttggca tcataccgct tctttttcca acccgaaacc
     5341 aaatcataat tttgattttt tatcatatcg tacaaagacg gaatttcgtc gggactatcc
     5401 tgcaaatccg catccatagt aatcaccaca tctcctttgg ctgcctgaaa tccaaagtgc
     5461 aaagctaccg atttaccata attgtttctg aattttatcc cgtgaacatg ctcgggagaa
     5521 gaggctttca acgattcgat aatttcccat gaacgatcgg tacttccatc attcacaaaa
     5581 attacttcgt aactaaagcc attttcattc attactcgct caatccactc gtaaagagga
     5641 cgaatcgatt cttcttcgtt atataaaggt acaacaactg atatgtccat ttttaaattg
     5701 agttttataa attggctttt taaatcacta ctggatcaaa taatgcaatt ttaaaaaaat
     5761 catttaagca ttgatataga agaaacaatt tgacaaaaat caatagatat tttttattct
     5821 tcaaaaatat ccttatcctt tttcacaaat gccgacataa cgagcgccag caaagctccg
     5881 agcaatacgt ttacccaaat gctctgcaaa gtataaaccg aaggtttcat cattttttcc
     5941 atttgggtgt aatattcatc gtccaaaggc aaattcatac tttctactac tttcatgctt
     6001 tgctgcaaaa cattggaaag atattcggga ttgataaact gaaaataaac aaatttaaca
     6061 atggccgaaa taatggaagc aaagaaaaaa gtaagtacaa taaaggaaaa cgatttcccg
     6121 taagtgatat aaccgccaca ctcgttatcg cgatacttga ccgaatagcg atacataaca
     6181 atgacgatca caatggttat aaaaaatgag acaattctga aaatggcacc ctgagggatg
     6241 gaaataatga aattgacgga aaaaagtatt cccatcatta atccatatat catggaagat
     6301 ttgaaaatat ttccctgcat gattttattt tttacattta ctcatgcaaa agtacaaaaa
     6361 aagacctgaa tcggaaatat taaatttttc agttcaatta taagtcgaga aaacaacact
     6421 taaattcaat ttgtattagc ccgtaataac cgagcacatt gattgtattt ttttttatta
     6481 ttttttacta ttcggttttt ttgagggcta atatagaaca taatgatgat tttgttgtta
     6541 aagaaatatg atgtttatgc agaaaataca atttggaaag agaattccaa caaaattata
     6601 taatctctaa tgaggatttt tgttttaatt ttaatgataa gaaatcaaat tttagtactc
     6661 aaaaattatg gacaaaacac aaaagcaaat tttagaagct caaaggactg aactgaccga
     6721 gcatttgatt tacaaaaaac tcgccgatga ttgtaaagat gagaataatg caaaaatttt
     6781 gtatgaaatc ggacaacagg aactcgagca tgcccattat tgggaaaaga aaaccggtat
     6841 caaagtgaaa cctgaccatg ccagaattcg tcgaacagta tttttggcgc gtttgttggg
     6901 actctctttt gtactgaagc aaatggaaaa aagagaagga acgggttcaa aattgtatga
     6961 agaattgtcg accgtttacc ctgagaccaa attgttttcc gagcaggaaa atgctcatga
     7021 acagcaattg ctggccatgc tggatgaaga aaggctgcaa tatgtcggtt ccatcgtatt
     7081 gggattaaat gatgcgttgg tagaattgac tggagctttg gcaggcttta cgctggcttt
     7141 gggaaataca agaactattt cgctggcagg gttggttaca ggaatttctg ctgcgctttc
     7201 tatggctgct tccgattatc tttctacaaa agccg
//
LOCUS       DAOSCH010000099         7043 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_6867_length_7043_cov_5.579994, whole genome shotgun sequence.
ACCESSION   DAOSCH010000099 DAOSCH010000000
VERSION     DAOSCH010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7043
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6867_length_7043_cov_5.579994"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1531)
                     /locus_tag="PLZ54_07400"
     CDS             complement(<1..1531)
                     /locus_tag="PLZ54_07400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOJ66786.1"
                     /translation="MKKNVKLLIVLVFQLFAFGQINAQIVKGIVTDAKGEAIIGATVL
                     EKGTNNGTVTNVNGEFSLTLKNANSMIEVSYIGYEKQSLSHTGNGLIKVVLNENAKAL
                     DEVVVTALGIKREKKSLGYAIQEVKGDDLTKVKSNNVMNSLAGRVAGLKVSTTGTGEW
                     GTTNILLRGATSLSGNNQPLIVVDGVPVSGDSRGTGSENGGIDYGSGLSDINPDDIES
                     ISMLKGGNAAALYGSRAAAGVIMITTKKGIVKKTLGVKYSYDLGISTLNVMPDLQNLY
                     GRQTEIVDGVEQEVLKGTNWADSKLDGRMVKKWNGDIAPFTAQPNNIRDFFQTGIRNT
                     HSIEMDGGSDIAQARLSTMVSNSKGIIPNSELKKYSFSLSGNAKLGKKLTISGRATYL
                     IQNTQNRPNLADSPDNPMYSFLRMPRNIQLDDMRDYEDVNGFPRLWDGLAKSYTDDIS
                     KNQNPFWSVYKNINKDNRYRLMANVSAKYEFTKNLDLLVRAGTDYILDDNETRVAKRT
                     AY"
     gene            complement(1536..3725)
                     /locus_tag="PLZ54_07405"
     CDS             complement(1536..3725)
                     /locus_tag="PLZ54_07405"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOJ66787.1"
                     /translation="MISKTKILTVILLITCHLQAVDFTRYVDPMIGSDYHGNVFVGAS
                     VPFGMVQLGPTNMSKGWHWCSGYHYSDSTIIGFAHTHLNGTGIGDLGDILFMPTTGTP
                     TTFKGTFDDLSKGYVSIFSHSNEKVQPGYYSVLLQKYGIKAELTATSRVGYHRYTFPE
                     SVESNIIIDLGEGIDWDEPTETMLKQIDARTIEGYRYSRGWAKNQKIYFTAVFSKDMN
                     FTKVVEPVFRPRSNDSIDVQKAVLSFETKKNEAVEVKVAISYVSCKNAANNLIAELPN
                     WNFKETLAQAQAKWNTELSKIRIKTNDEKVRTMFYTSLFRTMISPSDFCDVNGDYRGA
                     DGKVYKANGFVPHTIFSLWDTYRTFHPLQTIINQKRTADWVNSLLDITEKQGSLPIWH
                     LVGNETGTMVGVHSIPVIVDAVLKNIENIDAERAYNLVRGFEQRNDRGLKFVREQGWI
                     PADKENWSVSKGLEYAIDDYAIALLAKHLGKEDDYQLFSKRAKYYANYFDKELGFMRG
                     KLADGTFRKGYNPAFSLHEEADYVEGNGWQYTFLVPHDVEGLIELFGSKQKFTDKLDS
                     LFTVSSVLNEGASIDISGLIGQYAHGNEPSHSTIYLYQYVDRADKTAERVRQVLEEMY
                     KPTPAGLCGNDDCGQMSAWYVMSALGIYPMNPVNGEYVFGSPIVHNAVIQLENGNKFI
                     IEAKNNSEKNKYIQSVKINGKMVYGKAISFNQLMNGGKLEFRMSDKK"
     gene            complement(3722..5521)
                     /locus_tag="PLZ54_07410"
     CDS             complement(3722..5521)
                     /locus_tag="PLZ54_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ66788.1"
                     /translation="MDVLVVGGGTSGTAAGIQAARMGALTLIVEETPWLGGMLTSAGV
                     SAMDGNHQLPSGIFGEFIDSLVAHYGSADALKTGWVSNVLFEPKVGNAIFQKMTKTEK
                     KLTVWQNSKFISATKIKHGWKVKVSKGGVIQSVTTKIMIDATELGDVAKHCGVKYDIG
                     MEARSVCGEDIAPEQSNDIIQDLTYVMILKDYGFDKTITKPASYDASLFYCTAKSSNC
                     TNPKPGQTLWPKEKMMTYGKLPNGKYMINWPIEGNDFYVNMLEMNDAERTVALEKAKN
                     FSLCYLYYLQTELGFSTYGLDEEQFPTADHFPLIPYHRESRRIHGLVRFNVNHVVKPF
                     SQPEALYRTGIAVGDYPIDHHHNRYPEWNKLPNLHFYPIPSYNVPMGALIPKDVDRLI
                     VAEKSISVSNLLNGSTRLQPVVLQIGQAAGALAALSVKQKKTISSVSVRDVQSELLNA
                     DCYLLPYLDLPKTDVHFKAVQRIGSTGILKGVGMNVGWSNETWFQCDSLIDVAELKEG
                     LNDFEPAFKWNSKSNELSIKEAIELVASLATFYKIKNADFGTISSNWQKMQLNNYNAE
                     RKITRKEFAVLLDTYIQPFSLKDVDMKGSYIKR"
     gene            complement(5605..6930)
                     /locus_tag="PLZ54_07415"
     CDS             complement(5605..6930)
                     /locus_tag="PLZ54_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ66789.1"
                     /translation="MKKNLLLLLSCCSVLATAQVSFTRVWERSDSAYTETGLEANKKP
                     LYFDDMNTRGIAVGNINSNAGMVERVFLVSRNVLGNVVRVLDVTTGADVKSLDMTNVT
                     GGGFIISDAGVTEDGVLLVSNCTHSSSGPFKIYKWDNEDAAPSVAISYPSGSARYGDK
                     ITVTGKYSDGTARIYAIAGGVLSNYKVQYFGMEEVNGTWQFKQTPDELNTSINAVSSN
                     FHQINPGPDGGYYFRSVGLSNFQQIKSDFTANIGNTPFPTTMPQGGTAPVFIKTVGGF
                     DYACYLQYGSHATYAAPANNRIQVIKIDRLMGITSATVLTSSPSFGKLAPGNGAGHVV
                     VKHQTNGDVDIFALSTQNGVVRYRLSGAEIGTDIRKSVDNLIGIKAFSGKISIEGINP
                     SSIELFNTLGQKIKSISNSNELQTSGLKGIYIVQVKAGGKTIKTEKISL"
BASE COUNT         1970 a         1541 c         1228 g         2304 t
ORIGIN      
        1 cataagctgt acgtttggca acccttgttt cattatcatc cagaatatag tctgttccgg
       61 ctctgaccaa caaatccaaa ttctttgtaa attcatattt tgccgataca tttgccatca
      121 aacgataccg attatctttg ttaatgtttt tatagacaga ccagaatgga ttctgatttt
      181 ttgaaatatc atcggtgtaa cttttagcta atccatccca caaccgcgga aatccgttca
      241 catcttcata gtcacgcata tcgtcgagtt gaatatttcg tggcatacgt aaaaaactat
      301 acatcggatt gtcagggctg tccgctaaat ttggtctgtt ttgtgtattt tgaattaagt
      361 aagttgcacg tccggaaata gtgagttttt taccgagttt tgcatttcca ctcagactga
      421 aactatattt ctttaattcg ctattgggga taattccttt ggagttactt accatcgtcg
      481 ataatcgagc ttgcgcaata tcgctaccgc catccatttc aatagaatgt gtatttcgaa
      541 ttccggtttg aaagaaatct ctaatattgt tgggttgagc tgtaaatgga gcaatatcac
      601 cattccattt ttttaccata cgaccatcca gttttgaatc agcccagttt gttcctttca
      661 acacttcttg ttctactcca tctacaattt cagtttgtcg accgtataga ttttgcaaat
      721 caggcattac atttagcgtt gaaatgccca aatcgtaact atactttaca ccaagtgttt
      781 tttttacaat accttttttg gtggtaatca ttattactcc ggcggccgca cgcgaaccat
      841 aaagtgcagc tgcatttcct cctttcagca tcgaaatgct ttcgatatca tcgggattaa
      901 tgtctgacag accactaccg tagtctatac cgccattttc acttcctgta ccacgtgaat
      961 ctccacttac aggaacaccg tcaacaacaa taagtggctg attattaccg gataaagatg
     1021 ttgcaccacg taataaaata ttggttgttc cccattctcc cgtacctgtt gtgctgactt
     1081 ttaatccagc aacacgtcct gcaagcgaat tcattacatt gtttgattta acctttgtaa
     1141 ggtcgtcgcc tttaacttcc tgaatggcat atccaagcga ttttttttca cgtttgatac
     1201 caagcgcagt tacaacgact tcatccaatg cttttgcatt ttcgttgagg acaactttta
     1261 ttaatccatt acctgtgtgg ctcaatgact gtttttcgta cccgatataa gatacttcaa
     1321 tcatcgaatt tgcatttttt aaagtaagcg aaaattctcc atttacattg gttactgttc
     1381 cattgtttgt tcctttttcc aaaacagttg caccaataat ggcttcacct tttgcgtcgg
     1441 ttacaattcc ttttacaatt tgtgcattta tttgtccgaa tgcgaacaat tgaaatacca
     1501 gtacaattaa aagttttaca ttttttttca tatcattatt ttttatcact cattctaaat
     1561 tcaagctttc ctccgttcat taactgattg aaagaaatgg cttttccgta aaccattttt
     1621 ccgttgattt ttactgattg aatgtatttg tttttttctg aattattctt ggcctcaatg
     1681 ataaatttat ttccgttttc caactgaatt acagcattat gcacaatagg actaccaaat
     1741 acatattctc cattaacggg attcatgggg taaatgccca aagcagacat tacgtaccag
     1801 gccgacattt gtccacaatc gtcgttgccg caaagtccgg ctggtgtggg tttgtacatt
     1861 tcctccagta cctgacgtac gcgttcggct gttttatcgg ctctgtcgac atattgatac
     1921 aggtaaatag tgctgtggct cggttcgttt ccgtgcgcgt actgaccaat caatcctgaa
     1981 atatcaatcg aagcgccttc gtttagtacc gaacttactg taaataaact gtctaactta
     2041 tccgtaaatt tttgtttact accaaaaagt tctatcagtc cttccacatc gtgtggaacc
     2101 aaaaacgtat attgccagcc gtttccttct acataatcgg cttcttcgtg caacgaaaac
     2161 gcaggattgt atcccttgcg gaatgttccg tcagctaatt ttccgcgcat aaatcccaat
     2221 tctttatcga agtaattagc gtaatatttt gccctttttg aaaacaattg ataatcatct
     2281 tcttttccca aatgtttagc cagcaatgca atggcataat catcaatagc atattccagc
     2341 ccttttgaaa ccgaccagtt ttctttatcg gccggaatcc agccctgttc gcgaacaaac
     2401 ttcaatcctc tgtcgtttcg ttgttcaaaa ccgcgaacca aattatatgc acgttcagca
     2461 tcaatgtttt caatattttt caaaaccgca tctacaatca ccggaatgga atgaacccct
     2521 accatcgtac ccgtttcgtt tccaactaaa tgccagattg gcaaactgcc ctgcttttcg
     2581 gtaatatcca gcaaagaatt aacccagtcg gctgttcgct tctgattgat aatagtttgt
     2641 aacggatgaa aagttcggta tgtatcccac aaggagaaaa tggtgtgtgg aacgaaaccg
     2701 tttgctttgt acacttttcc gtcagctccc cgataatcac cattcacatc gcaaaagtca
     2761 gaaggtgaaa tcatggtacg aaacagcgat gtgtaaaaca tggtacgcac tttttcgtcg
     2821 tttgttttaa ttcggatttt cgacaattca gtgttccatt ttgcctgagc ctgagctagt
     2881 gtttccttga aattccagtt tggcaattca gctataaggt tattagccgc atttttgcaa
     2941 cttacatacg atatagccac ttttacttcc accgcttcat tttttttagt ttcaaaactt
     3001 aaaaccgctt tttgcacatc aatactatca ttcgaacgtg gacgaaaaac aggttctacc
     3061 acttttgtaa aattcatgtc ttttgaaaaa acagccgtaa aatatatttt ctggtttttt
     3121 gcccagccgc gcgaataacg ataaccttcg atggtgcgcg catcgatttg tttcaacatg
     3181 gtttcggttg gttcatccca gtcaattcct tcgcccaaat cgataatgat gttcgattct
     3241 acactttcag ggaaagtata acgatgatag cccacacgcg aagtagctgt cagttcggct
     3301 tttatgccgt atttttgcaa aagaacggaa taatatccgg gctgaacttt ttcgttggaa
     3361 tgtgaaaata tagaaacata gcctttcgat aaatcgtcga aagttccttt aaatgttgtg
     3421 ggagttcctg tggttggcat aaacaaaata tcgcccaaat cgccaatacc cgtaccgttc
     3481 aggtgcgtat gtgcgaaacc gatgatcgta ctgtcggaat aatgataccc cgaacaccag
     3541 tgccagcctt tgctcatatt tgtcggtccc aattgcacca tgccaaacgg cacgcttgct
     3601 cccacaaaca cgtttccgtg atagtccgaa ccaatcattg gatcaacata gcgcgtgaaa
     3661 tcaacagcct gcaaatggca ggttatcagc aaaataacag ttaatatttt tgtttttgaa
     3721 atcatctttt tatataactt cctttcatat ccacatcttt caatgaaaac ggttgaatat
     3781 aggtatcgag cagaacagcg aattctttgc gtgttatttt tcgttcggcg ttgtaattat
     3841 tcagttgcat tttttgccag tttgaggaaa ttgttccaaa atctgcattt ttgattttat
     3901 aaaaagttgc caacgatgca actaattcaa ttgcttcttt tattgaaagt tcgttacttt
     3961 tcgaattcca tttgaaagcc ggttcaaaat cgtttagccc ttcttttaac tccgcaacat
     4021 caattaaact gtcgcactgg aaccatgttt cgttgctcca gcccacattc attcccaccc
     4081 ctttcaaaat tccggttgaa ccgatgcgtt gtactgcttt aaagtggaca tcggttttag
     4141 gtaaatccag atagggaagc aaataacaat cagcattcag aagttcgctt tgtacatcgc
     4201 gcaccgatac tgaactgatt gtttttttct gttttaccga aagcgctgcc aatgctcctg
     4261 ctgcctgacc aatttgcaat accactggtt gcaagcgggt tgaaccgtta agtaaatttg
     4321 aaacagaaat cgatttttcg gcaactatca gtcgatcaac atcttttggt atcaaagcgc
     4381 ccatcggtac attgtaggac ggaattggat aaaaatgcag attgggcaac ttgttccact
     4441 ccggataacg gttgtggtga tggtcaatcg gataatcgcc tacagctatg cctgttcggt
     4501 aaagcgcttc gggctgacta aatggtttta caacgtgatt cacgttaaat cttaccaaac
     4561 catgaatacg ccgactctcg cgatgataag gaatcaaagg aaaatgatcg gcagtcggaa
     4621 attgttcttc gtctaatcca taagtggaaa aaccaagttc cgtttgtaaa taatacaagt
     4681 aacaaagcga aaagtttttt gctttttcga gtgcaactgt acgttcggca tcattcattt
     4741 ccagcatgtt aacataaaaa tcatttcctt ctatcggcca gtttatcatg tatttaccat
     4801 taggtaattt accataagtc atcatttttt cttttggcca aagagtttgt ccgggtttag
     4861 gattggtaca gtttgatgat tttgctgtgc aataaaacaa tgatgcatcg taactcgcag
     4921 gctttgtgat ggttttatca aagccataat ctttcagaat cattacataa gtcaggtcct
     4981 gaataatgtc gtttgactgc tcgggtgcaa tatcttctcc acaaactgaa cgtgcttcca
     5041 tcccaatatc gtattttacg ccacaatgtt tggctacatc gcccaactcg gtagcgtcaa
     5101 tcatgatttt tgtagttact gactgtatta caccgccttt actcaccttt actttccatc
     5161 catgtttaat ttttgttgcc gaaatgaact ttgaattttg ccaaacagtt agttttttct
     5221 cagtttttgt cattttttga aaaattgcat ttcccacttt tggttcaaac aaaacattgc
     5281 ttacccagcc tgttttcagg gcatcggcac tgccgtaatg tgccaccaaa ctatctatga
     5341 actcaccaaa aatgcccgaa ggaagttggt ggtttccatc catcgccgac acacctgcag
     5401 aagtaagcat tcctccaagc cagggtgttt cttctacaat aagtgttaga gcgcccattc
     5461 gagcagcctg gatgccggca gcagtaccac ttgtaccgcc gcctacaacc agtacatcca
     5521 ctttttgcgc aacagaagtt aaatgaaata aaacaaaaat tatgaaaact aactttttaa
     5581 gcataaacac gacacatgaa aattctacaa actgatcttt tcggttttta ttgtttttcc
     5641 tccagctttt acctgaacta tataaattcc ttttaaacca cttgtttgca actcgttaga
     5701 gtttgaaatc gattttattt tttggcctag cgtgttgaat aattcgattg aagaaggatt
     5761 gataccttca atgcttatct ttccgctaaa tgcttttatt cctatcaaat tatcaacaga
     5821 tttacgaatg tctgtcccaa tttcggctcc tgaaagacga taacggacaa caccgttctg
     5881 agtcgataaa gcgaaaatat ctacatcgcc attggtttga tgtttcacca caacatgacc
     5941 tgcaccatta cctggagcaa gtttcccaaa tgaaggcgaa gatgttagaa cagttgcact
     6001 ggttattccc attaaacgat caatttttat cacctgaatt ctgttattag caggagcggc
     6061 ataagttgca tgactgccgt attgcaggta acaggcataa tcgaagccac ctaccgtttt
     6121 aataaaaaca ggagcggttc caccttgagg cattgttgtt gggaagggtg tatttccaat
     6181 atttgcagta aaatctgatt taatctgttg aaaattactt aatcctaccg aacggaaata
     6241 ataaccacca tctggtcccg gatttatctg gtgaaaattt gaactgacag cattaatgga
     6301 tgtattcagt tcatcgggcg tttgtttaaa ttgccatgtc ccatttactt cttccatgcc
     6361 aaaatattga actttgtaat tagaaagtac acctccagcg atcgcgtaaa tacgggcagt
     6421 tccatcgcta tattttcccg taactgtaat tttatcgcca taacgagccg aacccgatgg
     6481 ataagaaata gcaacagatg gtgccgcatc ttcattatcc catttgtaaa ttttaaatgg
     6541 gccagaactg ctgtgtgtac aattcgagac taataaaacg ccatcttcgg taactcctgc
     6601 atcgcttata ataaaaccgc cacctgttac attggtcata tctaatgatt ttacatcggc
     6661 tcctgtggtt acatccaaaa cacgaaccac attgccaagt acattacgac ttactaaaaa
     6721 aacacgctca accatacccg cattactgtt aatattgcca acagcaatgc cccttgtatt
     6781 catgtcatca aaatagagag gttttttatt tgcctccagc ccagtctctg tatatgcaga
     6841 atcgctacgt tcccaaactc ttgtaaaaga aacttgggct gttgctaaaa cggagcagca
     6901 actgagcagc aaaagtagat tttttttcat cagaatataa tttattttta atggtctgat
     6961 ggaacccaca tgtctgaatt attatattca ttcgtgtttg tgtatgcaat acatgtaggt
     7021 ttcataaaat tagttttttt aac
//
LOCUS       DAOSCH010000100         6975 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_6965_length_6975_cov_5.891618, whole genome shotgun sequence.
ACCESSION   DAOSCH010000100 DAOSCH010000000
VERSION     DAOSCH010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6965_length_6975_cov_5.891618"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(235..1308)
                     /locus_tag="PLZ54_07420"
     CDS             complement(235..1308)
                     /locus_tag="PLZ54_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ66790.1"
                     /translation="MMASKINPAFQDKLKKYGAVNFNACYNCGTCTAVCSLSTPEESF
                     PREMVRLSSLGLENDIKSSLKPWECYYCGECTENCPQTANPGELMMSLRRWLTSVYDW
                     TGLSGLLYRSVTFTISALIMVFIAVLVLGNAMSFQLAEMMHYGHLFEMYVVFAVLAII
                     FIPNIARMWNFTILRPKVKANLKIYIKSLPDLFVHMFTQKRARDCDKEDNFRWLEHFI
                     LVLAYLSLLFTTVFLDWFGTKSFFVVVLGYVESFFVFVITADFVSSRIRKAKALNKFS
                     QPSDWLFVIWLMLMGLTAFLVRVFIDTHMIDNNVWMYIVHLTVLAQWAIIIVPFGKWS
                     HFLYRSFGMYFSSIKAASEKRKG"
     gene            complement(1305..1586)
                     /locus_tag="PLZ54_07425"
     CDS             complement(1305..1586)
                     /locus_tag="PLZ54_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66791.1"
                     /translation="MMEAEIGKTAKYLRDKQGIPEKAKENLKEFVRIKKALIDALKNG
                     EMTIPQLSEKLSMSKEDVTFYLMTLVKYGDVAVGEIDDNDEYFTYKLNK"
     gene            complement(1591..>1926)
                     /locus_tag="PLZ54_07430"
     CDS             complement(1591..>1926)
                     /locus_tag="PLZ54_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOJ66792.1"
                     /translation="CQGPKNISESVNSALSAATKAFSSISKGLVETEPIVAEINGNSC
                     VWCGECLKACPFEAIEQKEIDGKIIAVINKSVCKGCGMCAPVCVPNAIDLIGYSDKEI
                     FSMIDALVQ"
     assembly_gap    1927..2026
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2027..3141)
                     /locus_tag="PLZ54_07435"
     CDS             complement(<2027..3141)
                     /locus_tag="PLZ54_07435"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ66793.1"
                     /translation="MKKNLGVYICHCGGNISDYVDVEKVRQAIEHEEGVVLAKTTMFA
                     CSDSNQKEMVEDIHQYQLDGVIVASCSPKLHLVTFRNVTETAGLNKYTYVHANIREQA
                     SWAHSDDKEGATEKAIRLVRAAISKSRYAEALYPNKFEAEKAIAVIGAGMAGMKAALS
                     IAEKNMSVVLIEKENTVGGHVAEWGSLFMTNEKGSELVQRIHNDLLKHENISILTGTE
                     VVSSKGSIGNFTLTLQKKHQPVDNLRETMEINVGSVVIATGFDHYIPQRGEFSYLNNE
                     NVISLPDFKKLTDQSTGKTLLYNGKEIHDIAYIYCVGSRQSEGENKYCSRFCCTSTIH
                     TAIETKKKFKGIHNFHLHRGIRTYGKQELLYDEALQNG"
     gene            complement(3150..4448)
                     /locus_tag="PLZ54_07440"
     CDS             complement(3150..4448)
                     /locus_tag="PLZ54_07440"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOJ66794.1"
                     /translation="MEERRKKIEFDALVIGAGIAGGETALNIANNGYKVLLVEKDHSI
                     GGKMILLSKVFPTLDCAACITTPKVSEIARHPNITVFTESEVTSIVKKTESLFIAKIT
                     KNPRYVHVEDCTACQLCEKACPVSVRDEYQFGLVGRKAAYIPFSIANPKAAVIDLEQC
                     TLCGACERVCPTHCIDFTMETECFELQVKTVVLATGFQLFDPLKIPRFGYGKYKNVVT
                     SMQMEREIAPTRPYHTILRPGDGKIPDQIAYVLCTGSRDDSVGNPVCSQVCCMYSMKQ
                     AQLLMGALPMADITIYYIDIRSFGKGFEEFYQQAKDMGVNFVKGKIGKISEKENGNLI
                     LRYEDIQEGIVKEAEHDMVVLSVGMLSNQSVHQIFDGEMLQFDSLNFIWQSDLLGSPA
                     KTNIDGVFVAGTASGPMDIPDSILSAGSASSEAISYLIQH"
     gene            complement(4452..4865)
                     /locus_tag="PLZ54_07445"
     CDS             complement(4452..4865)
                     /locus_tag="PLZ54_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOJ66795.1"
                     /translation="MTSFVKKNAKILVFSTEKISDPAIDMAGLLKLHYPPTVYTITVP
                     CSSGIKPNWILHAFEKGFDGVFIAADGTDCVFGESCTEKTGKVVAMTHEKMKQNNLDP
                     ARLRMAALCSVCSDAFVRQVRSLNEYLIKTRYSEK"
     gene            complement(4877..5128)
                     /locus_tag="PLZ54_07450"
     CDS             complement(4877..5128)
                     /locus_tag="PLZ54_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOJ66796.1"
                     /translation="MTTEELKNLTVAKSVDARGTACPGPLLEAKKAIGTIQSGEILEV
                     LSADEGTKNDLPKWCNKQGNEFLGMVEENGYYKLYLKKK"
     gene            complement(5167..5736)
                     /locus_tag="PLZ54_07455"
     CDS             complement(5167..5736)
                     /locus_tag="PLZ54_07455"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOJ66797.1"
                     /translation="MEKSNEITMEKLLAELENLKAKTTQLENSAKKDQLSIAIISGDM
                     DKIMAGMIISLAAAAMDTKVKLFFSFWGLAALRDPKKTVKGKNFVSKMFGCMLPKGYG
                     KLKLSKHNMCGMGPMMMKNLMIKQHVLSLDEMFKEAAELGIEITACEMTMNLMGFKKE
                     EFIDYPHFRIAGAATFVADAGESAMQWMI"
     gene            complement(5935..6657)
                     /gene="recO"
                     /locus_tag="PLZ54_07460"
     CDS             complement(5935..6657)
                     /gene="recO"
                     /locus_tag="PLZ54_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOJ66798.1"
                     /translation="MQVKTEGIVFHSLKYSDNSAIVTIFTRRFGRVAYMIYGRGKRKS
                     SWKPAFFQPLSIVELEVSHDMRKNIQQMKDVRVLVPYQSLTIDPLKRSIAMFIAEVLL
                     KVLSQSEADESLYLFLRQSLEFLDNSKQKTANFHLVFLLKLSRYLGFEPNIDGQTTLF
                     FDLVNGVFLSSPPAHRHYLFAEEAGKMAQLLQTDYEHMTEIKLSRQQREKLLEDIVSY
                     YRFHLPDFPEIHSLEVLKTLFD"
BASE COUNT         2074 a         1446 c         1194 g         2161 t
ORIGIN      
        1 tatgagacaa agataataat ttatttgtgc aataatttaa tgtaatgtat ataagtctat
       61 aggccatatt ttttatgttt tgcaaaaaga aaaaatattt tccaaatgtc aataaattcg
      121 gtaaaagatt gaataacatt gatggataat gatttgaact tcacgacaga aatgttgaaa
      181 gctgtctttc ttttctaaaa aaatctattt gtatttttct acagaaacga ttctttaacc
      241 ttttcgtttt tctgaagctg ctttgatgct tgaaaaatac atgccaaaag accgatacag
      301 gaaatgagac cattttccaa atggaacaat aattatagcc cattgagcca aaacggttaa
      361 atgaacgata tacatccata cattattgtc tatcatatgg gtatcgataa atacgcgtac
      421 caggaaagct gtaagcccca taagcataag ccatataaca aacagccagt cggaaggctg
      481 cgaaaatttg ttcagtgcct ttgctttcct aattcgactt gaaacaaaat cggcagtaat
      541 gacaaataca aaaaaacttt ccacataacc caacactact acaaagaaac tttttgtgcc
      601 aaaccaatct aaaaatacgg ttgtgaaaag cagcgaaaga taagccaaaa ccagaataaa
      661 atgctccagc caccgaaaat tatcttcttt atcacaatct cgggcgcgtt tttgagtgaa
      721 catgtgaaca aacagatccg gtaatgattt tatgtaaatt tttagatttg cttttacttt
      781 tggtcttaaa atggtaaaat tccacattct ggctatattg gggataaaaa ttattgcgag
      841 cactgcaaaa accacataca tttcaaaaag atgtccataa tgcatcattt ctgccaattg
      901 aaatgacatg gcattaccca aaacaagtac ggcaataaat accattatca gagcagaaat
      961 agtgaatgta acggaacgat aaagcaaacc cgaaagtccc gtccagtcat aaactgaagt
     1021 taaccatcgt cgcaaggaca tcatgagttc tcccggattg gctgtttgag gacaattttc
     1081 ggtacattca ccacaataat agcattccca tggttttaaa gaagatttaa tatcattttc
     1141 aagtccaaga ctgctaagtc ttaccatttc gcgaggaaaa gattcttcag gtgtggacag
     1201 tgaacataca gcagtacagg taccgcaatt gtaacaggcg ttgaaattta ctgctccgta
     1261 tttcttcaat ttatcctgaa atgcaggatt aatttttgat gccatcattt gttcaattta
     1321 taggtaaaat attcgtcatt gtcatcaatt tctcccactg caacgtcgcc atatttaacc
     1381 agcgtcatca aataaaatgt aacatcctct tttgacatgg atagtttttc cgatagttgg
     1441 ggaatggtca tttctccatt tttcagggca tcaatgagtg cttttttaat cctgacaaat
     1501 tctttaagat tttcttttgc cttttcggga ataccttgtt tgtcccttaa atatttggcc
     1561 gtttttccta tttctgcttc catcattttt ttattgaacc agtgcatcta tcatactaaa
     1621 aatctcctta tcggaatagc caatcaaatc aatagcattg ggtacgcata caggagcgca
     1681 cataccgcaa cccttgcata ccgatttgtt gattacagca attattttcc catcaatttc
     1741 tttttgttca attgcttcaa aaggacaagc ttttaaacat tcaccgcacc aaacacatga
     1801 attaccgtta atttcagcaa ctatcggctc cgtttctacc aatcccttac tgatggagga
     1861 aaaggccttt gtggcagcag acaaggccga attgaccgat tcgctgatat tttttggccc
     1921 ttggcannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccat tctgtaaagc
     2041 ttcgtcatat agtaactcct gtttgccgta agtgcgaata ccccgatgaa gatgaaaatt
     2101 gtgtatcccc ttaaatttct tttttgtttc gatagcagta tggattgtcg aagtacagca
     2161 gaaacgcgaa cagtatttat tctcaccttc ggattgacga cttcccacgc aatagatata
     2221 agcaatgtcg tgaatttctt ttccgttgta taaaagtgtt ttacctgtcg attgatctgt
     2281 cagtttttta aaatcgggca aagaaattac attctcgtta ttgagatagg aaaattcgcc
     2341 tcgttgcgga atataatgat caaaacctgt agcaatcact acagaaccta cgttaatttc
     2401 catagtttct ctaagattgt caacaggctg atgttttttt tgaagagtca gcgtgaaatt
     2461 tccaatgctg ccttttgaag aaacgacttc ggtacctgtg agaatagaaa tgttttcgtg
     2521 tttcagcaaa tcgttatgaa tacgttgaac aagctccgaa cccttttcat ttgtcatgaa
     2581 aagcgatccc cattctgcta catgccctcc aacagtattt tctttttcaa tcagcactac
     2641 agacatattt ttttcggcaa tggaaagagc tgctttcatt cccgccattc ctgctccgat
     2701 tactgcaatt gctttctctg cttcaaactt attggggtac aaagcttctg cgtaacgcga
     2761 ttttgaaatg gctgccctta ccagtcgaat cgctttttcg gtagctcctt ctttatcgtc
     2821 cgagtgtgcc caagaagcct gctcacgaat attggcatga acgtaggtat atttgttcaa
     2881 accggctgtt tcagttacat ttctaaaagt aaccaaatgc agttttggag agcaggatgc
     2941 cactatgact ccatccaact gatattgatg aatatcttcc accatttcct tctgattgct
     3001 atccgaacat gcaaacatgg tggttttcgc taaaacgaca ccttcttcat gttcaattgc
     3061 ctgacgtact ttttctacgt cgacataatc tgaaatattt ccaccacaat gacagatata
     3121 aacgcctaaa ttctttttca tcaatcttat taatgttgaa ttaagtaact gattgcttcg
     3181 gacgaagccg aaccggcaga tagaatggaa tcgggaatat ccattggacc agatgccgtg
     3241 ccggcaacga aaactccatc tatatttgtt tttgcaggac ttcccaacaa gtcgctctgc
     3301 caaatgaaat tcaaagaatc aaattgcaac atttcgccat caaaaatttg atgaactgac
     3361 tgattggaca acattcctac cgataatacc accatatcgt gttcggcttc tttcactatt
     3421 ccttcctgaa tatcttcata acgcagtatt aaattcccat tttctttttc ggaaattttt
     3481 ccaatttttc cttttacaaa attgacaccc atatctttgg cttgctggta gaattcttca
     3541 aagcctttgc cgaaagaacg aatatcaatg taataaatgg taatgtctgc cattggaagc
     3601 gcacccatta acaattgtgc ctgcttcatg gaatacatgc aacatacttg tgagcaaaca
     3661 ggattaccaa cgctgtcgtc gcgcgagccg gtacaaagaa cataagcaat ctgatcgggt
     3721 atttttccgt cgcccggtct cagaatagtg tgataaggtc tggtaggagc tatttctctt
     3781 tccatttgca tggaagtaac cacatttttg tattttccat agccgaatcg tggaatttta
     3841 agcggatcaa acagttgaaa acctgtagcc agtacgactg tctttacctg taattcaaaa
     3901 cattccgttt ccatagtaaa gtcgatgcag tgagtagggc aaaccctttc gcatgcgccg
     3961 cacaaagtac attgttcaag gtcgataaca gcagctttgg gattggctat gctgaaagga
     4021 atgtaggcag cttttcgacc aaccagacca aactgatatt catcccgaac actaacggga
     4081 caagcttttt cacacaattg gcaagccgta cagtcttcca catgaacgta acgcggattc
     4141 tttgtaatct ttgctataaa taacgattcg gtttttttta caatacttgt aacttcactt
     4201 tcagtaaaaa ctgtaatatt gggatgacgg gcaatttcag aaactttggg tgtggtaata
     4261 caggcagcac aatccaatgt tggaaaaact ttgctcaaca aaatcatttt accaccaata
     4321 gaatgatctt tttctaccaa tagcacctta taaccgttgt ttgcaatatt cagggcagtt
     4381 tcgccgccgg caatacccgc accaataacc agcgcatcga attctatttt ttttcttctc
     4441 tcctccattg tttatttctc cgaataacgg gttttaatca aatattcatt cagactgcgc
     4501 acttgcctga caaaagcatc gctacaaacg gagcaaagag cagccattct caagcgggcg
     4561 ggatccaaat tgttctgttt cattttttcg tgggtcattg ctactacttt accggttttt
     4621 tcggtgcagc tttcgccaaa aacgcaatcg gtaccatcag cagcaatgaa aacgccatcg
     4681 aagccttttt caaaagcatg caggatccaa ttgggcttta ttccgcttga acatggaaca
     4741 gttatggtat aaacggttgg cggataatgc agtttaagca gcccggccat atcaatagcc
     4801 ggatctgaaa ttttctcggt agaaaatacc agaatttttg catttttctt tacgaagctc
     4861 gtcatggaga aaaaaattat ttctttttca gatacaattt ataatatccg ttttcctcta
     4921 ccatgccaag aaactcgttt ccttgcttgt tgcaccattt tggcaaatca ttctttgtgc
     4981 cttcatcggc cgacaaaact tccaaaattt ctcccgattg tatggttcca attgcttttt
     5041 tagcttccaa aagtgggcca gggcaggcag ttccgcgtgc atctaccgat ttggctacgg
     5101 taagattttt caattcttcg gtggtcataa ttctataatt tttaatgttt gtttatgggt
     5161 tattacttat atcatccatt gcatggcgct ttcgccggca tctgccacaa aagttgctgc
     5221 tcctgcaatt cggaaatgtg gataatcgat aaattcttct tttttgaagc ccatcagatt
     5281 catggtcatt tcgcaagccg taatttctat tcccaattcg gcggcttcct taaacatttc
     5341 gtccaatgaa agtacatgct gttttatcat caaatttttc atcatcatgg gtcccatacc
     5401 acacatattg tgtttggaca atttcagttt cccatatcct ttgggcaaca tgcatccgaa
     5461 cattttggaa acaaagtttt tccctttcac agttttcttg ggatcacgta atgctgccaa
     5521 tccccagaat gagaagaaca gttttacctt tgtatccata gctgccgccg ccagcgatat
     5581 aatcattcct gccataattt tgtccatgtc gcctgagata attgctatcg acaattggtc
     5641 ttttttggct gaattttcca attgcgttgt tttggctttc aagttttcca gttcagccaa
     5701 aagtttttcc atggttattt catttgattt ttccataata atagtatttt taaattgatt
     5761 tctgcacaaa ttaacggaaa aatttatgaa tctgctatat cttacaggaa tatttattcg
     5821 gaaaaattca aaataaatcg gataaacgac ttttgaagaa ggttgtcgga aatagctttt
     5881 ttgtatccgc aaaaaaaatt aaaaagtcaa taattcccat attgacaaac gccattaatc
     5941 aaaaagagtt ttcagcacct ccagcgaatg aatctcggga aaatcgggaa gatgaaaacg
     6001 ataataactc actatatcct ccaataattt ttctcgctgc tgacgtgaaa gtttgatttc
     6061 ggtcatgtgc tcatagtcgg tttgcaacaa ctgagccatt ttcccggctt cttcggcaaa
     6121 gagataatgc cgatgagcag gcggagagga caaaaaaaca ccgtttacca aatcgaaaaa
     6181 aagtgtcgtc tgtccatcga tattgggttc aaagcccaag tatcgcgaaa gtttaagtaa
     6241 aaataccaga tgaaaattcg ccgttttttg cttactgtta tccaaaaact ccaatgattg
     6301 ccgcagaaaa aggtagagtg attcatcggc ttcgctttga gaaagcactt tcaaaagcac
     6361 ttcggcgata aacatggcaa tagaacgctt gagaggatcg atggtcaggc tctggtaagg
     6421 cacaagcacc cttacatcct tcatttgctg aatattcttt cgcatatcgt gcgaaacttc
     6481 cagctctact atcgacaaag gttgaaaaaa agcaggtttc cacgaagact ttcttttccc
     6541 tctgccataa atcatgtaag caactcgtcc aaaacgacgg gtaaaaatgg ttactatcgc
     6601 agaattgtcc gagtacttta gtgaatgaaa aactatacct tcggttttga cttgcacagc
     6661 tcgctccttt ttttgaaaac aaaattacgg gaaaaatctc gttttttacc tgattcgatg
     6721 aagaaaccaa tgcaaaactt aatgctaaat tctcactttt aagttgaact tagcatccgt
     6781 ccttccccaa aaacaaaaaa aattcgtttc aaaacgacat ttaaaatcaa gcaatacccc
     6841 atatcgctga aaaattaaaa aatttaaaac aaagtctttg atatttcaaa aatctgccgt
     6901 atatttgcac ccgcaattgt gcaaaacagt tgaagttttt cataacattt ttggcccatt
     6961 cgtctatcgg ttagg
//
LOCUS       DAOSCH010000101         6950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_6995_length_6950_cov_9.837128, whole genome shotgun sequence.
ACCESSION   DAOSCH010000101 DAOSCH010000000
VERSION     DAOSCH010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6995_length_6950_cov_9.837128"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(144..1307)
                     /locus_tag="PLZ54_07465"
     CDS             complement(144..1307)
                     /locus_tag="PLZ54_07465"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HOJ66799.1"
                     /translation="MQYFKELAICISVLLCFSCQKNNEIAAKSPSLSIRWDESSLVCI
                     AAEGGYPRVKRLNDGSLLAAYENRKGDVLVKQSRDEGKTWSDSVLVFGSFDYIHPSNG
                     LSAKINIANPEFIQLGNDDILFACNFRPNKDEVYPFSIAVKRSRDNGKSWSEQQIVYQ
                     AAPRFIDGCWEPSFLLLPDGRIQLYFANEFPYQQSNEQEISMLESNDNGTSWSADKKT
                     VSFRKGFRDGMPVATTDGNEIFVAIEDNGFDQFKPFIVKNAIADNWKEPVLEESPNRY
                     SALKEPLPSSVYAGAPYLIRTKEGYYVLSYQTTHHRTSNWELSTMEVVVSDKPSDFIS
                     PSQPFAVPLSKEAKWNSLCDLGDGEIAAVSSTNFKLDKIGIWMIKGKIVDNRK"
     gene            complement(1286..3097)
                     /locus_tag="PLZ54_07470"
     CDS             complement(1286..3097)
                     /locus_tag="PLZ54_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008998196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ66800.1"
                     /translation="MLRVKLLLLSSLCSVITFAQWQPSGERIKTRWASQIDVNNVLPE
                     YPRPLMERSEWMNLNGLWNYTILPVGKQAPSAFDGKILVPFAVESSLSGVQKNVGKAN
                     ELWYEREFMIPAKWKNNRVILHFGAVDWKTDVWVNNVKVGQHTGGYTPFSFEITAALK
                     NGSNKLMVKVWDPTNDGYQPRGKQVNKPEGIWYTPVTGIWQTVWLEPVPDKFITQVKT
                     TPNIDINILNVEVATNNFSASDRIEVKVMDGSKVVATAQSINHLPVEIVMPDQVKLWS
                     PDSPFLYDLEITLWNGTKQIDKVKSYAAMRKYSIKRDENGIVRLQLNNKNLFQFGPLD
                     QGWWPDGLYTAPTDEALKFDIQKTKDFGFNMIRKHVKVEPARWYTYCDRLGIIVWQDM
                     PSGDGGPQWQPHQYFNGLEWLRSPESEANYRKEWKEIIDFLYSNPCVGVWVPFNEAWG
                     QFKTIEIAEWTKQYDPSRLVNPASGGNHYPVGDMLDLHNYPNPALYLYDAQRTTVLGE
                     YGGIGWAVEEHLWEPDRNWGYVQFNSAKEVTDEYVKYAEQLMNLIQHGFSAAVYTQTT
                     DVEVEVNGLMTYDREIIKMDESRVKQINQKICNILKN"
     gene            complement(3207..>6950)
                     /locus_tag="PLZ54_07475"
     CDS             complement(3207..>6950)
                     /locus_tag="PLZ54_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOJ66801.1"
                     /translation="CILQDKDEKLWLGTDEGVYIYEPRSEKFQRFVVKTTTNDSVRGV
                     VSDMIIDSEGDIWISVEEKGVYLYRYGSNTLDHFPVKTPLGGLKKIKLCENKGKGVWA
                     FPYSMPFLQIDKKSRKIQPLFSLNQGNEILYNTGEIGSVIEEQNNLLVGTSLQGLLQI
                     NLVERTYNKLLASDETGQPIFVRNILRKGDDLWVATESGIYIYNLYSREVINLRHNNV
                     IPYSLSDNAVYSLYEDNEGGVWMGTYFGGVNYFPNRLTYFEIFYPLENENSIKGRRVR
                     EFCENMDGRLWIGTEDNGLNLFDPETSQFLPIDSKLNSLYHNIHALFRDGNILWIGTF
                     SKGLNCYNLKTKTLKTYYKTSQSNSLSENSVFSICKDRNNTLWIGTLSGLNTYDYKED
                     SFTRIKKLEGAFVQNIFEDSEGNIWVATFVKGLYKYDPSIKIWKNYVHTSASGSLPYN
                     KITSVYEDSKKSIWITTEGGGFCQYDKKNDTFITWNSSNGLPNDVVYQIKEDNFGNLW
                     LTTNAGLVQFDPEKKSFKNFTVEDGLKTNQFNYKSSYKAPDGTLYFGSIDGFIRFNPA
                     YFKENNILPPIVLTDFYLNNLPANVSDKGSPLKESISYAQKIQLPYNKNSFTIHYAIL
                     NYSGLRPKNVVYKLEGFDKQWIPANGSQSVVYSNLNPGKYRLIISFDTNSSGGVNKVS
                     KTLDIIIKPPFWETWWAYLLYFLIIITGIFELINYFRQRTKRIHLEKMRDFEQEKEKE
                     LYESKIDFFTNVAHEIRTPLSLIKAPLDQILRNDETVIADVKDNLKVMSKNTDRLMDL
                     TNQLLDFRKTEAELYKLKEKRINLSELMKETFDRFTPLAKQKNISFSLKLPEKEIVTK
                     IDAEAFTKIISNLINNAVKYCNGMVNISLEALPENHPEKLIFKTENDGAVIPEKFKNE
                     IFKPFTRLNKEKDKIITGTGIGLTLTKSLIELLKGTISYQVENNLNVFKITLPIKLLS
                     EKETIENKRESDEILEKLDKEKIQTSKKQPSLLLVEDDKELKDFLVKCLKNNYNVFIS
                     ANGKEALEILKEKNIDLVISDVMMPEMDGFELTSFIKSHIEYSHIPVILLTAKVNVQS
                     KVQGFEVGADAYIEKPFSVEVLLAQVASILQNREKFREIFLKNPFKGFNNAQLNKSDE
                     EFLQKLEDIIQENIANPDFNIEDLAEQFFMSRASFYRKIKGILDLTPNEYLRVARLKK
                     AAQLLKEGNYRVTEICYMVGFNSPSYFAKCFQQQFGVLPKDFS"
BASE COUNT         2116 a         1403 c         1139 g         2292 t
ORIGIN      
        1 cgttgggtca ggcaaacttc gtggaatgat gggatttttg taagtgctaa cactctctgt
       61 ttcgggaatg attgcctcac ttcccttaca cgaaatgaaa aacagtgtca ataatattat
      121 tccaagcgaa cgtttcataa attttatttt cgattatcta caatttttcc tttaatcatc
      181 caaataccga ttttatccaa tttgaaattg gttgaagaaa cagcagcaat ttcgccatct
      241 cccaaatcgc acagcgaatt ccatttggct tccttgctta aaggtacagc gaaaggttgc
      301 gatggtgaaa taaaatcgga cggtttatcg ctgacaacaa cttccattgt ggacaattcc
      361 cagttggaag ttcgatgatg ggtcgtttga taggacaaaa cgtaatatcc ttcttttgtg
      421 cgaatcaagt aaggtgcacc cgcataaact gaagaaggaa gaggttcttt caaagctgaa
      481 tagcgattgg gggattcttc taaaacaggt tctttccaat tatcagcaat ggcatttttc
      541 acaataaaag gtttgaattg atcaaagcca ttgtcttcga tggctacgaa aatttcattt
      601 ccatcggtcg ttgcaaccgg catgccatca cgaaatccct tgcggaagct cactgtcttt
      661 ttatcggccg accagcttgt cccattatca ttggattcga gcatggaaat ttcctgttcg
      721 ttgctttgct gatagggaaa ttcattggca aaatagagtt gaattctgcc atccggcaaa
      781 agaagaaaag aaggttccca acatccgtca atgaatcgcg gagctgcctg ataaacaatt
      841 tgctgttcgc tccacgattt tccgttatca cgacttcttt ttacggcaat agaaaaaggg
      901 taaacttcat ccttattggg acgaaagttg caggcaaaca gaatatcgtc attcccaagc
      961 tggataaatt ccggattggc gatattgatt tttgccgaaa gtccatttga aggatgaata
     1021 taatcgaaac ttccaaaaac caacaccgaa tcgctccatg ttttgccttc atcccgactt
     1081 tgttttacca acacatcccc ttttctgttt tcgtaggcag ccaaaagcga accgtcgttc
     1141 aatcttttta cacgcggata tccgccttcg gcagcgatac acaccaacga actttcatcc
     1201 catcgaatag acagtgaagg tgatttcgct gcaatttcgt tgtttttctg acacgaaaaa
     1261 caaagtaaaa cagatataca aatggctaat tctttaaaat attgcatatt ttttgattta
     1321 tctgtttgac tctcgattca tccattttta ttatttcacg atcgtaagtc atcaaaccat
     1381 tcacttcaac ttctacatcg gtagtctgtg tataaacggc tgcggaaaaa ccatgctgaa
     1441 tcaaattcat cagttgttcg gcatatttca catactcatc cgttacttct ttggcactgt
     1501 taaactgaac atatccccaa ttgcggtctg gttcccacaa atgttcttcc acagcccaac
     1561 cgattccacc atattcgcct aaaactgtag tgcgttgtgc atcatacaga tacaatgcag
     1621 gattaggata attgtgaaga tccaacatat cgccaactgg ataatgattg ccaccactgg
     1681 caggattaac taagcgagaa ggatcgtatt gttttgtcca ttctgcaatt tctatcgttt
     1741 taaattgacc ccatgcttca ttgaaaggaa cccaaacacc tacacatgga ttggaataca
     1801 gaaaatcaat tatttctttc cattccttac gatagtttgc ttcggattcg ggcgaacgaa
     1861 gccattccaa gccattgaaa tactgatggg gctgccattg cggtccacca tcaccacttg
     1921 gcatatcttg ccaaacaatt atgcccagac ggtcgcaata agtgtaccag cgtgcgggct
     1981 cgactttgac atgtttgcga atcatgttaa aaccaaaatc tttggttttc tgaatatcaa
     2041 attttaaagc ttcatcagtt ggagcagtat acaaaccatc aggccaccaa ccctgatcca
     2101 gcggaccaaa ttggaacaaa ttcttgttgt tcagctgtaa acgaacaatt ccattctcat
     2161 ctcgcttaat ggaatatttt cgcatagcag cataactttt aactttgtcg atttgttttg
     2221 ttccattcca aagcgtgatt tccaaatcgt acaaaaaagg actgtcgggc gaccaaagtt
     2281 taacttgatc aggcataaca atttctacgg gaagatgatt gatggactga gccgtagcca
     2341 caacttttga gccatccata acttttactt caattctgtc cgaagcagaa aaattgtttg
     2401 ttgcaacttc aacattcaaa atatttatat caatattcgg tgttgttttt acctgagtaa
     2461 taaatttatc cggaacgggt tcgagccaaa ctgtttgcca aatgccggtt actggcgtgt
     2521 accaaatgcc ttcgggttta ttcacctgct tcccacgtgg ttgataacca tcattggttg
     2581 gatcccaaac cttaaccatc aatttgtttg aaccgttttt tagtgcagca gtaatttcaa
     2641 atgaaaaagg tgtatatcca cctgtatgct ggccaacctt aacattgttc acccaaacat
     2701 cggttttcca atccacagcg ccaaaatgca gaattacccg attatttttc cattttgcag
     2761 gtatcataaa ttcacgttcg taccataatt cgtttgcttt tccgacattc ttctgaacgc
     2821 cagacaaact cgattcaaca gcaaatggaa ccagaatttt cccatcaaaa gcagaagggg
     2881 cttgttttcc aaccggaaga atggtataat tccataaacc attgagattc atccattctg
     2941 aacgttccat caaaggccga ggatactcag gcaatacatt attgacatca atttgagacg
     3001 cccagcgcgt tttgattctt tctcctgatg gttgccattg ggcaaaagta atgacactac
     3061 acagtgagct taacaataat aattttactc ttaacattgg ctatattttt ttaaaattct
     3121 ttatcgagca aacttagtta aaaaatcaat aatcaaaaga aaaaaatgaa tcgaaaaata
     3181 acaatattat ctcaaaatag cagaatttac gaaaaatctt tgggcaaaac gccaaattgt
     3241 tgttgaaaac atttggcaaa ataagaagga gaattgaatc cgaccatata gcaaatttct
     3301 gtaactcggt aatttccttc ctttaagagt tgagctgctt ttttgagcct tgccacccga
     3361 aggtattcat tgggcgtcaa atcgaggatt ccttttattt ttctgtaaaa gctggcacgg
     3421 ctcataaaaa attgttcagc taaatcctca atattgaaat caggattggc aatattttct
     3481 tgaattatat cttctaattt ttgaagaaat tcctcatcag atttattaag ttgagcatta
     3541 ttaaaaccct tgaatggatt tttcaaaaaa atttcacgaa atttctctct gttttgaaga
     3601 atacttgcaa cttgagccaa taaaacctcc acagaaaaag gcttttcaat ataagcatca
     3661 gcgcctactt caaaaccctg caccttagac tgaacattaa cttttgccgt taaaagaatg
     3721 accggaatat gactgtactc gatatgcgat ttgataaagg acgtcagttc aaacccatcc
     3781 atttcaggca tcataacatc gctaattacc aaatctatat ttttctcttt caaaatctcc
     3841 aatgcttctt ttccattggc agaaataaat acattataat tatttttcaa acattttact
     3901 aaaaaatcct ttaactcttt atcatcttct actaagagaa gggaaggttg ttttttggat
     3961 gtttgtattt tttccttatc aagcttttcc aaaatttcat ctgattctct tttattttcg
     4021 atagtttctt tttctgatag aagctttatt ggtaaagtaa ttttgaaaac attcaaatta
     4081 ttctctacct gatagcttat tgttcctttt aataactcaa ttaatgattt cgttaaagtt
     4141 aatcctatac ccgtccccgt tataatttta tctttttctt tatttaaccg agtaaaaggc
     4201 ttaaatattt catttttaaa tttttcagga ataactgctc cgtcattttc cgttttaaaa
     4261 ataagttttt cgggatgatt ctcaggcaat gcttcaagcg aaatattcac catgccgttg
     4321 caatatttca cagcattgtt gataagatta ctaatgattt ttgtgaaagc ttctgcatca
     4381 atttttgtaa cgatttcctt ttcagggagt tttaagctaa aggaaatatt tttctgcttt
     4441 gccagtggtg tgaagcgatc aaaggtttct ttcatcaact cagacaaatt gatgcgtttc
     4501 tctttcaatt tgtacagttc agcttctgtt tttctgaaat ccagcaactg attggtcaaa
     4561 tccatgagtc gatcggtatt tttgctcatc accttcaaat tgtctttcac atcagcaata
     4621 acggtttcat catttcgtaa aatttgatcc aatggagctt ttattaaaga cagtggcgtt
     4681 ctgatttcat gggctacatt ggtgaaaaaa tctatttttg attcatagag ttctttttcc
     4741 ttttcctgct cgaaatcacg cattttttcc aaatgaattc tcttggttct ctggcgaaaa
     4801 taattgatca attcgaaaat tccggtaata attatgagaa aatataacag ataagcccac
     4861 catgtttccc aaaaaggtgg ttttataata atatccaatg ttttactgac tttattcaca
     4921 cctcctgagg aatttgtatc gaaagagata atcaatcgat attttccggg attcaaattg
     4981 gaatatacaa ctgattgaga accgttggcc ggaatccatt gcttgtcgaa tccttccagt
     5041 ttatatacta catttttagg acgtaaaccg gaataattca aaatagcata atgaatggta
     5101 aaagaatttt tattgtaggg aagttgaatt ttttgtgcat acgaaatact ttcctttaat
     5161 ggagaacctt tatcggaaac atttgctggc aaattattca ggtaaaaatc ggtcaaaaca
     5221 ataggaggca aaatattgtt ttccttaaaa taagccgggt tgaaccgtat aaaaccatca
     5281 atggagccaa agtaaagcgt tccgtcagga gctttataac tggatttata attaaattga
     5341 ttggttttca agccatcttc taccgtaaaa tttttaaatg attttttttc cggatcaaat
     5401 tgaacgagtc cagcattggt tgttagccat aaatttccaa aattatcttc ttttatctgg
     5461 taaacaacat cattgggcaa gccattcgaa ctgttccagg taataaacgt gtcatttttt
     5521 ttatcgtact ggcaaaatcc tccaccttct gtagtgatcc agatactttt cttgctgtct
     5581 tcataaacag aagtaatctt attataaggt aaacttcccg atgctgaagt atgaacataa
     5641 tttttccata tttttatgga aggatcgtat ttgtacaatc cttttacaaa agtagctacc
     5701 caaatatttc cctcactatc ttcaaaaata ttctgtacaa aagctccttc caatttttta
     5761 attcttgtaa aagaatcttc cttataatca taagtattta atcctgataa agttccaatc
     5821 cataacgtgt tgtttctatc cttgcaaatg gaaaaaacgc tgttttctga caaggaatta
     5881 gattgactag ttttataata ggtttttaaa gttttggttt tcaaattgta gcaattcagc
     5941 cctttagaga aagttcctat ccaaaggatg ttgccatctc taaaaagggc atggatattg
     6001 tggtataagg agtttaattt actatcgata ggaagaaatt gtgaagtttc aggatcgaac
     6061 aaattcaatc cattgtcctc tgttccaatc cacaatcgtc catccatatt ttcgcaaaat
     6121 tctctaaccc ttcttccctt tatcgaattt tcattttcaa gaggataaaa aatttcgaaa
     6181 taagtaagtc ggttcggaaa ataatttact ccgccaaaat aagttcccat ccatacgccg
     6241 ccttcattat cttcataaag ggaataaaca gcattgtccg aaagcgaata gggaataaca
     6301 ttattgtgcc ttaaattaat gacttcacgg gaataaagat tgtaaatata aattcccgat
     6361 tccgttgcca cccacaaatc atcacctttt cgtaagatat ttcgcacaaa aatcggctgt
     6421 ccggtttcgt ccgaagccaa taatttgttg taagttcgtt ctaccagatt aatttgtaaa
     6481 agtccctgca aggaagtacc taccagcaaa ttgttttgtt cttcaattac gcttccgatt
     6541 tcacccgtat tataaaggat ttcattgccc tgattcaaag aaaacaacgg ttggatctta
     6601 cgactttttt tgtcaatttg cagaaacggc attgaatatg gaaaagccca aactcctttt
     6661 cccttatttt cgcagagttt aatttttttc aatcctccga gaggcgtttt aaccgggaaa
     6721 tgatccagtg tattgctccc ataacgatac aaataaactc ctttttcttc gaccgaaatc
     6781 caaatatcgc cttcactgtc gataatcata tcgctgacga cacctcttac tgaatcgttt
     6841 gtggtcgttt tcactacaaa acgctgaaac ttttcacttc taggttcata aatgtaaacg
     6901 ccttcatcgg ttcccaacca taatttttcg tccttatcct gcaaaataca
//
LOCUS       DAOSCH010000102         6801 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_7192_length_6801_cov_6.466795, whole genome shotgun sequence.
ACCESSION   DAOSCH010000102 DAOSCH010000000
VERSION     DAOSCH010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7192_length_6801_cov_6.466795"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..2296
                     /gene="pnp"
                     /locus_tag="PLZ54_07480"
     CDS             158..2296
                     /gene="pnp"
                     /locus_tag="PLZ54_07480"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOJ66802.1"
                     /translation="MTVVTKTITMSDGREIVIETGKLAKQADGSVMVRMGDTMLLATV
                     CSAQDANEGTDFMPLSVDYKEKFAAFGRFPGGFLRREGRPSDYEILISRLVDRALRPL
                     FPDDYHAETFVNIMLCSADGENIPDALAGLAASAALAVSDIPFNGPVSEVRVARIDGN
                     FVVNPTFNQLENADMDIIVAATMDNIMMVEGEMKEVSEDDLLDAMKVAHEAIKLHCKA
                     QLELMETVGKTKKREYCHEQNDEELKKDIWEKTYDKVYAEAASGNADKHSRSEHFEAI
                     MNEYIASLDTETAEAKKPLIERYYHAIEKEAMRRAILDEGKRLDGRKTTEIRPIWSEV
                     DYLPGTHGSSIFTRGETQALATVTLGTKMDEKLIDEPLIQGKERFLLHYNFPPFATGE
                     ARASRGIGRREIGHGNLAMRALKNVIPDDYPYVIRVVSDILESNGSSSMATVCAGTLA
                     LMDAGVQIKKPVAGIAMGLISENQGKNYAVLSDILGDEDHLGDMDFKVTGTREGITAT
                     QMDIKVDGLSYEILEKALRQAKEGRLHILNKMLETISEPRADFKPHVPRIVVMFVPKE
                     MIGAIIGPGGKIIQNIQLETGATITIEEIGDQGRVEIAATNKDSIEAAINKIKGIVAI
                     PEVGETYIGKVKSIMSYGAFVEFLPGKEGLLHISEIEWKRTESMDQTGIKEGDMIEVK
                     LLDIDEKTGKFKLSRKALLPRPPKEEKPRN"
     gene            complement(2476..3117)
                     /locus_tag="PLZ54_07485"
     CDS             complement(2476..3117)
                     /locus_tag="PLZ54_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66803.1"
                     /translation="MFLNIPLLEWIGYLASIIVAISLTMSSIKKLRWLNLIGSAVFSF
                     YGFSIGALPVGFLNLFIVLVNIYYLIRMNSAQESFKALAVEPNDAYFNYFIEFHKKEI
                     LQIFPHFELASTKQDKTSSIALLLLRNAVAAGVFYGTIDNGCLYVELDFVASEYRDLK
                     PGEFIYKTNVQQLKETGIHKLVAKSEHPQHHKYLIKMGFVRRNDTPAVFEKEI"
     gene            complement(3287..4732)
                     /locus_tag="PLZ54_07490"
     CDS             complement(3287..4732)
                     /locus_tag="PLZ54_07490"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ66804.1"
                     /translation="MLNIQKKMSNAFFALLSLPSTAMGFALSVQISALSWILSTKYGL
                     AIHEIGIVWAAGPIAGIIAQPIVGAISDKLWFMGGRRRPWIVLGGTLAALMLLALPNL
                     EVIQKIIVGGSDPQAGLLTIAIVVALTLDLSINVSFNPTRSLVADCTPLSERSKGFTW
                     MQTISGSFGVLAYLIGGTLGNIFLIYFGVGVAFLASVIPAFLIEEPRELKNFDAETQS
                     PKSKTTFRELWEAMLPLSGFLLYGIYVIFSKLGGFELKPTIPLFGYYLNVIEFLSILL
                     LLVSAIAVLLNLFKSGKKSNEFQKVLLAHSFTWIGVQTMFIYALFYLKDVLQLADNAG
                     SVNSIAFFVLSLVSAVLPVLVLNRLAEKYGMVKTHLSCIFIMALGYASILFFGHSVAV
                     YYFLIAIVGIGWGSVVSLPFAIMSEKVDQTKMGLYMGIFNLAVVLPQLVASFKMGEVI
                     NAAPNKSVIFAICAITLFISGILWLFVKNEK"
     gene            complement(4967..5218)
                     /locus_tag="PLZ54_07495"
     CDS             complement(4967..5218)
                     /locus_tag="PLZ54_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66805.1"
                     /translation="MGKEVSENTHRLLHQYSPEKTSFVNISDQDIKRVEDILNNRPRE
                     NWISSEYNLFFNGLMEPTCLIYSVVRVCVFNRCGLLNIK"
     gene            5527..>6801
                     /locus_tag="PLZ54_07500"
     CDS             5527..>6801
                     /locus_tag="PLZ54_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOJ66806.1"
                     /translation="MKNRYRSLFFAFFLALLPLSFTHAVKAYPFPVQITQPDGTVLTI
                     RIHGDEFHHIKTTVDGYLVKQNAKGYWTYASQDAAGRITPSEVIARNVSSRSAQDIQF
                     LKSLKISSSVSLQHVKASGRQFQKPQLSVRKNAFPLSNSPRSLVILVNFSDKSFVTSS
                     AQSAFTNLLNQSGYSANGATGSARDYFMASSYGKFSPEFDVVGPYTLSKTMQSYGAND
                     ADGYDVDPARMIVDACKAADNAGVNFSQYDTDNDGLIDNIFVYYAGYNEAEGASENTI
                     WPHRWVVYTSQESSTDYTYDGTVASVTFDGVRLYDYACTSELKGTSGSNMCGVGTFCH
                     EFGHVLGLPDYYHTAEDKTTLDYWNIMDAGNYLNDGRTPPVYSTYDRFYLGWLTPEEA
                     NTPSNLTLKPIYQGTTQPANTHQQSYLLSATTH"
BASE COUNT         2249 a         1313 c         1323 g         1916 t
ORIGIN      
        1 ttctttacct tttatttccc ctgaaaaagt tgcattaatt acttgaattt acgttactat
       61 aagttactta tgactgtaaa ttattaagga ttaaaaaatt tgcaaataaa attgtatctt
      121 tgtatccgaa aaataaaagt ttaaaagaaa aaattgtatg acagttgtta caaaaaccat
      181 cacaatgagt gatggacgag aaattgttat tgaaaccggt aaattggcca agcaggctga
      241 tggatcggta atggtacgca tgggtgatac catgttgctg gcaacggtat gttcggctca
      301 ggatgccaat gaaggaaccg attttatgcc gttatcggta gattacaagg aaaaattcgc
      361 tgcttttgga cgttttcccg gaggtttttt acgccgcgaa ggtcgtcctt ctgactatga
      421 aattcttatt tcccgcttgg ttgacagggc tttgcgtcca cttttcccag atgattatca
      481 tgccgaaact ttcgtgaaca ttatgctttg ttcggctgat ggggaaaata ttccggatgc
      541 gcttgcaggt ttggcagctt ctgctgcact tgccgtttcc gacattcctt tcaacgggcc
      601 cgtatcggaa gtccgcgtgg cacgcattga tggaaatttc gtagtgaatc caactttcaa
      661 tcagttggaa aatgccgata tggacattat tgtggctgct actatggaca atatcatgat
      721 ggtggagggc gaaatgaaag aagttagcga agacgatttg cttgatgcca tgaaagttgc
      781 tcatgaagcc ataaaactac actgcaaagc tcagttggaa ttaatggaga cagtaggcaa
      841 gactaaaaaa cgcgaatatt gccacgaaca aaacgacgaa gagctgaaaa aggacatttg
      901 ggaaaaaacc tacgataagg tttatgccga agcagcttcc ggaaatgccg acaaacattc
      961 gcgcagcgaa cattttgagg ccattatgaa cgaatatatt gcatcgttgg atactgaaac
     1021 agccgaagca aaaaaaccgc tgatcgaaag atattatcat gccatagaaa aagaagccat
     1081 gcgtcgggct atccttgatg aaggaaagcg tctggatgga cgtaaaacta ccgaaatccg
     1141 tccaatatgg tcggaagtag attatttgcc ggggacgcac ggttcttcca tttttacgcg
     1201 aggtgaaact caggcattgg ctaccgtaac actgggaacc aaaatggacg aaaagttgat
     1261 tgatgaacct ttaattcagg gaaaagaacg ttttctgctt cattacaatt ttccaccttt
     1321 tgccacaggt gaagcaaggg cttcgcgggg tattggccgt cgggaaattg gacatggcaa
     1381 cctggccatg agagccttaa aaaatgttat cccggacgat tatccttatg ttattcgcgt
     1441 tgtttccgat attttggaat ccaacggatc gtcttctatg gcaactgttt gtgctggtac
     1501 tttagcactg atggatgcag gagtgcagat caaaaaacct gttgccggta tcgctatggg
     1561 actgatttct gaaaatcagg gaaaaaatta tgccgtgctt tccgatattt tgggtgatga
     1621 agatcactta ggcgatatgg actttaaggt taccggcacc agagaaggta ttactgccac
     1681 acaaatggat attaaagttg atggactttc gtacgaaatt ttggaaaaag ctctgagaca
     1741 ggcaaaagaa ggacgattgc atatcctgaa caaaatgttg gaaaccatat ctgaaccgcg
     1801 tgccgacttc aaaccgcatg ttccgcgcat tgtggtaatg tttgtaccaa aagaaatgat
     1861 tggcgcaatt attggcccgg gcggaaaaat tatccaaaac attcagctgg aaacaggtgc
     1921 taccatcaca attgaagaaa ttggcgatca gggacgtgtg gaaattgctg ctaccaataa
     1981 ggactcaatt gaagctgcca tcaataaaat taaaggaata gttgctattc ctgaagttgg
     2041 tgaaacttat ataggaaaag taaaatccat tatgtcctat ggtgcttttg tagaatttct
     2101 acccggcaag gaaggtttgc tgcatatttc ggagattgaa tggaaacgta ctgaaagcat
     2161 ggaccaaacc ggtatcaagg aaggggacat gatagaagtg aaacttctcg atattgatga
     2221 aaagacgggt aaattcaaac tgtcacggaa agctcttctt ccacgtccac caaaagaaga
     2281 aaagcccagg aactaattaa gttaaacatt atcaaataag agagtgtccc aaaaggacac
     2341 tttctttttt ttagtatgtc gggcatggta ttaagctaat aggatgagag ttcctgcatg
     2401 tgccaagttg aaaagaaaaa aaattctatt aaagagctta cttttgaacc tatctggaga
     2461 ttattttaat caagattaaa tttccttttc gaaaacagcc ggtgtatcat tgcgacgaac
     2521 aaaacccatt tttatcaaat atttatggtg ctgcggatgt tccgatttgg caacaagttt
     2581 atgaatgccg gtttccttca actgttgcac attggttttg tagataaatt ctcccggttt
     2641 cagatctcta tattctgaag caacaaaatc aagctcaaca taaagacaac cattgtcgat
     2701 tgttccataa aaaactccag cagcaacggc gttacgcaac aaaagtaaag ctatggaaga
     2761 agttttatct tgtttcgttg aagcaagctc gaaatgagga aaaatctgta gaatttcctt
     2821 tttatggaat tcgataaaat aattaaaata agcatcgttt ggttcaacgg caagtgcttt
     2881 gaaagattct tgagccgaat tcatcctgat cagataataa atatttacca gtacaataaa
     2941 caaattaagg aatccaaccg gtaaagctcc aatagaaaat ccataaaagg aaaacacggc
     3001 agaacctatg agattcaacc atcgaagttt ttttatggaa ctcattgtca gagaaatagc
     3061 aactatgatg gatgccagat agccaatcca ttctaagagt ggaatgttta gaaacatgat
     3121 tattggtttt aaaatataat gattaaataa acaaaaaagt aaatctacat ctttagacgt
     3181 cgatttactt ttagcgcaaa agtaaatatt ttctgtttac ttttcaaata ttaattcaaa
     3241 aaaacataaa tccattgctc ttgaaaaaag aaaaattgct aaaaacttac ttttcatttt
     3301 tcacaaacaa ccacaatata cctgaaataa aaagcgttat ggcgcaaatg gcaaagatga
     3361 cggacttatt gggagcagcg ttgattactt cacccatttt gaaacttgcc accaattgag
     3421 gcaacactac ggcaagattg aatattccca tgtaaagtcc cattttggtt tgatccacct
     3481 tttcgctcat aatggcaaaa ggcaacgaaa cgactgatcc ccagccaatt cccactatgg
     3541 ctatcagaaa ataatatacg gcaacactgt gtccgaaaaa taaaatggat gcatatccca
     3601 atgccatgat aaaaatgcaa gacaaatgtg tttttaccat accatatttt tccgcaagcc
     3661 gattcaatac caaaaccggt aaaacggcac ttaccaaact taaaacaaaa aacgcaatgg
     3721 agttgaccga acctgcatta tctgccaatt gcaaaacatc tttcaaataa aaaagagcat
     3781 aaataaacat cgtttgcacg cctatccatg taaaagaatg tgccagcaag actttttgaa
     3841 actcgttgct tttctttccg gatttgaaaa gattcagcaa aacagcaatg gcacttacca
     3901 gcaaaagcag aatagataaa aattcgataa cattcaaata ataaccaaaa agagggatgg
     3961 ttggtttcaa ttcaaatccg cccaattttg aaaaaatcac gtaaataccg taaagtaaaa
     4021 atccggaaag cggaagcatt gcctcccaca attcacggaa agttgttttt gatttgggag
     4081 actgagtttc agcatcaaaa tttttcaatt cgcgaggttc ttcgataaga aaagccggaa
     4141 tgaccgaagc cagaaaggca acgccaacac caaaatatat aagaaaaata ttacccagcg
     4201 ttccgccaat aaggtatgcc aacacgccga aagaacctga aatagtttgc atccaagtaa
     4261 atcccttgct tctttcggac aaaggcgtac aatcggctac cagcgaacgg gtgggattaa
     4321 aactgacatt gatggacaaa tcgagcgtca gtgctaccac aatggcaatg gtcaaaagtc
     4381 cggcttgtgg atcacttcca cctacaataa tcttttgtat aacttcaaga tttggcaaag
     4441 ccagcaacat caaagctgcc aatgttccac ccaaaacaat ccaaggcctc cgcctgccgc
     4501 ccataaacca caatttatcg gaaatagctc caacaattgg ctgggcaata attcccgcaa
     4561 tcggtccggc tgcccaaaca atacctattt catgaatagc caatccgtat ttggtagata
     4621 aaatccaact taatgccgaa atctgcaccg acagggcaaa acccattgct gtagaaggca
     4681 aactgagaag agcaaaaaaa gcattgctca ttttcttttg aatgtttaac atggttgagt
     4741 ttttttataa atttattctt gaaaaatttg atggaataaa acgctgcaat gatattattt
     4801 ttttatgaat gaaaggaaaa tttgaatgaa aattcgataa aatttcagga caaacgtttg
     4861 cagagatgta gggataagca aaatttctta tcaaccacaa aaattagagt tgtaaattca
     4921 attagtaaat acaacttttt ggtaacacaa atttcgtaat aaaaattcat ttgatgttaa
     4981 gcaacccaca tctattaaat acacaaacac gaacaactga atatatcaga catgtgggtt
     5041 ccatcagacc attaaaaaat aaattatatt ctgatgaaat ccaattttct cttggtctgt
     5101 tgttcaaaat atcttctact cgctttatat cttgatcaga gatattcaca aaagaagttt
     5161 tttcaggaga atactgatga agaagtctgt gagtattttc agagacttcc tttcccacga
     5221 atgaaaaatt ttgaaaaaag aaatctacca tcaatgccga gctgatcatt ttatgtcagg
     5281 caaatttttt gtatattcgg tagtgatgta tttttgtcaa tctattttag aacatgaaga
     5341 ttttaaattt tctcgaaaaa accaacaatt tttcagaaaa ccattttaaa tttaaaatta
     5401 ttgttatctt tgcgacccta atattttcca aacaaattaa aatatggaaa caaaattttg
     5461 tagtatgttt ataataagca ggttagatag tttcagattt tattgttcaa caaaaaaatt
     5521 aaacccatga agaatcgtta tcgttctctt ttctttgctt tttttcttgc tttgttgcct
     5581 ctgagtttca ctcatgccgt taaagcctat ccatttcctg ttcagattac gcaaccggac
     5641 ggaactgtgc ttaccattcg cattcatggt gatgagtttc atcatattaa aacaactgtt
     5701 gatggctatt tagtaaaaca aaatgcaaaa ggttactgga cgtatgcttc acaggatgct
     5761 gccggcagaa tcactccaag tgaagttata gccagaaatg tttcaagtcg ttccgcacag
     5821 gatattcaat ttctgaaaag tttgaaaatt tcttcctctg tttccctgca acatgtaaaa
     5881 gcttccggtc gccaatttca gaagccacaa ctttctgttc gcaaaaatgc ctttcctctg
     5941 tcaaattcac cacgttcgct ggttattctg gtaaattttt ccgataaaag ttttgtaacc
     6001 tcttctgctc aatcggcttt taccaatctg ctcaatcaaa gcggttacag tgccaatggt
     6061 gctactggtt cggcacgcga ctatttcatg gcaagctctt acggaaaatt ttctccagaa
     6121 tttgatgtgg taggccctta cactttatca aaaacaatgc aaagttacgg agccaatgat
     6181 gcggatggat acgatgtgga tccggcacga atgattgttg atgcctgtaa ggcagccgac
     6241 aatgccggag taaatttttc gcaatatgac accgacaacg atggactgat agacaacata
     6301 ttcgtttatt acgccggcta caacgaggcg gaaggcgcat cagaaaatac gatttggcca
     6361 caccgatggg tagtttatac cagccaagaa tcttcaaccg attatactta tgatggtaca
     6421 gtggcttctg ttactttcga tggcgttcgg ctgtacgatt acgcctgtac ttcagaactg
     6481 aaaggaactt ccggttcaaa tatgtgtgga gtgggaactt tttgccatga attcggtcat
     6541 gttctggggt tacccgatta ttatcacact gccgaagaca aaacaacatt agactactgg
     6601 aacattatgg atgccggtaa ttacttgaac gatggaagaa cgcctcccgt ttattcgact
     6661 tacgaccgtt tttatttggg ctggctaact cccgaagaag ccaatacacc aagcaacctg
     6721 actctgaaac ctatttatca gggcacaacg caaccggcaa acacccatca gcaaagctat
     6781 ttactttcgg ctactacaca c
//
LOCUS       DAOSCH010000103         6704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_7325_length_6704_cov_5.745375, whole genome shotgun sequence.
ACCESSION   DAOSCH010000103 DAOSCH010000000
VERSION     DAOSCH010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7325_length_6704_cov_5.745375"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="PLZ54_07505"
     CDS             <1..675
                     /locus_tag="PLZ54_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOJ66807.1"
                     /translation="GEFLEVHQFYARNIIVGFARLNGASIGIVANQPNFLAGVLDCDA
                     SRKAARFVRFCDAFNIPILTLVDVPGFLPGSGQEYSGIIIHGAKLMFAYGEATVPKVT
                     VTIRKSYGGAHDVMSCKQLRGDFNYAWPTAEIAVMGASGAIEILEGKNIANIADPEEK
                     AKYIAEKVAEYNEKFSNPYQAASYGYIDDVIEPRNTRFRIIRAFETLQTKKVVNPLKK
                     HCNIPL"
     gene            679..1026
                     /locus_tag="PLZ54_07510"
     CDS             679..1026
                     /locus_tag="PLZ54_07510"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOJ66808.1"
                     /translation="MIMKLLMINSWEELLTAAAIGIMVVFSVLFVLVLLFELFQVIVL
                     KVAKKKVVKSGIDVDKIKDIPANEIAAISMALYLYLDSMHDEESNVLTIKRIERRYSP
                     WNSKIYGLNNLHR"
     gene            1026..1472
                     /locus_tag="PLZ54_07515"
     CDS             1026..1472
                     /locus_tag="PLZ54_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOJ66809.1"
                     /translation="MKKFDFNINGNDFHVYIKSIDGNIAEIEVNGTPYTVTLNQEVKV
                     SKTPILVRKEVPTTPSNMNVADKFNPVPSNQKPSSTVIKSPLPGNILRINVKEGDTFK
                     EGDVLLVMESMKMENNILAERNGKIVKVCVSVGKAVLQDEVLFEIE"
     gene            1474..2715
                     /locus_tag="PLZ54_07520"
     CDS             1474..2715
                     /locus_tag="PLZ54_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOJ66810.1"
                     /translation="MEGMKNFRYKIGFLALLLISLFPISIFAQSSGSENLVIESLQKF
                     WNNTGLANFEFKYLAMIVVGLVFIYLGIAHKWEPLLLVPIGFGILIGNVPFTDGFRIG
                     IYEQGSVMNILYSGVVQGWYPPLIFLGIGAMTDFSSLISNPKLMMLGAAAQLGIFVSF
                     IIAYAVGFSPAQSGAIGIIGGADGPTAIFISSRLAPELMGAIAVAAYSYMALVPVIQP
                     PIMKLLTTNKERKIRMKPPRQVTKLEKIIFPIIGLLLTTFISPSAMPLLGMLFLGNLL
                     KESGVTERLADTARNALLNSITILLGVTVGASTQADRFLTVDSLMIFGLGAFSFMIAT
                     AGGVLFAKIMNLFLKEGNKINPLIGSAGVSAVPDSARVSQDVGLSYDKSNHLLMHAMA
                     PNVAGVIGSAVAAGILLGILS"
     gene            2866..3402
                     /locus_tag="PLZ54_07525"
     CDS             2866..3402
                     /locus_tag="PLZ54_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOJ66811.1"
                     /translation="MHPFSIIEKYYVKDSPLYRVLVIHSQQVRDKALEVAKQHPELNI
                     DTEFVGEACLLHDIGIFLCHAPRIYCFGEHEYIEHGYLGSDLLKKEGLPQHALVAERH
                     TGTGFTKEEIRLKKLPLPDRDMLPLSLEEQLICYADKFYSKSKLNLQLSVEEIRQNLS
                     FYGKEKVEKFDSWHQLFG"
     gene            complement(3412..4887)
                     /locus_tag="PLZ54_07530"
     CDS             complement(3412..4887)
                     /locus_tag="PLZ54_07530"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOJ66812.1"
                     /translation="MKQKFLSILFLFVNYSFLISAQNNLSTNDTGEVQTIKVIDLDSL
                     LKNTKWIQNNETNSEIIELSEDSDTLKTKHLEKIKVLKDSFQLFVSPTALATIKSDSL
                     LYLSNPFFIELVYMGLPFDFDIKPKEDLLTLFHERKASTLEENAYRPIKVPTSQESLI
                     KLRQDARNEITRTAVGLYKRMFYELSNPYVKGNRPIQVKPIEKVEFVDKNKYVNGKER
                     KMHVPEFQVGPWRHKGSFLLQFSENYISKNWYQGGNSNLSVLGILSGQINYDDKKRTQ
                     WENSLEWRSGFYSVFGDTTALRTLNTNEDVLKINSKFGYKASGNWFYSGAIDFSTQFL
                     KNFKSVNSNELKASLFTPIRLNVNVGMDYKYKKIFSLMVSPLSLKYIYLSDTVKVNPN
                     LFGIQKGKNHLEQIGSSFKAVVSYAPSREIQLDSKFAFYTNYEKIEVDWEIVCNMIVN
                     RFVSTRISINPRYDNTMIMAEGERAKVQFKQLLSVGFSHKF"
     gene            complement(5565..6527)
                     /locus_tag="PLZ54_07535"
     CDS             complement(5565..6527)
                     /locus_tag="PLZ54_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66813.1"
                     /translation="MDEKNTKEINLLDLIKMFNAWVVKVGKSFINFVLYLLKAGYRKK
                     YLTLTLIVLCFAIGEYMARPSNRQYAADAMASLYGVDAFTMKEIAKQLEQSRANNQYT
                     SLGTKLLLPDSIAKNIVSFNSYFVIDYLNNSTPDMVDFANNHSLEDTLNVRMKDKIYF
                     RLVTRNISQVPQVEKAILNFFNSQPTVRLLYTARQNELKGKIDLSSAEMQRLDSLAKI
                     SYFKDNDQQLKWSKDRLIVGEQQKQLFYGDMLLLQKVKAEAESELLTYKQPVELPSGF
                     VVNPFPMNGRLKYGVYGILIGLGLGLALAVCLEQLQKVIQFFRK"
BASE COUNT         2010 a         1144 c         1222 g         2328 t
ORIGIN      
        1 ggagaatttt tggaagttca tcaattttat gcaagaaata tcatagtagg atttgcacgc
       61 ttaaatggag cttcaatagg tattgttgca aatcagccca attttcttgc cggtgtactt
      121 gattgtgatg cttcgcgtaa agccgcacgc tttgtacgtt tttgtgatgc ttttaatatt
      181 cctattctta cattggtaga tgtgccgggt tttttaccag gttccggtca agaatattcc
      241 ggaattatca ttcatggtgc caaattaatg tttgcttacg gtgaagctac tgtgcccaaa
      301 gttacagtaa ccatccgaaa atcctatggt ggagcacatg atgtaatgag ttgcaagcag
      361 ctgcgtggtg attttaatta tgcatggcct actgccgaaa ttgcagttat gggtgcttca
      421 ggggccattg aaattctcga aggaaaaaat atcgctaata ttgcagatcc cgaagaaaaa
      481 gccaaatata ttgctgaaaa agttgctgaa tacaacgaaa aattttcaaa tccttatcag
      541 gctgcttctt atggctatat tgacgatgtg attgaacctc gcaatacacg tttcagaatc
      601 attcgtgctt ttgaaacttt gcaaacaaaa aaggtcgtga atccattaaa aaaacattgt
      661 aatattcctt tataaaaaat gataatgaaa ttattgatga tcaactcatg ggaagaactc
      721 cttacagcag ctgcaatagg tataatggtt gttttttccg tcttattcgt tttggtactg
      781 ctttttgaac tttttcaggt aatagtttta aaagtggcaa aaaagaaagt agtgaaatca
      841 ggtatagatg tcgataaaat aaaggatatt cccgccaatg aaattgcagc catttctatg
      901 gcgttatatt tatatttaga cagtatgcat gatgaagaaa gcaatgtgct taccattaaa
      961 cgaatagaaa ggcgttattc gccatggaat tcaaaaattt atggactaaa taatttacac
     1021 cgataatgaa aaaatttgat tttaatatta atggtaacga ttttcatgtt tatataaaat
     1081 ctatagatgg aaatattgct gaaattgaag taaatggtac accttacacc gtaacattga
     1141 atcaagaggt gaaagtgagc aaaactccta tacttgtaag aaaagaggtg cccacaactc
     1201 ccagtaatat gaatgttgct gataagttta atcccgttcc ttctaaccaa aaacccagct
     1261 ctacggtaat aaaatctcca cttccgggaa atattttaag aataaacgta aaagaagggg
     1321 atacattcaa agaaggggat gttttgctgg tgatggaatc aatgaaaatg gaaaataata
     1381 ttcttgccga gcgcaatggt aaaattgtaa aagtttgcgt ttcggtagga aaagccgttt
     1441 tacaagatga agttttattt gaaatcgaat aatattgaag gaatgaaaaa ttttagatat
     1501 aaaattggtt ttcttgccct attgttgata agtttgttcc ccatcagtat ttttgcacaa
     1561 tcgtcaggca gtgaaaatct ggtgatagag agtttgcaga aattttggaa caatactgga
     1621 ttagccaatt ttgaatttaa gtatttggct atgattgtgg taggtttggt tttcatttat
     1681 ttgggtattg cacacaaatg ggaacctttg cttctcgtac caataggttt tggcattctg
     1741 attggaaacg ttccttttac tgacggtttc cgcatcggaa tttatgaaca aggttcggta
     1801 atgaatattt tgtacagcgg agttgtacaa ggatggtatc cacccttgat ctttttgggt
     1861 attggtgcta tgaccgattt ttcttctctg atttccaatc ctaagttaat gatgcttggt
     1921 gctgctgcgc aattaggaat ttttgtttct ttcattattg cttatgcagt aggattttca
     1981 cctgcccaat ctggggcaat tggaattatc ggaggtgctg atggcccgac agctattttt
     2041 atttcttctc gtttagcgcc cgaacttatg ggagctattg cggtggctgc ttattcttat
     2101 atggccctgg ttcctgttat tcagccacct ataatgaaac ttttaacaac aaacaaggag
     2161 cgaaaaatca gaatgaaacc tccccgacaa gttaccaaat tggaaaaaat tattttcccc
     2221 attatcggct tgttgttgac aacctttatt tcgcccagcg ctatgccttt gttgggaatg
     2281 ttgtttttgg gcaatctttt gaaagaatcc ggtgttactg aacgtttggc tgatacggct
     2341 cgcaatgctt tactcaattc tattaccata ttgttaggtg taactgttgg tgcttctact
     2401 caagccgatc gttttctcac tgttgattcg cttatgattt ttggcttggg agctttctca
     2461 tttatgatcg ctactgcagg aggtgttctt tttgccaaga ttatgaatct tttccttaaa
     2521 gaagggaaca aaatcaatcc tcttatcggt tctgccggtg tttctgctgt tcctgattct
     2581 gcgagggttt cacaggatgt cggtttgtct tacgataaat caaaccactt attaatgcat
     2641 gcaatggcac ccaatgttgc tggtgttatt ggttcggccg tggcagcagg tattttgctg
     2701 ggtatacttt cttaaatgtt tgattttgat ttttttacaa ataagctcat tgtgttttta
     2761 acagtgggct ttttatttga taagtttttt tgaaataaac tgtatattct cttgttattt
     2821 tttatgaatt gattgaaaaa acttttttaa caatttatta tggttattca tcctttttca
     2881 attatcgaaa aatattacgt taaagattct cctctttatc gtgtccttgt tattcatagc
     2941 caacaagtca gagacaaggc attagaagta gctaagcaac atccggaact taacatcgac
     3001 actgaatttg tgggggaagc ttgtctgctg cacgacatcg gaatttttct ctgtcatgca
     3061 ccacgaattt attgttttgg agaacatgaa tatatcgaac acggttattt gggttcagat
     3121 ttattgaaaa aagaagggct tccacaacat gctcttgttg ccgaaagaca caccggaacg
     3181 ggttttacta aagaagaaat cagactcaag aaacttcctc tccctgatcg cgatatgctt
     3241 cccctttcac tcgaagaaca gttaatatgc tatgccgaca aattttattc caaatcaaaa
     3301 ttgaatctcc agctttcggt agaagagatt aggcaaaatt taagctttta tgggaaggaa
     3361 aaagtcgaga aatttgattc ttggcatcaa ctttttggtt aatccggttt gttagaattt
     3421 atgggaaaat cctacgctga gtagttgttt gaattgtacc ttcgctcttt cgccttctgc
     3481 cattatcata gtgttgtcgt aacgcggatt gatcgaaata cgagtagaga caaaacgatt
     3541 tactatcata ttgcaaacta tttcccaatc gacttcaatt ttctcatagt ttgtataaaa
     3601 agcaaacttg gaatcgagct gtatttctct tgatggtgca taagaaacaa ccgctttaaa
     3661 cgaactacca atttgctcta aatgattttt tcctttttgt atcccgaaca aattggggtt
     3721 aaccttgaca gtgtcgctta gatatatata ttttaaggac aagggagaca ccatgagcga
     3781 aaaaatcttt ttatacttgt aatccatacc aacattgaca tttaaacgga ttggcgtaaa
     3841 cagtgaagct tttaattcgt tagaattgac tgacttgaaa ttttttaaaa attgagtaga
     3901 gaaatctatc gctccactat aaaaccaatt gccacttgcc ttatagccaa atttgctgtt
     3961 tattttcaaa acatcttcat tggtgtttaa ggttcgcagt gctgtagtat caccaaaaac
     4021 tgaataaaac ccgcttctcc actccagact gttttcccat tgcgttcgtt ttttgtcgtc
     4081 ataattaatt tgcccgctta aaatgcccaa taccgaaaga ttgctgtttc ctccctgata
     4141 ccagtttttt gaaatataat tttcggaaaa ttgtagcaga aatgaacctt tatgtcgcca
     4201 aggcccaact tgaaattcag gaacatgcat ttttctttct tttccattca cgtatttatt
     4261 tttgtcaaca aactcaactt tttctatagg ttttacttga ataggccgat taccttttac
     4321 ataaggattt gagagctcgt agaacatcct tttatataat ccgacggctg ttctggtaat
     4381 ttcatttctt gcatcctgac gcaatttaat taacgattct tgtgaagtag gtacttttat
     4441 gggtcgataa gcattttctt caagtgtgga agcttttcgc tcgtgaaata atgtgagtaa
     4501 atcttcttta ggttttatat caaaatcaaa aggaagaccc atatagacca gctcaataaa
     4561 aaagggattg gataaatata acaaagaatc cgatttaatc gtagctaaag cagttggaga
     4621 aacaaacagt tggaaagaat ctttcagaac ttttattttt tctaaatgtt ttgttttcaa
     4681 tgtatctgaa tcttctgaca attctatgat ttccgaattt gtttcgttgt tttgtatcca
     4741 ttttgtattc tttagcaaac tgtccaaatc aattactttg attgtttgaa cttcaccagt
     4801 atcgtttgtc gataagttgt tttgtgcact tatgaggaat gaatagttta caaataaaaa
     4861 taaaatgctc agaaattttt gtttcatgat gtttctttat tcgtcttttt gattggtaaa
     4921 gttcaacaaa attaaaaaaa aaatgattat cttgataatg acctattgta atttataaca
     4981 gcaaacagca actttgcgaa gataacatcg gcgtatcatc tgtaaaattc atatcaaaac
     5041 tcatttagat tgcagtgaaa atttttcttg gtaaattagc agtgagcatt tggagatgaa
     5101 taatccattt agtttttaat cggatttgag gttttaaagg aaagaatttt gcccttaacc
     5161 agagccttag acttcaccct gcgtccaaat atttttttct gcaggcaagt atatttataa
     5221 caataccggt aagtttgtgt tactcgttac cgttcttgaa tataaaaatt accggtgatg
     5281 agatttttta cattcgtaac gaggcttatt ttgtagccgt taacgttttg aaaaaattat
     5341 tgcacaaaag tccctttaag tactaaaatg ctctataaaa gagttttcgc ggggaattct
     5401 tttgttaaaa tcaaaagaag tttttgttcc tgctacgaag tggtatgaaa gcttgtataa
     5461 actatatttt tgaaaacaag taaaatataa ttgtataaat aaatttattg tgctctactt
     5521 caaaattagg tatattgttt ttttaatgta cagaaaaggt atttttattt tctaaaaaat
     5581 tgaatcactt tttgcaattg ttcgaggcag acggcaagcg ccaaacccaa gcccaaacct
     5641 atcaaaatac cataaactcc gtatttgagt cttccattca taggaaaagg attgactaca
     5701 aatcccgaag gcaattcaac aggctgttta taagtcagta attccgattc tgcttccgct
     5761 ttcacctttt gaagcaacaa catatccccg taaaaaagtt gtttttgctg ttcgcccaca
     5821 atcaaacggt ctttcgacca tttcagctgt tgatcgttgt ccttaaaata agaaattttt
     5881 gcaaggctgt ccaaacgttg catttcagcc gaagataaat caatttttcc cttcagttca
     5941 ttttgtcttg cagtataaag aagcctgaca gttggttgtg aattgaagaa atttaatata
     6001 gccttttcaa cctgtggtac ttgtgaaata ttgcgggtaa ccagccggaa atatatttta
     6061 tctttcatcc gaacgtttaa agtatcttcc agtgaatgat tattggcaaa atcaaccata
     6121 tcgggagtag aattattcag ataatcgatt acaaaatagg aattgaaaga aacaatgttt
     6181 tttgcaatac tgtccggcaa caacaatttg gttccaagtg aagtgtattg gttattggct
     6241 ctcgattgtt ccaattgttt tgcaatttcc ttcatggtaa aagcatctac gccatacagc
     6301 gaagccatag cgtcggcggc atactgtcgg ttacttggtc gagccatgta ttcgcctatg
     6361 gcaaaacaca atacaatgag agtcagggtc aaatattttt ttcgataacc tgccttcaaa
     6421 agatataaaa cgaagttgat aaaagattta ccaactttta ccacccaagc attgaacatt
     6481 tttatcaaat ccagcaaatt aatttctttt gtatttttct cgtccatatt gattaaaatt
     6541 atagtttgtc aaaattgatt gttatatcat tcttctttcc actgttcttg ttcataattt
     6601 tctttccaat catattgatc ttcttgttca ttatcttcat catcatcttc ttcttcccat
     6661 tcgggtttgg gtggctgtgg gcctttgtgc ctgtacacaa atgc
//
LOCUS       DAOSCH010000104         6554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_7521_length_6554_cov_7.591322, whole genome shotgun sequence.
ACCESSION   DAOSCH010000104 DAOSCH010000000
VERSION     DAOSCH010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6554
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7521_length_6554_cov_7.591322"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(415..552)
                     /locus_tag="PLZ54_07540"
     CDS             complement(415..552)
                     /locus_tag="PLZ54_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66814.1"
                     /translation="MEKACCLNVHKDSIFACILDEQGKKILEKRYGTLTADLMTPFVI
                     H"
     gene            788..1738
                     /gene="metF"
                     /locus_tag="PLZ54_07545"
     CDS             788..1738
                     /gene="metF"
                     /locus_tag="PLZ54_07545"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007764101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOJ66815.1"
                     /translation="MSIVDKIKSSSSTAFSFELLPPLKGDGIASVFKTIDLLREFDPK
                     YINITTHRSEVIFKQNDQGLFEQSDVRRRPGTVAIAAAIKSKYKIPVVPHVICSGFTK
                     EEIEYELIDLQFLGITDLLLLRGDKAKHEKDFVPTGHMHATDLQQQVNNFNKGIFLDG
                     SRMKALPAEPFSYGMAAYPERHEESPNTESDLYYTKMKVDGGAEYLVTQMFFDNQKYY
                     DFVEKCRNMGITVPIIPGIKPITLMNQLTVLPKIFHTEIPEEFASELRKCKTDAEATE
                     VGVEWCTKQASDLKAHGVPSIHFYSLMAAQSVKRVAKAVF"
     gene            1757..1990
                     /locus_tag="PLZ54_07550"
     CDS             1757..1990
                     /locus_tag="PLZ54_07550"
                     /inference="COORDINATES: protein motif:HMM:NF027444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4834 family protein"
                     /protein_id="HOJ66816.1"
                     /translation="MHLIFSILILIVFIGVLFLLFVLGFIRSFFNFGRKSKKRKQSKE
                     DNNIFTHSSKKKKIFDKNDGEYVDFEEIEEETK"
     gene            complement(2345..3073)
                     /locus_tag="PLZ54_07555"
     CDS             complement(2345..3073)
                     /locus_tag="PLZ54_07555"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007765149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOJ66817.1"
                     /translation="MTVFYAPEVAFNNVLPKEESQHAIKVLRLKKGDKIVIVDGKGNG
                     YLAEILFPDSNHCEFEILETLPDEGNVHYHLHIAIAPTKNVERFEWFVEKATEIGIHE
                     ITPIICKFSERKMIKPERLEKIVISACKQSMRAQFPVVHSLCSFQEVLTTYSAQQKFI
                     AHCYTDQIMKEFDCVRKRNFLKNLIEPSTKIFILIGPEGDFSIDEVKTAIEKGFDPVS
                     LGVGRLRTETAGIVACTTVALINQ"
     gene            complement(3157..4050)
                     /gene="folD"
                     /locus_tag="PLZ54_07560"
     CDS             complement(3157..4050)
                     /gene="folD"
                     /locus_tag="PLZ54_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOJ66818.1"
                     /translation="MYTLLDGKLISEQIKKEIAEEVQQMIKAGKKRPHLAVIIVGHDG
                     GSESYVAGKIKDCQICGFQSTLIRLEEDVTEQRLLQEVERLNEDTDVDGFIVQLPLPK
                     HISEQKMIEAINYKKDVDGFHPINVGRLCIGLPCYVSATPMGIMELLKRYRIETSGKN
                     CVVVGRSNIVGKPTAMLLMQKTYPGDATVTICHSHTKNLKEICQQADIIVAAIGQPEF
                     LKGDMVKEGAVVIDVGTTRVPSTETKSGFKLKGDVDFNEVAPKCSFITPVPGGVGLMT
                     RVSLMKNTLLAAKAEIYEKNG"
     gene            complement(4304..5728)
                     /gene="ahcY"
                     /locus_tag="PLZ54_07565"
     CDS             complement(4304..5728)
                     /gene="ahcY"
                     /locus_tag="PLZ54_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOJ66819.1"
                     /translation="MTDNKALFTSLPYKVADISLADFGRKEIELAEKEMPGLMAIREK
                     YAAQQPLKGARITGSLHMTIQTAVLIETLAKLGAEVRWCSCNIYSTQDHAAAAIAAAG
                     ISVFAWKGETLEDYWWCTLQALTFEGGKGPNLIVDDGGDATLMIHTGVAAEKDANVLN
                     RPAEGEDEKELLAILKKVLKENSNFWSNITPEIRGVSEETTTGVHRLYQMLAEGTLLF
                     PAFNVNDSVTKSKFDNLYGCRESLADGIKRATDIMLAGKVVVVCGYGDVGKGCAKSMR
                     AYGARVLVTEIDPICALQAAMEGFEVCTVEDALPEGNIYVTATGNRDIITIQHLEKMK
                     DGAIVCNIGHFDNEIQVDQLKKFPGIQKVNIKPQVDKYIFPDGHSVLLLADGRLVNLG
                     CATGHPSFVMSNSFTNQTLAQIELFTKKYEINVYRLPKHLDEEVARLHLGQLGVKLTQ
                     LTKEQAEYIGVNPEGPFKPEHYRY"
     gene            5862..6500
                     /locus_tag="PLZ54_07570"
     CDS             5862..6500
                     /locus_tag="PLZ54_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOJ66820.1"
                     /translation="MNIFVDIHTHGKSEFNPYSVRSLQIKEAALFFDTEERRFCSVGI
                     HPWNAEQENLEDLERLFRYSSDTRWIAIGECGLDKNISTPFATQKSIFEGQISYSESL
                     KKPLIIHCVGYFNELLEIRNKFRPEQLWIVHGFRGKPELAKQLLKTGISLSFGEKRNA
                     ASVAVTPIDRLYVETDESLLPVEQFYREIAEIKNCRPENLNAGKKLLHLKNI"
BASE COUNT         1934 a         1288 c         1221 g         2111 t
ORIGIN      
        1 attttgcggg ttatagccag taatgccttt tgcgatttca tccgttttga gagatgatag
       61 tactttttac ccaaaaacgt cttctttgcg cgggctgccg accacgatac ctgaattatt
      121 actgtgcaaa gatacacatg tcaatgtact tttggtcaaa cggcggaact gtcggctgag
      181 ttgacgcatc tgttgtaaaa tcccttcagg aacaaaactg ccctttatca aatctttctg
      241 aagacattcc gctatccact gtgcatcctt tacgtcactc ttgcgaccgg gcagctgttt
      301 taatgaaata gggattggct agttttaaag aaaaatccga tgtaagaacc tgccagatcg
      361 gtatccaata aatgctgcta ctctccatcg ctacttttcc gcaaccttgt tctatcagtg
      421 aatcacgaaa ggggtcatta aatcggcagt gagtgttccg taacgttttt ctaaaatttt
      481 tttgccttgt tcgtccaaaa tgcatgcaaa tatgctatct ttgtgaacgt taaggcaaca
      541 tgctttttcc ataaaattac cggattaaat tgttaaacaa ttagtttatt tcattggtta
      601 ttaaaaacca acttacaaag gtaacatttg tgctcagaag cacggtacgg taaaccttta
      661 aagtcttgtg accttgattt ttattttcac acgtttggaa atacctcgat tttcatgtaa
      721 ttttgcaaat taaatttctt tattcctcgt ttttttattg taagtttctt acgtttcctt
      781 ttaagatatg agtattgtcg acaaaataaa aagttcttct tccacagcat tttctttcga
      841 gcttttgcct ccattgaaag gggatggcat agcttccgta tttaaaacca tcgatttact
      901 aagagaattc gatcctaaat atatcaatat tacaacgcat cggagcgaag ttattttcaa
      961 acaaaacgat cagggacttt tcgaacaatc agatgttcgt cggcgtcctg ggacggtcgc
     1021 tattgccgct gccataaaaa gtaaatataa aattccggtt gttccgcatg tgatatgcag
     1081 tggatttaca aaggaagaaa ttgaatatga acttattgat ttgcaatttt tgggaatcac
     1141 tgaccttttg ctgctcagag gagataaagc caagcatgag aaggattttg ttccaacggg
     1201 acatatgcat gccaccgatc ttcagcaaca agtcaacaac ttcaacaagg gtatttttct
     1261 ggatggcagc agaatgaaag cacttcccgc agagcctttt tcctatggaa tggcagccta
     1321 tcctgaaaga catgaagaat cgcccaacac cgaatcggac ttgtattata ctaaaatgaa
     1381 agtggatggc ggagccgaat atctggttac ccaaatgttt ttcgacaacc aaaagtatta
     1441 cgattttgta gaaaagtgca gaaacatggg gattaccgtt cctattattc caggtataaa
     1501 accaataact ttaatgaatc agctcacagt gctgcctaaa atttttcaca ccgaaattcc
     1561 tgaagagttt gcgtctgaac ttcgcaagtg taaaaccgat gcagaggcaa ccgaagtggg
     1621 ggtggaatgg tgtaccaagc aggcctccga tttaaaggct catggtgtac caagtattca
     1681 tttttacagc ttaatggctg cacagagtgt taagcgtgtt gctaaagccg ttttttaaaa
     1741 tattcaatct ctatttatgc atcttatttt ttccatactg attcttattg tctttattgg
     1801 agtacttttc cttctttttg tgttgggatt cattcggtcg ttttttaatt ttgggagaaa
     1861 aagtaaaaaa agaaaacaat ctaaggaaga taacaacatt tttacacatt catcaaagaa
     1921 gaaaaaaatt ttcgacaaaa acgatggaga atacgttgat tttgaagaaa ttgaagaaga
     1981 aacaaagtaa gcggataaat ccagaaaaaa tttgttccga taaatttctc tcacatttgg
     2041 gcatgggcta caaatagttt cgtccttaag agatttactc attagccaat tcaaagggaa
     2101 agtcaaagtc ggaagagttg ttcgatagtc aggaaaacca agctcgtttt tttattgaaa
     2161 gcaattttgt taaacagccc tgctgatttt tttatatcca gctactggag aagttctgca
     2221 taaaagtttt atatggtaga taaaatttta tctgttttat acaagattaa aacctttttt
     2281 cctattgtgg agttgggttt aattgcttga atttacgaga gaatataatt gtcccaatat
     2341 aaatttattg attgataagc gctacggtcg tgcaggccac aattccagct gtctctgttc
     2401 ttaaacgccc cactccgaga gaaacaggat cgaatccttt ttcgatggct gtttttactt
     2461 cgtcgatact gaagtcgcct tcaggaccaa tcaaaataaa tattttcgtg gatggttcta
     2521 tcagattttt taaaaaattt ctctttctca cacagtcaaa ttctttcatt atctggtcgg
     2581 tataacagtg tgctatgaat ttttgctgtg ccgagtaggt tgtcagtact tcctgaaaac
     2641 tgcaaaggga atgaacgaca ggaaattgtg ctctcattga ttgcttgcag gcagaaataa
     2701 caattttctc aagtcgttcg ggcttaatca tctttcgctc ggaaaactta caaataatag
     2761 gggtaatttc atgtatccct atttcggttg ctttctcaac aaaccactca aaacgctcga
     2821 catttttagt gggagctatg gcaatgtgta aatgataatg aacgtttcct tcatcaggca
     2881 aagtctcaag aatttcaaat tcgcaatggt tactgtctgg gaaaagaatt tcagcaaggt
     2941 agccattccc ctttccatca actatcacta ttttatctcc tttttttaag cgaagaactt
     3001 ttatggcatg ttgtgattct tctttgggta aaacattgtt aaaagcaact tcgggagcat
     3061 aaaaaacggt cataattatc aaaaagtaac gataaaattt attgtgtcaa aacaggcaat
     3121 ataagttgaa ccaaatttat ccgaaaaaaa ttatatttat ccatttttct cataaatttc
     3181 ggctttggca gccaacaagg tgtttttcat aagcgaaacg cgagtcatta atcctacacc
     3241 tcccggcaca ggagtaataa atgaacattt tggagctact tcattgaaat ctacatctcc
     3301 ttttagtttg aaacccgatt ttgtttcggt tgagggaact cttgttgttc caacatcaat
     3361 aaccactgct ccttctttta ccatatcccc ctttaaaaac tctggttgcc ctatggctgc
     3421 cacgataata tcggcttgtt ggcaaatttc tttcaagttt ttggtatgac tgtggcaaat
     3481 cgtaacggtt gcatcacccg gataagtttt ttgcatcaaa agcatggcag taggcttacc
     3541 tactatgtta ctgcgcccaa caacaacaca gttttttcca ctggtttcta tccgataacg
     3601 cttcaataat tccataattc ccattggcgt ggccgaaaca taacacggta atcctataca
     3661 taatcggcca acattgatcg gatgaaaacc atctacatct tttttataat taattgcttc
     3721 aatcattttt tgttccgaaa tatgtttcgg taaaggtaat tgaacaatga atccatcaac
     3781 atcagtatct tcatttaaac gttccacttc ttgcagcaaa cgttgttctg ttacatcttc
     3841 ttctaatcga atcagagtgg actgaaatcc gcagatttgg caatctttga tctttccggc
     3901 tacatacgat tcacttccac catcatgacc aacaataatc actgccagat gaggacgctt
     3961 tttaccggct tttatcattt gttgaacttc ttcagcaatt tcctttttta tctgttccga
     4021 aattagtttt ccatccaaaa gggtatacat gttataactt gattaaatgt ttttaggcat
     4081 atcaatttca tcagaatata atttattttt taaaggtctg atggaaccca cctgtctgaa
     4141 tattatattc attcgtgttt gtgtatgcaa tacatgtggg tttcttattt ttacggtttt
     4201 tagtttgttt gtagaaaaag gtaattgagt taaaaagaat gaattgaaaa taaaggaaaa
     4261 caacaatatc ttcgtggaaa atacgattaa ttttttaaac atttcagtaa cggtaatgtt
     4321 ccggtttaaa aggtccttcg ggattcacac caatatattc tgcctgctct ttggtaagtt
     4381 gggtaagctt tactcccaat tggcctaaat gcagtcgggc aacttcttcg tccaaatgtt
     4441 taggtaaacg ataaacattt atttcgtatt tttttgtaaa cagctcaatt tgagccagtg
     4501 tttggttggt aaaggaattg ctcatgacga aagaaggatg acctgttgca cagccaagat
     4561 ttacaagccg tccgtcggca agaagcaaaa cactgtgtcc atcggggaaa atatacttat
     4621 ccacctgcgg tttgatgttt actttctgaa tgccaggaaa ttttttcaac tgatccacct
     4681 gaatttcgtt gtcgaaatgt ccgatattgc acacaatagc gccatccttc attttttcca
     4741 agtgttgaat ggtaataata tcgcgattgc cggtggcagt aacataaata ttcccttcag
     4801 gcaaagcatc ttctaccgta cacacttcaa atccttccat tgcagcttgc aaagcacaaa
     4861 tcgggtcaat ttcggttacc agcactcttg ctccataagc ccgcatggat ttggcacaac
     4921 ctttccccac atcaccataa ccacaaacta ccacaacttt gcctgccagc atgatatctg
     4981 ttgccctttt tatcccgtcg gccaaagatt cacggcagcc ataaagatta tcaaatttcg
     5041 atttggtaac ggagtcgttg acattgaaag cggggaacag caatgttcct tccgcaagca
     5101 tctgataaag ccgatgaaca cccgtagtgg tttcttccga aacgccacga atttccggag
     5161 ttatattcga ccagaagttg ctattttcct ttaaaacttt tttcaatata gccaacaatt
     5221 ctttttcatc ttctccttcg gcaggccggt tcaatacgtt tgcatctttc tctgctgcca
     5281 cacctgtatg aatcataagc gttgcatcgc cgccatcatc aacaattaaa ttcgggcctt
     5341 taccgccttc aaaagttaaa gcctgcaagg tgcaccacca gtaatcttcc agtgtttcgc
     5401 ctttccaagc aaaaacagat attcctgctg cggcgatggc tgcagcggca tgatcctgcg
     5461 tggaataaat attgcaactg caccagcgta cttcagcgcc aagttttgct aacgtttcaa
     5521 ttaaaacagc cgtctggata gtcatgtgaa gtgagccggt aatgcgggca cctttcagtg
     5581 gctgctgggc tgcatatttt tctcgaattg ccataagtcc cggcatttct ttttctgcca
     5641 gttctatttc ctttctgcca aaatcggcca acgaaatatc ggccaccttg tacggcaaag
     5701 aagtgaacaa tgctttattg tctgtcattt tgttgattct tttgttcaaa tatcctttaa
     5761 atctaatgaa aagtttgcaa agatatgaat tttacttgaa aattctttac ttttgcatca
     5821 atgttttcaa aggtagccgc agttcataga ggccgataaa gatgaacatt tttgttgata
     5881 tccatacaca tgggaaatct gaatttaatc cgtattctgt gagaagttta cagattaagg
     5941 aggcagctct tttttttgat actgaagagc ggcggttttg ttcggtgggt attcatcctt
     6001 ggaatgcaga acaggaaaat cttgaagatt tggaaaggtt gtttcgttat tcttccgata
     6061 cacggtggat agcgataggt gaatgtggac tcgacaaaaa tatttccact ccttttgcca
     6121 cgcagaaaag catttttgaa ggtcaaattt cttattccga aagtttgaaa aaacctttga
     6181 ttatccattg tgtcggttat ttcaacgagt tgttggaaat taggaacaag tttcgacccg
     6241 aacaactttg gattgtgcat ggcttccgcg gaaagcccga attggccaag caattattaa
     6301 aaaccggaat ttctctttca tttggcgaaa aacggaatgc ggcaagcgtg gcagtaacgc
     6361 caattgacag actttatgtg gaaacagacg aaagtttatt gccggttgaa cagttttatc
     6421 gagaaattgc agaaattaaa aattgtcggc ccgaaaattt gaatgcggga aagaaattgc
     6481 ttcatttgaa gaatatttga atcgattcag ctaaagttgc aattttcaaa gagataaacc
     6541 gaaaaaaatt aatt
//
LOCUS       DAOSCH010000105         6324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_7853_length_6324_cov_4.873824, whole genome shotgun sequence.
ACCESSION   DAOSCH010000105 DAOSCH010000000
VERSION     DAOSCH010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6324
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7853_length_6324_cov_4.873824"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..2859
                     /locus_tag="PLZ54_07575"
     CDS             193..2859
                     /locus_tag="PLZ54_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ66821.1"
                     /translation="MRKVFSFLFMMMLAQFAFSQASVKGVVVDKSTNEPLVGVSVLQP
                     ALGIGTTTSLDGSFSLRLPEGKHELRFSYVGYSTKIVQVSSNNVNLGFVQMESEAIGL
                     NDVTVTSSLAIRRKTPVALSVIEPLVIEEKLGTQEFPEILKSTPGVYATKQGGGFGDS
                     RINLRGFESANIAVMINGVPMNDMEWGGVYWSNWAGLSDVTRSMQVQRGLGASKVAAP
                     SVGGSINIVTKSTDAKKGGSFSYSIGNDGYNKISFNVSTGLSETGWAMTFLGAKTWGN
                     GYIQGTEFEAYSYFINISKIINEDQQISFTGFGAPQWHNQRYNGDKLLIPEWQKLKDG
                     YKFNPTYGFDENGQRKTGNYNHYHKPQLSLNHYWNMNDKSSLSSALYLSIGNGGGYGW
                     RGNTGTLMYGTNTATGLLNTTYRTLDGYMDYGKLQAENAANLNGSQAVIADSRNNHTW
                     LGLLSTFTTKFGQNIDFYGGLDLRYYAGMHDAVLVDLMGGKFFIDPARVNVKNSVNAG
                     NPAYVNEKLQVGDIVYRDNTGYVAQEGVFSQAEYSKGKLNVFLAGSLSNSTYWKVDRF
                     YYDNQKSDSKSFIGFTAKTGANYNLTDKHNIFANIGYISRAPFMSGGYFVTIHTSNAV
                     NKNAVNEKVFSTELGYGYRSKFLTANVNLYRTAWMDKTMVRSFGSNSDDGVVNLKGVN
                     ALHQGLEIDLISKPVKNLELTGMLSLGDWEWTSNATGYVFNKDGQPVNASGSTVDKNG
                     NTIQMFSADHAFVNLNLKGVKVGNSAQTTFALGAKYNFLKDFSAGLDYTHYARNYANF
                     SITANVGTTNYTTPWQIPAAGVFDFNANYKFKLQGFDASLNGVINNLFNQVYITDAQD
                     LTPTTSTSDEWKNTAVLYGFGRTYSISLRVRF"
     gene            2963..4879
                     /locus_tag="PLZ54_07580"
     CDS             2963..4879
                     /locus_tag="PLZ54_07580"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOJ66822.1"
                     /translation="MKKLLIFILSSIMIALTFSSCDYNEENFPGLDEMAAYKDVVNYE
                     YTISESDFTTIISALRANKNAQDSATATLLSSSKIFSVSAPASTLVPYVLKSKFYGAT
                     SGSAAKVTYQYKTGSYQYYLLTQADYKTIWQQDFIYALTPSKSPQSVLPGFLSQKFPA
                     AKEGDIKILEYSFSSADPVTSEVPLQYFKEDFESYEAGSGKTIPASAGNFFILNKDFL
                     GTRTWECRIFSNNKYAQVTSNASNTENQVWLITKQIDLTDANVPSLTFDVNVGYYNAD
                     CLSILISENFDGTEAGVASASWTDVTSSFTLPQTPTSGYGTLASAGTLELSAFKGKKI
                     NVAFKYTGDGRTDANPKKTTTYQVDNVVVSDTKTVSTVSDPKTQFSYYIYQNGQWNFV
                     NASFYQLAEEDYTSMGVATLNATTAPNYLPILLKIKFPFAQEGATKVVVYKTSATKNA
                     ADEYIYKSGVWSPISLIETRTEQFVFAGWENGGWIFDPTLRVTMKKGTNPTDDYMLVV
                     NYVRDTYGAQTPSLLGYYGTSLQSEYYYGFAAYYGNISWRESDRAKDPTYAALTTAEE
                     KQNYLVERTKEGLAVYLSLKFPDAQPKVSGVDLYCYVTTAIYNGVTTTNYVYKYQRVD
                     GEPFGWKYIESNPL"
     gene            5137..6306
                     /locus_tag="PLZ54_07585"
     CDS             5137..6306
                     /locus_tag="PLZ54_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66823.1"
                     /translation="MKKILIAILMLIVLMPLIMWLLWIIKKPRELNILILDKTVLTSA
                     VQEHLSLSWVINNEKFIHNSKSYNKQKDYYGFFPDDKGNYHIRDFENFSSENLDNLAN
                     SYDGAYYTDMYGIYANEWIIRYYPEKIHDKRFIADRSKKIYGGMTQKELDFLRLMKQK
                     NKLIINEFNVIASPTSKEIRNKYENEFNIHWTGWVGRYFDNLDTLVNEDIPRWLIDNY
                     TRNHSGQWPFKKSGIAFVSENDQILILEKDTHLNTDIPYIYTNENNAKHYHLPEKIKY
                     PFWFDIMETNPSFRIISSYHLDTNAAGDSLLALGRIPKIFPAAVNYKNGYNYYYFCGD
                     FADNPISYASSRFRYIQVFSPLFYSSSKNERASFFWKYYRPLLRQILNEEYQRTE"
BASE COUNT         1947 a         1180 c         1258 g         1939 t
ORIGIN      
        1 atcagggcaa aggggaaatg ttcttttttc tcctttgtct tgaaaaataa ttttttcttt
       61 accttttatt tcccctgaaa aagttgcatt aattacttga atttacggtt ttaatgatat
      121 gtgatattaa aaaaatagaa tatatttgcc atgcttatct taaaaaatta aaaactaaaa
      181 aagagaattc ttatgaggaa agtattttca tttctattta tgatgatgct tgcgcagttt
      241 gcattttcgc aagcttcagt taaaggggtg gtagtggata aatctaccaa tgaacctttg
      301 gttggagtaa gtgttcttca acctgctttg ggaattggga caacaacttc gcttgatgga
      361 agtttttctc tgagattgcc ggaaggaaaa cacgaactta ggttttctta tgtaggctat
      421 tctacaaaga ttgttcaagt ttcttcgaac aatgtcaatt tgggatttgt acaaatggag
      481 tcggaggcca ttggtttgaa tgacgtaact gttacttctt cgttggcaat tcgccgtaaa
      541 acgcctgttg ctctgtcagt aatagagcct ttggtaattg aagaaaagct gggtacgcag
      601 gaatttcctg aaatattaaa atccactccg ggtgtatatg caaccaaaca aggtggtggt
      661 tttggcgatt cacgtatcaa tttacgtggt tttgaatcgg ccaatatcgc cgtcatgatc
      721 aatggagttc ccatgaatga catggagtgg ggaggcgttt actggtcgaa ttgggccgga
      781 ctaagcgatg ttacccgttc tatgcaggtt caaagaggtt tgggcgcttc caaagttgca
      841 gctccttcgg taggaggttc catcaacata gttaccaaat cgaccgatgc aaaaaaaggt
      901 ggctcttttt cctattccat tgggaatgat ggctacaata aaatttcgtt caatgtctcc
      961 accggacttt ccgaaacggg ctgggcaatg acttttctgg gagcaaaaac atggggaaat
     1021 ggttatattc aaggaaccga gtttgaagcc tattcctatt ttataaatat ttcaaaaatt
     1081 ataaatgaag atcaacagat ttcttttacc ggttttggag cgcctcaatg gcacaatcag
     1141 cgatacaacg gcgacaagct gctgatacct gaatggcaaa aactcaaaga cggttataaa
     1201 tttaatccta cttatggttt tgatgaaaac ggacagagaa aaacggggaa ttacaaccat
     1261 tatcataaac ctcaactgtc attgaatcat tattggaaca tgaatgacaa atcgagtctt
     1321 tcttcggctt tatatctttc cataggtaat ggaggcggtt atggttggag aggaaacaca
     1381 ggcactttga tgtatggaac caatacggca accggtttgt tgaataccac ttaccgtact
     1441 ttggatggtt atatggatta tggaaaatta caggcagaaa atgcagccaa tctgaatggt
     1501 tcacaagccg taatagccga ttcgagaaat aaccatacct ggctgggttt gctttcaact
     1561 tttactacca aattcggaca aaatatagat ttttacggag gactcgactt gcgttactac
     1621 gccggaatgc acgatgctgt attagtggat ttgatgggtg gaaaattctt tatcgatcct
     1681 gcaagagtga atgttaaaaa ctcggtgaat gccggtaatc ctgcatacgt caacgaaaaa
     1741 ctgcaagtag gagatatcgt ttatcgcgac aatacgggtt atgtggctca ggaaggcgtt
     1801 ttttcgcagg ccgaatattc caaaggaaaa ctgaatgtgt tcttggccgg ttctttgtcg
     1861 aacagcactt actggaaagt ggaccgtttt tattacgaca accaaaaatc ggattcaaaa
     1921 agctttatcg gttttaccgc caaaaccggt gccaactata accttaccga caaacacaat
     1981 atttttgcca atatcggtta tatttcacgc gctccgttca tgtcgggcgg ttattttgta
     2041 actattcata ccagcaatgc cgtaaataaa aatgcagtca acgaaaaagt tttttcaact
     2101 gaattgggat acggttatcg ttcaaaattt ttaacggcaa atgtaaatct ttatcgtacg
     2161 gcttggatgg ataaaactat ggttcgttct ttcggctcca attcggatga tggcgttgtc
     2221 aatctgaaag gtgtaaatgc tcttcatcag ggtttggaaa ttgaccttat aagtaagcct
     2281 gttaagaatc ttgaacttac cggaatgctg tcgcttggag actgggaatg gaccagcaac
     2341 gctacgggat atgtgtttaa caaagatgga cagccggtca atgcaagtgg ttcgacagta
     2401 gataaaaatg gaaataccat tcaaatgttt tcggccgatc acgcatttgt caatttgaat
     2461 ttgaaaggtg ttaaagttgg taactcggct caaaccactt ttgctttggg tgcaaagtat
     2521 aatttcctga aagatttttc agcaggattg gattatacgc attatgcccg caactatgcc
     2581 aattttagca ttacggccaa tgttggaaca accaattata ccacgccgtg gcaaattcct
     2641 gctgcaggag tttttgactt taatgccaat tacaagttta aacttcaggg attcgatgct
     2701 tccttgaatg gggtgatcaa taatttgttc aatcaggtat atattactga tgctcaagac
     2761 ttgacgccaa caacttctac ttctgacgaa tggaaaaata ctgcggtgtt gtatggtttt
     2821 ggaagaacct attctatcag tttgcgagtt cgattttaag taaaaataat ttttagtcat
     2881 taagataaaa caactcaaga aaaaaaactg gattttcagg tatttttcag caagttttac
     2941 aaataaatta aaattacgac gtatgaaaaa gcttttaatt tttatcctgt catccataat
     3001 gattgcattg accttttcgt cgtgcgatta taatgaggaa aattttcccg ggttggatga
     3061 aatggcagcc tataaagatg tagtcaatta cgaatatacg atttccgaat ccgactttac
     3121 caccatcatt tcggcattgc gagccaataa aaatgcccaa gattctgcca cagccacttt
     3181 attgagcagt agcaaaattt tctctgtaag cgctcctgct tccactttag tgccttatgt
     3241 gttgaaaagc aaattttatg gcgctacaag cggttctgct gcaaaagtta cttatcagta
     3301 taaaacaggt tcgtaccagt attatctgct tacccaggcc gattataaaa ccatttggca
     3361 gcaggatttt atttacgcac ttacaccttc caaatctcca caaagtgtgt tgccgggatt
     3421 tttatcgcaa aagttccctg cagcaaagga aggcgatatt aaaattttgg aatattcctt
     3481 ttcttctgcc gaccctgtaa catctgaagt gccgcttcag tactttaaag aagattttga
     3541 atcttacgaa gctggttctg gaaaaacgat tcctgcttct gccggcaatt tttttattct
     3601 gaacaaggac tttctgggaa caagaacgtg ggaatgtcgt atattttcaa acaataaata
     3661 tgcacaggta acctcgaatg cttcaaacac cgaaaatcag gtttggctga taaccaagca
     3721 aattgacttg acggatgcca atgttccgtc gttaactttt gacgttaatg ttggttacta
     3781 taatgcagat tgtttgagta ttctgatttc ggaaaatttt gatggcacgg aagccggtgt
     3841 ggcttcagct tcatggacag atgttacatc aagttttact cttccacaaa ctccaacttc
     3901 cggttatggt acgttagctt ctgccggtac gcttgagctt tctgcgttta aagggaaaaa
     3961 gattaatgtg gcattcaaat ataccggaga tgggcgaaca gatgccaatc cgaaaaaaac
     4021 caccacttat caggtcgata atgtcgttgt ttcggatacc aaaacggtaa gcactgtttc
     4081 agacccaaaa actcaatttt catattatat ttatcaaaac ggacagtgga attttgtaaa
     4141 tgcttctttc taccaattgg ctgaagaaga ttatacttca atgggtgttg ctactttgaa
     4201 cgctactaca gctccaaatt atttgccgat attgctcaaa atcaaattcc cgtttgccca
     4261 ggaaggagct acgaaagtgg tggtttacaa aacaagcgcc acaaaaaatg cagccgatga
     4321 atatatttac aagtcgggag tttggtcgcc tatttcgctg attgaaacca gaacggaaca
     4381 atttgttttt gcaggttggg aaaatggtgg ttggattttt gacccaactt tacgcgtaac
     4441 catgaaaaaa ggcaccaatc cgacggatga ctatatgttg gttgtaaact atgtaagaga
     4501 tacatatgga gcacaaactc catccttgct gggatactat ggaaccagtc tgcaatcgga
     4561 atattattat ggctttgcgg cttattacgg caatatttcg tggcgggaat ccgaccgagc
     4621 taaagatccg acttatgcag cactaaccac tgcagaagag aaacagaatt atcttgttga
     4681 gcgcaccaaa gagggacttg ccgtttatct gagtttgaaa tttcccgatg ctcagccaaa
     4741 agtcagcggc gtagatttgt attgttatgt aaccactgct atttacaacg gcgttaccac
     4801 aacaaattat gtttacaagt atcagcgtgt tgatggagaa ccctttggct ggaagtatat
     4861 agaaagcaat ccgttgtaat ttttcttcta ctttttagtg ttttttcaaa gagccggcgt
     4921 ttatctgccg gctttttggt taagcatatt tgcagcttgt ccttgttcaa ccattctaaa
     4981 aaaataactt tgtaccaaaa acagttgcca tataaatcta tcccatgctg ataccaatgt
     5041 tcgaaaaaat taaaaaatat tttcaataaa ttggctaaaa agtaaaactt ttattttttc
     5101 tatttgttaa actattgaaa aaaattaaat aaatgaatga aaaaaatact gattgcgatt
     5161 ctgatgctca ttgtactaat gcctctcata atgtggcttt tgtggataat taaaaaaccg
     5221 agagagctga atattctgat tttggataaa accgtattga cttctgccgt acaggaacat
     5281 ttgtcgcttt cgtgggtaat caacaacgaa aaatttattc acaacagcaa atcttacaac
     5341 aaacaaaaag actattacgg ctttttccct gacgataagg gaaattatca tattcgagat
     5401 tttgaaaatt tttcctctga gaatctggac aatttggcaa atagctacga cggtgcttac
     5461 tatacagata tgtacggtat ttatgcgaat gaatggatta tccggtatta tcccgaaaaa
     5521 attcacgata aaagatttat tgccgatcgt tccaaaaaaa tttacggtgg catgacacaa
     5581 aaagaacttg attttcttcg attgatgaaa caaaaaaata aacttatcat taacgaattc
     5641 aatgtgatag cctctcctac ctcgaaagaa atacgcaaca aatacgaaaa cgaatttaac
     5701 attcattgga ccggttgggt gggacgctat ttcgacaatc tcgacaccct cgtgaatgag
     5761 gatattcctc gctggctgat cgacaactac acccgcaacc acagcggaca atggcctttt
     5821 aaaaaatcgg gaatcgcttt tgtcagtgaa aatgaccaga tactgattct ggaaaaagat
     5881 acccatttga ataccgacat tccttacatt tacaccaatg aaaataatgc aaaacattat
     5941 catcttcccg agaagatcaa atatcctttc tggttcgata ttatggagac aaatccttcc
     6001 tttcgaatta tttcttctta tcatctcgat accaatgctg ccggcgattc gctgttggcg
     6061 cttggcagaa tacccaaaat atttccggcg gcagtgaatt ataaaaacgg ctacaattat
     6121 tattatttct gtggcgattt tgccgacaat cccatttctt atgcaagttc gcgtttccgt
     6181 tatatccaag tgttttcacc acttttttat tcttcttcca aaaatgaaag agcaagtttt
     6241 ttttggaaat attatcgtcc cctgcttcga caaattttaa acgaagaata tcagcgaaca
     6301 gaataaacac aatttttaaa attc
//
LOCUS       DAOSCH010000106         6312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_7867_length_6312_cov_6.236215, whole genome shotgun sequence.
ACCESSION   DAOSCH010000106 DAOSCH010000000
VERSION     DAOSCH010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7867_length_6312_cov_6.236215"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1382
                     /gene="thrC"
                     /locus_tag="PLZ54_07590"
     CDS             48..1382
                     /gene="thrC"
                     /locus_tag="PLZ54_07590"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HOJ66824.1"
                     /translation="MKYYSTNKGISGVSLKDAVIKGLADDKGLFMPEQIKQLPPSFFD
                     TIINMSFQEIALAVAEAFFGEDVDNEALKNIVYETLSFDVPLVHVKDNIFSLELFHGP
                     TLAFKDVGARFMSRLLRYFIQKEGEKNVNVLVATSGDTGSAVANGFLGVEGIHVFVLY
                     PKGLVSPIQECQFTTLGKNITALEIDGTFDDCQRLVKNAFADKELNSSLKLTSANSIN
                     VARFLPQAFYYFYAYAQLKAIHPKFTQEELEMVICVPSGNFGNITAGLFAKEMGLPVK
                     RFIAANNRNDIFLQYLKTGKYQPRPSVQTIANAMDVGDPSNFARILDLYHGSHDKISS
                     LISGVSYTDEEISATIKECYRETGYLLDPHGACGYRALRENLTNNENGIFLETAHPAK
                     FLDTVEKIIGEKVEIPAKLQEFMKGEKQSIPLKNDFESFKNFLLSAALGNKD"
     gene            complement(1392..1838)
                     /locus_tag="PLZ54_07595"
     CDS             complement(1392..1838)
                     /locus_tag="PLZ54_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOJ66825.1"
                     /translation="MLEFTELGYLGLFIGSFLAATVVPFSSEVIFSTMVFGGLNAWTC
                     VFVATAGNWLGGMTSFYLGKLGKIEWIEKYFHVKKEKIDQFLEKFHKYGEWFVFFSFL
                     PFIGDAIAIAAGFLRARWWATAAFMLAGKFCRYVVWMYVNGLILMI"
     gene            complement(1845..2840)
                     /locus_tag="PLZ54_07600"
     CDS             complement(1845..2840)
                     /locus_tag="PLZ54_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66826.1"
                     /translation="MGRRQISIKSLITPTIPGTVLLVLFCVGWWYFPYLIGKQPFMLH
                     DDPPFVLSVFSFNPAINNFLSLLLTVLNAILIQNLNLRFNILRKRSLLPFLIFLFLMA
                     GWYPLHFIWELHLALSIFILAFYYFFDMYRQPAAVEQAFMGSFLIAVASLFLPPLLWM
                     VPVVWINFLQMRAFTLRTFLASILGILSPWILYAGGYFVFQDNFDFNKLIFSRFFDGG
                     KYDFSSFQSIDLIYFVSLAGILLISIVYIYMNFQQDSISTRSKLNMIIIQMLFLLGIS
                     IFHASVFYCFLPFAVFFYVLLLSHPFSTRENNFFSILFLVFCLLNVAFVLFKIIA"
     gene            complement(2874..4970)
                     /locus_tag="PLZ54_07605"
     CDS             complement(2874..4970)
                     /locus_tag="PLZ54_07605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOJ66827.1"
                     /translation="MKKLIVAVMIGTLLTGCSTNKDNPFLQAYDTPFSVPPFDKIKTE
                     HYLPAFREGIKTHQKEIDAIAENSEAPTFANTVEALDFSGELLKKVSSVFFNLYSTDT
                     NDKMEAIANEVTPLLTEHSDNLYLNKALFQRIKTLYDKRSTLGLNAEQNRLLEKYYKN
                     FVRNGAALNDEQKEKLREINKQLSLAELQFGQNVLAETNAYQKWITDEKELVGLPENV
                     KQSAAEAAKEAGREGQWLFTTKKASFIPVLQYSENRELRRELLTAYNKRADNGNKNDN
                     KPVINKIVKLRVQKAQLLGYPTWADYKLDDTMAKTPQKVYEFLASVWEPSVRKAREEA
                     AELQKMMDAEGKGEKLEPWDWWFYAEKLRKAKYDLEEEALKPYFKLENVRKGVFDLAS
                     KLYGLKFKKLENIPVYHPDVEVFEVTDANDSLIGILYTDYYPRAGKQAGAWMNNISEQ
                     YIKDNINHRPVICNVGNLTKPTSDKPALLSMDDVETLFHEFGHALHGLLSQCTYPSLS
                     GTNVARDFVELPSQIMENWCWEPEVIKTYAFHYKTGEVMPNELIEKIQQASTFNQGFA
                     MTELLSAALLDMDYHTIKDTADIDVTAFENATMKRIGMIPQIVVRYRSTYFNHVFSGG
                     YSAGYYSYTWAEVLDADAFQAFKETGDIYNSEIAASFRKNILERGDSDDPMKLYVAFR
                     GKEPQPDALLIKRGLK"
     gene            complement(5115..6041)
                     /locus_tag="PLZ54_07610"
     CDS             complement(5115..6041)
                     /locus_tag="PLZ54_07610"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOJ66828.1"
                     /translation="MQVIEANSGIKIEPCVATVGFFDGVHIGHRFLMEELKKIASTQH
                     LKSVVVTFDQHPRKVLNADFQPKLLTTLDERLFQIGSTGVDACVVLNFTREMSLLSAR
                     DFLKEVLHDQLNVQTLLVGHDHRFGHNREDGLSEYIRYGEEFGIKVIEAQRFATEDMP
                     HISSSVIRRALLDGDVETANRLLMYEYSFEGKVVNGFKVGHKIGFPTANLIPLSEEKL
                     IPGVGVYAVRARCNEKKFKAMMDIGYRPTFGDDNKLTIEVHIIDFDEDIYQKNIRIDF
                     IRRLRDEIKFDTVEELIEQLYRDREEVLAMNF"
     gene            complement(6110..>6312)
                     /locus_tag="PLZ54_07615"
     CDS             complement(6110..>6312)
                     /locus_tag="PLZ54_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOJ66829.1"
                     /translation="LGITKPDIRVFNSVIEDIGLPPQQCLFLDDGLQNIEQAQKTGLQ
                     TYWVKQQEDLSFLLQPDVFVFD"
BASE COUNT         2017 a         1338 c         1136 g         1821 t
ORIGIN      
        1 cagacatgtg ggttccatca gaccattaaa aaataaatta tattctgatg aaatactaca
       61 gcaccaacaa aggaatttcg ggtgttagtc tgaaagatgc cgttatcaaa ggcttggccg
      121 acgacaaagg acttttcatg ccggagcaaa tcaagcagtt gcctccttct tttttcgata
      181 ccatcatcaa tatgtccttc caagaaatag cattggcagt ggcagaagca ttttttggag
      241 aagatgtaga taacgaagca ctgaaaaata ttgtgtacga aaccttgtcg tttgatgttc
      301 cgttggtgca tgttaaagac aatatcttca gtctggaact ttttcatggg ccaacgctgg
      361 cgtttaaaga cgttggtgcc agattcatgt cccgtttgct ccgctatttt atccagaaag
      421 aaggcgaaaa gaacgtaaat gtattggttg ccacttccgg cgacactgga agcgctgttg
      481 ccaacggttt tttaggcgta gaaggcatac acgtttttgt cctttacccg aaaggattgg
      541 tcagtcccat tcaggaatgt cagtttacca ctttaggaaa aaatattacc gcgctggaaa
      601 tagatggcac attcgacgac tgtcaacgtc tggttaaaaa tgcttttgca gataaggaat
      661 tgaattcttc cctaaaactt acttcggcaa actctattaa tgttgcccga ttccttcctc
      721 aggcttttta ttatttttat gcttatgcac aattaaaagc cattcatcca aagttcacac
      781 aggaagaatt ggaaatggtt atttgcgtac caagtggaaa ttttgggaat attactgccg
      841 gcctttttgc caaagagatg ggattgcctg tgaaacgctt tattgctgcc aacaaccgca
      901 acgatatttt tcttcaatat ctgaaaaccg gtaaatacca accacgtcct tccgtacaaa
      961 ccattgcaaa cgctatggat gtaggcgacc caagcaattt tgcccgtatt ctcgatttat
     1021 atcatggttc gcatgataaa atcagttctt tgatttcggg tgtcagctat accgacgaag
     1081 aaatttctgc caccattaag gaatgttatc gtgaaaccgg ttatttgctt gatcctcatg
     1141 gagcttgtgg ctatagagct ttgcgcgaaa acttgacaaa taacgaaaac ggaatttttc
     1201 tggaaacagc tcatccagct aaatttttgg atacggtaga aaaaataatt ggagaaaaag
     1261 ttgaaattcc tgccaaactt caagaattta tgaaaggcga aaagcaaagc attcctttaa
     1321 aaaacgattt cgaatcgttc aaaaactttc ttttatcggc tgctttgggg aataaggatt
     1381 gacaacattt gctaaatcat taatatcaaa ccgttcacat acatccaaac gacgtaacgg
     1441 caaaatttcc cggcaagcat aaaagctgcc gttgcccacc agcgggcacg taaaaaaccg
     1501 gctgcaatag ctatggcatc gccaatgaaa ggcaggaaag agaaaaacac aaaccattct
     1561 ccatatttgt gaaatttttc cagaaattgg tcaatttttt cttttttgac atggaaatat
     1621 ttttctatcc attctatttt ccccaatttc cccaaataaa aagatgtcat tcctccaagc
     1681 cagttgccag ctgtagccac aaaaacacaa gtccaagcat tcaaaccacc aaaaaccatt
     1741 gtggaaaata taacttccga actgaaagga accaccgttg ctgcaagaaa tgaccctata
     1801 aaaagtccca aataacccaa ttccgtaaat tccaacatga gtggttaggc aattatttta
     1861 aacaacacaa aggccacatt tagcaagcaa aaaacaagga acaaaatgct gaaaaaatta
     1921 ttttctctcg tagaaaaagg atgagacaaa agcaatacat agaaaaaaac cgcgaaggga
     1981 aggaaacagt aaaagaccga agcatgaaaa atggaaattc ccaacaaaaa caacatttga
     2041 ataatgatca tattcaactt cgaccgcgtg ctaatgctat cctgctgaaa attcatgtag
     2101 atataaacta tcgaaatcaa taaaatcccg gccagtgaaa caaaataaat caaatcaatg
     2161 gattgaaatg aagaaaaatc atattttcct ccatcaaaaa aacgactgaa aatcaatttg
     2221 ttgaagtcaa aattatcctg aaaaacaaaa tacccacctg cataaagtat ccacggactc
     2281 aatatcccca aaatggaagc aagaaaagtt ctcagcgtga aagctcgcat ttgtaaaaaa
     2341 ttaatccaaa ccaccggcac catccagagc aaaggtggca aaaacagact tgcaacagct
     2401 atcagaaagc tacccatgaa agcctgctca acagcagccg gctgacgata catgtcgaag
     2461 aaataataaa aagccagaat aaaaatgctc aatgccaaat gaagctccca aataaaatgc
     2521 aacggatacc atccggccat taaaaacaaa aaaatcaaaa aaggcaataa agaccttttt
     2581 ctgagaatat tgaagcgtag attcaaattc tgaatcaata tcgcattcaa tacagttaaa
     2641 agcaaactta aaaaattgtt aatagccgga ttgaatgaaa aaacagaaag aacaaacgga
     2701 gggtcatcgt gaagcataaa tggctgcttc ccaatcaaat aggggaaata ccaccagcct
     2761 acacagaaca gtacaagcag cactgttccg ggtatagtag gagttattaa agatttaatg
     2821 gatatttgac gcctgcccac tgaaagtttt tttaaaaatt ttcctcttca acattatttt
     2881 aatcctcttt ttatcaaaag agcatcgggt tgaggttcct taccgcgaaa agccacatac
     2941 aacttcatgg gatcatccga atcgccccgc tcaagaatat tcttacgaaa agaagcagct
     3001 atttcggaat tatatatatc gccagtttcc ttaaaagcct gaaaagcgtc ggcatcgagt
     3061 acttcagccc atgtatagct atagtatccg gcagaatatc ctcccgaaaa aacatggttg
     3121 aaataggtac tccgataacg caccactatt tgaggaatca tgccgatgcg cttcatggtg
     3181 gcattttcaa aagcggtaac atctatatcg gcggtatctt taatggtatg ataatccata
     3241 tcgagcaatg ctgccgaaag cagttccgtc atggcaaacc cttgattgaa agtacttgcc
     3301 tgctgaattt tctctatcag ttcattgggc atcacttctc cggttttata atgaaaagca
     3361 taagttttta ttacttccgg ttcccaacac cagttttcca taatttgtga aggaagttct
     3421 acaaaatcac gagctacgtt tgtacccgag agagaaggat aagtgcactg agaaagtaaa
     3481 ccgtgcaaag catgtccaaa ctcatgaaat aaagtctcca cgtcgtccat gctcaacaaa
     3541 gcaggtttgt cggaagtggg cttggtcaga ttgccaacat tacaaataac gggtcgatga
     3601 ttgatattat ccttgatgta ttgttccgaa atgttgttca tccatgcgcc tgcctgcttg
     3661 ccggcacgag gatagtaatc ggtatagaga atgccgatca gcgaatcgtt ggcatcggtt
     3721 acttcaaaaa cttcgacatc gggatggtaa accggtatat tttcgagttt tttaaacttc
     3781 aatccgtaaa gtttggaagc taaatcaaaa accccttttc tgacattctc gagtttaaaa
     3841 tatggtttta atgcttcttc ttccaaatcg tatttggctt ttcgaagttt ttcggcataa
     3901 aaccaccaat cccacggttc caatttttct ccttttcctt cggcatccat cattttttgc
     3961 aattctgccg cttcttctcg tgctttacgt acggaaggtt cccacacgga agccaaaaat
     4021 tcataaactt tttgaggtgt tttggccatt gtatcgtcca atttataatc ggcccaagtg
     4081 ggataaccca ataattgggc tttttgcacg cggagtttca caattttatt gatcaccggt
     4141 ttgttgtcgt ttttattgcc attatcggca cgcttgttat aagcggtcaa taattctcga
     4201 cgcaattcac gattttcgct atattgaaga acgggaatga aactcgcctt ttttgtggtg
     4261 aaaagccatt gtccttctct cccagcctct ttggcagcct cagctgccga ctgtttgaca
     4321 ttttccggca atccgaccaa ttctttttcg tcagtaatcc atttttgata agcatttgtc
     4381 tctgccaata cgttttggcc aaactgcaac tctgccaaac taagctgttt gttgatttct
     4441 ctcaattttt ccttttgctc atcgtttaat gcagcaccat tgcgaacaaa atttttgtaa
     4501 tacttctcca gcaacctgtt ttgttcggca ttgagtccaa gtgtggaacg tttatcgtaa
     4561 agggttttaa ttcgttgaaa cagcgcctta ttgagataaa gattatcact gtgttccgtc
     4621 agaagcggcg ttacctcgtt ggcaatggct tccattttat cgttggtatc ggtagaatag
     4681 agattgaaaa atacggaaga aacttttttc agcaattctc cactgaaatc caatgcttca
     4741 accgtattgg caaaagttgg cgcttcgcta ttctcggcaa tggcgtcaat ttccttctga
     4801 tgagttttta ttccttcacg aaaggcagga agataatgtt cagtcttgat tttatcaaat
     4861 ggaggaacgc taaaaggagt atcataagcc tgcaagaaag gattgtcctt gttggtagaa
     4921 caaccggtta ataatgtacc aatcataacg gcaacaatta attttttcat aaataaatga
     4981 ttttaacaaa tttgtcgaaa aatattttcg gttaaataag atttttgatt atcaattgct
     5041 atgttttata cagcaaaacg aaaaattgtt tgctttcctt tcaaaaatac aacgaaagca
     5101 tgaaaaatga aatttcagaa attcattgcc aaaacttcct ccctgtcccg atacaactgc
     5161 tcaatcagtt cttctaccgt atcgaattta atttcatctc tcagccgacg gataaagtca
     5221 attctaatgt ttttttgata aatatcttca tcgaaatcga taatgtgaac ctcaatcgtc
     5281 aacttgttgt catccccgaa tgtaggtcga taacctatgt ccatcatggc tttgaatttt
     5341 ttttcgttac aacgcgctct aacggcatac actccaacac cgggaattaa tttttcttca
     5401 cttaacggaa tcaggtttgc agtggggaat ccaattttat gacctacctt aaagccattt
     5461 acaacctttc cttcaaaaga atattcatac atcaacaatc gatttgccgt ttccacatct
     5521 ccatcgagta gtgctcgacg aataacggaa gaactgatat gaggcatatc ttctgtagca
     5581 aaccgctgag cttctataac ttttattcca aattcttctc cgtagcggat atactccgac
     5641 aatccatctt cccgattatg accaaaacga tggtcgtgcc ccaccagcag agtctgtaca
     5701 ttgagttgat cgtgaagaac ctcttttaaa aaatcccgcg cggacaacaa agacatttca
     5761 cgcgtaaaat tcagcaccac acaagcatca acacctgtcg aaccgatttg gaaaagtctt
     5821 tcgtccaacg tagtgagcaa ttttggctga aaatcggcat ttaaaacttt acgcggatgc
     5881 tggtcgaaag ttacaaccac gcttttcaaa tgttgcgtcg aagctatctt tttcaattct
     5941 tccattaaaa atcgatgacc aatatgcacg ccatcaaaaa aacctactgt ggcaacacaa
     6001 ggttctattt ttattccgct gttcgcttct atcacttgca tgaaattccc actaaaatga
     6061 aatacattga aaatttattt ggaaaaacct attgattttt ctacttgttt taatcaaaaa
     6121 caaaaacatc gggttgaagc aggaaactca aatcttcctg ttgtttcacc caataagttt
     6181 gcaagccggt tttttgtgcc tgttcaatat tttgcaaccc atcatccagg aaaaggcatt
     6241 gctgcggtgg tagcccaata tcctctatca cggaattaaa cacacggatg tcgggcttgg
     6301 ttattcccaa ct
//
LOCUS       DAOSCH010000107         6306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_7884_length_6306_cov_6.175652, whole genome shotgun sequence.
ACCESSION   DAOSCH010000107 DAOSCH010000000
VERSION     DAOSCH010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7884_length_6306_cov_6.175652"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..847
                     /gene="coaE"
                     /locus_tag="PLZ54_07620"
     CDS             245..847
                     /gene="coaE"
                     /locus_tag="PLZ54_07620"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOJ66830.1"
                     /translation="MNRPLIVGITGGIGSGKSTLSNKLRAEGYQVYDCDKEAKKLQNE
                     HPVIREKIRELFGEDIYTESGLDRKKVADIVFQNRDLLDQLNKIVHPFVREDFLKWVG
                     QYITEKFLFMESAILFESGFIDMVDKVILMTASEDVRIRRVVKRDGIDPERVRARISK
                     QLPDTEKIPFSDFIIHSDDGKPLYDKMKKILKDMENYVLD"
     gene            complement(1129..2058)
                     /locus_tag="PLZ54_07625"
     CDS             complement(1129..2058)
                     /locus_tag="PLZ54_07625"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
                     /protein_id="HOJ66831.1"
                     /translation="MLNKTFLFILMVLQIITEPLENKSIESEIIPSQSEKLRILSWNI
                     YMLPVVSLFNGNTKRAKLIAEKLHNSDYQIIVFQEAFSSASRSIISKQLKDTYPYQYG
                     PANKKHPPLKTNSGLWVVSKIPLEELDEIRYNVSEGYDKIARKGAAIFQGQFNGVEFQ
                     LITTHLQADAAPEIREKQCIEIMDKLLNKYYQPHIPQIICGDFNIEMDNKKDYPVMLQ
                     TLDATNGEITGDKTTYDEVFNTLGRKREGKRKTIDYVLIRNEQWIKEIKRRVNTFYEE
                     SSRYIGHLSDHYALEAELVFVESNVNQMSISHR"
     gene            complement(2271..3470)
                     /gene="argG"
                     /locus_tag="PLZ54_07630"
     CDS             complement(2271..3470)
                     /gene="argG"
                     /locus_tag="PLZ54_07630"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HOJ66832.1"
                     /translation="MKEKVVLAFSGGLDTSFCVKYLSEEKGFDVYTAVANTGGFSDAD
                     LQNIEKRAYQLGAVKHVVLDVTKDYYEKCIRYMVFGNVLRNGTYPISVSSERTFQAIA
                     IINYAKEIGADYVAHGSTGAGNDQVRFDLTFSVLAPEIKILTPIRDLALSREYEINYL
                     RQHGFEADFKKMEYSINKGLWGTSIGGKETLKSDQTLPEEAYPSKVEKQGEEILTIEF
                     EEGEICGVNGQKNPDKIALINLVEKIGSQYGIGRDMHVGDTIIGIKGRVAFEAAAPML
                     IINAHKLLEKHVLTKWQQYWKEQLGNWYGMFLHEAQYLEPVMRNIEKFLESSQQNVTG
                     TVILKLRPYCFTLVGVDSPFDMMKTDMGEYGEVNKGWTADDVKGFTKILSNQLKIYYT
                     LQNKNQQ"
     gene            complement(3564..4160)
                     /locus_tag="PLZ54_07635"
     CDS             complement(3564..4160)
                     /locus_tag="PLZ54_07635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ66833.1"
                     /translation="MENNFSMDEITVQVADESYIKYVDTILKTIEEAAKIRGTGIARR
                     SPEYIEQKMKEGKAIIALHGEEFAGFCYIETWSDKKFVANSGLIVVEKFRGHGLAKRI
                     KQKAFELSRERYPDAKIFGLTTGLAVMKINSELGYKPVTFSELTNDEAFWAGCQSCVN
                     YDILQRTGRKYCLCTGMLFDPAAEQKKSSTPTSEKEEK"
     gene            complement(4231..4713)
                     /locus_tag="PLZ54_07640"
     CDS             complement(4231..4713)
                     /locus_tag="PLZ54_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HOJ66834.1"
                     /translation="MKNKRNRLKIISDILRSNVVGSQEELLKLLKEQQCEITQATLSR
                     DLKVLKVAKTPLSNGTYKYVLPSYNKPLPPPSANTLNGSLANGVILSLEFSQQLAVLK
                     TKPGYASAIAWDIDNKASDYILGTIAGDDTILVVPREGVEREVVVEIMNDIFGEPKAV
                     "
     gene            5187..>6306
                     /gene="coaBC"
                     /locus_tag="PLZ54_07645"
     CDS             5187..>6306
                     /gene="coaBC"
                     /locus_tag="PLZ54_07645"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOJ66835.1"
                     /translation="MLQDKKIILGITGGIAAYKSATIVRLLVKQGAEVKVVMSASAKE
                     FITPLTLAALSKNPVLTDFFDPTNGQWNSHVDLGLWADAYLIAPATANSLAKMAAGIA
                     DNLLLTTYLSAKCPVFLAPAMDLDMFAHPATQRNLQILRSYGNLVIEPSFGELASGLE
                     GKGRMEEPENIVHFLDDYFTPKQLAGKKILITAGPTYEKIDPVRFIGNFSSGKMGFAL
                     AESCAAHGADVCLIAGPVQLSLQHKNIQLIKVSSAQEMFDAVTEKFPEMDGTILSAAV
                     ADFTPVAIAEDKIKRKGDRLTIELKPTPDIAATLGKIKTEKQFLVGFALETSNEIENA
                     QLKLKNKNLDFIVLNSLRDEKAGFGYDTNKITILHNTGK"
BASE COUNT         1886 a         1189 c         1113 g         2118 t
ORIGIN      
        1 ccttttattt cccctgaaaa agttgcatta attacttgaa tttacgtata aaaaacaaat
       61 caaagtttct tttttgcatg gtcaatattc agaagtagtt tttctcaaaa attttgccta
      121 tatttgcata actctatttc taaatccaat cattcttgtt ggtaaaatca tttatcgttt
      181 tttttttgaa taaaggaatt tttttccttg caagtcctat tagtttatta ttaagtcttt
      241 ttttatgaat cgtccgctta tcgtaggcat tacaggaggt attggaagtg gaaaatcgac
      301 gctttccaac aagttgcgtg ctgaaggtta tcaggtgtat gattgtgaca aggaggccaa
      361 aaaactacaa aatgaacatc cggtaattcg agaaaaaatt agagaattat ttggagaaga
      421 tatttacacc gagagtggat tagatcgcaa aaaagttgcc gatattgttt ttcagaaccg
      481 tgatttactc gatcaactga acaaaattgt ccaccctttt gtcagagaag attttttaaa
      541 atgggtcgga caatacataa cagaaaaatt tctgtttatg gaatctgcca ttttgttcga
      601 aagtggattt attgatatgg ttgacaaggt gatattaatg acagcatccg aagatgtaag
      661 aataagaagg gtggtaaaac gtgatggaat tgatccggaa agggtcagag ctcgcatctc
      721 aaaacaattg cccgataccg aaaaaattcc cttttccgat tttatcatac attccgacga
      781 tgggaaacct ctgtacgata aaatgaaaaa aattttaaaa gatatggaaa actatgtatt
      841 ggattgattc gaaaggggaa aattaaactt ttatgcaata atcgttttct gtttgttcca
      901 ttaaattaaa taaaagtttt ctgtcgttaa attttctacg caggcaaatt tattttttat
      961 atttggatgg ccatattctg caacttccta tatctgcttt tattagcggt tcttgcgggt
     1021 aacaaattca ctctccttta ttttttgtaa ctgaagaagt tcctctgtat tttttgtttg
     1081 taaaagacac aatgcaatcg ggtgggagcg taggtattaa gaaaatattt accgatggct
     1141 aatagacatt tgattcacat tgctttcaac aaagacgagt tcggcttcca gtgcataatg
     1201 atcagaaagg tgtccaatat agcggctact ttcttcataa aatgtattta cgcgacgttt
     1261 aatttctttt atccattgtt cgtttcgaat caatacatag tcaatcgttt tccttttacc
     1321 ttctcttttt cgacccaatg tattgaaaac ctcgtcataa gtagtcttgt ctccggttat
     1381 ctcaccattg gttgcatcaa gggtttgtaa cattactgga taatcttttt tattgtccat
     1441 ttcaatattg aaatcaccgc agataatttg aggaatatgg ggttggtaat atttattcaa
     1501 taatttatcc ataatttcaa tacattgttt ttcacgaatt tccggagctg catcagcttg
     1561 caggtgcgtg gttattaatt gaaattctac accgttaaat tgtccctgaa aaatggccgc
     1621 cccttttctt gcaattttat cataaccttc gctaacattg tatctaattt catcaagctc
     1681 ttccaacgga attttactta ccacccaaag accactgttc gttttcaatg gaggatgttt
     1741 tttatttgcc gggccatatt gataaggata agtatctttt aattgtttgg atataatgct
     1801 tctggatgca ctactgaatg cttcctgaaa tacaataatc tgataatcag aattatgtag
     1861 tttttcagca attagtttcg ctctcttggt atttccattg aaaaggctaa cgaccggaag
     1921 catatagata ttccaactta atatcctcaa tttttccgac tgactaggga taatctcact
     1981 ttcaatagat ttattttcaa gtggttcagt aattatctgt aataccatta aaataaaaag
     2041 gaatgtttta ttaagcataa aggttggttt ttaattttta tatggcaaaa gaaatgtaat
     2101 gctgtttcat aattgttaaa taccaataac ttataagtga agacgaaatg aatgttccat
     2161 gaagaaaata ggtggatagc ttgttaaaaa caagaggaat ggaataaaaa aacctctgcc
     2221 gaagaagatc ccgacagagg ttaaaaaatt ttaatttcag tacgaaaatt ttactgctga
     2281 tttttgttct gcaatgtgta atagattttc aactgattgg aaagtatttt ggtaaatccc
     2341 tttacatcgt cggctgtcca gcctttattc acttcgccat attctcccat atcggttttc
     2401 atcatgtcga aaggactgtc aacgcctacc aaagtaaagc aataaggccg aagtttcaaa
     2461 ataacggttc ccgtaacgtt ttgctgtgaa ctttcaagaa atttttcaat gttgcgcatg
     2521 accggttcga gatattgggc ttcgtgtaaa aacatgccgt accagtttcc caactgctct
     2581 ttccagtatt gctgccattt ggtcaacaca tgtttttcga gcaatttatg agcattgata
     2641 ataagcatgg gtgctgcagc ttcaaaagct acccgtcctt tgatgccgat aatggtatcg
     2701 cccacatgca tatcgcgacc aatgccgtat tgagaaccaa ttttttcaac cagattgatc
     2761 aatgcaattt tatcgggatt tttttggcca ttcactccgc aaatttctcc ctcttcaaat
     2821 tcgatagtga gaatttcttc gccctgtttt tccactttgc tcggataagc ttcttccggc
     2881 agcgtttgat ccgatttcag agtctccttt ccgccaatgc ttgtgcccca caatcctttg
     2941 ttgatggaat attccatttt tttgaaatcg gcttcaaatc cgtgttgtct taaataattg
     3001 atttcgtatt cgcgactcaa ggcaagatca cgaataggtg tcagaatttt tatttccggt
     3061 gccagtacac tgaaagtcaa atcgaaacga acctgatcgt tgccggctcc tgtactgcca
     3121 tgtgccacgt aatcggctcc aatttctttg gcataattaa ttatggctat ggcctgaaaa
     3181 gtacgctccg aactcacaga aattggatac gtaccatttc ttaacacatt gccgaaaacc
     3241 atgtatcgaa tacatttttc gtaataatct ttggttacat cgagcaccac atgtttcact
     3301 gctcccagct gataagctct cttctcgatg ttttgcaaat cggcatcact aaatccgcct
     3361 gtattggcaa cagcggtata tacatcaaat cctttttctt ccgaaagata ctttacgcaa
     3421 aacgacgtat ccaatccacc actaaaggct aaaacaactt tttctttcat ttttctttga
     3481 atatcttatt tttctattga ttttctgaaa tttttctttt ttaaacggga tttaaaaccc
     3541 atttaattcg attttaaaag ggattatttt tcttcttttt ccgatgtcgg ggtcgaagat
     3601 tttttttgtt cagcagcagg gtcaaacaac atgccggtac aaaggcaata ttttcgtccg
     3661 gttcgttgca gaatatcata gtttacacaa ctttggcacc cggcccaaaa agcttcatca
     3721 ttggtaagtt cagagaaagt taccggtttg taacccaatt cggaatttat tttcatcaca
     3781 gccaatcctg tagtaagacc aaaaatcttg gcgtcaggat aacgttcgcg cgaaagttca
     3841 aaagctttct gcttaattct tttggccaat ccatgtcctc tgaatttttc taccacaatc
     3901 aaaccggaat tggctacaaa ttttttgtcg ctccaagttt caatgtagca aaaaccggca
     3961 aactcttctc catgaagagc aataatggct tttccttcct tcattttttg ctcaatatat
     4021 tcgggcgaac gacgtgcaat gcctgttcct ctgatttttg ctgcttcttc gatggttttc
     4081 aatatggtat ccacatattt gatgtaactt tcatcggcaa cctgaacggt aatctcatcc
     4141 atgctaaagt tattttccat aatttttttg gattcctcct atcttcttca tttataaatt
     4201 tttatctacg aatttcatct atcaaaaaaa tcaaactgct tttggctcgc caaaaatatc
     4261 gttcataatc tctacgacta cttcccgctc cactccttca cgcggcacta ccaaaatggt
     4321 atcgtcgccg gctatggttc ccaaaatgta atccgatgct ttgttgtcaa tatcccaagc
     4381 tatcgcgctg gcatatcccg gtttcgtctt caacacggcc agttgctgcg aaaattccaa
     4441 acttagaatc actccattgg ccaacgaacc attcagcgta ttggccgaag gtggtggcag
     4501 gggcttgttg tacgaaggca acacgtattt gtatgtccca ttggaaagcg gagttttggc
     4561 cactttcaac actttcaggt cgcgcgacag agttgcttgc gtaatttcgc actgctgttc
     4621 cttcagtaat ttcaacaatt cttcctggct gccaaccaca tttgaacgaa gaatgtcgga
     4681 aataattttt aagcggtttc gtttgttttt cataaaattt taatatataa gtatttgttt
     4741 gtaattactt ttctgatgaa acccacatgt atttcataca caaacacaaa tgaatataat
     4801 attcagacat gtgggttcca tcagaccttt aaaaaataaa ttatattctg atgaaaacga
     4861 atgtcttata tgaaaccaaa ctgattctct actcaaatat ttttagtata tttataagaa
     4921 tattttgcaa atatacgaaa tttattccaa aatcacgaat aaataactaa taaatatcct
     4981 tattaatgcc attatttaac aattattcga ttaaacaggt atatttatgc aggaaaaatc
     5041 taaataatta ctcaaaacat aaaagaatat taaatgaata aaaagtgggt aacactatca
     5101 gtattatgtt ttttgaagta ttacaggaaa attgaaaatt atcttgtaaa tttgcaggat
     5161 ataattcatc cataataaag gttgatatgc tgcaggataa aaaaatcatt ttgggcatta
     5221 cgggtggaat tgctgcttat aagtctgcca cgatagttcg tttactggtg aagcagggag
     5281 ctgaagtaaa agtggttatg tcggcttcgg caaaagaatt tattacaccc ttgacacttg
     5341 ctgcactttc caaaaatccg gtattgaccg attttttcga tccaacaaat ggtcaatgga
     5401 acagccatgt ggatttgggc ttgtgggctg atgcatattt aattgcaccg gcaacagcca
     5461 attcattggc taaaatggca gccggcattg ctgataattt gcttttgact acttatcttt
     5521 cggccaagtg tcctgttttt ttagctccag caatggattt ggatatgttt gctcatcctg
     5581 ctacacagcg caacttgcaa attcttcgtt cgtatggaaa cctcgttata gaaccttcat
     5641 ttggtgaatt ggcaagtggt ttggaaggga aggggagaat ggaagaaccc gaaaatattg
     5701 ttcattttct tgacgattat tttacaccca aacaacttgc ggggaaaaaa attctgatta
     5761 ctgccggtcc tacctatgaa aaaatagatc cggttcgttt tattggaaat ttctcatccg
     5821 gaaaaatggg ttttgcgttg gctgaatcat gtgctgctca tggagctgat gtttgtctga
     5881 ttgcagggcc tgttcaatta agtttgcaac ataaaaatat tcaactcatt aaagtttcat
     5941 ccgctcagga aatgttcgac gctgttacgg aaaagtttcc cgaaatggat ggtaccatat
     6001 taagtgctgc agtagcagat tttacaccgg tggctattgc cgaagacaag ataaaacgta
     6061 agggcgatcg gttgaccata gaactaaaac cgactccgga tattgctgct acactcggca
     6121 aaataaaaac cgaaaaacaa tttttagtag gttttgctct cgaaacttcc aatgaaattg
     6181 aaaatgcaca attgaagttg aaaaataaaa atctggattt tattgtactc aattctcttc
     6241 gtgacgaaaa agccggattt ggctacgata ccaataaaat taccattttg cataataccg
     6301 gcaaaa
//
LOCUS       DAOSCH010000108         6303 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_7887_length_6303_cov_5.897887, whole genome shotgun sequence.
ACCESSION   DAOSCH010000108 DAOSCH010000000
VERSION     DAOSCH010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7887_length_6303_cov_5.897887"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLZ54_07650"
     CDS             complement(<1..184)
                     /locus_tag="PLZ54_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOJ66836.1"
                     /translation="MKINNFLFSVYQWIICVPIVIVLTLLTSIFTIILSPIFPNSNIS
                     YLPARWWSKATCGLFFI"
     gene            complement(363..1256)
                     /locus_tag="PLZ54_07655"
     CDS             complement(363..1256)
                     /locus_tag="PLZ54_07655"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOJ66837.1"
                     /translation="MSRFKSLSLYASIVAAMLIWAFSFIWTKEALTSFRPMLLITCRL
                     IIASVLMLIVSILSGRFQKIQKQDLKWFLLLALFEPYLYYIGETFGLTMVSSTLASVI
                     ISTIPVFSPLLAFLFLKERIGWFNVAGIILSLTGVFLVIFEPSGNFHVQPLGIVLLFF
                     AVFSAVCYAVVLQKISIHYYTLNIILYQSLFGLMFFIPTTLLADYPYFSTLHFTLSSI
                     EALLMLSIFASVIAYVLFARVVRRIGVARSNVFTNLIPVFTAIFSWLLLNEQLTILKW
                     IGIATVVGGLFVSQARKIKFK"
     gene            1404..1477
                     /locus_tag="PLZ54_07660"
     tRNA            1404..1477
                     /locus_tag="PLZ54_07660"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1438..1440,aa:Asn,seq:gtt)
     gene            complement(1518..3056)
                     /locus_tag="PLZ54_07665"
     CDS             complement(1518..3056)
                     /locus_tag="PLZ54_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOJ66838.1"
                     /translation="MLVKTFGAAVQGINATIITIEVNVNQGIRFFLVGLPDNAVKESH
                     ERIASALDYIGYKMPRKQVVINMAPADIRKEGSAYDLPLAIGILAADGKINPDVLQNY
                     VIMGELSLDGSLQPIKGVLPISIKAREEKFKGFILPKQNAREAAVVNNLEVYGVENLK
                     EVIDFLNGKNQLEPTVVNTREEFFNSLNQSEVDFAEVKGQENVKRALEVAAAGGHNII
                     MIGPPGAGKSMLAKRIPTILPPLTLHEALETTKIHSVAGNIDSNTSLIAERPFRSPHH
                     TISDVALVGGGTFPQPGEISLAHNGVLFLDELPEFKRTVLEVLRQPLEDRKICISRAK
                     FTIEYPASFMLVAAMNPCPCGYYNHPEKECVCAPGVVQKYLNRISGPLLDRIDIHIEI
                     VPVPFEKLSEKRDAESSAQIRERVIKARQIQELRFRDIDGVYCNAQMTTKLQRMFAVP
                     DKEGLELLKNAMQRLNLSARAYDRILKVSRTIADLDDSEKILPYHIAEAINYRNLDRE
                     GWAG"
     gene            complement(3111..4145)
                     /locus_tag="PLZ54_07670"
     CDS             complement(3111..4145)
                     /locus_tag="PLZ54_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66839.1"
                     /translation="MVKWIRGFFLLGIFIPFSLQSQVLKGELGLNYFFDNTEFSSSTL
                     TIDQTMKGVHLIPGVAVEWDKNNSLHGGTDLLNISGSQKTIEKIDLVAYYQFQSRKTL
                     FRAGSFPRQNILSNYSDFFFKDSIRYFRPNLQGLFWQVSNPTSFFNLWLDWTGHQTAT
                     QRESFFIGASANKRQHLFFADFQSYMFHYANTAPRNPEYHVCDNLLAHLSAGVDLTSL
                     TQLDTLMFAVGILEGFERERGMDNMTFTPSGLVVRNEIEYGKVGVSNTLYWGDARMKL
                     YEKHGWNLYWSNPFFRSGSYFESKWYWNMLKSRQVNGKLAMEFHVSEGKVFFEQLFTL
                     SASVDKLLKK"
     gene            4662..6026
                     /gene="miaB"
                     /locus_tag="PLZ54_07675"
     CDS             4662..6026
                     /gene="miaB"
                     /locus_tag="PLZ54_07675"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOJ66840.1"
                     /translation="MGLKENTHTSVTENPHKKLYIETYGCQMNVADSEVVASIMQMDG
                     FVLTEDFSEADAVLINTCSIRDNAEQRVFERLKYFHSLKRKNKQLILGVIGCMAERMK
                     EELIEKQGVDLVAGPDAYLDLPNLMGAVEKGEKAINIQLSRTETYQEVIPARLGGQIS
                     GFISITRGCDNFCSYCIVPYTRGRERSRDVESILNELKDLQQKNFKEVTLLGQNVNSY
                     RVEQDGKIIGFPELLEIVAQAAPEMRFRFTTSHPKDMSDRTLETIARYPNLCRFIHLP
                     VQSGSNKILRLMNRKYTREWYLERIAAIRRILPDAAIGTDIFCGFHDETEEDHKQTLS
                     LMREVAFDMAFTFKYSERPGTYAARHLPDNVSEEVKLRRLNEIIALQNELSLESNLRD
                     VGKTFQVLVEGYSKRSKEQFFGRTSQNKVVIFPRQGRRIGETVKVKINSASSATLFGE
                     VEHL"
     gene            complement(6235..6303)
                     /locus_tag="PLZ54_07680"
     tRNA            complement(6235..6303)
                     /locus_tag="PLZ54_07680"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6272..6274),aa:Phe,seq:gaa)
BASE COUNT         2011 a         1162 c         1272 g         1858 t
ORIGIN      
        1 tgatgaagaa taatccgcag gttgctttcg accaccatcg tgccggcaaa taagatatat
       61 tgctgttcgg gaaaattgga gataaaatta tggtaaaaat agatgtcagc aaagttaaaa
      121 caattacaat gggaacacat ataatccatt gatatacaga gaataaaaaa ttattgattt
      181 tcatggattt gaaattattt ggaatagaaa aaaatcaatc aaagaatttt ataataaaac
      241 ttttttaaaa tttttcgaga ttaaatcaat agcaagaaaa gaaaattttt cggtgcaaag
      301 atatatgttt cccgtttatt tcaaaaagct tttagattaa acaaacaagt acggttgatt
      361 gtttatttga acttgatttt acgcgcctga ctaacaaaca gtccaccaac aacagtagca
      421 attcctatcc acttaagtat ggttaattgt tcgtttaata atagccagga gaaaatagcc
      481 gtaaaaaccg gtatcaaatt ggtaaaaaca ttgctgcgag ctactccgat tcttctcact
      541 acccttgcaa agagaacata agctattacc gaagcaaaaa tggacaacat aagcaatgct
      601 tcgatggaag atagggtaaa atgcaaagtt gagaaatagg gataatcagc cagcaatgta
      661 gtgggaataa aaaacatcaa tccgaaaagg ctttgataaa gaataatgtt cagcgtgtaa
      721 taatgaatgg aaattttttg caaaactacg gcataacaaa cggccgaaaa aacggcaaaa
      781 aagagtaaaa caatgccgag tggctggacg tggaaattgc cgcttggttc aaaaatgaca
      841 agaaaaacac cggtaagcga caaaattatt cctgctacat tgaaccagcc gatacgttct
      901 tttaaaaaca aaaaagccag tagcggcgaa aaaaccggaa tagttgaaat aataaccgat
      961 gccagtgtag aagaaaccat tgtcaaaccg aaagtttcgc caatgtaata gaggtatggt
     1021 tcaaacagag ccagtagcaa aaaccatttc aaatcttgtt tctgaatttt ctgaaagcga
     1081 cctgaaagta tcgaaacgat cagcattagt acagaagcta tgattaatcg acaggttatc
     1141 agcaacatgg gtcgaaatga agtcaacgct tcttttgtcc agataaacga gaaagcccag
     1201 ataagcatgg cagcaacaat ggaagcatat aaactcaatg atttgaacct tgacatatat
     1261 ttaagaatca gctcgttatt ttaatttatg tttttatttt ttctatcgac aaagaatttt
     1321 aaatcaaatt tcaatcggaa atttttgtgt atatcaaaaa agaatattac ttttgccttc
     1381 gctttttgaa agcaatatca aattcttcct tagctcagtc ggttagagca tctgactgtt
     1441 aatcagaggg tccttggttc aagtccaaga ggaagagcaa aaccaccgca aaggtggttt
     1501 ttttgtttgc tcagttttta tcctgcccat ccttcccggt cgaggtttct gtaattaatg
     1561 gcttcggcaa tgtgatacgg tagaattttc tccgaatcgt ccaagtcggc aattgttcgc
     1621 gaaactttta gaattctgtc gtaagcgcgt gccgatagat tgagtcgttg catggcattt
     1681 ttcaataatt ccagcccttc cttatcggga acggcaaaca tgcgttgcaa ttttgtggtc
     1741 atttgggcat tgcaataaac gccatcaata tccctgaacc gcagttcctg aatttgacga
     1801 gccttaatca ctcgttctct gatttgggcg ctactttcgg catctctttt ttcggaaagt
     1861 ttttcaaatg gcacaggaac aatttcgata tgaatgtcta ttctgtcgag cagaggccct
     1921 gaaatgcgat tcagatattt ttgtaccaca cccggagcgc aaacacattc tttttccgga
     1981 tggttgtaat agccgcaagg acagggattc atggctgcta ccagcataaa gctggcggga
     2041 tattctatgg taaatttggc tctcgaaata catattttcc tgtcttccaa tggttggcgc
     2101 aacacttcga gcactgttct tttaaattca ggcaattcgt ccagaaaaag aacaccgttg
     2161 tgggcaagcg aaatttctcc gggttgagga aatgtacctc cgcccaccag tgctacatcc
     2221 gaaatggtat gatgtggact tcggaacggg cgttcagcaa tgagcgaagt gttgctgtcg
     2281 atattgccgg caacggaatg aattttggtt gtttccaatg cttcatgaag agtcagaggt
     2341 ggaagaatag tggggattct tttggccagc atcgattttc ctgcgcccgg cggacctatc
     2401 atgattatat tgtgtccgcc agcagcagcc acttcgagcg ctcttttaac attttcctgc
     2461 cctttcactt cggcaaaatc tacttcggac tggttcaggc tgttgaaaaa ttcttctctc
     2521 gtatttacta ctgttggttc gagttgattt ttgccattca gaaaatcgat tacttccttt
     2581 aagttttcaa ctccgtaaac ttccaaatta tttactacgg cagcttcgcg agcattttgc
     2641 ttgggcagga taaatccctt gaatttttct tcgcgtgcct tgatggaaat aggcagaaca
     2701 ccttttatcg gttgcaaact tccatcaagc gacaattcgc ccataatgac gtaattttga
     2761 agcacatcgg gattaatttt cccatcggca gccaaaatgc cgatagccag cggtaagtcg
     2821 taagctgaac cttctttacg aatatcggcg ggggccatgt tgataaccac ttgtttgcga
     2881 ggcattttat agcctatata atccaatgcg gaagcaatgc gttcgtgact ttcttttact
     2941 gcattgtcgg gaagtcctac cagaaagaaa cgaattccct gatttacatt aacttcaatg
     3001 gtaataatgg tggcattaat gccttgaaca gcagctccaa aagtttttac cagcatgagg
     3061 aatattttta ggtgtaattc agataattaa ttttatttct gcagtatagc ttattttttt
     3121 aacagcttat ccaccgaagc cgaaagggtg aacaactgtt caaaaaatac ttttccttcc
     3181 gatacatgga attccatagc caattttccg ttcacttgcc tgcttttcag catgttccaa
     3241 taccatttgc tttcaaagta actgccggat cgaaaaaatg gattgctcca gtaaagattc
     3301 cagccatgtt tttcgtacaa tttcattctc gcatcgcccc aataaagcgt attggaaacg
     3361 ccaactttgc catattcaat ttcatttcga acaactaaac cggaaggcgt aaaagtcata
     3421 ttgtccatcc cacgttcgcg ctcaaaaccc tctaaaattc cgaccgcaaa cataagcgtg
     3481 tccaattgtg taagagaggt cagatcgact cctgcggaaa gatgggccag caaattgtcg
     3541 caaacatgat attcaggatt acgtggtgcc gtgttggcat aatgaaacat ataagattga
     3601 aaatcggcaa aaaacagatg ttgtcgtttg ttggcagacg ctccaataaa aaaagattcc
     3661 cgttgggtgg ctgtttgatg tcctgtccaa tcgagccaaa ggttaaaaaa tgaagtcgga
     3721 ttgctaactt gccagaaaag tccctgtaaa ttgggacgga aatatcgtat ggaatcttta
     3781 aaaaagaaat cggaatagtt ggaaagtatg ttttgacgcg gaaaagaacc ggcacggaaa
     3841 agagtttttc gtgattggaa ttgataataa gccaccaaat caattttttc tatggttttt
     3901 tgagaaccgg aaatgttcaa taaatcagtg cctccgtgta aggaattatt tttgtcccat
     3961 tccacagcca ctcctggaat taaatgaacg cctttcatgg tctgatcgat ggtaagggta
     4021 gaagaagaaa attcggtgtt gtcaaaaaaa tagtttaatc ccaattcgcc ttttaacact
     4081 tgactttgaa gggaaaaagg gatgaatatg ccaagcagaa agaaaccccg aatccattta
     4141 accataaaat ttttcaactt aaaattaatt ttaagcaaaa atacagatta tcttcatatt
     4201 ttggtgggtt ttagctgttt tttcttaaaa attgagtatg aacaacgaat attatgacta
     4261 aaaacaaaaa ataataggaa aattgataat gggaagaagt aaggttcaaa atataaatgc
     4321 aaaaattttt atttagataa gccttgattg atgggggggt tgaaaacaac aatgggtaac
     4381 ttcccagcca cccattgtac cttttaacct aaaccttaac tatgaaaaat ttatctgttt
     4441 gtttacggtt acaaagatga aacttaattt tgaattttgc aaacgattgc aaaaaaaaat
     4501 tctttttttc tcgattcttt ttttatctgt tgatgtaaat taaaagaaat caattaaata
     4561 atgaactatt taacatgtag ttaaataaag ctaaaacatt tttaaccata aaatttcctt
     4621 taatgttgta cctttgtaag cattttttta aagcttgaat catgggatta aaagaaaata
     4681 ctcatacttc cgttacggaa aatccacata aaaaacttta tatcgaaact tacggttgtc
     4741 aaatgaatgt ggctgacagt gaagtggtgg cttccatcat gcaaatggat ggctttgtac
     4801 tgacagaaga tttttcagaa gccgatgccg tattgatcaa tacctgctcc attcgcgaca
     4861 atgctgaaca acgagtgttc gaacgactta aatattttca ttcgttgaaa agaaaaaaca
     4921 aacagttgat tttgggtgtg attggctgta tggccgagcg gatgaaagaa gagttgatag
     4981 aaaaacaggg cgttgacttg gtggcagggc ctgatgctta tctcgattta cccaacttga
     5041 tgggagccgt cgaaaaaggt gagaaagcca ttaatatcca actttcgcgt acggaaacct
     5101 atcaggaagt gattcctgcc cgactggggg gccaaatctc aggttttatt tccattacgc
     5161 gtggatgcga taatttttgt tcctattgca ttgtgcctta tacgcgaggt agagagagaa
     5221 gtcgtgatgt ggaaagtatt cttaacgaat tgaaggattt gcagcagaaa aattttaaag
     5281 aagttacgct tttgggacaa aacgttaatt cctaccgtgt ggaacaggat ggaaaaataa
     5341 tcggatttcc tgaattgttg gaaattgtag ctcaggctgc acccgaaatg cgttttcgtt
     5401 ttaccacttc tcaccccaaa gacatgagcg accgcacact tgaaaccatt gctcgatacc
     5461 caaatttatg ccggtttatt catctccccg tgcaatccgg aagcaacaaa attttacggc
     5521 tgatgaaccg aaaatatacc agggaatggt atttggaacg cattgccgct atccgaagaa
     5581 ttttaccgga tgctgccatt ggaacagata ttttttgcgg gtttcatgat gaaacggagg
     5641 aagatcacaa gcaaacactt tcgttgatgc gggaagtagc cttcgatatg gcttttactt
     5701 ttaaatattc cgaacgcccg ggaacttatg ctgctcgtca tttgcccgat aacgtgagcg
     5761 aagaagtgaa attgcgtcgt ttgaatgaga ttatcgcttt gcagaatgaa ctttcgctcg
     5821 aaagcaacct gcgggatgta ggaaaaactt ttcaagtact ggtggaaggc tattccaaac
     5881 gttccaaaga gcaatttttc ggacgaactt cacaaaataa agtggtgatt tttccgcgtc
     5941 agggaaggcg aataggcgaa acggtgaaag tgaaaattaa ctcggcttcg tcggcaactt
     6001 tatttggcga agtagagcat ctgtaatttc tgaagaaaaa gactttttta atttcaggtt
     6061 atttgtgttt ggcgtttatc ggtcgaatct ataatgaagt cttttagagg atgaagtaaa
     6121 gatatttgat atcaaattct ttctaattaa attgaccaat ttttttaaaa cacctaattt
     6181 taaatagcaa accaaaaaaa gcacttaaat ctgaaaattt aaagtgcttt tttgtggtac
     6241 caccaggaat cgaaccgggg acacaaggat tttcagtcct ttgctctacc aactgagcta
     6301 tgg
//
LOCUS       DAOSCH010000109         6182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_8078_length_6182_cov_4.891301, whole genome shotgun sequence.
ACCESSION   DAOSCH010000109 DAOSCH010000000
VERSION     DAOSCH010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6182
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8078_length_6182_cov_4.891301"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /gene="purH"
                     /locus_tag="PLZ54_07685"
     CDS             complement(<1..1400)
                     /gene="purH"
                     /locus_tag="PLZ54_07685"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOJ66841.1"
                     /translation="MEKKVIKKALISVYHKDNLEEIITKLHAAGVEFISTGGTKSFIE
                     SLDIPCEAVEDLTGYPSILGGRVKTLHPKIFGGILARRDNAQDVQQMSEYEISGIDLV
                     IVDLYPFEATVAAGADEPTTIEKIDIGGISLIRSAAKNFKDVVVIASKEQYEPFLEIL
                     NSSGAQTSIEERRWFAKEAFAVSSQYDTAIFNYFDEPYQSAFRWYENHPKVLRYGENP
                     HQRGIFFGKFSEMFEQLQGKEISYNNLLDIDAAVNLINEFDDITFAVLKHNNACGIAS
                     RPSLLEAWKAALAGDPVSAFGGILITNAIIDKETALEINKIFFEVIIAPDYDLEAIEI
                     LSQKKNRIILIMKNNTLRPKQFRSLLNGVLMQDRDLHTETAADLKVVTEKSPSEAEIE
                     DLLFANKIVKHSKSNAIVLAKNKQLCASGVGQTSRVDALVHAIDKARSFDFDLKGAVM
                     ASDAFFPFADSVQLAH"
     gene            2105..2845
                     /locus_tag="PLZ54_07690"
     CDS             2105..2845
                     /locus_tag="PLZ54_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ66842.1"
                     /translation="MNLFQETGKYFLLMKRVFVQPDRSKMFFRQFPKELDKLGLQSLP
                     IVLIISVFIGAIMTIQTKLNTENPLLPTYSTGLVTRDTLLLEFSSTIMCLILAGKVGS
                     NIASEIGTMRITEQIDALEIMGVNSANYLILPKIVAFVVMMPFLVIFSMAVGLIGGYL
                     VGVFSDVITVADYLLGIQYAFNPFYVFYAMVKSLVFAFIISSVSSYYGYYAYGGALEV
                     GKASTNAVVNSSILILFFNLLITNVMLG"
     gene            2970..3737
                     /locus_tag="PLZ54_07695"
     CDS             2970..3737
                     /locus_tag="PLZ54_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ66843.1"
                     /translation="MIEVKNIIKTFDGVPALQDISAVFETGKVNMIIGQSGSGKTVLM
                     KCIIGLHQIDSGCIEYDGRNLPKMKPQEILMLRREVGMLFQGAALFDSMTVLENVMYP
                     LEMFAHESKEEIRERAYFCLRRVNLEERSYDLYPSEISGGMQKRVGIARAIALRPKYL
                     FCDEPNSGLDPLTSMIIDDLIYEITHEFNITTIINSHDMNSVMEIGEKIIYIEKGVKA
                     WEGTKDEIFDAENESFNNFVFASKLFKQLKMLKQNPY"
     gene            3778..4542
                     /locus_tag="PLZ54_07700"
     CDS             3778..4542
                     /locus_tag="PLZ54_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOJ66844.1"
                     /translation="MKIISYNVNGLRAALTKDFIQWLQAENPDVLCLQETKAQPDQID
                     TLLFAELGYTAYLHSAQKKGYSGVATFTKIPPDYVYFGMDNPEFDTEGRVLRTDFGDI
                     TVINVYVPSGSSGGERQDFKMKFLEEFLKFIQNLLRERPNLVVCGDYNICHKPIDINH
                     PERQIGVSGFLPEEREWMDRFQALGMVDSFRVFDQSPEKYSWWSYRGGARYRNAGWRI
                     DYHWVSEPLRSRLKAAAILSEAFHSDHCPVMVELDV"
     gene            4660..6027
                     /locus_tag="PLZ54_07705"
     CDS             4660..6027
                     /locus_tag="PLZ54_07705"
                     /inference="COORDINATES: protein motif:HMM:NF039698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6242 domain-containing protein"
                     /protein_id="HOJ66845.1"
                     /translation="MLRNQLKILALLGTIFFLNSCLGDGNVKEISSDPSFVSLTFSAN
                     NKIPNLKDAVFSLQYDSVIVNLDSLPYQTRIDSVYATFTFKSTYAAYLVYENDSTALI
                     SGNEAFDFTKIKAIINIASDGVTRRKYLIKVNVHQVEPELYIWQKVNDAAHSLNVQHQ
                     KAVYFNDRLYLFVNDRQQNYLFVSPNGVSWTQDVISGLPTNATFEDITVFNNQLYVTC
                     GNSNIFSSGDGLTWTRQTYSEYEFTSLIFVLNDRLWAVSHSLTDGKLRFASSVDGTEW
                     LIYNEIPENFPVRGFASVVFNTKVNKQKGLVLTGFSSDGTALKTNWNTEDGIYWVDFS
                     AENASLDSIGRGASVINYDDKLLLFGQDNKPIPESYFRQSVDQGFSWQVPDSNLNNLP
                     KEMGIRHYSSVVVYRPRTYSSTDSKQEIESSNWIFIIGGKNAYDEYLTDVWKGKVNRK
                     NFLRQ"
BASE COUNT         1868 a         1158 c         1178 g         1978 t
ORIGIN      
        1 tcgtgagcca gttgcactga atcggcgaac gggaaaaagg catcggaagc catgacggca
       61 ccttttaagt cgaaatcgaa agaacgcgcc ttgtcaatgg catgaaccaa tgcatccact
      121 ctcgaagttt gtccgacacc actggcacac aattgtttgt ttttcgccag tacaatggca
      181 tttgattttg aatgttttac aattttattt gcaaaaagta aatcttcaat ttcagcttct
      241 gaaggcgatt tttcggtaac tacctttaag tcagcagccg tttcggtatg caagtctcta
      301 tcctgcatca gcactccatt cagtaaggaa cggaattgct tgggacgcag tgtattgttt
      361 ttcattatca aaataatccg atttttcttt tgacttaata tttcaatggc ttccaaatca
      421 taatcgggag caatgattac ttcaaaaaat attttgttga tttccagagc tgtttctttg
      481 tcaatgatgg cattggtgat gagaattccg ccgaaagcag aaaccggatc gccagccagc
      541 gcagctttcc atgcttcaag aagtgaagga cgtgatgcaa ttccgcaggc attgttgtgc
      601 ttgagaacag caaaggttat gtcgtcaaat tcattgataa gatttacagc ggcgtcaatg
      661 tcgagcagat tattgtaaga aatttccttt ccctgaagtt gttcaaacat ttcgctgaat
      721 ttgccaaaga aaattccgcg ttgatgcgga ttttcgccat aacgtaaaac ttttggatga
      781 ttttcatacc agcgaaaagc actttgataa ggttcatcga aatagttgaa aatagctgta
      841 tcgtactgag aggaaacagc aaaagcttct ttggcaaacc atcggcgttc ctcgatggaa
      901 gtttgagctc cactgctgtt taaaatttcc agaaaaggtt cgtattgctc tttggaggcg
      961 atgacaacca catccttgaa attttttgct gcagaacgga taagcgaaat tccaccaata
     1021 tcaatttttt caatggttgt tggttcatct gcacctgccg caacagtggc ttcgaaggga
     1081 tacaaatcta caatgaccaa atcgattccc gaaatttcat attcagacat ttgctgcaca
     1141 tcctgagcat tgtcccgccg cgcgagaata cctccaaaga ttttcggatg cagcgttttt
     1201 actcttcctc caagaattga agggtagccg gtcaaatctt ctactgcttc gcaaggaatg
     1261 tccagcgatt caataaacga ttttgtgcct ccggtagaaa taaattctac tccagcagca
     1321 tgcagtttgg tgattatttc ttccagattg tctttgtgat agaccgatat taatgctttt
     1381 ttgattacct ttttttccat gcctttgtta ctttggttat gggatgcaaa gttacacgaa
     1441 aattttcaat ttacacacag acaaggtaaa atcgaaattt ttccttgtaa atttgttcat
     1501 cacaccataa cttttacaga aaaaactatt aaaaaatctt attgtgtttt aaaagttgct
     1561 tttgcaaatc ttcaaacaaa aagacaaagt aaaattagtc cctaatgatc gaacttttta
     1621 atttcattcc aaaatttatc cccaatcaga aattttttgg aaacaaaccc ttcttacacg
     1681 atttgcttct attttattga tacataaata atcctgaccc aactcaaact tcttcttatc
     1741 aaaataattt ttgaatatat ctacttttgc accttcggtt aatattgcat gaataaccga
     1801 aagacacgag acaggctatc aagtaaataa atccatgctt tatcgtaaag attaagattc
     1861 tgtttatttt tttactgaag cctgtgaaat tttattattc tgtttatttt gattttatcg
     1921 ctaaaataag aaattctaac acattctttt ttgcttttga atagtagtgt actaattcat
     1981 caattgcaaa aattaagtat atttgcaact tgtttgcgaa acaaaataag aactttttgc
     2041 ttcaaaactt gttggatggt ttgtcgacaa ttttttaact gcttgaaatt ttgaaaattt
     2101 gcgtatgaat ttatttcaag aaacgggaaa atattttttg ctgatgaaac gtgtgtttgt
     2161 acagcctgat cgttcgaaaa tgtttttccg ccaatttccc aaagaattgg ataaactggg
     2221 tttacaatct ttgcccatag tattaatcat ttctgtattc atcggggcta tcatgaccat
     2281 tcaaaccaaa ctcaatacgg aaaatccctt acttccaacc tatagcactg gattggtaac
     2341 ccgcgataca ttgctgctcg aattttcttc taccatcatg tgtctgattc tggccggcaa
     2401 agtcggttcc aatatcgctt ccgaaattgg taccatgcgt attaccgaac aaattgatgc
     2461 actcgaaatt atgggcgtca attctgccaa ttatcttatt cttccaaaaa ttgtggcttt
     2521 cgtcgttatg atgccgtttt tggtaatttt cagcatggct gtgggactaa ttggcggtta
     2581 tttggtcggc gttttcagcg atgtcataac ggtggccgat tatctgctgg gtatccaata
     2641 tgcctttaac cctttttatg tgttttatgc aatggttaaa tcgctggttt ttgctttcat
     2701 catttcttcc gtttcttcct attatggcta ttatgcttac ggaggagctt tggaagtggg
     2761 taaagcaagt accaacgccg tagttaacag cagtatctta attttgtttt ttaatctatt
     2821 gattaccaat gtaatgttgg gataaaaaat actttctgcg ctttcaatct tcagcagaaa
     2881 ctttaaaatg aaaagatttt tttctaaaaa caatgtttgt ataagggaga aataactttt
     2941 aaaaataaca gaccaacttc aaaccaagca tgattgaagt aaaaaacata ataaaaactt
     3001 ttgatggcgt tccggcattg caggacattt ctgccgtttt tgaaaccggc aaggtaaata
     3061 tgatcatcgg acaaagtggt tcgggcaaaa cggttttgat gaaatgtatt atcggccttc
     3121 accaaattga tagcggctgc atagagtacg atggacgaaa tttaccaaaa atgaagcctc
     3181 aagaaatact tatgctgcga cgtgaagtgg gcatgctttt tcagggagct gccctattcg
     3241 attccatgac ggtgctggaa aatgtaatgt atccactgga aatgtttgcc catgaaagta
     3301 aagaggaaat aagggaaaga gcttattttt gccttcgtcg cgttaatctc gaagaaagat
     3361 cttacgatct ttatccttcc gaaatcagtg gcgggatgca aaaacgggtg ggtattgcgc
     3421 gtgccattgc tttacgacct aaatatcttt tttgtgatga acccaattcc ggactcgatc
     3481 cccttacctc tatgattatt gacgatttga tttatgaaat tacacacgaa ttcaatatta
     3541 ccaccattat caactcacac gacatgaatt cggtaatgga aattggcgaa aaaatcattt
     3601 atatcgaaaa aggagtgaaa gcttgggaag gcacaaaaga tgaaattttt gatgctgaga
     3661 atgaaagctt caataacttt gttttcgcat ccaagttatt taaacagtta aaaatgttaa
     3721 aacaaaatcc ttattaattc atttgctgaa aatttgaacc tcaagcaagc aatatccatg
     3781 aaaattatct cctacaatgt aaacggttta agagctgcac tgaccaaaga ttttattcaa
     3841 tggctgcaag cggagaatcc tgatgtcctt tgtctgcagg aaacaaaagc acagcccgac
     3901 caaatcgaca ccctactttt tgccgaactg ggttataccg cttatcttca ttcggcacaa
     3961 aaaaaagggt acagtggcgt tgctactttt actaaaattc cgcccgatta tgtttatttc
     4021 ggaatggata atcccgaatt tgacactgaa ggaagagtat taaggacaga ctttggcgat
     4081 ataacggtta tcaatgtata tgttccttcc ggatcatcag gcggagaaag acaggatttc
     4141 aaaatgaaat ttctagaaga atttctgaaa tttatacaaa acttgcttcg tgaaagaccc
     4201 aatttggtgg tttgtggcga ttacaacatc tgccacaagc ccattgacat taatcatccc
     4261 gaacgtcaaa taggagtatc agggtttcta cccgaagagc gggaatggat ggatcgattt
     4321 caggctttgg gaatggtaga ttcgtttcgg gttttcgacc agagtccgga aaaatatagc
     4381 tggtggagtt atcgtggtgg tgcgcgttac agaaatgccg gctggcgcat cgattatcat
     4441 tgggtaagcg aaccgctgag gtctcgactg aaagcggctg ctatccttag cgaagctttt
     4501 cattccgatc attgtcctgt catggtggaa ctcgatgttt aattaatttt aagtaatact
     4561 tatccgctaa agtaataaaa aacaataaaa tatcgacctt ttcggcgcaa caactgaaat
     4621 gttttttaac tttgctcctt cattcaaaat ttattgttca tgctgcgaaa tcaattgaaa
     4681 atacttgctt tgttaggtac tatttttttt ctaaattcct gcttggggga tggtaatgta
     4741 aaggaaattt cttctgatcc aagttttgtt tcgcttactt tttcggccaa caataagata
     4801 cccaatctaa aagatgctgt ttttagtctc caatacgatt cggtgattgt caaccttgat
     4861 tcgctaccct accagacaag aattgacagt gtttatgcca ctttcacctt taaaagcacg
     4921 tatgctgcct atttggttta tgaaaatgat tcaacagccc ttatttccgg aaatgaagct
     4981 ttcgacttta caaagattaa ggcaattatt aatattgctt ctgatggagt aacaaggcgc
     5041 aaatatctga tcaaggtaaa tgttcatcaa gtggaaccgg aattgtatat ttggcaaaag
     5101 gtaaacgatg cggctcactc tttaaatgtt caacatcaaa aggctgttta tttcaacgat
     5161 cggttgtatc tttttgtaaa cgaccgccag cagaattact tgtttgtttc tcccaatggg
     5221 gtatcatgga ctcaagatgt tatttcggga cttcctacaa atgcaacttt tgaggatatt
     5281 acagttttca acaatcagct ttatgtaaca tgcgggaatt cgaacatttt ttcttcaggt
     5341 gatggattga catggacaag acagacttat tccgaatacg aatttacttc actgattttt
     5401 gttttaaacg atcggctttg ggctgtttcc cattctttga cagatggaaa acttcggttt
     5461 gcttcttctg tcgatggtac agaatggctg atttacaatg aaattcctga aaattttccg
     5521 gttcgtggat ttgcctcagt ggtattcaat acgaaagtga acaagcagaa aggactggtg
     5581 ttgaccggtt tttcttccga tggaactgct ttgaaaacaa attggaacac agaagatggc
     5641 atttactggg ttgacttcag cgccgagaat gcttcgcttg attctatagg gaggggagct
     5701 tcggttataa attacgatga taaactctta cttttcggac aggataacaa accaattcca
     5761 gaaagttatt tccgtcaatc tgtcgatcag ggtttcagtt ggcaagtccc ggacagtaat
     5821 ctcaataatc ttcccaaaga aatgggaata cgtcattatt cttcagtcgt agtttatcgg
     5881 ccacgtactt acagctctac tgactcaaag caggaaatcg aatcttctaa ctggatattt
     5941 attattggcg gaaagaatgc ttatgatgaa tatctcaccg acgtttggaa aggtaaagta
     6001 aaccgaaaaa actttttacg acaataaaag tttaactata agctaattac ttactaagcg
     6061 ccttcataaa aggcgctttt tttattaatt ggcagcccat aatatagaaa aaagttaaac
     6121 tcacccccaa atctttaacc gagaaaaaag ttttaaaaga aaatgagtag caataaaaca
     6181 ca
//
LOCUS       DAOSCH010000110         6032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_8321_length_6032_cov_5.779488, whole genome shotgun sequence.
ACCESSION   DAOSCH010000110 DAOSCH010000000
VERSION     DAOSCH010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8321_length_6032_cov_5.779488"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="PLZ54_07710"
     CDS             complement(<1..949)
                     /locus_tag="PLZ54_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66846.1"
                     /translation="MKKLTILLICVWAMMLAFTFSSSAQTVFALQNGSRTQFYTTLSS
                     AISNIQDGDTLYLPGKPLDGDYTFAKKVTIIGAGYDPDSAAATGITKITGRVYFAKNS
                     KGSSMTGIRTEGRVYVRDSLITITRSNLESLEIQNGSNPIGFVYVGDCIIRNNIEGAN
                     DVVTNVLIERCILNGNLGGGNKTNNLVIKNCVLTYNGNYFLYSISNIIYQNSIILNTN
                     NYMFTAGSSSLTFTNNLFVRPAFSNNTYVMTGNIFGVPESDIFVDSANGNYHIKPTCT
                     QALTLATDGKEVGIYGTDNPFLVPTFAPRFISINNAESTV"
     gene            complement(1333..1406)
                     /locus_tag="PLZ54_07715"
     tRNA            complement(1333..1406)
                     /locus_tag="PLZ54_07715"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1370..1372),aa:Ala,seq:ggc)
     gene            1493..1807
                     /locus_tag="PLZ54_07720"
     CDS             1493..1807
                     /locus_tag="PLZ54_07720"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HOJ66847.1"
                     /translation="MLIFNTTYLVENEFYPQWINWIQESLIPFMMSTAGFSEPQIAKV
                     LSGESVKETSYSVQFKISDKSALNDWMRQYGEDFAKLCSANFGNHVLFFSTMLELISD
                     DK"
     gene            1797..2381
                     /gene="ruvC"
                     /locus_tag="PLZ54_07725"
     CDS             1797..2381
                     /gene="ruvC"
                     /locus_tag="PLZ54_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOJ66848.1"
                     /translation="MINDQIILGIDPGTTIMGYGVLNIRAHQPELMAMGILDLKKYAN
                     HYVKLQRIFARTLSLIDEFHPACMAIEAPFFGKNVQSMLKLGRAQGVAMSAALYRDIP
                     ITEYAPLKIKMAITGNGNASKEQVADMLRRFLHIPQENMFPQLDATDGLAAAYCHFLQ
                     LGKPESQKVYSGWKDYISKNQEKVKNPIQKKREK"
     gene            2472..3572
                     /locus_tag="PLZ54_07730"
     CDS             2472..3572
                     /locus_tag="PLZ54_07730"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HOJ66849.1"
                     /translation="MKKSFLLVLCLLFLFQSGEGKIYRFLVGTYTQNTVSEGIYSLSF
                     DAESGVCKIQLAATNVDNPSFLTFSPDKNFVYSVNESGTENKIASFRFDATSGKLFFI
                     NRVEANGSDPCFITASKQHVITANYSSGNACVFKRNSDGSIGETVQKIQFNGHSIDSM
                     RQTSSHVHQTIFSPNGHFLLINDLGTDLIHSFRYVPNRSENCLLPYDSLKVKQGRGPR
                     HLTFSKDGKFVYLLHELDGTICIIGFNKGKLLFYGENSVATNKKLKTGAADIHISPDG
                     KFLYATNRGEANNITCFKILKNGLLEFVEQNSTQGKGPRNFAITKDGHFLLVGNQYSD
                     EIVVFKRDRENGKLMDTKIRIKIGAPVCLLEY"
     gene            complement(3582..5855)
                     /locus_tag="PLZ54_07735"
     CDS             complement(3582..5855)
                     /locus_tag="PLZ54_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOJ66850.1"
                     /translation="MADEKSIQEQEREEERMIQQEFEGLLEDYRNSNHRQKVEIITKA
                     FNFANTAHKGMKRRSGEPYIMHPLAVARIVVKEIGLGSTSICAALLHDVVEDTEYTVE
                     DIRHHFGDKIAQIVEGLTKISGGVFAEKASEQAENFRKLLLTMSDDIRVILIKIADRL
                     HNMRTLGSLPPAKQYKISGETQYIYAPLAHRLGLFRIKTELENLSFKYEHPDIYKSIE
                     NKLLMDEEARNAFYKEFSAPIREKLTEMGYEFTLKERVKSVYSIWHKMSTRNIPFEEV
                     YDILALRIIFKPKEGMPEKDQCWMIYSAITALYKPHPERIRDWLSTPKANGYEALHVT
                     VMGPNGRWVEVQIRTERMNEIAEKGLAAHWKYKLGENNESELDNWLKTIKEVLEHPDT
                     DAINFLETFKLTLFASEIFVFTPKGEIKTIAQGATALDFAFMIHSDLGMHCIGAKVNH
                     KLVPLNHRLQSGDQVEILTSQKQTPQEEWLNYVTTAKARSKILELLKKEEKNIAAEGQ
                     KMIENALAEINLPASNENIMKILQHYNLTQRNELYVQAGKGALNLDDIKKVIFKQKTQ
                     NVFSKYLKIPFIGSSSSDNKEIIIPTQGIDRKATLKLTEENTGKIFKLAECCHPIPGD
                     DVLGYLDENGIIYIHKRQCPVALKLKSNFGERILSAEWATHKILSFEETIEIKGIDRK
                     GILIEILKVISEAYGVNISKINIETESGIFIGKFQVLVHDTEELNNMCENLTKIPEVQ
                     SVQRIEQTNLQPQEQKT"
BASE COUNT         1765 a         1067 c         1197 g         2003 t
ORIGIN      
        1 tgacggtact ttcggcattg ttaatggaaa taaatcgtgg cgcaaacgtt ggaaccagga
       61 atggattgtc cgtgccataa attcccactt cctttccatc ggtagccagt gtcagtgcct
      121 gtgtgcaggt cggtttgata tgatagttcc catttgctga atctacaaat atatcacttt
      181 caggcacccc aaatatattt cccgtcataa cataagtatt attagaaaag gcaggacgta
      241 caaataaatt gttagtaaac gtaagagaag aactaccagc tgtaaacata tagttattgg
      301 tgtttaaaat tatcgaattt tgatatataa tgttgctgat actatataaa aaataattac
      361 cattatacgt taaaacacaa ttttttatca ctaaattatt ggttttgttt ccacctccca
      421 aatttccgtt gagaatgcag cgttctatca atacattggt aaccacatca tttgctcctt
      481 caatgttatt tcgaataata caatcgccca catatacaaa tccaatcgga ttactgccgt
      541 tttgaatttc aagcgattcc agattacttc gcgtaatggt aatcaaggaa tcacgcacgt
      601 aaacacgccc ttccgtgcga ataccggtca tggaagatcc tttgctgttt ttggcaaaat
      661 agactctgcc ggtaattttt gttattcccg tggccgcagc actgtcgggg tcatagccgg
      721 caccgataat ggtaaccttt tttgcaaaag tataatctcc atctaacggt ttccccggca
      781 aatagagtgt atctccatcc tgaatgttac tgatggcact ggacaaagta gtataaaact
      841 gggttctgct tccattttga agcgcaaaaa cggtttgggc actggaagaa aatgtgaaag
      901 cgagcatcat agcccataca caaataagga gaatagttaa ctttttcata agctgagttt
      961 taatagtaat aataatggtt taatttaatt ttacaatttt ttttgttcaa taataaagat
     1021 aatatataat gcagggaatc gtgttttttt aaaaataagg agaataaaaa ctaaagtata
     1081 tctattgata ttcaattgtt tactttgtaa tattttaata atagatattg aaatcaagca
     1141 attattcagt tttccaccat tgattttttt aaggaattcc tttttatgca aatgtattta
     1201 gtaattgacg acattggtct gcaaaaggta aaaaaaaata agcaaaaggt aaaaaagtga
     1261 taaatatttc ttttttcaat ggatggaaag gaggaaaaat aaaaaaagct taccaaagat
     1321 aagcttttcg agtggagatt accggagtcg aaccggttac ctcgacactg ccagtgtcgc
     1381 gctctagcca aatgagctaa acccccaaaa tgtatatttt ttcactgcaa agttaaagaa
     1441 ctttttcaaa ttatgcttac ttttgttcaa attattccat aaaaaattac aaatgctgat
     1501 ttttaataca acttatttgg ttgaaaacga attttatcct cagtggataa attggattca
     1561 agaatcattg attccattta tgatgagcac tgccggtttc tcggagcctc aaattgccaa
     1621 agtgctttcc ggtgaaagcg tgaaggagac ttcctattcg gttcagttta aaatttccga
     1681 taaaagtgct ttaaacgatt ggatgaggca atacggagaa gattttgcaa aattatgttc
     1741 agctaacttt ggaaaccatg ttcttttctt ttcaaccatg ctcgaattga ttagtgatga
     1801 taaatgacca gattatattg ggtattgatc cgggaactac cattatggga tatggagtgc
     1861 tgaatatcag ggctcatcaa cccgaattaa tggctatggg aattcttgat ttaaaaaagt
     1921 atgccaatca ttatgtaaaa ttacaacgta tttttgctcg aacattgtcg cttatagatg
     1981 aatttcatcc ggcctgcatg gccatagaag ctcctttttt cgggaaaaat gtgcagtcga
     2041 tgctgaaact tggaagggcg cagggcgtag ccatgtcggc agctctgtat cgtgatattc
     2101 ccattacgga atatgcaccg ttgaaaataa agatggctat aacgggcaac ggaaatgctt
     2161 caaaagaaca ggttgccgat atgttgcgca ggtttttgca tattcctcag gaaaacatgt
     2221 ttccacagct ggatgctact gatggcttag cagcagccta ctgtcatttt ttacagttgg
     2281 gaaaacccga atctcagaaa gtttattccg gctggaaaga ttatatttca aaaaatcagg
     2341 aaaaagtgaa aaatcccata cagaaaaaaa gagaaaaata gatttttcag tcaagaaaga
     2401 aacgtttaga gctttcaaac agatttatat aacatgttga ttaacaatat actgatttat
     2461 aaatattcat aatgaaaaag tcatttttat tggttttgtg tttgttgttc ctttttcagt
     2521 cgggagaagg aaaaatatat cgttttttgg ttggaactta tacgcagaat actgtgagcg
     2581 aaggaattta ttctttaagt tttgatgctg aaagtggagt ttgtaagatt caacttgctg
     2641 caactaatgt ggataatcca agttttttga cattttcgcc cgacaagaat tttgtttatt
     2701 ccgtcaatga gtcgggtact gagaataaaa tcgcatcttt tcgttttgat gccacttcag
     2761 gcaaactgtt ttttattaac cgtgtggaag caaatggctc ggatccctgt tttattactg
     2821 cttcaaaaca acatgttatc acagccaatt attcaagtgg caatgcttgc gtttttaaga
     2881 gaaactcaga tggttcgata ggtgaaacgg ttcaaaaaat tcagttcaat ggacacagca
     2941 ttgattccat gcgtcagaca agttctcatg tacatcaaac gatattttca ccaaacggac
     3001 attttttatt gatcaacgat ttgggaaccg atttgataca ttcttttcgt tatgttccca
     3061 atagatcaga gaattgtctt ctaccttatg acagtttaaa agttaagcag ggtagaggac
     3121 ctcgacattt gactttctcc aaagacggaa aatttgtcta tttgcttcac gaattggatg
     3181 gaacgatttg cattatcgga tttaataaag gaaaattgtt gttttatggc gaaaattctg
     3241 tagcaacgaa taaaaaattg aaaaccggag cggccgacat tcatatttct cccgatggaa
     3301 agtttctgta tgccaccaat cgtggagaag ccaacaatat tacctgtttt aaaatactga
     3361 aaaacggttt gttggaattt gttgagcaaa attcaactca gggcaaaggg cctcgtaatt
     3421 ttgcaattac caaagacggt cattttttgt tggttggcaa tcagtattcc gacgaaattg
     3481 tggtttttaa aagggacagg gaaaacggga aactgatgga tacgaaaata cggataaaaa
     3541 ttggagcgcc tgtttgcctg ctcgaatatt gagaggtttt attaggtttt ttgttcctga
     3601 ggttgcaggt tggtttgttc gattcgttga acagactgaa cttcgggaat tttggtcaga
     3661 ttttcgcaca tgttgttgag ttcttcggtg tcgtgtacca acacttgaaa tttgccgata
     3721 aaaattccac tttccgtttc gatatttatt ttgctgatgt tgacgccgta agcttcagaa
     3781 attactttta aaatttcgat cagaattcct ttgcggtcaa ttccttttat ttcgatagtt
     3841 tcttcgaaag atagaatttt atgtgtggcc cattcggcag aaagaattct ttcgccaaag
     3901 ttacttttta atttcaatgc aaccggacat tgtcgtttgt gaatatagat aatgccgttt
     3961 tcgtcgagat aacccaacac atcatcaccc ggaatgggat gacagcattc tgccagtttg
     4021 aatatttttc ccgtgttttc ttcggtcagt ttcagggtag cctttctgtc tattccctga
     4081 gtggggataa taatttcctt gttgtcggaa gaagagcttc cgataaaggg aatttttaaa
     4141 tatttggaga aaacattttg cgttttctgt ttaaagataa cttttttaat atcgtcaagg
     4201 tttagagctc ctttacctgc ctgaacataa agttcatttc gttgggtaag gttatagtgc
     4261 tgaagaattt tcatgatatt ttcgttgctg gcgggcaggt ttatttcggc caacgcattt
     4321 tctatcattt tttgaccttc cgccgcaatg tttttttctt cttttttcag gagttcgaga
     4381 attttgcttc tcgctttggc tgtagtaacg taattcaacc attcttcctg aggagtttgt
     4441 ttctgcgaag ttaggatttc aacctgatcg ccgctctgca aacggtggtt caagggcaca
     4501 agtttgtgat tcactttggc tccaatgcag tgcataccca aatcagaatg aatcataaag
     4561 gcaaagtcga gagcggtggc tccctgagca atggttttga tttctccttt tggtgtaaaa
     4621 acaaaaattt cggatgcaaa taaggttaat ttgaaagttt cgaggaaatt tatggcatcc
     4681 gtatccgggt gttcaagtac ttctttaatg gttttcagcc aattgtccaa ttcagattca
     4741 ttgttttctc ccagtttgta tttccagtgg gcagccaacc ctttttcggc aatttcattc
     4801 attctttccg tacgaatttg tacttccacc caacgaccgt ttggccccat taccgttacg
     4861 tgaagtgctt catatccgtt ggctttgggt gtactcagcc agtctctgat gcgctcggga
     4921 tggggtttgt aaagtgcagt aatggcggaa taaatcatcc agcactgatc tttttcaggc
     4981 ataccttctt tgggcttgaa aataatgcga agtgccagaa tatcatatac ttcttcgaaa
     5041 gggatattgc gggttgacat tttgtgccaa atggaataga ctgattttac acgttctttc
     5101 agggtaaatt catatcccat ttcagtcaat ttttccctga taggagcaga aaattcctta
     5161 tagaaagcat ttctggcttc ttcgtccatc aatagcttgt tttcgatgga tttgtaaata
     5221 tctggatgtt cgtatttgaa actgagattt tcgagttcgg ttttaatgcg aaaaaggcca
     5281 agtctgtggg cgagtggagc ataaatgtat tgggtttcgc ccgaaatttt gtattgtttg
     5341 gccggaggaa gtgatcccaa agtgcgcata ttgtgcagtc tgtcggcaat tttaatcagg
     5401 atgacgcgaa tatcgtccga catggtgaga agaagttttc tgaaattttc ggcttgttca
     5461 gaagcttttt cggcaaaaac gcccccggaa attttggtaa gtccttctac aatttgtgca
     5521 attttgtcac caaaatgatg gcgaatatct tcaacagtat attcggtatc ttctaccaca
     5581 tcgtgcaaca aggcggcaca aatggaagta gagcccaagc ctatttcttt tactacaatt
     5641 cgtgccacag ccagtggatg catgatgtat ggctcgccgg aacgtcgttt catgccttta
     5701 tgagcagtat tggcaaaatt aaaggctttt gtaatgattt cgactttctg ccggtgattg
     5761 gaatttcgat agtcttcgag caaaccctca aattcctgtt gaatcattct ttcttcttct
     5821 ctttcctgtt cttgaatact cttttcgtcg gccataaact tgcatcctat tttatgcaaa
     5881 tataaataat ttattcaatt tcgatttgtt tttttacaac aaaaactcat ttcaatccat
     5941 tattgaaaac aattttcaga ttgaaagagt tcgacaaagt caatcagaat gaattttatt
     6001 ctaataacct gctaacggtt tggcgcttgg cg
//
LOCUS       DAOSCH010000111         6016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_8351_length_6016_cov_5.531622, whole genome shotgun sequence.
ACCESSION   DAOSCH010000111 DAOSCH010000000
VERSION     DAOSCH010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8351_length_6016_cov_5.531622"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PLZ54_07740"
     CDS             <1..718
                     /locus_tag="PLZ54_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66851.1"
                     /translation="LSATTHNFVGNNPSPKEFFVLEYRKKTGWDAYLPAEGMLIWHID
                     YDQTAWDNNEPNNYTGTAQTASSHMRVYLQPLSGSTTTPGTAFTSGSFTPTTWSGTNI
                     NRAITEISKSSDQVTFKLMGGEIEDPNKAVIKLGVIQSQLVFPYTAVNNAALKVINIK
                     TNGITGNLTVTLSGDQAEMFSVSANSITQNAANSTDGVNLNITYRPTTSGEHTAVLTI
                     SGGGLSPDKVILLKGTGQPQ"
     gene            1196..1873
                     /locus_tag="PLZ54_07745"
     CDS             1196..1873
                     /locus_tag="PLZ54_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ66852.1"
                     /translation="MSKKKGKGQDELANVEHALSASELFFEKYQKQILYVVGALIMVV
                     LVVLAVRNFYLTPREKAAQNEMYHAQYYFNLDSFQVALNGKEPEFMGFKQIVSEYGIT
                     PSGNLAAAYAGICYYKLGQYENAIKYLTQFDGNNSYFSTSVIGLLGDCFVEIGQNDKA
                     LTYFNKAIAVENEVLSPLYLKKAGLLCESMNQLKKAEEYFLEIKEKYPKSMEAGDIDK
                     YLARVQN"
     gene            1952..2473
                     /gene="ribH"
                     /locus_tag="PLZ54_07750"
     CDS             1952..2473
                     /gene="ribH"
                     /locus_tag="PLZ54_07750"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOJ66853.1"
                     /translation="MSTEYKNLSSYNEDEMPDKSKVFLQQYGIVVADWNPQITHAMLE
                     GAYNALIENGSNPELINIVHVPGTFELTYGAKQLLEQYEEMDHIKRHKYAAIIALGCV
                     IQGETPHFNYVCEGVTNGIAYLNANAVGCPVIFGVLTTKTLQQALDRAGGINGNKGVE
                     AAITAIKMANIDW"
     gene            complement(2647..3465)
                     /locus_tag="PLZ54_07755"
     CDS             complement(2647..3465)
                     /locus_tag="PLZ54_07755"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOJ66854.1"
                     /translation="MLLALFLLPACDNRPKNVLSKKEMVKMLTEYHLLHGTLQTTNQY
                     YTPEGTLYFESLLKKHGITKAEFDSSMVWYAKNPKKFERIYAEVLQNLNHLREDIVKG
                     KFRSPDSILPGIEISNLWNLHPSYRFTPDSARTKLPFKIQQPYLMTQDVYELQFLERI
                     APEDSCVDPYVVMKIHYANGKTDSIFQKTFNDSILRRYTLRLRARFPYKVDSVTGLLL
                     GCKQYNGNFNVQLDSIRLLRYFVPQWQDSLKQVVEKNAAPPQKIQTKTIRPKEN"
     gene            complement(3482..4090)
                     /locus_tag="PLZ54_07760"
     CDS             complement(3482..4090)
                     /locus_tag="PLZ54_07760"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOJ66855.1"
                     /translation="MSTTKKSVLLIIFVLLVDQASKFYIKTHFALGDHIDIFSWFQIY
                     FIENNGMAFGIELLDKLFLTAFRIGAVVLIIFYIRSLIKRNFRTGYVLSISLLLAGAA
                     GNIFDSVFYGVLFSDSYGRVATFLPPEGGYAPLFYGKVVDMLYFPLIKNAAGETIFFR
                     PVFNVADAAITISVFIILIFYRKELNASFEKKEKTNVAEQSK"
     gene            complement(4174..4551)
                     /locus_tag="PLZ54_07765"
     CDS             complement(4174..4551)
                     /locus_tag="PLZ54_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOJ66856.1"
                     /translation="MSEKTRYSDEELEEFRQIILEKLEKAQKDYELLRNGVTNSDGND
                     VTDTSPTFKVLEEGASTLSKEEAGRLAQRQLQFIQHLQAALVRIENKTYGICRETGKL
                     IPKERLRAVPHATLSIEAKKSKK"
     gene            complement(4687..>6016)
                     /locus_tag="PLZ54_07770"
     CDS             complement(4687..>6016)
                     /locus_tag="PLZ54_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HOJ66857.1"
                     /translation="TIEKYGSDPLRWYMMTNSAPWDNLKFDIEGVEEVRRKFFGTLYN
                     TYSFFALYANVDEFTCSEPEIPLENRPEIDRWIISLLNTLIKEVGEYYDDYDPTRAGR
                     AISDFVTENLSNWYVRLNRKRFWGGEYNKDKISAYQTLYTCLETVAKLMAPIAPFYAD
                     QLFLDLNEVTEKNNCESVHLSNFPVYDEKLIDKNLEECMQLAQQISSMILSLRRKAGK
                     KVRQPLSKAIVPAADDKIYQQISYVADLIKAEVNIKELQVIPANMEMANLTKKVKLNF
                     KVLGKKYGKQMKEIAEATAKLSNKEIAELEQNGIYILNLPGGEVQLTTDDVEIITEDM
                     PGWLTANEGKLTVALDITVTDELLKEGIARELVNRIQNIRKQNGYEITDRITVEIEKR
                     DEINDAVTEFSNYISSQTLAAGISLVDKLEDGIELDFDEYMVKVKVEKTR"
BASE COUNT         1863 a         1165 c         1112 g         1876 t
ORIGIN      
        1 actttcggct actacacaca actttgtagg aaataatccc tctccaaaag agttttttgt
       61 actcgaatat cgtaaaaaga ccggttggga tgcttatcta cctgccgaag gaatgcttat
      121 ttggcacata gattacgatc aaacggcttg ggacaacaac gaacccaaca actacaccgg
      181 aactgctcaa acagcaagca gccacatgag agtttacctg caacctttga gcggctctac
      241 caccactccg ggaacagctt tcacaagcgg atcattcaca ccaacaacat ggtcgggaac
      301 aaacattaat cgagctatca cagaaatttc caaatcatct gatcaagtta ctttcaagtt
      361 gatgggtggc gaaatcgaag acccaaacaa agctgtgata aaactcggcg ttattcagtc
      421 tcaacttgtt tttccttaca cagccgtaaa caatgctgcc ctgaaagtaa tcaacattaa
      481 aacgaacgga ataaccggga atttgaccgt taccctcagc ggcgatcagg cagaaatgtt
      541 ttccgtttcg gcaaattcca ttactcaaaa tgcagccaat agcactgatg gtgtaaatct
      601 caacattact tatcggccaa cgacttcagg cgaacacacc gctgtgctga ctatttcagg
      661 tggcggactt tctcccgaca aagttattct gctgaaagga accggacaac cacaataaaa
      721 tggttgaatg aacattgacg aaaatcgatt taaaaagtaa gcgcctactt tttaaatcga
      781 tttttttcat gtttaaaaat attgcccact atagggtaac atgggtgtct tgaaaaagta
      841 cggaaaacag gagaaaaaag aaaaacctta tccataacct tgtaatctta gtaaccattt
      901 aatgtttctt ggctggcacc tttatatcgt tgttgtttgg tttttcaaac tgtattgtat
      961 atcggctgat tcaaatttgc aaaaaagtca tggttttttt tatgatgcat gaataaactg
     1021 aaatttacca aaattccaat ttatcaaaca ttttatgtaa atttcgcact ggctggttga
     1081 gtctgctatg tgaaaaactt accataaaat ttcattatcc gaaagaaaat ctttaatttt
     1141 gcattctatt atttaaacaa cacacaaatt taaaatccta aagataacca ttcttatgtc
     1201 gaagaaaaag ggaaaaggtc aagacgagtt ggcaaatgtg gaacatgctt tatcagcatc
     1261 cgaactgttt ttcgaaaagt atcagaagca gattctatac gtagtaggag ctttgataat
     1321 ggttgttctg gttgtattgg cagtacgtaa tttttatttg actcctcgtg aaaaagcagc
     1381 acaaaatgaa atgtaccatg ctcaatacta tttcaatctc gattcgtttc aggttgcact
     1441 taatgggaaa gaacccgagt ttatgggttt taaacaaata gtatcggaat acggaataac
     1501 accttcgggt aatttggccg ctgcatatgc cggtatttgc tattataagc tagggcaata
     1561 tgaaaatgcc atcaaatacc tgactcaatt tgatggaaac aacagttatt tctctacttc
     1621 tgtcattggt ttattgggcg attgttttgt agaaatcggt cagaatgata aagctttgac
     1681 ttatttcaac aaggctattg cagttgaaaa tgaggttttg agcccccttt atttgaaaaa
     1741 agccggtctg ctttgcgaat cgatgaatca acttaaaaag gccgaagaat attttcttga
     1801 aattaaggaa aaatatccaa aaagtatgga ggcaggcgac atcgacaaat atctggcacg
     1861 cgttcagaac taatttgacg ttttttgtcg ggtaaaattt gaaaaaattg tttttttaaa
     1921 aaaatatcat aaaaaattac attataaccc aatgtccaca gaatataaaa atctttcctc
     1981 ctataatgaa gatgaaatgc cggataaaag caaggttttt ctgcaacaat acggcattgt
     2041 ggtagctgat tggaatcctc aaatcaccca tgccatgttg gagggagctt ataatgcctt
     2101 gatagaaaac ggctcaaatc ccgaattaat caatattgtt catgtaccgg gtacttttga
     2161 gctgacttat ggcgctaaac agctgttgga gcaatatgaa gaaatggacc atataaaacg
     2221 acacaaatat gcagctatca tagctttggg ttgtgtaatt caaggagaaa cgccccattt
     2281 taattatgtc tgcgaaggtg ttacaaacgg aattgcatat ttaaatgcca atgcagtagg
     2341 ttgtccggtg atttttggtg ttcttactac caaaacactc cagcaggctc tcgaccgtgc
     2401 cggaggaatc aatggcaaca aaggcgttga ggctgccatt accgctataa aaatggcaaa
     2461 tatcgattgg taaatatttt tctcaaccaa tattttacaa cttttccgca agcagcttgg
     2521 tcaatagagc attggctttc caagttgagg gcaggggttt gaggattgtt cttttttcct
     2581 ctaatgaaaa gcgaccattt actgatcgct ttttactttt tatcggatag tgaaaattgg
     2641 aattattcaa ttttcttttg gccgaattgt tttggtttga attttttgag gaggagctgc
     2701 atttttttca acaacctgct tcaaactatc ttgccactga ggaacaaaat agcgtaacaa
     2761 ccgaatgctg tcaagctgaa cattgaaatt tccattatat tgtttgcaac ccaacagcaa
     2821 gccggtaaca gaatcgactt tatagggaaa tctggcccga agtcggagcg tgtaacgacg
     2881 caatatgctg tcgttgaaag ttttttgaaa aatactgtcc gttttcccat tcgcataatg
     2941 aattttcatt actacataag ggtcaacaca ggaatcttca ggagcaattc tttccaaaaa
     3001 ttgaagttcg taaacatctt gtgtcatcag ataaggctgc tgaattttaa aaggcaattt
     3061 cgttcgagcc gagtccggag taaaccgata actcggatga agattccata aattactgat
     3121 ttctattccg ggtaaaatag aatcaggcga gcgaaacttc cccttaacga tgtcttctct
     3181 gagatgatta agattttgca gtacttcagc ataaatgcgt tcaaattttt tgggattttt
     3241 ggcataccaa accatagaag aatcaaattc tgcttttgta attccgtgtt ttttaagtaa
     3301 ggattcaaaa tacaaagtac cttcaggagt ataatattgg tttgttgtct gaagagtgcc
     3361 gtgcaacaaa tggtattcgg taagcatctt caccatttcc ttcttactca acacattttt
     3421 gggtcgatta tcgcaagccg gcagcagaaa aagcgcgagg agcaaaaaga aagaaaagat
     3481 tttatttgga ctgctcagca acattggttt tttctttttt ctcaaaactt gcatttaatt
     3541 cttttcgata gaaaatcaga ataataaaca cggaaattgt aatggcagca tcggcaacat
     3601 tgaaaacagg tcggaaaaat attgtctcac ctgcagcatt ttttataagc gggaaataaa
     3661 gcatgtcgac cacttttccg taaaatagcg gggcataacc gccttcggga ggtaaaaaag
     3721 tagccactct tccatagctg tcgctgaaaa ggacgccata gaaaacagag tcgaaaatat
     3781 ttcctgctgc gccggccaaa agcaatgaaa tggacaacac ataaccggta cgaaaatttc
     3841 gcttaattag cgatcgaatg tagaatatga tcaaaacaac tgcgccaatt ctgaaagcag
     3901 tcaggaaaag cttgtccaac aattcaatgc cgaaagccat tccgttgttt tcgataaaat
     3961 aaatctgaaa ccaactaaaa atatcgatat gatcgcctaa agcaaaatgc gttttgatgt
     4021 aaaatttgct ggcttgatct acaagcaaaa caaagattat caataaaacc gatttttttg
     4081 tagttgacat gaacaaaagc tgcttttatt aaaaaattgg caacaaagaa aaatactctg
     4141 ttgccgtctt taatagtgga tagaatatat aaattatttt ttcgactttt tggcttcaat
     4201 acttagtgta gcatgaggaa cggcacgcaa gcgttcttta gggatcagct tgccggtctc
     4261 gcgacaaatt ccgtatgttt tgttctcaat tctaacaaga gcagcctgca aatgctgaat
     4321 gaattggagc tgacgctgag ctaaacgtcc ggcttcttct tttgaaagtg tagatgcacc
     4381 ttcttccaaa accttaaagg ttggagaagt atcggttaca tcattcccat cactattggt
     4441 aacaccgttg cgtaataatt cgtaatcttt ttgagctttt tccaactttt cgaggataat
     4501 ctgacgaaat tcttccagct cttcatccga atatcttgtt ttttctgaca taatcgtaaa
     4561 actttatttt gtttaatccc tttgtttcgg ttcagcattc tgcaaatgct gagtttttaa
     4621 taatgtgcga aaataatcgt tttttttctc ttttccaatt attttagtaa taattttgtg
     4681 gctttttcag cgtgtttttt caactttaac cttcaccata tattcatcaa aatcaagctc
     4741 aatgccatct tccagtttat ctaccaaaga aatgccggca gccaatgttt gagaggaaat
     4801 atagttcgaa aattccgtaa cggcatcgtt gatttcatct cttttttcaa tttcaacagt
     4861 tattctgtcg gttatttcat agccattttg cttgcgtata ttttgaattc tgtttaccaa
     4921 ttcgcgcgca attccttctt tgagcaattc atccgtcacg gtaatatcca gcgcaacggt
     4981 aagtttccct tcattggcag taagccagcc cggcatatcc tctgttatta tttcgacgtc
     5041 atctgttgta agttgaactt ccccaccggg caaatttaaa atataaattc cattttgctc
     5101 cagttcggca atttctttgt tgctcaattt tgcggttgcc tctgcaattt ctttcatttg
     5161 tttgccgtat tttttaccca aaaccttaaa attgagtttt acctttttgg ttaaatttgc
     5221 catttccata ttggccggta tcacttgcaa ttcttttatg tttacttcgg ctttaattaa
     5281 atcggccaca taactaattt gctgatagat tttatcatcg gcagccggaa caatagcttt
     5341 agaaagtggc tgacgaactt tcttcccggc ttttcgacgc agagaaagaa tcatggagga
     5401 gatttgctga gccagttgca tacattcttc cagatttttg tcgattaact tttcgtcgta
     5461 aacgggaaaa ttgctcaaat gaacagattc gcaattattt ttttccgtaa cttcattcaa
     5521 gtcgagaaac aattggtcgg cataaaacgg agcgattgga gccattaatt tggcaacggt
     5581 ttccaaacag gtataaagcg tttgataagc cgagatttta tccttgttgt attctccacc
     5641 ccaaaatctt ttccgattca aacgaacata ccaattgctc agattttcgg taacaaaatc
     5701 ggaaattgct ctgcctgctc ttgttggatc gtaatcatcg taatattcac caacttcctt
     5761 tatcaatgta tttaacaatg atataatcca tcggtcgatt tccggacgat tttccaaagg
     5821 aatttcgggc tcgctgcaag taaattcatc tacattggca taaagagcaa agaaagaata
     5881 agtattataa agcgttccaa agaatttcct tctcacttct tccactcctt caatatcaaa
     5941 tttcaaattg tcccagggag cggaattggt catcatatac caccgcaagg ggtcagaacc
     6001 gtatttttcg atggtt
//
LOCUS       DAOSCH010000112         5903 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_8559_length_5903_cov_6.803181, whole genome shotgun sequence.
ACCESSION   DAOSCH010000112 DAOSCH010000000
VERSION     DAOSCH010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8559_length_5903_cov_6.803181"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1953)
                     /locus_tag="PLZ54_07775"
     CDS             complement(70..1953)
                     /locus_tag="PLZ54_07775"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOJ66858.1"
                     /translation="MEEIVEKVTLTEYNDSNIITLEGLEHIRRRPGMYIGKLGDGSHP
                     DDGIYVLLKEVMDNCIDEFRMGAGKTIEVQIVNDMVQVRDYGRGIPLGKMIEAVSIMN
                     TGGKYDSKAFKKSVGLNGVGTKAVNALSEKFIAQSFREGQTRKAAFEKGKLTFDSGIV
                     ESDAERGTLITFTPDHEMFDGYEYRDEVVETMLRNYVYLNAGLTIVYNGKKIISRNGL
                     TDLLNENMTVPALYPIIQLKGEDIEVAFTHCDQYGEEYYSFVNGQHTTQGGTHQSAFR
                     EAVAKTIKEFYSKNMEYTDIRNGIVAAIAVNIEEPVFESQTKTKLGSRDIAPGGISVN
                     KFISDFIKTELDNYLHRNLETAEILLQKIQDSEKERKAIAGVTKLARERSKKVSLHNK
                     KLRDCRVHYNESKPGAEESSIFITEGDSASGSITKSRDVNTQAVFSLRGKPLNCFGLT
                     KKVVYENEEFNLLQAALNIEDGIEGLRYNKIIVATDADVDGMHIRLLLITFFLQFFPE
                     LIKKGHVYILQTPLFRVRNKKEKIYCYSEEEKIAAVKRLGATAEITRFKGLGEISPDE
                     FKNFIGKDIRLEQVSLRREDAVSELLEFYMGKNTSERQNFIIENLVIEEDKVEEEIEL
                     KIE"
     gene            complement(2022..3221)
                     /locus_tag="PLZ54_07780"
     CDS             complement(2022..3221)
                     /locus_tag="PLZ54_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOJ66859.1"
                     /translation="MIIAFACSGTQLLAQNVKQLEEERKQTLQKLETTSKMLDETKKS
                     QKSSLNKLSLLNKNINERTHLIKTMSKEISQLDVEIKTMGQEIEQLERRLENLKNDYA
                     RLVQEAYINRSIYSKIMFVLSAETFDQSLRRIRYLKEYSDYRKHQVSEIEKVKAQIEQ
                     KKDSISKHKSTLVVVVKEKEVEATKLSQEQKKEKKLLTDLQKKEKNLRKELDAQQKKA
                     NDLNKRIESLIAEEIRKAENLNKSKGKSTTSKVFTLTKEEVLLSGNFEKNAGRLPWPV
                     SNGFISGHYGIQPHPVLKHVTTNNKGIYIQTPANSTARAVFDGVVTQLFSVPGSNNAV
                     IIQHGEYRTVYANLTDIYVKIGDKVSTKQAVGKIFTDSEKDNKTELYFQIWKNKNLLD
                     PEKWLAR"
     gene            complement(3311..4108)
                     /locus_tag="PLZ54_07785"
     CDS             complement(3311..4108)
                     /locus_tag="PLZ54_07785"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOJ66860.1"
                     /translation="MKKILSIFLMILVLLSSCKTKKQVQQPSYFPQKLTTEKVIEQLN
                     ALDNIQTAEIKKMNIYLKMKDREFNVTSSMTIKKDSAINISIQPFFGMELFKLEITPN
                     NIFIFDKVNQTYYTTDFQYLNLRTGLGIDFYHLQALLTNRYFCLARQKDIACSILAQN
                     NHKISFGFDNETLMQTMSINPDFNITEILLVDKINNLSFNALYENFALINGIIFPRQI
                     TYMLKGQKHLSSAKITIDRVKFNQQVEFIPTDTRRYKKGSFDQLFKK"
     gene            complement(4153..>5903)
                     /locus_tag="PLZ54_07790"
     CDS             complement(4153..>5903)
                     /locus_tag="PLZ54_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ66861.1"
                     /translation="LFPILIFLCLLTISCSSTRKSVTREKNFRSNKLTPEQQSQFDYY
                     FYEALRLKNNQQLDEAMETFLFCRQIDSLDAEVQSQLGVLYANIGLIDPALKALKKSV
                     ELQPTNWWYSMTLLNIYSQQKNWKDAIALANEMQKIYPEKEEIYSTLAQLYKASGDID
                     KAIDAYNRLENLTGIDESISFAKFSLYAQQNKVKKAIAEIDRLIEKYPTENRYKVLRG
                     DIYLQLNNLEQAFAIYEQILKEEPQNPFVYISLSEYYKKINQPEKATEAIVSALKNEQ
                     LDVDTKIEILGQYIDNLIKDKEKLDETEALFKLLVDRYPLEEQVHAYYALFLEYQERI
                     PEAISELETMLNINPKNLQTWLKLIQIYWSDKNFEKIYVTTERAIEVLPEEPILYFYQ
                     GIALYQLNDYQASLQKNKKALLYIKDQQKALKSDVYAQLGDTYFKIGQKDSAFIAYEE
                     SLKANPENIYVMNNYAYYLSLEKKDLKKAERMSAKTVEKEPNNATFLDTYAWILYQQG
                     NYSLAKFYIERAIDNLKDGEDNGIIYEHYGDILWMYGGNDEKALEMWIKSYELGNQTE
                     ELKQKIENKGFDRQNK"
BASE COUNT         1522 a         1120 c         1127 g         2134 t
ORIGIN      
        1 aaccaatttt caattcgatt tttctacctg atgatgctaa tggacaattt gggataaata
       61 aaaaaggagt tattctattt ttaactcaat ttcttcttca actttatctt cttcaatgac
      121 cagattttcg ataataaaat tttggcgttc gctggtattt tttcccatat aaaattcgag
      181 caattctgaa acagcatctt ctctgcgtaa cgaaacctgc tctaagcgga tgtctttgcc
      241 tataaaattt ttaaattcgt cgggcgaaat ttcacccaag cctttaaagc gcgtaatttc
      301 ggcagtggct cccaatcgtt taacggctgc aattttttct tcttccgaat aacagtaaat
      361 tttttctttt ttgttccgaa ccctgaaaag cggtgtttgt agaatgtaaa cgtgcccttt
      421 tttaatcagt tcggggaaaa actgaaggaa aaaagtaata agcagcaagc gaatatgcat
      481 accatctaca tcggcatcgg tggcaacaat gattttgttg taacgaagtc cttcgatgcc
      541 gtcttctatg ttgagagcag cttgcaacag gttgaattcc tcgttttcgt acaccacttt
      601 tttggttaac ccgaaacagt tcagtggctt gccgcgaaga ctgaaaactg cctgcgtatt
      661 cacatcacgg ctttttgtaa tagagccgct tgcggaatcg ccttcggtaa tgaaaatgga
      721 cgattcttcg gcgccgggct tcgattcgtt gtaatgaacg cgacagtcgc gtaatttttt
      781 gttgtgcaaa ctgacttttt tggaacgttc acgtgccagt ttcgtaacgc cggctatggc
      841 ttttcgttct ttttccgaat cctgaatttt ttggagcagt atttcagctg tttcaaggtt
      901 gcgatgaagg taattgtcca attcggtttt aatgaaatcg gaaataaatt tattgacaga
      961 tattcctccc ggtgcaatgt cgcgcgaacc gagtttggtt ttggtttgag attcaaaaac
     1021 cggttcttct atgttgacgg caatggcagc cactattccg ttgcgaatgt cagtatattc
     1081 catgtttttg gaataaaatt ccttaatggt ttttgctacc gcttcgcgaa aagcactttg
     1141 atgggttccg ccttgcgtag tatgttgtcc gttaacaaaa gaataatact cttctccata
     1201 ttggtcgcaa tgggtaaaag caacctcaat atcttctcct ttcagttgaa tgatagggta
     1261 tagagccgga acagtcatgt tttcgttcag taaatcggtc aaaccgttac gggaaataat
     1321 ttttttcccg ttgtaaacaa tggtaagtcc cgcatttagg taaacgtaat ttcgaagcat
     1381 ggtttccacc acttcgtcgc ggtattcgta tccgtcgaac atttcgtggt cgggtgtgaa
     1441 ggtaatcaaa gttccgcgtt cggcatccga ttcaacgata ccgctgtcaa aagtaagttt
     1501 gcctttttca aatgcggctt ttctggtttg tccttctctg aagctttgcg caataaattt
     1561 ttccgagagc gcatttactg ctttcgttcc cacgccattt agtccaactg attttttaaa
     1621 ggctttggag tcgtattttc cgccggtgtt cataatcgaa accgcttcta tcatttttcc
     1681 caacggaata cctcgaccgt aatcgcgaac ctgtaccata tcgtttacga tctgaacttc
     1741 gattgttttt cccgctccca tacggaattc atcaatgcaa ttgtccatca cttcctttag
     1801 taggacataa attccatcgt cggggtgaga accgtctccg agttttccta tgtacatgcc
     1861 gggacggcga cggatatgtt ccagcccttc cagcgtaatg atattgctgt cgttatattc
     1921 tgtcaacgta actttttcaa ctatttcttc catatttcca tagaatagat tataaaaaaa
     1981 attcacttta aaccaatgtg caaatttaat tgatttttgg tttatcgtgc gagccatttt
     2041 tccggatcca acaaattttt atttttccat atttgaaaat aaagctccgt tttgttgtcc
     2101 ttttcgctgt cggtaaaaat tttacctact gcttgtttag tagaaacctt atcccctatt
     2161 ttgacataaa tgtcggtcaa gtttgcataa acggtacgat attcaccatg ttgtataatg
     2221 acggcattgt tgcttccagg tacggagaaa agttgagtaa ctacgccatc gaaaacagct
     2281 cttgccgtgc tattggcggg tgtttgaatg taaatacctt tgttgttggt tgtaacatgt
     2341 ttcaatacgg ggtgaggctg aatgccataa tgtccgctga taaaaccgtt gctgacaggc
     2401 cagggaagcc gtcccgcatt tttctcgaaa ttgcccgaca ataaaacttc ttcctttgtt
     2461 agtgtaaaaa ctttgcttgt tgtcgatttt cctttacttt tattaagatt ttcggctttt
     2521 cgaatttctt cggctatgag ggattctatt cgtttgttta aatcgttggc ttttttttgc
     2581 tgtgcatcca attccttacg taaatttttc tcttttttct gaagatctgt caacagtttt
     2641 ttttcttttt tttgttcctg cgacagcttt gttgcttcta cttctttttc tttcaccact
     2701 actaccagtg tgcttttgtg tttggatata ctgtcttttt tttgttcaat ctgtgcttta
     2761 accttttcta tttcgctaac ttgatgcttc cgatagtcgc tgtattcctt taaatatcga
     2821 atgcgccgta gggattgatc gaaagtttct gccgaaagca caaacataat tttggaataa
     2881 atactgcggt tgatatatgc ttcctgtacc agccttgcat aatcattttt taaattttcc
     2941 aatcgacgtt ccaattgttc gatctcttgt cccattgttt ttatttctac atcaagctgc
     3001 gaaatttcct tactcattgt tttaatcaga tgagtacgtt cgttgatgtt tttattgagg
     3061 agagaaagct tgttcaatga acttttctgc gattttttgg tttcgtccag cattttgctg
     3121 gtcgtttcca atttttgaag cgtttgcttg cgttcttctt ccaattgttt tacgttctgg
     3181 gctaagagtt gcgtccctga acaggcaaaa gcaattatca aaaaaagctg gattaaataa
     3241 gtacgaaaca tcttgacaaa ataaaaaaaa ttatataaaa aatgaatagc aaattatgag
     3301 gctgaattgt ttattttttg aacaactgat caaaacttcc ttttttataa cgacgggtat
     3361 cggttgggat aaattcaact tgctgattaa atttcactct atcaatggta atttttgcgc
     3421 tggataaatg tttttgtcct ttcaacatat atgtaatttg tcttggaaaa attatcccat
     3481 ttatcaaagc aaaattttcg tataatgcat tgaaagatag gttattaatt ttatcgacca
     3541 gtaaaatttc tgttatattg aaatcggggt taatgctcat ggtttgcatc agtgtttcgt
     3601 tatcgaatcc aaagcttatc ttgtggttat tttgtgcaag aatgctgcat gcaatatctt
     3661 tctgtcgagc taaacaaaaa tagcgattgg tcagcagggc ttgcagatgg taaaaatcaa
     3721 taccaagccc agtccgaaga ttcagatact gaaaatctgt tgtataatag gtttggttaa
     3781 ctttgtcgaa tataaaaata ttatttggtg ttatttccag tttgaaaagc tccatgccaa
     3841 aaaaaggctg aatggaaata ttgatagcag agtctttttt tattgtcatt gaagaagtta
     3901 cattaaattc ccgatctttc attttcaggt aaatgttcat ttttttgatt tctgcagttt
     3961 gtatattgtc caatgcattc aactgctcga taaccttttc ggtagttagc ttttgtggaa
     4021 aataagaggg ttgctgaacc tgcttttttg ttttgcagga actgagcaat accaatatca
     4081 tcaggaaaat acttaaaatt tttttcataa acttgtaact tttggaagca aaaaattttt
     4141 ctgcaaattt tgttatttgt tttgacgatc gaaacctttg ttttcaattt tttgtttcaa
     4201 ttcctccgtt tgatttccga gttcgtatga ttttatccac atttccagtg ctttttcgtc
     4261 atttcctcca tacatccaga gtatgtcgcc ataatgttca taaattatgc cgttgtcctc
     4321 tccatctttc aagttgtcga tagcccgttc gatgtaaaat tttgccagcg aataattacc
     4381 ctgctggtac aaaatccatg cataagtatc gagaaaagtg gcattgttgg gttctttttc
     4441 cactgttttg gcactcattc tttcggcttt tttcaaatct ttcttttcca gcgacagata
     4501 gtacgcataa ttattcatca cgtagatatt ctcgggattg gctttcagcg attcttcgta
     4561 agcaatgaat gccgaatctt tttgaccgat tttaaaataa gtatctccca gttgtgcata
     4621 tacatcgctc ttcagcgctt tctgttggtc ttttatataa agcagagctt ttttattttt
     4681 ttgcagtgaa gcttgataat catttaattg atacaaagca attccttgat aaaagtacaa
     4741 tatgggttcc tcgggcaata cttcgatggc gcgttcagta gtaacatata ttttttcaaa
     4801 atttttgtcg ctccaatata tttgaataag ttttaaccat gtttgcaggt ttttagggtt
     4861 gatgtttaac atggtttcaa gctcagatat ggcttccggg atacgctcct ggtattccag
     4921 aaacaaagcg tagtaggcat gcacctgctc ttcgagcgga taacgatcga ccaagagttt
     4981 gaaaagtgct tccgtttcgt cgagtttttc tttgtctttt atcaggttgt caatgtactg
     5041 ccccagaatt tctattttgg tatccacatc caactgttcg ttttttaatg cgctgacaat
     5101 agcctccgta gctttttcgg gctgattgat tttcttgtaa tattccgaaa gggaaatata
     5161 gacaaaagga ttttgtggtt cttcctttaa tatttgttca taaatagcaa aagcctgttc
     5221 gagattgttt agttgaaggt aaatatcgcc tcgcaaaacc ttgtagcgat tctcggtcgg
     5281 atatttttcg atcaacctgt cgatttcggc tattgctttt ttaactttgt tttgttgcgc
     5341 ataaaggcta aattttgcaa aggaaatgct ttcatctatg ccggttaggt tttcgagccg
     5401 gttgtaggca tcaatggctt tgtcgatgtc gccggaggct ttgtacaact gagcaagcgt
     5461 ggaatagatt tcctcttttt caggataaat tttttgcatc tcattggcca aagcaatagc
     5521 atctttccaa tttttctgct gagagtaaat gttgagcagc gtcatgctgt accaccagtt
     5581 ggtaggttgc aattcaaccg attttttcaa tgctttcagg gcagggtcaa tcagcccgat
     5641 attggcatat agtacaccca gttgcgattg tacttctgca tccagcgaat cgatttgtcg
     5701 gcaaaagaga aaagtttcca tcgcttcatc gagctgctga ttatttttga gtcgtagcgc
     5761 ttcgtaaaaa taatagtcga actgactttg ctgttcgggt gtcagtttat ttgaccgaaa
     5821 atttttctct cgtgtaaccg atttccttgt cgatgagcag gaaattgtga gaagacagag
     5881 aaaaattaat atggggaata aaa
//
LOCUS       DAOSCH010000113         5865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_8632_length_5865_cov_5.232358, whole genome shotgun sequence.
ACCESSION   DAOSCH010000113 DAOSCH010000000
VERSION     DAOSCH010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8632_length_5865_cov_5.232358"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLZ54_07795"
     CDS             <1..250
                     /locus_tag="PLZ54_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ66862.1"
                     /translation="YEKGEPEEASQIKVFSSKSHIVIDNLSAENGEALIYDMAGRKVG
                     QEKFFSETITMFGDYPTGVYVVKAVSNKETVTKRIIVQ"
     gene            263..916
                     /locus_tag="PLZ54_07800"
     CDS             263..916
                     /locus_tag="PLZ54_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66863.1"
                     /translation="MKKEEIKLSLILVVGGLGMIFCLFFLDIPFLPNLKYKSEVLDIQ
                     KYGRPFDRSSYQPHPLPKSSYKSNRTYSSDELMTEQISADIPGRGFQSEANGYSFGNG
                     YTTVGSTFSQKLRSNTSSQGIGSSGGFYAAGKRNSSENGASGQASRSSMLAASELREP
                     FSNSTPAPQRGPYAPNQGGTDPGGDPTGPPIPVGEGWGVMLLLAAGYASFIKLRAKR"
     gene            1576..3885
                     /locus_tag="PLZ54_07805"
     CDS             1576..3885
                     /locus_tag="PLZ54_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HOJ66864.1"
                     /translation="MNRYLQHDPWCIIEEGFHPEKQLASESIFSLSNGHMGQRANFEE
                     YYSGETMLGSYVAGIYYPEKAERGSWKNGYSEFNDKIVNAPNWTVISVRLNDERLDIG
                     EWDLANFKRVLNMREGYLERTFEATSFKGHRIQVTVKRFLSMAENEIGVISYTVKSLN
                     YDGRISFLPLIDGDVRNYYTNYNEPFWNVLQTRTEQDVSHLWAQTRKLDFHVCCALTY
                     DFYKNRELLNVNPIKIEKEKVAGFSVGTDIKKGETVTLRKYVSVISSLNHPREELTER
                     ACSLARQAKNKGWDELFEEHKSVWAEKWRQSDVIIDNDIAAQQAIRFNIFQLNQTYSG
                     NDPRLNISPKGFTGEKFSGATRWDTEAVCVPFYLSTSPQNVARNLLLYRFRHLPKAIQ
                     NAEKLGFTNGAALFPAATFNGSEILNEWELSFEEVYRNGIIAYAIYNYVRYTNDLDYL
                     VNYGLKVLIAISRFWSQRVNISGQRKKYVILGVTGPNMYENNVNNNWFTNFIAVWCLN
                     YTMECIETIRKTNFRAFEDVAQSIGFSNEETFLWKDIVENMYFPEDKNLDIFLQQDGF
                     MDKTLTRANEIPESQRPINQHWTWDRILRSSLIKQADVVLGLYLFENFFDKETISKNF
                     DFYEPLTVHESSLSPCIHSIVACKIGKIEKGYELYLRTARLDLDDYNNEVAEGLHITS
                     MAGTWLSIVQGFGGMRIKEGLLHFYPQIPKQWQSFAFNVIFRGVHLNVRIEKSKVIIQ
                     HIKGEAIRLMIYDKSFMLLPESLIETPFE"
     gene            3926..5770
                     /locus_tag="PLZ54_07810"
     CDS             3926..5770
                     /locus_tag="PLZ54_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOJ66865.1"
                     /translation="MKKILFLLSFLLACFASKSADKKWVDHLEPTFWWTGMNQTQLQL
                     MFHGKDVALCDFSIQDKEVTIIRKELTDNPNYVFLYIDLQKVVSPKKFQIVLKKDKKI
                     QQYWYELKERKANSSLRQSFTSADAVYLLMPDRFCNGNTANDSVKGYFEGVHRAKPGA
                     RHGGDIEGIISKIPYLADLGITALWTTPLFEDNDTQYSYHHYACTDYYKIDPRLGNNT
                     DYLRLADTCHAYGLKLLMDVVPNHCGWKHWWMKDIPDKNWFNTWNNYTSSNYRITAWT
                     DPHASESDRERLIKGWFAPNMPDLNLDFPLLFDYLRQAYVFWIEYAGIDGMRVDTYPY
                     NNIKAASQFIQSILEEYPNMNVVGECWVKTPAETAYYQSGNRNADGFDSHLSSVMDFC
                     LKDVFSLAFNEKEGWENGIVRFYSHFAQDFVYPNPNFIMIFLDNHDIDRFQTAVSNDI
                     AKYKMALAMLLTLRGYPQIYYGTEIMMPGIPGSYEGHRFDFPGGWATDQRNAFTENGR
                     TTEENEIFNYLRYLLHYRKNNTVLQTGKMKQFIPENGIYVYFRYNEEKTVMIVANNNE
                     KPAELDLQRFNEMLKQYGSAKEIFSGNFYNLKNAFSIPAKSVMIYELQ"
BASE COUNT         1850 a         1095 c         1194 g         1726 t
ORIGIN      
        1 ttacgaaaaa ggagaaccgg aagaggcatc gcaaatcaag gtattttctt ccaaaagcca
       61 tattgtgatc gacaatctga gtgcagaaaa tggagaagca ttgatttacg atatggcggg
      121 acgaaaagtg ggtcaggaga aattctttag cgaaaccatt accatgtttg gcgattaccc
      181 aacgggagtt tatgtggtaa aagccgtttc aaataaggaa acggtaacaa aacgaatcat
      241 tgttcagtaa aagcggagag aaatgaaaaa ggaagaaata aaactgtcgt tgatattagt
      301 ggttggaggg ctgggaatga ttttttgcct tttctttctg gatattccct ttttgcccaa
      361 cttgaaatac aagtctgagg tgctggatat acaaaaatat ggcagacctt tcgatcgttc
      421 ttcctatcaa cctcatccac ttccaaaatc aagctataaa agtaatagaa cttattcttc
      481 tgatgaatta atgaccgaac aaataagtgc cgatattccg ggcagaggtt ttcagagcga
      541 agcaaatgga tacagcttcg gcaatggata cacaacagtg ggttccacat tttcccaaaa
      601 actcagatca aacacttcct cacaaggaat aggcagcagt ggtggtttct acgccgcagg
      661 aaaaagaaat tcttctgaaa acggagcttc gggacaagct tcgagaagta gtatgcttgc
      721 tgccagcgag ttgagagaac cgttttcaaa cagtacgccg gctccacaaa gaggtcctta
      781 tgcaccaaac cagggtggta cagatccggg cggcgatccc acgggaccac ccataccggt
      841 tggtgaagga tggggagtga tgttgctgct ggctgccggt tatgcttctt ttataaagtt
      901 aagagcaaaa agatagtttt tgtttgaatg ttcgaatatc aagctccaac atacctatcc
      961 cgaaattttt tctttctatt tcgatttcaa atcgtttttt aaaaattttt ccaactcgcc
     1021 tgtataataa tgtgtttgta aaaaaataaa gttgcgctct cctaaatatt tttaagggca
     1081 tcaagtttta ctttttaatt ttgtctactt gtgttgttta aaaaaaattc taactgaaca
     1141 cagaatttta tataattaca ttttgattga actttttcct gttttgaaat cgaaattttg
     1201 ctttcaaaaa attttcatta tatcaattgc cttatcttac tctgttttta aatttttttg
     1261 ataaatcttt ttcgtatgaa acccacatgt attacataca caaacacgaa taatgaatgt
     1321 aatattcaga catgtgggtt ccatcagacc attaaaaaat aaattatatt ctgatgaaat
     1381 attgtttttt tctctgaaaa atcgaataat tgaaatatat ttgcattact atataagttt
     1441 gacaaaatta gctaaaaatt ttcttgaaaa aatttgtgaa agcaaaaatg gaatagtcgt
     1501 gcggtttcta tttcaaatgt cgggtttaca ttatcagtgg aataaacaca taagcaaaca
     1561 cataattcaa ttgctatgaa cagatattta caacatgatc cttggtgcat tatcgaagaa
     1621 ggttttcatc cggagaaaca attggcttcc gaaagtattt tcagcttaag caatggtcac
     1681 atggggcaaa gggcaaattt tgaagaatat tattcgggcg aaaccatgtt gggttcttac
     1741 gtggccggca tttattatcc cgaaaaagct gagcggggaa gttggaaaaa cggctattcc
     1801 gaatttaacg acaaaattgt gaatgccccc aattggacag tcatcagtgt tcgtttaaat
     1861 gatgaaaggc tggacatagg cgaatgggac cttgccaact ttaaacgtgt gttgaacatg
     1921 cgcgaaggtt atctggaaag aacttttgaa gccacatcat tcaagggaca ccggattcag
     1981 gttactgtaa agcggttttt gagcatggct gaaaatgaaa tcggcgtcat cagttatacg
     2041 gttaagtctt tgaattatga tggccgtatt tcttttcttc ccttgataga tggagatgtg
     2101 cgaaactatt ataccaatta caacgaacct ttctggaatg ttcttcaaac cagaacggaa
     2161 caggatgttt ctcatttatg ggcacaaacc cgtaaattgg attttcatgt ttgttgtgcg
     2221 ctcacttacg atttttacaa aaatagagaa ttgctgaatg tcaatcccat taaaattgaa
     2281 aaggaaaaag ttgcaggttt tagcgtaggt acggacatta aaaaagggga aaccgttacg
     2341 ttgagaaaat atgtttcggt tatcagctcg ctgaatcatc cgcgcgaaga acttacggaa
     2401 cgtgcctgtt cccttgcccg acaggcaaaa aacaaaggct gggatgagct gtttgaagaa
     2461 cacaaatcgg tgtgggcaga aaaatggcgt cagtccgatg taattatcga taacgacata
     2521 gctgcacagc aagccatacg gtttaatatt tttcaactca accaaaccta cagcggaaat
     2581 gatccccgat tgaacatcag cccgaaaggt ttcacgggcg aaaaattttc aggagccaca
     2641 cgctgggata ccgaagcggt gtgcgtacct ttttatcttt ccacttcgcc gcagaatgtg
     2701 gcacgaaatc ttttacttta tcgtttccgg catttgccaa aagccattca gaatgccgaa
     2761 aaacttggtt tcacaaatgg cgcagccctt ttccctgctg caactttcaa tggcagcgaa
     2821 attctcaacg aatgggaact gtcttttgaa gaagtttatc gaaacggcat tatagcttat
     2881 gctatttaca attatgtgcg ctataccaac gacttggatt atcttgtcaa ttacggatta
     2941 aaggtactga ttgccatctc acgtttctgg agtcagcggg ttaatatttc gggacagaga
     3001 aaaaaatatg ttattttggg cgtaacagga ccaaacatgt acgaaaataa cgtaaacaac
     3061 aactggttta caaatttcat tgccgtttgg tgtttgaatt atactatgga atgtattgaa
     3121 accatacgaa aaaccaactt ccgtgctttt gaagatgtgg ctcaaagtat tggcttctcc
     3181 aatgaagaaa catttctgtg gaaagatata gtcgaaaaca tgtatttccc cgaagacaag
     3241 aatttggaca tttttctcca gcaagatggt ttcatggata agacactcac cagagccaat
     3301 gaaatacctg aaagtcagcg ccccatcaat caacattgga cgtgggatcg aatacttcgt
     3361 tcctcgctta tcaaacaagc tgatgtagta cttggacttt atctttttga aaattttttc
     3421 gataaagaaa cgataagcaa aaatttcgat ttttacgaac cgctcactgt acacgaatct
     3481 tccttatcgc cttgcatcca ttcgatagtg gcctgtaaaa ttggaaagat tgaaaaaggt
     3541 tatgaactgt atctgcgcac ggcacgactc gatttggacg attacaacaa tgaagttgcc
     3601 gaaggcctgc atattacaag tatggccgga acctggcttt ccattgttca aggttttggc
     3661 ggaatgcgca taaaagaagg ccttttgcat ttctatccgc aaattcctaa acagtggcaa
     3721 tcgtttgctt tcaatgtgat tttcagaggg gtacatttaa atgttcgtat cgaaaaatcg
     3781 aaagtaatca ttcaacatat caaaggtgaa gccatcaggc tgatgattta cgacaaatct
     3841 tttatgcttt tgccggaaag tttgattgaa acgccttttg aataagaaat attgaagtcc
     3901 attaaaaaat aatttgttta ccaacatgaa aaaaattctt ttcctgcttt cttttctttt
     3961 ggcttgtttc gcatcaaaat cagccgataa aaaatgggtt gaccatcttg aaccaacttt
     4021 ttggtggaca ggtatgaacc aaacacaact ccaactaatg tttcatggga aggatgtggc
     4081 actatgcgat ttttcgattc aggacaagga ggtaaccatt atccgtaaag aattgaccga
     4141 taacccaaat tatgtttttc tttatatcga cctgcaaaag gtggtttcgc caaaaaaatt
     4201 tcaaatcgtc ctgaaaaaag ataagaaaat tcagcaatac tggtacgaac tgaaagagcg
     4261 caaggccaat tcttccttgc ggcagagctt tacttcggca gatgctgttt atctgctgat
     4321 gcccgacaga ttttgcaatg gcaatacggc aaacgattct gtgaaaggat attttgaggg
     4381 tgtacatcgt gcaaagcccg gagcgcgtca tggtggcgac atcgaaggta ttatttccaa
     4441 aattccttat ctggccgatt tgggtatcac agctttgtgg actacgccgt tgttcgaaga
     4501 caacgatacg caatattcct atcatcatta cgcctgcacc gattattaca aaattgatcc
     4561 gcgtctggga aacaataccg attatttgcg actggccgac acttgtcatg cttatggatt
     4621 aaaattattg atggatgtag ttcccaatca ttgcggctgg aaacattggt ggatgaaaga
     4681 cattcccgat aaaaactggt ttaatacttg gaacaattat acctcttcca attaccgaat
     4741 tacggcatgg accgatcctc atgcttctga atccgatcgc gaaagactca taaaaggctg
     4801 gtttgctccc aatatgcccg atttaaacct tgattttccg cttcttttcg attacttgag
     4861 gcaagcttat gttttttgga tagaatatgc cggaattgac ggaatgcgtg tggatactta
     4921 tccgtacaac aacataaagg ctgcttcgca atttattcaa tcgattctcg aagaatatcc
     4981 caacatgaat gttgtgggcg aatgttgggt gaaaacgcct gcagaaacag cttattatca
     5041 atcgggcaac agaaatgcgg atggatttga ctcccatttg tcgagtgtaa tggatttttg
     5101 tctgaaagac gtgttttcct tagcctttaa cgaaaaagaa ggatgggaaa atggaatagt
     5161 tcgtttttac agtcattttg cgcaggattt tgtttatcca aatccgaatt tcatcatgat
     5221 ttttttggac aatcatgata ttgatcggtt ccaaacggcc gtcagtaatg atattgccaa
     5281 atacaaaatg gctttggcca tgttactgac actgcgcggt tatccacaaa tatattatgg
     5341 cacggaaatc atgatgcctg gcattccggg tagttacgaa ggtcatcggt tcgattttcc
     5401 cggtggctgg gctaccgacc aacgtaatgc ttttacagaa aatggcagaa cgacagaaga
     5461 aaacgaaatt ttcaactatt tgcgatattt gttgcattac cggaaaaaca atactgtact
     5521 tcaaaccgga aaaatgaaac agtttattcc tgaaaatgga atttatgtct atttccgtta
     5581 caatgaagaa aaaacggtta tgattgttgc caacaacaac gaaaagcccg cagaattgga
     5641 tctgcaaaga ttcaacgaaa tgttgaagca atatggcagc gctaaagaaa ttttcagcgg
     5701 aaatttttac aacttgaaaa acgctttttc cattccggca aaaagtgtga tgatttacga
     5761 acttcaataa acctgttacc aagttatgaa acccacatgt attgcataca caaacacgaa
     5821 tgaatataat attcagacat gtgggttcca tcagaccttt aaaaa
//
LOCUS       DAOSCH010000114         5735 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_8898_length_5735_cov_5.790317, whole genome shotgun sequence.
ACCESSION   DAOSCH010000114 DAOSCH010000000
VERSION     DAOSCH010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8898_length_5735_cov_5.790317"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(159..233)
                     /locus_tag="PLZ54_07815"
     tRNA            complement(159..233)
                     /locus_tag="PLZ54_07815"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(197..199),aa:His,seq:gtg)
     gene            345..1025
                     /locus_tag="PLZ54_07820"
     CDS             345..1025
                     /locus_tag="PLZ54_07820"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOJ66866.1"
                     /translation="MNKQTIEQNNEVKEILQKLWLSRNGEVASQMRRYGMVYRKNLGV
                     AITRIREIAFDYEPNEETAQQLWTLHGRETMLMAILLQPVNNFSWEMAQSWAQDCDNI
                     ETIEFACMHLFSKLPFVNELCCDWCASDKVQLQIMGFILSARNYQNFSDVDRNLIIEK
                     AFENSTTDNFHLYKAISLALCRFCRNGKPIADFILEKMSNFLPQPTAGQQYIMEEVKS
                     EMNFLELL"
     gene            1059..1532
                     /locus_tag="PLZ54_07825"
     CDS             1059..1532
                     /locus_tag="PLZ54_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOJ66867.1"
                     /translation="MDENKEKEENSFEVVKKIFNDYLAAHQHRKTPERYAILEQIYTH
                     EGHFNAELLYNAMQKDYRVSLATIYNTLDLLLECKLIVKHQFGDQLAQYEKTFGTNIH
                     HHLICTQCGKIKEFSDKNIKAAIQSKHFQTFQMSHYSLYIYGICSKCLKKKKKTS"
     gene            1657..2925
                     /locus_tag="PLZ54_07830"
     CDS             1657..2925
                     /locus_tag="PLZ54_07830"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOJ66868.1"
                     /translation="MKADVLLGLQWGDEGKGKVVDVLTPHYDIVARFQGGPNAGHTLE
                     FKGKKFVLRSVPSGIFQGDKINVIGNGMVIDPALFKEEVEALEAAGEPLHQRLKISKK
                     AHLILPTHRLLDLASEAAKGTGKIGTTGKGIGPAYTDKISRNGLRIGDILHRFDEKYR
                     EVVARHTEMLRQYNFEYDLAALEKTFFEGIECIKRFDLIDSEYFINHSLEEGKKILAE
                     GAQGTLLDIDFGSYPFVTSSNTICAGACTGLGLSPQKIGEVYGIFKAYCTRVGSGPFP
                     TELKDETGELIRKKGCEFGAVTGRPRRCGWIDLVALRYSIMLNGVTQLIMMKSDVLDD
                     FDTIKACVAYRIDGKETEEFPFEITENLEPVYVELPGWKTKISNLQSEDEFPEELNAY
                     LNFLEEELNVPIKIVSVGPDREQTVIRYQE"
     gene            3026..3703
                     /locus_tag="PLZ54_07835"
     CDS             3026..3703
                     /locus_tag="PLZ54_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOJ66869.1"
                     /translation="MIYIIFIVFALISFLLQNRLQSKFKRYSKLPVPNGMTGKDVAEK
                     MLHDNGIYDVKVISTSGHLTDHYNPANKTINLSEQVYASSNVAAAAIAAHETGHAVQH
                     AYAYAPLTLRSKLVPVVSFASMWMQWVLLAGIIFLQSFPQLMLTGIILFALTTLFSFI
                     TLPVEINASQRALAWLNNAGITNYETHEPAKDALKTAAYTYVVAALGSLATLVYYILI
                     FMGGSRR"
     gene            3811..3884
                     /locus_tag="PLZ54_07840"
     tRNA            3811..3884
                     /locus_tag="PLZ54_07840"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3845..3847,aa:Arg,seq:ccg)
     gene            4066..5472
                     /gene="rlmD"
                     /locus_tag="PLZ54_07845"
     CDS             4066..5472
                     /gene="rlmD"
                     /locus_tag="PLZ54_07845"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOJ66870.1"
                     /translation="MRKKKQLPVLEKVTITDIGAEGKALTKINDMVVFVPLVVPGDVV
                     DLQVTQKKKNYMEARAVKFHHYSEQRCEPFCQHFGICGGCKWQMLPYAEQLKYKQKQV
                     EDHLKHIGKVQLPEISPILPSEKTVFYRNKLEFTFSNRRWRSFEEISSAQNIEDENAL
                     GFHIPGHFDKVLNIEKCWLQDDISNKIRNEIRRYALENELEFFDLRHQEGFLRTMMVR
                     TSTTGEVMLVVVFFREDEPKRKALLQHIADTFPEITSLLYVINSKANDTITDQEIISF
                     TGNDSIYEKMEDLTFKIGAKSFFQTNSAQACRLYEKARDFAALTGNELVYDLYTGTGT
                     IANFVARKAKKVIGIEYVPEAVEDAVNNSKLNNITNTFFYAGDLKEVMTANFIREQGN
                     PDVIITDPPRAGMHGDVVDAILTTNAARIVYISCNSATQARDLQLLDSKYKVTAVQPV
                     DMFPHTHHVENVVLLEHR"
BASE COUNT         1797 a         1063 c         1172 g         1703 t
ORIGIN      
        1 taatttgtgg tgtaaaaatt tgaattttca ataagaaaat ctacagaatg actaaaaata
       61 cgtaaatttg cagaaagtgg ttatttttga atctcaatat tcttttcagc aattacttat
      121 tttaaacgca aaaaggcagc ctttttgctg ccctttgtgg gtggaggacg ggactcgaac
      181 ccgcgacatt cggaaccaca atccgacgct ctaccagctg agctacgacc accatttttt
      241 gcgagtgcaa agataacaca gcatttgatt ctgtgcaact attttaaaaa aaaattgacg
      301 tcttttggtt tattgccgat tttttggata aatgtttaaa tgaaatgaat aagcagacaa
      361 tcgaacaaaa caatgaggtt aaggagattt tacaaaaact ttggctttcc cgaaacggag
      421 aagtagcttc ccaaatgcga cggtacggga tggtgtaccg caaaaatctg ggcgtcgcga
      481 taacccgcat aagggaaata gctttcgatt atgagccaaa tgaagaaaca gcacaacaac
      541 tttggacact tcacggcaga gaaaccatgt tgatggcaat cctgcttcag cctgtaaaca
      601 attttagttg ggaaatggct caaagttggg cccaagactg cgacaacata gaaacgattg
      661 aatttgcgtg catgcatctt ttctcaaaac tcccttttgt caacgaactt tgctgtgatt
      721 ggtgcgcttc ggacaaagtt caattgcaaa taatgggatt tattttatcg gcacgcaatt
      781 atcaaaattt ttcggatgtg gatagaaatc ttattattga aaaggctttt gaaaattcga
      841 caaccgataa tttccatctg tataaagcca tcagtttggc gctttgcaga ttttgcagaa
      901 atggaaagcc catcgcggat tttattctcg aaaaaatgag caacttcctt cctcaaccaa
      961 ctgccggtca acaatatatc atggaagaag tgaaatcgga aatgaatttt ttggaacttt
     1021 tataacaatt atattctaaa aaatcttacc tttgttctat ggatgaaaac aaagaaaaag
     1081 aagaaaattc ctttgaagtg gtcaagaaaa tttttaacga ttatttggct gcccatcagc
     1141 atcgcaaaac gcccgaacgt tacgcaattc tggagcaaat ttacacccac gaaggacatt
     1201 tcaatgccga gctgctttac aatgccatgc aaaaggatta tcgtgtcagt ttggccacta
     1261 tttacaatac gctcgatttg ctgctcgagt gcaaattaat tgtaaaacat caatttggcg
     1321 atcaactggc acaatatgaa aaaactttcg gcaccaacat tcatcatcat cttatttgta
     1381 cgcaatgtgg caaaataaag gaattttccg acaaaaatat aaaagcagcc atccagtcga
     1441 agcattttca aacttttcaa atgtcgcatt actcgctgta tatttatgga atttgcagta
     1501 aatgcctgaa aaaaaagaaa aaaacttcgt aaaaagtgtt gtttcaatga aaaaaattac
     1561 cttatttttc accttttctg taaatggatg ccgtaagcaa acggattctg aagcaaggaa
     1621 aggttttatt ttgcaaaatc ataaaaattt aaacgaatga aagcagatgt tttattaggc
     1681 cttcagtggg gcgacgaagg aaaaggaaaa gttgtggatg tactgacgcc acattacgat
     1741 attgtggctc gatttcaagg cggccccaat gccggtcata ctttggagtt taaaggaaaa
     1801 aaatttgtat tgcgttcggt tccgtcggga atttttcagg gcgacaaaat caacgtgatt
     1861 ggcaacggga tggtgattga ccctgccctt ttcaaggaag aagtggaagc tctcgaagca
     1921 gcaggcgaac ccttgcatca gcggctgaaa atttccaaaa aagctcatct cattcttccg
     1981 acgcatcgat tgctcgatct ggcttccgaa gctgccaaag gaacaggcaa aattggtaca
     2041 acgggcaaag gtattggccc tgcttataca gacaaaataa gcagaaatgg acttcggata
     2101 ggtgatattc tgcatcgttt cgacgaaaaa tatcgtgaag tcgttgctcg ccatacggaa
     2161 atgttgcgtc aatacaattt tgaatacgat ttggctgcat tggaaaaaac tttttttgaa
     2221 ggaatagaat gtatcaaacg ttttgatttg attgacagcg aatactttat caaccactcg
     2281 ctggaagaag ggaaaaaaat tttggccgaa ggtgctcagg gaacgttgct cgacattgat
     2341 ttcggttcgt atccttttgt aacttcttcc aacacgattt gtgccggcgc ttgcaccggg
     2401 cttggcctct ctccgcaaaa aatcggagaa gtttacggta ttttcaaagc ttattgcacg
     2461 cgcgtgggaa gcggtccttt tccaaccgag ttgaaagacg aaaccggaga actgattcgc
     2521 aaaaaaggtt gtgagtttgg tgctgtaacc ggccgtcctc gtcgttgtgg ttggatcgat
     2581 ttggttgcat tgcgctattc aatcatgctc aatggcgtta ctcagttgat tatgatgaaa
     2641 agcgacgtgc tggatgattt tgataccata aaggcttgtg tggcatatcg tatcgacgga
     2701 aaggaaacgg aagaatttcc atttgaaatt accgaaaatc tcgaacccgt ttatgttgaa
     2761 ttgcccggtt ggaaaaccaa aatatcgaac ttgcaaagtg aagacgaatt tcccgaagaa
     2821 ttgaatgcct atttgaattt tttggaagaa gaattgaatg tcccgataaa gattgtttct
     2881 gtaggacccg accgtgaaca aaccgtcatt cgctatcagg aatagttttc caatttttat
     2941 gcactacaaa ctgccaatct gtcaattaaa tttttggcaa acattttgca tgaaataaaa
     3001 tgttaattat aaaattgaaa agactatgat ttatattatt tttattgttt ttgcgctgat
     3061 tagttttttg cttcagaaca ggttgcagtc aaaattcaaa cgttactcga aattacctgt
     3121 accaaatggt atgacgggaa aagatgtagc cgaaaaaatg ctgcacgaca acggcattta
     3181 tgatgtgaag gttatttcca cctccggtca tctcaccgat cattataatc cggcaaacaa
     3241 aaccattaat ttgagcgaac aggtgtacgc ctcgagtaat gtggcggcgg cggccattgc
     3301 tgctcatgaa acagggcacg cggttcagca cgcttatgct tatgctccac tgactctgcg
     3361 ttcaaaactg gttccggtgg taagttttgc ttccatgtgg atgcaatggg tactgttggc
     3421 aggtattatt tttcttcaat cttttcctca actgatgttg acgggaatta ttctttttgc
     3481 gctgaccacg cttttcagtt tcataacttt accggtagaa atcaatgcca gtcaacgtgc
     3541 gttggcctgg ctcaacaatg ccggcattac caattatgaa acccacgaac cggccaaaga
     3601 tgctttgaaa acggcagctt acacttatgt ggtagccgct ttgggatcgt tggcaacgct
     3661 ggtttattat atcctgattt tcatgggtgg aagccgaaga taaatataaa aattcataca
     3721 ttttgataag gaaaaccgac ttttttaaga gaaggttttc cttatttctt ttttcaaatc
     3781 gggaaaaatc actacatttg catccgcatt ggctccgtag ttcagttgta tagaacgtca
     3841 gattccggtt ctgaaggtct tgggttagaa tcccaacggg gtcacacaca gccaacctta
     3901 acatgtttgt tcgggttggc ttttttggta agaaaatttt tatttgaaaa taattgtatg
     3961 ttcttcactt tcagccgatt ggctcaaagt tgttacactt ccaatttttt cgttcaaaat
     4021 cactaatttt gcagaaaaat tttaatattg aatcctgctc aatcaatgag aaaaaagaaa
     4081 cagctccccg ttttggaaaa ggtaaccatt accgatattg gagccgaagg caaagcgctg
     4141 acaaaaataa acgatatggt cgtttttgtt cctttggtgg tgccgggcga tgtagtggac
     4201 ttgcaagtta cgcaaaagaa aaaaaattac atggaagccc gagctgtaaa atttcatcac
     4261 tattcggaac aacgttgtga gcctttttgt caacattttg gcatttgcgg cggctgcaag
     4321 tggcaaatgt taccttatgc agaacagttg aaatacaaac aaaaacaggt agaagatcat
     4381 ttgaagcaca ttggaaaagt tcaattgccg gaaatttcgc ccattctccc ttccgaaaaa
     4441 accgtttttt acagaaataa acttgaattt accttttcca atcgccgttg gcgaagtttt
     4501 gaagaaatat cttctgcaca gaatattgaa gatgaaaatg cactcggatt tcacatcccg
     4561 ggacatttcg acaaagtgtt gaacattgaa aaatgctggt tgcaggacga tatttccaat
     4621 aaaatcagaa acgaaattcg tcgttatgct ttggagaatg aattggaatt ctttgatctg
     4681 cgccatcagg aaggattttt gcgaacaatg atggtaagaa cttccaccac tggtgaagtg
     4741 atgctggtag tcgttttttt tcgggaagac gaaccgaaac gaaaagctct tttgcaacac
     4801 atagccgaca cttttcctga aattacttcc cttttgtatg taatcaattc caaagccaac
     4861 gataccatta ccgatcagga aataatttcc ttcactggaa atgatagtat ttacgaaaaa
     4921 atggaagatt taactttcaa aattggggca aagtccttct ttcaaaccaa ttctgcacaa
     4981 gcttgcaggt tgtatgaaaa agcccgcgac tttgcagcat tgacgggaaa tgaacttgtt
     5041 tacgatttat acaccggtac gggcaccata gccaattttg tggcgcgaaa ggcaaagaaa
     5101 gtgattggaa tagaatatgt acctgaagca gtggaagatg ccgtgaataa ttcaaaactt
     5161 aacaacatta ccaatacatt tttttatgcg ggtgatctga aagaagtaat gacagccaat
     5221 tttattcgtg agcagggaaa tcctgatgtc attatcactg atccgccaag agccggaatg
     5281 catggggatg tagtggatgc cattctgaca accaatgccg caagaattgt ttatatcagt
     5341 tgcaattcgg caacacaagc ccgtgatttg caacttttgg acagcaaata taaagttact
     5401 gccgttcagc cggtagatat gtttcctcat acgcatcatg ttgaaaatgt agtattgctt
     5461 gaacatcggt aaacagaata ttttaaatgt atttgttttg tacgagcctt tggaatcttt
     5521 tgttatcatt ataaccaatt gttaatcagt tataaagata caaaatttca aggtgttcac
     5581 caaaaatgac aattatttta ttgattatca gataattata taactataat ataatttttc
     5641 tcatgtgcta aaatatattt gaaaaaactt atagcttaaa ttttaaatta tcatgattag
     5701 tttttttatt tccattggac ttttaatagc aggtt
//
LOCUS       DAOSCH010000115         5697 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_8969_length_5697_cov_5.459943, whole genome shotgun sequence.
ACCESSION   DAOSCH010000115 DAOSCH010000000
VERSION     DAOSCH010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8969_length_5697_cov_5.459943"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(291..1724)
                     /locus_tag="PLZ54_07850"
     CDS             complement(291..1724)
                     /locus_tag="PLZ54_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ66871.1"
                     /translation="MSNFRWSIVTLLFFATAINYLDRQVLSLTWKDFIAPEFHWTNTH
                     YGTITSLFSLFYAIGMLFAGRFIDKLDTKKGFLWAIGIWSFGAIMHAFCGIATSGIAA
                     GKWLVGFEGAKEAIATVNNVSMVLNVSVTLFIFARLVLAIGESGNFPASIKAVAEYFP
                     KKDRAFATSIFNAGSTVGALAAPVVIPVIAEKWGWEMSFIIIGALGFIWMGFWAFIYK
                     KPEVHPKVNKLELEYIHLDHADEAAVNPNANTDKEKKLSFKQCFKFKQTWAFAFGKFM
                     TDGVWWFYLFWTPAYLSSVYGLKSSDPRSQLAIFVLYAITLLSIIGGWLPTYFVDKKG
                     MHPYAGRMKAMLIFAFFPLLALFAQPLGHISYWLPIIIIGIAGAAHQSWSANIFSTVS
                     DMFPKSAVATVTGIGGMAGGIGSMLINKASGALFDFAGQTQMSFLIFKGEEAGYFIVF
                     SFCAVAYLIGWIVMKALVPKYSPITDL"
     gene            complement(1802..2479)
                     /locus_tag="PLZ54_07855"
     CDS             complement(1802..2479)
                     /locus_tag="PLZ54_07855"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HOJ66872.1"
                     /translation="MMKFNKLEVLSTIKSTGVVPVFYHSNFEIAKSVVKACYEGGIRA
                     FEFTNRGEFAHEVFGQVVKFVQKDCPDMIMGVGSVIDAPTAALYIQLGANFVVGPLFN
                     PDVAKICNRRLVPYMPGCGSVSEIGFAQEAGCDVCKVFPGEVLGPAYVKALKAPMPWS
                     NLMVTGGVKPEEANLKSWFDAGVMCVGMGSNLFPADVIAAHDWGKITELCRETLDLIK
                     KIQKNIK"
     gene            complement(2523..3566)
                     /locus_tag="PLZ54_07860"
     CDS             complement(2523..3566)
                     /locus_tag="PLZ54_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HOJ66873.1"
                     /translation="MSKKIVTFGEVMLRLATPGYERFSQATEFSATFGGGEANVAVSL
                     ANYGMETEFVTRLPQNEIAESCIMDLRKHNVGTHYILRGGERMGIYFLETGAVARGSK
                     VIYDRAHSSIAEIEPGMIDWREVLKDADWFHWTGITPAISQGAADVCIEAIKVANELG
                     VTVSTDLNYRKNLWKYGKSASEIMPALVEGCDIILGNEEDAEKVFGIKPEGFDVSRTG
                     GEIDSAEFESVCKQLMKKFPRAKKVIITLRGSINANHNTWGGVLYSGEKLYQSKRYDI
                     THIVDRVGGGDSFMGGLIYGLLTYEGNDQKALDFAVAASCLKHTIYGDYNLVTVKEVE
                     ALMSGDESGRVSR"
     gene            3968..5044
                     /locus_tag="PLZ54_07865"
     CDS             3968..5044
                     /locus_tag="PLZ54_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOJ66874.1"
                     /translation="MEKAKGKIRIKDIARMAGVSEGTVDRVLHQRGVVSEKSRKAVKK
                     VLEEIDYTPNLLARSLASKKKYRFICLFPAYNKGEYWESIEKGFRQASEEFNNYKVEV
                     MIEYFDQFDSSSFLATAKAVFVYNPDAIIMAPVFRQETILFARELTEKKIPFSFIDSM
                     VENVEFTSYYGQHSLQSGYVAGKLLFNSLPNESKILVLHTYRKGGSGSIQTENRKKGF
                     LQYIEENNLKNFCEILSIEIIEGNDEENMRLIRNAFIQHPDIRAAITFNSKVHRLVKY
                     FKNLSHSDIKLIGYDVLEENVKCMKEGFVSFLIAQRPEKQAYCAVRDMSFHLIFDKEV
                     NKINYVPIDILMKENIDYYLNFNE"
     gene            5220..>5697
                     /locus_tag="PLZ54_07870"
     CDS             5220..>5697
                     /locus_tag="PLZ54_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOJ66875.1"
                     /translation="MNTSITSDWIQPALYQQPLENGMVKCVLCPHQCMVHEGKTGLCH
                     TRQNINGVLYTLAYANPCSLNVDPIEKKPLFHFLPGSKIFSLATAGCNFKCLNCQNWE
                     ISQSSPQEVIHYDLLPEAVVELASNRQIASIAFTYTEPTVFYEYVYDTAKLAHQKNM"
BASE COUNT         1825 a         1200 c         1000 g         1672 t
ORIGIN      
        1 tactgtgaaa aaatcgaacg ataatttcag aatggatgaa agttttccgt ttccaaaccc
       61 agtgaaatag tctcttttta gtactataga cagtactata tagaaaatca gactgccata
      121 gtagtgatat gtttttaaaa aattttacgg taaagcatta tcgctttgtc ttctgtccga
      181 cattcaaccg gacagaagac aaagcgataa acttgttaaa agaatcattg agcttacttt
      241 aattgtcgct tgactcttta tctaaagaat ttttgtcgtc tcccaactga ttataaatcg
      301 gttatgggag aatatttggg aaccaaagct ttcataacta tccaaccaat gagataggca
      361 acagcacaga aagaaaaaac gataaagtaa cccgcttctt cgcccttaaa aatcaaaaat
      421 gacatttgtg tttgccccgc aaagtcaaac aaagcacctg aagccttatt aattaacatt
      481 gaacctatac ctcctgccat tccgcctatg ccggtaactg tagccacagc acttttagga
      541 aacatatcgc ttacggtaga aaaaatatta gccgaccatg attgatgtgc agctcccgct
      601 atacctatga taataatggg caaccaataa gaaatatgac ccaatggctg ggcaaaaagg
      661 gccaacaatg ggaagaaagc aaaaatcaac atggctttca ttcttccagc atagggatgc
      721 attccttttt tatcgacaaa ataggtcggg agccagcctc caataatgga caaaagtgta
      781 attgcataaa gaacaaatat agccagctga cttcttggat cggatgattt cagtccgtac
      841 acagaactca aatatgcagg agtccagaaa agatagaacc accaaacccc gtctgtcatg
      901 aattttccga aagcaaaagc ccatgtctgc ttaaatttaa aacactgttt gaaactcagt
      961 ttcttttcct tatccgtatt ggcattagga ttcacagctg cttcatccgc atgatccaaa
     1021 tgaatgtact ccaattcgag tttattcact tttggatgca cttctggttt tttgtaaata
     1081 aatgcccaaa accccatcca aataaaaccc aaagctccga taatgataaa cgacatttcc
     1141 caaccccact tttctgcaat cacaggaatg acaaccggtg cagccagtgc tccaaccgta
     1201 gaacctgcat taaagatact ggtggcaaaa gcacggtctt ttttcgggaa atattctgct
     1261 acagccttga tagaagccgg aaagttaccc gattctccga tggcaagtac caaacgcgca
     1321 aaaatgaata aagttacact cacattcagc accatcgaaa cattattcac tgtagcaata
     1381 gcttcctttg ctccctcaaa accaaccaac catttacctg ctgcaatgcc agaagtggca
     1441 ataccacaaa aggcatgcat tattgcgcca aaagaccata ttccaattgc ccacagaaaa
     1501 cctttttttg tatccaactt atcaataaat cggcctgcaa acaacatccc aatggcataa
     1561 aataacgaaa ataaagaagt aatggtacca taatgagtat tggtccaatg aaattcagga
     1621 gctatgaaat ccttccacgt cagcgaaaga acttgcctgt ctaaataatt gatcgccgta
     1681 gcaaaaaaca atagcgtgac gattgaccac ctgaaatttg acattttttt agcttgatgt
     1741 tccatttcaa ttgttttacg atattagaag ttttacaatg tttgccttat gaagcaaata
     1801 attatttgat gtttttttga atttttttta tcaagtctaa tgtctccctg caaagttcag
     1861 ttatttttcc ccaatcatgg gcagcaatca catccgcagg aaacaaattg gaacccatcc
     1921 ccacacacat gacaccggca tcaaaccagc ttttcaaatt tgcctcttcg ggttttactc
     1981 cgcctgtaac cattaaattc gaccagggca tgggcgcttt caatgccttt acataggccg
     2041 ggcccaagac ttcgccgggg aaaaccttgc aaacatcaca accggcttcc tgagcaaatc
     2101 caatttccga aaccgatcca catccgggca tatagggcac caaacgccga ttgcaaattt
     2161 tagcaacatc gggattgaac aaaggaccta ccacaaaatt ggctcccaac tgaatataaa
     2221 gcgctgctgt tggagcatcg atcaccgaac ctacgcccat aatcatgtcg gggcagtctt
     2281 tttgaacaaa tttaaccact tggccgaaaa cctcgtgcgc aaattctcct ctgttggtaa
     2341 attcaaatgc tctgattccg ccctcgtaac aagctttgac cacgcttttg gcaatttcga
     2401 aattgctgtg gtaaaacacc ggcaccactc cggtggattt tatggtagaa agtacttcca
     2461 atttattgaa tttcatgatt ttatttggta ttttaatatc ctgattgtaa aattttaaaa
     2521 tgttaccggg aaactcttcc gctttcatct ccgctcatca aagcttccac ttcctttacg
     2581 gtaaccaggt tataatcgcc gtaaatggta tgtttcaggc aagaggctgc cacagcaaaa
     2641 tccagcgcct tctgatcgtt gccttcgtaa gtcaacaatc cgtaaatcag tccgcccatg
     2701 aaagaatcgc cgccgcccac tctgtccaca atatgcgtta tgtcgtaacg tttcgattga
     2761 tagagttttt caccactgta gagcacgcca ccccatgtat tgtgattggc attaatggat
     2821 ccgcgcaaag taatgatcac ttttttggca cgtggaaatt ttttcattaa ttgcttgcaa
     2881 accgattcaa attcggcaga atcaatttct ccgccggtac gggagacatc gaaaccttcc
     2941 ggcttgatgc caaaaacttt ttccgcatct tcctcatttc cgagaataat atcgcaacct
     3001 tcaaccagtg cgggcattat ttcggaagcg gattttccgt atttccaaag attttttcta
     3061 taattcaaat ccgtagatac cgttacgccc agttcattgg ccacttttat ggcttcgata
     3121 catacgtcgg cagctccctg cgaaatggcc ggcgtaatgc ctgtccagtg aaaccaatcg
     3181 gcatctttca atacttctcg ccaatcaatc atgcccggct caatttccgc aatggacgaa
     3241 tgtgccctat cataaattac cttgcttcct cgtgctaccg ctcccgtttc caagaaataa
     3301 attcccatgc gttcgccgcc acgtaaaatg taatgcgtgc cgacattgtg cttgcgcaag
     3361 tccatgatgc atgattcggc aatttcgttt tgtggcagcc gcgtaacaaa ttcggtctcc
     3421 attccgtaat tggccaacga aaccgccaca ttggcttctc ctccgccaaa agtggcacta
     3481 aactccgtag cctgactaaa tctttcatat cccggcgtgg ccaaccgtag cattacttct
     3541 ccaaatgtta caattttttt gctcattttt cagtctcctt tttaattgtt gataaataat
     3601 tggtcgacca aaattactgc ttttatattc actttcaaaa tttttttatt tcgttttcaa
     3661 aaaaaatttt tttccttgaa aatctgcgga aaaaataaaa agaagacgaa atttttgaaa
     3721 ttagttttat taactttgct caattaatta taagataaaa atccggcgaa aaacaagaac
     3781 aagtgctaaa atttcttata ttgccataaa gtaaaaagca aatcatgaat tgaaaatcag
     3841 gtttcaatga aaattctgtg acttgttggc aaaagtacaa aacaatttca tattatcaaa
     3901 tatttatttt aattttggac atattttttt tgtgcacgta cacaaaataa caattaaaaa
     3961 gcaagatatg gaaaaagcga aaggcaaaat acgcattaag gatattgcca ggatggcagg
     4021 agtatctgaa ggtacagttg acagggtgtt gcatcagcga ggtgtagttt ccgaaaaaag
     4081 tcgaaaagca gtaaagaaag tattggaaga aattgattat acaccaaatt tgcttgctcg
     4141 gtcgttggct tcgaaaaaaa aatatcgttt catttgtctt ttccctgcct ataacaaagg
     4201 tgaatactgg gaaagtatag aaaagggatt tcgacaagcc tctgaagaat ttaacaatta
     4261 caaagtagag gtaatgattg aatattttga tcagtttgat agcagctcat ttttagcaac
     4321 ggcaaaagct gtttttgtct ataatcctga tgccattatt atggctccag ttttcaggca
     4381 agaaaccatt ttatttgcca gagagcttac agaaaaaaag atcccttttt cttttatcga
     4441 ttctatggta gaaaatgttg aatttacatc ttattacggt cagcattctt tgcaaagcgg
     4501 atatgttgca ggcaaattac tgtttaattc tttaccgaac gaaagcaaaa ttttagtact
     4561 tcatacttac agaaaaggtg gttctgggtc tattcagacg gaaaatagaa aaaaaggatt
     4621 tttgcaatat attgaagaaa ataatttgaa aaatttctgt gaaatattgt ctatagaaat
     4681 tattgaggga aatgatgaag aaaacatgcg cttaatccgc aatgctttta tccaacaccc
     4741 ggatattcga gctgccataa cttttaactc taaagttcat agattggtca aatatttcaa
     4801 aaacctttct cattcggaca ttaaattaat tggatatgat gtattggagg aaaatgtaaa
     4861 atgcatgaaa gaagggttcg tttctttttt gattgctcaa cgacccgaaa aacaggctta
     4921 ttgcgcagta agagacatga gttttcactt gattttcgat aaagaagtta ataaaatcaa
     4981 ttatgtgcct atcgacattt tgatgaagga aaatattgat tattatttga atttcaatga
     5041 ataatcgaat taagcccatg aggattaatc aagagaatca agtagcgttt ttgcagtgca
     5101 aattttcttt taatctacct tgccaagacc aacaaaaata ttgttttgaa aaaaagaagt
     5161 ttggatattt ttgaagaaat aatttccttt aaaaaaatca tttttaagaa gatttcatca
     5221 tgaatacttc tattacttca gattggattc aacctgccct gtatcagcaa ccattggaaa
     5281 acggaatggt aaaatgcgtt ctctgtcccc atcaatgtat ggtgcacgaa ggaaaaaccg
     5341 gactttgtca tacccggcaa aacatcaacg gggttctata tacgcttgct tacgctaatc
     5401 cgtgttcact gaatgtcgat ccaatcgaaa aaaaaccact atttcatttc ctccccggct
     5461 caaaaatttt ttcattggct accgccgggt gcaatttcaa atgcttaaac tgtcagaatt
     5521 gggaaatttc tcaaagctca ccacaagaag tgattcatta cgatttgttg ccggaagcag
     5581 ttgtggaact tgcctcgaat cgacagattg ccagcattgc cttcacttat accgaaccga
     5641 ctgtatttta tgaatatgtt tatgacacgg cgaagttggc acatcaaaaa aacatga
//
LOCUS       DAOSCH010000116         5592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_9154_length_5592_cov_5.944374, whole genome shotgun sequence.
ACCESSION   DAOSCH010000116 DAOSCH010000000
VERSION     DAOSCH010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9154_length_5592_cov_5.944374"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..2055)
                     /locus_tag="PLZ54_07875"
     CDS             complement(217..2055)
                     /locus_tag="PLZ54_07875"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009037509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HOJ66876.1"
                     /translation="MKATITGQAQQIVQTRKEKSGRLQGDNPKIGIRPTIDGRQGGVR
                     ESLEDQTMRMALNLAKFLSSNLKYSDGTPVECVIADTTIGRVDEAAACAEKFKKEGVS
                     ITITVTPCWCYGSETIDMDPHTIKAVWGFNGTERPGAVYLAAALAGYNQKGLPAFGIY
                     GRDVQDAGDTSIPADVQEKLLRFSKAAIAAASMKGKSYLSIGSVSMGIAGSIVDPNFF
                     QEYLGMRNEYVDMSEINRRVQLGIFDQEEFARAMEWTSKYCKVNEGEDKNDEDKKKTR
                     EQLDKEWEFVVKMTIIIRDLMIGNPKLREAGFLEEALGHNAIASGFQGQRQWTDFMPN
                     GDFSEAILNTSFDWNGIREPFVVATENDSLNGVAMLFGKLLTNTAQVFADVRTYWSPE
                     AVERVTGYKLEGIASNGLIHLINSGSAALDGSGQQTKDGKPAMKPFWEITDEEVQKML
                     DATTWYPADCGYFRGGGFSSKFVTKGGMPVTMARVNLVKGLGPVLQLAEGWTVEIPAE
                     VHKILDDRTDKTWPTTWFVPRLTGKGNFKDVYSVMNNWGANHGAFSYGHIGADLITLA
                     SILRIPVCMHNVEEDKIFRPSAWAAFGMDAEGSDYRACATYGAMYK"
     gene            complement(2180..>3177)
                     /locus_tag="PLZ54_07880"
     CDS             complement(2180..>3177)
                     /locus_tag="PLZ54_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ66877.1"
                     /translation="KRIIMKVNFSHSKSKINTLIDAISNDIASGVLKLGDNLPSINET
                     SLKFKISRDTVFKAYKELKRRNLIDSNPMKGYFVKGEVNHVLLLLDTYSPFKQNLYNR
                     FAQNLPKSWKVDLIFHQYNKNLFDTIIRESIGRYSMYVVMNFSNTEFSESLKSIPEGK
                     LLLLDFGDFDKKQYPYLCQNFDQALYDCLSEHKPIISKYKKIVMIFPTENNHPKSSIK
                     FVKEFCSDSNIAFELIEDIYLLNNIEENVLYFIMSHTDLVSIILASKNKNLKIGQDIG
                     ILVYNDEPLLEVIQEGIAAISIDFGLLGEKAAQFIKDKMFVQEFLPTRFIFRPSI"
     assembly_gap    3178..3277
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3278..4386)
                     /gene="fucP"
                     /locus_tag="PLZ54_07885"
     CDS             complement(<3278..4386)
                     /gene="fucP"
                     /locus_tag="PLZ54_07885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HOJ66878.1"
                     /translation="MKSDTKIPLLKGSDGTNYLIPFILVTSLFFFWGVANNMTDTLLS
                     AFKNIMEMSDFQTSFIQMAFYGSYACFAIPAALLIRKYTFKHGIVVGLSLYALGTFLF
                     YPASQLASYGFYLFALYVLAGGCSILETPANPYILTMGRKENMTRRLNFAQSFNPIGS
                     ISGIIISQIFILSQLSQNKSTMNDAELLAVQHKELSAVTLTYMGLGAVLVALLLIIIF
                     YKKMPATKDTTGLSFGATVGRLLKRKHYTLGVVAQFFYVGAQIGVWSYTIRYVMLALG
                     LVDPVTSEIFPAKVQGFIAEHNLWSSIWQLDENSTAENIGNSFYLFSLILFTVSRFIF
                     TALMRYFRPGMLLAVAATGAIISAAIAIFATGMTGV"
     gene            complement(4507..4887)
                     /locus_tag="PLZ54_07890"
     CDS             complement(4507..4887)
                     /locus_tag="PLZ54_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HOJ66879.1"
                     /translation="MNNKSGYPVKKFSQPVKRYCQTLKLKDDATLIEEYKFWHAAENR
                     WPEISEGIKAVGILDMELYIHGNLLFMIVETPLDFDWDKAFGKLANLPRQAEWEAFMA
                     KFQMADPNAGSAEKWTLMERIFSL"
     gene            complement(5202..5330)
                     /locus_tag="PLZ54_07895"
     CDS             complement(5202..5330)
                     /locus_tag="PLZ54_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66880.1"
                     /translation="MKKEEIKLSLILVVGGLGMIFCLFFLQCGLRGDSKLYYSKSS"
     gene            complement(5343..>5592)
                     /locus_tag="PLZ54_07900"
     CDS             complement(5343..>5592)
                     /locus_tag="PLZ54_07900"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ66881.1"
                     /translation="YEKGEPEEASQIKVFSSKGHIVIDNLSAENGEAMIYDMAGRKVG
                     QEKFFGNNITVLNNYPTGVYVVRAFSSKETVTKRIIVQ"
BASE COUNT         1695 a         1109 c          905 g         1783 t
ORIGIN      
        1 atatgaattt tcaaaaacaa catttttaaa aactttcgta cggcagtgat aaaaaatgtg
       61 ggttaagttt tgactaactt aagaataatt atccaactaa tataaaaaag atttgaaggc
      121 aacaataatt aatagtatgc cttcaaatca gatattaatt ttttctcaga aattgattga
      181 ttaattatct gagactccct ttagggttgg ggtcatttac ttatacattg ctccgtaagt
      241 tgcacatgcc cgatagtctg aaccttcggc atccatcccg aatgcagccc atgcgcttgg
      301 tcggaaaatt ttatcttctt ccacattgtg catgcaaaca ggaatacgta atatcgaagc
      361 cagtgtaatt aaatcagcgc cgatatgtcc gtaactgaaa gcgccgtgat ttgcacccca
      421 gttgttcatt accgaataaa catccttgaa atttccttta ccggtcaatc gaggtacaaa
      481 ccaagttgta ggccatgttt tatcggtacg gtcatctaag attttgtgaa cttcggcagg
      541 aatttccacc gtccaacctt cagccaattg aagtacagga ccaagacctt tcacaagatt
      601 tacacgagcc atagttaccg gcattccgcc ttttgttaca aattttgaag aaaatcctcc
      661 gccacggaaa taaccacaat ctgcaggata ccaggttgta gcatctaaca ttttctgaac
      721 ttcttcatcg gttatttccc agaaaggttt catggcaggt tttccatctt tggtttgctg
      781 tccgctgcca tctaaagccg ccgagcctga attaatcagg tgaattaaac cgttcgatgc
      841 gataccttcc agcttatatc cggttacccg ttcaacagct tcaggactcc agtacgtacg
      901 aacatcggca aatacctgtg ctgtattggt aagtaatttt ccaaaaagca tggctacgcc
      961 gttcagtgag tcgttttcag tagctactac aaaaggttcg cgaataccat tccagtcgaa
     1021 tgaagtattc agtatcgctt ctgagaaatc tccattgggc ataaaatctg tccattgacg
     1081 ttgaccctga aaacctgaag caatggcatt gtgtcctaaa gcttcttcga gaaatcctgc
     1141 ttcacgcagt ttcggattgc caatcatcaa atcgcggata ataatggtca tttttacaac
     1201 aaattcccat tctttatcca attgctcgcg tgttttcttt ttatcctcat cgtttttatc
     1261 ttctccttca tttactttgc agtattttga tgtccattcc atggctcttg caaattcttc
     1321 ctgatcgaaa attcccagtt gaacccgacg gttaatttcg ctcatatcca catattcatt
     1381 gcgcatgcca agatattctt gaaagaaatt gggatcgaca attgaacctg caattcccat
     1441 cgaaaccgaa ccgatagaca aataagattt tcctttcatc gaagctgcag caatggcggc
     1501 ttttgaaaaa cgtagtaatt tttcctgaac atctgcagga attgaagtgt caccagcatc
     1561 ctgcacatca cgtccgtaaa taccaaatgc aggtagtcct ttttggttat aaccggctaa
     1621 agcagctgcc agatatacag ctcccggacg ttctgttccg ttgaaacccc agacggcttt
     1681 aatagtgtgc ggatccatat cgatggtttc gctgccataa caccaacagg gcgttacagt
     1741 tatggtaatt gaaacacctt cttttttaaa tttttcggca caagcggcgg cttcgtccac
     1801 tcttccgatt gtagtgtcgg caataacaca ttcaaccgga gtgccatcgc tgtattttaa
     1861 gtttgacgat aaaaatttag caagattcaa tgccattctc atagtctgat cttcgagcga
     1921 ttcacgcact ccgccctgcc gaccatcaat tgttggacga attcctattt taggattatc
     1981 gccctgtaga cgtcctgatt tctcttttct ggtttgcaca atctgttgcg cttgccctgt
     2041 aattgttgct ttcattctga aatgttttat ttaatttgtt tgaatattaa tgtattgtat
     2101 ttgcataaaa ttgtaaattg tacatcgtta aattgaaaat tttactgtcc tattctgtgc
     2161 tgacaattta ttaaaaaaat tatattgatg gtctgaaaat aaatcttgta ggtaaaaatt
     2221 cctgaacgaa cattttgtct tttataaact gagctgcttt ttcgcccagc aatccaaaat
     2281 caatcgaaat agcggcaatt ccttcctgaa ttacctccaa aagaggttca tcgttataca
     2341 ccaaaatacc aatatcctgt cctattttca gatttttatt tttacttgct aaaattatgc
     2401 tcaccaaatc ggtatgcgac attataaagt acaatacatt ttcttctata ttgtttaaaa
     2461 gataaatatc ctcgattaat tcaaatgcta tatttgagtc tgaacaaaat tctttcacaa
     2521 atttgattga acttttagga tgattgtttt cagttggaaa aatcattaca attttcttgt
     2581 actttgaaat tattggcttg tgttcagata aacaatcata taaagcttga tcaaaattct
     2641 gacataaata cggatactgt tttttatcga aatctccaaa atcaagcaat agcaattttc
     2701 cttcgggtat ggatttaagt gattcagaaa attcagtatt cgaaaaattc atcaccacat
     2761 acatgctgta acgtccgatg ctttccctga taattgtatc aaataaattt ttgttgtact
     2821 gatgaaaaat taaatcaact ttccacgatt tgggtaaatt ctgtgcaaaa cgattgtata
     2881 gattttgttt aaaaggtgaa tatgtatcga gcagcaataa aacatgattt acttcaccct
     2941 ttacaaaata acctttcatc gggttcgaat caatcagatt tcttcttttt aattctttat
     3001 acgccttgaa aaccgtgtct cgagaaattt taaactttaa gctcgtttca ttgatcgatg
     3061 gtaaattatc tcccaacttt aacacacccg atgcaatatc atttgatatt gcatcaatca
     3121 gcgtgttgat tttcgattta gagtgagaga aatttacttt cattattatt ctctttannn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaca cctgtcattc cggttgcaaa
     3301 aattgcgatt gcagcagaaa ttattgcacc tgtagcagct actgcgagca acattccggg
     3361 acggaaataa cgcattaaag cagtgaaaat aaatcttgaa acagtgaata aaatcagcga
     3421 gaaaagataa aaggaattgc caatattttc ggcagtagag ttttcatcga gttgccagat
     3481 actgctccat aaattatgtt cagcaataaa tccctgcact tttgccggaa aaatttcaga
     3541 agttacagga tcgacaagcc cgagtgcaag catcacataa cgaatggtgt aagaccaaac
     3601 gccgatttga gctccgacat aaaaaaactg agcaaccaca ccaagtgtat aatgttttct
     3661 ttttagcaat ctgccaactg tagcgccaaa acttaatcct gtagtatctt tggtggcagg
     3721 catttttttg taaaaaatta taattagaag taatgctaca agaactgcac ccaagcccat
     3781 atacgtaagt gtaactgctg ataattcttt gtgctgaacg gcaagcaatt cggcatcgtt
     3841 cattgtgctt ttattctggc ttaactgtga taaaataaat atctgactga tgataattcc
     3901 tgaaattgaa ccaatcggat taaaagattg ggcaaaattt aacctgcggg tcatgttttc
     3961 tttccgaccc atggttaaaa tataaggatt ggcaggagtt tcgaggattg agcaacctcc
     4021 ggctaaaaca tacaatgcaa acaaataaaa gccgtaggat gcaagttgcg aagccggata
     4081 aaacaggaaa gttcctaacg cataaagcga gagccctaca actattccat gtttaaatgt
     4141 atattttctg attagtaaag ctgccggtat cgcaaaacat gcataagagc cataaaatgc
     4201 catttgtatg aacgatgtct gaaaatcgga catctccata atgtttttaa acgcagacag
     4261 aagtgtgtca gtcatattgt tagcaacccc ccaaaaaaag aaaagagaag taactaaaat
     4321 aaaaggaatt aaataatttg taccatcgct gcctttcaat aaaggaattt tagtgtctga
     4381 tttcatgata ttttatttgt ttgtattttt tgatacagac acaatgcatt gcatctgtgc
     4441 atatttcagt tttacatttt tgtctataat ttgtaaatag taaatgatta aatggtaaat
     4501 ggtaattcac aagctaaaaa tccgttccat caaagtccat ttttctgctg aacctgcatt
     4561 cgggtctgcc atctgaaatt ttgccataaa tgcctcccat tctgcttgcc gtggtaagtt
     4621 ggcaagtttt ccgaaagctt tatcccaatc aaaatctaga ggagtctcca caatcataaa
     4681 aagcaaattg ccatgtatat ataattccat atcgaggatc ccaaccgctt ttattccttc
     4741 cgatatttca ggccagcgat tttcagccgc atgccagaat ttatattctt caattaaagt
     4801 tgcatcatcc tttaatttta aagtctgaca ataccgtttt accggctgtg aaaatttttt
     4861 caccggatat cctgatttat tattcataat ttgttcatta ttaagaaaat ataaaattaa
     4921 aaatctggtt gttatttacg atattcaatt ttcacttttt ctctccttct actcttgata
     4981 tgaaacccac atgtattgca tacacaaaca cgaatgaata taatatttag acatgtgggt
     5041 tccatcagac ctttaaaaaa taaattatat tctgatgaaa actaagtaaa gaaaattcat
     5101 ctgtgctgaa ctgtgctgca aaaatagtca aaaatatgag acgatattac tttatcataa
     5161 aaaattagat ttaatcgtta atcgtttgaa acaagtataa attagctact tttgctataa
     5221 tacaattttg aatcccctct caacccacat tgcagaaaga aaaggcagaa aatcattccc
     5281 agccctccaa ccactaatat caacgagagt tttatttctt cctttttcat tgctctccgc
     5341 ttttactgaa caatgattcg ttttgttacc gtttccttgc ttgaaaaagc tcttaccaca
     5401 taaactcccg tggggtaatt gttgagaacg gttatgttat ttccaaagaa tttctcctga
     5461 cccacttttc gtcccgccat atcgtaaatc attgcttctc cgttctctgc actcaagttg
     5521 tcaatcacaa tatgcccttt ggaagaaaat accttgattt gcgatgcctc ttccggttct
     5581 cctttttcgt aa
//
LOCUS       DAOSCH010000117         5529 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_9273_length_5529_cov_5.044392, whole genome shotgun sequence.
ACCESSION   DAOSCH010000117 DAOSCH010000000
VERSION     DAOSCH010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9273_length_5529_cov_5.044392"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..3426
                     /locus_tag="PLZ54_07905"
     CDS             424..3426
                     /locus_tag="PLZ54_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ66882.1"
                     /translation="MKNKFYLLLVLLSFPLLIFAQAQREVKGKILNENGEPVIGANIF
                     ESGNPTNGTISDLEGNFVIKISPQSAIEISYIGYVPQKIKDPKSPLTIQLEVDQRSLD
                     EVVVIGYQTVKKSDLTGAVSVFKPETMKNTVVTGTIGEALYSVPGIFVRSAGAPGNEG
                     FIQIRGTSTFGTSNPLYIIDGIDVGTANRDFNYNDIESIQVLKDASAAAIYGSRAANG
                     VIIITTKKGSKGKMKIDFSAKNTFQWLPQYNLTNREQWITLNDLAFKNAGRQPANHSD
                     GNTNWQDEVFKTGILQDYDLSFSGGGETNTYYLSANYQTNSGTTYGTNSERITARINT
                     SSFRDFGKNVRFTIGENLIISNYFVDELNTNPITDVWRMLPTIPVYDPSNPGGFGYGD
                     GSKDVTFGTNPIARENLENTTNENLRIRGNAFAELSFFKSLKYKFNLGLVTSSDSHMY
                     LRKVGNWTYNQPIDPSSLNRNKALYKFMVYDNTLEFNKTFGVNNIAAVAGTSFSTEDY
                     SQIWGTKNNVLTSGDDYFTQLDAALLDPKTGSYQNLAKLFSIFGRLNYSYDDKYILSA
                     TVRRDASSKFGPNYRNGVFPSIAGAWRISKEKFFNVSWIDDLKIRANYGILGSSNIGY
                     WDWVPFINIFPQAIFGAGQNINTGMTQVKLVNTDLKWEELHQTNAGFDAYLLNRRLEV
                     VGDYFNKTTQDVLTPMQILLTTGNNGGNPMVNAATLKNTGFEVSVNWRDRINDLNYSV
                     GLSTSYLKNEIVELGYGRESFTQWDTKSFVGKPIGDWYLIKTDGIFRSTDEVLNHINS
                     KGQVIQPSAKPGDIRFVDFNDDGQITDADRQYCGHSFPAWNMALNGTLEFKGFDLMFQ
                     FTSSFGKDFKLFNGPRSGYDRFDDNSNYRADYDGYDAVTNPNGVDPRPLYADARNARG
                     DQDRWLENGSYLRLKQVGIGYTFPKKALNNVLSNFRLFVNAQNLFTITKYRGLDPEFL
                     NSNIWDRGYDSAPFPNPYGITVGTQISF"
     gene            3453..5030
                     /locus_tag="PLZ54_07910"
     CDS             3453..5030
                     /locus_tag="PLZ54_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOJ66883.1"
                     /translation="MKKYFYLLIIASTFLLNGCDVDVINPNTLTQATFWKTQRDAEYG
                     VNAIYNMFYKPGTYSRWIWFRLDLTSDEGWSQSPWAELKEWTRFQYVNYNFWEGNAWT
                     YRDCYEAIFRANQALENIPNIEMDETVKNQLLGQAYFLRGLYYYDLALLWGSTNNSLP
                     INLKPSKPADTPEGHTGEAVYNQAISDLTQAVNLLPEQWDSNNLGRATKGAAYALRAK
                     CYMQLHDWTKAKNDLYWLVEGDGKQYYDLVANYEDNFTKDTENNKESVFEIQYSDVHK
                     APAGDGDFDVDPNLGLNRGQFFAPPGIGWTDGELRPWIVEEFKKERDLNGNFDIRLKY
                     TAFYEGMNQDFTDNNRIYRYRLDGADANIWNLQNWKGRVFFRKYGSDYYRDYDDYHNP
                     TNVRLIRYADVLLMYAECIANTENDFSKAVDLVDRVRQRANMPKLSVNHLDATQSKEK
                     FLKRLQTERALELCSEGHRWADIKRWGLVDTQNGLNELIARDADFQNFVIGKHAVLPI
                     PSDDVNNNPNQQQNPNY"
     gene            5032..>5529
                     /locus_tag="PLZ54_07915"
     CDS             5032..>5529
                     /locus_tag="PLZ54_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOJ66884.1"
                     /translation="MKLKYVVILLSLIFLSCRKGKIEDQETNKVLVPLADPYILLYDG
                     MYYAYGTGADNGIEVMTSSDLKTWKRRANTTSGLALYKDDSYGEKWFWAPEVYYVNEK
                     FYMYYSADEHICVAISDSPLGPFRQVEQKPMLEEEKAIDNSLFVDDNGKAYLFFVRFN
                     DGNNIW"
BASE COUNT         1823 a          872 c         1135 g         1699 t
ORIGIN      
        1 caaatcttta accgagaaaa aagttttaaa agaaaatgag tagcaataaa acacagcgag
       61 ttacaaactt gaatatatac tttacacttt acaaacaaag agataacatc atcaggtcaa
      121 attcagctct tttgaagaca agaaaattta tgcattagca aataatttaa gaaagagcaa
      181 tacatcggga atttaggata tgagtattaa attgttgagt agaaatcatt ctttaaaaaa
      241 gagaaaattg cataaccttc gaattttgat acctgtagct ggcaaactaa taagtttaaa
      301 aaatcgaata attgataata tttttttatt ttttgataca aaaattctat gttgaattta
      361 ttttagtatc tacttttatg gagcaaattt ttgtgaaatt tatcaattta aaaaattaaa
      421 tctatgaaaa acaaatttta tttattgcta gttcttttat cctttccgct tttaatattt
      481 gctcaagcgc aaagagaagt caaaggaaaa attttgaacg aaaatggaga acctgtcata
      541 ggggccaata tttttgaatc gggtaatcca acgaatggga ctatttctga tttggaaggc
      601 aattttgtaa taaagatatc tccgcaatcg gctattgaga tatcgtatat tggttatgtg
      661 ccacaaaaaa ttaaagatcc aaaatcacct cttaccattc agttagaagt ggaccaacgt
      721 tctttggatg aagtcgtagt cataggttat cagaccgtga aaaaatcgga tttgaccggt
      781 gctgtttcag tttttaagcc cgaaacaatg aaaaacacgg ttgttaccgg tacaatcggt
      841 gaagctttat acagcgttcc gggtattttt gttcgatcag ccggtgctcc aggaaatgaa
      901 ggttttattc aaatcagggg aacttcaact tttggcacaa gtaatccttt atatattata
      961 gatggaatcg atgttggaac agctaaccga gacttcaact ataatgatat cgaaagcatt
     1021 caagtattga aagatgcttc agcagctgct atttatgggt caagggcagc taatggagtt
     1081 attattatta ccaccaagaa gggtagcaag ggaaagatga agatcgattt ttcggcaaaa
     1141 aacacttttc aatggttacc tcagtataat ttaaccaatc gtgaacagtg gataactttg
     1201 aatgatcttg ctttcaagaa tgccggaagg cagccagcca accatagcga tggtaatacc
     1261 aattggcagg atgaagtttt caaaaccggt attttacagg attatgattt gagtttttcg
     1321 ggtggtgggg aaaccaatac ctattactta tctgccaact accaaaccaa tagtggaact
     1381 acttacggaa ccaacagtga aagaataact gcccgtataa atacttcttc ttttcgcgac
     1441 ttcgggaaga atgtaagatt tacgattggt gaaaatttaa ttatcagcaa ttactttgta
     1501 gatgaattga ataccaaccc gattacagat gtttggagaa tgttgccaac cataccggtt
     1561 tatgatccat ctaatcccgg agggttcggt tatggagatg ggtccaaaga tgttactttt
     1621 ggtactaatc caattgctcg tgaaaatttg gaaaatacta ccaacgaaaa tttaagaatc
     1681 agaggtaacg catttgctga attaagtttc tttaaatcat tgaaatataa gtttaatctt
     1741 ggcttggtta ccagttcaga ttctcacatg tatttacgca aagttggtaa ttggacgtat
     1801 aatcaaccaa tagatccttc tagtttgaac agaaataaag ctttatacaa atttatggtg
     1861 tacgataaca cactggaatt taataagact ttcggcgtaa ataacattgc tgctgtagca
     1921 ggtacaagtt tttccactga agattattct caaatttggg gaacaaaaaa taatgtgcta
     1981 acttcaggag atgactattt cacacaattg gatgctgctt tgttggatcc aaaaacagga
     2041 agttatcaaa atttggcaaa attattctca atttttggac gattaaacta tagttatgat
     2101 gataaataca tcctcagtgc aacggttcgt cgggatgcct cttcaaagtt tggtccaaat
     2161 tacagaaatg gagttttccc atcaatagcc ggagcatgga gaattagtaa ggaaaaattt
     2221 ttcaatgtgt catggattga tgatttaaaa atcagagcaa attatggtat tttgggaagt
     2281 tccaatatag gttattggga ttgggttcca tttatcaata tatttcctca ggcaattttt
     2341 ggtgcaggcc aaaatattaa tacaggtatg actcaggtta aattggtcaa tactgatttg
     2401 aaatgggaag aactacatca aacaaatgca ggattcgatg cttatttgtt gaatcgtcgt
     2461 ttggaagttg tgggtgatta ttttaacaaa accactcagg atgttttgac tcccatgcaa
     2521 atattgttga caacgggtaa taatggaggt aatccaatgg tgaatgccgc cactttaaaa
     2581 aataccggtt ttgaagtttc ggttaattgg agagatcgca taaatgattt aaattattct
     2641 gttggtttga gtacttcata cttaaaaaat gagattgtag aacttggata tggtcgtgaa
     2701 agctttacac aatgggatac caaatctttt gtcgggaaac cgattggtga ttggtatctt
     2761 atcaaaacgg acggaatttt cagatctaca gatgaagtgt tgaaccatat taatagtaag
     2821 ggacaagtga ttcagccgtc tgctaagccc ggagatattc gttttgttga tttcaatgat
     2881 gatggtcaga ttacggatgc cgaccgtcaa tactgcggac attcgttccc agcttggaat
     2941 atggctttaa acggaacgct cgaattcaaa ggttttgatt taatgtttca gtttaccagt
     3001 tctttcggaa aagatttcaa actgttcaat ggaccaagaa gtggttatga caggtttgat
     3061 gacaattcaa attacagagc cgattatgat ggttatgatg cggttaccaa tccaaacggg
     3121 gtggacccac gacctcttta cgccgatgcc agaaatgctc gtggtgatca ggatcggtgg
     3181 ttggaaaatg gcagctatct acgcttgaaa caagtcggaa ttggatacac tttcccaaag
     3241 aaggcgctca ataatgttct tagtaatttt agattgtttg tcaatgctca aaatcttttc
     3301 accattacca aatatcgcgg gttagaccca gaatttctca attccaatat ctgggacaga
     3361 ggttatgata gtgcaccttt cccaaatcct tatggaataa cagttggaac acaaatttct
     3421 ttttaataat tgaccattta aattagaaca caatgaaaaa atatttttat ttattgataa
     3481 tagcatcaac tttcctttta aatggatgtg atgtagatgt tataaatccc aatacactta
     3541 ctcaggcgac attttggaaa acgcaacgtg atgcggaata tggtgtcaat gcaatttata
     3601 acatgtttta taaaccaggc acttacagcc gttggatatg gttccgactg gatttgactt
     3661 ccgacgaagg atggagtcaa agtccctggg cagagcttaa agaatggaca cgttttcaat
     3721 atgtaaatta caatttctgg gaggggaatg catggactta cagagattgc tacgaagcaa
     3781 ttttcagagc taatcaggct ttggaaaata tcccgaatat tgaaatggat gaaacagtta
     3841 aaaatcagct tttaggacag gcttattttt taagaggttt gtattattat gatttggctt
     3901 tactttgggg aagtaccaac aatagtcttc cgattaactt aaaaccatcc aaacctgctg
     3961 atactcctga gggacataca ggagaagctg tttacaatca ggcaatcagc gatttaacac
     4021 aggcggtaaa tttattgccg gaacaatggg acagcaataa tttgggacgt gctacaaaag
     4081 gagctgctta tgccttaaga gcaaaatgtt atatgcaatt gcatgattgg acaaaagcaa
     4141 aaaatgattt atactggttg gttgaaggag acggcaaaca atattatgat ttggtagcta
     4201 attatgaaga caactttaca aaggataccg aaaacaataa agaatccgtg tttgaaatac
     4261 aatactccga cgttcacaaa gcccctgccg gtgatggtga ttttgatgtc gatcctaatt
     4321 taggattgaa tcgtggacag ttttttgctc ctcccggaat tggatggaca gacggagaat
     4381 tacggccatg gatagtggaa gaatttaaga aagaaagaga tttgaatggg aacttcgaca
     4441 ttcgtttaaa atacacagct ttctatgaag gtatgaatca agattttacc gataataaca
     4501 gaatttatcg ttataggctt gatggtgctg atgctaatat ttggaattta caaaactgga
     4561 aaggacgagt attttttaga aaatatggtt ctgattatta tcgcgattat gatgattatc
     4621 acaatccgac caatgtaagg ttgattcgtt atgccgatgt attgcttatg tatgccgaat
     4681 gtatagccaa tactgaaaat gatttttcaa aagctgttga cttggttgac agggttcgtc
     4741 aaagagcaaa tatgcctaaa ctgtctgtta atcatcttga cgcgacacaa tcaaaagaaa
     4801 aatttttgaa aagattacaa accgagcgtg ctttagaatt gtgttcagaa ggtcatcgtt
     4861 gggcagatat aaaacgttgg ggattggtag atactcagaa tggtttaaac gagctgattg
     4921 cgcgtgatgc agatttccaa aattttgtta ttggaaaaca tgctgtgttg ccaattcctt
     4981 cggatgatgt gaacaacaat cctaatcaac aacaaaatcc caattattaa aatgaagtta
     5041 aagtatgttg ttatattgtt gagtttgatt tttctgtcgt gcagaaaagg aaaaattgaa
     5101 gatcaggaaa caaataaggt cctggttcct ctggccgatc cgtatatttt gctttacgat
     5161 ggaatgtatt atgcatatgg taccggcgca gacaatggta ttgaagtgat gacttcatcc
     5221 gacctcaaga catggaaacg gagagcaaat acaacaagtg gacttgcact ttacaaggat
     5281 gattcctacg gcgagaaatg gttttgggca ccggaagtgt attatgtgaa tgaaaaattt
     5341 tacatgtact attcggctga tgaacatatc tgcgttgcca tctccgattc tcctttgggc
     5401 ccatttcgac aagtcgaaca gaaacccatg cttgaagagg agaaagccat tgacaattcc
     5461 ttatttgtgg atgataatgg aaaagcctac ttgttttttg ttcgcttcaa tgatggaaac
     5521 aatatttgg
//
LOCUS       DAOSCH010000118         5517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_9305_length_5517_cov_5.564995, whole genome shotgun sequence.
ACCESSION   DAOSCH010000118 DAOSCH010000000
VERSION     DAOSCH010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9305_length_5517_cov_5.564995"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            281..4618
                     /locus_tag="PLZ54_07920"
     CDS             281..4618
                     /locus_tag="PLZ54_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HOJ66885.1"
                     /translation="MAKILGLDLGTNSIGWAIRETDIFEREHYYELFQSDKYERFKEN
                     PDFKKSNLDNEIVDFGVCHFEKGVGDGKSGEFSLAAERRKNRSKRRLYNAKRYRKWEL
                     LKCLIDHDMCPLTSDELKLWSVGNWIEIDGKWKNKGRIYPINNVDFQRWLAFDPAIFG
                     DKGKSDKGNPIRKNPYDLRCELINRKEENEYLRKLKTGRALYHLAQRRGFKSSRKSGQ
                     STYAKNEDIEKLKLENPDFQIALLAKEKLNEGKRFRASGVIQRKYFEDEFYAICEKQE
                     IDQDLVEILHKAIYFVRPLRSQKGLVGNCTLEKGKPRIPISHPKFEEFRALQFINNIK
                     WREKGSKEFVEIPISLKKKILEELFFRKLSSGKNKGKISEDNYFKFEEIIKKYSENGT
                     YEFNYKNLPNVSTCPTIASIMNVFQDEWSDCFIQDENRYGINWDGLSIKYKTQDYKNL
                     GRRLKRKIVKGKIKYIEAELKEEKTLGIDEIWHLLFDYIQTKDNQDALEIFCKEVLGF
                     DENKAKSFAAIDIPQGYGSLSYKAISKILPFLQEGYIYSEAVLFANLKSVLGDKFEDN
                     KEDAKRIIAETIKEVSQKKEILNIVNGLIQNYFSKAEISHAKGVDKCIKDLAWQETNN
                     RLKQYFGVADWNKKSETEKEKLEQKVFELYLRFLNGEQKDDEKASAKGIKVPEIDYYK
                     LPRLDIAIKEKLKAKFNVSDNALKLLYHPSDIEMYPVSKTEIKGTDIKLLESPQPPSK
                     GWKNPMAMRTLHELRHLVNYLLKIGKIDKDTKVVVEMARELNDANKRWAIETYQRYRE
                     EENKEFAKAIVGVVKQKYPNINENDIENINRIRLWWEQIDNNEETYRQIKSLKEDVEK
                     YRLWKEQNCVCMYTGKLINITNLFDGTQTQLMHTFPISISFDSSLANLTVGDAFYNNE
                     IQGNKIPTQLPNYKNDITINGRTYTAIEPRLKKWQDKIQHLRDLIDDNKRRTKKTQDV
                     ETKNDLIRKRHLLQFDYEYWQKKVDTFTLEEVPLKWKNSQLVDTQIISKYARAYLKTV
                     FEKVDVQKGTITDSFKKIYQIKGEEQKDRSKHSHHAQDAAVLTLIPVSAKRDAILEEY
                     YKSLEKNEKYHIKPYESYVSAHIIEIENNVIINHIPHDKTLTPTIKHIRKRGKKTGKI
                     AQGDSIRGQLHKETFLGAIKVLERNSQGFPVKENGEYKVLKDKTGKDEIWIVSRKSIK
                     DINIDKDIIVDELLKQHIHKQLNNGVELSNVVDFNNKPIRHIRVRYKAGKGFLSKDKT
                     LPLKSHTYNSKRNHKINYLVQNEENYLFLFYEGINEKNQIIRHYRILNLFDIAQLKIR
                     SIHDIRKEPKFKTILKGKISLNLKNILKVGDRVILFKESKDEITEENLKSRLYTIFKF
                     NEPAKNTAYIYLQHQNEARPDNELDNGEKVFNPFKYQPRLELTPDKMNCLFEGIDFEI
                     KPDGTIIFK"
     gene            4620..>5517
                     /gene="cas1"
                     /locus_tag="PLZ54_07925"
     CDS             4620..>5517
                     /gene="cas1"
                     /locus_tag="PLZ54_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOJ66886.1"
                     /translation="MIKRTLYFGNPAYLSLRKEQLVVQLPEVVKNEDLPEKIKQESIV
                     TIPIEDIGVVILDHKQITITQGLMEKLLENNCALITCDSTRMPVGLMLPLAGNTTQTE
                     RFSDQINASVPLKKQLWQQTVQAKIENQAYVLEKMAGGIVKNMNAWAADVKSGDPDNY
                     EARAAVYYWANLFPGIQGFSRNRDGVPPNNLLNYGYAVLRAVVTRSLVASGLLPTLGI
                     HHRNKYNAYCLADDVMEPYRPFVDRLVFEIVLENENIDEITQPIKTRLLGIPVLDVMM
                     NGQRSPLMVAVGLTTASLHQCFA"
BASE COUNT         2116 a          807 c         1062 g         1532 t
ORIGIN      
        1 tcaagggaat tgaattgttt ttctgataca aaaatgcccc gcagataggc gaggcactaa
       61 atgttttcac aacggaataa tccttattgt gatttgcttt caatgtatta tgagagaatt
      121 ttttagaatg ttattgtttt ttaaaatatt taatcttaat tttaaggcca aaaataatcc
      181 cacggagttc gcgggattaa aggattacaa atccttcggc ttatagcctt gttgtgattt
      241 gcaagacaaa gataatgaat atttttaata aaaaacaaat atggcaaaaa ttttaggact
      301 tgacttaggt acaaattcaa tcggttgggc aatacgcgaa accgatatat ttgaaagaga
      361 acattattat gaattgtttc aaagtgataa atacgaaaga tttaaggaaa atcctgattt
      421 taaaaagtca aatctcgata acgaaattgt agatttcggc gtttgccatt tcgaaaaagg
      481 agttggcgac ggaaaatcgg gggaattctc attagctgct gaaagaagga aaaacagaag
      541 caaacgccga ttgtataatg caaaaagata taggaaatgg gaattgttga aatgtctcat
      601 tgaccatgat atgtgtcctt taacttcaga tgaactaaaa ttatggagtg taggtaattg
      661 gattgaaatt gatggaaaat ggaaaaacaa gggacgaatt tatccgataa ataatgtaga
      721 ttttcaacgt tggcttgcat tcgatcctgc aatttttgga gataaaggaa aatcggacaa
      781 agggaatcct ataagaaaga atccttatga tttacgatgt gaattgatta accgcaaaga
      841 agagaatgaa tacttaagaa agttgaaaac aggaagagca ttatatcatt tagcgcagag
      901 acgtggtttc aaatcgagca gaaagagtgg gcaatcaacc tatgccaaaa atgaagatat
      961 tgagaaactt aagttggaaa atcctgattt tcaaattgct ctattagcaa aagaaaaatt
     1021 aaacgaaggt aaacgttttc gggcaagcgg tgtaattcaa cggaaatatt ttgaagatga
     1081 attttatgcc atttgtgaaa agcaggaaat tgatcaagat ttagtagaaa ttcttcataa
     1141 agcaatttat tttgttcgtc cattacgctc acaaaaagga ttggttggca attgtacttt
     1201 ggaaaagggc aaaccccgta ttccgattag tcaccccaag tttgaggaat ttagagcatt
     1261 gcaatttata aacaatataa aatggagaga aaaaggctca aaagaatttg ttgaaattcc
     1321 tatctctttg aaaaagaaaa ttttggaaga attatttttt aggaagctat catctggaaa
     1381 aaacaaaggg aaaattagtg aagataatta tttcaagttt gaagaaatta tcaaaaaata
     1441 ttcggaaaat ggaacctatg aatttaacta taagaatctt ccaaatgttt ccacttgtcc
     1501 aaccattgct tcgattatga atgtatttca agatgaatgg agcgattgtt ttatacaaga
     1561 tgaaaataga tatggtatta attgggacgg tttatcaata aagtataaaa ctcaagatta
     1621 taaaaattta ggcaggcgac tgaaaagaaa aatcgtaaaa ggtaaaataa aatatattga
     1681 agcagaatta aaagaagaaa aaacattagg aattgacgaa atttggcact tgcttttcga
     1741 ttatatacaa acgaaagata atcaagatgc tttagaaata ttttgtaaag aggtcttagg
     1801 ctttgatgaa aacaaagcga aaagtttcgc agctattgat atccctcaag gatatggttc
     1861 attgagttat aaagcaattt cgaagattct cccttttttg caagaaggtt acatatattc
     1921 cgaagctgta ttgtttgcca accttaaaag tgtattaggc gataagtttg aagacaataa
     1981 ggaagatgca aaaagaataa ttgcagaaac cattaaagag gtttcacaaa aaaaagagat
     2041 attgaatatt gtcaatggat tgattcaaaa ctatttttct aaagcagaaa taagtcatgc
     2101 aaaaggtgtt gacaaatgca ttaaagacct tgcatggcaa gaaacaaata acagactgaa
     2161 gcaatatttt ggcgtcgcag attggaataa aaagtcagaa acggaaaaag aaaagttgga
     2221 acaaaaagtt tttgaattgt acttaagatt tttaaatgga gaacaaaaag atgatgaaaa
     2281 ggcctctgct aaaggaatca aagtccctga aattgattat tacaaactac cccgcctcga
     2341 tatagcaata aaagaaaaat taaaagccaa atttaatgta tcggataatg cattaaagct
     2401 cttatatcat ccgtcggata ttgaaatgta tcctgtttcg aaaaccgaaa tcaaaggtac
     2461 cgatattaaa ttgcttgaaa gccctcaacc tccttccaaa ggctggaaaa atccgatggc
     2521 gatgagaact ttgcacgaat tacgtcactt agtgaattat ctgttgaaaa ttgggaaaat
     2581 agataaagat acaaaagttg tggtcgagat ggcaagggag cttaacgatg ctaacaaacg
     2641 ttgggctatt gaaacatatc agaggtatag agaagaagaa aataaagaat ttgcaaaagc
     2701 tattgttggg gtggttaaac aaaaatatcc caacattaac gaaaatgata tagaaaatat
     2761 taatcgaatt cgcttgtggt gggagcaaat tgataataat gaagaaacct acaggcaaat
     2821 aaaatcactt aaagaagatg tggaaaaata ccgactttgg aaagaacaaa actgtgtttg
     2881 tatgtatacc ggaaagttaa tcaatattac taatcttttt gacgggacac aaacacaact
     2941 aatgcacact ttccctataa gtatttcatt tgatagttca ttagctaatc ttactgttgg
     3001 agatgccttt tataacaatg aaattcaagg caataaaata ccaactcaat tacctaatta
     3061 taaaaatgat attaccataa atggaagaac ttatactgct atcgaaccaa ggctgaaaaa
     3121 gtggcaagac aaaattcaac atttgagaga tctgattgat gataataaaa ggcgaactaa
     3181 aaaaacgcaa gatgtcgaga caaaaaatga cttgattaga aaacgacatt tattacaatt
     3241 tgattatgaa tattggcaga aaaaagttga tacttttact ctggaagagg tgccattgaa
     3301 atggaagaac agccaattgg ttgatacgca aataatctca aaatatgcac gggcgtatct
     3361 aaaaactgtt tttgagaaag ttgatgtaca aaaaggtact ataactgaca gtttcaagaa
     3421 aatatatcaa ataaaaggcg aagaacaaaa agatcggagt aaacatagcc atcatgcaca
     3481 agatgccgct gttttgacct taattcctgt ttctgctaaa cgagatgcta ttttggagga
     3541 atattataaa tcgttagaaa aaaatgagaa atatcatatt aagccctatg aatcgtatgt
     3601 ctcagcacat atcattgaaa ttgagaacaa tgttataatc aatcatattc ctcacgataa
     3661 aacgcttaca cccacaataa agcatataag aaagagaggc aagaaaacgg ggaaaattgc
     3721 tcaaggagac agcataagag gacaattaca taaagagact ttcttgggag ctatcaaagt
     3781 attagaaagg aatagtcaag gttttccggt caaagaaaat ggagaatata aagttcttaa
     3841 agataaaact ggcaaagatg aaatttggat agtttctaga aaatcaatta aagacataaa
     3901 tattgataag gatatcattg tcgatgaatt attgaaacaa catattcata agcaattaaa
     3961 taatggcgtt gaattgtcaa atgtagtaga ttttaataat aagccaatac gtcatatcag
     4021 agttcgatat aaggctggca aaggattttt gtctaaggac aaaaccctgc cacttaaatc
     4081 acacacatac aattctaaaa gaaatcacaa aatcaattat cttgttcaaa acgaagaaaa
     4141 ttatttgttt ttattttatg aaggtattaa tgaaaagaat caaattattc gtcattacag
     4201 aattcttaat ttgtttgaca ttgcacaatt gaaaattcga agcattcatg atatacgaaa
     4261 ggaacctaaa tttaagacta ttttaaaagg aaaaataagc cttaatttaa agaacatttt
     4321 aaaagttgga gatagagtaa tattgtttaa agaaagtaag gatgaaataa cagaagaaaa
     4381 tttaaagtca agactatata cgatatttaa attcaatgaa cctgctaaaa atactgctta
     4441 tatttatttg caacatcaga atgaagcaag gccagataat gaattagata atggagaaaa
     4501 agtatttaac ccttttaaat atcagccaag acttgaattg actccagaca aaatgaattg
     4561 tctttttgaa ggaattgatt tcgaaataaa accggatgga acaataattt tcaagtaaaa
     4621 tgatcaagcg aacgctctat tttggtaatc cggcttatct ttcgttgaga aaggaacaat
     4681 tggtggttca attgcccgaa gtggtcaaaa atgaagattt gcccgaaaaa atcaaacagg
     4741 aatcgattgt taccattccc atcgaagaca ttggcgtagt gattctcgac cacaagcaga
     4801 ttacgattac gcaaggcttg atggagaaac tcttggagaa caattgtgcc cttatcactt
     4861 gcgacagcac tcgaatgcca gtcgggctta tgttaccttt ggcaggtaat acgactcaaa
     4921 cggaacgttt cagcgatcaa atcaatgctt ccgttccact aaaaaaacag ctttggcagc
     4981 aaactgtgca agccaaaatc gaaaatcagg cctacgtgct tgaaaaaatg gcgggaggaa
     5041 tcgttaaaaa catgaacgct tgggcggctg acgtcaaaag cggcgatccc gacaattacg
     5101 aagcccgagc agcagtgtat tattgggcaa atcttttccc tggcattcaa ggattttcca
     5161 gaaatcggga cggcgtccct cccaataatt tgttgaatta cggttatgcc gttttgcgtg
     5221 ctgtggtaac tcgttcactt gtagcaagtg gattgcttcc gacactgggc attcatcatc
     5281 gcaacaaata caatgcctat tgcttggctg atgatgtgat ggagccttac cgaccctttg
     5341 tggaccgatt ggtttttgaa atcgtgctcg aaaacgaaaa tattgacgaa ataacccaac
     5401 ctattaaaac ccgattattg gggattccgg ttctcgacgt catgatgaac ggacaacgca
     5461 gcccgctgat ggtggcggta ggactaacca cagcatcgct tcatcaatgt ttcgcgg
//
LOCUS       DAOSCH010000119         5509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_9325_length_5509_cov_7.068023, whole genome shotgun sequence.
ACCESSION   DAOSCH010000119 DAOSCH010000000
VERSION     DAOSCH010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5509
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9325_length_5509_cov_7.068023"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1768)
                     /locus_tag="PLZ54_07930"
     CDS             complement(<1..1768)
                     /locus_tag="PLZ54_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ66887.1"
                     /translation="MLRIKLLLLSSLFSVITFAQWQPVGEKIKTRWSSQIDVNNVLPE
                     YPRPLMERSEWMILNGLWNYAILPVGKPAPSAFDGKILVPFAVESSLSGVQKNVGKAN
                     ELWYEREFTIPSKWKNNRVLLHFGAVDWKTDVWVNDVKVGRHTGGYTPFSFEITTALK
                     NGSNKLIVKVWDPTNDGYQPRGKQVNKPEGIWYTPVTGIWQTVWLEPVPDKFITQVKT
                     TPNIDINILNVEVATNNFSASDRIEVKVMDGSKVVATAQSINHLPVEIVMPDQVKLWS
                     PDSPFLYDLEITLWNGTKQIDKVKSYAAMRKYSIKRDENGIVRLQLNNKNLFQFGPLD
                     QGWWPDGLYTAPTDEALKFDIQKTKDFGFNMIRKHVKVEPARWYTYCDRLGMIVWQDM
                     PNGDGGPQWQPHQYFSGLEWLRSPESEANFRKEWKEIIDFLYSNPCIGVWVPFNEAWG
                     QFKTKEIVEWTKEYDPSRLVNPASGGNHYPVGDMLDLHNYPNPALYLYDAQRATVLGE
                     YGGIGWAVKGHLWEPDRNWGYVQFNSAKEVTDEYVKYAEQLMNLILHGFSAAVYTQTT
                     DVEVEVNGLMTYDREIIKMDESR"
     gene            complement(1876..>5509)
                     /locus_tag="PLZ54_07935"
     CDS             complement(1876..>5509)
                     /locus_tag="PLZ54_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOJ66888.1"
                     /translation="ANDSVRGVVSDMIIDSDGDIWISVEEKGVYLYRYESNKLDHFPV
                     KTPPAGLKGIKLCENKGKGVWAFPYSMPFLQIDKKNRKIQPFSLNQGNEILYNTGEIG
                     SVIEEQNNLLVGTSLQGLLQINLVERTYNKLLASDETGQPIFVRNILRKGDDLWVATE
                     SGIYIYNLYSREVINLRHNNVIPYSLSDNAVYSLYEDNEGGVWMGTYFGGVNYFPNRL
                     TYFEIFYPLENENSIKGRRVREFCENTDGKLWIGTEDNGLNLFDPETSKFLPVDSKLN
                     SLYHNIHALFRDGNTLWIGTFSKGLNRYDLKTKTLKIYYKTSQPNSLSENSVFSICKD
                     RQNTLWIGTLSGLNIFNYQNDSFTRIKELEGAFVQDIFEDTDGNIWVATFVKGLFLFN
                     PKTHTWKNFVHTSNAGSLPYNKTTSVFEDSKRRIWITTEGGGFCRYDKKNQSFVTVNS
                     TNGLPNDVVYQIQEDNSGNLWLSTNSGLVKYVPETGIMENFTVNDGLKTNQFNYKSSY
                     KAPDGTLYFGSIDGFVRFNPAYFKENNLLPPIVLTDFYLNNSPANVSDADSPLKENIS
                     FAEKIRLPYNKNSFTIKYAILNYSGLRPKNVVYKLEGFDRQWIPANGSQSVVYSNLNP
                     GKYRLMISFDTNSSGGMNKVSKTLDIVIKPPFWETWWAYLLYFLITIAGIFELINYFR
                     QRTKRIHLEKMRDFEQEKEKELYESKIDFFTNVAHEIRTPLSLIKAPLDQILRNDETV
                     TPDVKDNLKVMSKNTDRLLDLTNQLLDFRKTEAELYKLKEKRTNLSELMKETFDRFTP
                     FAKQKNISFHLNLPENEVFTKIDAEAFTKIISNLINNAVKYCDSMVNISLEVVAENEI
                     EKLVFKTENDGAIVPERFKNEIFKPFTRLNKEKDKIVTGTGIGLALTKSLTELLKGDI
                     TYKVENGLNVFRVTLPFNPLTGNEWIEEKTEMQETPSRKEEDKVEKSLKRPLILLVED
                     DKGMMEFLTKCLKREYSVLSASDGKEALEVLKNKNIDLVVSDVMMPEMDGFELTSYIK
                     SHIEYNHIPVILLTAKVNVQSKVQGFEVGADAYIEKPFSVDVLLAQIASLLQNREKFR
                     EAFLKNPFKGLNHVELNKSDEEFLQKLDDIIQENISNPDFNIEDLSEHFFMSRASFYR
                     KIKGILDLTPNEYLRVARLKKAAQLLKEGNQRITEICYLVGFNSPSYFAKCFQQQFGV
                     LPKDFS"
BASE COUNT         1647 a         1158 c          942 g         1762 t
ORIGIN      
        1 ctctcgattc atccattttt attatttcac gatcgtaagt catcaaacca ttcacctcca
       61 cttcaacatc agtagtctgg gtataaacgg ctgcggaaaa accatgcaga atcaaattca
      121 tcagttgttc ggcatatttc acatattcat cggtaacttc tttggcactg ttaaactgaa
      181 cgtatcccca gttgcgatca ggttcccata aatgtccctt cacagcccag ccaattccgc
      241 catattctcc caaaactgta gcacgctgtg catcatacag atacaatgca ggattaggat
      301 aattgtgaag atccagcata tcgccaaccg gataatgatt gccaccactg gcaggattta
      361 ccaatcgtga aggatcatac tctttggtcc attctacaat ttctttggtt ttaaattgtc
      421 cccatgcttc attgaaaggc acccaaacgc ctatacacgg attggaatac agaaaatcaa
      481 ttatttcttt ccattccttg cggaaattcg cttcggattc gggcgaacga agccattcca
      541 aaccgctgaa atactgatga ggttgccatt gcgggcctcc atctccattt ggcatatctt
      601 gccaaacaat catgcccaga cggtcgcaat aagtgtacca gcgcgcaggt tcaactttta
      661 catgtttgcg aatcatgtta aaaccaaaat ctttggtttt ctgaatatca aattttaaag
      721 cttcatcagt tggagcagta tacaaaccat caggccacca accctgatcc agcggaccaa
      781 attggaacaa attcttgttg ttcagctgta aacgaacaat tccattctca tctcgcttaa
      841 tggaatattt tcgcatagca gcataacttt taactttgtc gatttgtttt gttccattcc
      901 aaagcgtgat ttccaaatcg tacaaaaaag gactgtcggg cgaccaaagt ttaacttgat
      961 caggcataac aatttctacg ggaagatgat tgatggactg agccgtagcc acaacttttg
     1021 agccatccat aacttttact tcaattctgt ccgaagcaga aaaattgttt gttgcaactt
     1081 caacattcaa aatatttata tcaatattcg gtgttgtttt tacctgagta ataaatttat
     1141 ccggaacggg ttcgagccaa actgtttgcc aaatgccggt tactggcgtg taccaaatgc
     1201 cttcgggttt attcacttgc ttcccacgtg gctgataacc atcattggtt ggatcccaaa
     1261 ccttaactat caatttgttt gaaccgtttt ttagtgcagt agtaatttca aatgaaaaag
     1321 gagtatatcc tccagtatgc cgaccaacct taacatcgtt tacccaaacg tcggttttcc
     1381 agtccaccgc tccgaagtgg agaagtacac gattattttt ccattttgaa ggtatggtga
     1441 attcacgttc gtaccataat tcgtttgcct ttcctacatt tttctgaaca cccgacaaac
     1501 ttgattcaac agcaaatgga accagaattt taccatcaaa agcggaagga gctggtttcc
     1561 caaccggaag aatggcataa ttccataaac cattgagaat catccattcc gaacgttcca
     1621 tcaaaggccg aggatattcg ggcaatacat tattgacatc aatctgggac gaccagcgtg
     1681 ttttaatttt ttctccgact ggctgccatt gggcaaaagt aatgacacta aacagtgaac
     1741 ttaacaataa taattttatt ctcagcattg gctaaatttt tagtattctt tgtcaagcaa
     1801 acttagctta aaattcaata ttcaaaagaa aaaaatgaat cgaaaaataa aaatatcatc
     1861 tcaaaatagc agaatttacg aaaaatcttt gggcaaaacg ccaaattgtt gctgaaaaca
     1921 tttcgcaaaa taagaaggag aattaaatcc aaccaagtaa caaatttccg ttattcgttg
     1981 gttgccttcc ttcaagagtt gcgctgcttt tttaagcctt gccacccgaa gatattcatt
     2041 gggagtcaaa tcgaggattc cttttatttt cctgtaaaag ctggcacggc tcataaagaa
     2101 atgttccgac aaatcttcaa tgttaaaatc aggattcgat atgttttcct gaataatgtc
     2161 gtccagtttt tgcaaaaatt cttcatcgga tttattcaac tctacgtgat tgagtccctt
     2221 aaaaggattt ttcaagaaag cttcgcgaaa tttctcccta ttttgcaaca aacttgctat
     2281 ttgagccagt aaaacgtcca ccgaaaaagg tttttcgata taggcatcgg ctcccacttc
     2341 aaaaccttgc actttggatt gaacgtttac tttggccgtt aaaagaatta ccggaatatg
     2401 attgtattcg atatgcgatt tgatatagga tgtcaattca aaaccatcca tctcgggcat
     2461 cattacatca ctgacaacaa ggtcgatatt tttatttttc aaaacctcca aagcttcttt
     2521 accatccgaa gcagaaagaa cactgtattc cctcttcaaa cattttgtca aaaattccat
     2581 catcccttta tcatcttcaa ccaaaagaat taacggccgt tttaacgatt tttcaacttt
     2641 atcttcctct tttctactgg gcgtttcctg catttcggtc ttttcttcaa tccactcgtt
     2701 gccggttaaa ggattaaatg gtagagtaac ccgaaacacg ttcaaaccat tttcaacttt
     2761 gtaagtaatg tcgcccttta aaagttccgt taacgacttg gtcaaagcca aaccaatgcc
     2821 cgttccggta actattttat ctttttcctt attcaatcgt gtaaagggct taaatatttc
     2881 atttttaaaa cgttccggaa caatagctcc atcgttttct gttttaaata cgagtttttc
     2941 aatttcattt tcagccacta cttcgagcga aatgttcacc atactgtcgc aatatttcac
     3001 ggcattgttg atcagattgc taataatttt tgtgaacgct tcagcatcaa tttttgtaaa
     3061 aacttcattt tcaggaaggt ttaaatggaa tgaaatattt ttttgttttg caaacggggt
     3121 gaaacggtca aaggtttctt tcatcaattc cgacaaattg gtgcgtttct ctttcaattt
     3181 atacagttcg gcttccgttt tcctgaaatc gagtaactga ttggttaaat ccagtaaccg
     3241 atcggtgttt ttactcatta ctttcaaatt gtccttcaca tcgggagtaa cggtttcatc
     3301 gtttcgcaaa atttgatcca aaggagcttt aattaacgac aatggagtcc tgatttcatg
     3361 cgctacattg gtgaaaaaat ctattttcga ttcatagagt tctttttcct tttcctgctc
     3421 gaaatctcgc attttttcca gatgaattct ctttgtccgc tgacgaaaat aattgatcaa
     3481 ctcaaaaatt ccggcaatag tgatgagaaa atacagcaaa taggcccacc atgtttccca
     3541 aaagggtggt tttataacaa tatccaatgt tttactgact ttgttcatgc cacctgagga
     3601 atttgtatcg aaagagatca tcaaccgata ttttccggga ttcaaattgg aataaacaac
     3661 cgattgagaa ccattggccg gaatccattg cctgtcgaat ccttccagct tgtaaacaac
     3721 atttttaggg cgtaaaccgg aatagttcaa aatggcatat ttaatggtaa aagaattttt
     3781 attgtaagga agtcgaattt tttctgcaaa cgaaatgttt tctttcaaag gtgaatctgc
     3841 atccgaaaca ttggcgggcg aattatttaa atagaaatcg gttaaaacaa tgggaggcaa
     3901 gaggttgttt tccttaaaat aagcaggatt gaaacgaaca aaaccatcaa tcgaaccaaa
     3961 ataaagcgtt ccgtccggag ctttgtaact cgatttgtaa ttgaactgat tggttttaag
     4021 tccgtcgttg actgtgaaat tttccattat tcccgtttca ggaacatatt ttaccaaacc
     4081 ggaattggtg gaaagccaca aattgcccga attgtcttcc tgtatttgat aaaccacatc
     4141 attgggtaaa ccgttggtgc tgttgacagt tacgaaagat tgattttttt tgtcgtacct
     4201 gcaaaaacca ccgccttcag ttgttatcca aattctcctt ttgctgtctt caaatacaga
     4261 agtcgttttg ttgtaaggaa gactgccggc attcgaagtg tgaacgaagt ttttccatgt
     4321 gtgagttttg ggattgaaca gaaacaaacc ttttacaaaa gtagcaaccc agatatttcc
     4381 atccgtatct tcaaaaatat cctgaacaaa agctccttcc aattctttaa ttcgggtaaa
     4441 tgaatcgttt tggtaattga aaatattcaa tcccgataaa gttcctatcc ataacgtgtt
     4501 ctgtcgatct ttgcaaatgg agaaaacgct gttttccgac aaagaatttg gctgactcgt
     4561 tttataatag attttgagtg ttttcgtttt caaatcatac cgattcaaac ctttggaaaa
     4621 agttccgatc caaagggtgt ttccatctct gaaaagtgca tgaatattgt ggtataagga
     4681 gtttaattta ctgtcgacgg gaaggaattt tgaagtttct ggatcgaaca aattcaatcc
     4741 gttgtcctcc gttccaatcc acaattttcc atctgtattt tcacaaaact ctctaaccct
     4801 tcttcccttt attgaatttt cattttcaag aggataaaaa atttcgaaat aagtcagtcg
     4861 gttcggaaaa taatttactc cgccaaaata agttcccatc catacgccgc cttcattatc
     4921 ttcataaagg gaataaacag cattgtccga aagcgaatag ggaataacat tattgtgcct
     4981 taaattaatg acttcacggg aataaagatt gtaaatataa attcccgatt ccgttgccac
     5041 ccacaaatca tcaccttttc gtaagatatt tcgcacaaaa atcggctgtc cggtttcgtc
     5101 cgaagccaat aatttgttgt aagttcgttc taccagatta atttgtaaaa gtccctgcaa
     5161 ggaagtacct accagcaaat tgttttgttc ttcaattacg cttccgattt cacccgtatt
     5221 ataaagaatt tcattgccct gattcaaaga aaacggttgg atcttacggt tttttttgtc
     5281 aatttgcaga aacggcatgg aataaggaaa agcccaaact ccttttcctt tgttttcgca
     5341 gagtttaatt cctttcaatc ctgcgggagg cgttttaacc gggaaatgat ccaatttgtt
     5401 gctctcatat cgatacaaat aaactccctt ttcttcgacc gaaatccaga tatctccatc
     5461 actgtcgata atcatgtcgc tgacgacacc tcttactgaa tcatttgcg
//
LOCUS       DAOSCH010000120         5401 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_9580_length_5401_cov_5.561915, whole genome shotgun sequence.
ACCESSION   DAOSCH010000120 DAOSCH010000000
VERSION     DAOSCH010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9580_length_5401_cov_5.561915"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLZ54_07940"
     CDS             <1..192
                     /locus_tag="PLZ54_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66889.1"
                     /translation="LLHTQTRMNIIFRHVGSIKPLKNKLYSDEIRRNLCTHSRYYEVV
                     FLREKNFIRIRYFLLQARA"
     gene            216..539
                     /locus_tag="PLZ54_07945"
     CDS             216..539
                     /locus_tag="PLZ54_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66890.1"
                     /translation="MFFNILTYDGTDYRFLENLPEVLSFLGEIDSKGDAVFWAYLNGY
                     YFIFGDPASGIKQTSDGFLIYAYKLVKMCTPVQIDKFWLKIDKTGKIEVLGEKLFSRI
                     ENGCI"
     gene            complement(592..666)
                     /locus_tag="PLZ54_07950"
     tRNA            complement(592..666)
                     /locus_tag="PLZ54_07950"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(629..631),aa:Asp,seq:gtc)
     gene            971..1837
                     /gene="purU"
                     /locus_tag="PLZ54_07955"
     CDS             971..1837
                     /gene="purU"
                     /locus_tag="PLZ54_07955"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOJ66891.1"
                     /translation="MKGNDNTAILLIHCPDRPGILAAVTEFINRHQGNILYLDQYVNK
                     EEKVFFMRVEWDLKDFQIPREKIEEYFNTLIAIRFEMIFRLYFADEVPRMAIFVSKLS
                     HCLYDLLARYAAGEWKVEIPLIISNHKDLEPVAKRFGIDFYTFSINEKNKAEQEALEL
                     QLLKEKKVTFCVLARYMQILSPEFIQHYPNKIINIHHSFLPAFAGSKPYHAAYERGVK
                     IIGATGHYVTEDLDAGPIISQDVTHISHKDNVEELIRKGRDLEKIVLSAAVEKHINRK
                     ILTYKNRTIVFQ"
     gene            1856..3619
                     /locus_tag="PLZ54_07960"
     CDS             1856..3619
                     /locus_tag="PLZ54_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOJ66892.1"
                     /translation="MEISRLYDILDRYLEKFPNQDAALACKREGKWRKFSIQEYVDLT
                     NQISYGMLKLGIEPGDRIGIVSNSRPEWNMLDFAAMQIGAISIPIYATISQDDYRYIL
                     NHSEMKMIFIEGKELRLKVMPILPEIKSLKYVYTFVDQGTGLPFLEQLIQLGRENPAP
                     EKLQQLKAMQKPGDMATIVYTSGTTGNPKGVMLSHSNIVNQILSLHQIPAEWVKVALS
                     FLPLCHAYERMLVYLYQYLGISVYYAENLGTIGENIKEVNPNIMATVPRVLEKMYDKL
                     YMAGKKLPTISKFIYYRAVHFANKYQLEGMSWWSKKLHKFFDKMVYSKWREAIGGNFD
                     IVVSGGSALQPQMASFFSAIGMPVFEGYGLTETSPVIAVSQRVKNGRKFGTVGLPIPG
                     VEVKLGDQDEILCRGHNVMLGYYKDPELTASVIDPDGWFHTGDIGKFTPEGQLIITGR
                     IKSLFKTSNGKYVNPEAIETKFAESPFIENMIVVGENKKFPAALISPSFEYLKSWCEN
                     HKIPCGSPEEMIENEHVIKRFRDEVKHYNNFLGEFEQIKRFQLVAEEWTPQNGFLSPT
                     LKLKRNVIEEYYAEKIEKLFS"
     gene            3665..4423
                     /gene="truA"
                     /locus_tag="PLZ54_07965"
     CDS             3665..4423
                     /gene="truA"
                     /locus_tag="PLZ54_07965"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOJ66893.1"
                     /translation="MRRRYFIYISYKGTAYHGWQCQPNGISVQEVLIKTLSAILRTDI
                     NLVGAGRTDAGVHAKCMVAHFDYENEISDFSRFLLHLNSFLPPDIAVYKIVEVKPTAH
                     ARFDAVFRRYEYHVVFHKDVFKRELATRLFAELDFETMNKASEMLRNYQDFGSFCKLH
                     TDVKTNICHIHFAHWNQVEDEWVFTIQADRFLRNMVRAIVGTLFEVGRGKMTIEEFCS
                     VIEAGNRSKAGMSAPAEGLFLVDIQYPEEIFKLS"
     gene            4431..5186
                     /gene="hisF"
                     /locus_tag="PLZ54_07970"
     CDS             4431..5186
                     /gene="hisF"
                     /locus_tag="PLZ54_07970"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOJ66894.1"
                     /translation="MLAKRIIPCLDVKDGKTVKGINFENIKEVGDAVELGDWYAKRGA
                     DELVFLDITATNEKRKTLSELVARIARKINIPFTVGGGISSVEDVSLLLSCGADKISI
                     NTAAVKNPWLISELARQFGSQCVVLAIDTKYIDGEWYVFLNGGRIPTDIKTLDWAKKA
                     VALGAGEILLTSMNHDGTKDGFALDITCSVSESVNVPVIASGGAGKMEHFVDVFQKGK
                     ADAALAASIFHYREIDIPDLKKFLHENHIPVRW"
BASE COUNT         1638 a          934 c         1169 g         1660 t
ORIGIN      
        1 ttattgcata cacaaacacg aatgaatata atattcagac atgtgggttc catcaaacca
       61 ttaaaaaata aattatattc tgatgaaata agaagaaatt tatgtaccca ttcacggtat
      121 tatgaagttg tttttctaag ggagaaaaat tttattcgga ttcgttattt tcttcttcag
      181 gcaagggcat aaacaattca aacggagata tttgtttgtt tttcaacatt ttgacttatg
      241 atggaacaga ttatcgtttt ttggaaaatc ttccggaagt gttaagtttt ctgggcgaaa
      301 tagacagcaa aggagatgca gttttttggg catatttgaa tggttactat tttatttttg
      361 gagatccggc ttctggtatc aaacaaactt cggatggttt tttaatttac gcctacaagc
      421 tggtaaaaat gtgtaccccg gtgcagattg ataaattttg gctgaagatt gataaaaccg
      481 gcaaaattga agtgctgggc gaaaaattgt ttagcagaat agaaaacggc tgtatttaaa
      541 acacttattg atcatatcaa acaaaaaagc aacccttaaa ggttgctttt gcggtgcgta
      601 cgggactcga acccgtgacc ccatgcgtga caggcatgta ttctaaccag actgaactaa
      661 cgcaccaaaa atttattttt cacaaatttc ttttaaattc ttttctgctt tcctgttttt
      721 atttcggact caacaaccgt tgttgttcgg tgaaatttgc ggtgcaaagt tacgctaaat
      781 tttaatatct gcaatatctt tgtgaagttt tttgaatttt cttccattat tttctttgtt
      841 tggaagttac cgcacagaac gagaatacac atttcaatat ttcacaggtc aatgtttgta
      901 cagaagtctc gaaaacattt ttcctcaaaa agtattatat ttgcagacta aaaaaatttg
      961 aagaatcttt atgaagggaa atgataatac cgctatcttg ttgatacatt gtcccgacag
     1021 gcccggtatt ttagctgctg taaccgaatt tatcaaccgt catcagggaa atatacttta
     1081 tcttgatcag tacgtcaaca aagaagaaaa ggtcttcttt atgcgtgtgg aatgggattt
     1141 gaaagatttt cagattccga gggaaaagat cgaagaatat ttcaacacac ttattgctat
     1201 tcgatttgaa atgattttca gattgtattt tgctgatgaa gtgccgcgca tggctatttt
     1261 tgtttccaag ttgtcccatt gtctttacga tttgctggct cgttatgccg ctggagaatg
     1321 gaaagtggaa attcctttga taatcagcaa ccacaaagat ttggagccgg ttgccaaacg
     1381 ttttggaata gatttttata ctttttccat caatgaaaag aataaggcag aacaggaagc
     1441 actggaattg caactgttga aggagaaaaa agtaactttt tgtgtacttg cccgttatat
     1501 gcagatttta tcgcccgaat ttattcaaca ctatcccaat aaaattatta acattcatca
     1561 ttcttttttg ccggcttttg caggttccaa accttatcat gctgcttatg aaagaggagt
     1621 gaaaattatt ggtgcaacag gccattatgt taccgaagac ttggatgcgg ggcctattat
     1681 ttcgcaggat gtaacccata tttctcacaa ggacaatgtg gaagagctga ttcgcaaggg
     1741 tcgcgatttg gaaaaaatcg tactttcggc tgctgtagaa aagcacatta accgtaaaat
     1801 tcttacttat aaaaacagaa ccattgtttt tcaataattt ttaaaaatca atatcatgga
     1861 aatcagtcgt ttatatgata tattggatcg ttatttggaa aaatttccga atcaggatgc
     1921 tgcccttgcc tgcaaacgcg aaggaaaatg gcgcaaattc agcattcagg aatatgtgga
     1981 tttgaccaat caaatcagtt atggaatgct gaaactcggt atcgagccgg gcgaccgcat
     2041 aggcattgtg agcaatagcc gtcccgagtg gaatatgctc gattttgcag ccatgcaaat
     2101 tggagccata tcgattccta tttatgctac cattagtcag gatgattatc gatatatttt
     2161 gaatcattcc gaaatgaaaa tgatttttat cgaaggcaag gaattgcgac tgaaggtaat
     2221 gcctattttg cccgaaataa agtcgttgaa atacgtttat acttttgtag atcaaggtac
     2281 gggtcttcct tttttggaac agcttattca gttgggaaga gaaaatcctg ctcccgaaaa
     2341 attgcaacag cttaaagcta tgcagaaacc gggcgatatg gcaaccattg tttatacttc
     2401 aggaaccact ggaaatccca aaggtgttat gctttctcac agcaatattg tcaatcagat
     2461 tttaagtttg caccagattc cggccgaatg ggttaaagtt gctttgagtt ttctcccgct
     2521 ttgtcatgct tacgagcgaa tgctggttta tttgtatcag tatctcggaa tttcggtgta
     2581 ttatgccgaa aatttgggaa ccatcggcga aaatattaaa gaagtgaatc ccaacatcat
     2641 ggccactgtg ccgcgcgtgc tggaaaaaat gtacgacaaa ctctacatgg cagggaaaaa
     2701 gttgccgact atcagtaaat ttatttatta cagagccgta cattttgcca ataaatatca
     2761 gttggaaggc atgagctggt ggagcaaaaa attgcataaa tttttcgaca aaatggttta
     2821 ttccaaatgg agagaagcca ttggaggaaa tttcgatatt gttgtctccg gcggttcggc
     2881 tctgcaacca caaatggctt cgtttttttc ggctatcggc atgccggtgt ttgaaggtta
     2941 tggcttaacg gaaacttctc ctgtgattgc tgtcagtcag cgggtaaaaa acggcagaaa
     3001 atttgggacg gtgggactgc caattcccgg agttgaagtt aaacttggcg atcaggacga
     3061 gattctttgt cgggggcaca atgtgatgct gggatattac aaagatcccg agttgaccgc
     3121 cagcgttatt gaccctgatg gttggtttca taccggcgac ataggaaaat ttacacctga
     3181 aggccaactg attattaccg gacgaataaa aagcctcttt aaaacttcaa acggtaaata
     3241 tgtgaatccc gaagccattg aaacaaaatt tgcagaatct ccgtttatag aaaacatgat
     3301 tgttgtgggt gaaaacaaaa aattccctgc tgcactcatt tcgccaagtt tcgagtattt
     3361 aaaatcgtgg tgcgaaaacc ataaaattcc ttgtggcagt ccggaagaaa tgattgaaaa
     3421 cgagcacgta ataaaacgtt ttcgggatga agtgaagcat tacaacaatt ttcttggcga
     3481 atttgaacaa ataaaacgtt ttcagttggt tgccgaagaa tggactccac aaaacggatt
     3541 cctttcgcca actttaaaat tgaaaagaaa cgttattgag gaatactatg ccgaaaaaat
     3601 tgaaaaattg ttttcgtgat aattgcaagt ttttcttatt ttctgaacta ttcagttttt
     3661 tggcgtgaga aggcggtatt ttatttatat ttcttacaaa ggaacggcct atcacggttg
     3721 gcagtgccag cccaacggta tttcggttca ggaagtattg ataaaaacgc tgagtgccat
     3781 tttgcgaacg gatattaatt tggttggtgc cggtagaacc gatgccggtg ttcatgccaa
     3841 atgcatggtg gcacattttg attacgaaaa tgagatcagc gatttttcaa ggttcttatt
     3901 gcatctcaac agttttttgc ctccggatat agcggtttat aaaattgttg aagtaaagcc
     3961 taccgctcat gcccgtttcg atgccgtttt caggcggtac gaatatcatg tggtttttca
     4021 taaagatgtt ttcaaacgtg agctggccac ccgcctgttt gccgaattgg attttgaaac
     4081 gatgaacaag gcatcagaaa tgctacgcaa ctatcaggat tttgggagtt tttgcaagtt
     4141 gcatacggat gttaaaacca atatttgcca tattcatttt gcacattgga atcaagtgga
     4201 agatgaatgg gtgtttacca ttcaggccga ccgttttctg cgaaatatgg tacgagccat
     4261 tgtgggaacg ctttttgagg tgggaagagg aaaaatgacc attgaagaat tttgttccgt
     4321 aattgaagcc ggaaatcgca gcaaagcagg catgtcggct ccggcagaag gattgttttt
     4381 ggtggatatt caatatcctg aagagatttt taaactgtca taaaaaattt atgttggcga
     4441 aacgcattat tccctgtttg gatgtaaaag atgggaaaac cgtaaaagga attaatttcg
     4501 agaacatcaa ggaagttggt gatgctgtag agttgggcga ttggtacgct aaaaggggag
     4561 cggatgagtt ggtctttttg gatattacgg ccacgaacga aaaacgcaaa acgctttcgg
     4621 aattggtagc acgcattgcc cgtaaaatca atattccctt taccgtcggt ggcggcattt
     4681 ccagtgtgga agatgtatcg ctgctacttt cgtgcggagc agataagatt tctatcaaca
     4741 cggcagccgt aaaaaatcct tggttaattt ccgaattggc acgacagttt ggaagtcaat
     4801 gtgtagtatt ggccatcgac accaaatata tcgacggcga atggtatgtt tttttgaatg
     4861 gtggaagaat tccaactgat attaaaaccc tcgactgggc aaaaaaagct gtggcgttgg
     4921 gagccggaga aattttgctg acttccatga atcacgatgg tacgaaagat ggttttgctt
     4981 tggatattac ttgttcagta tccgaatcgg taaatgttcc ggtaatagcc agtggtgggg
     5041 cagggaagat ggaacatttt gtcgatgttt ttcaaaaagg aaaagctgat gctgcgctgg
     5101 ctgccagtat ttttcattat cgggaaattg atattcccga cttgaaaaaa tttcttcacg
     5161 aaaatcacat tccggtaaga tggtaagaaa tttataatgg gaaattttgg gtcggaataa
     5221 gatcgtcctc acgctgccgc acaaattctt tcgtgtaaca aaaattgtaa atcaacatca
     5281 ttccttgtaa acatatccat ccgttctgca aatgcaaaac ttacaaaata tacttcttca
     5341 ggattgtgaa atttataatt tctactcaag tttcaaagat agttgatttt ttaaaaataa
     5401 c
//
LOCUS       DAOSCH010000121         5341 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_9720_length_5341_cov_5.631858, whole genome shotgun sequence.
ACCESSION   DAOSCH010000121 DAOSCH010000000
VERSION     DAOSCH010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9720_length_5341_cov_5.631858"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..232)
                     /locus_tag="PLZ54_07975"
     tRNA            complement(149..232)
                     /locus_tag="PLZ54_07975"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(196..198),aa:Leu,seq:caa)
     gene            complement(358..1566)
                     /locus_tag="PLZ54_07980"
     CDS             complement(358..1566)
                     /locus_tag="PLZ54_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HOJ66895.1"
                     /translation="MKITSGNDNKIRKFFAEKEFPMIAKTFQGLEEVLAEELTELGAN
                     NVQIERRAVSFSGDKAMLYKANLHCRTALRILKPIFSFEADNTDELYKKIKLVEWDVL
                     LHVNNTFSVDATVFSDEFQHSRFVTYRVKDAIVDWFSENFNDRPSVSVEDPDFVFNIH
                     IAGSHCTLSLDSSGESLHKRGYRTGQTKAPLNEVLAAGMLMLAGWKGQSDFLDPMCGS
                     GTLLIEAALIALNIPPGIFRASFAFEKWPDFDKVLFDDLYNDDSQELLFEHHIFGSDI
                     SVSAIKIAQQNVKSSGLSKYISLKVLPLEKLEPPSSDCFIVTNPPYGERLPSKDIFEL
                     YANLGSLLKRKFSGCTAWVISSNEEALKKIGLKPSKRYRLMNGPLECWFQQYELFSGK
                     RNDYLQKKNS"
     gene            complement(1577..2467)
                     /locus_tag="PLZ54_07985"
     CDS             complement(1577..2467)
                     /locus_tag="PLZ54_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOJ66896.1"
                     /translation="MEKEILDAIIELSKIDQYKFACHQHVRNERMPSINNLQEIVQLC
                     RSVLFPGYFDDAALDSDTLLYHIGLSIDRLKLLLTEQLKAGFCFEREHSTDKNLCSDM
                     EQNVVQKVNAFIAQLPEIRRKLYTDVIAAYNGDPAAKSIGEVIFCYPGIRAISNYRIA
                     HALLILDVPLIPRIISELAHSETGIDIHPGAEIGDYFMIDHGTGVVIGETSVIGNNVK
                     MYQGVTLGARSFPLDENGNPIKGIVRHPRIGNNVIIYSNSTILGNITVGDGAVIGGNL
                     WVDSDVPAGAKLSQDDKLKR"
     gene            complement(2486..3022)
                     /locus_tag="PLZ54_07990"
     CDS             complement(2486..3022)
                     /locus_tag="PLZ54_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ66897.1"
                     /translation="MKNMLRVAILGPESTGKTELAKSLAEYFQVDWVPEFARAYVESL
                     SRPYEYEDVCRIAAHQIDEELFYENHPSDGKKFVFFDTDLIITKVWFDYKYGKVPKFL
                     QERLNCHFFDFYLLCYPDLPWQPDSVREHGDDREFFFNWYKQEIEKTGKSVSIITGIG
                     NERLQNAIRALTSFVSYL"
     gene            complement(3039..3662)
                     /gene="pnuC"
                     /locus_tag="PLZ54_07995"
     CDS             complement(3039..3662)
                     /gene="pnuC"
                     /locus_tag="PLZ54_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOJ66898.1"
                     /translation="MILLKETFSLNPVIEYVIDNWVELTGAILSLIYLILSVRQKIGL
                     WIFGFLCAALYVYVFFQSKFYAGMTLQFYYLAVSIYGWITWKRGKTDTQPELPVTRTS
                     KHMAFNLLTVFLAILVIYFFILSNFTDSPLPRGDAFTTALSIVATWMLAKKLLENWLL
                     WIIADAVSTGLYLYRGLYPTAVLFTVYTLMAVLGYFQWKKSMKHQTH"
     gene            complement(3676..5259)
                     /locus_tag="PLZ54_08000"
     CDS             complement(3676..5259)
                     /locus_tag="PLZ54_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOJ66899.1"
                     /translation="MKKLYLVFIFNLILAGVFGQGISLMQQRKLMNALNAISSLYVDS
                     INDKKLIESAITALLKDLDPHSSYIPREEVERINEPLEGEFEGIGIQFQIFEDTLMVV
                     QTIAGCPAEKVGILPGDRIIYINDELVAGVKIQNSDILKRLRGPRGSEVTVKVLRRGK
                     PELLIFKIIRDKIPLYSVDAQYMIGNDIGYLKINSFGRTTDEEFVKAFTELQKKGMKH
                     LIVSLQGNGGGYLATAIQLADQFLDRGKLIVYTEGFHQPKKESLATAVGKYETGKLVI
                     LVDEYSASASEIVSGALQDWDRAVIVGRRTFGKGLVQQQLPLVDGSMMRLTTARYYTP
                     SGRCIQKPYKDGADKYERELLTRYKKGELIHPDSIHFPDSLRYQTLRLKRTVYGGGGI
                     MPDVFVPLDTTRFTDYHQKLVARGVINKVSVQFIDQNREDLKRKFSTFDKFEKEFTVD
                     EAFLQQLIQAGEKENIPFQSSQFDTSRELIKLQLKALIARDLWDMNAYYRIINNDNEV
                     LKKAVELLQTPNAYEQILK"
BASE COUNT         1634 a         1181 c          975 g         1551 t
ORIGIN      
        1 ctcttcagat tgactaatat ctatgcttag acacatgttt ttgcaggtga gccataaaat
       61 tgctccgcaa aaatcatcat tttgttgaaa tgttacaacc ataaaaataa aatcagcaga
      121 aaatcaaatg attaactgct gattaaagta cccagagccg gggtcgaacc ggcatggaag
      181 tgaatccact ggtgtttgag accagcgcgt ctaccgattc cgccatctgg gcattagcgg
      241 gtacaaaagt aaattatttt ttttattccc aaaagcaaaa atgccgaaat ttattaattt
      301 caatcaaaaa agaaacttta ataactaata ttcaaaagat tataaataca actggaatca
      361 ggaattcttt ttctgcaaat aatcgtttct tttacccgaa aaaagttcat actgctgaaa
      421 ccagcattcc aaaggtccgt tcattaagcg ataacgtttt gaaggtttta aaccaatttt
      481 tttcagtgct tcctcattgg aactgatcac ccaagcagta caacccgaaa atttacgttt
      541 cagcaatgat cccaagttgg catataattc aaaaatgtct tttgatggga gcctttcgcc
      601 ataaggtgga ttggtaacaa taaaacaatc ggatgaaggc ggttcaagct tttccaacgg
      661 cagtaccttt aaagaaatat attttgacag tccgctactt ttaacattct gctgggcaat
      721 tttaatggca gatactgaga tgtccgatcc gaaaatatga tgttcaaaaa gtagctcctg
      781 tgaatcatca ttatataaat catcaaaaag caccttatcg aaatcgggcc atttttcaaa
      841 agcaaaagaa gctctgaaaa taccgggagg gatattcaag gcaatcaagg ctgcctcgat
      901 aagcaaagtt ccggagccgc acatcggatc aagaaaatcg gattgtcctt tccagccggc
      961 caacatcaac attccggccg ccaaaacttc attcagcgga gcttttgtct gtccggttct
     1021 ataaccgcgt ttatgaagcg attcaccgga actgtcgagc gacaaagtac aatggcttcc
     1081 ggcgatgtga atattaaaca cgaaatcggg gtcttcaacc gaaacacttg gccgatcgtt
     1141 aaaattctct gaaaaccagt caactatggc atctttcacc cgataggtta caaaacgcga
     1201 atgctgaaat tcatcagaaa aaacggttgc atcaaccgaa aaagtattat ttacatgcag
     1261 aagaacgtcc cattccacca actttatttt tttgtataat tcgtctgtat tgtcggcttc
     1321 aaaagaaaaa atcggtttaa gaatacgtaa ggcggttctg caatgcaaat ttgctttgta
     1381 caacattgcc ttatcaccgc taaagctcac tgcgcgccgc tctatttgca cattgttggc
     1441 tcccaattcg gtaagttctt cagccaatac ctcttcgagt ccctgaaagg tcttggcaat
     1501 cataggaaac tccttctcgg caaagaattt cctgatttta ttgtcgtttc cggaagttat
     1561 tttcatcggt taacaatcaa cgtttcaatt tatcgtcctg cgaaagcttt gctccggccg
     1621 gaacatcgct atccacccaa agattaccgc caattacggc accatcacca acggtgatat
     1681 ttcccaaaat ggtagaattg gaataaatga ttacattgtt gccaattcgt ggatggcgaa
     1741 cgataccttt tatgggattt ccattctcgt cgagcggaaa acttctggct cccagcgtaa
     1801 cgccttgata cattttcacg ttgtttccaa tcaccgaagt ttctcctata accacacctg
     1861 tgccatgatc tatcataaaa taatctccaa tttcggctcc gggatgaata tcgataccgg
     1921 tttccgaatg agccagctca ctgataatac gtggaatcaa aggaacatcg agtatgagta
     1981 gcgcatgagc aatacgataa ttgctgatgg ctcgaattcc cggataacaa aaaatcactt
     2041 ctccaatact tttggcagca gggtcgccat tgtaagcggc aatgacatcg gtataaagtt
     2101 ttctgcgaat ttcaggcaat tgagctatga aagcattcac tttttgaaca acgttctgct
     2161 ccatgtcgct gcataaattt ttatctgtgg aatgttctct ttcgaaacag aatcccgctt
     2221 tcaattgctc ggtaagcaaa agtttcaatc gatcgatact aagcccgata tgatagagca
     2281 acgtatcgga atcgagcgct gcatcgtcaa aatagcccgg aaacaaaaca gaacggcaca
     2341 actgaacaat ttcctgcaaa ttattgatgg aaggcattcg ctcatttcta acatgctgat
     2401 gacatgcaaa tttatactga tctattttcg ataattcgat gatagcatct aaaatttcct
     2461 tttccataaa tttgaataat aaaatttata aataactcac aaaagaagtc aaagcccgaa
     2521 tggcattttg gagcctttca ttgccgattc cggtgataat agaaacagat tttcctgttt
     2581 tttctatttc ttgcttatac cagttgaaaa agaattctct gtcgtctcca tgttcacgaa
     2641 ccgaatcggg ttgccacggc aaatcgggat aacacaacaa ataaaaatca aaaaaatggc
     2701 aatttaatct ttcctgtaaa aatttgggta ctttgccata cttatagtca aaccatactt
     2761 tagtgataat taaatcggta tcgaaaaaaa caaatttttt cccatcgctc ggatgatttt
     2821 cataaaacag ttcttcatca atctgatggg cggcaatgcg acaaacatcc tcatattcgt
     2881 acggccggga taaactttcc acgtatgcgc gagcaaattc aggtacccaa tccacctgaa
     2941 aatattcggc aagggatttg gcaagctcgg tttttccggt agattcagga cccaaaatag
     3001 caactctcag catattcttc atttacttca aatacatttt aatgtgtctg atgtttcatg
     3061 gactttttcc actgaaaata gcccaacacg gccatcagcg tataaacagt aaaaagtacg
     3121 gctgtaggat aaagcccgcg ataaagatac aaacctgttg aaacggcatc agcaataatc
     3181 cacagtaacc aattctcgag caattttttt gcaagcatcc acgtagccac tatactcaag
     3241 gctgtggtaa aagcatcacc tctgggaagt ggtgaatcgg taaaattact taaaatgaaa
     3301 aaataaatca ccaaaattgc caaaaaaacc gtcagcaaat tgaaagccat gtgtttggaa
     3361 gttcgtgtta ccggcaattc gggttgggta tccgtttttc ctcttttcca agtaatccat
     3421 ccgtaaatgc tcactgcaag gtaataaaat tgaagcgtca tgccggcata aaatttactt
     3481 tgaaaaaaaa catatacgta caatgccgca cacaaaaatc cgaaaatcca cagcccgatt
     3541 ttctgcctca ccgataatat cagatatatc aggctcaaaa tagcgccggt gagttctacc
     3601 caattgtcga taacatattc tatgacagga ttcaacgaaa aagtttcttt caataaaatc
     3661 attgctttga cctttttact ttaaaatttg ttcgtacgca ttcggcgtct gcaataattc
     3721 gactgctttt ttcagcactt cgttgtcgtt gtttataatg cggtaatagg cgttcatatc
     3781 ccacaaatcg cgtgcaatca atgccttaag ttgcagtttg atcagctctc tcgatgtgtc
     3841 aaactgagag gactggaacg gaatattttc cttttctccg gcctgaatca gttgctgcaa
     3901 aaaggcttcg tcaaccgtga attccttttc aaatttgtcg aacgtactaa attttctctt
     3961 caaatcctcc ctgttctgat cgataaactg aacggaaact ttattgatta ctccccttgc
     4021 aaccaacttt tgatgataat cggtaaaacg tgtggtatcc agcggaacga aaacatcggg
     4081 cataatacct ccaccgccat aaacggtacg tttaagtcgc aaagtctgat aacggagtga
     4141 atcggggaaa tgaatgctgt cgggatggat taattctcct tttttgtaac gggtcagcaa
     4201 ttcgcgctca tatttatcag caccatcttt ataaggtttc tgaatgcaac gccccgatgg
     4261 cgtataatat cgtgctgtcg tcaacctcat catcgagcca tcaaccaacg gaagctgttg
     4321 ctgcaccaat cctttgccga aagttcttcg tccaacaatg actgctctgt cccaatcctg
     4381 caaagctccc gaaacgattt cgctggctga agccgagtat tcgtcaacca gaataaccag
     4441 ttttccggtt tcgtattttc ccacagcggt agcaagcgac tctttcttgg gttgatggaa
     4501 tccctctgtg taaacgataa gttttccacg atccaaaaat tgatcagcca gttgaatagc
     4561 cgtagccaga tagccgcccc cattcccctg caagctgaca atcaaatgct tcattccttt
     4621 tttttgaagt tcggtaaatg ccttgacaaa ttcttcatcg gttgtccgac caaaactgtt
     4681 gattttgagg tatcctatat cattcccaat catatactga gcatccacgc tgtagagggg
     4741 aattttatcg cgtataattt tgaagatgag caattcgggt tttccacgcc gcaaaacttt
     4801 gactgtaact tccgaacctc gcggcccacg caagcgtttc aaaatgtcgg aattttgaat
     4861 ttttacaccc gccaccaatt cgtcgttaat ataaataatt cggtcgccgg gaagaattcc
     4921 tactttttcg gccggacatc ctgcaatagt ttgaaccacc atcaaggtgt cttcaaaaat
     4981 ttgaaactga atgcctattc cttcaaactc gccttccagc ggttcattga tacgctccac
     5041 ttcttcacgt ggaatgtagg atgaatgcgg atccaagtcc ttcaacaggg cagttatggc
     5101 cgattcgata agttttttat cgttaatgct gtccacatac aggctggata tggcattcaa
     5161 ggcattcatt aattttcgtt gctgcatcaa cgatatgccc tgcccgaaaa ctccggcaag
     5221 aattaaattg aaaataaaaa ccaaatataa ctttttcatt ttttgttggt tatgtcttaa
     5281 gtaaaaaaag agaacaataa taatcaaaca atttttgttt caaagaatta atttttttcg
     5341 g
//
LOCUS       DAOSCH010000122         5315 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_9773_length_5315_cov_6.792015, whole genome shotgun sequence.
ACCESSION   DAOSCH010000122 DAOSCH010000000
VERSION     DAOSCH010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9773_length_5315_cov_6.792015"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="PLZ54_08005"
     CDS             complement(<1..661)
                     /locus_tag="PLZ54_08005"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOJ66900.1"
                     /translation="MNKIEQLIQQYCPNGVEYKKLGELGIFYGGLTGKSKTDFQNGNA
                     KFITYMNVYSNIAVKTNINDFVRIGENENQNRVEYGDVLFTGSSETPDECGISSVLTE
                     NISEPLYLNSFCFGFRLHDKELFLPDFLKYLFRDDKIRKQIAQTANGVTRFNVSKKRF
                     AKILIPIPPLPVQQEIVNILDKFTELEAKLEAELEARRKQYEYYRNQLLTPVEVDGKW
                     LM"
     gene            complement(654..1001)
                     /locus_tag="PLZ54_08010"
     CDS             complement(654..1001)
                     /locus_tag="PLZ54_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66901.1"
                     /translation="MSKIDELIAQYCPNGVEFKKLWEVLDYEQPNKYNGLIIELQQTG
                     ESLPLNVINDLKTNEQIPPFSRNDDSPCGEMREGSGGFAATSFSSIFYNEAVIPNGTK
                     CSEETVTKKTKNE"
     gene            complement(1074..2636)
                     /locus_tag="PLZ54_08015"
     CDS             complement(1074..2636)
                     /locus_tag="PLZ54_08015"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HOJ66902.1"
                     /translation="MTKEQERDELHRTIWQIANDLRGSVDGWDFKSYVLGMLFYRFIS
                     ENLTSFLNEEERRAGNPDFDYASISDEEAEFGRSETVKEKGFYILPSELFENVRRNAK
                     NDPNLNETLSRVFTNIEDSAKGSPSEKNFKGLFDDIDVNSNKLGSTVLKRNEMLVKIM
                     DAIGGLQLGDYQDNSIDTFGDAYEYLMTMYASNAGKSGGEFFTPQEVSELLARIAVVG
                     KTEVNKVYDPACGSGSLLLKFAKVLGKENVRLGFFGQEINLTTYNLCRINMFLHDIGY
                     EKFDIAHGDTLTDPKHWDDEPFDAIVSNPPYSIRWEGDSNPVLINDPRFSPAGVLAPK
                     SKADLAFTMHMLSWLSTTGTAAIVEFPGVLYRGGAEQKIRKYLVDNNYVDAVIQLPPD
                     LFFGTSIATCVIVLKKSKNDNATLFIDASAEFVRGGNKNKLSEANREKILDAYITRSD
                     IDYFARLVPNGDIAENEYNLAVSSYVEQENTAEEVDIEKLNARIAGIVIRQQKLREAI
                     DEIVADLEGGEA"
     gene            complement(2707..4500)
                     /locus_tag="PLZ54_08020"
     CDS             complement(2707..4500)
                     /locus_tag="PLZ54_08020"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOJ66903.1"
                     /translation="MRKGEIIECNNYSYSLIELIGNGGSGEVWKAAANNEYYAIKFLH
                     ADETKKVIRFENELKFCKENPHKNICPVIAEGEHNGKRFYVMPLYEKTLREIICEEKD
                     PDLLVKYILQLCAAVKFAHKKGVIHRDIKPENILISERKLVLADFGIAHFKDSILTKS
                     NNLLANRNYLAPEQKIKDNAKNIDKSADIYALGLVINECFTKQNPAGSSFKVIADDYP
                     LYFKLDTLVENMTKQNPNERFTIEDVIVELKYIHGTIKQHLQEIQAILLKDEYPNDIS
                     KKTLNKIINRASEDILFAKMVFETKSLEEINKINLNWHKKIGYNVSPLFFNLDVQEKV
                     LALCKNKFVYESNYYGPSRYYTPLNLKDNDEHKQLYLQLSNFIAQYPLKHKHGKFLDL
                     SGQILKYFASCEDYHCREIMNQIKNNNFLKEAEYNLIDAPVLWIVRELKNTIVENMDG
                     IIEGFSSIFGIQYKFDIAEHISINWSRTIDYETYDDDELMSESHLDNEKQIKKILLEF
                     QQQWQIIFNKIDETYYSIKFKSYRQFERFKKHVHKVANSHEKDGPIKNDILNIVKECN
                     CANGIVEIKLSLVFDIPLPLQIILGLKTNYQ"
     gene            complement(4648..4782)
                     /locus_tag="PLZ54_08025"
     CDS             complement(4648..4782)
                     /locus_tag="PLZ54_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66904.1"
                     /translation="MMLFANKIRQLRIEKLFQRQLAAALEIDMPMYNKIQRGTRMAKS
                     "
BASE COUNT         1505 a         1027 c          941 g         1842 t
ORIGIN      
        1 tcatcaacca tttgccatcc acttctacgg gggttaataa ttggttgcgg tagtattcgt
       61 attgtttacg ccgtgcttct aattctgctt ctaattttgc ttctaattct gtaaatttat
      121 cgagaatatt tacaatttct tgttgaacag gaagaggtgg aatgggaatg agaatttttg
      181 caaatcgttt ttttgataca ttaaatctgg taactccatt tgcagtttgt gcaatttgct
      241 ttcttatttt atcgtctctg aatagatatt tgagaaaatc gggtaaaaat aattctttat
      301 catgtagtct aaaaccaaag cagaaactgt ttaaatacaa tggctctgaa atattttcag
      361 ttaatacaga cgaaattccg cattcatcag gtgtttcgga agaaccagtg aataatacat
      421 ctccgtattc aaccctgttt tgattttcat tttctccaat tctgacaaaa tcatttatat
      481 tcgtttttac agcaatgtta gaataaacat tcatgtatgt tataaatttt gcattcccat
      541 tctgaaaatc agttttactt ttacctgtaa gccctccata aaaaatacct aattctccta
      601 attttttata ttctacacca ttagggcaat attgttgtat gagttgttct attttattca
      661 tttttagttt ttttagttac agtttcctca ctacactttg ttccgttcgg aatgacggct
      721 tcattataaa aaatggaaga gaaggaagta gcggcgaagc cgccacttcc ttctctcatt
      781 tccccacacg gcgaatcgtc attccgactg aaaggaggaa tctgctcatt cgttttcagg
      841 tcattgatta cattcaaggg aagactctct cctgtttgtt gcaactcaat aattaaacca
      901 ttatatttat ttggttgttc ataatccaat acttcccaca atttcttaaa ctctacacca
      961 ttagggcaat actgtgcaat cagttcatcg atcttactca ttttctttcc ctcccctttc
     1021 aatctttttc tgaacagctt tgatgctttc aagataggct ttttctaccg gattcatgct
     1081 tcacctcctt ccaaatcggc tacgatttcg tcgatagctt cacgaagttt ttgctgacgt
     1141 atcacaatcc cggctatgcg ggcgttgagt ttttcgatgt ccacttcttc ggccgtgttc
     1201 tcctgctcca cgtagctgct tacagccagg ttgtactcgt tctcggcaat gtcgccattg
     1261 ggaaccagtc gggcaaagta gtcgatatcg ctacgagtaa tatatgcatc gagaatcttt
     1321 tcgcgattgg cctcgctgag cttgtttttg ttgcctccgc gtacgaattc ggccgaagcg
     1381 tcgataaaaa gtgtggcatt gtcattttta ctctttttaa gtacaataac acaagttgcg
     1441 atactggtac caaaaaacaa atcgggaggc agttgaatga cggcatccac ataattgttg
     1501 tcgacaaggt atttgcgaat tttctgctcg gcaccgccac gatagagcac gccgggaaat
     1561 tctacaatgg ctgccgtccc ggttgtggag agccagctga gcatgtgcat ggtgaaagcc
     1621 aaatcggctt tgctcttggg agcgagaact ccggcgggag aaaaacgggg gtcgttgatg
     1681 agcacagggt tgctgtcgcc ttcccatcgg atggaataag ggggattgga aacaatggca
     1741 tcgaaaggtt cgtcgtccca atgtttggga tcggtaaggg tgtcgccgtg ggctatgtcg
     1801 aatttttcgt atccgatatc gtgcaaaaac atgttgatgc ggcaaaggtt gtaggtggtg
     1861 aggttgattt cctgtccgaa aaaacccaac cgaacgtttt ctttaccgag gactttggca
     1921 aatttgagca aaagggaacc cgagccgcag gcgggatcat ataccttgtt gacttcggtt
     1981 ttccccacca cggcaatccg ggcaaggagt tcggaaactt cctgaggagt gaaaaattcg
     2041 cctcccgatt tgcccgcatt gcttgcatac atggtcatta ggtattcgta ggcatcgcca
     2101 aaggtatcga tggaattatc ctgataatcg cccagctgca aaccgccgat ggcatccatg
     2161 atcttcacca gcatttcgtt ccgtttgaga acggtggagc cgagtttgtt gctgttgaca
     2221 tcgatatcgt caaacaagcc tttgaagttt ttttcactcg gggaaccttt cgccgaatct
     2281 tcgatgtttg tgaaaacacg actcagcgtt tcgttcaggt tgggatcgtt tttggcgtta
     2341 cggcgcacat tctcaaaaag ctcggaaggc agaatataaa aacctttttc ttttacggtt
     2401 tcgctccttc caaattcggc ttcctcgtcc gaaatgcttg catagtcgaa gtcgggattg
     2461 cctgcccgcc gttcctcttc attgaggaag gaagtgagat tttcggatat gaagcggtaa
     2521 aacaacatgc ccagcacgta cgatttaaaa tcccagccat cgacactgcc tctgaggtcg
     2581 ttggctattt gccaaatggt tcggtgaagt tcgtcgcgtt cctgctcttt ggtcataaaa
     2641 gtgagttata taatttaatt ttttattatg tttaatgcct tgtgatatca aaaaaagaat
     2701 aatttattat tgataattag tctttaatcc aagaataatc tgtagtggca gtggaatatc
     2761 aaacaccaat gaaagtttta tctctactat cccattagca caattacatt ccttgacaat
     2821 gttcaaaata tcatttttga taggtccgtc tttttcatga gaatttgcta ctttatgaac
     2881 gtgctttttg aatctttcaa actgtctata gctcttaaat tttattgaat aatatgtttc
     2941 gtcaattttg ttgaatatta tttgccactg ctgttggaat tccaaaagta tttttttgat
     3001 ttgtttttca ttatccaaat gactctcaga catcaattca tcatcatcgt aggtttcata
     3061 atctattgtc cgactccagt ttatcgaaat atgttcagct atgtcaaatt tatattgtat
     3121 accaaaaata cttgaaaaac cttcaatgat accatccatg ttttccacaa tagtattttt
     3181 caactcacga actatccata aaacaggagc atcgatcaga ttgtattcag cctcttttaa
     3241 aaagttgtta ttcttaattt gattcataat ctctctacaa tgataatctt cgcaggaagc
     3301 aaagtatttt agtatttgtc cagataaatc taagaatttc ccatgtttgt gcttcaatgg
     3361 atattgagca ataaaattac ttaattggag gtacaattgt ttatgctcat cattatcttt
     3421 caaattcaga ggagtgtaat atctgctagg gccataataa ttactttcat aaacgaattt
     3481 atttttgcac aaagccaata ccttttcctg aacatctaga ttaaaaaata aggggctcac
     3541 attatatcca atcttcttgt gccaattcag attgatttta tttatttctt caagagattt
     3601 agtttcaaaa accattttag caaataaaat atcttcgctt gctctattaa tgattttgtt
     3661 caacgttttt ttactaatat catttgggta ttcatctttc agtagtatag cctgaatctc
     3721 ttgtaaatgc tgcttgattg taccatgaat atattttagt tcaacaataa catcctcaat
     3781 tgtgaatctt tcgtttggat tttgtttggt catattttct accaaagtat ccagtttgaa
     3841 atataatgga taatcgtcag ctataacttt gaaactggat cctgccggat tttgcttggt
     3901 aaagcattca ttaatcacta atcctaacgc ataaatatcg gcggatttat caatgttttt
     3961 agcattatcc tttattttct gctcaggggc taaataatta cggttagcca acaaattgtt
     4021 tgactttgtg agtatcgaat ctttgaaatg agcaattcca aaatcagcaa ggactaactt
     4081 tctctctgag attaagatat tctccggttt gatatctcta tgaataacgc ctttcttatg
     4141 tgcaaacttc actgcagcac acaattgcag aatatacttt actaatagat caggatcttt
     4201 ttcttcacaa ataatttctc taagagtttt ttcataaaga ggcattacat aaaagcgttt
     4261 accgttatgc tcgccttctg ctataacagg acaaatattt ttatgaggat tttctttgca
     4321 gaatttcagt tcattttcaa atcggataac ctttttcgtt tcgtcagcat gcaaaaattt
     4381 tattgcatag tattcgttat ttgcagcagc tttccaaact tcaccagagc ctccatttcc
     4441 aattagctca attaacgaat aactataatt attacattca ataatttctc cctttctcat
     4501 tttgaaaatc tgtttttatt ctcttttgca acctctaaaa ccgtcacaaa caattctttc
     4561 tctcccaccc cctactatat ttgccagcca aagggtaatt aactatgttc cgtcgttagg
     4621 aagcaattga gtaaagtcaa ttacctacta agactttgcc atacgggttc cccgctgaat
     4681 tttgttatac atcggcatgt ctatctctaa tgccgctgcc agttggcgtt ggaacagttt
     4741 ttctattctt aattgtcgga ttttattagc gaacagcatc atggttatgt ttttttactt
     4801 tttatcttct caagattttc agaataagca aagataaaca attattcaca taaaaaaaca
     4861 ggtataaaag aaaagatttc aacatataaa aaggacaaag agaatatttg tttttgataa
     4921 cttatgacaa aaagaagcaa cttaaaatag ttgcttttga atgtgtggaa ttttatggtt
     4981 ttttgtactt gtcatgtgca taatgaatta tgaggttttg ggaggaaagg cgttttatgt
     5041 agaatgcttt tcggaaacca tcggagaagc tactaaaaaa tggacaatcc ctattcaaaa
     5101 ttggggcatt attcttaatc aatttattct tatctttggc gaaagaataa gactttaatt
     5161 tttatcccaa ctttttaatt tacacacttc gtgaaatagt gtccgattct aattttcaca
     5221 gatataaaga tgtgttgttc caaatatcaa agatataaaa tttgaaagca aatcacaact
     5281 caggataatg tttggaaaag tttctcgata acgtt
//
LOCUS       DAOSCH010000123         5268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_9886_length_5268_cov_5.834069, whole genome shotgun sequence.
ACCESSION   DAOSCH010000123 DAOSCH010000000
VERSION     DAOSCH010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9886_length_5268_cov_5.834069"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..1930)
                     /locus_tag="PLZ54_08030"
     CDS             complement(221..1930)
                     /locus_tag="PLZ54_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOJ66905.1"
                     /translation="MHGFNVLISKKAIKQQPHFCWKKPFDFQQKLALRTFSDEHIVVE
                     QFTGTKFISEKLWLDNEDFFILTDGVITNLKALCLEHKAANYDALLRKIVKEKSFFKN
                     FRGHFVGFVYFKKEKTCIAFNNHTASKKLFYFADQNTIVIGTDLYTLSKTLETEKIKR
                     TLDKEAAVFLLTSGFMHENLTLLEEVKQLRAGEFLQIGNFNLKTDFYFHLQDIQENHD
                     AKSDIVEQLDFLFKKSIDNEFSLDKEHQLISLTTLSGGLDSRMVALTALQLGFSNQMF
                     LNFSAKGYADEIIARQISEQYQVPLETFSIEAESLKPLEEVVLVNDGLTIYTGASAVF
                     DAIKRHRITNTGIIHTGMLGDALLGSYLISDHKTKPQISDGVYSTQLLNKYAPVFQKY
                     IQNYSDIEMYKFYNRGFSGMNNGFLYFDLCGESDAAFLNPDFLSYALSIPAKYRYKEQ
                     IYIDWIKIKHPEFTGFTWESIGGKPTNNSILRCYYRYRRALVKRMPLQTMWKNNMNPE
                     QLWYDKNLSLRQTLDQYFLENIHLLSSCSDLFDDCQFLYKKGNITEKAQVLTLLAACK
                     LLL"
     gene            complement(2034..3200)
                     /locus_tag="PLZ54_08035"
     CDS             complement(2034..3200)
                     /locus_tag="PLZ54_08035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ66906.1"
                     /translation="MIKTLFISAWYPNRYDEMFGLFVRKHAEAVSLYCDVQVLYVHPS
                     SDIRHFETEIRQNGKVKETIVYFPTSKSGVFSKLLKQINFLRAYLKGWRMLQKSGFRP
                     DIVHANILTRTVVMAYGIHLFTGIPYVITEHWTRYLPLRKSYNGLLRKKITEFVVKHA
                     AAVFPVSENLKNAMISNKLWNKNYFVVNNAVDDVFLTDISKVSETKKIILHVSCFDDQ
                     QKNVKGLLNSLKALSVNRNDFKMIFIGTGVDFKEVVDYSVKLNFPEDMIHFVGEKNPA
                     EVAEYMKNADFFVLFSNYENSPVVISESLVCGKPVISTDVGGIAEHINETNGILIPAR
                     DENMLFESLNFMLDHFQDYDAEKLKREAVEKFSMKNVGKTIAQQYEKILGKNSE"
     gene            complement(3210..4199)
                     /locus_tag="PLZ54_08040"
     CDS             complement(3210..4199)
                     /locus_tag="PLZ54_08040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOJ66907.1"
                     /translation="MNSSLSVIVPCFNEEEVISESYHRLKKVLQELPLASQIIFVNDG
                     SIDGTEKILSEIAQADKTVKVISFSRNFGHQNAVTAGLNHCLSDLAVVIDADLQDPPE
                     LIPAMIETLEKNNAQVVYCVRKHRKGEGFFKKISAKLFYRFLNFFSEVRLPVDTGDFR
                     LVDKNVIREFNKLHEKGKYIRGLIVWLGFKQVPFYYEREPRFAGETKYPFSKMMKLAT
                     TSLLYFSKKPLKIATYLGFFTVIIALLLALWAIFGKFFGFTNADPGWTSLMVAVVFFG
                     GVQLLTVGILGAYIGNLFDEIKDRPEYVIKDMMNFETGNSTQIADEQTGLQNG"
     gene            complement(4196..4591)
                     /locus_tag="PLZ54_08045"
     CDS             complement(4196..4591)
                     /locus_tag="PLZ54_08045"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOJ66908.1"
                     /translation="MNYPKTLVQFLKFGIVGISNTLLTAVTIWLLLRVFHSSNYLANI
                     VGYVVGLVNSFIWNRRWTFENHAQIGATIFKFIVTFAVSYLLQLGFLWLLLHQTEIDD
                     YFCQLLSIVVYTVVNFFMNKYYTFKTEIK"
     gene            complement(4588..>5268)
                     /locus_tag="PLZ54_08050"
     CDS             complement(4588..>5268)
                     /locus_tag="PLZ54_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66909.1"
                     /translation="EFLLRSVENGRIIVWVFSLFAFYWIAKKKVPISNELKMLICCFL
                     MLFSIYFLFVFITRMPFSARYFMPHFFLLTLITLSVFSFRLPETKWKATVLVFLLFEL
                     TGHLWIYPEKMAKSWDCTLAHLPYYELRKQCFDYIDRQNMDYERISAGFCLYGDRGFT
                     ELNQAGKKVGTQLNNQYFIYSNISNLPDEWIDELHNPAHWKPLRTFEKWPVQITVFEN
                     ISFKQTEK"
BASE COUNT         1728 a         1048 c          877 g         1615 t
ORIGIN      
        1 aaccaatttt caattcgatt tttctacctg atgatgctaa tggacaattt gggattattt
       61 tcttaactta ctgtaataca aatatatgaa ttttcaaaaa caacattttt aaaaactttc
      121 gtacggcagt gaaattttgt atcctttttt atctcaatgt cttaaaaaaa ctaattctct
      181 ttgagtaact tttcggagaa gacttattta tttatttatt ctacaacaac aatttacagg
      241 cagccagtaa agtaagcact tgcgctttct ccgtaatgtt tcctttctta taaaggaatt
      301 gacaatcgtc gaataaatcg ctgcaggatg agagcaaatg aatattttcc aaaaaatatt
      361 gatccagcgt ttgccgaagc gacaagtttt tatcgtacca aagctgctcg ggattcatgt
      421 tgtttttcca catggtttga agaggcatac gtttcaccaa agcacgacga tagcgataat
      481 aacaccgcaa tatactgttg ttggtcggtt ttccacctat ggattcccat gtaaaaccgg
      541 taaattcggg atgtttgatt tttatccaat caatatagat ttgttcttta tatcggtatt
      601 ttgcgggtat tgaaagagca taagacaaaa aatcgggatt taaaaaagca gcatccgatt
      661 cgccacacaa atcgaaatac aaaaaaccgt tattcatgcc ggaaaaacct ctgttgtaaa
      721 atttatacat ttcaatgtca gaataatttt ggatgtattt ctgaaaaact ggagcatatt
      781 tattcaataa ttgggtagaa tagacaccat cagaaatttg tggtttggtt ttatgatccg
      841 aaatcagata acttcccaac agagcatctc ccagcatacc ggtatgaatt atgcccgtat
      901 tggtaattct gtgccgtttt atggcatcaa acactgcact tgctccggta taaatggtta
      961 gtccatcgtt caccagcacc acctcttcaa gaggtttcag actttcggct tcaatagaaa
     1021 aagtttccaa cggaacttga tattgttcag aaatttgccg cgcaattatt tcatcagcat
     1081 agccctttgc agaaaaattt aaaaacatct ggtttgaaaa gcccaactgc aaagccgtga
     1141 gtgccaccat tcgcgaatcg aggcctccgc tcagagtagt cagagaaatc aattgatgtt
     1201 ccttgtcaag agaaaattcg ttatcaatcg acttcttaaa aagaaaatcg agttgttcga
     1261 caatatcgga ttttgcatcg tgattttctt gaatatcctg aagatgaaaa taaaaatctg
     1321 tctttaagtt gaaattgcct atttgcaaaa attctccggc ccgcaattgc ttcacttctt
     1381 ccaacaaagt taagttttcg tgcataaatc cacttgtgag taaaaaaacg gcggcttcct
     1441 tgtctaaagt tctttttatc ttttcggttt cgagagtttt gctcagtgtg tacaaatcgg
     1501 tacctatcac aatggtattt tgatcggcaa aataaaaaag ttttttcgat gccgtgtgat
     1561 tattgaaagc gatacatgtt ttctcttttt tgaaatatac aaatccgaca aaatgccccc
     1621 ggaaattttt aaaaaaggac ttttctttta ctattttcct gagcaacgca tcataatttg
     1681 ctgccttatg ttccaagcaa agggctttca gattagtaat gacgccatcg gtaagaataa
     1741 aaaagtcttc gttgtccagc cacaactttt ccgaaataaa ctttgtccct gtaaattgtt
     1801 ccaccacaat atgttcgtca gaaaaagttc gaagcgcaag tttttgctga aagtcaaaag
     1861 gcttcttcca acagaaatgg ggttgctgct ttatggcttt ttttgaaata agaacattaa
     1921 atccgtgcat agaaccgttt ctgaaataat tgttacaaac tgagttggca aaaaaatgga
     1981 attacaatga aaaaaactta atcaggcaac aaacttgatg attgaagaat ttattattcg
     2041 gaatttttcc ctaaaatttt ttcgtactgt tgagcaatcg tttttcctac gtttttcata
     2101 ctgaattttt cgacagcttc gcgtttcaat ttttcggcgt cgtaatcctg aaaatgatcg
     2161 agcatgaaat ttaaactttc aaaaagcata ttttcgtccc ttgcgggaat caatatcccg
     2221 tttgtttcat taatatgctc ggcaattcct ccaacatccg tactgataac gggcttaccg
     2281 caaaccaaac tttcagaaat aacaacaggg gaattttcgt aatttgaaaa caatacaaaa
     2341 aaatcggcat ttttcatgta ttcggcaact tcagcaggat ttttttcacc cacaaaatga
     2401 atcatgtctt ccggaaaatt taatttgacg gaataatcta ccacttcctt aaaatcaacg
     2461 cctgttccga taaaaatcat tttgaaatca tttcgattta cagacaaagc tttcaacgaa
     2521 ttcaacaaac cttttacatt tttttgctga tcatcaaaac aggaaacatg aagaataatt
     2581 ttttttgttt ccgatacttt ggaaatatct gtcaagaaaa catcatcaac cgcattgttt
     2641 acaacaaaat aatttttatt ccataacttg ttggaaatca ttgcattttt cagattttcc
     2701 gacacaggga aaactgcagc agcatgtttg accacaaatt cggtaatttt ttttctcaat
     2761 aatccgttat aactttttct taagggtaaa tacctcgtcc aatgctccgt aattacatag
     2821 ggaatgccgg taaacaaatg aattccgtaa gccatcacga cggtacgcgt aagtatattg
     2881 gcatgaacaa tgtcgggacg gaaaccggat ttttgcaaca tacgccaacc tttgagatat
     2941 gcccgtaaaa aatttatctg ttttaaaagt ttgctaaaca cgcctgattt ggaagtcgga
     3001 aaataaacaa tggtttcttt cacttttcca ttctggcgaa tttcggtttc aaaatgccga
     3061 atatcggacg aaggatgcac ataaagtact tgaacatcac aatacaaact cacagcttcg
     3121 gcatgttttc taacaaatag cccaaacatt tcgtcgtatc gattgggata ccatgccgat
     3181 atgaaaagcg tttttatcat tttttcaaat tatccgtttt gtaaacctgt ttgttcatca
     3241 gcgatttgtg tactgtttcc tgtctcaaaa ttcatcatat ccttgataac atattcaggc
     3301 cgatctttaa tttcgtcaaa caaattaccg atataagcgc caagaatccc gaccgtcagt
     3361 aattgaacac ctccaaaaaa taccaccgca accatcagtg acgtccaacc cggatcggca
     3421 ttggtgaaac cgaagaattt tccgaaaatt gcccataatg ccagcagcaa agcaataatt
     3481 accgtgaaaa aacccaaata ggttgcaatt ttcaaaggct ttttagaaaa ataaagcaag
     3541 gaagtagttg ccagcttcat cattttggaa aaaggatatt tggtttcacc cgcaaaacgg
     3601 ggttctcgct cgtagtaaaa cggcacctgt ttaaagccca accaaacgat cagtccacga
     3661 atatatttcc ctttttcgtg caacttattg aattctctga tcacattttt gtccaccagt
     3721 ctgaaatcgc ccgtatcgac cggaagtctg acttcggaaa aaaagttcag aaaacgatag
     3781 aacaattttg cagagatttt tttaaaaaaa ccttctccct ttcgatgttt tcttacacaa
     3841 taaaccactt gggcattatt tttctccagc gtctcaatca tggccggaat caattcggga
     3901 ggatcctgca aatcggcatc aataactacg gccaaatcgc tcaaacaatg atttaaacct
     3961 gccgtaacgg cattttgatg accgaaattt ctggaaaaac tgatgacttt aaccgttttg
     4021 tcggcttggg caatttccga taaaattttt tccgttccat ctatacttcc atcattcaca
     4081 aaaattattt gtgaagccaa aggcagttcc tgtaaaacct ttttcaaccg atgatatgat
     4141 tcgcttatta cctcttcctc gttgaaacac ggaacaataa cactcaagga tgaattcatt
     4201 ttatttcggt tttaaaagta taatatttgt tcatgaaaaa attgacaacc gtataaacca
     4261 caatcgacaa taattgacaa aaataatcat cgatttcggt ttgatgcagc aaaagccata
     4321 aaaatcccaa ttgaagcaaa taggaaacag caaatgtaac aataaatttg aaaatggttg
     4381 cgccaatttg cgcatgattt tcaaaagtcc agcgtcgatt ccaaataaag ctgtttacca
     4441 acccaaccac gtaaccaaca atattggcca aataattgga agaatggaaa acccgcaaca
     4501 acagccatat cgttactgcg gtaagcaacg tattggaaat tccgactatt ccgaatttca
     4561 gaaactgaac caatgttttg ggataattca tttttctgtt tgtttgaaag aaatattttc
     4621 aaaaacagta atttgaaccg gccatttttc aaaagtacgt aaaggtttcc aatgagccgg
     4681 attatgcaat tcatctatcc attcatcggg caaattggaa atattggaat aaataaaata
     4741 ctgattgttg agctgtgtac cgactttttt ccctgcctga ttcagttctg taaagcctct
     4801 gtcgccatac agacaaaaac ccgccgaaat gcgctcataa tccatgtttt gccggtcgat
     4861 ataatcaaaa cactgctttc ttaattcgta atagggcaaa tgtgccaacg tacaatccca
     4921 cgattttgcc attttttcgg gataaatcca taaatggccg gtcaattcaa acaaaagaaa
     4981 aacaagtact gttgctttcc atttggtttc tggcaacctg aaagaaaata ccgaaagggt
     5041 aatcagcgtc aacaggaaaa aatgaggcat gaaatatctt gccgaaaaag gcattctggt
     5101 gataaaaacg aacagaaagt agatactgaa gagcatcaga aaacagcaaa tcaacatttt
     5161 caattcgttg gaaattggaa ctttcttttt ggcaatccaa taaaaagcga atagcgaaaa
     5221 cacccacacg atgatgcgcc cgttttcaac cgaacgaagc aaaaattc
//
LOCUS       DAOSCH010000124         5258 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_9907_length_5258_cov_6.002499, whole genome shotgun sequence.
ACCESSION   DAOSCH010000124 DAOSCH010000000
VERSION     DAOSCH010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9907_length_5258_cov_6.002499"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..1741)
                     /locus_tag="PLZ54_08055"
     CDS             complement(308..1741)
                     /locus_tag="PLZ54_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOJ66910.1"
                     /translation="MKKIALFTLLCLSINLFSEVITWQGPVGFSSTKYYEVKVNGIPV
                     PVYDTPIASYAIFDFTGKVDVEVSTMFDVRWVDIRPLRTQIKPHYTSDNSFRFNLDKP
                     QNLSLELNGRIRQQPLFIFAGKPETDIPSKSDPNVIWFEGGKLYKNVNLELNDNQTVY
                     IEGGAVVQGIIIANGKKNIKICGKGILDGTYNRQTAGNDNRFIKLVDCENIDINDITL
                     HNGTTWQVHLRHCNNSRINGIRIVSENGGDDGMDIVRCTNVVIDGVFAHTKDDCIAIK
                     SHGDYPPHRITDNILVKNCVFWNSIWGNAIEIGFELYSDEVKNIRFENIDIIHVEDGA
                     AMSIHNAGKAHVHNVVFDNIRIEDTRQKLFDVAIFFSQWGPDGIRDPEYITKYYLHGA
                     WDGVQKAPAGKEAYHSSFRGKVSNITFKNIHVTGGLLPFSVFHGFDAEKNVSNITIEN
                     LRYIGKKLKTTDDAKIRQQNTVNLKIK"
     gene            complement(2018..2296)
                     /locus_tag="PLZ54_08060"
     CDS             complement(2018..2296)
                     /locus_tag="PLZ54_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66911.1"
                     /translation="MMQQLKVDKNHFSAWNKNYATGFSALPAGLAYDNVLGRKGDWAI
                     FWASNSSGEHYAWSVEMDNFYPVLSGNSHLMLTNTYLKKCFFSSLRKE"
     gene            complement(2293..2487)
                     /locus_tag="PLZ54_08065"
                     /pseudo
     CDS             complement(2293..2487)
                     /locus_tag="PLZ54_08065"
                     /inference="COORDINATES: protein motif:HMM:NF021143.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
     gene            3049..3300
                     /locus_tag="PLZ54_08070"
     CDS             3049..3300
                     /locus_tag="PLZ54_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66912.1"
                     /translation="MDTDDLSGEAYWAILGEAEKLTENLTLRYGLLSYECKNETEYLE
                     KAKKLSYQIMKLNDNALYDLFFGDPPEKKDLQATLKKNY"
     gene            3345..3845
                     /locus_tag="PLZ54_08075"
     CDS             3345..3845
                     /locus_tag="PLZ54_08075"
                     /inference="COORDINATES: protein motif:HMM:NF040080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HOJ66913.1"
                     /translation="MTSKEEIKQKEQQLLEMTKAFCSKKLNGVYAQLCEKLIKKMGRK
                     RDVPFQRGKLEIWAAAVIQAIGSINFLFDKSFEPYLSTDEINQYFGTKNSTVSSKARE
                     IKEMFRLQYYDPEFSTKSMTDSNPFNNMVMVNGMIVPLDILPENLQQEVKAARARGED
                     IEFYTE"
     gene            4200..>5258
                     /locus_tag="PLZ54_08080"
     CDS             4200..>5258
                     /locus_tag="PLZ54_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66914.1"
                     /translation="MATKGGSIMGKNIQVLSVLIVVLLLIDGCTKKPANYYRKVNIGS
                     KYEIIGRYPVKDNMASKINCYHFIYNEKGKLAKVEYLKGGKLQNDSYFGVARIIIEHS
                     EGYEKRIYQDAKGKPTVSSDSVYSIRLKLNESGYPISIFNYDEEGKLVEDKDGVAQYL
                     LTLDEKGRRIKSIRIDKKGDRIIDVNGFYELIWKYDKKGNTIEQSNHGKDGQLLEGKN
                     NIAIIRYKYDEQGNKVEERYFGSDEQLKENKDWGFAIIRRKYDEQGNVVEERFFGTDE
                     QLKEDNHWGAAIIRWKYDEQGNVVEERFFGTDEQLKEDKDCGVAIIRYKYDKQGNRVE
                     VRFFGTDEQLKEDKDLGVA"
BASE COUNT         1752 a          861 c          975 g         1670 t
ORIGIN      
        1 acaaaaatat gaaattctca ggcattttta atttctaaag tttcggatag tagtgccttt
       61 gacaataatt cccggctgtc cttcttatta attaattctc tcggcgataa aaataaatct
      121 aattgtactt cactttctgt tttcctattt ctctctttct ttatcatatt tcattctatt
      181 aagatactga aatataacaa tatatgcaaa ttttcaaaga acttttttga atattttttt
      241 actctttttt atctcaatgt ctttaaaaaa ctaattctct atgagtaact ttttctgagg
      301 agactcatta tttaattttt aaattaacag tattttgttg tctgatttta gcgtcatcgg
      361 tagttttgag ttttttacct atataacgta aattttcaat tgtgatgttt gaaacattct
      421 tttcggcatc aaatccatga aaaacagaga aaggtaacag cccgcccgtt acatgaatgt
      481 ttttgaaagt aatgttacta acctttccac ggaaactgct gtgataagct tccttgcctg
      541 caggagcttt ttgaacccca tcccaggctc cgtgcaaata gtatttggtt atatattcag
      601 ggtcgcgaat accatcaggt ccccactgcg agaagaaaat ggcaacatca aacaatttct
      661 gacgcgtatc ttctatacgg atattatcga aaaccacatt atgaacatgt gccttaccgg
      721 cattatgaat gctcatggca gctccatctt ccacgtgtat aatgtcgatg ttttcaaaac
      781 ggatattttt aacttcatcg gaatacaatt caaaacctat ttcgatggca tttccccaaa
      841 ttgagttcca aaacacacaa tttttcacta aaatattatc ggtaatgcga tgaggcggat
      901 aatcgccatg cgattttatg gcaatacaat cgtctttagt atgtgcaaat acgccatcaa
      961 ttactacatt tgtacagcgt acaatatcca tcccgtcgtc gccaccgttt tcactcacga
     1021 tacgtattcc attgattcga ctattgttac aatgccgcag atgtacttgc catgtagttc
     1081 cattatggag agttatatcg ttaatatcaa tattttcaca atcgactaac tttataaaac
     1141 gattgtcgtt tccggcggtt tggcgattat atgttccatc taaaattcct ttgccgcaga
     1201 ttttaatatt ttttttgcca ttggcaataa taataccctg taccacagcg cccccttcga
     1261 tataaacggt ttgattatcg ttcaattcaa gatttacatt tttatagagt tttccacctt
     1321 cgaaccatat tacattcgga tctgactttg aagggatatc tgtttcgggc tttcctgcaa
     1381 aaataaataa tggttgctga cgaatacgcc cgtttagctc aaggctaaga ttttgtggct
     1441 tatccaggtt gaatcgaaag gagttatcac tcgtgtaatg gggtttaatt tgtgtgcgga
     1501 gaggtcgaat atccacccaa cgaacatcaa acatggtgct tacttcaaca tcaacttttc
     1561 cagtaaaatc gaatatggcg tatgatgcaa ttggcgtatc gtaaacggga acagggattc
     1621 catttacttt tacctcgtag tatttggtag atgaaaaacc aaccggacct tgccatgtga
     1681 tgacttcact aaacaaattg atgcttaagc atagaagtgt gaataatgca atttttttca
     1741 tttttttgta ttgtaagatt tctgatacaa aaataagtca tttgtttttt aaaaaactat
     1801 ttttttttca catatcatga tacttttacg actatttttt ttatttattt gtgttgcttt
     1861 ttgttgtttt agtatcataa ttttcgggtc tgattactgt atatttcctc tgcgaatttc
     1921 agtctttttt ctatagaagc ttataatagg cactaaagga tggttcaagg acaaagagcc
     1981 atttagcaga tgagataaga cttttgaaaa ttatttctta ctctttacgc aacgaactga
     2041 aaaagcattt tttcagatac gtatttgtca acatcaaatg ggaatttcca ctcaaaaccg
     2101 gataaaaatt atccatttca acactccatg cataatgttc tcctgaggaa tttgacgccc
     2161 agaatatcgc ccagtcgcct ttacgcccaa gcacattatc gtaagccaat ccggctggaa
     2221 gtgcactaaa acccgtagca taatttttat tccacgcaga aaaatgattt ttgtcaactt
     2281 taagttgttg catcatgtcc agattgtcgt acaatttgaa gagctcttcc cagtctcttt
     2341 tgtagctaca tgccagcagt caggacaaat tccctgattg gacaaacagt cgtacatcat
     2401 tacttcgtcg aatgtataaa gtccaccata aacgatacag tttgttgaat cgttattgta
     2461 gcaggttttt tccacaatgc tgttattttg ttgcttggtg ttttgaacag acctacattc
     2521 atgtttttag tcatccacga agttccgttt attgtgactg taggatattt aattcaagta
     2581 cgagcatata agtaatcgtg tagtcttttg taatctgggc tttagtatat gactaaaatt
     2641 gagacattta ttatctgtaa aacagtacat ataagtcaag ttctcgggtt gaccttcact
     2701 ggtgaaccga aaaatcagtg aagttggcgt taaaaaatct tctctgtttg agcgacggag
     2761 gagcgggtgt agaagatttt agccaacaaa cgtccatttt tcttgtagaa actttgcatg
     2821 caacgtctcg ctgaaaacaa taaaaaagaa catgaagttt agaacaaagt cccgttatta
     2881 aatatctgaa aataaaaaac ataaatgttt ctacaatttt tgtgtcatgt actaagaaaa
     2941 atactttatt tttgtttgaa aattaaatgt attttagttc tcataagcta atattttttg
     3001 aaaaaaagcg tgtataaata gttttaacga gtaaaataaa tcttttttat ggatacagat
     3061 gatttatcgg gagaagccta ttgggctatt ttaggagaag cagaaaaact gactgaaaat
     3121 ttaactttgc gatatggctt actgtcttat gaatgcaaaa atgaaaccga gtaccttgaa
     3181 aaggcaaaaa aattatctta tcaaataatg aaactcaacg ataatgcatt gtatgatctc
     3241 ttttttgggg atccgccgga aaaaaaagat ttgcaagcca ccttaaaaaa aaattattga
     3301 caacatagaa aaagttcaaa aaacacccat tcaaaatcga cattatgact tctaaagaag
     3361 aaattaaaca aaaagagcag caattgctgg aaatgacgaa agcattttgt tcgaaaaaat
     3421 taaatggcgt gtacgcacaa ctctgcgaaa aactgattaa gaaaatggga cgaaaaagag
     3481 atgtcccctt tcaaagagga aaactcgaaa tttgggcagc cgccgtgatt caggccatcg
     3541 gttccatcaa ttttctgttc gacaagtcgt ttgaacctta cctgtcaacg gatgaaatca
     3601 atcaatactt tggaacgaaa aattcaacgg tatccagcaa agctcgcgaa ataaaagaga
     3661 tgttccgctt gcaatattat gatccggaat tttctacaaa aagcatgaca gactccaatc
     3721 cgttcaacaa tatggttatg gttaatggta tgattgtgcc attggatata ttgcccgaga
     3781 acctgcagca agaagtcaaa gcagcccgag ccagaggcga agatattgaa ttttatacgg
     3841 aataaaaagg atagggattc atagttttat cggcaggtgt tggggtgtac aataagcgaa
     3901 tgttttcctt ttttactttc caatatatca atcaattgat aacttataaa tattttagtg
     3961 atttgttttg aattataaaa ttgttctgta aatttgtgaa ataaactttt gtttcataaa
     4021 aaaacatata cgcacgcgta tcacaaaaaa gtttacagaa aataaaatcg ccgtttggtg
     4081 atttataaga atttgataaa gttgttatta aaaatagcac actattttat atcgccgata
     4141 cacccaaaaa tggaaataga gagaaataat gtagcgccta taaacaagtt atacgccata
     4201 tggcaacgaa aggaggaagt attatgggga aaaatattca ggtcttgtct gttctgattg
     4261 tagtattgct tttaatagat ggttgcacta agaaacctgc aaactattat cggaaagtta
     4321 atattggtag caaatatgaa ataataggga ggtacccagt taaggataat atggcaagca
     4381 aaattaattg ctatcatttt atttacaatg aaaaaggcaa acttgcaaag gttgaatacc
     4441 tgaaaggtgg caaactacag aatgattcct attttggtgt agcacgaatt ataattgagc
     4501 attcggaggg atatgaaaaa agaatttatc aagatgccaa aggtaagcca acggttagtt
     4561 ccgacagcgt atattctatc cgtttaaaat tgaatgaatc tggttatcca atatcaattt
     4621 tcaattatga tgaagaaggg aaattggttg aagataaaga cggtgttgcc cagtacttac
     4681 taacgcttga tgaaaaaggg cgaagaataa aatccattag aattgacaaa aaaggtgata
     4741 gaattataga tgttaatgga ttttatgaat tgatatggaa atacgataaa aaaggaaata
     4801 cgattgagca gtcaaatcat gggaaagatg gacaattact tgaaggaaaa aataatattg
     4861 ccataatccg ttacaaatat gacgagcaag ggaacaaagt agaggaaaga tattttggct
     4921 ctgatgagca gttgaaggaa aataaagatt ggggatttgc cataatccgc cggaaatatg
     4981 acgagcaagg gaatgtggta gaggaaagat tttttggcac cgatgaacag ttgaaggaag
     5041 ataaccattg gggagctgca ataatccgct ggaaatatga cgagcaaggg aatgtggtag
     5101 aggaaagatt ttttggcact gatgaacagt tgaaggagga taaagattgt ggagttgcca
     5161 taatccgcta caaatatgac aagcaaggga acagggtaga ggtaagattt tttggcactg
     5221 atgaacagtt gaaggaggat aaagatttgg gagttgcc
//
LOCUS       DAOSCH010000125         5230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_9978_length_5230_cov_6.286184, whole genome shotgun sequence.
ACCESSION   DAOSCH010000125 DAOSCH010000000
VERSION     DAOSCH010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9978_length_5230_cov_6.286184"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            333..1520
                     /locus_tag="PLZ54_08085"
     CDS             333..1520
                     /locus_tag="PLZ54_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOJ66915.1"
                     /translation="MSLLSTVMGQYDEPQKVKSLGIYPYFRPIQSEQDTVVTINGKPV
                     LMFGSNSYLGLTNHPKLKEAAIRAIEKYGTGCAGSRFLNGTLDIHIELENRLAKFVHK
                     DAALVYSTGFSVNSGVIPCLTGRNDYLIFDERDHASIIEGKRLSFSKQFKFRHNDMDS
                     LEDVLKRCDYDKIKVIVVDGVFSMEGDIAKLPEIVELSKKYNASIYVDEAHTLGVFGK
                     TGAGICEHYGHTNDVDLIMGTFSKSLGTIGGFIASDNNIINYLKHNSRTLIFSASITP
                     ASAGCVLAALDVLEEETWRKEALWKNTARAKDGFMKAGFEIGHTESPIIPLYVRDNLK
                     TFQLTSLLMEEGVFVNPVVSPAVPSEDTLIRYSLMATHTFEQIDESIEKITKVARKLG
                     IIE"
     gene            complement(1722..2147)
                     /gene="ssb"
                     /locus_tag="PLZ54_08090"
     CDS             complement(1722..2147)
                     /gene="ssb"
                     /locus_tag="PLZ54_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005851334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOJ66916.1"
                     /translation="MSVNKVILVGNVGKDPEVRYLEKNVAVARFPLATTERGYTMQNG
                     TQVPDRTEWHNIVAWRGLAEIVEKYVKKGSQLYIEGKIQSRSWEKDGIKRYTTEIYAE
                     NIQLLGKRSDTSEEPAAASTPPAETFQEGADIVEDDLPF"
     gene            complement(2292..2918)
                     /locus_tag="PLZ54_08095"
     CDS             complement(2292..2918)
                     /locus_tag="PLZ54_08095"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOJ66917.1"
                     /translation="MKKRIALSGCLLFFLAMNLFSQFSLQGGLNRASEIYVGSSASDA
                     NLPALKNLTGYNIGMMYQTPQKGFGFATGIALSQKGSIVNSDSLTDVYRQFNFLEIPL
                     HLRYCLDFGNMGLYGFGGVYGAYALSGQKISETPETQNNENFTYSDFNCRVDYGYTFG
                     AGIQFFKKIQIQATWNQGWKNLTNYLNGEKEPFSTKNKIFAVNLSYLF"
     gene            complement(2974..4791)
                     /gene="typA"
                     /locus_tag="PLZ54_08100"
     CDS             complement(2974..4791)
                     /gene="typA"
                     /locus_tag="PLZ54_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOJ66918.1"
                     /translation="MQKIRNVAIIAHVDHGKTTLVDKMLLAGHLFRNNEKVGELIMDN
                     NELERERGITILAKNVSINYNGYKINIIDTPGHADFGGEVERVLNMADGVLLLVDAFE
                     GPMPQTRFVLQKAIQMGLIPIVVVNKVDKPNCRPDEVQEAVFDLMYHLDANEEQLNFH
                     TIFGSAKQNWMSDDWKKPTDNILPLLDAIIKYIPAPPYLEGSPQMLITSMDYSPYVGR
                     IAVGRLHRGTLREGMEVSLVSRDGKVTKSRIKELHTFTGLGRTRTEEVKSGDICALVG
                     IEGFEIGDSIADALQPEPLKPIAVDEPTMSMVFTINNSPFYGKEGKFVTSRHIHERLM
                     RELEKDVALRVEKNPDSDIWHVYGRGVLHLSILIETMRREGYELQVGQPQVIFKEING
                     VRCEPVEQLSINLPEEYAGKIIEMVAARKGEMLSMEIVNDRVQLEFQIPSRGIIGLRN
                     NVLTASTGEAVMSHRFLEYQPYKGEIEKRNNGSLIAMESGTAFAYAIDKLQDRGRFFI
                     FPQEEVYAGQVVGESAKEGDIVVNVTKSKKLTNMRASGSDDKIKLTPPVVFSLEEALE
                     YIKEDEYVEVTPTKIRLRKIYLDENERKRMSKKSNVLSE"
BASE COUNT         1548 a         1014 c         1001 g         1667 t
ORIGIN      
        1 gactaaaaaa aataatccta atatcatatt gttctaatgg acaatttggg tttattttat
       61 aacctttgat ttgctgttaa gataatcaca ttagaccttt caaaaaaaat tcagcatact
      121 tttttaacta tgaaacccac aagtattgca tacacaaaca cgaatgaata taatatttaa
      181 tattcagaca tgtgggttcc atcagacctt aaaaaaaaaa attatattct gatgaatatg
      241 aatctaaaat tttctgtttt taaaaagcat taattattaa tcaaaaagat gtatctttgt
      301 gccgttttta ataaaaatat aaactataac ttatgagttt gttaagtaca gtaatggggc
      361 agtatgatga acctcagaaa gtaaaatcat tgggcattta tccttatttt cgtcctattc
      421 agtcggaaca ggatacagtt gttactataa acgggaaacc tgtattaatg tttggatcca
      481 atagttatct gggccttacc aatcatccga aactgaaaga agccgctatc agagcaatag
      541 aaaaatacgg cacaggttgt gcaggttcgc ggtttctgaa tggaacactt gatattcata
      601 tcgaattgga aaaccgtttg gcaaaatttg ttcataagga tgctgctttg gtatattcca
      661 ctggtttttc ggtaaattcg ggtgtaattc catgtttgac gggacgaaac gattatctga
      721 tttttgacga aagagatcac gcttctatta ttgagggaaa gcgtctttcg ttttcaaaac
      781 agtttaaatt ccgtcacaac gacatggatt cgctggaaga tgtgctaaaa cgttgcgatt
      841 atgacaaaat aaaggtgatt gtagtggatg gcgttttcag tatggaaggc gacattgcca
      901 aattgcctga gattgtggaa ttatccaaaa aatacaatgc ttccatttat gttgatgaag
      961 ctcatactct gggcgttttc ggaaaaacag gagcaggaat ttgcgagcat tacgggcata
     1021 ccaatgatgt tgatctgatt atgggaactt tcagcaaatc gttgggaacc attggcggtt
     1081 ttatcgcttc cgacaacaat attatcaatt atttgaaaca caattcgaga acattgatat
     1141 tcagcgcttc cattacacct gcttctgcag gttgcgtgct ggcagctttg gatgtattgg
     1201 aagaagaaac gtggagaaaa gaagcgttgt ggaaaaacac agcccgcgcg aaagatggat
     1261 ttatgaaggc cggttttgaa attgggcaca ctgaatcacc catcattcct ttatatgtgc
     1321 gggataatct taagactttt caacttacca gtttgctgat ggaagaagga gtttttgtta
     1381 atcctgttgt ttcgccggct gttccctccg aagatacact gatcagatat tcgttgatgg
     1441 ctacccatac gtttgaacag attgacgaat caatagaaaa aattacaaaa gttgcccgca
     1501 agttgggaat tatagaataa ttacacgaaa gttttttcat tcaattgtca acagaatgtg
     1561 tcaaattatt gatacattct gttttgcttt taaagaaata gactgacgat aaccatttat
     1621 atattttgtg caagttttaa aatatggtag gataaaaaca ggtagaacaa aaaaagcaca
     1681 caacaatagc gtcatgtgct ttggagaaaa taagaatttt ttcagaaagg caaatcgtct
     1741 tctactatat cagcaccctc ctgaaaggtt tctgcagggg gagtagaagc tgcagcaggt
     1801 tcttcagatg tgtccgaacg ttttcctaac agttggatat tttcagcata aatttccgta
     1861 gtgtaacgtt taattccatc tttctcccat gagcgacttt gaatttttcc ctcgatgtaa
     1921 agctgacttc ctttttttac gtatttttct acaatttctg ccagccccct ccaagctaca
     1981 atattgtgcc attcagttcg atcgggaact tgcgttccgt tttgcatggt ataaccccgt
     2041 tcagtggtag ccaaaggaaa acgggctact gcaacatttt tttccaagta acgcacttcg
     2101 ggatcttttc ctacattccc tactaaaata actttattaa cagacataat ttcaatgatt
     2161 aaaatggttt gaattgagtt gaactgcccg taaaaataca ttttttttta tacgtatcaa
     2221 atttttttca aaaattttga aaaaaatgag cttgattttt gtcgtcgatg attgtaaaat
     2281 agaatggttg tttaaaacaa atatgacaaa ttgacggcaa atattttgtt tttagtagaa
     2341 aaaggttcct tctccccatt caaataattg gttagatttt tccatccctg attccaagta
     2401 gcctgaatct gaattttttt gaagaattga attccggctc caaatgtgta gccatagtcg
     2461 actcgacaat taaaatcgga ataagtaaaa ttttcgttgt tttgggtttc aggtgtttca
     2521 gaaatttttt gtccactcaa ggcataggcg ccgtaaactc ctccaaatcc atacagcccc
     2581 atatttccaa aatccagaca atagcgcaaa tgcagaggga tttccagaaa attgaattgg
     2641 cggtaaacat ctgtcaagct gtcggaattt acaatactgc ctttctgaga aagggcaata
     2701 cctgtggcaa aaccaaagcc tttttgtgga gtttggtaca tcatcccaat gttgtatccc
     2761 gtcaggtttt ttaaagccgg caggttggca tcagaagcac tactgccgac gtaaatttct
     2821 gaagctctgt tgaggccgcc ttgtagggag aattgcgaaa ataaattcat tgcaaggaaa
     2881 aataacaaac atcccgacaa ggctattctt tttttcataa attcttgtgt accgcttttt
     2941 tttaatggaa tcgttttgtt ccgaaataaa ttcttattca ctgaggacat tcgacttttt
     3001 cgacatgcgt ttacgttcgt tttcatcaag atagattttc cttaagcgaa ttttggttgg
     3061 agtaacttcc acatattcgt cttctttgat atattccaaa gcttcttcga gactgaaaac
     3121 taccggtggt gtaagtttaa ttttgtcatc agaacctgat gcccgcatgt tggttaattt
     3181 tttcgacttg gtaacattca ccacaatgtc gccttcttta gcgctttcac ccacaacctg
     3241 accggcataa acttcctcct ggggaaaaat gaaaaatctg cctctgtcct gtaatttgtc
     3301 gatggcataa gcaaatgccg tgccactttc cattgcaata agcgatccat tgtttctttt
     3361 ttcgatttca cccttgtaag gttggtattc caaaaatcgg tgcgacatga cggcttctcc
     3421 tgttgaagct gttagtacat tattccttaa tccgataata cctcgtgaag gaatttgaaa
     3481 ctccagctgt acacggtcgt ttactatttc catgctcagc atttcgcctt tgcgggctgc
     3541 aaccatctca atgatttttc cggcatattc ttcagggaga ttgatgctca actgttcgac
     3601 cggttcgcaa cgaacgccgt ttatttcttt gaaaattacc tgtggctgtc ctacctgaag
     3661 ttcgtaacct tctctgcgca tggtttctat cagaatggaa agatgcaaaa cacctcttcc
     3721 atagacgtgc caaatatccg agtctggatt tttctccacg cgtaaagcga cgtctttttc
     3781 gagttctcgc atcaatcgtt catgaatgtg tcgtgaagta acgaattttc cttcctttcc
     3841 gtaaaaaggc gaattattga tggtgaaaac catactcatg gtaggctcat ccactgcaat
     3901 gggtttcaac ggttccggct ggagtgcgtc ggcaatggaa tcgccaattt caaaaccctc
     3961 tattcccacc agcgcacaaa tatcacccga tttcacttct tccgtgcgcg ttcttccaag
     4021 tccggtaaaa gtatgcaatt ccttaatgcg ggattttgta accttgccat cacgacttac
     4081 caggcttact tccattcctt cgcgaagtgt tccacgatga agtctgccaa cggcaattct
     4141 tcccacatac ggagaataat ccatcgaagt gataagcatt tggggagagc cttccaaata
     4201 aggcggagca ggaatatact tgataatggc atccagcaag ggtaaaatat tgtcggttgg
     4261 ttttttccaa tcgtccgaca tccaattctg tttggctgat ccaaaaatag tgtgaaaatt
     4321 caactgttct tcattggcat ccaaatgata cattaagtcg aagacggctt cctgaacttc
     4381 atcgggacgg caattgggct tgtcgacctt attgacgacc acaatgggaa tcagtcccat
     4441 ttgaatggct ttttggagaa caaaacgggt ttgtggcatc ggaccttcaa aagcatctac
     4501 caaaagtaac actccatcag ccatatttaa aaccctttct acttctcctc cgaaatcggc
     4561 gtgtcccggt gtgtctataa tgttgatttt atatccgttg taattgatgg aaacattttt
     4621 agccaatatg gtgattcctc tttcgcgttc gagttcatta ttgtccataa tgagttcacc
     4681 aactttttcg ttgttgcgga aaaggtgtcc tgccagtaac atcttgtcta ccaatgtagt
     4741 tttcccgtga tcgacatggg caatgatggc tacatttcta attttttgca taagttaaag
     4801 agctaatttt ttagcctgca aagatagcca aaaaaattga atttttattg ccagtttttt
     4861 tgaatttagc taaagcaaaa aatatggaac gcatgacaca aatttgaaaa tttcggtttc
     4921 cattgaattg cgttgtatcc cggtagagac gtagcatgct acgtctcaag caaataccca
     4981 aatttaattg caacgaatcg gaatccaaac gcggaaaaaa ttcaaaaatg gtatggtgat
     5041 ttttttcatt gaattgggat atatcaggtt tgagacgttg catgcaacgt ctctacaaaa
     5101 tacctaaatt caattgcaac gaattggaat tcaaaacacg gaaaaaattc agaaattgta
     5161 cagctatttt tttcattgaa ttgcattgta tccgggtaga gacgtagcat gctacgtctc
     5221 atgcaaatac
//
LOCUS       DAOSCH010000126         5220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_9991_length_5220_cov_6.945595, whole genome shotgun sequence.
ACCESSION   DAOSCH010000126 DAOSCH010000000
VERSION     DAOSCH010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9991_length_5220_cov_6.945595"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PLZ54_08105"
     CDS             complement(<1..203)
                     /locus_tag="PLZ54_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ66919.1"
                     /translation="MLKLLQANEINLTEWQQLVENSAVASFFQTTECYQFYTSLSFLK
                     PFLYGVAEDGKLKGIACGYVQAEG"
     gene            complement(196..1110)
                     /locus_tag="PLZ54_08110"
     CDS             complement(196..1110)
                     /locus_tag="PLZ54_08110"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ66920.1"
                     /translation="MKILFVCSKNSGKVASFIVEQAEAVQQAGLEVDFFTIEGKGWKG
                     YLSNRPLLIKKIKEFKPNVIHAHYGLSGLLANTQRKVPVITTFHGSDINDPEVFRFSK
                     WAIRLSAWNIFVSEKNIQAAKVKHKYSLIPCGVNTELFKPVDKHIARKKFGFSTGEKL
                     ILFAGSFDNPVKNSALALQAINFLPEVRLLELKNYSREEVALLMNAVDVCLMTSLSEG
                     SPQFIKEAMACNCPIVSVDVGDVKEQIEDVENCFITEARPEKIAEKIDTIFTNRSKRT
                     NGRERLMKSELEQSVVAKQLIKIYRKYA"
     assembly_gap    1397..1496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1963..2403)
                     /gene="tnpA"
                     /locus_tag="PLZ54_08115"
     CDS             complement(1963..2403)
                     /gene="tnpA"
                     /locus_tag="PLZ54_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOJ66921.1"
                     /translation="MKIEYRNLYTHFIFITQNRLPLIQEQHRERIEKYITGIVSNNQC
                     HLYAIYANPDHIHFLISRDPSMDEQTIATIIEKSSSKFINENNLCVGNFRWQDSCSAF
                     SVSKGDIDKVCKYILNQKEHHKKKTFNEEYDEFMKFYQKTIKPI"
     gene            complement(2629..4038)
                     /locus_tag="PLZ54_08120"
     CDS             complement(2629..4038)
                     /locus_tag="PLZ54_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66922.1"
                     /translation="MLNYFPKYFTQKAMLLYVISLAAVTVVFFSHAMSWIWIFFGLVE
                     VIGFFYFSNELTRQWGKTSPKTFTKRLFTNSLIIRIVWVIFSYFFYQSMTGQPFEFAA
                     ADAQGYHNEAVWLADMIHKGNIQPYFAYINGRFSDMGYPFYLGCLYAVTGKSILFARL
                     LKAVYGAITCVLIYKLTTRNFEESTGRMAGIFAMLMPNLIYYCGVHLKEAEMVLLTVA
                     FIERTDALLRNRKFNFINIFVPTLLGASLFFFRTALGITALFAVLTTLVFAHNKMVRS
                     WKRFIYGLWIGITIIYFAGTSISSEVESLWQNQKENQSTSIQWRAEREGGNRLARYAS
                     GAVFAPLILVIPFPTMVNIEGQENQQTINGGNFVKNILAFFVIFALIWAIRNKVWRNY
                     LLIGSFTIGYLLVLAFSAFAQSERFHQPVLPFILILAAVGISRMTNRSKKYFWWWMGI
                     IFIAIVGWNWFKLAGRGMV"
     gene            complement(4544..>5220)
                     /locus_tag="PLZ54_08125"
     CDS             complement(4544..>5220)
                     /locus_tag="PLZ54_08125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ66923.1"
                     /translation="YQQKYHLKPEKQKTVYNGISFDKLDTVKTDRTKKSTSSELLKLG
                     SVGNFVSGRSQIVICRFLNLLKKENIAFDFVFVGAKSKSEPELFDDCYHYCEENNLLE
                     NVHFLGSRSDVPEILSTLDAFIYSTDHDTFGIAVVEAMAMGIPVFVNDWEVMKEITQN
                     GKFATLYKSRDEYDLLKKFLLFLQNRAIAIRQAREAAAYVRENFSIESHIRQLKQVYE
                     TLDSDD"
BASE COUNT         1542 a          971 c          801 g         1806 t
ORIGIN      
        1 ccttctgcct gcacatatcc gcaagcaatt cctttcagtt ttccatcctc agccacgcca
       61 tacagaaaag gttttaaaaa agataatgaa gtgtaaaatt gataacactc cgttgtttgg
      121 aaaaaggaag caaccgctga attttctacc aattgttgcc attcggttaa atttatttca
      181 tttgcttgca gcagcttaag catacttcct gtaaattttt attaattgtt ttgccacaac
      241 agattgttcc agctcacttt tcattagtct ttctcttcca ttggttcttt tacttctatt
      301 ggtaaaaata gtgtctattt tttcagcgat tttttcagga cgagcttctg taataaaaca
      361 attttctacg tcttcgattt gttcttttac atcacccaca tccacactta ctatgggaca
      421 gttacatgcc attgcttcct tgatgaattg aggcgaacct tcactaaggc tggtcatcag
      481 acaaacatcc actgcattca ttaataaagc tacttcttca cggctgtagt tttttaattc
      541 cagcaaacga acttccggca aaaagttgat agcttgcaaa gccaatgcag aattttttac
      601 cggattatca aagcttccgg caaaaagtat cagcttttcg ccagtcgaaa atccgaattt
      661 tttacgggca atatgtttat caacaggttt aaaaagttcc gtattcacgc cacaaggaat
      721 caaggaatat ttatgtttta cttttgccgc ctgaatattt ttttctgaca caaaaatgtt
      781 ccacgccgat aaacgaatag cccactttga aaatcgaaat acttccggat cgttgatatc
      841 gctgccatga aaagtggtta tcacaggcac ttttcgttgg gtattggcca gtaatcccga
      901 caaaccgtaa tgggcatgaa ttacattcgg tttaaattct tttatttttt ttatcaacaa
      961 aggtcgattg cttaaataac ctttccatcc ctttccttcg atagtgaaga aatccacttc
     1021 caaacctgcc tgctgaactg cttctgcttg ttccacaata aaggatgcaa ctttccccga
     1081 gtttttactg caaacaaata atattttcat gtttatcaat aactctgtta atagattcaa
     1141 gctataagtc aatggtaatt tataaaattt aagaaatttt actactactt aatacttttt
     1201 atcttgtcct tttttgcgtt cggcgtagcg tttcgctacg tcggtttaga cataaagctc
     1261 cagctttttc ataatgattc atcttatgca aaaattattt cgttatcatt cttattttct
     1321 gtttttcatt attatcgaaa taaggtaata aggtctggtt gatatgggct tattattggt
     1381 tactaaggtt cattgtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngtgg
     1501 atattatttc ttgtttcttt ataaaattag ttgtttttgt tgtttaaaaa aacttatttc
     1561 gtttttctta ttaacattag taacaagcgt aatcttaccc gaaaattaac cttattagct
     1621 tattagattg tttttccttt tttgttgttc aaaggtaata aggtctggtt gatatgggct
     1681 tattattggt tactaaggtt cattgtatga taaggttttt cctgttcatg ttgtattagt
     1741 tgatagtgga tattatttct tgtttcttta taaaattagt tgtttttgtt gtttaaaaaa
     1801 ccttatttcg tttttcttat taacattagt aacaagcgta atctcacccg aaatatgacc
     1861 ttattagctt attagatcga attgattgct catcatccat tatttttcaa ttaaccttag
     1921 taacttcaca catcctctct tcttgtaaac cttattacct tattaaattg gtttaattgt
     1981 tttttgatag aatttcataa attcatcgta ctcttcatta aaagttttct tcttatgatg
     2041 ttctttttga ttaagaatat atttacaaac tttatcaata tctccttttg aaacggaaaa
     2101 agctgaacat gagtcttgcc atcgaaaatt tcctacgcat aaattatttt cgtttatgaa
     2161 ttttgatgaa ctcttttcaa taatagtggc aatcgtttgt tcgtccattg acggatctcg
     2221 tgaaattaag aaatgaatat gatcaggatt tgcataaata gcatatagat gacattgatt
     2281 atttgataca attcccgtga tatatttttc aatccgctct cgatgctgct cttggatcag
     2341 aggtaggcgg ttctgagtga taaaaataaa atgagtgtac aaatttctat attctatttt
     2401 catttttctt tctaattgat taaaaataag gtaataaggt taagcaaaat gtgcttatta
     2461 ttggttacta aggtttattg tatgataagg ttttataatt tctttcttca tttcaattaa
     2521 aattggagat tgtactttac cttcgactta gtctagagac tatgctgaac cttaagtttt
     2581 aatttctaca tttcatattc aaaacttctt cctctgtact tattctattt acaccattcc
     2641 tcttccggcc agtttaaacc aattccatcc cactatagct ataaaaataa ttcccatcca
     2701 ccaccagaaa tattttttgg accgattggt catccgactg attcccactg ctgctaatat
     2761 caaaataaaa ggcaacaccg gctgatggaa tcgttccgat tgagcaaaag cactgaatgc
     2821 cagtaccaat aaataaccga ttgtaaatga acctatcaac aaataattgc gccagacctt
     2881 attccttatt gcccaaatca aagcgaagat aacaaaaaat gccaatatat ttttcacgaa
     2941 atttccacca tttattgttt gctggttttc ttgtccttct atattcacca ttgtagggaa
     3001 aggaattacc aatatcagcg gagcaaaaac agcaccggaa gcataacgcg caagacggtt
     3061 gccgccttcc cgttctgccc gccattggat agaggtagac tgattttctt tctgattttg
     3121 ccacaggctt tctacttcag aagatatgct ggtgccggca aaataaataa tggttattcc
     3181 aatccaaagt ccatagataa atcttttcca actgcgaacc attttattgt gggcaaaaac
     3241 cagcgtggtt aataccgcaa ataaagctgt aatccctaaa gcagtacgaa aaaagaaaag
     3301 agaagcacct aagagtgtag gaacaaatat attgataaaa ttaaactttc tgttccgaag
     3361 cagtgcatct gtccgttcaa taaaagcaac ggtcagcaaa accatttcgg cttcttttaa
     3421 atgtacaccg caataataaa tcagattggg cattagcatt gcaaaaatac ctgccattct
     3481 gccggttgat tcttcgaaat ttctggtagt aagtttataa attagaacac aagttatcgc
     3541 tccataaacc gcttttaata atcttgcaaa gagaatcgat ttacccgtaa ctgcatatag
     3601 gcaacccaga tagaagggat atcccatgtc ggaaaaacgg ccattaatat aggcaaaata
     3661 aggttgaata ttgcctttat gaatcatgtc agccaaccag accgcttcgt tgtgatatcc
     3721 ttgtgcatcg gcagctgcaa attcaaatgg ttgaccggtc atggattgat agaaaaaata
     3781 actgaatatt acccaaacta tcctgataat caaagaattg gtgaaaagtc gtttggtaaa
     3841 agttttcggt gaggttttac cccactgacg tgttagttcg ttggaaaaat aaaaaaagcc
     3901 gattacttcc accaatccga aaaatatcca tatccacgac atggcatggg aaaagaaaac
     3961 cactgttact gctgccaatg atatgacata cagcagcatg gccttttggg taaaatattt
     4021 cggaaaataa ttgagcattc ttttttggtt ataactaatc tggaaagttt taaattccaa
     4081 gttttgagtt acaagttacg agttatgagt aattttcatt tatcacttat cattcaacac
     4141 tatccattgt ttcatactct tgttttaact gacagatata tctttctcgc tgatttccgc
     4201 tgataagttt tctgcattca tctgcttttt tttatttgcg gttatctgcg agagattatt
     4261 tttttttctc gctgatttcc gccgattttt ttctcgctga ttttcgctga tttttattcg
     4321 ctgatttacg ctgataagtt tctgtgttta tctgcgtttt ttatctgtcg atcattcatt
     4381 agctttcttt gatgcagaaa ttaagttttc actatcttga acatacctat caggtgaata
     4441 tgctttgaac gaactgcagt aattaaagcc agaccgttaa accaactgat gaacaaaaca
     4501 ttaacaaaaa cgcattcacc catcacccat cactcatcat acatcaatca tcactatcca
     4561 atgtttcata cacttgtttt aactgacgga tatgactttc gatactgaaa ttttcgcgga
     4621 cataggctgc tgcttcacga gcctgacgaa ttgcaatcgc tctattttgc aaaaataaca
     4681 gaaatttttt taataaatcg tattcatctc tgcttttgta caaagtggca aattttccat
     4741 tttgtgtaat ttccttcatc acttcccaat cattcacgaa cacgggaatg cccattgcca
     4801 ttgcttctac aaccgcaatg ccaaaagtgt catggtcggt agaataaata aaggcatcaa
     4861 gagtgcttaa aatttcgggc acatcactgc gtgaacctaa aaaatgtacg ttttccagca
     4921 gattgttctc ttcacaataa tgataacaat catcgaaaag ctcgggttca gatttgcttt
     4981 tagctccgac aaaaacaaaa tcgaaagcaa tattttcttt tttcagcaaa ttcagaaatc
     5041 tgcaaatgac aatttgcgag cgtccactta caaaattacc cactgaaccc agttttaaaa
     5101 gttcggaaga agtcgatttt tttgttctat ccgtttttac tgtatccaat ttatcaaagg
     5161 atataccgtt gtaaacggtt ttttgttttt cgggtttcaa atgatatttt tgttgataat
//
LOCUS       DAOSCH010000127         5152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_10140_length_5152_cov_7.178536, whole genome shotgun sequence.
ACCESSION   DAOSCH010000127 DAOSCH010000000
VERSION     DAOSCH010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5152
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10140_length_5152_cov_7.178536"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..234)
                     /locus_tag="PLZ54_08130"
     CDS             complement(46..234)
                     /locus_tag="PLZ54_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66924.1"
                     /translation="MCLFQRTTDRQSTRKTEGQHPTAVWRNGGCSASYDSFVVGSSAV
                     LRLNFCAKNPPLRQAAKR"
     gene            complement(303..1430)
                     /locus_tag="PLZ54_08135"
     CDS             complement(303..1430)
                     /locus_tag="PLZ54_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOJ66925.1"
                     /translation="MITKEKILNGFETAEARWARFGPYYAMFPVEFAFDVVEKYSKKG
                     DYIIDPFAGRCSSIYAGGVLGRNSLGIEINPVGWLYGTVKLQPADKEDVIERLLEIYS
                     KRNNYSQALQQMPEFYRICYCDEVLKFLLAARDNLDWKNNNVDATLMSILLIYLHGKI
                     GEGLSNQMRMTKSMGMNYSVQWWKKNKMTTPPEINPCDFILKKIEWRYEKGKPKITES
                     AVVFGDSSNELVNIAEKAIKNNIKFSLLFTSPPYYSITDYHADQWLRLWLLGGSENPQ
                     SIKDKHKGRFVNKKEYYDLLDNVFGLCAKMMKRKSTVYVRTDKREFTFNSTLEILKKH
                     FPNHSVKIVEKPLTKDTKTQTKLYGDKTMKPGEVDIIMTRK"
     gene            complement(1427..2794)
                     /locus_tag="PLZ54_08140"
     CDS             complement(1427..2794)
                     /locus_tag="PLZ54_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HOJ66926.1"
                     /translation="MSFEGEFASYEPLRRLLDSEKVKSLQNRLKIRQQEEETEDFENS
                     IVKKSDLTESTLQPDLVLAIDGSNLAAKAENGFPGAEFGYITIASVLIDLKLIGELEK
                     KEFVEPKKFRETEKASTIESVFPGCNVILDTEKNAKASLRRALFEELRSNTVFSDGET
                     LLDTYEHLFRIKREHFQERNLPRSPIEGIDEEMTYDYGEYTCPHSGEPLFSTDALRLH
                     ELMNPGGSNGEMFGQIMATLEKLWLIHILRAFERKGWLATLRRVAFIMDGPLAVFSTS
                     SWLTKVISHELTRLNEIQKKINDQDLLIIGIEKSGTFFNHFIEIDTTKDGVTDKFPKQ
                     SALLLNDGYIKRNIIFSESPKQYGQDTYFGRKFFYKTASGQKIVPVVACFNEYQRNLE
                     TAYPDQFARLADVMNLLDRLVSNKYPNSVSPLVSAHAEAAIPLNIGKRIFEDIAREIR
                     EKSSK"
     gene            complement(2807..4906)
                     /locus_tag="PLZ54_08145"
     CDS             complement(2807..4906)
                     /locus_tag="PLZ54_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HOJ66927.1"
                     /translation="MSIEQGLNSRRENDKQAESQQWFQKLIQVDQYVGDIYSINYETA
                     RVIIHDFYREKVGGIPSLSFLIATRVDPIKTDIDFKKEDASFILLRVMDAAALPQDKE
                     AERIRVETAQRVSGETEKHWDDPGSMDLRTKNLLGFAGVECRIIGTFYLEENEHNSEA
                     PLNLKFGSDISNYYPNRGLKVYKPNGKALEQIVNYADPSSIQAHIEKYGNTERVKLGF
                     VRYASTNRKYQQIDDVPVYIYPADLLSQKSALFGMTRTGKSNTTKIIAKSVFELRLPK
                     KSDDKPLRIGQIIFDPNGEYANENVQDNNSALKNVWQLLPNGIKANEVVTYGITRHPN
                     DPDRTLMLLNFFEDENLQIGKEIIDSILSEDNTTYIKNFCQVSFEEPDAGDRSALTRH
                     NRRVLAYKSVLARAGFAIPQNQRPNTRNLFNRELLNAMQNSASDNAPEYQSAAQIFGN
                     PNPTWGQLANAFEALDKFIRDRNSGYQAFETAYVNRPNGSGDRWADEDLKKIIGIFQY
                     PNGTRKIGKAAEQHSADTTSDYAEDIYNHLVQGKLVIIDQSNGEPELNKSSATRIMTK
                     IFKENQRRFIQGATDIPEILVYVEEAHNILPAGNDLDLSDIWVRTAKEGSKYRIGMVY
                     ATQEVSSIQKNILKNTANWFISHLNNTDETKELCKYYDFADFEPSIRRAQDKGFLRVK
                     TLSNLFVIPVQVDKFEV"
BASE COUNT         1387 a          981 c          887 g         1897 t
ORIGIN      
        1 acgccttacc ctggcagcgg agctgctgaa tgttggggtg gtgagttaac gttttgcggc
       61 ttggcgtagt ggcggatttt tagcacaaaa attcaatcga agaactgcac ttgaacctac
      121 cacaaaactg tcatacgaag cactacaccc gccattacgc caaaccgctg ttgggtgctg
      181 gccttctgtc tttcgtgttg attgtcggtc agttgtgcgt tggaatagac acactctttg
      241 ccaagtttta gcttgtgcgt tggcttgtgt gacttggcaa tgtgtgtggc tgttgggttg
      301 gctcattttc ttgtcattat tatatcaact tctccgggtt tcattgtttt gtcaccgtag
      361 agttttgttt gtgtcttggt gtcttttgtt aaaggctttt caacaatctt tactgaatga
      421 tttggaaaat gcttttttaa gatttcaagt gtcgaattga aagtaaattc tctcttgtct
      481 gttcgcacgt aaacggtact ttttcttttc atcattttgg cacacagtcc aaatacattg
      541 tcaagcaaat cataatattc ttttttattt acaaatcgtc ctttatgttt gtctttaatt
      601 gattgaggat tttctgatcc tcctaaaagc caaagtctaa gccactggtc agcgtgatag
      661 tcagttattg aataatatgg tggtgaagta aatagtaatg aaaattttat attgtttttt
      721 attgcttttt ctgcgatgtt aactaattca tttgaactgt cgccaaatac aacagcactt
      781 tcagtaattt tcggctttcc tttttcataa cgccattcaa tttttttgag aatgaaatcg
      841 caagggttta tttctggtgg tgtagtcatt ttattttttt tccaccattg aactgaataa
      901 ttcattccca ttgatttagt cattctcatt tgattggata gaccttctcc aattttaccg
      961 tgcaggtaaa taagtagaat tgacattaga gttgcatcta cattattatt tttccaatct
     1021 aaattatcac gagcagctaa taggaattta agaacttcgt cacagtagca aatacgataa
     1081 aattccggca tttgctgaag ggcttgacta taattatttc ttttactata aatttcaagc
     1141 aatctttcga taacatcttc tttatctgct ggttgaagtt ttacagttcc ataaagccaa
     1201 cctacaggat ttatttcaat acccaaactg tttctaccca agacgccacc tgcatatatg
     1261 ctgctgcatc tacctgcaaa agggtcaatg atataatcgc ccttctttga atacttctcg
     1321 actacatcaa atgcaaattc cacaggaaac atagcgtaat acggaccaaa ccttgcccac
     1381 cgtgcttcgg ctgtttcaaa gccatttaat attttttctt ttgttatcat ttacttgatt
     1441 tttctctaat ctctcttgca atatcttcaa atattcgttt accaatattt aatgggatag
     1501 ccgcttctgc gtgtgctgaa accaaaggtg aaacggaatt cggatatttg tttgatacta
     1561 atcgatcaag aaggttcatt acatcggcta accttgcaaa ttggtcggga tatgcagttt
     1621 ctaaatttct ttgatattca ttgaaacaag caacaaccgg tacaattttt tgacctgatg
     1681 ctgttttata gaaaaacttt cgcccaaaat aggtatcttg tccatactgc ttaggacttt
     1741 cagaaaaaat tatatttcgt tttatgtagc cgtcatttaa aagtagggcc gattgtttcg
     1801 gaaatttgtc tgttaccccg tctttagtag tgtctatttc aataaagtga ttgaagaatg
     1861 tccctgattt ttcaatacca ataattaaaa ggtcttgatc atttattttt ttttgtattt
     1921 cgttcaatct ggttaattcg tgactaatta cttttgtcaa ccaagatgaa gtgctaaaga
     1981 cagctaaagg accatccata ataaaagcta ctctccgaag tgttgctaac catcctttac
     2041 gttcaaatgc cctaagaatg tgaataagcc aaagtttttc aagggtagcc attatttgtc
     2101 caaacatttc accattgcta ccacctggat tcattaattc gtgtagacgt aaagcatctg
     2161 ttgagaaaag tggctcacct gaatgaggac aagtatattc cccataatca taagtcattt
     2221 cttcgtcaat tccttcaatc gggcttcttg gtaaatttct ttcctgaaaa tgctcacgtt
     2281 ttattctaaa aaggtgctcg tatgtgtcaa gcaaagtttc accatctgaa aaaacggtgt
     2341 tgctacgcaa ctcttcaaat aatgctctcc taagagaagc tttagcattc ttttcagtgt
     2401 caaggattac attacagccg ggaaaaacac tttcaatggt tgatgctttt tcggtttcac
     2461 gaaatttctt tggttcaaca aattcctttt tttctaactc tccaattaat ttcaagtcaa
     2521 ttaaaacgga agcaatggta atataaccaa attcagcacc gggaaatccg ttttctgctt
     2581 ttgcagctaa attacttcca tcaattgcaa gaactaaatc aggttgtaaa gtactttcgg
     2641 tcaaatcaga ttttttgact attgaatttt caaaatcttc tgtttcttct tcctgttgcc
     2701 ttattttaag tcggttctga agtgatttaa ctttttcact atcaagtaaa cgtcttaaag
     2761 gctcgtaact ggcaaattct ccttcaaaac tcataatgat aatgctttaa acttcaaact
     2821 tgtcaacctg aacaggaata acaaataggt tacttaatgt tttaactctt aaaaagcctt
     2881 tgtcttgagc tcgcctaatg gacggttcaa agtctgcgaa atcgtaatac ttacaaagct
     2941 cttttgtttc gtcagtattg ttcaagtggc taataaacca attggcggta tttttaagaa
     3001 tgttcttttg aatactgctc acttcttgtg tggcataaac cattccaatg cgatacttgg
     3061 aaccttcttt tgcagttctt acccaaatgt cagataaatc taaatcatta cctgctggta
     3121 aaatgttgtg tgcttcttct acatatacca aaatttcagg aatgtcggtt gcaccttgaa
     3181 tgaaacgcct ttgattttcc ttgaatattt ttgtcataat tcttgttgca gaagatttat
     3241 tcaattcagg ttctccgttt gattggtcaa taattaccaa tttgccttga acaaggtgat
     3301 tataaatgtc ttcagcgtaa tcacttgtcg tgtcagcact atgttgctct gctgcttttc
     3361 caatttttct tgttccgttc gggtattgga aaatgccaat tatttttttt aaatcttcat
     3421 cagcccatct atcacctgaa ccatttggac gattaacata ggcagtttca aatgcctgat
     3481 acccactatt tctatctctt atgaatttgt caagtgcttc aaaagcattg gcaagttgac
     3541 cccaggttgg atttgggttc ccaaaaatct gtgctgctga ttggtattcg ggtgcattat
     3601 cgcttgcact gttttgcatt gcattcaaca gttctctgtt aaatagattc cttgtattag
     3661 gtctctgatt ttgcggtatt gcaaatcctg ctcttgctaa gactgattta tatgccaaaa
     3721 ctcttctatt atgtcttgtt aatgcacttc tatcgcctgc atcaggttct tcaaaactta
     3781 cttgacaaaa gtttttaatg tatgtagtat tatcttctga aagtatactg tcaataattt
     3841 ctttgccgat ttgtaaattt tcatcttcaa agaaatttaa aagcattaag gttctatctg
     3901 ggtcgtttgg atgtcgtgtt attccataag taacaacttc attagctttt attccatttg
     3961 gtaatagttg ccaaacattt tttagtgcgg aattattgtc ctgaacattt tcatttgcat
     4021 actcaccatt tgggtcaaaa attatttgtc caattcttag tggtttgtca tcagactttt
     4081 taggtaatcg tagttcaaaa acagatttag cgataatttt agtggtgttt gatttacctg
     4141 ttctggtcat accaaacaat gcagattttt gagaaagcaa atcagctgga tagatgtaaa
     4201 ccgggacatc gtcaatttgc tgatacttac gattagttga agcgtatcgg acgaaaccaa
     4261 gttttaccct ttctgtattt ccgtatttct caatgtgcgc ctgtatactt gatgggtctg
     4321 cgtaattaac tatctgttct agtgctttgc cgtttggttt ataaaccttc aatcccctat
     4381 taggatagta attagaaata tcacttccaa attttagatt taatggggct tcactattat
     4441 gttcattttc ttctaagtaa aatgttccaa taattcgaca ttcaacaccc gcaaaaccaa
     4501 gtaggttttt tgttctcaaa tccattgaac ctgggtcatc ccaatgtttt tctgtttcac
     4561 cgcttactct ttgagctgtc tcaaccctta ttctttctgc ttctttgtct tgcggcaaag
     4621 cagctgcatc cattacacgc aacaaaatga aggaagcatc ttctttttta aagtcaatat
     4681 cagttttaat tgggtcaaca cgtgtagcaa ttagaaaact taaacttggt attccaccaa
     4741 ctttttctct gtaaaagtcg tgaatgatta cccttgctgt ctcataattg atcgagtaga
     4801 tgtcaccaac atactggtca acttgaatta gcttttgaaa ccattgttgg ctttcagctt
     4861 gcttatcgtt ttctcgtctt gagttaagtc cttgttctat gctcatattg tcaatttctt
     4921 aattgtcaaa attattcatt tctgtcggtg gggtggcgaa aaatggctct tttggttttg
     4981 cgaagggtcg gcttttgtgt cgggcaaaac caaatgtgcc atttgtggga tggcatttat
     5041 ttcttttatg tcgtgtagtt aatttgttca ctgtcgttgt cttttaggct tgcacccaac
     5101 ggttacgtat atgaaacgtt tggcatttcg aagcactttc ctgtcaggtt ac
//
LOCUS       DAOSCH010000128         4980 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_10640_length_4980_cov_5.197970, whole genome shotgun sequence.
ACCESSION   DAOSCH010000128 DAOSCH010000000
VERSION     DAOSCH010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10640_length_4980_cov_5.197970"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="PLZ54_08150"
     CDS             <1..978
                     /locus_tag="PLZ54_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOJ66928.1"
                     /translation="SYLSFSRAINFIRFMGSYISTFLGINRSTKLSPFFISVEPADFC
                     NLQCPECPVGVKNSLLVERGNFFDLELYRNLIEEMKGTLLHVQFYFQGEPFLNKQLTE
                     MIRYAHDRKLFTSVSTNGQFLTDETAREIVRSGLDKLIVSVDGTTQEVYEMYRKGGNL
                     QKVFDGINHVIAWKRQLKSLFPMIEMQFLVLKSNEHQMQEMKQIAKNLNVDKLSFKSA
                     QFYDFENGNSQIPANSRYVRYRLNKSGKYELKFSQPNRCSRLLGGAVINAKGEVLPCC
                     YDKQSQYSFGNIRSASFYACWHSDKANSFREQLLLDRHQFDICRNCTGR"
     gene            complement(1059..3908)
                     /locus_tag="PLZ54_08155"
     CDS             complement(1059..3908)
                     /locus_tag="PLZ54_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005923870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOJ66929.1"
                     /translation="MNLKTILENRILILDGAMGTMIQRYRLDESAYRGERFSHWNKQL
                     KGNNDLLSLTQPDIISSIHKQYLQAGADIIETNTFNAQRISMEDYGMGEFVREMNLSA
                     ARIARQAADEFTLKNPEKPRFVAGSVGPTNKTASLPSDVNRPASRAVTFDQLVTAYKE
                     QILALIEGGVDALLIETIFDTLNAKAAIFAAQEAMSELNKKVELMISATIADASGRTL
                     SGQTLRAFMASVAHAPLLSIGLNCSFGAKDLKPYLQEMSAHAPWFVSAYPNAGLPNQF
                     GEYDETPDTMAKQVKEYIVEGLVNIIGGCCGTTPEHIAKYRDLIANAAVRKPKEKTVN
                     LELSGLELLEIISPKIDDRQQKESIEATNSTKEKVSRNNSQEIIDISTDPAPKSLFVN
                     IGERCNVAGSRMFLRLINEKKYEEALHIARKQVEDGAQVLDINMDDAMLNAAEEMAVF
                     LNHITAEPEIARVPMMIDSSKWEVIEAGLKCIQGKSIVNSISLKEGEEDFLKKAKKVR
                     AYGAAAVVMAFDEKGQADSLERRIEICSRAYRLLTEKAGFPPQDIIFDPNVLAIGTGI
                     EEHNHYAVDFLESVRWIKQHLPYAKVSGGISNLSFSFRGNNTVREAMHSAFLYHAIKA
                     GLDMGIVNAGALPIYEEIEPELLEKVENVIFNRKATATEELIEYAEKIKNTVSEKKSD
                     KKDEWRSLPVAKRLEHALVKGIDEFLDTDLAEALTIYDTALEIIEKPLMDGMNIVGKL
                     FGEGKMFLPQVVKSARTMKKAVTILQPEIEKQKVAGTASSAGKFLIATVKGDVHDIGK
                     NIVSVVLACNNYEVIDLGVMVPSDKIIQTAINENVDLVGLSGLITPSLEEMSNVAAEM
                     EKAGLNVPLLIGGATTSKVHTAVKIAPHYHGPVVYVKDASVNTFIVSQLMNKKTHSHF
                     VSEIAAEYQEIREKQVQKTEVLSLEEARKRKPNFF"
     gene            4122..4868
                     /gene="gpmA"
                     /locus_tag="PLZ54_08160"
     CDS             4122..4868
                     /gene="gpmA"
                     /locus_tag="PLZ54_08160"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOJ66930.1"
                     /translation="MKKLVLVRHGESVWNKENRFTGWTDVDLSEKGMQEAAKAGRLLK
                     EKGFFFDKAYTSYLKRAIKTLNIILDVMDLDWIPVEKSWRLNEKHYGMLQGLNKAETA
                     EKYGEEQVQIWRRSYDVPPAPLDMNDPRSPFLDPRYKNVDKKDLPLTEALKDTVARIL
                     PYWENNIFPSLKENNEILVVAHGNSLRGIVKVLKNISDEDIVGLNLPTGVPYVFEFDD
                     NYQVVNDYFLGDPEEIKKLMEEVANQAKKK"
BASE COUNT         1406 a          973 c         1009 g         1592 t
ORIGIN      
        1 tcttatttaa gcttttctcg tgcaatcaat ttcattcgat ttatgggttc ctatatttcg
       61 acttttttgg gtataaaccg atctactaaa ctgagccctt ttttcatatc ggttgagccg
      121 gccgattttt gtaatttgca atgtcctgaa tgtcctgtgg gtgtgaaaaa ttccttattg
      181 gtggaaagag gaaatttttt tgatttggag ctttacagaa atttgataga agaaatgaaa
      241 ggaactttgc tgcatgtaca attttatttt cagggagagc cttttttgaa caaacaactc
      301 actgaaatga ttcgatatgc ccatgataga aaattattta cttccgtgtc gaccaacgga
      361 caatttttga ctgacgaaac agcacgtgaa atagtgaggt cgggattgga taaactcatc
      421 gtttcagtcg atggcaccac ccaggaagtt tatgaaatgt acagaaaagg gggaaatttg
      481 cagaaagtat tcgatggtat caatcatgtt atagcttgga aaaggcagtt gaaatcttta
      541 tttccaatga ttgaaatgca gtttttggtt ctaaagtcca acgaacatca aatgcaggaa
      601 atgaaacaaa tagcaaaaaa cttgaatgtg gacaaactta gttttaagtc cgcacagttt
      661 tacgattttg aaaatggaaa ttcgcaaatt cctgccaaca gtcgttatgt gaggtatcga
      721 ctcaacaagt caggaaaata cgaacttaaa ttttcgcagc ccaatcgatg cagtcgtttg
      781 ctgggaggag cagtaattaa tgcaaaagga gaggtgttgc cgtgttgcta tgataagcag
      841 tcgcaatact catttggaaa tatccgttct gcttcctttt atgcctgttg gcattcggat
      901 aaggcaaatt cttttcggga acagttgctc ttagaccgtc atcagtttga tatttgcaga
      961 aattgtacgg gaagataaag aattgcggct gaaaagggtt acgagttgtg agttctaagt
     1021 cgttcatgcc tgaaatacgt tgaccatttt ggttattttc agaaaaaatt tggtttacgt
     1081 tttcgagctt cttcaagtga gagcacttca gttttctgaa cttgcttttc gcgaatttcc
     1141 tgatattcgg cagctatttc ggaaacaaaa tggctatgtg tttttttgtt catcagctga
     1201 gaaacgatga aagtattgac ggatgcatct ttaacatata ccaccggtcc atgataatga
     1261 ggcgcaattt ttactgccgt atgaactttg gatgttgttg ctcccccaat aagtaaggga
     1321 acgttgagcc cggctttttc catttcggca gcaacgttgc tcatctcttc cagcgaagga
     1381 gttatcagtc cacttaatcc aaccaaatcg acattttcgt tgattgctgt ctgaataatt
     1441 ttatctgagg gtaccataac acccaagtcg ataacttcat aattgttgca agccaatacc
     1501 acagatacaa tattttttcc aatatcgtgc acatcacctt ttacggttgc aataaggaat
     1561 ttgcctgccg atgaggctgt tccggcaact ttttgttttt caatttcggg ctgaagaatg
     1621 gttacggctt ttttcatggt tcgagccgac ttcaccactt ggggcaaaaa cattttacct
     1681 tctccgaaaa gttttccaac aatgttcatc ccgtccatca agggtttttc gatgatttcc
     1741 agtgcggtat cgtaaatagt cagtgcttct gccaaatcgg tatcgagaaa ttcatcaatt
     1801 ccctttacaa gtgcatgttc aaggcgttta gcaaccggaa gcgaacgcca ttcatctttt
     1861 ttgtccgatt ttttttcgga caccgtattc tttatttttt ctgcatattc tatcagttct
     1921 tccgtagctg ttgctttccg gttgaagata acattttcca ctttttccaa aagttcaggc
     1981 tctatttctt cataaattgg cagcgctccc gcgttaacaa ttcccatatc caatccggct
     2041 tttatggcgt gataaagaaa agcggaatgc atggcctcac gaaccgtatt gtttcctctg
     2101 aaggaaaaag ataaattgct gattcccccg ctaacttttg cataaggcaa atgttgtttg
     2161 atccatcgta ccgactccag aaaatctaca gcgtaatgat tgtgttcttc tattccggta
     2221 ccaatagcca aaacattggg atcaaaaatt atatcctgtg gtggaaatcc tgctttttcg
     2281 gttagcagac gatatgctcg ggagcaaatt tcgattcttc gctccaagct gtcggcttgg
     2341 cctttttcgt cgaaagccat cacaacggca gccgcaccat aagcccgcac cttttttgct
     2401 tttttcagaa aatcttcttc tccttccttt aaactgatgg aattgacaat gctttttccc
     2461 tgaatacatt tcaaaccggc ttcaatgact tcccattttg atgaatcaat catcatggga
     2521 acacgtgcaa tttcgggctc ggcagtaatg tgattcaaaa aaacagccat ttcttctgct
     2581 gcattcagca tggcgtcatc catgttgatg tcaagtactt gcgcaccatc ttcaacttgt
     2641 tttcgggcaa tgtggagcgc ttcttcgtat tttttctcgt tgattagcct taaaaacatg
     2701 cgcgaaccgg ccacgttgca tcgttcacca atgttcacaa ataaagattt tggagccgga
     2761 tcggttgaaa tgtcgataat ttcctgacta ttatttcttg aaactttttc ttttgtcgaa
     2821 tttgtggctt caatgctctc cttttgttgc ctatcgtcaa ttttaggaga aatgatttcc
     2881 aatagttcca aaccgctcag ttccaggttt acggtttttt ctttgggttt tctgaccgct
     2941 gcattggcaa tcagatcccg atatttggca atatgttccg gagttgtacc gcagcaaccc
     3001 ccgataatgt tgaccaaacc ttcgacaata tattctttta cttgttttgc cattgtgtcg
     3061 ggcgtttcgt cgtattcacc aaactgattc gggaggccgg cattggggta tgcactgaca
     3121 aaccaaggtg catgagcgct catttcctga aggtaaggtt ttaaatcttt ggctccaaaa
     3181 gaacagttta atccgataga aagtagcgga gcatgtgcca cggaagccat aaaagcccga
     3241 agtgtttgcc cggaaagtgt acgtccgctg gcatcggcaa tggtagccga aatcattaat
     3301 tccacttttt tattcaattc cgacatagct tcttgagccg cgaaaatagc ggcttttgca
     3361 tttaaagtgt cgaaaatggt ttctatcaac agcgcatcta ctccaccttc tatcaaagcc
     3421 agtatttgtt ccttgtaagc tgttaccagt tgatcgaagg ttacggcgcg cgaagcggga
     3481 cgatttacgt cggaaggtaa ggaagccgtt ttattggttg ggcctaccga accggcaaca
     3541 aatcttggtt tttcggggtt tttgagagtg aattcatctg ctgcctgtct ggctatgcgc
     3601 gctgcactca aattcatctc tcttacaaat tctcccattc cataatcttc catcgaaatt
     3661 cgttgtgcat tgaaagtgtt ggtttcgata atatcggcac ctgcttgcaa atattgctta
     3721 tggatggaac taataatatc cggttgagtt aaagaaagaa ggtcgttgtt tcctttcaat
     3781 tgcttgttcc aatgagaaaa acgttcacct ctgtatgcgg attcgtcgag cctgtaacgc
     3841 tgaatcatgg tgcccattgc tccatccaga atcaatatgc gattttcgag aattgttttt
     3901 aaattcatag tgttgtaaaa taggaaggaa attttcaaat atatctgtcg ttttatttct
     3961 gcaaaattaa tgaatatggg tcaattctca aaaacagcaa cttatcctta taaatttcca
     4021 ttctatgatt tttattttcg cacgtttgga aatacttcga ttttcatgta attttgtcat
     4081 caataaaaaa agtgaatcac taacctctta ataagaagaa aatgaaaaaa ttagttttag
     4141 taagacatgg cgaaagcgtt tggaataagg aaaatcgctt tactggttgg acagatgttg
     4201 atttgtctga aaaaggaatg caagaagcag ccaaagcagg cagattgctg aaggaaaagg
     4261 gtttcttttt cgataaagcc tacacttcct atttgaagag agccattaaa actttgaata
     4321 tcattcttga tgtaatggat ttggattgga ttccggtaga aaaaagctgg cgtttgaacg
     4381 aaaaacatta tggaatgctt cagggtttaa acaaagcaga aacagccgaa aaatacggcg
     4441 aagaacaagt tcagatttgg agacgcagct atgatgttcc gcctgcaccg ctggacatga
     4501 atgatcctcg ttcaccgttt ttggatccac gctacaaaaa tgtggataaa aaagatttgc
     4561 ctctgacaga agctttgaaa gataccgtag ctcggattct tccttattgg gaaaacaaca
     4621 tcttcccaag tttgaaagaa aacaacgaaa ttctggttgt tgctcacggt aacagcttga
     4681 gaggtattgt aaaagttttg aaaaatattt ccgacgaaga tatcgtgggt ttaaatctcc
     4741 ccacaggtgt gccctatgtt tttgaatttg atgataatta tcaggttgta aacgattatt
     4801 ttctgggcga tccggaagaa atcaaaaaat tgatggaaga agtggccaat caggcaaaaa
     4861 agaaataaga agaattcttc tcttctaagc tataataaag cagtgcacaa ccgtactgct
     4921 tttttattat atacattaca ttaaattatt gcacaaataa attattatct ttgtctcata
//
LOCUS       DAOSCH010000129         4938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_10768_length_4938_cov_4.132705, whole genome shotgun sequence.
ACCESSION   DAOSCH010000129 DAOSCH010000000
VERSION     DAOSCH010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10768_length_4938_cov_4.132705"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..486
                     /locus_tag="PLZ54_08165"
     CDS             10..486
                     /locus_tag="PLZ54_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOJ66931.1"
                     /translation="MNISLGEVELKNDLTETEKTNLDNHLKMFGFELIDDKKSRLIGQ
                     IKSSIIEIIHQNNSELNSNLSDYLSSKLHHDYTYLSNLFSEVEGTTIEKYFIAQKIEK
                     VKELLVYDELSLSEIAYQMNYSSVGYLSNQFKKVTGLTPTHFKNIKEIKRKPLDEV"
     gene            570..2981
                     /locus_tag="PLZ54_08170"
     CDS             570..2981
                     /locus_tag="PLZ54_08170"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOJ66932.1"
                     /translation="MATNNNEFRIPLEGVESEHCALIVDNGLAKLNGVESHRVELNNK
                     EAIIETQNRETVSEAVKTIRDLGYGVTTVKKSFPVLQMTCASCAISVESMLKSQAGVV
                     NASVNYANAKVSVEFIPSLIQVEHLRKVVQSIGYDLLIDESPSGDETIEHIQKENFKT
                     LKKKTYWALILSIPVVVIGMFFMNMPFANEIMWFLSTPVLFWLGRDFYINAWKQAKHR
                     SANMDTLVALSTGVAYIFSVFNTLFPEFWHERGLHAHVYFEAASVIIGFILLGKLLEE
                     KAKGNTSSAIKKLMGLQPKTVTIVQDGGYQMEIPIEQVEIGNTIVVKPGEQIAVDGIV
                     INGNSFVDESMLSGEPVPVLKNENDKVFAGTINQKGSFQFKADKVGSETMLAHIIKMV
                     QDAQGSKAPVQKLVDKIAGIFVPIVIVIAVASFIVWNIFGGDNGFTQGLLALITVLVI
                     ACPCALGLATPTAIMVGVGKGAEKGILIKDAESLELAKKVNAVILDKTGTITEGKPVV
                     TNDFWTEETPILKQILFSIEKQSEHPLADAVVSHLKNQNTVSITQFESITGKGAKAEI
                     DGITYFIGNKRLIQENEILIENAVAQKANQWGNEAKTVIWFANTKKTIAILAIADQIK
                     ATSKKAIEELQLSGIEVYMLTGDNEMTAKAVSEKVGIKHYKAEVLPEHKAAFVKQLQQ
                     QGKVIAMVGDGINDSTALAQADVSIAMGKGSDIAMDVAKMTIISSDLTKIPEAIHLSK
                     STVRTIKQNLFWAFIYNLIGIPIAAGILYPFNGFLLNPMIAGAAMALSSLSVVSNSLW
                     LKYKK"
     gene            3079..3306
                     /locus_tag="PLZ54_08175"
     CDS             3079..3306
                     /locus_tag="PLZ54_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOJ66933.1"
                     /translation="MKTNDKTLKFKTNINCSGCVEKVTPFLNDANGICHWDVDTANKD
                     KILSVHSEGITEEEVIQKVQDAGFKIELLTQ"
     gene            3317..4171
                     /locus_tag="PLZ54_08180"
     CDS             3317..4171
                     /locus_tag="PLZ54_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013632648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3347 domain-containing protein"
                     /protein_id="HOJ66934.1"
                     /translation="MNIVSKISAATLLLFSSSSCNAQIKNAKTEIVKIYGNCEMCEKT
                     IETAGNVKKVANVEWNKDSKMATITYDSIKTNQDEILKRIALAGYDSDKFLAPDDVYS
                     KLAGCCQYERVKKTAIVSTAVIEDHSMHNQENVVETKQEVNQLKTIFESYFALKDALV
                     KSDGKLVSTLAKDVLANINSVKMEKLSSEEHTVWMKVMSSLKSNTEKIVATTIIEKQR
                     VVFMDLSANFYALLKVSKQDYSIYYQNCPMYNDGKGANWLSKENAVKNPYYGSQMLTC
                     GKTVETIK"
     gene            4238..>4938
                     /locus_tag="PLZ54_08185"
     CDS             4238..>4938
                     /locus_tag="PLZ54_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002978414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HOJ66935.1"
                     /translation="MVLQNILSKKLLPIVFLLLVSNNISAQKTVRYDLYVKDTLVNFT
                     GKFKRAIAVNGQIPMPTLVFTEGDTAEIYVHNLLKEETSLHWHGLFLPNKEDGVPNLT
                     QMPIKPNTTHVYRFPIIQNGTHWYHSHSGLQEQIGMYGNFIMLKKSDDKTFRKGIDDL
                     PTVPIVLSEWTDLKPENIHRMLHNANDYPALKKNAVQSYAEAIKAGHFKTKLKNEWKR
                     MLAMDVSDVYYEKIL"
BASE COUNT         1831 a          795 c          946 g         1366 t
ORIGIN      
        1 tttcaaagtt tgaacattag tttaggcgaa gttgaactca aaaacgactt gactgaaact
       61 gaaaaaacta accttgacaa tcatctcaaa atgtttggat ttgaattgat tgatgacaaa
      121 aaaagtcggc taatcggaca aattaagtct tccattattg aaattataca ccaaaacaac
      181 agcgaattaa actcgaattt atctgactat ttgagcagta aacttcatca cgactatact
      241 tacttatcaa atctgttttc cgaagttgaa ggaacgacta ttgaaaaata tttcattgct
      301 caaaaaattg agaaggtgaa agaacttttg gtttatgacg aattatcatt aagcgaaatt
      361 gcttatcaaa tgaattattc gagtgttgga tatttgagca atcagtttaa aaaagttaca
      421 ggacttaccc ctactcactt taagaatatc aaggaaataa agcgcaaacc actagacgaa
      481 gtgtaaatgt tacaaataca atccaaaatc aggtaacggg taagaaatgt aatgttgcca
      541 cctttgcctt gtaattaaat aaaaaaggta tggcaacaaa taataatgaa tttaggatac
      601 cacttgaagg cgttgaaagt gaacactgtg ctttaattgt ggacaatgga ttggcaaaac
      661 ttaatggtgt tgaatcacac cgagtggaac tcaacaataa ggaagcaatc attgaaactc
      721 aaaatcggga aacagtttca gaagctgtaa aaactattcg agatttgggt tatggagtaa
      781 ctaccgtaaa gaaatcattc cctgtattac aaatgacgtg tgcttcttgt gcgattagtg
      841 tggagagtat gcttaaatct caggcaggag ttgtgaatgc aagcgtgaat tatgcaaatg
      901 caaaggtctc agtagaattt ataccgagtt tgatacaagt agagcatttg agaaaagttg
      961 ttcaaagtat tggttacgat ttgttaattg atgaaagtcc ttctggtgat gagaccattg
     1021 agcatattca gaaagaaaac tttaaaactc tcaaaaagaa aacctattgg gcgttgatac
     1081 tatccattcc tgtagtagta atcggaatgt tttttatgaa tatgcccttt gcgaatgaaa
     1141 taatgtggtt tttaagtacc ccagttttat tttggttagg gagagatttt tacatcaatg
     1201 cttggaaaca agccaaacat cgatctgcca atatggatac tttggtggca ttaagcacag
     1261 gtgttgctta cattttcagt gttttcaaca cactgtttcc tgaattttgg cacgaaaggg
     1321 gattacacgc tcacgtttat tttgaagctg catctgttat cattggcttt atattattgg
     1381 gtaaactatt ggaagaaaaa gccaaaggaa atacttcatc tgcaataaag aaattgatgg
     1441 gtttacagcc aaaaacagta acgattgtac aggatggtgg ttatcaaatg gaaatcccca
     1501 ttgagcaagt tgaaataggt aatacaattg tggtaaaacc aggcgagcaa attgcggtag
     1561 atggaatagt aattaacgga aactcttttg ttgacgaaag tatgttgagc ggtgagcctg
     1621 ttcctgtgtt gaaaaatgaa aacgacaaag tatttgcggg aactattaat caaaaaggta
     1681 gcttccaatt taaagccgat aaagtaggca gtgaaactat gcttgcacac attatcaaaa
     1741 tggtgcaaga cgcacaaggc agtaaagctc ctgtccaaaa attggtggat aaaattgcag
     1801 gaattttcgt gcccattgta attgtaattg ccgttgcttc atttatagta tggaacatct
     1861 ttggcggaga caatggcttt actcagggat tgttagctct tattaccgta ttggttattg
     1921 cctgtccttg tgctttaggt ttagctactc cgactgcaat tatggttggt gtgggcaaag
     1981 gagctgaaaa aggaatctta attaaagatg cagaaagtct tgagttagca aaaaaggtaa
     2041 atgctgtaat actcgataaa acaggaacca tcacagaagg taaacctgtt gtaacgaatg
     2101 atttttggac tgaagaaacc ccgatattaa aacaaatact attcagcatt gaaaaacaat
     2161 ctgaacaccc attggcagat gcggttgttt ctcatttaaa aaatcagaat accgtttcaa
     2221 taactcaatt tgaaagtatc acaggaaaag gagcaaaagc tgagattgac ggtataactt
     2281 atttcattgg aaacaaaaga ctgatacaag aaaatgaaat tttgattgaa aatgcggttg
     2341 ctcaaaaagc caatcaatgg ggcaatgaag ccaaaacagt aatttggttt gccaatacga
     2401 aaaaaacaat agccatatta gcaatagctg accagattaa agcaacttct aaaaaggcaa
     2461 ttgaagaatt acagctatca ggcattgaag tttatatgct cacaggcgac aatgaaatga
     2521 cagccaaagc tgtttcagaa aaagttggta tcaaacatta caaagcagaa gtactgcctg
     2581 aacataaagc tgcattcgtt aaacaattgc agcaacaagg aaaggttata gcgatggttg
     2641 gcgatggtat caatgacagc acagcattgg cacaagcaga cgtgagtata gcaatgggca
     2701 aaggaagcga cattgcaatg gatgtagcta aaatgactat catttcatca gaccttacaa
     2761 aaattcccga agcaattcat ttatcaaaat caacagttcg caccattaaa caaaacctgt
     2821 tttgggcatt tatttacaat ttgattggta ttccaattgc agcagggatt ttatacccgt
     2881 ttaacggttt cttactcaac ccgatgattg caggtgcggc aatggcattg agcagtttaa
     2941 gtgtagtaag taatagtctg tggttgaagt ataaaaaata agcagacgta aatgttacaa
     3001 atcaattcaa aaatacagta atgtatttag tttgaatgct atcgaaattt gcagtatata
     3061 atttaaacaa acattaaaat gaaaacgaac gataaaacat taaaattcaa aaccaacatc
     3121 aactgtagtg gatgtgtgga aaaggtaact ccatttctaa atgatgcaaa cggaatttgc
     3181 cattgggatg tggacactgc aaacaaagac aaaattcttt ctgtgcattc cgaaggaatt
     3241 accgaagaag aagtaatcca aaaggtgcaa gatgcaggat ttaaaattga attattaacc
     3301 caataaaaaa acagatatga acatagtatc aaaaatatca gctgcaactt tattgctgtt
     3361 ttcatcctcc tcttgcaacg ctcaaattaa aaatgcaaaa accgagatcg tgaaaattta
     3421 tggcaattgc gaaatgtgtg aaaagacgat tgaaacagca ggaaatgtaa agaaagttgc
     3481 taacgtagaa tggaacaaag acagcaaaat ggcaacaata acttacgaca gtatcaaaac
     3541 aaatcaagat gaaatcttga aacgcattgc tttggctggt tatgatagcg acaaattcct
     3601 cgctccagat gatgtgtata gtaaacttgc tggatgttgc caatacgaaa gggtaaagaa
     3661 aactgcaatt gtttcaaccg cagttattga agaccactca atgcacaatc aagaaaacgt
     3721 agttgaaacg aagcaagaag taaatcaact gaaaacgatt ttcgaaagtt actttgcttt
     3781 aaaagatgct ttggtaaaat cagacggtaa attagtttca acattagcta aagacgtact
     3841 tgcaaatatc aattcggtta aaatggaaaa actctcatca gaagaacaca ctgtttggat
     3901 gaaagtgatg agtagtttaa aatcaaatac cgaaaaaatt gtagctacta caattataga
     3961 aaaacagcgt gtcgttttta tggatttgtc agcaaacttt tatgctttgc tcaaagtcag
     4021 taaacaggat tattctatat actatcaaaa ttgcccaatg tataacgatg gtaaaggagc
     4081 aaattggttg agcaaagaaa acgcagtaaa aaatccatac tacggttcgc aaatgcttac
     4141 ttgtggcaaa acagtagaaa ccattaaata aactccaatt tggcgtaatc gaaaacggtt
     4201 acgccatttc ttaaactcaa caaatgaaaa tcttaaaatg gttctacaaa atattctttc
     4261 aaaaaaattg ctgccgatag tatttttact attggtcagt aataatatat ccgctcaaaa
     4321 aacagttcgt tacgaccttt atgtaaaaga cactttggta aactttactg ggaaattcaa
     4381 aagagccata gccgtaaacg gacaaatccc tatgccaacg ctcgtcttta cagaaggcga
     4441 cacagcagaa atttacgttc ataatttgct taaagaagaa acatctttgc attggcacgg
     4501 cttattttta cccaacaaag aagatggcgt tcctaatttg acgcaaatgc caatcaagcc
     4561 aaatactaca cacgtttacc gttttccaat tattcaaaat ggcacacatt ggtatcacag
     4621 tcattcgggt ttgcaagaac aaataggaat gtatggcaat tttataatgc tcaaaaaatc
     4681 tgatgacaaa acatttcgaa agggaattga tgatttacct accgttccca ttgtattaag
     4741 cgaatggaca gacttaaaac ctgaaaacat tcacagaatg ttgcataatg ccaacgatta
     4801 cccagcattg aaaaaaaatg ccgttcaaag ttatgcagaa gccattaaag caggacattt
     4861 taaaaccaaa ctaaaaaacg aatggaaaag aatgttagca atggatgtaa gcgatgtgta
     4921 ttacgaaaaa atattgat
//
LOCUS       DAOSCH010000130         4920 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_10817_length_4920_cov_6.728058, whole genome shotgun sequence.
ACCESSION   DAOSCH010000130 DAOSCH010000000
VERSION     DAOSCH010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10817_length_4920_cov_6.728058"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1764)
                     /gene="groL"
                     /locus_tag="PLZ54_08190"
     CDS             complement(136..1764)
                     /gene="groL"
                     /locus_tag="PLZ54_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOJ66936.1"
                     /translation="MAKEILYNHDAREQLKKGVDELANAVKVTLGPKGRNVIIEKKFG
                     APHITKDGVTVAKEIELEDPFKNLGAQLLKEVASKTSDDAGDGTTTATVLAQAIVSVG
                     LKNVTAGANPMDLKRGIDKAVSQVVKHIASQSKKVDDDYNKIEQVATVSANNDSEIGK
                     LIADAMRKVSKDGVITIEEAKGTETTIELVEGMQFDRGYISAYFVTNTEKMLVEMEKP
                     YILLYDKKISNFKELLPILEPVAQSGRSLLIIAEDVDSEALTTLVVNRLRGSLKVCAV
                     KAPGFGDRRKEMLEDIAILTNGVVISEEKGLQLEKATMDMLGTAEKVTVDKDNTTIVN
                     GAGSKEAIAARVSQIKSQIANTTSDYDREKLQERLAKLAGGVAVLYVGAPSEVEMKEK
                     KDRVDDALHATRAAIEEGIIPGGGVAYIRAIEALENLKGENEDEETGIEIVKRAIEEP
                     LRQIAFNAGKEGAVIVNKVKEGKGDFGYNARNEKFENFFETGVLDPAKVARIALENAA
                     SVAGMFLTTECVITEKKEENPVPQMPGGGMGDMM"
     gene            complement(1838..2110)
                     /locus_tag="PLZ54_08195"
     CDS             complement(1838..2110)
                     /locus_tag="PLZ54_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005842331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOJ66937.1"
                     /translation="MNIKPLADRVLVKPAPAEEKTSSGIIIPDSAKEKPLKGEVVAVG
                     KGTKDEEMIVAVGDTVLYGKYAGTELEWDGEKYLIMRQSDIMGIID"
     gene            complement(2283..3191)
                     /locus_tag="PLZ54_08200"
     CDS             complement(2283..3191)
                     /locus_tag="PLZ54_08200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ66938.1"
                     /translation="MLSILIPVFNYSVSRLVGELHRQALQLFIDFEIIVMEDGSNEYL
                     EENRQIDKLSRCSYIVLEKNIGRSAIRNRLADMSKYGHLLFLDCDAEVCSAYFVEKYV
                     SFCREESVVVGGTAYDENFNDFAYSLRLKYGRLREARPATLRNKLELSGKSNFSAFNF
                     LIAKSIFNRIRFDETISGYGHEDTLFGHRIHELGFNVFHIDNPLIHKGLDDNPTFLFK
                     TEEGIRNLYYLYKTGRYPFLAKESSLLNSFLTLKKLSLIEFFSFLYRIFRKSIYANLC
                     SSNPSLILFDFYKLLYFCNYSQKMTR"
     gene            complement(4009..4830)
                     /locus_tag="PLZ54_08205"
     CDS             complement(4009..4830)
                     /locus_tag="PLZ54_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66939.1"
                     /translation="MKKHFFIVLFCWAGAFSLLTAQDTIVFKKGEIKACKITQIKAGE
                     LTCKDFSYLDGPDYVYELKMIQEIHFSNGVVQIYNLPDVSEIKEEKGSITVSRGRIRN
                     LTQDSWTPPISFKTYKEKENYYKDQLYFPKFGDPYQPWVAGIASYLIPGLGQITCGEV
                     GRGFAFMGGAAASFFVFMIGALGSSGYYNDYYYRSDADFYPFLALSGVVSLFVIDIWS
                     VVDAFHVAKINNLYQRDLHSQEQVKLQLTPYIDKNKLDFSWNHQHTMGLSLQVNF"
BASE COUNT         1567 a          985 c          787 g         1581 t
ORIGIN      
        1 tttattacga aatttgtata accaaaaagt tgcatttgtg acttgaattc agcataagaa
       61 aaatttcaga cacaaaaaaa tctccgaaaa ctatacagtc tccggagatt tttaacctat
      121 cgagaatttt taaaattaca tcatatcacc cattccccca cctggcattt gaggaaccgg
      181 attctcttct ttcttttcgg tgatcacaca ttcggttgtc aggaacatac cggccacaga
      241 ggctgcattt tccaaagcaa tacgggcaac tttggcagga tcgagtactc ccgtttcaaa
      301 gaaattttca aatttttcgt tgcgagcatt gtacccaaaa tcgcctttgc cttctttaac
      361 tttgtttaca attacggcgc cttcttttcc ggcattgaat gcaatctgac gaagcggttc
      421 ttcgatagcc cgtttaacaa tttcgatacc tgtttcttcg tcttcgttct cacctttcag
      481 gttttccagc gcttcgatgg cgcgaatata agcaacacca ccacccggga taataccttc
      541 ttcgatggct gcacgagtag catgtaatgc atcatcaacg cggtctttct tttccttcat
      601 ttcaacttcc gatggagctc ccacatacaa cacagccact ccgcctgcca atttggccaa
      661 acgttcctgc aatttttcgc gatcgtaatc ggaagtagtg ttggcaatct gcgattttat
      721 ctgactcacg cgtgctgcaa tagcttcttt tgaaccggct ccgtttacaa tagtagtatt
      781 gtccttgtca accgtaactt tttcggctgt tccgagcatg tccatagtag ctttttcaag
      841 ctgcaaacct ttctcttcgc taattacaac accattggta agaattgcga tatcttcgag
      901 catttctttc cggcgatcac caaatcctgg agcttttacg gcgcaaactt tcaaagaacc
      961 acgcaaacgg tttacaacca acgttgtaag tgcttcactg tcgacatctt cggcaataat
     1021 caataacgaa cgacctgatt gtgccacagg ctcgagaata ggaagtaatt ctttgaaatt
     1081 ggatattttt ttgtcgtaaa gcaaaatata tggtttttcc atttcaacca acattttttc
     1141 ggtattggta acaaagtaag ccgaaatata accccgatcg aattgcattc cttccaccag
     1201 ttctatggta gtttcggttc ctttggcttc ttcaatggta ataacaccat ctttggagac
     1261 tttacgcatg gcatcggcaa tcagtttacc gatttcggag tcgttgttgg ccgaaacggt
     1321 agcaacttgt tcaattttgt tgtaatcgtc gtctactttt ttcgattgag aagcaatatg
     1381 ttttacaact tgagacacgg ctttatcgat tccgcgtttc aaatccattg gattggcccc
     1441 ggctgtaacg tttttcagtc caacacttac aatggcttga gccaacactg tagctgttgt
     1501 agttccatca ccggcatcat cgcttgtttt ggaagctact tcctttaaaa gctgagcgcc
     1561 caggtttttg aatggatctt ccagctcaat ttcttttgct accgtaacac catctttagt
     1621 tatatgaggt gcaccaaatt ttttttcgat tatgacattt cttcctttag gtcccaatgt
     1681 tacttttact gcattagcca attcatcaac acctttcttg agttgctcac gagcatcgtg
     1741 gttatataaa atttctttag ccatattctt tatgttttaa tttaataaac gtttttttaa
     1801 atttatattt aagttattaa ttatcaagct ttcttgctta atctattatt cccataatat
     1861 ccgactggcg cataataaga tatttttctc catcccactc cagttcggta cctgcatatt
     1921 tgccatataa cacagtatct cccacagcaa caatcatttc ctcatctttt gttcctttcc
     1981 caacagctac cacttcacct ttcagtggtt tttctttagc tgaatcggga ataataattc
     2041 cacttgaagt tttttcttct gccggagcag gttttaccaa cacacggtct gctaaaggtt
     2101 taatattcat atcacactaa tttttaaatg gttaataaat aaatttatat tttttgtcgt
     2161 atttttcgat acaaataaat aaattcgcta ctgcctgaat caaaatgtgt gcctgataaa
     2221 aaaatagatg atattgacat ttttgacaga aagatgatgg aagaaaaaaa ttactaatac
     2281 tgtcatcttg tcattttctg agaatagtta caaaaataaa gaagcttgta aaaatcgaat
     2341 aaaatcaggg aaggatttga agaacaaaga tttgcataaa tagattttcg aaaaattcga
     2401 tatagaaaag aaaagaattc gatgagcgac aattttttca atgtcagaaa cgaattcagc
     2461 aaactggact ctttggccag aaaaggatag cgaccggttt tatacaaata ataaagattt
     2521 cgaattcctt cttcagtttt aaacaaaaag gttggattgt catccaatcc tttatgaatc
     2581 aacggattgt caatatgaaa aacattaaaa cccaattcat gaatccgatg tccgaaaagc
     2641 gtgtcttcgt gcccgtagcc ggaaatggtt tcatcaaagc gaattctgtt gaaaattgat
     2701 tttgcaatta aaaaattaaa tgccgaaaaa tttgatttcc cgctcaattc gagtttgttg
     2761 cgtaaagttg ccgggcgggc ttcgcgcaat ctgccatact ttaatcgcaa actataggca
     2821 aaatcattaa aattttcgtc ataggctgta ccacccacca ccacagactc ctctcgacaa
     2881 aaggaaacgt atttttcgac aaaataggcg gaacaaactt cggcatcaca atcgagaaaa
     2941 agcaaatgcc catattttga catgtcagcc aagcgatttc tgatagctga acggccgata
     3001 tttttttcta aaacgatata actgcaacga ctcaatttat cgatttgcct gttctcttcc
     3061 aagtattcat tagaaccgtc ttccattaca atgatctcaa aatcaataaa aagttggaga
     3121 gcctgccggt gtaattcacc aacaagacgg gaaactgaat aattgaatac cggaatcagg
     3181 attgaaagca ttataaaaga aattgaaaac ttatcagaaa tttttaacgc tcacaaaatt
     3241 aattaaaaat caactgtatt caaaaaattt gattcgataa caaatttttt gacttcgcct
     3301 ctgataaaat ggaatttgaa tatttaacaa aaagcaaaat tgaagttgag gaaggtaaat
     3361 tttaattgtt acgatacctt tctataattt acacaaaagc caatttaaca gaaaaagaaa
     3421 ctgtttgggc acgcttttgc aattttcgag tcatttgttt taatatttct atatcctaat
     3481 aaagtttata tgcagtgatc gggtttttat aaatcactac ggcgtgtctt gccattgtag
     3541 caatgatatc ctcactaaaa aatgacagct tctacattgt ttgggtagtt cagtttcaaa
     3601 agcgaatttt ttacctgttc aagattgaat aacaatgtat gcaatttttg caaactgact
     3661 atgtaagact tagaattgaa gaccgggatg aagtatatag agaagtttta tctcctccga
     3721 cagttaaaat ttagtttatc attaattgtt catcaaaagc atcttcagaa agattataga
     3781 agaattttca aaaaaagcaa cggccagatt aggacaaaca aaaccgattg aataattttg
     3841 aaatgttttt ttcgaaaaat aaagccgact accataccgg ccaacataca acctaataca
     3901 gtaaacattt gaaaatcaat aaaaatcaac agtttcaaac aagaaaaaat ttcccatcaa
     3961 tatgggaata actcagtatt tttcggttaa aaaaagaaaa aatcaaaact aaaaatttac
     4021 ctgcaatgat aatcccattg tatgttgatg attccatgaa aagtcaagtt tatttttgtc
     4081 gatataaggt gtaagttgta atttaacctg ttcctggctg tgcaaatccc tttgatacaa
     4141 attattgatt ttggcaacgt gaaaagcatc aacaacagac cagatatcga ttacaaaaag
     4201 actaacaaca ccgctcagag ctaaaaaggg ataaaaatca gcatccgacc tataataata
     4261 gtcattataa tatccactag agccaagtgc accaatcatg aaaacaaaaa aactcgctgc
     4321 agctccaccc ataaaagcaa aaccccttcc aacttctccg caagttattt gtcccaaacc
     4381 tggaatcaaa taagaagcta tccctgcaac ccaaggctga taaggatctc cgaatttagg
     4441 aaaatacaat tgatctttat agtaattttc tttttccttg taagttttaa aactgatggg
     4501 aggtgtccat gaatcttgag ttaaattacg gatccttccg cgagatactg taatacttcc
     4561 tttctcttct tttatttccg aaacatcagg tagattataa atttgcacca caccgtttga
     4621 aaaatgaatt tcttgtatca tcttaagttc gtacacataa tcgggaccat ccaaatacga
     4681 aaaatcctta cacgtcagtt ctcccgcttt tatttgtgtg attttgcacg ctttgatttc
     4741 tccttttttg aaaacgatgg tatcttgagc cgtcaataga ctaaatgctc cggcccagca
     4801 gaaaagtaca ataaaaaaat gttttttcat gttattgtta tattattaat gatgcaataa
     4861 tttttttata taatttctaa taattaattg cacataatat tttcaaagtc tcattcggct
//
LOCUS       DAOSCH010000131         4842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_11026_length_4842_cov_3.788803, whole genome shotgun sequence.
ACCESSION   DAOSCH010000131 DAOSCH010000000
VERSION     DAOSCH010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11026_length_4842_cov_3.788803"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..557)
                     /locus_tag="PLZ54_08210"
     CDS             complement(126..557)
                     /locus_tag="PLZ54_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66940.1"
                     /translation="MKTKTRKNLAAIARIFSCRVKFLVFAGLICGFSTMVLADHPLPV
                     YHPPRSYPAPVYRYATPYSVTPESSERSFFSVPDYTQRKPFEETDVSMLSSTTKPMRA
                     PAEDDPPFPGDPGQMPVGDGLGFLLLAAAGYALMKRKSFNK"
     gene            complement(1254..>3118)
                     /locus_tag="PLZ54_08215"
     CDS             complement(1254..>3118)
                     /locus_tag="PLZ54_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOJ66941.1"
                     /translation="PEIRIRLAELQARKGNYSQAASLLDGIAGYEERAAFYLNESAQN
                     YIREDSADWLVSPLSFNTSFREYSPALHDRKLFFTANLPLQDKEKSYLWDGRSFSRLW
                     QIPLQELTVEDSTEHFLTGTNKISNVTETKYYAPVYEGSDTRPLWSEVSHKFQTDYVT
                     RTGSPKGSPVFLSEKWKYNVGPASFDSLGNIYFSANSNRFKGKKNTVNILQAKFSNGQ
                     LLDAQAIFPDDHYSYLHPAISADGTLLIFSSNRPGGKGAYDLYYVTRQNLTSGWSEIK
                     SFADSINTPGNEVFPSLSKDGYLYFSSDGKAGFGGLDIYRIPLQEALNGTGKTELIGY
                     PVNSSYDDFGWAQGMDTTRGFFTSDRRGDNDDIFAFRYQPKPKISYITGMVLEKKTRT
                     PMPGATVFLLNEKTGEVLVDKADSTGRYTFVINNMGDYIVKAVEINCKDDCLSMDVSS
                     VKPKYVTFPAPRPLLLELTFKPVWVINNVLYDFDKWHIRDDARPILDSVVTILKTYPI
                     KVELGSHTDCRGSFKYNDRLSQRRAESAVAYIVSQGIDPSRITAKGYGEHKLVNHCAD
                     GVDCTEEEHQQNRRTEITVLYNPSPANSIDPTPYHKGDRLRKENFPENFFTGCR"
     assembly_gap    3119..3218
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3219..3928)
                     /locus_tag="PLZ54_08220"
     CDS             complement(<3219..3928)
                     /locus_tag="PLZ54_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOJ66942.1"
                     /translation="MKNRKYSESFIRNLFLKPAALLVLFSCCHFQISAQQTPMYSQYM
                     FNMLNINPAYAGNRAVNNITALYRDQWVGFEGRPVTAVISWDRRQSESNVGYGLQLYD
                     DRLGIESTTGLQGFYSYRIPFDQNVLTFGLSLGLLNFKAAYTETTIIDASDPAFAQNE
                     SVWLPTAGFGLLFAGENWYGGFSIPALLKTKSFYNDKERTALTAGANYHYFLTGGYIF
                     KASDGIKLKPSLLFKAVSG"
     gene            complement(3957..4607)
                     /locus_tag="PLZ54_08225"
     CDS             complement(3957..4607)
                     /locus_tag="PLZ54_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOJ66943.1"
                     /translation="MFHIIGISTRGGSRSYFKCIGLLWLLMTALPLLAQQRGCTVVIG
                     QPDPMEKPIVEMLSRPDCKNMTGTIQVVSPLGNGYEYSVDGSFYQSSPIFESLNPADY
                     EITVRNANGCISEPATMTIDRFCIPDAFSPNGDEYNQTFEIIHPAEYTINLKIFNRWG
                     NVVFESKNYQNEWDGRGTGAFSGHVLPAGTYYYVAEFLNTFTGVRKTYTGYLTLRR"
BASE COUNT         1442 a          991 c          890 g         1419 t
ORIGIN      
        1 aatcccacgc ataagaattt attgataaac acagaaagct tatcaaaaaa acaatatatt
       61 taatgtgatt tttcatagca aaaaaattaa aagattttta tttattgaaa ttgtcaaacc
      121 attatttact tgttgaatga tttccttttc atcaatgcat atcctgcagc agccaagagc
      181 aggaaaccca gcccatcgcc tacgggcatt tgtccgggat ctccgggaaa gggaggatcg
      241 tcttcagcag gggcacgcat gggcttggta gtggaagaca acatagaaac atccgtttct
      301 tcaaaaggct tgcgctgcgt ataatccggg accgaaaaaa aggagcgttc ggaggactcc
      361 ggtgtaacgg aatagggagt agcgtaccga taaaccggag cagggtaaga acggggcgga
      421 tgatacaccg gcaagggatg atcggctaaa accattgtag aaaaaccaca aatcaaccct
      481 gcaaatacaa ggaacttcac cctgcaagag aatattcttg caattgcagc aagatttttt
      541 cgtgttttcg ttttcatggc catcataggt taaaaatgca atacaaatat aacattatct
      601 taacgaaatt gttctgtttt tttataaaac caaatgacat ttgaatgttt ccgacaattt
      661 tcagagtacc tgaccatcag gcttttctcc ctttccgcaa atcattgctt cgtatattga
      721 catgaacaca gattacgcat tcaaaaatag taaatttcat ttctgcaact caatgaacta
      781 tgttacttaa cgtgagttcg gtataacgat ttacctgttt taataatata aaaagttgat
      841 aattaataag gtaataaggt ttcttcttgt tgttgattat cgttactaag gttttattta
      901 aaaaagtaag gttcattttt ttgaatagaa aaaccttatt tagatacgac taaccttagt
      961 aatatgtgaa tacttccgtt tcatcaacct tattagctta tttataatta cttataaaat
     1021 attcttatat ccaactcaca ttaaaataat aatatataca aatataatcc ataaaacgag
     1081 aaattgttca caattctata taaatttttt attatttctc cgcaaacgat tgaaaaatgt
     1141 taattatttt tcaaattttt ccaatcggtt gataccacac gtcaacaagc acccgaaaca
     1201 gtcgatgaac aaattcatgt ttccttctta aaaacataac ttacaaacgc ttttcaccga
     1261 caaccggtaa agaaattttc ggggaaattt tcctttcgca gcctgtcgcc cttgtggtag
     1321 ggtgtgggat cgatactgtt ggcaggtgaa ggattgtaaa gcaccgtaat ttctgttctt
     1381 ctgttttgct gatgctcttc ttccgtacaa tccactccat ccgcacaatg gttgacaagt
     1441 ttatgctctc cgtaaccttt agcagtgata cgggaagggt cgataccctg actaacgata
     1501 tacgctactg ccgattcggc ccgacgctgc gaaagtcggt cgttgtactt gaacgaccca
     1561 cgacaatcgg tatgagagcc caattctact ttgatgggat aagtttttaa gatagttacc
     1621 acactgtcga gaataggacg tgcatcgtcg cgaatatgcc acttgtcgaa atcatacaac
     1681 acattgttga tcacccacac cggtttgaaa gtcaactcaa gcaacaaagg acgcggagca
     1741 gggaaagtaa catatttcgg ttttactgac gacacatcca tcgacaagca atcgtccttg
     1801 caatttatct caactgcttt tactatatag tcgcccatgt tgttaataac aaaagtgtat
     1861 cttcctgtag aatcggcttt atccaccagc acttctcctg ttttttcatt caataaaaaa
     1921 acggtagcac cgggcatcgg ggtgcgtgtt ttcttttcca gtaccatacc cgtaatatag
     1981 gaaattttag gtttaggctg ataccgaaaa gcaaaaatat catcattgtc ccctcgtctg
     2041 tcggaagtaa aaaaacctcg tgttgtatcc attccttgtg cccaaccaaa atcgtcataa
     2101 gagctgttga cgggataccc aattaattca gttttacctg ttccattcaa tgcttcctgt
     2161 aaagggatgc gataaatatc caacccgcca aaaccagctt ttccgtcgct gctgaaataa
     2221 agataaccat ctttactcaa ggaaggaaaa acttcgttac ccggagtatt aatggaatcg
     2281 gcaaatgatt ttatttcact ccaaccggag gttaaattct ggcgtgttac ataatataaa
     2341 tcataagccc cttttcctcc cggtcgattg ctgctaaaaa tcaacaaagt tccatccgct
     2401 gaaatggccg gatgaagata ggaataatga tcatcaggga aaatagcctg tgcatctaac
     2461 aattgaccat tggaaaattt agcttgcaga atgttgactg tatttttttt acccttgaaa
     2521 cgattggaat tggctgaaaa ataaatatta cctaaggaat cgaaacttgc cggtccgaca
     2581 ttgtatttcc atttctcaga taaaaaaaca ggacttcctt taggtgagcc ggtacgtgtt
     2641 acataatcgg tttgaaattt gtgtgaaact tcgctccaca atggccgtgt atcacttcct
     2701 tcataaacgg gggcataata tttggtttcc gtaacattag aaattttatt ggtacctgtc
     2761 agaaaatgct ctgtagaatc ttccactgtc agctcctgca agggaatttg ccacagtcgc
     2821 gaaaaacttc tgccatccca taaataagac ttctccttgt cctgaagcgg cagatttgcc
     2881 gtaaaaaaca attttctatc gtgtaatgcg ggcgaatatt ctctgaatga tgtgttgaag
     2941 ctcaaaggtg aaaccaacca atcggcggaa tcctctctga tatagttttg agcagattca
     3001 ttgagataaa atgccgctct ttcttcatac cctgcaattc cgtccaacaa ggatgcagct
     3061 tgggaataat ttccttttcg tgcctgcaat tcagccaagc gaattcttat ttcgggaann
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc ggaaacggct ttaaacaaaa
     3241 gagaaggttt gagttttatt ccgtccgaag ctttaaaaat atatcctccg gtaagaaagt
     3301 aatgataatt ggctccggcg gtcaaagcgg ttcgttcttt gtcgttgtag aaacttttgg
     3361 tcttcagcag tgcggggatg gaaaacccac cataccaatt ttctccggca aacagcaaac
     3421 caaagccggc agtgggtagc caaacgcttt cgttctgtgc aaaagcaggg tcgcttgcat
     3481 cgataatagt agtttcggtg taggctgcct taaaatttaa taatcccaga ctcaaaccaa
     3541 aagtcaacac attttgatca aatggaatgc ggtaagaata aaatccttgt aaaccggtgg
     3601 tgctttcaat acccaatcgg tcgtcgtaaa gttgcagccc atatcccaca ttgctttccg
     3661 attgcctgcg atcccatgag ataacagccg ttaccggtct tccttcaaat cctacccact
     3721 gatctctgta taaggcagtg atattgttta cagccctatt gccggcataa gcggggttga
     3781 tattcagcat gttgaacata tactgcgaat acatgggcgt ctgctgggcg gatatttgaa
     3841 aatggcagca cgagaaaagc accaacaatg ctgccggttt caagaataaa tttcggataa
     3901 aactttcaga atatttccta tttttcatgg atacgttctc cttttttgat gaactcttat
     3961 cgccgaagcg tcaaatatcc ggtataggtt tttcgaacgc cggtaaaggt attcaaaaac
     4021 tcggcaacat aataataggt tccagccggc aacacatgac cggaaaatgc acctgttccc
     4081 ctaccatccc attcgttttg ataatttttg ctctcaaaca ccacatttcc ccaacgattg
     4141 aaaattttca agttaattgt atattcggcc ggatggataa tctcgaaggt ttggttgtat
     4201 tcgtcaccat tcggtgaaaa agcatccggt atacaaaagc ggtcgatggt cattgttgca
     4261 ggttcggaaa tacaaccatt ggcattcctg accgtaattt cataatcggc cggattcaaa
     4321 gactcaaaaa tcggactgct ttggtaaaaa gaaccatcca cagagtattc atatccattt
     4381 ccgagcggag aaaccacctg aatagtgccg gtcatatttt tgcaatcggg tcttgaaagc
     4441 atttcgacaa tgggtttttc catgggatca ggttgcccaa tgacaacagt gcaaccccgc
     4501 tgctgagcca acaaaggcag cgctgtcatc agtagccaca acagtccgat acatttgaaa
     4561 taactacgac ttcccccccg agttgatatg cctatgatat gaaacatgag tttcaatttt
     4621 gtttttgtga atttacatta acgtgagttc gatatgacga ttacacattt ttaatcattt
     4681 aaaaaattga taattaataa ggtaataagg tttttttctt gttattgatt atcgtttgca
     4741 tacttcattt taatccacct tattcgctta tttataattg cttatataat tcttatatcg
     4801 gactcacgtt acattaagtt caatccgtcc ccttatttaa ga
//
LOCUS       DAOSCH010000132         4680 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_11565_length_4680_cov_8.842595, whole genome shotgun sequence.
ACCESSION   DAOSCH010000132 DAOSCH010000000
VERSION     DAOSCH010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4680
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11565_length_4680_cov_8.842595"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="PLZ54_08230"
     CDS             complement(<1..581)
                     /locus_tag="PLZ54_08230"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107447.1"
                     /note="catalyzes the interconversion of D-xylose to
                     D-xylulose; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HOJ66944.1"
                     /translation="MTIKEYFPTVGKIKFEGKESKNPFSFRYYDAEKVVYGRKMKDWF
                     KFSMAYWHTLCAEGSDQFGGPTKSFPWNEGKVALERAKNKMDAGFEFMQKVGINYYCF
                     HDIDLIDEGNSIEEYEANMKAIVEYAKQKQAETGIKLLWGTANVFSHKRYMNGAATNP
                     DFNAVARAAVQIKNALDATIALGGENYVFWGGR"
     gene            complement(673..2151)
                     /locus_tag="PLZ54_08235"
     CDS             complement(673..2151)
                     /locus_tag="PLZ54_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HOJ66945.1"
                     /translation="MYLLGYDIGSSSIKASLINATTGECVSSEFFPKKEMQIKSVKPG
                     WAEQDPETWWENLKLATQAVITDSKVRSEEIKAIGISYQMHGLVLVDERLQVIRDAII
                     WCDSRAVPYGEKAFKEIGEEKCLSRLLNSPGNFTLAKLAWVKENEPENFKRIKYYMLP
                     GDYIALRLTGKPCTTASGLSEGIAWDFTKEAPADFLFDYFGFSTSIIPEIVPTFGEQG
                     FLSAEAAHELELAAGTAVTYRAGDQPNNALSLKVLNPGEIAATAGTSGVVYGVNGEIK
                     YDPQSRVNSFAHVNHSAGEKRIGVLLCINGTAILNAWIKNNVAPEGVSYNEMNEMAAK
                     IPIGSGGVSIIPFGNGAERMLGNREPNCSIHGINFNHIGKAHLIRAAHEGIAFSFKYG
                     MDIMNEIGIETKTIRAGHANLFLSPVFRQTLANITGATIELYNTDGSIGAARGAGIGA
                     EIYKNEEEAFATLKKILTVQPEKENQEITREAYDRWLQHLKF"
     gene            complement(2196..2906)
                     /locus_tag="PLZ54_08240"
     CDS             complement(2196..2906)
                     /locus_tag="PLZ54_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ66946.1"
                     /translation="MESVNVYNPHVSVDCVVFGFDGEKLKVLLIERTIDEPGEQYNDK
                     KLPGSIIYENENLDEAASRVLGELTGLKNIYLSQFKSFGDPKRTSNPRDIYWLENTTK
                     MKIGRIVTVGYVALIKINRKIKLEEDNKTVQWYDVKDIDKINLAFDHNEIIHAALEHI
                     RHTLKVEPRLMFELLPRKFTMSQLRILYDVIHQTHSDVRNFQKKMMQLHQLVMLDEVE
                     KNVPHRAARLYKFDRKKL"
     gene            complement(3498..4442)
                     /locus_tag="PLZ54_08245"
     CDS             complement(3498..4442)
                     /locus_tag="PLZ54_08245"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HOJ66947.1"
                     /translation="MYKIIVVLVGFMSFFSSVQGQLLWKISGNGLKKSSYLFGTHHFI
                     PLQFLDSIPGLYQAFNTSEIVVGEMVVNNLDAVEKVKKAAIIPNQQTIYDLVDKNQIL
                     QIDSALKSVLHIGLKEMALLQPNLILTIYQTEVYKKASGQIENIAPDSYFQQLGIAKG
                     KKIVGLETIDQQLEILFDKTSLQRQTEIFTYSVLHTDNMILETNKLNNFYKKGDLEKL
                     LLLSIQKNNPSDATAEEYYKLVDERNLEWMKQLPELIKSSSCFITVGALHLPGENGLI
                     EQFRKLGYHVTEVKKLGNNYIYYLKLLHNQLSKGKSSR"
BASE COUNT         1396 a          931 c          760 g         1593 t
ORIGIN      
        1 tcacgtccac cccagaatac ataattttcg ccaccaagag caatcgtggc atccagcgca
       61 ttcttgatct gaacggctgc ccgtgctaca gcattaaaat caggattggt agcagctccg
      121 ttcatatatc gtttgtgcga aaatacgttg gctgttcccc aaagcaactt gatccccgtt
      181 tcagcctgtt tttgcttcgc atattccaca atggctttca tattggcttc atattcttca
      241 atggaatttc cttcatcaat caaatcgata tcatggaaac aataataatt gataccaacc
      301 ttttgcatga attcaaaacc agcatccatt ttgtttttgg ctctttccaa tgcaactttt
      361 ccttcattcc acggaaaact tttggtgggt ccgccaaact ggtcgcttcc ttctgcacac
      421 aatgtatgcc agtatgccat tgaaaattta aaccaatctt tcattttacg gccatacact
      481 actttttcgg catcgtagta ccgaaacgaa aaaggatttt tactctcttt tccttcaaat
      541 ttgatttttc ccactgttgg aaaatactct tttattgtca taattcataa attattaaat
      601 tcctcagcca aattagctta agaataagtt aaacattttt tattatcttg taattatcac
      661 taaaatttct atttaaaatt tcaaatgctg caaccatcga tcgtaagctt cacgcgtaat
      721 ttcctgattc tctttttcag gctgaacggt aagaattttc tttaacgttg caaaagcttc
      781 ttcttcgttt ttataaattt ccgctcctat tcctgctcca cgagcagcac ctatcgagcc
      841 atcagtatta tacaactcaa tggttgcacc ggttatgtta gccagtgtct gacggaaaac
      901 gggcgaaagg aaaaggttgg catgtccggc acgaatggtt ttcgtctcga tacctatttc
      961 attcataata tccattccgt atttaaacga aaatgcaatg ccttcatgag cagctcttat
     1021 taaatgagct ttgccgatat gattgaaatt gatgccatga atggaacaat tgggttcgcg
     1081 atttcccaac atccgttctg ctccattacc aaaaggaata atacttacgc cgccactgcc
     1141 aatagggatt tttgctgcca tttcattcat ttcattataa ctcacacctt ccggagccac
     1201 gttatttttt atccatgcat tcaatatagc tgtaccatta atacaaagca gcacaccaat
     1261 acgcttttca ccggcagaat ggttgacatg ggcaaacgaa ttgactctcg actgcggatc
     1321 gtattttatt tctccgttaa caccataaac aacgcctgaa gttccggcag tagcagcgat
     1381 ttcaccagga tttaatacct ttaacgacaa agcattgttt ggttgatcgc cggcacgata
     1441 agtaacggct gttcctgctg caagttcaag ttcatgcgca gcttctgcac tgaggaatcc
     1501 ctgctcacca aaagttggaa caatttcagg aataatggaa gtactaaacc caaaataatc
     1561 aaacagaaaa tcggcaggag cttcctttgt gaaatcccat gctattcctt ccgacaagcc
     1621 cgatgcagta gtacagggct ttcctgtcag tcgcaaagca atgtaatcgc ccggcaacat
     1681 gtaatatttg attcgtttga aattttccgg ttcattttct tttacccagg ccagttttgc
     1741 aagcgtaaaa tttcccggag aattcaataa gcgagataag catttttctt ctccaatttc
     1801 cttgaacgct ttttctccgt aaggtacagc acgactgtcg caccagataa ttgcatcccg
     1861 aattacttgc agtcgttcgt ccaccaatac caaaccatgc atctgatagg aaatgccgat
     1921 ggctttaatt tcttccgacc gaactttact gtctgttatt acagcttgcg ttgcaagttt
     1981 cagattttcc caccacgttt ccgggtcttg ttctgcccat ccgggtttca ccgatttaat
     2041 ctgcatttct ttcttcggga aaaattcact cgaaacacat tcgcctgttg tggcattaat
     2101 aagacttgcc ttaatggagg aacttcctat atcatatcca agtaaataca taattcttag
     2161 tcgattattg atttataata atgatttttt ttggtttata atttctttct atcaaattta
     2221 tacaaccgtg cagcacgatg aggcacattt ttttctactt cgtcgagcat gaccagttga
     2281 tgcaactgca tcattttttt ctgaaagttt cttacatccg aatgagtttg atgaataaca
     2341 tcatagagaa ttcttaattg tgacatggta aattttcggg gcagcaattc aaacataaga
     2401 cgaggctcta ctttcaaagt atgccgaata tgttccaaag ccgcatgaat gatttcattg
     2461 tggtcgaaag caagattaat tttgtcgata tctttcacat cgtaccactg caccgttttg
     2521 ttatcttctt ccagcttaat ttttctgttt attttgatta aagccacata gccaaccgtt
     2581 acaatacgcc cgattttcat tttcgtggta ttttcaagcc aataaatatc gcgtggatta
     2641 ctggttctct tgggatcacc aaaactctta aactgactga gataaatatt cttcaatccc
     2701 gtgagttctc ctaaaacacg actggcagct tcgtccaaat tttcattctc gtaaataatg
     2761 ctccccggca gtttcttatc attgtattgc tcaccgggtt cgtcgatggt tcgctcaata
     2821 agcaaaactt tcagtttctc tccatcaaat ccgaaaacca cacagtcaac cgagacatgc
     2881 ggattataaa catttactga ttccattgat ttataaagaa gtttttgaat aacgacacaa
     2941 aaataatatt caaatataaa aatatctaat taaatattat gttataaaac acatttacaa
     3001 tatattaaac attgtatttt atacaatatg taattgaatc atattgtata aagtacaata
     3061 ataccaatga aaacaacaaa atttatttat tgaaaaaaag gcacagcaaa atatttcgtc
     3121 tgtttactta aaagattttc agctttttac aatgaaataa taaaactttt ttcttttttg
     3181 ggagaaatgc caaataactt ttgggaatag aaaaattttt tctgaaaaaa caataaaaag
     3241 aaagagtctt ctcttaaaaa cctaattttt ttaaagtttc attcggcttt aatgtagtag
     3301 acataagctg ccaagacttg gcaatgcgtt ctttgataga tgataaatat cgattgtgag
     3361 ttattgactt tcgcatatac caccaaacac gttcaacagg attcaagtcc ggagaatatg
     3421 ccggaagata tcttatttcc aatgtgggat tagcttccag aaagccctta agcttttttg
     3481 catgatgaaa cctcacgtta tctactggat ttacctttgg acaactgatt gtgtaataat
     3541 ttaaggtaat atatataatt gttgcctaat ttttttacct ctgttacgtg atatcccaat
     3601 tttctgaatt gttctatcag gccgttttcg ccaggcaaat gcagtgctcc gactgtaata
     3661 aaacatgatg agctttttat cagttcaggt agctgtttca tccattcaag atttctttca
     3721 tccactaatt tataatattc ttcagctgta gcatctgaag gattgttttt ttgaatcgac
     3781 aataaaagca atttttctaa atcacccttt ttgtaaaaat tattcaattt gttggtttct
     3841 aaaatcatgt tgtcggtatg aagaactgaa taagtaaata tttctgtctg acgttgcagg
     3901 gaagttttgt caaaaagtat ttccagttgc tggtcgatgg tttcgagtcc tacaattttt
     3961 tttccttttg ctattcctaa ctgttgaaaa tacgaatcgg gtgcaatatt ttcaatttgg
     4021 ccggaggctt ttttgtatac ttcggtttga taaatggtta aaatcaaatt gggctgcagc
     4081 agggccattt ctttcaatcc tatgtgaaga accgatttta gagctgagtc gatttgcaag
     4141 atttgatttt tatctactaa gtcgtaaata gtttgctggt tgggaataat agctgctttc
     4201 ttaacttttt ccaccgcatc caggttgttc accaccattt ctccaaccac aatttcagaa
     4261 gtattaaatg cttggtataa gccagggata ctatcgagaa attgtaacgg aataaaatga
     4321 tgggtgccaa acaaataaga actttttttc aatccgttgc ctgagatttt ccataacaac
     4381 tgtccttgaa cggatgaaaa aaacgacatg aatcctacca gaacaacaat tattttatac
     4441 atgaaatgtt aaattatatg gttggcacaa atatatgcaa attatttgtt gagataagga
     4501 aacaaaatca ggcaactact gttttttgtg ttttttaatt gaaattacga tttctatcaa
     4561 ttcacatttg tgttttgtaa catttttgaa atatcttttt aatggatttg aaatttgtgt
     4621 tataaaaaac gatagttatc tttgtgcaaa attattaatt tctaatatga gtaacatata
//
LOCUS       DAOSCH010000133         4631 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_11730_length_4631_cov_7.233610, whole genome shotgun sequence.
ACCESSION   DAOSCH010000133 DAOSCH010000000
VERSION     DAOSCH010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11730_length_4631_cov_7.233610"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /gene="leuB"
                     /locus_tag="PLZ54_08250"
     CDS             <1..893
                     /gene="leuB"
                     /locus_tag="PLZ54_08250"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HOJ66948.1"
                     /translation="LCMQSDAVLFGAIGSPKYDNNPSAKVRPEQGLLKMRKELGLYAN
                     IRPVTTFASLIKKSPLKTSLIEGADFICIRELTGGIYFGRPQGRSEDGNTAYDTCIYT
                     REEIERVVELAYKFASQRRKKITMVDKANVLATSRLWRQITQEISAKYPQIDTEYLFV
                     DNAAMRIIQNPKNFDVIVTENLFGDILTDEASVITGSLGMLPSASMGIHTSLFEPVHG
                     SYPQATGKNIANPMATILSAALMFEYAFNLKEESNLIREAVDACITAGVVTEDIANGQ
                     KAYKTTEVGQWIVNYIDNKK"
     gene            complement(927..1544)
                     /locus_tag="PLZ54_08255"
     CDS             complement(927..1544)
                     /locus_tag="PLZ54_08255"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOJ66949.1"
                     /translation="MAGRLQAQIICFLLLSCFQFLPESCHSPTKIENSDTYITNYLRS
                     GDIILRKSYGLVSNIIAAKLNDTIDVSHCGIIYIDANHTVYVIHSLSKAVSEKDGMQS
                     CTLKKFMADSRIETVQVVRFKKDSAQIMGKWAHYYLLKQIPFDNNYDLKDSSSFFCTE
                     LPIHIINKQFGINLCSGNELPKFSIFLNPKYFDKVFFIKKANLNH"
     gene            complement(1517..1894)
                     /locus_tag="PLZ54_08260"
     CDS             complement(1517..1894)
                     /locus_tag="PLZ54_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HOJ66950.1"
                     /translation="MAVIFLALGACSSNSNSPEAVAEKFNKAIYTADFNAAKALCTDD
                     SKQAVDLIASFVSEKAGDMKKANVKYETKSVNLSEDGNSADVEGVVLNAYDLQNDKIA
                     DSTDTKVHLVKENGKWLVDYKLK"
     gene            complement(2118..4313)
                     /gene="rnr"
                     /locus_tag="PLZ54_08265"
     CDS             complement(2118..4313)
                     /gene="rnr"
                     /locus_tag="PLZ54_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOJ66951.1"
                     /translation="MSKKTNRNTSTRIPRKQLINAISDIFNQHPRETYNYRQIAKMLN
                     IENQSQQQYIRQLLEGLADEEFVTEISKGRFKANFPNGNSIIGTIDRQGVKTYVIPDD
                     GGEMIFIPERKTNHALLNDKVKVVLYARRKGQQQEGEVVEVLQRAQDTFVGTLQVSEN
                     FAFLVVDNRIMTSDIFIPRNKLNGGKNGQKAVVKLMEWQPDLKSPVGEVIDVLGDKGN
                     NNTEMHAILAEFGLPYRYPVEEEKAAEKIDDGITPEEIAKRRDMRKVTTFTIDPRNAK
                     DFDDALSIRKLNNGLWEIGVHIADVTHYVQPRTIIDREAEKRATSIYLVDRTIPMLPE
                     HLSNGICSLRPNEDKLTFSVVFQIDENADVKGYEIVHTVINSNRRFTYEEVQEIIESG
                     KGDYSAEILQLNKMAQILRKRRFENGAIAFERTEVQFEIDKKGRPISVFFKEQKESHQ
                     LIEEFMLLANRTVATHIGKPAKGSKAKTFVYRVHEVPSPDKLRDLSAFVKKFGYHFKT
                     SGKQTSVSKGINKLLDEVQGKKEQNLIETLAIRSMSKAVYSTNNVGHYGLAFDYYTHF
                     TSPIRRYPDMMVHRLLDRYLANGKSVNAEEWEEYCLHSSDMEQLAAKAERTSIKYKQV
                     EFMSERIGQIYDGIISGITEWGIYVEIIENKCEGMIPIRDLDDDYYIFDEKNYCLTGK
                     RYHKRYRLGDEIKIRVAKANLDKRELDFALAGNFGKMTQKKNHSGEKDF"
BASE COUNT         1362 a          835 c          870 g         1564 t
ORIGIN      
        1 aattgtgcat gcaatctgat gcggtacttt ttggagcaat tggaagtcct aaatacgaca
       61 ataatccatc agcaaaagtt cgtcctgaac aaggtttact taaaatgcga aaagaattag
      121 gtttatatgc caacattcgt ccagtaacaa cctttgcttc attaatcaaa aaatcaccgt
      181 tgaaaacttc gttgatagaa ggagctgatt ttatttgtat tagagaactg acgggtggaa
      241 tatatttcgg gcgtcctcaa ggaagaagtg aggatggaaa tacagcttac gatacttgta
      301 tttatactcg cgaagaaatt gaaagagtgg tggaattggc ttataaattt gccagtcaac
      361 gtagaaaaaa aataacaatg gttgataagg caaatgtttt ggctacttct cgtttatgga
      421 gacagattac tcaagaaatt tcagcaaaat atccacaaat agatacagaa tacctttttg
      481 tggacaatgc tgcgatgcgt attatacaaa atcccaaaaa ttttgatgtc attgtaactg
      541 aaaatctttt cggcgacatt ctcacagatg aagcttcggt aattacaggt tcattaggaa
      601 tgttaccttc tgcttcgatg ggtattcata cttcactttt tgaacctgta catggttctt
      661 atccgcaagc tacggggaaa aacatagcca atccaatggc aactatttta tcagcagctt
      721 tgatgtttga atatgctttt aatttaaagg aagaaagtaa tttaattcgt gaagctgtcg
      781 atgcttgtat cacagcgggc gtagtaaccg aagatattgc caacggtcaa aaagcataca
      841 aaactactga ggttggacaa tggatagtta attacatcga taataaaaaa taaaataaga
      901 tttataatct ttgcttcagg cggactttaa tgattaaggt tcgctttttt tataaaaaag
      961 actttatcga aatacttggg atttagaaat atagagaatt ttggaagctc attgcccgaa
     1021 caaagattga tcccgaattg tttgtttatg atgtgaatgg gaagttctgt acagaaaaat
     1081 gaagaagaat cttttaaatc gtaattattg tcaaaaggga tttgttttaa aagataatag
     1141 tgagcccatt tgcccataat ttgagcggaa tcttttttga acctgacaac ctgtaccgtt
     1201 tcaatacgac tgtcggccat aaatttttta agcgtgcaac tttgcatgcc atctttttcc
     1261 gaaactgctt ttgaaaggga atgaataacg tacacggtat gattggcatc aatataaata
     1321 atgccacaat gcgaaacgtc aatcgtatcg ttcaatttag ccgctattat gttgctgaca
     1381 agcccgtaac tttttcgtaa aattatgtcg ccgctacgaa gataattggt aatataagta
     1441 tctgaatttt caatttttgt cggagaatgg caactctccg gcaaaaattg aaaacacgac
     1501 aacaaaagaa agcaaattat ttgagcttgt aatctaccag ccacttgccg ttttctttaa
     1561 ccaaatgaac ttttgtatcc gttgaatctg ctattttatc attctgcaaa tcgtaagcgt
     1621 ttaacacaac accctcaaca tctgccgaat ttccatcttc ggataaattg accgattttg
     1681 tttcgtattt aacatttgct tttttcatat cacctgcttt ttcggaaaca aaagaagcaa
     1741 ttaaatctac tgcctgcttg ctgtcgtcgg tacataaggc tttggctgca ttgaaatcgg
     1801 ccgtataaat agccttgtta aatttttctg ctactgcttc aggagaatta ctattggaag
     1861 aacaagctcc aagtgccaga aaaatgactg ccaaaaaaaa taaattcaaa tttttcataa
     1921 tcttttaaat tttagttgaa ttaattaccg aattttaatt gtcggctgtt taaaggcgta
     1981 aaagtataaa ataaatttac gaaaaacaaa caaaaactaa aattaattca aaataattat
     2041 aaaaaatgag aacttctaaa aataggaatt acaagtataa atatcgacat aattaaaagt
     2101 ttgtcggata aacttgatta aaaatctttt tctcctgaat gatttttctt ttgtgtcatt
     2161 tttccaaagt taccagccaa tgcaaaatcg agctctcttt tgtcgagatt ggctttggca
     2221 actcgaattt taatttcgtc tcccaagcgg tagcgtttat ggtagcgttt tccggtaaga
     2281 cagtagtttt tttcgtcaaa aatgtaataa tcgtcgtcca aatcgcgaat aggaatcatt
     2341 ccttcacatt tgttttcaat tatttctacg taaattcccc attcggttat gccggatatg
     2401 atcccatcgt agatttgtcc gattctttca gacataaatt ccacctgttt gtatttgatg
     2461 gaagtgcgtt cggctttggc tgccaattgc tccatgtctg agctgtggag gcaatattct
     2521 tcccattctt ctgcattgac acttttcccg ttggccaaat atctgtcgag cagacgatgc
     2581 accatcatgt cgggataacg gcgaatggga gaagtgaaat gggtatagta atcgaaagca
     2641 aggccataat gaccgacatt gttggttgaa taaactgctt tggacatgga gcggatagcc
     2701 agcgtttcta tcagattttg ttcttttttc ccctgaactt catccaacaa tttgttgatt
     2761 ccctttgaaa ctgaggtttg tttaccggaa gttttgaaat gatagccaaa ttttttaaca
     2821 aaagcagaca aatcgcgtaa tttatcaggg cttggaactt catggacacg atagacaaag
     2881 gttttagctt tactaccttt tgccggcttg ccaatgtgtg tggctacagt acggttggcc
     2941 agcagcataa attcttcgat caattgatgg gattcctttt gctctttaaa aaaaaccgaa
     3001 atgggtcgtc cttttttgtc gatttcaaat tgaacttcgg tacgttcaaa agcaatggct
     3061 ccgttctcga aacgtctttt acgaagaatt tgtgccattt tgttcaactg aagaatttct
     3121 gctgaataat caccttttcc actttcgatg atttcctgaa cttcctcgta ggtaaatcga
     3181 cgattactat tgattacggt atgaacaatt tcgtagcctt ttacatctgc attttcatca
     3241 atttgaaaaa caacggaaaa agtcagtttg tcttcattgg gtcgaagcga acaaattccg
     3301 ttagataagt gttcaggcaa cataggtatc gttctgtcca ccaaatagat agaagtggct
     3361 cttttttctg cttcacggtc aataatggtt ctgggctgaa cgtaatgagt tacatcggcg
     3421 atgtgaacgc caatttccca aagtccatta ttgagttttc tgatggaaag tgcatcatca
     3481 aagtctttgg cattgcgcgg atcgatggta aaagtggtta cctttctcat gtcgcgtctt
     3541 tttgctattt cttcgggtgt aattccgtcg tcaatttttt cggctgcttt ttcttcttca
     3601 accggatatc tgtacggcaa cccaaattcg gccagaatgg cgtgcatttc ggtattatta
     3661 ttgcctttat ctcccaagac atctattact tcgcctacgg gacttttcag atcgggttgc
     3721 cattccatca gttttaccac agctttttga ccgttttttc caccgttgag tttgtttctg
     3781 gggataaaaa tatcactggt catgatgcgg ttgtccacca ccagaaaggc aaaattttca
     3841 gaaacctgta aagtaccgac aaacgtatcc tgtgcgcgtt gcaaaacttc caccacttct
     3901 ccttcctgct gttggccttt tcgccgagca tacagaacaa cttttacctt atcgttaagc
     3961 agggcatggt tggttttgcg ttcgggtata aaaatcattt ctccgccatc gtcgggaatc
     4021 acataagttt taacgccttg tcggtcaatg gttccgatta tgctgtttcc attcgggaaa
     4081 ttggctttga aacgtccttt cgaaatttcc gtaacaaatt cttcatctgc caatccctcg
     4141 agcagttgtc ggatgtattg ttgctgcgat tgattttcaa tattcagcat tttggctatt
     4201 tgtcggtaat tgtatgtttc gcgcggatgc tggttgaaaa tatcgctgat ggcgtttatc
     4261 aattgtttac gcggaattcg cgtgctggta tttctatttg tttttttcga catgtttatt
     4321 tgttttttga atggaagatt ttaaaaattt tccattattt tttttgaatt aatgtttgat
     4381 tatcaataaa tttttgctgt attaaaaagt ctccggatag tatttttgcg gtcaggagaa
     4441 ataaaaatgc aaagttacga ctttttcttc aaaaaatttt gatggttacg ttcaacttag
     4501 cgtccgaatt gagcagaaag tattaaattt tggtttttga atatttcaat tttattacat
     4561 tgtttataac ttttcgttgc tgaattcaag tcacaaatgc aactttttgg ttatacaaat
     4621 ttcgtaataa a
//
LOCUS       DAOSCH010000134         4543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_12031_length_4543_cov_5.727050, whole genome shotgun sequence.
ACCESSION   DAOSCH010000134 DAOSCH010000000
VERSION     DAOSCH010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12031_length_4543_cov_5.727050"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            390..1157
                     /locus_tag="PLZ54_08270"
     CDS             390..1157
                     /locus_tag="PLZ54_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66952.1"
                     /translation="MEVFAAPSRWGGIVAGSPKVPSLFTIRNPQTWVLRKDGTAVDSS
                     VSGRISSINSEETYRFVESLLDKLFRKWNFKGVIWDEPKTLSLDYSPAAIQKFGNNPT
                     MDQQIQANVDFYSRLNKYVKTKYPDKTTCLFIYSSYEDEIINKMAAIEYLDYFGADGR
                     PWHSADGGQLEGNGKTLLGTQGQRFIDAAHANNKKSLFLMENHNLKKADIHLLEKGMP
                     QLLQMDVDQLIYYYYPRNVEEPDKTMEIIKWNLKKYR"
     gene            1228..4335
                     /locus_tag="PLZ54_08275"
     CDS             1228..4335
                     /locus_tag="PLZ54_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HOJ66953.1"
                     /translation="MTLKKIQIAIFLCISSSFSLFAQTPDWENPRVFGVNNEPTRASF
                     LPYQSESMAIEDDYLRSSYYMLLDGNWKFNFSPRPDLRPVDFYNQEYNVSGWKEIKVP
                     GNWELQGYGKPIYTNIIYPHPKNPPYIPHTDNPVGSYRRDFHLPESWDGRRVFLHFEN
                     GTAAMYVWINGKKVGYNQGAKSPVEFDITNYVQKGKNSIACEVYRWSDGSYLEDQDFW
                     RLSGFERSIYLYSTDQSRIFDFFVKTDLDKAYKNAMLSLDIKLKNYTNTKKKQIVEIK
                     LLDSNMKAVFTQQKVVQIEGENSVESSFVQKVSAPKLWTAETPNLYTLVLTLKNENGK
                     AIEFTSTKVGFRKIEIKDGQLLVNGKRVLFKGVNIHEHNHLTGHTINREIMMQDIRLM
                     KQFNINAVRTSHYPQPVLWYKLCDEYGIYLIDEANIESHGMGYGKESITHFPEWWDAH
                     LDRTIRLVERDKNHPSVIVWSLGNESANGKAFEITYDWVKNRDNTRPVQYEQAHEERN
                     TDIICPMYPDFEHMRKFASRKLDRPYIMCEYAHAMGNSIGNFQEYWDIIRSSKHFQGG
                     YIWDWVDQGLLQKDENGREYWAYGGDFGVGFEYHNDANFCANGLVAPDRTPHPHLYEV
                     KKVYQNVLINAKDLSKGIVTVTNDYRFTNLKDILYFEWHLLKNGVKVSQGKFDVDLAP
                     EASKDVRLALPELKKQQGDEYFLQIYGYTLNKTELIPANFELVREEFALSGNDYFTLT
                     KLSTSKPQFVAKDNVIHVTAGDLLAEFHKSVAGLVELSYKGIQLLKGKSPELNFWRAP
                     TDNDWGEGAHKKMNIWRNAGNSVVLKDVVLVEGENKLQINYHLFSTDIQSDIFINYTV
                     SGDGSIVCDIEYKTQNNFLPEMPRFGIMFTLPEEFENLSWYGRGPWENYIDRNVSSFM
                     GIYESKVKDQYYPYVRPQENGNKTDIRWITLTNNEGVGLKVEGLQPISFSALNYRPED
                     FDPGFSKKQQHISDMYPRREVVLSVDLFQRGLGGLNSWGAGPLKQYRFTEKIYSYSFK
                     ISGVE"
BASE COUNT         1453 a          780 c          992 g         1318 t
ORIGIN      
        1 acaaacacat gaacttcgag ttcgcggatg aaattttaac tccttatctg ttttttcaat
       61 atgctgatat tgtttaattt aataattatt tagggatgaa aaagaattac ttagtcttac
      121 tggcattact ttttataggt ggtttcatta gattacttgc tgaaaacccc gaatacgtgc
      181 tgcagaaaca acagccgggt aaaaagtacc tgaatgcatt ctattacagg gcgcatatgt
      241 acaccatggt tcctcgccag attagggaag atatgaaatg gatggctcgt gccggaacga
      301 atgccatcac gatatcggtg cttgaacagg attttgatgc tgctgatgag aatatacgat
      361 ttattataag tgaggccaac aagcagggca tggaggtatt cgcagcgcct tcacggtggg
      421 gagggatagt tgccggctcg ccaaaggttc ccagtctttt taccattcgt aatccacaga
      481 catgggtttt gcgcaaagat ggtactgcgg tcgactcttc agtttcgggc aggataagca
      541 gtataaacag cgaagaaacc tatcggtttg tcgaaagctt attggataag ttattccgga
      601 aatggaactt taagggcgtg atatgggacg aacccaaaac attgagcctg gactactcgc
      661 ccgctgcaat ccagaagttc gggaataacc cgaccatgga ccagcaaatc caggctaatg
      721 tggattttta ttcccggctg aataagtatg tgaaaaccaa atatcccgat aagaccacct
      781 gtttgttcat ttattccagt tatgaggacg agataatcaa taaaatggcc gcgattgagt
      841 atcttgacta ttttggagcc gatggaaggc cttggcactc ggcggatggc ggtcaactcg
      901 aaggtaatgg gaaaacctta ctgggaacgc agggacaacg ttttatagat gctgcacatg
      961 ccaacaataa aaagtcactt ttcctgatgg agaatcataa tttaaagaaa gcagatattc
     1021 atttgctgga aaaaggaatg ccacagctgc tgcaaatgga tgtggatcaa ctcatctatt
     1081 attattatcc gcggaatgtt gaggagcctg ataaaacaat ggaaataata aaatggaatt
     1141 taaagaagta cagataagga acataaggtc ttagtcagat caatgcaagc ttttaccgga
     1201 caataaaaaa attaaaaatc atataacatg acattaaaaa aaattcagat tgctattttc
     1261 ttgtgcatct caagttcttt ttctcttttt gcacaaaccc ccgattggga aaatccgcga
     1321 gtttttggag tgaataatga acccaccagg gcaagttttc ttccatatca aagcgaaagt
     1381 atggcaattg aagatgacta tttgcgttca tcgtactata tgttgttgga tggcaactgg
     1441 aagttcaact tttcgccacg tcccgacctg cgccctgtcg atttttataa ccaggaatac
     1501 aatgtgtccg gttggaaaga gataaaagtt ccgggaaact gggaattgca agggtatggt
     1561 aaacctattt ataccaacat tatttatccg caccccaaaa atcctccgta tattccgcac
     1621 accgataatc ctgtgggatc ataccgacgg gattttcatt tgcctgaaag ttgggatgga
     1681 cgacgggtct ttctgcattt cgaaaatgga actgcggcaa tgtatgtttg gattaatggc
     1741 aaaaaggtgg gatataatca gggagcaaaa agtccggttg aattcgatat aaccaattat
     1801 gtgcaaaaag gcaaaaattc aatcgcctgc gaagtatatc gctggagcga tggatcgtat
     1861 ctcgaagatc aggatttctg gcggttatcg ggctttgaaa gaagtattta tttgtatagc
     1921 actgatcaga gccgcatttt tgactttttc gttaaaacag atttggataa ggcatataaa
     1981 aatgcaatgc tatcattgga tattaagttg aaaaactata ccaatacgaa gaaaaagcaa
     2041 attgttgaaa ttaagttgtt ggattccaat atgaaagcgg ttttcacgca acaaaaggtg
     2101 gttcagatag agggtgaaaa ttcggttgaa tccagttttg tgcaaaaagt aagtgcacca
     2161 aaactctgga cggctgaaac accaaatttg tacacattgg tgttgacttt aaaaaatgag
     2221 aatggaaaag cgatagaatt tacatcgacc aaggttggtt tccggaaaat tgaaatcaaa
     2281 gacggtcagt tgctggtaaa tggaaaacgt gttttgttta aaggagtgaa tattcatgag
     2341 cacaaccatt taaccggaca tactataaac agagaaatta tgatgcagga tatccggctg
     2401 atgaagcagt ttaatatcaa tgctgttcgt acgagtcatt atcctcagcc tgtgctttgg
     2461 tacaaactgt gcgacgaata tggaatttat ctgattgatg aagccaatat tgagtctcac
     2521 ggaatgggtt atggaaaaga atcaattaca cattttcccg aatggtggga tgcgcatttg
     2581 gatcgtacaa ttcgcctggt tgaacgcgac aaaaatcacc catcggttat tgtgtggtcg
     2641 ttgggcaacg aatcggccaa tggaaaagcc tttgaaataa cttacgattg ggttaaaaac
     2701 agggataata cccgcccggt acaatacgag caagctcacg aagaacgtaa tactgatatt
     2761 atttgtccca tgtatccgga ctttgaacac atgaggaaat ttgcatcccg taaactcgat
     2821 cgtccttaca ttatgtgcga atatgctcac gccatgggca acagtatagg gaattttcag
     2881 gaatactggg atatcattcg aagcagtaaa cattttcagg gtggctatat ctgggactgg
     2941 gttgaccagg gattgttgca aaaagatgag aatggacggg aatattgggc ttatggaggc
     3001 gattttggtg ttggattcga atatcacaac gatgctaact tctgtgccaa cggcttggtt
     3061 gcccccgatc gtactcctca cccacatctg tacgaagtga aaaaagtata tcaaaatgtg
     3121 cttatcaatg ctaaggattt gtcgaaagga attgtaactg taacgaacga ttacaggttt
     3181 actaacctga aagatatttt gtatttcgaa tggcatttac tcaaaaacgg agtaaaagta
     3241 tcgcagggga aatttgatgt tgatcttgct cccgaagcat cgaaagatgt cagattggct
     3301 ttacctgaat taaaaaagca gcagggtgat gaatatttcc ttcagattta tggctacacc
     3361 ttaaataaaa cagaactgat tcctgcaaat tttgaattgg ttcgggagga atttgcttta
     3421 tcggggaatg attattttac tcttacaaaa ctttcgactt caaaacctca atttgttgca
     3481 aaagacaacg tgattcatgt tacggccgga gatcttttgg ctgaatttca taaatcggtt
     3541 gcaggtttgg tcgaattgtc atataaagga attcagttgt taaaaggaaa atcaccggaa
     3601 ctaaatttct ggcgtgctcc tacagataac gattggggcg aaggtgctca caaaaaaatg
     3661 aatatttggc gcaatgcagg caatagcgtg gtactcaaag atgtagtttt agtcgaagga
     3721 gaaaataaat tacagattaa ctatcatctg ttttcaaccg atattcaaag cgatattttc
     3781 attaattata ccgtttccgg tgatggaagt attgtttgtg atatagaata taaaactcaa
     3841 aataactttt tacctgaaat gccccgtttt ggaatcatgt tcaccttgcc cgaagaattt
     3901 gaaaatctga gttggtatgg acgcggacca tgggaaaatt atatcgaccg caatgtttcg
     3961 agttttatgg gaatatatga aagtaaagta aaagatcaat actatcccta tgttcgtcca
     4021 caggaaaatg gcaataaaac tgatatccgt tggataacac ttaccaataa cgaaggggtt
     4081 ggattgaagg tagaaggtct tcagcccatt tcattcagtg ctttaaatta tcgtccggaa
     4141 gacttcgatc ccgggttttc gaaaaaacaa cagcatatca gcgatatgta tccgcgccgc
     4201 gaagtggttt tgtcagtcga tttgtttcaa cggggtttgg gtggattaaa ttcatgggga
     4261 gcaggcccat tgaaacagta tcggtttact gaaaaaattt attcatactc ctttaaaatt
     4321 tctggggtag aataaatgcc ataagaattt gcattattta aaatttataa gatgttcaat
     4381 agtgtgttat atttttaatt aaataattga aaatcaataa attatttcaa tttttatttg
     4441 gttaattcac tgaaaataag tatctttata gattaatcaa ctaacttatt atataatttc
     4501 taataattaa ttgcacataa tattttcaaa gtctcattcg gct
//
LOCUS       DAOSCH010000135         4531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_12064_length_4531_cov_6.348972, whole genome shotgun sequence.
ACCESSION   DAOSCH010000135 DAOSCH010000000
VERSION     DAOSCH010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12064_length_4531_cov_6.348972"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            98..973
                     /locus_tag="PLZ54_08280"
     CDS             98..973
                     /locus_tag="PLZ54_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOJ66954.1"
                     /translation="MYSVFIRRFIYALILGSSVIFLACCKNRTSNTKFTDKPLVEVEG
                     NYLFRYQIEQIIPPNVNKKDSEELANNFIKNWATNVLMYERAKQNIEDISEIEKLVEE
                     YRKTLIIHAYQQKLIQQRLSTDISEEEIKSFYEKFGNQIVLNESYVKGLLLVVPISAP
                     QLNEVRKWVQQPTVKSLENIEKYRLKNAISYEYFMNKWVPFLEILKKIPLELTKTPAE
                     FLSANVFVETSDSTQIYLLKIQEYLNQGDKKPYELAKEEIKDILRHQQEVALIKQFEN
                     DLYNDALTKKKIIFY"
     gene            1042..2403
                     /locus_tag="PLZ54_08285"
     CDS             1042..2403
                     /locus_tag="PLZ54_08285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOJ66955.1"
                     /translation="MQTKRFVFFILLVSFFPFIHLTAQNNVIDEVIWIVGDDAILKSE
                     VEEQRLRAQYEGVPIQGDPYCVIPEQIAIQKLFLHQAQLDSIVANESTVNSQVEARLN
                     YFVSQIGSKEKMEEYFGKKTTELREDLRQIIREQMIIQQMQQKLVGDINPTPAEVRRF
                     FKQLPEDSIPVVPAQVELQIISYQPPVSQQEINRVKQRLLDFTERINSGNADFSVLAR
                     LYSEDVESAKRGGELGFLGRGQLVPEFAAVAFNLQDPKKVSRIVETEFGFHIIQLIEK
                     RGDRINCRHILLRPRISEEEKEKALHTMDSIANLIRSGKITFEQAVMYYSQDKNTALN
                     AGLMLNEKTGTSKFEYQDLPPEISKIAYNMNVGEISNPFIMIDPKTHKEVIAIVKVKS
                     KIPNHKANLVDDYQLIKTFYENKKKEEFLRDWIIKKQKETYISIDPAWRNCEFQYPGW
                     IKN"
     gene            2473..4299
                     /locus_tag="PLZ54_08290"
     CDS             2473..4299
                     /locus_tag="PLZ54_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOJ66956.1"
                     /translation="MNLLHLDSVSSFFQIKLLFPLFLMVGIITSPALAQNRPKQLREE
                     ILNSQKEAETKSAKSTKKKELSKPQTVLPPLTPVMPIVNPLENEKATLIYLENSETVT
                     FDKFLNPDIQVLKGNVQFRHENALMYCDSAYFYKATNSLDAFGNVRMVQGDTLFVYGN
                     VLYYDGFTKLARLRKNVRLENRKTVLTTDSMNYDRITNLAYYYTGGKIKDEVNTLTSV
                     WGQYSPATDQALFKDKVKLVNKNYTMDADTLKYNTKTNIANIVGPTHILYKNETDIYS
                     TRGWYNTSTERSMLLDRSLVIHKDGKTLTGDSIFYDKKQKYAEAFQRVILTDTVQKTT
                     LTGNFVSYNENTEFGMANDSALLVEWSTQDSLFLHADTLFTFKDSVYNAARAYYNVRF
                     FRNDLQGVCDSLYYSARDSVVRMYKEPVLWAEDFQITGDSISGYIKNKKIDHIRVLPS
                     AIAIQQFDSIHFNQISGKEIVAYLDSNQLKRVHVNGNAETIYYPLDEKDSTLIGLNKT
                     QSSYVVVYFKDKKVDRIVLTNASNGIMYPLNQLSGGDIYLKNYIWLDYLRPKSKKEVF
                     LKISRQPAARIGTSSLMNTTVNQSSSENTERNSRKSPGMRNP"
BASE COUNT         1569 a          737 c          825 g         1400 t
ORIGIN      
        1 ccgttaagaa aaactaatat tttttgacag gtgcatagaa atcactaatt ttatatttcc
       61 aatttcgaga aatcgaaacg aattatataa ttgtttgatg tacagtgttt ttattcggag
      121 gtttatttat gctttgatat tgggaagcag tgtgattttt ttggcttgtt gcaaaaatcg
      181 tacttcgaac acgaaattta ccgataaacc acttgtagaa gtggaaggaa actatctttt
      241 tcgttatcaa attgaacaaa tcattcctcc caatgtaaat aaaaaagata gtgaagagct
      301 ggcaaataat tttatcaaaa attgggctac aaatgtcttg atgtacgaac gtgccaagca
      361 aaatattgaa gatatttccg aaattgaaaa attggtagag gaatatcgta aaacattaat
      421 tattcatgca taccagcaaa agttaattca acaaaggctc tcaacagaca tttctgagga
      481 agaaataaaa tcattttacg aaaaatttgg taaccagata gtgctcaatg aatcttatgt
      541 aaaaggatta ttacttgtgg tgcccatatc tgctcctcaa ttaaacgaag tgagaaaatg
      601 ggtgcaacag cctacagtaa aatcattgga gaatatagaa aaatatcgat taaagaatgc
      661 tatcagttac gaatatttta tgaataagtg ggtccctttt ttggaaattt tgaagaaaat
      721 ccctttagaa ttgacaaaaa cgcctgcaga atttttgtca gcaaatgttt ttgttgaaac
      781 ttcagattct actcaaattt atttattaaa aattcaggaa tatcttaatc aaggtgataa
      841 aaaaccgtat gaattggcaa aagaagaaat taaagatatt ttgagacatc agcaagaagt
      901 agctctcatc aaacagtttg aaaacgattt gtataacgat gctttaacca agaagaaaat
      961 aattttttat taaacagtaa ttagtattct caattattct gatcttgttt tctttttatt
     1021 tttttaattt taattagttt catgcaaaca aaaagatttg ttttttttat tctacttgtt
     1081 tcctttttcc catttatcca tttgacagct cagaacaatg tgattgatga agtcatttgg
     1141 attgtaggag atgatgctat tttgaaatcg gaagtagagg aacagcgttt gcgtgctcaa
     1201 tatgaaggcg tccccattca aggagatcca tattgtgtga tacccgagca aatagccatt
     1261 caaaagttat ttttgcatca agcccaactt gatagtattg tagccaatga aagcacagta
     1321 aattcccaag tggaagctcg tttaaattat tttgtttcac aaattggctc taaggaaaaa
     1381 atggaagaat attttggcaa aaaaaccacc gaattgcgag aagatcttcg tcaaataatt
     1441 cgggaacaga tgatcataca gcaaatgcag caaaaactgg ttggagacat aaatcctact
     1501 cctgctgaag tgagaagatt tttcaaacaa cttccagagg atagcattcc tgttgtaccg
     1561 gctcaagttg aattacaaat tatcagttat cagccacctg tatctcaaca ggaaataaac
     1621 agagtaaaac agcgtttact ggattttacc gagcgtatta atagtggaaa tgctgatttt
     1681 tctgtattgg ccaggcttta ttccgaagat gtggaaagcg ccaagcgtgg aggagaattg
     1741 ggttttttgg gtagaggaca actggtaccc gaatttgcag ctgtagcttt taatttacag
     1801 gatcccaaaa aagtatcccg cattgtagaa acagaatttg gtttccatat cattcaactg
     1861 atagaaaaac gaggtgatcg tataaattgc cgacatatat tgctcaggcc acgcatttca
     1921 gaagaagaaa aagaaaaagc cttgcacact atggattcca ttgccaattt aataagaagc
     1981 ggaaaaatta cttttgaaca ggcagtaatg tattattcgc aagataaaaa cacggctttg
     2041 aatgcaggcc tgatgctgaa tgaaaaaacc ggaacttcaa agtttgaata ccaagatctc
     2101 ccgcctgaaa tttctaaaat agcttataat atgaatgttg gagaaatttc aaatcctttt
     2161 ataatgattg atcccaaaac ccataaggaa gtgatagcta tagtaaaagt taaatcaaaa
     2221 attcctaatc ataaggcaaa tttagtggat gattatcaac tgattaaaac tttttatgaa
     2281 aacaaaaaga aagaagaatt tcttcgggat tggattatta agaaacagaa agagacttat
     2341 atcagcattg accctgcatg gagaaattgt gagttccaat accctggttg gataaaaaat
     2401 taaatcatgt gttgatgctt atattgcttt caagttattc gcatttgttt tttaatagta
     2461 taatcatttt tcatgaattt actccatctt gatagcgtct cttctttttt tcaaataaaa
     2521 ttgttatttc cacttttttt gatggtggga ataataactt cccccgcttt agctcaaaat
     2581 aggccaaagc agttgcgcga agaaattctg aatagtcaaa aagaagcaga gacgaaatct
     2641 gcaaaaagta cgaaaaagaa ggaactttca aaacctcaaa ccgttcttcc gcctctaact
     2701 cctgtaatgc ctattgtaaa tcctctggaa aacgaaaaag ctaccttaat ttaccttgaa
     2761 aattctgaaa ccgtaacttt cgataagttt ttaaaccccg atattcaggt tttgaagggg
     2821 aatgttcaat tcaggcacga aaatgcttta atgtattgcg atagtgcgta tttttacaag
     2881 gctaccaatt cattggatgc tttcggtaat gtaagaatgg tacagggaga caccttgttt
     2941 gtgtatggaa atgttcttta ttatgatggt ttcaccaagt tggcgcgttt acgaaaaaat
     3001 gtgaggctcg aaaatcggaa aacggttctt actaccgaca gtatgaatta cgaccgaatt
     3061 acaaatctgg cttattatta taccggaggg aaaataaaag atgaagtaaa taccttgact
     3121 tctgtttggg ggcaatactc gcctgcaact gaccaggctc tgtttaaaga taaagtaaag
     3181 ctggtaaaca aaaattacac aatggatgcc gatacgttga aatacaacac aaaaacaaac
     3241 attgccaata ttgtcgggcc aacgcatatt ctttataaaa acgaaaccga tatttattcc
     3301 actcgtggat ggtataatac cagtaccgaa cgttccatgt tgttggacag atcgttagta
     3361 attcataaag atggaaaaac cctcacgggc gattcaatat tttacgataa aaaacaaaaa
     3421 tatgccgagg cttttcaaag agtaatattg actgatacag tgcagaaaac caccttaacg
     3481 ggaaatttcg tttcttataa tgagaatacc gaatttggta tggctaacga ttcggcattg
     3541 cttgtcgagt ggtcaactca ggatagtttg tttttgcatg ccgacacgct gttcactttc
     3601 aaggattccg tatacaatgc agctcgcgca tattataacg tacgtttttt cagaaacgat
     3661 ttgcagggag tttgcgattc cctttattac tcggctcgag attccgtagt ccgtatgtac
     3721 aaggaacccg ttttgtgggc ggaagatttt caaataacag gggattcgat aagcggttat
     3781 attaaaaata aaaaaattga tcatattcgt gttttgcctt ctgcaattgc catacagcag
     3841 ttcgactcca ttcattttaa ccagatttcg ggaaaagaaa ttgttgctta tttggacagc
     3901 aatcagttga aacgtgtaca tgtaaatgga aatgccgaaa caatatatta tccgcttgac
     3961 gagaaagatt caactctcat tggactcaat aaaacacaaa gcagttatgt agtggtatat
     4021 tttaaggata aaaaagtgga tagaattgtc ttaaccaatg catcaaatgg tatcatgtat
     4081 ccactcaatc aactttcggg aggcgatatc tatctgaaaa attatatttg gcttgattat
     4141 ttgagaccta aaagcaaaaa agaagttttt ttgaaaattt ccagacaacc tgctgcccga
     4201 ataggtacca gcagcttgat gaatactaca gttaatcagt catcatccga aaatacggaa
     4261 agaaattcga gaaaatctcc gggtatgcga aatccataat tttgatttaa atgccatgtg
     4321 catactcttt ctgaatcgtt ttatttctat tttagaaata gttttttgtt tttttgtcca
     4381 tcaaaataat aagaaactat cttcaatgaa cacatccttc tcctgttgca tccgggttca
     4441 acttcaaaaa cagaaaaaat ctttggaaaa aatttaaatg attatccgca atgattccta
     4501 ttgtaaattc ttgacttgaa atcggagttg t
//
LOCUS       DAOSCH010000136         4505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_12142_length_4505_cov_5.282247, whole genome shotgun sequence.
ACCESSION   DAOSCH010000136 DAOSCH010000000
VERSION     DAOSCH010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12142_length_4505_cov_5.282247"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLZ54_08295"
     CDS             <1..261
                     /locus_tag="PLZ54_08295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ66957.1"
                     /translation="ISPLEAMQVGTPTIISKQSGCSEILTHSIKIDYWDIDGMADAVN
                     AIIRNPAMYKSLHELGREEVNNIRWEDAGKKVIKIYNSLLGK"
     gene            443..1729
                     /locus_tag="PLZ54_08300"
     CDS             443..1729
                     /locus_tag="PLZ54_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOJ66958.1"
                     /translation="MKNLCFYFEIHQPMRLKRYRFFDIEQDHYYYDDFQNEERIRMLA
                     EQSFLPANRTLLEIIQNSNGKFRCAFSFSGIVLEQLEQYAPEVIDSFEELSKTGCVEF
                     MAETYAHSLSSVYDIHEFEQQVALQTEKIESLFGKKPTVFRNSELIYSDEIGEKIYNM
                     GYRTILIDEVSHVLGWKSPNFVYRHPTLPKLKLLVRNFKLSDDIAFHFSDRSWSDYPL
                     TADKFISWIKNMPDDQPVVNIWLGYEALGLFQRPESGIFEFLKAIPFFALQNNIGFLM
                     PSEVAKKIEAVDILSSPEPISWSGIEKDISLWNGNDLQQEALEKLYAVGERVRLCTDR
                     PLRHDWLMLQSSDHFRYMSHKDAFGTYYESPYDAFMNYMNILADFLLRVDEQYPTTID
                     NEELNALLNTIYELEKEVERLENELKKLRSRKKTEN"
     gene            1741..2328
                     /locus_tag="PLZ54_08305"
     CDS             1741..2328
                     /locus_tag="PLZ54_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66959.1"
                     /translation="MVHEFGKSLHYEKSQFRRADAFYREVLLVDEIIRFATDSKEDMK
                     MQLQDIDVQLIKTNRVCHVSEKFREKDFVDLYVEVFSKYPHTRGWLETGLADMLVYFV
                     PEVVYWCNAKNLRKFCVDMLFPMIPAEWYKEIYCSATSIISKNMNFFDQQTIVHLIKA
                     NNQTSDGNRWCTIGISVPFEFLKNNGLNIRKYLFP"
     gene            2481..3200
                     /gene="hisA"
                     /locus_tag="PLZ54_08310"
     CDS             2481..3200
                     /gene="hisA"
                     /locus_tag="PLZ54_08310"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HOJ66960.1"
                     /translation="MEIIPAIDIIDGKCVRLSQGNYDKVTLYSRYPDEIAKMFADEGF
                     RRLHLVDLDGAKAQHIVNYKVLEKITSTTDLVVDFGGGLKSDDDLRIAFECGASMVTG
                     GSIAVKNPEIFVGWLEKYGGEKIILGADVKNEKIAVSGWTESTELELIPFVHNYFSKG
                     VKYVICTDISCDGMLQGPAIELYKKIISAEPQVHLIASGGVGSLHDIEMLNEAGIPAV
                     IVGKAIYEGNISLQELSRFLF"
     gene            3245..3703
                     /locus_tag="PLZ54_08315"
     CDS             3245..3703
                     /locus_tag="PLZ54_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOJ66961.1"
                     /translation="MLKEILYVSGKPGLYKMVSQGKNLMVIESLSDGKRIPVFSSDKL
                     VYLKDIAIFTENGEIPLSEVFEKIKVKENGAKCAVDAKSGNEKLVQYLQEILPDYDKN
                     RVYPSDIRKLINWYNILVDAKLTDFAASETETGDKVETVSDKTEEISSEK"
     gene            3718..>4505
                     /locus_tag="PLZ54_08320"
     CDS             3718..>4505
                     /locus_tag="PLZ54_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOJ66962.1"
                     /translation="MKVQTVCNILEEFAPLALQEAYDNAGLIVGDPFMEVNGILISLD
                     VTMEVLEEAKRKKCNFILAHHPLIFNSLKRLVGQNETERCVIFALKHDLAIYAGHTNV
                     DNLWNGVNQKIAEKLQLVHTKVLKPLSHQLLKLVTFVPHAHADKVRKAMFEAGAGSIG
                     NYDSCSYNVEGTGTFKAQENAHPFVGEHNEFHAEPETRIEVILPSYLKNSVVNALLQA
                     HPYEEPAYDLIPLENEWNRAGAGLLGNLEIPTDELAFLHRLKTV"
BASE COUNT         1455 a          727 c          920 g         1403 t
ORIGIN      
        1 atttctcctt tagaagccat gcaagtgggt acaccgacca ttatttcaaa acagtcggga
       61 tgttctgaaa ttttaacaca ttcaattaaa attgactatt gggatatcga tggtatggcc
      121 gatgcagtaa atgcaattat caggaatccg gccatgtata aaagccttca cgaattgggg
      181 agagaagaag taaacaacat tcgttgggaa gatgcaggta aaaaagtaat taaaatatac
      241 aatagtctgt tggggaaata aattccttta aagaaatttt cttttttaga aatcaacctc
      301 aataagaaaa ttgtcttatt gataatcaaa tttataaatt aaaccaatct ttatcgtttg
      361 aatagattta cacaattttt ggagctgaaa tatctgaaaa aagtaaatcc aatttcattc
      421 ctaaagaaaa aaggataaat gtatgaaaaa tctgtgtttt tattttgaaa ttcatcagcc
      481 aatgcgatta aaaagatatc gtttcttcga cattgaacag gatcattatt attatgatga
      541 ttttcagaat gaagaacgaa ttcgtatgtt ggctgaacag tcgtttttgc cggccaatcg
      601 cacgcttttg gaaattatcc aaaattccaa tggaaaattt cgttgcgctt tttcgttttc
      661 gggaattgtt ttggaacagc tggaacagta tgctcccgaa gtaatcgaca gtttcgaaga
      721 actttccaaa accggctgtg tggagtttat ggccgaaact tatgctcatt ctctttcatc
      781 tgtttacgat atacatgaat ttgaacagca agtagccctg caaaccgaaa aaatagagtc
      841 actttttgga aaaaaaccga ctgtattccg aaattcggag ttgatttact cggacgaaat
      901 tggagaaaaa atctacaata tgggatatag aaccatattg attgatgaag tcagccatgt
      961 tttgggttgg aaaagtccaa atttcgttta tcggcacccg acgttaccaa aattaaaatt
     1021 gctggttcgt aattttaagt tgagcgacga tattgctttt catttttctg atcgctcgtg
     1081 gagcgattac cctcttacag ccgacaaatt tatttcatgg ataaaaaata tgcccgatga
     1141 tcagcctgtt gtaaatatct ggcttggcta cgaagcattg ggtttgtttc agcggcctga
     1201 aagtggaatt tttgaatttc tcaaagctat tccttttttt gccttgcaaa acaatatcgg
     1261 ttttttaatg ccttcggaag tggcaaaaaa gattgaagca gttgatattc tttcttctcc
     1321 cgaacccatt agttggtccg gaatagaaaa agacataagt ttgtggaatg gaaacgacct
     1381 tcagcaggaa gcactcgaaa aactttatgc ggtaggcgag agggttcgcc tttgtaccga
     1441 cagaccgctt cgacacgatt ggcttatgtt gcagtcatcc gatcattttc gatacatgag
     1501 tcataaggac gcttttggaa cttattacga atcgccctat gatgcgttta tgaattatat
     1561 gaatattttg gctgattttc tgctgagagt tgatgaacaa tatcccacta ctattgataa
     1621 cgaagaactt aatgctttgc tcaataccat ttacgaactc gaaaaagaag ttgaacgttt
     1681 ggaaaacgaa ttgaaaaaat tgcgttcgag aaaaaagact gaaaattaaa attgattttc
     1741 atggtgcatg agtttggaaa aagtcttcat tacgaaaaaa gtcaatttcg gcgcgccgat
     1801 gcgttttatc gtgaagtttt actcgtagat gaaatcatac gatttgcaac agactcgaaa
     1861 gaagatatga aaatgcagct tcaggatatc gacgtgcaac tgataaaaac caatcgagtc
     1921 tgccatgttt ccgaaaaatt cagagagaag gatttcgtcg atttgtatgt tgaagttttt
     1981 agcaaatatc ctcatacgcg cgggtggcta gaaaccggtt tggccgatat gttggtgtat
     2041 tttgttcccg aagtcgtcta ttggtgcaat gctaaaaatc tcagaaaatt ttgtgtggat
     2101 atgctgtttc caatgattcc tgctgaatgg tacaaggaaa tttattgttc cgctacttcg
     2161 attatttcca aaaacatgaa ttttttcgat cagcaaacca ttgttcatct gatcaaagca
     2221 aataatcaaa catctgatgg gaaccgttgg tgtaccattg gaataagtgt tccttttgag
     2281 tttctgaaaa ataacggatt aaatatcaga aaatatttgt ttccgtaatt ttttgagtga
     2341 tgaatttttt agctgtttat tttgtagaaa tatcgattat tcacagctat aatcaaaata
     2401 gttgttgact tgtttcacat tttctttttt tatacttttg ccaacttgtt tgaagaaaat
     2461 agatacaaaa aacatcataa atggaaatta ttccggccat tgacattatt gacggtaaat
     2521 gtgttcgctt gtctcaaggc aactacgata aagtaacgct ctacagccgc tatcccgatg
     2581 aaattgctaa aatgtttgct gatgaaggtt tcagacgatt gcatttggta gatttggatg
     2641 gagcaaaggc tcaacacatt gtcaattaca aggtattgga gaaaattact tctactaccg
     2701 acctcgtagt tgatttcggc ggaggattga agtcggacga tgatttgcgt attgcttttg
     2761 aatgtggagc ttcaatggta acgggaggaa gcattgccgt aaaaaatcct gaaatcttcg
     2821 ttggctggct cgaaaaatac ggcggagaaa aaattatttt gggagctgat gttaaaaacg
     2881 aaaaaattgc cgtatcgggc tggactgaaa gtactgaatt ggaattgatt ccttttgttc
     2941 ataattattt ttcaaaagga gttaaatacg ttatttgtac tgacattagt tgtgatggaa
     3001 tgttgcaagg accggcaatt gaactctaca aaaaaataat ttctgccgaa cctcaggttc
     3061 atttgatagc aagcggtgga gtgggttcac ttcacgatat agaaatgtta aatgaagcag
     3121 gtattccagc agtcatagtc gggaaagcaa tttacgaggg taatatttca ttacaagagt
     3181 taagtcgatt tttattttaa attttaaaca ttaaaatagt caaattattt gtaattaata
     3241 agttatgttg aaagaaattt tatatgtttc cggcaaaccg ggtctttaca aaatggtatc
     3301 acagggaaaa aatctcatgg ttattgaatc cctgagcgat ggaaaacgta ttccggtttt
     3361 ttcaagcgac aaattagtat atttgaaaga tatagctatt tttactgaaa atggcgaaat
     3421 tccattgtct gaagtttttg agaaaatcaa ggtgaaagaa aatggagcaa aatgtgctgt
     3481 cgatgctaaa tcgggaaatg aaaagctggt tcaatacctt caagaaattc ttcctgatta
     3541 tgataaaaac agggtttatc caagcgacat ccggaaatta atcaattggt acaacatttt
     3601 ggtagatgcc aaattgaccg attttgcagc atctgaaact gaaaccggcg acaaggttga
     3661 aaccgtatca gataaaacag aagaaatttc ttcagaaaaa taaattcgtt ttcagccatg
     3721 aaagttcaaa cagtttgcaa tattctggaa gaatttgcac cattggcgct acaggaagct
     3781 tatgataacg cagggctgat agtgggcgat cctttcatgg aagttaacgg tattttgatt
     3841 agtctcgacg ttacgatgga agtattggaa gaagcaaagc gaaaaaaatg caattttatt
     3901 ttggctcatc atcctttgat tttcaattcg ttaaaacgtc ttgttggtca gaatgaaaca
     3961 gaacgttgcg ttatttttgc gttgaaacac gatttggcaa tttacgctgg ccataccaat
     4021 gtggacaatc tttggaatgg agtcaaccaa aaaattgccg aaaaattgca attggttcat
     4081 acaaaagttt taaaaccttt gtctcatcaa ttgctcaagc tggttacttt tgttcctcat
     4141 gctcatgccg acaaggttag gaaagcaatg tttgaagcag gtgccggcag catcggcaat
     4201 tacgattcgt gcagctataa tgtagaagga acaggaactt ttaaagcaca agaaaatgcc
     4261 catccatttg ttggcgagca caatgaattt catgcagaac ccgaaaccag aatagaagta
     4321 attttaccga gttatttgaa aaattcggtt gttaatgcgt tgttacaagc ccatccttac
     4381 gaagagcctg cttatgattt gattcctctg gaaaatgaat ggaacagggc aggagcgggg
     4441 ctattgggta accttgaaat accaacagat gaacttgctt ttctacatcg tttgaaaacc
     4501 gtttt
//
LOCUS       DAOSCH010000137         4498 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_12165_length_4498_cov_5.998875, whole genome shotgun sequence.
ACCESSION   DAOSCH010000137 DAOSCH010000000
VERSION     DAOSCH010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12165_length_4498_cov_5.998875"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2798
                     /locus_tag="PLZ54_08325"
     CDS             <1..2798
                     /locus_tag="PLZ54_08325"
                     /inference="COORDINATES: protein motif:HMM:NF026255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOJ66963.1"
                     /translation="KKTSVLRSVAIILATFFSSLLFAQSAIEGKVLDKKTKEPLVGAS
                     ILIEGTSRGTTTDIDGNFKISELDPGKYKLIVSYVSYKKLEFDEIKIQKNIPTILNIE
                     MEEAELMLQSVQVVAQHRTDTELSLLKSVRTAVQVVNGISSQQIGKTLDRDAAEVVKR
                     IPGVTVQNDRFVVVRGLNQRYNNVWLNNASTPSSETDVKAFSFDAVPSNMIDNIMIYK
                     TGSPELSAEATGGFVKIFTKNIPEENFQQIEYSFGYNDQTSFKNSYHLPTQTLDVFGL
                     GSLQRDLPADFPANLNSLSIQQRDAAALKLNNHWVAQPYTALPNQKLTFSIGRKWNLK
                     KGNKIGNLTSLTYSNSSSSRFNMENNMYERFDPIAETPVYLYNYAADIYSTDFKIGLM
                     HNWAYIKTNGTKIEFKNLLNQIGVDKTSNTFGWNNYRQGNFKYFSNQYSARTTYSGQL
                     SGNHKLKNNEDNKLDWNVGYAYANRIEPDRQNWSMKETQGGIYEYVLPDVPSINELGR
                     LYMNNHENVMVSGINHERKFQFLNTLPVLKSGLYGEFKSRNFSERSITYRKAYGPLGN
                     DEINALDFESLFTETYLGQGKVISVDEQTNVANTYKAQNWLGAGYLSMTLPAGKFNLS
                     GGIRAEYNHLLLDGYYNPTSPVHVDNPTFNIFPSINTSYNLSDKSLIRLAYAHTINRP
                     EFREVSPLSYYDFTEKNSVRGNPNLQDASIQNIDLRFENYPSPTETFSLAAFYKHFTH
                     PIEMVNNGVGNDFSFGNAQAAVNYGLELEAKKSLEKLIGLKNFTLQVNASYIFSKVEF
                     ADKANEQNRPLQGQSPYVVNAALFYQNDKAGLSTSVMYNVMGKRILAAAQLNQGEVVI
                     PDIYEMPRHVVDFTLNKKLGNQMELKFGIKDLLAQNYKTQQTYEYEKDGTAKKATLTN
                     QSYNLGRTFSLALNCKF"
     gene            3032..4420
                     /locus_tag="PLZ54_08330"
     CDS             3032..4420
                     /locus_tag="PLZ54_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66964.1"
                     /translation="MKRKFLNYFVISSVMVSGLIFFTACDDKSDPDPEPTPTSNEVIL
                     NSDITGTRNLVNDSVYILQGQVNVSGTINIEKGTIIKGDKLTKGCLVVLRGGVINAIG
                     TPTEPIVFTSRMEPGLRNAGDWGGIIIVGNAPVNQSNPSVEGLTREVTYGGGTNPADN
                     SGSLRYVRIEFAGIALQPDKEINGLTLCAVGSGTTIDHIQVSYSGDDSFEWFGGTVNA
                     KYLIAYCGLDDEFDTDFGYSGRVQFALGVRNPRVADVSTSNGFESDNDGSGTGATPQT
                     SAVFSNVTLIGPYKTTADKNVNSMFGAGMHIRRNSSISVFNSVFAGWNTGLLLDGTST
                     YANATASNLVIQNCVLSGTKGTAVNGAGGVTADQALTFFNSGNNQIIADNATTGIASS
                     FYDATKTGAEAAPSSFLPAAGSPLLSGASFTHAKLADNFFDKTPTFKGAFGTTDWTKE
                     QWTNFDPQNTVY"
BASE COUNT         1448 a          886 c          893 g         1271 t
ORIGIN      
        1 tgaaaaaaac ttcagtatta cgctctgttg caataatttt agcaacattt ttctcctcgc
       61 ttctgtttgc tcaaagtgcg atagaaggaa aagttttaga caaaaaaacc aaagaaccac
      121 tggtaggtgc cagtattcta attgaaggaa cctcacgggg aacaactacc gacattgacg
      181 gaaatttcaa aataagcgaa cttgatcccg gaaaatataa actgatagtg tcgtacgttt
      241 cttataaaaa acttgaattt gacgaaatta aaattcaaaa aaatatacct acgattttaa
      301 acatagaaat ggaagaagcc gaattaatgc ttcaaagtgt acaggtagtg gcacaacatc
      361 gcacggatac agaattatcg ctgctgaaaa gtgtacgcac ggccgttcag gtggttaacg
      421 gcatttcttc ccagcaaatc ggaaaaacac tggatcggga tgcggcagaa gtagtgaagc
      481 gaatccctgg cgttacggta caaaacgacc gatttgtagt agtacgcggt ctcaatcagc
      541 ggtacaataa tgtttggctc aacaatgctt ccacaccaag tagcgaaaca gatgtgaaag
      601 cattctcctt cgacgctgtt ccgagcaaca tgattgacaa tattatgatt tataaaacag
      661 gttctcctga actttctgcc gaagcaacgg gaggatttgt aaaaattttt accaaaaata
      721 tcccggaaga aaattttcaa caaatcgaat acagttttgg atacaatgat cagacttcat
      781 ttaaaaacag ttatcactta ccaacacaaa cattggacgt ttttggtttg ggtagcctgc
      841 aaagagatct tcctgccgac ttccccgcaa atttgaactc cctcagcatt caacagcggg
      901 atgctgcagc tttaaaactg aacaaccact gggttgctca accttacacg gcattgccaa
      961 atcaaaaatt gactttttct attggaagaa aatggaattt gaaaaaaggg aacaaaatag
     1021 gtaaccttac ttcactcacc tacagcaact catcttcttc acgtttcaat atggagaata
     1081 acatgtacga acgctttgac cccatagcag aaacacctgt ttatctttac aactatgcag
     1141 ccgacatcta cagtaccgat tttaaaatcg gactgatgca caattgggct tacattaaaa
     1201 caaatggaac aaaaatagaa tttaaaaacc tgttgaacca aattggagta gataaaactt
     1261 ccaatacttt tggctggaac aattaccgtc aaggaaattt caaatacttc agcaatcagt
     1321 attctgcacg taccacctat tcaggacagt tgagcggtaa ccacaagttg aaaaacaatg
     1381 aagacaacaa actggattgg aatgtgggtt atgcttacgc caaccgaatc gaaccggatc
     1441 gtcaaaattg gagcatgaaa gaaactcaag gaggaattta cgaatatgtg ttgcctgatg
     1501 ttcccagcat taacgaattg ggaaggcttt acatgaacaa tcacgaaaat gttatggtgt
     1561 ctgggataaa tcacgaaaga aaattccaat ttctcaatac gttgcctgtt ttgaaatcag
     1621 gactctatgg ggaattcaaa tcgcgcaatt tttcggaacg aagcattacc tatcgcaagg
     1681 catacggacc attgggtaat gatgagatta atgcactcga ctttgaaagt cttttcactg
     1741 aaacttacct cggacaagga aaagttatta gcgtagatga acaaaccaac gttgctaaca
     1801 cctacaaggc acagaattgg cttggtgctg gatatttgtc catgacattg ccggcaggaa
     1861 aattcaattt gagtggagga atcagagcag aatacaatca tttgctgctg gatggctact
     1921 acaatccaac ttcaccagtg cacgttgata atcccacttt caatattttt ccttccataa
     1981 atacttctta caatttatcc gacaaatctc ttatcaggtt agcttatgct cataccatca
     2041 atcgccccga atttcgtgaa gtttcgcctt tgagttacta tgattttacc gaaaaaaatt
     2101 cagtaagagg taatcccaat ttgcaggatg cttccattca aaatatcgat ttgcgatttg
     2161 aaaactatcc atctccgaca gaaacttttt cgctggcagc tttctacaag catttcacgc
     2221 acccgatcga aatggtaaac aatggcgtag gaaacgattt ctctttcgga aacgcccaag
     2281 cggctgttaa ttacggactg gaactggaag caaaaaaatc gctggaaaaa cttatcgggt
     2341 taaagaattt tactcttcag gtgaatgcat cctacatttt cagtaaggtg gaatttgccg
     2401 ataaggcaaa cgaacaaaat cgaccattgc agggacaatc gccgtatgta gttaacgcag
     2461 cgttatttta ccagaatgat aaagccggtt tgtcaacttc tgtgatgtac aatgtgatgg
     2521 gaaaaagaat tttggctgca gcacagttga atcagggaga agtagtaatc cccgatattt
     2581 atgaaatgcc tcgtcatgtg gtcgatttca cattaaacaa aaagctgggc aatcagatgg
     2641 agttaaaatt tggtatcaag gacttgcttg ctcaaaatta caaaactcag caaacctatg
     2701 agtatgaaaa agatggtaca gcaaaaaaag caacgctgac caatcaaagt tataatcttg
     2761 gcagaacctt ttcgctggca cttaattgta aattttaatt gtgtctgtca aattatatgg
     2821 aatcggcaaa ctgtttgaga attttgagtt tttcgaaacg tatggcatta tgattcctgt
     2881 aatacattct tcatatcttt tacatataac catcattgtt tcggaacaat cttgtaacag
     2941 tcaatcatta cttttgtcct gtaaaaaaat ttttagaata agccgagcat ctacggtaag
     3001 tttcaataag taaaatttaa tcaacacgtt tatgaaaagg aaatttttaa attatttcgt
     3061 tatttcttct gtcatggttt ccggtttaat tttctttacg gcttgtgatg acaagtcaga
     3121 tcccgatccg gaaccaaccc ctacttcaaa tgaagtaatc ttaaattcgg atatcaccgg
     3181 aacacgcaat ttggtaaacg attccgttta tattcttcaa ggacaggtaa atgtaagtgg
     3241 aaccatcaat attgaaaaag gaaccattat taaaggtgac aaattgacca aaggttgttt
     3301 ggttgttttg cgtggtggtg taatcaatgc tattggaact cccacagaac ctatagtttt
     3361 cacttcacgc atggaacccg gattacgcaa cgcaggtgat tggggtggaa tcattatagt
     3421 aggaaatgca ccggttaacc aatccaatcc aagtgtagaa ggtttgactc gggaagttac
     3481 ttacggtggt ggtacgaatc cagccgataa ttccggttca cttcgttatg ttcgcattga
     3541 atttgccgga atagccctgc agccagataa ggaaattaac ggactgactt tgtgtgcggt
     3601 aggaagtgga acaaccattg accatatcca agtttcctat tcaggagatg attctttcga
     3661 atggtttggt ggtacggtaa atgcaaaata tttgatagcc tattgcggat tggatgatga
     3721 atttgacacc gatttcggtt attcggggcg tgttcaattt gctttaggag ttcgtaatcc
     3781 ccgcgtagcc gatgtttcaa cttccaacgg tttcgaatcg gataacgatg gctctggtac
     3841 tggcgcaaca cctcaaactt ctgcagtatt ttcaaatgtt actctgatag gaccttacaa
     3901 aacaacagcc gataaaaatg taaacagcat gtttggagca ggaatgcata ttcgtcgcaa
     3961 cagctcaatt tcagtcttta attcggtttt tgcaggatgg aatacaggcc tgttactgga
     4021 tgggacatct acttatgcca atgcaactgc aagcaatttg gtaattcaaa attgcgtatt
     4081 gtccggtaca aaaggaacag ctgtgaacgg agcaggagga gtaactgctg atcaggctct
     4141 gacattcttc aacagcggga ataaccaaat cattgcagat aatgctacaa caggaattgc
     4201 cagttctttc tacgatgcaa ccaaaacagg cgctgaagct gccccttcct ctttcctccc
     4261 tgctgcaggt tcgccattgt tgagtggagc atcatttacc catgcaaaac tggctgacaa
     4321 tttcttcgac aaaactccaa cttttaaagg ggcctttggt acaacagatt ggacaaaaga
     4381 acaatggacc aattttgatc cacagaatac agtttattaa tcattttttc gtctgttttt
     4441 ttaagggcat tctaaaatcg ggatgccctt tttaattcta tgtaagtgtt ttaaacac
//
LOCUS       DAOSCH010000138         4491 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_12189_length_4491_cov_5.772543, whole genome shotgun sequence.
ACCESSION   DAOSCH010000138 DAOSCH010000000
VERSION     DAOSCH010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12189_length_4491_cov_5.772543"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLZ54_08335"
     CDS             <1..412
                     /locus_tag="PLZ54_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ66965.1"
                     /translation="VLLDEPTNGLDIPSKAQFRKMTASALEEERIMLISTHQVRDLDS
                     LIDSIIIMNSHQIVFNELCENITEKLLFSYGKEKSEGEEVLYSEENLRGFDQVSINKT
                     GEESKLDIELLFNTVLKNHQRIREIFSNQNNKRF"
     gene            425..1192
                     /locus_tag="PLZ54_08340"
     CDS             425..1192
                     /locus_tag="PLZ54_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66966.1"
                     /translation="MNQKFSLKRIGLLLRRYFIENQNYELYLWSILTIVFMIFRNGNS
                     ASAILIVTGALYAVRFFKVFNFAPNGIQYLLIPATQLEKTISVFLLNTVYFILMFTVV
                     FSVGNVIGNLLEQLIFSTSDTVALQFFQSSKGFEFNIQGLKNIWISFGVFAAIQAIFT
                     LGSLYFKRNAILKTLLSLFVVALALGILEYFIFKITFGSVTETLKALNAGDFVWDENI
                     FPKALTETWKIFWSISGYLFIPFCWIVSYFRLTEKEI"
     gene            1194..1568
                     /locus_tag="PLZ54_08345"
     CDS             1194..1568
                     /locus_tag="PLZ54_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOJ66967.1"
                     /translation="MEFNDAQAIYLQIADYISEHILLGKWKEGERLLSVRELGTELEV
                     NPNTVMRAYDFLQSNEIIYNKRGIGYFVADDAKEKILNYRRKRFLEKELPILFKNIHL
                     LNIDVEEIKKRYNDYLQKQQAL"
     gene            complement(1969..2622)
                     /gene="nth"
                     /locus_tag="PLZ54_08350"
     CDS             complement(1969..2622)
                     /gene="nth"
                     /locus_tag="PLZ54_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOJ66968.1"
                     /translation="MTTEERYLHILDWFMENMSNAQTELNYSNPFELLVAVILSAQCT
                     DKRVNMVTPQLFSDFPTPEAMASTTPEKLLEYIRSVSYPNSKAKHLVEMSRKLVADFE
                     GKVPDNVDKLQTLPGVGRKTANIIVSVIYNQPAMPVDTHVFRVSERLGLTTDSKNPLQ
                     TERELVRHIPPQLLPIAHHWLILHGRYVCHARKPKCNVCGLKSWCRYYFNHSLEQDI"
     gene            complement(2771..3223)
                     /locus_tag="PLZ54_08355"
     CDS             complement(2771..3223)
                     /locus_tag="PLZ54_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOJ66969.1"
                     /translation="MEQLFEKVSADIKTAMLAHDKVTLEALRGIKKEFLEAKTAKGSD
                     GTLSDEAAIKILQKMVKQRKETAQIYKEQNRPDLEQNELAEIAVIEKYLPAQMSESEL
                     EATLREIIAQTGATGPQDLGKVMGVATRQLVGKADGKAISEMVKKLLS"
     gene            complement(3275..4489)
                     /gene="ftsZ"
                     /locus_tag="PLZ54_08360"
     CDS             complement(3275..4489)
                     /gene="ftsZ"
                     /locus_tag="PLZ54_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOJ66970.1"
                     /translation="MKVIGVGGGGGNAVNHMYRQGITDVSFVVCNTDNQALVKSPVPL
                     KIQLGANTTSGLGAGGKPEVARQAAEESIEKIQNILQDNTKMVFITAGMGGGTGTGAS
                     PVIAKAAHDMGILTVGIVTIPFAFEGNLKIRQALEGVAALSEHVDAILVINNEKLKEI
                     YPDLELSNAFAKADDVLTNAAKGIAEIITIPGYINTDFADVYSIMKDGKVAIMNTGYA
                     SGENRITKAIENALNSPLLNTSDVSGASKVLLSLYCSSTHQIKMEEVQQIHDFMRKVG
                     ENVQVIWGATFDDELEDRVKITLIATGYDVSDIPGMPASVIKNKKRLETTPDKIEVFS
                     ELKPKENQPESKEKIIENAVNSYYGKSIPDEKPIEEPELDLPVVNLEDLENDQTLKYI
                     EKFPSWKRKKGK"
BASE COUNT         1355 a          887 c          823 g         1426 t
ORIGIN      
        1 agtattgctc gacgaaccga ccaacggttt ggatattcct tccaaagccc aattcagaaa
       61 aatgacggct tctgctttgg aagaagaacg cattatgctg atttccaccc atcaagttag
      121 agatttggac agcctgatcg acagcattat cattatgaac tctcaccaaa ttgttttcaa
      181 cgaactgtgc gaaaacatta ccgaaaaatt acttttcagt tacggaaaag aaaaaagtga
      241 aggtgaagaa gtgctttatt cagaagaaaa tttgcgcggc tttgatcagg ttagtatcaa
      301 caaaaccggt gaagaaagca aactggatat tgaactgctg tttaataccg tgctgaaaaa
      361 tcaccagcgc atcagagaaa tattttcgaa tcagaacaat aaacgttttt aaaaattctt
      421 caatatgaat cagaaattta gtcttaaaag aatcggattg ctcttgcgcc gatattttat
      481 cgaaaatcaa aattacgaac tctacctttg gagcattttg accattgtat ttatgatttt
      541 cagaaacgga aattctgcaa gcgccatttt aattgttaca ggtgcacttt atgcagtaag
      601 atttttcaaa gttttcaatt ttgcacccaa tggcattcaa tatctactga ttccggctac
      661 acaactggaa aaaacaattt ctgtgttttt gctcaatacc gtttacttta ttttaatgtt
      721 cacggttgtt ttttcggtag gaaacgtgat tggaaattta ctggaacaac ttattttcag
      781 tacaagcgat acggttgctt tgcaattttt tcaatcctca aaaggttttg agtttaatat
      841 tcaaggctta aaaaatattt ggatttcatt cggcgttttt gccgccatac aggctatttt
      901 cactttgggg tcgctgtatt tcaagcgaaa cgccatactc aaaaccttgc tgagtctgtt
      961 tgttgtagcc ctagcattgg gcatattgga atattttatt tttaaaatta cgtttggctc
     1021 cgttacagaa accctcaagg ccttgaatgc cggtgacttt gtatgggacg aaaacatctt
     1081 cccaaaagca ctaacggaga catggaaaat attttggtct atatcgggat atttgtttat
     1141 acccttttgc tggatagtaa gttacttccg tttaacagaa aaagaaattt aaaatggaat
     1201 ttaatgatgc acaagctatt taccttcaaa tagccgatta tatcagcgaa cacattttgc
     1261 tgggcaaatg gaaagaagga gaaagattgc tgtcggtaag ggaactgggt acagaattgg
     1321 aagtgaatcc caataccgtt atgcgagctt acgattttct gcaaagcaac gaaattattt
     1381 acaacaaaag aggaatcgga tattttgtgg ctgatgacgc aaaagaaaaa attctcaatt
     1441 atcgacgaaa acgatttttg gaaaaagaac tcccgatttt gtttaaaaac attcatctgc
     1501 tcaacatcga tgttgaggaa ataaaaaaaa gatataatga ctaccttcaa aaacaacaag
     1561 cattatgaaa acaattaaag aaatgaagac gagcaacaaa ttactggcgg tcttttttat
     1621 ctatataatt ataataatga ttatcaacaa tttaatctta aaaagtcaaa taaaccgcat
     1681 gttcgaatca acacaggaac aaaagacaga aaccggttta ggcagccatg atgcatcgaa
     1741 attgtaggga gtattttttt cagcaaaaac aaaaaaaagc aggttgtaaa ttcgagattt
     1801 tggaaaagaa aaattttcga aaaacttcaa aacaaagata taacgctctt attctttgaa
     1861 gccacaaaaa cttttaccgc taaaaattaa agcttaatac tgaaatatca ggctctttca
     1921 gaaaagatga caaaaacagt aaaattcgat tcaaattttt ctctcatttt aaatatcttg
     1981 ctctaatgaa tgattgaagt aataacggca ccatgacttt aagccacata cattgcattt
     2041 aggttttctg gcatgacaaa cataccgacc atgcaaaatt agccaatggt gagcaatggg
     2101 caacaattga ggcggaatgt ggcgaaccaa ttctcgttca gtctgcaatg gattttttga
     2161 atctgttgtc agtcccaagc gttcggaaac ccgaaacacg tgtgtatcga ctggcatggc
     2221 aggctgattg tagatgactg aaacaattat attggctgtc tttcttccca cacccggaag
     2281 cgtttgtagt ttgtccacat tatccggaac ttttccctca aagtcagcta ccaattttcg
     2341 cgacatttcc accaaatgct tagctttact gttgggataa gatacgctgc gaatatattc
     2401 caataacttt tcaggtgtgg ttgaggccat agcttctggc gtgggaaaat cggagaaaag
     2461 ttgaggcgta accatattta cccgtttatc agtacattgt gccgataaaa ttacagctac
     2521 caataactcg aaagggttag aatagtttaa ctccgtttga gcattggaca tattttccat
     2581 aaaccaatct aaaatgtgca aataacgttc ttcagtggtc atgaaataaa atcaatagtt
     2641 tagaaaaatt ctctttttat tgagaacaaa aaacgctatg aaacccacag gtattgcata
     2701 cacaaacacg aatgaatata atattcagac atgtgggttc catcaaacat taaaaaaata
     2761 aattatattc ttatgaaagc aattttttta ccatttccga aatcgctttc ccatctgctt
     2821 ttcccaccag ctgtcgagtg gcaactccca ttacttttcc caaatcctga ggccctgtgg
     2881 caccggtttg agcaatgatt tccctcagag ttgcttccag ttcgctttcg ctcatttgag
     2941 ccggcagata tttttcaatg acggcaattt ctgccaattc attttgctcg agatcgggac
     3001 gattttgttc tttgtaaatc tgcgctgttt ccttgcgctg ctttaccatt ttctgcaaaa
     3061 tttttatggc tgcttcatcc gataatgttc catcacttcc tttggcagtc ttggcttcca
     3121 gaaattcctt tttaattccc cgaagggctt ccaacgtaac tttatcgtgc gccagcatgg
     3181 cagtctttat gtcggcactt accttttcaa atagctgttc catttgtatt ttaaattagt
     3241 agatttgtct tttttaattc tcggaaaaac acttttattt tccctttttt ctcttccaag
     3301 aaggaaattt ttcaatgtat ttcaaagttt gatcattttc caaatcttcc aggttcacta
     3361 ccggcaagtc caattccggt tcttctatag gcttttcatc gggaatggat tttccataat
     3421 agctgtttac ggcattttca ataatttttt ccttgctttc cggttgattt tctttgggtt
     3481 ttaattcgga gaaaacttca attttgtcag gagtagtctc taaccttttt ttatttttaa
     3541 taaccgatgc aggcatgccg ggtatatcgc tcacatcata accagtggca ataagggtta
     3601 ttttaactcg atcttccaat tcgtcatcaa aagttgcgcc ccagattact tgaacgtttt
     3661 cgccaacttt tcgcatgaaa tcatggattt gttgtacttc ctccatctta atttgatgag
     3721 tcgaagagca atacaagctc aagagaactt tagaagctcc gctcacatca ctggtattga
     3781 gcaatgggga gttaagcgca ttttcaatgg ctttggtgat gcggttttca cccgaagcat
     3841 aaccggtgtt cataatggct actttgccat ctttcatgat actgtataca tcggcaaagt
     3901 ctgtgttgat ataacccggg atggtaatga tttctgctat tccctttgct gcattggtca
     3961 gcacgtcgtc agctttggca aaagcattgg acagttctaa gtcgggatag atttctttca
     4021 atttttcgtt gttgattacc agaatagcat ctacatgttc gctcaaagct gctacacctt
     4081 ccaatgcctg cctgattttc agattccctt caaaggcaaa aggaatggta acgataccaa
     4141 cagtaagtat tcccatatcg tgagctgctt tggcaattac cggagaagct cctgttcctg
     4201 tgcctccacc cataccggca gtgataaata ccatttttgt attgtcctgc aaaatgtttt
     4261 gaattttttc gatggattct tcggctgcct gacgtgcaac ttcaggtttt ccgccggctc
     4321 caagccctga tgtggtattg gcacccaact gaattttgag aggaacaggc gattttacca
     4381 aagcttgatt gtcggtgttg caaacaacga acgacacatc cgtgattccc tgacgataca
     4441 tgtggttgac ggcatttcct ccgccacccc ctacaccaat tactttgata a
//
LOCUS       DAOSCH010000139         4312 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_12852_length_4312_cov_8.757811, whole genome shotgun sequence.
ACCESSION   DAOSCH010000139 DAOSCH010000000
VERSION     DAOSCH010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12852_length_4312_cov_8.757811"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="PLZ54_08365"
     CDS             <1..845
                     /locus_tag="PLZ54_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOJ66971.1"
                     /translation="FYFLEKKDVIPFLPERKKFSHKGTYGHALIVAGSVGMAGAAVLS
                     AQAAIRSGTGLVSVHSAATNRIILQTKVPEALFESDKEENFISELKNLEKYNTLAIGP
                     GIGTQEKTANFLFQILPSIKQSMVLDADALNIMSRKKELLEKLSHNTLLTPHPKEFER
                     LFGADKNSYDRLLKMQQLAKNLHLVIILKGAYTRIAFPDGSIYFNSTGNPGMATGGTG
                     DVLTGILAGLLAQNLSPEKAALLGVYLHGLSGDLALRHQSMESLTAGDVIENLGSAFQ
                     SLRE"
     gene            973..>1223
                     /locus_tag="PLZ54_08370"
     CDS             973..>1223
                     /locus_tag="PLZ54_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HOJ66972.1"
                     /translation="MTKKILIFLFLSFSIVTFGQTLNLDTCQKRAKENYPLLKQYGLI
                     EKTADYNISNANKAYLPQVSLLAKATYQSDVTAIPETLG"
     assembly_gap    1224..1323
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1324..2099
                     /locus_tag="PLZ54_08375"
     CDS             <1324..2099
                     /locus_tag="PLZ54_08375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOJ66973.1"
                     /translation="ELASNYQRMEAYLKNGLIQQADLDAVKVEQINAAQQETDLKSVL
                     KSYCQMLSVFTGMTVNEKTELEKPLLPVVNENIENKRPELSLFESQKNLLESQKKTIL
                     AGNLPKIGLFIQGGYGRPGFDFFNNDFTSFYIGGVRLSWNIAGFYTQKNELKKIETSQ
                     KNVDIMKETFLFNANLLTKQQMNEIDKLRVTLKNDEEIIRLRNNIKKATESKFQNGTI
                     TTTDFLREINAENIARQTRTLHEIQLYMAVYQLKNTTNN"
     gene            2122..3045
                     /locus_tag="PLZ54_08380"
     CDS             2122..3045
                     /locus_tag="PLZ54_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005788227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOJ66974.1"
                     /translation="MKALIFDKKNLVLMSLIVGLTSCHRLLENKYDASGTFEATEVVV
                     SAEANGKIMDFNLEEGQQLTENQEVGYIDSTQLYLTKLQLLASQKSVQSRVPDIKKQI
                     AVLQQQIETAKKEQKRIQNLFSANAASQKQLDDVNAQVSVLEKQLVAAKSNLESTSNS
                     ILNEKEALNIQIEQIQNQLEKCRITSPINGTVLVKYAEKGELAGAGKPLFKVGDLQKM
                     ILRAYVTADQVTQLKIGQKAKVFVDFGKDDQKEYEGTVGWISGESEFTPKTIQTRDER
                     ANLVYAVKINVKSDGLLKIGQYADVKFIAEN"
     gene            3088..4032
                     /locus_tag="PLZ54_08385"
     CDS             3088..4032
                     /locus_tag="PLZ54_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ66975.1"
                     /translation="MTGNYGEFAVCCENITKTYPPDIQALKNIDLKVSPHQIFGIIGP
                     DGAGKTTLFRMLTTLILADEGKATVLGYDVVNDYQEIRKYVGYMPGRFSLYQDLTVEE
                     NLKFFASVFGTTVEENYDLIKNIYRQIEPFKNRRAGQLSGGMKQKLALSCALIHKPKL
                     LILDEPTTGVDPVSRKEFWEMLQNLKNEGITILVSTAYMDEAGLCDTIALILNGKFLK
                     IDTPQNIVQQFEPAIYSVQSNQRSKLLNDLRSLPQVSSCYAFGESIHVTVENEKISKE
                     ELQNYLLKSGHEQIEVAKIAPTVEDCFMELSKQQEQNN"
     gene            4035..>4312
                     /locus_tag="PLZ54_08390"
     CDS             4035..>4312
                     /locus_tag="PLZ54_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOJ66976.1"
                     /translation="MEKVIEVQNLTKKFGDFTAVDHISFEVYKGEIFGFLGANGAGKT
                     TAMRMLCGLLPPTSGKGLVAGFDIHTQSEEVKRNIGYMSQKFALYEDL"
BASE COUNT         1473 a          710 c          844 g         1185 t
ORIGIN      
        1 atttttattt tttagaaaaa aaagatgtaa ttccgttttt accggaaaga aaaaaatttt
       61 cccacaaagg tacttacgga catgcattga tagtggctgg aagtgtgggg atggctggag
      121 ctgccgtttt atcggctcaa gctgccatta gaagtggaac gggattggta agcgtgcatt
      181 cggctgcaac aaacagaatt attcttcaga caaaagttcc ggaagctttg tttgaaagcg
      241 ataaggaaga aaattttatt tcagaattaa aaaacttgga gaaatacaac actttggcca
      301 ttggtccagg tattggcact caagaaaaaa cagccaattt tttgttccaa attcttcctt
      361 caatcaaaca atcgatggtt ctggatgctg atgccttgaa tattatgagc agaaaaaaag
      421 aattgttgga aaaactttct cacaataccc ttcttacgcc acatcccaaa gaatttgaaa
      481 gactttttgg tgcggataaa aattcttatg atcgtttatt gaaaatgcag caactggcaa
      541 aaaatttgca tctggtcatt atcctgaaag gagcctatac gcgaattgcc tttcctgacg
      601 gcagtatcta ttttaacagc accggcaatc caggaatggc tacaggagga acaggtgacg
      661 ttttgacagg aatactggca ggtttactcg ctcaaaacct ttctcccgaa aaggcagcac
      721 ttttgggcgt ttatttgcat gggctttccg gtgatttggc tttacgacat caaagtatgg
      781 aatctttaac tgcaggcgat gtaatagaaa atttgggaag tgcttttcaa tctttaagag
      841 agtaatatag ttttgatata tttttttgta tatcaaaaaa tcaactcgaa aaatttagat
      901 ttttaaatat aagcataaca aataattcac agaaaaaact tttttcaaac cctcataaaa
      961 agtaaaagta ttatgacaaa gaaaatattg atttttctgt ttttatcttt ttcgatagtt
     1021 acgtttggtc aaaccctaaa tcttgatact tgccagaaaa gagctaaaga aaattatccc
     1081 ttgttgaaac aatatggatt gatagaaaaa acggccgatt acaatatttc caatgctaac
     1141 aaagcttatt taccacaagt cagcttgctg gcaaaagcta cttatcagtc agatgtaacc
     1201 gctattcccg aaacacttgg tcannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnaagaatt ggcaagcaat tatcagcgaa tggaggctta tttaaaaaac ggattaatac
     1381 aacaagccga cttggatgct gtgaaagtgg aacaaatcaa tgctgcacaa caggaaaccg
     1441 atttaaaaag tgttctgaaa tcgtattgtc aaatgttgtc ggtgtttacc ggcatgactg
     1501 tgaatgaaaa aacagagctt gaaaaaccac ttttacctgt tgtaaatgag aatattgaaa
     1561 ataaacgtcc ggaactttcg ctttttgaat cacagaaaaa tttattggaa agtcaaaaga
     1621 aaaccatttt agctggaaac ttgcctaaaa taggattatt tattcaaggt ggctacgggc
     1681 gaccgggatt tgattttttc aacaatgatt ttacttcctt ttatatcgga ggtgtgagac
     1741 tttcatggaa tatagccgga ttttatacac agaaaaatga attgaaaaaa atcgaaacca
     1801 gtcagaaaaa tgtggatatt atgaaagaaa cttttctttt caatgccaac ctgcttacca
     1861 agcaacaaat gaacgaaata gacaagcttc gtgtgacatt gaaaaacgat gaagaaatta
     1921 tacgtttgcg gaacaatata aaaaaagcta cggaatcaaa atttcaaaat ggaacaatta
     1981 ccacaaccga ttttttacgt gaaataaatg cagaaaatat cgcacgtcag accagaacac
     2041 ttcacgaaat tcaactatat atggctgttt atcaattgaa aaatacaaca aataattaaa
     2101 atttgaaacc atctgaaaaa tatgaaagct ctaatttttg acaaaaagaa tttggtgtta
     2161 atgagtttga ttgttggatt aacatcgtgt catagacttt tggaaaacaa atacgatgct
     2221 tccggaactt ttgaagctac ggaagtggtc gtatcagctg aagctaacgg taaaataatg
     2281 gatttcaacc tcgaagaagg tcaacagttg accgaaaatc aggaggttgg ctatatagac
     2341 agtacgcaac tttatctgac aaaattacaa ttactggctt cgcaaaaatc tgttcagagt
     2401 cgagttccgg atattaaaaa gcaaattgcc gttcttcagc aacaaataga aacagcgaag
     2461 aaagaacaaa aacggattca gaatctattc agcgccaatg ctgccagcca aaagcaactc
     2521 gacgatgtca atgcgcaagt ttcggtgctc gaaaaacagc ttgtggctgc aaaatcgaat
     2581 cttgaatcga ccagtaatag tattttgaat gaaaaagaag ctttgaatat tcagatagag
     2641 caaatacaaa atcaattgga aaaatgcaga attaccagtc cgattaatgg aaccgtgctg
     2701 gtgaaatatg ccgaaaaagg ggaattggct ggtgccggca aaccgctttt taaagttggc
     2761 gacctgcaaa aaatgattct gcgagcttat gttacagccg atcaggttac tcaactgaaa
     2821 attggtcaaa aagcaaaagt atttgtcgat tttggcaaag atgatcaaaa agaatatgaa
     2881 ggaacagtcg gttggatttc cggcgaatca gaatttacac ccaaaaccat tcaaacgcgc
     2941 gacgaaagag ccaatttggt ttatgccgta aaaatcaacg taaaaagtga tggtttgctg
     3001 aaaatcggtc agtatgctga tgttaaattt attgccgaaa attaatgatt aataattttt
     3061 gatattttcc ttaattgaaa atcgagaatg acaggaaatt acggagaatt tgcggtttgt
     3121 tgtgagaaca ttacaaaaac ttatccaccc gatattcagg cgctaaaaaa tatagattta
     3181 aaagtatctc cccatcagat atttggtatt attggtcctg atggagcggg aaaaaccacc
     3241 ttgtttcgta tgctcacaac gctaattttg gctgacgaag gaaaagcgac ggtgcttggc
     3301 tatgatgtgg tgaatgatta tcaggaaatc agaaaatatg ttggttacat gccaggtcgt
     3361 ttttcgcttt atcaggattt gacggtagaa gaaaatttaa aattttttgc ttccgttttc
     3421 ggaaccacag tagaagaaaa ttacgattta attaaaaata tttatcggca aatcgaacct
     3481 tttaaaaacc gaagagccgg ccaactttcc ggtggaatga agcaaaaatt ggcgttgagt
     3541 tgtgcgctta ttcacaagcc aaaacttttg attctggatg aacccacaac cggggtggat
     3601 ccggtttcgc gaaaggaatt ttgggaaatg cttcaaaatc tgaaaaatga aggaataact
     3661 atattggttt ccaccgctta tatggacgaa gccggtcttt gcgataccat tgccctgatt
     3721 ttaaatggaa aatttttgaa aattgatacc ccacaaaata ttgtgcaaca attcgagccg
     3781 gcaatatatt cggttcaaag caatcagcgt tcgaaactgc tgaacgattt acgcagcttg
     3841 ccacaggttt cgagttgcta tgcttttggc gaaagtattc acgtaacggt tgaaaacgaa
     3901 aaaatttcca aagaagaatt gcaaaattat cttttaaaaa gcggacatga acaaattgaa
     3961 gttgctaaaa tagcgccaac cgtcgaagat tgttttatgg aactttccaa acagcaggaa
     4021 caaaataatt gagtatggaa aaggttattg aagtacaaaa tttgactaaa aaatttggcg
     4081 attttacggc cgtcgatcat atttcctttg aagtgtacaa gggggaaatt tttggttttc
     4141 ttggagccaa cggagccggc aaaaccactg ccatgcgcat gttgtgcgga cttttgccac
     4201 caacttccgg aaaaggactc gttgccggtt ttgatattca cacccaatcg gaagaggtga
     4261 aaagaaacat cggctacatg agccaaaaat ttgctttgta cgaggatttg aa
//
LOCUS       DAOSCH010000140         4179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_13406_length_4179_cov_8.524248, whole genome shotgun sequence.
ACCESSION   DAOSCH010000140 DAOSCH010000000
VERSION     DAOSCH010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13406_length_4179_cov_8.524248"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="PLZ54_08395"
     CDS             <1..1291
                     /locus_tag="PLZ54_08395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HOJ66977.1"
                     /translation="LIASQQGIHINAMGIDTWGVDFVYLANDGSLLNLPRSYRDPYTN
                     GVPEEYFSRISKREVYELTGIQIMNLNSLFQLYAAKKESSSALEYADSILFMPDALNY
                     LFTKKKVCEYTIASTSQLLNPFTKKIEAELLVPMNIEPTILPQMVMPGTVVGNLEKSI
                     QRECNVPEIPFIAVAEHDTASAVVAVPAEDENFAYLSSGTWSLMGIETKNPIITEESY
                     RLNFTNEGGVDGTIRFLKNITGMWLLERCREEWKLDGNSYTYPEIVQMAESVRSFQFL
                     IDPDDSIFSNPESMTQAITVYCNNSGQKVPITHAELIRCIFDSLALKYKYVLNSLQKL
                     SSFQINRLHVIGGGSQNKLLNQLTANAIGLPVIAGPAEATAMGNIMMQAKGLGLINSL
                     TEIRSIIRNSVELETFYPQNTELWEKAYKKYLHILEK"
     gene            1325..2584
                     /locus_tag="PLZ54_08400"
     CDS             1325..2584
                     /locus_tag="PLZ54_08400"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HOJ66978.1"
                     /translation="MKKDEIIQKSYEIAKERYEAVEVSVDKAMEVLEKLSLSIHCWQA
                     DDVTGFENFGNINDAGIQATGNYPGKARNMDELRQDIEKVLALVGGKHRLNLHAIYGD
                     FQGKKIDRNEIEPSHFTSWMQWAQEKGLKLDFNSTSFGHPKSGNLTLANPDKEIRNFW
                     IEHTKRCRLIAEEMGKFQNDPCIMNLWIHDGMKDLTVNRLLYRSILEKSLDEIFAKGY
                     TNMKDSIEAKLFGIGLESYTVGSYDFYLAYGTKNQKIVTLDTGHFHLTESVADKISSL
                     LLFVPEIMLHVSRPVRWDSDHVTILNDDVTELAKEIVRADALSRVHVGLDYFDASINR
                     IGAYVVGIRATQKAFFQALLEPVSLLRKYEDNGQYFERLALLEETKNLPWNAVWDYFC
                     LKNNVPVGEDYIAEIQKYEKNVTSKRN"
     gene            2721..3755
                     /gene="rhaT"
                     /locus_tag="PLZ54_08405"
     CDS             2721..3755
                     /gene="rhaT"
                     /locus_tag="PLZ54_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HOJ66979.1"
                     /translation="MNTILGLIIIAIGSIGQSSFYVPIKKVKNWSWESFWLMQGIFAW
                     LVFPFIGSLMAIPAGHSLGEIWSAGGSDMWKSVGYGILWGIGGLTFGLSMRFLGIALG
                     QSLALGTCSAFGTLIPALLKGDNLLKGDGLILLIGVCLAIAGIAVIGYAGSLKAKNLT
                     EEERKKAVKDFALKKGILIALLAGIMSACFNLGLESGSSIKTALLNIGGNSLMVLNPV
                     IFLVTIGGFLTNAVYCFYQNYKNQTFSDYTQSSGKIWMNNLLFCALAGVLWYSQFFGL
                     GAGQSFFAPGSVMMAFSWSILMSLNVLFSNIWGIILKEWNGVGKKTIFVLIIGLAILI
                     FSIIFPQIVK"
BASE COUNT         1320 a          633 c          848 g         1378 t
ORIGIN      
        1 tttaattgca agtcagcaag gaatccatat caatgcaatg ggtatcgata catggggagt
       61 tgattttgtg tatctggcaa acgatggttc tttacttaat cttccgcgtt cctatcgtga
      121 tccatatact aacggagtac cggaagaata tttttcacgt atttcaaaga gagaagttta
      181 tgaactgaca ggcattcaga ttatgaattt aaacagcttg tttcaattgt atgcagcaaa
      241 aaaagaatct tcttcagctc ttgaatatgc tgattcaatt ttgtttatgc ccgatgctct
      301 taattatttg tttaccaaga aaaaagtttg cgaatatacc attgcttcaa cttctcaatt
      361 gttaaatcca ttcacaaaaa aaattgaagc cgaattatta gttccaatga atattgaacc
      421 aacaatacta ccacaaatgg ttatgccggg tacagttgtt ggaaatttgg aaaaaagtat
      481 tcaacgggaa tgcaatgttc ctgaaattcc cttcatcgca gttgcagagc atgatactgc
      541 ttcggcagtg gttgcagtgc cggcagagga cgaaaatttt gcctacctga gttcggggac
      601 atggtcatta atgggcattg aaaccaaaaa tcccattatt acagaagaaa gttatcgttt
      661 aaattttacc aatgaaggtg gagtagatgg taccatacga ttcttgaaaa acataacagg
      721 catgtggctt ttggaaagat gccgcgaaga atggaaattg gatgggaatt cctatacgta
      781 tcctgaaatt gttcaaatgg cagaatcagt aaggagtttt caatttttaa ttgatcctga
      841 tgacagtatt ttttcaaatc ctgaaagcat gacacaggca ataactgttt attgtaacaa
      901 tagtggacaa aaagttccta ttacacatgc tgaattgatc cgttgtatct ttgacagttt
      961 ggcattgaaa tataaatatg ttctaaattc attgcaaaaa ttgtcttctt tccaaataaa
     1021 tcgacttcac gttattggag gtggctctca aaataaattg cttaatcagc taacagctaa
     1081 tgctattgga cttcctgtga ttgcaggacc tgcagaagct actgcgatgg gaaacattat
     1141 gatgcaggca aaaggattgg ggctgatcaa ttcattaact gaaattaggt ctatcatccg
     1201 aaattctgtt gagctggaaa ctttttatcc tcaaaataca gagttatggg aaaaagcata
     1261 caagaaatat cttcatattt tagaaaaata aaagtcgaaa taactattac aaaattaatg
     1321 tatcatgaaa aaggatgaaa ttattcaaaa gtcctacgaa attgcaaaag aacgttatga
     1381 agcagtagaa gtaagtgttg acaaagcaat ggaagttctc gaaaaattat ctctttccat
     1441 tcactgctgg caagccgatg atgtgacggg ttttgaaaat tttggcaata tcaacgatgc
     1501 aggtattcaa gccactggca attatccggg aaaagcacgc aatatggatg aacttcgtca
     1561 agatatagaa aaagtgttgg cattggtagg aggtaaacat cgcttaaatc tccatgctat
     1621 ttacggagat tttcaaggga aaaaaattga ccgcaacgaa atagagcctt cccatttcac
     1681 cagctggatg caatgggctc aagagaaagg gctaaaactt gattttaatt cgacatcttt
     1741 tggacatcca aaaagtggta acctcacgtt ggcaaatccc gacaaagaaa ttcgcaattt
     1801 ttggattgaa cacaccaaac gttgtcgact aattgccgaa gaaatgggaa aatttcaaaa
     1861 cgacccttgt attatgaatc tttggattca cgacgggatg aaagatttaa ctgtaaatcg
     1921 gttgctttac agaagcattt tggaaaaatc gttggatgaa atttttgcca aaggttacac
     1981 aaacatgaaa gacagtatag aagcaaaact ttttggtatt gggttggaaa gttacacggt
     2041 aggttcatat gatttttatc tggcttatgg tactaaaaac caaaaaatag ttacgttgga
     2101 tacaggccat tttcatttga cggaaagtgt tgctgataaa atatcttctc ttttgctttt
     2161 tgttccggaa attatgttgc atgtaagtcg gccggtgcgg tgggattccg accatgtaac
     2221 gatattaaat gatgatgtta ctgaattggc taaagaaatt gtacgggcag atgccttgag
     2281 tcgagttcat gtggggttgg attatttcga tgcttctatc aatcgcatcg gagcgtatgt
     2341 tgttggcata cgggctacgc agaaagcttt ttttcaggcc ttgctagaac ctgtttcttt
     2401 attgagaaaa tatgaagata atggacaata ttttgaacgg ctggcattgc tcgaagaaac
     2461 caaaaatttg ccctggaatg ctgtttggga ctatttttgc ttgaaaaaca atgtgcctgt
     2521 cggagaagat tatattgctg aaattcaaaa atatgagaaa aatgtaacct caaaacgaaa
     2581 ttaaatttca tcagaatata atttattttt taaaggtctg atggaaccca catgtctgaa
     2641 tattatattc attcgtgttt gtgtatgcaa tacatgtggg tttcttgatt aaaaattttt
     2701 taaaagccat tttttatatc atgaatacca tcttaggatt aataattatt gccatcggta
     2761 gtattggaca gtcaagtttc tatgtcccca taaaaaaggt caaaaattgg tcgtgggaaa
     2821 gtttctggct gatgcaaggt atttttgcat ggcttgtttt cccatttata ggctcattaa
     2881 tggcaattcc tgccggacat tcgctgggag aaatatggtc tgcgggtggt agtgatatgt
     2941 ggaaatctgt tggatatggt attctttggg gaattggagg cttgaccttt ggtcttagta
     3001 tgcgtttttt gggaattgcg ttaggacagt cattagcttt gggaacatgc tccgcattcg
     3061 gaacattgat accggcttta ttaaaaggtg ataatctgtt gaaaggcgat ggattgattc
     3121 tgttaatagg agtttgtctt gctattgccg gtattgcagt gattggttat gccggttcgc
     3181 taaaagcgaa aaatttaacc gaagaagaaa gaaaaaaggc agtaaaagat tttgctttga
     3241 aaaaaggaat tctcattgct ttgctggctg gtattatgag tgcatgtttt aatttgggtt
     3301 tggaatccgg aagctctata aaaactgctc ttctgaatat tggagggaac agtttaatgg
     3361 tactgaatcc ggtgattttt ctggttacta ttggtggttt tctaacaaat gcagtttatt
     3421 gtttttatca aaattataaa aatcaaactt tttccgatta cactcaatct tcagggaaaa
     3481 tctggatgaa taatttgttg ttttgcgcct tggcaggcgt tctctggtat tcgcagtttt
     3541 tcggattggg tgccggtcaa agtttttttg ctcccggaag tgtcatgatg gcattttcat
     3601 ggagtattct tatgtcactg aatgtgttgt ttagcaatat ttggggaatt atattgaaag
     3661 aatggaatgg cgttggtaaa aaaacaattt ttgttcttat tattgggttg gccatattga
     3721 tattctctat tatatttccc caaattgtaa aataaacatg taatttatcg caaatggaat
     3781 tgctcatcaa taggtacttg acggttgcac caagcaattg agtcagaaac aagcataaga
     3841 ataggtatcc ttctacctcc gaatttacta caaatgtttt aataaaatta atttgacaga
     3901 tatgttatta acttctcaca catttcgacg gaaagtgaat tcttcaaaat tttttcataa
     3961 ttttgcattt gctggttgaa tctactatta atgttttgta aaaaaatatt ttgaagtttt
     4021 gataacaatc cttattctta ggctattttg aaattttatt tagaaaacat caatttcata
     4081 atagatttat tttctggtaa catgcgtttc taaataaaaa attatttttc ttttatctaa
     4141 ttttacttta cttattaagt caaattcttt caaaaggaa
//
LOCUS       DAOSCH010000141         4152 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_13526_length_4152_cov_5.540639, whole genome shotgun sequence.
ACCESSION   DAOSCH010000141 DAOSCH010000000
VERSION     DAOSCH010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13526_length_4152_cov_5.540639"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..525
                     /locus_tag="PLZ54_08410"
     CDS             82..525
                     /locus_tag="PLZ54_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOJ66980.1"
                     /translation="MCAPGYDIYSTATSNTYFTSNGTSFAAPQVAGVAAMIIAVNPSL
                     SSNQVVDILKSTADTTIYDIPENTNYIGQLGSGRLNAYKAVKKACSLDFNNTAFVGNS
                     NETGCYITATNCIIESTANVVFDATKEVNLQSGFEVQLGSTFEAK"
     gene            877..1305
                     /locus_tag="PLZ54_08415"
     CDS             877..1305
                     /locus_tag="PLZ54_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66981.1"
                     /translation="MASTSETGHAKNVANFNELISFVSGYGETYNPSKASIKLTALQT
                     LLADAKSAMDAVNSAMPAYSNAVSAREAAFEPLNKLITRVMNAVKATDISSQVEESVK
                     TLVRKIQGTRSTAKKTETQKADMTAEGKEVKEISTLQDVL"
     gene            1828..2001
                     /locus_tag="PLZ54_08420"
     CDS             1828..2001
                     /locus_tag="PLZ54_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66982.1"
                     /translation="MLPEVSNAVNTLNTSGSVIVKSTDRITFEAGQEIILNTGFEVQQ
                     GGQLDVNIVTCGQ"
     gene            2019..2729
                     /locus_tag="PLZ54_08425"
     CDS             2019..2729
                     /locus_tag="PLZ54_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ66983.1"
                     /translation="MKRILIVLVFNLLFSHFLFAQENSRINSFFNDTLKGKWILESNC
                     LLEYQRCDSIGKGIEYIIFNSINSQGFVKVVTNGSWSFENGDSTKLYLPEKTIFGETW
                     VMDDYIFYSYNKICVINENIDKLFLSTNSFIGFAKSYIRDKSYLGLQSKINIPITIYP
                     NPFIDKINVSYDIPNSTNRVLINIYSSTGLLLKKIEIAENGQFKKTISLEDLNKGIYF
                     GVVSLNDKWINNIKLIKK"
     gene            complement(3013..4077)
                     /gene="buk"
                     /locus_tag="PLZ54_08430"
     CDS             complement(3013..4077)
                     /gene="buk"
                     /locus_tag="PLZ54_08430"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOJ66984.1"
                     /translation="MFKILSINPGSTSTKFAVYEDEKILFLHTILHSTEELKSFEKII
                     DQLDFRKNLILNSLNHDGVDLRTLSAVVGRGGLIRPVASGVYLVNERMIADLREAKRG
                     EHASNLGGIIAYEISRDIPSCKPFIVDPVVVDEMQDVARISGLPQFPRRSMFHALNQK
                     AVARKYAASAGTGYENLNLVVAHLGGGISVSAHAKGKVIDTNQGLGGYGPFSPERSGT
                     IDAGLLVDYCFQSQISKEEIKKMLVGRGGLIAHLGTNDAYLAEKKALNGDAYAKLIME
                     AMAYNVAKEIGAMCAVLKGKVDAIILTGGISRSEWFVNYVKEMVSSFGKIVVFPGENE
                     VEALAMGALRVLRGESYKEY"
BASE COUNT         1416 a          756 c          706 g         1274 t
ORIGIN      
        1 aattgcaaaa aattggaaag atgtacatga aagatacatt ggggatccaa gtagacacat
       61 aatcataatg aagctgttga tatatgtgca ccaggatatg atatatactc gactgcgaca
      121 tccaatacat attttacttc aaatgggaca tcttttgctg ctccacaagt agctggtgtt
      181 gcagctatga taattgctgt aaatcctagt ctttcatcaa atcaggtagt agacatatta
      241 aaatctacag cagatacaac aatctatgat attcctgaaa acacaaatta tattggtcag
      301 ctaggatcag gtagattaaa tgcttataaa gctgtaaaga aagcatgttc tttagatttt
      361 aataatactg cttttgtagg taatagtaat gaaacaggtt gttacattac agcaactaat
      421 tgcataattg aatctacagc aaatgtagtt tttgatgcaa caaaggaggt taatttacaa
      481 agcggttttg aagttcaatt aggtagtact ttcgaagcta aataattatt ctttatgtag
      541 gagtttaatc gccccaaaag caaagaaaaa aaaccacagc aagcgagaat atatattcaa
      601 aagtcttgct gtggctttta tagctttttg ataataggca gatcggtcac agtacattgt
      661 ttattgctcg tttagacaat tgaactgcat tttgaaagaa aaaacaatga actgtgaagt
      721 atttattgct tagcaatact taataggtta attctcatag ataaaaaatg tgaaaaatgt
      781 ttgtaaatta cctacaaaat aaatcttaaa tacaatctat ttaactatat ttgcagcata
      841 attatttcct tttataaatc taaaatctaa cttattatgg caagtacttc agaaacagga
      901 cacgcaaaaa acgtggcaaa cttcaacgaa ctgatatcgt ttgtttcggg ttacggagaa
      961 acctacaatc cctcaaaagc ctccataaaa ttaaccgcct tgcaaaccct cctggcagat
     1021 gcaaaatccg ctatggatgc ggtgaactca gcaatgcccg cttacagcaa cgccgtttcg
     1081 gcacgtgaag ccgcctttga accattaaac aaactcatca cccgagtaat gaacgccgtt
     1141 aaagccacag atatctcttc ccaagtagaa gaaagcgtaa aaacgttggt tcgaaaaatt
     1201 cagggaacaa gatccactgc aaagaaaaca gaaacgcaaa aagcggacat gacagccgaa
     1261 ggaaaagaag tcaaagaaat atccacccta caggatgttc tttaaataac taaagttggc
     1321 gttcgtcgct gggatttgca atcccagctt cttgtattat aaggatttat aatccgctaa
     1381 gttaaatatt tagattaaaa agttatcttt gctcaataaa tatattttgc cgtggaaagt
     1441 gtttcggatt acaaaccttt ataatacatt acgaggtgat tgcaattatt ctcttctgtc
     1501 atgaccgttg gttcacctca gacgtccgca aagctttgta tattgaatga ggatattcag
     1561 taggacgggg ttatctcccg cattatcttt tgtgttgttc gacagtgaat acgcccacca
     1621 ttcggctatt gcgtttagcc tcggtcgata tcaagaaact tatcattcga tatcaagcat
     1681 aaactcgaaa atgatttacg caggtttgat attttgtatt taagaatatt gtatatttgt
     1741 tgcgtaaatg agacgttacc tgtaattttt ttaacttaaa acaaatattt atgctcaaac
     1801 acagatatta cattatcaaa cacaaatatg ctacccgaag tttcaaatgc agttaacaca
     1861 ttaaatactt caggttcggt tattgttaag tcaaccgata gaattacatt tgaagctggg
     1921 caagaaataa tattgaatac cggatttgaa gttcaacaag gaggtcaact tgatgtaaat
     1981 attgtaacat gtggtcaata atctaaatct aaaaaaacat gaaaagaatt cttattgtat
     2041 tagtctttaa cctattgttt tcacattttc tttttgctca ggaaaacagt agaataaact
     2101 catttttcaa cgatactttg aagggaaaat ggattttaga aagtaattgc cttttagaat
     2161 atcaaagatg tgattctatt ggaaaaggaa ttgagtatat aatattcaat tcaataaatt
     2221 cacagggttt tgtaaaagtt gtaacaaatg gttcttggag ttttgagaat ggagattcta
     2281 caaaattata tcttcctgaa aaaacaattt ttggcgaaac atgggtcatg gacgattata
     2341 ttttttattc ttataacaaa atatgtgtta taaatgagaa tattgacaaa ttattcctat
     2401 ctactaacag ttttattgga tttgcaaaat catatatccg tgacaaatct tatttagggt
     2461 tgcaatctaa aataaatata ccaataacaa tttatcccaa tccatttatc gataagatta
     2521 atgtttctta tgatatccca aattcaacga atagagtatt gataaacata tattcctcta
     2581 caggattatt actaaaaaaa atcgaaattg cagaaaatgg acagtttaag aaaacaatct
     2641 cattggagga tttaaataaa ggaatttatt ttggagttgt ttctctaaat gataaatgga
     2701 ttaacaacat aaaactaatt aaaaagtaaa ttcaaaaaaa tcaaataaga caaaacgaac
     2761 aagaaataga aacaattttc aaactttggt aacacacgcc taagcacaat agccgaattc
     2821 gtgcgatttt ggaggtttat ctctcgcata agtttttgtg ttgttcgaca gtgaatatgc
     2881 ctctgctgcc gcacgattgc atcgtgtggc agcgtggggt ttggtgggaa gaaaaatttg
     2941 caactgtaaa agaaaaaaat aaaaactgat tggtttccca atgaattcat ttaaagcaat
     3001 gtaagaatca attcaatatt ctttgtagct ttccccacga agaacacgca aagcgcccat
     3061 agccaatgct tccacttcgt tttcgcccgg aaatacaaca attttcccaa atgaggaaac
     3121 catttctttc acgtaattca caaaccattc gcttcgacta atgcctcccg tcagaatgat
     3181 ggcatcaact tttcctttca aaacggcgca catcgctcca atttccttcg caacattgta
     3241 ggccatcgct tccattatca gtttcgcata agcatcgccg ttcaaggctt ttttttcggc
     3301 caaataggca tcgttggttc ccaaatgtgc aatcaggcca ccccttccca ccaacatttt
     3361 ttttatttct tcttttgaaa tttgactttg aaaacaatag tccaccagca atccggcgtc
     3421 gattgttcct gacctttctg gcgaaaacgg gccataacca cccaagccct gattggtgtc
     3481 tatcacttta ccttttgcat gagccgaaac actgattcct ccacccaaat gagcgacaac
     3541 caaattcaaa ttttcgtaac cggttcctgc agaagcagca tattttcgtg ccacagcttt
     3601 ttgattgagc gcgtgaaaca tggaacgccg cggaaattgt ggcaaaccgc tgatacgggc
     3661 aacatcctgc atttcgtcca ccacaacggg atcaactata aaaggcttgc acgacggaat
     3721 atcacgggat atttcataag caatgatccc gcctaaattg cttgcatgtt ctcctctttt
     3781 ggcttctctc agatcggcta tcattctttc attcaccaaa tatacacctg aagcaacagg
     3841 tcgaatcaac ccacctctac caacaactgc agaaagtgtc cgtaaatcga caccatcatg
     3901 attcagactg ttaagaatta gattttttcg gaaatcgagc tgatctataa ttttttcaaa
     3961 ggactttaat tcttcagttg aatgtaaaat ggtatgaaga aataaaattt tttcgtcctc
     4021 ataaacggca aattttgtgg atgtggagcc gggattgatt gaaaggattt taaacatggt
     4081 gtaagattta agtggacttg attcgcaaaa ttagaacaat ttctgaaatc ttttaaaaat
     4141 tcgattcttt tt
//
LOCUS       DAOSCH010000142         4120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_13653_length_4120_cov_6.462731, whole genome shotgun sequence.
ACCESSION   DAOSCH010000142 DAOSCH010000000
VERSION     DAOSCH010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13653_length_4120_cov_6.462731"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..866
                     /locus_tag="PLZ54_08435"
     CDS             315..866
                     /locus_tag="PLZ54_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOJ66985.1"
                     /translation="MMKKLIYLFFKSVSWEIIVPHEIPEKSVICFAPHTSNCDFVIGK
                     LFSWSVGMQFSFLVKKSWFVFPLGYFFKAVGGIPIDRSKSNATVEQMIAEFEKHDRFH
                     LVIAPEGTRSYVPAWKKGFYYIASGANVPIELAFIDYKKKQIGIGKLLYPSGNFSSDF
                     SEIQQFYQNISPRYPEKYNPTIF"
     gene            905..1678
                     /locus_tag="PLZ54_08440"
     CDS             905..1678
                     /locus_tag="PLZ54_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOJ66986.1"
                     /translation="MKITLIQDVIAWSDKQANFNRINRHLETLQGETDLVVLPEMFST
                     GFCTNRLDLAEEGKGETVQTLSNWAKNFGIALTGSFIACENNRYFNRSFFVFPDGNIQ
                     TADKRHLFRHGGEHQYFSAGNRHLLVHYKKWNICVLVCYDIRFPVWSRNVDNRYDLLI
                     YTANFPSKRIRDWDILLQARAIENQAYVCGLNRIGTDGQGIEYNGHSTLVDFKGRFLL
                     TFPENIFDIKTAEISLDSLQNYRQHFPVWMDADRFQIIE"
     gene            1946..2827
                     /locus_tag="PLZ54_08445"
     CDS             1946..2827
                     /locus_tag="PLZ54_08445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ66987.1"
                     /translation="MSIKHLEHSEIDYEKWDKSISQAKNQLVYAETWYLDIVSPEWEA
                     LVADDYKYVMPLPVKTKYAIPYLVQPILTQQLGIFSASEVSEIIIKEFLKRIPYPSYE
                     LNFNEENFIPEAESFPNYVLSLRPTYEELKTKFSKNTKRNIDKAQQAGLQVRNLFSIS
                     DFLDFYYLQEKKFIYPERTLLEILLGQGKEKNAVDIFGVFSPHNELIAALCMIHSKKR
                     LTYWIPVSNAVGKKTSAMFLLVDEIIRQNAERDVILDFEGSRVDGIARFYKGFGSEYK
                     PYFVLKRFRPSFLVGKI"
     gene            2891..>4120
                     /locus_tag="PLZ54_08450"
     CDS             2891..>4120
                     /locus_tag="PLZ54_08450"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOJ66988.1"
                     /translation="MKIHFKIHFITQWGQSIVLLVKNDNHDTPQRIEMNCGKNFEWTA
                     VIDIQPGTKELFYRYALENDSSTLEFEYGDFRQIKIDTEFSDVYISDNWRARYGDSPF
                     ESSAFTDCLFKRNSLYAAPQVKDANLVLRLRCAQLEPNRHFAVIGNQDALGNWDVAKK
                     IRLDESDFPVCSVALNATKFKFPLEYKYLIVDTATDEVLAWGGGPNRVVESVMKKSLN
                     IVNDEHFIRTIPSWKGAGVAIPVFSLRSEESCGIGEFYDLKKMVDWAKKTGQRIIQTL
                     PINDTILYHTNYDSYPYNAVSVYALHPIYLHLESMGTLNDELQKKYFEDKKKEFNAKT
                     FSDYQNVMNLKWEYFEVIYQQEKATVFASESYLNFFVSNKSWLIPYAAFSFLRDFYGT
                     PDFRRWEKYSRYEEQEIL"
BASE COUNT         1301 a          673 c          797 g         1349 t
ORIGIN      
        1 aaaatctaat tgtacatcac ttttttcatt tcatcagaat ataatttatt ttttaagggt
       61 ttgatgaaac ccacatgtct gaatattata ttcattcgtg tttgtgtatg caatacatgt
      121 gggtttcata aaaattttat gctttttgat ctccatctct attaaaaata attaactttg
      181 aaaaactttt tcggaagaaa cttaaacata gaaaaataat ttactgtttt gggaaataaa
      241 aacaacattg aactgaatct ggtcaactga aatattgttt ttatttgctt tttaaaattt
      301 tcctcgagac taaaatgatg aaaaagttaa tttatttgtt ttttaaaagc gtgagttggg
      361 aaataattgt tccccatgaa attcctgaaa aaagcgtaat ctgttttgct ccacacacca
      421 gcaattgtga tttcgttatc ggcaaacttt tctcgtggtc agttgggatg caattttcgt
      481 ttttggtgaa gaaatcatgg tttgtgtttc cgttgggtta tttttttaag gctgtcggag
      541 gtattccgat cgatcgctcc aaaagcaatg ccacagttga gcaaatgata gcagagtttg
      601 aaaaacatga tcgttttcat ttggttatag cgcctgaagg aaccagaagt tacgtgccag
      661 cttggaaaaa aggtttttat tatattgctt ccggtgccaa tgtgcctatt gaattggcct
      721 tcattgatta caaaaaaaaa caaatcggga tagggaaatt gctttatcca tcgggaaatt
      781 tttcatctga cttttctgaa attcagcagt tttatcaaaa tatttctccc cgatatcccg
      841 aaaagtataa tccgactatt ttttagaaaa ttgaatcgga ttgattttta aaatttataa
      901 aaatatgaaa atcacgctta ttcaagatgt tatagcttgg tcagacaaac aagccaattt
      961 taacagaata aaccgacatt tggaaacctt gcaaggggaa accgatttgg tggtattgcc
     1021 cgaaatgttt tcgaccggat tttgtaccaa tcgcctcgat ttggctgaag aagggaaggg
     1081 ggaaacggtt caaacacttt caaattgggc taagaatttt ggtattgccc taacaggaag
     1141 ttttatagcc tgcgaaaata atcgttattt caaccggagt ttttttgttt ttcccgatgg
     1201 caacattcaa actgccgata aacggcattt gtttcgacat ggtggagaac accaatattt
     1261 ttctgccggc aaccgacacc tgcttgttca ttataaaaaa tggaatatct gtgtgttggt
     1321 ttgctacgat atacgttttc ctgtttggtc gagaaatgtg gacaatcgtt acgacctgct
     1381 gatttatact gccaattttc cttccaagcg aattcgcgat tgggatattc tgttgcaagc
     1441 tagagctatt gaaaatcaag cgtatgtgtg tggtttaaac cgaattggaa ccgatggaca
     1501 gggtatagaa tataacggac attctacatt ggtcgatttc aaagggcgtt ttttgctcac
     1561 gtttccagaa aatatttttg atataaaaac tgcagaaata tcactcgatt ctctacaaaa
     1621 ttaccgtcaa cattttccgg tgtggatgga tgccgaccga tttcaaataa ttgaataata
     1681 actttttatg aaacccacat gtattgcata cacaaacacg aatgaatata atattcagac
     1741 atgtgggttc catcagaccc ttaaaaataa attatattct gatgaaaaac aaaatattga
     1801 gtccaattca aaaagtttta tagactttct gcataaaaat tgacttttta aggattgact
     1861 gaatacaaat gatttttatc gtgaggagtg tataaatcat tttttttatg taagtttgtt
     1921 tttacttttg ttaaacaaat tttttatgtc cataaagcat ctcgaacata gcgaaattga
     1981 ttatgaaaag tgggataaat ccatttcgca agcgaaaaat cagttggttt atgccgaaac
     2041 atggtatttg gatattgtat ctccggaatg ggaagcactt gtggcagatg actacaaata
     2101 tgttatgcct ttgccggtaa aaacaaaata tgccattccc tatcttgttc agccgatttt
     2161 gacgcaacaa ctgggtattt tttctgcttc agaagtaagc gaaataatta ttaaagagtt
     2221 tctgaaaaga attccatatc caagttatga attgaatttt aatgaagaaa attttattcc
     2281 cgaagccgag tcttttccga actatgtatt gtcgcttcgg ccaacgtatg aggaactcaa
     2341 aactaaattt tcaaaaaaca ccaaaagaaa tatagacaaa gcgcagcaag ccggtttgca
     2401 ggtgagaaat ttattttcta tcagcgattt tttagatttt tactatttac aggagaaaaa
     2461 atttatttat ccggagcgta ctttgctcga aattcttctc gggcaaggga aagaaaaaaa
     2521 tgctgttgat atttttggcg ttttttctcc gcataacgaa ttgattgctg ctctctgcat
     2581 gattcattcc aaaaaaaggt taacctattg gattccggtt tcgaatgctg ttggcaaaaa
     2641 aacatctgcc atgtttttgc tggtggatga aattattcga caaaatgccg aaagagatgt
     2701 cattctcgat tttgaaggtt cacgagttga tgggatagca agattttaca aaggttttgg
     2761 ttcggaatac aagccttatt ttgttttgaa acgtttcaga ccttcttttt tagtagggaa
     2821 aatttaattt gcttttacta atgacctgtt aacagaataa atacactgat cgacaacaga
     2881 aataaaacac atgaaaattc attttaaaat acatttcatt acgcagtggg ggcaatcgat
     2941 tgttcttttg gtaaagaacg acaatcatga tacaccgcaa agaatagaaa tgaattgcgg
     3001 taaaaatttt gaatggacag ccgttatcga tattcagccg ggcacaaaag aactttttta
     3061 ccgctatgct ttggaaaacg attcatctac attggaattt gaatatggcg attttcgtca
     3121 aataaaaatc gacacggaat tttccgacgt atacatcagc gataattggc gagcacgtta
     3181 cggcgatagt ccgtttgaat cttcggcttt taccgattgc cttttcaaaa gaaattcatt
     3241 gtatgcagcc ccacaggtca aagatgcaaa tctggttctt cgtctgcgat gtgctcagct
     3301 tgaaccaaat cggcattttg ctgttattgg caatcaggat gcattgggga actgggatgt
     3361 tgcaaagaaa attcgtctgg acgagtctga ttttccggtg tgcagcgttg cgttgaatgc
     3421 cacaaaattc aaatttccgc tcgaatataa atatttaata gttgatacgg ctacggatga
     3481 agtgctggca tggggtggcg gaccaaacag ggtggtggaa tcggtgatga agaaatcgct
     3541 aaatatcgtt aatgatgaac attttattcg tacgatacct tcatggaaag gtgccggcgt
     3601 ggccatacct gttttttcgc tgcgcagtga ggaaagttgc ggaataggtg aattttacga
     3661 tttaaaaaaa atggtagatt gggcaaaaaa aaccggtcag cgtatcatac aaactttgcc
     3721 aattaacgat accatacttt atcataccaa ttacgattcg tatccttaca atgctgtttc
     3781 ggtttatgcg ctccatccca tatatcttca tctggaaagt atgggaacct taaatgatga
     3841 attgcagaaa aaatattttg aggataagaa aaaagaattt aatgccaaaa ctttttccga
     3901 ttatcaaaat gtgatgaatc ttaaatggga gtattttgaa gttatttatc aacaggaaaa
     3961 agccacagtc tttgcttccg aatcctattt gaattttttt gtctccaata aatcttggtt
     4021 gattccttat gctgcatttt cttttttacg tgatttttat ggaacgcccg atttcagacg
     4081 atgggagaaa tacagccggt acgaagaaca ggaaatattg
//
LOCUS       DAOSCH010000143         4063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_13943_length_4063_cov_5.684132, whole genome shotgun sequence.
ACCESSION   DAOSCH010000143 DAOSCH010000000
VERSION     DAOSCH010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13943_length_4063_cov_5.684132"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="PLZ54_08455"
     CDS             complement(<1..301)
                     /locus_tag="PLZ54_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOJ66989.1"
                     /translation="MKTTIEQICIQKSSSIRHALKQMDEINRKLLIVLNENNSFFSLL
                     SIGDIQRAIIRNLPLNTRVSEIVRQDITVARSEDNLEKVKERMKIRRNEFMPVIDS"
     gene            complement(305..1225)
                     /locus_tag="PLZ54_08460"
     CDS             complement(305..1225)
                     /locus_tag="PLZ54_08460"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOJ66990.1"
                     /translation="MTVEKNVDLKDIISFLESELMAVYGELDSLVVRHLRDPEHVDEF
                     TLDWINPAKQEKQKVAEASKAKVIIVDKEVVFSETLKNQGKVLLVVDNPKLAISKVGN
                     EFFVEKSTPGIHPSAIIHPDAKIGENVFVGANTVIENCTIGDDTIIDANVHIYSPVII
                     GKHCVVKSGAVLGGMGFGFEKDENGNLFRFPQIGNLFIGDYVEIGANTCIDRGALSDT
                     IIGDYCKINNLCHIAHNNKIGKNVVITAQVNISGSNVIEDDVWIAPNATIRGWITIGK
                     GATIGMGSVVTKDVPAGETWIGNPARKLEK"
     gene            complement(1233..2552)
                     /locus_tag="PLZ54_08465"
     CDS             complement(1233..2552)
                     /locus_tag="PLZ54_08465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ66991.1"
                     /translation="MPKKKMKVAWICHFSNEKVREKLPLSKRIFLNRLKKLLGMQPYV
                     YSDFAPWVNNLITEFEKFEDVELHIIAPHAGLSPFKYSFEMNGIYYHFFRPELPLRLD
                     ILVSRLPHQEKRRFLLNRLWVRQFIKEIQPDVVNLIGTENPYYSITVLDIKDIPVYVS
                     AQTVYTNPDRKKLSGVCDRLHWNTELKIHHKEKYYGCSGRMHRDLILRNNPDAMIFKN
                     FFPIQHPGNIKEVEKEFDFVYYAQHVSAKKGIEDAIDALSIVKKEKPDVTFKVVGNCP
                     PNYRDFLNEKISSLGLTENISFHDYFPVHSEMHQYIKKSRFALLPIKLDVISSSVLEA
                     MLLELPLVTYKTTGTPYLNKDGETVLISEIGDIEHLAANMLKLLNSPELAERLKKKAK
                     AFVEKEFDNTASAKRLVEDYKAVIAHYHNGTPILKELLFDLNEFPIY"
     gene            complement(2503..3813)
                     /locus_tag="PLZ54_08470"
     CDS             complement(2503..3813)
                     /locus_tag="PLZ54_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66992.1"
                     /translation="MNKYIELLKSNFSRIKNFSLYLSAAIVSGVLSILFNPFLAANLS
                     SLDYSIIGYYSSFVPLFTPILNFSLISFYVRKYFKIPEERRQLTLDTIITALLVWGVV
                     SSLLILAGFFIYCRLNKVELPFFPFALMSVCQLFFNNFLLLYQVNCRMRSEAKRYFKI
                     TMTASLLAIFFSIPLVIVFKFGAVGRMTALLISSFVVGLFSLKKTLSKLRCDWGILKE
                     AVSFGWPVSTTYIIQYFLVGIDLALLEPLNDIHSMGLYAIAISISGYLSLFYTALAQT
                     FEPDTYKAVAQYDYRKIGKIVMLIVSILTAVVLVFVVLAKPVIAILTANRYTDAYHFA
                     RILSIGVVSSYLMVAIEGIINAFGFTKICLTNKIISSAIAVFLYIYLINLYGFYGAAW
                     GRVIAPLLVFIAGCVSLILLKNRIKTMRYAEKKDESCLDLPFLE"
     gene            complement(3853..>4063)
                     /locus_tag="PLZ54_08475"
     CDS             complement(3853..>4063)
                     /locus_tag="PLZ54_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66993.1"
                     /translation="PCASFIPNVYVDITDYLDQKIEIMKLYETELMEEPYPRSISSIE
                     ALARFRGSRAGVKYAEAFMLLYEKI"
BASE COUNT         1320 a          798 c          674 g         1271 t
ORIGIN      
        1 ggctgtcgat gacaggcata aattcatttc tgcggatttt catgcgttct ttcacctttt
       61 caaggttgtc ttccgaacgg gcaaccgtga tatcctgtct gacaatctcc gacacacggg
      121 tattcaaagg caaattccgg ataattgccc gttgaatatc accaatactc aataagctaa
      181 aaaagctgtt gttctcattt aaaacaatga gcaatttccg gtttatctcg tccatttgct
      241 ttaaagcatg acggatagaa ctgctttttt gtatacaaat ttgctcgata gttgttttca
      301 tgcgttattt ttctaatttc cgagccggat tgcctatcca tgtttcgccg gcaggaacat
      361 ctttggtaac aacggacccc ataccgatag ttgcgccttt cccgatggtt atccaacctc
      421 gaatggttgc attaggcgct atccagacat catcttctat cacgtttgat cctgaaatat
      481 tgacttgtgc cgtaattacg acattcttcc cgatcttgtt attatgcgct atatggcaca
      541 gattattgat tttacaataa tctccaataa tggtatcaga taaagcacct cggtctatac
      601 atgtatttgc tcctatttcc acataatccc cgataaataa attgccaatt tgaggaaaac
      661 gaaacagatt accgttttca tccttttcaa agccgaagcc cattcctccc aacacagctc
      721 cagatttcac cacacaatgt ttgccgataa ttaccggaga ataaatatga acatttgcat
      781 caattatcgt atcatcacca atagtacaat tttctattac agtattggct ccaacaaaaa
      841 cattttctcc aattttggca tcgggatgaa taatggcgga aggatgtatg ccgggagttg
      901 atttttccac aaaaaattca tttcctactt ttgaaatggc taattttgga ttgtctacca
      961 ccaataaaac ttttccttga tttttcaagg tttcggagaa tactacttct ttatcgacaa
     1021 tgatcacttt tgcttttgat gcttctgcta ctttttgttt ttcctgtttg gccggattga
     1081 tccaatccaa tgtaaattca tcgacatgtt ccggatctct cagatgcctt acgacaagag
     1141 agtcgagttc cccgtaaaca gccatcagtt cgctctcaag aaacgaaata atatctttta
     1201 aatcaacatt tttctccacg gtcataaatc ttttaataga ttgggaattc atttaaatcg
     1261 aaaagcagtt cctttagaat cggtgtccca ttgtgataat gtgctatcac tgccttgtaa
     1321 tcttccacga gtctttttgc agacgcggta ttgtcaaatt ccttttcaac aaaagctttt
     1381 gctttctttt ttaatcgttc ggccaactcc ggagaattta gcagttttag catatttgca
     1441 gccaagtgtt caatatctcc aatttcagat atcaacacgg tttctccatc tttattcaaa
     1501 taaggtgtac cggtagtttt ataggtaacc agaggtaatt ccaacaacat ggcttcaagt
     1561 acgctgctgg agataacatc aagcttaatg ggcaatagag caaatcttga tttttttatg
     1621 tattgatgca tttcggaatg tacaggaaaa taatcgtgaa aagaaatatt ttccgttaat
     1681 cccaaactgc tgattttttc atttaagaaa tctcgataat tcggaggaca attacccact
     1741 actttgaatg taacgtccgg tttttctttt tttactatag ataaagcgtc aatggcatct
     1801 tcaattcctt ttttagcaga aacatgctgc gcataatata caaaatcgaa ttctttttcc
     1861 acttccttta tattccccgg gtgctgaatt ggaaagaaat ttttgaatat cattgcatca
     1921 gggttgttac gtaatattaa atcgcgatgc attctacctg aacaaccgta atatttttcc
     1981 ttgtgatgaa ttttcaactc tgtattccaa tgaagtcgat cacaaacacc gctaagtttt
     2041 tttctatcag gatttgtata aacggtttga gccgaaacat aaacgggtat gtctttaata
     2101 tccaataccg ttatggaata gtacggattt tctgtaccaa tcaaattgac cacatcaggc
     2161 tgaatttcct taataaattg tctgacccac aacctattca gtaaaaatcg tcttttctct
     2221 tgatgaggta gtctgcttac aagtatatcc aaacgtaatg gcaactcggg ccgaaagaaa
     2281 tgataataaa tgccattcat ttcaaagctg tatttgaatg gtgataaacc tgcatgagga
     2341 gcaataatat gcaattccac atcttcaaat ttttcaaatt cagtaatgag attattaacc
     2401 caaggagcaa aatcggaata cacataaggt tgcattccaa gtaacttttt taatctattc
     2461 aaaaaaattc ttttggaaag gggcaatttt tctctgactt tttcattcga gaaatggcaa
     2521 atccaagcaa ctttcatctt ttttttcggc ataacgcata gttttgattc tattttttag
     2581 caatatcaat gacacacaac ctgcaataaa aaccaataat ggggcaataa cccgtcccca
     2641 ggcagctcca taaaagccgt acaaatttat caaataaata taaagaaaaa cagctattgc
     2701 agaactgatg attttatttg tcaaacaaat ttttgtaaat ccaaatgcat tgattattcc
     2761 ttcaatggca accattaaat aagaagaaac aaccccgatg gataatattc tggcaaaatg
     2821 gtatgcatcg gtatatcggt ttgctgtcag aatagcaata acgggtttag ccaatactac
     2881 aaataccagg acaacagcag tgagaataga aacaataagc atcacgattt taccaatttt
     2941 tctgtaatca tactgtgcca cagccttata agtatccggc tcaaaagttt gagccagtgc
     3001 agtgtaaaat aaggatagat aaccggatat agatatggct atggcataca atcccatact
     3061 atggatatca tttagcggtt ccaataatgc aagatcaatg cccactaaaa aatattgaat
     3121 aatgtaagtc gttgaaacgg gccaaccaaa ggaaactgcc tctttgagga ttccccaatc
     3181 gcaccttagt ttcgaaagtg ttttttttaa actaaaaaga ccaacaacaa atgaagaaat
     3241 cagcaaggca gtcattcgtc cgacagctcc aaatttgaaa acgataacca atgggatgga
     3301 aaaaaatatc gccagcagtg aggcagtcat ggtaatttta aaataacgct tggcttcgct
     3361 gcgcatccgg caattgacct gatagagaag tagaaaatta ttaaagaaaa gttgacatac
     3421 gctcatcaaa gcaaagggga agaaaggcaa ttccacctta ttaagtcgac aataaataaa
     3481 aaacccggcc aaaatgagca acgaagaaac cacaccccaa acaagcaacg ccgtaattat
     3541 ggtatccaat gttaactgtc ttctttcttc aggtatttta aaatatttcc gaacataaaa
     3601 agaaatgagt gaaaaattca atattggagt aaaaagtggt acaaaagaag aataataacc
     3661 aataatggaa taatccaacg aagaaagatt ggcagccagg aacggattga ataatatact
     3721 taaaacaccc gaaacaatag ccgctgataa gtataaagaa aaattcttaa tcctggaaaa
     3781 attgcttttt aaaagttcga tgtatttatt cattgaattt atttatatgg tatgtaaaga
     3841 gaatattttg tattatatct tctcatacaa caacataaat gcttctgcat attttactcc
     3901 ggctctgctg ccacgaaaac gtgccaaagc ttctattgaa ctaatacttc gcgggtaggg
     3961 ctcttccata agttctgttt catatagttt cattatttca atcttttggt ctaaatagtc
     4021 ggttatatct acataaacat taggtataaa tgatgcacat ggc
//
LOCUS       DAOSCH010000144         3969 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_14361_length_3969_cov_6.741799, whole genome shotgun sequence.
ACCESSION   DAOSCH010000144 DAOSCH010000000
VERSION     DAOSCH010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14361_length_3969_cov_6.741799"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            164..376
                     /locus_tag="PLZ54_08480"
     CDS             164..376
                     /locus_tag="PLZ54_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004310589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ66994.1"
                     /translation="MVKSNKNKLAKIVKRLREQRGLSQEKLARLADVSNNTIINIEAG
                     KQDNPTIETLKKVAKALNVPVENLIK"
     gene            383..1381
                     /gene="dcm"
                     /locus_tag="PLZ54_08485"
     CDS             383..1381
                     /gene="dcm"
                     /locus_tag="PLZ54_08485"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HOJ66995.1"
                     /translation="MANNKSNNLTFIDFCAGIGGGRIGLENLGMKCVGFSEIAPSYIR
                     TYRMFFGEDEPNYGDLMKIDPKNLPDFDLMIAGFPCQPFSVIGPRTGMYDRRGQIIFG
                     LIDIMTGKNLKYFILENVKGLLNHGDGRTLKIILDELEKAGYKVFWKLVNSVDYGVPQ
                     MRERIYFVGIRKDLVKDGLPFEFPKHAPRPPLKDYLIDTAELEFDEKQRAYETFLKYL
                     NNKYNKGKFSIEKLLSEEYLVIDTRQSDLRLYRGMVPTLRTGRHGILYVRNGKFRRLS
                     GYESLLLQGWPKELANKVRGKVDEVVLLSQAGNAMTTHVVEAFGRSLLDFINKHER"
     gene            1371..2123
                     /locus_tag="PLZ54_08490"
     CDS             1371..2123
                     /locus_tag="PLZ54_08490"
                     /inference="COORDINATES: protein motif:HMM:NF022902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ66996.1"
                     /translation="MSDKDLVKLGSVTAKGGFRNEDDIVAKFNNWKNDEDAQKWLEIM
                     GYPINEIEKVEAVKLHGYKTDVQVQITIYMKKAIAAENLSVKLVSNPQGFNQVDKRWV
                     DSYADMWNIPKDIVKILKLFTGEIRPTKSGLRDPRRMFLDEMNENDQRKVVDFFAKNK
                     ILIVSDILKGRGKFSAGWMLVVLIINNESRWVLKSINHAMNVFADGEVRITPQGSLKI
                     GKIGMQRKGGDAGRPTSNMLQFKINPVELFKD"
     gene            2165..2521
                     /locus_tag="PLZ54_08495"
     CDS             2165..2521
                     /locus_tag="PLZ54_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HOJ66997.1"
                     /translation="MSKVKSKDSKIEVDFRKAMWKAGFRYRKNSAKYFGKPDMVLPKH
                     KTAIFIDSCFWHGCKRHCRLPAARKKYWTTKIERNKERDKEVNRHYKKIGWKVIRVWE
                     HEIKKKTDAVIRKIKI"
     gene            2518..>3969
                     /locus_tag="PLZ54_08500"
     CDS             2518..>3969
                     /locus_tag="PLZ54_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOJ66998.1"
                     /translation="MKIKELTISNFRGVKNLTLNSKELSIFIGDNTTGKTSIIEAINY
                     ALSPHFLTGRIKYTDFYRGTDDPIVIKVEFDEIFTALLPDGYAVQKIECNGVYLEIKK
                     RDKATPGKAFSDMVVLTHYVIPNRPKDSEQGWEITRKSGTKFQFDERLLSLSRVDLEE
                     FPKSFYFGKNREKQLRQGFNSSISAVFDDFNWRFSKGIRSDGNENFFTKKNAFEKEII
                     SKVDETSIKKSFETLNDKLKNFGVCGIDLSFIDGNAPFDSAFLSQKLEKLDLPVAALG
                     SGIEMIVALLFLETLASLSNEKIIVLIDEPELHLHPQLQEKLVQYLVEFSKTSQVFIS
                     THSPYFFKNCLQNSQIELLITKNSADGVVIENTSSQFGLFPWSPSWGEINYSAYNLPT
                     IEFHNELYGYIQEKQQKYTIADIEAYFVSKGIRKSKKWAKIVNGRIQPQEDVTLMTFI
                     RNVIHHPENTNNGSFTLQELRTSTEELIKLIKNP"
BASE COUNT         1388 a          637 c          869 g         1075 t
ORIGIN      
        1 ctatttatat ttagtcgtcc ttgtcccgcc ccgcccaacg cgggcgacac gaaggaactc
       61 cctttaatct ttacttttgg aaaatatttt gttaatatta aattatggga agcaatcttg
      121 ttcacaactt caatatacca caatttaata ttaccaatca attatggtaa agtcaaataa
      181 aaacaaattg gcgaaaatcg taaaacggtt aagagaacag cggggtttat cgcaagagaa
      241 gttggcccgt cttgccgatg tttcaaacaa tacaataatc aacatagagg cgggcaaaca
      301 ggataatcca accattgaaa cgcttaagaa agtggcaaaa gcgttaaatg tacccgttga
      361 aaatttaatc aagtgaagat ttatggcaaa caataaaagc aacaatctta catttattga
      421 tttttgtgcc ggcattggag gcggccgaat cggtcttgaa aatttgggaa tgaagtgcgt
      481 cgggttttcg gaaatcgccc cgtcgtatat aaggacttac agaatgtttt ttggcgagga
      541 tgaaccgaat tacggagatt taatgaaaat tgaccccaaa aatttgccgg attttgattt
      601 aatgatcgcc ggatttcctt gtcagccttt ttccgtcatt ggaccgagaa cgggcatgta
      661 cgaccgaaga gggcaaatta ttttcgggtt gatagacata atgacgggaa aaaatttaaa
      721 gtactttatt ttagaaaacg tcaaaggttt gttgaaccat ggagacggac ggaccttgaa
      781 aattattctc gacgagttag agaaggcggg atataaagtt ttttggaaat tggttaatag
      841 cgtagattac ggagtgccgc aaatgaggga aaggatttac tttgtcggca tccgtaagga
      901 tttggtcaaa gacggccttc cgtttgaatt tccaaaacac gcgccaaggc cgcctctaaa
      961 ggattatttg atagatacgg cggaattgga attcgacgaa aaacaaagag cttacgaaac
     1021 ctttttgaag tatctaaaca ataaatacaa caaagggaaa ttttccattg aaaaattgct
     1081 gagcgaggaa tatttggtta tagatacccg acaatctgat ttgcggttat atcgcggcat
     1141 ggttccaact ttgagaacgg ggcgacatgg tattttgtac gtgcggaacg gaaaattcag
     1201 aagattgtcg ggttacgaat ccttgctttt gcaaggatgg cccaaagaat tggcgaataa
     1261 agtcaggggc aaggttgatg aagtcgttct gttaagtcaa gccggaaacg ccatgacgac
     1321 gcacgtcgtc gaagcgttcg gcagaagtct tttagatttt ataaacaaac atgagcgata
     1381 aagatttagt caaactagga tcggtcaccg ccaaaggtgg atttagaaat gaggacgaca
     1441 tagtcgccaa gtttaacaat tggaaaaatg acgaagacgc gcaaaagtgg ttggaaatta
     1501 tgggttatcc aattaacgaa atagagaaag tggaagccgt aaaattgcac ggatataaaa
     1561 cggatgttca agttcagata acaatttata tgaagaaagc gatcgcggcg gaaaatttat
     1621 cagtgaagtt agtcagtaat ccgcaaggat ttaatcaggt tgacaaacga tgggtggata
     1681 gttatgcgga tatgtggaac ataccgaaag atatcgtgaa aatcttaaaa ctttttactg
     1741 gagaaataag gccgacaaaa tccggcttgc gggatccccg aagaatgttt ttggacgaaa
     1801 tgaacgaaaa cgaccaaaga aaagttgtgg actttttcgc caaaaataaa attttgattg
     1861 tctccgatat ccttaagggc agaggaaaat tttcagccgg ttggatgttg gttgttttaa
     1921 taatcaataa cgaaagtcgc tgggttttga agtcaatcaa tcacgcgatg aatgttttcg
     1981 cggacggcga agtgaggata acaccgcaag gcagtttgaa aatcggaaag ataggcatgc
     2041 aaagaaaagg cggcgacgcc ggacgtccga cttctaacat gcttcaattc aaaatcaatc
     2101 cggtggaact tttcaaagat tgactatgac ggacactgtt tcaaaaaaga aaagaagcga
     2161 gataatgtct aaagtgaaaa gcaaagacag caagattgaa gttgatttca gaaaagcgat
     2221 gtggaaagcg ggatttagat ataggaaaaa ttccgcaaag tatttcggca agcctgacat
     2281 ggttttgcca aaacacaaaa cggcgatttt cattgattca tgtttttggc acggatgcaa
     2341 acgccattgc cgactaccag cagcgaggaa aaagtattgg acgacaaaaa tagaaaggaa
     2401 caaagaacga gacaaagaag ttaatcgaca ttacaaaaaa atcggttgga aagttatcag
     2461 agtttgggaa catgagatta aaaaaaagac cgatgcagtt atcagaaaaa taaaaatatg
     2521 aaaattaaag aactgacaat cagtaatttt cgtggggtaa aaaatctaac actcaattca
     2581 aaagagcttt caatttttat tggggataac acaactggta agacttccat aattgaagca
     2641 ataaattatg ctctctcgcc acatttttta actggaagga taaagtatac agacttctat
     2701 cgaggaacag atgatccaat tgtcattaag gttgaatttg atgaaatttt taccgctttg
     2761 cttcctgacg gttatgcggt gcaaaaaata gagtgtaatg gtgtttattt ggaaatcaag
     2821 aagagagaca aagcaacacc agggaaggct ttttcagaca tggttgtatt aacacattat
     2881 gtaataccaa atagaccaaa agacagcgag cagggatggg aaataacacg aaagagcggg
     2941 acgaaatttc aatttgatga aagattgctt tctctatctc gtgtggattt agaagaattt
     3001 ccaaagagtt tttattttgg caaaaacaga gagaaacagc tgcggcaagg ttttaattct
     3061 tctatttctg ccgtctttga cgattttaat tggcgttttt ctaaaggcat taggtcagac
     3121 ggtaatgaaa atttttttac gaaaaaaaat gctttcgaga aagaaataat aagcaaagtt
     3181 gacgagactt cgattaaaaa aagttttgaa acattgaatg acaaattaaa gaattttggt
     3241 gtctgcggta ttgatctttc gtttattgac ggcaatgcac catttgatag tgctttttta
     3301 tcacaaaaac tcgagaaact cgatttacca gtagcggctc tcggttctgg tatagaaatg
     3361 atagtggctt tattattttt ggaaactcta gcctcgttat caaatgaaaa aattattgtt
     3421 ttgatagacg agccagaatt gcatttgcac cctcaattac aggaaaaact ggttcaatac
     3481 ctggttgaat tttcgaaaac aagccaagtt tttatttcta ctcattcgcc gtattttttt
     3541 aagaattgtc tgcaaaattc tcagatcgaa ttgttaatta caaaaaattc tgcagatgga
     3601 gttgtgatcg aaaacactag ttcacagttc ggtctatttc cgtggagccc gtcttgggga
     3661 gaaataaatt attccgcata taacttacct acgatagaat ttcacaatga actttacgga
     3721 tacatacaag aaaaacaaca aaaatacaca atagctgaca ttgaagcata ttttgttagc
     3781 aaggggataa ggaaatctaa aaaatgggcc aaaattgtga atggtcgcat tcaaccacaa
     3841 gaagatgtaa cattaatgac ttttatcaga aatgttatac atcacccaga gaatacgaat
     3901 aatggcagtt ttacattaca agaattgagg acgtcaacag aagagttaat caagttgatt
     3961 aagaatcca
//
LOCUS       DAOSCH010000145         3703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_15821_length_3703_cov_7.781524, whole genome shotgun sequence.
ACCESSION   DAOSCH010000145 DAOSCH010000000
VERSION     DAOSCH010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15821_length_3703_cov_7.781524"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            604..1242
                     /locus_tag="PLZ54_08505"
     CDS             604..1242
                     /locus_tag="PLZ54_08505"
                     /inference="COORDINATES: protein motif:HMM:NF039392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6266 family protein"
                     /protein_id="HOJ66999.1"
                     /translation="MGTIKQGILGGFSGKVGTVIGGNWKGISYMRSQAQHIKNPRTDA
                     QVSQRAKFALALNFLKPMTHVLRVGFKLYANKQTAFNAAMSYTLANAITGEYPDYELN
                     FASALISRGTLTSAANGAVAVADGKVTVSWEDNTGVGTAKASDKALIALANLIKGEAI
                     TEDGGATRSAGSQSIDIPSDWIGDEVQVYLGFVSEDAKEVANSVYLGSVTIA"
     gene            1341..1796
                     /locus_tag="PLZ54_08510"
     CDS             1341..1796
                     /locus_tag="PLZ54_08510"
                     /inference="COORDINATES: protein motif:HMM:NF016961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOJ67000.1"
                     /translation="MKPLFIPYLINHDWNHLKTVLTVVAVLYVCVFLSVLIDLFFGVR
                     RAKRLNIVRTSSNYRRTITKLTNYFGLMLMLSIADIVASVVFKMPYFTVTGAIGIVLV
                     EAKSVFENLKDQEKSMEEVQKMLLKILENKEEIKTILSLLNTHKEENKL"
     gene            1793..2203
                     /locus_tag="PLZ54_08515"
     CDS             1793..2203
                     /locus_tag="PLZ54_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="structural protein P5"
                     /protein_id="HOJ67001.1"
                     /translation="MTPRGLRNNNPLNIRHSADHFQGEIAGTDKIFKTFSSLAYGYRA
                     AFVILANYNLRGWNTIEKIISHWAPPAENDTENYISQVEKYSGVPRNKELTLANGTEY
                     MMIVAAMSFVENGQNADISQVQAGFNLQTKIKMK"
     gene            2200..2595
                     /locus_tag="PLZ54_08520"
     CDS             2200..2595
                     /locus_tag="PLZ54_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67002.1"
                     /translation="MRKIILSTVIATFIFACTSCKTTKYIPIETTKIEYRDNLLRDSI
                     IHYDSVYVKDKGDTLILEHYKYLYKNKMIKDSIYLNDTIRIPFPVEVVKPVKASLSSW
                     QNFQLWCGRIALVLVVLVGLYLVIKSKKF"
     gene            2840..3604
                     /locus_tag="PLZ54_08525"
     CDS             2840..3604
                     /locus_tag="PLZ54_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67003.1"
                     /translation="MKTFKIISVILIISAFLLSCKNDQIIEERTKLLPSEIIGLTEDT
                     KEIITYNEDNMIIGYKSVYNDYDESEIYTIEYENKKPSRFTENQYGEIIEYNITYSND
                     GTHVYLTDTRNVSENIAPNYNLTIDKSGNLLVLFVDEPAGFQDYQINYEYDNLNNWTF
                     YSQNGRTFEYDTTKKGIFVNSATPNWVSTYYSWFVEGIFSPNLLVKSVGLTDNSRVNI
                     YEWSEYNDDGYPEKLTIKLSDSDEPLEYKINYTEAK"
BASE COUNT         1204 a          712 c          644 g         1143 t
ORIGIN      
        1 agccaacgaa cgtccatttt tcgtgcgaag cgggtagagt cttgaccttt tttgcttcgt
       61 tttttgtggc aaaacaaaaa atgaagtggg gtttggggca acgccccata tttttacctt
      121 tttgttcggc gtagcctcca gactacgtcg taagtaaaaa taaaatctcc agattttaat
      181 attgccattc ctcaaatttt atcgtaaaac tttctaaaag atagcaaccc aactttcgta
      241 gttgaaagaa aaaataaaat gaacgcaaag ataaggcggc gttcccttat atcctcccac
      301 tttgtccgtc aaggttaagc cctgtgggtt ttgcttaaaa tctccacacc ttcgggtagt
      361 atttataagc aaaacccttg acagacacca acgccgccat tcttatatag ctatcatttt
      421 attttttcca cctttctttt ttatccaaca gcaagagaac gcaaaccaac gcaaaagaaa
      481 cttcttccgc accaacctca attttatgca gtaaattagc ttatgaataa ggtttgagac
      541 gtattttcct cataccaact ccaagtaatt ttctattcat tcacccttta aatttttact
      601 aaaatgggaa ctatcaaaca aggtattttg ggcggctttt ccggtaaggt cgggacagtc
      661 attggtggca attggaaagg gatttcctac atgcgaagtc aagcccaaca cattaagaat
      721 cctcgtactg atgcacaggt cagtcaaagg gcaaaatttg ctcttgcctt gaatttctta
      781 aaacctatga cccatgtgct acgggtagga tttaaactct atgccaacaa gcagactgct
      841 ttcaatgcag ctatgtctta cacgttagca aatgccatta cgggagagta tcccgattat
      901 gagctgaatt ttgcttctgc tctcatttct cgtggtacgt tgacttccgc tgccaacggt
      961 gcagtggctg tagctgacgg aaaagtaacc gtttcctggg aagacaacac aggcgtcggc
     1021 acagccaaag catctgacaa ggctctgata gcactcgcta atctcatcaa aggggaagca
     1081 atcaccgaag atggtggagc tactcgttcc gcaggctctc aaagcattga cattccttcc
     1141 gattggatag gcgacgaagt gcaggtttat ctgggtttcg tttccgagga tgccaaagaa
     1201 gttgccaaca gcgtctatct gggttctgta accattgcct gaacgtaggc agttttctgt
     1261 tttcttgctg tcaaatcccg ctctctgtgg cgggattttt taaagcaatt acatgtaatc
     1321 aactttaaaa tctattacaa gtgaaacctc tttttattcc ttatctcatc aatcacgact
     1381 ggaatcattt aaaaactgtc ctcaccgttg tggctgttct ttatgtctgt gtatttcttt
     1441 ctgtactgat tgatctgttt tttggagtac gacgggcaaa acgattgaat atcgtccgta
     1501 caagttccaa ttatcgtcgt accattacca aacttaccaa ttattttggt ttaatgctca
     1561 tgctttctat tgctgatatt gtagccagtg tagtttttaa aatgccctat tttactgtta
     1621 ccggagcgat aggtattgta ctggtagaag caaaatctgt ttttgagaac ctgaaagatc
     1681 aggaaaaaag catggaggaa gttcaaaaaa tgttattaaa aatactggaa aacaaagagg
     1741 aaatcaaaac aattctttct ttgttaaata cccataaaga agagaacaaa ctatgacccc
     1801 gagaggactt cgcaacaaca atccattgaa tatccgccac agtgctgatc attttcaggg
     1861 tgaaattgcc ggaacggaca aaattttcaa aaccttttct tctcttgctt atggctatcg
     1921 tgctgccttc gtaatccttg caaactataa tctgcgaggt tggaatacca ttgaaaaaat
     1981 tatatcccat tgggcaccac cagctgaaaa cgatactgaa aactacattt cccaggttga
     2041 aaagtattcg ggtgttcccc gaaacaaaga acttaccctt gccaatggaa ctgaatacat
     2101 gatgatagtg gcagccatga gtttcgtcga aaacggacaa aatgctgata tttcgcaagt
     2161 gcaggcaggt ttcaatcttc aaaccaaaat caaaatgaaa tgagaaaaat tattttatct
     2221 accgttattg caacattcat atttgcttgc acttcctgta aaactaccaa gtacattccc
     2281 atagaaacta caaaaatcga gtatcgggac aatcttctca gagattcaat tattcattat
     2341 gattctgtct atgtaaaaga taaaggcgac acactgattt tggaacatta caaatatctt
     2401 tacaaaaaca aaatgatcaa agattcaatc tacctaaacg acactatccg cattcccttt
     2461 ccggtggaag tcgttaaacc agtcaaagcc tccctttcaa gctggcaaaa ctttcaactc
     2521 tggtgcggac gaatagccct tgtccttgtc gtgcttgtag gcctttatct tgttattaaa
     2581 tcaaagaaat tctaatttct aaaccagaca ataatatttt gaaactattt tgttttttta
     2641 ttgtccttat cattacttat tgaaaaatca ttcatatttt tgtaaatcaa attacagatt
     2701 ttcagttttt gtttatttat gatagtgcaa acaatgtatc atatttgtac cactttatct
     2761 atattttatt actattcaat taattatact ttttgtatcg gaaaatagct ctttctaaca
     2821 ataataaaaa cacataaaaa tgaaaacatt caaaatcatt tcggtaattt taataatttc
     2881 tgcatttctg ctttcgtgta aaaatgatca aattatagaa gaaaggacaa agttgctgcc
     2941 ttcggaaatc attgggctaa cagaggacac caaagaaatc attacctata acgaagacaa
     3001 tatgataatc ggttataaat cggtttacaa tgattatgat gagagtgaga tttatacaat
     3061 agagtatgag aataaaaaac caagtcgatt tacagaaaac caatatggcg aaataatcga
     3121 atataatatt acctattcaa atgatggtac gcatgtttat ttgacagata cacgaaatgt
     3181 aagtgaaaat attgctccaa attataattt aaccattgat aaatcgggga atttgttagt
     3241 gctgtttgtg gatgaacctg ccggttttca agattatcaa atcaactatg agtatgataa
     3301 tttaaataat tggacatttt attctcaaaa cggacgtact tttgaatatg atacaacaaa
     3361 aaaaggaatt tttgtcaatt cggcaacacc taattgggta tcaacctatt atagttggtt
     3421 tgttgaaggc atattttctc cgaacttact tgtaaaaagt gtgggattaa ccgacaattc
     3481 gagagtaaat atctatgaat ggtcggaata taacgatgac ggttatcccg aaaaattgac
     3541 aatcaaatta agtgattctg atgaacctct tgaatacaaa ataaactaca cagaggcaaa
     3601 ataatttttt ttgaaatatt aaaagaatgg aacactgtac tacgttcaca taccgacaat
     3661 ttaggctcca ttcaagccat aacagacgaa aataaaaaca tcg
//
LOCUS       DAOSCH010000146         3674 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_15984_length_3674_cov_4.831445, whole genome shotgun sequence.
ACCESSION   DAOSCH010000146 DAOSCH010000000
VERSION     DAOSCH010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15984_length_3674_cov_4.831445"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="PLZ54_08530"
     CDS             complement(<1..570)
                     /locus_tag="PLZ54_08530"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOJ67004.1"
                     /translation="MKMNNFFKYFSFYALIFGFFLFSSLNLWASNEKKINVKIACVAF
                     YNQENLFDTIDNENVNDYEFTPNGPNRWNSMKYKSKLEKMSYAISQIGLDVTPDGAAI
                     IGVSEIENRLVLEDLVNQPALKKRSYEIVHYDGPDRRGVDVALLYNPKYFIVTNSKSC
                     RLHTADTTFRTRDQLMVSGYLFGEKIHVIV"
     gene            961..>3674
                     /locus_tag="PLZ54_08535"
     CDS             961..>3674
                     /locus_tag="PLZ54_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOJ67005.1"
                     /translation="MKKLFLILFVFFLCIPLLMAETSIKGTVKSADTQNPIPGVIVSL
                     IGQNISTQTNAEGQFSLRFIEAGDQEIRISKIGYFTQIKLIHIEPDKENDLGTFYLRE
                     DVQQQTKQEVVLQLSENQIDEDEGRATQNISAALSSKGDVYVSQSSYSFSPMRFRTRG
                     YDDNFESTYINGVYFNSLERGGFNYSSLGGLNDAMRNQDYTYGFAPNSFSYGNLGGTT
                     NINTKASNYAAGTKASVAYTNRSYKLRGQATYATGLMQNGWAFAGSAVVRWADKGIVD
                     GTFYNSWGYFFSAEKILNKQHSLSFVTFGSPTQRAQQGAVTQEVFDLAGSIYYNPYWG
                     YQEGKIRNSRIVKSFDPTAILSHEYKISNHQLLRTGLAFHYSLYSNSALNFYNAPDPR
                     PDYYRNLPSFVSDDSLKNVIANQWKSDTNVSQVNWGALYQANYKNNVINPNGTARYIV
                     ERRLNNLMEGALNSTYTQQVNKQLKLIAGIEGKMSKGMHYKTLDDLLGGNQWIDIDQF
                     SERDFPSNPDIIQNDLRNRNAVIKEGDTFGYNYDINMAYANGFIQNEWILPQFDIYYA
                     AKLTYTQFYRYGHMQNGRAPENSYGKSKTWYFVDPSFKAGFTYKIDGRNRLSFNGLIE
                     SRAPLASQAYLSERIKDMLIPYLQSQDIRSADINYNFTYSTIRGRIGVFQTNLYNTSD
                     MLGYYDDEYRTFINHVLAKSDKVYKGVELGTTVKINSNFSVSLAGTYADYHYANNAIG
                     VKSPENGAFADVAETVLTKGLKIAAGPQAAGNITIDYFHPKMWFADVTLNYFDNNYLD
                     FAPNRFTENNMAKYTTPEMMAALGTQEKLKGGFLLDASVGKLIYLKHRRSLNFNLSAS
                     NILNNTKMITGGYQQARLPLSNGAIDPTGLNRFPNKYYYAWGFNVFFN"
BASE COUNT         1201 a          728 c          672 g         1073 t
ORIGIN      
        1 gacaatgaca tggatttttt ctccaaaaag ataaccgctt accattagct ggtcacgcgt
       61 acgaaaagtg gtatcggcgg tatgcaatcg gcacgatttg ctgttggtaa caataaagta
      121 tttaggatta tacaatagag ccacatctac acctcgacgg tcggggccat cgtaatgcac
      181 aatttcatac gaacgttttt ttaatgccgg ctgattcacc aaatcttcca acaccagtcg
      241 attttcaatt tcagaaacac caatgatggc agctccgtca ggtgttacat ccaacccgat
      301 ttgagaaatg gcatacgaca ttttctcgag cttcgatttg tatttcatcg aattccaacg
      361 atttgggcca ttgggtgtaa attcataatc gttaacattt tcattgtcaa tggtatcaaa
      421 taagttttcc tggttgtaga aagctacaca ggcaattttt acgttgattt ttttctcgtt
      481 cgacgcccaa agattgagtg atgaaaacag aaaaaagcca aaaattaaag cgtaaaaaga
      541 aaaatactta aagaaattgt tcatcttcat ggcaaaaaaa attattgaga atttatcgtt
      601 ttattaaaga gggacaaatg tagtaaaaaa gaaatacaaa gaaaaaattt gaaaaataaa
      661 tttagaattt tgtcaacaaa tctcacaaat tactgtttta attaaaatta aaaatttcat
      721 ttacaactaa ttaatcatat aaataaagaa taaacagtat tttaaccaaa aaaaaattct
      781 acttcgctgt tttaattcaa aaaaagatgc taacttgcac gcccttttcc aaaaaacatt
      841 aaaaacagaa aaaaacaaat taacactttt ttttttgatt aaaaatttca tgttattcga
      901 agtcgttaat aacgaacaac ttttatgcaa tccacaaaaa acaaattaaa tcacaactgt
      961 atgaaaaaac tatttctgat actttttgta ttctttttgt gcattccact tttgatggca
     1021 gaaacttcca tcaaaggaac cgtaaagtcg gccgacacac aaaatccgat tcccggagta
     1081 attgtttcct taatagggca gaacatttcg acacaaacca atgcggaagg tcaattctct
     1141 ctgaggttta tcgaagccgg cgatcaggaa attcgtattt ccaaaatcgg ctattttact
     1201 caaatcaagt taattcatat tgaacccgac aaggaaaatg atttgggaac tttttatctg
     1261 cgtgaagatg ttcagcaaca aacaaaacag gaagttgttt tgcaattaag tgaaaatcag
     1321 atagatgaag atgaaggtcg tgctactcaa aatatatcgg ccgctttgtc atccaaaggt
     1381 gatgtatatg tttctcaatc cagctattct ttcagtccta tgcgcttcag aacaagagga
     1441 tatgatgata attttgaatc cacctatatc aacggtgtat attttaacag cttggaaaga
     1501 gggggattca actattcttc gctgggtggg ctgaatgatg ccatgcgaaa tcaggattac
     1561 acctacggtt ttgctccaaa ttccttcagt tatggcaatc tgggaggaac taccaatatc
     1621 aatacaaaag ccagcaacta cgcagccgga acaaaagcca gtgttgccta caccaatcgt
     1681 tcctataaat tgagaggtca ggctacttat gctaccggtt taatgcaaaa tggttgggca
     1741 tttgctggtt cagctgttgt tcgatgggca gacaaaggaa ttgttgacgg cactttttac
     1801 aattcgtggg gatatttctt ttctgccgaa aaaattctta acaaacaaca cagcctgtct
     1861 ttcgtaactt ttggttctcc aactcaacgt gcccaacagg gcgccgttac ccaggaagtc
     1921 tttgatttgg ccggttcaat ttattacaat ccgtattggg gttatcagga aggaaaaatt
     1981 cgaaattcac gtatcgtaaa aagtttcgac ccaaccgcta tcctttctca tgaatataaa
     2041 atcagcaatc atcagttgtt gcgaaccgga ttggcatttc attacagcct ttacagcaac
     2101 tcggctttga atttctacaa tgctcctgat ccacgtcccg attattatcg caatcttccg
     2161 agctttgtta gtgatgattc gttaaaaaat gtgattgcca atcaatggaa atctgacacc
     2221 aatgtttctc aagtcaattg gggtgctctc tatcaagcca attacaaaaa caatgtaatc
     2281 aatcccaacg gaactgcacg atatatcgta gaacgccgac tcaacaacct gatggaagga
     2341 gctttgaact caacctatac ccagcaagtg aataaacagt tgaaacttat tgccggaata
     2401 gaaggtaaaa tgtcgaaagg aatgcattac aaaacacttg acgatttatt gggcggtaat
     2461 caatggattg acatcgatca gttttccgaa cgcgatttcc cttcaaatcc cgatattatt
     2521 cagaatgatc ttagaaaccg aaacgctgtt ataaaggaag gcgacacttt tggttataac
     2581 tacgatatca atatggctta tgccaatggt tttattcaaa atgaatggat acttccacag
     2641 tttgatatct attatgccgc taaactgacc tatacgcaat tctatcgcta cggacacatg
     2701 caaaacggac gtgctcccga aaattcgtac ggaaaaagca aaacttggta ttttgtcgat
     2761 ccttcattta aagccggttt tacttataaa attgacggac gcaatcgttt gtcattcaat
     2821 ggactgatag aaagcagagc gccgctcgcc agccaagctt atctttccga aagaataaag
     2881 gatatgttga ttccttattt gcaaagtcag gatattcggt cggccgatat taactacaat
     2941 tttacctact cgaccattag aggaagaatt ggcgtgtttc agacaaattt atacaacacc
     3001 tccgatatgt tgggatatta tgatgacgaa tacagaactt ttatcaacca tgtgttggca
     3061 aaatcggaca aggtttataa gggagtcgaa ttgggaacaa cggtaaaaat caacagtaat
     3121 ttctccgttt ctttggcagg aacgtacgcc gattatcatt atgccaacaa tgccatcggc
     3181 gtaaaaagtc ctgaaaacgg tgcttttgcc gatgttgccg aaaccgttct tacaaaaggt
     3241 ttaaaaattg ctgcaggtcc tcaagcagcc ggaaatatta ccatcgatta tttccatcct
     3301 aaaatgtggt ttgccgatgt taccttaaat tatttcgaca acaattattt ggattttgcg
     3361 cccaaccgtt tcaccgaaaa caacatggca aaatatacca cacccgaaat gatggctgct
     3421 ttgggcaccc aggaaaaact gaaaggtggt tttctgctgg atgcttctgt aggaaagctt
     3481 atttatttga agcatcgtcg ttctttgaac tttaacttaa gcgccagcaa catactgaac
     3541 aataccaaaa tgattaccgg tggttatcag caagcccgct tgccgctctc caatggagcc
     3601 attgacccca ccggtctgaa cagatttccc aataaatact attatgcttg gggctttaac
     3661 gtgttcttca atat
//
LOCUS       DAOSCH010000147         3664 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_16053_length_3664_cov_4.844278, whole genome shotgun sequence.
ACCESSION   DAOSCH010000147 DAOSCH010000000
VERSION     DAOSCH010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16053_length_3664_cov_4.844278"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(76..1764)
                     /locus_tag="PLZ54_08540"
     CDS             complement(76..1764)
                     /locus_tag="PLZ54_08540"
                     /inference="COORDINATES: protein motif:HMM:NF025943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chondroitinase-B domain-containing protein"
                     /protein_id="HOJ67006.1"
                     /translation="MSFTIVNARQTTVTSASEINNGSWSAGDTIVMKNGTWTNQSISF
                     RGTGNESQPIVLMAETPGNVILNGTSKLQFSGKYIVVSGLYFKDGNLSGSDVISFRTS
                     SSQLAENCRLTNTAIENYNPLDSTVDSKWVSIYGKNNKVDHCSFVNKTNSGTLMVVWL
                     TSGITVNHEISENYFGYRNANLDANGNELNGQEIIRIGDSSTSMTIAAVTVKNNFFEH
                     CNGEIETISNKSCGNTYYNNLFYECKGMLTLRHGNGCTVKGNYFFGNGISNTGGVRII
                     GENHNVYNNYFENLRGTNYRAALCIVRGKENSALNEYFQVKNATVVFNTFVNCSQAFR
                     INYNSSSSLTMPPIGTVIANNHVYNTSSSYYNVSIDLTNATGMDVTWSNNLMNIGRYT
                     NFTYDENQVITGKDAKMIPVSTPIPMYEPSIGSELVNYVTADYPEIINDIRGRERNST
                     AKLPGASEINGTVSSSMPVKTLVGASFYNNPNTGIKNTNTSFKPFTWLIQQHQIIFNS
                     FETGNLYLVDMSGKVLLNWLVPLNTQLITKTLEKGIYLMVFKTPNKQYTEKIIF"
     gene            complement(1920..2954)
                     /locus_tag="PLZ54_08545"
     CDS             complement(1920..2954)
                     /locus_tag="PLZ54_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67007.1"
                     /translation="MKKLILLLSLCVAIFTANAQIFTEYFQTAPIGGNLEGYNDWHVS
                     PKSSEAYGVSPKIAEGALFYTGYAGSNIGNVAVLDSAVGATSATQRISTKIVKFGSDT
                     LKAVVGEKIYTAFLVNISSQSYRSYRDFFTYEGSKTSSMTRGRVFAKVSADGSELFVA
                     VTKNSSTAADYVESTSIPGLTLYPDVNHLFVLVYETIAGDSNDKITLYINPDLTKSEA
                     EQTNKLVAIDTQTDYSATAGLGINLRQRGISAQIGGIRVGKSWDKVLVEGANAVNTPK
                     NSSATIWTSGKNILTNTRGTIKIFNLAGAELISAQTDGCLTTNLSKGLYIARFNGIDG
                     HTAVSKISLN"
     gene            complement(2987..>3664)
                     /locus_tag="PLZ54_08550"
     CDS             complement(2987..>3664)
                     /locus_tag="PLZ54_08550"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HOJ67008.1"
                     /translation="RNNGKGRGEGVLDSYSFVEMLDAVELLKSSKKFPKEDQTALKDW
                     FSSYLNWMLTSEIGQEEYNAKNNHGTAFDVQVVRYALFTENNEVADKFVKTFPERRIF
                     SQIEPNGAQPLELQRTKAFGYSVYNLTHFMDMAFIAKSMNQDIFHAISNDGRSITKAI
                     EFLLPYLGKSPTEFPYQQIEEWDKVQIDFCWVLKRVDIPMGTDNYKNFYSKYLPDNGK
                     DLNFLIY"
BASE COUNT         1101 a          700 c          628 g         1235 t
ORIGIN      
        1 gtcgacaaaa ttactgtttt aattgcagta tccaaatttt tttaaatatt ttaattcctc
       61 ttgtgtttta cttcttcaaa aaataatttt ttcggtgtat tgtttgttcg gagttttaaa
      121 caccatcaag taaatccctt tttctaaggt tttggtaata agttgtgtgt taagtggaac
      181 caaccaattt aacagtactt ttcccgacat atcgaccaaa tatagatttc cagtctcaaa
      241 actattgaaa attatctgat gttgttggat aagccatgta aatggcttga atgaagtatt
      301 ggtatttttt ataccggtgt ttgggttatt ataaaaagaa gcacctacta aagttttaac
      361 tggcatggat gaagaaactg taccatttat ttcgcttgca cccggcaatt ttgcggtgct
      421 gtttctttct ctgcctctta tatcgttgat gatttctggg taatcggccg ttacataatt
      481 taccaattcc gaacctattg atggctcata catgggaata ggagtactta caggaatcat
      541 ttttgcatct ttacctgtta ttacttgatt ttcgtcataa gtaaaatttg tgtatcgtcc
      601 aatattcatc aaattgtttg accaagttac atccattcca gttgcatttg tcaaatcaat
      661 gcttacgttg taatacgatg aagaggtatt ataaacgtgg ttgttggcaa tgaccgttcc
      721 gattggaggc atagtcagtg atgagctgct gttgtaattg attctgaaag cctgtgagca
      781 gttgacgaaa gtattgaaaa ctacggttgc attttttact tgaaaatatt cgttcagtgc
      841 cgaattttct tttccacgaa caatgcaaag tgctgcccga taatttgtac cacgcaaatt
      901 ttcaaaataa ttattataaa cgttgtgatt ttctccaata attcttaccc ctccggtatt
      961 agaaattcca ttgccaaaaa agtaatttcc tttcacggta catccattcc catgacgtaa
     1021 tgtaagcata cctttgcatt cgtaaaaaag attgttataa tacgtatttc cgcacgattt
     1081 attcgaaatc gtttcaattt cgccattaca atgttcaaaa aaattattct tgactgttac
     1141 tgcggcgatt gtcattgagg tagagctatc gcctatgcga atgatttcct gtccgttcag
     1201 ttcgttgcca ttcgcatcga gattggcatt tcgataccca aaataattct ctgaaatttc
     1261 atgattcact gttattccac tggtaagcca aactaccatt aaagttccgg aattggtctt
     1321 atttacaaat gaacaatgat ccactttatt gttttttcca taaatagaaa cccatttgct
     1381 gtcaacggta ctgtctaaag gattataatt ttctatggct gtattcgtta gtctgcaatt
     1441 ttcggcaagt tgagaagaag aggttctgaa tgaaataaca tccgaaccac tcaagtttcc
     1501 atctttgaaa taaagtccgc tgacaactat gtatttaccg gaaaattgga gttttgaagt
     1561 tccattcaaa attacgttac caggagtttc tgccatcagt acaattggtt gcgattcatt
     1621 cccggtacct ctgaaagata tggattgatt ggtccatgtg ccgtttttca taacaatcgt
     1681 gtctcccgcc gaccaactgc cattgttgat ttccgaagct gaagtaaccg tcgtttgtcg
     1741 agcatttacg atggtaaaac tcataaagaa agttattagt aaaaatagtc tgccgcttgg
     1801 gttcgatgaa gttaaaatca taaaattttt ttaaataggg aaaacagatt gtttttttct
     1861 gttttccctg ataaaatggt taagcaatgg gaaaaaatta aatatccata gaattaaact
     1921 caattcaaag aaatttttga tacagccgta tgtccatcaa taccgttaaa ccgagcgata
     1981 tataaacctt ttgacaggtt ggtcgtcaag cagccatccg tttgggctga aattaattct
     2041 gctccggcaa gattgaatat ttttattgtt cctcttgtgt tggtcagaat atttttaccg
     2101 ctggtccaaa tggtagcgct actatttttg ggtgtattga cagcattggc gccttccaca
     2161 agaactttat cccatgattt tccaacccgt atgccaccaa tttgagcact aattcctcgc
     2221 tggcgcagat tgattcccaa accggcagtg gcactataat ctgtttgagt atctatagca
     2281 accaacttgt tggtttgttc ggcttccgat tttgttaaat ccggattgat gtacagcgta
     2341 attttgtcat tgctatcacc tgcgattgtt tcataaacta aaacaaataa atgattaaca
     2401 tcgggataca aagttaatcc cggaatagaa gtagactcta cataatcagc agcagtagaa
     2461 gaattctttg taacagctac aaaaagttct gagccatctg cacttacttt tgcaaaaact
     2521 cttccacgtg tcatactgga agtttttgaa ccttcataag taaaaaaatc gcggtaagag
     2581 cgatagctct ggctggaaat attgaccaaa aatgccgtgt agattttttc tccaactaca
     2641 gctttcaaag tatcgctccc aaattttaca atttttgtag agattctttg tgtagcgctt
     2701 gttgcaccca ctgcagaatc caatactgct acattaccaa tattagaacc tgcataaccg
     2761 gtgtaaaaca aagccccctc agcaattttg ggactcacac cgtaagcttc tgaagattta
     2821 ggagaaacat gccaatcatt atacccttcc aaattaccac caataggtgc agtttgaaaa
     2881 tactccgtaa agatttgtgc atttgccgta aaaatggcaa cacagagcga aagtagtaaa
     2941 attaactttt tcataatgaa taaatttaaa agtgaaacat tattatttaa taaatcaaaa
     3001 aattcaagtc ttttccatta tccggcaaat atttagaata aaaattttta taattatctg
     3061 ttcccatcgg aatatcgact cttttcaata cccagcaaaa gtctatttga actttatccc
     3121 actcttcaat ttgctgatag ggaaattcag taggagattt tcctaaataa gggagcaaaa
     3181 attctattgc ctttgtgata cttcttccat cgttagaaat agcatgaaaa atatcttgat
     3241 tcatggattt tgctatgaaa gccatatcca taaaatgggt cagattatat accgaataac
     3301 caaatgcttt tgttcgttgt aattctaagg gttgtgcacc attgggctct atttgggaga
     3361 aaatccttct ttccggaaaa gtttttacaa atttgtccgc aacttcgtta ttttctgtaa
     3421 ataaagcata tctcactact tgcacgtcaa aagcagttcc atgattgttt ttggcattat
     3481 attcttcctg accgatttcg gaagtcaaca tccagttcaa gtacgacgaa aaccaatctt
     3541 tcaaagccgt ttgatcttcc ttcggaaact ttttggaaga tttcagcaat tcaaccgcat
     3601 cgagcatttc gacaaatgaa taactgtcca agacaccttc tccgcgcccc tttccattgt
     3661 tgcg
//
LOCUS       DAOSCH010000148         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_16280_length_3626_cov_4.937553, whole genome shotgun sequence.
ACCESSION   DAOSCH010000148 DAOSCH010000000
VERSION     DAOSCH010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16280_length_3626_cov_4.937553"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..2048
                     /locus_tag="PLZ54_08555"
     CDS             291..2048
                     /locus_tag="PLZ54_08555"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOJ67009.1"
                     /translation="MTFKELNLKDELLSAIEELGFEQPMPIQERTIPFMQEKSADLVA
                     LAQTGTGKTAAFGLPILNMLDAAQKQTQALILAPTRELCIQISNDLKNYSKYLKGIKV
                     VAVYGGEDIRTQLRQLDTPPQIIVATPGRLIDLTERGKVFLQNIHYLVLDEADEMLNM
                     GFKEDIETILKQTPENRRTMLFSATMPKEISNIVHQFMKDYEEIIVGQKNSGATNVEH
                     IYYLTPAKYRYLTLKRIVDLNPDIYGIVFCRTRQETKEVAEKLMQDGYNADALHGDLS
                     QAQRDNVMQKFRIRNVQLLVATDVAARGLDVSDLTHVINYNLPDDPEVYTHRSGRTGR
                     INKKGISISIIHSKEKHKIKELEKMLKKDFIQQQIPNGLEVCKKQLYHLINRMEHVDV
                     NEEQIQSYLPQIMKQLEYLSKEEIIKRFVSLEFNRFLDYYKDAEDLNEIHFYEDRNNK
                     SKNEKFSKKSGKRIRLKLNVGGREGIDTRRFLGIFNEVVGDKSINVGSIEITNKFTFF
                     DVYADQIDKVMNAFALEPQMDVCEVKGNKQFFEEKPSKFSNHSKKRETYPNINEGSGR
                     KERTANSEKPWRQSQRTRN"
     gene            complement(2290..3570)
                     /locus_tag="PLZ54_08560"
     CDS             complement(2290..3570)
                     /locus_tag="PLZ54_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOJ67010.1"
                     /translation="MDSYQFSFITQLDSTPKFPEISDDSLLTLVQKQTFRYFWDYGHP
                     VSGLARERSGSGETVTIGGSGFGVLATLVAIERSFITRQQGFERLSTIVNFLNTKADR
                     FHGVFAHWMNGTSGKVQPFSEKDNGADLVETAFMMEGLLTVQQYFKNGNREEKNMCDT
                     IQKMWEDVEWNWFQQNGQQKLYWHWSPNYGWAMNMPVTGWNEALIIYILAAASPTHSI
                     EKTVYDNGWARNGAIRNGKTFYDILLPLGEDRGGPLFFTHYSFLGLDPRILSDAYANY
                     WTQNTAHAQINYRYCVANPKKNFGYSRDCWGLTASDAPNGYTASSPNNDNGTIAPTAA
                     LSSFPYTPEESMQALHFFYYILGDKIWGEYGFMDAFNLNKQWFSNSYLAIDEGPIIGM
                     IENYRTGLLWNLFMQNEDVQRGLEKLEFDVNRIF"
BASE COUNT         1203 a          714 c          662 g         1047 t
ORIGIN      
        1 aacccacatg tctgaatatt atattcattc gtgtttgtgt atgcaataca tgtgcgtttc
       61 atgacaaact atagaacaaa aacaaaatcg acaataccat acaaatatga aaataaatga
      121 ctatctttgc acccgaatta aaaaacaact atacgtttcg agtaaatatc cgaaattcaa
      181 gacattatat ttttgatttt tgaaatctgc atcttaaagt acacgtttta tctttcaata
      241 aaatttttac atcggatgaa tgctcaaaaa cgtgtattaa aaaaattaaa atgacattta
      301 aagaattgaa tttaaaagac gagcttcttt cggctattga agagctcgga ttcgagcaac
      361 ccatgcccat tcaggaacgt accatccctt ttatgcagga aaaatctgcc gatttggtgg
      421 ctttggcgca gacagggaca ggaaaaacag ctgcttttgg ccttcctatt ctcaacatgc
      481 tggatgctgc tcaaaaacaa actcaggcac tgattttggc tcctaccaga gaattatgta
      541 ttcaaatcag caacgatctg aaaaattact ccaaatattt gaaaggaatc aaagttgtag
      601 ctgtttatgg tggagaagat atacgtaccc aattaagaca gcttgataca ccacctcaaa
      661 ttattgtagc tactccgggc cgattaatcg acctcaccga acgcggaaaa gtttttcttc
      721 aaaacatcca ttatttggtg ctcgatgaag ctgacgaaat gctgaacatg ggttttaaag
      781 aagatattga aaccattctg aaacaaacgc ctgaaaacag acgtaccatg cttttttcag
      841 ccaccatgcc caaagaaatt tcaaatatcg ttcaccaatt catgaaagat tatgaagaaa
      901 taattgtggg ccaaaaaaat tccggagcta ccaatgtaga acatatttac tacctcactc
      961 cggccaaata tcgttatttg actttaaaac ggattgtaga cctcaatccc gacatttatg
     1021 gcattgtgtt ttgcagaacc cgtcaggaaa caaaagaagt tgccgaaaaa cttatgcagg
     1081 atggttataa tgccgatgct ttgcatggag atctttctca agcacaacgc gacaatgtga
     1141 tgcaaaaatt cagaattcgc aatgttcagc tgctggtggc taccgacgtt gctgccagag
     1201 gattggacgt gagcgatctc acgcatgtca tcaattacaa tttgcccgac gaccccgaag
     1261 tgtacacaca ccgaagtgga agaacaggca gaatcaacaa aaaaggtatt tccatttcca
     1321 tcatccattc gaaagaaaaa cataaaatta aggaactgga aaaaatgctg aaaaaagatt
     1381 ttattcagca gcaaataccc aatggtttgg aagtttgcaa aaaacaactt tatcatttga
     1441 ttaatcgaat ggaacacgtg gatgtaaacg aagaacaaat tcaatcttac cttcctcaga
     1501 taatgaagca attggaatat ctgagtaagg aggaaattat caaacgcttt gtttcattgg
     1561 aattcaaccg ttttctcgat tattacaaag atgcggaaga tctgaatgaa attcattttt
     1621 acgaagatag aaataacaaa tcgaaaaacg aaaaattttc aaagaaaagc ggaaagcgta
     1681 ttcgtctgaa attgaatgtg ggaggacgcg aaggaattga tacccgccgt tttttgggaa
     1741 ttttcaatga agtagtcgga gacaaatcca tcaatgtagg gtcaatagaa attacgaata
     1801 agtttacatt tttcgatgtc tatgccgatc agatagataa agtaatgaat gcgtttgctt
     1861 tagagcctca aatggacgtt tgtgaagtga aaggaaacaa acaatttttt gaagaaaagc
     1921 cttcgaaatt ctccaatcat tccaagaaaa gggaaactta tcccaatata aatgaaggat
     1981 ccggaaggaa agaaagaacc gcaaattccg aaaaaccgtg gcgacaatcc caaaggacac
     2041 gtaattaaat ttttgatttt tttcctacaa ttcctgttca cctaaaaaat ttgttttatc
     2101 ttcgtaactt ttttaaaaga aagagaccaa agtaaagcct ttattccatc aagacataag
     2161 gagggaatgt ttttcctcct ttgtcttgca aaataatttt ttgtttacct tttgcctccc
     2221 ccctgaaaaa gttgcattaa ttacttaaat ttacgtttca taatttcaat taccctgttg
     2281 aaaaattact caaaaaatcc tgttaacgtc aaactccaat ttttccaatc ctcgttgtac
     2341 atcttcattt tgcatgaata aattccaaag taatccggtt cggtaatttt caatcattcc
     2401 gataatggga ccttcgtcta tggctaaata ggaattggaa aaccattgtt tgttcaaatt
     2461 gaacgcatcc ataaaaccat attcgcccca gattttatct cccaatatat aataaaagaa
     2521 atgtaaggct tgcatactct cttcaggtgt gtagggaaag gatgacaaag ctgccgtagg
     2581 tgcaatagtt ccattgtcgt tattcggtga ggaagcagta taaccgtttg gagcgtcgct
     2641 ggcagtcaga ccccagcaat ctcggctgta accaaaattt tttttcggat tggctacgca
     2701 atagcgataa ttgatttgag catgagctgt attctgcgtc caataattgg cataagcatc
     2761 cgataaaatt cttggatcaa gtcccaaaaa tgaatagtga gtaaaaaata gcggaccacc
     2821 tcgatcttca cccagtggaa gcagaatgtc gtaaaaagtt tttccgtttc gtatagctcc
     2881 gtttcgtgcc cagccattgt cataaactgt tttttcgatt gaatgagttg gagacgcagc
     2941 tgccagaata tagataatca gagcttcgtt ccaaccggta acaggcatat tcattgccca
     3001 accatagttg ggcgaccaat gccaataaag tttttgttgt ccgttttgtt gaaaccagtt
     3061 ccattccacg tcttcccaca ttttttgaat ggtgtcgcac atattttttt cttcacgatt
     3121 tccattttta aaatattgtt gaaccgtcaa taaaccttcc atcataaaag ctgtttccac
     3181 caaatcggcg ccattgtctt tttcgctgaa aggctgcact tttccgcttg tcccgttcat
     3241 ccagtgagca aaaacgccgt gaaaacgatc ggcttttgta ttcagaaaat tcacaatggt
     3301 ggataatctt tcaaagccct gttggcgggt aataaaactt ctttcgatag ccacaagcgt
     3361 tgccaataca ccaaaacctg aacctccaat ggttactgtt tcgcccgaac cactacgttc
     3421 tcttgcaagt cctgaaacag gatgtccgta atcccaaaag tatcgaaacg tctgcttttg
     3481 aaccagtgtc aacaagctgt catcggaaat ttcagggaat tttggagtgg aatcgagttg
     3541 ggtaatgaaa gagaattgat aactgtcaat taaattgacg cccaaatttt ctccttccgc
     3601 aatataaagc gtatagcggg ttaaat
//
LOCUS       DAOSCH010000149         3582 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_16566_length_3582_cov_3.849447, whole genome shotgun sequence.
ACCESSION   DAOSCH010000149 DAOSCH010000000
VERSION     DAOSCH010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16566_length_3582_cov_3.849447"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(330..>2438)
                     /locus_tag="PLZ54_08565"
     CDS             complement(330..>2438)
                     /locus_tag="PLZ54_08565"
                     /inference="COORDINATES: protein motif:HMM:NF019368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HOJ67011.1"
                     /translation="AWNDEFIAANEFAEVLKHGVSTVAAKLNTQTRGRAIVVYNPVAK
                     AREDVVEAELEYAVLPESVGVFDKSGKEVPSQIVEKKGNKLKILFLAEMPSAGLKIYD
                     VRETASSVKSTGLSITNNALENAYYRVKVASNGDIISIFDKKASKELLAKPARLEFLA
                     ETPNEWPAWNMDWKDRKKPPIDFMDKDTQLTIVEQGPVRVTLKVQRKGRNSEITQFYS
                     LAAGEAGKRLEVSNKIDWQSRAVSLKASFPLTVSNENATYNLGVGTISRSNNNEKKFE
                     VPSKEWFDLTDKSGKYGVTILEDCKYGSDKPDDNTLRLTLLYTPGIHLPDWQWTMYQS
                     TQDWGIHDVMYAVYGHNGDWRKGESAWQAQFFNQPLLAFESTKHGGEQGGSISMISIN
                     RPEAGLMALKKMESGEGYVIRINELTGKNIKNIQVMLPAEIVDAYEINGQEKKIGDVK
                     FTKQGISFDLSPYTIRSFAVKVKGMPNVSIPQQTVEILYNTDVISSDENREDGTMFNG
                     QSLPTELLPEVVESEGIRFKIGNKEDEQNNAVVCTGQTIPLPEGKYTKLYLLAAAEND
                     SWADFVVDGQTVSLGIQRWTGYVGQFYNRVLSADQNKVVEMKAPYAKTDNIAWFASHC
                     HNNYPMKNQAYQYCYLYKYEIPIPVGAKNITLPNNKDIKILALTVVAPESEELKPLQP
                     LYDHFDKNPLFELREKQKFY"
     assembly_gap    2439..2448
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2449..>3582)
                     /locus_tag="PLZ54_08570"
     CDS             complement(<2449..>3582)
                     /locus_tag="PLZ54_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HOJ67012.1"
                     /translation="IFYLLPLWLTAASMLQAQPWLEPSDYDITKDRLLYTVGYAHLDT
                     EWNWDYPTTINEYILNTMVENLNLFEKYPDYVFNFTGSRRYKMMKEYYPKLFDKVKNY
                     VAQGRWKVSGSSVDEGEVNISSSESLVRQVLYGNNFFRSEFGKESMDYMLPDCFGFSW
                     NLPSILHHCGLIGFSTQKLTWHSAAGVPFNVGIWKGPDGKGVIAALNATSYTSNVENR
                     LDKDSKWISRLNEDKEKTGYAFDYRYYGVGDQGGAPRMNDVRHAVNSLPREDSNFKVL
                     LTSSDQMYKDITPEIKEKLPVYSGDLLLIEHSAGSLTSQAFMKRMNRKNELLASSAEM
                     ASVAADWMGGASYPFEKLNHAWDLLLGSQFHDILPGTAIPKAY"
BASE COUNT         1065 a          813 c          583 g         1111 t
ORIGIN      
        1 tcttcctttt tcatccgtaa ataattttat taagtcataa attcaagtaa ttaatataac
       61 tttttctggg ggaataaaag gtaaaggaaa attatttttc aagacaaagg agaaaaaaga
      121 gctttcccct ttgccctgat ggaataaatg ctttactttg gtcttttccc cttaaaaagt
      181 tacaaagatg aaacatttaa ccgtaaaaca aaagtacaca atttgtgtaa tgaaccaaca
      241 cggatacaaa caaaaaatat agctttaact attggaaagg ataaatcaat tgtatccaga
      301 gattcaattg ttgagttaaa tgtggataat caataaaatt tttgtttttc ccgtaattca
      361 aaaagtggat ttttgtcaaa atgatcatac aatggctgca gcggtttcaa ctcctcactt
      421 tcaggagcaa caaccgtcaa agcgagaatc ttaatgtctt tattattcgg cagagtaata
      481 ttttttgctc caacagggat tgggatctcg tatttataca gataacagta ttgataagct
      541 tgatttttca ttggataatt gttgtgacaa tgggaagcaa accatgcaat attatcggtt
      601 ttagcataag gcgctttcat ttcaaccact ttattttgat cggcacttaa tacgcgatta
      661 taaaattgtc ctacatatcc cgtccatcgt tggattccta acgaaacagt ttggccgtcc
      721 acaacgaaat ctgcccagct atcattttcc gctgctgcca gcaaataaag tttcgtatac
      781 ttcccttccg gtaaaggaat agtttgaccg gtgcaaacta cggcattatt ctgctcatcc
      841 tctttattgc cgattttgaa acgaatgcct tcactttcta ctacttctgg aagcagttcg
      901 gtgggaagcg actgcccatt aaacatcgtt ccgtcttccc gattttcatc ggaactgatc
      961 acatcggtat tataaagaat ttccacagtt tgttgaggaa tcgaaacatt aggcattcct
     1021 tttactttta ctgcaaaact tcgaatcgta taaggactca aatcaaacga gattccctgt
     1081 ttggtaaact tcacgtcacc tatttttttt tcctgtccgt tgatttcgta agcatccact
     1141 atttcagcag gtaacattac ttgaatattt ttgatgtttt ttcctgtcag ttcgtttatc
     1201 cgaatgacat aaccttcacc tgattccatt tttttcaacg ccatcaaccc tgcttcaggc
     1261 cgattaatgg aaatcatcga tatagatcca ccctgttctc ccccatgttt tgtcgactca
     1321 aaagccaaca aaggttgatt aaaaaactgt gcctgccatg ccgattctcc ttttcgccaa
     1381 tcaccattat gtccatatac tgcatacatt acatcatgaa tcccccaatc ttgggtactt
     1441 tgatacatgg tccattgcca atcaggtaaa tgaattcccg gagtataaag taaagttaat
     1501 cgcaaggtat tgtcatccgg cttatctgaa ccgtatttgc aatcttccaa aatggtcacc
     1561 ccatatttac ctgatttatc agttaagtca aaccactctt ttgaaggcac ttcaaatttt
     1621 ttctcattat tattgcttct tgaaatagtt cctactccca aattataggt cgcattttca
     1681 ttagaaaccg tcaaggggaa agaagctttt aaacttactg cacgcgattg ccaatcaatt
     1741 ttgttactga cttccaatcg ttttccggct tctccggcag ctaaactgta aaattgggta
     1801 atctcggaat ttctaccttt tcgctgaact tttaaggtca cccgcaccgg tccctgctca
     1861 acaatagtca actgagtatc cttatccata aagtcaatag gcggtttctt gcgatctttc
     1921 caatccatat tccatgccgg ccattcattg ggtgtttcag ccaaaaattc caaacgagca
     1981 ggtttcgcca acaattcttt agaagctttt ttatcgaaaa tgctgataat atccccattc
     2041 gaagccactt ttactcgata atacgcattc tctaaggcat tgttcgtgat agaaagccct
     2101 gtcgacttta cagaagaagc tgtctcacgc acatcatata tctttaagcc tgctgaaggc
     2161 atctcggcca aaaacaatat tttaagttta tttcccttct tctccacaat ttgagaagga
     2221 acttctttgc cacttttatc aaaaacccca accgactcag gtaaaacagc atattccaat
     2281 tcagcttcca ctacatcctc cctggcctta gctaccggat tatacactac aatagctctc
     2341 cccctcgttt gggtattcaa tttggcggct acagtactca ccccatgttt aagcacctca
     2401 gcaaattcat tggccgcaat aaattcgtca ttccatgcnn nnnnnnnncg tatgcttttg
     2461 gaatggccgt tcccggaaga atatcgtgaa actggcttcc caacaacaaa tcccatgcat
     2521 ggttcaattt ctcgaaagga taagaagctc ctcccatcca atccgctgca acggatgcca
     2581 tttcagcaga ggaagcgagc agttcgtttt ttcggttcat ccgtttcata aaagcttgcg
     2641 aggttaacga acccgcactg tgttcgatta gcaacaaatc gccagaataa acgggaagct
     2701 tctccttgat ttcaggggta atatctttgt acatttgatc ggaagaagtt aacaacactt
     2761 tgaaattgct atcttcccga ggcaaactat ttacggcatg ccttacatca ttcatacggg
     2821 gagcaccacc ctgatcacct actccataat agcgataatc aaaagcataa cccgtttttt
     2881 ctttatcttc atttaaacgg ctaatccatt tagaatcctt atccaaccga ttttcaacat
     2941 tggatgtgta gcttgttgca tttaaagcag caataacccc tttgccgtca ggacctttcc
     3001 atatacctac attaaatggc acgcctgcag ccgaatgcca tgtgagtttt tgggtagaaa
     3061 aaccaatcaa gccacaatga tgcaaaatgg atggtaaatt ccatgaaaat ccaaaacaat
     3121 caggcaacat gtaatccatg ctttctttcc caaattcact gcgaaaaaaa ttatttccgt
     3181 aaagtacctg ccgtactaat gattcagaag aagaaatatt gacctcaccc tcatccacgg
     3241 aagaaccgga aactttccaa cgtccctgtg ccacataatt ttttacttta tcaaataatt
     3301 tgggataata ttccttcatc attttatagc gacgtgaacc tgtaaaattg aaaacgtaat
     3361 caggatattt ttcaaaaagg ttgagatttt ctaccatcgt attcaaaata tattcattaa
     3421 tggtagtagg ataatcccaa ttccattcgg tatccaaatg agcatatccc accgtgtaca
     3481 gcaaacgatc ctttgtaatg tcgtaatcac tcggttctaa ccagggctgc gcctgaagca
     3541 tggatgctgc agtcaaccat agcggcaaca aataaaaaat tt
//
LOCUS       DAOSCH010000150         3432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_17553_length_3432_cov_5.896950, whole genome shotgun sequence.
ACCESSION   DAOSCH010000150 DAOSCH010000000
VERSION     DAOSCH010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17553_length_3432_cov_5.896950"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..1673
                     /locus_tag="PLZ54_08575"
     CDS             273..1673
                     /locus_tag="PLZ54_08575"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOJ67013.1"
                     /translation="MNKRKLLYRYLISDTLASLVVWVVFMIFRKIVNDAAIFENVQIF
                     FPNYNYLSSLILFPLSCITVHYLSGFYLYPEKHSGIKIFFTTFVASLIISIVIFFVLM
                     LDDIVVSYKYYYYSLAVLFGLLFVFTYLPRLIIYSNVKSNYRSKKWTIPTVIIGTGQN
                     AIKIGNELAKAAPEKTVVGYVETNHGKLISQEMILGNLHQIEKIIEKYNIQEAIIALD
                     QTDDYQLFSIINSLYRFNIDIQFTPRLYEILTGSARIDNLSISPLVSITDIKMPDWEQ
                     SVKRTFDIGFSLLALVLLSPVLLYFMIRIKTDSKGPVFYLQERIGRYGRPFKIIKFRT
                     MFEGAENGIPRLSNPNDNRITNIGKKLRKYRIDEIPQFINVLLGHMSIVGPRPERPYY
                     IQQIVQEAPYYCLLYKIRPGLTSWGPIRIGYSDSIEKMIERLNYDIIYLESMSLITDL
                     KILLLTLEIIFKGKGV"
     gene            complement(1765..3033)
                     /locus_tag="PLZ54_08580"
     CDS             complement(1765..3033)
                     /locus_tag="PLZ54_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOJ67014.1"
                     /translation="MKFFLRLNKLLSEISAKGFFHLLSANVLIQLVAFASQLFVAGIL
                     LPEDVGRIKIITTFLSVFSIIGGMGLHASTLKLCSENRSQKEIRELFSSGVVFTIVST
                     IVFYGLALVLNSFGFFSSDAIIRRLIPLGLFPLITNSAFMVLVAYFQASKQIKIMSRI
                     TVANKLIAIVAIVLFTWWFGINGYYVAYNLSFIVMFFVGFRLLRKDFTFSKDRESVKH
                     NFKTHLVYAKPSLMSNLLSEISAYADIFVINYLLKDMKEIGYYSFALTLTVVLRVFPF
                     TVQQIASPYFSGLAHDRSHYLQVFKKYNRQLYLVVGVTLLLAILAGPFLIRIIFAGKY
                     DPAIPYFVLLSVGWSIRLLNQLQSAAIFGLGKIQYNAWSSFISLIFNVAVYIVFVRLY
                     GVIGAAWASIPAGVVMTLTSGLFLRKAMRE"
BASE COUNT         1184 a          621 c          636 g          991 t
ORIGIN      
        1 cgatttttaa acgcttttct cacttgtttt tcggctcacc ggacaaggtc aacccaagtg
       61 cttggctgat atgtcgtgtt ttacagatat ataaaacaaa tatttcaatt tttgccatgt
      121 attaaagtaa taattataaa gcggtgaaaa gaaaataaag aatactaatt ttcgtttata
      181 aaaaattgag agatgataag tgtcgcaaaa tattgaaaac aattataaag taatagaatt
      241 caaaatttag aaatttcctc atcagcgagc ggatgaacaa gagaaaactg ttatatcgtt
      301 atttgatttc ggatacactg gcttcactgg tggtgtgggt agtgttcatg attttccgaa
      361 aaatagtgaa tgatgcagct atttttgaaa atgttcaaat tttctttccg aattacaatt
      421 atctttccag tctgatactt tttccgttga gttgtattac agttcattat ctttccggat
      481 tttatcttta tcctgaaaaa cattcaggca taaaaatatt ttttaccact tttgtagctt
      541 cattgattat ttccattgtc attttttttg tgttgatgct cgacgatatt gtggtatctt
      601 acaaatacta ctattattct ctcgccgtgt tgttcggatt gttgtttgtg tttacttatt
      661 tgcctcgtct tattatttat tccaatgtta aaagcaacta tcgttcaaaa aaatggacta
      721 ttccgacggt tattattgga acgggacaaa atgccatcaa aatagggaat gagctggcaa
      781 aggcagctcc tgaaaaaact gtggtgggat atgtggaaac taatcatgga aagctgatat
      841 cacaggaaat gatacttggc aatttacatc aaatagaaaa aattatcgaa aaatacaaca
      901 tacaggaagc cattattgca ctcgatcaaa ctgatgatta tcagcttttt tcaataatca
      961 attccctcta tcgcttcaat atcgatattc agtttacgcc acgactgtat gaaattttaa
     1021 ccggaagtgc ccgcatcgac aatttaagca ttagtccgtt ggtaagtatt accgacataa
     1081 aaatgcccga ttgggaacaa agcgtcaaac ggacattcga cattggcttt tcgctgttgg
     1141 ctttggtctt gctttcgccc gttttgctgt attttatgat tcggattaaa acggattcca
     1201 aaggccctgt tttttatctt caagaaagaa tcggacgtta tggtcggcca ttcaaaatta
     1261 ttaagttccg aaccatgttt gaaggtgcgg agaacggcat tccccgattg agcaatccca
     1321 acgataatcg gataacgaat attgggaaaa agttgagaaa gtatcgcatt gacgaaattc
     1381 cacaatttat caacgtttta ttgggacaca tgagcattgt tggtccgcgt cccgaaaggc
     1441 catactatat tcagcaaata gttcaggaag ctccctatta ctgtctgtta tacaaaattc
     1501 ggccgggact cacctcgtgg ggacctatcc gcattggcta ttccgacagt atcgaaaaaa
     1561 tgatcgaacg gctgaattac gacattatct acttggaaag catgtcattg attaccgacc
     1621 tgaaaattct ccttcttact ttggaaatta tttttaaggg aaaaggagtg taaacttgta
     1681 caaaaatttg aataaaagaa ccaaaatttg tcaaagtttt tcgcctagtc gatgtaaatc
     1741 aataaacaaa aaataccgaa ccagttattc ccgcattgct tttctgagaa acaaacccga
     1801 tgtgagggtc atgacaactc cagccggaat acttgcccat gccgctccaa taacgccata
     1861 caagcgaaca aaaacaatat aaacggcgac gttgaatatc aacgaaataa aactgctcca
     1921 agcgttgtac tgaatttttc ccaatccaaa aatggctgca ctctgaagtt ggttcagcaa
     1981 gcgaatactc cagcctaccg acaacaagac gaaataagga atggccggat cgtattttcc
     2041 cgcaaaaata atcctgataa gaaaaggccc tgccaatatg gccagcagca atgtaacgcc
     2101 aacaaccaaa taaagctgtc ggttgtattt tttaaaaacc tgcaaataat ggctgcggtc
     2161 gtgggcaagt cctgaaaaat agggtgatgc tatttgttgc accgtaaaag gaaaaacacg
     2221 cagcactaca gtcaacgtca atgcaaaact gtagtaacca atttctttca tgtctttcaa
     2281 caaatagttg atcacaaaaa tatcggcata agcagaaatt tcggaaagca gattggacat
     2341 caatgaaggt tttgcataaa ctaaatgtgt tttaaaatta tgtttgacac tttccctgtc
     2401 tttggaaaaa gtaaaatctt ttcgcaacaa ccggaaccca acaaaaaaca taacaataaa
     2461 actcaggttg taagccacat aatagccatt aattccaaac caccacgtga acaacacaat
     2521 ggcaacaatg gcaatcagtt tattggcaac agttatgcgg gacatgattt tgatttgttt
     2581 gctggcctga aaataagcaa ccaaaaccat gaaagccgaa ttcgtaatca gagggaaaag
     2641 tcccagcgga ataagcctgc ggatgatggc atcggaagaa aaaaatccaa aagaattcaa
     2701 tacaagtgcc aacccgtaaa aaacaatggt ggaaacaatg gtaaacacta caccggaact
     2761 gaaaagttcc ctgatttctt tttgtgaacg gttttcggaa caaagtttca aggtagaagc
     2821 atgcaaaccc attcctccga tgatggaaaa aacagaaaga aaagtggtga taatttttat
     2881 tcgtccaacg tcttcgggca aaaggatacc ggctacaaaa agctgcgaag cgaaagccac
     2941 caattgaatc aaaacattgg ccgacagcaa atggaaaaag ccctttgcag aaatttcgga
     3001 taaaagttta ttgagccgga gaaaaaactt catgtttttc atcttttgcc tatattcttt
     3061 ctgacacata catttattta ctaaagtttc gcatttcaga aagggctgaa aataagagag
     3121 aaaaaaaagt aaaaaagtag catattttat ttaatatctc attaaaaatg agtatgttag
     3181 ctgtataatt acaaaacaga aaaaatatgc taccggacaa aattaaggaa aaaatttctg
     3241 aaacaggaac agatttcaat gaaaataaat tagagagcat aaatattttg aaaggtttca
     3301 aggcgttgag actgacagaa ggattttcgg agttcaagca tttgaaaagg agtggttata
     3361 gttttcatca gaatatgatt tattttttaa aggtctgatg gaacccacat gtctgaatat
     3421 tatattcatt cg
//
LOCUS       DAOSCH010000151         3426 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_17588_length_3426_cov_7.021359, whole genome shotgun sequence.
ACCESSION   DAOSCH010000151 DAOSCH010000000
VERSION     DAOSCH010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17588_length_3426_cov_7.021359"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLZ54_08585"
     CDS             complement(<1..190)
                     /locus_tag="PLZ54_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HOJ67015.1"
                     /translation="MRKIVTVVGARPQFIKAATLSRQFPTVGVEEIIIHTGQHFDNNM
                     SDVFFEEMEIPRPAYHLEI"
     gene            complement(178..1479)
                     /locus_tag="PLZ54_08590"
     CDS             complement(178..1479)
                     /locus_tag="PLZ54_08590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOJ67016.1"
                     /translation="MKKVLIITYYWVPSGGAGVQRWVKFTKYLRSFGWEPVVYTPQNP
                     EYPALDHSLEKDVPPDMEVIKTPIWEPYNIYRNLMGKKNEPINAGFISENKKSGWKEQ
                     FSIWIRGNFFIPDPRRFWVKPSVKFLKNYISENQIDAVVTTGPPQSMHLIGLGLKKNF
                     PTLTWLADFRDPWTHIDFYDDLHLTKIADRIHHQLEKKVVTSADVVVVVSKSMKKEFL
                     RLQPKRIEMIPNGFDEEDFRTNKTELDEKFTISHIGTLNAARNPTIFWKVLNELCAEN
                     EQFKSDLLIQLIGKVDFSILEEINENNLKDNFLKIDYLPHNEAIAKQMSSQVLLLLIN
                     QTNNAKGIVTGKLYEYLAARRPILGIGPTNGEVGEILKSSQAGIMIDYIDAKKMKETI
                     LDYYQQYKNHSLTVPSNLVENFSRKNLTRDLANLLDSLCEK"
     gene            complement(1539..3065)
                     /gene="gltX"
                     /locus_tag="PLZ54_08595"
     CDS             complement(1539..3065)
                     /gene="gltX"
                     /locus_tag="PLZ54_08595"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOJ67017.1"
                     /translation="MRRVRVRFAPSPTGPLHIGGVRTALYNFLFARKNNGDMLLRIED
                     TDSSRFVPGAEDYIVESLKWLGITIDEGIGAEKEGAFGPYRQSERKHIYRKYVDQLLN
                     DGKAYIAFDSPIELESKRAEIPNFQYDAQTRLSMNNSLTLPEEEVRKRLENDEQYVVR
                     IKIEPGEDIVVNDLIRGEVSINSSVIDDKVIFKSVDELPTYHLANVVDDYLMQITHVI
                     RGEEWLPSSPLHVLLYRYLGWENEMPQFAHLPLLLKPDGKGKLSKRDGDRLGFPVFPL
                     NWKDPRTGEIYNGFREAGYFPEAVVNFLALLGWNPGTDQEIFSMDELIEQFSLERVSK
                     SGAKFDYEKAKWFNHKYLQMKPDEEVADIFQRILIEKGFIEDTDKVIKIISLIKDRAD
                     FITDLWEQSHYFFEAPEHYDAKAVQKRWKPEIPEQLEELGKMLLSLEAASADEMEKSI
                     KEWISGKNYHLGNLMNALRLALVGELKGPSIFEIVEVLGKEEAARRIKKAIEVLSGES
                     "
BASE COUNT         1041 a          718 c          589 g         1078 t
ORIGIN      
        1 gaatctccag atgatacgcc ggacgcggaa tttccatctc ctcaaaaaaa acatcggaca
       61 tgttgttgtc gaaatgctgt ccggtatgga taattatttc ttcgacacca actgttggaa
      121 attggcgact caacgtggct gccttgataa attgtggacg agctccaaca actgtaacta
      181 tttttcgcat aatgaatcca ataagtttgc taaatcacga gttaaatttt ttcgagagaa
      241 attttccact aaattgcttg gaactgttaa tgaatgattt ttatattgct gataatagtc
      301 aagaattgtt tccttcattt ttttagcatc gatataatca atcatgatgc cagcttgaga
      361 gctcttcaaa atttcaccaa cttctccatt tgttggtcca attcccaaaa tggggcgacg
      421 agcagccaga tattcgtata atttgcccgt aacaattccc tttgcattgt ttgtttgatt
      481 aatcaacaac aaaagcacct gagatgacat ttgttttgca attgcttcat tgtgaggtaa
      541 ataatcaatc tttaaaaaat tatcttttaa attattttca ttgatttctt ctaaaataga
      601 aaaatctact ttgccaatca gttgaatgag taagtcggac ttaaattgtt cattttcagc
      661 acaaagttca ttcaaaactt tccagaatat tgttggattt ctggcagcat tcagggtgcc
      721 aatatgcgaa atggtgaatt tctcgtccaa ttctgttttg ttagtacgaa aatcttcctc
      781 atcaaaacca ttgggaatca tttcaattct tttgggctgt aaccttaaaa attctttctt
      841 catgctcttg gaaaccacga caactacatc ggccgaagta accacctttt tttccaattg
      901 atgatgaatg cggtcggcaa ttttcgttaa atgcaaatcg tcataaaaat cgatatgggt
      961 ccagggatca cgaaaatcgg ccagccacgt taaagttgga aaattttttt tcaatcccag
     1021 tccaatcaaa tgcatggatt gaggaggtcc ggtagtaaca accgcatcaa tctggttttc
     1081 ggaaatatag ttttttaaaa atttcaccga aggctttacc caaaagcgac gtgggtcggg
     1141 aataaaaaaa ttgcctcgaa tccaaatgga aaattgttcc ttccaacccg atttcttgtt
     1201 ttcggaaata aatccggcat tgatcggctc atttttcttg cccatcaagt ttcgataaat
     1261 attgtaaggt tcccaaatag gcgttttaat cacttccata tcgggcggaa catctttttc
     1321 gagcgaatgg tcaagcgcag gatattccgg attttgaggc gtatatacaa ccggttccca
     1381 accaaaactt cgcaggtact ttgtaaactt tacccatcgc tgcacgccgg cacctccact
     1441 cggtacccaa taataagtaa tgatcagtac ttttttcatt cgttatttta agaacctttt
     1501 tactatgaga gcttttttag ctaaaaaact tccgtttatt aactttcacc gctcaacact
     1561 tcgatggctt tcttaattcg tctggccgcc tcttcttttc ccagcacttc aacaatttca
     1621 aaaatggacg ggcccttcaa ttcccctacc aaagccagac gaagagcatt cattaaattg
     1681 ccgagatggt aattttttcc cgaaatccat tccttaatgc ttttttccat ttcatcagcc
     1741 gaagcagctt ccagcgatag caacatttta cccagttctt cgagttgttc aggaatttcg
     1801 ggtttccagc gcttttgcac ggctttggca tcataatgtt ccggtgcttc aaaaaagtaa
     1861 tgagactgtt cccacaaatc ggtaataaaa tcggctcgat ccttgatcag agagataatt
     1921 ttaatcactt tgtcggtatc ctctataaaa cctttttcaa tgagaatacg ttgaaaaata
     1981 tccgccacct cttcatccgg tttcatttgc agatatttat gattgaacca tttggctttt
     2041 tcataatcga attttgcacc acttttgctg acacgctcta gcgaaaactg ttcaatgagt
     2101 tcatccattg aaaaaatttc ctgatcggta ccgggattcc agcccagcaa agccaaaaaa
     2161 tttactaccg cttcaggaaa atatccggct tcgcgaaaac cattgtagat ttcgccggtg
     2221 cggggatctt tccaattgag aggaaaaacc gggaacccaa gcctgtcgcc gtcgcgttta
     2281 ctgagttttc cctttccatc gggtttaagc agcaaaggaa ggtgagcaaa ttgcggcatt
     2341 tcgttttccc acccaagata tcgatacagc aaaacatgca aaggcgaaga cggtaaccat
     2401 tcttctccac gaattacgtg agtgatctgc atcaggtaat cgtccacaac attggccaaa
     2461 tgataggttg gcaattcatc aaccgattta aaaataactt tgtcgtcaat caccgaagaa
     2521 ttaatgctga cttcaccacg aatcaaatca ttcactacaa tatcttctcc gggttcaatt
     2581 ttgatgcgca ccacatattg ttcatcgttc tccaatcttt ttctgacttc ctcttcgggc
     2641 aaagtcagag aattgttcat cgaaagacgt gtctgtgcat cgtactgaaa attgggtatt
     2701 tcagcccttt tactttccag ttcaatgggc gaatcaaaag caatgtaggc ttttccgtca
     2761 tttaaaagct gatctacgta ttttcggtaa atatgttttc tttcgctttg ccgataaggg
     2821 ccaaatgcgc cttccttctc tgccccaata ccctcatcaa tagttattcc caaccatttc
     2881 agcgactcta caatataatc ttcagctccg ggaacaaagc gtgaagaatc ggtatcttca
     2941 atgcgcaaaa gcatgtcgcc attgtttttt cgggcaaaaa gaaaattgta caaagccgta
     3001 cgaacaccac caatgtgcaa aggccccgta ggacttggcg caaaacgaac tctcactctt
     3061 ctcatgaaaa ggaaatttaa tgtttgaagc tttaaaactg atatatgtta tcggtttgca
     3121 aaaataagaa atttaactga gatataaact taaaatgttc attaggagtt taaggaaaat
     3181 aattattttt aaatccaact caaaatctaa gttttcatta acaaccttga gaaactttac
     3241 atctaaaatt aattgatgag ggaattaatt aaaaaataaa agaagagcaa aatagatata
     3301 aaaccggcta aagaaaatct tatagaaaaa aattatatgt tttgcataaa aattttaatt
     3361 tttacaaaat ttttaatgat tatccgcaat gattcctatt gtaaattctt gacttgaaat
     3421 cggagt
//
LOCUS       DAOSCH010000152         3392 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_17834_length_3392_cov_6.863051, whole genome shotgun sequence.
ACCESSION   DAOSCH010000152 DAOSCH010000000
VERSION     DAOSCH010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17834_length_3392_cov_6.863051"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..341)
                     /locus_tag="PLZ54_08600"
     CDS             complement(51..341)
                     /locus_tag="PLZ54_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67018.1"
                     /translation="MDYSQLDMLMTVSKKGDASKDKKNQFNNLLKSYILENGYTDQAE
                     KYLFDGFSFCGATPIFEIMINKTNTDDRLAIFMSISNGKLFCKNEKGITFKL"
     gene            complement(375..1493)
                     /locus_tag="PLZ54_08605"
     CDS             complement(375..1493)
                     /locus_tag="PLZ54_08605"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpaII family restriction endonuclease"
                     /protein_id="HOJ67019.1"
                     /translation="MTTQTNAKNKGEWSELYAFIKLLKEGRIYAADENVNRLDNIYLP
                     IIKIIREEVKNEKIDYYTGDIIRIYKNNCLLEEIPANELDVPTRTLFEKIFEGSRNSQ
                     SSGAFTIEEAFSFMERMHIRKVKAPSTDKIDLTMQIHDIYTGYSPEVGFSVKSDVGSP
                     PTLLNAGKNTRIQYKVMGLNDLQIEEINNIDETVDRNYMVMRMSRLFALAENVSFEKV
                     KDQTFNDNLIMIDSLLPRIYGEMILKHYSEIESGIYDCDTLVTYLTEANPMHYHNPNV
                     YKYKIKKLITASALGMTPGKEWDGLDSATGGYIIIKRDGDVLCYHLYNRNFFEEYLLK
                     NTRFDRPSASRYDYGYLYNENGQVFIDLNVQIRFKSIK"
     gene            complement(1465..2490)
                     /locus_tag="PLZ54_08610"
     CDS             complement(1465..2490)
                     /locus_tag="PLZ54_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004346767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOJ67020.1"
                     /translation="MKQLRSIDLFAGIGGIRLGFDQAFGERIKTVFISEWDEYAQKTY
                     KSNFEDEIEISGDITQIDEKDIPQFDICLAGFPCQAFSLAGKRMGFNDDYKGKSRGTL
                     FFDVARICDYHKPKIIFCENVKGLTIHDKGRTFKIITETLRELGYTVYSEILNSKDFG
                     VPQNRERIYIVAFRNDIDSSNFTFPAPINKKVCIKDIIEENPVSVKYYLSTQYLNTLI
                     NHKKRHESKGNGFGYEIRDLNGIAGAIVCGGMGKERNLIIDDRLTDYTPVTHIKGEVN
                     RQGIRKMTPREWARLQGFPDTFKLPLADVHLYKQLGNSVTVPVIKAIATKIKEVLDHD
                     NANQCKE"
     gene            complement(2487..2897)
                     /locus_tag="PLZ54_08615"
     CDS             complement(2487..2897)
                     /locus_tag="PLZ54_08615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HOJ67021.1"
                     /translation="MSKKTREQIKYNMSRVKNKNSKIELMLRKELWSRGLRYRKNVTD
                     IFGKPDIVFKGKKVAVFCDSEFWHGYNWEERKHDFKSNQDFWIPKIERNMQRDIEVTQ
                     TLEFQGWTVLRFWGNDIKKNLASCADAIEKAVRK"
     gene            complement(2923..3123)
                     /locus_tag="PLZ54_08620"
     CDS             complement(2923..3123)
                     /locus_tag="PLZ54_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOJ67022.1"
                     /translation="MDLLTAKQASEKWGISARRVTRLCEEGRIEGAAKIAGVWLMPAD
                     ASKPSDARIKSGNYIKKKDKNV"
BASE COUNT          953 a          678 c          528 g         1233 t
ORIGIN      
        1 ggagaatcta acttagaaaa gctgaaaaat tgtcttgaca atggggggca ttatagtttg
       61 aatgtaatac ctttttcatt tttacaaaac aacttgccgt ttgaaatgct cataaaaatg
      121 gcaaggcgat catctgtgtt tgttttattg atcattatct caaaaatcgg ggttgctcca
      181 caaaaggaaa aaccatcaaa caaatatttt tcggcttgat ccgtatatcc gttttctaaa
      241 atataacttt tcaataaatt attgaattga ttttttttat cttttgaagc atcgcccttt
      301 ttacttacgg tcatcagcat atcaagttga gaatagtcca tacaaaaacc tccttaatgt
      361 tttggcacaa atttttattt tatactcttg aaccttattt gaacattcaa gtcaataaat
      421 acctggccat tttcattata caagtaacca taatcgtatc tgcttgcact gggcctgtca
      481 aatctggtgt ttttcaatag atattcttcg aaaaagttac gattatacaa atgatagcac
      541 aatacatcac catctctttt aataatgatg tatccgcccg tagcactgtc cagtccgtcc
      601 cattcctttc ccggggtcat tcccaaagcg gaagctgtaa ttaacttttt gattttatat
      661 ttatagacat ttggattatg ataatgcata ggattggctt cggttaagta tgtaaccaag
      721 gtatcacaat cgtagatacc gctttcaatt tcgctataat gttttaggat catttctccg
      781 tatatgcgag ggaggaggct atcaatcata attaaattat cattaaaagt ctgatctttg
      841 actttttcga aagatacatt ttcagcaaga gcaaaaagac gtgacattct cataaccata
      901 tagttacggt caacagtttc atcaatgtta ttgatttctt ctatttgcag gtcgttcaaa
      961 cccattactt tatattgaat gcgtgtattt tttcccgcat ttaacaaagt ggggggagaa
     1021 cccacatccg actttaccga aaatcccact tcgggactgt atcccgtata tatatcatga
     1081 atttgcatgg tcaggtcaat tttgtcggta gaaggagctt ttacttttct tatgtgcatt
     1141 cgttccataa acgaaaaagc ctcttcaata gtgaaagcac cgctgctttg agaatttctg
     1201 ctaccttcaa aaatcttttc aaacagagtt ctggtaggaa catccaattc attggccggt
     1261 atttcttcca aaaggcaatt gtttttgtaa attcttatta tatcgcctgt gtaataatcg
     1321 attttttcat tttttacttc ttctcttatt attttgatga taggtagata aatattgtcc
     1381 aatctgttga cattttcatc tgcagcataa attctgcctt ctttgagaag tttaatgaaa
     1441 gcgtagagtt ccgaccattc acctttattc tttgcattgg tttgcgttgt catgatcgag
     1501 aacctccttt atctttgttg caattgcttt tataaccggt accgtaacac tgtttcccaa
     1561 ttgtttatac aaatgtacat ctgccaaagg aagcttgaat gtatcgggaa atccttgtaa
     1621 tcttgcccat tcacgaggag tcattttgcg tataccttgg cgatttacct cgcccttgat
     1681 atgagtaaca ggcgtatagt cggtcaatct atcgtcaatg attaaattgc gttctttccc
     1741 cattccgcca catactattg caccggcaat tccattaagg tctcgaattt cgtaaccgaa
     1801 accgtttcct ttggattcat gccgtttttt atgatttatc aatgtattta aatattgagt
     1861 gcttaaatag tactttaccg aaaccggatt ttcttcaatt atatctttaa tgcaaacttt
     1921 cttgttaata ggagcgggga aagtaaaatt cgaagaatct atatcatttc taaatgcaac
     1981 tatgtaaata cgttctctgt tctggggtac accaaagtct ttactgttta atatttcgga
     2041 atatacggta tatcctaact ctcttaaagt ttccgtaatg attttaaatg ttcttccttt
     2101 atcatgtatg gtcaatcctt taacattttc gcagaatatt atcttaggtt tatggtaatc
     2161 gcaaattctg gctacatcga aaaacaatgt tcctcgtgac ttgcctttgt agtcatcatt
     2221 aaaacccata cgtttccctg ccaaactgaa tgcctgacat ggaaagcctg ccaaacaaat
     2281 atcaaactga gggatatctt tttcatctat ttgagtaata tctccggata tctctatttc
     2341 atcttcaaaa tttgatttat aagtcttttg tgcatattcg tcccattcac ttataaatac
     2401 ggttttgatt ctttcgccaa acgcttgatc aaaacccagt cgtataccac cgattccggc
     2461 aaagaggtca attgatctta gttgtttcat tttcttaccg ccttttctat ggcatccgca
     2521 caagatgcca gatttttttt gatatcgttt ccccaaaatc gcaatacagt ccatccttgg
     2581 aactctaatg tttgagtgac ttcaatatct cgttgcatgt ttctttcgat ttttgggatc
     2641 caaaagtctt gatttgattt aaaatcatgt tttctttctt cccaattata accatgccaa
     2701 aattcactgt cgcaaaagac ggctaccttt tttcctttaa atacgatatc aggtttccca
     2761 aaaatgtccg taacgttttt ccgatatctt aaaccgcgcg accacaactc ttttcttaac
     2821 attaattcga ttttggaatt tttgttcttt actcgactca tgttatattt gatttgttct
     2881 ctggtttttt tactcatata aaaatcactc cgaaaaagtg ttttatacat tcttatcctt
     2941 tttctttata taattcccgc tcttaattct tgcatcactg ggtttgcttg catccgcggg
     3001 cataagccaa acgcccgcta tttttgcagc gccttcgatt ctgccttctt cacaaagacg
     3061 cgttactctg cgagctgaaa ttccccattt ttcgcttgct tgcttggcgg ttaacaaatc
     3121 catgtaacca ctcctccaaa gttggaattt aaaatcagtt tttattatat tctaaattcg
     3181 gaataaagtc aaggtttgcc gagatatttc gacatttaaa ttaaaaaaag aaagtccgca
     3241 aacggacttt cgatctatta atcaatcttt gatatatata tataccctcc caatggattt
     3301 tgggtggtcg ggcgaacata aacaatgttc atatgctcat tataatgccc ccacttgtca
     3361 agacaatttt ttagcttttc taagttaggt tc
//
LOCUS       DAOSCH010000153         3383 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_17902_length_3383_cov_5.637019, whole genome shotgun sequence.
ACCESSION   DAOSCH010000153 DAOSCH010000000
VERSION     DAOSCH010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17902_length_3383_cov_5.637019"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PLZ54_08625"
     CDS             complement(<1..337)
                     /locus_tag="PLZ54_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67023.1"
                     /translation="MNNFITNSNKKQLKTRLTELLSKSEELKFLVGFFYFSGLRELYD
                     GLQSNDKVKLKVLIGLNVDKTIYGLTEYGENDIKLTDDERIENFFTSIRKSINSDVFD
                     HKDFYEQVKF"
     gene            1117..3327
                     /gene="ileS"
                     /locus_tag="PLZ54_08630"
                     /pseudo
     CDS             1117..3327
                     /gene="ileS"
                     /locus_tag="PLZ54_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
BASE COUNT         1099 a          599 c          681 g         1004 t
ORIGIN      
        1 aaaatttaac ctgctcataa aaatctttat ggtcaaaaac atctgaattt atggactttc
       61 ggatggaagt aaaaaagttt tctattcgtt cgtcatcagt aagttttatg tcattttcgc
      121 catattcggt cagtccgtaa atggttttgt ccacatttaa gccgatcagc acttttagtt
      181 tcactttatc gttcgattgc aagccatcgt acaattctct taaaccagaa aaataaaaaa
      241 atccgaccag aaatttcagc tcttcacttt tggatagaag ttcggtcaga cgtgttttta
      301 actgcttttt gttgctgttg gtaatgaaat tgttcataat tttttattga tcaataaaaa
      361 aattaaactg aaaaggaaaa ttctttttaa aatgattcgg caaaatatct tttaataaat
      421 tggcagctta ttgattaaaa agcaaagttt cacgcaaaaa aattgattaa gcagctgatg
      481 gtcagtggat gcatgctaat cccaaacact ttaaagatgc aacaaaccgc aaactcaatc
      541 aatataggag tttctgtagt aactattagt tatcccggct acgaagttaa ataaaagttt
      601 taaatcttac cttaatggag atattaattt tatctgccgt gatgtcttgg gtcgctgtat
      661 cttaatattt tggtcgggta cgggcaaaaa ttaagggatt tctttttcaa tataattttg
      721 taaaacaatg atatacaaag cctgctggta agccttcgga catttttccg atatattttt
      781 aacaaactgt tgatgttgaa aaaccgaatc ggaatcgggc gatgtagttt tcggagaaat
      841 aaatcaagga ggaaacacca aacaaggcaa cctttttttc ttcaaataat tattactctt
      901 tcagttccat aaccaatgcc cgaatacttc tttgtatccg acttttcgtt tttcaataaa
      961 aattagtaat tttgcacctt ccaaaaagta gcaaagaaag ccgaaaaaag caggaaggaa
     1021 tctcagaaat tgagttctat caaaaatttt tatgcaattc gggttttcag cgtttttgct
     1081 attggttaaa gacaggaatc agaggataaa cacgatatga aaaagtttgc agaatactct
     1141 tctttagatc tttcggaagt caacaaaaaa atgcttttac gttggaaaga aaaagacatt
     1201 ttccacaaaa gcattcaaat tcgagaagga gaaccaacgt ttgtatttta tgaaggcccg
     1261 ccttcggcca acggtatgcc gggcatccat catgttatgt cccgaaccat aaaagacctt
     1321 ttctgccgtt ataaaaccat gcagggtttt caggttaaaa gaaaagcagg atgggatacg
     1381 catggacttc cggttgagtt gagcgtggaa aaatcattgg gaatcactaa agaagatatt
     1441 ggtaagaaaa tcagtataga agaatacaat gcagcttgtc gtcgggatgt gatgaaatat
     1501 acccgcgagt gggaagattt gaccacaaaa atgggttatt ggattgattt ggatgatcct
     1561 tacattacct acgacaatcg ttatattgaa acactttggt ggttgctcaa acagctttat
     1621 gataaaaaat tgctttataa gggctatacc atacaaccct attcaccggc agcaggaacc
     1681 ggattgagca cgcacgaact gaatcagccg ggttgctatc gcgatgtaaa agatactacc
     1741 tgcgttgctc agttcaaagt gaaggatact tcttctttgc cattcaaaac taaaataaat
     1801 ggcgattgct attttctggc atggaccact acaccttgga ctttaccttc caatacagcc
     1861 ttgtgcgtag gaccaaatat tacgtatgtg cttgtagaaa cgttcaatcc ttataccggc
     1921 aaacccataa aagtaatttt ggccaaagat ttggtaaaca cttatttcaa ccaaaaaaac
     1981 gaaaatcttc cttttgaaaa ttatcagccg ggtgataaaa atatacctta ccgcattttg
     2041 gacgaatgca aaggcagcga attggtcggc attcgatacg aacagcttat tacgtgggtt
     2101 aatcccggag ataatgcttt tcgtgttatt gcgggagatt ttgtttctac ggaagaagga
     2161 accggaatcg ttcacattgc accaactttt ggggctgatg acgatagggt ggcaaaacaa
     2221 aataacatac cacccatgct tttgctcgac aaggacggga acaagcaacc aatggtggac
     2281 agaacgggaa aattttttaa attggaagac cttgattcag aatttgtttc taaaaatata
     2341 aatgtttcgc tttacagaga atacgccggc agatatgtaa agaatgaata tatttcagct
     2401 tttgaaccgg aaaatcagtc agataaaaat tcatctgtag aaaaaaccga agattcaacg
     2461 ctggatattg acttgtgcgt catgctcaaa caacagggat tggtattcaa aattgagaaa
     2521 tacacacaca gttatccgca ttgctggcga accgacaaac cggtgttata ttatccgctg
     2581 gacagctggt ttattaagac taccgcttta cgggatgaaa tgattgcatt gaaccaaacc
     2641 atcaattgga aacctgcttc tacaggatat ggacgttttg gaaaatggct cgaaaatctt
     2701 caggattgga atctttcacg aagtagattt tggggaactc cgcttcccat ttggcacact
     2761 gaagatggtt cggaagaaat ttgcattggg tcagtggatg aattgatttc agaaattgag
     2821 aaatccatac aagccggttt tatgacatcc aatccctata aaaatttcaa atccggcgat
     2881 tattcacgtg aaaattatgc tgttcaaaat atcgatttgc atcgtcctta tgtggacaat
     2941 attgtgctcg tatctaaatc gggaaaaccc atgagaagag agccggactt gatcgatgtt
     3001 tggttcgatt ccggttcgat gccatttgca cagttgcatt atcctttcga gaacagagaa
     3061 gccattgatc aacatacttt ttatccggcc gattttattt cggaaggggt ggatcagaca
     3121 cgtgggtggt tttttacttt acatgccatc agcacaatgg tgaaaggttc ggtagcctat
     3181 aaaaatgttg tttccaatgg cttggtgctc gacaaaaatg ggaataaaat gtcgaaacgt
     3241 ttgggcaacg tggtagatcc ttttgaaacc atcgaaaaat acggttctga ccccttgcgg
     3301 tggtatatga tgaccaattc agcaccctga gacaatttga aatttgatat tgaaggaggg
     3361 gaagaagtga gaaggaaatt ctt
//
LOCUS       DAOSCH010000154         3352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_18156_length_3352_cov_5.279648, whole genome shotgun sequence.
ACCESSION   DAOSCH010000154 DAOSCH010000000
VERSION     DAOSCH010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18156_length_3352_cov_5.279648"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PLZ54_08635"
     CDS             <1..332
                     /locus_tag="PLZ54_08635"
                     /inference="COORDINATES: protein motif:HMM:NF026165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOJ67024.1"
                     /translation="FLQKRTADDIWKNLYEFPLIETDHLLQVQELLENEFFCQLFQGL
                     EQVKIINISKPVHHLLTHRRISAQFITLQIAEKNEALHQFIEIPNAEIGDFAVSRLVE
                     MYLKKEI"
     gene            complement(342..1175)
                     /locus_tag="PLZ54_08640"
     CDS             complement(342..1175)
                     /locus_tag="PLZ54_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67025.1"
                     /translation="MKSIQLSTVFFLSIIFLVACNSRGKQEKEADNQLQKIQQLIQQN
                     ELNAAKIAIDSFHANFPRLVAKRRIAEAFQDTITRRECKRTIAYCDSILPFKQKEADS
                     LLLNFRLEKNEKYQDIGNYVYKTQSTEANAARTYLKAYVDENADYYLVSQYMGPKIEH
                     TSVEISNGEVFAHTDTIDITSASYLSFSDETGRWEIVTFKNEAENGVSEFISQYMNEN
                     LKVILHGKKNFSYFLQNNDKKAIKETYHLWIVKKDINRLKKEIEKAKLKIEQINRRNS
                     V"
     gene            2094..3320
                     /locus_tag="PLZ54_08645"
     CDS             2094..3320
                     /locus_tag="PLZ54_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOJ67026.1"
                     /translation="MSLNDQLHKQRSLFPEECLLKEIANQPRLSIGIPKENASIETRL
                     ALTPEGVAIVTEEGHSVYVERGAGEPMSYSDLQYSEAGAFLVDSPQDAFSADIVIKIT
                     PPTLDEINCMNDHASIFSMLQLSNLSTESIKAMMSKKLNAIAYELIRDEQKGFPVVSS
                     ISEIEGNTAITLIAELMSNQHGGKGLLLGSVAGISPPEVVILGASITGTVAARTALAL
                     GAQVKIFDHDINKLRTIQHHLGQQVFTSVIHPAVLFKALATADAVIGNLRYINGSERF
                     MVSEDLVKTMKRGAIIVDLSMDQGGCFETSECRTLQNPIYEKHGVIHYCIPNISARVA
                     RTSSMALSNILSPILLKIGHSGCINAAIAESSGFRHGVYVYKGFLVNRLIGDYYNIKS
                     NDINLLLYDMAQKIWR"
BASE COUNT         1062 a          596 c          613 g         1081 t
ORIGIN      
        1 tttttttgca gaaaagaaca gcggacgata tttggaaaaa tttgtacgaa tttccgttga
       61 tcgaaacaga ccatttgttg caggttcagg aattgcttga aaatgagttt ttttgccagt
      121 tgtttcaggg actggaacaa gtaaaaatta taaacatttc aaaacctgtt catcaccttt
      181 tgacgcatcg gcgcatttcg gctcagttca ttactttaca gattgccgaa aaaaatgagg
      241 cactgcatca atttattgaa attccaaacg cagaaattgg cgattttgct gtttcgcggc
      301 tggtagaaat gtatttgaaa aaagaaattt gatttttccg cctaaaccga attcctcctg
      361 ttaatttgct ctattttcag ttttgctttc tcaatttcct tttttaagcg gttaatatct
      421 ttttttacta tccataaatg ataagtttcc ttaatggctt ttttatcgtt gttctgaaga
      481 aagtagctga aattcttttt tccgtgtagg ataactttca gattttcatt catatattgc
      541 gaaataaatt ctgaaacccc attttcagct tcgtttttga aagttacaat ttcccaacgt
      601 ccggtttcat ccgaaaagct caaataagaa gcactggtaa tgtcgatggt gtcggtatgt
      661 gcaaaaactt cgccgttgga tatttcaacc gaagtatgct caatttttgg tcccatatac
      721 tgactcacca aataataatc ggcattttca tctacatatg ctttcaaata agtacgagct
      781 gcatttgctt cagtcgattg ggttttgtaa acgtaatttc caatgtcctg atatttttcg
      841 tttttttcca accgaaaatt tagcagcagt gaatcggctt ccttctgttt aaaaggcaaa
      901 atactgtcgc aatatgctat tgttcttttg cattctctgc gtgtaatagt atcctgaaaa
      961 gcttcggcta ttcgtctttt ggcaaccaaa cgcggaaaat ttgcgtgaaa actgtcgatg
     1021 gcaatttttg cagcatttaa ttcattttgc tgaatgagtt gttggatttt ctgaagttgg
     1081 ttatcggctt ctttttcttg cttccccctg ctattgcatg ctaccagaaa aattatcgac
     1141 aagaaaaata ctgttgagag ttgaatagat ttcatcagaa tataatttat tttttaaagg
     1201 tctgatgaaa cccacatgtc tgaatattat attcattcgt gtttgtgtat gcaatacatg
     1261 tgggtttcat aatgttattg gaaaattcaa ttgaaaatac aaaacataca aatgtataaa
     1321 acaaattgta cataaaaaag aaggtgtata ctttttaatg ttatcaaagt taaaatatcg
     1381 gtatctaaaa ggtaatttac tgccttccat taatgtttaa gaacgaaagg gttgacaatg
     1441 aggacatggt ttgtattcct aacagagttt attttgcatc gggtaatagt ttacaatgct
     1501 gcatttggaa taaattattc cgaaagcata tctttaatcc ttagaatgta caataaataa
     1561 ctttttgttg gagaattgtg aactgaattt aatgatattc ataagatttt taaatgttga
     1621 tttactcaaa tatattcagg acaaggcaac cccaagagct tgacttatat gtcctgtttt
     1681 acagatatat aaaacaaatg tctcaatttt tgtcatgtac taaaataaaa attttaaaaa
     1741 ctattttccg agaatatact gatttccgag aatatactga gattttcctc cgaatacaat
     1801 gagccggtga tactcacttc caaaagccct gaatggaaat tagccattcc caatgaactt
     1861 ttcaacgaaa aagctccctg tcgcattgtt ttgcatctgg caggtgaata tccgaaagat
     1921 aataccgaaa aaatattttt tgtggaaaat caattgacaa tcaaatattt aacaaaatat
     1981 caaacaaaaa taaaaaaatt aattcaacaa taatagcatt aatttagata caatttttgc
     2041 tcatttcaag aatagtattt agttttgtaa aatattgcaa aaacatactg aaaatgagtt
     2101 taaacgacca actgcataaa cagcgttctt tatttcccga agaatgtttg ttgaaggaaa
     2161 ttgccaatca accacgattg tccattggaa ttcccaaaga aaatgcctcc atcgaaacac
     2221 ggctggcact tactcccgaa ggcgttgcca ttgttacgga agaaggtcat agcgtttatg
     2281 tggagcgagg tgcaggcgaa cccatgtcct actccgatct acaatacagc gaagcaggag
     2341 cttttctggt cgattcgcca caagatgctt tcagtgctga tatcgtcata aaaattacac
     2401 cacccacatt ggatgaaata aattgcatga atgatcatgc atccatattt tccatgctgc
     2461 aacttagtaa tttatctacc gaaagcatta aggcaatgat gagcaaaaag ctcaatgcaa
     2521 ttgcttatga actgattcgc gacgaacaaa agggatttcc ggtggtcagt tcaatttcgg
     2581 aaattgaagg aaacacagcc atcacactta tagccgaact aatgagcaac caacatgggg
     2641 gaaaaggctt gttgctggga agtgttgccg gcatcagtcc tcctgaagtg gttattttgg
     2701 gggcaagcat caccggtacc gttgctgcac gaacagcttt ggcacttggg gcacaagtca
     2761 aaatttttga ccacgatatc aataaattgc gaaccatcca acatcatttg ggacaacaag
     2821 tttttacttc tgtcattcat ccggccgtac ttttcaaagc tcttgcaaca gctgatgcgg
     2881 taataggaaa tttgagatat atcaatggtt ccgaaagatt tatggtttcg gaagatttgg
     2941 taaaaacgat gaaacgtgga gcaattattg ttgacttaag catggatcag ggcggatgtt
     3001 ttgaaacttc cgaatgtcgt accctgcaga atccgattta cgagaagcat ggcgtaattc
     3061 attattgcat tcccaatatt tctgcccgag tggcccgaac atcctctatg gcgctcagca
     3121 atattctgtc tcccattttg ttgaaaattg gtcactccgg ctgtatcaat gctgccatag
     3181 cagaaagttc gggatttcgt catggcgttt atgtttataa gggattcctt gtaaacaggt
     3241 taattggcga ctactacaac ataaaatcga atgacataaa tttactgtta tatgacatgg
     3301 ctcaaaaaat atggaggtaa aaatgaaaaa aacaattgga acaattactg cg
//
LOCUS       DAOSCH010000155         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_18645_length_3286_cov_9.280409, whole genome shotgun sequence.
ACCESSION   DAOSCH010000155 DAOSCH010000000
VERSION     DAOSCH010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18645_length_3286_cov_9.280409"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            22..1197
                     /locus_tag="PLZ54_08650"
     CDS             22..1197
                     /locus_tag="PLZ54_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOJ67027.1"
                     /translation="MSEWKEYKFSDFVEINPNVILPSDESISYVEMKDLNESEKFCFP
                     SSERKKGNGSKFQNYDTLFARITPCLENGKICQVRGLKNNVGLGSTEFIVFRGKRNVS
                     DTDFIFYLSRWDDVRSHAEINLDGTSGRQRVPIACFDDLIIKMPVDIDEQKAIASILS
                     SLDDKVDLLHRQNATLEKIAETLFRQWFIEEAKEEWEIGKLDDVLSVIESGKRPKGGI
                     DPELTFGVPSIGAENINGIGVYDFSKTKFITEEFYHSMQRGIIKDYDVLIYKDGAYVG
                     RKGMFGNGFPFQEAAVNEHVFILRSNEKTNQFFLYFLLNQEELEQLNANSAQPGLNQE
                     AIKAFEIIIPPYGLMSKFGEFVKPFIDKIFFNGNQIRILTTLRDTLLPKLMNGEIKV"
     gene            1205..2182
                     /locus_tag="PLZ54_08655"
     CDS             1205..2182
                     /locus_tag="PLZ54_08655"
                     /inference="COORDINATES: protein motif:HMM:NF023776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3644 domain-containing protein"
                     /protein_id="HOJ67028.1"
                     /translation="MAKRIRKIWSVKNELLTKSREAMLCAVQIFNNPNIFFKSESFIV
                     LSNISWTYLLHAYYREKKVNYRYYEQKAKRKKYDTTKKGAFKYWELERCLKDENCPID
                     KITKLNLFFLIGLRHEIEHQMTSRIDDFLSARFQACCLNYNYYIKKLFGEEFGIDKHL
                     SFSLQFSSISDEHAEQLNEFSELPKNIASFINDFDNSVSDEEFNNPQYSYRILYVPKI
                     ANRKGQADKVIEFIPASSPEAQGLNKEYVLIKDREKPKFLPKQIIERMKALGYKKFGM
                     YQHTQLWKSLSAKEESKGFGVEVANTWYWYENWLQEVEKYCKQNQDTLK"
     gene            2182..>3286
                     /locus_tag="PLZ54_08660"
     CDS             2182..>3286
                     /locus_tag="PLZ54_08660"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOJ67029.1"
                     /translation="MTRITENSIEEFAIKLLEKLGYESVYGPDIAPDGDNSTGSMHDK
                     RESYEQVLLIDRLRKALKRINRNMPADTLEDAIKEIQRIHSPELLTNNETFHRFMTEG
                     IPVSKRVDGEDRGDLVWLIDFKNPYNNDFLVVNQFTVVENNHNRRPDIILFVNGIPLV
                     VIELKNAADENASIRSAFRQIETYKALIPQLFTYNGFVVISDGLEAKAGTISSGFSRF
                     MAWKSSDGKIEASHLVSQLETLIEGMLNKETLIDLMRHFIVFEKTKKEDPETGIITIN
                     TVKKMAAYHQYYAVNRAVESTLRAAGYTVSSRTSDTEVSRTSDTEVLRTSDTVTVPLP
                     EALEGSDKEALEDSEKEVLEDTVSFEASSVQETP"
BASE COUNT         1133 a          533 c          681 g          939 t
ORIGIN      
        1 cccatctaaa aatgaatacc aatgagtgag tggaaagaat ataaattttc agattttgtc
       61 gaaatcaatc ctaatgtaat tttgccttca gatgaaagta tttcgtatgt tgaaatgaaa
      121 gatttaaatg aaagcgaaaa attttgtttt ccgagttctg aaagaaaaaa agggaatggt
      181 tcaaaattcc aaaattatga tactcttttt gccagaataa caccttgtct tgagaatgga
      241 aaaatttgtc aagtcagagg attaaaaaat aatgttgggt taggttctac ggagtttatc
      301 gtttttagag ggaaaagaaa tgtttccgat acagatttca ttttttattt atcgagatgg
      361 gatgatgtac gatcacatgc agagattaat ttggatggca catccggaag acagagagtt
      421 ccaatagcat gtttcgatga tttaattatt aaaatgccag tggatataga tgagcaaaaa
      481 gccatagcct ccattctttc cagcctcgat gataaagtag acctgcttca ccgccaaaac
      541 gctacgctcg aaaaaatagc cgaaacactt ttcaggcagt ggtttattga ggaagcaaaa
      601 gaggagtggg aaattgggaa attagatgat gtattatcag tgatagaatc tgggaaaagg
      661 ccgaaaggag gtattgatcc ggaattaaca tttggggtcc caagtattgg agctgaaaac
      721 attaatggaa ttggtgtata cgatttttca aaaacaaaat ttatcactga ggaattttat
      781 cattcaatgc agagaggaat aataaaagat tacgatgtat taatttacaa ggatggcgca
      841 tatgttggta gaaaaggaat gtttggaaat gggttccctt ttcaagaagc tgctgtgaat
      901 gagcatgttt tcattttgag aagtaatgag aaaactaatc aattttttct ttatttttta
      961 ttgaatcagg aagagttaga acaattgaat gctaattcag cacagccagg attaaatcaa
     1021 gaagcaataa aagcttttga aataattatt cctccgtatg gattgatgtc gaagtttggt
     1081 gaatttgtga agcctttcat tgataaaata ttttttaatg gcaatcaaat tcgaatcctc
     1141 accactcttc gtgacacctt gttgccgaaa ttgatgaatg gagaaataaa agtgtaatta
     1201 taaaatggcg aaacgtatca ggaaaatatg gagtgtaaaa aatgaactct tgacgaaatc
     1261 aagagaagcc atgctctgtg cagtgcaaat ttttaacaat ccgaatatct ttttcaaatc
     1321 cgaaagcttc atagtgctat ctaatatttc ttggacatat ttacttcatg cttattacag
     1381 agaaaaaaaa gtaaattata gatattatga gcaaaaggca aagcgaaaaa aatatgatac
     1441 gaccaaaaaa ggggccttta aatattggga gctcgaaaga tgtctaaaag atgaaaattg
     1501 cccaattgat aaaataacca aattaaatct attcttttta attggactta gacatgagat
     1561 agagcatcaa atgacttcaa gaattgatga ttttttaagt gcaagatttc aagcttgctg
     1621 tttgaattat aactactaca taaaaaaact ttttggagag gaatttggta ttgataagca
     1681 tttatccttt tctctccaat tttcttccat tagtgatgaa catgcggagc agcttaatga
     1741 attttctgag ttgcccaaaa atattgcatc tttcatcaat gattttgaca attctgtttc
     1801 tgatgaagag tttaataatc cgcaatattc atatcgtatt ctttatgttc ccaaaatagc
     1861 caatagaaaa gggcaagcag ataaagtaat tgaatttatt cctgcaagtt cacctgaagc
     1921 acaaggtctc aataaggaat atgttcttat aaaagatagg gaaaaaccca aattcttgcc
     1981 aaaacaaata attgaaagga tgaaagctct aggttacaaa aagtttggga tgtatcaaca
     2041 tactcaatta tggaaaagtc taagcgccaa agaagaaagt aaaggttttg gagttgaagt
     2101 ggctaatact tggtattggt atgagaactg gcttcaagaa gtagagaagt actgtaaaca
     2161 aaatcaagat actctgaagt aatgacacgc attaccgaaa atagcataga agaatttgct
     2221 atcaagttac tggaaaaact tggttacgaa tcggtttatg gtcccgatat tgcccctgac
     2281 ggcgacaatt cgacaggctc aatgcacgac aaacgggaaa gctacgaaca ggttttattg
     2341 atcgatcgtt tacgcaaagc actaaaacgt atcaacagaa acatgcccgc cgatacgctg
     2401 gaagatgcca taaaagaaat ccaacgcatt cactcaccgg aattgttgac gaataacgaa
     2461 acttttcacc ggtttatgac tgaaggtatt cctgtttcaa agcgggtgga tggcgaggat
     2521 cggggcgacc tggtttggct gattgatttt aaaaatccgt ataataacga ttttttggtt
     2581 gttaaccagt ttaccgttgt ggaaaacaac cataacagac ggcccgatat cattttgttt
     2641 gttaacggca ttccgttggt agtcatcgag ctgaaaaatg ctgctgatga aaatgcgtcc
     2701 attcgttcgg catttcgaca aatagaaact tataaagcgt tgatccccca acttttcacg
     2761 tataatggtt ttgtggttat ctccgatggg ttggaagcca aagcgggaac catttcttcg
     2821 ggattcagcc gttttatggc ctggaaatca tccgacggga aaattgaagc ctcgcatttg
     2881 gtcagtcagt tggaaactct tattgagggt atgctcaata aggagacgtt gatcgacttg
     2941 atgcgtcatt tcattgtttt tgaaaaaacg aaaaaagaag atcccgaaac gggaattatt
     3001 accataaata ccgttaaaaa gatggctgcc taccatcaat actatgcggt gaaccgcgct
     3061 gttgaatcga cattgagagc agcgggatat acggtgtctt cgagaacctc agacaccgaa
     3121 gtttcgagaa cctcagacac cgaagtattg agaacctcag acaccgttac agtaccgctg
     3181 cctgaggctc tcgaaggcag cgacaaagaa gcactcgaag acagcgaaaa agaggtactc
     3241 gaagacaccg tttctttcga agccagcagt gtccaagaaa ctcccg
//
LOCUS       DAOSCH010000156         3277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_18718_length_3277_cov_7.179392, whole genome shotgun sequence.
ACCESSION   DAOSCH010000156 DAOSCH010000000
VERSION     DAOSCH010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18718_length_3277_cov_7.179392"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            368..670
                     /locus_tag="PLZ54_08665"
     CDS             368..670
                     /locus_tag="PLZ54_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67030.1"
                     /translation="MPTILDLVKVPYPATYQGNKIQPLEGVSLRQAFLTGKQDIQGYQ
                     FWEHSNKRAVTKGKWKAVSKIGTDNWELYDILTRTSQNQTSFSKLDKNISFCNVCQ"
     gene            1361..2353
                     /locus_tag="PLZ54_08670"
     CDS             1361..2353
                     /locus_tag="PLZ54_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67031.1"
                     /translation="MKKLTLLFVVVWAMMLVFTFSSNAQTVFALQNGSRTQFYTTLST
                     AISNIQDGDTLYLPGKPLDGDYTFSKKVTIIGAGYDPDSAAATGITKITGKVYFAKNS
                     KGSSMTGVRTEGRVYVRDSSITITRCNLESLEIQNGSNPIGFVYVGDCIIRNNIEGAN
                     EVVTNVLIERCILNSNFGGGNKTNNLLIKNCVLTYNGNYFLRELSNIIFQNSIFLSTN
                     SSFIVGSSSLTFINNLFVRPAFSNITFVMTGNIFEVPESDIFVDSANGNYHIKPTCTQ
                     ALTLATDGKEVGIYGTDNPFLVPTFAPRFISINNAESTKDGKLSVKMTVEARNR"
     gene            2447..>3277
                     /locus_tag="PLZ54_08675"
     CDS             2447..>3277
                     /locus_tag="PLZ54_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67032.1"
                     /translation="MKNTIRIIVILVILLAISPPFFSQNSIKTVEFWTDGNSANCTRQ
                     TISASSVFLWQDEINLASLVDGLHTFNVRFKDEYGVWSPIETRFFVKQTNALGQGELK
                     KITALEYWMNGNSSNRTQRILNGQTTFAWEELIDVAGLSDGVNTFNARFKDEYGVWSP
                     IETRFFVKQTNALGQGELKKITALEYWMNGNSSNRTQRILNGQTTFAWEELIDVAGLS
                     DGVNTFNARFKDEYGVWSPVETRFFLKQTNAAGQGVLKKIVALEYWMNGNSANRTQRT
                     L"
BASE COUNT         1134 a          586 c          561 g          996 t
ORIGIN      
        1 tagagcgcga cactggcagt gtcgaggtaa ccggttcaag tccggtaatc tccactttta
       61 aacagagttt tttttcttct tcttcttctt cttcttcttt tattattatt ccttccataa
      121 ttattatctt ttcaatattc tttacaccac taatcttttc acatatttta acaactcttt
      181 actaaacttt cttaaaaaac aattcaaacg atcataagaa aaaatttaaa ctattaaaaa
      241 aatattaaaa tcaatttgtt acgttttaag agaaaaaatt tacattttga gaactttttt
      301 ctttgttaga agttatattt gtatattaaa taaaattatt aatatctgcg ggcatattct
      361 aaatattatg ccaaccattc ttgatctcgt taaagttcca tatccagcta catatcaagg
      421 taataaaatt caaccgcttg aaggtgtgag tcttagacag gcatttttaa caggtaaaca
      481 agatatacaa ggataccagt tttgggaaca ttcgaataag agagcagtta caaaaggtaa
      541 atggaaagca gtttctaaaa ttggaacgga taactgggag ctttatgata tcttaacccg
      601 gacaagccag aaccaaacaa gtttttcaaa gttggataaa aatatttcct tctgcaatgt
      661 ttgtcaataa ggcgttacag aagattgtca aaaatattat gccaaaaaat acatcaattt
      721 agaagataag gtattaaaaa ggcttcattt aaagattatt cggatcagga catcaaaaat
      781 attcagtaca aactaaaccg aagacctcga gaaaaattag attttaaatt accccaaaat
      841 gagtttttta aacatttttt taatttcgca cttgctggtt gaatctacat agttaaaaag
      901 ggaaccattc ttaaagacaa ttacgcaaat aaagaagaaa tgaatgaggc tttgatggct
      961 tttcccgtat attacatgct ttatagaaga catggcgaag tgaaaaaaaa ctgggcgtaa
     1021 aaaccccttt tcaggccgtc gaaaaatggg cttatgttaa aacttgaaaa ttttttaaaa
     1081 aaatccttgc atttttaaaa ataaagttgt atatttacaa caaaataaaa gaccaagttt
     1141 taataaacaa ccttgtgaaa cttgacaggt acagacctac aaatttattg ctttcagctt
     1201 tttcaggaat attatataac tgaaaaatca atatacaaac ttatcactag gaataaaaga
     1261 aatcatttaa aaatcaattt tatccaaaat ctctaatctt tatcattcta caaaaaattt
     1321 tccaaaaaca aattcattca ttttaataaa aaattatctt atgaaaaagt taactttact
     1381 ctttgttgtc gtttgggcta tgatgctcgt attcacattt tcttccaatg cccaaaccgt
     1441 ttttgcgctt caaaacggaa gcagaaccca gttttatact actttgtcta ctgccatcag
     1501 caacattcag gatggagata cactctattt gccgggaaag ccattggatg gagattatac
     1561 tttttctaaa aaagtaacca ttatcggcgc cggctatgat cctgatagcg cagctgctac
     1621 cggaataaca aaaattaccg gcaaagtata ttttgccaaa aacagcaaag gctcttccat
     1681 gactggagtt cgcactgaag gacgcgttta tgtacgggat tcctcgataa ccattacccg
     1741 atgcaatctg gaatcattag aaattcaaaa tggaagcaat ccgattggat ttgtatatgt
     1801 gggcgattgc attatccgca acaacattga aggagcgaac gaggtggtta ccaatgtgct
     1861 gatagaacgt tgcatactca acagtaattt cgggggtgga aataaaacca ataatttgtt
     1921 gataaaaaat tgtgtcttaa cttataacgg taattatttt ttaagagaac tcagcaatat
     1981 aatatttcaa aattcgatat ttttaagcac caatagcagt tttatagttg gtagctcttc
     2041 ccttacgttt attaacaatt tatttgttcg ccctgctttt tctaatatca ctttcgttat
     2101 gacggggaat atatttgagg tgcctgaaag tgatatattt gtagattcag caaatggaaa
     2161 ctatcacatt aaacctactt gcacacaagc attgacactt gctaccgatg gtaaggaagt
     2221 cgggatatat ggcacggaca acccttttct tgttccaaca tttgcaccgc gatttatttc
     2281 cattaacaat gcggaaagca cgaaagatgg aaaattgtct gtaaaaatga cggtagaagc
     2341 cagaaataga taaagcctca atcgccaaag tgtcaggaat tatccgacat taatattcgt
     2401 ttgaaaaata atcaattgat aagatttttc aaaactaatc acgaaaatga aaaatacaat
     2461 aagaattata gtaatacttg tcatacttct tgcaatttca cctccatttt tttcacaaaa
     2521 ttcaattaaa actgtagaat tttggacgga tggaaacagt gcaaattgca cccgtcaaac
     2581 gatttcggca tcaagtgttt ttttatggca agatgaaatc aatcttgctt cgcttgtgga
     2641 tggtttgcac actttcaatg tccgtttcaa ggacgaatac ggcgtttggt cgcccattga
     2701 aacccgattc tttgtcaaac aaaccaatgc actgggacag ggagaactga aaaaaattac
     2761 tgctctcgaa tactggatga atggaaattc ttccaacaga acgcagcgga tattgaacgg
     2821 acaaaccaca tttgcatggg aagaactaat tgatgtcgcc ggactttcgg atggcgtcaa
     2881 taccttcaat gcacgtttca aggacgaata cggcgtttgg tcgcccattg aaacccgatt
     2941 ctttgtcaaa caaaccaatg cactgggaca gggagaactg aaaaaaatta ctgctctcga
     3001 atactggatg aatggaaatt cttccaacag aacgcagcgg atattgaacg gacaaaccac
     3061 atttgcatgg gaagaactaa ttgatgtcgc cggactttcg gatggcgtca ataccttcaa
     3121 tgcacgtttc aaggacgaat acggcgtttg gtcgcccgtt gaaacccgat tttttctcaa
     3181 gcaaaccaat gctgccggac agggcgtttt gaaaaaaatt gtcgctcttg aatattggat
     3241 gaatggaaat tctgccaaca gaacacagcg aaccttg
//
LOCUS       DAOSCH010000157         3182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_19499_length_3182_cov_7.263192, whole genome shotgun sequence.
ACCESSION   DAOSCH010000157 DAOSCH010000000
VERSION     DAOSCH010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19499_length_3182_cov_7.263192"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..485
                     /locus_tag="PLZ54_08680"
     CDS             195..485
                     /locus_tag="PLZ54_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HOJ67033.1"
                     /translation="MINFNGLLVGFAVFLLIGIFHPIVIKAEYFWGVRSWWIFFIIGS
                     IAAVVSLFVEKEIPSILLGVFAFSCFWSIREIFQQKRRVEKGWFPKNPKRKK"
     gene            624..1334
                     /gene="pyrH"
                     /locus_tag="PLZ54_08685"
     CDS             624..1334
                     /gene="pyrH"
                     /locus_tag="PLZ54_08685"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005784714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOJ67034.1"
                     /translation="MLYYRRILLKLSGESLMGGKQYGLDENRLQEYAAQIAEVAKMGV
                     QVGIVIGGGNIFRGLSGTKKGFDRILGDQMGMLATVINSLALHSALQAEGATSHVLTA
                     IRMEPVGEYYSKQKAVSSLEKGEIVIISGGTGNPFFTTDTASSLRAIEIGADIMLKGT
                     RVDGVYTADPEKNSDAERFEEITYDEVYRRNLKIMDLTATTLCKENNMPIYVFNMDTI
                     GNLKKVILGEKIGTLIKN"
     gene            1445..2005
                     /gene="frr"
                     /locus_tag="PLZ54_08690"
     CDS             1445..2005
                     /gene="frr"
                     /locus_tag="PLZ54_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOJ67035.1"
                     /translation="MQDVKPILDEAKESMETTIQFLENALAHIRAGKANPRILDAVRV
                     DYYGVHVPLANVASITTPDAKTILIQPWEKAMLKPIEKAILNSDLGINPTDNGEVIRL
                     SMPPLTEERRKQLVKQVRMEVEEARIRIRNARRNAIDHIKKMIKEGLPEDVEKDAEEQ
                     VQKLHDDYIKIIDEMLALKEKEIMTV"
     gene            2153..3091
                     /gene="rsgA"
                     /locus_tag="PLZ54_08695"
     CDS             2153..3091
                     /gene="rsgA"
                     /locus_tag="PLZ54_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOJ67036.1"
                     /translation="MLGTVVKSTGSWYLVETEEKERVECKIKGNFRLKGIRSTNPIAV
                     GDRVEFEKNEDGIGLINRIYDRKNYIIRRSSNLSKEFSIMAANLDIAALIITLNYPET
                     STVFIDRFLAVTEAYNVPACLIINKTDLYGKEDVEYMEALAYLYENIGYPVFKISVLK
                     GENLSAIKEFFTGKTVLLAGNSGVGKSSLLNALSPEARAKTADISFYHNKGMHTTTYS
                     EMYRLPDGGYIIDTPGIKGFGLIDMEESEVDHYFKEIFKISSQCKYDNCLHIQEPECA
                     VREAVEKGEISWSRYQSYLSVLEDCHAEKYRKDEYH"
BASE COUNT         1082 a          509 c          636 g          955 t
ORIGIN      
        1 agggcattct aaaatcggga tgcccttttt aattctatgt aagtgtttta aacacatatc
       61 agatttgttg aagttgatcc aacaaaattt tcaaaattac tgaatgcgaa aactatttgc
      121 cgaattctcg acaaatagtt ttttaatttt ataaaattat tttcgatctt tgtatcaaaa
      181 aaacaactca tcttatgata aatttcaatg gtttgttggt tggctttgca gtttttcttc
      241 tgataggaat ctttcaccca attgttataa aagccgagta tttttggggt gtccgaagct
      301 ggtggatatt ttttataatt ggttcaattg ccgccgttgt ttctttgttt gtggaaaaag
      361 aaataccctc aattttgttg ggagtttttg cattttcttg tttctggagc atacgcgaaa
      421 tttttcagca gaaacggagg gtggaaaaag gttggtttcc caaaaacccc aaaaggaaaa
      481 aataatttta aattttgctg tttttgtgtt tgtcataagt ttgtaaatac tatttattta
      541 tttatttttg tgtggttatc tgtagtttgt tctgcataac aacgaaaaaa caaaaatttc
      601 ttgaacctta aaaacatatc aaaatgttgt actacaggcg gatattgtta aaactgagtg
      661 gagaatcttt aatgggagga aaacaatacg gacttgacga aaaccggctg caggaatatg
      721 cggctcaaat tgcagaagtt gcaaaaatgg gagttcaagt tgggatagtg attggtggag
      781 gaaatatttt tcggggtttg agtggaacaa aaaaaggctt tgaccgcatt ttaggcgacc
      841 aaatgggaat gcttgccacc gttatcaaca gtttggctct tcattcggcc ttgcaggcag
      901 aaggagcgac ttcacacgtg ctcaccgcca tacgcatgga accggtagga gaatattaca
      961 gcaaacaaaa agccgtttca tctttagaaa aaggtgaaat cgtcattatt tccgggggaa
     1021 cgggaaatcc gttttttacc accgataccg cttcatcact tcgtgccata gaaatcggtg
     1081 ccgatatcat gctgaaagga acccgagtgg atggcgttta tactgccgat cccgaaaaaa
     1141 acagcgatgc agagagattt gaagaaataa cttatgatga agtttaccgg agaaatctga
     1201 aaataatgga tttaaccgcc acaaccctat gcaaggaaaa caatatgcct atttatgtat
     1261 tcaacatgga tacaattgga aatcttaaaa aagttatttt gggagaaaaa attggcactt
     1321 taataaaaaa ttgataccat aaagaaaaat caatttaaat tgaattgaat tgaattggtt
     1381 tgaaaatcct tatcgaaaat aaataatcac ggaagaagaa tcttaaaaac taaattttat
     1441 ggacatacaa gatgtaaaac caattttgga tgaagcaaaa gaaagtatgg aaacaaccat
     1501 acagttttta gaaaatgcat tggcacatat tcgtgcagga aaggctaatc ctcgaattct
     1561 ggatgcagtt cgtgtggatt attatggcgt tcatgttcct cttgccaatg ttgcttccat
     1621 tacaactccc gatgcaaaaa caatccttat tcaaccttgg gaaaaggcaa tgctcaaacc
     1681 cattgaaaaa gctattttaa attctgattt gggaattaat cccaccgata atggtgaagt
     1741 cattcggctt tccatgccac cactgactga agaaagacgc aaacagctgg ttaaacaggt
     1801 tagaatggaa gtcgaagaag cccgtattcg gataagaaat gctcgcagaa atgccattga
     1861 ccatatcaaa aaaatgatta aggaagggct tccagaagac gttgaaaaag atgcagaaga
     1921 acaagttcaa aaacttcatg atgattacat taaaatcatt gatgaaatgc ttgctctcaa
     1981 ggaaaaggag attatgaccg tttaacaagg gtattttcaa tggtaagaat tgataaaact
     2041 tgctttcgat ataacctcgg atcaagtttt atcaatcttt ttatgaaatt tcaatgccgt
     2101 aattccaaat ctcaaaaaaa ggttgttaaa ataaatttga agtgaacatg taatgttggg
     2161 caccgtcgta aaaagcaccg gcagttggta tctggttgaa actgaagaaa aagaacgggt
     2221 agaatgtaaa attaaaggga attttcgttt aaaagggatt cgaagtacca atcctattgc
     2281 tgtgggcgac cgtgtggaat ttgaaaaaaa cgaagatgga ataggcctga ttaacagaat
     2341 atacgacaga aaaaattata ttattcgtcg ctcgtccaat ttatccaaag aattcagcat
     2401 catggccgca aatctcgata tagcggctct tatcattacg ttaaattatc ccgaaacttc
     2461 taccgttttt attgaccgtt ttttggctgt aaccgaagcg tataatgttc ctgcttgtct
     2521 gataatcaac aaaactgatc tctatggtaa ggaagatgtt gagtatatgg aagcacttgc
     2581 ttatctttat gaaaacattg ggtatcccgt ttttaaaata tcggttttaa aaggtgaaaa
     2641 tctttcggca ataaaagaat tttttaccgg taaaacagta cttttggcag gcaattcggg
     2701 agttggaaaa tcttcattgc tcaatgccct atcgcctgaa gctcgtgcca aaacagccga
     2761 tatttctttt tatcataaca aggggatgca tactaccact tattcggaaa tgtatcgatt
     2821 acctgatgga ggttatatta ttgacacacc gggtatcaag ggatttggct tgatcgacat
     2881 ggaagaatcg gaagtagatc attattttaa ggagattttt aaaatttcct cacaatgcaa
     2941 gtacgataac tgtctgcata tacaggaacc ggaatgcgct gtaagagaag ctgttgaaaa
     3001 gggggaaatc agctggtcgc gctatcaaag ctatttaagt gttttggaag attgccacgc
     3061 cgaaaaatac aggaaagatg aatatcatta atttatgcaa attattctca ataaaactat
     3121 tttaaaagct gaattcaagt cacaaatgca actttttggt tatacaaatt tcgtaataaa
     3181 aa
//
LOCUS       DAOSCH010000158         3180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_19520_length_3180_cov_7.779520, whole genome shotgun sequence.
ACCESSION   DAOSCH010000158 DAOSCH010000000
VERSION     DAOSCH010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19520_length_3180_cov_7.779520"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLZ54_08700"
     CDS             complement(<1..373)
                     /locus_tag="PLZ54_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HOJ67037.1"
                     /translation="MGNLNFKVQSLFCEQVSEWELAKKNYEDLKKVEIKVFSFGDFEI
                     VVQFNPARIVSSGAKVDEKTIKERKCFLCRQNLPPQQKCVDAEDYTVLVNPYPIFPEH
                     FTIPRKEHIPQQIKPFFPDMLE"
     gene            complement(425..1537)
                     /locus_tag="PLZ54_08705"
     CDS             complement(425..1537)
                     /locus_tag="PLZ54_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ67038.1"
                     /translation="MLKYLLEKEFKQIMRNKFLPRMVIILPFVALALFPLVANFDVKN
                     INLAVVDNNHSQYSERLIQKVISSGHFILTNVSHNYSDALKSVEQNKSDVILEIPAQF
                     EKDLVNNLTASVLISANTVNGMKGGLSSAYLVGIITDFSSEIREEWIQSGTKLKVPAC
                     EIIPQYRFNPHLEYKYNMIPAIMMMMLAMITGFLPALNIVGEKEKGTIEQMNVTPVNK
                     FTLILSKLIPYWVIGFIVLTICFFVAWLFYGMISKGGYLQIYLFAAVFVLAFSGLGLV
                     ISNYATTIQQGMFMMFFFVITFIFLSGLYTPISSMPEWTQTMSFFSPLRYMIEALRSI
                     YLKGSSFMDLWHQFMALVGFAIFFNFWAINSYRKKA"
     gene            complement(1555..2661)
                     /locus_tag="PLZ54_08710"
     CDS             complement(1555..2661)
                     /locus_tag="PLZ54_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004300010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ67039.1"
                     /translation="MKQFMSFVRKEFYHIFRDKTTMAILLILPILMLVLFGYSISTEV
                     KNSKIAILDSSNDVATRAIIEKFRNSEYFSVKKMVNNPSEIQDLFKKDRLNMVLVFGD
                     NFYENLAHTGNAQVQLIVDGSDPNSAKTIVTYATSIIGAYQLELSKIDKIPFQINPQI
                     KLLYNPQMKGAYNFVPGVMGMILMLICAMMTSISIAREKELGTMEVLLVSPMKPIYII
                     LSKIIPYFVLSLVNLATILLLSVFLLKVPIAGSLFWLIAFSLVYIFVNLSLGLLISSI
                     VDSQLVALLISGMVLMLPVIMLSGMMFPVENMPVFFQWISQIIPAKWYIIGVKKIMIK
                     GLGLSSIIPEMSVLLGMGIVLVIASLKNFKYRLE"
     gene            complement(2663..>3180)
                     /locus_tag="PLZ54_08715"
     CDS             complement(2663..>3180)
                     /locus_tag="PLZ54_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008660546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ67040.1"
                     /translation="KRNIGYMSQKFALYEDLKVWENIRLYGGIYGVPEDEIIKKTDEL
                     LKRLGFEKERNSFVKSLPLGWKQKLAFSVAIFHEPKIVFLDEPTGGVDPVTRRQFWEM
                     IYEAADRGITVFVTTHYMDEAEYCDRVSIMVDGRIDALDSPKNLKNQFKANNMDGVFQ
                     QLARRASRKAD"
BASE COUNT         1017 a          652 c          561 g          950 t
ORIGIN      
        1 attcgagcat atcggggaaa aaaggtttta tttgttgcgg aatatgttct ttgcggggaa
       61 tggtaaaatg ctcgggaaaa atagggtagg ggtttaccaa aacagtataa tcctctgcat
      121 cgacacattt ttgttgtggt ggcagattct gacgacacaa aaaacatttc ctttccttta
      181 tggtcttttc atctactttt gcacccgacg aaacaatgcg tgccgggtta aactgcacta
      241 caatttcaaa atcgccaaaa gaaaaaacct taatttcaac ttttttcaaa tcttcgtaat
      301 tctttttggc caactcccat tcggaaactt gttcacaaaa caagctctgc accttaaaat
      361 tcaaatttcc catcaaaaat tattctgtct aaaaatttat ttcctcaaaa attgaaattg
      421 gaccttaagc tttttttctg taactattga tagcccaaaa attgaaaaaa atagcaaaac
      481 cgaccaaagc cataaattga tgccacaaat ccataaaact gctgcctttg agataaatgg
      541 aacgcagagc ttcaatcatg taacggagcg gactgaaaaa gctcatcgtc tgagtccact
      601 ccggcatact ggaaataggc gtgtaaagcc cactcagaaa tataaatgtg atgacaaaaa
      661 agaacatcat aaacattccc tgctgaatag tggtagcata gttggagata accaacccca
      721 gacctgagaa agccaataca aaaaccgctg cgaaaagata aatttgcaga taacctcctt
      781 ttgaaatcat tccgtaaaac agccatgcca caaaaaaaca tattgtaaga acgataaaac
      841 caatgaccca atacggaata agttttgaaa gaatgagcgt gaacttattt accggagtta
      901 cattcatctg ctcaatagtt cctttttctt tttcccctac aatattcaat gccggtaaaa
      961 atccggttat catggccagc atcatcatca taatggccgg aatcatgttg tacttatact
     1021 ccagatgagg attaaatcgg tattgcggaa taatttcgca ggctggaact tttagttttg
     1081 taccactctg aatccattct tctctgattt cactgctgaa atcggttatt attccgacta
     1141 aataggcact gcttagtcca cctttcattc cgtttacggt gtttgctgaa atcagcacac
     1201 tggcagtaag gttatttact aagtcttttt cgaattgagc cggtatttcg agtataacat
     1261 cagatttgtt ctgttctaca cttttcagtg catctgagta attatgcgaa acatttgtca
     1321 ggataaaatg ccccgacgaa atgaccttct gaatcagacg ttcggagtac tgactatggt
     1381 tgttgtcaac cacagcaaga ttaatgtttt taacatcgaa gttggctacc aatgggaata
     1441 gcgcgagtgc aacaaacggc aagatgatga ccatacgggg caaaaacttg ttgcgcataa
     1501 tttgcttgaa ttctttttct aataaatatt tcagcataat tttgtctcct gttattattc
     1561 caacctatac ttgaaatttt tcagacttgc tatcaccaac acgattccca ttcctaataa
     1621 aacactcatt tccggaatga tggaagataa accaagtcct ttaatcatga tttttttgac
     1681 ccctataata taccatttcg cggggataat ttgcgaaatc cattgaaaga aaaccggcat
     1741 attttcgacc ggaaacatca ttcctgatag cataatgacc ggtaacataa gcaccattcc
     1801 cgagataagc agagccacca gttgcgagtc tactatcgaa gaaatcaaca accccaacga
     1861 tagatttaca aaaatataga ccagcgagaa tgcaatcaac cagaacaagc tccctgcaat
     1921 tggaaccttg agcaggaaga cggataagag taaaatggta gctaaattta ccagcgacaa
     1981 aacgaaatag ggaatgattt tggaaagtat gatatagata ggtttcattg gtgatacaag
     2041 caaaacttcc attgtgccaa gttctttttc gcgggcaatg gagatggaag tcatcatggc
     2101 acaaatcagc ataagtatca tacccattac acccggaaca aaattgtaag cacctttcat
     2161 ttgtgggttg tagagcaatt ttatttgtgg atttatttga aacggaatct tatcaatttt
     2221 agatagttcc agttggtaag ctccaataat actggttgca taagtcacaa ttgttttggc
     2281 cgaattagga tcactgccat caacaattag ttgtacttgt gcgttgcccg tatgtgccag
     2341 attttcataa aagttatcac cgaaaacgag taccatattc agtctgtctt ttttgaataa
     2401 atcctgaatt tcggacggat tatttaccat tttcttgact gaaaaatact cgctgttcct
     2461 gaatttttca ataatagcgc gtgttgccac atcatttgaa gaatctagta tggcaatttt
     2521 agaattcttg acttcggtgc tgattgaata tccaaacaac acaagcatca gaatgggtaa
     2581 aatcaacaaa atagccattg ttgttttatc cctgaaaata tggtaaaatt cctttcgaac
     2641 gaaggacatg aattgtttca tactaatctg cctttcttga agcgcgtcgc gcaagttgtt
     2701 gaaaaactcc atccatattg ttggctttaa actggttttt tagatttttt ggagaatcga
     2761 gagcgtcaat tcgtccatca accataatgg aaacccgatc gcaatattct gcttcgtcca
     2821 tataatgcgt ggtaacaaaa acggtgattc cgcggtcggc ggcttcgtaa atcatttccc
     2881 aaaactgacg acgcgttacg ggatccactc cgcctgttgg ctcatcaaga aaaactatct
     2941 taggctcatg aaaaatggca accgaaaagg caagtttctg cttccatccc aaaggaagcg
     3001 atttcacaaa cgaatttctt tctttttcaa atccaagtcg ttttaataac tcatctgttt
     3061 tttttataat ttcatcttca ggcacgccat aaattccacc atacaaacgg atattttccc
     3121 aaaccttcaa atcctcgtac aaagcaaatt tttggctcat gtagccgatg tttcttttca
//
LOCUS       DAOSCH010000159         3112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_20106_length_3112_cov_8.666012, whole genome shotgun sequence.
ACCESSION   DAOSCH010000159 DAOSCH010000000
VERSION     DAOSCH010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20106_length_3112_cov_8.666012"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..187)
                     /locus_tag="PLZ54_08720"
     CDS             complement(47..187)
                     /locus_tag="PLZ54_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67041.1"
                     /translation="MPNPIHGIIIINKNNAAKQINKLQPSKNQLIWQRNDAEHIIRND
                     MN"
     gene            complement(231..>3112)
                     /locus_tag="PLZ54_08725"
     CDS             complement(231..>3112)
                     /locus_tag="PLZ54_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOJ67042.1"
                     /translation="DEAESYFDELWNNAIKITENDELKEKIVTVVEHETLMKKITPFE
                     AYLFVLKNYLDTFRGKEITQRLTEVLEENGYKAYRYQLDAVQQALSITENNNGVIIAD
                     VVGLGKTIIACSIAFELKKRGIVIAPPGIIGDKAKSEGWKKYLEQFHLASLGWEAFSL
                     GDLEHVSEFVSKTNDIEVVIIDEAHRFRNEDTKDYEYLKNICRNKIVILLTATPFNNK
                     PADIFSLLKLFIVPKKSSITLDDDLENKFRTFKSEFDKLSYIKRYHQSSDRQKQNRAL
                     SYYNDLFGDQKIVDSTKIKKRTEILAKQIRNVIEPVIIRRNRLDIQNHPYYKNEVNEL
                     SIVESPKEWFYELNKEQSVFYDKIIQQYFALPDDGGRFKGAIYKPFEYELQKVKKGEL
                     GKEENFQYLQQFNLYDFMRRLLVKRFESSFGAFEQSVKNFINIHQIVLEFIQKTDKYI
                     LDRNLLEKIYELDADEIENYLQEYSEKIKNNVYPKNHKVYELKKFERQTEFIQDIQSD
                     IALFQQILNELHKLNLVAKDPKAECLIEEVKTILDAPPENLHEPKRKVIIFSEYTDTV
                     NYLAPLLTQVFNARVLVVAGDLSASKIKEINKNFDASATQQADDYDILLTTDKISEGF
                     NLNRAGMVINYDVPWNPVRVIQRVGRINRISRKVFDKLYIINFFPTEKGSDLVHSREI
                     AAQKMFMIHNTLGEDAKIFDIDEEPTPSGLYNKILQSPDAIEGESSYTKISKEFEAWK
                     EKYPDMVASLKNFPSRIKVAKDGAENELFVVIKKQKLFVHQQLYDAKEGSENHFIKEV
                     EDILEKIKADINEPPLPLSNQFWEEYEKIKNYQENKKNNKPLPDNNLQTRAINVLRNF
                     LKINDNQSVTILKPFVRMLLEDLLDYGTLSDYTIRRIANIDIDKNFTKAVDELKSIEK
                     ELGSDYLYKEKQRISDVSKEIIIAIENKKTNELPTQNTFNDNISK"
BASE COUNT          840 a          543 c          569 g         1160 t
ORIGIN      
        1 ttttcccatt tcaaaggatt gtcaaaaata tattggcgaa tgcgatttaa ttcatgtcgt
       61 tgcgaataat gtgttcggca tcattccgtt gccaaatcaa ttgatttttt gatggttgca
      121 atttattgat ttgttttgcg gcattgtttt tattgataat gatgatgccg tgaatagggt
      181 ttggcattat aatccattta ttgaattgaa cgaaaaattc cattcaatat ttattttgaa
      241 atgttgtcat tgaatgtatt ttgtgttggc aattcattcg tttttttgtt ttcgatggca
      301 ataataattt ctttcgagac atcggaaata cgttgttttt ctttgtataa ataatcgctc
      361 cccaattctt tttcgatgga tttcaattca tcaactgctt ttgtgaaatt tttatcaatg
      421 tcaatattgg caattcttct gatggtgtaa tcggaaagcg taccgtaatc gagcaaatct
      481 tccagcagca ttcttacaaa aggtttcagt atggtaaccg attgattatc atttattttt
      541 aagaaattac gcaacacatt aatagcacgc gtttgcaggt tgttatcagg gagcggttta
      601 ttattttttt tattttcctg ataattttta atcttttcgt attcttccca gaattggtta
      661 ctcaagggta aaggaggttc gtttatgtcg gcttttattt tttcgagaat atcttccact
      721 tctttaataa aatgattttc acttccttcc tttgcatcat agagctgttg atgaacaaaa
      781 agcttttgtt tttttataac cacaaacaat tcattttctg caccatcttt ggcaactttt
      841 atgcgcgaag gaaaattttt cagcgaagcc accatgtcag gatatttttc tttccatgct
      901 tcaaattctt tcgatatttt cgtataggaa ctttcgcctt cgattgcatc tggactttgc
      961 agaattttgt tataaagtcc tgatggcgta ggttcttcgt cgatatcgaa aattttggca
     1021 tcttcgccca gcgtattgtg aatcatgaac attttttgtg ccgcaatttc tcttgaatga
     1081 accagatccg aacctttttc ggtagggaaa aagttgataa tgtaaagttt atcaaaaact
     1141 tttctgctga tgcggttgat tcttcctaca cgctgaatca cgcgtaccgg attccaggga
     1201 acatcgtaat tgattaccat gccggcacga ttcaaattga atccttccga aattttatcg
     1261 gtagtcaata agatatcata atcgtcagcc tgttgagtag cggaggcatc gaaattttta
     1321 ttgatttctt tgattttact tgccgacaaa tctccggcaa ccaccagtac acgtgcgttg
     1381 aaaacttggg tgagcaaggg agccagataa ttgaccgtat cggtatattc cgaaaaaatg
     1441 ataacttttc ttttcggttc atggaggttt tcgggcggag cgtcgagtat tgtttttact
     1501 tcttcgatga ggcattcggc tttggggtct tttgctacca gattcagttt gtgcaattcg
     1561 ttaagaattt gctggaataa tgcaatatct gattgaatat cctgaataaa ttcggtttga
     1621 cgttcaaatt ttttcagttc atacacttta tggtttttag gatatacatt atttttgatt
     1681 ttttcagaat attcttgcaa ataattttca atttcgtcgg catccaattc ataaattttt
     1741 tccagcaaat tcctgtcgag aatatattta tcggtttttt ggataaattc cagtacaatt
     1801 tgatggatgt tgatgaagtt tttcacgctt tgttcaaaag cgccaaatga actttcgaaa
     1861 cgttttacca gcaatcgccg cataaaatcg tacaagttga attgttgcaa atattggaaa
     1921 ttttcttctt tgcccagctc accttttttt actttttgca actcatattc aaaaggtttg
     1981 taaatggctc ctttaaaacg tcctccgtca tcaggcaagg caaagtattg ttgaatgatt
     2041 ttgtcgtaaa ataccgattg ttccttgttc agttcataga accattcttt tggagattct
     2101 acaattgata gctcgtttac ttcatttttg taataaggat gattttgaat gtccagccga
     2161 tttcggcgaa taatcactgg ttcaataaca ttgcgaattt gttttgccag aatttctgtt
     2221 cgttttttta ttttagtcga atcgactatc ttttggtcgc caaataaatc attataataa
     2281 gaaagggctc tgttttgctt ttgccggtca gacgattggt gatatcgctt gatgtaggaa
     2341 agtttatcaa attccgattt gaatgttcta aatttgtttt ccaaatcatc atcaagggta
     2401 atacttgatt ttttgggaac aataaagagt ttgagcagcg aaaatatatc ggctggtttg
     2461 ttgttgaaag gagtagcagt gagcaagata acgattttgt tgcgacaaat atttttcagg
     2521 tactcgtaat ctttggtatc ttcatttctg aatcgatgcg cttcgtcaat aattaccact
     2581 tcgatatcgt tggttttcga aacaaattcc gaaacgtgtt cgaggtcgcc aagtgaaaaa
     2641 gcttcccagc caagagaagc cagatgaaat tgttcaagat attttttcca gccttccgat
     2701 ttggctttat cgcctatgat gcccggaggt gcaattacaa ttccacgctt tttgagttca
     2761 aaagcaatac tgcaagcaat aatggttttg cccaatccca ctacatcggc aatgattacg
     2821 ccgttgttgt tttcagtaat ggaaagtgct tgttgaacgg catctaactg gtaacgatag
     2881 gctttgtatc cattttcttc aagcacttcg gtaagccgct gcgtaatttc ttttccacga
     2941 aaggtgtcaa gataattttt caaaacaaaa agataagctt caaaaggcgt aatttttttc
     3001 atcagcgttt catgttcaac aactgtgaca attttttcct tcaactcgtc gttttctgta
     3061 atttttatgg cattattcca gagttcatca aaataacttt ctgcttcatc aa
//
LOCUS       DAOSCH010000160         3105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_20171_length_3105_cov_5.265902, whole genome shotgun sequence.
ACCESSION   DAOSCH010000160 DAOSCH010000000
VERSION     DAOSCH010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20171_length_3105_cov_5.265902"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..2972
                     /locus_tag="PLZ54_08730"
     CDS             168..2972
                     /locus_tag="PLZ54_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOJ67043.1"
                     /translation="MKKSIHLFVLSFIFLICCPFAEAQSPKREMRAVWLATVWQLDWP
                     SITVPKATGSNESARQAAINQQKNELTVIFDSLKKANMNTVFFQVRGMCDAMYTSSYE
                     PWSAYISSERGANPGYDPLAFAIEEAHKRGLELHAWLNPYRYSSSVYSHGNTSIDYSI
                     THPEWLLDYGNYVKILNPGLPEVRERIKCIIGEIVNNYNVDGIVFDDYFYAYGGTPNN
                     LDAAAQALYKPANQTIGDWRRENINKMIAGVYDTIQKVKPYVTFGVSPFGTWTTDQNV
                     AAQRGIPLPIGVGTTGNMYAEIYCDPVAWLEQGTVDYLSPQLYWTTYSAYPYGILAEW
                     WSKLTNRFGKHFYSSHSLSSMTASSAPAAVPSKIKINNEEIPTSAFSTLELELYGAKK
                     STSVSAAPSAVNFLPSEIGLQIDFNRDYDINDAPGSVFYATSKFMNTSGFISYLRNQK
                     FIHPALCPAISWKTHTDLPLISNLTISGNTLSWTYENDNVCFAVYAVPNENRNLPGIF
                     TSSDYLLGVSYNKQFILPASVNENSHKIAVTVMDRFGNEGSPRIYGESLTPSVAAQLI
                     YPENNTETVIPCVFQWETVQEADSYIWQLAEDENFTNLVCSRQTKENSFFSGKQTNLK
                     DSTKYYWRVKTLKANAEEIWSEIRNFTSHKFKILSPTNGSTNVSLLPAFKWDSISPTA
                     SYTLEISSVWNFLESKRVFITTVNANSFVVPYGTLVGGTKYYARVTVNDGDISAVTES
                     ISFSTVEETIPVPQILLPTNGSTIQGNSIQVCWAEQPARGFRVELSTSSAFPPRSTTV
                     KTVDAYTFCTTYEQLTNNTYYIRVKASTPNGLTEASDTVAVTLKDNTSIGNNALMVNW
                     KGYFVKNGNNIHLMLDVPSTEKVSIKLYSITGTLLFQKEITLQEGINQIPISTEHLLQ
                     GIYPLEITGEKNRLILKLIY"
BASE COUNT          999 a          611 c          590 g          905 t
ORIGIN      
        1 cagggttaat gcgatacaat ccggcaaaag gatatgttga acctgtcatt gaaggtgtgg
       61 aaatttacgg agtagccata aataaaacac catcaaaact cttttaatct gggaatttaa
      121 aagaaatttt gaaagttgag aataacttta aaaaaagaaa tataggcatg aaaaaaagca
      181 ttcatctatt tgttttatct ttcatttttt tgatttgttg tcctttcgcc gaagcgcaat
      241 cgcccaaacg cgagatgcgg gctgtatggc tggctacggt atggcaactc gattggccaa
      301 gcattacggt tcccaaagca acgggaagta atgaatcggc acggcaagcc gccatcaatc
      361 agcaaaaaaa tgaattgaca gtgattttcg attcactgaa gaaagcaaat atgaataccg
      421 ttttttttca agttagagga atgtgcgacg ccatgtatac ttcttcctac gaaccctgga
      481 gtgcttatat ttcgagcgaa cgcggagcca atcccggtta tgatccattg gcctttgcca
      541 ttgaagaagc ccataaacgc ggtttggaac tccatgcgtg gttaaatcct tatcgatatt
      601 cttcttcggt atattctcat ggcaatactt ccatcgatta ttccattact catcccgaat
      661 ggttactcga ctacggaaat tatgttaaaa ttctaaatcc cggattaccc gaagtaaggg
      721 aaagaataaa atgcatcata ggtgaaattg ttaataacta taatgtagat ggaatagttt
      781 ttgatgacta tttctatgct tatggaggta cacctaacaa tttggatgca gctgcgcaag
      841 ctttatataa gcctgcaaat cagactattg gcgattggcg aagagaaaat ataaacaaaa
      901 tgattgccgg agtttacgat actattcaaa aggtgaaacc ttatgttacc tttggagttt
      961 caccttttgg aacttggact accgaccaaa atgtggcagc tcaaagagga attcctctgc
     1021 caatcggagt cggaacgaca gggaatatgt atgccgaaat atattgcgat cccgtagcat
     1081 ggctcgaaca gggaacagta gattatctct ctccccaact ttattggacc acttacagtg
     1141 cctatcctta tggaattttg gccgaatggt ggtctaaact gaccaatcgc ttcggtaaac
     1201 atttttattc cagtcattca ctttcttcga tgactgcttc gtcggcgcct gcagccgtac
     1261 caagcaaaat taaaataaac aacgaagaaa ttccaacctc agctttttcc actctcgaat
     1321 tggaattgta tggggctaaa aaatcaacgt ctgttagcgc agctccaagt gcggtcaatt
     1381 ttctgccttc cgaaataggc cttcaaattg atttcaacag agattatgat atcaacgatg
     1441 ctcccggaag cgttttttat gctaccagca aattcatgaa tacctcagga tttatatctt
     1501 atttgagaaa tcaaaaattt attcatcctg ctttatgtcc cgctatcagt tggaaaacac
     1561 atactgatct tcctttgata agcaatttga caatttcggg aaatactttg tcgtggactt
     1621 atgaaaatga taatgtctgt tttgccgttt acgcagtacc caacgaaaac agaaatttac
     1681 ccggaatttt cacttcctct gattacttac ttggtgtgag ttacaacaaa caattcattt
     1741 taccggcaag tgtaaatgaa aactcccata aaattgctgt aacggtgatg gacagatttg
     1801 gaaacgaagg ttcacccaga atttatggag aatcgcttac gccttctgtt gcagctcaac
     1861 ttatttatcc ggaaaataac actgaaacag tcattccttg tgtatttcaa tgggaaacgg
     1921 ttcaggaagc tgacagttac atctggcaac tggcagaaga tgaaaatttt acgaatttgg
     1981 tttgttcgag gcaaacaaaa gaaaatagtt tcttctcagg aaaacaaaca aatttaaaag
     2041 actccacaaa atactactgg cgagtgaaaa ccttaaaggc caatgcagaa gaaatctggt
     2101 ctgaaattcg aaatttcacc agtcataagt ttaaaatact ttctccaaca aacggaagca
     2161 caaatgtttc acttttgcct gcttttaaat gggactccat tagcccaacc gcttcctata
     2221 cacttgaaat ttcttccgta tggaattttt tggaatccaa acgggtattt atcacaaccg
     2281 taaatgccaa cagctttgtt gttccttacg ggacgttggt tggtggaaca aaatattatg
     2341 cacgggtaac ggtaaacgat ggggatattt cggctgtaac ggaatctata agtttttcta
     2401 ctgtagaaga aaccattcca gtacctcaga tattgctacc aaccaatggc agtaccattc
     2461 agggaaattc catacaagtt tgctgggctg aacaacctgc cagaggattc agagtagaat
     2521 tatctacctc atcggcattt cctccacgca gcacaaccgt aaaaaccgtt gatgcctata
     2581 ctttttgtac aacttatgaa caactgacca ataacacata ttatatccgt gtgaaagctt
     2641 ccaccccgaa tgggttgacc gaagcttcgg atactgttgc ggtaacactg aaagacaata
     2701 catcgatcgg caacaatgcc cttatggtaa attggaaagg atatttcgtg aaaaatggca
     2761 acaacattca tttaatgctg gatgtacctt ctacagaaaa agtttcgata aaattgtatt
     2821 ccatcacggg aactttactc ttccaaaagg aaataacttt gcaggaagga attaaccaaa
     2881 ttccaatatc tacagagcac ttgctgcaag gtatttatcc attggaaata accggagaaa
     2941 aaaacagact gatactgaaa ctgatttatt agcttttgac aaaaaaggga atttctaatt
     3001 atttattaat tttatattta ctaactttta aattttatta gattatgaaa aaacaagtac
     3061 tttttggaat agcattgcta tgcagtgcat tcctgggcaa tgtaa
//
LOCUS       DAOSCH010000161         3067 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_20517_length_3067_cov_6.254980, whole genome shotgun sequence.
ACCESSION   DAOSCH010000161 DAOSCH010000000
VERSION     DAOSCH010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20517_length_3067_cov_6.254980"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..2609)
                     /locus_tag="PLZ54_08735"
     CDS             complement(252..2609)
                     /locus_tag="PLZ54_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOJ67044.1"
                     /translation="MSRIKLRTWLYYVLMLLLFCVGAYLLIQKGSDVEANYHLTTQSV
                     STQVLTPEPNLSNWEQFKVSVSHNMGEPITHLLLQIIAILLVSRLFGLFFSKINQPTV
                     IAEILAGIVLGPSLLGHFFPNIFNFLFAADSLPNLYILSQIGLILFMFVIGMELNMKA
                     LKEKASQVLVISHASILIPYFLGMSLAYVYYEEFAVNQTKFLPFALFIGISMSITAFP
                     VLARIVQEKGLTKTHLGIIAIASAAIDDVTAWCLLALVIAISKTGDSVSALYTVGLSV
                     AYILLMFFVIRPFLKKIGEIYQNIETLNKSIVAFLFLVLISSAFITQFIGIHALFGAF
                     LAGVVMPPIPNFRKLLIEKIEDVSLTLLLPLFFVFTGLRTEIGLLNSPYLWGICGIFI
                     ATAIVGKFLGGAFSARFLGESWHDSLALGTLMNTRGLMELIVLNIGYEMHILPPPIFV
                     MLIIMAIVTTFMTTPILNLIEKVFPVKNVEEEYIRKQSMGIFKVLIAVGNPENGRMLL
                     RVAKNVLEGVKNTLSVTVLHITPGTDTNPIYGDQFAEESFHKVKQEAEQLNIPIETEY
                     KVVDSIENAIVRMANTNNFDFLLVGAGLSLSGIPFFKERAVFKNIGWLNRLINNISKQ
                     QVVFYPGTLIKDKTKYFIEQSNCTVGVFVNRGFSEISSAAVLLYSEEDEFLLHYARRL
                     LRNNNNASVTIFDLNRVVASSEKVARYVEDLEKQFPASVKLNKQNKINSNSFSKFSLM
                     FISYQTWNALLEKDKSYLSNIPSTLIINKKNSRFHSDGRRRQVLS"
BASE COUNT         1042 a          605 c          538 g          882 t
ORIGIN      
        1 ccgccgaccc tctgcttgta aggcagatgc tctgaaccag ctgagctaaa cgcccttgtt
       61 tgtttatgta cctgctttcg gtaaaagcgt tgcaaagata cattgttttt tcaaattgac
      121 aaaaaatttg tttaattttt cgcatataaa atttttagat gctgtaaatt ggttcttatt
      181 aataatctca tcatttaaaa tcctgaaaaa attaataaaa ctgtctattc atctacattc
      241 tcttctgaaa tttaagataa tacctggcgc cgtctgccat cagaatgaaa acggctgttt
      301 ttcttgttga taataagtgt cgaaggtata ttcgacagat acgatttatc cttttccaac
      361 aatgcgttcc atgtctgata gctgataaac atcagactaa atttggagaa actgttggaa
      421 ttgattttat tttgcttgtt gagcttgaca gaagcgggga attgcttttc caaatcttca
      481 acataccggg ctactttctc gctggaagcc accaccctgt tcaaatcgaa aatggtaacc
      541 gaggcattgt tgttgttgcg caagagacgt cgtgcataat gtagcagaaa ctcgtcttcc
      601 tcactgtaga gcaaaacagc agctgagcta atttccgaaa atccacgatt aacaaacact
      661 cccaccgtac aattactctg ttctatgaaa tacttggtct tgtccttaat caaagttccc
      721 ggataaaaca ccacctgttg ttttgaaata ttattgatca gtctgttcag ccatcctata
      781 tttttaaaaa cagctctttc cttaaagaat ggaatacccg acaaggaaag tccggctccc
      841 accaaaagga aatcgaaatt gttggtattt gccattcgta caatggcatt ttctatgctg
      901 tcaaccactt tatattccgt ttcgataggg atattcagtt gctcggcttc ctgttttacc
      961 ttatggaaac tttcctctgc aaactgatct ccataaatgg gattggtatc ggtgccgggc
     1021 gtaatatgaa gtacagtaac ggaaagagta ttttttactc cttccaaaac atttttggca
     1081 acacgtaaca acattctccc attttccgga ttccctacag caatcaacac cttgaaaata
     1141 cccatcgact gcttgcgaat gtattcttct tcgacatttt tgacggggaa aactttttca
     1201 atcaaattca gaatcggagt ggtcataaag gttgttacta tggccatgat tatcagcatt
     1261 acaaagatgg gaggaggaag aatgtgcatt tcgtaaccga tattcaacac aatcaattcc
     1321 atcaacccac gcgtattcat aagtgttccc aatgccaagc tgtcatgcca cgactcgccc
     1381 agaaaacgtg ctgaaaaagc accgccaaga aattttccga caatagctgt ggcgataaaa
     1441 ataccacaaa ttccccataa ataaggagaa ttgagcaaac ctatttcggt acgcaagccg
     1501 gtgaatacga aaaacagagg caaaagcaaa gtcaacgaaa catcttcaat tttttcgatc
     1561 agcaattttc tgaaattagg aatgggaggc attactacgc cggccagaaa agcaccgaac
     1621 aaagcgtgga taccgataaa ttgtgtaata aaggcagaag aaatcaaaac cagaaaaaga
     1681 aaagcaacaa tacttttatt gagggtttca atattttgat aaatttcgcc aatttttttc
     1741 aaaaaaggtc tgataacgaa aaacataagt aaaatatacg caacagacaa tccaaccgta
     1801 taaagtgcgc taacggaatc gccggttttt gaaatagcaa tgaccagtgc gagtaaacac
     1861 catgctgtaa catcatcaat ggctgcactg gctatggcaa ttattcccaa atgtgtcttg
     1921 gtaagaccct tttcctgaac aatacgcgcc aaaacaggga aggcggtgat actcatggaa
     1981 attcctataa acagcgcaaa aggaagaaat ttagtctgat taacggcaaa ctcttcataa
     2041 taaacatagg ccaacgacat tcccaaaaaa taaggaatga gaatactggc atgactaata
     2101 accagcactt gacttgcctt ttctttcaac gctttcatat tcaactccat accaataaca
     2161 aacataaaaa gaatcaaccc tatctgactt agaatataca aattgggtaa agaatcggca
     2221 gcaaagagaa aattgaaaat attgggaaag aaatgcccca acaaagaagg tccgagcaca
     2281 atgccggcta agatttctgc tattacagtt ggttgattta tcttggaaaa aaatagtcca
     2341 aaaagcctcg aaaccagaag tatggcaatg atttgaagca ataaatgggt gatgggctcg
     2401 cccatattgt gggacactga aactttaaac tgttcccagt tgctgagatt gggttccgga
     2461 gtaagaactt gggtagagac actttgtgtg gttagatgat aatttgcctc cacgtcgctc
     2521 cctttttgta tcaacaaata agctccaaca caaaaaagaa gtaacatgag tacataataa
     2581 agccatgttc gcaatttgat tctggacata gaatttactt tatcatgcta tgcagtgaaa
     2641 attcattttt taaattctct gacagcctcc cacagttagg aaaagtaagg aaatccgcta
     2701 caaaaataca aaaaattaaa aaaacaatgc aaaactttct gctaaatcag cacctttatg
     2761 ttgaacttag catagtgtta gctatcgtaa aagataaatc ggttgtatgg cgtgaactat
     2821 atgtattaca ttaaattatt gtacaaataa attattatct ttgtccttag tagatgataa
     2881 aaaaaattgt aaaaacattt atgtttttta ttttcagata tttaataacg aagctttgcc
     2941 ccaaacctca cttacttttt tgtcttgaaa caaaaacgta agcaaaaaaa ttaacttagt
     3001 tgaagcagcc atgctcaaca taaatcaaat aaatttgatt ttgttttcgc ttaaacgctg
     3061 ctttcaa
//
LOCUS       DAOSCH010000162         3063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_20554_length_3063_cov_6.184840, whole genome shotgun sequence.
ACCESSION   DAOSCH010000162 DAOSCH010000000
VERSION     DAOSCH010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3063
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20554_length_3063_cov_6.184840"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..2668)
                     /locus_tag="PLZ54_08740"
     CDS             complement(197..2668)
                     /locus_tag="PLZ54_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67045.1"
                     /translation="MFYCIDFNLLVLWKMKYKDIREEELKNKVAYDYFWLYDCTKIIG
                     NVDFCVSMYQKAPSLFEQEFLLWAEAKRGSSDIYNSITQLILTIGKARTFDKYLPPAF
                     LGAFDGEKIAFIPYNEIHDIFYINDFNWNVTPSNYDTKEFKIIHDKVKAIIDSKTLLF
                     YFIDDDRELQKFIKNNFVVSKYGITKTRIDKNNFIVIYNKWLQTVKPTIAVNWEAAKK
                     NGIIDGDFYLADLLSQENNTLKEKLFVLLRHDHYELDRKIDEAGMFSSKRTDFNDNQV
                     AHTLFWNKYERPPKEEYWDYIVERRDLLVPQDVRERKGSFFTPQIWVELSQKYLTDVL
                     GENWQDDYYVWDCAAGTGNLLHGLTNKYNVWASTLDLQDVEVMHDRINNGANLLHDHV
                     FQFDFLNDEFLPQSQGGKLPDNLYKIISDAEERKKLVVYINPPYAEATTARTVTGTGE
                     NKAGVAIENIANRLFKRKIGNASNEIFALFMAQVYDKIPNCIHAQFSTLKFVQGSNFG
                     KFKEFFKAEYLGGFVVPANTFDNVKGEFPIGFTIWNLSKKDNIKQITCDVYNKNAEYI
                     GNKNFYGDLPPSINKWIAKFQLEDDNLLGLMVSAAPDFQHNNQLAILAKQQERYCFNI
                     TKKNLFVSAIYFSVRLCNERSWLNDRDQFLYPNNGWQTDTEFQNDCLVFTLFHGQNRI
                     SSKEGLNHWIPFAEDEVNARERFDSHFMNDFLKGKIKVANTGLFDVQPQRDTPLEFSP
                     EAHAVFDAGREIWKYYHSKPNVNVNASLYDIKEYFQGRNEKGKMNNKSTDETYTSLMV
                     NLRDKLKQLAEKIEPKIYEYGFLKL"
BASE COUNT          951 a          585 c          525 g         1002 t
ORIGIN      
        1 cacaatcaaa tcgtatcgtt tgggcttttt acaaccgctt ataacaattg caactgttag
       61 gaaaggtaga agaaatgtaa atcgattcat aagaattcat gttaggttgt tttcaaatat
      121 tttataaagt gcacctcaac ataggacatc aaactgccaa ttaaagtgat taattcgtaa
      181 aacaaataac taattattac aacttcaaaa atccatattc gtagattttg ggttcaattt
      241 tttccgcaag ctgttttaac ttatccctca aattgaccat aagggaagta taggtttcgt
      301 cggtactttt gttattcatt ttgccttttt cgttgcgccc ctgaaaatac tctttaatat
      361 cgtataacga tgcattaaca ttcacattgg gtttgctatg gtaatacttc caaatctcac
      421 gacctgcatc aaaaacggca tgagcttcag gcgaaaactc tagcggggta tccctttgcg
      481 gctgtacatc gaacaaacca gtatttgcaa ctttaatctt tccttttaaa aagtcgttca
      541 taaaatggct atcgaatcgc tcgcgggcgt ttacctcatc ctcggcaaaa ggtatccaat
      601 ggtttaaacc ctccttgctc gaaattcgat tttgcccatg aaaaagggta aataccaaac
      661 aatcattctg aaattcggtg tcggtttgcc acccgttgtt gggatataaa aattgatcgc
      721 ggtcgtttaa ccaagaacgt tcattacata agcgaactga aaagtaaatt gcagaaacaa
      781 ataaattctt tttagtaata ttaaaacaat acctttcttg ttgtttggct aaaattgcca
      841 attgattatt gtgctgaaaa tcaggtgctg cacttaccat taaacccaat aaattatcgt
      901 cttctaattg aaactttgca atccatttat ttatgcttgg gggtaaatca ccataaaaat
      961 tcttatttcc aatgtattca gcatttttat tatatacatc gcaggttatt tgctttatat
     1021 tgtccttttt acttaaattc caaatggtaa aaccaatggg gaattcacct ttcacgttat
     1081 cgaaagtatt ggcaggtacc acaaaaccac ctaaatattc ggctttaaaa aattctttaa
     1141 acttaccaaa gtttgaaccc tgaacaaatt ttaatgttga aaattgagca tgaatgcaat
     1201 tgggtatttt gtcgtaaact tgtgccataa acagagcaaa aatttcgttt gatgcattgc
     1261 caatttttct tttaaaaagc cggtttgcaa tattttcaat ggctactccc gctttgtttt
     1321 cacccgtacc ggtaactgtt cttgctgttg tggcttccgc atacggtgga tttatataaa
     1381 caactaactt ttttcgttct tctgcatcgg aaataatttt gtaaagattg tcaggtaatt
     1441 tgccaccctg cgattggggc agaaattcat cgttaaggaa atcgaactga aaaacatggt
     1501 cgtgcagcaa gttggctccg ttatttatgc gatcgtgcat aacttcaaca tcttgcaggt
     1561 cgagggttga agcccaaacg ttgtatttat tggtaagtcc gtgaagtaag ttacccgttc
     1621 ctgcagcgca atcccaaacg tagtagtcgt cctgccagtt ttcacctaag acatcggtta
     1681 ggtacttttg cgataattcc acccaaattt gcggggtaaa aaaacttcct ttacgctccc
     1741 ttacatcttg cggaacgagt aaatcgcggc gttcaacaat atagtcccag tattcctcct
     1801 ttggtggacg ctcgtatttg ttccagaata gcgtatgcgc aacctgattg tcgttaaaat
     1861 cggttcgttt gctactaaac atacctgcct cgtcaatttt ccggtcgagc tcgtaatggt
     1921 cgtgtctgag aagaacaaat aacttttcct taagagtgtt attctcttgc gagagcagat
     1981 cggcaaggta aaaatcgcca tcaataattc cattcttttt ggcggcctcc cagttaactg
     2041 caatagtagg ctttaccgtt tgcagccatt tgttgtaaat aacaataaag ttgtttttat
     2101 caattcgggt tttggtaatt ccgtatttgc ttacaacaaa attgttttta atgaatttct
     2161 ggagttctcg atcatcgtct ataaagtaaa acagcaaagt cttactatca ataatagctt
     2221 ttactttgtc atgaatgatt ttaaattcct tagtatcgta atttgaaggg gttacgttcc
     2281 agttaaagtc gttgatatag aaaatatcgt gaatttcgtt gtaagggata aacgcaattt
     2341 tttcaccatc gaaagcacca aggaaagccg gtggtaagta tttatcaaaa gttctagcct
     2401 taccaatggt aaggataagt tgagttatgg aattataaat atccgatgag cctctcttgg
     2461 cctcagccca aagtaaaaac tcttgttcaa aaagcgatgg agccttttgg tacatgctta
     2521 cgcaaaaatc aacattacca ataatttttg tgcagtcgta aagccaaaag taatcgtagg
     2581 caactttgtt tttgagttct tcctctctaa tgtccttata tttcattttc caaagtacta
     2641 ataaattaaa atcaatgcaa taaaacatta atatcgctaa tcgtttttgc tattaatgat
     2701 tgccattaca aacataatca atggaataaa tgattattag ggaaaggcga tgcttcgaca
     2761 aaactcagcg tgaaaagtat ttcggcagag ttctgaaaat tagcatcccc aaaaacttaa
     2821 ctaattgttt aacatcaaaa gaaattttat aattaaggca acgaaacata ataaatggca
     2881 ataatttatc tttcacctcg taaatttagt aactaacaag caaagaaaaa aattaggggt
     2941 tgcatttgca acccctattc atattctact taaatccccg atttcgtaaa agcggatcaa
     3001 ttgatggtgc tttaccacgg aatttcagat actgaaccat accatcgttg ttaccaccct
     3061 ctg
//
LOCUS       DAOSCH010000163         3015 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_20979_length_3015_cov_5.638176, whole genome shotgun sequence.
ACCESSION   DAOSCH010000163 DAOSCH010000000
VERSION     DAOSCH010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20979_length_3015_cov_5.638176"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..1197
                     /locus_tag="PLZ54_08745"
     CDS             325..1197
                     /locus_tag="PLZ54_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOJ67046.1"
                     /translation="MMKYFAPSELLINDDGSIFHLHLKPEHLADKIILVGDPGRVEKV
                     AAHFDSKECSVSNREFNCITGTYQNKRITVVSTGIGTDNIDIVMNELDALANIDLKNR
                     TEKADFRQLTIVRIGTSGGMQPEIPLGSFLISEKSIGIDGLLSFYARRDEVCDLDFEK
                     AFKKHTKWNPQLASPYVVDNDMELIERIGKDDMLRGVTISANGFYGPQGRVLRLDLHD
                     SHLNDKIESFRYKDYKITNFEMESSAIAGLSKLMGHKAMTVCCIIANRRVESANTNYQ
                     PHIEKLIQTVLDRI"
     gene            1216..2613
                     /gene="mnmE"
                     /locus_tag="PLZ54_08750"
     CDS             1216..2613
                     /gene="mnmE"
                     /locus_tag="PLZ54_08750"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOJ67047.1"
                     /translation="MNTASTICAISTPPGTGGIAVIRVSGQNAFSCVNKFFVPRKKDT
                     DIFQQSPYTLNLGSFMNARKETIDDVLIGIFRAPHSYTGEDVVEISCHGSVYIQQQIL
                     QTLIDAGCSLAKPGEFTQRAFLNKKMDLSQAEAVADLIAADSAASHRMALNQMRGGIS
                     EELKNLRTRLLDFVSLTELELDFSEEDVEFADRSQLMALANEIEELILKLSNSFKVGN
                     ALKNGIPVAIVGETNVGKSTLLNVLLNEDKAIVSDIHGTTRDAIEDSVNIDGITFRFI
                     DTAGIRETKDEIETLGIERTYQKIEQASIILWLIDCTQLSERIETLTEKIAKKIQDKK
                     FILIFNKIDKITDEEREVLNQFFEHYSGERIYLSAKKRINIHQLQKLLVKAAQLPEVQ
                     PGDVVVSNVRHYEALQNALKAIQRVKEGIQNGISGDFLSQDLRECMFFLGEITGEISN
                     DEILGNIFSKFCIGK"
BASE COUNT         1006 a          504 c          548 g          957 t
ORIGIN      
        1 attcataaat aaataaatag aaattaatct gaatatattc attcgtgttt gtgtatgcaa
       61 tatatgtgag tttcataatt aattgatttt caatagctca ataaacgatt tttaaaaaga
      121 aattatttca cttctcctct cctatttttg acattctatt taatatattt atattcagtt
      181 atttatagta aaattttaat tgaattttat tcaaattcgc ctttttaata aaaaaaaaat
      241 gagttttgaa ttaaaaataa gttatatttg cctttggaat tgcttctgca acaataaaac
      301 gaattaattt tttaaaacag aaatattatg aaatactttg ctccttctga acttcttatc
      361 aatgatgatg gaagtatttt tcatctgcat ctgaaacccg aacatttggc cgataaaatt
      421 attttggttg gagacccggg acgtgtagaa aaagttgctg cacattttga ttcgaaggaa
      481 tgttcagttt caaacagaga atttaactgt atcacaggta cttatcaaaa taagcgtatt
      541 acggttgttt ctaccggcat tggaacggat aacattgaca ttgtcatgaa tgagctggat
      601 gctttggcca acattgattt gaaaaacaga accgaaaaag cagatttcag acaactcacc
      661 attgttcgga ttggaacttc cggcggaatg caacccgaaa ttcctttggg aagttttctt
      721 atttctgaaa aatccatcgg aatagatggt ttattgagtt tttatgcccg cagagacgaa
      781 gtttgtgatt tggattttga aaaggctttt aaaaaacata cgaaatggaa tccgcaattg
      841 gcttctccct acgttgtaga taatgatatg gaattaatcg aacgaatagg gaaagatgac
      901 atgttaagag gtgtaaccat ttctgccaat ggattttatg ggccacaggg aagagtactc
      961 cgtctcgatt tgcacgacag tcatctgaat gataaaattg aaagtttccg atacaaagat
     1021 tataaaatta ccaatttcga aatggaaagt tctgcaattg ccggtctctc caaactcatg
     1081 ggacataagg ccatgacggt gtgctgcatt attgccaatc gcagggtaga atcagccaat
     1141 acaaattatc agccacatat tgaaaaactc attcagactg ttttagacag aatttaacga
     1201 aatttttatt tcttaatgaa cacagcatct acaatttgtg caatttctac tccacccggc
     1261 acaggaggca tcgctgtcat ccgtgtgtcg ggtcaaaatg ctttttcttg tgtcaataaa
     1321 tttttcgttc caagaaaaaa agatactgat atttttcaac aatcgcccta tacgctcaat
     1381 ctgggcagtt ttatgaatgc tcgaaaagag acaattgacg atgtgttgat tggaattttt
     1441 cgagcacctc actcctacac cggtgaagat gttgtggaaa tatcgtgtca tggttctgtg
     1501 tacatacaac agcaaatttt acagacatta atcgacgcag gctgttcatt agccaagccg
     1561 ggagaattta cgcaaagagc ttttttgaat aaaaaaatgg atttgtcgca agctgaagcg
     1621 gttgccgatt tgattgctgc cgattcggcg gcctcgcacc gaatggcatt gaaccaaatg
     1681 agaggtggta tttcggagga attgaaaaat ttacgtacac ggttgctcga ttttgtttca
     1741 ttgactgagc ttgagctcga tttttcggaa gaagatgtag aatttgccga tcgttcgcaa
     1801 ttaatggcac ttgccaatga aatagaagaa ttaattctaa aactttctaa ttcgttcaaa
     1861 gttggaaatg ctctaaaaaa cggcattccg gtagccattg ttggcgaaac aaatgtaggc
     1921 aaatctacgc tgctaaatgt tttgctcaat gaagacaagg ctattgtttc cgatattcac
     1981 ggaacaacac gagatgccat cgaagattcg gtgaatatag acggaataac ttttcgcttt
     2041 attgatacag ccgggatacg tgaaaccaaa gatgaaattg aaacactcgg cattgaacga
     2101 acgtatcaaa aaattgaaca agcttcgatt attctatggt taattgattg cactcaactt
     2161 agtgaacgca tcgaaacgct tacagaaaaa attgcaaaaa aaattcaaga taaaaaattt
     2221 atactgattt tcaataaaat agacaaaatt acagacgaag aacgtgaagt tttaaatcaa
     2281 ttttttgaac attattcggg cgaacgcatt tatctttccg caaaaaaaag aatcaacatt
     2341 catcaattac aaaaattact ggtaaaagct gcccagttgc ccgaggtaca acccggcgat
     2401 gtggtggtca gtaacgtcag acattacgaa gcactgcaaa atgctttaaa agccattcag
     2461 cgagtgaaag aaggcattca aaacggaatt tccggcgatt ttctttcaca agatttacgc
     2521 gaatgtatgt tttttcttgg agaaattacc ggcgaaatct ccaatgacga aattttgggc
     2581 aatattttca gtaagttttg tatcggaaag taacccccct aaacaaacaa ataacaaaac
     2641 aaacaaaaaa catattaaaa tgcatataat tagcatttta gtaaaagtaa atttattttt
     2701 gttgattgtt ttttatttga atttttatca tatacttgta ccttatttgt acctcgaact
     2761 ataaaaggta ttttttcact gattttgtac ctcaaatggt ttacaaagtt tacactttgg
     2821 tacaattttc atcagaatat aatttatttt ttaaaggtct gatggaaccc acatgtctga
     2881 atattatatt tattcgtgtt tgtgtatgta atacatgtgg gtttcatatt aaataacctc
     2941 tctcctactt ttcaagccga aaaaatttac gatacgagca tttgataacg gctgtttcca
     3001 gagacgtagc atgct
//
LOCUS       DAOSCH010000164         2944 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_21702_length_2944_cov_5.389408, whole genome shotgun sequence.
ACCESSION   DAOSCH010000164 DAOSCH010000000
VERSION     DAOSCH010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21702_length_2944_cov_5.389408"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1970)
                     /locus_tag="PLZ54_08755"
     CDS             complement(<1..1970)
                     /locus_tag="PLZ54_08755"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ67048.1"
                     /translation="MKKIISFFIVLMLIFSAKAVDFMGIKIYINPGHGGYNGANDRNV
                     QTIPFALGDTLGFWESASNLTKGLALRDLLQAANATVYMSRTQNREEDDRALSEIAEE
                     ANANNVDAFLSIHSNALGTNTGTNYLLLLYHGYDDQPTVAASLPMAQTAWPRLISNQL
                     TVWTHYTTSTNIRGDFSFYGNTSGLGVLRPLTVPGFLSEGSFHDYLPETHRLLNKDYR
                     KLESNNFYRYFCDYFQTNLPSTGIIAGFAKSSNEKMNNPLYIYKAFTHDEKVPINGAK
                     VKLMNQNGDSLNVYYVDTLYNGIFAFYNLAPGTYKLHLEAKDYQPKDTTVTVTAASTT
                     YAKMFLPNLNVVIPKDTTPDYPDPIQEAGVLPMKHYNFGDQEVVVPEWLNQQPIKKVL
                     YRDEKLYVLTTEPKITIYNAQSLAKIREMDLSGIEGGVNLISDIAFTSDGYLLACNKD
                     TISLPENKGRYFKVYTWDNDSVAPSLLFKTQQQANWSNGIMGETFAVSGPRWKCTIYV
                     PSVTTGSSKAIRIVGYLYEEGNALGYKYMMDAANYTEALWGENMKFTISPSGKDHFIL
                     DSEKVLPVEYQFDWNAADRSPLIKKGEFSEQNGFNLAVCASGIHFFRHAQHTFMVSPT
                     CDADTTKVGAVLFDVTNGIQQSLKVSEKLPANG"
     gene            complement(1986..2885)
                     /locus_tag="PLZ54_08760"
     CDS             complement(1986..2885)
                     /locus_tag="PLZ54_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007483621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67049.1"
                     /translation="MRKLLLLLVLGAQTIFAQNIQVTAVSPINIGESGGMYHPVFSPT
                     GEYLLFTSENYEGLKMLSFTDGQVKTLTTEKGAGYGVKISKDGKTIAYRKNELINQRL
                     FSSVKLYSLSEGKQQQLVAPTREPLAVGFSGNKPLLVKNRQLVKSQLSAVEIVPIVTI
                     EDRKMVLYTGNSRKILTPNGENASYIWPSISPDGKRIVYVAAGKGTFVCDLNGNNVAA
                     LGKLNAPQWINNQWLVGMNDIDNGEILLSSSLVAASIDGKVYQTLTTPDLIAMYPAVS
                     PDGKQIVFNTERGDLYLLNVVLK"
BASE COUNT          874 a          601 c          564 g          905 t
ORIGIN      
        1 ccattagcag gcaatttttc ggaaactttc aacgattgct gaatgccatt ggttacatca
       61 aagagcacag cacccacttt cgttgtatcg gcatcgcaag taggcgaaac cataaaggta
      121 tgttgtgcat ggcggaagaa atgaatgccg gaagcacaga cagcaaggtt gaatccattc
      181 tgttctgaaa attcaccttt tttaattaaa ggagaacgat cggctgcatt ccaatcaaac
      241 tgatattcga ctggtaaaac tttttcgcta tccagaataa aatgatcttt cccacttgga
      301 gaaatagtaa atttcatatt ttctccccac aatgcttcgg tgtaattggc agcatccatc
      361 atatatttgt agcccaaggc atttccttct tcataaaggt aacctacaat gcgaatggct
      421 ttggatgatc cggtggtaac tgatggtacg taaatagtac atttccagcg gggaccgcta
      481 acagcaaatg tttcgcccat aatgccattt gaccagtttg cctgttgttg cgtcttgaaa
      541 agcaatgaag gagctaccga atcgttatcc cacgtataaa ctttaaaata acgacctttg
      601 ttttcgggta aagaaatcgt atccttgttg caggccaaca aataaccatc ggaagtaaaa
      661 gcaatatcgc taatcaggtt tacgccacct tcaatgccgg ataaatccat ttcgcgaatt
      721 ttagcaagtg attgcgcatt gtaaatagtg atttttggtt ctgtggttag cacatataat
      781 ttttcatcac gatacaaaac ttttttaata ggttgctgat tcaaccattc tggaacaact
      841 acttcttgat ctccaaaatt ataatgcttc attggcagta caccggcttc ctgtatcgga
      901 tcaggataat caggcgtggt atcttttgga attaccacat ttaagttagg taaaaacatt
      961 ttggcataag tagttgatgc tgccgttacc gtaacagttg tatcctttgg ctgataatcc
     1021 tttgcttcca aatgcagttt gtaagttccg ggagctaaat tgtaaaaagc aaaaattccg
     1081 ttgtacagcg tatctacata atatacattc agtgaatctc cattctgatt catcaatttc
     1141 accttagctc cattaatagg tactttttca tcatgggtaa aagccttgta gatgtacaaa
     1201 ggattgttca ttttttcatt ggaacttttg gcaaaccctg caataatacc tgtagaaggt
     1261 aaattggttt gaaaataatc gcagaaatac ctgtaaaaat tattagattc caattttctg
     1321 taatctttat tgagcagccg gtgggtttcc ggtaaataat cgtggaatga accttcggac
     1381 aaaaatccgg gaacagtcaa agggcgaagg acgcccagtc cactggtatt tccataaaat
     1441 gagaaatcgc cccgaatatt agtagaggtg gtgtaatgtg tccaaactgt aagttgattt
     1501 gaaatcaaac gaggccaggc ggtttgagcc attggtaaac tcgctgccac agtgggttga
     1561 tcatcataac cgtgatacaa aagtaataaa taattggtgc ctgtattggt tcccaatgca
     1621 ttgctgtgta tggaaagaaa ggcatccacg ttgttggcat tggcttcttc ggcaatttcc
     1681 gacaaagccc tatcgtcttc ttccctgttt tgagtacgcg acatgtaaac ggtagcattg
     1741 gctgcctgca acaaatcgcg tagtgccaat cctttggtca aattacttgc cgattcccaa
     1801 aaaccaagtg tgtcgcccaa tgcaaagggt atggtctgaa catttctgtc gtttgcccca
     1861 ttgtatcctc catgacccgg gttgatataa attttaattc ccataaaatc aacagctttt
     1921 gcagaaaaaa tcagcatcaa aacgatgaaa aatgaaatta tttttttcat ggtgtttcct
     1981 cctccttatt ttaaaacaac attaagtaaa tataaatcac ctctctcagt attaaataca
     2041 atctgctttc catcaggaga aacagcagga tacatagcta tcaaatcagg tgtagtgagt
     2101 gtttgataca cttttccatc tatcgatgct gctaccagag aagaagaaag gagtatttcc
     2161 ccgttgtcaa tgtcattcat tcccaccagc cattgattgt tgatccattg tggcgcattc
     2221 agtttaccca gcgccgcaac attgttcccg ttcaagtcgc aaacaaacgt tccttttcct
     2281 gcagcaacat agacaattcg tttcccatcg ggcgaaatgg aaggccaaat atagctcgca
     2341 ttttcaccat tcggagtaag aattttacgc gaatttccag tataaagaac cattttacgg
     2401 tcttcgatgg taacaatagg tacaatttcg acggcagaaa gctgactttt taccagttga
     2461 cgatttttta ccaataaagg tttgtttcct gaaaaaccta ccgcaagagg ttctcgcgtg
     2521 ggagcaacaa gttgttgttg ttttccttca gaaagcgaat acaatttaac ggaagaaaat
     2581 agccgctggt taatcaattc gtttttcctg taggcaatgg tttttccatc tttgcttatc
     2641 ttaactccat agcccgcacc tttttcggtt gtcaaagttt ttacctgccc atcagtgaac
     2701 gaaagcattt tcagtccttc ataattttca gaagtgaaaa gcaaatattc tcccgtcgga
     2761 ctaaataccg gatgatacat gccgccactt tctccgatgt ttatcggaga aacagctgta
     2821 acctgaatgt tttgagcgaa aattgtctga gcacccaaaa ccaataacag tagtagtttt
     2881 ctcatgatat taacatttta aaaaattaat tttggttttt tcggtggtaa atgtagaata
     2941 tttt
//
LOCUS       DAOSCH010000165         2941 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_21733_length_2941_cov_3.756757, whole genome shotgun sequence.
ACCESSION   DAOSCH010000165 DAOSCH010000000
VERSION     DAOSCH010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21733_length_2941_cov_3.756757"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="PLZ54_08765"
     CDS             complement(<1..555)
                     /locus_tag="PLZ54_08765"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOJ67050.1"
                     /translation="MTSNIESERVLEKQRWKFAIILPSIVCVLWILCFVLERGMGWDF
                     HKAGIYPRKLENIWSIFTYSFIHADWKHLMNNVLSFFILGICLYYFYSEVANRVLIIS
                     AVLSGLILWCIGRENFHIGASGIIYSLAFFLFFSGIIRKHIPLIAISLIVVFIYGSMV
                     WHLFPWLPDDPVSWEGHLSGAATGI"
     gene            945..2885
                     /locus_tag="PLZ54_08770"
     CDS             945..2885
                     /locus_tag="PLZ54_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOJ67051.1"
                     /translation="MSYLSFDKSLLINLERSLSKEILRTNRAGTYNSSTLIDCNTRKY
                     HGQLVVPVPELGEANHVLLSSLDETIVQRGIEFNLGVHQYEGNNFYPNGHKYIRQFDC
                     EIIPRTIYRVGGVILSKERMLVSFEPRVLFKYTLLEAHSPTLLRFKPFLAFRNVHELT
                     HQNPEANCDFQLVSNGIAMKLYENYPTLFMQLSKKNVYQHFPDWYKNIEYYKERERGF
                     EYREDLLVPGSFEIPIKKGESVIFSAGISEVSTSSLKKLWDKELEKRNPRCDMFSALK
                     NSAQQFYKREGDKTYLLAGYPWFTARARDEFVALPGCTLSIGRMEYFDAIIETAIPEI
                     ESFINENKVSSKLKEIDAPDVLLWFVWTIQQYARHTSLSETAQRFGKLVASIISFVRK
                     QRHPNLFLHNNGLLYVNGLEKPATWMNAVENGRPITPRTGYVVEINALWYNALKFSSQ
                     LLGEMGEEMEANVIDYQSGILRESFIKTFWNGNYLYDFVNGNYNDKEVRPNMIYAVGL
                     PFSPLEKSQQKLILDIVTKELLTPKGLRTISPKSGMYRPVYIGGQLERDRNYHNGPVW
                     PFTFGAFVEAYLKIYKMSGKSFIERMMIGFEAEMKELCVGTLSELYDGNPPFKGHGGM
                     SFAMNVGEVLRVMEILKEYKED"
BASE COUNT          967 a          543 c          553 g          878 t
ORIGIN      
        1 gataccggtt gcggctcccg ataaatgtcc ttcccatgaa accggatcgt ccggcaacca
       61 ggggaaaaga tgccaaacca tactaccata aataaacact acaatcaacg aaatagctat
      121 caaaggaata tgttttctga ttattccgct gaaaaacaga aaaaatgcca gcgagtaaat
      181 aatgccactg gcgccaatgt gaaaattttc gcgtccgata caccacaaaa tcaatccact
      241 gagcacagcc gatatgatta gcactcgatt ggcaacctca ctgtaaaaat aatacaggca
      301 tattcccaaa ataaaaaaag aaagaacgtt attcattaaa tgcttccaat cggcatgaat
      361 gaaactgtaa gtaaatatac tccaaatgtt ttcgagtttt cgcggataaa tgccggcttt
      421 atgaaaatcc caacccatgc cgcgctccaa tacaaaacat aatatccaca aaacacatac
      481 gattgaaggc aaaataatgg cgaatttcca ccgttgcttc tccagaactc tttcactctc
      541 gatgttgctc gtcatttcgc aactctgttt taaataacgt cttccaaaca ctaaaaatta
      601 catcaaaggt aaaaattttt ttgtttgaga agataatttt taactgtcaa atgaactttc
      661 tctacgattc agccatcatt ttttttgatg aattttataa aaatttgaaa aacaacaaat
      721 tgcatgtatc aatgaaattt ggatttgttg ggaaccaaga aatttttttc gtcaccttga
      781 aaaaattatc aacttccttt tttttaattg gaaattttcg ataaattgca agcaatatta
      841 aaatctatct aaggattgat ttttgtaatg tttctaattt acattccttt ttaaaagaaa
      901 attgtattta attgataatc agatagtcta agaataacaa tcagatgagt tatctaagtt
      961 tcgacaaatc cttgttaatt aaccttgaac ggtctctttc gaaggaaatt ctccgtacca
     1021 atcgtgcagg cacttacaac agttcaacct tgatcgattg caacaccaga aaatatcatg
     1081 ggcaattggt agtgcccgta cccgagttgg gcgaagccaa tcatgtattg ctctcttcgt
     1141 tggacgaaac cattgttcaa cgtggaattg aatttaattt gggggtgcat cagtacgaag
     1201 gcaacaattt ttatcccaac ggccacaaat acattcgtca attcgattgc gaaattattc
     1261 caagaacaat ttatcgtgtg gggggagtaa ttctttcaaa agaacgcatg ctggtttcat
     1321 ttgaaccacg tgtccttttt aaatatactt tgttggaagc tcattcgcca actttgttac
     1381 gctttaagcc ttttctggct tttcgaaatg ttcatgaact tacccatcag aatccagaag
     1441 ccaattgcga ttttcagctt gtttccaacg gtatagccat gaagctttat gaaaattacc
     1501 ccacgctttt catgcaactt tccaaaaaga atgtttatca gcatttcccc gactggtata
     1561 aaaatataga atattacaaa gaaagggaaa gaggttttga atatcgagaa gacttgttgg
     1621 tgccgggttc ttttgaaatt cccataaaaa aaggagaatc cgttattttt tcagcaggaa
     1681 tatcggaagt ctcaacttca tcactgaaaa agctttggga taaggaattg gaaaaaagaa
     1741 atccacgctg tgatatgttc agtgcactga aaaattctgc ccaacaattt tacaaacgcg
     1801 aaggcgacaa aacttattta ttggcaggtt atccctggtt tacggcacgg gcaagagatg
     1861 aatttgttgc tttacccggt tgtactttgt cgattggcag aatggaatat ttcgatgcca
     1921 taattgaaac ggccattcct gaaattgaaa gttttattaa cgaaaataaa gtcagctcca
     1981 aactgaaaga aatcgatgca cccgacgttt tgctgtggtt tgtatggacc attcagcaat
     2041 atgctcgtca cacttcactt tcagaaacag cgcagcgttt cggaaaattg gttgcttcaa
     2101 ttatttcttt tgttcgcaaa caacggcatc ccaatctttt tctccacaac aacggcttgc
     2161 tttatgtcaa tgggttggaa aaacctgcta catggatgaa tgcggtagaa aatggcagac
     2221 ccattactcc tcgaacaggt tacgtagttg aaattaatgc actgtggtat aatgctttga
     2281 aattttcttc tcaacttctt ggtgaaatgg gagaggagat ggaagccaat gtaatagact
     2341 atcagtcagg catattaaga gaatcgttca taaaaacttt ttggaatgga aactatcttt
     2401 acgactttgt taacggcaat tacaatgaca aggaagttag accaaacatg atttatgcag
     2461 tgggcttacc attctctcct cttgaaaaat cacagcagaa gttgattctc gatattgtaa
     2521 ccaaagaatt gttgacaccg aaaggattaa gaaccattag ccctaaaagt ggtatgtatc
     2581 gtccggtgta cataggtgga cagttggaaa gagaccgaaa ttaccacaac gggcctgttt
     2641 ggccttttac ttttggtgct tttgttgaag cttatttaaa aatttacaaa atgagtggaa
     2701 aatcttttat cgaaagaatg atgattggct tcgaggcaga aatgaaagaa ttgtgtgtcg
     2761 gtacactttc cgaattatat gacgggaatc caccttttaa gggacacggc ggaatgtctt
     2821 ttgccatgaa tgtgggagaa gtattgcgtg taatggaaat tttgaaagaa tataaagaag
     2881 actaatacca ctgaatttgg tgtaaacgga aagtttagag gtgagtatat acgatgaaaa
     2941 a
//
LOCUS       DAOSCH010000166         2852 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_22688_length_2852_cov_5.670361, whole genome shotgun sequence.
ACCESSION   DAOSCH010000166 DAOSCH010000000
VERSION     DAOSCH010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22688_length_2852_cov_5.670361"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..624)
                     /locus_tag="PLZ54_08775"
     CDS             complement(178..624)
                     /locus_tag="PLZ54_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HOJ67052.1"
                     /translation="MEKSKLMNEKQKLLDMRYMRMARIWAENSYCRRRQVGALLVKNK
                     MIISDGYNGTPAGFENNCEDEDDNSKPYVLHAEANAITKVARSHNSSDGATLYVTASP
                     CMECAKLIIQAGIKRVVYGEHYRIMDGIELLQRAGIEVVYLGDDNI"
     gene            complement(630..1997)
                     /locus_tag="PLZ54_08780"
     CDS             complement(630..1997)
                     /locus_tag="PLZ54_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOJ67053.1"
                     /translation="MQRVHFIAIGGAAMHNLAIALSKKNNYKITGSDDEIFEPSYSRL
                     KEHGLLPEKTGWFPERIHKGLNAVILGMHAREDNPELLKAKELKLKIYSFPEFLYQQT
                     RNKTRIVVGGSHGKTTTTAMILFVLNKLKIEADYMVGAQIEGFDNMVRLSYDSRIAVF
                     EGDEYLTSPLDPRPKFHLYHPHIAVLTGIAWDHMNVFPTFENYLEQFKKFTDLIELQG
                     RLIYFDGDENLNKIVQQLRRDIVPFPYHTPPYEVRNEITWLKIKNKEIPLKIFGEHNL
                     QNLTAAHIACHQIGISDDDFFTAISEFSGASNRLEKLTENNDTVVFKDFAHSPSKVKA
                     TIKAVRQQYPHRRLIACLELHTFSSLMEDFLPQYARCMDEADVAMVYYDPEIIEQKRL
                     KQFEPEQVKTSFGKADLQVFTNKNDLEKALKSLDYKNSALLLMSSGNFSGLNVNDIVN
                     EKISL"
     gene            2098..>2852
                     /locus_tag="PLZ54_08785"
     CDS             2098..>2852
                     /locus_tag="PLZ54_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOJ67054.1"
                     /translation="MKIHKIEAGFFDCDGGAIFGVVPKKVWQKRYPCNEENFCKLAMR
                     CLLIDTGEKLVLIDSGTGDKQLDYLKYYNFKGVVNFELALQQLGYSCGDVTDVILTHL
                     HFDHCGGCTYYDSDRKNVLLTFPNAVHWVGERQWINFLEPNVREGDSYFPENMLPVQQ
                     AGKLKLVKENSFLCPEIELRIFNGHTVGQLVSYIQYLGKTWIYVGDVIPVAPSIPLAW
                     ISAFDAYPVTSMEEKKILLQEAEEKHQILLFEH"
BASE COUNT          811 a          523 c          603 g          915 t
ORIGIN      
        1 cttatttcgt tgcaaatcaa taacctgatg agagcccgtt ttagattgtt ttctttaagt
       61 gataaaaatt aaagcagtat tttaatttgt cagtccgtcg tgccacagat aggcttccgt
      121 ttatcgtaaa ggtttaaaat aatttttgta atccataata caaaaaagag gtgaatttta
      181 tatgttatca tcgcccagat aaaccacctc aatgccggca cgttgtaaaa gttcgatgcc
      241 atccatgatt cggtaatgtt cgccatacac aactcttttg atgcctgcct gaattattaa
      301 tttggcgcat tccatgcagg gagaggcggt aacgtaaagc gttgcaccgt cgctgctatt
      361 gtgcgaacgt gctacttttg ttatggcatt tgcttcagca tgaagcacgt aaggttttga
      421 attgtcgtcc tcatcttcac agttattttc aaatcctgca ggtgtgccat tgtagccatc
      481 cgaaataatc attttatttt tcaccagtaa agcacccacc tgcctgcgac gacaatagga
      541 gttttctgcc catattctgg ccatgcgcat ataccgcata tccagcaatt tttgtttttc
      601 attcataagt tttgattttt ccatctgttt cacaatgaaa ttttttcatt gacaatgtca
      661 ttcacattca aacccgaaaa gtttcccgac gacatgagta aaagggccga atttttataa
      721 tccaaagatt ttaaagcttt ttccaaatca ttcttattcg taaaaacctg taagtctgct
      781 tttccgaaac ttgtttttac ctgttctggc tcaaattgtt tcagtcgttt ttgttcaata
      841 atttcagggt cgtaataaac catagccaca tctgcttcgt ccatgcatct tgcatattga
      901 ggaagaaaat cttccatcag actgctgaag gtgtgtagtt cgaggcaggc tattaatctg
      961 cgatgaggat attgttgccg aacggctttg atggtagctt taactttgga aggagagtgg
     1021 gcaaaatcct tgaatacgac ggtatcattg ttttcggtca atttttccaa tcgattagaa
     1081 gcgccggaaa attcggaaat agccgtaaaa aaatcgtcgt cggaaatgcc aatctgatga
     1141 caggcaatgt gtgcggcagt caagttttgc aaattgtgtt ctccgaaaat ttttaaagga
     1201 atctccttgt ttttaatttt aagccaggta atttcgtttc ttacttcata aggtggtgtg
     1261 tgataaggaa aggggacgat gtcgcggcga agttgctgaa caattttatt caggttttcg
     1321 tctccatcaa aataaattag tcttccttgc aattcaatta aatcggtgaa ttttttaaat
     1381 tgttctaaat agttttcaaa agttggaaaa acattcatat gatcccaggc aatgccggta
     1441 agtactgcaa tatgtggatg atataaatga aatttagggc gtggatcgag cggggaggta
     1501 aggtactcgt ctccttcaaa aactgctatg cgcgaatcgt aagaaagcct gaccatgtta
     1561 tcgaaacctt caatttgagc gccaaccata taatcggctt caattttcag tttgttcagc
     1621 acgaataaaa tcatggctgt tgtggtggtt tttccgtgac ttccgcccac cacaatacgc
     1681 gttttgttgc gtgtctgctg ataaaggaat tcagggaaag aataaatttt caattttaat
     1741 tcttttgctt tcagcagttc aggattatct tcgcgggcgt gcatgccgag aattacagca
     1801 ttcaaaccct tgtggatgcg ctcgggaaac cagcccgttt tttccggcaa cagtccatgt
     1861 tctttcaatc gggaataaga aggttcaaaa atttcatcat ccgaacctgt aattttatag
     1921 ttgttttttt tactgagtgc gatggctaaa ttatgcatgg ctgctccgcc aatggcaata
     1981 aaatgaactc gctgcataag gttggaaggt tttttaaatt ttattgctac ttttgtggaa
     2041 agtatttttt ataatgcaaa aatacgaaga aaatacgaat cgaaagctac tttgaatatg
     2101 aaaatacata aaatagaagc cggttttttc gactgcgatg gtggagccat ctttggtgta
     2161 gttcccaaaa aggtttggca aaagcgttat ccgtgcaatg aggaaaattt ttgtaagctg
     2221 gccatgcgtt gcctgttgat cgatacaggc gaaaaacttg ttttgattga cagtggtacg
     2281 ggcgacaaac agctggatta tttgaaatat tacaacttca agggagttgt gaattttgaa
     2341 ttggctctac agcaattggg atacagttgc ggagatgtaa ccgatgtaat cttgactcat
     2401 ttgcatttcg accattgcgg aggatgtact tattatgatt cagatcggaa aaatgtattg
     2461 ctcaccttcc caaatgccgt tcactgggtg ggagaaaggc aatggataaa ttttctggag
     2521 cccaatgtga gggaaggaga ctcttatttt cctgaaaata tgctgcctgt gcagcaagca
     2581 gggaaattga aactggtcaa ggaaaacagt tttctctgtc ccgaaattga acttcgtatt
     2641 ttcaacggac atacagtagg gcaattggtc agttatattc aatatttggg gaaaacatgg
     2701 atttatgtgg gtgatgtaat tcctgtagca ccatccattc cattggcttg gatatcggct
     2761 ttcgatgctt atccggtaac ttcgatggag gagaagaaaa ttcttttgca ggaagctgaa
     2821 gaaaaacatc aaatcctcct attcgagcat ga
//
LOCUS       DAOSCH010000167         2827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_22970_length_2827_cov_6.834416, whole genome shotgun sequence.
ACCESSION   DAOSCH010000167 DAOSCH010000000
VERSION     DAOSCH010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22970_length_2827_cov_6.834416"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1832
                     /locus_tag="PLZ54_08790"
     CDS             <1..1832
                     /locus_tag="PLZ54_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67055.1"
                     /translation="IFKYDLAEFRKDKYSKEINLIRSLYDEQDRIIQALILNLYRQEL
                     ATSNNINLKPVILFKAKKTIKESEQNKENFHKLIDDFSVAMVEKIQKTSTVPIVQKAF
                     RFFEDKGISANEIVKRIQANFKPENCLSANNDAEAERNQILLNTLEDENNSIRAVFAV
                     QKLNEGWDVLNLFDIVRLYEDRDGKDGKPGKTTLSEAQLIGRGARYFPFALEEGQDKF
                     TRKFDDDISNDLKILEELYYHTKEDSRYISELKKALVDSGIYEDDENLVTKQLTLKLD
                     FKGTDFYRDGHVFFNKKIPKSFDNIKSFADLGVKKTNYRHTLSSGVGRMSGAFTESET
                     SETEAIKTKDVKLTDIPKNTIRFALSQNPFFYFDNLSHYFPSIGSLSNFIDSTDFLAG
                     LEITFSGTANRLKEISHFDYLQALNGLLQSIEADIKSNSTEYEGSDYIKEPIHKVFKD
                     KEIKVYKDSERADGQETLVANEPWYVYNANYGTSEEKKFVELFSRRFEGLNQKFENIY
                     LIRNEREIKIFDKLGRAFEPDFLLFCKQRDGEQMTFQVFIEPKGEHLKGHDKWKEDFL
                     KEIRTEQKTIKIHTDTYLITAVPFYNYNNENEFKTTLENTLNE"
     gene            2063..2554
                     /locus_tag="PLZ54_08795"
     CDS             2063..2554
                     /locus_tag="PLZ54_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67056.1"
                     /translation="MDRILLRNPLRHIHSTDDLKKIKNWDDFVDLIHNTWRKKNNSNN
                     EIVFRSGGYADVMYFSNNIYVASVYGGVLNAYILNFKSPYILDCKNSSWHNINKPEIM
                     KEIPSDIVDTDSIVDFILKEKLNYDGIIFLNLYEGSGANVFGPSNVYVSLDKKTVVNL
                     TNN"
     gene            2587..2673
                     /locus_tag="PLZ54_08800"
     tRNA            2587..2673
                     /locus_tag="PLZ54_08800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2621..2623,aa:Ser,seq:gct)
BASE COUNT         1053 a          465 c          518 g          791 t
ORIGIN      
        1 taattttcaa atacgacctt gccgagtttc gtaaagacaa gtattccaaa gaaattaacc
       61 ttatccgttc tttgtatgac gagcaagaca gaatcattca ggccttgatt ttgaatttgt
      121 atcgtcagga attggcaact tcaaacaata ttaatttgaa gcctgtaatt ctgtttaaag
      181 ccaaaaagac aataaaagaa tcggagcaga acaaagaaaa ctttcacaaa ctgattgatg
      241 atttttctgt ggcaatggtg gagaaaatac aaaagacttc aaccgttcca atcgttcaaa
      301 aggctttccg attttttgaa gacaaaggca tttcagccaa cgaaatagtt aaacgcattc
      361 aagccaattt caaacccgaa aattgcctaa gtgccaacaa tgacgcagaa gccgaacgaa
      421 accaaatatt gctcaatacc ttagaagatg aaaacaattc aattcgtgcc gtttttgccg
      481 ttcagaaact aaacgaaggt tgggacgttt tgaatttgtt tgacattgtt cgcctttacg
      541 aagaccgaga cggaaaagac ggtaaaccag gcaaaactac actttcggaa gcccagttaa
      601 ttggtcgtgg tgcacggtat tttccgtttg ccttagaaga agggcaggac aagtttaccc
      661 gaaaatttga tgatgatatt tcgaacgact taaaaatatt ggaagagctg tattaccata
      721 ccaaggaaga tagccgatac atttcagaat tgaagaaagc ccttgtagat tcgggtattt
      781 atgaagatga tgaaaacctt gttaccaagc aactgacatt aaaattagac tttaagggca
      841 cggattttta cagagatgga cacgtttttt tcaataagaa aattccaaag agttttgaca
      901 acataaaatc gtttgccgat ttaggagtta agaaaaccaa ctatcgacac actttatctt
      961 caggtgttgg cagaatgtcg ggagctttta ccgaatcgga aacttcagaa acagaagcta
     1021 ttaaaaccaa agatgttaag ctgactgata ttccgaaaaa tacgattcgt tttgcattaa
     1081 gccaaaaccc atttttctat ttcgacaatt tatcacatta ctttccaagt attggttcac
     1141 tttcaaactt cattgacagc acagactttt tagcaggttt agaaatcact tttagcggaa
     1201 cagcaaaccg attgaaagaa attagccatt ttgactattt gcaagcctta aacggacttt
     1261 tgcaaagcat tgaagcagat ataaagagca attcaacaga atacgaaggt tcagactata
     1321 ttaaagaacc gattcacaaa gtatttaagg acaaggaaat caaagtttac aaagacagcg
     1381 aacgtgcaga cggacaagaa actttggtag ccaacgagcc ttggtatgtc tacaatgcca
     1441 actacggaac aagtgaagaa aagaaatttg tagaattgtt ttccagacga tttgaagggc
     1501 tgaatcagaa gtttgaaaac atttacctaa tccgaaacga aagagaaatt aaaatcttcg
     1561 acaaactcgg acgagctttt gaacctgact ttttgttgtt ctgcaaacaa cgtgacggag
     1621 aacaaatgac ctttcaggtt ttcatagaac ctaaaggaga gcatttaaaa gggcacgaca
     1681 aatggaaaga agactttttg aaagaaatca gaacagagca aaagactatc aaaatccaca
     1741 cggacactta tttgataaca gcggttccgt tctataatta caacaacgaa aacgaattta
     1801 agacaacatt agaaaacaca ttaaacgaat agaaatgcca acgcccaaca gccacacaca
     1861 ttgccaagtc gcaccagccg accctcaagc caaaacttgg caaagagtgt gtctgttcct
     1921 aacgcacgga caggaagaca atgcagcaaa aatgaaagaa aattaacgag aaaaatgaac
     1981 aacgaaacgc taacaaagtg tatatgccat aagggtttca gtgggtattc aaacgttgta
     2041 gcccgctcaa acattcgtgt cggtggacag gatactgctc cgcaatccct tacggcacat
     2101 acactcgacc gatgatttaa aaaaaataaa aaattgggac gattttgttg atttaattca
     2161 taatacatgg cgtaaaaaaa ataattcaaa caatgaaatt gtttttagaa gtggcggtta
     2221 tgcagatgtg atgtattttt caaacaatat atatgttgct tcagtatatg gtggtgtatt
     2281 gaatgcatat attttaaatt tcaaatcacc atatattttg gattgtaaaa attcaagttg
     2341 gcataatatt aataaacctg aaataatgaa agaaatacct tctgatattg ttgatactga
     2401 tagtattgtt gattttatat taaaagaaaa attaaattat gatggtatta tatttttaaa
     2461 tttatatgaa ggtagtggtg ctaatgtttt tggtccatca aacgtttatg tttctttgga
     2521 taaaaaaact gttgttaatt taacaaataa ttagtataat gagtattata gacatatatt
     2581 aacttaggag agttgggtga gtggtttaaa ccagtagtct gctaaattac tgtacgtgaa
     2641 aacgtaccga gagttcgaat ctctcactct ccgcatataa atgtttaaat taattgcaga
     2701 aaaatatgta ttttttgtaa caaaaatttt aacaattcgt aatatatata aaaaaatatt
     2761 acaaatgaca agggaagaat ttgagaaaaa atttaaaata atacaaatat ttgttgcaaa
     2821 agcagga
//
LOCUS       DAOSCH010000168         2826 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_22976_length_2826_cov_8.308914, whole genome shotgun sequence.
ACCESSION   DAOSCH010000168 DAOSCH010000000
VERSION     DAOSCH010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22976_length_2826_cov_8.308914"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(147..>2826)
                     /locus_tag="PLZ54_08805"
     CDS             complement(147..>2826)
                     /locus_tag="PLZ54_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67057.1"
                     /translation="TEEELIAEKYKNSYVVSSNNVPSKIVEDKIVTLVPPLKVRGAIK
                     WQASSDGTTWFDLSENMSQNDLKANFVSVNQQELVLSPKTPAYYRCSVQDINCEPLLS
                     DTIKVNPFGKVLYDENVNVETQSQTVKIDSIEVTIPEKIYDKDFRLTIVKLDNPPSPP
                     DSVVNIGSAYDVTVSFAETFETPLLIKLKNIDKKMINDESIEQIKAMYFDNKTRKWVY
                     YANSYISLKDSSLLFETNHLTIVIPTLIKETQQKEWEKYQRGNIIVYYRLEDIKTMNE
                     KYEQTPQPWHSGNAPLYLQDIGEFLDQVRKSFKESPNNLPVPEYFTVYVKKMEDNDGV
                     VGLLGMIKGYLTINCDIKSPTKLRSVLAHEYMHYTQDYYISANPSNIFWMEATAHLSD
                     RMVWGANTIPISESEQYLLDGRTSSNSIYDFLANSWDYWDYSILTQNLWGNIYYCYLA
                     GTFLHYMRSYREGEKLNPAALLKGTTWLGNWRNYLDSYIKKNLKSNIGDEYENFVKYL
                     LQGSNNNFTILDIPDGPNPYSYIIAQSKYKKFSDKIVYRFDKNNQTPQKDEMTFDIPY
                     LATKILMLYNNTPDRAVVVNYKRSHKPDENYKVYYGRYDDTQQQTVYVDISDSTNYNI
                     FIEAHSEKATKEKKNLCFLLFINKTNPSTFSWNTDFEASFELTATPVFDIQNLIGGWV
                     AGNDGDNLFVHTFSQNAKYGFVISGVHKAPIRSTIVSHTVNYYSSHRSEINDSSYVVN
                     VHFSDETRDEYPPDPLGTCLPTIQISEKTQRIVYNFVRGSIKIHSNEKYINQFEEKDE
                     NDKPVIYKGSEFYIDATLWVKNLYSMTILTGGDNVVFKTRNSAETQSAIEKMSYTFRD
                     VDFDLKGDPKADKTYEYQSTDYSSGDIILQLIFETK"
BASE COUNT          826 a          522 c          486 g          992 t
ORIGIN      
        1 ataaaattat ttgctacatc tacttccgcc tgtgcattgg aacgattcaa ccgcaaaaca
       61 ccaatgacat tggtcgtgcc ctgcgtagtc tggtcaaaac gtatagttcc caaattcttg
      121 gtcgattcat ctcctaaaat atataattat ttcgtttcaa atattaattg taatattata
      181 tcgccactcg aataatccgt actttgatat tcgtaggttt tatcagcctt tggatctcct
      241 ttcagatcaa aatctacatc tctaaatgta taggacattt tttctattgc cgattgtgtt
      301 tctgcactat ttcttgtttt gaaaacaacg ttatctccac ctgttagaat tgtcatcgaa
      361 tacaagtttt ttacccaaag ggttgcatca atataaaatt cacttccctt gtagataact
      421 ggtttgtcat tttcatcctt ttcttcaaac tgattaatgt atttctcatt ggaatgaatt
      481 tttatacttc cacgaacaaa attatagaca attctttgcg ttttttctga aatttggata
      541 gtcggcaaac atgtaccaag aggatctgga ggatattcgt cacgagtttc atctgaaaaa
      601 tgcacattta caacataaga ggaatcgttg atttcacttc tatggctact ataataattc
      661 acggtatgag aaactatagt tgaacggatt ggtgccttat gaactcctga aatcacaaat
      721 ccatatttgg cattctggct aaaagtatga acaaataaat tatctccatc gttacctgca
      781 acccatccac ctataagatt ttgaatatca aatacaggag tagctgtcaa ttcaaacgaa
      841 gcttcaaaat cggtattcca actaaaagtt gacggatttg ttttgttaat gaacaaaaga
      901 aaacacaaat tctttttttc ttttgtagct ttttcagaat gtgcttcgat aaaaatattg
      961 taattagttg agtccgaaat atccacataa acggtttgtt gttgggtatc atcatatctc
     1021 ccataataaa ctttgtaatt ttcatcaggt ttatgagaac gtttataatt gacgacaaca
     1081 gctctgtcag gagtattgtt gtagagcata agtatttttg ttgccagata agggatatca
     1141 aacgtcatct cgtctttttg aggagtttgg ttatttttat cgaatctgta cacaatttta
     1201 tccgaaaatt ttttatattt gctttgagca ataatatagg aatatggatt gggaccatca
     1261 gggatgtcta aaattgtgaa attattattc gatccttgca gaaggtattt tacaaaattt
     1321 tcgtattcat caccaatgtt cgatttcaaa ttctttttga tataactgtc caaataattg
     1381 cgccaatttc ccagccacgt agttccttta agcaatgcgg ccgggtttag tttttcaccc
     1441 tcgcgatagc ttcgcatgta atgcagaaaa gtgcctgcca aataacaata atagatattt
     1501 ccccacaggt tttgtgtcaa aatagagtag tcccaataat cccaggaatt agcaagaaaa
     1561 tcatatattg aattggatga tgttcgtccg tccagcaagt attgttccga ttcgctgatg
     1621 ggtatcgtat ttgcacccca aaccattctg tcagaaagat gagcagtggc ttccatccaa
     1681 aagatatttg atggattggc agaaatataa taatcttgtg tatagtgcat atattcatga
     1741 gcaagtacac ttctcagttt tgtcgggctt tttatgtcac aattgatggt caagtaacct
     1801 ttgatcattc ctaaaagacc gactacgcca tcgttatctt ccattttttt gacataaacc
     1861 gtaaaatatt ctggtaccgg caaattattg ggcgattcct tgaaagattt cctcacctgg
     1921 tctaaaaatt cgccaatatc ctgaaggtag agtggtgcat ttccgctatg ccacggttgt
     1981 ggcgtttgct catatttttc gttcatcgtt ttaatatctt cgagccggta gtaaacaata
     2041 atattaccgc gttggtattt ttcccattct ttttgttgcg tttcttttat cagagtcgga
     2101 atcacaattg ttaaatggtt agtttcgaaa agaagtgagc tatcttttaa gctgatatac
     2161 gaattggcgt aatagaccca tttacgagtt ttgttgtcga aatacatcgc cttaatttgt
     2221 tcgatacttt catcattgat cattttttta tcaatatttt tcagttttat tagaagcggg
     2281 gtttcaaaag tttcggcaaa gctgacggta acatcataag cgctgcctat attcaccaca
     2341 ctgtcgggtg gagaaggtgg attatcgagt tttacaatag tgagacggaa atccttatca
     2401 tagatttttt ccggaatggt aacttcaatg ctgtcaattt tcactgtctg tgattgagtt
     2461 tccacattca cattctcgtc atacagcact ttgccaaaag gattcacttt gatggtatcg
     2521 ctcaaaagcg gttcgcaatt aatatcctgt acactacaac gataataagc tggcgtttta
     2581 ggcgagagca ccagttcttg ttgatttaca cttacaaaat ttgccttcaa atcattttgc
     2641 gacatatttt cggaaagatc aaaccatgtg gttccgtccg atgaagcttg ccatttaatt
     2701 gctcctctaa cttttaatgg tggaacaagc gttactattt tatcttccac aatttttgaa
     2761 ggaacattat tggaagaaac cacataagag tttttgtatt tctcagcaat caattcttct
     2821 tccgta
//
LOCUS       DAOSCH010000169         2820 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_23056_length_2820_cov_5.405425, whole genome shotgun sequence.
ACCESSION   DAOSCH010000169 DAOSCH010000000
VERSION     DAOSCH010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23056_length_2820_cov_5.405425"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(377..1216)
                     /gene="prmA"
                     /locus_tag="PLZ54_08810"
     CDS             complement(377..1216)
                     /gene="prmA"
                     /locus_tag="PLZ54_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOJ67058.1"
                     /translation="MDYLEVNFTLSPYEEFIADLLMETLGEIGFESFQLADQQFFAYI
                     QESNFNQKILDDKLSKFEYPVSIDYNILLLENKDWNEEWEKNFFQPILIDNDCVVHSS
                     FHQKVPEAKYDIVINPKMAFGTGHHETTAMMLGELLKMNLGKKNLLDMGCGTAVLSIL
                     ASLRGASSVVAVDIDDWSIENSMENIRLNKIKNIELRKGGVEVLKDVHVDIVLANINR
                     NVLLKDMKFYAACLPIGGELYLSGFYVEDIPMIEAEAEKNGLQLAYFKEKNNWALVKT
                     IKK"
     gene            1447..2805
                     /locus_tag="PLZ54_08815"
     CDS             1447..2805
                     /locus_tag="PLZ54_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67059.1"
                     /translation="MKPAKLCILFLSVLLALPGYGQKNKASIDDVYFSWDDAKNTAKM
                     STESNKKPNTKNGAKEIIFIDRNEDAQNNSSRLLIAKANDSTTVDSDTLAADEGYYLR
                     DFNGSESDYEYAERIRRFHNPKFTIHISDPEYMDIYFLDDSQWNVYVGGSYAWITPTW
                     TNPAWFDYYYRPFSYNSWYWRSSLYYPYSFYGYHSWVYSPWYYDPWYYDPWFYYPAYY
                     RPYYGYPYYGYYGWSRPFYKSVDPSHNEAYRRQVANGLRNSNTNSSTRAGILSGVAGG
                     SSSTGRSGNPYTVVSGSGSRSSVSSDINSTGRRSSTSSYDNSRSSINSGRTTTGTRSQ
                     SEVTGTTGTRSTETYSGNSSRRTTETINTRPRTRSGNRGNSVATPQTGSRSSYTPSSG
                     SSERRSSYTPSSSDNSSRSSGSSYSGSSRSSGSSSGSSSGSSSGGGSSSSGSSRSSGG
                     RR"
BASE COUNT          898 a          556 c          507 g          859 t
ORIGIN      
        1 gaaacccacc tgtattgcat acacaaacac aaatacagac atgtgggttc catcagacct
       61 ttaaaaaata aattatattc tgatgaaagt taagaaagat aaggcttgat agccgagaaa
      121 tgaaaggcaa aaattcattt tagagcgaag atataaaggt ttgaatatag ttaaaacaat
      181 tattgctccg agaatcagac tgcccaaaag tgataaaaca ttgatcgaga tatgcaaatt
      241 actctaaaaa aattaatcgt tacccatttt tgatgatatg ttgtggaaaa tttttagggt
      301 gaagcattgt tgaagtcagg aatttatatt ctcaagcttt caatattaat aaatattttt
      361 atgtgtaaat agatgtttac ttttttattg tttttaccaa tgcccagttg tttttctctt
      421 tgaaatacgc caactgcaat ccattttttt cggcttctgc ctcaatcata ggaatatctt
      481 ccacataaaa tccgcttaga tagagttctc ctcctatcgg caaacaagct gcgtaaaatt
      541 tcatatcttt caacaaaaca tttctgttta tgttggcaag cactatatca acatgtacat
      601 ccttaagcac ttcgactcca ccttttctga gttctatgtt ttttatttta ttcaatcgaa
      661 tgttttccat tgaattttca atggaccagt cgtcaatgtc aaccgctacc actgatgaag
      721 ccccgcgcaa agaagccaga atggacaata ccgccgttcc acaacccatg tccaaaagat
      781 ttttctttcc cagattcatt ttcaacaact cacccagcat cattgcggtc gtttcatgat
      841 gacccgtacc aaaagccatt tttggattga taacgatatc gtattttgct tcgggaacct
      901 tctgatgaaa agaactgtga accacacaat cgttatcaat caatatcggc tgaaaaaaat
      961 ttttttccca ttcttcgttc cagtccttat tttccaacaa taatatattg taatcaatgg
     1021 aaacaggata ttcaaatttg gacaacttat catcgagtat tttctggttg aaattgcttt
     1081 cttgaatata agcaaaaaac tgttggtcag caagttgaaa gctttcaaat cctatttcac
     1141 ccaatgtctc catcaaaaga tcggcaataa actcttcata tggagacaat gtgaaattca
     1201 cttcaagata atccataata tttgacgcgt taaaatcgct aagaaaccat aaacgaatta
     1261 aaccttttca aaaaaagtaa atctaaaata taaaactcaa caaaattaca tatttttgtt
     1321 gagttcttca attgactaac tttttagtaa aaccgtaaaa ataagtatgc ttttttctta
     1381 caatggtaca attattgatg cttatataat tgcaaaatct atctacaaaa cacaggaggt
     1441 aatatcatga aacccgcaaa attatgtatt ttatttttat ctgttctcct tgccttgccg
     1501 ggatatggac agaaaaacaa agcatcgatc gacgatgtgt atttttcgtg ggatgatgca
     1561 aaaaataccg ccaagatgag tactgagtcc aataaaaagc ccaataccaa aaatggagca
     1621 aaagaaatta tttttatcga ccgaaatgaa gatgcacaga ataattcttc acgattattg
     1681 attgctaaag ccaacgacag tacaacagtt gactctgata cacttgctgc agatgaaggt
     1741 tattatttga gagattttaa tggaagcgaa tcggattacg aatatgctga aagaattcgc
     1801 cgttttcaca acccgaaatt taccattcat atttctgacc ccgaatacat ggatatttat
     1861 tttttggacg atagtcaatg gaatgtttat gtaggcgggt cttacgcatg gattactcct
     1921 acatggacca atcctgcatg gtttgattat tactatcgtc cgttcagtta taatagctgg
     1981 tattggcgaa gcagtcttta ttatccgtat tctttttacg gataccattc gtgggtctat
     2041 tcaccgtggt attatgatcc atggtattac gatccatggt tttattatcc cgcttattac
     2101 aggccttatt acggttatcc ttattatgga tattacggct ggagtcgacc tttttacaaa
     2161 tctgttgatc caagtcataa tgaagcctat cgccgtcagg ttgctaatgg tttgagaaat
     2221 tcgaatacca atagttctac aagagcaggc attctctccg gcgtagccgg cggaagctct
     2281 tcgactggcc gaagtggtaa tccttatacg gttgtaagtg gtagcggttc cagaagttct
     2341 gtttcgtccg acataaattc gactggccgc agaagttcaa cttcaagtta tgataattca
     2401 agatcttcta tcaattcggg cagaaccaca accggaacca gaagtcagag tgaagttacc
     2461 ggtacaacag gaactcgttc gacggaaact tatagtggaa attcttcgcg cagaaccaca
     2521 gaaacaatta atacccgacc gagaaccaga tccggaaata gaggaaattc ggttgcaacg
     2581 cctcaaaccg gttcacgaag ttcttacact ccatcttccg gttcttcaga aagaagatct
     2641 tcttacacgc ccagcagttc cgacaatagt tcgcgaagtt ccggcagttc ctattccggt
     2701 agctcgcgca gttcgggcag cagttcgggc agcagttcgg gcagcagttc cggcggagga
     2761 agttcaagta gtggatcgag ccgtagcagc ggaggaagga gataaaaaat cagaatttat
//
LOCUS       DAOSCH010000170         2716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_24255_length_2716_cov_6.489290, whole genome shotgun sequence.
ACCESSION   DAOSCH010000170 DAOSCH010000000
VERSION     DAOSCH010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24255_length_2716_cov_6.489290"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..2362
                     /gene="metG"
                     /locus_tag="PLZ54_08820"
     CDS             323..2362
                     /gene="metG"
                     /locus_tag="PLZ54_08820"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOJ67060.1"
                     /translation="MKQFKRTLVTSALPYANGPVHIGHLAGVYVPADIYVRYLRLKGE
                     EVIFIGGSDEHGVPITIQAKKEGVSPQEIVDRYHFLIKKSFEDFGISFDIYSRTTSKI
                     HHELASEIFKTINDKGGFIEKTSEQYFDEEAGQFLADRYIVGKCPHCGNENAYGDQCE
                     SCGSSLNPTDLIHPRSAITGNIPVLRETKHWYLPLDQHEEWLRHWILEEHKEWKPNVY
                     GQCKSWLDMGLQPRAVSRDLDWGIPVPLEGAEGKVLYVWFDAPIGYISNTKELLPDSW
                     EKWWKSEDTRLIHFIGKDNIVFHCIIFPTILKLEGTYILPDNVPANEFLNLEGDKIST
                     SRNWAVWLNEYLEDFPGKQDTLRYVLTANAPETKDNDFTWKDFQARNNNELVAIYGNF
                     VNRTLVLTQNYFGGKVPARNELQGIDIQAINELSEVKKNVEKLLDNFKFRDAQKEAMN
                     LARLGNKYLADTEPWKVAKTDKDRVATVLNISLQIAANLSIVFEPFLPFSSAKLRDLL
                     NLETFNWNNLGNIDLLKPQKELKKPELLFEKIDDRAIEYQIQKLQNTKKSNELSSFKA
                     NAVKSEVSFEEFEKLDIRVGTVLACQKVPKADKLLQFKIDDGLGGRTIVSGIAQYYPE
                     PEKLVGQQVCFIANLEPRKLKGIVSEGMLLSAEDADGSISLIQPQTKVVNGAEVK"
     gene            2566..>2716
                     /locus_tag="PLZ54_08825"
     CDS             2566..>2716
                     /locus_tag="PLZ54_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOJ67061.1"
                     /translation="MNLSDQLIDWYQVHKRDLPWRNISDPYQIWISEIILQQTRVNQG
                     LDYYLR"
BASE COUNT          888 a          438 c          563 g          827 t
ORIGIN      
        1 cttggggcac gaagttgcca gctttcctat gaaagaattt ttgcctgaaa ttgagaaatt
       61 tatcaaaatt tttgtatttg aaaaaaaact atggatggtt gaagtttatt ttacagacaa
      121 acaacgcaaa tctgaaataa acattgcacc aagtaattat tataaataat tataatgaat
      181 tcattatcaa ttattttatt gtgattgata tattcaattt taattttata aaaggataaa
      241 cgcgttagga aattcaggaa ttttgagttt ttgtcactat atttgcaaaa tattttgcaa
      301 aaagtgagat agaatttaaa atatgaagca atttaaacgt acgctggtta cttcagcttt
      361 gccttatgcc aatggtcctg ttcatatcgg acatttggca ggtgtttatg tgccggctga
      421 tatttatgtg agatatctcc gtttgaaagg agaagaagtg atttttattg gcggttcgga
      481 tgaacatggc gttcctatta ctatacaagc aaaaaaagaa ggtgtttctc cacaggaaat
      541 cgtggatcgt tatcattttc tgataaaaaa atcattcgag gattttggaa tttctttcga
      601 tatttattcc cgcaccactt caaaaattca tcatgaactg gcttcagaaa tttttaaaac
      661 cataaatgac aaagggggtt tcatagaaaa aacgagcgaa cagtatttcg atgaagaagc
      721 gggtcaattc cttgccgacc gatatattgt gggaaaatgt ccccattgcg gaaacgaaaa
      781 tgcttacggt gatcagtgtg aatcttgcgg ttcttcgctc aatcctactg atttgattca
      841 tccccgctca gcaattacag gaaatattcc ggttttgcgt gagaccaaac actggtattt
      901 gcctctcgat cagcacgaag aatggttgcg acattggata ctggaagaac acaaagagtg
      961 gaaaccaaat gtttatggac aatgcaaaag ctggctcgac atgggtttgc aaccacgtgc
     1021 cgtaagtcgc gatttggatt ggggcattcc tgttccactc gaaggcgccg aaggaaaagt
     1081 attgtatgtg tggtttgatg ctcccattgg ctatatttcc aacaccaaag aacttttgcc
     1141 agacagttgg gaaaaatggt ggaaaagtga ggatacaagg ttaattcatt ttatcggaaa
     1201 agataatatt gtttttcact gtattatttt ccccacaatc ttaaaactgg aaggaactta
     1261 cattttaccg gataatgttc cggccaacga atttctaaac ctcgaaggcg acaaaatttc
     1321 aacttcccgc aattgggcag tttggttgaa cgaatatctc gaagattttc ccggaaaaca
     1381 ggatactctg cgttatgtgc tgacagctaa tgctcccgaa acgaaggata acgactttac
     1441 atggaaagat tttcaggcac gaaacaacaa tgaattggta gctatttacg gcaattttgt
     1501 aaatagaacc cttgtgctga cacaaaatta ttttggagga aaagttccgg cgcgaaatga
     1561 attgcaggga atagatatac aggccattaa cgaattgtcg gaagtgaaga aaaatgttga
     1621 aaaattgctc gataatttca agttccgtga tgctcagaaa gaagccatga atcttgcccg
     1681 gctgggaaat aaatatcttg ccgacacaga accgtggaaa gttgcaaaaa cagacaagga
     1741 cagagttgct actgttttga atataagttt acaaatagcg gctaatcttt ctatcgtttt
     1801 tgagcctttt ttgcctttca gctcggcaaa actaagagat ttattgaatt tggaaacttt
     1861 taactggaat aatctgggca acattgattt gctgaaacca caaaaggaat taaaaaaacc
     1921 ggaacttctt tttgaaaaaa ttgatgacag ggctatagaa tatcagattc aaaaattaca
     1981 aaatacaaaa aaaagtaatg aactttcgtc gtttaaagct aacgcggtaa aatcagaagt
     2041 ttcttttgaa gaattcgaaa aactcgatat tcgtgttggt acggtgttgg cttgtcaaaa
     2101 agtcccgaaa gcagacaaac tgcttcaatt taaaattgat gacggtttgg gcggtcgtac
     2161 cattgtttca ggtattgctc agtattaccc tgaaccagaa aaattagttg gacagcaggt
     2221 ttgttttatt gccaatcttg aaccacgcaa attaaaagga atagtcagtg aaggaatgct
     2281 actctcggca gaggatgctg atggcagcat atcattgatt cagcctcaaa caaaagtggt
     2341 aaatggagct gaagtgaagt gatgagtttc gagtgatgag tttcgagttc tgaatttaaa
     2401 gctttgaata ttttgaaaaa aaattcagaa atacagcaat aatgaaaaat gaaagaggtt
     2461 ttaggggaag gctatgtaaa tagcatttga tgtgaagtaa atttcaattt gggttgtttg
     2521 ttgttttaat atttcctcca attcgcaagt tttttgttct ttcatatgaa tttatccgat
     2581 caacttatcg actggtatca ggttcataaa cgtgatttgc catggcgaaa catttctgat
     2641 ccttaccaaa tttggatttc cgaaatcatt ttgcagcaaa cgcgtgtcaa tcaagggttg
     2701 gattattatt tgcggt
//
LOCUS       DAOSCH010000171         2708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_24376_length_2708_cov_4.368262, whole genome shotgun sequence.
ACCESSION   DAOSCH010000171 DAOSCH010000000
VERSION     DAOSCH010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24376_length_2708_cov_4.368262"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="PLZ54_08830"
     CDS             complement(<1..281)
                     /locus_tag="PLZ54_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOJ67062.1"
                     /translation="MKLKGKVAIVTGGARDLGRAVSIKLASEGAKVVVNYFDNLEDAK
                     ETLKMIQENGGEGIIVQGDMTKAEEVKKLFDAGIAAFGNRIDILVNVVGG"
     gene            complement(303..1766)
                     /locus_tag="PLZ54_08835"
     CDS             complement(303..1766)
                     /locus_tag="PLZ54_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOJ67063.1"
                     /translation="MKIKGLRWWIIGLIMVITIINYLDRGTLNYMWVTNVEYSIVDQF
                     STNTKNYEAVFLKEKNSYLLKNKKGETAEVISSHLLEKKDKKVVVNREGIAYDLGIID
                     KNTTPDEASKKAKGILGTITIFFMIAYGISQLFSGKMYDKIGTRKGFVISAAIWGMAD
                     ALTSLAKGKFSLTAFRMMLGLGEAGPWPGAVKSNAEWFPQKERAFAQGLFNAAASAGS
                     ILAPIIILMLYLLLGWRLTFLIVGSLGLIWIIPWLIINKKGPKDHPWITEKEREYILT
                     GQPEAQHVNEKGKSWKELLSNKKNYSVILGRFFLDPIWWMFVTYLPLYLVEQFNLNIT
                     ELAFSAWVPYFGAMIGSLTGGWFSGYLIRSGKTVDKARKIAMIVGGLIIIPSVIASVL
                     ASNAILAVVLMAFVLGGFQFTMTNIQTIPSDLHSGPSVGSLAGLGGAAAVLGTILAIL
                     FSAQITSWPLLFGLLAALVPLSLGSIFLTVGKIEHIK"
     gene            complement(1771..2115)
                     /locus_tag="PLZ54_08840"
     CDS             complement(1771..2115)
                     /locus_tag="PLZ54_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOJ67064.1"
                     /translation="MKTRSLNFLHASQIKPEITGEGISRQILGYDGQIMMVKVHFKKG
                     AVGYIHEHFHSQTTYVASGKFEVSINGEKQILEAGDGFYIEPDAPHGAICLEDGILID
                     VFSPMRMDFLKI"
     gene            complement(2127..>2708)
                     /locus_tag="PLZ54_08845"
     CDS             complement(2127..>2708)
                     /locus_tag="PLZ54_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67065.1"
                     /translation="HTLDFPFYYRGQMISTNIQYQKFTAQLNPLGTKNGYQHLWVEAI
                     GKNEKPVTQFTWVEGNRFYTITTLGNSNTEYLITRTGAGDPNFNLRNETAFMMRQPMV
                     KKHTFVSVVEPHGFYDENKEITENFETSIKNVELYADNDEYSAVKISTVENNTFLLIA
                     LNKDFNKDREHSIKIDNQTVEFRGNYYFTELTK"
BASE COUNT          799 a          564 c          430 g          915 t
ORIGIN      
        1 ccgccaacta cattgaccag tatgtcaatt ctatttccaa aagcagctat accggcatca
       61 aataattttt ttacttcctc tgcttttgtc atatcgcctt gaacgataat accttcacca
      121 ccattttcct gtatcatttt caaagtttct ttagcatctt cgagattgtc aaaataattg
      181 acaacaactt ttgctccttc agaagccaat ttaattgata ctgctcttcc taaatcccga
      241 gctccaccag ttacgatggc aacttttcct tttaatttca taattataat atttttaatt
      301 gattatttta tgtgttctat ttttcctacc gttaaaaata ttgatcctaa cgacaacggt
      361 accaacgccg ccagcaaacc aaaaagtaat ggccaactgg taatttgtgc agaaaataaa
      421 atggccaaaa tggttccgag tacggctgct gctccaccca atcctgctaa agaaccaact
      481 gatggcccac tgtgtaaatc acttggaata gtttgaatgt tggtcattgt aaattggaag
      541 ccacccaata caaatgccat taaaacgact gccaatattg cattggaagc caatacagaa
      601 gctattacgg aaggaataat tattaatcct ccaactatca ttgctatttt tctggccttg
      661 tcaacggttt ttccgctgcg gattaggtaa cctgagaacc aaccaccggt tagacttcct
      721 atcatggctc caaaataggg cacccatgca gaaaaagcca gttccgtgat attcagattg
      781 aattgttcaa ccaaataaag tggaagatac gttacaaaca tccaccagat gggatcgaga
      841 aaaaaacgac ctaaaataac ggaataattc tttttatttg aaagtaattc tttccaactt
      901 ttcccttttt cgtttacgtg ttgagcttcg ggctggccgg taagaatgta ttcacgttct
      961 ttttccgtaa tccagggatg atcttttggt ccttttttgt tgattatcag ccagggaata
     1021 atccagataa gtcctaaaga accaacaatc aggaaagtaa gtctccatcc cagcaacaag
     1081 taaagcatta aaattatgat aggagcaagg attgaaccgg ctgaagctgc tgcattaaaa
     1141 agtccctgtg caaaagctct ttctttttga gggaaccact cggcattgct ttttactgcc
     1201 ccgggccacg ggccagcttc ccccaatcca agcatcattc tgaaagcggt aagtgaaaat
     1261 tttccttttg ccaaagaagt tagcgcatct gccattcccc agatagcagc tgatatgaca
     1321 aagccctttc gcgtaccaat tttgtcgtac attttacccg aaaacaactg gctaatacca
     1381 taggcaatca taaaaaatat ggtaatagtt cctaaaattc ctttagcttt ctttgatgct
     1441 tcatcaggtg tggtattttt atcgatgatg cctaaatcat aagctatccc ttctctgttc
     1501 accactactt ttttatcttt tttctccaat aagtgagatg aaatcacttc tgctgtttct
     1561 ccctttttgt ttttcagcaa ataactgttt ttttctttca aaaaaacggc ttcgtaattt
     1621 ttagtgttgg ttgaaaactg atcaactatg gaatattcga cattcgttac ccacatgtaa
     1681 ttcaatgtac ctctatccag atagttaatg atggttatga ccataattaa tccaataatc
     1741 caccaacgca atcctttgat tttcatatat ttatattttt aagaaatcca tcctcattgg
     1801 gctgaaaaca tcgatcaaaa ttccatcttc cagacatatt gctccgtgcg gagcgtcggg
     1861 ttcgatataa aagccgtctc cagcctccaa aatctgtttt tcgccattta tcgaaacttc
     1921 aaatttgcca ctggctacat aagtagtttg tgaatgaaag tgttcatgaa tataaccgac
     1981 cgctcctttt ttaaaatgga cttttaccat catgatttga ccatcgtacc ccaaaatttg
     2041 tcttgaaata ccttcaccag tgatttccgg tttaatttgc gacgcatgta aaaaatttaa
     2101 acttcttgtt ttcatatctg tgtaatttat tttgttaatt ctgtgaagta ataattgcct
     2161 ctgaattcaa ctgtttggtt atctattttt attgaatgct ccctgtcttt attaaaatct
     2221 ttattcagcg caatcagaag aaatgtattg ttttcaacag ttgatatttt tactgccgaa
     2281 tattcgtcat tatcagcata aagttcaaca ttttttattg aggtttcaaa attttctgta
     2341 atttccttgt tttcatcata aaacccatgt ggctcaacta ctgagacaaa tgtgtgtttt
     2401 tttaccatag gttggcgcat cataaatgca gtttcatttc tgagattaaa atttgggtca
     2461 ccagctcctg tacgtgtaat cagatattct gtgtttgaat tacctaatgt cgtaatcgta
     2521 taaaatctat ttccttcaac ccacgtaaac tgagtaaccg gtttttcgtt tttcccgatt
     2581 gcctcaaccc aaagatgttg ataaccattt ttcgttccca atggatttaa ttgagcagta
     2641 aatttttgat attgaatatt ggtagaaatc atttgacctc tatagtaaaa agggaaatca
     2701 agtgtatg
//
LOCUS       DAOSCH010000172         2641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_25241_length_2641_cov_7.021268, whole genome shotgun sequence.
ACCESSION   DAOSCH010000172 DAOSCH010000000
VERSION     DAOSCH010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25241_length_2641_cov_7.021268"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1488
                     /gene="pepT"
                     /locus_tag="PLZ54_08850"
     CDS             262..1488
                     /gene="pepT"
                     /locus_tag="PLZ54_08850"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HOJ67066.1"
                     /translation="MTLTDRFLRYVSFTTTSDELTNMTPSTPGQMVFAQHLVKELKSF
                     GLKDAEVDKNGYVMATLPANTDKKIPTIGFIAHLDTSPDMSGKNVKPQIIENYDGKDI
                     LLNEEQLIVLETEKFPEILQYVGQDIIVTDGTTLLGADDKAGIAEIISAVEYLLRHPE
                     IKHGKIRIAFTPDEEIGKGADNFDVKKFNADWAYTIDGGEIGELEFENFNAASAKITF
                     HGLNVHPGYAFHKMKNSIRIAHQFVAMLPRHETPEHTEGYEGFYHLTSIEGTVEKTTM
                     HLIIRDHDRDKFEKRKKELQHLANKINAEFGENTAVIELRDQYYNMREKIEPVFHIID
                     LASDAMKEVGITPKIVPVRGGTDGARLSYMGLPCPNIFAGGMNFHGRFEYIPIQSMEK
                     AMMVIVKIVELAAKKY"
     gene            2107..2538
                     /gene="dut"
                     /locus_tag="PLZ54_08855"
     CDS             2107..2538
                     /gene="dut"
                     /locus_tag="PLZ54_08855"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOJ67067.1"
                     /translation="MKIKIVNLSKHPLPSYATLLSAGMDLRANIDEPVVLKPLERKLI
                     PTGLFIELPEGYEAQIRPRSGLAIKHGVTVLNSPGTIDADYRGEICIILINLSNDDFV
                     INDGERICQMVIASHEHAEWEEVKMLNDTDRGNGGFGHTGR"
BASE COUNT          918 a          474 c          497 g          752 t
ORIGIN      
        1 gcaggtagag cacatccctt ttaaggatgg ggtcctgggt tcgaatccca gctggatcac
       61 ttcttttttc aacagattta tcatctttcc cgcttgcttc ctacaaaaaa gttgcattaa
      121 tttcttgaat ttaaatccaa ctaaatactc tctttgaaac aactatcgga atacgttttt
      181 tcgcacataa agaaaaaatt atccggaaat agtttcttaa taaaaaatct gtaactttga
      241 agcaataaaa aatcgacttt catgacatta accgacagat ttttaaggta cgtaagtttt
      301 acaacaactt ccgacgaatt aaccaacatg acacccagta cgccgggtca aatggttttt
      361 gctcaacatt tggtaaaaga attgaagtct tttgggttga aagacgccga agtggataaa
      421 aacggctatg taatggcaac tttgccggcc aataccgata agaaaattcc aactatcggc
      481 tttattgccc atttggatac cagccccgat atgagtggaa aaaacgtcaa acctcagatt
      541 attgaaaatt acgatggcaa agatatttta ctgaacgaag aacagctaat tgtgctggaa
      601 acagaaaaat ttcctgaaat tttgcagtat gtgggtcaag atattattgt taccgatggc
      661 actacattgc ttggtgccga tgacaaagca ggcattgctg aaattatttc tgctgttgaa
      721 tatcttctgc gtcatccgga aattaaacat ggaaaaattc gcattgcttt cactcctgat
      781 gaagaaatag gaaaaggagc agataatttc gatgtgaaga aattcaatgc cgattgggca
      841 tatactattg atggtggaga aattggagaa ttggaatttg agaatttcaa tgctgcctca
      901 gccaaaataa cttttcacgg attaaatgtt catccgggct atgcatttca taaaatgaaa
      961 aattccattc gtattgctca tcagtttgta gccatgcttc cccgccacga aacgccggaa
     1021 cataccgaag gttacgaagg tttttatcat ttaacgtcca tagaaggtac ggttgaaaaa
     1081 acgaccatgc atcttatcat tcgcgaccac gaccgcgaca aatttgaaaa aaggaaaaaa
     1141 gagcttcagc atttggctaa taaaatcaat gcagaatttg gagaaaatac cgctgttatc
     1201 gaattacgag atcaatacta caacatgcgt gaaaaaattg aaccggtttt tcacattatc
     1261 gatttggcat ccgatgccat gaaggaagtg ggcattacgc ctaaaattgt tccggtaaga
     1321 gggggaacag atggcgcccg actttcgtat atgggacttc cctgtccgaa tatttttgcc
     1381 ggagggatga attttcatgg gcgtttcgaa tacattccca ttcaatctat ggaaaaagct
     1441 atgatggtga ttgtaaaaat tgttgagttg gctgcaaaaa aatactaaaa tttctctgac
     1501 aaaaaatagt ggcctgttca gtaataatga atgattttgc aaatgaattt gataatcgat
     1561 tgattacatc tcggacttca tacaaggact ctactctttc cattttcctt tttgacaatt
     1621 tttggatagc aagcatacag atgcttgttt aaaagcgcat tcacgagtat ttggaacatt
     1681 ttttgaagtg taacaaaaag taaaaagaaa atgtaagcct ttacggattt ctgaataatt
     1741 aaccattttt tcagcaggag gaaacaacga aagaaaaatc tataaaagga acattacaaa
     1801 aaacttggaa aatactttag aatagagaat aaggaaccaa aattaaaaca atagaaatat
     1861 taactgcatt aaagatatta aagcgaaaaa gggaaataaa acagggttac aaatcaaaca
     1921 aataccaatc aactatcttt gaggtattaa cgctttatcc ggattcataa aattccggta
     1981 ttgttttatt cgatttaaaa acatttactg atgaaatcca catgtattgc ctacacaaac
     2041 acgaatgaat ataatatgtc agacatgtgg gttccatcag acctttaaaa aataaattat
     2101 attctgatga aaataaaaat tgtcaacctt tcgaaacatc cactgccatc ctacgccact
     2161 ttactttctg ccggaatgga tttgcgagcc aatatcgacg agccggtagt tttaaaacct
     2221 ttggaaagga aactgattcc cacaggactt tttatcgagt tgccggaagg atatgaagca
     2281 caaatacgcc cgcgaagcgg actggccatc aaacacggcg ttacggtgct caattctccg
     2341 ggcactattg atgccgacta tcgcggcgaa atttgtatta ttctgatcaa tctttccaat
     2401 gacgattttg taatcaacga tggcgaacga atatgccaaa tggtgattgc ttcgcatgaa
     2461 catgcagaat gggaagaagt aaaaatgttg aacgataccg atcgaggaaa tggcggattt
     2521 ggacatacgg gacgataaat tccaaaaaat gtagaaattc tgaatcgaat ctactattat
     2581 cggaaaataa caagcaaaac aatttttcat gcacaaaaaa atttttttat ttcccatatt
     2641 a
//
LOCUS       DAOSCH010000173         2634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_25335_length_2634_cov_5.848391, whole genome shotgun sequence.
ACCESSION   DAOSCH010000173 DAOSCH010000000
VERSION     DAOSCH010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25335_length_2634_cov_5.848391"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="PLZ54_08860"
     CDS             complement(<1..1434)
                     /locus_tag="PLZ54_08860"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOJ67068.1"
                     /translation="MNFIKRYGLLIFLAIFAVSSPSQNLSGIKICVNPGHGGYDSDDR
                     NVVIAPFTSGDPNGFWESKSNLDKGLMLKSMLENAGATVIITRTTNTTADDLPLSQIV
                     AMANSANADYMLSIHSNAGNGIANYVLQLYAGLDPNDTYTYPTPTPFSDESRAISTIV
                     AQNLYSNQITNWSATYAVRGDKTFARTAMGWSDGYGVLRGLAVPGAISEGSMHDYIPE
                     TYRLMNMEYKWLEAWHFFKSFCQYYQAGNIPTGNIAGSVRDSRNIIQESYYKLKDTRD
                     ELLPLNGATLTLLETNETYTTDQLHNGVYMFKNLAPGVYHVKAEANGYHAQIFETTVT
                     ANNTTYLNIDLNKVRNTPPQVISYSPNIAPPDSAECSTDIVLTFNWDMDVTSTTEAFS
                     ITPSVEGTITFEDSQRILRFKPLLPLEKATLYTVRLAKTAKHPDNLSMTEDFTFQFLT
                     KNRNRLKLLAAYPYEGNHKVYTKPQFRF"
     gene            complement(1449..2291)
                     /locus_tag="PLZ54_08865"
     CDS             complement(1449..2291)
                     /locus_tag="PLZ54_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67069.1"
                     /translation="MKRYKVNGNDIKGSFPVLSPAGDKLLFSSENHKGLWLYDLNSKE
                     LITICENEGAGYAPIFNADGTKVFYRKTIYQDKRKFDAIESFDLVKKQKVEMISPQRN
                     LSLLQNYHNGFLVSTDRKLLKATFGKTMNNIPPYVCTEDLKLVVYANGKRTELNPIGE
                     NDARYIWVSLSPDSEKILFTVAGKGTFVSDLEGKIVASLGYLNAPVWYDNNFVVGMQD
                     KDDGHFITSSKVLMVSLNGKTKMTLSLDSEIAMYPSASSKGNKVAYNKLEGDIIIVEL
                     NVKP"
     gene            complement(2447..>2634)
                     /locus_tag="PLZ54_08870"
     CDS             complement(2447..>2634)
                     /locus_tag="PLZ54_08870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ67070.1"
                     /translation="NNDAKLLQAQLFHLNGQCVVSQQLNPMNGIATFKIGKLPAGTYL
                     LKIIHKETSETINLLIK"
BASE COUNT          775 a          472 c          498 g          889 t
ORIGIN      
        1 gaatctgaat tgaggttttg tataaacttt atgattacct tcatagggat aagcagcaag
       61 taattttaaa cgatttctat ttttggttaa aaactggaaa gtaaaatctt cagtcatgct
      121 taaattgtcg gggtgtttag cagtttttgc caatcgaaca gtgtaaagcg ttgctttttc
      181 aagcgggagc aatggtttga atcgcaaaat gcgctgactg tcttcaaaag tgatggttcc
      241 ttctactgac ggcgtaatcg aaaaagcttc tgttgttgaa gttacatcca tatcccaatt
      301 gaaagtcaaa acaatatcgg tagaacattc ggcactgtcg ggaggagcaa tattaggaga
      361 gtaagaaatt acttgtggtg gagtatttct tactttattc aaatctatat tcaaatatgt
      421 ggtattattt gctgtaactg tagtttcaaa tatttgagca tgataaccat tggcttccgc
      481 ttttacatga taaactcccg gagcgagatt tttaaacata taaactccat tgtgaagttg
      541 atctgttgta taagtttcat ttgtttccaa aagtgttaat gttgcaccat ttagaggaag
      601 caactcatct ctggtgtctt tcagcttata atagctttcc tgaattatat ttcgactgtc
      661 gcgaacggag ccggcaatgt ttcctgtcgg gatgttccct gcctgatagt actgacaaaa
      721 ggatttaaag aaatgccagg cttcaagcca tttatattcc atattcataa gcctataggt
      781 ttcaggaata tagtcatgca tagatccttc agaaatagct cctggaacag ctaatccacg
      841 cagcacacca tatccgtcgc tccaacccat agctgtgcgt gcaaatgttt tatcaccacg
      901 cactgcataa gttgccgacc agttggttat ctggtttgag taaagatttt gtgcaacaat
      961 tgtgctgatg gctctgcttt catccgaaaa aggtgtgggt gtaggatagg tataggtatc
     1021 gttaggatcg aggcccgcat aaagttgtag aacatagttg gcaataccat tccctgcatt
     1081 gctgtggata gaaagcatgt agtctgcatt tgccgaattt gccatagcta caatttgaga
     1141 taatggtaaa tcgtctgctg tagtattggt ggttcgggta ataataaccg tcgcaccagc
     1201 attttccaac attgatttaa gcattaatcc tttgtccaaa ttgcttttcg attcccaaaa
     1261 tccgttcgga tcgcctgaag tgaaaggagc aataactaca tttctgtcat cgctgtcgta
     1321 accgccatga ccgggattaa cacatatttt tatacctgat aaattttggg atggggaaga
     1381 aacggcaaaa atcgctaaaa aaattagcaa gccatacctt ttgataaaat tcatgatttt
     1441 ttactgattt atggttttac attcaattcc actattatga tatctccttc caatttattg
     1501 taggcaactt tatttccttt agaagaagct gaaggataca tggcaatttc gctatcgagg
     1561 ctcaaggtca ttttagtttt accgttcagc gaaaccatta gcactttcga ggaagttata
     1621 aaatgtccat catctttgtc ctgcatgccc actacaaagt tattgtcata ccaaaccggc
     1681 gcattcaggt atccgagcga agcaacgatt tttccttcca aatcggatac aaaagtccct
     1741 tttcctgcga cggtaaagag aattttttcg ctatcgggag agagtgaaac ccaaatgtat
     1801 ctggcatcgt tttctccaat cggatttaat tccgttcttt ttccattggc ataaactacc
     1861 agtttcaagt cttccgtaca aacataggga ggtatattat tcattgtttt accaaaagta
     1921 gctttcaata gttttcggtc ggtagatacc aaaaatccgt tgtgataatt ttgcagaaga
     1981 gacaaatttc tttgtggtga aatcatttcg actttttgtt tttttactaa atcaaaactt
     2041 tcgatggcat caaattttcg tttgtcctga taaatagtct ttctgtaaaa aacttttgta
     2101 ccatcagcat taaatatcgg agcataacct gctccttcat tttcacaaat agtaatcaat
     2161 tctttagagt ttaagtcgta cagccacaat cctttgtgat tttccgatga gaacaagagc
     2221 ttatctccgg cagggcttaa taccggaaac gaacctttga tgtcgtttcc attcactttg
     2281 tagcgcttca caatgtttac attttgagca gataatgagt ttattatcaa aaatagcccc
     2341 agtaaacaga ttttgaatgt tttcattgga ttctgtttta aaatgattat aatagtgaat
     2401 tgaaatattt gaaattgtta aatttattat gatagtgaat aagattctat ttgatcagaa
     2461 gatttattgt ttctgaagtt tctttatgaa taattttcaa caaataagtt ccagcgggta
     2521 atttgccaat tttaaaagta gctattccat tcattggatt tagttgttga gaaaccacgc
     2581 attgaccatt taaatgaaag agttgagctt gcagaagttt tgcgtcatta tttt
//
LOCUS       DAOSCH010000174         2594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_25908_length_2594_cov_4.426546, whole genome shotgun sequence.
ACCESSION   DAOSCH010000174 DAOSCH010000000
VERSION     DAOSCH010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25908_length_2594_cov_4.426546"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..1755
                     /locus_tag="PLZ54_08875"
     CDS             238..1755
                     /locus_tag="PLZ54_08875"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOJ67071.1"
                     /translation="MIGLADISTNSKILEPSCGEGVFLKKLQEKGYYNLTAFEIDPNL
                     ATNFPFVRYESFVSASIDEKFDLIIGNPPYIRWKNLEEELKQELSSNPLWNKYFNSLC
                     DYLYIFILKSIELLNENGQLIFICPEYWMNTTHSISLRNYMVQNGYFEEIYHFNETPI
                     FDKVTVSIVIFKFVKSKQKTDKIKVAKFYANQKLTSETLANLKNKIQFKNAEYLQISQ
                     FKINERWLLQSDEVRKELQQLEIACKKQNQTSLLIFFDDEKTEFHTIGEICDIGNGLV
                     SGLDKAFQLNGLKLNSAEESATIKVVKAKDLKPFFAEHITKYIFINEGFEESEFQKMY
                     PNFYAHFQKYKTDLEKRYQYNRKINYWEWVFLRNFNLFSKAEKRIFVPCKERISNKNY
                     FRFALVEGGVFPTQDVTAIFRKPETKESIEYILAYLNQPIIFDWLKCNGIVKGNIIEF
                     SEKPIASIPFRKINWYDKNEILLHDSITNLTREYLNCKNKIILDEINKLFNQLIK"
     gene            1759..2526
                     /locus_tag="PLZ54_08880"
     CDS             1759..2526
                     /locus_tag="PLZ54_08880"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HincII family type II restriction endonuclease"
                     /protein_id="HOJ67072.1"
                     /translation="MFVNYTKLATKIKGTSVPKPISGTLSGHAAGEPFDKHVYNEIKK
                     QFPKNTFRQYEYLNDLFSKNPEVIGYEARQALFNSPTVLFLLSRGKNAVDKWSIENPF
                     DEKQNDTADILVVKDNFYEIIDIKTRNISKSAQPPNIISAFKLAQVCAKMLDNKEFDN
                     FTINYFEIDWLLDNDKLVCKDTHFACLFKAQPNELYINWAAAMQIQFHVRDLDQTYNG
                     TMENWAKSYLKHFVTQAKKRADDMIKKFVKPFEKYIE"
BASE COUNT          993 a          389 c          428 g          784 t
ORIGIN      
        1 tatacattca aaattggata tatagcgata taatgtagcg tatataaacg agttgtgtgc
       61 cattttaaat caaaagtatg tcaattgaat cttcggaata aaaatataac ttacagaaac
      121 caagaccaac actaaagttg gtattgcaaa agaaacattc aaatgaaagt agcagtaaaa
      181 cagagtagca aaaacaaatt cggacaatac tttacacccg aagtagtggc agaatttatg
      241 attggtttgg cggatatttc tacaaactca aaaattttag agccatcttg tggcgaaggc
      301 gtttttttaa aaaagttgca agaaaaaggc tattacaact tgactgcttt tgaaattgac
      361 ccaaacttag ctactaactt ccctttcgtt cgttatgaga gttttgtttc tgcgagcatt
      421 gatgaaaaat ttgatttaat aatcggaaac ccgccatata tacgttggaa aaatttagaa
      481 gaggaattaa agcaagaact ttcaagtaat ccgctttgga ataaatattt taattcgctt
      541 tgcgactatc tgtatatttt cattttaaaa tctattgaat tattgaacga aaacggacaa
      601 ctgattttca tttgtcccga atattggatg aatacaactc attccatttc gttgcggaac
      661 tatatggtgc aaaatggcta ctttgaagaa atttatcact tcaacgaaac accgattttt
      721 gacaaagtaa cagtttctat tgttattttt aaatttgtaa aaagcaaaca aaaaaccgac
      781 aaaataaaag ttgcgaaatt ttatgccaat caaaaattga cttctgaaac acttgcaaac
      841 ttgaaaaata aaattcagtt caaaaatgca gaatatttac aaatatcaca gttcaaaatc
      901 aatgaacgtt ggcttttgca aagtgatgaa gttagaaaag aattgcaaca attagaaata
      961 gcttgcaaaa agcaaaatca gacttcgttg cttatttttt ttgacgatga aaaaacagaa
     1021 tttcacacaa ttggcgaaat ctgcgacatc ggaaatggac ttgtaagtgg tttggacaaa
     1081 gcgtttcaat taaatggact aaaattaaat agtgctgaag aatcagcaac tattaaggta
     1141 gttaaagcaa aagatttgaa accgtttttt gctgaacata ttacaaaata tattttcatc
     1201 aatgaaggct ttgaagaaag tgagtttcaa aaaatgtatc ctaattttta tgcgcatttt
     1261 cagaagtata aaactgattt agaaaaacgt tatcaataca accgaaaaat aaattattgg
     1321 gaatgggttt ttttaaggaa tttcaatcta ttcagcaagg ctgaaaaacg aatttttgtt
     1381 ccttgcaaag aacgaatttc caacaaaaat tattttcgtt ttgcgttagt tgaaggtggt
     1441 gttttcccta cacaagacgt aactgctatt tttagaaagc ctgaaacaaa agaaagcatt
     1501 gaatatattt tggcttatct caatcagcca ataatttttg attggctaaa atgtaatgga
     1561 attgtaaaag gaaatattat tgaattttca gaaaaaccaa tagcaagcat tccgttcaga
     1621 aaaataaatt ggtatgacaa aaatgaaatt ttactacacg attctattac caatttaact
     1681 cgagaatatc ttaattgtaa aaataaaata attttagatg aaatcaataa attgttcaat
     1741 caacttataa aataaattat gtttgtaaat tacactaagt tagcaacaaa aattaaaggt
     1801 acttccgttc ctaaaccaat ttctggcacg ttgtccggtc atgcggcagg tgaaccgttt
     1861 gacaaacacg tttacaacga aatcaaaaag caatttccaa aaaatacatt tcgtcaatac
     1921 gagtatttaa acgatttgtt tagcaaaaat cccgaagtaa ttggctatga agcaagacaa
     1981 gcattattca attcaccaac tgttctgttt ttgttaagtc gaggtaaaaa cgcagttgat
     2041 aaatggagta ttgaaaatcc ttttgacgaa aaacaaaatg acacagctga cattctagta
     2101 gtcaaagaca atttttacga aatcatagac atcaaaactc gtaatatttc taaatctgca
     2161 caaccaccaa acattatctc agcgttcaaa ttagcacagg tttgtgccaa aatgcttgac
     2221 aacaaagagt tcgataattt cacgataaac tattttgaaa ttgattggtt gttggataac
     2281 gataaattag tatgcaagga cacacatttt gcttgtttgt ttaaagcaca accaaacgaa
     2341 ctttacataa attgggcagc tgcaatgcaa attcagtttc acgttcggga cttggaccaa
     2401 acatacaatg gaactatgga aaattgggca aagtcatatt tgaaacattt tgtaacacaa
     2461 gcaaaaaaac gagcggacga tatgattaag aaatttgtaa aaccatttga aaaatacatt
     2521 gaataaaaaa aacggcacac aacagcggtt tggcgtaatg gcgggtgcag tgcttcgtat
     2581 gacagttttg tggt
//
LOCUS       DAOSCH010000175         2586 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_26000_length_2586_cov_7.722639, whole genome shotgun sequence.
ACCESSION   DAOSCH010000175 DAOSCH010000000
VERSION     DAOSCH010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26000_length_2586_cov_7.722639"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            15..599
                     /locus_tag="PLZ54_08885"
     CDS             15..599
                     /locus_tag="PLZ54_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOJ67073.1"
                     /translation="MKKNTIIIVIAALALFFLWGVSRYNKLVSAEEAVSSQWGNVENQ
                     YQRRADLIPNLVATVKGYAAHESQTLEAVTAARAKATQVTVDPASLTPEKLQEFQAAQ
                     GELSTALGRLLMITENYPDLKANQNFIELQAQLEGTENRIAVERNRFNEIAKNYNATI
                     RRFPTSLIAAMFGFEKKPYFEAQEGSEKAPEVKF"
     gene            complement(784..>2586)
                     /locus_tag="PLZ54_08890"
     CDS             complement(784..>2586)
                     /locus_tag="PLZ54_08890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOJ67074.1"
                     /translation="EDRLLTATDIAAALSTVDMNALERIEVIKGAGSVLYGTGAMGGV
                     VNFVIERPAFTPFLESQGNIHSSFNSSNQLWSNHAKLQLTNRQWYLSLNGSYRTADNM
                     RTPEGKIPNSQFNDASWGMQAGMSSVANQELLVNYQHFEAWNVGLPGGSAFPPTATVR
                     YKNVQRNLFDAEYVFYEITDYLKQLRLKAYTQNISRDVENIVNTSTAIFPSSNNNTSG
                     IKAVGDWEFTAYHKLTLGTEGWLRDAKTSRIKKVNSGTQINVFGEQPTPNAQVQDMGI
                     FAQYSWEFSPRYWILDAGIRLDYMKTTNDSAFQPVYKYSYFPENPSMRIYEDIPRSLL
                     FKADEKNEFSYSAHIDLIYHPTRRQMLALSVSNSYRAPSLEELFKYIDQAGTLRVGNP
                     DLKPEKGFFSNFSYTLSEDHFYIKTDLFANYLFDLIAEQHGTYTYQDEKGKVTQREAL
                     INTNIDKALFLGGEMELQWKINPVFSVLANLSYTQGRNIDKDTFLPQIPPMHGLFSVN
                     YHQKELFDASVSALWAAKQGKVAPGENPTNGHLIFNADLHTEKFRINRNYVRFFAGAE
                     NIFDKAYYNHLSTTRGVLRLEPGRNIYVKAELGW"
BASE COUNT          787 a          510 c          507 g          782 t
ORIGIN      
        1 taaataaaaa aattatgaaa aaaaacacca ttatcattgt tatcgccgct ctggcactgt
       61 tttttttatg gggagtttcc cgttacaaca aattggtttc ggcggaagaa gctgtttcga
      121 gccaatgggg aaatgttgaa aatcaatatc aacgacgtgc cgatttaatt ccaaatctgg
      181 tagcaaccgt caaagggtat gctgcacacg aaagtcaaac tttggaagcc gtaacggcag
      241 ctcgtgccaa agccactcaa gttactgtgg atccagcctc gcttacgccg gaaaaattgc
      301 aggaatttca ggctgctcag ggagaattga gcaccgcact gggaagattg ctgatgatta
      361 ccgaaaatta tcctgatttg aaagccaatc agaatttcat agaattgcaa gcacaactgg
      421 aagggacaga aaatcgaatt gcagtggaac gtaatcgttt taacgaaata gcaaaaaact
      481 ataatgctac cattcgacgt tttccaactt ccctcatagc agcaatgttc ggtttcgaga
      541 aaaagccata ttttgaagca caggaaggaa gcgaaaaagc tccggaagtg aagttttaat
      601 agagaatttt tttgaactga tgaaaagcaa agaaaaatct tttctctgat tttcgaaaat
      661 aataaagacc aaaattaaaa ttcaaacaag aggagtaaaa agtcagcttt cgatgatttt
      721 tttactcctc ttttatctat ttttctgtga aataatgaaa taattcgttc agaaaaaact
      781 cttttaccaa cccaattcgg cctttacata gatatttctt cccggttcca gacgtaatac
      841 tccacgtgtt gtggaaagat gattgtagta ggctttatca aaaatattct ctgcaccggc
      901 aaaaaaacga acataatttc tgttgattct gaatttttct gtatgtaaat cggcattaaa
      961 aattaaatgg ccattcgtcg gattttcacc cggagcaact tttccttgtt ttgctgccca
     1021 caaagctgaa acagaggcat caaaaagttc tttttgatgg tagttcaccg aaaacaatcc
     1081 atgcataggt ggtatttgtg gaagaaaagt atccttgtca atatttcttc cctgtgtgta
     1141 ggacaaatta gccaacacgg aaaacacagg atttattttc cattgcagtt ccatttctcc
     1201 tcctaaaaag agtgccttgt cgatatttgt attgatcagt gcctctcttt gcgtaacttt
     1261 tcccttctcg tcctgataag tgtaagttcc atgctgctcg gcaatcaaat caaagaggta
     1321 gttggcaaaa agatcggttt ttatataaaa atgatcttcc gacaaagtat agctgaagtt
     1381 tgaaaaaaag cctttttcgg gctttaggtc aggattccca acacgcaagg ttccggcttg
     1441 atcgatatat ttgaataatt cttccaaaga aggcgcccta taggaattgg aaacagaaag
     1501 tgccaacatt tgacgacgtg tgggatgata aatcaaatcg atatgggcag aataggaaaa
     1561 ttcatttttt tcatcggctt tgaaaagcaa acttctggga atatcttcat aaattcgcat
     1621 ggatggattt tcaggaaaat aagaatattt gtacaccggc tggaaagcgc tgtcgttggt
     1681 ggttttcatg taatccaacc ttattccggc atccaatatc cagtaacgtg gcgaaaattc
     1741 ccaactgtat tgtgcaaaaa ttcccatatc ttgaacttgt gcattgggtg tgggttgttc
     1801 tccaaaaacg tttatttgtg ttcctgaatt aacttttttg atgcgtgatg ttttggcgtc
     1861 cctcaaccat ccttcagttc ctaatgtcag tttgtggtat gcagtaaatt cccagtcgcc
     1921 cacagctttg atgcctgaag tattattatt gctggaagga aaaatcgctg tagatgtatt
     1981 gacaatattt tctacgtctc tgctgatatt ttgcgtataa gctttaaggc gaagttgttt
     2041 taaataatcg gttatttcgt aaaaaacata ttctgcatca aataaatttc tttgtacgtt
     2101 tttgtatcga actgttgccg ttggtggaaa agcgctaccg ccaggcaatc cgacattcca
     2161 tgcttcaaaa tgctgataat taaccaataa ttcctgattg gcgaccgacg acatgccggc
     2221 ttgcattccc caggaagcat cgttaaactg actgtttgga atttttcctt caggtgtacg
     2281 catattatcc gcagtacgat aacttccgtt caacgacaaa taccactggc gattggttaa
     2341 ctgcaatttt gcgtggttgc tccaaagttg attggaagaa ttaaaggacg aatgaatatt
     2401 tccctgactt tccaaaaaag gagtaaatgc tggacgctca atcacaaaat tcaccactcc
     2461 gcccatagct cccgtgccgt ataaaacaga gccggctcct ttgatcactt ctattctttc
     2521 caaagcattc atatccactg tggacaatgc tgccgcaata tcggttgcgg ttaacaatcg
     2581 atcttc
//
LOCUS       DAOSCH010000176         2415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_28597_length_2415_cov_5.813983, whole genome shotgun sequence.
ACCESSION   DAOSCH010000176 DAOSCH010000000
VERSION     DAOSCH010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28597_length_2415_cov_5.813983"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="PLZ54_08895"
     CDS             <1..399
                     /locus_tag="PLZ54_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ67075.1"
                     /translation="TNAEFTAAEKGSTYKFYSICMDSVGYRENAPENFDQEIFYTRVP
                     ETKISYEWKIYPNPVNDKLFIQLVDAPFVDNYIIELVSMNGSRYAVGKYQTQQIREGI
                     SVPVNNLPAGQYLLRIIFDKNNYSEKVTIQ"
     gene            510..>2415
                     /locus_tag="PLZ54_08900"
     CDS             510..>2415
                     /locus_tag="PLZ54_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67076.1"
                     /translation="MKTYTFPQKRGLIFLCLCIGFFLSSAYGTNPDFSNYIKNLSGEV
                     NTENLNDIVPEIAISGNTIHLVWMEYKYNDVNKIYYRRSTDLGKTWEPPKLLLSIRNF
                     DYVYDGCSIKKLSVEGNNVHICIPDYMYSESGTGRLYYIRSTDNGSTFEAPKEIANTG
                     GWYKKIFTSFIKCVNNHIGIAYTSEVDGIHFIYSTDNGDHFKDTLVVKNSEYHSYVVD
                     FDYDGNQAFILHNYTYYSSGFAVGKMYITTSFDNGETFTTTKISKPYTSGDYTYEKSC
                     TTAQTSSTYFRDYRKKMARWDENIYIVFEELKDGNWHTVLVTSNDNGHTFQSPIVFED
                     TNFTQYSDATTRGSTVEARRNHVYIATQIKNSAYLKISDDGGNTFTPAKNYFATIPVE
                     LYAEDKPYLFYLMIDPADSTGETVHFVGNTHFLATSYDGGHTFSKIINYTNCFNGFSM
                     GTQVLIDQFNVKHWFLVHNPYGGTDTDIYYRQTGNEPAPGNENQYFSVVEPKSDNSKI
                     SLGIGHSPTLNFDSAMTAEMWVKFYPQVTHDAPLLLKIKGCCYSDYNPRGYDIAFRNS
                     NGTIALNSGLQTDKGNFVNWCPSEIKDTLWHHVAFTYDANAGLKNFITYVDGVVKAKQ
                     TVTGKIDAGEG"
BASE COUNT          786 a          428 c          437 g          764 t
ORIGIN      
        1 accaatgcag aatttactgc tgccgaaaaa ggttcaacct acaaatttta cagcatttgc
       61 atggatagcg taggttatcg ggaaaatgca cctgaaaatt ttgaccagga aattttctac
      121 acacgcgttc ctgaaacaaa aatttcgtat gagtggaaaa tttatcctaa tccagttaac
      181 gacaaacttt tcattcaact ggtagatgct ccttttgtag ataattatat cattgaactg
      241 gttagtatga atggcagtcg atatgctgta ggtaaatatc aaacccaaca aatcagagaa
      301 ggtatttctg tgccagtcaa taatttacct gcgggacaat atttgttgag aattattttt
      361 gacaagaaca actactctga aaaagttaca attcaataat ggattcctgt gctaaataga
      421 tgaattaaaa ttctgtttca tctaaccctt tcatttgctg ttctattttt tactgaatag
      481 ttttcttaac ttatttaaaa caagcaatca tgaaaactta cacttttcca caaaaaaggg
      541 gtcttatctt tttgtgcttg tgcataggct tttttctctc ttctgcttat gggacaaatc
      601 cagatttttc caattatatt aaaaatctgt ctggagaagt gaatacggaa aacttgaacg
      661 atattgtacc cgaaattgct atcagtggca ataccattca tctggtatgg atggaatata
      721 agtacaatga tgtcaacaaa atttattatc gtcgatccac agatctgggt aaaacttggg
      781 agccaccaaa attgctttta agtattcgga attttgatta cgtttatgat ggatgttcta
      841 taaaaaaact gtcggtagag ggtaataatg ttcacatttg tattccggat tatatgtatt
      901 ccgagagtgg aaccggtcga ctgtattata ttcgttctac cgataatggc agcacatttg
      961 aagctccaaa agaaattgcc aatacgggag gttggtacaa aaaaattttt acaagtttta
     1021 taaagtgcgt aaacaaccat attggcattg cttacactag tgaagttgat ggaattcact
     1081 ttatctattc caccgataat ggagatcact tcaaagatac attggtagtg aaaaattctg
     1141 aatatcattc ttatgtagtt gattttgatt acgatggtaa ccaggcattt atcctccata
     1201 attatacgta ttattcttcc ggttttgcag ttggtaaaat gtacatcaca acctctttcg
     1261 ataatggtga aacttttaca actaccaaga tttcaaaacc ttataccagt ggcgattata
     1321 cgtatgaaaa atcctgcacc accgctcaaa cttcctcgac atattttaga gattaccgaa
     1381 aaaaaatggc cagatgggac gagaatattt atattgtttt tgaagaattg aaagatggaa
     1441 attggcatac tgttttagta acttccaatg acaacggaca tacttttcag tctcctattg
     1501 tatttgaaga taccaatttt acgcaatatt cagatgctac aactcgcggt tctacagtcg
     1561 aagccagaag aaatcacgta tatattgcca cacaaattaa aaatagtgcg tatttaaaaa
     1621 tttcagacga tggaggaaat acattcacac ctgcaaaaaa ttattttgca acaattcctg
     1681 tagagttgta tgctgaagat aaaccctacc ttttttattt gatgatcgat cccgctgatt
     1741 ccaccggaga aaccgttcat tttgtaggaa atacacattt tcttgcaact tcgtatgatg
     1801 gtggtcatac tttcagtaaa ataatcaatt acaccaattg ttttaacggc ttttcgatgg
     1861 gaacgcaagt actgattgat caatttaatg tcaaacactg gtttttggta cataatcctt
     1921 atggcggaac cgatacggat atttattacc ggcaaaccgg caacgaacct gctcccggta
     1981 atgaaaatca atactttagt gtggtagagc ctaaaagtga taacagtaaa atttcgctgg
     2041 gcattggaca ttctcccaca ttaaatttcg attctgccat gacggccgaa atgtgggtaa
     2101 aattttatcc acaagttact catgatgctc ctcttttatt gaaaattaaa ggttgctgtt
     2161 attctgatta taacccaaga ggttatgata ttgcttttcg gaacagcaat ggaacaatag
     2221 cccttaatag cggacttcaa accgacaagg gaaattttgt caactggtgc ccttccgaaa
     2281 ttaaggatac gctctggcat catgtagctt ttacatacga tgcgaatgcc gggctgaaaa
     2341 attttataac ttatgtggac ggtgtagtaa aagccaaaca aactgttacg ggaaaaatcg
     2401 atgccggcga aggaa
//
LOCUS       DAOSCH010000177         2391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_29011_length_2391_cov_5.535531, whole genome shotgun sequence.
ACCESSION   DAOSCH010000177 DAOSCH010000000
VERSION     DAOSCH010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29011_length_2391_cov_5.535531"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /gene="metG"
                     /locus_tag="PLZ54_08905"
     CDS             <1..1808
                     /gene="metG"
                     /locus_tag="PLZ54_08905"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOJ67077.1"
                     /translation="FLIKKSFEDFGISFDIYSRTTSKIHHELASDIFKTIANKGGFIE
                     KTSEQYYDEEAGQFLADRYIVGKCPHCGNENAYGDQCEACGSSLNPTDLINPRSAITG
                     NIPVLRETKHWYLPLDQHEEWLRHWILEEHKEWKPNVYGQCKSWLDMGLQPRAVSRDL
                     DWGIPVPLEGAEGKVLYVWFDAPIGYISNTKELLPESWEKWWKSEDTRLIHFIGKDNI
                     VFHCIIFPTILKLEGTYILPDNVPANEFLNLEGDKISTSRNWAVWLNEYLEDFPDKQD
                     TLRYVLTANAPETKDNDFTWKDFQARNNNELVAIYGNFVNRTLVLTQNYFDGKVPVRN
                     ELQEIDLQTINELSDIKGNVEKLLDNFKFRDAQKEAMNLARLGNKYLADTEPWKIVKT
                     DKDRVATILNLSLQIAANLSIVFEPFLPFSSEKLRDLLNLETFDWNNLGSIELLKPQL
                     QLKKPVLLFDKIEDSAIEYQVQKLLNTKKKNELSAFKPKDVKSNISYENFEKLDIRVG
                     TVLACQKVPKADKLLQFKIDDGLGGRTIVSGIAQYYPEPEKLIGQQVCFIANLEPRKL
                     KGIVSEGMLLSAEDADGRITLIQPQTTVVNGAEVK"
     gene            2154..>2391
                     /locus_tag="PLZ54_08910"
     CDS             2154..>2391
                     /locus_tag="PLZ54_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOJ67078.1"
                     /translation="MNFSDQLISWYQVHKRDLPWRNISDPYRIWISEIILQQTRVNQG
                     LDYYLRFVERFPDVETLAAAEEDEVLKYWQGLGYY"
BASE COUNT          744 a          405 c          499 g          743 t
ORIGIN      
        1 attttctgat taaaaaatca tttgaagatt ttggaatttc tttcgatatt tattctcgta
       61 ccacttcaaa aattcatcat gaattggctt cggatatttt taaaaccatt gctaataaag
      121 gtggtttcat agaaaaaacg agtgaacagt attacgatga agaagccggt caatttcttg
      181 ccgaccgata tattgttgga aaatgtccgc attgcggaaa cgaaaatgct tacggtgatc
      241 agtgtgaagc ttgtggttcc tcgctcaatc ctactgattt gattaatccc cgctcagcaa
      301 ttaccggaaa tattcctgtg ctgcgtgaaa caaaacactg gtatttgcct ctcgatcagc
      361 atgaagaatg gcttcggcat tggattctgg aagagcacaa agaatggaaa ccaaatgttt
      421 acggacaatg caaaagttgg ctggatatgg gcttacaacc gcgtgccgta agtcgcgatt
      481 tggattgggg tattcccgtt ccactcgaag gggccgaagg aaaagtacta tatgtatggt
      541 ttgacgctcc cattggttat atttccaaca ccaaagaact tttgcctgaa agctgggaaa
      601 aatggtggaa aagtgaagat acccgattaa ttcattttat tggaaaagac aatatcgttt
      661 ttcattgtat tattttccca acaattttaa aactcgaagg aacttatatt ttaccggata
      721 atgtaccggc caacgaattt ttaaaccttg aaggcgacaa aatttcaact tcacgcaatt
      781 gggcagtttg gttgaacgaa tatctggaag attttcccga taaacaggat actttgcgtt
      841 atgtgctgac tgcgaacgct cccgaaacga aagacaacga cttcacatgg aaagactttc
      901 aggctcgaaa caacaatgaa ttggtagcta tttatggtaa ttttgtgaat cgaactcttg
      961 tgttgacaca aaattatttt gatggaaaag ttccggttcg aaatgaattg caagaaattg
     1021 atttacagac aattaatgaa ctctctgata taaagggaaa tgtcgaaaaa ttgctcgata
     1081 atttcaaatt tcgcgatgca caaaaagaag ccatgaatct tgcccggctt ggtaataaat
     1141 atctggctga cacagaaccc tggaaaattg taaaaacaga taaggataga gttgctacta
     1201 ttttgaattt gagtttgcag attgcagcca atctttccat cgtctttgaa ccgtttttgc
     1261 cattcagttc ggaaaaatta agggatttgt tgaatttgga aactttcgac tggaataatt
     1321 tgggcagcat tgagttactg aaacctcagc ttcaattgaa aaagccggtg cttctttttg
     1381 acaaaattga agacagtgct atcgaatatc aggttcagaa actgctaaat acgaagaaaa
     1441 agaacgaact ttcggcgttt aaacctaaag acgtaaaatc caacatttcg tacgaaaact
     1501 ttgaaaaact cgatattcgc gtcggcacag tgttggcttg tcaaaaagtt ccgaaagccg
     1561 acaaactgct acagttcaag attgatgatg gtttgggcgg tcgtactatc gtttccggaa
     1621 tcgctcagta ttatcctgaa cctgaaaagt taattggaca gcaagtttgc tttattgcca
     1681 accttgaacc acgaaaatta aaaggaattg tcagcgaagg aatgcttctc tcggccgaag
     1741 atgctgatgg cagaataact ttgatccaac ctcaaacaac agtagtgaat ggggcagaag
     1801 tgaaatgacc agtttcaagt ttcgagttcc gagtttggag tcctgattat ggattattta
     1861 attgataatt actagtttgt agttttttta gcgatgtttc atgaaatgtc ttcgtcaaaa
     1921 aaaatttgta atcctttggt gttttaatag cggatttaaa attcgcaaat aatcctgtcg
     1981 gattgcaaat ccgacaggac aattcatttt ttgttaatga acgtccattt ttcttatagt
     2041 gaggtttggg gcaaaacctc gtaaatagca ttttatataa agtaaatttc aatcgaggct
     2101 attttttgtt ttttttgcag agttgaaagt ttttctttga attttttgaa aacatgaatt
     2161 tttccgatca acttatcagc tggtatcagg ttcataaacg tgatttgcca tggcgaaaca
     2221 tttctgatcc atatcgtata tggatttccg aaatcatttt gcagcaaacg cgtgtcaatc
     2281 agggattgga ttattatttg aggtttgtgg aacgattccc ggatgttgaa acgttggctg
     2341 ccgctgaaga agacgaggtt ttgaaatact ggcaaggttt gggttattat t
//
LOCUS       DAOSCH010000178         2384 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_29137_length_2384_cov_4.589953, whole genome shotgun sequence.
ACCESSION   DAOSCH010000178 DAOSCH010000000
VERSION     DAOSCH010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29137_length_2384_cov_4.589953"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="PLZ54_08915"
     CDS             <1..274
                     /locus_tag="PLZ54_08915"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOJ67079.1"
                     /translation="DFEGEELNYDMIIFFSPVGVASLLKNFPNFVQGDIQIGCFGKTT
                     AQAVRDAGLRLDLEAPQPGLPSMTMALEHFLKENAKKEANNKKAEK"
     gene            354..746
                     /gene="rnpA"
                     /locus_tag="PLZ54_08920"
     CDS             354..746
                     /gene="rnpA"
                     /locus_tag="PLZ54_08920"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOJ67080.1"
                     /translation="MERTRFLFPKSERLNSKIKIDRLFTEGKAFLVYPLRTVYYVSAE
                     NPAGVEVLISVPKKKFKRAVHRNRIKRLIREAYRLNKNELSDVVLSGQLQMQVAFLYV
                     ADKEINFHELNDKMKQILEQLQKIASEQ"
     gene            743..964
                     /gene="yidD"
                     /locus_tag="PLZ54_08925"
     CDS             743..964
                     /gene="yidD"
                     /locus_tag="PLZ54_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005655675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOJ67081.1"
                     /translation="MKKFFRWLVLLPVYFYRYSISPLFPPSCRYTPTCSQYTIEAIKK
                     YGVFKGGWLSVKRISRCHPWGGSGYDPVP"
     gene            1089..2219
                     /gene="lpxK"
                     /locus_tag="PLZ54_08930"
     CDS             1089..2219
                     /gene="lpxK"
                     /locus_tag="PLZ54_08930"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOJ67082.1"
                     /translation="MVSLLSSFSKNNPKLKKDLKHIVLYPLSLLYGLIVDIRNWLFDE
                     KIFHSVGFDVPTIVLGNLAVGGTGKTPHAEFVLSHLQHEFKTAFLSRGYKRKTKGFVL
                     ADENASAATIGDEPFQIFRKFPNVKVAVDEKRVEGIKRLLKLFPDLQLIVLDDAFQHR
                     YLLPGFSVLLTDYSNLYTSDFFLPAGSLRESKEGSHRANLIVVTKCPENLKPIDKRII
                     EMELHPLPYQQVFFSTFRYHDPVPVFPELTTESWTLSKLREENPAVLMVTGIASPQSM
                     KDFLKDYVSTLKEVSFPDHHKFSKNDFQLIRQEWDALPSENKIIIVTEKDAARLYNDK
                     YLPETLKAKMFALPIEVKFLFDQESLFIQKLKDYVRENSRNR"
     gene            2191..>2384
                     /locus_tag="PLZ54_08935"
     CDS             2191..>2384
                     /locus_tag="PLZ54_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOJ67083.1"
                     /translation="MLEKIQETADFLLAKMPIRPKTGIILGTGLGNLVTQMDIEMEIP
                     YETIPNFPVSTVEGHKGKLIL"
BASE COUNT          698 a          476 c          490 g          720 t
ORIGIN      
        1 cgattttgaa ggcgaagagc tgaattacga catgataata tttttcagtc cggtaggcgt
       61 tgcttccctg cttaagaatt ttcccaattt tgtgcaggga gatattcaga tcggatgttt
      121 tggtaaaact acggctcaag ccgtgcgtga tgccggatta aggctcgatc tcgaagcccc
      181 gcagccgggt ttgccgtcga tgacgatggc tctcgaacac tttttgaaag aaaatgccaa
      241 aaaggaagca aacaacaaaa aagccgaaaa ataaaagaat ttgtcgttaa agtatttatc
      301 ccgaaagtaa atgaattatt tttcgttact tttgggatat tttttttgtg caaatggaga
      361 gaacaagatt tttattcccc aaatcggagc gactgaacag caaaataaaa atagatcgct
      421 tgttcactga aggaaaagct tttctggttt atcctctgag gacggtttat tatgtctctg
      481 ctgaaaatcc tgccggagta gaagtcctga tttccgttcc caaaaaaaag tttaaaaggg
      541 cagttcatcg caaccgtatc aaacggctga taagagaagc ctatcgtttg aacaaaaatg
      601 agctttccga tgttgtttta tccggacaac tacaaatgca agttgcattt ctttatgtgg
      661 ccgacaagga aattaacttc cacgagctga acgataaaat gaaacagatt ttggaacagt
      721 tgcaaaaaat agcttctgag caatgaaaaa attttttcga tggttggttt tgttgccggt
      781 atatttttac cggtacagca tttctccgct ttttccgcca agttgccgtt acacacccac
      841 ctgttcgcaa tataccatcg aggcaataaa aaaatatggc gttttcaaag gcggttggct
      901 ttcagtgaaa cgcataagtc gttgccatcc ttggggaggc agcggctacg atcctgttcc
      961 ctgaaggtac actaaaattt tattatcagc aatattcagc gtttttttac ctttcccgtt
     1021 ttcgtgtttc ttcttctttt ttccttgttg aatgatttgt aagtttcagt ttgtttcaaa
     1081 tcagaaaaat ggtatctttg ctctcttctt tttcaaaaaa caatcccaaa ttgaaaaaag
     1141 acctgaaaca tattgttctt tatccgctgt cccttcttta cggattgatt gtcgatattc
     1201 gcaactggct atttgacgaa aaaatttttc attccgtcgg gttcgatgtg ccaaccattg
     1261 tgttgggaaa tttggctgtt ggcgggacgg gaaaaacgcc acatgcagaa tttgttttgt
     1321 cgcacctgca gcatgaattt aaaactgctt ttctcagcag gggatataaa agaaaaacca
     1381 aaggttttgt tttggcggac gaaaatgcct ctgccgctac cattggcgac gaaccttttc
     1441 aaattttccg aaaatttccc aacgttaagg tggcagtgga cgaaaaaaga gtagagggta
     1501 tcaaacggct gttgaaactt tttcccgatt tgcaattgat cgtgttggac gatgcctttc
     1561 aacatcgcta tttgcttccg ggtttttccg ttttgctcac cgattattcg aacctttaca
     1621 catccgattt ttttctgccg gccggcagtt tgcgcgaaag caaggaaggc agccatcgcg
     1681 ccaacctgat agtggttacc aaatgcccgg aaaacctcaa acccatcgac aaacgcatta
     1741 ttgaaatgga gctgcatcca ttgccttatc agcaagtgtt tttttctact tttcggtatc
     1801 acgatccggt gccggttttt ccggagttga ctactgaaag ttggacattg agcaaattga
     1861 gggaagaaaa tccggccgtt ttgatggtta ccggcattgc ttctcctcaa tcgatgaagg
     1921 attttctgaa agattatgtt tcgactttga aagaagtttc cttccccgac catcacaaat
     1981 tttcaaaaaa tgacttccaa ttgatccgac aggaatggga cgctcttccg tccgaaaata
     2041 aaattattat cgttacggaa aaagatgcgg cacgacttta taacgacaaa tatttacccg
     2101 aaacgctcaa ggcaaaaatg tttgccttgc cgatagaggt taaattttta ttcgatcaag
     2161 aatcattatt cattcaaaaa cttaaagatt atgttagaga aaattcaaga aaccgctgat
     2221 tttttgttgg caaaaatgcc aatacgaccc aaaacaggta ttattttggg aacaggattg
     2281 ggaaatttgg taacccaaat ggatattgaa atggaaatac cttacgaaac cattcccaat
     2341 tttcccgttt caaccgttga gggacacaaa ggcaagctga tact
//
LOCUS       DAOSCH010000179         2374 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_29290_length_2374_cov_3.822768, whole genome shotgun sequence.
ACCESSION   DAOSCH010000179 DAOSCH010000000
VERSION     DAOSCH010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29290_length_2374_cov_3.822768"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="PLZ54_08940"
     CDS             complement(<1..1627)
                     /locus_tag="PLZ54_08940"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOJ67084.1"
                     /translation="MKKLIVTLSIFAVAAIGLFATNYLFVFTSDKSVTAIDRSNIDSI
                     KFTSNQTQLSIYKNNSITTSYALIAIDNLTFGSVNTNEVSIAYSVSGITVTNPFAGKG
                     IDIAINGGDVTVTSKLTDTQINYLLSGTTSDGSLKLYSNYKYQITLNGVNITNTDGPA
                     INIQSGKKCTLVLADGTTNTLADGASYASSTEDQKGTLFSEGQIVFDGTGTLNVSSIS
                     KHAIASDDYISITNGSINVTSAGKDAIHTGDYFKMSNGTLSLSPTGDGIDSEGYVNIS
                     GGSITFTSSVADVKAISSDSIMTVFGGTFNLTVNGNQSKGLKAKQNMTLNGGTYIINT
                     AGSAVLEASGSGYDPSYCTAIKSDSSVIVKGGTFTIKATGAGGKGISADKNIEISGGQ
                     ITITTTGGGAIYTNETGTKDAYNATCLSADQNILIADGTITLSSSGQGGKGISADGTF
                     TMGSTSTTPTLSITTSGTEITSGSTSYASPKALKADGDVKFYSGITTISSANNAIKSE
                     TNIYIEGGNITVTKSYEALEAPYIYIDGGTTDVT"
     gene            complement(1680..2159)
                     /locus_tag="PLZ54_08945"
     CDS             complement(1680..2159)
                     /locus_tag="PLZ54_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ67085.1"
                     /translation="MKKSIRQLFGIIVFLLPYFSTNGFGQTMNIVLPDGTTNSFSLTA
                     DSKIYFENDNLLFNENNFSIPVDQIRKITFSNSSGVNNVSENGKNFFISPNPAKDEIS
                     LKNVPNEICSVKIYNLTGKLMYSSNLNPSSAIINISNFPKGIYLVKVNNSTAKLIKL"
BASE COUNT          653 a          428 c          480 g          813 t
ORIGIN      
        1 cagtaacatc tgttgttcct ccgtcgatgt aaatataagg cgcttccaaa gcttcataag
       61 atttggtcac ggtaatattt cctccttcaa tgtaaatgtt ggtttcagat tttatggcat
      121 tgttggctga agaaatggtc gttatcccgc tgtaaaattt aacatcgcca tcagctttaa
      181 gagctttagg tgaagcataa cttgtgctac cagacgtaat ttctgtccct gaagttgtaa
      241 tggataatgt tggagtagtg gaagtgcttc ccattgtgaa agttccatct gctgaaattc
      301 ctttgccacc ctgtccggaa gaagacaagg taattgtacc atcagcaatc aaaatgtttt
      361 ggtcggctga cagacaagtg gcattatagg catcttttgt ccctgtttca ttggtataaa
      421 tggcgccacc gcctgttgtt gtaatggtaa tttgtccacc ggaaatttct atgtttttgt
      481 cagctgaaat tccttttcct ccggcaccag tagccttgat ggtaaatgtg ccccctttta
      541 ctataactga gctgtcgctc tttatggctg tacaataaga aggatcgtaa ccagagcctg
      601 aagcttccag aactgccgat ccggcagtat tgatgatata tgttccacca tttaacgtca
      661 tattctgctt tgcttttaaa cctttggatt gatttccgtt aactgtcagg ttaaatgttc
      721 ctccgaaaac ggtcatgatg ctgtcggaag aaatggcttt gacatcggca acagagcttg
      781 taaaagtgat acttcctcca gaaatattta cataaccttc cgaatcaata ccatcacctg
      841 ttggtgacag ggaaagcgtc ccattgctca ttttgaaata atctcctgta tgaattgcat
      901 ctttgccggc agaagtaaca ttgatacttc cattagtaat tgaaatgtaa tcatcgctgg
      961 ctattgcatg tttcgagata cttgacacgt ttagcgtgcc ggtgccatca aaaacgattt
     1021 gtccttcaga aaaaagtgtt cctttttggt cttccgtgct gctggcatag cttgcaccat
     1081 ctgccagtgt gttagtggtt ccatctgcaa gaacaagcgt gcattttttt cctgattgga
     1141 tattaatggc tggtccgtca gtatttgtaa tattaacgcc gttgagggtg atttggtatt
     1201 tataattgct gtaaagtttt aatgagccat cagacgttgt ccctgaaaga agatagttta
     1261 tttgcgtgtc agtcagcttt gatgttacgg ttacatcgcc tccgtttatt gctatgtcga
     1321 tgcccttgcc tgcaaaagga ttggttactg taattcctga gacagaatag gcaattgaaa
     1381 cttcattggt atttacactt ccaaaggtaa gattatcgat ggcaataaga gcataacttg
     1441 ttgttataga attgtttttg taaatagaaa gttgtgtttg attggaagta aactttatac
     1501 tgtcgatatt cgagcgatcg atggcagtaa ccgatttatc gctggtaaaa acaaaaagat
     1561 aatttgtggc gaataaacct atcgctgcaa ctgcaaatat tgagagagta acaattaatt
     1621 ttttcatata ctcaaatttt taattattct gttttctgat taaagtcatg tttttgacat
     1681 tacagtttaa tcaattttgc agttgaatta ttaactttta ccagataaat tcctttcggg
     1741 aaattactta tattaatgat tgcagaagaa gggttcaaat tgctcgaata catcaatttc
     1801 ccggttaaat tgtaaatttt taccgagcaa atttcattcg gaacattttt caagctaatt
     1861 tcgtcttttg ccggattggg gctgatgaaa aaattctttc cattctccga aacattattg
     1921 acgccggatg aattggaaaa tgttattttt cggatttgat cgacaggtat cgaaaagttg
     1981 ttttcgttga aaagcagatt gtcgttttca aaatagattt ttgagtcagc agtaagagaa
     2041 aacgaattgg ttgtcccatc aggaagcaca atgttcatcg tctgcccaaa tccgtttgtc
     2101 gagaaatatg gcaacaggaa tactattatt ccgaataatt gtcgaataga ttttttcata
     2161 atgttgaggg tttaaaattt tcctgatatt cccattttgt ttttaaattt gttggttgca
     2221 atttcagaga aatttcggga gtttatttta cagcactggg aattttcaga taacatcagc
     2281 atcgggaccg tgtcttccgg gtcctccatc aaagcctcga cgtggcatca tatcgttttg
     2341 atttgcaccg tttttgaaaa tattgaagcg gtat
//
LOCUS       DAOSCH010000180         2361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_29508_length_2361_cov_6.142671, whole genome shotgun sequence.
ACCESSION   DAOSCH010000180 DAOSCH010000000
VERSION     DAOSCH010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29508_length_2361_cov_6.142671"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="PLZ54_08950"
     CDS             complement(<1..525)
                     /locus_tag="PLZ54_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOJ67086.1"
                     /translation="MIQIKHVEFSYSRNAQLFTGLELSLSPGHIHGILGKNGEGKSTL
                     LKMISGMIFPQKGEIEVLGHEPRKRQPEMLREIFFLPEELPPITLSIENYEKVYAPFY
                     PHFNHEQFQGYLQEFEINSGKSQINKLSYGQKKKVFMAFGLATNTKIVLLDEPTNGLD
                     IPSKAQFRKMTASAL"
     gene            complement(783..923)
                     /locus_tag="PLZ54_08955"
     CDS             complement(783..923)
                     /locus_tag="PLZ54_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67087.1"
                     /translation="MKLFEQLEPHRNDYFGFFSVDTLELVFNDWYQRKKKVKTLHRFK
                     LE"
     gene            complement(1727..>2361)
                     /locus_tag="PLZ54_08960"
     CDS             complement(1727..>2361)
                     /locus_tag="PLZ54_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOJ67088.1"
                     /translation="GAEQSGFIADLGYETYQKVLNEAVQELKDEEFADLYAEVEEKTS
                     TASAYVTDCQIDSDLELLFPSDYVENVSERINLYRQLDSIENENQLTAFEKEIEDRFG
                     KIPPQGKSLILMVRLRWLAINLGIEKMVLKNQQMTAYLVSNQKSHYYQSDTFGEILRY
                     AASHPHQCQLREKNGRRSIIFLKVTSVEKTWEILLEINRNLETARRLKAV"
BASE COUNT          700 a          405 c          459 g          797 t
ORIGIN      
        1 caaagcagaa gccgtcattt ttctgaattg ggctttggaa ggaatatcca aaccattggt
       61 cggctcgtcc agcaatacaa ttttggtgtt ggtggccaat ccgaaagcca taaaaacttt
      121 ctttttctgt ccgtacgaaa gtttgttgat ttgcgatttt cccgaattta tctcaaattc
      181 ctgcaaatat ccttgaaatt gttcgtgatt gaaatgagga taaaagggag cataaacttt
      241 ttcgtagttt tcgatgctca aagttatggg cggcaattct tcgggcagga aaaaaatctc
      301 acgcaacatt tcgggctgac gttttcgggg ttcgtgcccg agaacttcaa tttctccctt
      361 ttgtggaaaa atcatgcctg aaatcatttt caacaaagta cttttgcctt ccccgttttt
      421 tcccagtatg ccgtgaatgt ggccgggaga aagcgacagt tccaaaccgg taaacaattg
      481 agcgttgcgg ctgtagctga attcgacatg tttgatctga atcataataa attgtttatt
      541 gaattagtgt aatagttaaa tagtacacca caaaagtaat tgctttttta taaagttgtt
      601 caaatacata agttaaattt tgttaattat caaaattaat ttgcaacaat aagattatca
      661 gcgtgtttaa ttttggaaaa ctgagaagaa aaaccaaaaa gttttgaaat atttcgtctc
      721 attttttcca agtgcatcaa cttgcgggca gagagatagg tttgttggcg aggaaatttg
      781 ttctattcca gtttaaaacg gtgtaatgtt tttaccttct tttttctttg gtaccagtcg
      841 ttgaaaacca attcgagcgt atcaacggaa aagaaaccaa aataatcgtt tctgtgtggt
      901 tcgagttgtt cgaaaagttt cattttattt tatttgctaa aatccgataa atgattcttt
      961 caattttttc tgctctaaaa ttccaaaatt ttaaaatata tgattttagt acatgacaaa
     1021 aattgagaca tttgttttat atatctgtaa aacaggacat ataagtcaag ctcttggggt
     1081 tgaccttgtc cggtgagccg aaaaacaagt gagaaaagcg tttaaaaatc gtccttgttt
     1141 gagttccgac gtatttcgtc ggaatgagtt tggacgattt cagcttttcg agcgtaagtt
     1201 tttcgagtga agtggacaca gtcttgaatt tttttgctta cttttttgtt tcaagacaaa
     1261 aaagtaagta gggtttgggg caaagcccct ttattaaata tctgaaaata aaaaatataa
     1321 atgtttttaa aatttttttg tcatgtacta agatatatga tagccggaag aaatatacat
     1381 tttgggagaa gaaaaaatta tcggtttgat tatcagtttg ttttgttgac aaaggcgaaa
     1441 aactgcattt ttaacaaaat tttctgtgaa aaagggcatc aattcgagaa taatgtttta
     1501 tatttgcatt aaagaaaaac gagccgcatt gtttagagac tggaaaactc atcagtaaag
     1561 aattcaaacc gagaaaagtc tgactttcaa tggcgggctg cgccttcggg tattcgtcag
     1621 caatgacgga cggcagatta caaagaaagt caacgtctca aatcctattt aataggagtt
     1681 ttctcacaac aaacagtgcg gttttcgttt tttcttactt ctttatttac accgccttta
     1741 gccgtcttgc agtttccaaa ttacgattga tttccaaaag aatttcccac gttttttcta
     1801 ccgatgttac ttttaggaag ataatagacc gtcgaccgtt tttttcgcgg agttgacatt
     1861 gatgtggatg cgaggcggca tatctcagta tttcgccaaa agtatcggat tggtaataat
     1921 gagatttttg atttgaaacg agataagctg tcatttgttg gtttttcaaa accatttttt
     1981 cgatacccaa atttatggcc agccatcgca gccgtaccat caaaatgaga ctttttccct
     2041 gaggtggaat tttaccgaag cggtcttcga tttccttttc aaaagctgtc aactggtttt
     2101 cgttttcaat gctgtcgagt tgtcggtaca gattgattcg ttcagaaaca ttttcgacat
     2161 aatcggaagg gaacaataat tccaaatcag aatctatctg acaatcggtt acataagcgc
     2221 tggcagttga ggttttttct tccacttcgg catacaagtc ggcaaattct tcatctttca
     2281 gttcctgaac agcttcgtta agcactttct gataagtttc gtaaccgagg tcggctataa
     2341 atccgctttg ctctgctccc a
//
LOCUS       DAOSCH010000181         2354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_29634_length_2354_cov_7.189213, whole genome shotgun sequence.
ACCESSION   DAOSCH010000181 DAOSCH010000000
VERSION     DAOSCH010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29634_length_2354_cov_7.189213"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="PLZ54_08965"
     CDS             <1..1412
                     /locus_tag="PLZ54_08965"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ67089.1"
                     /translation="FERTVHYHIGDIENYFYVYQLSQISQIQVDEIWKDKLQSLFLTL
                     AKIAYPDRSAPVLQDCTDRTWAESEDISETMTLGYLLFKNPEMGYFAQNRVDAGMFWF
                     LQDTQLNLLNAIEKKKPSFGSLSFPDTRYYVMRQGWSKNDKMMIISAGLDPNKPDHQH
                     GDILGVQAMANSKIILPNYQVFYKLPDLELFKNSLTKNVALVDNELQGKEWIPNKGKT
                     GFGKFGILPKPQTLLWQSNKDYDVFVGSHDGFKNIEVDYSRQVIFVKNDFWIVKDNFT
                     SEKPHTYKQVWQGHYTVEQKPELLRSVFPDAMGCDIFQLFPVSHVSQSGARGKQWSIV
                     EKENEKDFNFVTIVYPFNGYGNRINELDAQPDLKGWKVNQLSYVAEGTRFCSLTNQQN
                     AYLLNVKKIKLKNLIITFSKETDLFVQEKDNAVTISALGCQQVLVNISGCNSISINDK
                     TIVNSATLHPGDKLICKLK"
     gene            1445..2338
                     /locus_tag="PLZ54_08970"
     CDS             1445..2338
                     /locus_tag="PLZ54_08970"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ67090.1"
                     /translation="MKKILLSALLLSTFVIGILAQENSSESVKKVQFPENYQSKLDVV
                     YTRVNGWDGRMDIYFNPASEQPTPAVIHIHGGGWNHGTKESQTGFGSWFKAGFAVINV
                     EYRLVQIAPAPAAIEDVRCALTYVKTHAKELNIDPNKIVVEGNSAGAHLALMAGLLEN
                     DRRFDTHCPSNIDMRVAAIVSNYAATDFSGNNIDAKKWKSLANWLGDKVDDKSFRDAV
                     SPLTYVKKTSPPVFIVHGNADPIIPYQQSAILHQRLDECGVYNIFITVDGGGHGGFDK
                     GEKAEINRALMEFLSKTVFQK"
BASE COUNT          758 a          408 c          480 g          708 t
ORIGIN      
        1 agtttgaacg cactgttcat taccatatag gtgacataga aaattatttt tatgtctatc
       61 aactgtcgca aatcagccaa attcaagttg atgaaatatg gaaagataaa ttgcaatcac
      121 tttttttgac gcttgccaaa attgcatatc ccgatagatc cgctccggtt ttgcaggatt
      181 gcactgatag aacatgggca gaatccgaag atatttctga aaccatgaca cttgggtatt
      241 tactttttaa aaatcctgaa atgggctatt ttgctcaaaa tcgggtagat gcaggaatgt
      301 tttggttcct acaggataca caattaaatc tgttgaatgc cattgaaaag aaaaaacctt
      361 cgtttggttc cctttcgttt cccgacacac gctattatgt gatgcggcaa ggctggagca
      421 aaaacgacaa aatgatgatt attagtgccg gattggatcc aaacaaacca gaccatcaac
      481 atggtgatat actcggcgta caagccatgg caaatagtaa aatcatttta cctaattatc
      541 aggtttttta taaattaccc gatttggaat tatttaaaaa ttcattaacg aaaaatgttg
      601 ccttggtaga caatgaactt cagggtaaag aatggattcc caacaaaggt aaaaccggtt
      661 ttggcaagtt tggaatattg cccaaacctc agacccttct ttggcaaagc aataaagact
      721 atgatgtttt tgttggcagc catgatggtt ttaaaaatat cgaagtcgat tattcgcgtc
      781 aggttatttt cgtaaaaaac gatttttgga ttgtaaaaga caattttact tcagaaaagc
      841 cacatactta caaacaggtt tggcaaggac attatacggt agaacaaaag ccggaattgt
      901 tgcgttccgt ttttccggat gcaatgggtt gtgatatttt ccaattgttt cctgtaagtc
      961 atgtttctca atcgggggca agaggaaaac aatggagcat tgttgaaaaa gagaatgaaa
     1021 aagatttcaa ttttgttaca atcgtttatc ctttcaatgg ttatggcaat cgaatcaacg
     1081 aactggatgc tcaaccggat ttaaagggct ggaaagtcaa tcaactttcg tatgtggccg
     1141 aaggaacccg attctgttca ttaaccaatc agcagaatgc ttatttactg aatgtcaaaa
     1201 aaataaagct taaaaacttg ataattacct tctcaaaaga aaccgatctt tttgttcagg
     1261 aaaaagacaa tgcagtaacg atttcggctt tgggttgtca gcaagtgctg gtgaatattt
     1321 cgggatgtaa ctccatcagt atcaatgata aaaccattgt aaattcagct accttgcatc
     1381 ccggcgataa gctgatttgt aaattgaaat aagcaattaa atgcgattca aatactattt
     1441 aaaaatgaaa aaaattcttc tatccgcttt attattatcc acgtttgtca ttggaatttt
     1501 agcacaagaa aattctagtg aatcagtcaa aaaagttcag tttccggaaa attatcaatc
     1561 gaaattggat gtagtttata cccgtgtaaa cggttgggac ggtcgtatgg acatttattt
     1621 caatccggca agcgaacagc ccacacctgc ggttattcat attcacggcg gtggttggaa
     1681 tcacggaaca aaggaaagtc agactggttt tggttcatgg tttaaagcgg gatttgccgt
     1741 aataaacgta gaatacagat tggttcagat agctccagct cccgctgcta ttgaagatgt
     1801 tcgttgtgct ttgacttatg tgaaaacgca tgccaaagaa ttgaatatcg atcccaataa
     1861 aattgttgtg gaagggaatt cggctggtgc ccatttggca cttatggcag gtttgctcga
     1921 aaatgatcgc agattcgata cccattgtcc cagtaatatc gacatgagag tagctgcgat
     1981 tgttagcaat tatgcagcaa cagatttttc gggcaacaac attgatgcta aaaaatggaa
     2041 atcgctggca aactggctgg gagataaagt agatgacaaa tcttttcgtg atgcagtttc
     2101 ccctttaacc tatgtaaaaa aaacaagtcc tcctgttttt attgttcatg ggaatgctga
     2161 tcctatcata ccttaccagc aatctgctat tttgcaccaa cgccttgatg aatgtggagt
     2221 ttacaacata tttattacag ttgatggtgg agggcatggt ggattcgaca agggggaaaa
     2281 ggctgaaatt aatagggcat tgatggaatt tctttcaaaa accgtttttc aaaaatagtc
     2341 aaaaaatatt cttt
//
LOCUS       DAOSCH010000182         2337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_29942_length_2337_cov_8.214724, whole genome shotgun sequence.
ACCESSION   DAOSCH010000182 DAOSCH010000000
VERSION     DAOSCH010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29942_length_2337_cov_8.214724"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..1208)
                     /locus_tag="PLZ54_08975"
     CDS             complement(24..1208)
                     /locus_tag="PLZ54_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HOJ67091.1"
                     /translation="MRGFKTIKKQPTQPQSQPQYEVADVLNKLGSKLEDLGLNSWQLR
                     TLFALKKCRTSALGGHIDACDECGNISISYNSCRNRHCPKCQGRNREDWIQTRETELL
                     PVPYFHVVFTLPEVLNKTALHEPKMLYDILFESAWETLQTFGKNKNLQMGMIAVLHTW
                     GQNLSLHPHLHCIVPGGGVDENGAWKNIKNDGKFLFSVKALSKVFRAKFCEKLKANLK
                     DKFNENQENEYEKIRQSLWEKPWVVYAKKPFGSPKSVVEYLGRYTHKIAISNGRIRGI
                     DDKTVTFDYKDYRQKGIKKQMVLSHEEFIRRFAMHILPKRFVKIRHYGFLSSTWKRIK
                     LKKLQQKLGIQPKEKPLPKPFQPKCSCCKTGNLVTIAMFDLRGPPQWFLEMSQSQPTP
                     KK"
     gene            complement(1205..2002)
                     /locus_tag="PLZ54_08980"
     CDS             complement(1205..2002)
                     /locus_tag="PLZ54_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOJ67092.1"
                     /translation="MSLHFGKIPTELDSEQIHDYLFYLQKKSKSPSQSYFKHTVYGLR
                     FLLKSEGLSYDYLSLPEIKREKKLPVVLSKQEVWQMLSGCKLLKHKILIGILYGCGLR
                     CMEVRNLRLCDLDFDRKQLKVVQGKGKKDRYLPLSEHLIRGLKKYIEAEKPENYLFGM
                     PREGRAGASTSLSTGFDSRYSQRGVQWVVKQASKTAKILKEVSVHTLRHSFATHLLED
                     GMDILSIKNLLGHESIDTTLIYLQIAQLSTQKLFSPLDTLFSEFGKK"
BASE COUNT          639 a          492 c          422 g          784 t
ORIGIN      
        1 cataatttcc cttacccaaa aatctatttt ttaggcgttg gctgactttg gctcatctcc
       61 aaaaaccatt gtggcggtcc cctcaaatca aacattgcaa tggtgactaa attccctgtt
      121 ttacaacaac tgcatttcgg ttgaaacggc tttggcaaag gtttttcttt gggctggatg
      181 cctaattttt gttgcagttt tttaagctta attcgtttcc aagtgctgct caaaaaacca
      241 taatgacgga tttttacaaa tcttttcggc aaaatatgca tcgcaaaacg gcggataaac
      301 tcttcatggc ttaaaaccat ctgctttttg atgccttttt gacggtaatc tttgtaatcg
      361 aacgttactg ttttatcatc aattccccta attctaccat tgctgatggc aattttgtgg
      421 gtgtatctgc ccaaatattc caccacagat tttgggcttc caaatggctt tttggcgtaa
      481 actacccaag gtttttccca cagactttgc ctgatttttt cgtattcatt ttcttgattt
      541 tcattgaatt tatcctttaa attagctttc agtttctcac aaaatttagc cctgaaaact
      601 ttactcaaag cctttaccga aaacaaaaat ttaccgtcat ttttgatgtt tttccaagct
      661 ccgttttcat ccacgccacc accgggaaca atgcagtgca aatgcggatg aagactcaaa
      721 ttctgtcccc aagtgtgcaa aacagcaatc attcccattt ggagattttt gtttttgcca
      781 aacgtttgaa gcgtttccca agccgattca aataaaatat catacaacat cttgggctcg
      841 tgaagcgctg ttttgttcaa tacttcagga agcgtaaaaa ctacgtgaaa ataaggcaca
      901 ggaagcagtt cggtttcccg tgtttgtatc caatcttctc ggttccgtcc ttggcatttc
      961 gggcaatgac ggtttcggca ggagttgtag ctgatgctga tatttccgca ttcgtcgcaa
     1021 gcatcgatat gtcctcccaa agccgatgtt ctgcactttt ttaatgcaaa taaagttctt
     1081 aattgccagg aatttaatcc taaatcttcc aattttgaac ctaatttgtt taaaacatcg
     1141 gcgacttcgt attgaggttg agattgaggt tgagttggct gtttttttat ggttttaaaa
     1201 cctctcattt cttcccaaat tctgaaaaaa gggtatcgag cggtgaaaag agtttttgtg
     1261 tggaaagttg tgcaatttga agataaatca acgtcgtgtc aatactttcg tgaccgagaa
     1321 gatttttgat gcttaaaata tccatcccat cttccagaag atgcgtcgca aaactgtgcc
     1381 gaagcgtatg aacactcact tctttcaata tttttgccgt ttttgatgcc tgttttacca
     1441 cccattgaac gccccgttgc gaatatctgg aatcgaagcc tgtgctgagc gaagtcgaag
     1501 cgccagctct tccttctcgt ggcattccaa agagataatt ttctggtttt tccgcttcga
     1561 tatacttttt taatccccga atcaaatgct ccgaaagtgg caaatagcgg tcttttttgc
     1621 cttttccctg aaccactttc aactgttttc gatcaaaatc taaatcacat aaacgcagat
     1681 ttctaacctc catacagcgc aatccgcagc cgtaaagaat cccgattaaa attttatgtt
     1741 tcagaagttt acatcctgaa agcatttgcc aaacctcttg tttactgaga actacaggca
     1801 gttttttctc tcttttaatt tccggaagac tcagataatc gtagctcaaa ccttccgatt
     1861 tcagtaaaaa tcgaagtccg taaacggtat gtttaaaata cgactgtgaa ggtgattttg
     1921 attttttctg aaggtaaaaa aggtaatcat gaatttgctc tgaatccaat tctgtaggaa
     1981 ttttcccgaa atgaagcgac accgacgcga cgtgtctaga gtaattgttg aacgtacttt
     2041 gactacgccc caaaaccgaa acggtacgtt caaatcgacc taaaagttct gtaaaacctg
     2101 gaacttcgcg ctttgcctga ttgatgagtt tgttttctct aagctcttct aaactctttt
     2161 ttttgttgtc attgtattga attttaatta tctttacaaa gtaactaatt tataccaacg
     2221 gtaaagctac cgaaggttta gttcaacaag ggttttgcaa tagtggggcg aaactgcaaa
     2281 gttcaacggt agttcttcgg ttcaactatt ctacaaaatt gaagatttgt gcttcga
//
LOCUS       DAOSCH010000183         2316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_30334_length_2316_cov_7.226006, whole genome shotgun sequence.
ACCESSION   DAOSCH010000183 DAOSCH010000000
VERSION     DAOSCH010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30334_length_2316_cov_7.226006"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..910)
                     /locus_tag="PLZ54_08985"
     CDS             complement(17..910)
                     /locus_tag="PLZ54_08985"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOJ67093.1"
                     /translation="MKKVLLSTLLFSTIIFGIFAQENPSEQIKKVKFPENYQSKLDVV
                     YTRVNNWEGRMDIYYNPTSVSPTPAVIHIHGGGWNHGTKESQTGFGSWFKAGFAVINV
                     EYRLVQVAPAPAAIEDVRCALTYVKTHAKELNINPDKIVIDGNSAGAHLALMAGLLEN
                     DRKFDTNCPSNIDMRVAAIVSNYAPTDFSGNNIDAKKWKSLANWLGDKVDNQSFRTSV
                     SPLTYVNKTSSPVFIVHGNADPIVPYQQSVALHEKLDQYGVYNVFITVDGGGHGGFDK
                     EKKTEINRALMEFLSKTVFQK"
     gene            complement(943..>2316)
                     /locus_tag="PLZ54_08990"
     CDS             complement(943..>2316)
                     /locus_tag="PLZ54_08990"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ67094.1"
                     /translation="ENYFYVYQLAQISQIQVSQVWKNKLQSLFTTLAKIAYPDKSAPV
                     LQDCTDRTWAESEDISETMTLGYLLFNDPELGYFAQNRVEAGMFWFLQDKQLNLLNAI
                     EKKKPSFSSLSFPDTRYYVMRQGWNKNDKMMIISAGLDPNKPDHQHGDMLGVQAMANG
                     KIILPNYQVYYELPDFELFKNSLVKNVALVDNELQGKEWTPNKGKTGFGKFGILPKPQ
                     TILWQSNKDYDIFVGSHDGFKNVGVDYSRQVIFVKNDFWIIKDNFSSDKLHTYKQVWQ
                     GHYTIEKKPELLRSVFPDAMGCDILQLLPVSQVSQSGARGKQWSIVEKNDEKDFNFVT
                     VIFPFNGYGNRINEFEVQPDLKGWKVNQLSFAAEGNRFCSISNSQNSYLLNVKKIALK
                     NLILTFSKETDIFIQEKENAVLISALGCQQVLMNISGSISNSMNEKTIGNSTPFNPGE
                     KITCKIK"
BASE COUNT          697 a          460 c          402 g          757 t
ORIGIN      
        1 aaagaatatt ttttgactat ttttgaaaaa cggtttttga aagaaattcc atcaacgcac
       61 ggttaatttc agttttcttt tccttatcaa agccaccatg cccaccacca tctacggtaa
      121 taaacacgtt gtaaacgcca tactgatcaa gtttctcatg taaagcaacc gattgctgat
      181 aaggaacaat aggatctgca tttccatgca caatgaatac gggagagctt gttttattca
      241 catatgtcaa aggagaaact gaagtgcgaa aagattggtt gtcaacttta tctcccaacc
      301 agttagcgag tgatttccat tttttagcat caatattgtt tcccgaaaaa tcggttggtg
      361 cataattact gacaatggca gccactctca tatcaatgtt actgggacaa ttagtatcaa
      421 acttgcgatc gttttccagc aaaccggcca taagtgccaa atgagctccg gcagaatttc
      481 cgtcaattac aattttatcg ggattgatat tcaattcttt ggcatgagtt ttcacgtaag
      541 tcaaggcaca acgaacatct tcgatagcag caggagccgg agctacctga accagtctgt
      601 attctacatt tattactgca aatccggctt taaaccaaga gccaaagcct gtttgacttt
      661 cttttgtgcc atgattccat ccgccgccat ggatatgaat aaccgcaggt gtgggcgaaa
      721 cactggttgg attgtaataa atgtccatac gaccttccca attgtttact cgggtatata
      781 caacatccaa tttcgattga taattttccg gaaacttcac ttttttaatt tgctcgcttg
      841 gattttcttg agcgaaaatt ccaaaaataa ttgtcgagaa caacaaggtg gataaaagta
      901 cctttttcat ttttaaattg tttttaaatg gcatttaatt ctttatttta ttttacaagt
      961 gattttttca cccggattga aaggagttga atttccaatt gttttttcat tcatactatt
     1021 ggaaatactc ccagaaatat tcatcagcac ttgctgacaa cctaatgcag aaatcaacac
     1081 cgcattttct ttctcctgta taaaaatatc agtctctttt gaaaaggtaa gtattaaatt
     1141 ttttagtgct atttttttga cattcagcaa ataagaattt tgcgaattac tgatagaaca
     1201 gaatcgattc ccttcggctg caaatgaaag ctgattgact ttccagcctt tcaaatccgg
     1261 ttgaacttca aattcgttga ttcgattgcc gtagccgttg aaaggaaaaa tgaccgttac
     1321 aaaattgaag tctttttcat cgtttttttc aacaatgctc cactgtttac ctcttgcacc
     1381 cgattgagaa acttggctga caggcagcaa ttgcaaaata tcacacccca ttgcatccgg
     1441 gaaaacagaa cggagcaatt caggcttttt ctcaatcgta taatgtccct gccaaacttg
     1501 tttgtaagtg tgcaatttat cagaggaaaa attgtctttt ataatccaga aatcgttttt
     1561 tacaaaaata acctgacgcg aataatcgac tcctacattt ttaaaaccat catggctgcc
     1621 aacaaaaatg tcatagtctt tattgctttg ccaaagaatg gtttgaggtt tgggtaaaat
     1681 gccaaacttt ccaaaacctg tttttccttt gttcggagtc cattctttgc cttgaagctc
     1741 attgtctacc aaagccacat tttttactaa agaattttta aataattcaa aatcaggcaa
     1801 ttcgtaatag acttgataat ttggtaaaat aatttttccg tttgccatcg cctgcacgcc
     1861 aagcatgtcg ccatgttgat ggtcgggttt gttcgggtcc aaacctgcac tgataatcat
     1921 cattttgtcg tttttgttcc agccttgcct cataacataa tagcgtgtat cgggaaacga
     1981 aagggagcta aaagaaggtt ttttcttttc aatagcattc aaaagattta actgtttgtc
     2041 ttgaaggaac caaaacattc cagcttcaac ccgattttgt gcaaaatagc ccaattcagg
     2101 atcgttaaaa agcaaatatc cgagcgtcat agtttcagaa atatcttcag attccgccca
     2161 agttctgtcg gtgcaatcct gcaatacagg agccgatttg tcgggataag caattttggc
     2221 aagtgtcgta aaaagtgatt gtaatttgtt tttccacact tgactcactt gaatttgact
     2281 gatttgtgcg agttgatata cataaaaata attttc
//
LOCUS       DAOSCH010000184         2227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_32032_length_2227_cov_4.176335, whole genome shotgun sequence.
ACCESSION   DAOSCH010000184 DAOSCH010000000
VERSION     DAOSCH010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32032_length_2227_cov_4.176335"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLZ54_08995"
     CDS             <1..261
                     /locus_tag="PLZ54_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOJ67095.1"
                     /translation="SVQDGRLDVSIMSNFPIIALPTLALQLFTKTISNDLFINTFRMS
                     EITVFRPAPGPFHYDGEPYEEGKEINIRVVPGGLKVLVRKRF"
     gene            358..2046
                     /gene="gldG"
                     /locus_tag="PLZ54_09000"
     CDS             358..2046
                     /gene="gldG"
                     /locus_tag="PLZ54_09000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOJ67096.1"
                     /translation="MRNIIQHKQLFFWGVFLLLSLVLSFVHYRVDLTSEKRYSLSEPS
                     KKLMKKLDFPLKVKIYLDGSLNSGFLRLKKSTVEMLEELKVYSSKPIAIEFEDPSSVV
                     KPEEREKHYAELESRGLTPTNVYERDNDGKIIRKTIFPWLEMEYKGRKVLVNLLKNIP
                     GNSGDENLNISIIELEYEITDAVRRLVNTSVDKIAFLEGHDELSEAATYDISKSLSRY
                     FQIDRGRIGNDASILHPYKVVIVAKPQLPFSEKEKLIIDQYIMNGGKVLWLIDGVRLA
                     EENFSVTGTSPAIPLDLNLSDLLFRYGIRIKPVILQDVQCAQMPVNVAKPGKAPHFEP
                     VPWFFAPLLLTSYAHPVTRNLATVRSEFCSAIDLVGENQQVSAVLLLATSDNTHVIGT
                     PTTVDLRDMPSANDKNYFNTGYVPVGVLLEGNFESVFTNRMLPDSVVNISSIRPVSVN
                     TKQIFVADGDIIKNEISIVQGDSIYLPLGFDRLTGLQYGNRDFIENAVLYLADDEGWM
                     QLKNKTLKLRLLNRQVVYDQKLKWQLVNVLLPLVILAIFGFVFQLIRKRKFTRS"
BASE COUNT          647 a          420 c          447 g          713 t
ORIGIN      
        1 agcgttcagg atggacggct cgatgtctcc attatgagca attttcccat tattgctctt
       61 cctacgttag cactgcaact ttttaccaaa actattagca acgatttgtt tatcaatact
      121 ttccgaatga gtgaaattac ggtttttcgt cccgctccgg gtccttttca ttacgatggt
      181 gaaccttatg aagaagggaa ggaaatcaat ataagagttg ttccgggcgg tttaaaagta
      241 ttggttagaa aaagattctg aatgtcaaat ttttttcgga tgcatttttg ctcaatggaa
      301 atgattaatt ttgcattcga atttcttctc gatttttact gaagaatctt ctgtaacatg
      361 cgtaatataa ttcaacacaa gcaattattt ttttggggag tttttttgct gttgtcgctg
      421 gtgctttcct tcgttcatta cagagtagat ctgacgtccg aaaagcgcta cagtttgtcg
      481 gaaccatcca aaaaactgat gaaaaagctc gactttccgc tgaaagtaaa aatttatctc
      541 gatggcagtc tcaattccgg ctttttaagg ttgaaaaaat ctacggtcga aatgttggaa
      601 gaactgaaag tttacagttc caagcccata gcaattgaat ttgaagatcc ttcatctgtt
      661 gtcaagcctg aagaaagaga aaagcattat gccgaattgg aatcgcgcgg attgacacca
      721 accaatgtat atgaacgcga taacgacggc aaaataatca ggaaaacaat ttttccgtgg
      781 ttggaaatgg aatacaaggg acgcaaggtt ttggtaaatc tgttgaaaaa cattcccggc
      841 aattcgggcg acgagaatct gaatatttcc attatcgaac tggagtatga aattacggat
      901 gctgtccgtc gtttggttaa cacgtcagtt gataaaattg cttttctgga aggacatgac
      961 gaactgtccg aagctgcgac ttatgacatt agcaaatcgt tgagccgtta tttccaaatc
     1021 gatcgaggtc gcataggaaa tgatgcgtcc attctccatc cctataaagt ggtaattgtt
     1081 gccaaacctc aacttccttt cagtgaaaaa gaaaagctga ttatcgatca atacattatg
     1141 aatggaggaa aagtgctttg gttgattgat ggggtgcgcc tggctgaaga aaatttttct
     1201 gttaccggca catcacctgc cattccgctc gatttgaatc tttccgacct gctttttcga
     1261 tatggaattc gtatcaaacc ggttattttg caagatgttc aatgtgcaca aatgcctgtt
     1321 aatgtggcaa aacccggaaa agctcctcat ttcgaacctg ttccctggtt ttttgctcca
     1381 cttttgctca cttcttatgc tcatcccgtt acccgtaacc ttgcgacggt tcgctccgaa
     1441 ttttgttctg ccatcgattt ggttggtgag aatcaacaag tttcagccgt tttgctactt
     1501 gcaacttccg acaatactca tgtgattgga actccaacca ctgttgatct tcgggatatg
     1561 ccatctgcaa acgacaagaa ttatttcaat actggatatg ttccagttgg tgttcttttg
     1621 gaaggcaatt ttgaatcggt ttttaccaac cgaatgttgc ccgatagcgt ggtgaacatt
     1681 tcgagtattc gtcctgtaag tgtaaacaca aaacaaattt ttgtggcaga tggagatatt
     1741 atcaaaaatg aaatttccat agttcagggt gacagtatct acttgccttt gggtttcgac
     1801 agattgacag gtttgcaata cgggaatagg gattttattg aaaatgcagt cctctacctt
     1861 gccgatgatg aaggatggat gcaactgaaa aacaaaacat taaaacttcg tttgctcaac
     1921 agacaggttg tttacgacca aaaactaaaa tggcaacttg tcaacgtgtt gctaccgctt
     1981 gttattcttg cgattttcgg ctttgtattt caacttatca gaaaaagaaa gtttacgcgc
     2041 tcctgagaaa attattccat aaattttatt tcaatttttt tgactgatat tcagctattt
     2101 gtcgattgtg aaaaatcaat tctttccaaa aaattgttag tgtaaaatta attttgggaa
     2161 atgaaaataa ttgctaacgt tccaataatc ctttgtaata ggactgcaaa aacactttcc
     2221 cgttaat
//
LOCUS       DAOSCH010000185         2219 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_32216_length_2219_cov_6.748614, whole genome shotgun sequence.
ACCESSION   DAOSCH010000185 DAOSCH010000000
VERSION     DAOSCH010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32216_length_2219_cov_6.748614"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="PLZ54_09005"
     CDS             <1..409
                     /locus_tag="PLZ54_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOJ67097.1"
                     /translation="FPDYYGIDMARMNEFCAFRAAVQLLKDSGRQKIMDDIYEKCKQQ
                     ENWPKEKVTNYVKEIYAPFTQEEISSQIAKMLTPDEVDCPVELVYQSIDNLHKACPQH
                     KGDWYFTGDYPTPGGNRMVNKAFIDYYEGKDKN"
     gene            complement(422..1444)
                     /locus_tag="PLZ54_09010"
     CDS             complement(422..1444)
                     /locus_tag="PLZ54_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOJ67098.1"
                     /translation="MQPLIEAVDKEVLKRELTPEKFLRPTNKSDNEIYVITAHNSPNV
                     MLEIGRLREMAFRSWGGGTGKGIDVDAYDYSEKPYYQLIVWDPEENEIVGGYRFLFGK
                     DVPLNEKGEPEFVMSHLFKYSKKFINEYLPYTIELGRAFIQPAYQTSQKGSKSIFALD
                     NLWDGLGALIHLAKEARYFIGKVTMYHAYPAAARELIYEYMFRFFPDKEKLILPRNPV
                     TISNNIKILSNKLFNEKDKEANYKILQKMVRKENVNIPPLFNAYIGLTNTLRMFGSTI
                     DPNFSSVYETGIMLTMSDIVETKRKRYIEPYIEYLKNVFSNGTFSKINAYESHCRNIY
                     NFFQFE"
BASE COUNT          661 a          405 c          397 g          756 t
ORIGIN      
        1 cttccccgat tattacggta tcgacatggc ccgtatgaac gaattttgtg ctttcagagc
       61 tgcagttcaa ttgctaaaag acagcggcag gcagaaaatt atggacgata tttacgaaaa
      121 atgcaagcaa caggaaaact ggccgaaaga aaaagttacc aattatgtga aggaaattta
      181 cgctcctttc acgcaggaag aaatttcgtc tcaaattgcc aaaatgctta ctcccgatga
      241 ggtagattgt ccggtagagc tggtttatca atccatcgac aatttacaca aagcttgccc
      301 acagcacaaa ggcgactggt attttactgg cgattatccc acgccgggcg gcaacagaat
      361 ggttaacaag gcttttatcg actattacga aggaaaagat aaaaattaaa tattttagca
      421 gttactcgaa ctgaaaaaag ttataaatgt ttctgcagtg agattcgtaa gcgttaattt
      481 tggaaaacgt tccgtttgaa aacacatttt tcaaatactc aatgtaaggt tcaatatatc
      541 ttttgcgttt agtttccaca atatcggaca tggtcagcat tatgcccgtt tcgtaaaccg
      601 aactgaaatt tgggtcgata gtcgaaccaa acatgcggag tgtatttgtc aggccgatat
      661 atgcattgaa aagcggagga atgtttacgt tttcctttct caccatcttt tgtaaaattt
      721 tgtaatttgc ctctttgtct ttctcgttaa aaagtttgtt tgacaaaatt ttaatattgt
      781 tgctaatggt taccggattt cttggtaaaa tcagtttttc tttgtcgggg aaaaaacgaa
      841 acatatattc gtaaatcaac tcccttgcag cagccggata agcatgatac atggtaactt
      901 ttcctataaa atatcgggct tccttagcca aatgaataag agctcccaaa ccatcccaca
      961 aattatcgag tgcaaatatg ctctttgatc ctttttgaga ggtttgatag gctggctgaa
     1021 taaatgctct acctaattcg atagtataag gcagatattc gttgataaat tttttactgt
     1081 atttgaaaag atgcgacatt acaaattcgg gttctccttt ttcatttaac ggaacatcct
     1141 ttccaaataa aaaacggtaa ccgcccacaa tttcattttc ttccgggtcc caaacaatca
     1201 gttgataata gggtttttcc gaataatcat aagcatccac atcaattccc ttgccggttc
     1261 ccccacccca cgaacgaaaa gccatttcgc gcaaccgacc gatttccaac attacgttag
     1321 gagaattgtg tgcagtaatt acataaattt cattatctga tttattagta ggacgtaaaa
     1381 atttttcggg agtaagttct cttttcagaa cttctttatc gactgcttca ataagtggtt
     1441 gcataacaga attaaaaaaa atattagaga attgagtaat tattcaaacc tgttttctat
     1501 tttgaaattt ggattgaaca attttcttgt tgcaaaagta ctctttctct tccgtctgtt
     1561 caaattttgc atgtaatatt tgttttacat ttgtatgaaa atttttttta gttaaagata
     1621 ctgttttatt tcttgttatt ctattggcgg tcttttttaa gtataacagt tgattgtacc
     1681 aacagtaagg gattgggggc actttgctgt caagctacac cgaagttcga gcaaggtaga
     1741 atgcttgatt taccgctgaa acccttattg ctgcttcact ttgtatgcgg tgtttttctt
     1801 tgtcgttcac tcacattgtc aagttgcatt ttaataaatt caacggttga gttgtccgac
     1861 attgcattgt aaacccttgc tattaaatat ttttcagctt tttccattaa ctctaactcg
     1921 ttgccagaaa catataaggc gactttttca acgtttttat catacggagt agctttactg
     1981 tcaatatatt tctttgttgc tatccactat tttaaagtca tgatttgctg ttgtgttctc
     2041 tgatttgtta cgccaaataa tttctgcaaa atagtccagt taattagggg gctaagacat
     2101 ctctcgatgc cggacacggg aacaaatttc aattcctttt ggtgcgatta aaagtacaga
     2161 tataactgcc gtacgaaagt ttttaaaaat gttgtttttg aaaattcata tatttgtat
//
LOCUS       DAOSCH010000186         2171 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_33210_length_2171_cov_7.446597, whole genome shotgun sequence.
ACCESSION   DAOSCH010000186 DAOSCH010000000
VERSION     DAOSCH010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33210_length_2171_cov_7.446597"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            278..877
                     /locus_tag="PLZ54_09015"
     CDS             278..877
                     /locus_tag="PLZ54_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOJ67099.1"
                     /translation="MSSEIKLTITGLVYNQNIVGTYGLVLSEESGNRRFSVMIGEPEA
                     QSIALKLNNKKLPRPLTHDLIKTILDVFEAKLVKVLISDMINDVFYSELHILKDEKIL
                     VVDARTSDAVALAVRCNSPIYIKSDILDIVGVVVEENEIEETTDNEENIEELSDQELD
                     NLSLNELEELLSMAVNEEKYELAVHIRDAIQRKKTKEKF"
     gene            complement(1390..1965)
                     /locus_tag="PLZ54_09020"
     CDS             complement(1390..1965)
                     /locus_tag="PLZ54_09020"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOJ67100.1"
                     /translation="MEKLLQEEIRKAVEVLRQGGTILYPTDTVWGIGCDATSAEAVKK
                     VYEIKRRADSKALLVLVDSASRILSYVDEVPDMAWQLIEITDKPLTIIYPEAKNLAAN
                     LIAADRSIGIRVTRELFSKNLCEQFRKPIVSTSANISGNPTPSNFSQIDEAIKSAVDY
                     VVNWRQDDISEPKPSSIVKLSKGNVIQIIRE"
BASE COUNT          748 a          370 c          374 g          679 t
ORIGIN      
        1 aagccatgaa agccactcag gaaggcgttg ccgattactt gtcgcacaaa taaaaggtga
       61 gtgatcaact tataaaaagc cgcttaaatt actcaagcgg cttttttaat atacttttaa
      121 cctatttttg atgcaacatt aaagacagaa aaactatgta ttcatttttg gttatcgttt
      181 ttaaaaaaat tgtgtacctt tgtcataaag aaagaataac aattcgagtt taatgataaa
      241 agtcattttg tcaactaatt gcaactaaat agtctgtatg agttcagaaa taaaattaac
      301 gataacaggg ttggtttata atcaaaacat tgtgggtact tacggcttgg ttcttagtga
      361 agaatcgggc aatcgacgat tttccgtgat gattggagaa cccgaagcgc aatctatcgc
      421 attgaagctc aacaataaaa aacttccacg tccactcact cacgatctga taaaaactat
      481 tttagacgtt tttgaagcaa aactggtgaa agtgttgatt agcgacatga taaatgatgt
      541 tttttactct gaattgcata ttctcaaaga tgagaaaata ttggtggttg atgcacgaac
      601 ttcagatgct gtggcattag cggttagatg taacagtcca atatacatca aatcggatat
      661 tctggatatt gtaggtgttg ttgtggaaga aaacgaaata gaagaaacaa ccgacaacga
      721 agaaaatatc gaagagttat ccgatcagga attggacaac ctttcgctca acgaattgga
      781 agaattactt tctatggctg taaacgaaga aaaatatgaa ctggctgtac acatccgaga
      841 tgctatccaa cgcaaaaaaa caaaagaaaa gttttgattt tctcaaaata aatttgtttc
      901 aaccataagt tgatgactac ggaattgctt cactttatcg aattattgaa ttgaaaatta
      961 aaatactata aaccaaaagc aatgggtcaa tatcagcgga aagtttaatt ataatttttt
     1021 tctgttagta tgcatagcaa atgttattgt aaaattaatc aagaatgata ttatgaaatc
     1081 cacatgtatt gcatacacaa acacgaatga atataatatt cagacatgtg ggttccatca
     1141 gaccttataa aaaataaatt atattctgat gaaattaatg tacaacttgt gaacttttta
     1201 caacaaatta gacctggtta ttcaactatt ctttatcata tgaagaattt tttcgatatt
     1261 cgttggctat aaaaacttac aggaaaaagt catagtatca aagttctttc atcaaaataa
     1321 aaaatgtaac cttaatgtat ttagtataaa caacagaaaa agtacggatg caaaaaactt
     1381 tttgcgattt tattccctga taatttggat tacgtttcct ttactcaact tgacaatgga
     1441 agaaggtttg ggttcggaaa tgtcatcctg tcgccaattt accacataat cgacagccga
     1501 ttttatggct tcatctattt gcgaaaaatt tgacggcgtg gggttcccgc tgatattggc
     1561 agacgtggaa acaatgggtt ttctgaattg ttcacacaag ttttttgaaa acaattccct
     1621 tgttaccctg atgcctattg aacgatcagc agcaatcaaa ttggcagcca gatttttggc
     1681 ttcgggataa ataatggtga gaggtttatc ggtaatttcg atcaactgcc aagccatatc
     1741 gggcacctcg tccacataag aaagtattct cgaggcggaa tcaaccaaaa ctaataaggc
     1801 tttgctatcg gctcgccgct ttatttcgta aactttctta acggcctctg cgctggtggc
     1861 atcgcaacca attccccata ccgtatcggt gggataaagt atagtacctc cttgcctcaa
     1921 cacttcaaca gcttttctga tttcttcctg taataatttt tccattttgt aatgatagtt
     1981 taatccaaac cttcaatgaa gactgcaagc aaaaataaca aaaatacgcc ttacttcaaa
     2041 aaaggataaa ttattccacc caagttttac ttctaccgaa actgagattt cctaacaatt
     2101 tctgcaaatt ggttaatttt tatcactgcc gtacgaaagt ttttaaaaat gttgtttttg
     2161 aaaattcata t
//
LOCUS       DAOSCH010000187         2160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_33464_length_2160_cov_6.723990, whole genome shotgun sequence.
ACCESSION   DAOSCH010000187 DAOSCH010000000
VERSION     DAOSCH010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33464_length_2160_cov_6.723990"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..126
                     /locus_tag="PLZ54_09025"
     CDS             4..126
                     /locus_tag="PLZ54_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dna2/Cas4 domain-containing protein"
                     /protein_id="HOJ67101.1"
                     /translation="MEKEITEIIQSDDCPPVIHSKICKNCSYYDFCYVEEKEDS"
     gene            217..594
                     /locus_tag="PLZ54_09030"
     CDS             217..594
                     /locus_tag="PLZ54_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOJ67102.1"
                     /translation="MKEYNDIFRERTKKLALEIVREMVIVKFSDEVSVIRKQIIRSAT
                     SVAANYRAVCRARSERERFSKMCIVVEETDETAFWLELIEELGMLPNAKVEEFKSETE
                     EILKVMSSYKKKLAKTLQITHHP"
     gene            613..1629
                     /gene="cas1b"
                     /locus_tag="PLZ54_09035"
     CDS             613..1629
                     /gene="cas1b"
                     /locus_tag="PLZ54_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HOJ67103.1"
                     /translation="MKKTYYLFNPGTLQRKDNTIKFTPIEEDENGIDHEGQPRYLPVE
                     DISEFYVFGALTANSALYNFLGQHDIAVHFFDYYENYTGSFMPRDSLLSGKMILAQTS
                     AYQNKKKRLELGRKFVEGAAFNMSKNLKYYSNRGKNLEENIEKIEQYVAMLPQTQAID
                     EIMGLEGNIRQTYYDGFDEIINDFSMGGRSKQPPRNEVNALISFGNMMCYSQCLRAIH
                     QTQLNPTISYLHTPGERRYSLSLDISEIFKPILVDRVIFKLLNKRELQEKHFESKLNK
                     CLLNPSGKKIFVQAFEERLNETIQHRSLKRKVSYRHLIKLECYKLSKHLLGMEEYKPF
                     KMYW"
     gene            1630..1893
                     /gene="cas2"
                     /locus_tag="PLZ54_09040"
     CDS             1630..1893
                     /gene="cas2"
                     /locus_tag="PLZ54_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOJ67104.1"
                     /translation="MYVILVYDVNEKRVAKMLKLCRRYLNWIQNSVFEGEISEVKLKE
                     LIIFAERIMKLEEDSLIIFSSRTEKFLDKQIIGKERSSTDIFL"
BASE COUNT          771 a          314 c          431 g          644 t
ORIGIN      
        1 gaaatggaaa aggagattac ggaaattatt caatccgatg attgtcctcc ggtgattcat
       61 tcgaaaattt gtaagaattg cagctactac gatttttgtt atgtggaaga gaaagaagat
      121 agctgaaaaa ttggcgaaag gaataagcga tgggtgataa gtgataaatt gataaagtga
      181 taggtaataa actggtgata aaaattattg tttggtatga aggaatacaa tgatattttc
      241 agggaacgaa caaaaaagtt agcattggaa attgtaagag aaatggttat agtgaaattt
      301 tcggatgaag tttcagttat caggaaacaa attattcgtt ctgccacttc tgttgcagcc
      361 aattacaggg cagtgtgcag agctcgatcg gaacgggaac ggttctccaa aatgtgcatt
      421 gttgtagaag aaactgatga aaccgctttt tggctggaat taatcgaaga actcggaatg
      481 cttcctaatg ctaaagttga ggaatttaaa agtgaaaccg aagaaattct gaaagtcatg
      541 tcttcttaca aaaagaaatt agcaaaaaca ctgcaaatca cccatcaccc ataaatctat
      601 cacctatatt ttatgaaaaa aacttattat cttttcaatc cgggcacctt gcagcgaaag
      661 gacaatacca ttaagtttac accgattgag gaagatgaaa atggcattga tcacgaaggt
      721 caaccacgtt atttgccggt agaagatatt tcagaatttt acgtgttcgg tgctttgact
      781 gccaatagtg ctctgtataa ctttctggga caacatgata tagccgtaca tttttttgat
      841 tattatgaaa attatacggg ctcattcatg ccccgcgaca gtttgttgtc tggaaaaatg
      901 attctggcac aaacatcagc ttatcaaaat aagaaaaaac gattagagct gggtcgtaaa
      961 tttgtagaag gagccgcatt caacatgtca aaaaatttga aatattatag caatcgagga
     1021 aaaaatctgg aagaaaatat tgaaaaaata gaacagtatg tggctatgtt accacaaact
     1081 caagcaattg acgaaatcat gggactggaa ggcaatatcc gacaaactta ttatgatggt
     1141 tttgatgaaa ttatcaacga tttcagcatg ggaggaagaa gtaagcagcc accgagaaat
     1201 gaagtgaatg ccttgatttc gtttggcaat atgatgtgtt attcccaatg tttgcgtgct
     1261 attcatcaaa cacagttgaa tccaactatc agctatttgc atactccagg agaacgtcgt
     1321 tattccttat cattggatat ttcggaaatt tttaagccca tactggtgga cagagtaatt
     1381 ttcaaattgt tgaacaaaag agagcttcag gaaaaacatt ttgaaagtaa actcaacaaa
     1441 tgtttgctta atccatcggg gaaaaagatt tttgttcaag cattcgaaga acggctcaac
     1501 gaaaccattc agcaccgatc gctcaaacgg aaagtgagtt atcgacatct catcaagttg
     1561 gagtgttata aactaagcaa acatttgctg gggatggaag aatacaaacc gtttaaaatg
     1621 tattggtaaa tgtacgtaat tttggtatat gacgtcaatg aaaaacgagt agcaaaaatg
     1681 ctcaaacttt gtcggagata tttgaactgg atacaaaatt cggtttttga aggagaaatt
     1741 tcagaagtca aattgaaaga attaattata tttgcagaaa gaatcatgaa acttgaagaa
     1801 gattcattga ttattttttc cagcaggaca gaaaaatttc ttgacaaaca aatcattgga
     1861 aaagaaagaa gtagcactga tatttttttg taatcaattg tcgaaagatg agttttttct
     1921 ttttatgttg ttgaattcga atatgatttt ttttttgatc tattgaaaat caagataaaa
     1981 acaagttgtc gaaaatcaag gatttttata tttttgccta tcgacaattt ttacattcca
     2041 taaaggatgc tcattgtttt gctaacaatt tgatttttag ataaaatttt tgatacagag
     2101 cacggctttt aatcgcacca gaaggaattg aaatgctcgt acttcaaagg agtaaatatc
//
LOCUS       DAOSCH010000188         2133 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_34149_length_2133_cov_6.585178, whole genome shotgun sequence.
ACCESSION   DAOSCH010000188 DAOSCH010000000
VERSION     DAOSCH010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34149_length_2133_cov_6.585178"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1122)
                     /locus_tag="PLZ54_09045"
     CDS             complement(<1..1122)
                     /locus_tag="PLZ54_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOJ67105.1"
                     /translation="MGKIIGIDLGTTNSCVAVMEGNQPIVIPNNEGKRTTPSVVAFLE
                     NGERKVGDPAKRQAIINPTRTVYSIKRFMGRSYDEVTNEIARVPFKVVRGENNIPKVD
                     IDGHTYSPQEISAIILQKMKQTAEDYLGTQVTDAIITVPAYFNDAQRKATKEAGEIAG
                     LNVRRIINEPTASALAYGLDKTNREMKIVVFDCGGGTHDVSVLELGDGVFEVKATNGD
                     THLGGDDFDQRIIEWLVQEFKNENSGVDLTKDPMALQRLKEAAEKAKIELSTATSTEI
                     NLPYIMPVDGVPRHLVRTLTRAKFEQLVDDLVQRTIEPCRTALQKAGLTVNDINEVIL
                     VGGSTRIPAIQQAVEKFFGKAPSKGVNPDEVVAIGAAIQG"
BASE COUNT          621 a          456 c          349 g          707 t
ORIGIN      
        1 tccttgaatg gcagcgccaa ttgctacaac ttcatcggga tttacacctt ttgacggagc
       61 ttttccaaag aatttttcaa cagcctgctg aatagccgga atacgggttg aaccaccaac
      121 caaaatcact tcattgatgt cgttcacagt caaacccgct ttttgaagtg cagtacgaca
      181 aggttcgatt gttctttgaa ccaaatcatc aaccagttgt tcaaatttgg cgcgggtcaa
      241 tgtacgaacc aaatggcgag gaacaccatc gacaggcatg atatatggca aattgatttc
      301 ggtagaagta gctgtagata attcaatttt tgctttttct gcagcttctt tcaaacgttg
      361 caatgccatt ggatctttgg ttaaatctac gccactgttt tcgtttttga attcctgaac
      421 cagccattcg atgattcttt ggtcaaaatc gtcacctccg aggtgagtat ctccattggt
      481 ggctttcact tcaaatacac catctcccaa ttccaatacc gaaacatcat gcgttccacc
      541 accgcagtca aacaccacaa ttttcatttc ccggttggtt ttgtctaatc cgtaagccaa
      601 agcagaagcc gtaggttcat taataattcg acgaacattt aacccggcaa tttctcctgc
      661 ttcttttgtt gctttacgct gtgcatcatt aaaataagcc ggaacggtaa taatggcatc
      721 agtaacctga gttcccaaat aatcttcggc agtttgtttc attttttgaa gaatgatagc
      781 cgaaatttct tgcggagagt aggtatgacc gtcaatatct actttcggta tgttgttttc
      841 tcctctcact actttgaaag gtactctcgc tatttcattg gttacttcat cataactgcg
      901 ccccataaaa cgcttaatgg aatatactgt tcgggtagga ttaatgatgg cctgacgttt
      961 tgcaggatct ccaacttttc tttcaccatt ttccaaaaaa gctactaccg atggagtagt
     1021 tctttttcct tcattatttg gtatcacaat aggttgattt ccctccatca ctgcaacaca
     1081 agagttggta gtacccaagt cgattccaat aatttttccc atgactttat tgtttattag
     1141 ttttttttaa tcagttaatt tcaatttttt ctcccttagt ttcacaaagg ttgtgccaac
     1201 agaatattga cacacaaaat tgaatttttt ttgaaaaaaa ctgaaaaaaa ttttatttct
     1261 ggaacaaaaa ctgacaaaag ggcagaaatt ttgacaagca gtggattgaa aaataaagaa
     1321 attccttaaa attgactatt ctcactgcaa gcagcgaggt attaagccaa ttttatggct
     1381 ttgccaccgg aatttccaaa aaaataaaaa caaaagctac cccgacgcaa gcatcgggga
     1441 attcttattg attaattttt tttgtttcga tattttaaat aatttatgat atattacaag
     1501 tttaaattga aacattataa cattctcact aagccttcta attctaaaag cgataggttt
     1561 tgtttattca ttaaacccga atcagctaca atcacaaaat cgaccaaatt aaatcggttg
     1621 acaaaatcct ccacaaccgg aagcatagtc cgcccttcgt actgcgaacc attgaaaatt
     1681 gaataagaaa gcggatatca gtctttactt accaaaagtt ctaaaactac ttgtggttgt
     1741 gcgtgcttgc cgtctttaga gaattcgatt tactcatcgg ttggctcaga cgagctacca
     1801 ccaagtgtct caggatttca tcctctatcg catcgaaccc gatagcttga tataccctat
     1861 ccaatatcaa ttgagtactg ttgagcagaa tgttttctat gttcgtgaga agatattctg
     1921 taaactgttt ctcttctgct tcttgatcag attgctcaaa aatatcccga tctcccaaat
     1981 aagcagaaat tcatttcttg ccttgaagaa taagtttttc tatttcatct ttatcttcac
     2041 ttacacctat tgtttttagt tctctaaaaa ctcctttcct tttctcggct acaacgacac
     2101 tggtagtccc tgaacgattc tttttcttac gta
//
LOCUS       DAOSCH010000189         2081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_35367_length_2081_cov_5.488648, whole genome shotgun sequence.
ACCESSION   DAOSCH010000189 DAOSCH010000000
VERSION     DAOSCH010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35367_length_2081_cov_5.488648"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(555..2000)
                     /gene="amrB"
                     /locus_tag="PLZ54_09050"
     CDS             complement(555..2000)
                     /gene="amrB"
                     /locus_tag="PLZ54_09050"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOJ67106.1"
                     /translation="MEKFNETIDRRPAVAGKFYPANVEELQKEIDIFFKNAAPKQLDR
                     VRAVICPHAGYVFSGKITASAFNQIDANREYKRIFLIGSSHYKYFDKASVYCSGNFLM
                     PYGEEKVDMDFGRKLVENFPEWFTDDRTPHLNEHCLEVQLPFLHYVLRKNYVIVPILI
                     GTAHPSVCKQIASVLKPYFNEENLFVVSSDFSHYPAYQDAKYVDSLTKDAICSNRPEK
                     LLETLAENERKHISNLQTSLCGWTSVLTLLYMTSSNKSLKFHAVDYCNSGDVKYYGER
                     DRVVGYWAIAVAESKHAAEDFYLTEKDKKDLLKISRKTLEEHCRAGRVSEVDTATLSE
                     TVKKNCGAFVTLHKRGALRGCIGILTAEKPLYKTVQEMTIAAASHDPRFMPVEASELP
                     FIDIEISALSPLKKINDISEIELGKHGILIEKNFHRGVFLPQVATETGWSKEEFLGHC
                     SADKAGLGWDGWKTADIYIFTATVFSEKDFW"
BASE COUNT          669 a          417 c          374 g          621 t
ORIGIN      
        1 agcaataatt aaaattcaat aaaatggcag aacaaaaaca agtacaatca aaaacagcaa
       61 tttaaggacg atcaaacttt gaaaaaaata attttatgtt agctgcttgc cacacgatgc
      121 aattgtgagg cagaagagtt gtatgagatt tttgaactaa acattaaaaa atttgattat
      181 tctatctctc acacagttat tgaaaagctg aaagcatttt tcaacaattc agtagcgcac
      241 aaggataaaa actttgggaa tgctcgcttt gtcttctttg aaaaaacaac tgttgaaaaa
      301 gtaaaattac acacagcgag ttcaaaacct tatccttcaa gcaaacctta gtaaccaaca
      361 atatgtccac atgaatcaaa ccttaaaggg ttgcttcgga tgctacatcc tcgcgaggag
      421 aggactatta aaagtaggaa taaaggattg ttgaataatg ttttgatcga ataattattt
      481 cttgctattc aaacaaaaca caaataaata cttgattttc agtttaccaa agctttgcca
      541 aaattattct atttttacca aaaatctttt tcgctgaaaa cggtagccgt aaaaatgtaa
      601 atatcggctg ttttccagcc atcccaaccc aaacctgcct tgtcggccga acagtgtccg
      661 agaaattctt ccttactcca gcctgtttct gtagctactt gaggcagaaa tacgcctctg
      721 tgaaaatttt tctcgattaa aattccgtgt ttccccaatt cgatttcgga aatatcgttg
      781 atttttttca ggggcgacaa agccgagatt tcaatatcga taaaaggcag ttcggatgct
      841 tcgaccggca taaatcgcgg atcatgggaa gcagcggcta tcgtcatttc ctgaacggtt
      901 ttgtacaagg gcttttcggc tgtcaaaata ccgatacaac ctcttaaagc tcctctttta
      961 tgcaatgtta caaaggcacc gcaatttttt ttaacggttt ccgacaaagt tgccgtatcc
     1021 acttccgaaa ctcttccggc tcgacaatgt tcttcaagcg tttttctcga aattttcagc
     1081 aggtcttttt tgtctttttc agtgagataa aaatcttcgg cagcatgttt actttcggct
     1141 actgcaatag cccaataacc cacaaccctg tcccgctcgc cataatactt cacatccccc
     1201 gaattgcaat aatccaccgc gtggaacttt agcgatttgt ttgaagaagt catataaagc
     1261 agtgtcaata ccgaagtcca accgcacaaa ctggtctgaa gattagaaat atgttttctt
     1321 tcattttcag ccaaagtttc caacaatttt tcgggacgat tgctacaaat ggcatctttg
     1381 gtgagggaat caacatattt ggcatcctga taggccggat agtgagaaaa atcggaactg
     1441 acaacgaata aattctcctc gttgaaataa ggttttaata ccgatgctat ttgcttgcaa
     1501 accgaaggat gggctgtgcc gataaggata ggaacgataa cataattttt tcttaacaca
     1561 tagtgcaaaa atggaagttg aacttccaga caatgttcat ttaaatgagg agtacggtcg
     1621 tcggtaaacc attctggaaa attttccacc agttttcgtc caaaatccat atcgactttt
     1681 tcctcaccgt aaggcattag aaaattgcct gaacagtaaa cggaagcctt gtcaaaatac
     1741 ttgtaatgag aggaaccaat aagaaaaatt cttttgtatt ccctgtttgc atctatctgg
     1801 ttaaatgccg aagcggtgat tttgccggaa aaaacataac cggcatgcgg acaaataacg
     1861 gctctgaccc tgtcgagctg tttgggtgca gcatttttga aaaaaatatc gatttctttc
     1921 tgcaactctt caacatttgc cggataaaac ttgccggcta ctgcaggcct tctgtcgatg
     1981 gtttcgttaa atttttccat aatatcaagt caaggttaag tgattgattt actatgaaaa
     2041 acaattcgta tttaaaaaag ttcaaaggaa attcaaatat a
//
LOCUS       DAOSCH010000190         2002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_37386_length_2002_cov_5.500257, whole genome shotgun sequence.
ACCESSION   DAOSCH010000190 DAOSCH010000000
VERSION     DAOSCH010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37386_length_2002_cov_5.500257"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1850)
                     /locus_tag="PLZ54_09055"
     CDS             complement(6..1850)
                     /locus_tag="PLZ54_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOJ67107.1"
                     /translation="MEFNIFRQGKAVVLFLFYFCAIAAKAQNGYQIEKITIVGNQTFS
                     KSELLEKTNLATYNFLQRTFLKKEPVIFNENLMELDIERMTRFYQTEGFPFVEVQVDS
                     LATNHKKQKVEIYIKIEENTPIFFGEIGFDIESQSISEEKKDTLLHLIKKHIVSKNGR
                     RFRDKDVHEDKSRIEQIFINSGYVYVQVDHLINLSLDSYRADVVYVIRPFEICFFGKT
                     EIVGNQNIKESLIRRQLAYTEGEIYSNKKLDKTRKQLFDLQLFRVASLQPQIDASSTR
                     NSIPVKLTIEETPQWNAQIGLGYGTEDQFRTFLDLSFHSLWGDASRFHLYAKHSALEP
                     YHFSLSWIQPQLFSKNISMRLNPFLRRQAEPGFDIRTYGLNLPFGYSFSDKINLTLSY
                     YLENIKQYSEVDNLELINPESKDFLYNKSGLLTTFQYNNAFPQINPERGEIMSIGVKS
                     NGYLFGSDFDYTKIWIDTRKYLQINNFSCAARLMGGTVFSSNVIEYVPVEDRFYSGGS
                     NSVRGWSRWALGPKRDDGSPLGGKSNLELSLEIRHPLFWLLESAVFLDAGNVWASSFH
                     FPLDKLAYSAGTGLRIKTPVGAIRLDISVPLWNEKKSVQVFLNVGQAF"
BASE COUNT          625 a          370 c          360 g          647 t
ORIGIN      
        1 ggtttttaaa atgcttgtcc gacattcaga aaaacttgta ccgatttttt ttcgttccaa
       61 agcggaacgc ttatatccaa gcgaatagcg ccaacaggtg ttttaattct cagtcctgta
      121 cctgctgagt aagccagttt atccaaagga aaatggaatg aggatgccca cacattaccg
      181 gcatccaaaa acacggcact ttcaagaagc caaaataaag gatgtcgtat ttccaaactg
      241 agctccaaat tgctttttcc tcctaatgga gaaccgtcat ctcttttggg accaagagcc
      301 cagcggctcc agcctctaac agagttgctc cctccagaat aaaatctgtc ttccactgga
      361 acatattcta ttacattgga ggaaaaaaca gttccgccca ttaatcgtgc agcacaagaa
      421 aagttgttaa tttgcagata ttttcgtgta tctatccaaa tttttgtata atcaaaatca
      481 ctcccaaata aatatccatt gctttttacg ccaatactca taatttcccc acgttcaggg
      541 ttaatttgcg ggaaagcatt gttgtattga aaagtagtca gcaatcccga tttgttgtaa
      601 agaaaatctt tactttcagg attgattaat tccaaattgt ccacttcgga gtattgttta
      661 atattttcca aataataact taaggtaaga tttattttat cggaaaagga ataaccgaat
      721 ggtaaattta gtccataagt tcgaatatca aaaccgggtt ctgcctgcct gcgtaaaaaa
      781 gggttaagcc gcattgaaat atttttagaa aacaattgcg gttgtatcca gcttaaactg
      841 aaatgatacg gttccaatgc cgaatgtttg gcgtacaaat gaaatcgtga agcatcgccc
      901 cacaacgaat gaaaggacag atcaagaaag gtacgaaatt gatcttctgt accatagcca
      961 agtccgattt gagcattcca ttggggagtt tcttcaatgg ttaattttac tggaatggaa
     1021 tttcgagtgg aggaagcatc aatctgaggt tggagcgagg ccaccctgaa aagttgtaaa
     1081 tcgaaaagtt gttttcgtgt tttatccagt ttcttattgc tgtaaatctc tccttctgtg
     1141 taggcgagtt gacggcgaat caaactttct tttatgtttt gattgccaac aatttcggtc
     1201 ttcccaaaaa aacaaatttc gaatggtcga ataacataaa cgacatctgc ccgatatgaa
     1261 tcgagtgaaa gattaatcaa atgatcaact tgtacatata catatcctga gttgataaat
     1321 atttgttcga tgcgggattt gtcttcatgc acatctttgt ctctgaaacg cctgccgttt
     1381 ttagatacaa tgtgtttttt tataagatgt aatagtgtat cctttttttc ttccgaaata
     1441 gattggcttt ctatgtcgaa tccgatttct ccaaaaaata tcggggtgtt ttcttcgatt
     1501 ttaatataaa tttcgacttt ttgtttttta tgattggtgg ccaatgaatc cacttgcact
     1561 tcaacaaacg ggaaaccttc ggtttgataa aaacgggtca ttcgttcaat atccaactcc
     1621 attaaatttt cgttaaaaat taccggttcc tttttcaaaa aagtacgttg caaaaaattg
     1681 taagtagcaa ggttggtttt ttccagtaat tccgattttg aaaatgtttg attgccgact
     1741 atcgttattt tttcaatttg atatccgttt tgagctttcg cggcaatggc acaaaagtaa
     1801 aacagaaata aaacgacagc ttttccctgc cgaaaaatat taaattccaa aaatttgaat
     1861 cgatacggca ttcctatgtc ctaaaaattt aaacatcgag gttttgcaga cgtgatgaca
     1921 aaaaaaaatg ctgtttaatt gggaaaataa tttcatcaga atataattta ttttttaaag
     1981 gtctgatgga acccacatgt ct
//
LOCUS       DAOSCH010000191         1961 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_38526_length_1961_cov_11.749213, whole genome shotgun
            sequence.
ACCESSION   DAOSCH010000191 DAOSCH010000000
VERSION     DAOSCH010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38526_length_1961_cov_11.749213"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            419..1780
                     /locus_tag="PLZ54_09060"
     CDS             419..1780
                     /locus_tag="PLZ54_09060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOJ67108.1"
                     /translation="MAFVVIMPKQGQSVESCIVTEIKKKKGDAVKKGDILFSYETDKA
                     SFEEESPVDGVVLECFHNEGDEVPVLENMMVIGQPGEDYSALLGESRQAEGDREEKKS
                     SSPSLEIKHVEVNAQTQKTEISVTDARIMPASAFISPRAKRLAEKEAVNVLALSGSGP
                     KGRIIEKDVKAALKNCPKMTPLAKKIASEEGLQPQKAGTGLAGTVRALDLAASANPVY
                     VIDFEDRKISNVRKIIARSMHASLQNSAQLTHHLGADARRILELRKHVKAAMETGTLT
                     TNITLNDMVCFAVIKALKKFPNVNTHFLGDTIRYFKKVHLALAVDTDRGLMVPVVRNA
                     DDLSIEGLTNQLKEVAAACRKGSINPELLSAEAGTFTVSNLGNYGVEMFTPVINLPQT
                     AILGVNTIVPRPKDLGDGVYAFVPYIGLSLTYDHRALDGGEATRFLKQIAVEIETLEL
                     DIF"
     gene            1826..>1961
                     /locus_tag="PLZ54_09065"
     CDS             1826..>1961
                     /locus_tag="PLZ54_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOJ67109.1"
                     /translation="MFDLLIIGGGPAGYVAAERAGHKGLKVLLFEKKAMGGVCLNEGC
                     I"
BASE COUNT          632 a          323 c          452 g          554 t
ORIGIN      
        1 actccgattt cagatcaaga atttataata ggtcttcttg cggataatca ttaaataaat
       61 ctaattgact ttagatttct gactttctat tattttcaat gtaattttta ttttgataga
      121 atcttgaaat ataataatat atgcaaatat caaagcattt ttcaagcatt tttacacttt
      181 tttatgaaaa taaaaaataa actaactttg aaagactttt gggtgagtct caaaaataaa
      241 aaatttgtga aaaataagat gaagaaaaaa tgaattaaaa acaatattgt ttgacttttt
      301 gaaagagatg ttttgaaaaa aaatcaaata ttgcagtata aaaatcaaat attgcatttt
      361 taaaaaggca taaaataagt atacttgcac ataacattca tattcaaatt gtaaagttat
      421 ggcatttgtg gtaattatgc ccaaacaggg gcaatcggtg gaaagttgca ttgttaccga
      481 aataaaaaag aaaaaagggg atgccgtgaa aaaaggagat atcctgtttt cttatgaaac
      541 agataaagca tcttttgagg aggaatctcc tgtggatggc gttgtgttgg aatgtttcca
      601 taacgagggg gacgaggttc ctgtgttgga aaacatgatg gtgatcggac aaccgggaga
      661 agattattcg gctttattgg gtgaaagtcg gcaggcagaa ggtgatcggg aagaaaaaaa
      721 atcttcttcg ccttcgttgg agattaagca tgttgaggtc aatgctcaga cacaaaaaac
      781 ggaaatcagc gttactgatg ctcgaattat gccggcgtca gcgtttattt ctcctcgagc
      841 taaaaggttg gcagaaaaag aggctgtaaa tgttttggct cttagtggtt cgggaccgaa
      901 ggggcgaatt atcgaaaagg acgtaaaagc tgcgttgaag aactgtccaa aaatgacacc
      961 gctggcaaaa aagatcgctt cggaagaagg tttgcaaccc caaaaagccg ggacgggatt
     1021 ggcaggtaca gtacgtgcat tggatcttgc tgcatctgcg aatccggttt atgtaatcga
     1081 ttttgaagac agaaaaatat ccaatgtacg gaaaattatc gctcgttcca tgcatgcttc
     1141 gttgcagaat tcggcacagc tcacgcacca tcttggtgcc gatgcgcgta ggattctcga
     1201 acttcgcaaa catgtgaaag cagcgatgga aaccggaacg cttaccacca acattacatt
     1261 gaacgatatg gtgtgttttg ccgtgatcaa agcactgaaa aaatttccga atgtcaatac
     1321 gcattttctt ggcgacacga tccgctattt caaaaaggta catttggctc ttgcggtgga
     1381 taccgacagg ggattgatgg tacctgtagt tcgcaacgcc gacgatttat ccatcgaagg
     1441 tttgacaaat caattgaaag aggttgctgc agcatgccgc aaagggagta tcaatcccga
     1501 actactgtct gctgaagccg gtacatttac ggtgtccaat ttgggtaatt acggagtgga
     1561 aatgtttact cccgttatca atttgcccca aacagctata ttgggcgtaa atacaatcgt
     1621 tccccggccg aaagatttgg gcgacggtgt atatgctttt gttccttata tcggattgag
     1681 cctgacttac gatcatcggg cactggatgg tggagaggct acccgttttc tgaaacaaat
     1741 cgcagtggaa atagaaaccc tcgaattgga tattttttga agaaaacagt aaaatcgtaa
     1801 atgtaattaa ataaaataaa atgctatgtt cgatttattg ataataggag gaggacctgc
     1861 aggctatgtg gctgccgaac gcgcaggaca taaaggtctt aaggtgcttt tgttcgaaaa
     1921 gaaagcgatg gggggtgttt gtttgaatga aggttgcatt c
//
LOCUS       DAOSCH010000192         1929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_39500_length_1929_cov_4.906083, whole genome shotgun sequence.
ACCESSION   DAOSCH010000192 DAOSCH010000000
VERSION     DAOSCH010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39500_length_1929_cov_4.906083"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="PLZ54_09070"
     CDS             <1..1468
                     /locus_tag="PLZ54_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOJ67110.1"
                     /translation="TPVTFQGEKVVGNNKTISYKAGGSYTINASFNYVPEMAKSELYL
                     DFNVTVKKKTYQIPSVKVADGVIATAELVAVNASEMNAAVIPDKFQRIIKETQNADIK
                     FLIERWDLRPNELKKDEVVSLVEKIKEVKDAQNKQVSSFEILGYASPDGPIDLNTNLA
                     EKRQDVTSDYINKQMKKLKTSVKIDSKFTPEDWAGFQALMEKSNIQDKDVILRVLSMY
                     TDPEERERQIKNLSVAYTKIAEEILPELRRSQLKLTVDVIGKSDAEIMQLASNDPSKL
                     NVEELLYAATLTNDANAKASIYKKVTELYPNCVRGFNNLGAVYFQQGKLDDASAMFTK
                     AMQLDPNSPDVNYNCALIALAKKDLSKAEELLGKAAGTSGDLSKALGTLYIMKGDYAK
                     AKTSFKTNDVNNNVALLQILSGDYNSARKTLSAIQSPNATTAYLSAIIGARTNDRETV
                     YSNLKLAISKDKSMAAKALNDIEFAKFATDQTFLSIVQ"
     gene            complement(1582..>1929)
                     /locus_tag="PLZ54_09075"
     CDS             complement(1582..>1929)
                     /locus_tag="PLZ54_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOJ67111.1"
                     /translation="GISAALSMAASDYLSTKAEGDTRAAKSAAYTGVTYFLTVILLIL
                     PFLLMPNKFGALALTLSIAVLIIFCFNYYISVAKNLSFKPRFIEMTTISLGVAAFSFF
                     IGYLLNQILGVNV"
BASE COUNT          659 a          376 c          375 g          519 t
ORIGIN      
        1 tacaccggta acttttcagg gagaaaaagt tgtaggcaac aacaaaacca tttcctacaa
       61 agccggcggt tcctacacta tcaacgcttc cttcaactat gtgcctgaaa tggccaaatc
      121 ggaactctat ctcgacttca atgttaccgt aaaaaagaaa acgtatcaaa ttccaagtgt
      181 taaagttgcc gatggagtga ttgccactgc agaattggtt gcagtgaatg cttcagaaat
      241 gaatgcagct gtaattcccg acaagtttca acgtatcatt aaggaaacac agaatgccga
      301 cattaaattc ttgattgaaa gatgggatct tcgtcccaat gaattaaaaa aggatgaagt
      361 agtttctttg gtagagaaaa taaaagaagt gaaagatgcg cagaataaac aagtttcttc
      421 gtttgaaatt ttgggttatg cttctcctga tggcccgatt gatttgaata ccaatttggc
      481 agaaaaacgt caggatgtaa cttccgatta cattaacaaa caaatgaaga aactcaaaac
      541 ttctgtaaaa atagactcca aatttactcc tgaagactgg gccggcttcc aagctttgat
      601 ggaaaaatcc aatattcaag ataaagatgt aattcttcgt gttttgtcca tgtacaccga
      661 tccggaagaa cgtgaacgtc agattaaaaa tctgtcggtt gcctatacaa aaattgccga
      721 agaaatttta ccggaacttc gtcgttcaca gttaaaactc acggttgacg ttattggcaa
      781 atcggatgcc gaaattatgc aattggcttc aaatgatcct tccaagctga atgtagaaga
      841 attgctttat gcagccacac tgaccaatga tgccaatgca aaagcttcca tctacaaaaa
      901 agttaccgaa ctttatccaa actgcgttcg tggtttcaac aatttgggag ccgtttactt
      961 tcaacaagga aaactggatg acgcttctgc catgtttaca aaagccatgc agcttgatcc
     1021 aaattctccc gacgtaaatt ataattgtgc actcattgca ctggctaaaa aagacctttc
     1081 caaagctgaa gaattattgg gaaaagccgc aggcacttcc ggcgacttga gcaaagcatt
     1141 gggaacactt tatattatga aaggtgatta tgcaaaagct aaaacttctt tcaaaaccaa
     1201 tgatgtgaac aataatgtgg ctttgcttca aattttgagt ggcgattaca attcggctcg
     1261 taaaaccctg tcggctattc agtctccgaa tgctaccacc gcatatttga gcgcaattat
     1321 tggagcccgt accaatgaca gagaaacagt ttattcgaat cttaaactgg ctatcagcaa
     1381 agataaatca atggccgcaa aagctttgaa cgacatcgaa tttgccaagt ttgcaaccga
     1441 tcaaactttc ctctcgattg ttcaataatt gaattcataa cctattaaaa aaggggatgc
     1501 ttcagaagag gcattccctt tttttacgaa tatagacatg gttttgctta cggtttatct
     1561 cgaaaagcga gtaaatgaat tttaaacatt tacccctaaa atttgattca gcagataacc
     1621 gataaagaaa gaaaaagcag caacgcccaa gctgatggtt gtcatttcga tgaaacgtgg
     1681 cttgaaagaa agattttttg ctacagaaat ataataatta aaacagaaaa taattaaaac
     1741 ggcaatggaa agcgttagcg ctaaggctcc aaatttgttg ggcatcagca aaaatggaag
     1801 gattagcaaa attaccgtca aaaaataggt aacgccggta tatgctgccg actttgctgc
     1861 acgagtatct ccttcggctt ttgtagaaag ataatcggaa gcagccatag aaagcgcagc
     1921 agaaattcc
//
LOCUS       DAOSCH010000193         1922 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_39721_length_1922_cov_9.074451, whole genome shotgun sequence.
ACCESSION   DAOSCH010000193 DAOSCH010000000
VERSION     DAOSCH010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39721_length_1922_cov_9.074451"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..1102
                     /locus_tag="PLZ54_09080"
     CDS             116..1102
                     /locus_tag="PLZ54_09080"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOJ67112.1"
                     /translation="MKNFKIGGISILLAAIFTSCAETDFDAIPVNKLMGDSLTTTYTI
                     EQLKNNFLTLNDAYSDTVNYKGGLYTADPIQAESDIVISGYITSTDVDGNVYKYFVVQ
                     EPGANGQAIKVSIDASGLSAIYPLGQKVWIRCNGLYIGRYGEAEQIGSRYVNKERFKV
                     KKSTGDTIYRIEPGRMPFLIANEHIHAYGMPDPTVIKPDTMTIAQIKASNHTKLVNKL
                     VCIKNAFFTGKSEGDNLTDSELIFAPSTNGAGYPQLRDINDGTGEISIATSEYAKFAN
                     YPLPASTYRGDITVIIGWYRNYTNAAGAWQLTLRTINDLGKGFESYKAKIQK"
     gene            1129..>1922
                     /locus_tag="PLZ54_09085"
     CDS             1129..>1922
                     /locus_tag="PLZ54_09085"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOJ67113.1"
                     /translation="MKKFNSKIVFTALAVLLTASCTNVEDVYIEKPTSDIIYSETFQN
                     GLGDFKAVNVSGDQVWSYDNKGYAIMTGFVNPTNYANEDWLISPEIDLTNITSAYFSF
                     DYVMRFSNDPVNDATVWVLADHAYSPDTLPDVSQWVKLNLKPFVDPNNWDFSNSNDIS
                     LNDFVGKKIRIAFRYISSATKAGTWEIKNFTVKKGTAKVVESNDGSEEKPFTVKEAIQ
                     NQTGVSAWVKGYIVGYIWSGTQNNYVFSADTCTQATNIILADTNDLS"
BASE COUNT          632 a          358 c          373 g          559 t
ORIGIN      
        1 acagatttcc caataaatac tattatgctt ggggctttaa cgtgttcttc aatataggat
       61 acaagtttta atgaataaaa atcatatatt tatagatatt taaaatatga cgaaaatgaa
      121 gaattttaaa ataggtggaa tttccatcct tctggcagca atttttacat cttgtgccga
      181 aacagatttt gatgcaattc ccgtcaacaa gttgatggga gattcattga ctactactta
      241 taccattgaa caattgaaga ataatttttt gactttaaac gatgcttatt ccgatacggt
      301 caattataaa ggtggcttat atacagctga tcccattcag gccgaatcgg acattgtaat
      361 cagtggttac atcacttcta ctgatgtgga tggaaatgtt tacaagtatt ttgttgttca
      421 ggaacccgga gctaacggtc aagccattaa agtttcgatt gatgcttcag gattatctgc
      481 aatttatcca ctcggacaga aagtatggat tcgatgcaac ggtttgtaca taggacgtta
      541 tggagaagcc gaacaaattg gttcacgcta tgtaaacaag gaaagattta aagttaaaaa
      601 atcaactgga gataccattt accgaataga accaggccga atgccatttt tgattgccaa
      661 cgaacacatt cacgcttacg gaatgcctga tcctacggta attaaacccg ataccatgac
      721 cattgctcaa ataaaagcat caaatcatac taaacttgtc aacaaactgg tatgcatcaa
      781 aaatgccttc ttcacgggca aaagtgaagg cgataatctt accgatagcg aactgatttt
      841 tgctccatcc accaacggag cgggttatcc gcaattgcgc gacataaatg atggtaccgg
      901 agaaatcagt atcgccacca gcgagtatgc caagtttgca aattatccgt tacctgcttc
      961 cacatatcgt ggcgatatta ccgttatcat aggttggtat cgtaattata ctaatgcagc
     1021 aggcgcatgg cagttaactc taagaaccat taacgacctt ggaaaaggct ttgaaagtta
     1081 taaagcaaaa attcagaaat aaatccgaca ttaaattacg aaatcaaaat gaagaaattc
     1141 aattcaaaaa tagtttttac ggctttggct gtgttactta cggcaagctg taccaatgtt
     1201 gaagatgttt acattgaaaa accaacttca gatataattt attccgaaac ttttcagaat
     1261 ggtcttggag attttaaagc ggtgaatgtt tccggcgatc aggtttggtc atacgacaac
     1321 aaaggttatg ccattatgac gggatttgtc aatcccacca attatgccaa cgaagactgg
     1381 cttatttctc cggaaataga tttgaccaac atcacttcag cttatttttc atttgattat
     1441 gtgatgcgct tttcaaatga tccggtaaac gatgccactg tctgggtatt ggctgatcat
     1501 gcatacagtc ccgacacctt gccagatgtt tcgcaatggg taaaactcaa tttaaaaccg
     1561 tttgtcgatc ccaacaattg ggatttcagc aattccaacg atatcagtct gaatgatttt
     1621 gtgggaaaga aaatcagaat agcattcagg tatatttctt ccgcaacaaa agccggcact
     1681 tgggaaatta aaaatttcac ggtaaaaaaa ggtactgcta aagtagttga aagcaatgat
     1741 ggcagcgaag aaaaaccttt taccgtgaaa gaagccattc aaaatcagac tggagtttca
     1801 gcatgggtaa aagggtatat tgtgggatat atttggtcag gaactcaaaa taattatgtt
     1861 ttcagtgctg atacctgtac gcaggccacc aatatcattt tggccgatac caacgatttg
     1921 tc
//
LOCUS       DAOSCH010000194         1918 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_39841_length_1918_cov_3.640365, whole genome shotgun sequence.
ACCESSION   DAOSCH010000194 DAOSCH010000000
VERSION     DAOSCH010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39841_length_1918_cov_3.640365"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /gene="cas1"
                     /locus_tag="PLZ54_09090"
     CDS             <1..833
                     /gene="cas1"
                     /locus_tag="PLZ54_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOJ67114.1"
                     /translation="EKIKQESIVTIPIEDIGVVILDHKQITITQGLIEKLLENNCALI
                     TCDSNRMPVGLMLPLAGNTTQTERFGDQINASVPLKKQLWQQTVQAKIENQAYVLDKM
                     AGAIIKNMNAWAADVKSGDPDNYEARAAVYYWANLFPSIQGFSRNRDGIPPNNLLNYG
                     YAVLRAVVARSLVASGLLPTLGIHHRNKYNAYCLADDVMEPYRPFVDRLVFEIVAENG
                     AIDEISQPIKTKLLGIPVLDVMVNGQRSPLMVAVGQTTASLYQCFAGEVRKIKYPSFT
                     "
     gene            861..1214
                     /locus_tag="PLZ54_09095"
     CDS             861..1214
                     /locus_tag="PLZ54_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOJ67115.1"
                     /translation="MKENVVKDKSFRFAVRIVRLYQFLVNETSEYILSKQLLRSGTSI
                     GANIEEADSGQTKKDFIAKLSISLKEAKETHYWLRLMFECNYFDEKMYKSFIDECEEL
                     IMLLTSIIKTSRQNL"
     gene            1300..1632
                     /gene="cas2"
                     /locus_tag="PLZ54_09100"
     CDS             1300..1632
                     /gene="cas2"
                     /locus_tag="PLZ54_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019036766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOJ67116.1"
                     /translation="MERFSEYRIMWIFVFFDLPTETKKDRKRYVDFRDKLLKDGFTMF
                     QFSIYLRHCASMENAQVHIKRVKKNLPPKGNIGILCITDKQFGNMELFYGKEEKEITT
                     PYQQLELF"
BASE COUNT          651 a          320 c          369 g          578 t
ORIGIN      
        1 ccgaaaaaat caaacaggaa tcgattgtta ccattcccat cgaagacatt ggcgtggtga
       61 ttctcgatca taaacagata accattacgc aaggtctgat agaaaaacta cttgaaaaca
      121 attgtgcgct tatcacttgc gacagcaacc gcatgcctgt tggactgatg ttgcctttag
      181 caggaaacac gactcaaacg gaacgattcg gcgatcaaat caatgcttct gttccgctaa
      241 aaaagcagct ttggcaacaa accgtgcagg ccaaaattga aaatcaggct tacgtgctcg
      301 ataaaatggc cggagcaatc attaaaaaca tgaacgcttg ggcagccgac gtcaaaagtg
      361 gcgatcccga caattacgaa gcgcgtgcag cggtgtatta ttgggcgaat ctctttccca
      421 gcattcaagg tttttcaaga aatcgggacg gcattcctcc caataatctg ttgaattatg
      481 gatatgcggt tttacgcgcg gtggtagccc gatcacttgt tgcaagtgga ttgcttccga
      541 cgctgggcat tcatcatcgc aacaaataca acgcctattg tttggctgac gatgtgatgg
      601 aaccataccg tccatttgta gatcgattgg ttttcgagat tgtagcagag aatggagcaa
      661 tagacgaaat atcccaaccc attaaaacaa aattattggg gattcctgtt ctcgatgtaa
      721 tggttaacgg gcagcgcagt ccgctgatgg tggcggtagg gcaaaccacc gcgtcgctct
      781 atcaatgttt cgcgggagag gttagaaaga taaaatatcc gagctttact taaatgtaga
      841 atgaataatg aataatggga atgaaagaga atgtggtgaa agataaaagt tttcgatttg
      901 cagtaagaat tgtcaggtta tatcaatttt tagtgaatga gacaagtgaa tatatcttat
      961 caaaacaatt gttgcggagt ggaacttcta ttggtgctaa tattgaggaa gcagattcag
     1021 gtcaaacaaa aaaagatttt attgcgaaac tatcaatttc gcttaaagaa gctaaagaaa
     1081 cgcattattg gttgagacta atgtttgaat gcaattattt cgacgaaaaa atgtacaaat
     1141 cgtttattga tgaatgtgaa gaattaatca tgttgctaac ttccattatt aaaacatcac
     1201 gacaaaatct ttaaaattat tcattcttta ttttgaactt tattctacat tctacattct
     1261 acattttaca ttttacattc tacattctac attatttaga tggaacggtt tagtgaatat
     1321 agaattatgt ggatttttgt attttttgat cttcctactg aaacaaaaaa agaccggaaa
     1381 aggtatgttg attttaggga taaactgtta aaagatggtt ttaccatgtt tcaattttca
     1441 atttatttaa ggcattgtgc cagtatggaa aatgcccaag tgcatatcaa acgagttaag
     1501 aaaaatttgc cacccaaagg gaacattggc attttatgta ttactgataa acagttcggg
     1561 aatatggagc tattttatgg aaaagaagaa aaagaaatta caacccccta tcaacaattg
     1621 gaattatttt gattattggt tttagaatag aatttagatt tgctttataa acaaaaatcc
     1681 cgcctaaaaa cgggattttc tgttttctga tcaacatttt ttattttcta atttcacctg
     1741 taaatatctg atattttgac cgtaactaac atccagttgt atcagaatat caaagatata
     1801 aaatttgaaa gcaaatcaca actgtaccgt gctaaaacat tttctttgaa agttgtatca
     1861 gaatatcaaa gatataaaat ttgaaagcaa atcacaacga attaatttaa taaacaat
//
LOCUS       DAOSCH010000195         1827 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_42757_length_1827_cov_5.886005, whole genome shotgun sequence.
ACCESSION   DAOSCH010000195 DAOSCH010000000
VERSION     DAOSCH010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42757_length_1827_cov_5.886005"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..1387)
                     /locus_tag="PLZ54_09105"
     CDS             complement(251..1387)
                     /locus_tag="PLZ54_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOJ67117.1"
                     /translation="MEKVPFTYGKIAADEDFTDRQAELKRLISNFDGLVNTILISPRR
                     WGKTSLVKQAGSICESKNNNLKVIFIDIFNIRDEREFYLSLSNRILKTTSNSWEDFTA
                     NAKLFLSRFIPTVSFSPDKQTDLSFGISWKELEKQPDDILDLAENIAQKKNMQLVICI
                     DEFQNIMHFSDPEAFQQKLRSHWQNHHNVAYCLYGSKRHMLLDVFSNSSQPFYKFGDL
                     MLLDKIPEKDWIVFLCKRFEDTGKRLSTEDARLIAQLADNHPYYVQQLAQQAWFRTKD
                     TCSQKIILSAYEDIINQLSLLFANSTEKLSSFQIHLLKAILAGEKQLTAKETLEKYQL
                     GTSANVVRLKRLLIENEILDDTSEELSFLDPMYKYWLQKNFFKE"
BASE COUNT          554 a          320 c          374 g          579 t
ORIGIN      
        1 aatcaatgtg ataatgtgtc catgaaattc aatgggaaga tattttaaaa gagacggatt
       61 gagctgcgta gcagcgataa tatttgtagc aaaagaaaga aaaaatacat ctcttgcaga
      121 tgttcgcaaa taagtatcgc agaaggtcgc agaaaaaatc agcgggaatc agcaaaagaa
      181 aagaaaaatc atctctcgca gatgttctca gataaacttt acggataatt atggaaaaac
      241 catgaaaacc ttattccttg aaaaaattct tttgaagcca atatttatac atcggatcta
      301 aaaacgataa ttcttcactt gtatcatcca gtatctcatt ttcgatcaat agtcgtttga
      361 gtcgtaccac attggcggaa gtgcccaatt ggtatttttc gagtgtttct ttcgctgtca
      421 attgtttttc tcctgctaag atggctttca gcaaatgaat ctgaaaggaa gacaattttt
      481 ccgtgctatt ggcaaacaga aggctgagtt gattgatgat atcttcgtat gcggagagta
      541 tgattttttg agagcatgtg tcctttgtcc taaaccatgc ttgttgggct aattgctgca
      601 cataataggg gtgattgtcg gcaagttgag cgattaatcg ggcatcttca gttgaaagtc
      661 ttttccccgt atcttcgaat cttttacaga ggaaaacaat ccaatccttt tcgggaattt
      721 tatccaggag catcaaatcg ccgaatttgt aaaacggttg tgaagaattg ctgaagacat
      781 ccaacagcat gtgacgtttg ctgccataaa ggcaataagc gacattatga tgattctgcc
      841 aatgcgaacg cagtttttgt tgaaaagctt cgggatcgga gaagtgcatg atattttgaa
      901 attcatcgat acatattacc agttgcatat tttttttctg tgctatgttt tctgccaaat
      961 ccagaatgtc atccggttgc ttttccaatt ccttccaact gataccgaag gaaaggtcgg
     1021 tttgcttgtc gggtgagaaa gaaacagtgg gaataaacct tgaaagaaac agtttggcat
     1081 tagcggtaaa atcctcccag ctgttagaag tggttttcaa aatgcggttg ctaagggaca
     1141 aataaaattc tcgttcgtcg cgaatattga atatatcaat aaaaattact ttcagattat
     1201 tatttttgct ttcgcagatt gagccagctt gttttaccag tgaagttttg ccccatcggc
     1261 gaggcgagat gagaatggta ttgaccaaac cgtcgaaatt actgataagt cttttcaatt
     1321 cagcttgccg atcggtgaaa tcttcatcgg ccgcaatttt tccataggtg aagggtactt
     1381 tttccatttt attgtaaagg tagcaactta ttttgtaact tacaaagttg taacttacaa
     1441 agttataact taatccgatt actggttgac ttgcattatc attcgttctg tagaggctgg
     1501 atttattctt gaaaatttgc gtctgcgtat ggaagagcag aatatcgaaa ccactccgct
     1561 atggaaactg atgcatctgt agcctgtgtt tgcggattat ccgtattatg gggacaacaa
     1621 ggcagaaaaa ttattcaacg accgacttgc ctaccttctg tccctacttt aactgatgag
     1681 gatatccaaa gaggggttga gataatcgga aagatgtgtt atttgattga atttgctatg
     1741 ccgtacctca atgttgacga ttaacatttt gttttaataa attctgacaa tttcaaaatt
     1801 aatactccca taaatcgact tcaaaat
//
LOCUS       DAOSCH010000196         1823 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_42898_length_1823_cov_7.335407, whole genome shotgun sequence.
ACCESSION   DAOSCH010000196 DAOSCH010000000
VERSION     DAOSCH010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42898_length_1823_cov_7.335407"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..376
                     /locus_tag="PLZ54_09110"
     CDS             116..376
                     /locus_tag="PLZ54_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67118.1"
                     /translation="MPTMVASKLHLMLLMKRIVVSDNLFLIIVKRTTEGNTGEIFDAS
                     FIRCQSPTRSPNQNDELMALLLSYASLSAINPVESVCKFWTH"
     gene            complement(618..>1823)
                     /locus_tag="PLZ54_09115"
     CDS             complement(618..>1823)
                     /locus_tag="PLZ54_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67119.1"
                     /translation="LILPIESLFVFGTNAKNLREIIATLKAEKQDMNLGEAFILNPEV
                     QQHLLLVPIYKNSERIFAEESTPQKYLISRKDFDITSQFYKFLGDRIALVKHGCEVKV
                     LKKAKESFAEKERYYDFGEKNSLFEPELILDRIFDYLGVKSKEFDRFKQLENEIVHFK
                     KVRFIDGDAKYKEIRQKIEAMKHYPEKKKEIDESYGNIPPEQYNKQLKLFEESCKYEV
                     NNQEIKIKYLANHYYLPVIVSETEKIDYLKHIINVDSEKRFIEQLEEYLQKDNNVFKQ
                     FDWWMFSKLDQTLDEVVIPYYNPKENNIANFKPDFIFWMQKGSRYLILFVDPKGTEHT
                     DGYRKIDGYSRIFEIGEQKQSRDFSYNGLTINTKLLFKSREIAKVLDNYKRYWFDNFD
                     DFANKISSF"
BASE COUNT          509 a          350 c          304 g          660 t
ORIGIN      
        1 caaaaacatt cacctacaaa gtcgtgttgc gctggggtga ccgccagtat tgattggtca
       61 gcgagatgcc caggttccat actttaaaaa tgttgtggtt gacagtaaaa ctgcgatgcc
      121 gacaatggtt gccagcaaac tccatttaat gttactgatg aaaagaattg ttgtttctga
      181 taatctcttc ctcattatag ttaaaagaac tactgaaggt aataccggcg aaatttttga
      241 tgcttctttt atccggtgtc agtcgccaac acgaagccca aatcaaaatg atgaattgat
      301 ggctttgctt ctttcatatg cctctctttc agccatcaac cctgtagaat ccgtttgtaa
      361 attttggaca cattgaatac aggatttcta acctttttgt ctttcctgta cctgatgata
      421 atgctttcat ccattacatt ttatcaattt tgtggaattg cgcaatgaaa tttagaaaaa
      481 acaggaggtt ttttgcaaat ctaccctggg aagtaaggga caaagcccga agtgaactgg
      541 ctaatttgtt atcgtggagt agttctggta atctacctac agttttttat gccagagaat
      601 ctggcagagt atcaatctta aaaggaactt attttgttag caaaatcatc aaaattatca
      661 aaccagtatc gtttgtaatt atccaaaact tttgctattt ctctcgattt gaacaatagt
      721 tttgtgttta tggtaagccc gttgtatgaa aaatctctgc tttgtttttg ttcgccaatt
      781 tcaaatatcc ttgaataccc gtcaattttt ctgtaaccgt ctgtgtgttc agttcctttt
      841 gggtcgacaa acaaaatcaa atatcggcta cctttttgca tccaaaagat aaagtccggc
      901 ttgaaattag cgatattgtt ttcctttgga ttataataag gaataacaac ttcatccagg
      961 gtttggtcaa gttttgagaa catccaccag tcaaattgtt tgaacacatt gttatctttt
     1021 tgtaaatact cttctaactg ctcaataaat cttttctcgc tatcaacatt aataatatgc
     1081 ttgagataat caattttttc tgtttccgaa actatcactg gaagatagta atggttagcc
     1141 aaatacttaa ttttgatttc ttggttattt acttcgtatt tacacgattc ttcaaatagt
     1201 ttgagttgtt tattatattg ttcgggcggt atgttaccat aactttcgtc tatttctttt
     1261 ttcttttcag gatagtgctt catcgcctca attttttgtc tgatttcttt gtattttgca
     1321 tcaccgtcaa taaatctaac ctttttaaag tggactattt cgttttccag ctgcttaaac
     1381 ctgtcaaact ccttgctctt cactccgaga tagtcaaaaa tccggtcaag aattaactct
     1441 ggctcaaaga gtgagttttt ttcgccaaaa tcatagtatc gctctttttc cgcaaaactt
     1501 tcttttgctt tctttaaaac cttcacttcg caaccatgtt taaccaaagc gattctatcg
     1561 cccagaaatt tgtaaaattg tgaagttata tcaaaatctt tgcggctgat aagatatttt
     1621 tgtggggtac tttcctcagc aaaaatcctt tcagagtttt tgtaaatcgg caccaaaaga
     1681 aggtgttgtt gtacttctgg atttaaaatg aacgcttcgc ccaaattcat gtcttgtttt
     1741 tctgccttca gtgttgcgat tatctctcgt aagtttttcg cattggtccc aaacacaaac
     1801 aagctctcaa tcggcaaaat cag
//
LOCUS       DAOSCH010000197         1804 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_43521_length_1804_cov_4.543739, whole genome shotgun sequence.
ACCESSION   DAOSCH010000197 DAOSCH010000000
VERSION     DAOSCH010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43521_length_1804_cov_4.543739"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..138)
                     /locus_tag="PLZ54_09120"
     tRNA            complement(65..138)
                     /locus_tag="PLZ54_09120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(102..104),aa:Arg,seq:acg)
     gene            450..1304
                     /gene="panC"
                     /locus_tag="PLZ54_09125"
     CDS             450..1304
                     /gene="panC"
                     /locus_tag="PLZ54_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010536904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOJ67120.1"
                     /translation="MKVFSTKKELQKEMEQIRKEGKTVGFVPTMGALHEGHLSLINRS
                     VAENDVTAVSIFVNPTQFNNRSDLEKYPRDLQHDLSLLEKNGCDIVFSPSVDEMYDKN
                     EFESVFEFDFGGLDQVMEGKFRPGHFNGVVQVVSKLFNLVQPTKAYFGEKDFQQLAII
                     RRMVNLMNFDIEIVGCPIVREKDGLAMSSRNLRLNAEERQKAVEISKTLFESKKLISN
                     FSPEQVQNWVIARLNSFSGLEVEYFEIVHQTTLQPIHSWSEPAIGCIAVFCGEVRLID
                     NIKYNDVQ"
     gene            1352..1705
                     /locus_tag="PLZ54_09130"
     CDS             1352..1705
                     /locus_tag="PLZ54_09130"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOJ67121.1"
                     /translation="MQIEILKSKIHRVSVTDANLNYIGSITLDQDLMDAANIIPYEKV
                     SIVNNNNGERIETYVIPGERGSGMVCLNGAAARKVQIGDVVIIMSFATMDFEEAKKFT
                     PVVIYPDTETNQLKK"
     gene            1721..>1804
                     /gene="hisH"
                     /locus_tag="PLZ54_09135"
     CDS             1721..>1804
                     /gene="hisH"
                     /locus_tag="PLZ54_09135"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004297048.1"
                     /note="with HisF IGPS catalyzes the conversion of
                     phosphoribulosyl-formimino-5-aminoimidazole-4-carboxamide
                     ribonucleotide phosphate and glutamine to
                     imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamide ribonucleotide, and
                     glutamate in histidine biosynthesis; the HisH subunit
                     provides the glutamine amidotransferase activity that
                     produces the ammonia necessary to HisF for the synthesis
                     of imidazole-glycerol phosphate and
                     5-aminoimidazol-4-carboxamide ribonucleotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOJ67122.1"
                     /translation="MKVALVKYNAGNIRSVLFALERLGIEAV"
BASE COUNT          580 a          319 c          378 g          527 t
ORIGIN      
        1 cgcaatatcc aataaaaaag agactgtctc tacttttgag acagcctctt ttttaaatta
       61 aatgtgaccc cgacaggatt caaacctgta accttctgat ccgtagtcag atgctctatt
      121 cagttgagcc acggggccat tttcgaagtg caaagataat gtatattttt aaaacgagca
      181 aagaaaattt ttaaaaatgt agatagtaga ttcaacgttc caaagtttat acaattggtc
      241 ttcggcttgc tttaggaaaa attaagaagc aatcgtgcat atccaaataa gttttcgtaa
      301 ttttgtaacc caattttgat ggtattcaaa attggaactg tcaaattttc aaaaaattag
      361 gtctttcgga actgaaaatc tttgattcga ctgctttaaa gttagcgtca aaagttctgt
      421 tcgacttgtt gaaaaaatgg tgaaaacgca tgaaagtatt ctcaaccaaa aaggagcttc
      481 aaaaggagat ggagcaaatc aggaaagaag gaaaaacagt cggatttgtt ccaaccatgg
      541 gcgctttgca cgagggtcat ttgtccctga tcaatcgtag cgtagccgaa aatgatgtta
      601 ccgcagtaag tatttttgtc aatcctactc aatttaacaa ccggtcggat ttggaaaaat
      661 accctcgcga tttgcagcac gatttgtcat tactcgaaaa aaacggctgc gatattgttt
      721 tctcgccctc tgttgacgaa atgtacgaca aaaacgaatt tgaaagtgtt tttgagtttg
      781 attttggcgg attggaccag gttatggaag gaaaattcag gccgggacat ttcaatggtg
      841 ttgtgcaggt tgtcagcaaa ttgtttaact tggtgcaacc caccaaagcc tattttggtg
      901 aaaaagattt tcaacagttg gccatcattc gtcgaatggt aaacttaatg aatttcgata
      961 tcgaaattgt tggttgtccc attgtgcgtg aaaaagatgg tttggccatg agcagccgca
     1021 atttaaggct caatgcggaa gaacgtcaaa aagccgttga aatttccaaa actttatttg
     1081 aaagtaaaaa actgatttca aacttttccc ctgaacaagt acaaaattgg gtgattgccc
     1141 gattgaattc cttttcgggc ttggaagtcg aatactttga aatcgttcat caaactacac
     1201 tgcaacccat tcattcgtgg tcagagcctg ccattggctg catagctgtt ttttgcggtg
     1261 aagtgagact gattgacaat attaaataca atgacgttca ataagttgca caactgcagc
     1321 tttatttctt taacacaaag aatttgaaaa tatgcaaata gaaattctaa aatcgaaaat
     1381 tcacagggtt tcggtaacag atgccaattt aaactatatc ggcagcatta cgctcgacca
     1441 ggatttgatg gatgcggcca acattattcc atacgaaaaa gttagtatag tcaacaacaa
     1501 taatggtgaa agaattgaga cttacgttat tcccggagaa agaggctcag gtatggtttg
     1561 cttgaatggt gcagccgccc gcaaagttca aattggcgac gtggttatta tcatgtcttt
     1621 tgccacaatg gactttgaag aagcaaaaaa gtttacaccg gttgttattt atcccgatac
     1681 ggagaccaac caattgaaaa aataaagacc ggttgagaca atgaaagttg ctttggtgaa
     1741 atacaatgcc gggaacatcc gatcggtact ttttgctttg gaacgactgg gaattgaagc
     1801 ggtt
//
LOCUS       DAOSCH010000198         1785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_44180_length_1785_cov_5.879191, whole genome shotgun sequence.
ACCESSION   DAOSCH010000198 DAOSCH010000000
VERSION     DAOSCH010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44180_length_1785_cov_5.879191"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..430)
                     /locus_tag="PLZ54_09140"
                     /pseudo
     CDS             complement(188..430)
                     /locus_tag="PLZ54_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546407.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            complement(461..1603)
                     /locus_tag="PLZ54_09145"
     CDS             complement(461..1603)
                     /locus_tag="PLZ54_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOJ67123.1"
                     /translation="MIELEKYQAGHFEKNLGYKYFVPSTINDEWTWKTPALNLLLEKA
                     AIKLGELNSYARLVPNIDLFIQLHVTKEAVVSSHIEGTKTNMDEALLPVEEIHPERRD
                     DWQEVNNYIKALNNAIEELKSLPISSRLLKETHKILLQSVRGEHKLPGEFRTSQNWIG
                     GTSLHDAVFIPPSHHLVNELMSDLEKFLNNDEINVPLLIRIAIAHYQFETIHPFLDGN
                     GRIGRLMITLFLVSEGILDKPLLYPSLYFEKNKSLYYDNLSNVRTKNDMLQWIKYFLV
                     GIEQSAAKAVETLSNILKLKESIEKEINSNFGRRSNSAHTLVHFLFKDPVISIDKAAK
                     ECNLSFKAANDLVALMQKFNYLKELTGQSRNRIFIFEPYLKIFDND"
BASE COUNT          513 a          341 c          294 g          637 t
ORIGIN      
        1 tgcttcccgt ataatagctc ccgtcgctgc attccaatat gtacatgtac ccttttttca
       61 ttcggtaagt tttatctcaa aaaaaaatgg atttattgtt tgttgtcgct tttgggcaat
      121 ttgggacttg ccttttcaat ttgtttttct tcggcagcca cacgacgttc aagttttttg
      181 atgttttccc aatttcaacc attttcgtga cctcacgaaa atgatcatcg acattgatat
      241 tctgtatttt acatgagtca acagctctgc cgattgcgac cttaaaattc tcccaacggg
      301 catatcccaa tgcgaccatc aattcccggg caaaccaaac tcccgcttgt tctctatcgt
      361 catcacttgt aataaagtga ctaatttcat cgaaaactga ttgtaattgt gaaatgcgat
      421 tttgatccat atttatagaa tgatttgata tatccggtcg tcagtcattg tcaaaaatct
      481 ttaagtaggg ctcaaaaata aaaatacggt tgcggctttg cccggttaat tccttaaggt
      541 aattaaattt ttgcataagt gctactaaat cattagctgc tttgaaactc agattacact
      601 ctttcgctgc cttatcaata gagataaccg gatccttaaa tagaaaatga acaagcgtat
      661 gagcagaatt gcttcgccga ccgaaattgc tatttatctc tttttcaatg ctttctttaa
      721 gttttaatat attagaaagt gtttctaccg cttttgcagc actttgttca atgcctacta
      781 aaaaatattt tatccattgc agcatatcgt ttttggttct tacattactt aaattatcgt
      841 aatacaggct tttatttttt tcgaaataca atgatggata gagtaaaggt ttatctaaaa
      901 taccttctga aacaagaaat aaagttatca taagacgtcc gatacggcca ttcccgtcta
      961 aaaacggatg aatagtttcg aattgataat gtgcaatggc aatacgtatt agcagaggaa
     1021 cgttaatttc atcattatta agaaactttt ccaaatcgct cataagttca ttcaccaagt
     1081 ggtgagatgg cggaataaat actgcatcat gcagtgaagt acctccaatc cagttttggc
     1141 tagttctgaa ttctccgggt aatttatgtt caccccttac gctttgcagc agtattttat
     1201 gcgtttcttt tagtaagcga gaagaaatgg gcagtgattt tagttcttca atagcattat
     1261 ttaatgcctt gatgtaatta tttacttcct gccagtcgtc tctacgttcg ggatgaattt
     1321 cttcaaccgg caaaagggct tcatccatat tggttttggt tccttcaata tggcttgaaa
     1381 caacagcttc tttggttaca tgcaattgaa taaacaaatc aatattggga acgagtcgtg
     1441 cataagaatt taattcaccc aacttaatag ctgctttttc caataacaga ttcaaggccg
     1501 gcgttttcca agtccattcg tcgttaattg tactaggaac aaaatatttg taccctaaat
     1561 ttttctcaaa atgtccggct tgatattttt caagttcaat catattttta tcgataagga
     1621 aaatataaat atcgttattt tacttttagc gataaaagta aaatatagct gcctttattt
     1681 tacttttcat ccatcaattt gatttttgat aaattttcca gaatgatttc gttcaatcgt
     1741 gcttcttctt tcagctgctc ttccagttcg gctttcaggg cggtg
//
LOCUS       DAOSCH010000199         1784 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_44214_length_1784_cov_3.502024, whole genome shotgun sequence.
ACCESSION   DAOSCH010000199 DAOSCH010000000
VERSION     DAOSCH010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44214_length_1784_cov_3.502024"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="PLZ54_09150"
     CDS             <1..1009
                     /locus_tag="PLZ54_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007210802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HOJ67124.1"
                     /translation="EGTEMYNYSPFIYAYHVNNIALTGEGTINGNGSKKFATWSKFQQ
                     RGQEMLRKMGREQIPVWKRVFGEGYHFRPGFFEPFGCTNVRIEGITILDSPFWVIHPI
                     FCTNVIVRKITVRSFNSNNDGCDPESCTNVLIEDCDFNTGDDGIAIKSGRDADGWRVG
                     QPTENVVIRNCTFNSKSNGICIGSEMSGGVRNVFVENITLTKSANAIYFKSNLDRGAY
                     IENVYVRNVKADTVRTAFIRFEPNYKGERSSFHPTLFRNFVIENAFCKLSKKVGIYLA
                     GFEGYPLQNITLRNVTIDSTPVPYYLQNAENIVFDHVTVNGKQLDANPAPAEIITLKV
                     L"
     gene            1247..>1784
                     /locus_tag="PLZ54_09155"
     CDS             1247..>1784
                     /locus_tag="PLZ54_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ67125.1"
                     /translation="MTLPEWYAFSIISRKEKLQELSNIIVADAYFSKITFIQPLIKEG
                     FHVISKFRDDANLRYLYTGEYSGSGKPAHFAGKVKLKDLDSEVFTQVDINENTQAFYA
                     VVNSKSLKRDILLVVERKKNEKEKVTQRLIFSTDTQMDALEVLACYHCRFQMEFNFRD
                     AKQATGLNHAQARDLDKLY"
BASE COUNT          569 a          339 c          350 g          526 t
ORIGIN      
        1 ggaaggaacc gaaatgtaca attattcacc ttttatatat gcttaccatg ttaataatat
       61 agcattaact ggtgaaggaa ccatcaacgg gaacggaagc aaaaaatttg ctacttggag
      121 taaatttcaa cagagaggac aggaaatgtt gcgtaaaatg ggacgcgaac aaattccggt
      181 ttggaaaaga gtttttggtg aagggtatca ttttcgccct ggtttttttg aaccgtttgg
      241 atgcaccaat gttcgcatcg aaggaatcac cattcttgat tctccgtttt gggtaattca
      301 tcctattttt tgtaccaacg ttattgtgcg aaaaatcacg gttagaagtt tcaattccaa
      361 caacgacggt tgtgatccgg aatcgtgcac aaatgtattg atcgaagact gtgatttcaa
      421 taccggcgac gatggtatcg ctatcaaatc aggtcgcgat gctgatggat ggcgcgtggg
      481 acaacccact gaaaatgtgg ttatccgcaa ctgtacattt aactccaaaa gtaacggtat
      541 atgtattgga agtgaaatgt ccggaggagt tcgtaatgtt tttgttgaaa acattacatt
      601 aacaaaatct gctaatgcta tttatttcaa gtcgaacctc gaccggggag cttatattga
      661 aaacgtttat gttcgcaacg taaaagccga caccgtacgt actgccttta ttcgttttga
      721 acccaattat aaaggagaac gctcttcgtt ccatccaacg ttgttccgca attttgttat
      781 cgaaaatgca ttctgtaaat tatcgaaaaa ggtaggtatt tatcttgcag gctttgaagg
      841 ctatcctctt caaaacatca ctttgcgtaa tgttacgata gactcaacgc cagttcctta
      901 ttatcttcag aatgccgaaa atattgtttt cgatcatgta accgtaaatg gaaagcaatt
      961 ggatgcaaat ccagctccgg cagaaataat aacacttaaa gtgctgtaaa taaattattt
     1021 atacaataca tttttctttt ctgccagtca ttcttttgac ccgagtcata ttactaaatc
     1081 aggaaaaaag acaccctatc ttggttcgtt ttggtcaggt tctgaccaat gtatcaaaaa
     1141 aggtcttgaa atagctgaat tttctctgct ggacattgat tcaaatcaga gcttccattt
     1201 agaagcggca caaacaccac cggtagaaac gctaaagaca gtttccatga cattgcccga
     1261 atggtatgca ttttcaatca ttagccgaaa agaaaaactg caggaacttt ccaatataat
     1321 tgttgccgat gcatactttt ccaagataac attcattcaa ccactcataa aggaaggatt
     1381 tcatgttata tccaaattca gagacgatgc caaccttcgg tatctctaca ccggtgaata
     1441 ctccggaagc ggaaagcctg ctcattttgc gggcaaagtc aaactcaaag atttggacag
     1501 cgaagttttt acccaagtcg atatcaatga gaatactcaa gcattttatg ctgtagtgaa
     1561 ttcaaagtca ttaaaaagag atatcctgct ggttgtcgag agaaaaaaga atgaaaaaga
     1621 aaaagtaact caacgactga ttttctcaac cgatacacaa atggatgctc ttgaagtttt
     1681 agcctgttat cattgtcgct ttcaaatgga gttcaatttc agagatgcta agcaggctac
     1741 cggactgaat catgctcaag caagagattt ggataaactt tatt
//
LOCUS       DAOSCH010000200         1758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_45144_length_1758_cov_18.634175, whole genome shotgun
            sequence.
ACCESSION   DAOSCH010000200 DAOSCH010000000
VERSION     DAOSCH010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45144_length_1758_cov_18.634175"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(521..>1758)
                     /locus_tag="PLZ54_09160"
     CDS             complement(521..>1758)
                     /locus_tag="PLZ54_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOJ67126.1"
                     /translation="PIETRFFLKQTNAAGQGVLKKIAALEYWMNGNSANRTQRTLNGQ
                     TSFTWEELIDVAGIPDGANTFNARFKDEYGVWSPIETRFFVKQSAISGEGNKKLTTLE
                     YWMDANSTNRISKTFTPSNPYLWEELIPTDDVPNGLHTFNVRWKDDKGIWTMVHTQFF
                     FKDEKKFAQIGENKIVGYRLWFSEEPDYFENIPVIAMENVVAINDSVPLTYLPKGRHQ
                     IAYQLKDARGVWSPVITDSIDKPDHALFSFVADKLEIIQGETVKFTPSTKLFIDSIVW
                     SFGDGMTEVNFEPEHKYDSIGKFDVMATVWHKGTNEGIPYEEIKYITVTSTGLLSPEV
                     YSLKMYPIPAKNYLTIECPSLPMKSVQIISLNGKVMKEFLTENSEKAVLPIGNLQAGT
                     YIIVVKTDKGWLSGKIVKE"
BASE COUNT          500 a          322 c          349 g          587 t
ORIGIN      
        1 ttgacctctc cggataaatt tttaaagtac ggagaaaata ccggattgac agcttctgcc
       61 gagaaacaaa acaaaaaaga aaaaataaca aataggcaaa gtgcttgttt tttgaagagg
      121 tgtaaaaaat ttttcatggt agtgattttt tgaaaatgat ttttatctaa attttcttag
      181 tacatgacaa aaattgagac atttgtttta tatatgtgta aaaacaggat atataagtca
      241 agctcttggg tttgaccttg tccggtgagc cgaaaaacaa gtgagaaaag cgtttaaaaa
      301 tcgtccttgt ttgagttccg acgtatttcg tcggaatgag tttgggcgat ttcagctttt
      361 cgagcgtaag tttttcgagt gaagtggaca cagtcttgaa ttttttttgc ttactttttt
      421 gtttcaagac aaaaaagtaa gtggggtttg gggcaaagcc ccgttattaa atatctgaaa
      481 attttttcta caattttttt gtcatgtact aaactaaatt ttattctttg actatttttc
      541 cggacagcca acctttatcg gtttttacta cgataatgta agttcccgct tgtagattcc
      601 caataggcaa tactgctttt tcagaatttt cagtcaaaaa ctctttcatt acttttccgt
      661 tcaatgaaat gatttggacg gatttcattg gcaaagaagg acattcgatt gtcagataat
      721 ttttagcagg aattggatac atttttaatg aataaacttc aggagaaagc aaaccggtag
      781 aagtaaccgt aatgtatttg atttcttcgt aaggaatgcc ttcgtttgtt cccttatgcc
      841 aaactgtagc cataacatcg aattttccaa tcgaatcgta tttgtgttcc ggttcaaaat
      901 ttacttcggt cattccatcg ccaaaactcc agacaatgct gtcgataaaa agcttggtcg
      961 atggagtaaa tttgacggtt tctccctgta tgatttcgag tttatcggca acaaaactga
     1021 aaagtgcatg atccggcttg tcgatactgt cggttatcac cggcgaccat acgccgcgag
     1081 catctttgag ttgataggca atttgatgcc ttccttttgg taaataggta agtggtaccg
     1141 aatcgtttat tgcaacaaca ttctccattg ctattacagg aatattttca aaataatcgg
     1201 gttcttccga aaaccaaagc cggtagccga caattttatt ttctccaatt tgggcaaatt
     1261 ttttctcatc tttaaagaag aactgagtat gcaccatcgt ccatattcct ttgtcgtctt
     1321 tccagcgaac gttgaaagta tgcaaaccgt tgggtacgtc atctgtagga ataagctctt
     1381 cccatagata tggattactt ggagtgaatg ttttgcttat tctgtttgtc gagtttgcgt
     1441 ccatccaata ttcaagagtt gttaatttct tgtttccttc acccgaaatt gctgattgtt
     1501 tgacaaaaaa tcgggtttca atgggcgacc aaacgccgta ctcgtccttg aaacgggcat
     1561 tgaacgtgtt ggcaccatcg ggaatcccgg caacatcaat caactcttcc caagtgaaag
     1621 aggtttgccc gttcaaggtt cgctgtgttc tgttggcaga atttccattc atccaatatt
     1681 caagagcggc aatttttttc aatacgccct gtccggcagc attggtttgc ttgagaaaaa
     1741 atcgggtttc aatgggcg
//
LOCUS       DAOSCH010000201         1705 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_47198_length_1705_cov_4.556970, whole genome shotgun sequence.
ACCESSION   DAOSCH010000201 DAOSCH010000000
VERSION     DAOSCH010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47198_length_1705_cov_4.556970"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..>1705
                     /locus_tag="PLZ54_09165"
     CDS             47..>1705
                     /locus_tag="PLZ54_09165"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HOJ67127.1"
                     /translation="MKKNNKTAKKIFSSLIFIGLSALISASGQHPNLLLTKQGVKEIQ
                     ASLGKYANFDKSVNELKTIADKALNSEIIVPLPLNEGGGYTHEKHKNNYYEMNAAGTM
                     YQISGKKEYAEFVRKMLMKYAELYPTLGLHPSKRSETPGKIFWQLLNDCVWLFHTAFA
                     YDCIYDYLNASEKTNLEKNLFRPMAEFISNGSPANNEVFNKMHNHGTWATASVGMIGY
                     VMGDKNLVEKALYGSKKDNKSGFIRQLDVLFSPDGYYTEGPYYQRYAIWPFMVFAQVI
                     ENNQPELKIFSYRDSVLLKATNTLIQCAYNGNIFYLNDALKKNYKTQEIVYAVDIAYK
                     NNPKNTFLLDIAQQQKSFIISDAGIATAKSLNIVKYEPFQFRSVLLRDGANGDEGALG
                     IIRAGKKDKTQMCLTFKATSHGLSHGHYDKLSISMYDGGNFVLTDYGAVRFLNIEPKS
                     GGNYAKENHSWAAQTIAHNTVTVDETSNFNGNIKVSSLHHSDIQYYDFSSNQIQIISG
                     CEDNAYKNVKMQRTVAVIENKTFSYPIVIDIFRIISDSTHTLDFP"
BASE COUNT          594 a          304 c          307 g          500 t
ORIGIN      
        1 agacatgtgg gttccatcag acctttaaaa aataaattat attctgatga aaaaaaacaa
       61 caaaacagct aaaaaaattt tttcttcatt gatattcatt ggtctatctg ctttaatatc
      121 agcttcgggt caacatccga atctgctgct gactaagcag ggagtaaaag aaatacaagc
      181 atcactggga aaatatgcca actttgacaa gtcggtaaac gaattaaaaa cgatcgccga
      241 taaagcattg aattcggaaa ttattgttcc gttgccgttg aatgaaggtg gtggatatac
      301 acacgaaaag cacaagaata attactatga aatgaacgca gccggaacga tgtaccaaat
      361 ttcgggtaaa aaggaatatg ctgaatttgt tcggaaaatg ctgatgaaat atgcagaatt
      421 ataccctact ttaggattgc atccatccaa aagatcagaa acaccgggta aaatattctg
      481 gcaattgctg aacgattgtg tctggctatt tcatactgca ttcgcttatg attgcattta
      541 cgattatcta aatgcctctg aaaagacaaa ccttgaaaaa aatctctttc gccctatggc
      601 agaatttatc tcgaacggga gtccggccaa caatgaggtt ttcaacaaaa tgcacaacca
      661 cggcacatgg gctacagctt cggtaggtat gatcggatat gttatgggtg ataaaaatct
      721 ggttgaaaaa gccctttatg gttctaaaaa agacaataaa tcaggattta tacgtcagtt
      781 ggatgtatta ttctctccag atggctatta tacagaagga ccttattatc aacgttatgc
      841 tatttggcca tttatggttt ttgctcaagt gattgaaaac aatcagcccg aattgaaaat
      901 tttcagttat agagacagtg ttttgttaaa agcaaccaat actttgattc aatgtgctta
      961 taatggaaat attttctatt tgaacgatgc tttaaaaaag aattataaaa cacaagaaat
     1021 agtgtacgca gttgatattg cctataaaaa taatccaaaa aatacttttc tacttgatat
     1081 tgcacaacag caaaaatcat tcatcatatc ggatgcgggc attgctacag caaagtcgct
     1141 gaatattgtg aaatatgaac ctttccagtt tcgctcagtt cttttgcgag atggagctaa
     1201 tggcgatgaa ggagcattag gcattatcag agcagggaaa aaagataaaa cacaaatgtg
     1261 tcttaccttc aaagcaactt ctcatggatt atcacatggt cattacgata aactgtccat
     1321 ttctatgtat gatggaggaa atttcgtttt aaccgattat ggcgctgtcc gctttctcaa
     1381 tatagaaccg aaaagcggtg gaaattatgc aaaagaaaat cattcctggg ctgcacaaac
     1441 cattgcccat aataccgtaa cagttgatga aacaagtaat tttaatggca atattaaagt
     1501 ttcctccctt catcattccg atattcaata ttatgacttt tcaagtaatc aaatacaaat
     1561 catttccggc tgcgaagaca atgcctataa aaatgtgaaa atgcagcgaa ctgtagctgt
     1621 aatcgagaac aaaacatttt cgtatccaat tgtcatagat atttttagaa tcatctccga
     1681 ctctacacat acacttgatt tccct
//
LOCUS       DAOSCH010000202         1683 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_48136_length_1683_cov_5.697789, whole genome shotgun sequence.
ACCESSION   DAOSCH010000202 DAOSCH010000000
VERSION     DAOSCH010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48136_length_1683_cov_5.697789"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PLZ54_09170"
     CDS             <1..618
                     /locus_tag="PLZ54_09170"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HOJ67128.1"
                     /translation="DGMIITGAPVEQLEYEEVTYWPEVQEIFDWSRKHVTSTMFICWA
                     AQAGLYHFYGIPKYPLEKKMFGVFEHYIHNPQNPIFRGFDDIFYVPHSRYTEVRAEDI
                     KKNPELILLSESDESGVYMVMGRNGREFFITGHSEYSATTLDNEYKRDLAKGLPIDMP
                     KNYYRDNNPANEPLVRWRSHANLLFTNWLNYFVYQETPYDLSKIH"
BASE COUNT          501 a          310 c          271 g          601 t
ORIGIN      
        1 gatgggatga ttattacggg ggctccggta gaacagctcg aatacgaaga agttacttat
       61 tggcctgaag ttcaagagat atttgactgg tcgcgcaagc atgtaacttc aaccatgttt
      121 atttgctggg cagcccaagc cggattgtat catttttatg gtattccaaa atatccgttg
      181 gaaaagaaaa tgtttggcgt tttcgagcat tatattcata atccgcaaaa tcccattttt
      241 cgcgggttcg acgacatttt ttatgtgccc cacagccgtt ataccgaagt cagggcggag
      301 gatatcaaaa aaaatcctga actgatattg ctttccgaat cggacgaatc gggtgtttat
      361 atggtaatgg gacgcaacgg aagagagttt ttcattacgg gacattcgga atactcagct
      421 accacactgg ataatgaata caaaagagat ttggcaaaag gtttgcctat tgatatgcca
      481 aaaaactact atcgcgacaa taatcctgcc aatgaaccgc tggttcgatg gagaagtcat
      541 gccaatcttt tatttactaa ctggttgaat tatttcgttt atcaggaaac accctacgac
      601 ctttcaaaaa tccattaaag aaatatttgg tgtttaattc agtataagac aagatttttt
      661 aaaaaaataa ttgataatta actaattgta ttttttattt gtttcaattt tgaatcccct
      721 cttaaaacct gattttttca aaagggagaa aaaatcaggt ttttttgaaa agaggcatta
      781 aattttttgt tgttttgata ttttacatca cattttttgt ctcagaatag aaaaaatgca
      841 attgaagatt ttgtttcagt ttttttaaat gtcaactcta ttgtccaacg ataatggtat
      901 ttcaaagttt cttcttcagc tcttatttct caattattgg ttaaacttgt atttttgccc
      961 tttctcatct ttattataac caaacgcaaa caaagtgttt ttttctttct ttgttttcct
     1021 tataatccaa gtgtatatgc cctaccctgt aaacaccaaa tgcttctttt gaacactttt
     1081 tcttaaacaa aacctcttgt acttgtttct tgacatggtc tttcagacga caaacaaaat
     1141 ttacttcttt ctctctccag gcagcaaatt ggagatagta attataggct ttatcgtaaa
     1201 ctaatgtact tcccatcaag gatgcaaaaa tgtcaaaaac ttcttgtcgt gcctctttgc
     1261 gttactaatt ttcactaaaa aaggcgtctg actatgtcat cggtcagcat gtgtactttc
     1321 aaccctcctt tccttttccc ttcacctttg ggatttccgc ctatccgttt cattatttcg
     1381 ggaaaagtgt gatagtagta gaatcgaatg cttaaaattt ctcaaaaact gactttttct
     1441 tttcggctga cctacaaaat ttgtcgttgt atctttaacc aggactgtta atttgagttt
     1501 gaaatacaaa ttttctaccc cagacgaggc ttccaacttg tcccttccat tttacttaat
     1561 tttttttgta ggtcaacagt gattcagaac aattacagaa aaatttctct gataaactgt
     1621 ttaccataca gcaatctcat ctatgaaaat agaattattt ttttcatttt caagattttc
     1681 aat
//
LOCUS       DAOSCH010000203         1661 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_49001_length_1661_cov_5.490660, whole genome shotgun sequence.
ACCESSION   DAOSCH010000203 DAOSCH010000000
VERSION     DAOSCH010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1661
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49001_length_1661_cov_5.490660"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..677
                     /locus_tag="PLZ54_09175"
     CDS             141..677
                     /locus_tag="PLZ54_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67129.1"
                     /translation="MNNFALEIWYDEGRICTFYTVRWVTEDDDTASETDKFFNTYAVP
                     GHPLEEKAMQLFRLITECIGNRYGATDDFFDRIVKKAQELPPKPKQWVEEIKDLGIHF
                     PLRLFCYRVTENIVILFNGGIKESQSTQESKSLSMKFYEAQTFVKKIAEALQSKMIEI
                     SDDGRYLLNFDGTADIIL"
     gene            682..1089
                     /locus_tag="PLZ54_09180"
     CDS             682..1089
                     /locus_tag="PLZ54_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOJ67130.1"
                     /translation="MRSKTVDRLLQSTPKDVEIFVDWYADLVVRINQLLREKNLSKKE
                     LAERMDKRPSEISKWLSGEHNFTLRSLAKLSAELGEPLLEVPNRKPNTPFIRGYSRSV
                     HTFVVYKKIEPSKPSKVIEWQQANELNLLSNVG"
     gene            1079..1543
                     /locus_tag="PLZ54_09185"
     CDS             1079..1543
                     /locus_tag="PLZ54_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67131.1"
                     /translation="MSDSNNKFDPEKISIVEFKIVKGQVDVPEDFEIANVEAHHIDNS
                     LQLGFNLEEKLVKADFTIDIKTKSKGQNTQEARGSFHLVFIYHVENLEELAKLDANNL
                     IELNYTLANALSSVTYSTSRGILLTRLQGTALQNFILPIINPNKLLHNKKNE"
BASE COUNT          624 a          270 c          330 g          437 t
ORIGIN      
        1 tccgagaggc gcgggatatt ttgttaccta aactaatgaa cggagaaata gaagtataaa
       61 tgattgatat ataggcaaaa atgttgattt tataaaattt aacttaaaag tgaaacaaaa
      121 tttgcataat tcagttaaaa gtgaataact ttgcactcga aatatggtat gatgaaggaa
      181 gaatttgcac attctacacc gtcaggtggg ttactgaaga tgacgatacg gcatccgaaa
      241 cggataaatt ttttaatacc tatgctgtgc ctggacatcc acttgaagaa aaggcaatgc
      301 aattgtttcg attgataacc gaatgcattg gaaacagata cggagcaacc gatgattttt
      361 ttgacagaat agtaaaaaaa gcacaagaat tgccaccaaa accaaaacaa tgggtagaag
      421 aaataaaaga cttaggtatc cattttccac tgaggttatt ttgttatcgg gtaaccgaaa
      481 acattgttat acttttcaac ggtgggatta aggaatcgca atcaacacaa gaaagcaaaa
      541 gtttaagtat gaagttttat gaagcacaaa cctttgtcaa gaaaattgcg gaggcattgc
      601 aatcaaaaat gattgaaata tctgatgacg gaaggtattt gctaaatttt gacggaacag
      661 ctgacataat actataagac aatgagaagc aaaaccgtag atcgattgct gcaaagcaca
      721 cccaaagatg tagaaatctt cgtggattgg tatgccgacc tggtggttcg gataaatcag
      781 ctattgcgtg aaaaaaatct cagcaaaaaa gaattggctg aaaggatgga caaaagacca
      841 tcagaaattt ccaaatggct aagcggtgag cataatttca ctctgcgttc actagcaaaa
      901 ttgtcggcag aattgggtga accgctatta gaagtgccga acagaaaacc aaatacacct
      961 tttataagag gctacagtcg tagtgtgcac acttttgtag tttacaaaaa aattgaacct
     1021 tccaaaccat caaaagtgat tgaatggcaa caggcaaacg aattaaattt attaagcaat
     1081 gtcggatagt aacaataaat ttgaccctga aaaaatcagc attgttgagt ttaaaatagt
     1141 aaaagggcag gtggatgtgc ccgaagattt tgaaatagcg aatgtggaag cgcatcatat
     1201 agacaattcc ctacaattag gttttaactt ggaagaaaaa ctcgtaaagg cagactttac
     1261 cattgacatt aaaacaaaaa gcaaggggca aaatacccaa gaggcacgtg gaagttttca
     1321 cttagtattc atatatcatg ttgaaaattt agaagagttg gcaaaacttg acgccaataa
     1381 tttaattgaa ttgaattaca ctttagcgaa tgcactttcc tctgttacct attcaacatc
     1441 aagaggaatt cttttaaccc gtttacaagg cacagccctg caaaacttta tattgcccat
     1501 tattaacccg aacaaactgc ttcacaataa gaaaaatgaa tgacctggga atattcagaa
     1561 gataatttaa tagagcaaac agccattgat ttatttttca ataggctggg ttgggatacc
     1621 gtattagcct acaataaaga aaattttggc gaaggcagca c
//
LOCUS       DAOSCH010000204         1633 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_50235_length_1633_cov_9.425856, whole genome shotgun sequence.
ACCESSION   DAOSCH010000204 DAOSCH010000000
VERSION     DAOSCH010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50235_length_1633_cov_9.425856"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            305..646
                     /locus_tag="PLZ54_09190"
     CDS             305..646
                     /locus_tag="PLZ54_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOJ67132.1"
                     /translation="MAYPKNKTYDFDIEELARFAKAMGHPARISILRYLASMDSCFFG
                     DICQELPIAKATVSQHLTELKNAGLIRGEIEPPKVRYCIDQENWEKANEAFRQFFSNI
                     QPKDICCNPEN"
     gene            657..1076
                     /locus_tag="PLZ54_09195"
     CDS             657..1076
                     /locus_tag="PLZ54_09195"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HOJ67133.1"
                     /translation="MKILILCTGNSCRSQMAEAFLRSFDNRLTVCSAGTEPAEKVSEK
                     AIQVMREAGIDISRNIPKSVSQYLSETWDYVITVCADADRNCPVFSGKVKHRLHIGFD
                     DPAKVTGSEEFVLNEFRRIRDAIKEKFHQFYQENLQL"
     gene            1073..1429
                     /locus_tag="PLZ54_09200"
     CDS             1073..1429
                     /locus_tag="PLZ54_09200"
                     /inference="COORDINATES: protein motif:HMM:NF006749.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HOJ67134.1"
                     /translation="MNVIYTIVKVLLSSVVLVAVSEISKRSTFVGSLLASIPLVSLLA
                     IVWLYIDTKDTTQIASLSTGIFWLVIPSLVFFIVLPMLLKRSLNFWLSLVISSVLTVI
                     AYFIMLYLLKKSGIKL"
     gene            1441..>1633
                     /locus_tag="PLZ54_09205"
     CDS             1441..>1633
                     /locus_tag="PLZ54_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67135.1"
                     /translation="MEKNIKSVVKDTYTVIAQQSKEQNETSCCGVGGCCSTVDYAVFS
                     ENYRQLKGYHEEADLGLGCG"
BASE COUNT          477 a          333 c          343 g          480 t
ORIGIN      
        1 ggcgttgatc aaactacaat agaagaagta gaattactaa aagaattgat tttcaggatt
       61 ttacattaga cattaatttt atttttacag ccttttattt acaattagtt gttttttatt
      121 ttttgctaaa gatgagaaaa tgtgaggaag ccataagttc tacctttcag gcaatatctt
      181 tgatgtgaga atgtgataat aaacaaattc ctacagattt ttagaaaaaa atcttcattg
      241 tttattgtga tttgaatatt attcgtaatt ttgcgaacaa caaattcaaa tcatccatac
      301 accgatggct taccctaaaa ataaaaccta cgacttcgac atcgaagagt tggcgcgttt
      361 tgccaaagca atgggacatc ctgcccgcat cagcattctt cggtacttgg cttcgatgga
      421 cagttgtttc ttcggcgata tttgtcagga actgcccatt gcaaaggcta ccgtttcgca
      481 gcacctgaca gaattgaaaa acgccggatt aatcagaggc gaaatcgagc cgcccaaagt
      541 gagatattgc atcgatcagg agaattggga aaaagccaat gaagcttttc ggcaattctt
      601 ctccaacatt caacccaaag acatttgttg caatcccgaa aattaatttc acaggtatga
      661 aaatacttat cctttgcacc ggcaacagtt gtcgcagcca aatggcagaa gcatttctac
      721 gttcgtttga taatcgtttg acggtttgct ctgccggcac cgagccggcg gagaaagtga
      781 gcgagaaggc cattcaggta atgcgtgaag ccggtatcga tatcagccgg aacattccga
      841 aatcggtcag tcagtatctg tcggaaacat gggattatgt gattaccgtt tgcgccgatg
      901 ccgaccgcaa ttgtccggta ttttcgggaa aagtaaaaca ccggctgcat attggttttg
      961 atgatccggc caaagttaca ggttctgaag aatttgtctt aaacgagttc agaagaataa
     1021 gggacgcaat aaaagaaaaa ttccaccaat tctatcaaga aaatttacag ctatgaatgt
     1081 catttatacc atcgttaaag tactgctttc gtcggttgta ttggtagccg tttccgaaat
     1141 atccaaacgg agcacttttg tgggcagcct gttggcttcc ataccgctgg tttcgctgct
     1201 ggccatcgtt tggctgtaca tcgacaccaa agacaccaca caaattgcct cgttgtccac
     1261 cggcattttc tggcttgtga ttccttcgct tgtctttttt attgtgctgc ctatgttgct
     1321 caaaaggagt ctcaactttt ggctgtcgct ggttatctct tctgtgctga cggtcattgc
     1381 ctatttcatt atgctttatt tgctcaaaaa atcaggaata aaactctaac attcaaatct
     1441 atggaaaaaa atatcaaatc cgttgttaaa gacacttaca cggtgattgc acaacaatcg
     1501 aaagaacaaa acgaaacttc ctgctgcggt gttggcggat gttgttcgac ggtcgattac
     1561 gccgtattca gtgaaaacta ccgccaactg aaaggttatc acgaagaagc cgatttggga
     1621 ttgggatgcg gca
//
LOCUS       DAOSCH010000205         1616 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_51034_length_1616_cov_5.465727, whole genome shotgun sequence.
ACCESSION   DAOSCH010000205 DAOSCH010000000
VERSION     DAOSCH010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51034_length_1616_cov_5.465727"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLZ54_09210"
     CDS             <1..296
                     /locus_tag="PLZ54_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67136.1"
                     /translation="SCSLMKADPAKKVNVTYQDGKLKVEALHKVRNASNHFIDQVVVK
                     VNGKEMKTIRLDKQTSTASQLVEIELPDLKKGDQIEVVTRCNEFGKKNGKLIL"
     gene            complement(406..1308)
                     /locus_tag="PLZ54_09215"
     CDS             complement(406..1308)
                     /locus_tag="PLZ54_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015531658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOJ67137.1"
                     /translation="MMPPFLKSGDMVQIVSPASAIHPSYVEGAKRTLSEWNLKVLEGK
                     YARSAYGRFAGTKEERIFDLQQALDDPEVKAIFMSRGGYGLVQIIDKINFEQFKKSPK
                     WLIGFSDISILHAAVNRMGIASIHGIMAKHLAELPRTSDAVFHLREILFGRLPVYQLP
                     FHPLNRQGKITAKLLGGNLSVLMGLRGTSFDLPFEDAVLFVEDTGEKPYQVDRMIQNL
                     RIGGIFEKISGLIVGQFTDYESDPLMMQTIEEMFAEAVKNYSYPVCFNFPAGHIDYNL
                     PLLLGENITLEVSAENVLVDFNRQ"
     gene            complement(1386..>1616)
                     /locus_tag="PLZ54_09220"
     CDS             complement(1386..>1616)
                     /locus_tag="PLZ54_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOJ67138.1"
                     /translation="DSSLTFWCSLIPFTSPVVMMVRLPYGVAVWEIVLSLAILILSFI
                     ATTWVAGKIYRTGILMYGKKTSWKELWKWLNY"
BASE COUNT          540 a          332 c          301 g          443 t
ORIGIN      
        1 tttcctgttc attgatgaag gccgatccgg caaaaaaagt caatgtaact tatcaagatg
       61 gaaagctaaa agtagaagcg ttgcataaag ttagaaatgc cagcaatcat tttattgatc
      121 aggtggttgt gaaagtaaac ggaaaagaaa tgaaaacgat ccggttggac aaacaaacct
      181 caacagcatc tcaattagta gaaatcgaac ttcctgacct aaaaaaaggg gatcaaattg
      241 aagttgtaac ccgctgcaac gaatttggta aaaaaaacgg taaacttatt ttatgacatt
      301 ttgtatgtgt tttggtgtta aatgggcact caacaaattt gaatagattc tgttcaacaa
      361 tttgttgagt gttgctttat ccgattctac cgaaacacaa ggtttttatt gtctattgaa
      421 atcgaccagc acattttctg cagacacttc caaagttatg ttttcaccca ataaaagcgg
      481 taaattgtaa tcgatgtggc cggcaggaaa attaaaacaa accggataag aatagttttt
      541 tacagcttcg gcaaacattt cttcgatggt ttgcatcatc aatggatcgg actcataatc
      601 ggtaaattgg ccaacaatca gaccggaaat tttttcaaaa attccaccta tgcgtaagtt
      661 ttgaatcatt ctgtcgactt ggtaaggctt ttctcctgta tcttcaacaa acaaaacggc
      721 atcctcaaag ggcaaatcga aagaagtacc gcgcaatccc attaaaaccg acaaatttcc
      781 acccagcagt ttggcggtaa tttttccctg acgattgagt ggatgaaaag gaagttgata
      841 aacaggcaat ctcccgaaca aaatttctct caaatgaaac actgcatcag aggttctggg
      901 caattcggca agatgtttgg ccattatacc gtgaatggaa gcaataccca tcctgtttac
      961 tgctgcatgc aagatggaaa tatcgctgaa tccgatcaac catttaggtg attttttaaa
     1021 ttgctcaaaa ttgattttgt cgataatttg caccagccca taaccaccgc gactcatgaa
     1081 aatggctttt acctccggat catcaagcgc ctgttgcaaa tcaaagattc tttcctcttt
     1141 tgttccggca aagcgaccat aggcagaacg tgcatatttc ccctcgagga ctttcaaatt
     1201 ccattcactt aaagttcttt ttgctccttc aacgtaggaa ggatggatag cgcttgccgg
     1261 agaaacaatt tgaaccatat ctcccgattt caaaaaagga ggcatcataa gcaataatga
     1321 gattttttat caataattga accatcacaa actaattaaa ccacaggaat ctgacgtatg
     1381 aataatcaat agttcaacca tttccaaagt tctttccatg aagttttttt accatacatt
     1441 aaaattcctg ttctgtagat tttccctgcc acccaagtgg tagcgatgaa ggaaagaatc
     1501 agaattgcca gcgaaagaac aatttcccac acggccacgc cataaggcaa acgaaccatc
     1561 ataactaccg gcgaagtaaa gggaatcagc gaacaccaga acgtcaagga ggaatc
//
LOCUS       DAOSCH010000206         1607 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_51465_length_1607_cov_7.200387, whole genome shotgun sequence.
ACCESSION   DAOSCH010000206 DAOSCH010000000
VERSION     DAOSCH010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51465_length_1607_cov_7.200387"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLZ54_09225"
     CDS             complement(<1..238)
                     /locus_tag="PLZ54_09225"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOJ67139.1"
                     /translation="MAFLYDIIVQEFGKRAISQVTVPNHITDNLKFEQRPYQVEAFQR
                     FILCYSENFEGKPKRPFHLLYNMATGSGKTLIMAG"
     gene            complement(238..1269)
                     /locus_tag="PLZ54_09230"
     CDS             complement(238..1269)
                     /locus_tag="PLZ54_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HOJ67140.1"
                     /translation="MLPEKKKSEFLLYQSDDGTIKINVRLENNTVWLTQADMVELFQS
                     SKSNISEHIKNVFDEGELNPDSTVRKFRTVRLEGNRKVEREIEHYNLDVIISVGYRVK
                     SFRGTQFRIWATERLREYLIKGFTMNDDLLKQGGGYFEELLERIRDIRSSEKIFYRKV
                     LEIYATSIDYNPNAEITQQFFQTVQNKLHWAAHGHTAAEIIYQRANAQLPFMGLTTFK
                     GKKPTKQEITVAKNYLTEEELQVLNRLVSAYLDIAEINAMQHKPMYMKDWIEILDGFI
                     KMSRQDVLTHAGKISAELAQQKALAEYETYKSKSVDELSEVEKQFIASIEQADKKLKA
                     LKPKNKKKE"
     gene            complement(1273..>1607)
                     /locus_tag="PLZ54_09235"
     CDS             complement(1273..>1607)
                     /locus_tag="PLZ54_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67141.1"
                     /translation="GGGSFVYLELKKYNQTFIEQIENAKDTDTLLQIWEQMKVKSFFK
                     YSVDLQEFQASIEEFKQFELNKQKEILCELLDKNQLYVNLSSLGDADFACTEEEKKVT
                     KDFYQMKK"
BASE COUNT          411 a          328 c          292 g          576 t
ORIGIN      
        1 aacctgccat tatcaaagtc tttccgcttc ctgttgccat attgtaaagc aggtggaacg
       61 gtcttttggg tttgccttca aaattttctg aatagcaaag tatgaaacgc tgaaaagcct
      121 caacctgata gggacgctgt tcaaatttta aattgtcggt tatatggtta gggacagtaa
      181 cttgggaaat agctctttta ccaaattcct ggactattat gtcgtataaa aaagccatta
      241 ctctttcttt ttattttttg gtttcaatgc ttttagtttt ttgtcagctt gttcgatact
      301 tgctataaac tgtttttcaa cttccgatag ttcatcaacg gatttgcttt tgtaggtttc
      361 gtattccgcc aatgcttttt gttgtgctaa ttctgccgat atttttcctg catgggttaa
      421 cacatcctga cggctcattt ttatgaatcc gtccagaatc tcaatccaat ctttcatata
      481 cataggcttg tgttgcatgg cgttgatttc agcaatatcc aaataggctg atactaaacg
      541 gttcaacact tgtaattctt cttcggtaag gtagttttta gccaccgtaa tttcttgttt
      601 ggttggtttt ttccctttaa aagtggtaag acccataaat ggcagttgag cattggctct
      661 ttggtaaata atttcggctg ccgtatgccc gtgtgccgcc cagtgcaatt tgttttgaac
      721 ggtttgaaaa aattgttggg taatttcggc attggggtta tagtctatgc ttgtggcata
      781 tatttctaac actttgcgat aaaaaatctt ttctgatgaa cgaatatccc ggatgcgttc
      841 cagcaattcc tcaaagtagc caccaccttg cttaagcaaa tcatcgttca tcgtaaagcc
      901 tttgatgaga tactcccgaa gacgttcggt agcccagata cgaaactgcg tgccacgaaa
      961 cgattttact ctgtatccta cggaaatgat aacatctaaa ttgtaatgct caatttcacg
     1021 ttctacctta cggttaccct ctaatcgaac tgttcggaat ttccgaacag ttgaatctgg
     1081 atttaattct ccctcatcaa atacattttt aatatgttcg ctgatattgg attttgatga
     1141 ctgaaaaagc tctaccatat cggcttgcgt aagccacacg gtgttgtttt ccaagcggac
     1201 gttaatttta atcgtcccgt catcggattg gtataataaa aactcgcttt ttttcttttc
     1261 aggtaacatc gcttactttt tcatttggta aaaatctttg gttactttct tttcttcttc
     1321 ggtgcaagca aagtcggcat cacccagcga agaaagattt acatacaatt ggtttttgtc
     1381 cagcagttcg cacaggattt ccttttgctt gttcagctca aactgcttaa actcttcaat
     1441 gcttgcctga aactcctgca aatccacact gtatttaaag aaacttttaa ctttcatttg
     1501 ttcccaaatc tgcaacagtg tatcggtatc ttttgcattc tcaatctgct caatgaaagt
     1561 ttgattgtat tttttgagtt cgagatagac gaatgagcca ccacctt
//
LOCUS       DAOSCH010000207         1600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_51822_length_1600_cov_2.891909, whole genome shotgun sequence.
ACCESSION   DAOSCH010000207 DAOSCH010000000
VERSION     DAOSCH010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51822_length_1600_cov_2.891909"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /gene="cas1b"
                     /locus_tag="PLZ54_09240"
     CDS             <1..906
                     /gene="cas1b"
                     /locus_tag="PLZ54_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005806445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1b"
                     /protein_id="HOJ67142.1"
                     /translation="PRYLPVENIAEFYVFGALNANSALYNFLGRNDIAVHFFDYYENY
                     TGSFMPRDGLLSGKMILAQTSHHHNKNKRLQLARKFVEGAAFNINKNLRYYNTRGKDL
                     DGVIETIEQYASQIPNTSSVDELMGLEGNIRQTYYSGFELIINDFSMGGRSRQPPQNE
                     VNALISFGNMMCYTQCLQAIHQTQLNPTISYLHSPGERRYSLSLDISEIFKPILVDRV
                     IFKLLNKRELQEKHFESKLNKCLLNPTGKKIFVQAFEERLNETIQHRSLKRKVSYRHL
                     IKLECYKLSKHLLGMEEYKPFKMYW"
     gene            908..1171
                     /gene="cas2"
                     /locus_tag="PLZ54_09245"
     CDS             908..1171
                     /gene="cas2"
                     /locus_tag="PLZ54_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOJ67143.1"
                     /translation="MYVILVYDVGEKRVGKMLKLCRRYLNWIQNSVFEGEISEVKLKE
                     LTILAKRMMNLEKDSLIIFSSRTEKFLDKQIIGKERSSINNFL"
BASE COUNT          553 a          287 c          283 g          477 t
ORIGIN      
        1 cctcgatacc ttccggttga aaatattgcc gaattttacg tcttcggtgc tctcaacgcc
       61 aacagtgctc tgtacaattt cttaggtcgg aacgatatag ccgtacattt tttcgattat
      121 tacgaaaact acaccggttc gtttatgcca cgcgatggtt tattgtcggg aaaaatgatt
      181 ttggcacaaa catcccacca ccacaataaa aataaacgat tacaactagc ccgtaaattt
      241 gtggaaggag cggcattcaa cataaataaa aatttgcgct actacaacac acgagggaaa
      301 gacttggatg gggtaatcga aaccatagaa caatatgctt cgcaaatacc caacacttca
      361 tcggttgacg aattaatggg actggaagga aacatccgcc aaacctatta tagcggtttt
      421 gaacttatta tcaatgattt cagcatggga ggcagaagta gacaaccacc tcaaaacgaa
      481 gtaaatgcgt taatttcgtt cggcaatatg atgtgttaca cccaatgtct gcaagccatt
      541 catcaaacac aattgaatcc tactatcagt tatttgcatt cacccggaga acgaaggtac
      601 tctttatcgt tggacatttc tgaaattttc aaacccatac tggtcgacag agtaattttt
      661 aagcttttga acaaaagaga acttcaagaa aagcatttcg aaagcaaact caataaatgt
      721 ttgcttaacc caacgggtaa aaaaatattc gtacaagcgt ttgaagaacg gctcaatgaa
      781 accattcaac atcgttccct taaacgaaaa gtaagttacc gacatcttat caaattggaa
      841 tgttacaaat taagcaaaca cttattggga atggaagaat acaaaccctt taaaatgtat
      901 tggtaaaatg tatgtgattt tggtatacga cgtaggcgaa aaacgagtag gtaaaatgct
      961 gaaattatgc agaagatatt taaactggat acaaaattcg gttttcgaag gagaaatttc
     1021 cgaagttaaa ttaaaagaat tgactatatt ggcaaaaaga atgatgaatc ttgaaaaaga
     1081 ttcactgatt attttttcga gccggacaga aaaatttctc gataaacaaa ttattggtaa
     1141 agagcggagt agtataaata atttcctttg actcgattgt cgaacgatgg ggttttttag
     1201 ttttgcgcga gcaaaaagag aaactgcgat cttttacatt ttgaaaatca atataaaaac
     1261 aagttgtcga taatcggcaa ttttcatatc tttgcctatc gacaacttaa tagtatcaga
     1321 aacgaaattc aattattctt tataggctga ttttcagcct aatttttcgc ctatttcaac
     1381 ggcttttaat cgtaccagaa ggaattgaaa ttatgctaac caatcttctg gcatttgcat
     1441 atattgcttt taatcgtacc agaaggaatt gaaattgttt ttcagatcat taatcttttt
     1501 cgatacatca ccttttaatc gtaccagaag gaattgaaat ttctatcctt attgcgcttc
     1561 ccgttcgagt tataatactt ttaatcgtac cagaaggaat
//
LOCUS       DAOSCH010000208         1565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_53510_length_1565_cov_5.215894, whole genome shotgun sequence.
ACCESSION   DAOSCH010000208 DAOSCH010000000
VERSION     DAOSCH010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53510_length_1565_cov_5.215894"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            128..331
                     /locus_tag="PLZ54_09250"
     CDS             128..331
                     /locus_tag="PLZ54_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOJ67144.1"
                     /translation="MALLYRKRWGIELLFKKMKQNFQLHFFYGENEMAIHTPNTVYID
                     SSTVIRCCKRKPAQKRHFALWQH"
     gene            691..>1565
                     /locus_tag="PLZ54_09255"
     CDS             691..>1565
                     /locus_tag="PLZ54_09255"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl glucosamine 2-epimerase"
                     /protein_id="HOJ67145.1"
                     /translation="MSVSTNQQINKSTIKLLKVLTVVGTRPEIIRLSRVMAKLDESEA
                     IEHVTVHTGQNYDYELNEIFYNDLEIRKPDYFLNAAGKNATETAGQILINIDPVLEKE
                     QPDAFLVLGDTNSCLCAIAAKKRHIPIFHMEAGNRCFDQRVPEESNRKIVDHIADMNL
                     TYSSIAREYLLREGLPADRIIKTGSPMYEVLHYYIPKIQQSTILEQLVLEKEKYFVIS
                     AHREENINSEKNFFRLVEILNLIADHYGFPVIVSTHPRTRKMIEEKQVRFHSLVQLMK
                     PMGFSDYNQLQMNAYA"
BASE COUNT          548 a          272 c          301 g          444 t
ORIGIN      
        1 tagtattttt gtttttccac aaacataaaa attatatggg taaaaatacg gaaataaatt
       61 tagtcggaca gctgattttt aaacaaatta tcgatttatt gggagaataa ttttgatata
      121 gaggacagtg gcacttctgt acaggaaacg ttggggaatt gaattgcttt tcaagaaaat
      181 gaagcaaaac tttcagttgc atttctttta tggggagaat gaaatggcaa tacacacacc
      241 aaatacggtg tacattgata gctcaactgt tatacggtgt tgcaaaagaa agccggcaca
      301 aaaaaggcat tttgcattgt ggcaacatta gtatggatac attgatcagc ttgttggatg
      361 tttataattg atcaggtgaa caaaaaaagc ttttgaaaag aatcaaaaag cttcaccttc
      421 cacaggtcag ctaaccctga acctgcaatt ttgagaggag gtctcttttg aaaatgaaaa
      481 ttaaaagtcc tattaatcgg ggttgcatgt ataaagatcg actaaatgaa agttttgtcg
      541 gataatagtg ataattttat attatacaca aacgattttt ttaaaactta aaaatgtagt
      601 tgaaatataa cgaaacaaac gtcaaacctg ttaacagatt attagtataa acagaacaaa
      661 attattcgat tatcaaatca actcatcaac atgtctgtat caacaaatca acaaatcaac
      721 aaatcaacca tcaaacttct gaaagtactt actgttgttg gtacacggcc ggaaatcatt
      781 cgtctttcgc gtgtcatggc caagctggac gaatcagaag ctatagaaca cgttaccgtg
      841 catacggggc aaaactacga ttacgaatta aacgaaattt tttacaacga tttggagatt
      901 cgcaagccag actatttcct gaatgcggca ggaaagaatg ccactgaaac agccggacag
      961 attctcatca acattgatcc ggtgctggaa aaagaacaac ctgatgcttt tctggtattg
     1021 ggcgatacca actcgtgcct gtgtgccatt gcagccaaaa aacggcatat tccaatattc
     1081 cacatggaag caggcaaccg atgtttcgat cagcgcgtgc cggaagagag caatcgcaaa
     1141 atcgtggatc acattgcaga catgaacctg acatacagtt ccattgctcg agaatattta
     1201 ttgcgtgaag gtttaccggc tgatcggatt attaaaaccg gcagtccaat gtatgaagtg
     1261 ttgcattatt atatacctaa aattcaacaa tccactattt tggaacaatt ggtattggaa
     1321 aaagagaaat attttgttat ttcagcccac agggaagaga atattaattc agaaaaaaat
     1381 ttcttccgtc tggtggagat actcaattta attgctgatc attatggttt tccggtaatt
     1441 gtatctactc atccacgaac ccgtaagatg attgaagaaa agcaagtacg ttttcattca
     1501 ctggtacaat taatgaaacc aatgggattt agcgattaca accaactgca aatgaatgcg
     1561 tatgc
//
LOCUS       DAOSCH010000209         1562 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_53668_length_1562_cov_3.796284, whole genome shotgun sequence.
ACCESSION   DAOSCH010000209 DAOSCH010000000
VERSION     DAOSCH010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53668_length_1562_cov_3.796284"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PLZ54_09260"
     CDS             complement(<1..277)
                     /locus_tag="PLZ54_09260"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HOJ67146.1"
                     /translation="MASNTFKFSGTGVAIVTPFRSDDSIDFTALRNLVDFQIKNKIDY
                     LVVLGTTGESVTLSADEKRAVVDTVLEAANGRVPIVVGIGGNNTQDII"
     gene            complement(280..831)
                     /locus_tag="PLZ54_09265"
     CDS             complement(280..831)
                     /locus_tag="PLZ54_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67147.1"
                     /translation="MLRKIRTIVGEKILRKKLRTQKRSRCIYDFHRAQLVGVLFKVNT
                     INEFQLIKDFLHYLRELDNKVIALCYIDSKKIPDYYLLVKGFNFFTRKSVNFFYIPRH
                     PMVNDFIEQPFDMLIDLTIDDIFTIKYIGILSKAKYKVGMRQKRMEKHYDLMIDIEKN
                     RSIANLIESIKHYIPIFASNEMK"
     gene            complement(900..1514)
                     /locus_tag="PLZ54_09270"
     CDS             complement(900..1514)
                     /locus_tag="PLZ54_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOJ67148.1"
                     /translation="MKIICLANNYRDHAKEINHPIPSVPVFFLKPDTALLRNNRPFFL
                     PDFSEEIHYEVELVVKISRLGKGIQEKYAHRYYSTIGVGIDFTARDIQRECVAKGLPW
                     EIAKAFDYSAAISPTFVSIEHFPDKQNIHFRLDINGQTVQSGCSGEMIFSFDTAIAHI
                     SKYMTLRTGDLLFMGTPPGVGRIKIGDRLQAYLENQLMLDFLIK"
BASE COUNT          488 a          312 c          256 g          506 t
ORIGIN      
        1 taataatatc ttgcgtattg ttgcctccaa tacctactac tattggtact cttccattgg
       61 ccgcttctaa aacggtatca acaacagccc gtttctcatc agccgatagg gttacagatt
      121 ctcccgttgt acccagcact accaaataat ctatcttgtt ctttatctga aaatcaacca
      181 aattacgtag tgctgtaaaa tcaatactgt catcagatct gaaaggagtc acaatggcta
      241 ctcctgtgcc cgaaaactta aaggtattac ttgccatttt tacttcattt cgttacttgc
      301 aaaaatagga atatagtgtt tgatgctttc aattaaattc gctatcgaac ggtttttttc
      361 aatatcaatc atcaaatcat aatgtttttc cattcgtttc tgcctcatgc caactttata
      421 tttcgccttc gatagtattc ctatgtattt tatggtaaaa atatcatcaa tggtcaaatc
      481 aatgagcata tcaaagggct gttcaataaa atcattgacc atcggatgac gggggatata
      541 aaaaaaattg acagatttcc gcgtaaaaaa gttaaatcct tttaccagca aataataatc
      601 aggaattttt ttcgaatcga tgtaacaaag tgctatcact ttattgtcaa gctctctcag
      661 ataatgcaaa aaatctttaa tgagttgaaa ctcattgata gtattgactt taaacaatac
      721 accaacgagc tgtgcacggt gaaaatcata gatgcacctg cttcgctttt gcgttctgag
      781 ttttttcctc agaatttttt cgcctacgat ggttctaatt tttcttagca agttctattt
      841 tttggcaaaa ataataaaat attattgtta ataggtagag gtcatcaatc attacgcagc
      901 tactttatca aaaaatcgag catgagttga ttttcaagat atgcttgcag acggtcacca
      961 atctttatcc ggcctacccc tggaggagtt cccataaaga gcaaatcgcc tgttcgtagc
     1021 gtcatatatt tcgaaatatg tgcaatggct gtatcaaacg aaaatatcat ttcgcccgag
     1081 caacccgatt gcaccgtttg cccgttaata tcgagacgaa aatgaatatt ttgtttatct
     1141 ggaaaatgtt ctatggaaac aaatgtaggg ctaatggcag ccgaatagtc aaaagctttt
     1201 gcaatttccc agggtaagcc ttttgcaaca cactcacgct gtatatctct tgctgtgaaa
     1261 tcaatgccta ccccaatggt tgaatagtac cggtgtgcat acttttcctg gattcctttt
     1321 cccaatcggg aaatcttcac taccagttcc acttcatagt gtatctcctc agaaaagtcg
     1381 ggcaagaaaa aaggacgatt attcctgagt aatgccgtat caggttttaa aaaaaatacc
     1441 ggtacactgg gaattggatg gtttatttcc tttgcatgat cccggtaatt gtttgctaaa
     1501 caaataattt tcatccgaaa ataatttatt aaatcaaaca ttatcagcat attaaaaaaa
     1561 ca
//
LOCUS       DAOSCH010000210         1561 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_53730_length_1561_cov_3.397742, whole genome shotgun sequence.
ACCESSION   DAOSCH010000210 DAOSCH010000000
VERSION     DAOSCH010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53730_length_1561_cov_3.397742"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1311)
                     /locus_tag="PLZ54_09275"
     CDS             complement(67..1311)
                     /locus_tag="PLZ54_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67149.1"
                     /translation="MKKITLSIVGLFLFAAAWAADFTPGNLVVLRVGDGSSTLSNAST
                     AIFLDEYTTDGTLVQSVDIPTSGENLLTNSGSATSEGQLTLSPDQSLLALAGYNVEPG
                     TTGVASTDAATVNRKIISVDYTGTVKNALLSATAYNGNNIRGAVTNGVDFWASGTATT
                     VAAGGVQYFGTGTPTQVSSTSTNIRVINIFNGQLYYSSSAKTGYGIFAVGEGMPIVSG
                     ITSTLIINMPQVSSTNASPYAFSFNATSTVAYIADDNSTSKGGGIQKWTFSDGNWSKA
                     YTLGATGFRGLTVDWSATNPKIFATGTDNNLYSVTDDGSGTSTPNIIASGATNTAIRG
                     VAFVPIRKTESHVVTPIIASMKILNNEISFDVLPQSDIQIYSITGSRIASYLPAKVIE
                     LNLTKGIYIIKVENQSAKIVLK"
BASE COUNT          493 a          275 c          293 g          500 t
ORIGIN      
        1 ttattacgaa atttgtctaa ccaaaaagtt gcatttgtga cttgaattca gcatttaaaa
       61 agttatttac ttcaaaacaa tttttgccga ttgattttca acttttataa tataaattcc
      121 ttttgttaga tttaattcta tgactttagc aggaagataa gatgcaattc ttgaacctgt
      181 tatggaataa atctgaatat ccgattgtgg aagcacatcg aaggaaattt cgttgtttaa
      241 aattttcatt gaggctataa ttggagtaac tacatggctt tcagtttttc tgatgggaac
      301 aaaagcaaca cctctgattg ccgtattagt agcgccggaa gctataatat taggagtaga
      361 agtccctgat ccatcatctg taacggaata aagattatta tcggtaccgg tagcaaaaat
      421 tttcggattt gtagcagacc agtccacagt aagtcctcta aatcctgtgg ctcctaacgt
      481 gtatgcttta gaccagtttc cgtctgaaaa tgtccatttc tgaataccac cacctttgct
      541 tgttgagttg tcatcagcaa tataagctac tgtagaggta gcgttaaatg aaaaagcata
      601 aggtgaagca ttggttgatg aaacttgtgg catattaatt atgagcgttg aggttatccc
      661 ggaaactatt ggcattcctt ccccaacagc aaatatcccg tatccggttt ttgctgaaga
      721 agaataatat aattgtccgt tgaagatatt gataaccctg atattggttg aagttgatga
      781 aacctgagtg ggtgttccgg ttccaaaata ctgaaccccg cctgctgcta cggttgttgc
      841 tgttccggaa gcccaaaaat cgactccatt tgtaactgca ccacgaatat tgtttccatt
      901 ataagccgtt gcagacaata atgcattttt aaccgttcct gtataatcaa cggaaataat
      961 ttttcggttg actgtagcag catcagttga cgcaacacct gtagttccgg gttcaacatt
     1021 gtaaccggcc aaagccaaga gagactgatc aggtgatagg gtaagttgac cttctgaagt
     1081 agctgaacct gaattggtca aaagattttc acctgaggtt gggatatcca cagattgaac
     1141 cagtgtaccg tcggttgtat attcatccaa aaagatagct gtagaagcat tggaaagagt
     1201 agagctgccg tcaccaacac gaagaactac caaattgcct ggagtaaagt ctgcagccca
     1261 tgctgcggca aaaagaaata atccgactat tgacaaagta atttttttca tgatcattta
     1321 tttcaagttt aaaccatatt gagtaaattt ctctacaaat atagtgattt aaatgttaat
     1381 gcagtatttt ttgtctgcaa ttttctattc gatggaaatt aattttctaa aagttaaatt
     1441 cagtcatgaa aacaaaaaca atttgtactc tgttaccaaa atgaaataat cgatttattg
     1501 ttttatccca aattaagctt tcaaaaatag aaaatttcat tttttcagat cgaattgctt
     1561 t
//
LOCUS       DAOSCH010000211         1543 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_54630_length_1543_cov_4.793011, whole genome shotgun sequence.
ACCESSION   DAOSCH010000211 DAOSCH010000000
VERSION     DAOSCH010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54630_length_1543_cov_4.793011"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..573)
                     /locus_tag="PLZ54_09280"
     CDS             complement(187..573)
                     /locus_tag="PLZ54_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67150.1"
                     /translation="MCQAAIAKPLLAAGLSHNTNKMEQEKNCAFQYAKMKDGIFRDTK
                     IVCDKSELTLDEAKKLWNEHFVDAAKWIKEGNTVEMVIWINMATPQSYGDTLQYISTD
                     AESDGVSIWETKKSYFKQYADAMVSA"
     gene            complement(639..1154)
                     /locus_tag="PLZ54_09285"
     CDS             complement(639..1154)
                     /locus_tag="PLZ54_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67151.1"
                     /translation="MAKILNISQILVYYDFPEIFLAKDIVETNYLCLLVDANETATTY
                     ISTAISQQRLSAFINGQIDLRDIYTNPEIEEWLIFNQVDQIIVAEKWNEPAIPEIYLP
                     EPGFVFQKQLQGLTPEEIEIELKKSDAMEKVSKQIIDVSRLVLDASNLKFKILERGYD
                     GKNFNLLDSPQ"
     gene            complement(1260..1382)
                     /locus_tag="PLZ54_09290"
     CDS             complement(1260..1382)
                     /locus_tag="PLZ54_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOJ67152.1"
                     /translation="MAELFEFELKKNNEPIETTNINSIRVYVSDEQKELMVSPL"
BASE COUNT          448 a          310 c          247 g          538 t
ORIGIN      
        1 ttagtgcttc gtatttagct tttcgtttaa ttaaccgcag ccagcttata acagcaccta
       61 tgcgccatta aaacgagcgc atagctgcat cacgattggc aaaataaaag ccgtcaagta
      121 tagtgcttaa cttcaacagt agcgcaaggc ttttacttcg ccaatcgccc accgttagcc
      181 gaaagcctaa gcagacacca tagcatcagc atactgcttg aaatatgact ttttagtttc
      241 ccaaattgac acaccatcac tttcggcatc agttgaaata tactgcaaag tatctccgta
      301 tgattgtggg gttgccatat taatccaaat aaccatttca acggtgttgc cttctttaat
      361 ccactttgca gcatcaacaa aatgttcgtt ccataacttc tttgcttcat caagtgttaa
      421 ttctgactta tcgcaaacaa ttttggtatc tctaaaaata ccgtctttca tttttgcgta
      481 ttgaaacgca caattttttt cttgttccat tttatttgtg ttgtgagaaa ggccagcggc
      541 taacagcggt tttgcaatag ccgcctgaca catctcggtt aataattagt ttcttctatg
      601 cggccatcgc aaagccgccc accgttacct gtaacatatc attgagggct gtccaataga
      661 ttgaagtttt ttccatcgta accacgttct aaaattttga attttagatt tgaagcatcc
      721 aaaacaagtc ttgaaacatc aataatttgt tttgatactt tttccattgc atctgatttt
      781 tttaactcga tttcaatttc ttcaggagtt aaaccttgta gctgtttttg aaaaacaaat
      841 cctggctctg gtaaataaat ctcagggatt gctggttcgt tccatttttc agcaactatt
      901 atttggtcaa cttgattgaa tattagccat tcttcaattt ctggatttgt gtaaatgtct
      961 cttaaatcaa tttgtccgtt tataaatgca cttagtcttt gttgcgatat tgcagttgaa
     1021 atatatgttg tagctgtctc atttgcgtca accaaaagac atagatagtt cgtctctaca
     1081 atgtcttttg caaggaatat ttcaggaaag tcatagtaga ctaaaatttg cgatatgttt
     1141 aaaattttag ccatctcaaa ttattggtgt cgaagctaca ttataattgg ttgttctcca
     1201 ccacgtgaag gaaaaacaaa aagaaaaagc caacgcactt ttaaaaacaa taaacatttc
     1261 tacaaggggg aaaccatcaa ttctttttgt tcgtcagaaa cataaacacg aatagaatta
     1321 atgtttgttg tttctattgg ttcattgttt tttttcagtt caaattcaaa taattctgcc
     1381 atcgcttttt tctttttgtt tttctgtttt aattttttag ttcaagctaa ataagtaatt
     1441 gctacccacg tcggccgcat ccaaaaccac ccgaatttcg ctgcccttga tataaaaagc
     1501 aacccctaaa cactcgctaa ttttagtaag gtcgtttgtc gag
//
LOCUS       DAOSCH010000212         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_56752_length_1502_cov_5.790601, whole genome shotgun sequence.
ACCESSION   DAOSCH010000212 DAOSCH010000000
VERSION     DAOSCH010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56752_length_1502_cov_5.790601"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(106..1398)
                     /locus_tag="PLZ54_09295"
     CDS             complement(106..1398)
                     /locus_tag="PLZ54_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOJ67153.1"
                     /translation="MNKVLIITYYWVPSGGAGVQRWVKFTKYLRKYGWEPVIYTPQNP
                     EYPALDHSLEKDVPSDVEVIKTPIWEPYNIYRNLMGKRNEPINAGFISENKKSGWKER
                     FSIWIRGNFFIPDPRRFWVKPSVKFLKKYISENQINAIVTSGPPHSMHLIGLGLKKFF
                     PTLPWLADFRDPWTNIDFYEDLHLTKIADRVHHRLEKKVVGNADAVVVVTKGMLEEFL
                     KLHPKRIEMIPNGFDEEDFRTVETELDKKFTISHIGTLNAARNPATFWKVLNELCVEN
                     EQFKSDLLIQLIGKVDYSVVEEINENNLKDNFVKIDYLPHNEAIAKQMSSQVLLLLIN
                     QTNNAKGIVTGKLYEYLAARRPILGIGPTNGEVSEILTSTQAGEMIDYDDAKKMKETI
                     VQYYEQYKNHSLTVPTDSVEKFSRKNLTHDLANLLDSL"
BASE COUNT          468 a          283 c          232 g          519 t
ORIGIN      
        1 ctgtatgaat gattttttct tctacaccaa ctgttggaaa ttggcgactc aacgttgccg
       61 ctttgataaa ttgcggacga gcaccaacaa ctgtaattat ttttctcata atgaatccaa
      121 taagtttgct aaatcgtgag ttaaattttt tcgggaaaat ttttcaactg aatcagttgg
      181 aactgttagt gaatgatttt tatattgctc ataatattga acaattgttt ccttcatttt
      241 tttagcatca tcataatcaa tcatttcccc cgcttgtgtg ctggtcaaaa tttcactaac
      301 ttctccattt gttggtccaa ttcccaaaat aggacgacga gcagctaaat attcgtataa
      361 tttgcccgta acaattcctt ttgcattatt tgtttgatta atcaataata aaagtacctg
      421 agatgacatt tgttttgcaa ttgcttcgtt atgaggcaaa taatcaatct ttacaaaatt
      481 atcttttaaa ttattttcat tgatttcttc tacaaccgaa taatccactt tgccaatcag
      541 ttgaatgagt aaatcagatt taaattgttc attttctaca caaagttcat tcaaaacttt
      601 ccagaatgtt gctggatttc tggcagcatt cagtgtacca atatgcgaaa tggtgaattt
      661 cttgtccaat tctgtttcga cagtacgaaa atcttcttca tcaaaaccat tgggaatcat
      721 ttcaattctt ttgggatgaa gttttaaaaa ttcttctaac attccttttg ttacaactac
      781 cacagcatca gcatttccta ccactttttt ttccaatcga tgatgaacgc ggtcggcaat
      841 ttttgttaaa tgcaaatctt cataaaaatc gatattggtc cacggatcgc gaaaatcagc
      901 cagccatggt aaagttggaa aaaacttttt caatcccaat ccaatcaaat gcatcgaatg
      961 aggcggacca ctcgtcacaa tagcatttat ttgattttca gaaatatatt ttttcaaaaa
     1021 tttcactgaa ggttttaccc agaaacggcg cggatcagga ataaaaaaat tgccacgaat
     1081 ccagatggaa aatctttctt tccagcctga ctttttattt tccgaaataa aaccggcatt
     1141 gattggctca tttcttttgc ccatcaagtt tcggtaaata ttgtacggtt cccaaatagg
     1201 cgttttaatc acttcaacat ctgaaggtac atctttttca agcgaatggt caagtgcggg
     1261 atattcagga ttttgaggtg tataaataac tggttcccag ccatattttc gcaaatattt
     1321 agtaaatttt acccagcgtt gaacgccagc tcctccactc ggcacccaat aataagtaat
     1381 aatcaaaact ttattcatgc taattctctt ttttgaggct ggagaatata ataacctgct
     1441 gttgtaaaaa aatttctaaa ataaggaata tcagcaaaaa cagagaaaag tttacgtggt
     1501 tt
//
LOCUS       DAOSCH010000213         1502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Paludibacteraceae bacterium isolate AMR_MDS_1862
            NODE_56753_length_1502_cov_5.318590, whole genome shotgun sequence.
ACCESSION   DAOSCH010000213 DAOSCH010000000
VERSION     DAOSCH010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863391
            Sequence Read Archive: SRR4236668
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Paludibacteraceae bacterium (wastewater metagenome)
  ORGANISM  Paludibacteraceae bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales;
            Paludibacteraceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/03/2023 22:44:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,860
            CDSs (total)                      :: 1,825
            Genes (coding)                    :: 1,800
            CDSs (with protein)               :: 1,800
            Genes (RNA)                       :: 35
            tRNAs                             :: 32
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 8 of 25
            Pseudo Genes (incomplete)         :: 16 of 25
            Pseudo Genes (internal stop)      :: 2 of 25
            Pseudo Genes (multiple problems)  :: 1 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Paludibacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56753_length_1502_cov_5.318590"
                     /isolate="AMR_MDS_1862"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2748031"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="36.29 N 139.38 E"
                     /collection_date="2015-05-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..1322)
                     /locus_tag="PLZ54_09300"
                     /pseudo
     CDS             complement(94..1322)
                     /locus_tag="PLZ54_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
BASE COUNT          443 a          276 c          300 g          483 t
ORIGIN      
        1 tctgaatatt atattcattc gtgtttgtgt atgtaataca tgtgggtttc atattctcta
       61 aaaatgatta ttgctattga aaattacaaa aaactatttc attaacagag aactgtctcc
      121 gtagctcaga aaacgaaaat cgtgagaaag ggcataatcg taaatttttc gccagttacc
      181 atcaacaaag gcagaaacca gcagtagcaa agtacttttt ggttggtgaa agttggtaat
      241 cattccgttg gtcagtcgga atgtgtaacc gggttgaata atcagttgcg tctgagcatg
      301 aagtacggag agatttcttt tctccatttc gttcaatatt aattgcaatg atgcttccgg
      361 agtcagagca cattcagtct ggtaaggcat ccattgcggg ataaaaaagt aaggatgctt
      421 ttcgtcggga aattgttgct tctcgataag taaaccaatg tggtacaaac tttccagcgt
      481 tcgtacggaa gtggttccca cggcaattat tttcccgata ttttttaaca gcttttctat
      541 tgtttttttt tgaatttcaa tgacttccgt gtgcatttgg tgatcggaaa tttgttttgt
      601 ttttaccggt tgaaaagttc cggcgccaac atgtaaagtc aattcggcag tctctatatt
      661 gcgggatttc aaactttcaa aaacttccgg ggtaaaatgc aatcctgccg taggagcagc
      721 caccgaacct ttaatttttg cataaaccgt ttgataggtt atcaaatcgc tttcttccgt
      781 aggcctgtgc aagtagggag gaataggcaa ttcgcctgcc ttttccagaa tttcggcaaa
      841 gtgaatattt tcattgttcc agctaaaacg aacaaaattt acattcccct ttctttcgag
      901 caattcggcg gagaactcaa attgcgttcc gtttatgtcg agttctttgg acaaagtgcc
      961 gtttttccat tttttttgat ttccgacaaa acatttccaa acacattctt tggtagccga
     1021 gagagataat gcgtaatcgg ccggctgagc tggctccagg caaaaaactt ctatcaacgc
     1081 gccagtttcc tgaaatgaat acgggcttgg ataacttttg tattgttgta aaccagcaat
     1141 gccttctcgg gcaaaaaaga agcaatattg ctaaaaacat tttcggtgat tgttccatct
     1201 ctgtaaatca gtaacttcga agcatcgcgc ttttttaacg gatacttggc aatgcgttct
     1261 tcgggtagga cgtaatcgaa atcatcaatg gaaaagtttg ttttttcttt taattcgttc
     1321 acgggagata caaaaatatt gttgtaattt tgtgcaaaga tacgaaaaat cgaaaaaatt
     1381 tccgatccgt tcgtatctcg ctgctacaaa agcagttcag aagataaaga tttaaaaata
     1441 tttttcgaaa aaccgaacaa catgataaaa gtaggagata aagtacgttt tctcaattct
     1501 ac
//
